LOCUS       DAIDFG010000001        39897 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_1_length_39897_cov_18.603710, whole genome shotgun sequence.
ACCESSION   DAIDFG010000001 DAIDFG010000000
VERSION     DAIDFG010000001.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 39897)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39897)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..39897
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1_length_39897_cov_18.603710"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         7348 a        13078 c        12531 g         6940 t
ORIGIN      
        1 gccggatcac cgggcgtcag ccgcaggatc aggaacacca cgacggccac caccagcagt
       61 accggaatgg tcgccagcag gcgttgcagc aggaatctca gcatgacaag cgttggagca
      121 ctctcttcac cagccggggc cgcggaaccg gcttagccgg gccgcaggcg cagcgccccc
      181 tcgggggggc agcgacgaca cgccagtgca gagcgtgggg gctctagttc tttttgatgt
      241 tccagtacac ctgcgcaccg cccggcacga gcccgctgat gtttttgcgc agcgccgccg
      301 gattgttgta ctgccccaga tgcacatgcg aggccgtctc gatggcacgc aattgcagct
      361 gctcggcgag ctttttcttc tcggcctcgg tgccggcctg ggcaaacttg accttcagct
      421 cttcaatgac cttgtcatcc tgccagccaa accagccctt gtcgccggtg gcgttaagca
      481 tggccatggt cagcgggttc tgaatgtcgc cggcggtcca ggccgtcatg aaggcattcc
      541 agccgccctt gtctggtgca tctttcttgg cccgacgggc cagcagcgtg gcccagtcgg
      601 actgctgcat gtccaccttg aagccggcgg cttcgagctg ctgcttggcc accagcggca
      661 acttggcaat cgaggccagg tcggtcggcc gcatcagcac gatgggctca cccttgtaac
      721 cagcctcggc cagcatctgc ttggccttgg cggggttggc catgccggtg tagatgccgg
      781 tgttgtcgct ggcatacggc gtgccgcagg ggaacatgct cttgcagaat ttgtacatac
      841 cgggcgcgcc cacctgtgtt ctcaggaaag cctcctggcc catcgccacc atggctgcct
      901 ggcggatctt cacgttgtcg aacggtgcat gcaggtggtt gaagcggaag atgtactgca
      961 ggccggccgg ctgggcgtcc acaatctgca catccttggc agccttcagc gaggcgtact
     1021 gttcgaaggc gggctgctca atgaggtcga cctcgccggc aatcagggca ttgaactgcg
     1081 tctgcggatc acggatgatg atccactcga cccgatcgag gtacaccacc ttgccgccgg
     1141 tggtgccgtc cggcgcttcg gcacggggct tgtacttgtc gttcttgacg tacaccagct
     1201 tctcacccgg cttgtactcg gccgtgacga acttgtaggg gccggagccg atgttttcct
     1261 tgatctgctc gctgccgggg gtggccgcga tgcgggcggg catgatgaag ggcacgttcg
     1321 acgagggttt gcccagcgcc tcgagcatca cgccgaaagg ctggttgaac ttcacgacaa
     1381 aggtcttggc attgggggtt tcgtagctcg ccaggctctt ggccatgaca ccgccgaaac
     1441 tgtcgcggct ggcccagcgc ttgagcgatg ccaccacgtc ctcactggtg acgggtttgc
     1501 cgtcgtggaa ctccaggccg tcacgcagcg tgaaggtcca gaccttgttg tccttggaag
     1561 cgctccaggt gtccaccatc tgcggcttga ccttgcctgc caggtcggtc ccgaacagag
     1621 tgtcataaat catgtacccg tgattgcggg tcacaaacgc ggtggtccag accgggtcca
     1681 gaatggtgat attcgaatgc ggcacgacct tgagcgtttt tgcctgggcc tgagccgcag
     1741 gcggcaacaa caggccggaa accagggcca ccgaagcggc cagggtgaaa agggagcgac
     1801 gtttcatgac aatttttcct tggcaacaag agttggggac agtcagacag caatcacaca
     1861 gctgctttgt aatgtaacca cacttctgat gcaatattca agccatatga aaaacaccac
     1921 accacccggc aaccccgtca ccgcctggac cgccaccgaa ctgtcgcgcg ccatccatac
     1981 acgccgggtt tcctgccgcg aagtgatgca ggcttacctg gagcgcatcg cccaggtcaa
     2041 cccgcgtcac aacgcactgg tcagcctgca ggagccgggc acgctgctgg cgcaggccga
     2101 cgaacgcgat gcccagctgg cgcccggcaa gtcgcgcacg ggaagcatgg gctggatgca
     2161 cggcatgccg caggccatca aggacatggc cgctgtgcag ggtctgccca ccacactggg
     2221 ttcgccgctg atgcgggatt ctgtcgcccg ggaggacggc ctcatggcgt cgcgcatgaa
     2281 ggccgcgggc tgcatcgtga ttggcaagag caacaccccg gagttcgggc tgggctccca
     2341 caccttcaat gaggtgttcg gcgtcacgcg caacgcctat gacccgtcaa aaagcgcagg
     2401 tggcagcagc ggcggtgcgg cggtggcgct ggccacacgc atgttgccgg tggccgacgg
     2461 cagcgacttc atgggcagcc tgcgcaaccc ggccgcctgg aacaacgtgt ttggcctgcg
     2521 gcccagccag ggccgtgtgc cgatgtggcc ggtgcaggat gtctacctga gccagttggg
     2581 gaccgaaggg cccatgggcc gaaccgtcga ggacgtcgcc aggctgctca gcatccaggc
     2641 cggctgggat gcgcgttcgc cactgtcgat tgcggaagat ggtgcccagt tcctgagctt
     2701 gcctgcactg gacaccaccc agctccggat tggctggctg ggcgacctcg gggcctacct
     2761 gccgatggaa gacggcatcc tgaccacctg cgagcaggcc ctgcggcgtt ttgagcagct
     2821 cggctgtgtg gtggaggcga cggcgctggg cacacccgcc gaccgggtct ggcaggcctg
     2881 gctggtgtgg cggcgtgccc tcgtcgcctc acgcattgcg ccgtttctgg ttcagggcag
     2941 caaccgcgaa aagatcaagc cggaagcgct gtgggaatac gaccaggccc agggcctgag
     3001 cgccagcggt ttttcagcgg caagtgcaca gcgcaccgcg ttttaccagc acatgctcac
     3061 gctgttcgag cggtatgact ttctggccct gccggcggcg caggtgtggc ccttcgatgc
     3121 gggcgagcgc tggcccaccc agatcaacgg tcacgccatg gacacttacc accgctggat
     3181 ggaagtggtg atttacgcca ccttcgccgg cctgccctgc atcagcgtgc ccgcgggctt
     3241 cggcccgcaa ggcttgccga tgggcctgca gctgatcggc agaccacgtg gcgaccgggc
     3301 cgtgctgcaa ctggcgcagg cttacgaagg tgcggcgcag gacatccttc agatcacgcc
     3361 gagccagcag gcgcgctgaa tcgccgacat cttgttgtcc accttgagtt tggtcatggc
     3421 gttggacagg tggtagttca ccgtacgttc ggcgcaatgc aggcgctcgg cggtctgacg
     3481 cgccgtcagg ccttcaaagg ccagcgccag gcattcgccc tcgcgcgggc tcagcgggct
     3541 gctggcctgg gccatcgtgc gcttgaacaa gggccagata ttgagggctg accagaccag
     3601 cgccgcagcc tcggcgcggt cgtgaaactg gcgtggactg aaaatgaaac actcgaaggc
     3661 ccgccccgat ggcaggggaa actcgatgcg aaccacactc tggaaaccgt aggccagcca
     3721 caagaggcgc cagcggctgg tgttgccggc ctctgccttg ccaatgtgct gccaggccac
     3781 gagggatgca tcagattcgc gccacggtgc gccgaaatcc tggctttctg ccagcgcgag
     3841 tgcagcgtcg ataaaacggg gcggatggac cgctgccacc agccggtcct gccgctggcc
     3901 aaacgggtcc gggctcagga ccaccaccgc ctcgacggaa aattcgcgca atgcagagat
     3961 ccagtcgctc aacatgctgt ccagcgcaat actgtcaaag ttgacagggg ggcgcatcgt
     4021 catttttgct ccggaactgt gacaatgtat tgtgatatgt ttgaaaattt acgcatgact
     4081 tccggagtac ccgccaggat caaacaagtg attcgcacgc tgtggagcag tcccggcgcg
     4141 tgggtgcgga tgtggtgtgc gcgaatcagg gctgaactgc tccaggagca gctggttctc
     4201 attttgcacc cgtcgcgctg gcgcctgcgc tggctcgggc tgttcacgct ggtgggacac
     4261 cccctgtttg ggtggatctg gggacagtgg ttgccgcagc cttacgaaag ccccgcgctt
     4321 cgcgctgtca tgggaacgct gggcttgctg ctgatctcgg agagcatcac caacgatcct
     4381 tccagcaaac tttcggcgcg gattttttcc gtcgttttct ggatccagct gccggttttt
     4441 ttcagctgga tgtatctttg caacaacggc aacacggtct ggctggccag cttttgcgcc
     4501 atggtgctga tttattacca tgtgaccgac tggcgtgcgg ccaccctggg gacgatcagc
     4561 ggcgccctgc tggcctggac gctgttcaag gcgttcggac cggccgttcc tgccatgagt
     4621 gagcagcagt tttccatcaa cgccgtcgtc atctcgttca gctgggccag cgccctgctg
     4681 ctgggactgt cgtcggcaaa cctgcggcga gagcacctgc agcacacgct gaccaccatg
     4741 ggcatcatgg cacacgagct gcgcacgccg ctggccacga tgtcgctgat aggcgacgct
     4801 gtccgcaatg aaagccggca ggtcaaggag cccggcggcg gccagcggct ggaccaactg
     4861 gtcgtcaggc tgcacaccct ggtgcgcaac atgaaccagc agatcgacac gcagattgcc
     4921 aacgcacgcc tgctgcgcct gccgacacac cgggaatccg tggctgccgc tgcactgctg
     4981 cgcaatgtgg tcaaggacta cccctatcgc aattcgcggg aacgcgaaag cgtggtgctg
     5041 gtgctacgca aggatttttc cttcgagtcg tcctatcggc tgttcgcgca ggtcatcaac
     5101 aacctgctca aaaacgcctt tcgcgcgctg gcggcaacca gttcggctgc caaacccggc
     5161 gacctgcgca tcgaagtcgg ggtgctcaat ggttacggac gcatcatcgt caccgaccag
     5221 ggcaccggca tcgcgccgga attgcaggca cgcatcttcg agccgttttt ctcgaccgat
     5281 cggggtaccg gtcatggcct ggggctggct ttttgccagc aggtcgtgga gggcgccggc
     5341 ggcaccatcc gggtgaagtc ggaacccggg cgcggcgcca tcttcatcat cgagcttccg
     5401 attctcaggt aacacaacca aacgcgcagc catgagtttt cccttgttcc atcgaccggg
     5461 cgccatcgtt tttcttgacg atgacccggc ttatctggag atgctggcga tggtgttgcc
     5521 gcgccagtgg cacgtcaagc tgtatctgcg gccgcagacg tgcatcaacc agctgcaaca
     5581 ggagccgccg ttgtgggaag ccgacgcatg gacgcagcag gagatgatcg agcgctggcg
     5641 tgctggcagt ccgctgattc cgcaagtcct gaactactgg gccagccata ccgaacgttt
     5701 cggactggcc agggtctgcg tgttcgacta cgccatgccc ggcatggacg gcctgcaggc
     5761 actgggcgag ttgatggact ggccgggcgg ccgggtgctg ctgaccggcc aggccgatga
     5821 acatgtagcg gtgagcgcct tcaaccgggg gctgatcgac cagttcattg ccaagcagac
     5881 cgtcgatatc tcccagcacc tgatcaccgt catccagcgc atgctggccc agccacaaat
     5941 gcgccattcg cagatctggc gcgccgcgct gacccagccc cagcatgccc tgctccaggt
     6001 gccttcggtg gctcgccagc tcgccagtct ggccagcagc cactgggtcg aacacgtggt
     6061 cattggccag cccttcggca tcctgggcat ggatgccgac gggcgcgcca gctggctgca
     6121 gctggagtct gccgcgggcc tggccgatct ggcggagctg gcagagtccc agggcctgaa
     6181 ggcggcggct gctgacgaca tccgggcggg ccgccacctg gtggacctcg agcttcagca
     6241 agccctcggg caacccgggc cggcgcggct gattccggcg ttttccattg gccatgaagg
     6301 caccgtgctg ggggccctgt ttccggtcga gctggccgcc agcccaccgg gattgaccgg
     6361 ctacaaacac tggctcaaga agcacggccg acgcaccgtg cgggactagg cttctggagc
     6421 ccgtcagcac aggccttaac tcgtttcagc cgtccgcgcc cgcctggcaa tcggccagtc
     6481 ccagggcttg accagtggcg cgatctcgca agcgaccgct tccacgtggc tgagctccgc
     6541 atcggtcagc cccgcgtgca gggtcaggcg caccatggcc cggttgcggc tggtcgccgg
     6601 ggcacagaac acagcaccaa aaacaccacg ttcctccagg tggtcgcgca gcaccatggt
     6661 gtcaggctcg gtgccggcct ccagggaaat gatctgctcc gtcccctggt ggatcgggta
     6721 acccatgtca gacagactgc cgcgcagccg caacgtgtgg gcccgcagac gctggcgttg
     6781 ttcatcgcgt ttgccgatca cggcgagtgt ggcgccgagg ccggcgatct cgtgcggcag
     6841 caggcaggag ctgaagatat tggggtagct ggaactgagg atgtagtaac gcaactcggc
     6901 gggcgccgtg aaaaaaccac cgcgcccggc aaaggccttg gccaggctgg ccgtgatgaa
     6961 atgcacccgg tccgtcagac cgagttcggc gcacaggccg gcgcctccgg gtccatgggt
     7021 gccgagcgaa tgcgactcgt ccaccaggat catgcagccg tggcgctcgg ccacttccac
     7081 catctcgcgc aggggacaca aggcgccggt ggtgctgtac accgagtcca ccaccaccac
     7141 acccggacca tcccgcgaca acacccggtc caggtggtcc gggtcgttgt ggcgaaacgg
     7201 atgtgccggt gcgcgcgccg catgcgcacc ttcccacagc gatgtgtgcg ccagggtatc
     7261 gaggtacacc ggggtttgcg ggtcggcaat cgcctgcagc aaacccacat tggccgcata
     7321 accggactgg cagacaaacc cgtcttcctt tccgacccac tgggccaggt ttttttccag
     7381 tgtccgggcg ggatgttcgc catgcacaaa cacgccggac tggatcacga actcctggtc
     7441 gttgcgcagg gccgccacct gggcgctgac gatgtcaggg tggccggtca cactgaggta
     7501 atcgttgccg tcgagccgaa cggcatccgg accggccgtt ctgccgtgaa ggacaaactt
     7561 gccaccccat tgctgctgcc agcggggcgt gaattcgcgc gcaatgcgct gctgcagatg
     7621 cggggagagg cccgggttgg taacagtttt tgttacgagt tgtgcagggg atttttcgag
     7681 aacttgagcg tgcatggtgg ttaccttgtg aaaaagtacc accattgaag atgaatttaa
     7741 caaattcagg gttgcccccc ctgtcaacat tgacaggggt cggtgcccga aagtcggatt
     7801 tcggtgtagc gattgcccta aatcactacc ttggtattac tctggtgttg cttggcgacc
     7861 actggtcagg cttaaagctg ccaatgccat gatttttgcg ctgtccacca gctcctgaat
     7921 gcccacatat tcgtcaggct ggtgcgccag atcgagaatc cctggaccgt aagcaataca
     7981 agcgtcgagc ttgccggtgc gcacgatgtg tttctggtcg taagtgccag gactggagat
     8041 gtagctggct tcgcggccca gcacccgggc aatggcacgc gcggtggcca gcaccaccgg
     8101 cgcgtccttg ggcgtggccg tcggcacaaa actcatgatg tcccggatac cgtagtcaaa
     8161 gccggggcgg ctgcgcttga gctcgtcgag catggccaca atctcggcct tcacggcctc
     8221 caggttttct tcagcgatga agcggcggtc cagcacggcg cggcatgaat gcgccaccac
     8281 cggtgccggc aggtcgcccg tggggcggcc gcgcgcgtcc agggaaaaca gctgctcgac
     8341 ctgtcctccg tgcaggctgt tgatgttgag cgtgctgacc ctggcgccgg gcggctccac
     8401 cggttcggcc gtatggcgcc cggacagccg cggctgcagc tgcgtctcca gcagatggat
     8461 gaaggcgctc atgtggttga tggcactgtc gcccagaaaa ggcatggagc catgtgcaat
     8521 ccgcccctgg gtctcgacct cgccccacca gacgccgcgg tgacccaggc agatgcggtc
     8581 cacgcccagt ggctcgggaa tgatcacatg gtgcacgcgc ggtttcgtga aataaccgcg
     8641 ctcggccaga taacccacac cggcaaagcc gcccgactcc tcatcgaccg tgccggaaat
     8701 ttcgagcgcg ccggcaaacg gcaggccttc ttcgagaatc gcctcgcagg caatcacgct
     8761 ggaagccagt ccgcctttca tgtcgcaggt gccgcggccg tagatcttgc cgtccctgac
     8821 ctcgccgccg aagggatcgg tggtccagcc ggaaccggct tccaccacgt cgatgtggct
     8881 gttgaagtgc acacactcac cccccggtgc gccttcgtag cgggccacca cattgatgcg
     8941 tggatgggag ttgcgatcgc ccggtgcacc gtcggcccgg atgtattcga cggcaaagcc
     9001 ccgttttttc agccgctccc caagcaggcg ggcgcaggct tcgtaagcgt cccccggcgg
     9061 gttgatggtg ggaatgcgaa ccaggtcctg ggtgagcgca atcacctcgt cgcgtttcga
     9121 ttcaatgcgt ttgagcaggc tgtcattgtt catgcagcca gtttatgtga accaagcccc
     9181 actgtctcgc ggcggttcca tacacggctc ggggaccgca ttttccaacc ggggtcgcgg
     9241 gtccggctac gccggcccgc aggcgcagcg ccccctcggg gggcagagag ctacacgaag
     9301 tgagcgaacg tgggggctct agttctgccg ccacggcagg ccgcggtagc gccaaccgcc
     9361 gcgtgcacca cgatgggcct gcgcatcggg gtcaccttca aaaccctcca ggatgttgta
     9421 ggcctccagc cccagttcgg tggcacgtct ggcggcggcg atcgagcgta caccgctacg
     9481 gcaaagcagc acggccttct ggccggccgg cacggcggtc tgcaggccct cgtcgaactg
     9541 cgggttcatg gccatgcccg gccactgttt ccaggccagc ggcaccgcac caggcacaaa
     9601 acccacccat tcacgctccg catcggtgcg tacatcaatc agcaccgcct ggccggcctg
     9661 ccaccactgg caggccagct cgggcgacac attgccggca tatccatcgg ccggcaggac
     9721 atggaacacg tcggccccac cggcatcatg gcggccgccc cccatgccgg aactctggtt
     9781 ggcaggaagc gcttcgtcca tgcgcgcggg tcggggcagc tggagctgcg ccatggtcgc
     9841 cacaaacccg gccaggcttt tgccggcgat gcgggcattg ccggccttct cggccgcaat
     9901 cgtcgaatgg gtccggccct ggtagtcgtg tcccggccag accaccgtgt cgtccggcag
     9961 tgtgaacagc cgcccggtga tgctttggta gagggcttcg gcgctaccgg actggaaatc
    10021 ggtgcggcca cagccattga tcagcagcgt atcgccggtg aagaggtggc cgcgccagag
    10081 gtaactcacg ctgccggccg tgtgccccgg ggtgtgcagg acgcggacct gttcaccacc
    10141 gacctgaagc acatcaccgt cgccgagctg tcgggcggcc gttgcaatgc cgcagccttg
    10201 cggcgccgcc gtgcgggcgc cggtgtgttc cgccagttgc ccggcgctgg tgatgtggtc
    10261 agcatgggca tgcgtctcca ccgtccacaa cagcttgagt ttgtgctccc gcagcaccgc
    10321 gaggtcgcgc tcgacctgct ggtccaccgg atcgatgatc agggcctcac gcgtggacgg
    10381 atcaaacagc agataggtat aggtgctgga ggcagggtca aaaagctgaa tgggtgtcat
    10441 gacaggtttc cagggcaggg gcaggcaaca ggccgaccgg catacacggc aagtttaaaa
    10501 cctcgcgcct ccctgctgca aagtttggcc cgactccagg ctgctccttt ttcagtagct
    10561 gcttgcgccc gtcggataat ggctacagcc aaaaacagct cataaaattt ccacgccccc
    10621 gatcgccggg ccaagcacca cgcaccagcc ccgaagcttg acggaaagct gacagggcac
    10681 gctttaacaa agccctgata aacagtttca ccccaattag gcgcaatctg cgccgggccg
    10741 ttggtaaaaa ccctcttacg cgtaaagcgt cgatgacaaa agatagtgcg ctaacgttct
    10801 ttcacaacag gagacaagcc atgaccgata tcaagacccc tcgccgccgc agcctgctca
    10861 agggtgcagc cgtcgccgct ggcgccggag ccatgtccgc accgatgctg gcaaccgcgc
    10921 aaaccgccac cacgctgcgc ttccagagca cctggccgtc gaaagacatc ttccacgagt
    10981 acgcctccga cttcgccaaa aaagtcaatg acatggcgag cggcaagctc aagatcgaag
    11041 tgttgcccgc gggcgctgtc gttccaccct tccagctgct ggaagccgtc aacaagggca
    11101 cgctggacgg cggtcacggc gtggttgctt accactacgg caagaattcg gcgctcgcgc
    11161 tgtggggttc cggcccgtcc ttcggcatgg accccaacat gctgctggcc tggcacaact
    11221 acggcggcgg caaggccctg ctggaagaga tctacaagtc catcaacatg gacgtggtgt
    11281 cctacctcta tggccccatg ccgacccagc ccttcggctg gttcaagaag cccatctcca
    11341 aggtcgctga catccagggc acgaagttcc gtaccgtggg gctggccgtc gacatgtaca
    11401 ccgacatggg cgcggcggtg aatccgctgc ccggcggcga gatcgtgccg gcgctggatc
    11461 gcggcctgat tgatggcgcc gagttcaaca acgcctcctc ggacaagctg ctgggcttcc
    11521 ccgacgtggt caagaactgc atgctgcaga gcttccacca gtccggcgag cagttcgaaa
    11581 tcctgttcaa caaaggcaag ctcaacgccc tgcctgccga actcaaggcc atcatcgact
    11641 acgccgttca ggctgccagc gccgacatga gctggaaggc catcgagcgc aactccaagg
    11701 actacattga cctcaagaag gccggtgtca agttctacaa gacgcccgac gccatcctgc
    11761 gcgcccagct tgcttcctgg gacaagatca tggtcaagaa ggggggcgag aacgcgctgt
    11821 tcaagaaggt gctcgactcg cagaaagcct ttgcacagcg cgcaggccag tggcagaacg
    11881 actacaccgt ggacttcaag atggcctata accactactt cggccgcggc aaaaaagcct
    11941 gagcggggcc acagcctctg aggggcacct gagggtgccc tttttgtttt ttctgggacc
    12001 gcctgggcgg cgcaaggagt tggaatgcaa tcgtttttac tggcggtgga ccgcttttcc
    12061 acctggatcg gcaaggcctg cgcctggagc gtggtcctgc tgaccgtgct gatcagctgg
    12121 gaggtgttca gccgttatgt gctcaaccgg ccccacgcct gggtgctcga cgcccagatc
    12181 atgctttacg gcctgctgtt catgatggcg ggcgcctaca ccctgagtaa aaacggccat
    12241 gtgcgcggcg acgtgcttta cggctttctc aggccgcgca cgcaggcgat cgtcgacctg
    12301 acgctgtaca tcatcttctt cctgccgggc gtcgtcgcgc tgacctgggc cggctggacc
    12361 tacgccaatg agtcgctggc gatccgcgag cagaccttca acgccgaccc gcttccggtc
    12421 tatccgttca agttcgtgat cccggtgtcg ggttttgtgc tgctgctgca gggcatcgtg
    12481 gaaatcatcc gctgcatcca gtgcatacag accggcgcct ggccggtgcg cgaaggcgac
    12541 gtggaggaag tggacgtgga aaaactcaag gagatggtgc acgtgaaaga cgaagacatg
    12601 gccgcgctcg agcgcgatct ggccctcaag gagaatcaca aatgaagatc cgcaaggaac
    12661 tctggttcgg cctgagcttc atggcgctgg tcatcatcgg cgccgccacg ctgcttctga
    12721 gcgtcgaaaa aatcaccaac gggcacctgg ggctgctgat gctgtccctg gtggtgaccg
    12781 ccatcatgct gggttttccg acggccttca cgctgatggg catgggcatg attttcacct
    12841 ggctggccta caaccgggac acaacgaaga cgctggacct gatggtgcag gcggccttca
    12901 agaccatggc cagcgacgtg ctgatcgcga taccgctgtt cgtcttcatg ggttacctgg
    12961 tcgaacgggc caacctgatt gaaaaacttt tcaagagcct gcatctggcc atggcccgga
    13021 ttccgggctc gctggccgtg gccacccttg tcacttgcgc gatttttgcc accgccaccg
    13081 gcatcgtggg tgccgtggtc acgctgatgg gcctgttggc attgccggcc atgctgcgtg
    13141 ccggttacag cgttcagctg gcggccggcg ccatcacagc gggcggttgc ctgggcatcc
    13201 tgattccgcc ttccgtgctg ctgatcgtgt acggcgccac cgcgggcgtc tccgtggttc
    13261 agctttacgc cggtgcgttt ttcccgggcc tgatgctgac cggcctgtac atcctgtacg
    13321 tgatcctgat cgccaagctc aagccgcagt gggcccctcc gctgtccgcc gccgatcgcg
    13381 tggtcacgct gccgcccctg acgcaacgcc tgaccagcag cacggccacc cacgcactga
    13441 caaccttgtt cagcgcgctc aagggaaaac gcaacaccga cgttccgctc ggccatatcc
    13501 tgcggcaact gacgattgtt gtgctgccgg gcctgctttt tctgctggtg ctggtgacca
    13561 gttaccgggc ggtcaccacc atcgaagccg aagcggtgta cgacatccag cagataggcg
    13621 cgtccagctc gtcagccgcc ccagacgccg atgccggcgg cttgcaggaa cctccccagg
    13681 aagacggcct gaaagagccg gttgccgagg gcggcttgca ggaccccccg tcaggcgatg
    13741 tcaaggagcc gccaggcgca gaagctcccg ccgccgcgcc ggcggccgcg agcgtagccg
    13801 aacctcccgc cgcagccaca gtcagcgccg acaaggcacc tgctggcgca accacacgcg
    13861 atgcgcccac ctggtggtgg gtctgctttg ccatctttgg cgcgctggtt gccctcttct
    13921 accttttcct gagttttgcg cggctggaaa tcttcaagat gctcctgacc tcgtttttcc
    13981 cgctgatgat tctcatcatc tcggtgctcg ggtccatcgt gatggggctg gccaccccga
    14041 ccgaagcggc cgcgatgggt gccttgggcg gttttctgct ggcagtggcc tacaagcggc
    14101 tcacgctgtc ggtgctgcag gagtcggtgt tcctgacggc caagacctcg gccatggtgt
    14161 gctggctgtt tgtcggctcg gccatcttct cggcggcttt tgccctgctg ggcggccagg
    14221 agctggtgga gacctgggtg ctcagcatga acctgagcaa gacgcaattc ctcgtgatga
    14281 gccaggcgat cattttcatt ctcggctggc cgctggagtg gaccgagatc atcgtgatct
    14341 tcatgcccat cttcatcccg ctgctggaca acttcggcgt cgatccgctg ttcttcggcc
    14401 tgctggtcgc cctgaacctg cagacggctt tcctgagccc gccggtggcc atggccgcgt
    14461 tctacctcaa gggcgtagca ccgaaacatg tgaccctgaa ccagatcttc gccggcatgt
    14521 tgcccttcat gggcatccag atcctggcca tcgtcctgct gtaccagttc cctgccatcg
    14581 gcctgtggtt gccgcagttg ctgtacaaat aagcgggcgc cgcccacctg tcgggaaaca
    14641 aaggggcgcg caggcgcccc tttgttttgg aaaaacggta agattgacgt ttacgtaaac
    14701 gtcaatcatc cacacggaga cagccatgtc caacggcaac agcaagcgcg cggccctgct
    14761 ggccccctgg caaccgccca ccaccgggcg gggccggccg tttgggctgt ccggactcaa
    14821 ggcctcggcc aagcccctgt cgagcgggga caaggacgca gacaaggccg acgtggaagc
    14881 cctggcgagc gagctggacg gcctgcagaa cctgttttac gccgacaagc gctacaagct
    14941 gctggtcctg ctgcaaggca ccgacacgtc cggcaaggac ggcacgattc gcgccgtgtt
    15001 cagccggacc agcccgctgg gcgtgcacac cgtaggctgg aaggcgccga gcgaggaaga
    15061 gcgggcgcac gactacctgt ggcgcatcca tcgccagatg cccggggcag gcgaaaccat
    15121 gattttcaac cgcagccact acgaagacgt gctggtcccg gtggtcaaag gctggatcac
    15181 gcccgagcag accgcgcagc gttttgccca catcaacgag ttcgaacgcc tgctggccga
    15241 aaccggcacg gtgatcctca agttcatgct gcacatcagc ttcgaggaac agggccagcg
    15301 cctgcaggaa cgggtggacg acccgaccaa gcgctggaag ttcagcatgg gcgacctgga
    15361 ggtacgcaaa cagtgggccg actaccagaa ggcctacgag gcgttgctgg gtgcaaccag
    15421 caccccttgg gcaccctgga ccgtggtgcc cgccgactcc aagacccacc gcaacctgat
    15481 ggtggccacg ctggtgcgcg acaagctgaa gtcgctggac ctgtgttacc cacctgacga
    15541 tccggcactg gccggattga agattgaata atttttccca tatccaagcc acgagacaac
    15601 tcctccagag agcaccgcca tgaccgacct gtccgccgaa taccaggccc ttgccagcta
    15661 ccgccccacg cacaaggtgc gttttgtcac cgccgcctcg ttgtttgacg gccacgacgc
    15721 cgccatcaac atcatgcggc gcatcctgca gggcatgggt gccgaggtga tccacctggg
    15781 ccacaaccgc agcgtggacg aagtcgtcac ggcggcgctg caggaagacg cgcagggcat
    15841 tgccatcagt tcctaccagg gcggccacgt cgagtacttc aagtacatgg tcgacctgct
    15901 caagagccgc ggcggcgagc acatccaggt gttcggcggc ggcggcggcg tgattgtgcc
    15961 gccggagatc cgcgagctcc atgcctatgg cgtctcgcgc atctacagtc ccgaagacgg
    16021 ccagaaaatg ggcctggccg gcatgattgg cgagatggtc atgcgctgcg acaaggacct
    16081 gaccggcttt gcccccgcgg acctgaaggc catcgaaggc catggcgaaa tggcctggcg
    16141 cgccttggcc cagctcatca ccgcgcttga aaacgacaag gcaaatcccg cgttggtcga
    16201 gacgctgcgt gcgcaagctg ctatcaaaaa gataccggtg ctgggcatca ccggcaccgg
    16261 cggcgccggc aagtcctcgc tgaccgacga actgatacgc cgcctgcggc tggaccagaa
    16321 cgaccagttg cgtgtagccg tcatcagcat cgacccctca cggcgcaaga gcggcggcgc
    16381 cctgctcggc gaccgcatcc gcatgaacgc catcagcccc tggcagcagg gtccgcgcgt
    16441 tttcatgcgc agcctggcga cgcgcgagtt cggcagcgag atcagcaagg cgctgcccga
    16501 agtgattgcc gcctgcaagg tggccggttt tgacctgatc gtggtcgaaa cctcgggcat
    16561 cggccagggt gatgccgcca tcgtgccgct ggtggacgtg cccatgtatg tgatgacgcc
    16621 cgagttcggc gccgccagtc agctcgaaaa aatcgacatg ctggacttcg ccgagtttgt
    16681 tgccatcaac aagttcgacc gcaagggctc gctcgacgcc ctgcgcgacg tggccaagca
    16741 ggtgcagcgc aacaaggaag cctggggcac cgcgccggac aaaatgccgg tgttcggcac
    16801 catggccgca cgtttcaatg acgacggcgt caccgcgctg taccaggccc tcaagccgcg
    16861 cctggccgga ctcgggctgc agttgtccga aggcgccctg cccgccgtcg atgtgcgcca
    16921 cagcaccaac cagacgccgg tggtgcccgc cgcccgcacg cgttacctgg ctgaaattgc
    16981 cgacacggtg cgcagctaca aaaaacgcgg cagggagcag gcgacgctgg cgcgcgaaat
    17041 ccagcaactg cgtgccgccg ccgacatgct gaccgtcggc aagcccgaca aggccagggc
    17101 cgcggaagcc gccatcgacc tggcggtgca gcgcgagcag gtccaggacc cggccgcacg
    17161 caagctgttg atccagtggc ccgcgatgca gcaggcttac gccggcgaag agtatgtggt
    17221 gaaaatccgc gacagggaaa tccgcaccgc gctgaccagc aagtcgctct cgggcacgac
    17281 catccgcaag gtggcactgc cgcagtacga agaccacggc gaaatcctca agtggctgat
    17341 gctggacaac gtgccgggca gctaccccta caccgccggc acctttgcct tcaagcgcga
    17401 gggcgaagac ccgacacgca tgtttgccgg tgaaggtgat gcgttccgca ccaacacgcg
    17461 tttcaagctg ctgagccagg gcatggccgc caagcgcctg agcacggcct tcgactcggt
    17521 cacgttgtac ggcaacgacc ccgacctgcg gcccgacatt tacggcaagg tcggcaacag
    17581 cggcgtgtcg attgccacgc tcgacgacat gaaggtgctt tacgacggct ttgacctgtg
    17641 cagccccagc acctccgtca gcatgaccat caacggaccg gcgcccacga ttctggcgat
    17701 gttcatgaac accgccatcg accagaatct cgaaaaattc agggccgaca atggccggga
    17761 accgacggac accgaaaccg aaaaaatccg cgaatgggtg caggaaaacg tgcgtggcac
    17821 ggtgcaggcc gacatcctga aagaagacca gggccagaac acctgcatct tctcgaccga
    17881 gttcagcctc aaggtgatgg gcgacatcgc cgagtatttt gtgcacaacc gcgtgcgcaa
    17941 tttctacagt gtctccatca gtggttacca catcgccgag gccggcgcca accccatcag
    18001 ccagctggcc ttcaccctgt ccaacggttt cacctttgtc gaagcctacc tcgcgcgcgg
    18061 catgcacatc gacgacttcg cgccgaatct gagctttttc ttcagcaacg gcatggaccc
    18121 ggagtacacg gtgatgggcc gcgtggcccg ccgcatctgg gccgtggcga tgaaggaaaa
    18181 atacggcgcc aacgagcgca gccagaagct caagtaccac atccagacct cgggccgcag
    18241 cctgcacgcg caggagatcc agttcaacga catacgcacc accttgcagg cgctgattgc
    18301 gatttacgac aactgcaact ccctgcacac caatgcgttt gacgaagcca tcaccacgcc
    18361 caccgaagag tcagtgcgcc gcgcgatggc gatccagctc atcatcaacc gtgagtgggg
    18421 cctggcgaaa aacgaaaacc cgtcacaggg cgccttcatc atcgaggaac tcaccgagct
    18481 gctggaagaa gcggtgctca gcgagttcga gcgcatcgcg gagcgcggcg gcgtgctggg
    18541 cgccatggaa accggctacc agcgcggccg catccaggac gagtccatgc attacgagat
    18601 gctcaaacac accggtgagc tgcccatcat cggcgtcaac accttccgca acccgcatgg
    18661 cgacgccgtg caggacaagc tggagctggc gcgctccacc gacgaggaaa agcagagcca
    18721 gctggcacgc ctgcaagact tccaccggcg ccatgccaag gaagccccgg ccatgctcaa
    18781 gcgcctgcag caggcggtga ttgccaatga caacgtgttc gaggtgctga tggacgcagt
    18841 acgcgtctgc tcactcggcc agatcaccca cgcgcttttt gaggtcggtg ggcagtaccg
    18901 gcggaatatg taggcttcgg cctcacggcc atccgcctgc gcccgatcgg acgcgttcac
    18961 tatgaattca ggagctgctg gcgcccgtcc accaagggtt ggaggccagt ttggcttcaa
    19021 aaccagaccc ggaccgtccg gcaatggcgg gggtggaatg tatgaccgtc atgtcgccat
    19081 ggacgcatga ggcaaaacag gagacagaac ccggccctga cgcgatgtca gtggcccggg
    19141 ggtgggcagc ttgcgctcaa gccgcccagg cgggcccagg gcttagccgc ctttgtgtgg
    19201 gcgcttgccg gggttctttt tgctgcgggt ccaggtgccg cgctccaggc tgatcttttg
    19261 accacggccg gccgtgcggg tcatgccagg ggcgggaacg ggcttgggtg cgcagatgtc
    19321 ggcttcgacg cggatttttt tggacatgga agactccgga ttaaaaaatt ggcagaaccc
    19381 ggcattttaa gctaccgcgc cccagtcggg gcaatgcagg gccagccggc cgtccggacg
    19441 cgatttacat ggcccccttc agcggcaggt tcagcagcag attctgcggt ttgagcttga
    19501 ccgactggcc ggcattcatc atcaggccca ggtagaccgg cttggcggcc acatcggcgc
    19561 gcatctcctg cggatccgca aaatccagcc ggaacaggct gaccaggcgc tgcaaacgtc
    19621 cggcctccac ctccctgcct tcgtacaaat cgttgagcag cgccgtggcc tccacgtcca
    19681 gccccacatg ccagcgccag gcgtcatctt caatcttttg caggggcttg atgctgacaa
    19741 caacgcccag gaaatgggcg acccatttct ccagcacgcg ggccagcgcc gtcaggcccg
    19801 agcgggcgcg cgtcatggtg aacaccaggc cgtggctcag gtcgttgctg atttcatgcg
    19861 tcaggtcgag caggaagtgg tgacggtcac tggcgggcca gtaatccggc gcgttggcat
    19921 ccgtcagcac ctgcatgtcg atggcccgca agggtgcctt ggcttctgcc aggaagcgcc
    19981 cgaagtcgcc cagcccgccg gtttcgttga gcatgtccag cacatcccgg tcgccggcca
    20041 gcacctggcc gtcctgcgtg gtgatgcgct gggcgcgaaa cagcatttcg gcggcacgca
    20101 cctcgtaggc atcggtggcc tcatccagca cctggcgcag gatggcctgg gccagcaggt
    20161 cgatgaacag cggcgggatg ctgatgctgc cgctgcggaa caggcccagg taatacgcct
    20221 ccagcgtgcc ggccgccagc agcccatcac gaaagcgcaa aaacaggccg taactctcgc
    20281 gggcgtcggc gtcctgcagg gcctgcagtt cggcctcggt caccggccgc gacggcgcgg
    20341 ccagcaaggc cgcatgcagc gtttgttcag ccgcgcagga ctcgggcacc agggccagtt
    20401 cgggtcggtt caggaacagg cgcaggtaat cgtcggtggg tgccagccag ccgcgtgagg
    20461 tgatctggag ctgatggaag ccgctggaag gccagaaatt ttgcatgggg gcaatcgtcg
    20521 cacattgcgc cgtcaggtca ttcgctatga attaaatagc tgcctgcgcc cgtggaacaa
    20581 gggctggaat ggtatttttc atgtaaaaag tgcccacaag ccgggtcgcc gacgctgttt
    20641 ttcgctttgc gtacagtagg gtgttgaaat gcgcgcggcg cagccgatgg agacactgat
    20701 gaccaccctt ctgaaatcgc atgtcttgct gggcaccggc ctgctggtgc tggcactgac
    20761 gcccgcctgg gcccagttgc cgaccgtcac cacccggatc gaaacctgga caggaaccgg
    20821 cccgccgccc gactacagcg ccatcgagcg ggcgcagtgg gaacaatggc agctgcagcg
    20881 tgaacaggaa cggcggcaac gtgagctgac gcgccagcag gatgcccaga tccggcagag
    20941 ccaggagagc ctcggcgaac gggcgttgcg ggagcaggcg cagcgcaacg gcacctgggt
    21001 gccccagcac cccggcccga gcgaccagga gcaggccagg cgcgaacagc agcagttgca
    21061 gcgtgaccag tggcggcagc agcgcgagct gacacgccag gatgaccagc ggcagcggca
    21121 aagcgaggag tcggtgggtc agcgcgccct gcgcgagcag gcgcagaacc ggcgcgagca
    21181 aggccgcatc tccagcagcc cccagcgcta gaaacctagg ccgcttgcgg ggcaggcgtt
    21241 gccacaccgc gataacgggt ggcctggtcg tcgagccagg ccccaaaaag cctcagcacc
    21301 gggttctctg acttctgctc ggggtaaatg aagtaataac gccggtcgct gacgaactcg
    21361 tgctcgatca gcggcaccag gcggccctgc ttcagatctt cttcaatcag gaagcgcggg
    21421 atcagcgcca gaccctgccc gtgaatggcc gcctcggtgg tgatggaaaa cagctccatg
    21481 cgcgcaccgg ccaggtcgtt gtcgatgtgc aggcccagcg attcaaacca ctgccgccag
    21541 gcgtaaggcc gcgtgctcat ctgcagcagc ggcaggtgcg ccaggtcggc ggccttcagc
    21601 gtgcgtcgcg tcctgaccag cgccgggctg cccaccgcca ccaggcgttc gtccatcagg
    21661 aaacaggact ccgtgcccgg ccagccggcg tcgcccgcga aaatggccgc gtccagcgcg
    21721 gtatcggcaa acaggaaggg ccgcgtgcgc gacgacaggt gcaccgtgat atcgggatgc
    21781 aggcgctgaa aatcgccgag ccgcggcacc agccacttgg cggcaaaagt cggcaccacg
    21841 ccgagctcca gcgccccgcc ggcgccgccc ttggccatca aggccagcgt gtcgcgctcc
    21901 acgtcgtcga ggcgtgcgcg cacgttgcgc gcgtagttgg tgcccgcttc ggtgagcagc
    21961 acgccgcgtt tggtgcgccg gaacagcttc acactcagga agtcttccag accggcaatc
    22021 tggcggcaca ccgcgctctg ggtgaccgcg tgttcctgcg ccgcctgggt aaaactctgg
    22081 tgccgcgcgg ccgattcaaa cagcgccagc gccgtggtgg aagggatttt tcgacgcatg
    22141 gcgggctcct gcctgggcgg cttggttgga gttccatttt tgcacaaacc cgtgccaaat
    22201 catcggttgc gcccggcggt gaagcaagcg tatgcttgcg ccatcgtcat ccttgcctcg
    22261 gccgcctgag ttgcgatttc tgcacacaga ggcctcaccc accccctttg gagattcccc
    22321 atgagcccca gcccccacaa agccagcttc cactgggccg accccctgct gctcgaccag
    22381 cagctgagcg acgacgaacg catggtgcgt gacaccgccg ccgcctactg ccaggagcaa
    22441 ctggcaccgc gcgtgctgga agccttccgc catgaaaaaa ccgatgtcgc gatctttcgc
    22501 gagatgggct cgctgggcct gctcggcagc acgattcccg aagcctacgg cggcgccggc
    22561 ctgaactacg tctgttacgg tctggccgcg cgcgaggtcg aacgcgtcga ttccggctac
    22621 cgctccatga tgagcgtgca gtcctcgctg gtgatggtgc cgatcaacga gttcggcaac
    22681 gaagccacga aacagaagta cctgccgaaa ctcgcgaccg gcgaatggat tggctgcttc
    22741 ggcctgaccg aacccaacca cggctccgac cccggcagca tgatcacacg cgccaaaaag
    22801 gtggacggcg gctacaagct ctcgggcgcc aagatgtgga tcaccaacag cccgatcgcc
    22861 gacgtgtttg tggtctgggc caaagacgat gaaggcgcca tccgcggctt tgtgctggaa
    22921 aaaggctgga aaggcctgag cgcgcccgcc gtacacggca aggtcggcct gcgcgcctcc
    22981 atcaccggcg aaatcgtcat ggacgaagta ttctgccccg aggaaaacgc cttccccgac
    23041 gtgcgcggtc tcaagggtcc gttcacctgc ctcaactccg cgcgttacgg catcgcctgg
    23101 ggcgcgctgg gcgcggccga ggactgcttc cacaaggcgc gccagtacac gctggaccgc
    23161 aagcagttcg gccgcccgct ggccgccaac cagctgatcc agaaaaaact cgccgacatg
    23221 gaaaccgaaa tcgccctcgg cctgcaaggt tgcctgcgcc tgggccgcat gaaggatgaa
    23281 ggcacggccg ccgttgaagt cacctcgatc atgaaacgca actcctgcgg caaggcgctc
    23341 gacattgccc gcatggcgcg cgacatgctg ggtggcaacg gcatcagcga cgaattcggc
    23401 gtggcgcggc acctggtcaa cctcgaagtg gtcaatacct acgaaggcac gcacgacgtg
    23461 catgcgctga tcctgggccg cgcgatcacc ggcatcgccg cgttttcctg aagccatgac
    23521 aggctccgct gttcgcccgc cgccgctgac cggcgtccgg gtgctggatc tgtcgcgtgt
    23581 gctcgccggc ccctgggccg ggcagctgct cgctgacctg ggtgccgacg tggtgaaggt
    23641 tgaaaaaccg cagggcggtg acgacacgcg cgcctggggc ccgccctacc tgaaggacgg
    23701 ctcgggccgc gacaccaccg aggccgccta ctttctctgc gccaaccgca acaagcgctc
    23761 cgtcgccatc gacatcgcca cacctgcagg ccaggcgcag gtgcgcgcgc tggcgcaaca
    23821 ggccgacgtg gtgctggaga acttcaaggt cggcgggctc aagcgttatg gactggatta
    23881 cgccagcctg agcgccgcca acccgcgcct cgtctattgc tccatcaccg gcttcggcca
    23941 gaccggtcct tacgcggcgc gcgcgggtta cgactttttg atccagggca tgggcgggct
    24001 gatgagcgtg accggccagc cggacggcga gcctggtgcc ggggcacaga aagtcggcgt
    24061 ggcgctgacc gacatcatga ccggcctgta tgccacgatt gccgtgcagg ccgccctggc
    24121 cgagcgcgaa aagtcgggcc tgggacaaca catcgacctc gcgctgctgg acgtgcagat
    24181 tgcctgcctg gccaaccagg cctccaacta cctggcgggt ggactggtgc cgcgccgcat
    24241 gggcaatgca cacccgaaca tcgtgcccta ccaggccttc cccacggccg acggcgacat
    24301 catcctcgct gtcgggaacg acgagcagtt cgccagattc tgcaaggtcg cgggccatcc
    24361 cgaatggtcg gccgatgcgc gcttttccac caacgcgcag cgtgtggcga accgcgcggc
    24421 gctcgtgccc ttgctgcgcc aggccacggt gatgcggact tcggcagact ggatagccgc
    24481 gctggaagcc gccggtgtgc cttgcggccc catcaaccgc atcgatgaag tgtttgccga
    24541 cccgcaggtg caatcgcgcg gcctgcacat caacatgccg catgcgctgg ccgggagcgt
    24601 gccgctggtg gccaatccga tccgcctgtc cggttcgccc gtggcttacc agcgaccgcc
    24661 gcccatgctc ggcgaacaca cggccgaggt gctggctcag tggctgggtg ctcaagagcc
    24721 ggcgtagcac tcgccttgca tcgtcttgtc aatgaaatag gcctccagcc caatactatc
    24781 gggcggaaaa agctatttat ttgatagcag ttcagccaca ccttcgcggc ttcccgccgt
    24841 ggcgcgcagg cgcgatacgg cggccggcag gtctttccat gcaggctgcc ccggcgcata
    24901 ccggctgcgc atgtagctag ccagcgtggc aatctggtcg tcgtccaggc tgtgctggaa
    24961 agccggcatg aagccaatcg ctgccgttgc cggtgcgcga atgccctcca gaatcacacg
    25021 aatcaggttg tcgggtcgct cgctctgcag cttgctgttc agggccagcg gcgtgttgac
    25081 acccagcagc ttgggcccgt ccccgtcgtg gtggcaggag gcacaggcgc cgttgaacag
    25141 gcgctgaccg gggccgggca gcgccgcgcc gctctgctgc gccgccagca ccacctgcct
    25201 ggcgcgttcg gcactctcgg gctcggccag gggctggttg aacgaggtca ggtagctggc
    25261 catggcccgg atgtcatcgt cgggcaaggc gccgagctcc ttgaccaccg gacccatggg
    25321 tccggcggca gcaccatgct gcaggctgta gccgttgcgc aggtagcggt acaactcggc
    25381 ggaagaccag ggcacgggcg cgcgcgacag ggccgtcagc gcgggcgctt cccagccgtc
    25441 gaccaccgca ccggacagga aggcctggcc gccgcgttcg gcgcccagtg cgttgcgcgg
    25501 cgtgtggcag gcaccgcaat ggcccaggcc gttgaccagg taggcgccgc ggttccattc
    25561 cacggtttgt gtcgggtccg tgcggtaagg cgccgggtca tgaaacatcg cattccaggc
    25621 cgccatcagg ggacgcacat tgaagggaaa agcgagctgc gtggcgggtg tctccgagcg
    25681 cacggcaggc tgcgccatca ggtaagcgta cagcgcggtt aaatcttcat cactcgtttt
    25741 ggcaaaggcc gtgtaaggga aggccgggta caaatggtgc ccgtcacgcg agataccttc
    25801 acgcatggcg cgctggaaag cgctgaagga ccacaggccg atgccggtct gcgcatcggg
    25861 cgtgaggttg gtggtgaata tcttgccgaa gggcgtctcc atcgcgcggc cgccggcatt
    25921 cggcgtgccg ccgggcgcgg tgtggcacac cacacagtca ccggccgcag cgagctgccg
    25981 gccgcgctca atcgtcgccg cggtgtagat cgaagaactg ctggacaaca ccggtgcaat
    26041 tgcgggccgc cagcccagca gggcagccgc cagaccaaac acgccggcca gcatggcacc
    26101 ggtgcgggac cagcggccca ggccctgggg ccagggcgcg ttgacgggcg ggcgggccaa
    26161 cgggggcggc gcggccaggg caggcgacac ggccggcacc tgatcgggcg ccggttgcag
    26221 cgctgcacgc accacctccg cggtgaaagg cggctggcga aaccgcacgc cggtggcatc
    26281 aaaaatggca ttggcaatgg cggccgtccc gggcaccgag gccgactcgc ccgcgcccag
    26341 cggcggctcg cccgggcgcg ccatggttac cacctcgatc accggcacat ccctgaaact
    26401 caggatgggg taactgcccc actccaggct ggccacggtg ttggttgtcg ggttcaggct
    26461 gacctgctcg gtcaacgccc ggctggtggt ctgcaccacg ttgccgtgga tctggtgctg
    26521 cacaccggcg gggttgatca gcgtgccggc gtcatggccc accaccacac gccggacctg
    26581 cacttcgccg gtcttgcggt tgacctcgac gtcggccacc caggcggccc aggccgcgcc
    26641 ataacccggc cacttgctgt ggacataacg cgcataagcc acgccctgtc cgcgcacgat
    26701 gtcggatgcc ccgctttccc cttcagcctg ctgcggctgt gtgtggactt tccagccggc
    26761 acggtcggcc gtggcgcgca gcagttccgc cgcacgtggc tcgctcaggt aacgcaggcg
    26821 gaattcgacc gggtccacgc cggccgcggt ggccagctcg tcgatgtagg actcgtgggc
    26881 aaaggaattg ggcagcgccg acacaccgcg cagccacgaa gcgcgcaaca tgggcggcat
    26941 gtcgttgacc gacacgcgaa ggttctcgta gtcgtaaggc gggcgagcac tgcggtcgcc
    27001 catttcatag gcgcgtgcca ccggctcgac ggtgcgcgtg agcagcagcg ccagtgtcgg
    27061 tgcaccgttg gacgggtagg aggtttgaaa atcgtaggcg gcgaccgagc cgtcggcatt
    27121 gagcccgccg ttgacctgca tgagctgggc cgcgcccttg ggctcccagg cgtgttcctg
    27181 ctcgcgcgtg agctgcaccc gcaccggcgc accgacagcg cgcgagagca aagccgcatc
    27241 ggccgccaca tcgtcggcgc agttgcggcc gtaacagccg gccgcctcca tgcggatcac
    27301 gtcgatggcc gtgtccgcca gccccatgag gcgtgacagg tcggcacgca gcacatgcgg
    27361 gttctggcta ccggcccaca ccgtcatgcg ctgttcctgc cagtccgcca gtgcgcaaga
    27421 tggaccgatg gagccgtgca tctggtaagg ccagacatag ttgcgcggca aacgctgggc
    27481 cgcgcccgcc atcgcggcat ccacgtcgcc ctcgtttatc agcttgcgct gcgtggccgg
    27541 gttggcgcga atggcgtttt ccagatcgtc aagattggtc agccccggcc agggcttcca
    27601 ctggacacgc agctcacgca gcgcctgctc ggcatgctcc tcgcgctcgg cgacgatgcc
    27661 gatgaaatcg cggatgacca ccaccgcacg aatgcccggg atatgcgcaa tcgaggactc
    27721 atcgaccgat tccagcgtgt tgccgatgaa atcgccatgg tcggcgccgg cataaggcgg
    27781 gcgcacaacg cggccgtgca gcatgcccgg cacgcgcatg tcgtgcacaa aggtcagtgc
    27841 gccggtggcc ttggcaggaa tgtcgacgcg cgccgcgctg gtgccgacga tgcggtagtc
    27901 ctccggggat ttcagcggcg aatccagcgc cagcgaaagt tcaacatgct gttgtgccac
    27961 cagggcaccg tagcccaccg accgggcgtc gctgccgatc accgacacca ggccatcacg
    28021 caccgtcagc agcgccctgt ccacctgcag cacgcccgcg gcccggtcca ccagccaggc
    28081 gcgcgcctgg gccgccgccg cccgtagcgg gacggcatga atctgtatcg atgcgctggc
    28141 aatggtcgcg ccctgattcg gcgcgcgggt ggtatcgccc agcaccatgt tgacgctggc
    28201 cagcgcaaca tcgagctctt ccgccacaat ctgtcccagc gccgtgcgga tgccggtgcc
    28261 caggtccaca tggccgttca gggccgtcac actgccgtca tcccagaccg ccagcaatat
    28321 ctccacccct tcgagtggat tgccgctgac cagcgcgggc tggcccgggg cgggcggcgg
    28381 cgcaggtggc ggttcgcgcg tgaccagcag cacaccgccg gccgcatgaa agtcggcacg
    28441 tgtgaggggg gtgtcggcgc ggcgcgtcat ggccgggggt ctttgggaca ggcttgccgc
    28501 gtgagttctg ccgcacgcag caccgcattc acgatctcga gatgcgtgcc gcagcgacac
    28561 aagttgccgg acaactcgcg gcgtatctcg tcctgcgtcg gatccggctt gcgcgccagc
    28621 aaggcggtgg tcatcatgat catgccgctc aggcagtaac cgcactgggc cgcctgctct
    28681 tcgatgaaag cccgctgcac cggatgcagc gccgccaggg acccgagacc ttccagcgtg
    28741 gtgatctcgc ggccggccac gccgccggcc gggatcacgc aggcgcgtgc ggccacgcca
    28801 tcgaccagca ccgtgcaggc cccgcattcg cccagaccac agccgtattt ggggctgttg
    28861 agttcgaggt cgttgcgcag cactcccaac agggaggcct gcggcgagac cgtcagcgtc
    28921 cgcgtcaagc ggttgacgct gaaggtcacg acctgcgagc tcatcgtggg caagtcaggt
    28981 cgcgcttcag gccaggtctt tggactggat gcgtttgacg cccttggccg ccatctgctt
    29041 ccaggccgcc gccagcgagc cgttcaggtc aatggcgcgc gtggcatcct cgatcacata
    29101 gacctcgaag cccgccttgc gcgcgtccat cgcggtccag gcgacgcaga agtcggtcgc
    29161 cagcccggtc acaaacacgc gcttgatgcc gcgctgcttg aggtagccgc ccaggccggt
    29221 cggcgtcttg ccgtcggcct cggtgaaagc cgaataactg tccacttcct ggtggtagcc
    29281 cttgcgcaca atgacctggg ccgtgggcag cgccaggtcc ttgtgcaggg ccgcgtcgtc
    29341 cgtcccctgc acgcagtggt caggccacag cacctggttg ccataagcca gttttttgac
    29401 ttcgaagggt ttcttgcccg catgggtgct ggcaaaggac gcatggccct tggtgtgcca
    29461 gtcctgcgtg atcacgatgt tctcgaaagc gccggcgagt ttgttgatga ccggcaccac
    29521 ctcttcgccg cccttgaccg gcagcgtgcc cccggtcaca aaacagttct gcacatcgac
    29581 aacaatcagc gcgcttttgg cgtcgggctt gatgccggcg gcaaaggcca cgcggccagc
    29641 caggccggca gcagcgacga ggccggcgcc ggacagggtt ttcaacaggg ttctgcgggt
    29701 agcgggatgc atgatgaaat ctccttaaac gggttgaatg acgttgacaa caactggcgc
    29761 actaaacgct gaggtaggcg cgccgcacct cttcgttggc cgacagctcg gccatggagc
    29821 cctcgtaacg gatctggccc ttttcgagca cataggcccg gtccgacacc agttccgcaa
    29881 aatgcatgtt ctgttcggac agcaaaatgc tgacgccctg cgacttgagt tccaggatca
    29941 tgtgtgccat ctgctcgaca atcaccggtg ccacaccttc cgagggctcg tcgagcagga
    30001 ccaggtaggg gttgcccatc agggtccggg cgacggtcag catctgctgt tcgccgccac
    30061 tcatgcggcc cccctggcgg tccggcatct cgccgaggtt gggaaacaac ttgaacagcc
    30121 gctcgggcgt ccagaccggc gcctcgctgc cgtcgggcca gcggcgcggc ggctgccgcc
    30181 ccacttccag gttctccatc accgtcaggt cggtgaagat gcgcctgtct tccggcacaa
    30241 agcccaggcc catacgcgcc gcgtggtggg gttcgctttt tgaaatgtcg gtccccagga
    30301 aggaaacact gccgcgccgc ttgtccagca tgccgatcag cgttttgagc gtcgttgatt
    30361 ttccggcgcc gttgcgcccc atcagggcca ccacttcacc gcgccgcacc tcgatgccca
    30421 cgtcgtagag gatctgggca gcgccatacc aggcgcacag gccctgggcc tgcagcagga
    30481 cttcgttcgc agcgctcatg aggcggcatc cttttccagt gtctttttct cgaaggtctt
    30541 gccgctgccg aaatagacct cctggacctt gggatggtcg cggatttcca gcggcttgcc
    30601 ttcggcgatc agccgccccc gcgccagcac gatcatgcgg tcggcgtagg caaacaccac
    30661 atccatgctg tgctccgtga acagcaccgc catgccccga tcgacaacca gttgtttggt
    30721 cagggccatc aggtcgttgc gctccttggg cgccatgccg gccgtaggtt cgtccatcag
    30781 cagcagcttg ggattgttgg ccatggccat ggcgagctcc acacgcttga cgtcgccata
    30841 ggccagcacg ctgcagggcc ggtcggcctg gggcttcata ttgacctggt cgagcagcac
    30901 cagcgcctcc tcgcgcttgt ggtcggcggc gcggcgccac atcgaaaaca gcttgcggtc
    30961 cgatgacagc agcgccatct ggacgttttc caccaccgtc agcgaggaaa aggtctctgc
    31021 aatctggaag gtccggctca cgccaagccg ccagatgtcg cgcggagcca ggccaatcag
    31081 ttcctggccg cccagctgga tcgaaccggc atcggcgcgc agctggccgt tgaccatgtt
    31141 gaaggtggtc gacttgccgg cgccgttggg cccgatcagc gccagcaact cgccggcggc
    31201 cagctcgaag ctgatgccgt caaccgcctt gacgccaccg aatgatttgc cgaggccggc
    31261 tactttgagc aagctcatga ctgcccctcc ttcgcgccgc gcaggcgcga cagcagttgc
    31321 tggacaaagc cggcaatccc ttgcggaaac agcagcacca gaatcaggat gatggcgccc
    31381 agcatggcgc gccagtagtc ggtgttgcgc gctaccgtgt cgtgcagcca ggtgaaggtg
    31441 acggcaccga cgatgggccc caccagcgtc tgcacaccac ccagcagcac catgaccaga
    31501 ccgtcaatgg atttgctcac atgcaggctt tccggggaaa tgctgccttt ggagaacgca
    31561 tacagcgcac cggccaggcc cgcgaaggtg ccggcaatga caaaagccac ccattgcatg
    31621 cgtttgacgt cgatgccgat cgcgtcggag cgaagcaccg aatcgcgtgc agcacgcatg
    31681 gcgtaaccaa agggtgaata cagcatgcgg tgcatcatca gcaccgcccc ggccaccagc
    31741 gccagggtca ggtagtagta catcttcttg tcggccagcc attcggacgg ccagacgccg
    31801 gtcagcccgt tgctgccgcc ggtgaaggca tcccactggt agcagattgc ccaggtgatc
    31861 tgggcaaacg ccagcgtcag catggccagg tagacgcccg acagccgcac gcagaaccag
    31921 ccgtagatga aagcgcccag cgccgccagc gcaggcgcga caaccagcgc cacctccatc
    31981 ggcaggttca ggctgcgcac cagcagggcc gcgccgtagg cccccaggcc gaagtaggcc
    32041 gcatggccaa aggaatgcat gccggccggc cccatgatga agtgcaggct ggccgcaaac
    32101 agcacggcca ccagcaggtc aatcagcagg accgtcacat acggcgactg ggcggtcagc
    32161 gcaggcatca ggaccagcag ggccagcagg gcggcgccgc cgaacttgta gaggcgatcg
    32221 ggcgggcgca gcggcgcctc ggcggtgccg gcatggcggc tggtcgcctg gggccggccg
    32281 aacaggcccc agggacgggc caccagcacg acggccatca cgagaaactc caccaccagg
    32341 gtgagtttgg aaaacgaaat gctgacgccc cagatttcca ccagaccgag ccagatgcaa
    32401 atggccttca gctccgcaat cagcaatgcc gccacatagg cgccggggat ggagcccatg
    32461 ccgccgacca ccaccaccac aaaggcggcg ccgatgatgt tgaggtccat ctcgaggttg
    32521 gccggttcgc gcggcagctg cagtgcaccg cccaggcccg ccagcagtgc acccagcgca
    32581 aacaccgcgg tgaacagcca ggcctggttg accccgagcg cgctgaccat ctcccggtcc
    32641 tgcgtggcgg cacgcaccag cgtgccccag cgggttttgg tcagcagcag ccagagcagg
    32701 ccgagaacca ccggaccggc aacaatcagg aacaggtcat aggaaggaaa ttgccggcca
    32761 aggatggaca ccgaacctgt caggccgggc gcccgcgggc cgagcaattc ttcaggcccc
    32821 cagaaccaca gcaccccgtc cttgatcacc aggcccagcg caaaggtggc cagcagctga
    32881 aacagttcgg gcgcccggta gatgcggcgc agcagcagga cttcgaccag cgcaccaagc
    32941 acaccgacgg ccaacgcagc cagcagcagg gccggccaga agccgaccgt gccgcccagg
    33001 gtggccacca gcgaataggc cacataaatg ccgaccatga aaaatgagcc gtgcgcgaaa
    33061 ttgacgatgc gcgtcacgcc aaagatcagc gacagcccgg ccgccaccag aaacagcgac
    33121 gacgcaccgg ccagaccatt gagcaactgg acgacaaagc ctgaaaagct catgaattac
    33181 ctttgtttta cgggagacag ccccttgcag gcggcacgac aggcctgggc ctccgtgcag
    33241 gagtccatcg ccggcgaaac cttcggccgg cacccgcgcc aatggcgcgg gaccgaacct
    33301 ggtgtcgccg tgcgccagcc tgctcagtcg gcggggcgca gcttcttgat ttctgcagct
    33361 gacggctgga atttcgcgcc gtccatgtag cggtagtcca ccatcacacc cttgccgccc
    33421 tcgttcttgg tgcgcccgac gtaggcgccc atggtggact ggtgatcttc aggacggtag
    33481 accacgggac caaaaggcgt gctgaccttc aggccgctga aggctgcgat cattttttcg
    33541 gtatccgtgg acttggcttt cttcatacct tcggccagcg acttgatggc gctgtaaccc
    33601 actacggaac cgagacgcgg gtagtccttg tacttgccgt ggtaagccag aaagaaggcc
    33661 ttgtgctcgg gcgtctggat gccgtaccac gggtaaccgg tgaccagcca gccgttgggc
    33721 gtctcgtcct tgagcggatc aaggtactcg ggttcaccgg tcagcatgct gacaacttcg
    33781 cggccctgga acaggccgcg ggtgttgccc tcgcgcacaa acttggacag gtcggcaccg
    33841 aaaagcacat tgaagatggc gtccggcttg gcatcggcca gggcctgcac cacgctgccg
    33901 gagtccacct tgcccagagg cggcgcctgt tcggcgacga actccacatc gggctgtgcc
    33961 gctttcagca gcttcttgaa gctggcggcc gcggactggc cgtattcata gttgggataa
    34021 accacagccc agcgcttttt cttcatcgcg gccgcatcgg gcaccagcat ggccacctgc
    34081 atgtaggtgg aagcgcgcag gcggtaggtg tagcggttgc cgttttgcca gacgatcttg
    34141 tcggtcagcg gctctgcggc aagaaagaaa aatttcttct gtttcgcgaa gtccgtcagt
    34201 gccaggccga tgtgcgagag aaaggagccg gtcagcacgt ccaccttctc gcgcgaaacc
    34261 agttcctcgg cagcacggac cgcgtcaccg gggttggcgt tgtcgtcacg gacaatcaac
    34321 tcgacctttt tgccattgat gccgccgccg gcattgattt cctcgacagc cagttccatc
    34381 cccttcttgt agggctcgag aaaggcgggc tgggccttgt agctgttgat ctcgccgatc
    34441 ttgatgaccc cctgcgcgtg ggcggccccc agcgaggcca tcagacccag aaaagtcatg
    34501 gtgcccagca tcttgtattg catcttcata aaaaaactcc tgcttgtcgt ggatcggaat
    34561 ggttggtgag gagcgccgct taccggagcc catcctcgcc cttgatgtcg tggacttgca
    34621 agccgcccac gcgcggtagt ggtcggccgc tgtctgtgac gacgactgca accacaattt
    34681 cattggcacg cggtgcatcg gcgacacgcg cctccatggc gtcaaaatgg ctgcgcacaa
    34741 aagccgcgtc cttgtgtccg agcggaacgt cgatcgcggt accgaggccg ccccgcttct
    34801 tgctggacgg cacaagggcc gcgccctttt cgactgccag acgcaagggc gcacccagtt
    34861 tgggatgcag gatggctgcg gcatgctcca gttctccggc ctcgccgaca atcgccgcct
    34921 tgccgtaact ttgcgcctgg ccaggcgaaa tgcccagcgc ctgcacacat ttggcgccca
    34981 gcaggccgcc cagctcttcc ccgatggcga tcaactcgtc gaggttctcg acgtacttgc
    35041 cggcaaacgg attggcgatc accgccatgg ccagcgcccg ccgtgtcggc ggattgactg
    35101 cttgtcccat ttccatccgg gtctcgtcga cctggacaat gatttttctg attgtggctg
    35161 tcatgttgtg gtccttgcgt gttgtcgttg tgtcgatggc gcctcagcgc aggccgtccc
    35221 acttgctgat ggcctgggct gccagcccac ctacccgcgc atggacacgg ggaccggtac
    35281 tcatggcaag aataaagaca atttcgtccg gcaggggggc gccgggaacg cgaacttcaa
    35341 tggcatcgaa atgactgcgc acgtaggcgg cattgatgtg tgtcatggga acgtccagcg
    35401 cggtaccggg tgcacccact ttcttggtgg aaggcacgat ggagagggca ttgccagggc
    35461 gtccggattt ttcctcgccc tgccctttgg cataagccgc agcatcaccc ttccagccca
    35521 gcagttcccg cattgcgtaa ccgccaggga catgccagag cgcaccgtgc tcgagctcgc
    35581 ccgccgcacc gacgatcgcc cccttgccat aaccctcgat acggtccgcc ggcacgtcca
    35641 tcgccgcgcg caggcgttcg gccatgtcga taccgacggg ttgaagcgcc tccatcatgg
    35701 gcaggatgtc gggctcgtag cgccctgcaa acggattggt cagcacggca gcaatggcgc
    35761 cgcgcatcag cggcaccggg gcgaccgggc cgaactcgtg ccagatctgc tccacgtgcg
    35821 tgaagatgcg tcgtatttca atcatcggtt tgcgtccatc catttcatta agcaaatact
    35881 gaaccaactt gcacgccccg gctgtcagca tgccggtttt ccagggcaag cgtgtccacc
    35941 gcaccggtgg tcatcacctg attctggcaa accagcgcgg ccgaccagat caggcccgca
    36001 gcgcgcaagg cctgcgcgcg cttgaggccc gcccgcaagg cctgctgcac cagcgccggc
    36061 tccagcggcg gcacatcgac tgtgacggga atggcgccga ggtcgcaatc atccttcaat
    36121 tcgcaggccg gcctgcgccg gatgcgggta tcgcgcagat cgaccgcatt ggcaatcacg
    36181 gtggccgccg catcggcctc ggacgcggtg cgtgcgagaa ccgtgacgct gtcggcgatg
    36241 ccgagtgaaa aactgcggcc gcgccagccg ctggtggcca cgccgcgcac gggcagcaca
    36301 ctggccacct cgaactggcc atcggtctgc accccctcaa gaatttcgtc catgctcagc
    36361 cgcgaaaggt cggcgtaaag acctatgcgc accgactggg aaggcgtgag atgcagggcg
    36421 atgtcgccgc cattgttgac ccaggcacgc gtcacgccgc tgacggcgta acaggccacc
    36481 agctcctgcg ccacggcgcc ggccaccgcg gccatcgggg tgatgaaact gcttttatat
    36541 ggctgacagg catgccacat gcgcctggca accgggcccc gcaccgggca ttcgccgcgc
    36601 acgggaagcc gcaggaagac cagctcctgc accagttcat ccaggatgcc gcgaaaacgc
    36661 agccaggcat cggtgtgcgc cagctccagc gcctcagcac tgccgcttgc cccaatgacg
    36721 atgtccatcg gaccgtgctg gaaatgccag cggccgtcgg gcagtagcgt gcgctgggca
    36781 tccatgcgtc gcctgcctca acccagcaat ggcggccgcg cgagcggcca tggattggcg
    36841 gcgggcaagg gcacccactt acgggacgtg ggcgcacctt cgttgtgcca agcaccccgg
    36901 cgcagtgcat cctccagcgt gcgcacatgg tccatgtgac cgcccagcgc acggtaatcg
    36961 tcgagcttca tgctgaattc aatgggtgcg acgatggccg gtgtgggcac ggtgccgaag
    37021 ctgttgtccg gcatgcgcat gacgtcggcc atcacagtga tcccgccgcc cggccagata
    37081 taggcgggcg ccccgccgca ggtgacattg accagcgcgt ccttgatggc acgcgtcagc
    37141 aaaaccgggt tttccgtgac cccggcacgc aggctgccgc cggcgccacc caggaaaagc
    37201 acagtcgtca gcgaaggctc gcagttttcg ccgatgcgct cgacaatgcg gcgcacctct
    37261 tcgggcatgt cctgctccac cggctggagt ttgtcgtcaa ggacatacca gctcgcgtgc
    37321 tcgccggtgg tcgatgtcat gagcagtcgc aggccgggcc aggccgcctc ggcatcccag
    37381 ctttcaatga tggccagcgg atcggcgatg tccgtgccgc cccaccctgt tccggggttg
    37441 gccacctgga aataacgtcc cggcgtggat ttgcggcccc gcatcctgat tcccgaggga
    37501 cgcatgtcca ggcagcggcc ggcctggtgc tcggtcagca caccggtgat gtgatcgtcg
    37561 accaccacca cctcgtcaag cttgccgaaa aactgcttcg caaaaatgcc gatggtggcc
    37621 gagccacagc ccacacgcat gcgcttttct tccaccccgt tgacgatggg tgcgcggccg
    37681 gcctggatca ggatctggga accgccgtca atcgtgagtt cggccggctg cttgttgccc
    37741 agcgccatca tcatctcgac cgtcacgcga ccctctttct tgctgcctcc ggtcaggtga
    37801 tgcacgccac cgagcgacaa catctgggag ccatactccg ccgtggtcac atggcccacg
    37861 acttcgccct tgcagcgcac attggcctgc tctgcgccga ggaagcggtc ggtatctatc
    37921 ttcaccttga ggctgcagta gctgaagatg ccttcggtga ccaccgtcac catgtcaacg
    37981 ccagccgccc tggacgacac gatgaaaggc gccggtttgt agtcggggta ggtcgtcgag
    38041 gagcccacgc cggtgacgaa cacctcgtcg gccagcagca ggtcaccgct ccaggatgtt
    38101 tcgtccgggg aggcctccgg caatgcggcc ggatcaggca tgcccgcgac ggcggtacgg
    38161 gcagtgaacg gcacgagcac aggctcgcca ccgtccaggg tgcggcgcag caacagcacg
    38221 ggatcaacac gaaccagcgt accgccctgg ttggcatacc ggtcgcaggc gccgctgcgg
    38281 ccgtcggaaa tctggcacaa caccggacag gcgttgcatt caatcttctc gctgttcatc
    38341 cgctcgctgc gcggccgggc cttttccagg acctggggaa gccccgggga atccatgcct
    38401 ggtaatccgt ccgtttttgt atgctcggtc atcgtgtttg ccttcgtcct gccggtcatc
    38461 ccgcgcgcca ctccagatgt agcgcttgct caactttaat gttgcacttg ctacatttga
    38521 aatcaatgta gcgcatacgc atggccggtg caatagtgtt tacacgtcaa atccggaatt
    38581 tctcaaaaaa atgttatggg cgctacattt aaatgttgcg ttcactacat ttgtatggca
    38641 cattgatgcc atcgaccgcc ggcggccttc ccggcagacc ccctcaagga cctcatgacc
    38701 cacgtcgtca ccgatgcctg catcaaatgc aaatacacgg attgtgtgga cgtctgtccg
    38761 gtcgactgtt tcaaggaggg gcccgacttt ctggtgatcg atcccgacga gtgtattgac
    38821 tgcgccgtgt gtattcccga gtgtccggtc aacgccattt acgccgacgc ggacacgccc
    38881 ccgcagttcg aacccttttt cgagctcaac cagcgcctcg ccagacaatg gcccaccatt
    38941 tccaggcgca ttgcgcccct ggcgacggcg gagcaatgga aagacaaaac cggtaagctg
    39001 gcgagcatcc atctcgacgg ctgaaccact caccccgaac ctccagcccc tacattcacc
    39061 acaatgagcg ccttcaacga agaacgagtc ctgtccgtcc accactggac cgaccgcctg
    39121 tttaccttca ccaccacccg cgaccagtcg ctgcgcttca gcaacggcca tttcacgatg
    39181 ataggcctgc gggtcaacga caagccgctg ctgcgggcct acagcatcgt gagccccaac
    39241 cacgaggaac atctggagtt tctgagcatc aaggtgcccg acggccccct gacgtcgcgg
    39301 cttcagcaca tcaaggtggg cgacaccatc atcgtcgggc gcaaaccgac cggcaccctg
    39361 gtgatcgact acctgcttcc cggcaaacgg ctgtacctgc tgtccaccgg aaccggtttg
    39421 gcgcccttca tgagcatcat ccgcgacccg gcaacttacg aacagttcga gcagattgtt
    39481 ctggtgcatg gcgtgcggca ggccgacgag ctggcctacc acgacctggt cgtggaccat
    39541 ctgccgcagc acgaatttct cggcgagatg gtgtccaggc agctgctgta ttacccgacc
    39601 gtgacgcgcg agagctaccg caacatgggc cgcgtcaccg acctgatgga aaccggcaag
    39661 atgtttgaag acctgaagtt gcccccgctg gaccccgcgc aggaccgcgt catgatctgc
    39721 ggcagcccgg gcatgctcaa ggacctgaaa cacatgctcg agaaacgcgg ctttgtggag
    39781 ggcaacacct ccagacccgg cgactttgtg atcgagcgtg cgtttgccga gcaataatat
    39841 ggcccccacg cttctcgctt cgcgtagtac gctgcccccc gagggggcgc tgcgcct
//
LOCUS       DAIDFG010000002        37655 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_2_length_37655_cov_18.759043, whole genome shotgun sequence.
ACCESSION   DAIDFG010000002 DAIDFG010000000
VERSION     DAIDFG010000002.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 37655)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37655)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..37655
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2_length_37655_cov_18.759043"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         7213 a        11514 c        12068 g         6860 t
ORIGIN      
        1 ggtcactgaa tggaaaatcc acgtccgcag tggtcagtcg ctccagttgg gcggcggcca
       61 gattgctgag gtcgaggtag cgatccgcct gctcgtcggt caggcccatg ttgcgcagca
      121 ggtggtactg cttgaggtaa ttctcatgct ccaggacggc gctttcccaa agccagggtg
      181 agacgttcgg gttggacggt gctgggcgca tggctggatt atcctggagc ttctacaaag
      241 gttttttcag cacacggtac atgtgccggc gggtgatctt gttgtcgcgg cgcaccacgt
      301 cgaaatcctc accggcatcg atgcggcgga tgatctcgcg gtcgcgatga gcgcgcagag
      361 cgacagcgca ggtgggcacg tagagcggtg tgccggcata gcgttgcacc aggcgtgttg
      421 cagcgtcagg accgacggca tccaccagca tggcccacac ccggctggtg ccgttgatca
      481 cttccgggat cttgagttcg tcgccggcgc acattttcac caggtcaatc gtcgcgtcga
      541 ggccgatcac ctgcaccagc tcgcgcgcag ttttcggcaa gtcgtcttga gcgacttccg
      601 agcgcagcgg catggttaga tcgaacaggc tgaactgttc gcgtggcggg tcgatctggc
      661 gacgcggttt cattgcgtgg gcactccctt ttttcgttgt tggacgccca gcgccgccga
      721 gaccgcatgc agttgctcgg gcgtgcacca ttcgtaaaac tgcacgccga acatctgctc
      781 ggcaatgccg tccgcgtagg tgtctggcag gcggtcgagg ctgatcagct gagcgcgtat
      841 ccggcgaacc agcttgagct tgtccggcga gggtgttggc cgcgaggggc gcttgccgtg
      901 gctggccgtc ttcggcttcc agcccagcgt ggccttgaag taatcaagga cgcgctgccg
      961 gccttcatag tcgaggtttt tcgagctgtc ctgtccaccc ttggcaacca ggatggcccg
     1021 gtagtcgtcg tcgctcagct tcagctcctt cctggcgatc tggatcttga tgacgtcgct
     1081 gttgcggtgg tcgcgggcta gcttgctcat aatggctgct ccatcaaccc aaacctgcga
     1141 ccaacaccat gcactggtac atctacgaac tcggcccgat cgatcattac tggcagcaca
     1201 tgaagactgt tgttgagacg gcaaaagagc tgggcgcggc ctcggcagcc gcccaaattg
     1261 ctggctttcc tgaagacact gacggggaaa gcgtgagcgt gagtgagttc cttgccaaat
     1321 ggaaggacgc cagcagtaga gtgacctcta gctccgatga attgcggctt ccgccagtgg
     1381 ttttttgggt gccgtgggag ggatacttta aatacggctt cgtgatcaag cacgacaaca
     1441 acggcactac ctacgttgtg tcgccggttg agctgccgca tcttggtgag gccagctagc
     1501 ggtagtttgg tcatgattcg acaatctcaa aatttgcgtg gaagttagcg ttcccgtaga
     1561 cgcgagggct gtgacggcta ccttcaaaaa cgacccaatc accagggttg attttttgaa
     1621 agccatgcga ggcgctgtgc acgccccagg tggacattcc ggtaacgcca gagctaacga
     1681 cgatgtcggg cagatcaccg ggctgatgcc actggcaggc atcaagatcg accgggcgtg
     1741 tgcgaatggc gatgaaggtc atcagtcagg ccctcccagg agcatctggc cggctgcgtt
     1801 ggtgtcggct cgcctcgcct tgatctgcga cgccttgacc aaggacagct ccacgctcgg
     1861 ctgctcaccg aaagcgtagg tgtagccgtg atcgccgtaa tcttttcggg ctgcaaaagc
     1921 cgactgcagc agctcgacca gcttcatgcc cttgtcggca ggcatgaggt agtcgttgta
     1981 ggcgatgctg acgcagcaca tcgccacagg cttcttcgtg acaggtgcgc ggctcatagc
     2041 ccgattgcct ttctcacaag ctgttcatag tggtgatcaa cggctggcac gatgatccag
     2101 gtgtagatgc cgaccagcga agtgatggag aagccgatct gttgcacgaa catgaactga
     2161 tggccgtgcc cgttggcgaa ggacagccag ccgatgttgc tggcggcgaa gaagagccaa
     2221 cccagggggg cctgccggcc tttcagggcc agcagcaagc tgccaaacag accgcaggcc
     2281 gtggcgaaca gctctatcac ggcgaacggc ctgccgaggt attgcatcgc cttggtgagc
     2341 cacggctgcg cggcctcgaa aaggtcaagc cagtggctca tgtctgaatc ctccaaggct
     2401 caagagcgat aaacagacca tgcccttcaa tggaggcatt gaggccgtcg cggcttgggt
     2461 gcgggtcaaa caccaaccga ccatcttcgc caacgcatgc gtggccgaca tcggtgaatc
     2521 ttgtggtgcc tccatagatc tcgtgatgca cgccacgaat tccaaagtcg gcaagcatct
     2581 ggtgaaagcc ctcttgaggg aaagaaagat aggccagacc acggggacgc agccaggcgt
     2641 tcatgtcctt cagccatgtg tctttgctcg aaaacacagg gatggtctca atggggatat
     2701 gcagcagcga cgccagcacg gccgacaggc aatttccgtg cttgccgttc gcgggatcat
     2761 gaagttgagt ctgggttaca ggtatcacag cggtagctcc aggttgtttg ccttggcctt
     2821 gcgggtcatg taggcattga tggtgtggtg gtcctggtcg taggccaggt gatggcgctg
     2881 gcaccaggcc gcgaggttgg ccgggtcgca gtgttccggg atgtggtcgc ggtgagcgat
     2941 ggtcagcacg atctcgatca ccttgaagcc gtcagccagg tcagcggcct cgacgtccat
     3001 gctggccgct tcatccttca tgcagatctg cgtgaagttc ggtccttccc agtagccgac
     3061 cgcgtgattg accgcgcagc agccgggcca ctcgcagcgg ttgccggccc gcaggaggat
     3121 ggcagcgcgg atctccgtcc agtcggcggg atagcgggcg cggttttcag gtttgattgg
     3181 catcgcagca gctcacgcag tgagcctccc gtagaccacc aggagccaag cggcgacaag
     3241 gatgctggca acgatccgac gcgcctcgcg cttgagcctc tcgcgccagg cctgaaagat
     3301 caagatggtt gtgaccagca cgggcagcac cagccagggc tcatagcgcc cgtacaacac
     3361 caatacccag cacaccaggc acaagacgat ggcgatcagc gagcaatcgt agtcaagggc
     3421 agagccgatg gcggcgaggt ctatccaaag gccggcagcc aacaccgcga ggcagagcgc
     3481 cagcggcagc atcgtccaga acggaacggt gatggtgaat gtcatgctgc gctcccctga
     3541 cggttgctaa agacaacgcc gcagctcgtt tctttgccgt tccctttggc gcaccagtcc
     3601 acccacgcga tgagcgcgtc tacttgtgcg gtttcgttct ccgcctctgg cacgcctggt
     3661 accaacagct tgcctgcact cttgcccatg ccttcgcggg ccagcgcact gacccgggcg
     3721 cgcagcgggc ggtccatgcc cgtggcaaac gacagggcgc cctcgggcac ttcgcggccg
     3781 aactcgatca ggccggactg ccaggcccag gcgtgcaccg gctcggcgtg caggcgccag
     3841 tgggtgatgt tgtgatcgct gccgcgcggc aacggttcaa cggtgaccag ggatgcgccg
     3901 tagagcttct cgcccaggcg ctcggccgcc tgcttttcac cagcggtgca gcttgcattg
     3961 aagccgcgca cgatgttggt ctggtaggtg ccgcctgttg agcgcaccgt gatgtcttgg
     4021 gtttggactt cggttttcat gctgcaccgc ctttgccgct ggcagcgcga gtgatggcgc
     4081 ggtcgatgga ctcacatcca gccacagcca aggcggcaac ctggacaagg cgatgacggg
     4141 ctttggcgag actgttcatt ccagccatga cacgcgccca gcccgtgtag ccttcaatca
     4201 acaggcagaa ggtgtgcgta tcgtggccgt cgtcatgttc tggaccgccc cactttgcgt
     4261 cctgtcgttg gcgctccgac tccacgtcgc acaaaactac ccgcaaagaa tccggcgctg
     4321 gatcgcgggc ctcgaccagg gcgcgcaaca gatcgaccgg gatgcccgcc gtgttgggtg
     4381 tgacggcatc gttattcatc ggatcgatga aggtctcggc gaacggttcg ccgcgccggg
     4441 cttcggcggc cttgaagtgc tcagcgatgg cctgggcggc agcgacaaat ttggtgttca
     4501 taaattggcc tttaaagcgg tttaaaaagg ccccaaggcc gaagccctgg ggcggtgaag
     4561 atcagccggt tgcggtttcc aggccgctgg cgtgatcaag gaaacatcag tcgaaacggg
     4621 tgaagatcca ggacaggaag ccgatcaaca cgatcagcgc caggctttcg atcaatgcag
     4681 cgtccatgtg cgtcctttca gatatcggtt gggccgatct gcagcagctc gcgctggctg
     4741 gcatcacggc tttgaactgt ctggtcagcc tgctgcgcgc gccaggccgt gagcgccttg
     4801 gggcagttgc tgcagacgcc ggtgccgtcg cggtgattga cgccgagcca gctgcagccg
     4861 ccggggcagg cgcgcagttc ggtgcatccg cagttggtgc aggtggtggc gttcatacgt
     4921 ccctccggtc ggtgtgcttg aactcatgcg tgtcacacac actggtgcgg ccgaccggca
     4981 tggtgtgacg cctgcagcgg ctgttcgaag tctcgaaata gacgcccttc tcaccctcgc
     5041 ggtcatcgtg gtcgcagagg tagaagctgc agtttttgca ggctggctgc ggcaacggct
     5101 tggcgggctt ccgatagttg atcgaggcgc gcaggttcgc caggtccagg ttgatggcgg
     5161 tcgttaattt ttccgcgctc atatcgccgc cacgtccagg ctgataggca agtaatcgcc
     5221 gctggccgcg tcgcgcttgt agaagcggat atagggcttg gtactgccca cgcgcatgct
     5281 gtcgctgatc gcctgcatcg ccagcagcca gttcgggtct gtgatcttca gactgcgcag
     5341 gcccagcacg cggccggtgc tgatcttgcc ctgctggtcc acctggaagg catggttgac
     5401 catcaccttg atattgtcgt tactgccttt gctccagccg gtgatgcagt cgtcgatcag
     5461 cgccttggcc gccataaggc gttcgtcgaa gaccagggtc tccgccatct gccggacaat
     5521 cttgtattcg ccgtcgaagg tgaacagcgt gacgttgccc ttggtgccgc gcggcttgat
     5581 gccgtactcc gccatgctgg tgttgatgaa atcggtgacg gcttgcatag aaacggtttt
     5641 gaaggccatc aggcgtgcgc tttcctgctc ggcttgtcgg cagatgtcgc caaccgtctt
     5701 gtggcgaacc ttgtcgattt ccttgatctt gctgaccggg atcaggctgc cgctggcatc
     5761 cttccagtag ccagggtgcg aggtttgttg ttcactcatt tagaagctcc tgcagtggtt
     5821 gattttttgg aagggctggg gacgcccatt tcgatataaa ggtcgggcgc aaagcggccc
     5881 ttcatggcgg cgacctggtc caccgtggtg ctggcgcgct ccaggaggaa gacgcaggtg
     5941 tcgtcaaact ccttctgtgt ggcggccatg tagtagccgc tggccgggta accgcacacg
     6001 cggtgctggt ccatgcgcag ctccacgatg aagtcgcgca cagcccgctc cagggcgggc
     6061 gggttgctgg cctggttggt gatgcgggca accaggtcac ggccgtggat gccgttctcg
     6121 cggccgacgt gcttttgcaa ctcggccagg acctgggctt tggtgatctg cagctgtgac
     6181 atggttggct cctggttact ggacgatggg cggcgcggcg tggtggtcaa cctcaagcga
     6241 tgccggcacg ctgcagcgcg tcatgttctt gctgaacagc tcgttgaaaa cgatgctgcc
     6301 gccgacattg atcagcgttg tgccggcgtt ttccagcagc ccgctttcgg ttgcgatggt
     6361 cacgaaggcg agcagcaagg cgtcgagctg cacattggcg ggcaggccgg ccagtgcgtc
     6421 gcgcagcttt gactgctgct catgagccag cgccacgagt tgttcggtgg tcaaggtggg
     6481 ctgattcata ggacacctcc aagcgcagtt gctgggggca gatccggcag cgtcttgcat
     6541 tcggacgtga aggcgtagat acgcgtgtgc gctttgtcgc cctggtcgcg gctggccagg
     6601 aagccgccag cctccaaggc atcaacggcc tgctgcacct cgtgcggcgc gaaggtgggc
     6661 cgccagtgat gcgtgagcat ggcggcggtc caccagccgc cgtcgcgcac cagcatcagc
     6721 cagagcgcgc gcgtgtcttg cgtgatggcg ttacccataa atggcccttc cagggatgaa
     6781 gggcatgcgg cggccggcgt ccaggcttgg gcagttggcg aagtccatcg ccccctcacg
     6841 gcacggcact ggtggcggcg gctggtaggt gccgccccgc atgacgtaga cgcggcgcgg
     6901 cggcgtgatg cagccgccag cggccagggc ttgcctggac gttggttcct gctcgccggc
     6961 ctggtagccg gccacgtagt acatgacgcc ttcatgcacc tcgcgcgaca catgcccgcc
     7021 ttgccgcagg ttggtcagcc gggcgcgcag ccagtgcgga ttcgggacga acccgttggg
     7081 gccgttgctg cggtggtagg acggctccct gaacagcaca tgcagctggt caaaggtttg
     7141 acggccgtgc gcctgcaggt gcgcgacgat ggccttgctc tgcggattga gcctggcacc
     7201 cttggggggc atttttttga attgcatggt tcaggctccc ctggtgcaaa gttggcgggc
     7261 tgcgttcatg ccctgcagga aggcgctgtc gagctgcgcg tcgagcgtcc acaagatggc
     7321 gactaccttg actgcaccga gcaccatcac ggccaccacc atgaacagcg ccagccagcg
     7381 caggaagcgc agcagctgct tgcgccttga tggcacggtc ggaatcacga cgcctggata
     7441 aaaggcatgc cggttcatga ctggcctccc tggagcatca ggaagtcctg gcgcgcgtcg
     7501 aacacggcca gcttgcggtc gatgccataa aagttctgca ggcgcctggc gcgccgcttg
     7561 atccatccga atcgggtcat ggtttacctc ctttggggtt gttggggcag gtctggcagg
     7621 cgcgccagag ccgcacacgc gtcgggttgg cgctgtggaa gggctttttc cgctcgtcct
     7681 ggcaaatgcg cgtggtgatc tcgcccaaga tcgggcagac caccgtctgg ttgagcacct
     7741 ggccacggat ccgctctgcc agcgcttcga cgttgccgat gtagcgcccg gccagcgcgt
     7801 tgctgatcgt tccctcgctc acgccgatca gtcgcgctat cgcggcctgg gagtgggtct
     7861 ccttgaggcc cagcagcgcc tgcagcgcgt cggccggcag cggccgggcg accggcgtct
     7921 tattcgaggc catcggcgac ctcctgcacg ggttgcgtcc aggtgaactt gcccaggttg
     7981 cggtcaaaca ccgacttcac gcgcgtgatg gccggcgcgt ggtgcccggt caccttggtc
     8041 agccggtacc ggccggcctt cagccttgtg ggctcctgca gcgtggtgag gtagccggcc
     8101 ttggcgaggg cgttcacata tttctgcgcg gtaatctcgg cgatgatcaa ggtggggtgg
     8161 ctcgcagcac gggcaatgtc cctgaagtcg aaagacttga gaacctgcat gcagcgccac
     8221 atggccagta cgcccaagcc ttgccgcgcc tccttgccgt acaggcccac acgcggcgct
     8281 tcaaaggctg ttttaaccag cttgtagact ggtgcctcga agcggctgcc tgccggtttc
     8341 tccggcttga tgcgctcgac cacctccagg tagccagcgt ctttcagatc gcgcaggtac
     8401 tggctcacgg tgtcgtaggc cacggtcggc ttgcagccgt cctcaaccat catggccgtg
     8461 aactgcttgc gcggcccgaa ggtgcgcacg gactgccaga tccgctcgcg cggcgtcagc
     8521 atgccggtca tcacgatgga aggctttccc gccattacag gcccgccctt ccaggtgcaa
     8581 ggcccgtgta cagctcgaca tcgcagtcgg ccaaggcatc cttggtgccc ttgcggtcgc
     8641 accagcgctt gatgttgtga atgttggtga ccacgcggcg ggtgatgccg tgggtctcgg
     8701 ccagcacgcg ctttttgagt tcgggggcaa tccggatatc gggcgcgtac tgtttgacca
     8761 ggagatcgaa gtcgccgctg gtgcaagccg tggccggttg ccagatcagc acacggttgt
     8821 cgaaccgctc gtggcttttg agcttgttgg gcagtcgctc ttcgccgatc agcaggatgg
     8881 gtgcgtccga tgcgtcgtgg atgtcgcgga tcagctccac cgttttcgat tccacgatgt
     8941 ggtccacctc gtccacgatc agcggccggc cgctgagcga aagcgcctcg acaatcgcat
     9001 ccacgatttc cgggatgctg cccttggcca caatgccaat ggccttgcag atctggatgg
     9061 ccaggctctt cttggtttca aagctgcggc agctgacata gatgccgttg aagccggccg
     9121 ggtgactggc gtaggctgcg ccgacgctct tacccaggcc gctgcggccg tagaaggcgg
     9181 caatgccggg caagccagga gcgcgcgttg tcagcgactc catgagggta tgcaccaggc
     9241 cgatgttgga ggttggcgca attacgccgc cgccagtggc tgttgaaatt ttcctgtttg
     9301 tcatatactt tccttctcgt tagttcttct gaacaccgcc cggtcaggcc gcacgcctgc
     9361 cgggtttctt tttttggatt gcccgctgtc agccggccag cacctggtca gcggtcaatc
     9421 caaaagtctc gaagtagcgc tgcatggatg cccactcggc accctgcgcg tacccctcaa
     9481 accaatttcc ttcgtcggcc gtcagcgcct ggctgagctg gacgcgttcc tgcaacttga
     9541 gccagcgggc gtagcgaatc tggggcgtct cgggcatggc gcgcacttcg gccggcttgg
     9601 ccaggaaagc cgcaaggccg gcctgtgctg cttccacgcg ggcttggtct accaggggag
     9661 cgtcggccgc cacgatggag gcgatggttg cgctggtgtg ttcggcagat gggcgctgga
     9721 ttgagaccac gttgcttgcg ttggccaccg cgtcctcggt ccgtgcccgg ttgatgctgg
     9781 cgacggcggc acgcgtgtcg aaggcctttt ttgcttcgcg caagccgcgt ttttgttcac
     9841 ggatgacttt ggcctgatga gcgcgtcgct cggcagccac ttcggcgcta ttgaggccga
     9901 gcttttcgta gtccagggct tcgcagataa actgcccgtc caggtcgaac acccagcagc
     9961 ggccaaggtt ggtgtcgtcc accttgacct gcactgacgt gccctccatg ccgcccagcg
    10021 atgcgtggtt atagaaacca ccgatatcga tgcctttttt gccaataacc cgcgtaccgc
    10081 tgtatgccgc cggtgcaagc agcacgtcca atgcgcgctc gttgatagtt ttgagctggt
    10141 ggccggcggc catctgcagc ggcgtgcggt cctgcaggta gctgcgcggc ttggcgtcca
    10201 tgcggtcgca gaaggtattg agctgctcct gcacctgctc gggcgtcaga cgcatttcca
    10261 ccgtgaagct tttgtctttc agaatgcgct cagcaaagcc cttgcgtgcc tcgatgcctt
    10321 ttcggtcggc cacgctatgg ccgatgtatc cgtcaagcag ctcaaacaac tggtgcatca
    10381 atgagccgat ggcacgctcg atgtgcggtt tttggtgtgg gctgaatggc tcgcacagcg
    10441 gatgtgcaat ggctaggccc agcagagcca gttccagcac ctgcgcgacg tagtcggccc
    10501 cgttgtctgt cttgatggat tccggcacgc cccagcgctg aatggcaagg cggatcaggc
    10561 tcatgatcgc gttggcccgg ctggtgcgag tcactatgaa caagcgacgg cgcgtgaaaa
    10621 cgtcaatcac gccgacgatg tgatggcggc ggccgtcagc cagcatgatg tctccgaccg
    10681 tgctgtccat ttcccacttc tggttgggac gtgtaatgcc ctggctgtag ctgccggccg
    10741 cagacatgta tttgttgcgc cacgcgtccg ggttgattgc cgcctcgtag acctgcttgt
    10801 tctgttcttt ccaataggcg agcgcgcgac ggaaagcccg gtcactcggt aggtctttaa
    10861 aactgctctg caaggcttca tagatagcct gggctgacag atgtggttgc tgcaccagca
    10921 tggccaggac tgctgtgccg acttcgccgg ccaatgctgc atacataccc ttgcggacat
    10981 gctcacgcgg tgtgatggcg acaaggcctc gctcctggac gccgacgtgc caggcgcgca
    11041 gcgtgctgaa actcagggtc ggaaactggg cgcacagagt gggctcgacg tcaatcttgc
    11101 cggctgccca ttgggtgcaa aaggcttgca gagccggcgt cgccttgccg ccgaacccct
    11161 gccagaagcg ctgaaagagc accaggacgc gggcgatggc gtcctgcctg tctttgcgcc
    11221 atccggtcac cagtgcgccc tgccgtgtgc cgttgacacg gttctcgccc accgattgca
    11281 cggacttcgt gatcgcgtcg ttggctgcgc cctggctttg ctggccagcg aagcgtgccg
    11341 cccattccat gcgggctgct ggtggcaggg cttccaccga atactcaaag ccgccaccgc
    11401 gtcccgccct tggccgggcg gggagctgca tgcgtgtggc tgtctcgcgg gcgcggcgtt
    11461 cgcttgacgg gaaaccgggg agttgggcga gttcggcggc ggtagcaaat gctctcatgc
    11521 gcgggcctct tgggctgtag ctgcgtcggt tgccaaagac gccatctgca cctgagtagg
    11581 ccgccaatgc ttgccaccgg ttggcaaacg cgcagccatg tcggccagcg cattgagctg
    11641 ctgcgccatg acgcgcagtg cgcctgggcg aatttcaagc ccgttgaacg gttggccgtc
    11701 aagcactacc agcgcatggc cgtggcggca acggctcagc gtggcctgga tgggggtggg
    11761 tgcggtcatg ctagcgccct cgccttttag ctgtcaaagc cagcgcctgg agtggtactt
    11821 tcagcgttat gtcgagcgcc ttggtgtttg cactgctgcg cacaggaaga cccatcagtg
    11881 cgcgcagcgc accgcttccg cctccgatac aaccgtattt gccacgcgga gttagcgcgt
    11941 cctgcacaag cgtgtgagga attccaaact cccgctgcca cgcacttacg ctgccgaatt
    12001 tctttttgat tgccgcaacc agctcttgcc tgccaagctt gttgtgcgta ttgaacgacg
    12061 cgtaatctga tggcacagat gccagcgaaa tttgaccatt cagatgaagc ttgtagtgcg
    12121 gcttcacttg cgcgctcccg cgcggcggtc gccagacctg cattcaacac cgtcccaggt
    12181 ggacgcaagc tttcggcggt cctcggttct cgcgaccagt ttgggcttcg ttcgccgcat
    12241 caacgtgggc ttcgcatcag gccagatgga ggccttcggg atgcccagca cctggctaat
    12301 gcgcgtggca accctgtccg aaacaccacg gccatgaatg acctgcgaca cggttgtacg
    12361 ggaaatgccc aactcgtctg caacggccga gggcgtggtg cctttcatgc gaatggctgc
    12421 tttgatttcc tctgggtgca tggctgggtt atccttcgtc taacaagttg gacgctctga
    12481 ttaattgcgt ctgttagatc gaattatggg caattaatta cccatttgca aggtaaattt
    12541 atgtctcatg acgaaatatt ttttgaggcg ggacaacgta ttgcggaatt gcgcggcgat
    12601 ttgacgcaag ccgcctttgc tgagcgactt ggtgttgacc gaaaaacggt tgtgggctgg
    12661 gaggctggaa agcgtctccc agacggttca tcgttgctgg cgctggcacg ccagttcggc
    12721 gctgatgtga actacatcct cactggacgt gcggaagtgg cggctggagc gctgcagcct
    12781 agagaaaggg cactcctaga taactacaag cattccggta aggaggggca gcgcgccatt
    12841 gagcgcgtcg cgatgctgga agcgaaacag ccagagataa gtgttaaaaa agcggaaagg
    12901 aaggcagcat gaaaaacaat gagctacaca ggcgcacacg caggcaagat gcagccagga
    12961 aagggaagtc atgagcgcta ctgcatattg ggtcttggcc acttacgcgg ctatcgttgc
    13021 ttggtgcgtc tggtccggcg tgcgatcacg ttgggcatcg cgccaagtcc gcgtgcttgc
    13081 ttggacgctg ctgccggctg gtgtggttgc tgttttttct attggtctat ggttgcaatc
    13141 gccggtgtcc gaagaacaga ccagcgcgac ggcgcaaaaa cagaggatgg tcaacatggc
    13201 ggcgcaactt aaggccgcag accgcaaggg cgacgcgggc gcggccgcag cgctgcttga
    13261 cagttccaca gctgtgtttc gcgcactcaa tccgacaggt gcgcctcaag cagacaaaaa
    13321 ggattgctta cttgctatcg gtcatctttc tgatggaatc actgacgtca tgcgtggcgg
    13381 ctcctggatg tcgcagtcac gtttcgaggc ggctttggca gactgcaagc attagtctag
    13441 agtgaccgtc gtcactctga caaatcaagc cccggcccgg cattctgccg ggcatgccca
    13501 accaaaatca aactccgctc gcccgccaag tcgggcaacc gctgccacgg tcgattgacc
    13561 tggttgtcat ccactgcgcc gccaccccca gcggcaaacc actgcagcag ggcaagcccg
    13621 gctcagtggg ttacatgaac gcgccccagg tcattgacgc ctggcacgcc ggacgcggct
    13681 ttcaccgcaa ccctgcggcc gtgcgcgcct tcagctccaa gctgccgtcc atcggttacc
    13741 actacgtcat cgacctgacc ggcgaggtct ggtctggccg ggccttgacc gaggttggtg
    13801 cgcatgcggt cgagttcaat tcgcattcgg tgggcatctg cctggtaggc ggcgctgagc
    13861 gggaagcgca gtacacggcc gcgcagtgga agagcctgca gcaggttgtg gcgatgctac
    13921 tgaccgacta cggcatccct tgcgccgcgc ccaagcgcgt gccggacaaa acggccaagc
    13981 tgggctatcg catgttcggt ggcgtgtgtg gccaccgcga cctgtcgccc gatggcgatg
    14041 gcgacggcat ggtcgagccc ttcgagtgga cgaaaacctg ccccggcttt gacgtgcgtg
    14101 cgtggctggc caacggaatg cagccgctgg ccaagcacgt ctaccaggag caggcagcat
    14161 gaggcacgcc gcactcctgg ccgctgccag tctggcgggc gcattcggtg ccgtggggtc
    14221 actggccacc gtcaacctgg gcgaagttcc aggtctgtca cagaccacac agcgcgccat
    14281 caacaagagc gcacagaacc cgatcaagga agccagcaac aagatgcgcg caatgctcgg
    14341 tggtggggtc ggcacctcgg gcgggcgcca gcgccgagca gcctatggct ggaccaaccg
    14401 gcacgctgtg cgcgtggcgc gcaagcgccg caaccaggcc cggcacaagg cggccggccg
    14461 tggagggcgc cgatgaaatc ccggaactca atttttatgg gcggcccccc ggcgcgcatg
    14521 cgcgaaggtg atagcccgcg ccgcgtgtcg agtgggcttg cccaccactg cggcgaggtg
    14581 cgtgaatgag cccgcaagca ttcaaggctt tcaagcacat ggcggcggcc caggcagcgg
    14641 ttcccgccac gcccggcgag cgcgcttacc gggattactt cctgctgcgc aacggcgtca
    14701 actccgaggg cgggccattg cccgcctggg gcgaactgag catcgagatc cgcagctggt
    14761 gggagcatac cggcgtgcag ccgccaggcg cgccgcgctg gtgggagaac aaggtgttct
    14821 ggttcaacgc catcgtgctg gccgctgctg ctgcagaaga gcgcatgggc ctgttgaaag
    14881 acgttctgcc cggcagcgtg tttgtctggg tggcctttgg cctgccggtg ctcaacaagg
    14941 ccatccgcgc cttccagctt ttcagggcca tctcatgaac tgggtgcgca tcgccatcat
    15001 cgccgccgtg ctggcagcac ttgccgctgc cggcctggcc gtgcgctcgc acctgatcgg
    15061 tgtgggtgaa gcccgtaccc aggcgcagtg ggacaagcaa aaagccgttg accaggccga
    15121 aaccctgcgc ctggagcgtg agcgcagcgc cgaccaactg atcaagtttc gtaacgcaga
    15181 aaggatttcc gatgaacagg caaaacgcga ggcgcttcgt gagcgccgca tccgtgctgg
    15241 cgatgctgtt gctgaccagc tgcgcagcgc cattgaaacc ctcaaccggc gtgacgtgtc
    15301 cgccgccggc agcgatcccg gatctgtcgc cctcgctgaa ggtgccgcca ccgcccgaga
    15361 gctttttgga agctgcaacc aagctctcct cgggctggct gcagaagctg accgactcag
    15421 cgaccaagta gccggcctgc aagacgacgc gatgtttgta tgtcgcgcac ccaaacaaac
    15481 aaacatggag agcgcgcctt gaaagttgaa tttgatctgt ggcagttgat cctgctcgcg
    15541 tccatgatca tgggcgcttt ctggggtatg gccaagatga tgctggtgca gcaggtgaag
    15601 tttctggacg cgaagttcaa gaccaccgat gacaggttcg aagccatcga tgacaagttt
    15661 gaagccatga acaaacgcct gaccgtccag gacgaaagcg accgccgcat cgagcgcgat
    15721 atcgcggcct tgcgcgtgga actgcccacc gattacgtgc gcagagaaga cttcacgcgc
    15781 gtgatcgcca gcttcgagat caaagtagat aacctgcgcc tgacgattga gcgcgccatg
    15841 ttcggagaca agcgtgttta accagaccat cactgacaag gttcgccgcg aggcagtgcg
    15901 ctggcacctg atgcagatcg ccaacgtctc gcgcccccaa ggcatgtaca ccggcgcgat
    15961 gctgcccatc ctgcaatccg tctacccgga cgcctccgag ctggaggtgc gccggcagct
    16021 ggactacctg caagagcggg acgtggtgaa gatcgacata gatccgatgg gccggtggtt
    16081 cgtcacgctg acgcgctggg gcgtggacat cgtggaatac acggtgccgt gtgagcctgg
    16141 tatctcgcgc ccacagatcc acggacaagg gtgagcggca atggtgaaac gcagcaaggt
    16201 agacacgcta ccgcaggagc tgaagaattg gctggacgcc gagttggtga cgcgcggctt
    16261 tgctgactat gtccagcttg ccgccgacct gcagaagcgg ggcgcggctc tgtccaagag
    16321 ttccctgcac cgctacggca gcaagttcga agagcgcatg gcccagctca aggtcagcac
    16381 cgaacaggct agagccgtcg tggcggccag cccggacgat gagggcgcaa tgaatgaggc
    16441 gctgatccgt ctcacccagg acaagctgtt cggcgtgctg gtggacctgg acgttgatcc
    16501 cgagtcggtg aatatcacca agatcaccaa aagcattgcc gacctggcgc gcagctcgat
    16561 cacgcagaag aagtggcaga tcgaggtccg taccaaagcg gccgagcgtc tgaaggctgt
    16621 cgaacaggag gccaaggcgc tgaccggcgc atcgcgcgat gttgcgatgg agatgctcaa
    16681 caaggttcgc gcggtctatg aaggggccat gtgagccagg ctgtagacaa cgctgtccgc
    16741 gcactgctct attcctacca ggtcaaatgg atcagggaca agagcaggtt caagctcggg
    16801 cgcttcgcac ggcagacggg caagactttc acgaccaccc tggagatcgt tgacgacgtc
    16861 catgaagccg agatactgaa gcggcgggca ccctgggtca tcttgagccg tggtgagcgc
    16921 caggcacagg aggcgatgga ggaaggcatc atgcggcaca acgccgccta tggtgccgcg
    16981 ctgaaggtcg cccgcgagga cactgacttc tatgatgaag agagcggcat caagcggcgt
    17041 gcgctgcagc tgatctaccc tggcggcaac aagatcaccg cgctgcctgc caacccggac
    17101 actgcacgcg gcttcagcgc caatgtgttc ctggacgagt tcgccttcca caaggacagc
    17161 cgcaagatct ggacagcgct tttccccgtg atctctgccg gctggaagct ccgtgtcacc
    17221 agcacgccaa acggcaaggg caacaagttt tacgacctcg ccacggctac cgctggcggt
    17281 tggtcgcaac acgtcgtgga tatctatcag gccatcgcgg ccggcctgcc ccgcgatgcc
    17341 gatgagctga aagcggcgct gatggacgag gacgcctgga atcaggagtt cttgttgcaa
    17401 tggctcgacg aggcgtcagc ctggctgagc tttgacctga tcaacagcgt cgagcatgac
    17461 catgccggcg tccctgagtt ttatacaggt ggcccgtgct acgtgggcgt cgatatcgcc
    17521 agacgctcag acaatttcgt gatctgggtg gacgagctgg tgggcgacgt gctttggaat
    17581 cgcgagacca tcgtcaagaa gcgcatcagc tttagcgaga tggacgcgct gcaggacgac
    17641 gtcgagctgc gctacaacgt cgtgcgctat tgcatggacc agaccggcat gggtgagaag
    17701 cctgtcgaag atgcgcagaa gcgctacggc aaaaatcgtg tcgagggcgt gctgttcacc
    17761 gggccgaaca aactgcagct ggcgacgttg atcaagcaga gctttgagga ccggaagagc
    17821 cgcatccccc aagcggacga tccgctgcgc ctggacctgc acaaggtgcg gaagatttcc
    17881 agcccaaccg gcgcgccacg ctttgacgcc gacagtgacg ccaacggcca tgctgaccgc
    17941 ttttgggcaa aggcgctggc ctgcctggca gcgcacggat cgctcggccc cttgcatacg
    18001 gcgagccggg ccaggacgtc caccgcatcg gtcgccagcc agcttgcggg gtaccttcca
    18061 tgaccccaat ttgtgagcgc tcgctgactt tccgggcgag gcctgccccg gcgcgttcgg
    18121 ccctgcatgg ccgcctaccc cacgccgatg gcctccaagg gcctttcgtc ccccttttaa
    18181 atggctgcaa accgtatttg cgaccccaag acacctctag gaaaacgcca tgacccgtgg
    18241 actgtgggtt tcccccaccg aatttgtatc ttttgagcaa caaagcaaaa cggcggctgg
    18301 cggcttgacc ggccagatcg cgacgcgcct ggcagccggc gacatcagca gttttatggg
    18361 cctgctgccc aacccggacc cggttttgaa ggcaatgggc aaggacatca aggtctaccg
    18421 caacctcctg gctgatccgc tggtgatggg cgcacgcggt cgccgcgcgg ccgctgtgct
    18481 gtcgatggag cgtggctttg atgccacctt gtccaggcgc acgcccgttc gcgtgatgaa
    18541 agcggtcgag gccgtctttg ccggtctgga cctgcagcgc atcgtccgcg acctgatcga
    18601 cggcgcattc tttggctacc gggtcggcga gttgatgtgg gagccacgtc acggcctgat
    18661 ggcgttgcgt gacgtgatcg caaagccctg cgagtggttt ggctttgacc ccgaagacgc
    18721 acggctgatg ttccgtccct ggaattcgtt ccagggcgtc gatgtgcccg accgcaagtt
    18781 cgtggtggtc ggcaagatgc gcaactggga aaacccatac ggcgagcccg acctcgctgc
    18841 ggtgttttgg gcggtgacgt tcaagcgcag cggcatgaag ttctgggtca ccttcaccga
    18901 gaagtacggc atgccctggg cagtgggcaa gctgccacgc caggccgggc agactgaggt
    18961 cgatcatctg gccgacaagt tggaggcgat ggtgcgtgac gcggttgccg tggtgcctga
    19021 cgattcgagc gtggagctgc tgacgactgg cggcactacc agcgcggaca tgtacgagaa
    19081 gctcctgatg ttctgccgct cggagatctc cgtcgcgctg ctcggtaaca accagagcgt
    19141 ggagatgcag agcaacaagg caagcgccac ggccgccaag ggcgtcgagg catcgctgcg
    19201 ggatgccgac gctcagatgg ttgctgcggg cctcaatgcg attgctcgtg acgtcaccga
    19261 ggtgaacttc ccaggcacac ccccgccggt gtatggcttt tgggaacagg aagaggtgga
    19321 cgaggtacag gccggccgcg accagaagct caagaacgcc ggtgtgcgat tcaccaatca
    19381 ctactggaag cgaacataca acctcgaaga cggcgacctg atgcccgagg ctgccggcac
    19441 tgcggatccg tccaacccgg acgacaccgt caacttcgcc gatcccaaca ccgacgatgt
    19501 gccggccgac cagctggccc tggacgccgc catcgagcag ctgccggcgg atgctatcca
    19561 ggcggccatg aaaaagctcc tggcgccggc tttaaaggcc attgaaatgg cctctacacc
    19621 cgacgaggtg cgcgaggccc tggtggacgc ctggccggac atggatgcca gcgacctgga
    19681 ggaactgatg acgcgtgcct actttgtcgc tgacctggtc ggccgtgacg gcgcggcgca
    19741 ggaggcggcg tgagcattga cgcgcacgcc atattggcgc aggtaaaacg caaccaggcg
    19801 ttgcttgata gctgtcaaca gcatgccttt accccaacac agatgcagca tggcttcgtg
    19861 cggcgctata tgtgttcgca ttgccagggc gaggttgatt cgcatggttt ccattggtat
    19921 agcaaaggct tggcccacgc cgccgcagcg cagacatgac gatcagctcg gccgatatcg
    19981 gtcgctccat caccctgccg cccgaggacg ccatccgctt cctgcaggcg aaaggcgcgc
    20041 aggtgacagg caactggacg gaatggctcg acggtcagca tgcccgtggc tttaccgtgg
    20101 ccaacgtggc caagctggac gtgctgcagg acattcaaaa ttcgttgatc aagtcacagc
    20161 aggacggcca aacgctgcag cagtggaagg acggactgat tccggagctg cagcgcaaag
    20221 gctggtggcg gcgcgagggc accacggcgc agctgcaggc ggctgggcgt gtcgataccg
    20281 atggcgtgat cgccaagggc ctgacgccgt accgcttgaa gacaatttat cagacgaaca
    20341 tgcagagcgc ctacatggcc ggtcgctatg cgcaaatgat cgagcaggcc gaagagcggc
    20401 catattggca atacgtggcc atcctggacg gcaagacgcg gccggcgcac cgtgcgctca
    20461 acggcaaggt gttccgctat gacgacgccg gctggggcgc gttctatcca cccaacggtt
    20521 ttaactgccg gtgccgcgtg cgcaacttca gcaaatcgga catcgaccgc cgcaaaatcc
    20581 cgctgagcag caccgagggc aagctacgcc aggtgcaggt gccgctgaag agtggcggga
    20641 ccgctgacgt gacgcgatat gtggaccgct cgctgcctgg tggcaagttt cagccggacg
    20701 ccggcttcag caacaaccca ggcatcaacg cctaccagcc acgcctggag ccgctggaca
    20761 cacagctgtc gcgccggtat gtggacacgg tgatgcaggg accttctttc cagcgcttcg
    20821 tagctgcaca gtccgaaggc gtgttcccgg tggccgtgtt acggccggcc gaccaggcgc
    20881 ggctgcaggc cgacacctct gttgcatacc tgagtagcca gacggtgacc aagcagctgc
    20941 agaaccatcc ggagatcggt ctggacgact atcgccggat cccggacatc gttgaccagg
    21001 gcgacgcgca cctgcagggc gccaatcgcc ttgtgttctt gtttgaggac ggtgtggtgt
    21061 ggcggctggc cttgaaagcc acgaagcagg ggcacgagct gtatgtgctg agcctgttca
    21121 aaaccaccag ggagaaggcg cagcgcgaga tcagggagcg gctgcagcgc aattaaacgg
    21181 ggccagtagg ggtatccagc cccgctagag ctcgcacctg gcggtgctgc ctcggaagga
    21241 ttccgacact ggcccgccaa tgataggaca aaaccgtgat tcagtctcgc atcgaataca
    21301 aacccgtttt gaacgccctg cgtcaggctg ccggccagat gggcgacaag cggccgctca
    21361 tgcggtcagt cgctggcgtg atgttccgcg ccgtcgagga caacttcgag caggaggggc
    21421 ggccaaagtg gaaagacctg catccaggca caaagctcag ccggtacaag cagggcacct
    21481 ggccaggcaa gatcctgcag cgctcggccg gcgggctggc ggcgtccatc gtccagctct
    21541 tcgacagcaa ccgcgccgtg gtgggctcga acaaggtcta cgccgcaatc cagaacttcg
    21601 gcggtaaaac caagccgcat gtgatcaagg ccaaaaacgg ccgcgcgttg tcgttcggtg
    21661 gcatcgtggt aaggtcagtc aagcatccgg gcagcaacat accggcgcgt gaattcctgc
    21721 gattgacgcc cggtgatctg cgtgaaatca tcgcggttgg ccggctgcat tacgccaaag
    21781 cgctggcccg caacggactg caaagccgct gagcccttcc gctcaaagtc gcgcatttaa
    21841 gtgactgccg tcactctgcg catgagccgc ctcaagcccg acagttgcgg gcatggcatc
    21901 acttcacatc tcgaaacccg gccgtgcagt cagcatggaa gggatcgtcg tcgatttcac
    21961 gtcgtcgatt ctggaagagg tggcttccac ctacgacccc aagctgcacg aagcgcccat
    22021 cgtgatcgga cacccaaaac tggcagatcc tgcctatggg tgggtcggta gcctgagctt
    22081 tggcgagggt ggcttggtcg ccgacgagcg ccaggtggat cccgacttca acgaactgcg
    22141 ccgcaagggg cactacaagc atccgtcggc atcgttctac atgccggact cccccaacaa
    22201 cccgacgcct ggcaagtggt atttgcgcca tgtgggcttt ctcggtgcta cggctccggc
    22261 gctcaagggc ttgaacgccc aggccgtgag ctttgccgaa tccgagcagg gcgtcgtcac
    22321 gtttggcgag ctgcctggtt acgccggcag ctacgtggcc agcatgcttc gcaagctgcg
    22381 cgaatggttc atcgacaagg aaggcctgga gacggccgac cgcatcttgc ccgactggga
    22441 agtcgagggc ctgcgcgaga tcagccagcg cgcgtctgat tccccaacgc aggccacccc
    22501 gcaaatcacc agctttgcag acccttccaa cccgcttttc aaccacaagg aaccgccgtc
    22561 aatggacaaa acccctgaac aactgaagaa agagctgacc gaggcccagg gcacgatcag
    22621 cagcctgcag gaggccgacc gcaaacgcgc ggccgacggc cgccacgcgg cgcacgtcag
    22681 cttcgccgac accctgatcg ccgaggcccg ctggccgtcc ggtgccaagg atgtcctggt
    22741 cgccacgctt gaccacctgc agacgccatc gggcgaggca ggcgtcgtca gctttggcga
    22801 aggtgacaag gccagcccgc tggccgtcgc actccaggag caactcaagg ccctgtcgcc
    22861 gatggtggag ctgggcgaag tggccaccaa ggccaagggc gctggcgcag ctacaggcgg
    22921 cgtcgtgagc ttcgcagagc agcccggcta tgccggcata caggtcgatc ccgtgcgggc
    22981 tgacctggac aagcgcatca aggcgcacat ggccgagcac aaggtggact acgccaccgc
    23041 cgccagccag ttgaccaatt aacccaaacc gcaaggagta ttttctaaat gggacgtctt
    23101 tccaacatcc gcatggtcga tccggtattg accaacctcg cactcggcta cagcaatgcc
    23161 gaatacgtcg gccagcacct catgcccttt gtgccggttg acaaggaggc caacaagctg
    23221 ccgaagttcg gcaaggaagc cttcaagatc tacagcaccg agcgcgccct gcgtgcgaag
    23281 agcaaccgga tcaatcccga aggcatcacg ctggtggacc tgaacaccga cgagcacgac
    23341 ctggaatacc caatcgacta ccgggaagat gccgaagccg cgtttcccct gcaggcgcac
    23401 gctaccaatg tcagcaccgg cggcatcgcc ctgcgccgcg agaagatgat cgctgacctg
    23461 gcacagaacc cggccaacta cgcggccagc aacaagattg cgttgtcggg caccagccag
    23521 ttcacccatg cttcctcgga cccggtgaac gtgattgagg acggcaagga ggcggtgcgc
    23581 gccaagatcg gcaagcgtcc caacaccatg atcatcggcg cgtcggctct caagccgctg
    23641 aaattccacc ctgcgctgct cgacaagatc aagtacacgc agatgggcgc gatccgcctg
    23701 gcgcagctgc gcgagctgtt cgagatcgag aacatctacg tcggcgaggc ggtctattcg
    23761 tccgacgcgg atgtgttcgg cgacatctgg agcgacagca tcgtgctggc ctacgtgccc
    23821 acccatcaga gcagcgtcga gcgcaacctg atggagccga gctacggcta cacgccgcgc
    23881 cgcaagggca atccgctggt ggacgtgcgc accgaagacg gaaagatcga agtggttcgc
    23941 gccacggaca acttccgccc gtacatggtg gcggccgaag ccggctacct gatcaccgac
    24001 accaacctct gataaggatc gcccgccatg aaatacaccg ccaacactcc catcctgcac
    24061 gacggcgaac gctacgaaga aggcgacccc atcgagctga ccgacaagga cgccaagcgc
    24121 ctgggcgcga aggtcacgcc ggccaagccc gccaaggaag aaaagaaacc cacctgacca
    24181 ggactgacgc atgaaaactg aacaaccgat ccttaccacc agcattcccg cgacggccgc
    24241 gctgacggca cgccgctttg tcaccacggt cggcgctgtg ccggcggccg gcgcgaaggt
    24301 gctgggcgtc gcaaacgccg gctacgacgc cggcgaacag gccggcgtcg gcgtactggg
    24361 catcttcctg gtggaagcag gcgcagctgt agccgccgat gccaacgtcg agaccgacgt
    24421 ttccggccgg gcgatcacgc ttgcggccgg cgtggattgc ggccgcgcaa tggacgctgc
    24481 atctgcggct ggccagttca tccgcgtcgt tcgcggcatc tgaccaggtt gatatgccct
    24541 acgtcacccc cgaggaattg attgaccggc tcggcacgcg cgaggcaacg gccatcagtg
    24601 accgccagaa aaccggtgcg ccggacctgg tggtgctggc cgacgccctg gcgctggccg
    24661 aggacgaggt caattcctac gtagggcgac gctacgcctt gccgctgatt accagcggtg
    24721 gtcagccggc agcaataccg cctaccgtca agcgcctggt gattgatatc gcccggtacc
    24781 gcaacaccgg caccgaggtg atggagaccg agcccatccg caaccgcttc aaggatgcgg
    24841 tgaagctcct ggagcagatc tcgcgcggcg agatcaacct gggcgacctg gcgctggtcg
    24901 gcgttggtgg ccctacgcct gtgggcggca gcgttgctgt tcgcaccggc gcgaagacct
    24961 accccgacct aagcggtgtg ctgtgagccc ggttactcag atcgaactgg ccatcgtgga
    25021 gcaactgcgg gccgtgcagc gcccctacac gggcctctgc gtggaaagct atgcggccca
    25081 actggacgac gaactgttcg cctgggtgcg cactgtgccg gccgtgtggg tgacctttgg
    25141 ccaggcccag gacgtcaaac gcaccggggc gcgcagcttc ctggtgagcg gcacgtttga
    25201 ggtactaagc gcccagcgcg ccctggacga aaacgaccgg cggctcaagc ctggcacagc
    25261 cggcgacagc gtcggcgtct accagatcgt tgaagacaac aagctggccc tggtcaatca
    25321 gaccttggga ctggagatcc agccgctcac gcctggagcg attcgcccgg tgatgaaaag
    25381 catggtgaac cgcgacgcca tcgccgtgta ctcccaggag ttccggacgc aatggatgga
    25441 aatctatccg gatcccgaga tgacgcctgg cggcacctta gtcaccgtgg gcctgaacta
    25501 cctgctcaag ccaggtgatg aaaccgtcga ttcgtccgac accctgacga caagaacaac
    25561 ctgaaggaac tgcaaatgct agtgaaagct gcccctggac tgaacgtgcc gcgcgaggac
    25621 aacccgcgca agtacatcac cgacgccgag cctgtcgaga tcgacatgac cggctattac
    25681 atccggcgca tgtcggccgg cgagctggtg gaggttgccg ccgagccggt ggtccctaca
    25741 cccaccgagg tgagttcgcg caagaaatag cgcccgccta caaccaggca gacacctcgt
    25801 ttcagcaaac gttttacaga ccacccggat atcacaccag ccactggctt cactgaggac
    25861 accccgacat ggcaagcccc aacatcagct ttgacagcat accgagcagc atccgcaagc
    25921 ccggcaagta cttcgagttc aacgcccggc tcgcagtgcg cacgctgccc agcaacctgc
    25981 agcgcgtcct gatcgccgcg cagatcacga cgaccaccat cggcctggcc aacaacatcc
    26041 tgcagatctt tgacggcgaa acggccgccg ggctgttcgg acgcggcagc caggcgcatc
    26101 gcatggtcaa ggccacgatc aaggcgaatc catacacgca gctgtttgtg ctgccaatgg
    26161 ccgacagcgg caccggggtg gccgctacgg caactgtgac ctttaccggg ccagctgccg
    26221 gtgcaggaag cgtgacggtg aatgttgagg gcgtggagat cgtgacgcct gtcgccgcag
    26281 ccgacacggc gaccatcatt gccgcggcgg cggctgcagg tgtcaacgcc aagatcgacc
    26341 tgggcgtcac ggccgcggct gccctgggcg tggtgacttt cacctcccgg aacaaaggca
    26401 ccgtggccaa tgcggtgaag ctgtcaactc gtgcagatgc cacgggcgtt acggccgtgg
    26461 ctaccgcctt cactggcggc ctgaacgatc ctgacctggc agtgcccctg gcggcggccc
    26521 agcagggcgg ccacgagatc ctggtagtgc cctacaacgc caccgccccg ctgacgttgc
    26581 tgcgcaccca cctgcagttc gtgagcggcc cgatggagca gcgacgcgca attggcgtat
    26641 gggccagcac cggcaccttg tcggctgcca cgaccttggc cgccacgctc aactccgagc
    26701 gcatgacctg cggcctggtg cccgattccg gcacgcctgg cgaggaagtg gctgcagcgt
    26761 atgcagctgt gatctccttc gaggaagatc ccgcgcggcc gctgaacacc ctggagctgg
    26821 taggcgtgca gccaccgccg attgccagcc ggctcacgcg caccgagcag gaaagctgcc
    26881 tcaacaacgg cgtcacgcca atggaggtcg gcccgggcga gcgcgtgcag atcgtgcgcg
    26941 caatcaccac gtacacggtc aacccgggcg gtgtggctga tatcagctgg ctcgacctga
    27001 ccacgattcg cacgatggac tacatcgcca aggcatgccg ccagcgcatc gagctgcgct
    27061 tcccccgcga caagctgagc tcccgcaccc cggcacgtgt gcgcagcgaa ctgctggacg
    27121 tgctgctcaa ggccgaggaa ctggagatcg tggaagaagt cacggccaac ctggccggct
    27181 tgatcgtgga gcgcgattcc caggatccga accgcctcaa tgcccggatc ccgaccgatg
    27241 tggtcaatgg tctgcacgta ttcgccggcc gccttgacct gctgctgtga tttaaacccc
    27301 ctttgaagaa agactgaaac atggcactga ctgaatacgt gggcttggcc acgctggacg
    27361 tggacagccg cgaagtggag atcgccagct acagcgtcac cgacatgacc ggccgcaagc
    27421 cggtcaagac gatgaaccgc acggggcgca tcaagggttt tacccgtggc ctcgggcagt
    27481 tcgagatcaa ggccacggcc gtgatcccga aaggcggcac gccaatcaac tgggcagaaa
    27541 ttgagggtgc aaagctcacc atcgaaccgc tgggtggcgg caagcgcatc agctacctgg
    27601 actgcttcac tatccaggta ggtgagcagt acgacaccga gaacgaggcc aagatcgacg
    27661 tgagcctcat tgccatccgc aaggttgagg aatgagcttg accgagaaag gcaccctgct
    27721 taccggtgcc gtggtgggcg gcgtggcgca caagacgttt gagctgcggc tgcctaccgt
    27781 gcgcgacaac atcgatgctg tggacgaagt aggcgggcac aacggcgtcg cactgggcgc
    27841 agcgatcctc gcgcgccagc tggtcagcct gggcacgctc aagcccgaac agatcgactt
    27901 tgacctggtg gctgggctcc atcccggcga ctacaacctg ctcgacgcgg cggccaagga
    27961 gctggaaaaa aagcggttgg ccgtcgcagt gagtccggct ggatccgcat ccgcctcgca
    28021 ctctgccgcg ccggcctgaa ttgggacgaa accgcccgga ttgacctggt cgatgtgcgg
    28081 tcgatcctcc aggctgccgg cggaaagccc actgcggcca cacccaccac gtttgtcagt
    28141 ttgcgcaaga aaaagccccg gcagaagaaa cccagtaaat gacacaagat ctgcgcatcg
    28201 ccctgaccac cagcctgaat gatcagctgg ttgggccatt gcgccgagcg ctggacgaga
    28261 ttgagaagaa tctcaagaag gtcgagcagg agttgacggg cgtagcgcag aagagcgacc
    28321 aggcagggcg ggcgctggcg ggcctgcagg gccctgaccg cgctgccaag caggtggctg
    28381 atctggcgcg caacacgcag aacgccgtca gcatggccga ccgccttaaa gccgcctggg
    28441 ccggtgccgg caacatgatg cgcggcgtca cgaccggcat tgcgtcctac caggctgcga
    28501 agtacgtggt ggccgcaccg atgcagcaag cccgtagcta cgacaggcag ctggccgatg
    28561 cggcaaatac cgctttttcc agccgcgacg ttgccggccg gcgcgccggc atggcggatc
    28621 tggatgcgac ggtgacgaac tcgcttcgag ctggtggcgg tagccgcgac caggcgctgg
    28681 ccgccctgaa tgaaatgctg gccagcggtt cggtgtcggc cgaccaggct aagggcctgc
    28741 taccgaccgt ggcaaagtac gccactgccg gcaacgccag catgaccgag atctccacca
    28801 tcgtggttcg ggcgcttcaa agcggcttca aagaggcaga catccccaag atgctggaca
    28861 tggcgctcga cgctggccag cgcggcggct tcgagctgaa ggacatggcc aagtggctgc
    28921 ccaagctgct ggcggccggg agcatgtccg gcctcggcgg catggacggc tacgcccgta
    28981 ttctcgcgtc ggcgcaaggc actgtaacca ccactggaac caaggatcag gccggcaatg
    29041 cgctattgaa cttgctgctc aagatcaaca gcagcgacac cgccaacgat gcgaaaaaga
    29101 tgggcatcga cctggccggc agcttgtcgg atgcgcgcgg caaaggcgtc aactccctgg
    29161 acgcgtttgt gaacctggtg gaccagatag ccgggcgtga tagccgcatg gttgacttgc
    29221 gaaagaaggc ggtcggggcc ggcaccaacg aagagcgcaa agctacgtac aacgcccagg
    29281 ccgacatttt gcagggctcc gccattggca aaatcgtgca ggacagagaa gcgctattgg
    29341 cggtagttgc cgagctgaac aagcgtgact acatccaggg aatctccaag ggtgtgttgg
    29401 gcggcaatgg ccagctcggc aacaccaact tcggcctcat atccagcacg gccgacttca
    29461 aggtagggca acaggaaaac gaaaagctgt tcgcgcagac gcgggcgctg gacagcgcca
    29521 acggcgcgct gggcaagctg gccgaagtgg ccactgcctt ctatcgcgag tatcccgcct
    29581 ttaccagcgt ggtggaagcc gccaagctgt ccatcatggc cttggcggcg gcttctgcgg
    29641 ccgccagcgt ggcccagttg gtcgcgggcg gcggcagcat gctgggcctg ctcaagggcg
    29701 ctggcgccgg cattgccggg gtgggtggcg cagcgatgac cgccttgggc gctgccggca
    29761 ccggatccgc tgccgctctc gctgcaggcg cggccggcgt cggcattgcg ggcgcggccg
    29821 gctacggcgc gggcactctg atcagcaagg ggatcgaagg caccagcgtg catgacgaga
    29881 tcggcaagct ggccgctgca gtgatggcca cgttgggcag caaggatgcc caggaggcgc
    29941 ttgatcgcat ggccgagttt gatggcaagc gtagcgccga ggctgctgcc acgatgcagc
    30001 gacttgaggc cgtggcgcag cggccgatcc gcgtggtgct ggacggccgg gaaatctctg
    30061 cgcaggtgaa ctcgtacaac gaactcttca tcaggaggaa ctgatggcct ggaaagacac
    30121 attgctcgac gcgaagtttc gcggcgtcac ctttccagtg atgaatgtcg gccgcagcgg
    30181 ccgccgcgcc atcgtagcca atgagttcgc ctaccgggca ggtgcggaac ttgaggacac
    30241 gggcctgcgc gcgcgcacgg tgaagctcaa ggcggtcatc tacggcgaca gctacgagga
    30301 ccaactggac gagctggtca aggccctgga agcgcccggt gccggcgagt tgatccatcc
    30361 catccacggc agcatgaccg tgatggccga cgcctgggac gatgaacacg acgccgatct
    30421 ggtggacggc gtgatcgtga atatgagttt tatcgagcac acggtgcgcg atatcgtttt
    30481 cagtgccgac agcgccagcg ccaagacgga cgcgatcatc gccaaggcgg atgcggcgcg
    30541 ggctgcggcc gacgatgcca tcgtgcgccg ggtggacagc gccttcggca gccctttccc
    30601 ccgcctgacc gttctaaaag acctttttga ccaggctaaa acccagctca cgaggctact
    30661 ggacggcacc gccctgcgcg tgatcctgag cgaccttgac ccgctgctgt acccgcgcgc
    30721 ctatgtggca gatctacggg cagtgctgga ccgtgccctg cagggttttc ccttcggtgg
    30781 ccggaacatg tcctttgacg caagcagcgg tgcggccgtc gccttgggct ctggcctggc
    30841 tgacttcaac accgcgcaac tacagctgca gcccagcgcc atagccgtgg caccagcact
    30901 gcccgtgcct gacgcggtca tgctcgaaga cgctgcggct gtgcaggcgc atgcccaggc
    30961 ccacagcgcg acagccctgg ccgagtgcgc cgcgattgtg ttggcagggg agcttgagca
    31021 ccccatcctg gagcgcgccg aggtagaggc attggccggg cagacccgcc aggcgctgca
    31081 ggaggccatg aacgcggcac gggcggctct tgatggcgaa ggccggggcc tggtggccac
    31141 ggcgctgcgg gacgtggcct accaggtgca ggaagcagcg ttggccgtga tcaaccagcg
    31201 cccgcccctg gtgcgcaagg aaagcccgat aagcggcccg gcgcgcctgg ttgcgcatgc
    31261 gatctatggc gacccctccc gcgcggcaga aatcatcaag ctgaaccgcc ttggccggcg
    31321 tgtatttgtg gagcgcggtg acgcgctgag tgtttatgcg gcctgatgtg gtggaagtga
    31381 aaataggcgg ccagatccac acggactggc agagctatga aatcgacagc gacctgatga
    31441 cgccggccga cgcctggcgt gtcacgctgg cgcaggctca gatcgtgctg ccccccgagg
    31501 tggtcgaggg cgcggctgtc gaagtgcggc tcaaccgtga catggtgctg agcggccgcc
    31561 tggacgagcg caacctgcgc gtgggcaaag ggcagcatga tctcacgctt ggcggccgcg
    31621 acggcgctgg gattctgctg gactgttccg ccccggtgct ggccggccgc ggtttaacac
    31681 tggaggacgt ggtggccaag ctggtgcggc cgctgggcgt cacgcggatc cgcatcgagg
    31741 ccgacaccaa gctgctgcgc gagaagatca acaccgagcc aggcgacacc gcctgggact
    31801 cgttgcgccg ggctgccgag gcaaatggcc tctggccgtg gtttgagccg gacggcaccc
    31861 ttgtggtggg cggcccccgg tacgacctgc cggcagtggc cacgctggtg ctgcgcgctg
    31921 acggaaaagg caacaacggc ttgaccatcg ccgagcggcg gtccatcgtg gagcgctaca
    31981 gcgaggtcac cgtgtatggc caggcgcatg ccggcggcag cggtgcaggc gagcgtgacg
    32041 gtcggcacaa catcaaagcc gtggttcgtg acgatggctt gactgtgtac cggccgaagg
    32101 ttgtggtgga ccacgaggcc atcaatgagg acatcgcacg tgcgcgcggt cgaaaaatta
    32161 tcagcgatgc gcggctaaag ggctatagcc tgatcgcttc gatcaacggg caccgtacag
    32221 aaagcggagt gctgtggacg cctggccagc gggtcaatgt caagagcgag ccccacggca
    32281 tcgacggcac cttctttctg atggcgcggc ggttagccgg cgacaaggcc accggccaac
    32341 gctcaaccct gacgctgaaa gaagacggcg tgtggacact ggacgcgcac ccgagcaagc
    32401 gcaagcaccg tcgcggcaag aacagcctgc caggccgcgt gctggacctg accacgggag
    32461 cgcccaaatg atcgatcaga tccgcaggga agtgacgcgc gggctgagcg gtgtgcgtag
    32521 cgcgttgcgc ggctttctgc acggcatcaa cctggcgcag cggattcagc gcgtggaggc
    32581 cgagggcttg gccggcgaag agctgccagg cttggagctg atgcagcagt tcggcttcac
    32641 gtccgcaccg cctgcaggct cgcaactgat catcattccg ctgggcggcc gcaccagcgc
    32701 atccgtcgtg gtggccacag aacacggggc gtttcgcttc aagctggaca accagggcga
    32761 gacggccatc tataaccagt ggggtgacct gattcattta cgtaaggaca ggtctatccg
    32821 cgtggtggcg gcactgaagg tggagatgga gacaccgttg cttaacgtca cgggcgcgat
    32881 ccatgccggc caaaacatca ccacggacca gagcgttgtt gctgccgtcg atgtgaaaga
    32941 tcagggtggc gcgaagagca tggcaggcat gcgagatatc tacaacctcc acgatcaccc
    33001 cgaaaccgag gtcacaaccc tccaacccaa ccaggcaatg tgatggacgc atggattgac
    33061 ccttcaaccc gcgactacgt cagagatgcc aaccgcgtcg gcgagttgac gcgcgcgcca
    33121 ggagatggtc tggccaatgc gatctacctg cgcctgatga cgccgctggg cagctggttt
    33181 ggcaatgtgt cccttggctc gcgcctgcat gagctgtcca gggagaaaga cctggcgcgc
    33241 gttgagcgcc tagctcagca gtttgcacgt gtggccctgc agccgatgct ggccgatggc
    33301 cggatccgct cgctcgacgt ccacaccgtg cgccctggag acggccggtt attgctcgct
    33361 gtggcagtgg tcgatgccca gggcgtggcg tccacgtttg aagtgcctgt caaggtcgtt
    33421 taaagaggaa taaatgtcta cgttttctgt acctgtcttt gagcagatcc gcgaccgcta
    33481 cctgcaggct gtcgccaacc aggaccccaa agccgccatt ggcccggaca gtgatcactt
    33541 tgtgcgcgcc agcggctatg cggccgtcac ggaaggcgtc tacgcccacc aagcttgggt
    33601 gttccgccag gcctaccccg acctcgccga ctcggacaac atgcagaaga tggccaggca
    33661 acgcaagata acgccacggc ctgctgttgc cgcgaccggt accgttcgtt tctttggcgc
    33721 agcgggcacc ctgatgccga taggccaaca ggccacgaca gcgcagggcg ttgtcgtcgt
    33781 atccaccgcg agcggtgctg tcggtgttgg gggcacggta gatctggcaa gcgcggccgc
    33841 tatagccggc gctgccggca atttgagcgc caacacagca gccacagtcg attccccgcc
    33901 tgatggagct acggccgctg cgaccatttt gaccatgacg ggcggcgctg atgtcgagga
    33961 cgatgcgtcg ctgctgatgc gcctgctgct tgaactcagt gagatcgccc agggtgggaa
    34021 tgaggtggac tataagcgct gggccttgag cgttccaggt gtggaccgag tgcatgtgtt
    34081 tcccacgcgt cgtggtgccg gcaccgtgga catcatcccg cttccggcga ctggcatgcc
    34141 tgacatcgtg ttgctggcgg ccgtgcagac gaagatcgat agtttgcgcc cggtaggcat
    34201 gctgcccaac atcggggctc tggtcattgc tcccacggaa gtgataactg ccgtgacagc
    34261 agcgcttgcg ctcgcgccag gtgcaactct cgccgtggtc gagccgctgg tggaggcggc
    34321 gattgccaga gtgtttgcgg cgcttgagcc gggcgacacg cttgtccgtc acaggctgat
    34381 cacggccatc ctgaatgtgc cgggggtgac cgacgtcacg ctggcagcac ctgcagccaa
    34441 cgtgacatcc ttggtggacg ccacacacct cgaaatggtg acgcaaggcg tcacctccat
    34501 cacctgatcc atgaacacgc gcgacgccct tattgcttgc ttacccccgc tggcgtatga
    34561 ccgccaagcc cccggcgtgc aggctgaggc gacggcggcc gcggcgattt tggacgatgc
    34621 gatcacgtct gccgacgtcg tgcgcctgga gcaccagccg gagatggctt ccgttgcgct
    34681 ggtggactgg gagcgcaact acaacctgcc agatgactgc gtgggcggcc ttggagcctc
    34741 tatcgatgcg cggcgaaaga gtctgtccgg tcgcatctac ggccgaggga atttgtccag
    34801 gccattcctg attgagcagg cggcagatct gggctatccg ggctgcacga tcaccgagct
    34861 ggggccgatg tcctgcacgg atccgtgtga cagcccggtc aatggtgagg acttcattgg
    34921 cgtctggcgc ttgaacgtcc cggtaagcac agccatccgc acagccacat gcaccgatcc
    34981 atgcgacagc cagcttcgca gctggggcaa cacgcaactt gagtgcgtga tcaatcgccg
    35041 caagccagcc cacacggtgg caattttcgg ctacgcgcct tgaccttgaa aacggagaat
    35101 taagtggact acatccagag cagcaacaaa caggtagaca agttcgggcc cggtgcgcat
    35161 ggcttttctg ccggcaaccc tactggcggc gagcctgcca ctttctacac gcccgattgg
    35221 tcggacgctg tgcaacagga gcacatcaac gctataaagg ccggaggctt ggtcccatcg
    35281 ggagcctcgc tgtcgcaggt ggcgcagtcg atcaggcgca tcgctggcgg caacatgacc
    35341 gtcgtcaatg tagctaacag cccctacgct ctgaccgctg acaacgccgg gctggtgatc
    35401 atggacgcca ccgctggcaa tatcgcagcg accctgccgg ctgttaacgt cgttacagcg
    35461 ttgccagtga ctttcaattt tgtccggatc gacgccactg gcaacacagc cgccgtcagc
    35521 cgagccggag ccgacacatt cattggtgga tcgacaggtt ttacgctgct ggggcaaggt
    35581 gatacccgct ctatcaaagg cgataccaca agcaagtggc tcactgtcgc gtccaacacc
    35641 gggagatcgc cgggcgacat ctttctgcac gccggtacta cggctccagc cggatcgctg
    35701 gtttgcccaa catctgtcac caatgtctca cggacaactt acgccagact gtttgcagcc
    35761 attgggacca cgtggggtgt tggtgatggg gcgacaacct ttggcatccc gtggttaccg
    35821 gcagactact cgctgctcca agccaactcc aatgttggta cgcagtctgt tggtgctgtg
    35881 atcgcccacg ttcacacaac cggcgtgaat agcatggcgg gcaacaacgg cggcaccggt
    35941 ccgggcacct ctgaaattcc atcgcccggg accgtgacaa gcaccggcgg tgcggccaac
    36001 tatgcagcag gatcgcgcgt gctgatttgt gttcagtttt aaggggtggt agatcatgac
    36061 ttcaaaaaca gttttcctgt acgacaaaga tggcgaattt aaaggccgct atgaggctca
    36121 agagagcccg ctagaaccag gcgtgtatat ccagccgacc agttcgaccc gcatcgcgcc
    36181 gccagttacg ggcccaaacg aggtggcgat tttcagcgct ggtgcatgga cggtgcagcc
    36241 tgactaccgt cgcgctgagc tgtacgccac agtcgatggg tctttaaaaa ctgtcgattc
    36301 aattgggccg ttgcccgtgg gagtgacgga caagccgcgt ccgtcgaaaa accacgtctg
    36361 gcagggcggt gactgggctg ttgaccttgc gcaactcaag gcttccaaaa acaccgagat
    36421 caacgcatgg cggctcaaag ccaaccgcag cacgttcgct cacggcggca aggtgttcgc
    36481 ttgcgatgaa ttgagccgaa gtgacatcga cggcgcgaac ggtcagatca gcaatctagg
    36541 cgcgctgcct gcgggctggc caggcggctg gaaggcggtg gacaacacct acctgcccat
    36601 cacgacggtg gccgagtgga aggcgtttta cggcagcatg tttaccgctg gcgccaccaa
    36661 cttcgcacgc gcgcaggagc tgaaagcaag acttgccgac gccaccacgc cggagcaagt
    36721 cgcggccatc gtctggtgac ccgtgatgaa gcttgtcttc atttacggca atcgattcaa
    36781 ttcccggctg accaagctgt tcaccgactc gacgtgctac cacgtcgggt ttacggacgg
    36841 cgtcaagttt tgggacatga acttgatcag acgtcgccgc ttgtggccgc tgtacccggc
    36901 tgagcgcacc atcgtcgtcg agtgcccggt gcccatgacg gctgagtact tggaccgcca
    36961 gctggacacc gatgagtcgc attacggcgt gctggactac ctgctgtttg gcttgcgcgg
    37021 gctttaccac ctggtcggca aaagcacgcg caatgcgggt ggggtgatct gcagcgagat
    37081 ggtggccaat gacctggcgg ccaacggctg ggaccggcgc tttgtcgagg tgccgtcgcc
    37141 cgccgatctg gagctggtga ttttggggag cagggacgcc ttgagggcca agcagctgga
    37201 ggccgcgtcg gtcgcgggct gatcaaaaaa agacagggcg aaggcctgcg gtgcgtcaac
    37261 acctcaagcc cccgcctcct accgtgaatg cacacggatt cgaccgaaga ccctgccacc
    37321 tgtacaggcg gggccatcat aagggcgatc catgcaaccg aagtttgagg taattaggtg
    37381 cggtcattgc caccgcaagc tgggagaagg caccttctcc cgtttggaga tcaagtgccc
    37441 gcgctgcaag gggcacaaca gtttgagagc cagcgcagct gatcacagca gcccgagtac
    37501 cacaccagca cgccaccgag cgtcatctga agacggaacc aaagatgacg acaaatcggg
    37561 agcggctaaa gctctttaac aattcaggcg accaggacat ggtccaggcg gcaccgatgg
    37621 tgcagtgggt gggcggcaag cgccgcctgg ccccg
//
LOCUS       DAIDFG010000003        30150 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_3_length_30150_cov_17.720053, whole genome shotgun sequence.
ACCESSION   DAIDFG010000003 DAIDFG010000000
VERSION     DAIDFG010000003.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 30150)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30150)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..30150
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3_length_30150_cov_17.720053"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         5314 a         9862 c         9647 g         5327 t
ORIGIN      
        1 ccgagggggc gctgcgcctg cggcccggcg aagccggttc cgcggcccct gcttggacta
       61 caactttttc tcccttggcg gctcggtgga tatttgaatg aataacgcaa aaaaattgat
      121 ccgcatcacc tacatcgggc tgttgatcct ggcgctggtg gcgcccctgc ttgggctcta
      181 tccggtgttt gtcatgaagc tgctgtgctt cgccattttt gcctgcgcct tcaacctgct
      241 gctgggtttc accggcctgc tgtctttcgg ccatgcggcg tttttcggct cggccgccta
      301 cgtcaccggc tggatcgtca agtcgcaggg ctggacgcct gaactcggca tcatggccgg
      361 tgtggtggcc tcgggcctga tcggcctggt ggtcggcatg gtcgccatcc ggcgccaggg
      421 catctatttc gccatgatca cgctggcgat tgcgcaaatg gtgtatttcg tctgcctgca
      481 ggcaccgttc accggtggcg aggacggcct gcagggcgtg ccgcgcggtg cgctgttcgg
      541 cctgttttcg ctggagtcgg acaccgccat gtattacctg gtggtggcgg tgtttgtcgg
      601 ctgtttcctg gccatttcgc gcatcgtgcg ttcgccgttc ggccaggtgc tcaagatgat
      661 ccgtgaaaac gaaccccgcg cgatttcgct gggctaccag gtggaccgct acaagctgct
      721 ggcctttgtg ctgtcggctg cgctggccgg cctggccggt tcgctgaaaa ccctggtcat
      781 gggttttgcc accctgtccg acgcacactg gtcgatgtcg ggcgaagtga tcctgatgac
      841 gctgctcggc ggtgtcggca ccttcttcgg cccggtgttc ggcgccggca tcgtgatctc
      901 gctgcagaac ctgctggccg acaaggtggg ctcgtgggtc accgtgatca tcggcgtgat
      961 ttttgtgctg tgcgtgctgg ccttccgcaa gggtgtggtc ggcgagttgc aggcttatat
     1021 ggagcggcgc cggcgcgctg cggccgcgcc cgacgcgtga gcaactgaag gctccggcaa
     1081 gtccggtagc cctcgcctag ttgtctgccg ctccgtcggt agacagcgcc tcggccacat
     1141 gctggggcac caccagctgg ccctgatcat cgggcaaggc ctcgatgctt atcaccggcg
     1201 ccgacgagct cacaccgcca aacatcgttg caaacgcggc gtctgccgca tcgcggccgg
     1261 tggccaggcg cacaaaaccc atgggaatcg ccgtgcccga ggggtcgaac atgtaccagc
     1321 ggtggcccac ataaacctcg acataggcgt ggaaatcggt cgacccgagc gccgggtcgg
     1381 cgccgtagtc gatgccgctg acaaaccgcg ccggaatgtt gaccgcgcgg cacagcgcaa
     1441 tcatcaggtg cgcaaaatcg cggcagacac cggcctcgtc gatcagcgtg tcgatggccg
     1501 tggtgttgcc ggtcgagctg ttggacatga aactcacgcg ctggttgacc cagtcgcgaa
     1561 tcgcctggac ccggccgtaa ccctggcgca gctggccaaa ctccttgacg gccagccggt
     1621 gcagcctgtc ggactggcaa taacggctgg ggtagaggta aggcagcacc gcgcctggca
     1681 gttgcaacac gccgacctcg ggcacctggt cgggttgcgc cacatggtgg cggatgtcga
     1741 ccgtcgcgcc gtagctgacc gtcagcggac cgggcatggc cctgaacttg aggtagcggt
     1801 tgcggctgac cgcgtcggta taaaactcgg gctgcaggac ctggctgatg cccagggttt
     1861 cgctgaccac cgcctgctcg cgcgtgtggg ccgcatggat gttgaagatg aaatcgcagc
     1921 cggcatcggc gacctggtaa cccagttcaa tggaaaattt aagcctgacc atatgttgtc
     1981 ttcctgaagt gggaacgcag ccagttgaag cctgttgcgt gtgctgcgac aagacgcagc
     2041 tgaagctgaa gcggacgggt tgccgacacg gcaacaggcg cgttgtggcg caactgagaa
     2101 cgattgtgaa cctgaaaaac caccctacgg gcaactcgag ccaagcggcc accgcaccgg
     2161 cgccagtggc gcatctcccg ccagggtcag acctgaccca gggaacgggc gttttcctac
     2221 agacggcacg caaaacgcct gggccaatct gcacagggtc tggccagcat ctttttcgct
     2281 attattttga tagcttattg cgcccgaaca ggaagggctg cgaccacttt ttcctcaaga
     2341 actcgaaatc agccacgaaa ttcacgccgg taggtcgaag gcgatgtgtt cagcgaggcg
     2401 gcaaagtgtt gacgcagtga cccggccgtg ccaaagcctg catccaccgc gatctgctca
     2461 atgctgcggc tggtcgactc cagttgccgc tgcgcaagcg ccaaccgctg attcaacagc
     2521 cactggccca ccgtggtccc ggtgacctgc cggaaatgcc gcgtgaaagt acgccgtgaa
     2581 agcagcgcgc gttgcgcgag ctcatccagg ctgtgcggcc ggttcaggtg tttttgcacc
     2641 caggccagca catcggccag gcggtggtcg gcgccggaag cgggcagcag ctgctccaca
     2701 tactgcgcct gcccgccctg gcggtggggc gccaccacca tgcgccgcgc catgtggttg
     2761 gccacctcgg caccgtgatc ggtgcgcagc aggtgcaggc agcagtcaat gcccgcggcc
     2821 gtgcccgccg aagtgaggac atcgccgtcg tccacgtaaa gtacatcggg ccgcagctca
     2881 acctgcggat aacgcttggc aaaagtgggc gccagcgccc agtgcgtggt ggcaggccga
     2941 ccgtcgagca gacccgcttc ggccagcaca aaagctccca cacacaggcc cacgatgcgc
     3001 gcgccgcgcc ggtgggctgc gcgcaaggcc ttcagcaagg cctcgggcgg cgcccggtag
     3061 ttcttctcgt tcgggctgtg ccatgagggc acgatgacaa tgtcggcccg cgccatgact
     3121 gagagcttgt gcacaccttg gaccgaaaaa ccggccggtg tgtgcagtgg gccggcctcg
     3181 gtcgcacata ccagcaactt gaagcgcggc atatgggcgc cccccatttc ctgcccaaac
     3241 gccagacagg gcaccgagag atgaaacgaa ctgatgccgt cgaaagcaac aaccgccacg
     3301 gtttgcaccc ggccggtttt gggggaagaa gcacgcatgg cccgattcta tcgatttatg
     3361 tcaattgggc cactttcgcg ccaagcgcat gaagcgaaca atccatctca tctatcaact
     3421 gcatcaagga gagcttccat gaccatcgcc acctcacgcc gcgcactcgt tgtgatcgac
     3481 gtacaaaacg agtacttcac cggcaacctg ttgatcaagc atccaccggt gcagcaatcg
     3541 ctacccaaca tcgcccgcgc catggatgcc gcccgcgcgg ccggcattcc ggtggtcgtg
     3601 gtgcaacaca gcgaggccgc cggcgcaccg ctgttcgcca agggctcctc cacctgggaa
     3661 ctccacgagg tggtgggcag ccgaccgcgc gaccactaca tcgaaaaagc ctggcccagc
     3721 gttttcaccg gcaccgattt tgcggactgg ctgaagcaga accgcatcga caccttgacc
     3781 gtgatcggct acatgacgca caactgcgat gcctccacca tttttgaagc cacgcatcgc
     3841 ggtctgcagg tggagtgttt gcaggacgcc accggtgcct tgccctatgc caacacggcc
     3901 ggcagcgcga cggcggaaga aatccaccgc gtgttcagcg tggtgtttca cacgcgcttt
     3961 gcagccgtag cctccaccga cgaatggatc agcgccgtgc aggtcggcca ggcactgaag
     4021 aaaggcaata tcctggtgtc gaaccaggcg gcgcttgccc tagcgtgata tcccgcgcgg
     4081 cgcgggcttg cggtggtcag aggcactgct tctgagcacc cgaccattca cttcattttt
     4141 gatagctggt gacgcccgcc catattgggc tggcagcaca tttcacaggc aaccggactc
     4201 aagcctcgtc catgaagcgc accttgacgc tcttgccctt gacgccgccg ttggccagct
     4261 tttgctgggc ctggctggca atcgcgcggt ccacggcgac ataggtggag aactcattga
     4321 cgttgatctt gccgacctgc tcgcgcgtaa acggcggcac attgccggca tcgccggtca
     4381 gcgcgcccag cacgtcaccg gcacggattt tttccttgcg gccaccgacg atctgcaggg
     4441 tcaccatggg cggcagcaac ggctcgttgc tggccggggt cagctcgctg agcttgtgcc
     4501 attcggactc ggccttctgc agcacctcga tttttccgac gtgacccatc tcgtccatgc
     4561 tggccaggct gatggcccag ccctcttcgt cggcgcggcc ggtgcggccg atgcggtgga
     4621 tgtgcacctc ggcatccggc gtcacgtcga cgttgatgac catttcgagc tgcgcgatgt
     4681 ccaggccgcg cgccgccacg tcggtggcca ccagcaccga gcagctgcgg ttggcaaact
     4741 gcaccagcac ctgatcgcgc tcgcgctgct caagctcgcc aaacaacgcc agcgcgctga
     4801 agccctggtc tttcaaatag gccacgagcg cgcggcactg tgacttggtg ttgcagaagg
     4861 ccagcgtgct ggccggacgg aagtggcgca acacctgcgc gacggcgcca aggcgggctt
     4921 cgtcatggtc gctgtcgggc acctggtacc agcgctgctg gatcttggtt ttttcatgct
     4981 gcgcctgcac cgtgatggtt tgcggcgtct tcatgaactg ctggctgatt ttcgcaatgc
     5041 cttccgggta ggtggccgag aacaacaggg tctggcgctc tttcgggcat tgtttggcga
     5101 cggtcgcgat gtcgtcgaaa aagcccatgt ccagcatgcg gtcggcttcg tcgagcacca
     5161 acgtgttcat ggcttccagg ttcaggttgc cgcgtgccag gtggtccatg atgcggcccg
     5221 gcgtgccgac gacgatgtgg gcgccgtttt ccaggctggc ggtctggttg cgcagcggca
     5281 caccgccgca gagcaccacc accttgacgt tttcctccgc ccgcgccaga cggcggattt
     5341 cggtggcgac ctggtcggcc agctcgcgcg tcgggcacag caccatggcc tgcaccgcaa
     5401 aacgtcgcgc attgaggttg gccagcaggg gaatggcaaa agcggcggtc ttgccgctgc
     5461 cggttttcgc ctgcgcaatc aggtccttgc ccgccagagc cagcggcagg ctggccgcct
     5521 ggatgggcgt catggccagg tagccgaggg ttttcagatt ggcctgcatc gcagggttca
     5581 gcggcagcgt gctgaagtca gtacttgctg caccggaagt cgggagggaa gaagtcattc
     5641 cccgattatc ggcgctagga gtctgcgcgg tagaaggcgt cgaagcgaaa agcggcccca
     5701 gggaggacag tttttgcctg atttgcagcc ccatagccgt cgctatgggg caataagccg
     5761 gtgaaaaatg gaccgctgcg gtcgctttgc agccgacgat ccttctgccg cgcagactcc
     5821 tagggctttt caagcccgat ggcgcacctt gagagccctt ggcagacgca ggcagcgcca
     5881 gcagctcagg cgataaaaag atgaatgaaa acagggtcca gcccttattg aacgtgcgcc
     5941 attagctact ttattgatag cgtttccagg tctgccagcg tcggtgccgc cgacaggggc
     6001 cgcagacgcc cgcacagcgt aagcttgccg gaacacctgt tcttttttgt ccgctgtcac
     6061 cctcccaccc gccatgcctt ttgctgatac ttccgccgcc gtggtccagg ccctgggcca
     6121 ccagggcctg acgctctttt ttgcgctgct ggccttgctg ctgcccaccg ccaccctgct
     6181 gtggtggctg gcgcagcgct acgcggtgcc aggcaagacc agcacgctgc cggccggtgc
     6241 cttcctgctg atccggctgg catcagggtt tgccgtgctg gtgggcgcag cggccatctt
     6301 tgctgaaatt gccgaagccc tgggtgatgg cccacgcgcc ggccagctcg accagctgtt
     6361 ttcagacgcg cttcacgcca gcaccgccgg gtgggcccag cagctcttcg cgtggatcac
     6421 ccggctcggc gacaccgcca ccctggcggc atggggtgtg ggggtggcga cggtgctgct
     6481 gctgcgcggc cggcgctggc tggccctggg ctgggtgctg gccatcagcg gcaacgcctt
     6541 gctcaacacc acgctcaagg cgctgtttga aagaacccgg cccgtacacg acagcgccct
     6601 ggtgcacgcg gacggctgga gtttcccgag cgggcacagc tccggctcgg tggtggtcta
     6661 cggcatgctg gcctatgtgc tgatccacag cctgccgccg gccatggcgc ggcgcgccag
     6721 cctgccgctg gtgctgctgg ccgccaccct ggcgttttcg gtcggttgca gccgggtttt
     6781 tctgcaggtg catttcgcca ccgatgtgct cgccgggttt gcctcgggga cggcgtggct
     6841 ggccgtctgt gtggccagca tcgagctgac gcgtcactat ggccgcaccc gcgccaccgc
     6901 cctgcggtag agcgcaagcc cggaggaagt catcgccggt caccggcccg gcatctgaaa
     6961 cagcgcttca gtggtggcag aacaccggcc ggcgcttttc aacgaaggcg gccgtgccct
     7021 cccgcgcatc ctgcgaagcc gccgcaatca tgaactgcgc cgcctcgatg gccaggccct
     7081 cgtcgatgga cacattgagc ccgcgcgtca cggcgcgcag cgaggccgtc acggccacgg
     7141 cggagttctc ggcgatgcgg tctgccatct gcaggcaggc cggcagcagt tcatgcgccg
     7201 gcaccacgtg gttgacgagg ccgagcaaca gggcttcctc ggcgctgatg gcgtcgccgg
     7261 tcaggatcat ctcgttggcg cgcttgcggc cgatgtggcg tggcaggcgc tgcgtgccgc
     7321 caaagggtgg cggaaagcca agccggattt cgggtttggc aaagcgcgca tggccggccg
     7381 cgatcgcaag gctgcaggcc tccatgatct cgcaaccgcc accgaaggcg atgccgttga
     7441 tgcccgcgat gatgggcttg gggtagttct cgatgcgccg cgtcagggcc tggccgcgca
     7501 tgacgaagtt gcgccaggcc gcctcgggcg atgcggcgac atcgggcgca aagcccgcga
     7561 tgtcagcccc tgcgctgaag gccttgtcac cctgccctgt cagcacaaga cagcgcacgc
     7621 tgtcgtcgcg ctccaaagcg ttgagtctgt ccatgagttc gtcgatcagt tcataactca
     7681 gcgcattgag tttgtcgggg cggttgaggg tgagcaccac ctgttggcca tggcgtccgg
     7741 tttctatcag catgttgtct ccagtgggtt tcgggcaggg gtggggaggg ataaaaatga
     7801 caggacgccc caaccgcgcg gcgcccccac aaagtaggtt gcggcttgcg gcgtgacaaa
     7861 ggactggccg ctctcgagca cgatgtcgct gtcgcggcgt ccggccaagg catcgctggt
     7921 gatccagagg caacccccgg tgcaggtcag gacctggccg cgccgcaggc gcaggggttt
     7981 cgggatggac ccgagcgtca acggggtttg cgaaataagc atggcagcct ccttgaaaag
     8041 tgtggtttga ttcttccctc gtcccggccc gcgcggccgg atggtttgtc atccggggta
     8101 ttcctgtcat tcatgccatg ctgagtcctt cttcttccca tgcgctgcgc gccatgtcgc
     8161 tggacgcgat acgcggcttt gaatcggcgg cacgccacct gagcttcacg gcagcggccg
     8221 aggagctgtg catcacgcag tcggcggtga gcaagcagat caaaaccctg gaagacgcgc
     8281 tgggcaaagc gctgttcctg cgcggcggca agggactgag cctcacgccg cagggccgcg
     8341 agctttatga ggcggcccgc gcggcgctgg cgcaactggc ctcggccgtg gatcgcctgc
     8401 tggcggtcga ccgggcgtcg gtggcggtca ccaccacgcc ctcattcgct gcgctgtggc
     8461 tggcgcccaa actcgcaaaa ttccagcagc aggagcccgc cattgacgtg cgcgtcgacg
     8521 cttctgaagc ccgggtgaac ctggagcgcg aaggcatgga cctggccgtg cggctgaggc
     8581 ctgtgttgcc cactggcgaa ggcccgccgc ccctgttgca ggaacaagcg ctgctggtcg
     8641 cggcgccggc cgttgcggcg cgcatccact cggccaccga tatcctgcac acgccgctgc
     8701 tggtgtacca cgacccggcc atgcgctttt cgtggatgtc ctggacggcc tggtacgagc
     8761 gcctgggcct gcctcaatcg gcccggcagc cttgcctgta tttttcgcag tacgaacatg
     8821 tgttgacagc cgccgtgcag ggcgccgggg tcgccatcgg gcgcacgccg ctggtgctgc
     8881 cactgctggg cagcggccaa ttgaacgtga tcctgcccgg ccagacggtg gccggcctgg
     8941 gctaccacat cgtcacgtcg gcccacagtg cggaacggcc agcggtgcaa aaattccgcg
     9001 cctggctgga gcgcgaactc gcgctggaag ccatcggctg aggctgttca acgagcgggt
     9061 cggtcccaag cgcccgtacc ctcgagcacg ggagacaact ccgccttgct ccagcgcagg
     9121 ggcaaggacg attccaccag gcgtatcgac tgtacctcga aacgcatcaa gcgctgcgca
     9181 ccggcgaagg atgacaactc ggggccgtcc cagagaatgc cggccgtgac ggtcaggtaa
     9241 agcaggtcgc ccttgtcgaa atcaatgaac agcagcccgg cgcgcgggtt caccgcgata
     9301 ttgcccagcg tgttgaagaa aaagttgcca gtgaagtcgg gcaccgtcag cacgccgccc
     9361 tcactgaggc gcacaaagcc aggcttgcca ccgcggtgcg acacgtccac gcccgcggcg
     9421 cgacccgcct gcatgctgtc accggcgtag gcggtggcga taaaaaacgt gtcggcgctg
     9481 gtgatgatgc ggcgcgccgc gtcgtcgagt tgcaacaatt cgtgcaccac cggcacggcg
     9541 ccggcgctgt cttcgacata caccggttca cgtgcctgga tgtacttggg gcagttgccg
     9601 aaactctggc cgacgtccac cgaaaaccct gaagcgcctg catggcgcac cacgccgttc
     9661 atgcggttgc gccgccgtgt gtgcggctcc agccccagca ggccgatggc cgcgccttct
     9721 gccaggttgg cgaccagcgg atcaccgggc agcggccgcg cgctgaagtc cagctgacgc
     9781 gggtggggtg agtgcatgaa gcccggcggc tgcgccagca ccgaagccca gggctggccg
     9841 cgcgcatcca cggtgccggc gatcacaaaa ggcagctgcc cgaaaaatgc gcggtgctgg
     9901 tccggcatga agtcgcggat gacacgcggg ccgagctcgg cgagccgctc ctgcacgccc
     9961 acacgcgcct gcacctcacg ttcgccttcg tgaaacgcgg gccgggtcat gaaatcacct
    10021 ctgcggtatt caccccggaa acaatcctgc gtttcatgct tccagcccaa tgggggtctt
    10081 caccatcggc acaaaacccg gcaaggcctc aatacgggcc agccaggcgc ggagcgctgg
    10141 atagggttcc agcgacacat tgccttcagg cgcacgcgcc acatagctgt aattggcgat
    10201 gtcggccagc gtcggcgcgc tgccgaccag aaagggggcg tgggccaagc cctgctccat
    10261 cacctcgaac agggcatggg cacgtgccac tgcttcgaca ttttccggcc gtttgaacaa
    10321 ctggctgacg cgtgccgccg ccgggccaaa agcgagttgc ccagcggcga ccgacaacca
    10381 gcgctgaacc ctggcggcac ccagcgggtc acgcggcaac cactggtgat cggccccagc
    10441 ggcatatcga cccgcaaggt acaccaagat ggcattggag tccgccaggg tgacgtctcc
    10501 gtcctgaatc accggcacct gaccaaacgc attcatggcc agaaacgccg gcgctttatg
    10561 ccccttggcc atcaggtcga tctcgatcaa ctcaaaaggc agcttgagca gggacaggaa
    10621 aagttctacc cggtgagagt gcccggaaat ggcgacgcgg tagagtttga tgggctgcgc
    10681 gggggaggtg gtgttgcttg tgctcatgca tgtccttcga aaatgagttc aggtgctggt
    10741 attgtgtttg atccattaat ttgaataaat attcgaaaca ggatttcact catccaaaat
    10801 tcggagtaat tcatttagca tggacccatg gacaaattca aggccatgca ggcttttatc
    10861 cacatcgcgg aagaaggcag cctgaccgcc gcggcacggg tcatggaatc gtctttacca
    10921 gccatggtgc gcactcttgc cagtctcgag agccacctgg gtgtgcgact tttcaaccgc
    10981 acgacccggc gcatcgcgct cacagacgag ggacgccgct acctgatttt ttgtacgcag
    11041 gtactcgcaa cggtgcagga agccgaagca tcggtaagcc cccagatggg ggagccacgc
    11101 ggccagctcg tcatcaccgc gccggtgttg tttggacaga tgtatgtggc gccggcggtc
    11161 acccgatttg tacaacgcta cagcgaggtg agctgccggg tgatgctgca cgaccgggtg
    11221 ctcaatctcc tggaagaggg aatcgacgtg ggcatccgca ttggcgagct ggaagactct
    11281 tcactggtag cgcagaaagt cttcgggata cgccgcctgg ttgtcgccag cccggactac
    11341 ctgcgcaaac acggtgtgcc ggcacatccg cgcgacctgc ggtctgccaa ttgcgttcgg
    11401 tttaccgcgc cgacgggccc gtggtggact ttccacgaaa agggtaaaac cttcacggta
    11461 cctgtcaccg gcaatctcga attcaacaac gctgcgcctg cggtcgaggc ctgtgttgca
    11521 gggatgggtt ttggcatgtt tatctcgtac caggtcgccc cttatgtgca gcagaaaagg
    11581 ctcaccgtgg tgctcgacgg ttttgagccg ccgctgcgtc cggtcagcgt ggtttatccc
    11641 cattcacgcc ttctgccagc gcgcacgcgg attttcattg agtggatcaa ggccgaactg
    11701 ttgaagctca atcacgctgc cccgcctcag ctgcatccgg aacgatgacc ccctccgggc
    11761 attggctgcg gaccggtggt aaggtcagtg ccaacccttt ccaatgatgg caacccatga
    11821 aaaatttcga ctttgatctg ttcgtcatag gcggtggcag cggcggcgtg cgcgccgcgc
    11881 gcatggccgc gcagcgcggc gcacgtgtgg cgctggccga ggtagcagcc atgggcggca
    11941 cctgcgtcaa cgtcggctgc ataccgaaaa aactctacag ctacgcggcg cactacgccg
    12001 aagcctttga agaatcgcgc ggcttcggct ggaccggcga agcgccggtg ttcaactggg
    12061 agcagctcaa gaccaaccgg gcgcttgaaa tctcgcgcct gaacggcgtg tacatccagc
    12121 tgctgcaagg ctcgggcgtg cagatcatcg agggctgggc caccctggcc gacgcccaca
    12181 cggtagaggt ggaaggcagc cgctacacgg ccaaacacat cctgatcgcc accggcggcc
    12241 ggcccagcct gcccgaggtg aagggccgcg agctggtcat cacctcggat cagattttcg
    12301 acatcgctcc tttcccggcg cgactgctgg tggtgggtgg cggctacatc gcctgcgaat
    12361 ttgcctccat cttcaacggc ctggggtcca aggtcaccca gctttaccgc ggcgcccagg
    12421 tgttgcgcgg cttcgacgcg gaagtgcggg ccttcgtggc cgcagagatg atcaagaccg
    12481 gggtggactt gcggctgcac gccgatgtgg cagccatcag ccaggcggtg gacgggttgc
    12541 acgtgcaact gcaggacggc agcagcacca cggtggacgc cgtgttgtac gcgacgggcc
    12601 gtctgcccaa tgtggccggc ctgggtctgg aaaacgcggg cgtcccgctc aaccaccagg
    12661 gggcgatcga ggtcaatgcc cactaccaga cctcggtgcc gtcgatctat gcgctgggcg
    12721 acgtgaccgc acgcatccag ctgacgccgg tggcgctggg cgaggccatg gtggtggtcg
    12781 accacctgtt tggcgccgcg gccggcaaga agccgcgcca catgagttac gacttcatcc
    12841 cgaccgcggt gttcacccac ccgaacattg gcaccgtggg ttattcggaa gccgatgcac
    12901 gcaaggaatt cggcaaggtc agcatctacc gcagcgagtt caaggcgctc aaacacaccc
    12961 tgagcggcag caccgaacgc acgctgatga aactggtggt tgatgacgct tcggaccgcg
    13021 tggtcggctt gcacatggtg ggcgccgacg cgggcgaggt ggttcagggg tttgccgtgg
    13081 cgatgaaggc cggtgcaacc aaggccattt tcgacagcac catcggcatc cacccgacgg
    13141 cggcagagga gtttgtcacg atgcgcgagc cggtgaaaga gccgtcgcgt gcgccctgag
    13201 cagcctggcc ctcacgccgg tggcgcgttc tcctcatcgg gaaggaccag gctggcgttt
    13261 tcaatcgccg catcaaaggc ggcaagttcc gcatccccct gaaaagcggg gaggctgtcc
    13321 aacgggtgaa acacctggtg ttgcagccgc cgcagggcgt cagacttctt caccgcctcc
    13381 agcacctttt ggggcgtcat catcaacatg aaaggattgg cgctgggggc gtcgacttgc
    13441 atggcagcag ctccttgtac acatgggtgg cacgtgaacg acaaaacaga tggctgcact
    13501 ttaggagacg ccgaaaattc aggaaagtcg gccggcagcc cgatcacttg taggtgaccg
    13561 gctgacacgc cccccggcgt accggcacgc tcagaatgcg cgcacgccct ggtgccaggc
    13621 cgctcgcacg atgggcatgt caccgctcat ccggacccgg atcagatcgg cgcgcaggcc
    13681 gggggccagc tcaccgcggt ccgtcaggcc aatcgcacgg gccgggttgc gcgtgacggt
    13741 gttgaccgcc ttgggcaggc tccagccggc cgtatcgcgc agcaagaagg ccgactgcag
    13801 caggctggcg ggcacgtagt ccgacgaaaa aatgtcgagc aggtcgagct gcgccagttc
    13861 ggccgccgac acattgcccg aatgcgaacc gcccttgacc agattcgggc cgcccatgac
    13921 gatgcccatg cccgcctcgc gggccgcccg ggcggcggcc acggtggtcg ggaattccga
    13981 gatggcaata ccttcctggc tggcctgctc gacatgcgcc acttccgtgt catcgtggct
    14041 cgccaccggc aaggcccggg cgcggcttcc ggcgatgacg gccgcgcggt tgggaatggc
    14101 aaaacgttcc tggtgttccc ggcgctcctg gaccagggcg tcgtattccg cgtcgttgaa
    14161 gctgccgtaa cgctcggcat aacgccggta cttgcccagg tcgcgccact ggcgctggcc
    14221 cggcgtgtgg tccatcacgc tgaccagttc gagcagagag tcgtcggcat agtgctcgaa
    14281 cacctccacg atatcggggg ccgagacctc acaacgcagg tgcagcaggt gctcgacccg
    14341 cagcagccct tcggcgcggc actcgtgcag ggcctcgatc gacacatgct gggtctggct
    14401 gcgcgtgccg ccctggtcca tgtcgccgat gacgaccgaa tcgagcaccg tgatgatgcc
    14461 ggcagccgcg cagagcgcgt cgtgcatgac cgtggcggaa tgggcgttcc acaacacgcc
    14521 gggccgcggc accaggtgtt tttcgaggtt gtcggtgtgc agctcaacca ggccgggcaa
    14581 cagccagtcg ccggcccagt cttccgcgcc ggtcaccgag gtgtcgccgc gctcaatgga
    14641 gcggatgagg ccgccctcga ccaccagaca gccgatgaac tcttcgtccg ccgtgacaat
    14701 tcttgcgttc ctgatgattt tctgcttcat ggggatcttc cggttcagtt tgattttcga
    14761 tagtgttcga catccaggca gcggctcgct accgcttcgc gcacttcgtc gtcgtgaaaa
    14821 atgccgacga cggcggcccc gcgcgcacgt gccgcatgaa tcagctccac cacgacgcgg
    14881 cggttgtcgg catcgagcga ggcggtcggc tcgtcgagca gcagcacggg gtggttggca
    14941 atcaggccat gcgcgatgtt cacgcgctgc tgctcaccgc cggaaaacgt cgcgggcggc
    15001 aagtgccaca ggcgctcgtg caggttcagg cgcgccagcc actgcgcggc ctgggcgcgc
    15061 gcctcgtcgg cggccacacc gaggcggcgc agcggttcgg ccacgatgtc cagcgtcgag
    15121 acccgcggaa tcacgcgcag aaactgcgag acataaccga gcgtgcggcg ccgcacatcc
    15181 agcacctcgc gcggcgctgc cgcggacagg ctgagccact ggtcctggtg gcggatgcgc
    15241 acctctccgc tgctggcgct gtaattgccg tacaggcagc gcagcagcga actttttccg
    15301 ctgccggaat ggccggtcaa ggccaggcac tcgccggggc cgacgctcag gctgatgccg
    15361 gaaaaaacag gcagcgccag gccgcctcgc gcgtgcaggg tgaaagtctt ggagagctcg
    15421 ctggtttcaa ggacagaagt catggtgatg ctcaggtggc gaatcaagtg gcgaatcagg
    15481 gggtgagaac ggaagacacc agcagctggg tgtagggatg ctgcgggtcg tcgagcacgc
    15541 ggtcggtcag gccctgctcg accacgcagc cgtctttcat gaccagcatg cggtgcgcca
    15601 gcaggcgcgc cactgcgagg tcgtgcgtga caatcacggc cgccagatgg agctggtcga
    15661 ccagctcgcg cagcagatcg agcagccgcg cctgcaccga gacatccagc ccggtggtcg
    15721 gctcgtccat gaagaccagg cgcggatggg tcaccaggtt gcgggcaatc tgcaggcgct
    15781 gctgcatgcc gcccgagtag gtgccgggca ggtcgtcgat gcggtccaca tcgagttcga
    15841 cacgttccat ccagctcagg gcctcgccgc gcagcttgcc gtagtggcgc gcgcccagag
    15901 ccatcatgcg ttcgccgatg ttggcgccgc ccgacacatt catgcgcagg ccgtcgcgtg
    15961 catgctgctc gacaaaaccc cagtcggtgc gggccagcca gcgcagccgc gcttcggaca
    16021 acccggccag atccaccagc ccggacgcgc cgcgtgatgc ggcgacgccg ttgccggcct
    16081 cctcccgcag gtcgtaatgg accgaccctt cgtcgcaggc cagacgcgcc gccagcaggc
    16141 gcagcagcgt ggacttgccg gagccggatt caccgacgac ggccagcacc tcgccggggt
    16201 agaggtcaaa actcacaccg cggcaagccc agcgcagacg ctggccctgc atgaactgct
    16261 tgccggcatc gcgtacggac aggagaagat cgctgctcat cgcgcgcccc ccaccgctgc
    16321 gcgcgccagc gcgtccggca tctgcacggc ctgctgcgtg cccacatgcc cgccctcgcg
    16381 gcgcccactg cagaagtcac tgtccgaaca gacaaacagg cggctgcctt catcgtccac
    16441 aatcatttcg tcgagaaaac tttccgccgc gccacacagg gcgcaggcat gttcccagcg
    16501 ctgcacttca aacgggtggt cctcaaaatc caggctgcgt acagatgtgt agggcgggat
    16561 ggcgtaaatg cgtttttccc ggcctgcccc gaacagctgc agcgccggat tcatgtgcat
    16621 cttggggttg tcgaacttcg gtatcggcga aggcgaggtc aggtagcgtt cgttgaccag
    16681 caccgggtag tcgtaggtct tggaaatggc gccgagctgg gcaatgtcct cgtagagctt
    16741 gacgttcatc aggccgtatt cccccagcgc gtgcatggtg atggtttcga ggcggcgtgg
    16801 ctccagccgg aacagcggct ccggcatggg cacctggtag acaatggttt gcgcttcggt
    16861 cagggcggct tccggaatgc ggtgccgcgt ctggatgatg gtggcctcgg cggttttctc
    16921 cgtggtcggc acgccggtgg tgcgcgcaaa aaatcggcgg atgttgaccg cattggtcgt
    16981 gtcgtccgca ccctggtcaa tgaccttgag cacatcatcg cggccgatga tgctggccgt
    17041 gacctgcacg ccgcccgtgc cccagccgta ggcgaacggc atttcacgcg agccgaaagg
    17101 cacctggtag ccgggaatgg cgaccgcctt gagcatggcg cggcggatca tctttttggt
    17161 ccgctcgtca agaaatccga agttgtaatg ctcgtccatc atgctgcttt ctgtatcgcc
    17221 ggttcggtgt ggggggtggg cgctccacgg ccggggtccg cgcgtttttc atcctgccct
    17281 tcctgcgcat ggacggcacg cagccggcgc accagttcca gctcggactg gaagtccacg
    17341 taatgcggca gcttcaggtg ctgcacaaag ccggaggcct ccaggctgtc ggcatgggac
    17401 agcacgaact cctcgtcctg tgccggcgcc acggccagct cgcccagttc gcgcgcacgc
    17461 agcgcgcgat cgaccagcgc catcgccatg ctcttgcgct cggccccgcc aaacgccagg
    17521 ccgtagccct gggtgaactg cggcggcacc tcgcggttgc cggcaaactg gttgatcatc
    17581 tggcactcgg tgacttccac ctcgccgata tcaatcgcga agcccagctc gtctgccaga
    17641 atttccaccg acacggcgcc gcgccggatt tcaccggcaa acggatgggc attggcataa
    17701 ccgcgctgcg tggaatagcc caggcccagc aaccagcctt cgtcgccacg cgccagggcc
    17761 tgcaggcgca gcggccggtc ggccggaaac atcagtggct cacgcgtcag gtcgcccggc
    17821 tcgctgtctg cgacgggacg cggtgtctcc atcaggcctt cacgcgccat caattcattg
    17881 acacgcggca tgctggccgg caagggctgc gccacgccct ggggcgccgg ctccgcctcg
    17941 tcgctgtccc cttcggccaa caaggcaaag tccagcagac gctgggtgta gtcatacgtg
    18001 gcgccgagca cctggccacc gggcacatcc ttgaacgtgg ccgagacgcg gcgtgtcagg
    18061 ttcatctgtg cggtgtccag cggcaggctg tagccaaaac gcggcagcgt ggtgcggtag
    18121 gcacgcagca ggaaaatcgc ctcgatcagg tcgccactgg cctgcttgat cgccagggcg
    18181 gccagttcgg ggtcgtacag ggagccctcg gacatgacac gatcgaccgc cagcgacagc
    18241 tgcgtgcgga tctgggcaac cgacagttcc ggcagcgcgg tatcaccgcg gcgctgcgcg
    18301 gcgagcaggt gccaggagtt ctcgatggcg gcggttccgc ccttgacggc tacatacata
    18361 tcaaattcct tccagacgtc gcacgcgggt cgtgcgcggc agccccaacg ccaggtccgc
    18421 gcaggtaaaa acaatgtcca ccccttgtgg aaacgcggcg tggttgactt gccactgcgt
    18481 ccagaagtgg tcgggcaagc ccgcaatccg caccgtctgc atgtcctgga tgccgggacc
    18541 acgccactcg acggccgggc cgtcatccag cgagggcact tccaccagca aggtggccga
    18601 gcgttcgggc gcttccgccg agcctggcgc aaagctgtcc agcggcggca tggcccccgc
    18661 atcgacgagc acggcaaaag ccgcctcttg cggcgcggcc gcccggcccg ccccggtgtg
    18721 aaaacgcagc cagtccgggg caccgccctc ctcgcgctgc caccataccg gcgtgtcgtc
    18781 gtccgtcagt gcgagcagca gatgcgccat ggccgggccc agggccagac ccggaaccgc
    18841 gccgccgaga acgaagcgcc ggcccggccg cgacagcgca tcgagcacac ggcgaaaagc
    18901 agtctgtgcg tcatgcaccg gatcggcgaa gccggcactc agtggctggt tctgattcat
    18961 gcctggcccc gcacaaacgt gaaaaattca actttcgacg cggccgccgc gcgactggtc
    19021 gcgtcccgct gcgcggcctg ttgcctggcc agcggctcaa ccaccgcatg catgatgtcc
    19081 gcgtggcgtg tggggtcctg cagcagcgca tcggccacgg caatcagctc ggccttgcgg
    19141 cggtcgcgcc ccagggcgta acccacgccc agcgcgcccg ggccgacgcg cagggcacaa
    19201 cgcgtcacgc tggcctcccc gagattgaag gcgtcgccgg tccccccgac ccggccgcgc
    19261 agcatgacca tgcccgattc ggcaggccgg acatgctcca gcggcggcag ggcgcccaag
    19321 agagcccacg ccttttcaag ttcgggccgg gtggcgcgtg ccagcacggc cagccagcgc
    19381 ctgcggggtt catggtttgg gtttatcgac tcgtttgtca tacagatgtc taaatcagtc
    19441 actagagttg ggtttgtcac tgcaaacaac cacaggggct gcaggaagac cctagttcaa
    19501 ccggacaaca tgaccaaatg atgacaagcg aacgccctct ttccagcgcg cccggccggc
    19561 gcagcggtgt ggctgtgtgg aagcagattg ccgacacact gggcaccgaa atccgtgacc
    19621 gcgccttcac ggcgaccggg cgtctgccaa gtgaaaacga gctctccaca cgttttggcg
    19681 tgaaccgcca cacgctgcgc caggcggtcg ccgccctgca gggtgatggt ctggtgcgga
    19741 tcgaaccggg ccgtggcacc ttcgtccagc acgaactgct cgactacgtg ctgtcgcgcc
    19801 gtacgcgttt cagcgaaaac ctccagcgcc aggggctgtt gccgagcaaa cagctgctca
    19861 ccgcccggga aatggccgca cctgaacgag cggcgcatga actccggctg ggcaagggtg
    19921 gcaaggtgct gatggtggaa atgctggacg aggccaacga gcagcctatc gcgctggcca
    19981 cggcctacta ccccgccggg cggtttgccg gcctgctcga catgcttggc cgcggcacct
    20041 gcaccacgga catcctgcgg cactttggtg tcgaggacta tgtgcgcgcc gaaagccgcg
    20101 tcaccaccca gatgccctcc gctgaaaccg cacggctgct gaagcagccg ctgacgcggc
    20161 cggtgctgtg cgtcgagtgc ctggacgtcg acatggccgg cctgccgatc aagtacgggg
    20221 aaaccgtgtt ttccggcgac cgggtccagc tggtgatcaa tgcaggaaat gaaacgggaa
    20281 cgttcgcatg agcgcacgtt acgccattta ttttgcgccg gaccggcact cgccatggtg
    20341 ggcctttggc gcgcactggc tgggccgcaa cgaacacgac aacacggcac tgccccagcc
    20401 cttgcaggaa ggcttgccac cagcggagtt tgccgccatc actgcggaac cgcggcgtta
    20461 cggcttccac gccacgttga aagcgccctt caggctgtca gctgcacacg acgaatccga
    20521 tctggtggca cgcctgggcc gcctggcgca gacgctggca ccggtgccgc tgggaccgct
    20581 gcgtgccgcc gcactgggca acttcgtggc gctggttccc gaggcccccc ccgagggcct
    20641 gcaggcgctg gcggcagcct gtgtggccgg gctcgacgac ttgcgcgccc ctcccgaggc
    20701 ggcagagctt gcccgccgcc gcgcgacccc tctggacgcc cgcgagaccg agttgctgaa
    20761 tctgtatggc tacccctatg tgatggagag atttcgcctg catctgacat tgaccggccc
    20821 cgtcgatgcg gcccttgccc ggcaggtcag ccaggcggtc gctgcggacg tggaccgcct
    20881 caacacccag gcccccctgg ccctggaccg gctctgcctg tttgtcgaac gtagcccggg
    20941 cgcaccgttt cagcgtgtca tggatgtcag gctgggggca tgagcggcct ctgggtgttt
    21001 gtctgcggac cgtccggttc cggcaaggac agcgtgatcg acgctgcgcg acaggcgctc
    21061 agcgggcggt ccaacatcgt gtttgcgcgc cgcatggtga cgcgccccgt gcagccgggc
    21121 tccgaccacg atccggtcac cgcaccggtc tttgccgaac tgctgcagtc gggcagcctt
    21181 agctggcact ggcaggcgca cggatttagt tacggtattg ccagccatta cgccgacgcc
    21241 gtcagggccg gatgtctcgt ggttgtcaac ggctcgcgcg cccatgtcaa cagcctgcca
    21301 ccttcatccg atcttcgggt ggtgcagatc agcaccgatc cggagcgcct ggccatccgc
    21361 ctggcgcaac gggggcgcga tagcgacgcg gccattgtcg agaggctggc acgcaacgct
    21421 actttctccg gcatgccagc cgattgcgtg atcgtcaacg atggagcgct gcaagtcgcc
    21481 ggccagcgtt tggctgacta cctgaccggt tcagccggga cggcagaggc ggcctgttcc
    21541 agaatcacgc ggtagtgggc ccaatcgggc ggtgtcgccg cagggtcttt ggcgtggtcg
    21601 tcccagcgcc gcagcgccac cgcatcggcg gcaaaagcct gcgccatgaa aacggcggcc
    21661 tcctctccgg aaaacggccc gccctgcagc gccagactgc gctgcgacgc cggtgacagg
    21721 ctggcctggt actggctgtc cacccggcag agatagcgct tggcgtccac atgcatgcga
    21781 atgggttcga gcacggcgtc tggaaataca ccgcgtaaaa acggcagcgc cagaaactgg
    21841 tgcaggtcat caacacctgc ttccttcgcc ggttcccgct gtactagcaa atggccgagg
    21901 tcgtgcagca gcgcggcagc aaccagttca ggcgaggcgc ccgaacgctc ggccatgtgg
    21961 gcgcactgca gcgcgtgctg cagctggttg atcgcctcgc cgccgtactg cgcggccccg
    22021 cgtgtctgga aaactgcggt gatctcttca attttcaatg ccatggctgt tcctagacca
    22081 gtgccttgcg tatgcgtgct gaaatcaggt cgatgacgct gaccgtgacc acgatgatga
    22141 tcatcacggc gcaggtttcg gcatattgaa agccgcgaat gatctcccac aacacgacgc
    22201 cgatgccgcc ggcacccacc atgcccacca ccgaagcgga acgcacgttg gactcgaagc
    22261 ggtacagcgt gtaggaaatc cagagcggca gcacctgcgg gatcacgccg tagaggattt
    22321 cctccagcgc atgggcaccc gtggcgcgta tgccctcgac gggctgcggg tcaatcgatt
    22381 ccaccgcttc cgaaaacagc ttggccagga tgccggtggt gtgaatccac agcgccagca
    22441 cgccggcaaa cgggcccagg cccacggcca cgatgaacag catggcaaac accatctcgt
    22501 tgatggcgcg tgccgcatcc atcaggcgac gaatgggctg gcgaacccag accggtgcaa
    22561 tattggccga ggacagcagg gccagcggca cagcacagac cacggccagg gccgtgcccc
    22621 acagcgcgat ctgcaccgtg accaccatct cctccaggta ataagaccac tggctgaagt
    22681 tgggcgggaa gaagctggcg gcgtactcgg ccatgttgcc ggaatcgcgc agcaggtcca
    22741 gggggcgcat gtccgcgccc ttccaggaca gggccagcaa ggccagcaat acgccccagg
    22801 ccatcaggcc cagcaggctg gcgcgcggca cttgcagcgg cgcaggaggc gcctgcacgg
    22861 aaagatggac aggggtgttc acgggcgacc tcagttccgg ctcagctcgg cgagcttgcg
    22921 gtcaatctcg gccaccttgg cctgcttgtc ggcggcgggc aagcttgcat cggcctcgaa
    22981 tttgttgcgc tccttgaaca gctccagctg gcggatgggg atcagttggg cattggtgga
    23041 ggctttgaag gccgacagct tggagatttt catcaggaca tccttttcct gctggccacc
    23101 tttgccgtaa ccatagaaaa agtccttgag cttctgcttg gccgcatcag gcaggtcctt
    23161 gcgcatgacg agcgggtcca gcgggatcag gggggaggtc cagacgacac ggatgtcctt
    23221 gaacttctcg ggcaggcggt ccttgatctt gtccaggttc tcgctgttgt tggtggccac
    23281 atcgacctgt ttgttggcca ctgccagcgc gttgctctca tggttggcac cccgggacac
    23341 cttgaaagcg gttttggcat cgatcttgtt ctgcgcaaaa acgtagtagc tgggcaccag
    23401 aaagccggag gtcgaattgg gatcaccatt gccgaacgac aggttcttgg cattcttgat
    23461 cacgtcgtcc agcgacttga gcgtgctgtc cttatggacg atcaggtgcg agtaatagcc
    23521 ctgggttccg tcggcgttga ccatctgggc aaacacctcg ccgccggcgc ggtccaccgc
    23581 ttccatggcc gacttgttgc cgaaccacgc cacatgcacc ttgccaaagc gcatgccttc
    23641 gatgatgccg gcatagtcag gcgcgaagaa ggcattgacc ttgtagccgg tctttttcgc
    23701 catgtcttcc agcaacggct gccagtccga cttcaggttc tgcgacgact cggtcgaaat
    23761 gatgccgaaa ttgacttcct tgatgtcctg ggcaagggcc ggagcggcca ccagaatggc
    23821 ggcggccagg ccgcctatga atttcctgag catgatgatt ccttatgtga agtgaagtga
    23881 agtgaagaga aaagggtttg tgcttgccgg cggcgcaatg tccgctgtcg gtgacgcggg
    23941 gtctggcatc acgaggtgcg ctgcaattcg gccagcttcc tgttgatcag ctcgagcctt
    24001 gcggccttgt cctgcgcatt catcgccgcg tcggcctcca gcttcgtctt ctcgcgaaac
    24061 agctccagct gacgcgtggg catcagttgg gcatcgcctg attcgcggaa accgctgagc
    24121 gtcagcttgt tcaggatcgc ggtctcgcgc gccgcgtccg gccccttgcc gtagctgagc
    24181 agaaactgtt tgacacgctg cttggtctcc ccgtccaggt ccttgcgcca catgagcggg
    24241 tcgccggcga tcagcggtga cttccagatc acgcgcagct tggccgcgac ctcgggaacg
    24301 cgcgcctgca tgcgctcaag cacatcggag ctgtgcacag cggcatcgac ctgccggttg
    24361 gccaccgcca tgatgttggc ctcgtggttg gcgccgcgga tgttcttgaa ggccgttttg
    24421 ggatccacct tgttcagcgc aaagatgtaa taactcggca ccagaaagcc ggaggtggaa
    24481 ttgggatcgc ccatgccgaa gttcagggac tttccattcc tgagaacttc ctcgatcgac
    24541 tggtacggac tgtccctgtg aacggacacc atggagtgat aaccgatgga cccgtcgctg
    24601 ttgaccacct gagcaaacac ctcggccccg gcgcggtcca ccgcttcgat gcccgccttg
    24661 ttcccaagcc aggcaacctg gaccttgttg aaacgcatgg cttcgatcac accggcgtaa
    24721 tcgggggcaa aaaaggcgtt gaccttgatg cccgtgcgct tttccatgtc ggccagtagg
    24781 ggctgccagt cctgcttcag gttctggctc gactcggtgg acaggatgcc gaaactgaac
    24841 tccttcatgc cctgggcctg caccggcgcc agaatggcga gcgccgtcag aaccagtcct
    24901 gtcaacatgt ttttaatcat ggtttttcct tgcgaataca gggggaacga cggaacccgg
    24961 ctcaggcggc ctgcgccatc ggcgtcggcc acgcgaggtg gggaacgggt ttggccttgt
    25021 cttcgaggct ggaaatggcg tcgcccaggg acaggatgtc gtcggcctcg gcgccataga
    25081 ggtcgcgcag cacggcgggc gtcagtccga ccgacgggcc gtcgtacacc accttgccct
    25141 ggtgcagggc gatggtgcgg gcgcaatact tcatcgccac gttgacctgg tgcagcgaca
    25201 ccaccaccgt acactggtct tcgcggttca cgcgggccag gatgtccatg accttgcgcg
    25261 aggattccgg gtccagcgag gcaatcggct cgtcggccag caccaccttg gcgccctgca
    25321 ccatggcccg tgcaatcgcc gctcgctgct gctggccgcc cgacagcgtc gatgcccgtt
    25381 gtgcactgca ttcggcgatg cccacccggc gcagcgcctc gatacctctg gccttttcct
    25441 cgggcgtgaa ccagcggatc agactgcgcc acatcggtac ctggtgcaag gtgccggcca
    25501 gcacattgac aatcaccggc agacggccca ccagattgaa ctgctggaaa acaaaaccga
    25561 tgcccgaacg caccgaacgg atgttggacg cgattttccc gttgcgctgc acggtcctgc
    25621 cgtggatcac gatggacccc tgctccgatg catcgccggc catcagcccc gccatgtgcc
    25681 gcagcaaggt ggacttgccg gagccggaag ccccgatcag cgcgaccatt tcgccgggcg
    25741 caatgctcag gtcgatttgt tgcaaggcct tgcggccatt gccaaatgtc ttgctcaggg
    25801 tgttgatccg gatggcagcg tccatggtct ctccaagtgg tgttgccgtt gaatctaggg
    25861 tcgcaatgtg tcggtttcgt gtcagtggcg gctaaaaagc cgcgtttcgg ggagcggagc
    25921 acaataagcc catgccttac ctgcccgcct ttatcgcccc cacccgctac gccgacccgg
    25981 ccgccgcact ggcccaggtt cgcttgattt atgaccagca gattgcgcac ctgcgcgatg
    26041 ccatgcaccg ttatgtagct ggcgaaaccc tgcccggcca tgtgcgggcc tgttacccct
    26101 ttgtgcgggt gcacacggaa accgtggcac gcgcgatcct ggaaacgccc gacatttccc
    26161 agctcagcta tgggtttgtg gccgggccag gccgttttga aaccacgctg acgcggcccg
    26221 acctctacgg caactactac ctggaacagt tccgtctgct gatgcaaaac cattcggtcg
    26281 agctggaggt gggcaccagc acccagccca ttcccgtgca tttctcgttt gccgagcacg
    26341 accacatcga aggcaacctg agcgcagaca ggcgcatgct gatgcgcgac gtgttcgacc
    26401 tgcccgacct ggcggccatg gacgacggca ttgccaacgg cacttacgag ccccggccgg
    26461 gcgagccgca gccgctgtcg ctgttcaccg ccgcccgggt ggactactcc ctccagcgcc
    26521 tgcgtcacta caccgggacc tcgcccgact ggttccagaa ctttgtactg ttcaccaact
    26581 accagttcta cattgacgaa tttgtacggc tgggccatga agaaatggca cgcccggaca
    26641 gcgactacat cgcctttgtc gagcccggca atgtggtcac ccgccgcgcg ggcctgccgg
    26701 ccagcatcgg cgacgagctg ggcatggcgc cgccgcgcct gccgcagatg ccggcctacc
    26761 acctgatgcg cgccgaccgc accggcatca ccatggtcaa catcggcgtc gggccggcca
    26821 atgccaagaa catcaccgac cacattgccg tgctgcggcc ccatgcctgg atcatgctgg
    26881 gccactgtgc agggctgcgc aacagccagc agctcggcga ctatgtactg gcgcacggct
    26941 atgtgcgcga agaccacgtg ctggacgaag agctgccgct gtgggtgccg attccggcgc
    27001 tggccgaaat tcaggtggca ctggaacgcg ctgtgaaaga cgtgacccat ctggaggggc
    27061 cggatctcaa gcgcatcatg cgcaccggca ccgtggcgtc caccgacaac cgcaactggg
    27121 aattgctgcc aggcaaccag ccccagcgcc gcttcagcca gagccgcgcc gtcgcgctgg
    27181 acatggaaag cgccaccatc gccgccaacg ggttcaggtt ccgcgttccc tacggcacgt
    27241 tgctgtgtgt cagcgacaag ccgctgcacg gcgagatcaa gctgcccggc atggccaacc
    27301 acttctaccg cgagcgggtc gaccagcacc tgcgcatcgg catgcgggcg atcgaactgc
    27361 tgcgcgccca gggcagcagc cagctgcaca gtcggaagct gcgcagtttt tcggaagtgg
    27421 ccttccagta atccggaaac gcaccatttc cgtgccgtct ggccggtgca tggaagttct
    27481 gagcccgcga tgggtcatca ggtagccaca gctttacaca acttgacggg gcgcaagcga
    27541 atgcgtcatg cgaagagcaa ataatgtgct ttatgcatat gtctggtttt gtcgccccat
    27601 gaccccctcc ccctccagac tccgcccgat gcgcgccccg ttgctcttgg cactggccgt
    27661 gctgctgtcc ggctgcgcat ccctgtcgcc gcagggcggc gcgctgccgc agacgccgat
    27721 tcctgcggcc tggtctggca gcgccaccgc gccggccgcc gcgacctcgc tggcgcattg
    27781 gtggcaacgc ttcgacgacc ccttgctcgg cagtctggtg acccagtccc tgcaagccaa
    27841 taccacggtg cgctccgcgc aggcggcgct gcagcaggcc agagcactgc gtgatgtcag
    27901 ttcggccaat ctgttgccca aactcaacgc cgcgggctct gcccagcgca gcaagtcagg
    27961 cgacaacgcg gccggcaatc tgttcaaggc gggttttgat gcaagctggg agccggatgt
    28021 gtttggcggc aggcgcagcg ccctgagcgc caccgaagcc gatgcccagg ccgcacaggc
    28081 cagcctggcc gacacacagg tgtccattgc cgccgaggtg gcggtgacct atatcgaact
    28141 gcgcgggctg caggcgcgtc tggccattgc gcgcaacaac ctggccagcc agaacgaaac
    28201 gcagcagatc accgactggc gggtccaggc gggactggcg tcctcgctcg acctggagca
    28261 ggcacgtgcc gccagcgcac aaaccagtgc gcaaattccg gcgctgcaaa ccagtgctgc
    28321 gcaggcgcaa tacagcctgg cggtgctgac cggccgggcg cccggcgcgt tgcaggccct
    28381 gttgaccacc accggccccg tgccgcaggc ccccggcgaa ctcgcactca gcattcccgc
    28441 ggaaaccctg cgccagcgcc cggacgtgcg cgcggccgaa catcgcatca gcgccgccct
    28501 ggcgcgcgta tcgcaggctg acgccgcgcg ttatccgggt tttcagatca gcggctctct
    28561 cggcctgagc gccttgagcc tggcctcact caccaccgcc ggcaccgtgg tgaatgcctt
    28621 gctggccagt atttcggtgc cgctgtttga cggcggcgcg gcccgcgccc aggtgcgcgc
    28681 gcaggaagcc gcgctggaac aaacgcgcgt gagttaccag gcgacggtac tgactgcgct
    28741 gaaagacgtg gagaacgcgc tggtgtcgct gcaaggcaac cgggagcggc tggtccggct
    28801 gcaggcggcg gcggacgcgg cgaccaatgc tgacttgctg gcccgccagc gttacgccag
    28861 tgggctgatc gattttcgct cggtgctgga gacgcagcgt accctgctgt cgacccagga
    28921 cagcgtggaa agcacccgcg ccagcctgag cgctgaccat gtgcgccttt acaaggccct
    28981 gggcggcggc tggacgcccg agccgggaac cgccacgcgt tgacagatat gcagagacgg
    29041 cccgaagaac ctgcctccca tcacaaacac cgaccatgaa cagccccacc ccacctgcat
    29101 ccacgccatc tgccgctgat ctgcagaccc tgctgaacga ggaccgcgcc cgcccctggt
    29161 ggcagcgccc cacgccatgg ctcatcgcgc tagccctcct cgtgcttgca gcgggcctga
    29221 cctactggct ggcgcaaaaa aagaccggcg ccgcgccgca ctatgtgacc gaaaccgtcc
    29281 gcaagggcaa tctcacactc aacgtactgg ccaacggcac gctgcaaccg acacgctcgg
    29341 tgaacatcgg cagcgaactc tcgggcacgg tcaagcgcgt gctggtggac gtgaacgatc
    29401 gcatcaggaa aggccaggtg ctggtcgagc tggacacggc caaactgtcc gaccaggtgc
    29461 tgcgctcgcg cgcttcgctg gcggccatgc aggcccaact ggcccagagc gcagccaccg
    29521 tgaaggaaac ccgggccggt ctgacgcgtt ttgaggaagt ggcgcgtctg tccggcggca
    29581 aggtgccgtc ggcgaccgaa ctcgacagtg cccgcgccag cgtggaccgc gccgtggccg
    29641 ccgaggccag cgcccgcgcc aatgtggccg aggcgcgcgc cgctgcctcc accgacgaaa
    29701 ccaacctcgc caaggcctcg atccgttcac cgattgacgg cgtggttctg acgcgcacgg
    29761 tcgatccggg caacgccgtg gccgcctcgc tgcaggcggt gacgctgttt accgtggccg
    29821 aagatctggc tcaattgcgc ctggaagtga acgtcgacga agctgacgtg ggttcggtca
    29881 aggtcggcca gaaggccagc tttaccgtca gcgcctatcc gtcacgccgt tacccggcca
    29941 ccatcacccg cgtggcgttc ggctccacca agaccgacaa cgtggtgact tacgtgacct
    30001 atctggaggt gaacaacacc gatctgagcc tgcgccccgg catgaccgcg gcagccacca
    30061 tcgtcgccac cgagcgcgtc ggcgtgttgc tggtgcccaa taccgcgctg cgcttcacac
    30121 ccacgcaggt cgacagcccc gccgccgcag
//
LOCUS       DAIDFG010000004        28448 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_4_length_28448_cov_18.021062, whole genome shotgun sequence.
ACCESSION   DAIDFG010000004 DAIDFG010000000
VERSION     DAIDFG010000004.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 28448)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28448)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..28448
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4_length_28448_cov_18.021062"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         5079 a         9437 c         8941 g         4991 t
ORIGIN      
        1 gcatcgagat gagcaccgac cacacgctgg aagaagttgg caagcagttc gacgtgacgc
       61 gtgaacgcat ccgccagatc gaggccaagg cgctgcgcaa actcaagcac ccttcgcgtt
      121 cggacaagct gcgcagcttc atcgacacgc tgtaagcacc cggccccctt gccgcgcaag
      181 cggcacggca gcccaacgcc ccggcccgcc cacagcggtc cggggcgttg ttttttgtgc
      241 atggaacgag ctgcagccct catgggttgg acctggggcg cttcataaaa gatagcaaac
      301 tgtgccgcgc aagcggcctt cgcctgcgcc accctacact tggcgttcac cgaactcgtc
      361 caccaaactg ccatgagcgc tgcacgacat gtctgaatcc accgacttcc tcatcatcgg
      421 cggcggcatc gccgctgcct ccgtcggcta ctggctggca ccgcacggcc gtgtcaccct
      481 gctcgagcgc gagccgcagc cgggctacca ttccaccggc cgctcggctg cgctgttcat
      541 ggaaagttat ggcacgcccc aggtgcgcgc cctcaccatg gccagccggg ccttcctgca
      601 gtcgccgccc gagggttttg ccgaacaccc gctgctgggc ccgcgcggcg ccatgatggt
      661 ggcgacccac ggacaggatg cgctgctcgc cgaacactgg gacatcctgc gcgccatgac
      721 gccgcatgcg cagctgctgg acggcgcagg cgcctgcgcc agggtgcccg cgcttcgccc
      781 cggcaaggtg ctgggcgcgg tgtacgaacc cgaggccgcc gacatggacg tgcatgccat
      841 ccaccagggt tatctgcgcg ggttgcgccg cgcaggcggc gtggtggtgt gcaacgcaga
      901 agtcacggca cttgagcgcg ccagtggcgt ctggcgggcg aaggcgggcg gccaagtgta
      961 cgaagcgccc gtggtcctca atgccgctgg cgcctgggcc gacgtggtcg ccgccctcgc
     1021 cggcctgccc ggcatcggcc tggagccgcg gcggcgctcg gccttcatct ttgcgtcccc
     1081 ggaagacgtg aacacgggcg gctggccgct gaccggcggc gtagccgaag actggtactt
     1141 caagcccgac gccggcatgc tgctgggctc gccggccaat gccgacccgg tggcgccgca
     1201 tgacgtgcag ccggaggaaa tggacatcgc cctagccatc caccgcatcg aggagatgac
     1261 cacgctgtcc atccgccggc ccacgcgcac ctgggccggg ctgcgctcct tcgttgccga
     1321 cggtgacctg gtgggcggct tcgatgtcca cgcgccgggc ttcttttggg tcgcggccca
     1381 gggcggctac ggcatccaga cctcggccgc gatgggcgag acctgcgccg cactcgcgcg
     1441 cggcctgccc gttcccgaac gcatcgccgc cttcggcctc acggccgcca tgctcagccc
     1501 gtcccgactg agcgctgcgg cggcctgacc ctcgctcagt ccagcgcggc cagcgccatc
     1561 atgccggcgg ccgtgttgga aatcgggatg tggtagttgg tgtgcacctt gcgcacgcgg
     1621 cccggcacgg cggcgcgcgt ggccagccac atcagcagct ccacaccctg ggtgccggtt
     1681 ttttcgacga gttcatggat gctgtattgc gcggcccact cgggctcgct gatcaggctg
     1741 tccatgaact tcaggtcaaa ggccttgttg atgaaaccgg cgcgttcgcc gtcgagctgg
     1801 tggctcaggc cgccggtgcc tatcaccacc accttcctgt cgctgtccca cgacgcgatc
     1861 gcctcgccca ccgccttgcc cagggccagg cagcgtttgg cgctgggcag cggaaactgc
     1921 acggtgttga tgcagatcgg caccgtcagc accgggcacg ggccctcggg ccagaacagc
     1981 ttgagcggca gcgtgaaggc gtggtccacc agcatttcct ggcaggtcgt gatgtcgaac
     2041 tccttgtcca ccagttcatt gatgatgtgc caggagagct cgggcacacc ccggaacggc
     2101 ggcagcgtgg gaatgcccca gccttcgtcg gcattgcggt actcgggcgc ggcacccacg
     2161 gcaaaggtcg gcatcttgtc gaggaagaag ttcaggccgt ggtcgttgta gaacaccacc
     2221 gcaatgtcgg gcttgacctc gtcgagccag gcccgcaccg gcagaaagcc gtcaaagaag
     2281 ggtttccagt agggctcctg ctgggcgccc cgggcgatcg caccgccaat ggcgggcacg
     2341 tgggaagtgg tcaggccacc gatgattttt gccatgctgc ttttcctcag gccaacagtg
     2401 tggcgggttg atcgttctgc gcgagcagcc gcgccttgaa ggcctccttg ctcaggccgg
     2461 tctgcgcggc acccaggtcc tgcacgtcca gccccaggat gccggcgaac ttggccaggt
     2521 agtacacgtt gccgcccgcc gccagcaggc ccagcacgtt gcgcgtgcgt atggcttggg
     2581 cctgctgccc gttcaggcca taacgaagca tgtagcccac ctcatcggtc ttgaaggcag
     2641 cacggttgtc cgcgttgttg aacgagaaac acatcttgtt gagcgcatag cctttttggg
     2701 cctgctcgcc gtcaaacagc gtggtgccgg gaatcggctt tggcggggaa tgggacacgg
     2761 aaatctcctc gggttgacct gtatcagtac cccagtattg ccgcccgatt acaaaagata
     2821 aaccgatgaa tgctgatgga acccatgaat gaatccgatg gatccgggct ggacgccagg
     2881 ttgctgcagt tgctgctgac cgtgctcgac accggcagtg tgactgcggc ggcgcagcgc
     2941 ctgggcgtga cgcagtccgc cgtgagccac ggcctggacc ggttgcgcgc catcaccggc
     3001 gacccgctgt tcgtgaaggc cggccgcggc atcgtggcca ccgcacgcgc cgaggccctg
     3061 gccctgccgg cgcgcgaact gctgggcgca ctggagcgtt ttgcgcggct cggcagcttc
     3121 gaccccgcgc gctggcaaac caccttcacg atcgccgcca atgacttcca gcgcgacctg
     3181 ctgctgcctg cgctggccgc gcgcctgcgc atgcaggcac cgggcgtggt gctgcgcgtg
     3241 atcccttccg cagtgccgcg gctggaaaca ctgcgcagcg acgactgcca gctggccatc
     3301 agcccgcgcc cgcccgaagg cagcgacatc gtgcaaaaac gcctgttcga agaccagtac
     3361 cgggtctttt atgacccggc ggaacgcgag gcgccgcgta ccgaggccga ctacctggcc
     3421 gccagccacg ccaccgtggt gtacgagccg cgccgcagcc tggagctgga ccaggtgctg
     3481 gcggcgcgcg gcctgaagcg gcatttcacg gtgatggtgc cgtccttcag cgcactgccg
     3541 gcctttctgc gcggcacgcc gttgctggcc actgtgccgg ggctgctggc gcaccacagc
     3601 ttccagggcc tggccagttg ccagccgcct gtgccttgcc cctccatgcc catgtacgcg
     3661 atctggcacc tgcgctacca gcaggatccg gcgcaccgct ggctgcgcga acagctggat
     3721 gccgtggtgg gcccggcgct ggccgacgcc gcaccgggct agattttcag taaaacaggc
     3781 cgctagccct tatggataaa ccgcagggag ctatcaatac aatagcattc cgccggaatc
     3841 cgatcaagcg gcaaattcct cggtgtccac ttcgctgaca ctctgtgcgc tgtagcggtg
     3901 tccggtgacg gtgtcctggt tgaccagcgc ttccagcctg gccagcaggc ccgcgtccag
     3961 ccggacgttc atcgcgccga ggttgtcgtg cagatgctcg atgctggtgg tgccgggaat
     4021 cggcacgata tgcggtgcct tgtgcagcag ccaggccagc gcgagctggg ccggcgtgca
     4081 gcccgcctcc tgcgccagcg ccttgtaggg cggcagcagc tgaaggttgg ccgcgtagtt
     4141 ctccggcgag aagcgcggca tggccttgcg gatgtccttg gcgtccaacc tgctcacgtc
     4201 gtgcagcgca tcgcagagga agcctctggc aaccggactg aaggcgacaa acgtcgcgcc
     4261 cagctcgcgg caggcctcca gcaccgcgat ctcggggttg cgcgtccaca gcgaatactc
     4321 ggtctgcacg gccgcaatcg ggtgcaccgc atgggccttg cgcagcgtgg cggccgacac
     4381 ttcggacagg ccgatggccc gcaccttgcc cgcgcgcacc agatccgcca tggcacccac
     4441 gctgtcctcg atcggcacct tcttgtccca gcggtgcagg tagtaaaggt cgatcacgtc
     4501 ggtctgcagg cgctgcaggc tgtcttcgca gttcttgcga agggtctcgg gccggccgtc
     4561 gatcacgcgt ttgacgccgt cctcgaactg cacacccgcc atgccgccct tgctggccag
     4621 tgtgaaacgg ctgcggtgct ttgacagcac cttgcccacc agcgtctcgt tggcaccgaa
     4681 gccgtacagc gccgccgtgt cgaaaagcgt gacgccggca tcgagagcgg ccagcagcac
     4741 acgttcgccc tgctccgccg atggcggcgc accgtaggcg tggctcaggt tcatgcagcc
     4801 caggccgatg gctgtaacgt ggaaggggcc tatggttcgt tcttgcatca ctcaagctta
     4861 ctggaaacga agctggatcg tggcgccttc agccgccggc ccgccaatgt cagccaggcg
     4921 ctgccgccat aggcggccac caggtcatgg gcggtctcgg cccatgcggg atggctggcg
     4981 tccatgccca actcctcaat gaaatctgcc tgaacaacca tgaactggcg ccggtacggc
     5041 aacaggaacg cccgcggatc agctttcaga gccgtttgcg cgtcctcgta cctggccagc
     5101 cacagattga gcaacatgcc ggtctgcggc gcgtgccaga acgtccccat gtcatcctcc
     5161 cgcgtcgcca ggcctcccag cacgacccgc gcgtgttccc agtggacaaa gcccacctcg
     5221 cgcgccacct ggttcaggca atgacgcagg cgtagctcgc caggccccgt ctctccccgc
     5281 cgggcggcgt tgagaccaag ccgcgcacgt gtcttcaatt catcgagcag cgcgctcacg
     5341 tgagctccag cacggtgctg cggcaaagct gtgacagcat ggccctcaaa cgctcccgcg
     5401 ccgcatcatc catgcccgcc agcgcttccg agcaaacagc ctcggcggtc actgcagcct
     5461 cggtcacgcg cgcccgtccg gccggccgca gtgccaccat gcgcccgggc tcccgctgga
     5521 gatagccggt cttttcgagg ggcgcaagct gccggaccac ggccgacatg cgtaccccca
     5581 ggggtcgcac caggccggcc agcgggaggc gcccgccttc tgcctgtgcc aggaggcgca
     5641 gcagaatgaa ctcgcccagg ctcaggccat ggaagaggcc cagaacgtcg tccagtttca
     5701 gcgtgaggct tgcatgcgcc aggctgacct gcaggcaatc gtcaagtgca ttggctttca
     5761 tacacccagt tcctcgcgcc acatcgccag cagcccggcc agctccgcct tgtagtcgaa
     5821 ggccgggtcg tctcgtcgct tgacgaggtc gatgacttcg aagcggcagt tgtccggccc
     5881 gaaggccagc cagtcctgtt gcaggtgttt gtcgcggtag gacttgcggt ccagttcgaa
     5941 gcgggcgcgg ttcatcgccg cgtgcacgtt ggtgctggct tccacaatca cgcggccgct
     6001 ggacaggctg cggatggcgt agacgcccat gggcggcggg gtttcccggt actgcttttt
     6061 cagctcgctg cgcgtgcggc cggcagaagc cggcgcggga acagggggaa aagaaagggt
     6121 tgatgccatg acaagactcc tgggtgcgct acatgccaat cccgcgcttg gccagcgaac
     6181 ccgtgatctg tcaggttgat aaaaaatgaa ggagggtgag agcctcttgt gcgggcaggc
     6241 gcccctcagc gcctgagccg caattataaa ggcccgaggg cctgttaaca ggccctagtt
     6301 cagttgctgc tccaggtcgt gcagcacctg gtaacacggc agcactttgg cgacgctgga
     6361 attgggctcg cggcccgcgc ggatggcggc gaaaaattcg cggtcctgca gctcgatgcc
     6421 gttcatggac acgtccacct tgctcacgtc gattttttct tccttgccgt tgaacaggtc
     6481 gtcgtaacgc gcaatgtagg tggcgctgtc gccgatgtag cggaagaagg tgcccagcgg
     6541 cccgtcgttg ttgaaggaca ggctcaaggt gcagatcgcg ccgttggcgg ccttgagctg
     6601 gatgctcatg tccatcgcaa tgcccagcgc cgggtgaatc ggcccctgga tggcgttagc
     6661 cttcacgatg gggctgccgc actggtaggc gaacaggtcc accgtgtggg ccgcgtggtg
     6721 ccacagcagg tggtcggtcc agctgcgcgg ctggcccagc gcgttcatgt tgctgcggcg
     6781 gaagaaatag gtctgcacgt ccatctgctg gatgttgaag cggcccgccg caatttcctt
     6841 gtgcacgtac tggtggctgg ggttgaagcg gcgggtgtgg ccgcacatgg cgaccagccc
     6901 ggtctgcttc tgcagggcca ccacctcctg ggcgcccttc agggtgtcgg ccagcgggat
     6961 ctccacctgc acatgtttgc ccgccttcat gcaggcaatg ctttgctcgg catgcatctg
     7021 cgtcggcgtg cacaggatca cggcgtccac ctccttgagc gccaggctgt cggccaggtc
     7081 ggtggtgaca tggccgatgc cgtatttggc agcgacttct ttcgtcttct ccagatcgcg
     7141 gctgatcagc gagatgactt ccacgccatc gatgttcttg atgccgtcga ggtgtttgat
     7201 gccgaaggcg cctgcaccag ccaatgcgac tttgatggtt ttgctcattt acttgttctc
     7261 cagaatcagg tggcccaccg ccgtattgct ggcgggcaca tggtaaaagc ggtgggcgac
     7321 cttgggcgcg gggccaccgg ccacgtcgga catggcgccg cgggcgatta gccacatcac
     7381 gagttcgatg ccctccgagc cggcttcgcg cacatagtcg atgtgcggct tgccggccag
     7441 gcctgccggg tcggtgatca gctggtcgag gaaggcgttg tcgaactcct tgttgatcag
     7501 gccggcacgc gggccctgca gctggtggct catgccgcct gtgccccaga tctgcacctt
     7561 cagcggcttg tcaaaactct ccactgcctt tcggatggcc ttgcccagct cgaagcaacg
     7621 ccggcccgac ggcacggggt actgcaccac gttgacggca aacggaatca ccgggcaggg
     7681 ccaggcttgc ggctggccgc acatcagcga cagcggcacg gtcaggccgt ggtccacgtc
     7741 cattttgttg acgatggtga ggtcgaagtc ctgctggatc accgactgcg caatgtgcgc
     7801 ggccagttcg gggtggccga tgaccttggg cacagggcgc ggaccccagc cctcgtcggc
     7861 cggcgtgaac tcggccgcgg tgccgatggc gaaggtcgga atcatctcca ggctgaacgc
     7921 cgtggcgtgg tcgttgtaga ccaggaaaat cacgtcgggc gggttgtcct tgagccactg
     7981 tttggagtag tcgtaaccct tgaacacggg ttgccagtaa ggctcgccgc ttttgcccag
     8041 gtcgagggcc gcgccgatgg cgggcacgtg cgaggtgtaa acggatgcgg tgattttggc
     8101 catgttcagg cactcgcttt ctggccggct ttgccttggg gttggtgctg ggcctgcgca
     8161 gtgccgtctt cgccgaccac gcggtttccc tcgacggagc ggccgccacc gatcatcatg
     8221 tcgcggtact cctcttcggt catgccggtc atgctgcctg ccatctgctg aaagctcttg
     8281 ccgtcggtcg cgccgatttt ggccaggaag tagatgttgc cgccggcctt gatgcagcgg
     8341 ttcaggtcgc gcgccagcac cgcctgcttc tgctcttcgg tcatgggcca ctcatcgagg
     8401 taggcgcgct cattggcctt gaagcgttcg cggttggcgg ccttcatcag cgacatgcag
     8461 aactggttca ggtggtagcc caggcgggat tgctcggcgt caaaaatcgt ggtgccgggg
     8521 acatcgaggt aaggtttttc gagtgacatg atggtttctc tttgaccagt ggttgaacct
     8581 gacgcagtca gctccagtaa agccgggtcg ggttgtcgac gagcagcttg cgctgcaatt
     8641 cgggcgtggt ggcgatatgg ggaatgtagt ccaccagcag gccatcgtcg ggcatgtggt
     8701 ttttcaggtt ggggtgcggc cagtcggtgc cccagagcac acggtcggga aaggtctcga
     8761 ccaggcgccg ggcaaaaggc accacgtcgc ggtaggcgtt ctgctcgccg ttcagcgccg
     8821 gcgggccgct cacgctgagg cgctcgggac agctgacctt gctccagatg ttgtcgtgct
     8881 cgcgcatcat cttgacgaac agctcgaact cggggccgtc caccggcttg ctcacgtcgg
     8941 gccggcccat gtggtccacc accaccgtgg tcggcagcgc ggtgaaaaag tcgtacagct
     9001 cgggcaggtc ctgtgcctcg aaataaacca ccacatgcca gcccaggggc gcaatgcggt
     9061 tggcaatctc cagcaacacc tcgcgcggcg tgaagtcggc caggcgccgg acaaagttga
     9121 aacgcgtgcc gcgcacgccg gcttcatgca tctcctggag ctcggcatcg gtgacgttgc
     9181 ggttgacggt agcgacaccg cgcgccttgt tgttcgagtg ccgcagcgca tcgaccagcg
     9241 cacggttgtc gctgccgtgg caggtggcct gcacgatcac gtttttatcg aaacccaggt
     9301 ggtcgcgcag cgcgaacagc tggtctttgg aggcgtcgca cggcgtgtac ttgcgctcgg
     9361 gcgcataggg gaactgcgcg cccgggccga acacatggca atgcgcgtcc accgcgccgg
     9421 cgggcagctt gaagcggggt ttggcggggc cgtcgtacca gtcgagccag cctggcgttt
     9481 tggtgaaatc gcccgtcgca gttttttcca tggtcagcat tcagtcggtt tttcagtcag
     9541 tgtgtcagtc gatgtacttg aggccggcct tggccagcgg ctcccgcatg ttgtacatgt
     9601 ccaggcccag aatgccagag gccagtttgg cgcgtttttc gccttcgttg gcttcgcggg
     9661 cttcggcggc gtcggccacc ttcttgaccc actcggccgg caccaccacc acgccgtcgt
     9721 cgtcggccac caccgcatcg cccgggttga cactcacgcc cgcgcacacc accgggatgt
     9781 tcaccgaacc cagcgtggcc ttgatcgtgc ccttggcgct gatgcccttg ctccacaccg
     9841 ggaagccgat ttcctcgagc gtcttgacgt cacgcacacc ggcgtcgatc accagcgccc
     9901 gcgcaccgcg cgccatgaag gacgtggcca gcaggtcgcc gaagaagccg tcacagttgt
     9961 cggcggtgag ggccgccacc acgatgtcgc cgggctggat ctgttcggcc accacatgca
    10021 tcatccagtt gtcgccgggg tgcagcagca cggtcaccgc ggtgcccgac acctgcgcgc
    10081 cgcggtagat ggggcgcatg tagggcttca tcagcccgac gcggcccatg gcctcgtgca
    10141 cggtggcggc gcccaggcgg gtcagccggt cgacggcggc ctggtcggcg cgcacgatgt
    10201 tgcgtttgac aatgcccaga gtggtcatga ttcgtatctc cgtcaattaa aggcttttgg
    10261 ccttgaggcg cgcatcgagg cgcggaaaca cacggcgcac attggcctcg tagatctggt
    10321 ggcggtcatc ggcgctgagg atcttgctgg cttcgatgta gcgcttggtg tcgtcgtagt
    10381 agttgccggt ttcggggtcg atgccgcgca ccgcgccaat catctcggag gcaaacaaca
    10441 cgtttttcac cgggatcacc gtgttcagca ggtcaatgcc cggctggtgg tagacgcagg
    10501 tgtcgaagta aatgttgttg agcaggtgct cggacagcag cggctttttc agctcctgcg
    10561 ccaggccgcg gaagcgcccc cagtggtagg gcacggcgcc gccgccgtgt ggaatcagga
    10621 agcgcaaggt cggaaagtcc ttgaacaggt ccgaggtcag gcactgcatg aaggccgtgg
    10681 tgtcggcgtt caggtagtgc gcaccggtgg tgtgaaaaca cgcgttgcag gaggtcgaga
    10741 cgtggatcat cgcggggatg tcgtactcca ccatcttctc gtagatcggg taccagtgtc
    10801 tgtcggacag cggcggcgag gtccagtggc cgcccgaagg atcgggattc aggttgatgc
    10861 cgacgttgcc gtagtccttc acgcactttt ccagctcggg gatgcaggtg gccggatcga
    10921 cgccgggcga ctgcggcagc atcgccaccg gcacgaagtt gtcgggaaac agctggctga
    10981 cgcggtagca gagttcgttg cagatggccg cccaggtgga cgagacattg aagtcaccga
    11041 tgtgatgagc catgaagctg gcacgcggcg aaaacagcgt gatgtcgctg ccgcgctctt
    11101 tcatcaggcg cagctggttg gtctcgatcg actcgcgcag ctcgtcgtcg ctgatcttca
    11161 gctcagccac cctgggcatc atggccgggt ccttgatgcc ggcgatctgc cggttgcgcc
    11221 actcttccag cgccttgggg gccgtggtgt agtggccgtg gcagtcgatg atcaatgatt
    11281 ttttcataca ggtctcctgg aaatgcaagg gggtgtcggt caggcggcgg ggtccatgcc
    11341 ctggcggcgc ttggcctcgg cggcctgcgg cgtgttcatg gatgcgagca tggcgcgcac
    11401 cgcttctgcg ttgcgggtgc cggtgcacac gccggcagaa aaagtggtga tgatctggat
    11461 ggccggcggc aaggggccca gcacggtgat gccctgcagg tgcatcagtt cgctcagttg
    11521 ctgaaaaccc agctcgacct cgccgcacgc caccagcgaa ccgaccggaa tgcccggcgg
    11581 cggagtcacg atgcggttct ggatctcgcc ggcgatgccc cagcgctcga acaagccggc
    11641 cagcgccacg ccgctgggcc cggtcgagta gctgatgctg cgcgcggcgc gcaccgcctc
    11701 gcgcaatgcg tcttccgtcg agatgtcggg cctgggcgcg ccggcgcgca cggcaacggc
    11761 cacacccgag cggaccaggt ccaccctgct gccggccagc acatggccgg aagcgatcag
    11821 cctgtcgatc gcgtcggatg ccaggatcac gacatcaaag gcctcgccgg cctgcacgcg
    11881 cctggccgca tcgacgccgc cgaccgattc gatggccacg cggcagtccg attgctgctc
    11941 gaaagccgtc accaggtcgg ccagcaccag gcgggtggcc atggaggaaa tgcccttgag
    12001 gtcggtcgtc atggcagtcg gtcagcgtgg agggtcatgc gattgattgt gacgacggcc
    12061 ctgcctcccg ccaaggcagc gcggtttcta gctgatataa cataccggca ttgatcgacg
    12121 cctgttatat ccattcagga caactacagg acaacccgcc acccttcccc gtcatggacc
    12181 tcaagcagct tgaatatttc gtacgcgtgg ccgaactcgg cagcttcacg cgcgcttcca
    12241 ttgcgctgga cattgcccag ccggcgctga gccgccagat ccgcctgctg gaggtggagc
    12301 tgcggcagaa cctgctgacc cgcaacgggc gcggcgccct cccgacggaa gcgggcaagc
    12361 tgctgctcaa gcacggacgg ggcatcctgc accaggtaga ggtggcccgc gaagaactgg
    12421 gtgctgcgcg cggcgccctg gccgggcgcg tgtccatcgg cctgccgccc agcctgtcca
    12481 aactcatcac ggtgccgctc acccgggctt tccgccagca gctgccgcac gcccagctga
    12541 cgctgaccga aggcttttcc gtgctgatgc acgagggcct gcgtgtgggc aacctcgaca
    12601 tggccgtgct ctacaactcc gagcgttcac ccgagctgga aatcaccacg ctgcactccg
    12661 aagagctggt gctgatttcc cccaagacca gcgtcaaggc cggtgccgcg aaaaaaggcc
    12721 gggccggcaa gcggcagccc atcagcctcg ccgaggtggc cgagctgccg ctgatcctgc
    12781 ccagccgccc caatgccttc cgcatcctga tcgagagcga aatgatcacg ataggtcgca
    12841 agccgcagat cacgctggag gtggacggcc tgaacgccat cctgagcctg gtcaaggaag
    12901 gcatgggaca tgccgtgttg ccaagctaca cgctgagcaa cgtcgatgat cccgaaccct
    12961 tcctgctgcg cagcatccac tcgccgcgca tcatgagcga gctgatgctc gcgcgctcct
    13021 cacgccgcgc cagtaccgac acgcagaaaa tggcggtcga ggtggtcaag tcggtggtgg
    13081 tcgaggccat caagccttat gcctgacccg gttactgcaa cagccggtcc gcgtagtcct
    13141 gccagcgggc gtccttgccc agaccggcaa acacaccggc ccgggcctgg tcggccaccc
    13201 actgctgctt gtcggcaatc gccgacgcca tgaaaggctc gaagccgccg ccggcctcgc
    13261 gcacggtgtt ggccgcctcg cgcatttcct cggcacggcg ctggccgtgc tgcaccaccc
    13321 ggctgaaaaa gtaggcgccc tgcttctccc agtcgatgct ggggaaggtt tcggccagcg
    13381 tcggcaacac gtggtcctcc acgccgtagg cacgggccgt ggtgtaactc tcgatcacca
    13441 gcgcctccag gcccttgatc atcacgctgc ggcacatctt gatggcgctg gccacgccca
    13501 gcttttcgct gaccgccttc gcatccatgc cccaggccat gaacacgccg gccagttcag
    13561 ccgcacgcgt gccgcccagc agcatgggca cctggatgcc ataaggcggc accgaggtca
    13621 tcacaccggc ttcgacatag tgcccacccg ccgcatcaat cagggccgcg gcctgctgct
    13681 tggtaccggg tgaggcggaa ttgaggtcca gaaaaaccgt gccgcgccgg acatgggcgg
    13741 cggcggcctg cgcgaccgcc agcgtgttgg acgccgtgac cgcagagatg atcaggtcag
    13801 cccgcccgca cagcgccgcc atggacgcgc acgccgccac accggcctgc gccgcatggg
    13861 cacgctgcgc ctcgcgcagg gccggtgcct cgaaggtgag gtcccaggcg ctgatgcccg
    13921 ccaccctgtc cttgaggccg gcggtgaaaa tttttccgac ctcgccgtag ccgatcatgc
    13981 cgatatgttc aaacttcacg gatgtttctc ctgctttttc tgcttcacct gcggccgacc
    14041 ggtcgcttca tgaacgtcat tactgagtcg gaatcctggc gcgccggatc acctcgcccc
    14101 agcgcctggt ttcaccggcc agcagttcgg cggcctgttc gggcgaactg ccgcgcgctt
    14161 cgacgttgag ctcggccagc ttcctgcgga cctccggcgc gttgacggcg gccaccactt
    14221 cgcggttcag ccgggcgatc acggcgggcg gcgtcctggc cggcgcggcc agcgcgttcc
    14281 agctggaggc cacaaaggtg ggcaggccgc tttccctggc cgtcggcacc tcgggcagca
    14341 ccgcggcacg cctgtcgccg gtgacagcca gcgcacgcag ggcaccggcc cggatctgcg
    14401 gcaggatggg gccgaggatt tccacggcag catcgatctg gccgccaagc agggccgtga
    14461 ccaccgccgg cgtgccgttg aacggaacaa tctgcacgtc ggcgccggag ctgatcttga
    14521 acaattcggc cgcgaggttc tgcgtgctgc cgatgttgat gcttccaagg ttgagtttgc
    14581 cggggttggc cttggcaaaa gccagcagct cgcccagggt cctgaatttc gaatcggcgg
    14641 gggtgaccac ggcgatgtca aaaaagccca gcgtggagac cggcgcgaag tcgcgcaccg
    14701 catcaaacgg cagcgacttg aacaaacccg cactgaccgc cgtgccattg gacatcagca
    14761 gcagggtgtg gccgtcgggc tgcgccctcg ccaccatctc gccggccacc acgccgccgg
    14821 caccgggctt gttgtcaacc agcaccggct ggcccagcga ctcgctgagt ttttgcgcca
    14881 ccgtgcgcgc ggtcaggtcg gccacgccgc cggccccgaa aggcaccacg atccggacgg
    14941 gtttgctcgg gaaggtttgt gccgcggcca gcgaagccag cagcagggag gccagaagac
    15001 cggtggcaag gcgttgaaaa gtcatgtggg tgtcccgtgt gtcgtatcgc tggtaacggg
    15061 gcattttggc gtataccgcg ctgcaccgaa agcggccccg ccttctggca gccatcacaa
    15121 acaggcctga agggtgcagc cggctttgta aaaataatga gcccaaagtg gccccggccc
    15181 atcacagccg ggcgcaggca gctattgatt caatagcacc gcgagggcca tgcgtgtcgc
    15241 gcttacagcc tcaacgcaaa cagcgtgatg gacggaaagg ccgccaggat cacgatgcgc
    15301 accatgtcgc tggccacaaa cggcatggcc cccttgaagg tctggctcat gggtatgtcc
    15361 cgtgcgatcg agttgatcac aaacaggttc atgcctaccg gcggcgtgat catgcccacc
    15421 tccaccgcga tcagcaccag aatgccgaac cagatcgcaa actcttcagg ttgcaggccg
    15481 tagtcgagcg ccgtgacgat gggaaagaag atcgggatgg tcagcaggat catggacagc
    15541 gagtccatga agcagcccat cacgaggtac aacagaagaa tggccgccat gaccatccat
    15601 gggctcatgc ccgaggcagc caccatcgag gcggcctcct gcggcaactg cgagagtgcc
    15661 aggaaggtgt tgtacacgcc ggcgcccagc acaatcatga agatcatgcc ggtggccatg
    15721 gcggtgccca ccagcgcttc gcgcaattgc ttgcgcccca gcgagccgtt gaccaatgcc
    15781 atcaggccgg tgctggcggc ccctatggcc gcgccctcgg taggtgtgaa aaggccgccg
    15841 taaattcccc caaccaccac gatgaagatg gtcagcaccg gccagacctt gcgcgtggca
    15901 cgccagcgct cgggcatagg catgggcgcc agcttgccgg cgctgccggg gctgacgcgc
    15961 acataaaggg ccaccacgat catgtagctg atcatggcca ggatgcccgg aacaaacgcg
    16021 gcaaggaaca gcttggcgat gttttgctcc gtcaggatgg cgtagatcac cagcacgatg
    16081 gaaggcggaa tcaggatgcc cagcgtaccg cccgcagcca ggcagccggt cgccagtgcg
    16141 cccgagtagc cgctcttgcg cagttcgggc aaggccacct ggcccatggt ggcagccgtg
    16201 gctaccgacg aaccgcagat ggcgccaaaa ccggcgcagg cgccgatggc ggccagcgcc
    16261 acgccgccgc ggcggtggcc cagccaagcc tcggccgcct tgaacagcga cgccgacatg
    16321 ccgccaatcg aggcgaactg ccccatcagc aaaaacagcg gaatgaccga cagggagtag
    16381 cttgagaagg tgacataggt cacctgcttg agctggttga gaatcggcgc gatgctgcca
    16441 taaaccaccc atgccccggc aaaaccacac agcagcatgg acagcccgat gggcacgcgc
    16501 aggaaaatca gcaccagcag gacagggaac gaccacagcg cgagttcaat accgctcatg
    16561 cccggccccc tgcacccgcc acatcgtgta ccacggcgct gctcatacgt gtccccctgc
    16621 gggtccggcc gaccagccct cgggcgggcg ggccacaccc atttccacca gcgtcttggc
    16681 caggtaggtc aggcaaccca tcacggcgcc gaacatgcac agcaccaatg cccaccacac
    16741 cggtatttgc aggatgaagg tggtctcgcc ttccccatac ttgtcaaaca atgagacgcc
    16801 gaggcgccag gtcagtacgg tccaggtcaa gagcatcaac aagtcgcttc ctatggtgac
    16861 ggcgcgctgc gcccagccag gcaggtgcat gtacaccagg tccaccatgg cgtgcccgtt
    16921 tttcaggtaa caccagggca ggaaaaagaa aacaaccacc gcggtggcca tttcaaccat
    16981 ttcaaagtcg ccgggaacag ggcccagccc gacattgatg agcgcgcggc ccgtgacgct
    17041 gacaaccacc atcagcgcca gcgccgacag aagcagcccg ccgatgtagg ccagccactc
    17101 agccgcgcgg taaacaaatt tgaccatgtt gtatgtggcg cggggcacct aggtgccccg
    17161 ctgcatgaag ttgaatgtcg ggaatctctt atttggagta tttgtccacc agcgcccggg
    17221 cttcggcggc cagcttggtg ccgtcaatgc ccttgccttt catttcggca atccagtccg
    17281 actcgaccac actcgccgtg cggcgccagc gctgcgtctc gaccacgtcc agcgcgacga
    17341 tcttgttgcc cgccttgacg gcaatctcgc ggcccgccac gtcgccggcg tccatggccc
    17401 ggcccagcat ggccgcagcc tcgatgccgg agttggcgtc gatcaccttc ttcaggtcag
    17461 ccggcagttt ttcgtaggaa cccttgttca tggcaaacac aaaggtctgc gtgtacaggc
    17521 cgtatttgcc ggcgaaggtg gtgtggttct tcacgagctg ggacaccttc agtgccggcg
    17581 tcacttccca gggaatggtg gtgccgttga tcacgccctt ggacagcgct tccgtcacgg
    17641 ccggcacagg catgcccacc gggatggcgc cgagcttggt caacatgttg ttgatgacgc
    17701 gcgaaccgcc gcgaatcttc atgccgcgca gcgactccag gccggtgacc ggctccttgg
    17761 tgtggaacag gccggggccg tgggtatgca cggcaatgac cttgacgtcc ttgaactcgt
    17821 ccgcggcgaa tttttccatg tactcctgga acgcacgcga accggcttct gccgtggtgg
    17881 tcatgaacgg cagttcgaac acttcagact tcgggaaacg gccaggcgtg tagcccagca
    17941 cggtccaggt caggtccacg ataccgtccc gggcctggtc aaacaattga gggggcgcgc
    18001 cgcccagctg catggcgtga aacagctgca ccttgatctt gccgccggat tccttctcca
    18061 ccttctgcgc ccagggaatc agcgccttgg ccggaatggt tgcctgcggc ggcagcatct
    18121 ggtgcaggcg aagagtgacc tgttgtgcaa aaccggcgcc ggcagcgcac agggcagcaa
    18181 ccgccacggc ggttttgaaa agggctcttt tgttcatttc agtctccggt aatagtgttg
    18241 aaaacagagg gtactgctgc gttggcaggt cttccatggg gtcattgcgg attgtcgccc
    18301 cgcaaaaccg ccaatggaac ggcggacagg gctctatcag ttatatgctt ttgatatatc
    18361 aaatatccgg atatctcaat acccgtccgc gccataccgt tccttcaccg accgtcatga
    18421 aggaccgctc agcttgttga ccaaggttgt gatcagtcgc ggcggcgaat gagtttgatc
    18481 gcccgcatcg attcaggcat ctgcgagccg gtctggcccg acagcgcgtg gagcaggttg
    18541 cgctgggcga tgcgcgagtg ctcgcgcatg atggcttcgg cgcgcgcgcc ttcgcgccgc
    18601 tcgatggcgt ccagcacctg gcggtgctgg tcctgcgcca cgatcagcat gtcacgcgcc
    18661 tgcggcgagt tggcctgcgc cacgacaaac cccgagggtg aggcaaacgg caggttcacg
    18721 acgcgttcga gttcacgcgc aatcaccgtg ctgccggcca tctcgctgag caggttgtga
    18781 aagcgctggt tcagcgtgac gtaatgcagg aaagactcgt cgtcgagggc cggctggctc
    18841 agcacgccgt cgatctggcc gagacaggcg cgtgcctcgg ccagcagcgc gccagacacc
    18901 ccgcgttcgg ccgccagccg ggctgacagc ccttcgagcg tgccgcgcag ttcaatcgcc
    18961 tcggacacat cgcgctccga aaacgtgcgc accgcataac cgccattgac cagcccctcc
    19021 agcaggccct cctgctccag cctcatcagc gcggcgcgaa tcggcgtgcg cgacaccccg
    19081 agcttttcaa caatggccag ctcggcaata cgcgcgccgc cgggcagctc gccggccaga
    19141 atcatttcgc gcaggcgcag ctgcgccttg acggtctggg aagcgcccgg ctcctgcacg
    19201 tcggcggcgg ccagggaagt ggttttgtcg ttcatctgga tctcttaatg tatacagcaa
    19261 tgacctgttc agattgaatt ttgccctatt cagcgtatat tttgataaat taatgatatc
    19321 aatgtataca tcaatctcgt ttttttgcat aatcgccgcc tgagccatca gccgtccaaa
    19381 tcggcctggg ccacccaaaa aacctccccc acacccaccc tccccctcct ctacggacaa
    19441 gcgtcatgtt tcccaaaaac acctggtatg ttgcctgcac tcccaccgaa atcgacgaca
    19501 agccgctggg ccggcagatc tgcggcgaaa aaattgtttt ctaccgcggc cccgaaggca
    19561 aggtcgttgc gatggacgac ttctgcccgc accgcggcgc ggccctgtcg ctgggcacgg
    19621 tctgcgaagg caagctggtc tgcggctacc acggcctggt gatggaatgc gacggcaagg
    19681 tcgcctccat gcccggccag cgcgtgcagg gtttcccgcg caaccgcagc ttcccggtgg
    19741 tggagcgcca cggcttcatc tgggtctgga ccggcgacga aaaactggcc gacgacagca
    19801 agattcccca gctcgactgg gccgacaacc ccgagtgggc ctatggcggc ggcctgtacc
    19861 acatcaactg cgactaccgc ctgatgatcg acaacctgat ggatctgacg cacgagacct
    19921 acgtccacac caccagcatc ggccagaagg aaatcgacga aacgcccgtc agcaccaaaa
    19981 ccgagggcga gagcgtggtg accagccgct tcatggaaaa cgtgatggca ccgcctttct
    20041 ggcgcgccaa cctgcgcggc aacaacctgg ccgacgacgt gccggtggac cgctggcagg
    20101 tctgccgctt cacgccgccc agccacgtcc tgatcgaggt cggcgtggcc cacgccggca
    20161 agggcggcta caacgctgac ccgaaagaca aggtcagcag catcgtggtg gacttcatca
    20221 cgcccgaaac cgaaacctcg atgtggtact tctggggcat ggcacgcaac ttcaacccga
    20281 aagacaaggc cctcaccgcg cagatccgcg agggccaggg caaggtgttt gccgaagaca
    20341 ccgccgtgct ggaagcgcag cagcgcaacc ttacgctgtt cccgcagcgc aagctgctga
    20401 tgctcaacat cgacgccggc ggcgtgcagt cgcgccgcat catcgaccgc ctcatcgcgc
    20461 aggagcaggc acccgcgcaa gccccagctc aacagcaggc cgcctgacca tggacagtct
    20521 tgacgtcaag gtcatccgaa aaaccatgga agccgaaggc atcaccagct tcgagctggc
    20581 ccgcgccgac ggtgcgccgc tgccggcctt cagcgcgggc tcccatgtcg atgtgcagat
    20641 tcccggcggg ctgacgcgcc agtactcgct gtgcaacgac gccaccgagc agcaccgcta
    20701 ccgcatcgcc gtgctgcgcg acccggcctc gcgcggcggc tccgtcgcca tgcacgaggc
    20761 ggtgaacgaa ggcgacgtga tccgcatcag cccaccgaaa aaccactttc cgctgcagca
    20821 cgcgccgcgc acactgctgc tggccggtgg catcggcgtg acgccgctgc tgtgcatggc
    20881 gcagcggctg gcggccatcc atgcggactt caccctgcac tactgcaccc gctcggccga
    20941 acgcaccgct ttccgccagg agatcggcga ctcggccttt gccggcaagg tgtatttcca
    21001 ctacgacgcc ggcccggccg agcagaaact cgacctgcca gccgtgctgg ccggtgccac
    21061 ccccgagcac cagctgtatg tctgcggccc tgccggtttc atcacccacg tggtggacag
    21121 cgccaaggcc ggcggctggc gcagcgagca aatccacctc gagtatttcg gcgccgcgcc
    21181 gcaggacacc gctggcgaca ccggcttcga ggtgaagatc gccagcaccg gcaaaagcta
    21241 caccgtgggc gcggagcaga acatcatcca ggtgctgaag gacaacggca tcgagatcct
    21301 cacctcctgc gagcagggcg tctgcggcac ctgcatcacg cgtgtgctcg aaggcgagcc
    21361 cgaccaccgc gacatgtact tcaccgacga ggagaaggcg aagaacgacc agttcacgcc
    21421 ctgctgctcg cgcgccaaaa gcagaaccct ggtgctggac ctgtagaccg gccatcgacg
    21481 gttgtgccct aatatcattc accttcccct gcccgatttc aacgacctca aaggaaaaag
    21541 agacatgacc cagctccccg cccgttacga ccacgttggc agttttctgc gccccaaata
    21601 cctgctggaa gcccgcgagc agaaggccaa gggcgagatc acgcccgagc agctgcgcaa
    21661 ggtggaagac aaggccatca ccgagatcgt caagttccag gaagacgtgg gcctgaaaag
    21721 catcaccgac ggcgagttcc gccgcaccta cttccacatt gacttcctcg agcagatcgg
    21781 cggcgtgaaa accgacattc cggtcaccat catccggccc gacggcacgc aggagctggc
    21841 cccaccggtg atgcgcgtga tcgacaaagt gcgccacgtc aaggacatcc agctcgccga
    21901 cttccagtac ctcaagagcc aggtgagcgc gggcaacacg cccaaggtga ccatcccctc
    21961 gcccaccatg ctgcacttcc gcggcggccg cgccggcatc agccgcgaag cctaccccga
    22021 gctcgacccg cagttttatg acgacgtggc caaggcttac ggcgacgagc tgcgcagcct
    22081 ggctgccgcc ggctgcacct acgtgcagat ggacgacacc aacctcgcct acctgtgcga
    22141 caccaagatg cgcgaagccg cccgccagcg cggcgacgac cccgacgagc tgccgcaccg
    22201 ctatgccggc ttcatcaaca aggtggtggc gcaaaagccc gaaggcatgc tgctggccat
    22261 gcacctgtgc cgcggcaact tcaagagcac ccacgccgcc gccggcaact acgagcccgt
    22321 ggccgaggcg ctgctcaagg aaatgaacct cgacgcctac ttcatggagt acgacgacga
    22381 ccgctccggc gacttcaagc ccctgcgttt cctgcccaag ggcaaaaccg tggtgctggg
    22441 cctggtcacc accaagttcg gcacgctgga aagcaaggac gacctgaagc gccgcatcga
    22501 ggaagccgcg aaatacgcgc cgctggagca gctggccctg tcaccgcagt gcggtttcag
    22561 cagcaccgtg cacggcaacg acatcgcggt ggaagcccag cgcgccaagc tgcgcctggt
    22621 gatcgagacg gcgcaggagg tctggggttc ggtctgaccg gttcgccgac tgctccataa
    22681 agaaaagccg ccggcaactg ccggcggctt ttttaatgtc aaatcggccc atagtccaat
    22741 aaatacgcgc gcaagtagct atcaaataat gagcaaatcg caccctttac cccctggatt
    22801 cgggtctgtg acctccatgt atttcatgct gaaagcgttg ccagttcgcg caatgtattt
    22861 gcgagcggtc tggcgcgctg ctggctgaaa gtgtcgcccg gcaaatggcc tgacagggct
    22921 tgttcgaact cgggattggc aaacagcgct gtgaatcctg ccgccaggta ggcgcgcagt
    22981 tcaggcgctg cggcacggca ctcgcccagc agttcgggcc gccggtctgc cacggtgatg
    23041 atgtcttcaa gatcatggct acccagaaag tctggcctgc catcggcgtc tttgccccgg
    23101 tccttgaacg cctccaactt ggtgccaatg aaaaccgggg cgctaatgag ccgaatattc
    23161 atgccgcttg gcaatgtgac caagtctgca gtctgcaggg ccaatggata ccagcggttg
    23221 gcaaagccca agatgtcctt gaccgtgggc atcaagtcaa gcgtgacatt tttataaacc
    23281 caccggcaaa tgggtgcatt gggccgcatg tcgttgctga agccctgagc gcggacttgc
    23341 ttctcaatgg cgtggtagtc atgcaccgtc aacgcttgcg caacgatgtc aacgtcttcg
    23401 gtgatgcgga tacggtcggg ccgctcctgc gtcagcaaca ggcccgtagc gcaaccaccc
    23461 acaaagatca cacgctcccg tacggggccc aaggcatggg ccaccagttc cagaatagcc
    23521 agattggggt tgttgaatgc cgccatcagc tcaacctttc ttccagcagc ttgcgggcca
    23581 tttcacgttc acgcgcctga cctatgcgca gcgcgtcaaa caaggccagc aactcgtaca
    23641 aagccgggtc ctgcgcggcg gccaaaggca acttggggta cagaggcagc agcgcactgc
    23701 cgcgcaccgg gccgtctgca tggggccaca cgggcggcgt ctcatcagca aacagcactt
    23761 tggatttgag cggctccaca ccataggccg taggaaaacc catggtgatc tcgcccctta
    23821 ccgccggaaa aaagtaaggc gccccctgca acaccatggg cctgaaagcc gccagcacca
    23881 gcgtggggat gtcatttacc tcagccaaca ggcgcgctcc actgagccgc gccatgcagg
    23941 catgggcttc aaactgcgac atgtgcatgg cttgcgccag tgctgtgtag ggcagctttt
    24001 gccccgccag acacaccagc ttgaacgcca tggccaagtc ttgcggtttc agcgaccact
    24061 gcgcatggcc aaggcgggat ttgattccgg atgaactcat caacaaacct tgtattcaat
    24121 atgcatacag aatatgccat gaataatttt aaaattcaac aacaaaatca tgattgtctg
    24181 caatatgcat attgaaatac aagttcccag cagcccaaaa cctgcaaaaa ccacacgcaa
    24241 cacgaaatca gagattgaaa tcgggctcca gcccaataaa tacgggcgcg agcagctatg
    24301 cctttaatag caaatcacgc gccgcccgca gccccgccgg cgcaccacct tgccgcggcc
    24361 ggggcttccc gcagcggcgg tacgggccgg cagcgcccga acgatactca aggcccatgc
    24421 tcaaaccctt gatcccccgc ttcgccgcgc acctgcgtaa aaactggaaa agccatctgg
    24481 ttcaactggc cctgctggtg gcgatcgtgg tggcggtgca ggcctggcag acgcggcatg
    24541 tgccggccgg gccggcgcct gacttggcca tcagcgtgct gcagcccgat ggcacgacgc
    24601 acagcaccac gctggcccag tggcgccagg cccaccccgg ccagccggtc gccctgcact
    24661 tctgggccga gtggtgcccc atctgtcgcg ccgaggaaag cagcgtcacc cggctggcgc
    24721 aggactggcc ggtgctcacc gtggccatgc agtccggccc ggccaccaag gtcgggcagg
    24781 tgctgcagca gcgccagttg ccctggcaaa ccgccatcga tacgcgcggt gagctgacgc
    24841 gcgccttcgg ctttcagtct gtgccctctt ttgtggtgat cgacgggcaa ggccagctgc
    24901 gaagcgccag cgccggctac accaccgagc tgggcatgcg cctgcgcctg tggtgggtca
    24961 ggctgttctg agcccgtccc ggtcgccggc ctggcgccca gcctgtgtga tctgaagctc
    25021 aaggtagccc gtcgccgcat ccggctcgcg ggagaactcc cactccggca gcagcgccag
    25081 caagacctct gccgtggtgc gcaccgtgca gcccggcgcc acgtggccgc ccagcacgtc
    25141 gccagtggcg gtggacagcg ccatgtgcag gtgcgagctg ctgcgtgcgc cctcccccgt
    25201 gccgttgaag gccaccgtgc ccgacaggct caggatttcc atgtcgcccg tcaggcgccg
    25261 cggctgctcc gcgcccgcaa agcgcaggcc cgcgctcgac aggctgccta tgccagacaa
    25321 cacaaacgcc gcgcggcagt tggcgccgcg cacggccgcc tcgatggcgc tgcgcaagtc
    25381 ttcgccgggg ttcaggcgga tggggagggt ttgcatggtt tttattgaat caaattggcc
    25441 aatagccctt actggtattg cgcaagcagc tatcaaatca agagcgattt actgacctca
    25501 cccgccctgc tccgggtcgg tgaggtccgt gtatttccgg ctggagccgc gcgcccggtc
    25561 caccgggtat ttttcggcat tgacagccag cttcagccgc gccgcttcca gcaaatcgat
    25621 ctgcagcttg tcgcacagct gcagcaggta gagcagcaca tcggccgcct cggcggccac
    25681 ctcggctttt ttgggtgcgg ccaggtggcg gctttgttct tccgtcagcc actggaagtg
    25741 ctccagcaac tccgccgcct ccaccgacag ggccgacgcc aggttcttgg gcgaatgaaa
    25801 ctgcgcccag tcgcgcgcct gggcaaagtc gcgcagggac tgggtcaggg aagggaggtc
    25861 gtggttcatg tcttggtatt tccggaccca tgggcagggt cgagtcttgt cgggaagctt
    25921 gaagcgcatg ttatgcgttt cagcccctgc aggcctgttg caggccttca aatagccgcg
    25981 tccatgcgaa ccattcatgc aaaaaacggc tgtagtccaa tcaggacggg cgccggcagc
    26041 tatcaaaaaa agagcgactg gcagacttca ccggcaaacc ggcagtcaag cccatcgccc
    26101 ggttacctct catccgcccg aaacagcatc tcgcgccgcg gcccggccgt tccgtcgcaa
    26161 tccacccccg cgccggccct ggcactccta ccatcgcctc cagtttcaac cccctcaaca
    26221 cccacggagc tcaccatgtc cctttcaccc caccgactct ccctcgtctt ctgcggcctg
    26281 ctgctgacgg cggcccaggc catggcggcg caagccggct ggcgccagat caccattcca
    26341 gccaccgcgg ccgatgccgc gcccatcgtg gcggccctgt attaccccac gcaggcggct
    26401 gcgcgcacga tcgccatggg gccgttcaac gtgaacgtgg ccatgcaggc cgcgccagag
    26461 gccaaggtca agggccttat cgtgctgagc cacggcaccg gcggcagtga acttgcgcac
    26521 agcagcctgg ccgaggcgct ggcgcagcag ggctacctgg tggccgcact gcgccacccc
    26581 ggcgacaact ggcaggaccg ctccttgcgc cagggcagcc tggaacgtta tgccaccgag
    26641 cggccgcggc aggtttcacg cgtgatcgac gccctgctca acgacccgca atggaaggac
    26701 cgcatcgcca gcgacgccag ggggccacgc gtgggcgccg tggggcattc ggccgggggc
    26761 tacaccgtgc tggccctggc aggtgcgcgg ctcgagccgg cacgcttcga cgcgcattgc
    26821 aacaccgagc gcgccgagga cccgattttt tgcaacaccg gcaaagagcg cacatcggcc
    26881 acccaccctg gcgccgcagc gccggccatt ccgacgttga ccgacacccg cgtgcgggcc
    26941 gtggtggccc tggcgccgat gggggccttg ttcagcgccg cttcgctggc gcaaatccgc
    27001 gtcccggtgg cgctgtatga agcgcagcaa gaccgttttc tggtgccgcg ctttcatgcc
    27061 gaatgggtgg ccgcgaatgt gcccggcgta gaaatgcacc gcgtgcccaa tgcctggcac
    27121 ttcgcgtttc aggacatgcc caccatgccc atcccctccg aagacggcga cattggcgcc
    27181 aatccgcccg gttttgaccg cgctgcgttt ctgggccagc tgcagcgcga gcttccagcg
    27241 ttttttgaca aggccttgaa ctgacaggca ccgtgcggcg ccggattcgc ctagcgcgcc
    27301 ggcttggcgt tgtgcgctgc agcagcgcgt atcagcgcct tgaaggccgc ctcgtcaagg
    27361 gcgtagccct cccccagatc aatggcgcgg cgggtgttgc cttccaggct ggcgttgaac
    27421 agctttgaag ggtccttgag cgaagcgcct ttggggaaag tcagcttcac cttgaccttg
    27481 taggtctcgc cggtgcagag aatgccgccg tgcgaccaga ccggaatgct ccacttccac
    27541 tcctcgacca ccgccgggtc ggcctgctgg atcagcgcgc ggacatgggc gagcgtctgg
    27601 ccccgccagt cgtccaggga cttgatcttt gcgtctatga gttgcgacgg ggactccgcc
    27661 gctgtgtcct gcttggtctt gccttgctct ttgctcatga gggtgcctcc ttcttgatgc
    27721 cagacaactg gcgtgtcaca ttttctcgcc cggcaactgg ctggcctgct tgatccaggc
    27781 gacgaactgg gcttcgtcga tctccgcatc ctcgcggatg tcaagataac gcgtgtcctg
    27841 gcttttcgat gcaccgggtg gcatcggcga cagcgactgc ccgcggaaga aggttacctt
    27901 gacctacttc gtgaagcaat gaaagctgag gaaccagccc tcctgccccg gcgtcccata
    27961 aaacggcgag ttccatttga cggccttgcg tacgccgggc acggcagcca ccaccagctt
    28021 gtcgagccgg cgcccgatat cctgcttcca gcccggcatg gccgcgagat aggccaacac
    28081 cggtgcatcg ccttcgccct tggggatctg cgggttgccg ccggaaagca gcaccggctt
    28141 gctggttttc gctgtgggtt gcggttttgc tttggtcact gcatgcgccc aatggccgcc
    28201 gtggcccagg ccatctcgct gcgtggaata ggcagcgcag cccgcgccac ccggcgcccg
    28261 cgcggccccg ccggcgccgg gctgtctgtg acgcgctggc tctcctgctc gggcgtcatg
    28321 tggtgcggca cgccgccctg tagcctggca taagggtcgg gggaggatgg ggcgaccagc
    28381 agggtgtggt gggcgccgac ggcgcctgct gtaaaggcgg cggccaggac taagtttctg
    28441 cattgcat
//
LOCUS       DAIDFG010000005        25845 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_5_length_25845_cov_18.223265, whole genome shotgun sequence.
ACCESSION   DAIDFG010000005 DAIDFG010000000
VERSION     DAIDFG010000005.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 25845)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25845)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..25845
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5_length_25845_cov_18.223265"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         4554 a         8677 c         8274 g         4340 t
ORIGIN      
        1 gtgttgctgc gtgatgcgcc cgcgggcctg gaggtgttcc ttatgaaacg ccatggcctg
       61 tccgacgtgc tgggcggcgc ctatgtgttt cccggcggca agatcgatgc cgccgacgcc
      121 gagctggaca tggccgccca cctggaccag ccgctggacc tgctgcatgc cgggctgaac
      181 gagcctggca tcgacctgcg cacggccggc gccgtgtatg tggcggcgct gcgcgaggct
      241 tttgaggagt cgggtgtgct gttcgcgcag ggccaggccc tgcccggcct ggcggaccgg
      301 gccgccgcgc tgctgcgcga aggccacggc ttcaatgccg tgctggccca catggccttg
      361 cgcctgcaga cactcaacgt cgtgccctgg tcacgctgga tcacgcccgt cacgccctcg
      421 gtgaccaaca agcgtttcga cacgcgtttt ttcgtggcgg ccgtgcccgg cggccaggtg
      481 gccagccacg acaatttcga gaccaccgag agcatctggc tgagcccgcg ccatgcgctg
      541 cagcagtact gggatcgcca gatcgagctg gcgccgccgc agatcatgag cctggcgcat
      601 ctgtcgcgcc acgcgggtgt tgacagtgtt ctggccgagg cccgcgggcg ccggccaccg
      661 gtgatccagc ccgagccctt cgaccacgaa ggcaaccggg tgatctgtta ccccggcgat
      721 gcgcgccact cagtgcgcga gcaggccctg cccggcccca cgcgcctgag ctaccgcaac
      781 aggcggttcg aacccgacgc cggcttcgag gccctgttcg gctagccagc gggtgatggc
      841 agccggttgg ttgatggtgc gcagcgcctg gaacgccacc tccgcctcgg gataacggcg
      901 gcgcaaaaag ttgagccact gcttgaggcg gccggcgcgc gagtgcgcat cgagccggct
      961 gcataccagc tcccagaact gggcgatcag cggcagcagc atgtcccagg tcaggcccgc
     1021 gtgccccccg ccgccgcgga tggccagcgc cagacccggg tcggccaccg caccgcggcc
     1081 cagcatcagc atctcgcagc ccgaggcctg ccggcacagg cgtgcatcat ccactgtcca
     1141 gatctcgccg ttcgccacca ccggaatgtc gaccgccgcg cggatgtcgg caatacgttc
     1201 ccagtaggcc ggcggccggt aggcctgcgc cttggtgcgc gcatgcacca ccagctcgtc
     1261 ggcgccgctg ccggcaatcg cctgcgcgca gtcaatcgcc ttgctgtcgt cgttgaaacc
     1321 caggcgcatc ttggcagaca ccggcaggtg cgccggcacg gcgcgccgca cggcagcaac
     1381 gatggctgca atcgtctcgg gctcctcgag cagcgcggca ccgccgccat ggcggttgac
     1441 caccttggcc ggacagccga agttgaggtc gatgccggcc ggccccagcg tggcgagccg
     1501 ggcggcgttt tcggccaggc acaccggatc ggaacccagc agctgggcac gcaccggcac
     1561 acccgccagc gtgcgcccgc cggtgtgcag ctcgggcacg atgcgcgtga aaacgcgttc
     1621 gggcagcagt tggtcggtga tgcggatgaa ctcggaaacg cagcggtcaa tgccgccggc
     1681 ccgcgtcagg atgtcgcgca gcacaaagtc gagcagccct tccatgggcg cgagcaaaat
     1741 cgtcacgact caggaccgga caggcgggga cagacaggca gaacacagaa gggcatcccg
     1801 gtgttttaac agatgcccgg cgaatgtgaa cgggtcgcag cttctaaccc caccaaaggc
     1861 tgacatgagc cgacatgggg accgtgaccg gatggtccgg tgccacctga atgtccaccg
     1921 gcgtgtagcc cgcttgccgg atggcatgcc gcagtgtgga cacttcggtc tttgcgggtt
     1981 cgatgcagac ccggtgcgag accagatcaa tctcgacctt cgccattttg tcagccgcct
     2041 tgacggcccg ggtgatggcg ttgacgccat gcacactggt catgttttct acctgaaacg
     2101 caatcataaa tttctccggt tattgaaggc ggtgaccttc ggacagtgca ccgcaaacgc
     2161 cagacgtgcc agacgtctat ctgcctcgtc aggcccagcc gccacccagc gcgcgaatta
     2221 accctaccgt ggccaggtat tgcgcggcgc gcacctgcag cgcctgccgg cggttgcgca
     2281 attcgctgcg ctgggcatcc agcagctcca gctggctgac cagcccattg cggtaacgcg
     2341 aatccgacag cacggtggcg cggctgctgg atgccaccga gcgcgactgc gcttcggact
     2401 gctcggccag caggcgcagg gccgagagct ggtcctccac atccctgaag gcgaccagga
     2461 tctgctcgcg gtagttggcc agggcgatat cgagttcggc gttcgcgttc tgcacgcccg
     2521 cttcacgtcg gccaccgtcg aaaatcggca gcgacagcag cgcgcccaca ccccaggcgc
     2581 gggtggacat cctgaacagg tcgccgatct cgggcgcggc ataaccgccg ttggccgtca
     2641 gtgaaaagac ggggaaccag gcggccttgg ccacaccaac acgcgcctgc gccgcctgca
     2701 tgctgctctg ggcggccgac acgtcgggcc ggcgcaccag caccgtgctg ggtacaccgg
     2761 ccggaatcac cggcaaagcc gtggcccatt cggccggcgc gacctggaaa ctggaggcca
     2821 cctcgccgac cagcacggcc agtgcatgtt cgagttcggc gcggcgccgg tccagcgtca
     2881 gcgcctccga ctcggtcgat gccacttcgg tgcgcacacg tgccacgtcc agctcggcca
     2941 cgtcgccttc cctgaaacgc acctcggtca aacgcagggt gtcgcggtag gcctgcaagg
     3001 tgtcccgcac cagcgcgcgt tcggtgtcaa gggcgcgcag gttgagatag gtctgtgcca
     3061 cgtcggcctg caccagcagt tgcgtgctgc gcagcagggc ctcgcgcgac tgtgcgtcca
     3121 gcgaggccgc atccgaggcc cccgccagct tgccgaacaa gtcgacttcg taggacaggt
     3181 cggcgcccac gctcaccagg gttttgagcc ggttgttcgc gttggcgtcg gtctggcgca
     3241 ccgcgcccgc gcccagtccc agttgtacgg aatggttggc gtcggtagcg cccgcaattg
     3301 cgcgggcctg tgcgaggcga gcggcggcca cctgcacgct gctgttgctg cgtgtggcct
     3361 ggtccaccag ccggtccagc atggggtcgg aaaaaacctt ccaccactgc gcctggaaag
     3421 gtgcagttgg cgaggcggcc acggcctcgc tttcgcgaaa cagggtgggc gtcaccggca
     3481 ggctggatgg atcgatgggc gcggggctgg tggcgcagcc ggccagcacc agcgctgcaa
     3541 gcagcggtgc cagtgcggaa cgggcggaaa aacgaaggcg gctcatggtg ataactcctg
     3601 gatggggcgc ataaaggctt gtttgaggct tgtttgttat tcgtggtgtt tgagggcagc
     3661 ggccggctgc gcatgcagcg ccccgccacc gctggccgaa ctgccggcaa aggcttccac
     3721 gtggggcacc tcgccatgca gcttgagtgg gcggttgccc gtgaggcggc gcagcgcgac
     3781 gtagaacacc ggcgtgagga acagcccgaa agccgtgacg ccgatcattc ccgagaacac
     3841 cgccacaccc atggcatggc gcatctcggc gccggcgccg gtggacagca ccagcggcag
     3901 cacgcccatg atgaaggcca gcgaggtcat caggatgggg cgcaggcgca ggcggctggc
     3961 ctcaatcgct gcctgcaccg gcgtacgccc ggcaaactcc agctcgcgcg caaactccac
     4021 aatcaggatg gcgttcttcg ccgacagccc caccagcacg atcaagccga tctgcgtgaa
     4081 gatgttgttg tcgcctccgg tcagcttcac gcccagcatg gcagccagaa ggcccatggg
     4141 aacgatcagc gcgatggcga tgggaagcgt caggctttcg tactgcgcgg ccagcaccag
     4201 aaagaccagc agcagcgcca gcgggaacac ccacatggcg gagttgcccg caatgatttc
     4261 ctggtaggtc aggtccgtcc actcgtagct gatgccgggc ggcagggttt ccgctgcgat
     4321 acgggcgatc gcatcctgcg cctggcccga cgaatatccg ggcgcggcgc cgccgttgat
     4381 gtcggccgag aggaagccgt tgtagcgcat ggcgcgttcg gggccaaagc tgggcgtgac
     4441 tttcatcaat gccgacagcg gcaccatctc accggtggtc gagcgcacct tgagcaggcc
     4501 gatgtcttcg gcgcgggcgc ggtaaggcgc gtcggcctgg acccgcacgc tgtaggtgcg
     4561 accgaacttg ttgaagtcgt tggcgtacag gctgcccagg tagatctgca tggtgtcgaa
     4621 cacgtccgtc accggcacac cgagctggcg cgccttggtg cggtcgatgt cggcgtagag
     4681 ctgcggcaca ttgacctgga agctcgagaa catgccggcc agctcgggcg tctggtaggc
     4741 cttggccatg aaggccttgg tgaccctgtc cagctcggca taaccgagcg aagcgcggtc
     4801 ttccagttgc agcttgaagc cgccggtggt gcccaggccc tgcacgggcg gaggcgggaa
     4861 catcgcaata aaggcttcct ggatgccgcc gaattgggcg ttcaactgac cggcgatggc
     4921 gccggcgctg aggtcggcgg tggtgcgctg gtcaaacggc ttgagcgaga caaacacgat
     4981 gccggagttg gagctgttgg tgaaaccgtt gatcgacagg cccggaaagg caatcgcgtg
     5041 ctccacgccg ggttgtttca tcgcgatatc accgatgcgg cggatcacgt cttcggtgcg
     5101 gtccagcgtg gcgccgtcag gcagctgggc aaaaccgatc aggtactgct tgtcctggcc
     5161 gggtacaaag ccgctgggta cggccttgaa gaggcccgcc gtggccagca ccagcaccag
     5221 gtacacgccc agcatcacgg ccttgcgcga gattgcgccc ttgacgcccc ggctgtagtt
     5281 ttcagaaccc cggctgaaca ggcgattaaa acccgcgaag aagcggccga acaccttgtc
     5341 catgccgcgt gtcagcgcgt ctttgggtgc gtcatggctc ttgagcagca aagcggccag
     5401 cgccggcgac agggtcagcg agttgatggc cgagatcacg gtggagatcg cgatggtcag
     5461 ggcgaactgc ttgtagaagg tgcccgacag accggtgatg aaagccagcg gcacgaacac
     5521 ggcgatcagc accagtgcaa tggcgatgat gggcccggac acctcgcgca tggcgcggta
     5581 ggtggcgtcg cgcggactca ggcccgcctc gatgtttctc tccacgttct ccaccacgac
     5641 gatggcgtcg tccaccacaa tgccgatggc cagcaccagc ccgaacaggc tgagcgcgtt
     5701 gatcgagaac ccgaacaggt gcatcaccgc gaaagtgccg atgacggaaa tgggcacagc
     5761 cagcaacgga atgatggagg cgcgccaggt ctgcaaaaag atgatcacga ccagcaccac
     5821 cagcgccacg gcttcaagca gcgtgtgcac cacagcctcg atggaggagc gcacaaactg
     5881 cgtggtgtcg tagacaattt catagtccac gccctcgggc atgctctttt tcaggtcttt
     5941 catcacggcc tgaacgtcgt ccgagatctg gatggcgttg gagcccggcg cggcaaacac
     6001 cggcacggcc acagcctgtt tgttgtccag cagcgagcgc agggcgtagt cggatgcgcc
     6061 cagttccagg cgggagatat cccgcagtcg cgtcacggcg ccgcccctgt cggtcttgac
     6121 gatgatgtcg ccgaactctt cttcgctttg caggcggccc tgcgcattga tggacagttg
     6181 catgtccacg ccgggcaggc cgggggatgc gcccaccaca ccggcggctg cctggatgtt
     6241 ctgctcgcgg atggcgcgca ccacgtcgcc ggcggacaaa ccgcgctcag ccaccttttg
     6301 cggatccagc cagacgcgca tcgaatagtc gcccgagccg aacagctgca cctggcccac
     6361 gccgttgatg cgcgccaggc ggtccttgac attgagcacc gcgtagttgc gcaggtaggt
     6421 catgtcgtag cggtcgttgg gcgagagcaa gtgcaccacc atcgtgaggt cgggtgagct
     6481 cttgatggtc gtgatgccca ggcggcgcac ttcttccggc aggcgcggct cggcttgcga
     6541 gacacggttt tgcaccagct gctgggcctt gtcggggtcg gtgcccagct tgaaggtgac
     6601 gttgagcgtc ataacgccgt ctgtcgtggc ctggctgctc atgtaaagca tgccgtccac
     6661 gccattgatg gactcctcca atggcgtagc cacggtctcg gcgatcacct tggggttggc
     6721 gccggggtag ttggcgcgca ccaccacagt gggcggcacc acatcggggt attcggcgat
     6781 gggcagcgaa cgcagcgcga tcagtccggc aatgaacatc agcagcgaca acacgccggc
     6841 aaaaatcggc cggtcgataa aaaatttgga gagattcatg gcaattcttc ttggtcaggt
     6901 aaaggcaggg gcaagcgctg ccctcaggac ttcgccgcta ccttgacggg cttgtcggca
     6961 tgaaggagct ccgccttggc agacatttcg accggctgcg gggcgaccag tgcaccaggc
     7021 cggacacgtt gcaggccgtt gacgacgatg cgttcgttgg gcgccaggcc gttcttgacg
     7081 acacgcaggc cgttgaccga agcgcccaac gtgacttcgc ggtacgccgc cttgttgtcg
     7141 gcgcccacca ccagcacgaa cttcttgttc tggtcggtgc cgaccgcacg ttcgttgatc
     7201 agcagcgccg tggcttgcgt cgcctggccc atgcggatgc gcgcgaactg gccgggcatc
     7261 agttgcccat ccttgttgtc gaacacagcg cgggcgcgta ccgtgccgct tttggcatcg
     7321 acctggttgt cgaccagttg caacttgcct tcaaacggcg tgtccgtgct ggcggccgtt
     7381 cccatctgca ccgggatgcg ctcgagcctg cgttcgccgc cggcgcttac atccttcagc
     7441 gctttggcca ccacctgttc gtcggcatca aaactcgcgt agatcgggct gaccgacacc
     7501 agcgtggtca gcacaggcga acttgggcct gcagccacca ggttgccgac ggtgacctcc
     7561 agtttgccga cccggccgga caccggtgct ctcacctggg tgtagcccag gctcaactgg
     7621 gccgtttgca gcgcggcctg cgtggcgcgc aggttggcat cggcttcacc tttgccgttg
     7681 gtacgctcgt caaactcgcg cctggaaatg gcctgctcgc tccacaaccg ctgtgaacgg
     7741 tcatgctcgc ctttggcctg ggccagtcgc gcctgcgcgg ccgcaacctg tgcgccggca
     7801 cgctccactt cagccgcata gggtgccgga tcaatggtca gcagcaactc gcccttccgg
     7861 accagcgcgc cttcacgaaa atgcactgcc tggagggtgc ccgccactcg cgagcggatg
     7921 tccacgcgct ccaccgcttc cagacggccc gagaactcgt cccaggcggc cacttcggtt
     7981 tgcacgacgg tggctacgga gaccggcatg gcggggggcg cagtggccac atccgcgcgg
     8041 gcaccggagc cggacacacc aaaaacggcg gcagacatgc ccgccatgac cagaatgccc
     8101 agtgtgaccg ccaggagacg gtgcttgttg ttgtgtagtt gaatcaatga catggtgaac
     8161 accttatggg ggaagaagaa aatttggaac tcggcgtccg ggcaacgcaa acatatcgat
     8221 gccccgaccg cgaacaggaa aacgggcaac tggcggctaa ccggccctgg ccatcgggac
     8281 cgcgcagcgc ccgcggttca tggcgtcaac aggaaatccg gtcataaacg tccttgacaa
     8341 tgaaagaagg agtgaacttt caaccttcca acgatggcaa ggtcaagcca taggggccaa
     8401 acccccatac ctcttgggga aatagttttg gcgtgacggt tttctccaaa ggaaaaacag
     8461 aaaaaaggcg gatcaaggga atcaaggacc tgacagcagg ctgctttgca cgttttgggc
     8521 ggcgctcatg ctggcaaaaa aatcaccgaa ctgcttgcgc accaccgccg gccagccggc
     8581 ctggtcatgg cccgcctcca gatagctgca gggccatccg gtcggtccgg gcagcacggc
     8641 ctcgtgggca aacagccccg cagcgcgcag ccgttctgca taggcttgtg tctcatcacg
     8701 caggggatcg tcctcggccg tgatcagcag cgtgggcggc aaacccgaca ggcgaaagac
     8761 cgaacccggc gttgcatacg gatggctggc gtcttccagt cgaggcaggt agctgtgcca
     8821 gccgtcggcc cagatgcacc ccaccggacc ggcatggaca tcgcgcagcg acgccgttcc
     8881 cagacagggg tccagcatgg gtgacagaag aatctggccg gcaagcgcag ggccatgccg
     8941 gtcccgtgca agcagagcca cggccgctgc aatattgccg ccggcttcct cgccggccac
     9001 aaacatcggg gcgtcgtgtc cggccagctt gtgtctggcc ttccaggccc acaccagggc
     9061 gccataaccg gcctcgacag cctgcggaaa aggatgggcc ggcgccagcg gatagtccag
     9121 cgacatcacc accgcaccgc tggccgcgag caagccggcg atgcttgagc caccttcgag
     9181 cgatccgcca acaaaggccc cgccatgaaa gtgaagcacc agcggcgagc catgcgcgcc
     9241 ttgccggcgc ccgtaaaaac gggccgccag cggctcgcgc tgcccgatgt cgatctgctt
     9301 ttccacgcag ggcggcgggg ctttcgggct ggcctgactg gaatgctgct ggcttgtcat
     9361 catctcgaac cgcaagcggc tcctttgttg caatgcagca gaagataggc gcgatcagca
     9421 gatcaataaa taggcaaagc gtgtttactt tgttccagaa atggaacaat cggcccttaa
     9481 acgccccatg tttcgggcta ttcttcgcag cccagctcaa caggacttcc cggatggaca
     9541 agttttctgc catgcaggcc ttcgtgcgcg tcgtggaggc cggcacgttt accaaggccg
     9601 ccgactcgat ggccatgccc aaggccacgg tgacgcggtt gatccagacc ctggaacagg
     9661 agctggacac caaactgctc aaccgcacca cccgccgggt gactgtgacg gccgatggcg
     9721 cggcctattt cgagcgtgcc ggtcgcctgc tgagcgacat ggaagagctg gaatcgagca
     9781 tgtcgcgggc caaggccagt ccgcgcgggc ggctcaagat cgatgtgccc tcatcgctgg
     9841 gcctgaccgt gatcattccg gccctgcccg acttctacgc gcgttacccc gacatccagc
     9901 tcgaactcgg cgtgagcgac cggcaagtcg acatcattgc ggagaacgtg gactgcgtgg
     9961 tccgtggcgg cgagatcacc gaccagtcac tggtcgcccg gcggatcggg gagttttacc
    10021 tgatgtcctg tgcctcgccg gcctatctga agcgccacgg cagaccgaaa caccccacgg
    10081 aacttgagaa caaccacaag ctgatccgtt atgcctcgcc acgcaacggc aagatgtaca
    10141 gcttcgagta ttaccgcgac ggcgaaacca tcgcgctgga cggccgccac ctgctgtcgg
    10201 tcaatgattc gcgagccggc gtggtcgccg cgctggccgg tctgggcgtg ctgcacacag
    10261 cgaccttcct ggtgcaggat cacatcagca gcggcgagct ggagccgctg ttcaccgagt
    10321 ggtgcggcgg ggccgtgccg ctgcatgtgg tgtacccgcc caaccgccac ctgagcaaca
    10381 aggtgcgcgt cttcgtggac tggatcgccg agctgtttgg caaacacgac ctgatccagc
    10441 gcaagagcac cctccccgtc cgcgcagtac gcgcgccagc cgccgtggtg tagggcctga
    10501 caccgcaggc actgccgccc ctgcacgctg gtcctacaag ggacgcgggt acgtcttaca
    10561 tgccgcgctg cacaagaggc ctacattggc tgcttcattg aaggagtccc catggtccgg
    10621 cttgacgcct cccgtactgc gcgcagcacc ccccgaagtc ttcccgtcac cccgcaggcg
    10681 gacgaagccg ctgcgacgcc ccacaagggc tggccctttg ccgaaggatc gcaaaaccac
    10741 ctggatgaaa cctcggcacc gtggccgcgg ccatcggcca ggccgtcttc tggccagccc
    10801 gaccggccgt cgccacaaaa ataagcggcc gccgacttgg aacaaggtct cagtagctgc
    10861 cgatgtagtc cttcttgccg atctccacac cgctatggcg caggatggca taggccgtcg
    10921 tggtgtggaa aaagaaatgc ggcaagccgt agttcagcag gtaggactgg ccgctgaaaa
    10981 tcttttcctt gggcgtgccg gcctgcgtca cgatctggcg cgcttcgctg ccgtcgatct
    11041 gcgccgcgct gaggctgtcg atgaacgcca gggttttggc aatacgcgcc tgcaggtcgg
    11101 cgaaactttc ttccttgtcc tcaaaacctg gcacctccac acccgcgatg cgtgccgaca
    11161 cactttttgc aaaatcgcag gccacctgca cctgacggag caggggaaac atgtcggggc
    11221 aaagccgcgc ctgcagcaag gcagcgggcg tgatattgcg ggccgtgccg tgtgcctcgg
    11281 ccttggccag aacggcgctc aggctttcga gcatctgttt gaaaacgggc acggaagcct
    11341 ggtacatcga aagcgtcatg gttcaaccct aaaaaaagga aaaaaaagaa aattgaatgc
    11401 ggggccggtc aaacggccgg gtccggcaag ccctgggacc ggcgtgctgc ctgcggctgg
    11461 cggcacagcg ccgccatatt gtgggacagc ggatcgtcgg ggtactcgcg ctggatttca
    11521 gtatagcggt gcagggccgt ggcgaaatcg ccctggctga aggcctggta ggcatgctgc
    11581 gtcagcacgc acaggcggat ctgctcaggc gtggcttcca gttcgggatc gcccccgcgt
    11641 atgccgacca gctcgtagat ttccatgtcg gagcgccggc ccttgacggt caccacattg
    11701 atggggcgca gccacagatg ctcgcccgct tccttgaaca ccgtctggct gatgcagacc
    11761 tgggtgccga actccttgtt cgcgccttcg agccgcgagg cgaggttcac accgtcgccc
    11821 atcaccgtgt agctcatgcg ttcggccgag ccgatgttgc cgaccagcac cgaatcgctg
    11881 tggatgccga tgcgcacatt gagcggccgc aggccctcga gcacccactg ctggttgagc
    11941 ctgcgcatgc tcgcctgcac gcgcagggcg gccacacacg cgtggtaagc gtggtttgtc
    12001 aaaatggcgg gcgcgcccca gaatgccatc acgccgtcgc cgatgaactt gtcgatggtg
    12061 ccctgctcgt ggttgaccgc gcgcgtcacc gcttcaagat aggccgacac ccgcagcagc
    12121 agctcctgcg ccgggctggt ctcggccagg gtggaaaagg attcgatgtc ggaaaagaaa
    12181 atcgtcagga agcggctgct gccgcccagt tcgagccgct ggttcgagct cagcaactgc
    12241 tgtaccaagc ctttgggcac aaaagccgaa aacgcgtgga tggtcgaatc cagcgtgttc
    12301 accgcgtgga tcaacgagct gatttccggt atgcgggagt gaatccgccg ggaagggctg
    12361 gagacaaagt tctgcacatc gatcaccttc ttttccagct gctcgatggg cctggcaatc
    12421 agcatggata aaaagtagat cagcaccacc tgcaaggcca ccgcgagcgc gccgaaaatc
    12481 agcaccttgc ggttgttctc tgtccacttg cgcgagaaat cggccagcgg cgcgatgatc
    12541 atgacgcgcc aggccttgcc gaccgtgggc gggaatttgg caaacatcgc cacgtactcc
    12601 ctgccgctgt gcgcatcgac aaaggtgaaa cgttcgctgt ccttgtcggg ccgtgacgcc
    12661 acggccagcg ccggcagttt gctcgacagg cgcgacaggc tgttttgcag cagcttgccg
    12721 tcctcgcgcc ggatcgcctg gctcgcgtcc ggatgggcaa tgatgcggtc gtcgtcatcc
    12781 acaatcaggc tcagcgaatt cgggctgacc ggccgcgacg cgagaaactt cgacaagctg
    12841 tccagcgtga tgtccgccgc caccacaccg gccaccttgc cgccggcaaa aaagggcatg
    12901 gcaatggtga tgccgggcag gccggtggtt gcaaagatgt aggggtcgga caccgagagc
    12961 cggcgccccg ccgccaccga gtccttgaac caggggcgca cgcgcgggtc gtagacggcc
    13021 gccgcctcgg aacgccctac cggctggccc tgcgcatcaa aaaatacata acggtcctgc
    13081 gccgggccgg cggccttgcg gtccagccag cgcgtggcca gccgtgcctg cgcaggcagg
    13141 gccttgtcct ggatgcggcc gcccgtcggc agcaccaggc tcatgcggaa ggagccgtcg
    13201 tcgaatgcaa catacacgct gctcatgttg ctgctgtgcg ccagcatctc ggtcatgtag
    13261 gcggcagccc gctcctggcg gaaaaaatca ggctcggctt cccccaggct ggccgccacc
    13321 ttgaccagcg acttgatcgg gtccaccagt tcggtcgcgc tggtgatggt ctcgaaacgc
    13381 gctttttcta tcagctggtg ggcggtgtcg cgcgcgatgc tgtcattggt ggcgtagttg
    13441 acccagacag tggcgatgaa caatggcagc gacagcagca gaaacagcac cacgatgctg
    13501 ggccgcaggc gcagcgcaaa ttttccggga taaaccatga tgtgacgtcc tcctcgccgc
    13561 gcgagctggt atttttttat cggaggaggc ccttgttgcg cggctccgcc tgcccgcctg
    13621 cattatggcg attgcggccg cccgttctcg ttcaggccgc ggaccggccc cgcatacgct
    13681 ggaccagcgt cacggcgccc agcaccagcg caccggcgac catgcccacc agcgcgtcgg
    13741 ccagcaaggg ggtgaccgcg cccaacacgc tgccggcgcg cgtggccagc gcttcgatgg
    13801 catggtccag cgcaggaacg ccgtgtgtga ggatgccgcc gccgaccaga aacattgcgg
    13861 ccgtgccggc cactgacagc gctttcatga gccagggcgc cgtcagcaca atgccgcggc
    13921 cgaagctgcg ccgcaagcgg gccgccccgc tgtcgccagt tttctggctc aggtagagcc
    13981 ccgcatcgtc aagcttgacg atgcccgcca ccaggccata cacccccacg gtcatgacaa
    14041 ttgcaatccc gctgagaacc gtcacctggg tgatgaaagg actggcctgc accgtgccca
    14101 gcgtgatcgc aatgatttcg gccgagagga tgaagtcggt ccgtatcgcg cccttgatct
    14161 tgtccttttc gaccgccacc aggtccaccg aggggtcggt cagcgccccc agcaattctt
    14221 tttcacgggc ctcgtcttcg gcccggctgt gcagaaattt gtgcgccagc ttctcgaaac
    14281 cctcataaca gaggaaggcg ccgccgacca tcaacagcgg cgtgacggcc cagggcgcaa
    14341 acgcgctgat ggtcagcgcc accggcacca gaatcgcctt gttcaggaaa gaccccttgg
    14401 ccaccgccca caccaccggc agctcgcggt ccactttcac cccggagacc tgctgggcgt
    14461 tcagcgccag gtcgtcaccg agcacgccgg cggttttctt ggcggccacc ttggtcagca
    14521 gggccacatc gtcgagcacg gtggcaatgt cgtcaatcag cgcaaacagg ctactggcca
    14581 tgggggtctt cctgaaaagt gggcgaacgg aaaaaacata ttgtcgtccg ggatgtgcca
    14641 tcacggtgcc ggccatcgca aatccgccga gaatggccgc agccatgaaa aaaacactga
    14701 ccgcctacct cgccgccctg ctctttctcg tcgtggccga cggcatctgg ctcgggctgc
    14761 tgatgccaca gcagtaccag gcgtggatag gcccgctgat gcgtgcgcag ccgctgctgc
    14821 tgccggccgc cgcgttttac ctgctttacc cggtcggcgt ggtggtgttt gccgtgctgc
    14881 ccgccctgga gcggtccagc tgggcacatg cggtcgccct gggtgcgctg ctgggcttgc
    14941 tggcgtatgg cacctatgac ctgagcaacc tggccaccct gcagggctgg ccgctgcagc
    15001 tgacgctggt ggacatgctc tggggcaccg ccttgacggc agcaactgcc acggcggctt
    15061 tcttcgctgc ccgcgcccgg ggcggagcgc gcgtctaggg tctgcgactc ctgcgactcc
    15121 tggaactcct gggactcttg tatttggccg ctttgcagcc agttgatgca agcgctccac
    15181 ttccaggcct gtccatgccg ctttcgctgt tgctgttctc ttcctttctg catgcctacg
    15241 tgggcatccg actggttccc gccctgggcg gctggccggt cgcgcaactg ctgctggggt
    15301 tggtcctgct ggcctccacg gtgctgacgc cgctcgggtt gacggcccgg cgcttcaagt
    15361 cgcagccgct gtcggaccgc ctggcctgga ccggcctgct ggccatgggc ctgttttcct
    15421 cgctgtttgt gctgacgctg ctgcgcgacg cggccctgct ggccggcctg gcggtgctgg
    15481 ccgtggcgcc tgcggccttt tcgctcgatg ccgcggcaag aggctcggcc gctgcggttc
    15541 ccctggccgg gctgttcatg agcctggtcg gattcgtcaa tgcgaggcgc acggccaccg
    15601 tggtgcaggt ggacgtgccg atcaccggcc tgcctgccgc cctgcacggc tttgcgatcg
    15661 cgcagatcag cgacatccat gtcggcccca ccatcaagca cggctatctg caggccatcg
    15721 tgcgcgcggt caacacgctg caggtagaca tggtggcggt gaccggtgac ctggtcgacg
    15781 gcacggtggc cgaactggcc caccatgtgg cgccgctggc cgggctgacg tccacccacg
    15841 gcacctactt tgtcaccggc aaccacgagt actactcggg cgcacacgcc tggattgatg
    15901 aactgcagcg cctgggcatc cgggtgctga tgaatgaaca tgtggtgttg cagcacggcc
    15961 agatgcatgc gcagcaggcg ctggtgctgg ccggcgtggc cgactacagt gccgggcatt
    16021 tcgacgccag ccaccgcagc gatccgcatg cggccatggc cggcgcaccg gcgcatgcac
    16081 cggtgcgcgt gttgctggcg caccagccgc gcagcgcgca cgaggctgaa aaagccggct
    16141 ttgacctgca gctgtcgggc cacacccatg gcgggcaatt ctggccctgg aattttttcg
    16201 tgcgcttcca gcagcctttc accgccggac tgcaccggct ggaaaagctc tggatctaca
    16261 ccagccgcgg caccggctac tgggggccgc ccaaacgttt tggcgcgccc tcggaaatca
    16321 cgcacctgcg gctgattcct gtgtgagacc tgccggcgcg gctgatcagt agcggctggc
    16381 ggtcagccga cccagcacaa aaccggccag cacggcaaaa ccgacggcct gcaggggatg
    16441 gtctttgatg taggctttgg aatggtcgat agcttgcaga ggcgcgtcgc tcaggcggtg
    16501 cgcctggtcg ctgaacttgc cggccaccga gttggcgccg ctagccagtt tgtccaccgt
    16561 ctcgtgagcg gcgcttgcag cacggtctac ggtgttgcgg gcaggttctg ccaccttgtc
    16621 aatcgtgtgg tgaagcgtgc tgccggcctg gtccacaccg cgcatcaggg cgctttcgct
    16681 cgacgaagtg gcggaaccgg ggtaagcgct ggcggtggat ttttccatgg tgaagtcctt
    16741 tggtgaatgc gatgcgaatc agaaaattct gcgtgtcccc aggtcatggc ggggcatggc
    16801 ctggggcgca cagtgcactt tcccgcatgc agcgcccgca cggtgtgcgg agggggaggc
    16861 gcctgctgta ggacaaggga gccagtgggc ctggcccgcg ctgtccgggg tccgctgacg
    16921 ggcgagtcag tcgaacaggc cgggcgtgcc gcgcatggcg ccttcgatga gcagcagcct
    16981 gagcttggtg cggatgccgc cgctggctga gaagccgccc ggcttgtcac cggccgccag
    17041 cacgcggtgg caaggcacca cgggcgcaaa ggggttgcgt cccagcgcct ggcccaccgc
    17101 acgtgccgcg ccgggttcgc ccaggcgggc cgccacctcg ccgtaggtca gggtctggcc
    17161 cggtgcgatg tcgcgcgcca cggcatacac acgctggtga aactcgggca caccgcgcat
    17221 gtcgagcacc agcgtgctga agtcgtgcgc ctgcccgcgc agcatcgcca ccacgcctgc
    17281 aacggcggcc tgcacgtcgg cgggcggcgg cgcctcctgc ccgtcgggaa aaccctcacg
    17341 catgcgatcg cgggtctgct gcgccccgcc ctcgggcaat tgcacaccct ggatgccggc
    17401 gtcgccccaa gcgatgccgc agcagccgat ggcggtgtcg aacaatgcga aaaaaggggt
    17461 ggcggtggcg gtgcgcatgg aagcagtcat cgtaatacag cgcgcagccc ggtcacgccg
    17521 ggcgccgccc tgctggccgc acggcgctat tcaatgcata gcaggttgcg caatacagac
    17581 aagggctgga ggccaaaatc gtgcataaaa accagcccca agcgctgcgc cggtgcgccg
    17641 ctctgtcggc ctgcgctgac aggcgaacaa ggttttttcc aaacgctggt tgtggcaaac
    17701 ggcgcaacat gggcctccac cacaacccag cggagcacat gatgccctca tcgatcgcca
    17761 gcgacagcgg acgccgtcac caggacagcc ccgaggaaac caccggccag gacgacaccg
    17821 acgacctgcc gctgactccc gaagaagaca tgccggtgat tccggacgac gaacgcgtga
    17881 tcgacgtgcc gtcctgaacc cggcggcgag ccgcttcagc ccacgtcgcg cttgaggcgg
    17941 atttcctcga gcttgacgga accgatcacc acggtcttga gcgaggtcat ctcgcgcttg
    18001 aagcctgcgt gttcatgaaa acgcagtgca cgctcgttgc gcaccggcac ccagatggtg
    18061 accttggtgc agccttcttc cttgaggccg tcattggccg catcccacaa ggccacgccc
    18121 acgcccttgt cccaatgcgc cgggtccaca aaaatggccc agatctcgcc gcaggtggac
    18181 ggtgttccct tgtcgcgcga acggtcaaag ccgacaaacc cgatcacacg ctcgccgtcg
    18241 gtggccacat gcacctgtgg ctccatgtat tcgatggcat cgcgccagaa ctgctggcgc
    18301 ttgtccaccg ccagcgtgcc cagctggtca tcgggcacca gccccttgta ggcggcgcgg
    18361 gaagagacgt cgttgatttc ggcgatggct ttggcatcgc gcacggtggc ggggcgaact
    18421 tggtagctgg acatgaaaaa agacgcgctg aggaatcgtt gttgggggac ccggattgtc
    18481 gcagcaaata cgtccaggcc ccctgctgca ttcagatcgc attcataccg cggtcaagcc
    18541 accatcgacc gtgtgggcat gcccggtgac aaaagaggcc gcgtccgaac tgagccacag
    18601 caccacggcc gccacttcat cgacctcgcc caggcgcccg agcgggtgca tctgcgcggc
    18661 ggccacgccg atctgcggat cggccgccac ggcgcgttcg tacatcgcgg tgcggatcac
    18721 gccggggcat acggcgttga cgcgtatgcc cttgcgcgcg tattcgatcg ctgccgattt
    18781 cgtcaggccg agcaccgcgt gtttgctggc gctgtaggct gccatcttgg gcgcgccgac
    18841 caggccggcc accgaggcgg tgttgacgat ggtgccgccg ccctgttgct gcatctgcgc
    18901 aagctggtac ttcaggcaca accacacgcc cttgacgttg atgcccatga tgcggtcgaa
    18961 ggtggcttcg ctgctgtcgg ccaggcgcag atgttcttcc tcgatgccgg cgttgttgaa
    19021 agcgcaatcg agccggccat aagtggccac cgtgtgggcg accatggcct gcacctggtc
    19081 cgcctgcgtc acgtcggtgc ggatgaaact cgcttcgccg cccgcggccc gcaccagcgc
    19141 caccgtctcc tggccggcat cggcggcgat gtcggccaca acgacacggg cgccggcccg
    19201 cgcaaatgcc agcgcggtgg cgcgccctat cccgccgccg ccgcccgtga cgagggcgac
    19261 cttgtccttg aatgatgttt gcacctgagg gttcctaaaa cgccaaaagg gaaaatcata
    19321 cggacggcca cgccggaacc tgtcccgcct gggaccggcc gtcgcctgcg cggcagcgcc
    19381 taaactggca ccatgcccgc cacccccgac gaccacgccg ttttccaggc ccgcctggaa
    19441 tctgagggcc cgcccttcgc tgcgctcgcc tcgcagcccc tgctgcagtc ggcactgcag
    19501 gccggcatcg tgcttgaaag ctcctgccgc aacggcacct gccgcagctg catctgccgc
    19561 ctggccagcg gccgggtggt ctaccgcatc gagtggccgg gcctgagtgc cgaggaaaag
    19621 gccgacggct acatcctgcc ctgtgtcgcc tacccggtgt ccgacctggt gatcacgcgg
    19681 ccggtctgaa cgccggccaa aaaaaatgag cagaatcggc ctccagccca acaagggcgg
    19741 gcgcaagcag ctatgttttt catagctaac ccaagccagc cacaaccggt tgcgccgcac
    19801 atcgttttgt cacaccgctg tgtcaggatg gagacatgga ctttctgtgc ccgcctgccg
    19861 gcggctttga cctctcccgt ctggcggcca cccccgacag cgccgccacc tttgccgaac
    19921 acgaagagct caaggccatc ctgctggccg gcagcaccca tttcgacatc caggccgtcg
    19981 gcgaaattgc cacccgcggc ccgtctttcg cgctgtacac cgccggcatc ggttcgcgcg
    20041 acccgcgggc acctgccgtc ggttttttcg gcggcatcca tggcctggag cgcatcggca
    20101 cccagctgat cctggcctgg atgcgggcgt tgctgtttcg cctcgagtgg gacgagctgc
    20161 tgcaccagca actgcggaag gtgcggctgg tgttcatgcc catcgtcaac ccgggcggca
    20221 tgtgggcgca caaacgcgcc aacccgcacg gcgtggacct gatgcgcaac gcaccgcaga
    20281 gagccgaaag ccgcgtgcct tttctcgccg gcgggcagac cttcggcccg ctgctgccct
    20341 ggtattgcgg ccggcccggc gcacccatgg aaatcgaaag cgcggcgctg ttgaagctgg
    20401 tgcgtgagca gctggccagc cggcccttca gcctggcgct ggactgccat tcgggttacg
    20461 gctttgacga cagcctctgg tttccctacg ccaagacgcg gcggctgatg ccgcacctgc
    20521 ccgagatgtt cgcgctcaag accatgctcg agcaatcgca cccgcaccat gactacagct
    20581 tcgagccgca aagcaaccag tacctgctgc acggcgatct ctgggaccac gcctatgacc
    20641 aggcgccggc ccatcacgtg tttctgccga tgacactcga gctcggctcc tggctgtgga
    20701 tacgcaagaa cccgcgccag ttgttctcgc gccacggcat gttcaacccg atcaaggcgc
    20761 accgcgtcaa gcgggtgctg cgccgccatg ccgacctgct ggacttcctc acacgcgccg
    20821 cctacgccgc accgggctgg ctgcccgcgg gcgagcggcg cgcgcagctc gcccaactgg
    20881 cggtggcgca ctggcgccca aggcgggcgc catgagctgg gtcctgttgc gcggcctgac
    20941 ccgcgaggcg aggcattggg gcaacttgcc gcagcaactg gcccgcctcc cggacgccgg
    21001 ccgggtcgtg acgctggacc tggctggcaa cggcgtgttt caccgccagg cgtcgccggc
    21061 ctcggtgcgc gacatcatga cgctggcacg ccgccagctg caggcgcagg gcgttgcgcc
    21121 cccctggaag ctgctggcga tgtcgctggg cggcatggtg gcaaccgact gggcgcagca
    21181 gcatccgcaa gaggtcagcc ggctggttct catcaacacc agcatgcggc ccttcaaccc
    21241 gctgacgcag cgcctgcggc cgacttgttg gccgcaactg ctgcgcatcg ccgcgcaatg
    21301 gaagaatgca gcgggcgtgg agcactgcat ccaccgactg acctgcgcga acacagacac
    21361 cctgcaaacc gatgttgccc tatgggaaca tatccgccgc agcgcgccgg tcagcagcgc
    21421 caacgccctt cgccagttgc tggctgcggc gcgctttcag gcgctgccgt tcgcaccagc
    21481 ctgtccggtg ctgctgctgt cgtcgagggc cgaccagctg gtagacccgg cctgctcggc
    21541 caccttggcc gccgcctggg gcgcccatca tggccagcac ccttgggccg gccacgacct
    21601 gtgccacgac gacccgctat ggacccacca aaccatggcg gactggctga tccctagcta
    21661 gcgcgccggc ggggacccct gcagggcgcg cgtaggcttt agagggacac ggaaatgcta
    21721 ctttttcaat agcatgcagc gcccgtttgg cctggggaaa cgaccatttt tatacaaaca
    21781 aaacacgccc gcgcaggctc cccgccggtt ttgccaatct tgtaaccaga tcgggccatg
    21841 ttgcgctagc gcatggtgag gcagcacggt gggggcatac gattgtcgaa tcagttttac
    21901 agatcatttt tttggagatt gcattgaaac gcgttaacaa actcttcaca gcactggccc
    21961 tcactgccat cagcgtcggc atgagttcgc tcgctgttgc gcaaaatcca cccactgaca
    22021 ctggcatggg cgctgccgcg cctgcctcag cggcttcccc gcccggcgca aaagactgga
    22081 aagcgcccga catcacgaaa ttgccggacg acaagtacgg ccagtcggtg cgccagggca
    22141 agctgctgat ggaggaaacc tacaagcaca tcggacctga agtcaaggat gtgaaaaagc
    22201 gctatgccgg caataacctg gcctgcactt cgtgccacat ggaagccggc gggcgaaagt
    22261 tcggcaaccc gtggatcggc acctttgcca gctttcccca gtaccgcggg cgcgaagacg
    22321 cggtttcgac cagcgaggag cgcatcaacg gctgcatgga gcgcagcatg aacgggaaaa
    22381 aactgccgct ggacagcgaa gaaatgaaag ccatgatggc ctatctgcat tttctgtcga
    22441 ccggtattcc agtgggctcc aaacttgagg gcggtggcac gctcaaactc aaaaccctgg
    22501 cgcgcgctgc cgaccctgtg gccggaaaac aggtttacgc gaacacctgc gtggcttgcc
    22561 atggcgacaa gggccagggc gtgcgccgcg gcaaggcggg cgatgccaac ggttacctgt
    22621 ttccgccgct ctggggcccc gacagctaca acaacggcgc cggcatgggc cggatcacct
    22681 tggccgcagg cttcatcaag ggcaatatgc ccagcggtat cacgcacctg aacgcggtgt
    22741 tgaccgatga gcaggccttt gatgtggccg cgttcatcaa cagccagcca cggccggtca
    22801 agcgcgatct tgaaaaggat ttcccggcac gcaagaacaa gccggtcgat gcggcctttc
    22861 cgccctacac gccgggcttt tcggccgagc aacacaagtt cggtccgtgg cagccgattc
    22921 aggaggcgcg agccaaaggg atttatccgt tgaaatagcg caacgcatgg cgttgacgca
    22981 acgccgcggg acagtcctgc cgcatggcag cgccggcgga gtctgccagc agcttgcccg
    23041 ctggctggca ggtccatgac attgctcctc ctttcatagc agcccgcgct tttgcgataa
    23101 gcgctggcgt cgccctggcc gtcaaagcgc acaggcgtga cacagggtct tgcaggcgcc
    23161 ggccagcagg ccccggacaa gtcagggaaa attcagcttt gcctgccaag ctcagcactt
    23221 ccatgaagac gccaaccgca accgttcaag cccaggagac cgcagcaccc gctgccgggt
    23281 cgattcacgt cgcacccaca cgggacaagc gcatctgggc gctggcgctc ggcacggcgg
    23341 cggtctggta cctggacccg gctttggcag agatggccga actggtgggc ggcatcgcct
    23401 gcgcgaccaa caagtcgctg gtcagggacg atatctcgga catgcgcctc aaggccgacg
    23461 ccatgcgcca gcaggtcggg ccgctgccgg ccagcgcctt ggccaaggtg gtcagacgca
    23521 ccgaggcggc cagcggcgcc gtgctcgaca aaggtgcgcg catgaaagag ctgcaggcca
    23581 gctgggacca cttcctggcc tgcctgcgca gcagccccaa acgctaacct acccgcaggg
    23641 accgcccgct gccgctgcgc tgacttatca ccatcggcaa cgcgtctggg accggcgccc
    23701 ctgctccaag gcatgatgcc tccgtgaaaa catttttatt ccttccttcc accgcgctga
    23761 cccacctgct ggccgcggcc atctggctgc ttgccgggac ggcgggcgcc gcgccttttg
    23821 aaaacaccat cgcccaacgc accatggcct gcaccgcctg tcacggcgcc caggggcgcg
    23881 ccggccccga cggttattac ccgcgcctgg ccggcaagcc cgccggctac ctgtacaacc
    23941 agctgctgaa cttccgcgac ggccggcgcc actacgcgct gatgacacag ctggtcgatc
    24001 ccctgaccga cgactacctg atggaaattg cgcgctactt ttcggccctc gacctgccct
    24061 atccgccccc ggccgcgccg gctgctgcgc ctgacgtgtt gcggcgcggc aaggcgctcg
    24121 ttttgcaagg ggaccccggg cggcgcctgc cggcctgcgt gtcctgccat ggccaggcca
    24181 tgaccggggt cgccccccat gttcccggcc tgctggggct gccgcgcgat tacctgaacg
    24241 cccagctcgg cgcctggcgc accgggcagc ggcgcgccca cgcgccggac tgcatggcca
    24301 acatcgtcgg ccagatgaac gacgcagaca tccatgccgc caccagctgg ttggcggccc
    24361 agcccctgcc ggccagcagc aaaccggcgg caaccctgcc ggccgcgccc gccggccagg
    24421 tggtgccggc gtgcggcagc gccgtcgcac cgcggggcac ccggccatga aacggctgat
    24481 catctggatc ctggtcagcg tcgcagcggc gctggcactg gccgccctcg tctggctgct
    24541 caacgtgcgc gatgaaccca acgtcacgac gcccgtggcc accgcggccg cgctgccggc
    24601 acggatagca cgtggcgcct acctcgcgcg cgcaggcaac tgcatgagtt gccacaccac
    24661 acgcggcggc acgccgtacg ccggtggccg cggcatagac acaccgttcg gcaccgtttt
    24721 ttcgagcaac ctgacacccg accgcagcac cggcattggc cagtggtcgc ccacccattt
    24781 ctggcgtgcc atgcacaatg gccgctcccg ggacggccgc ctgctttacc cggccttccc
    24841 ctacaccaac tacaccgaaa tcacgcgcga agactcggac gcgctctacg cctacctgca
    24901 cagcctgccg ccggccacgc agcccaacac gccccatagc ctgcgctggc ccttcagcac
    24961 gcaggccgcg ctggcggtct ggcgtgcgct gtacttcagc ccggcgcgtt acgcgcccga
    25021 tgcccaacag acggccgagt ggaaccgcgg cgcctacctg gtgcgcggcc tgggccactg
    25081 cagcgcctgc cacagcacgc gtaatgcgct gggcgccacc aacaccccgc tggacctggc
    25141 cggcggcctg atcccgatgc agaactggta cgcgccctcg ctgacctcgg cgtcggaggc
    25201 cggcgtggcc gactggacgc cggaacacat tgcggccctg ctcaagaccg gcgtgtcgcc
    25261 gcgcggctcg gtgctcggtc ccatggccga ggtggtgctg cacagcaccc agcacatgag
    25321 cgatcaggac ctggcggcca tggccgtgtt cctgaagtcg ctgccgcctg cgccggtggc
    25381 ccgcacttcc accgcagcgg cagcccgcct gccggctgcc ggcgccaagc tgtacgagca
    25441 gcactgcgcg cagtgccacg gcgatcaggg ccagggcgtg ccgggcgcct acccgccgct
    25501 ggccggcaac cgggccgtga ccatggactc caccgccaac ctcgtgcaga tcgtgctcaa
    25561 cggcggttac gccccggcca cagccggcaa cccgcgtccc ttcggcatgc ccccgtttgt
    25621 attggtactc gacgacaccg aactggccga gctgctgacg tacctgcgca gcgcctgggg
    25681 ccaccaggcg agcgcggtga cgcctaccga gatcaatcgc atgcgggggg aaaatcagcg
    25741 gtgaaggttg gctgcctttt atgtctgctt gatctggggt agtgccgcca gctagccggg
    25801 tctcgccccg gcgggcgact tacttttctt tgcttcgcca aagaa
//
LOCUS       DAIDFG010000006        25384 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_6_length_25384_cov_18.396699, whole genome shotgun sequence.
ACCESSION   DAIDFG010000006 DAIDFG010000000
VERSION     DAIDFG010000006.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 25384)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25384)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..25384
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6_length_25384_cov_18.396699"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         4442 a         8429 c         8441 g         4072 t
ORIGIN      
        1 accgacgtgg tgtgcaccgc cttgccacgg cgcagcacgg tcacgtcgtc ggcataggcc
       61 atcacctcgc ggaacttgtg cgtgatgatg agcaccgtgc acagcccgct gcgcgcgaac
      121 tcgcgcacat ggccgagcac ctcgtcggct tcctgcggcg tgagtaccga ggttggctcg
      181 tcgagaatca gcaggcgcgg ccggaggtag agctgtttga gcaactcgag cttctgcttt
      241 tcgcccgccg ccagctccga cggccgcgcg tccaggtcca ggcggaaagg cgtgtgcgcg
      301 agaaaggtct tgagttcatc acgcttggcg cgccagttga tcaccgcggg caactggccg
      361 ccggccagca acaggttctc cgccaccgtc atgcccggtg ccagcgtgaa atgctggtag
      421 accatgccga tgcccagggc gcgcgccacc accgggttgt caatcgcctg ctcgcggccg
      481 tcaatcagga tgctgccttc cgaaggcctt tgataccccg ccacacattt gaccagcgtg
      541 cttttgccgg caccgttttc gccgagcagc gcatgtaccg tgcccggcgc caccttcagg
      601 ctgacactgt cgagcgccgt gaagctgcca aaacgcatgc tcatccggta ggtatccagc
      661 gccagcgcac cggtggccgg cggcagactg ccgatggctg cggtggtact catggctggg
      721 ccagtgcgtc cagcactgcc tttgactccg catgggcgcc gaacaccccg ccttgcatgg
      781 tgatcatctt cagcgccgcc gcgtggttgc cggcgtcggt ggcggcggtg cagtccgaca
      841 gcagcaggca ttcgaagccg cggtcgttgg cgtcgcgcat cgtggtgtgc acacacacgt
      901 cggtggtgat gccggtgagg atcaggttgc ggatgccgcg cgtgtgcagc accatctcga
      961 ggtcggtggc gtagaacgat cccttgccgg gcttgtcgat caccacctcg ccgggcagcg
     1021 gcgcgagttc gggaatgatt tcccagcccg gttcaccgcg caccaggatg cggccgcagg
     1081 gtccgtcgtc gccgatgccc acacccttcg tgccgatctg gcgcgagcgc cagcgtttgt
     1141 tggccggcag gtccgacagg tcgggccggt ggccctcgcg cgtgtggatg atgtggtaac
     1201 ccgcgccgcg catggcggcc atcagggtct tgagcggctc aatcggtgca cgcgtcagcg
     1261 agatgtcgta acccatcttg tcgacgtaac cgccttcgcc gcagaaatcg atctgcatgt
     1321 cgatcacgac aagcgcggtg ttgccgggct gcaggtcgcc gttccagggc caagcgtacg
     1381 ggttggcgtt gacgaagcgt ggcatggtca tgttttcctt ttcaggcctt gtagctgcgc
     1441 gtcggcgcgt cgaagccgca ggaggtcccg tcggtcacct ccaccgtcca gggcagctgg
     1501 taggtgccgg ccgccatgtc gcgcatgtag gtgtaggggc agtcctgggc gccgcccttc
     1561 actgccacgt agccgcggtg gtagagctgg tagatgttgt tttccacgcc ccagccggta
     1621 cgggcctcgc gcaccaggtc gggccgcact tcggcggtga tgatttcatc gggccggccg
     1681 ctgcccgcca ccagcaccgt gccgtcgaaa ttgcagaaca tgccctcgcc catggaatcg
     1741 aaggtgccgt cgctgccgca catgcagaca ttggcggtga ccgccaggtt gcagaaggcg
     1801 ttggcctggt tggtgatttt ccagctgtga cggatagggg cggtgtaacc cgccgtgcga
     1861 atgatgattt cggcgccctt gtaggcggcc tcgcgcgcca tctcggggaa catgccgtcg
     1921 tggcagatga tcagcgccag cttgctgccg ttcgggccgt cgcagacggg aatgccgagg
     1981 ttgcccggct cccagggctc gaccggcacc cacgggtgca gcttgcggta atacagcttg
     2041 accgtaccgg tgtcgtcgat gatcaggccg ctgttgtagg ggttgccgcc ggggttggcc
     2101 tccatgatgg agaaacatcc ccagatgtga ttgtccacac aggcgcgctt gaaggcggcg
     2161 acctcggggc cgtcgagcga caccatcagt tcgggcgcga tcgacatcga caggccgtgc
     2221 agcgaatatt ccggaaacac caccaggtcc atggtggact ggttgcgcct ggccttggcc
     2281 accatggcgc agatgcgctg cgtctgcgca gcgagctgcg ccggtgtctc gaccacgggc
     2341 agctgcaact gcaccaggcc caaaaccacg ccatgtttcg atttgttgag tccgcccagg
     2401 cctgtcatca tgtttccttg cttgtgttca atggttggag gagagttcgc ccgggctgcc
     2461 gctcaccgtg gtgccgggcc ggcaggtcat gaccagaatc agcagcgtca gcgcataggg
     2521 aacaatgttg aacaggtggt agccccagct gatgcctatg gactgcaggg caggcccgat
     2581 ggcgccggcg ccgccgaaca gcaaggacgc gtagacgcag cggatcgggc tccagcgcgc
     2641 gaagatcacc agcgccaccg cgatcaggcc ctggccgctg gagacgccct cgttccagct
     2701 accggggtaa aagagtgtga ggcaggcgcc gcccagaccg gcgaccatgc cgccggctgc
     2761 ggtggccgca atacggatgg ccgacaccga atagccgagg gcgcgcgtgg cgttggccga
     2821 gtcacccgcc atgcgcacca gcaggccggc gcgggtgttg gcgaaagccc accacagcgc
     2881 cacagccagc gcgatgccga tgggtaccag tgcgttgacc tgcaaggccg aacgcacggc
     2941 gcccgaatcg ctccagttgc ccagcgcgat ggccggcagt tgcggcgcct gcggctggat
     3001 caacggcttg cccaggtaaa acgccaagcc catgcccagc agcatcagcg caatgccggt
     3061 ggcgatatcg ttgacgcgcg gcagcgaaca caacacgccg tgcagggtgg ccagcagtgc
     3121 gccggtggct gcggctgcca acacgccgat ccacggcgac ccgctcagcc aggaggcgcc
     3181 gaaggccgac atcgccgaca gcaccagcac gccttccagg cccaggttga tgcgtccgga
     3241 tttttcggtg atgcattcac cgaggctgac aaacaggaaa ggcgcgccga cacgcagtgc
     3301 gccgccgacg atgccggcaa agaagatcag cagctggtcg gttgtcatgg cttgtcctgt
     3361 ggcaagggga tcggactcgg ctcggacgga ccgaagcggt tgatgcgtgc gagcaaggct
     3421 ttccagtcca catcacgcag ggcctcgctg gccaggatca gcacaaaagc aatgccctgc
     3481 agcaccagca ccgaagcgtc gggcaaaccc aggcggcgct gcagcaggct gccggccgcg
     3541 ccgaaaccgc cgaacaggat ggccaccggg atgatggcca ccggatgctg gcgcgcgatg
     3601 aaggccacca ggatgccggc gtagccgtaa ccggcgatca gcgaggcgtt ggcattggtg
     3661 tgcacggccg cgacctcgac ggcgccggcg agcccggcgc aggccccgcc gagcgcgcag
     3721 gccagcagga tcagccggga tgccggcagg ccgaccagtt gcgccgcgcg cgggttgccg
     3781 ccgaccacac gcactgcaaa gcccgaaggt gtcatgcgaa accacaagcc ggccgccagg
     3841 caggcggcca cgccgatcaa gagcccccag tgcacgtcgg agccaccgat gccggtgatg
     3901 cgcaaaccct cggccagcga cggcgtggac ggcttgttca ggctggccgg gtcgcgcaac
     3961 ggcccttcga ccaggtgctt gaacaggccg atggccacat aagccagcaa caggctgctg
     4021 atggtttcat tgatgccgcg gtactggcgc agcgcaccgg ccagcatgat ccacaacgca
     4081 ccggccaggg caccagccag gcacaccagc gccgttccca cgaagccgga gggcagcggc
     4141 agggcggccg ccagcgccgc gcagccgagc ccgcccagca ccatggcacc ctcgccgccg
     4201 atgatgacca ggcccgcacg cgccggcacc gcaacacaca gagccgtgag catcaggggc
     4261 gcggcgcgct gcagcgtgtt ctgccaggaa taccagtcgc cgaaggcgcc cttgaacagc
     4321 agcagccaga cgtccagcgg attgacaccc gccagggcga cgaacacgcc aaacagcagc
     4381 aacgccgcgg caatggcggc cgtgggcagc aggacgttgg cgagggaatt gcgcatcagt
     4441 ttggtatcag tgatcgacgt acatgttcaa ggccgccgcg gaaccggctt tgccgggccg
     4501 ctggtggcgc ccccttgagg ggctgagagc cgcggctcca agcctgcctg cgcaagcttg
     4561 gacgtgcccg aagggcagcc acctctggtg gccgcaccgg gcgccgcagg cgcttcgggg
     4621 gtgttcgtca aacactgccg atcacgcctt cgaccaggta attcatcttc tccaactcga
     4681 ggtcggtctg cttgtgaacc ttgccggccg ggatgatggt cgcgcccttg ttgtccttga
     4741 gcgggccctt gaagatgtcg aacttgccgg ccaccatgct ggccttgatg ctgtcggcgt
     4801 tctttttcgc gacgtccggc accatcgggc cgtaggcgga catcttgacg tagccgtctt
     4861 tcaggccgcc gcggatgaag ttggggtgcg gcttgccgct gcgtgcggcg tcgaggaagg
     4921 tggtgtaggc ggttagccag ttccactcgg cgccggtcag gtaggcgttg ggcgccagct
     4981 tggcctggct ggcgtggtag ccgcacacca tcttgccgcg tttggcggcc gtctcgacaa
     5041 ccaccttggg accgtccaca tgcatggtga acacatcgca gccctgatcg gccaggctgt
     5101 tggtggcctc ggcctccttg acggccatgg accagtcacc ggtgaagatc acgttgcagg
     5161 tgatgccggg cttgaccgag cgcgcgccca tggtgaaggc attgatgttg cgcagcacct
     5221 gcggaatcgg cttggcagcg acaaaaccga tcttgttgct cttggtcatg tggccggcga
     5281 tcacgccgtt gaggtactgg cactcctcga tgtagccgaa gtagctggcc gcattcctgg
     5341 ggtgtttgcc ctcggtccag agaccgccgc aatgcgcaaa acgcacgccc ggaaacttgg
     5401 gtgccacggc cagcacatgc ggatcaaaat aaccgaagga ggtggggaag accagcgtgg
     5461 cgccgtcctg ggccaccatg cccgtcatgg ttttctggac agcagccgtc tccggcacgt
     5521 tttcttcctc gaccaccttc acgccgggca tcttcttgag ttcggcagcg gcctgggcct
     5581 gggcctggtt gtagccgtag tcgtcgcgcg ggccgacata gatcacgccg acggtgatgg
     5641 gtttttgcgc aaaggccagt tcgctgaagg cgcccagcgt gccggcagcg ccgagcgcgg
     5701 ccagggactt gagggaatcg cggcggctga attgtctggt cgtcatgagg tttctccttg
     5761 ggtcaagtta aagaagtact gaggaacggg aagcaggatt catgcaagca ggctcacatg
     5821 ggcagacacc acgcgccagc ccccgggcgt gcgcagccag gtctggctct gccggccagg
     5881 ccgggcgctg ccggtgcgct ggaactcgac attggcggtc gccatgtcct ggccataggt
     5941 ggtgatgacg gtcttgagca gcgtgcgctc cagcccctgc gcgggacgcc cggcgcggaa
     6001 ggcccggatc gcgtcgtagc catagaggtt ttcggtcgcg ccgtagcgca gggtgtgcgg
     6061 gctgttccag aacagctcgt cgagcacggc cacgtcgttg ccggtcaggg ccttttcgta
     6121 acgctggaac tcggccgtga cttcggccag gacatcggga agattgatgg cgagcatgtt
     6181 ggtttttttc tagagttgaa ccgggcggac atgcgccaca ccggcatcgc tgagagcctt
     6241 ggcggcgcgg aaagccaggt cttcgcgcca cggcgcggca atcagctgca cgccgatcgg
     6301 caggccaccg gtgcctgccg gccacagcgg cgccgtcaca accgggcaac cggcaaacga
     6361 gatgggctgg gtcagcagcc ccatggaagc gcgcgcgggc tgctgcgtgc cgttgatgtc
     6421 cagccactcg gtgccgatga caggcgcgct gaccggcgtg gccgccgtga gcagcacgtc
     6481 ccagtcgcga aacagggcgt tgaccttgtc gcggtagacg cggcgaaaac gctgggcacg
     6541 caggtaccag tcgaccggct gcagggcgcc ggcgatgaac cggtccaccg acagcggctc
     6601 gaagtcatcg gcgcgggtgc gcaggtcgtc caggtgcagg ctgccgccct cgctggcggt
     6661 gatgatgaaa gccgcggcgc gtgccagcgc ggcgtccggc caggtcactg cggcctgcgc
     6721 ccccagggtg ctggcggcca gcgccaccac ctcgcgtgcg gccggcgtgg cgttgtcgtg
     6781 gaaatagccg cccagcacac cgatgcgcaa accctcgaca ccccggccga tcaggggcat
     6841 gaccggctgc acgcgcaggg cctggcagcc cgggtccagg gcgtcgggcc cttgcagggc
     6901 gtcgtagacc aacgccaggc cggccacgct gtcggccagc ggcccgaggt ggtcgatgct
     6961 gtgcacaaac gggtagccgc cacggcgcga gagccgacca aaggtgggct tgaggcccca
     7021 gacgccgcac agcgaggccg gcacgcggat ggatccgttg gtgtccgacc cgagcgacac
     7081 cggcacctgg ccggccgcga ccgccgcacc cgagccgccc gacgacccgc cggcaatccg
     7141 cgtgaggtcg tgcgggttgt ggcaggggcc gtaatgggtg ttctcggtgg tgaagccgta
     7201 ggcgtattcg tccatgttga gaccgcccac cagcacggcg ccggctgcct tcatctgccg
     7261 gaccagaaag gcgtcggctg tcgccgccgc atcgccgcgg ttgaccctgg agccggccag
     7321 ggtcaccacc ccttcgatgt cgaacaggtt tttcacggca taaggcacgc cagccagcgg
     7381 ccccagcggc tgcgacgccg accgctgcgc atccacggca tcggcttcgg cgcgggcacg
     7441 ggcctgtgtc tggtcggtga aggcgttgac ccgaccgtcg gtcgccgcga tgcgcgccag
     7501 gctcgcgtcc agcagttcgc gtgcgctgac ctcgcccgag gcgaccgctg ccgccatggt
     7561 ctccgcgcta tttgctatat ttttcatagc tattgtcgcc cgtcgtcatt gggcggaaag
     7621 gctgccggcc tgtatatttc cgcgagttcg tcgtgcgcag ccaggggcgc ggcctccagc
     7681 agcgccgcca tcacggcggt gcgctgcagg ttcagcgcga cgcgctgggt acgcgccgca
     7741 tccagcggca gcttgagcag cgcggccgtg gtttcgacgt aggcgagcat gcgggtgtcg
     7801 ttcatggggc cctcccgttc aacgccgcac ttcgcgctgg aaaatttcca ggctgcgttt
     7861 ttttgtgccg atgaagctgg cctcggacag cgtttccacc gtgcgcgggc gcgcatcgcc
     7921 atacggcagg atttcggcta cacgggtggg ccgcttggtc agcagcagca cctggtcggc
     7981 caggtagacg gcctcttcga ggtcgtgcga gaccagcagc atggtggtgc cggtctgcat
     8041 gaacacttcc tgcagcttct cgcggatgaa cagcgtcatt tcaaagtcga gcgctgaaaa
     8101 cggctcgtcg agaaacagca cctccgggtt gttcgccagt gcccgcatga tggaagcggt
     8161 ctgctgttgg ccgcccgaga gttcgtaggg gtaacgcttg aggtcgaact tcacgtcgaa
     8221 ggacgcgacc agttcggcca tgcgcctgtc gacctcggcc ttggaccggc cttcgagctt
     8281 gagcgggtag gcgatgttgt cgatggtgcg catccacgga aacatcgctt cgcggtagtt
     8341 ctggaacacg tagccgatct tggtgtcttt cagcgacttg ccgtcgaaca ggatctcgcc
     8401 cgcatcgatg ggcaccagcc cggcaatcat gttgatcagc gttgacttgc cgcagccgtt
     8461 gggcccgaac accgagacga tcttgtgttt cgggatgtcg aggttgaagt cctcgtacag
     8521 cggccagccg gcgaaatact tggtcaggcc gcggatggtg atgtgcgtgc cggccgggcc
     8581 gggctggaag gccggcacgg gaacatcggc aaacaacggg gcattgagaa ctgcagacat
     8641 ggctaccttc cactccagtg aacaatgcgg cgctcgagca ccaggaaaaa gatgttgagc
     8701 gcatacccga gcgcgccggc ggccaggatg gacgcgtaca tgtccttcac gttcatgacc
     8761 tgctgcgaat tgatgatgcg gttgcccagt ccgctgtcgg cgccaatgaa catctcggcc
     8821 acgatcacga tgacgagcgc catggacacg gccgaacgca ggccgacaaa actcggctgc
     8881 aggctctccc agatcagcac gtccttgaac acctgccagc gggtggcccc catcacgcgt
     8941 gccgccatca cgcgttgctt gcgcgcgttg atgacgccgt aggcgctgtt gaacaccaca
     9001 atcagcagcg caccgaaagc cgcaatcgcc accttgttga tgtcggacac accgaagatc
     9061 atgagaaaca gcgggatcag cgccgacgag ggcgtggagc ggaaaaagtc gatcaggaac
     9121 tcaacgctgc ggtaagcctt ctcggagctg cccagcaaca cacccagcgg catgccgaca
     9181 gccgcggcaa tcaggaaggc ctgcaccgtg cgccacaccg tgaccgcaaa atccttgagc
     9241 agcgggccgc cggccaggcc gttgaccagg gcgcccagcg catcgagcgg cggcggtagc
     9301 aggatggcct tgatgaagcc caggcgcacc accagatccc agacgatgaa cagcaccacc
     9361 ggcccgatga agggcagtgc cctgtccagc gcaggcgtgc gttgcgcaac ggccgccggt
     9421 gcgctggccg gcggtgccca gggcgtggca gacgccaccg gcgccgccgt cacggaggag
     9481 gtgccatgct tcatgttcag cccttgtaga gcattgagtc caccatgagg cgcgaactga
     9541 aaatgccttt ttcggagaac agatcgaaga acttctggaa gtgcgcgacg tcggccggtt
     9601 tgaactcgtt gtacatcatg taggcggcca gcggcacctc gccggtcagc gcaccttcga
     9661 tgggggtgta acccttgagg tactggcgcg cctcgaccgg tttgctgcgc acgtaagcca
     9721 cacccttgcc gtaggcggcc acaaacttct ttgcagcatc gggattcttc ttgatgaact
     9781 ccgaggacag cgaggccgag ccgccgaacc acggcgccat gttgtcaccc agcacatatc
     9841 tggagatcac gccggcctcg agcacccgcg tggtgccgtt catgcgtcct atcgtgccgg
     9901 tgggctccag cgtgtaggcg ccgtcaatct ggccggcggc cagcgcggcc acatgctggc
     9961 cgatcggcag ctcgaccacg gtggcaccca cggcgccgcc gcgctccagc acggtcttgg
    10021 ccagggtcac gttctggata ccggggcccg aaccgatgcg tttgcccttg agatcggcca
    10081 tcaccttggc ggggctggcc accggaacga tgacctcgtc gagcacattt ttcacattgc
    10141 tcgggttgga gcagaagatc ttgaaggtac caggctgggc cagctcgccg atggcaatgt
    10201 tggccgagcc ggtgccgttg gcggtgccgt cggaacgtcc ggacagcacg gcctccatca
    10261 cctgctgggc gccggcgaac ttgagcggct ccacgtccag gccggcttcc ttgaagtagc
    10321 ccagttcgat ggccgcataa aacggcaggc cggcggcgat cggccagtag ccgatgcgga
    10381 tcttcggtgc ggcctgggca cgcacgatgg cgggcatgcc cagcacgccc agcatggccg
    10441 cgctggtttg caggactttc cggcgcgagg ccgtgaaggt ggaggaagtg cgggcagtgg
    10501 tcatggaatg ctcctgaagt gaaagaggtt ggtatcgatg aaaggtgtca ggccgcagcc
    10561 gtcttgagtg aagtcatgta ggcgcgccag ccgcccaggt gcgagatgtc cagcgccccg
    10621 tgcagtgctt ctggctcgca gacaaaaccc tgcaccgtgc tgccgtcgcg aagcgccagc
    10681 gtgcctatgc ccagcggcgc ggggatcagc gccacaaacg agccgtaggc agcaagtggc
    10741 atctcccaga cttcaagcgc aatacaggcc ccctggccgg caccgacacg gagcaggccg
    10801 ggcttgggcg gggtggtgcc gggcaaggcg aacagcctgt agtcgggcgc ggtttcggcg
    10861 gggcctgtca gcaccgcccc gcgctcggtc agttgggtat tcaagggcat gcccgacaga
    10921 tgggcgccga ccaccgccac ccgcaccgtc gccattgccg gcttgcgcag gccggcaatg
    10981 gcgcgcatgg cgggcagggg caggccggtc gcgccctgcg tcaggccggt agcgtggtgg
    11041 tagcgctggc caaggtcggc cagttgccag tcgctgccgc aaggggcgat tagcgtgatg
    11101 ccaaacggca gcccgtcggc gcgcacggca ctgggcacgg acagggcggc gtagtcgagc
    11161 aggttcacga aattggtata cgcgccgagg ttacggttga gcacgaccgg gtcccgttgc
    11221 atctgcgcga tcgtgtaatg cgtcggtgcg gtcggcacca cgagcacgtc gatgttggcc
    11281 cccacgctcc ccactgcgtg tggatccggg tactcgctgc ccctttcatg ggtgcagggg
    11341 gctggccttg cctggggcgg tccttcgctg cggccgtcgg cggaacgagc ccacatcgcg
    11401 tcggcctgct ggcccaggcg cttcaattcg gtttgcactt cgtacagatc cgccgcgctg
    11461 tactgtcggc ccgcggcaat gatgctgcgc accggttcga ctacctcggc ttcatgagcg
    11521 tcgaagaagc tgcggatggc ggtgtaacgt tcggccacca gcgcgctttc gtagagcagg
    11581 gccgcgacct gcgccagcgg cgcaaagtcg attgccacgg gcgtaccgcc cagggcctgc
    11641 agccgcgcca ccgcctcggc gaaggccgct tcggcggcac ggtccccgta aaactccagc
    11701 gtggccggca caccaaaacg gaaggccggt gcaaacggtt cgctggccat cgtcagccgg
    11761 cgcgaatacg gatcgagcgc gtcgtgcgcc atggcgcttt ccagcacctg gaccgcggtg
    11821 gccaccgtgc gcgcaaagat ggacacacag tccacgcttt gcgcggccgg caccactccg
    11881 cgcgcgctga tcagcccgcg tgaaggcttg aggccgacga tgttgttgag ccccgctggc
    11941 acccggcccg agccggccgt gtcggtgccc agcgagaagt cggtctgccc ggtggccacc
    12001 acgtaggccg agccggaact ggagccgcct gatacataag cggggttgaa cgcattgggc
    12061 accgccccaa aaggtgaacg cgtgccgttc aagccgcagg caaactggtc gaggttggtc
    12121 ttgccggcca gctgcgcacc agcatcgagc agtttctgca caacaaaggc gctggcagtc
    12181 ggtgtgtagg caaaagccgg gcaacccgcg gtggtgggca cccctccggc atcgatgttg
    12241 tccttggcag caaaggtcag ccccgccagc ggcccggccg aagcagcctg cgcactggcg
    12301 gttgccggcg acttggcgcg gcagatccag gcctgcggcg ccagcaccgg gtcgcccgca
    12361 gcggccagca tgttgtccgg tgccggcgac tggccggtca gatcaggggt taaagaaact
    12421 tgcaccaatt taaagcatcc atacttgtgt acaagatgag atgcaagagg tgtgccaagt
    12481 tccggcggcc ggttcgggtg tggagcgccg gcccaaaacg gaacggattt gctatctaat
    12541 caatagctgt cagcgcctat ccagaaaggg ccgggggcga tttcccccta cgttccccag
    12601 ggcagcccgg caacaaagcg ctgtcagctc tccagcacca gccgcgtcac taccggtggc
    12661 gtctcgcccc gatgaaacgc cagcttgcgg tcgcgcaagg ccacgtcgcc cgccgtcacg
    12721 cggtcaaagc ggcgcaggtg ttcggtccag gattcgtcgg tgatctgctc gacgtaacgc
    12781 cccgggtccg agatgtcgcg cagcagctcc caagccagcg ccccctggcg cagccggctg
    12841 cgccggcttt cctgcatcag cgtgcgaaat tcgagggccc gggccggatc gatgcggtac
    12901 tcgatcatgg tgcgcacctg gccggtcagc ggcggtgctt ctgcactggg caccttgaat
    12961 tcgcgggacg gcgtcaggtc ctcctcgatg ctgcggtcgc gcagcagcaa ctgcgtggca
    13021 cccatggcca ccatcgctgt cgatgcggcg atcagcatgc tggtcgggac gctggtcacg
    13081 gtcgcaatct ggccccagac cgccgcgccc agtgcgctgg ctcccatgat cgccatctgg
    13141 aaaatcgaca tgccgcgcgc acgcacccag tcgggcagcg cgagctgcgc cgacacgctc
    13201 agggtgttgg cggtggtgat ccacgccgcg cccgccagaa acatggctgg cgcggcgacg
    13261 taggcattcg gcgcaaaggc gatgatggcc gtggctgccg actgcaggcc catgccgccc
    13321 agcaccatgg catcgcggga caaggtgcgg cgcagccgcg gcagcaaaaa ggccaccaca
    13381 aaaatcgcgc ccgagcccat ggacgccagc agcagcgtga aggtgccggc tccgccgccc
    13441 tgcaggccac gcgccaccag cggcagcaag gccagcaagg cggtggagtg aaagaaaaac
    13501 agtgccaccc gcagcagcac cgcgcgcagg cgcgccgact ggcgcacgaa ctgcacgccg
    13561 acacgcatgg cgctgatcag gcgctcgcgg cccagcgggc tgggcacatg ctcgcggcgc
    13621 cagcgcatga tcacaaaacc ggacaccagc gaaatcacgg cgttgaggat gaaaacgtac
    13681 tcgctgccgg caccggcaat cagcgccccc gccaccagcg ggccgatgat gcgcgaggca
    13741 ttcatggcga tgccattgag cgccagtgcc gccggcaact gcgcgcgcgg caccagctcc
    13801 ggaacaatcg ccgaaaacac cggccagcgc atggccagcc cgacgccgtt ggcaaaggtc
    13861 agcgccagca gcagcggcgc ggtcatggca cccatcacga tcgtcagaca caacaccagc
    13921 gccacggcgg ccacccagaa ctgcgtgatg atgaagtagc ggcgccggtc caggatgtcg
    13981 gccagcgcgc cgctgggcag gcccagcagg aagaccggca gcgtcgaggc ggactgcacc
    14041 agggccaccc agatcggcga ggtggtcagc gaggtcatga gccaggcggc ggccacgtcg
    14101 ttcatccaca tgcagatgtt ggcggccatc caggtgcccc acaacatgcg aaaaaccggc
    14161 gagctcaggg gtgcccaggc cgacagcgaa gccgggtcgt gacggccagg cgcgggggcg
    14221 ccgggcaggt ccggcgggag ggggctgggc atggggagaa ggtcctgggg caggtctggc
    14281 ttcaatggcc ggcaacgaag ccggttctgc tcaagaaccg tttttgccgg gctgcagttg
    14341 ccaatgcgtt gttacggccc ctgctgcgcc cagatacggc tgatctgcgc agacagctcc
    14401 atcggatcaa acggcttgtg aatcacgtcg atcgctccga gctcgcggta ctgcgccact
    14461 tcggcggcct gcaccttggc cgtcatgaaa atcaccggcg tgccggccgt ggcgggcagc
    14521 gcacgcaggg ccttgagcgt gctcggaccg tccatgcccg gcatcatgac gtccagcagc
    14581 agcaggtcgg cctgggcggt cggtgccgcg gccagcgcct cgctgccgga cgggcaggcg
    14641 atcacggtga aaccgcccac ggcctccagg gccatctgcg ccaccatgcg gatgtcgggt
    14701 tcgtcttcga cataaagaat gcgggcaagt gttttgctca tggctatcgt ccttggtcaa
    14761 gaaagaggtt cgtcggccgg caggctggcc tgcagcgcgg caacggcaag tgcaatatcg
    14821 gtgcgctgga gtggcaaggg tacctgaccg gccggcgcgg cctgttcgag cagcagctcc
    14881 agcgccctgg cggccgcgcc cacctcatga aaccccaggg tgccggccgt gcctgccagc
    14941 gtgtgcacca ggcgctccag ctccgccgga agcgccgtgt cgtcgccgtc ggccaccgca
    15001 ctccacaggg cgtggatgtg cgccattttt tccggcaggg cgcgccggta gtcggcgcgc
    15061 tgctgatcga gaaacctctg gaaagcctgc tgcggggtat ccatacgctc agctttttcg
    15121 cgcccgctcg tacagcggca tcactctggg aagggtgcgt tccatgtcgg caatccgcgt
    15181 ggcgccggcg gggtgggtgc tcagccactg cggcggcgcg cccttgctgg cggcggccat
    15241 tttctgccaa agtgtgatgc cggcgcgcgg gtcaaagccg gcgcgcgcgc caagttccat
    15301 gccgaccaga tcggcctccg actcgtcttc acggccaaaa cgcaggttga tcagctccac
    15361 gccgagacca gcgacggtct ggcccaggtc cccgaggccc agcagctgcg acagcaggct
    15421 ggctccgatg ttggtggccg ccgacttgcc catgcgctcg cgcgcgtgtt cacgcagggc
    15481 atgggccatt tcgtggccca tgatcatggc cacctcatcg tcgttcagct tcagctggtc
    15541 caggatgccg gtgtagaagg caatttttcc gcccggcatg caaaacgcat tgatctgctt
    15601 gctgccaatc aggttgacct cccactgcca gtccttcgca cgcgggttcc aggccacggc
    15661 atgcgggatg acgcgctggg caatgcggcg caggcgctgc agctgcggat ggctggcagg
    15721 ggccaaggcc ttctgggccg ccgcctcctg cagcatcttg ctgtattgct gccgggaggc
    15781 ctgctcgatc tcccccgccg gcaccagctg ggtgaaaccg gagttgccac cgacggccac
    15841 cccttcacgc tgggcgcggg ccggccccgc gcccagcaac aaggccgcgg acaaggccgc
    15901 cagggtcagt ctcaatgcaa atgtcatcgc cttctccaga taaagggcag gttccggcca
    15961 gccggacaaa agagcccgca gcgtattatt cccgcatgag cgagacctta tccgacgatc
    16021 cccaggcaaa acgtcagcca acgccgcttt ctgaagctca actttccccc atctcccccg
    16081 ccccggccgc gcgccccagt tgggccgaga ccttgcgcgt ctacctcgaa gcgcccacct
    16141 tgcgcatgct gctgctcggt ttttccgccg gcctgccgct gctgctggtg ttcggcacgc
    16201 tgagtttctg gctgcgcgaa gccggcattg accgcaccac cattggctac ctgagctggg
    16261 tcgggctggc gtatggcttc aaatgggtct gggcgccgct ggtggaccgc ctgccgattc
    16321 cgctgctgac gcgcctgctg ggacgcaggc gcagctggct gttgctgtcg cagtccgtca
    16381 tcatggcggc cctggtcggc atggcgctga ccgatcccaa aattgccttg ctgccggtgg
    16441 tctggtgcgc gctggcggtg gcctttggct cggcgacgca ggacatcgca ctcgacgcct
    16501 ttcgcatcga gtcggccgac gtgcagcgcc aggccgcgct ggcggcggcc taccagaccg
    16561 gttaccgcat ggcgatgatc tgggccggcg ccggtgtcct gtggattgcc gcgcgggccg
    16621 aggtcgccgg tgcgggcggt gcgaccggct atcagcacgg tgcctggcaa atcgcctatt
    16681 tcgcgatggc ggcgtccatg ctgctgggcg tggtgacggt gctgttttcg cccgagccgg
    16741 cgccgcgcga gctgccgcct gcgcgcaacg ccgccgagtg gatcaaaagc gcgctggtcg
    16801 aaccctttgc cgacttcctg cgccgctacg gcaaacaggc ggcgctgatc ctgatgctga
    16861 ttgccgtcta ccgcatcagc gacgtggtga tgggcatcat ggccaacccg ttttacgtcg
    16921 acatgggtta caccaaggac gaagtcgcgg ccgtcaccaa gatctatggc gtgatcatga
    16981 cgctggtcgg tgcctttgtc ggcggagcgc tggccatgcg ttttggtgtg atgcgcgtgc
    17041 tgatgctggg cgccgtcctg agcgccggca gcaacctgct gtttgcctgg ctgggctcgc
    17101 tcggccacga tgtcaatgcg ctgatcttcg tgatctcggc cgacaacctg tccagcggca
    17161 tcgcttcggc ggccttcatc gcctacctct ccagcctgac caacatcaac tattcggcca
    17221 cgcagtacgc cctgttcagc tccatgatgc tgctgctgcc gaagtttctg gccggttact
    17281 cgggcaccta tgtggacgcg tttggttaca gcagtttttt cacggtgacg gccttgctgg
    17341 gggcgccggt gctgctgctg gtgtggctgg cttcacgcat gaaaatgccg gccaacccat
    17401 gatccacggg agtcgacagc tactgatttc ataggcttca aaagcgccgc aggggccgcc
    17461 ccgatcggcg cgccggttta cctaacttta cagcggcttc aacgcgactt gttgcgcggc
    17521 gcgcaccatg agacggtcat tgacgaagga gtctccatga gtgaccgtac ccccagccct
    17581 ttcaccaccg acgtgttggc ggccacccca ccggcgggcg ccaatggcta cactgatcag
    17641 cccacaagca cccgcacggt gaacccgccc atgacacagc aactcctgat gatcgaagac
    17701 gacacccgcc tggcgaccat ggtgggggaa tacctgcgcc agtcgggtta tggatttacc
    17761 catgcggtcg acggcggcaa gggcctggaa gcgctgcaaa gctgcaagcc cgatctggtg
    17821 atcctcgatc tgatgctgcc cgacatggac ggcctcgaag tgtgccggcg catcaagggc
    17881 ctgggcggcg acgccggccg cacggccgtg ctgatgctga ccgccaaggg cgacccgatg
    17941 gatcgcattg tcggcctgga aatcggcgcc gacgactacc tgcccaaacc ctttgagccg
    18001 cgcgagctgc tggcccgcat ccgtgccgtg ctgcgccgcg gcggcgagac gaccagcacg
    18061 agcggcagcc agcagaccat gcgcttcggc tcgctcgaga tcgaccgcga cgcgcgcacc
    18121 gtgtcggtct ccggcaaggt ctgcgaactg acgtcctacc agttcgacct gctggtcgcc
    18181 ttggccgaac gcgctggccg cgtgctctcc agggaccaga tcatggaagc cgtgcgtggc
    18241 cgcgaactcg aagcttttga ccgttcgatc gacgtccaca tgggccgcat ccgcagcgcc
    18301 atcgaactcg atgcaaagga ccccaaacgc attctcacgg tgcgcggtgt gggttacgtt
    18361 tttgccaagc agcaggattg atccggtctt atgggacttc tagaaattcg attgtcggga
    18421 tgcagggcaa ggcgcacgga gcgcaggaca ccggaatgca cctgggtgca tgaggattcc
    18481 gagcaccgcg caacgcagtc atgcgcccga aaatcggatt tgtaggagtc tcccatgtgg
    18541 tcccgtttta cctcgcacct gtatgtcagg atctggctcg ccgtggtcgc cacggtggtg
    18601 gtgctcgcct ttctcgttgg cgcggcctgg caactgagca cagacccgcc ccaattgccg
    18661 atccgcgaag tcctggtgca caacgctgcc ggcgaagtca tcggtcgggc acagaccagg
    18721 cccgaccgca agctcggtga cggtctggag tttgacgtct tgatcaagga cgggcagacc
    18781 ctgaacctgc agctgccccg ccccaaacgc cctcccggca gttcctggaa ccgtccgccc
    18841 tacggctttg gctttggctg gatgctgggc ctggtcgcgc tggctgtcgc gctgggtgcc
    18901 tatcccatca tccgccggct gacgcagcgg ctcgaaggcc tgcagcgcgg cgtcgagcgc
    18961 tggggcagcg gcgacctgtc ggcccgcatg actgtccagg gcagcgacga agtggctttt
    19021 ctggccgaac gtttcaacca cgccgccgag cgcatcgaaa cgctgatgga gtcacagaaa
    19081 gccttgctgg cctcgcacaa atcgctgctg gccaacgcct cgcacgagtt gcgctcgccg
    19141 ctggcgcgca tccgcatggg cctggaactg atgggtggtg acgccggctc ggcgcagcgc
    19201 gccgaaatct cgcgcagcat caatgagctg gaccagctga tcgacgaaat cctgctggcc
    19261 agccggctcg acaccagaca ggccgacgcc gaaccgttcg aggtgctgga cctgaccggt
    19321 ctggccgccg aggaatgtgc ccgggtgcag gcggaactcg gcacggcgac ccagaccgaa
    19381 agcctcacgc tgcagggctc ggcgcgcctg ctgcgccgcc tgatccgcaa cctgctggaa
    19441 aacgccaggc gttacagcaa cggcgaagtc agcctggaac tcggacagac cggcgcgggt
    19501 gcgcagcggc gtgccgtgat ccgtgtcaac gaccacggac caggtgtgcc gccggaccag
    19561 cgtgaacgca tttttgagcc cttctatcgc ctgccgggcg ccagcgaacg cgaaggcggt
    19621 gtgggcttgg gcctggcgct ggtcaaatcc atcaccgaac gccacggggg cagcgtgcgc
    19681 tgcgaagacc ggccgggcgg cggcgcttct tttgtggtcg agctgccgat gcctgccacc
    19741 gcttaacgtc ctgtcccgca aataaaggcg atatgaaatc gcccattcag ccaccatgcg
    19801 gcgttgctcg tcgttgccat ggctacggct atgtcgcctc ctcgcgcctt gcctggcgac
    19861 tgactggaca atttcatttc atcacctatt tgcgggactg gacactagaa atacgaatac
    19921 gcggcagggc aggcgaaata tacaatcgcc tcctccaccc agggaaggcc ttgtatgtcg
    19981 cccaccgcca ccaccgcgca agtatttttc cctgaacagg gggacggtcc gcaggcgccc
    20041 ttgtccacgc gggtgattgc atgagcgtgc ctctcgcgac cctccagcgc cagtcgaacc
    20101 cggtctggct gcatgacgct ttcagttcgc tgtccggcgg gcatgccgga acacctctcg
    20161 tctttgagcg tgccgaacat gccgccgact acctgcgttt ctggagcgcc gatcccgccg
    20221 ctctcacgtc cctgcgttac gccctgtacc gctgcgaaca ttccccctcg gtgtttgcca
    20281 tgcccgatgc acaggtgctg caagcgctcg ctgccaaact ggcgcgcggc accctggtac
    20341 tgacagaccg ccaggcaagt gcaggcgcag gtggctggcc gctggttccc gccaccgagg
    20401 cgacggtacc ggcctccagt acgctgatca acctcaacac cctgcccgac cggcccccgg
    20461 tgccgccctt gctgcccgtg ctcgaagaag tgcagatcga aggcgccgag gtgctgcccg
    20521 aaatcgaaca gacgctggag cagattgacc tgaccctggg cagcatcgac ctggccggtg
    20581 tgtcgctgga accgacaccg tcgaaagtgc cgctgatcga gaccgccatg accgacgcca
    20641 gcagttcggt gacccggacg ctggacgatc tgtaaagccc gcgcatgcag tttgtgacgc
    20701 gggccggcac ggccatcagc accggcgcca cgcaggtgat cgcgtcggtg gccacggcct
    20761 ccagcacctt gcagaccgaa ctgggcgcgg gcatttccga agccaccgcg gccatcagca
    20821 ccgtgaccca gcaggtcagc gcggtggcca cgcagatcac ccagctgagt ccgcccctca
    20881 atccgcaagc gctggttggg ccgctgtcgc tggcactgac gcgcctgcaa tcggacctgg
    20941 gcggcgtggt gaactcgctc gctggtctgg ggccacgcct ggccacggcg gggcagaatc
    21001 tggccggcat cgtcagcgat gtccaggacg cggtgtcggt cgccacgggt ttgccgggcc
    21061 ggctggtggc gatccgcccc gatgtgtccg gcattccgcc acggttgacc ggcattgccc
    21121 gcagcgcgca ggacctgggc gcccgggcac tgtctctgga accgcgcctg gcgcagtttg
    21181 ccggcacgct cgcgcccctg cagcaacgcc tggccgacct gcagatcagg caacccgcag
    21241 acctcgcgag tgcgcaggcc ggcgtacgcg ccgaggtgca gggcgcgctg gacggcatca
    21301 acggcgagat cggcagcgtc cggctggaag tcgccggcgc tgcgacaggc atccagacag
    21361 aaattgaagc caccgcgcaa agcctggcag cggtgctgga cgggctccag gcccagattg
    21421 acgcgatcgt ggccgacgtg tccggtgtca ttgacacggt catgggccgg atcgatgcag
    21481 ccgtcaccgt gctggaggaa gaactcgccg cactgaccgg acaggtcggc ggtttcaagg
    21541 accgcaccca cgaggtggcg ctggccatcg ccgcgccggt gctgggggtg gaggcggcga
    21601 ttgacgcggt cgctgcgcag attgaagccg tgggccaggt gattgacggc atcctgacca
    21661 gcatcgaggc gcagatcacc cgcctggaaa cggtgatcgc tgcgctcaaa caaaccatgg
    21721 acacggcggt cgccggcgtg gacgacacgc tgaccgtcct gaaggacacg ctgggcgaag
    21781 tcatcaccga gatcgacgaa ctcaaggtca cactgcaagc cctgcccgag cgcttccagc
    21841 cggtcgaggc gcaaatcgac gaagcgcagc agacgattga aagcatccag cagcgcatcc
    21901 gcgagttcat ggaccgtgcc gaccgtgcgc tggcgcaggc cagccgcgag atggaccagg
    21961 ccgacgcgct gtgcgacaac gccatcgccg tctgcaccaa atacatcacg cgctcgctgg
    22021 ccatggtggc ggcgcgcacg ctgttcatgg gcgtcaaggc catgatcccc ggcatcagga
    22081 caaccattgc cggcgcgcgc gccacggtga aaacatcggg caacaccgcc atcggcctgc
    22141 tggaccaggc catcgcggtt gtcgagcagt tgcgggccgt ggtcaaacag gccatcgaac
    22201 aggtgcgcca ggccattgcg caagtgcttg ccgtgctgga ccaggtgcgc gccgccctgg
    22261 tgcaggcgca ggagcgcatg acccaggtct ccaccgacat gcatgcggca gccgagcagg
    22321 tcaaggccca gcccgacaag ctggtggccc aggtacgcga ggcgcgcgac agttttgtcg
    22381 ccgccacgca gccgcgtgag aaagtcaacg ccgtgcatgc acaggtgctg ggtttcaagg
    22441 gccgcttcgt ggcgccggtt cgcctgcagg tggaaagcgc cgagtcacgc gtcatccagg
    22501 gcatcagcga cgtgcaggca aagatgcagc cgcccatgga ccagctcaag gaaaaagtgc
    22561 aggcgctgcg cgaccagatg tcagcgctgc aggcacagat ccggcgcccg cccgcccagc
    22621 ttcaggccct gatggaagat ctcaaggccc agctggacca gcaggtgggc cgcgcgcgcc
    22681 aggtgcttga cgacgtgctc gccaaggccg atggcctgat cggccaggct cagcaggctg
    22741 tgcagtcggc ggcgcaggca gaaagcgacg ccagccaggc cttgtccgca atggccggcc
    22801 gcttcagcca ggccagcagc gaaaccggca gtctggtcac ccggatacaa acggctgccg
    22861 acgatgccac cagccaggtg gacggtctcg gtgccaggct ggacagcttt cgcactgaag
    22921 ccgaagatgc tctccagcaa attcaggcga gtgtgcaaag cgccagcgac agcgccctcg
    22981 ccctgcgtca ggagtttgag gctgcgggcc agtcggccag tcgccagccc gacgccatct
    23041 ctgccagcat ggccgccctg ctcagcgccg cagagcccct gcaaggcgca ctggcctccg
    23101 cgcaacagga cgccgcggcc cagggcgaag cggcgaacca ggccagcgcc gggccgctgg
    23161 aaaagctggc cgccgcccag caggccttgg ccaccatgct ggatgacgca ctggccgcag
    23221 tcgcgccgcc cggcgacgct gcccgggaag aacagcagaa gatggacgag cggctggcgt
    23281 cggtcgcggg tgaagtggat gggctgaaac agcaggtgca ggcagcatcc aaagaggtcg
    23341 ccgagcgcac ggctgaggcg cgcgcggaag tcacccagca tcgcgacaag ggccaggcgc
    23401 tgatcgacga agcgatgaaa gaggtggaaa cggccaaagc gcaaaccgag gtcgcacgcg
    23461 ccgatgtggt cagtgccgag gccacgatgg acgccgaagt ggtcaaacac ggcggcgaag
    23521 cgctggcgcg caaggcgccc cctgcgcaag ctggcgcccc cgcagccagt gcggcgtccc
    23581 ccaccgaagc gggacaagcc gccggcgcat ccggtgccgg cgcgccaggc aacgcaccag
    23641 gcggcggcgc ttcgaccgat ggcgcggcgg cgcccgccgc cgcgccggcc caaggcacac
    23701 cgacccaaca ggaggaacaa ggggccactg caccggacgc cagcgccgcc ccgggcgagc
    23761 ccgcagccga ggcccaggca gcgccgggtt ccgccgcgtc cgcagcaaca gacagcggcg
    23821 ccagcgacac gcctgccgcg cctacggcgg atgttcccgc cgatcagcgc actccggagc
    23881 acgcggcgca tccggcggcg accgacctgc cggacaccga tgccggccca aggcccccga
    23941 ccccttcagc cccccccctg cccggggacg ccgccacacc gtcgcagcca cccgggacag
    24001 cgccacccgg caaaccggca gccgggcccg aaataccgga aaggccgggc ctgcccgagg
    24061 tgcctgaagt gcctgaaaca gcagatctgc caccggtcgc gccgtccggc acgcccgctc
    24121 ctggcggtgc agcacccgcc gacctgccga agcccggcgc gaccatgccc gacccggcac
    24181 cgctgcgccc accccggcct ctgcaagacg cgctggacaa ggccacctcc gcgaaaccgg
    24241 ccacgccggt cccggaggcc ctcaagtccg gcctgaacga cctggccagc aagctgccca
    24301 caccggcgga cgcccccttg gctggaactg atgcagaaaa gcttttggag ggcctcaaag
    24361 aggctctcac cggccccccc gggctctgag gaaaccgccg tgatggcgca ggcgtgaaga
    24421 aaatctcaat ttgaaatact gttacgcgaa aaaacgacat ctctccccca aataggggat
    24481 gccacaaaaa ctatctaccc tttaaagtct cgcagttgct gtgacaacat cgatcgctga
    24541 aaacaaaggc agaagactcc agatcgatca acagatcatc agtctcaacg acgtccctca
    24601 aaggacttgg caaggccacc gcaaggtggc ctttttttgc ccggttacat ctgcgggaca
    24661 gccgctctcc ccgcgttacc atcggcagct acttgattgc gggagtttgc gcgtgaacac
    24721 caaccaaccg gcggcacctg tccgccaggg cttctccact tctgccccgg aacggcttcc
    24781 catgctgccg gccgacgcga tgtccgccgc gcagcgcgaa gcggccgacg ccctgatcgc
    24841 cggcccgcgc aaagcggtgt tcggtccttt cattcccctg ctgcgcagtc cggcactgat
    24901 ggcgcgcgtc ggccaggtgg gcgagtacct gcgttttgac agcatgctgg acgtgcgcat
    24961 ccgcgagttg gcgatgtgcc tggtcgcgcg ccatgtgtcc aaccagtttg aatggctgat
    25021 gcatgcaccg ctggcgctca aggccggcgt gccgcaggcc agcctggagg ccatcgccga
    25081 aggcagatac ccgcacagca cagcccccga cgaagcagcc atggtggact tcgtgaccga
    25141 actgctgcag caccatggcg tcagcgacag ttcgtacgcc gccgccctgg cgctcttcgg
    25201 cgagcagggc gtggtggaac tgaccacgct ggtcggctat ttcgtgatgg tgtgctgggt
    25261 gatgaatgtg gctcgcacgc cgggccaacc ccagccgggc ttcagcgccc tggcggcctt
    25321 tccggcctga ggaagcgctg aataagtcct tgcggaggcg cggcagccgg atcgggatgg
    25381 gatg
//
LOCUS       DAIDFG010000007        23998 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_7_length_24022_cov_20.245129, whole genome shotgun sequence.
ACCESSION   DAIDFG010000007 DAIDFG010000000
VERSION     DAIDFG010000007.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 23998)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23998)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23998
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7_length_24022_cov_20.245129"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         4168 a         8051 c         7536 g         4243 t
ORIGIN      
        1 tgccgggccg caggcgcagc gccccctcgg ggcagcgagc tacacgcagt gagcgagcgt
       61 ggggactgta ctcagacccg ttcctcgtcc tttttctgct tgatcatctt ggtcttgatg
      121 cggttgcgct tgagcggcga caggtactcg acgaacacct tgcccatcag gtggtccatc
      181 tcgtgctgga cacacaccgc cagcaggcct tcggcgctgt gggtctgcag ctggccctgc
      241 aggtccagcg cccgcacctt gatggcgctg gcgcgctcca ccccgtcata cacgccgggc
      301 actgacaggc agccttcgtc gttgacctgg gtctccgggc tggaccagat gatttcgggg
      361 ttgatcagta ccagcggctg gtcgcggccc tcgctgacgt cgatcacaac gactctttcg
      421 tgaacattga cctggctggc ggccaggccg atcccctcgg cggcatacat ggttttcagc
      481 atgtccccca ccaaagtgcg gatacgcgcg tccaccgccg ccacgggctt ggcaacggtg
      541 tgcagacgcg ggtcagggta acgaaggatg gtcagttggg tcatggtggc aggcaatcag
      601 atgtcgctat tttcgctaat tttcgccatt tttcagcatc cccgctgtcg cgaaatggca
      661 tattcgttgc ctaatcaagg gcttaagtac aaaatcgcta gtgatttatc aagatacaaa
      721 cgcggcgtcc ggccttgatg cccaaggccg cgccagcccc aagggcctca agatgcaaca
      781 tatttttggt cggttcagtc agatttcaat agcggccgct ctgctggcat ccaccgcagg
      841 tctgcacgca cagaatttcc cgatcaccgc cggccagaaa gccacggcca gccaggtggc
      901 ccaaaccggc gtgccgctga gcgatctggc ccccaatgcc cccgacgaat acaccgtcaa
      961 gcgcggcgac accttgtggg ccatctcggg catgttcctg aaaaccccgt ggcgctggcc
     1021 cgagttgtgg ggcatgaatc tggatgacat ccgcaatccg caccggattt atcccggcca
     1081 gcagctgtat ctggacaaat ccggcggccg cgccactttg cgggcccggc gcgcgggcga
     1141 cggcgacggc cagcccggca ccgtgcgggt atcgccgcgc acgcgttacg aatcgctggc
     1201 cgacgcagcc atcccgacgt tgtcgccaca ggccattgag ccctttctgt cggaagccct
     1261 gatcgttgat gaagccgcgt ttttgaccgc cccgcgcatt gtggccgccc aggaaggccg
     1321 tgtcttgctc agccgaggcg accgcgccta cgcacgcggc gtttactcaa acaaggacac
     1381 cggcaagccg ctgagcacag ccaaaggtga gccgctggac tatcgggtct tccgcaatgc
     1441 gacaccgctg aaagacccca ccaccggcga aattctcggc tacgaagcac agtttcttgg
     1501 caaggccgag ctggtgcgcg gcgagtccac cgccagcgtg aagggcaagg acggcaagct
     1561 cgtcgatgag atcatcccgg ccaccatcga catcctcgtc gcgaaggaag aaatgcgcgt
     1621 cggtgaccgg ctggtgccgg aaccgccgcg cgaactgctc agttatgtac cgcgcgcgcc
     1681 acagctcccc attgagggcc agattgtctc gatttatggc agtgccgtgc gttatgcggc
     1741 gggcaaccag gttgttgtga tcaaccgcgg catccgggac ggtctggagc ggggccatgt
     1801 catggcaatt ttgcgaagcg gggaacggat caccgaccgc acggactctg ccagaacaca
     1861 gatcaagctg cccaacgaac gcaacggctt gttgattgtc ttccgcactt ttgaaaagct
     1921 ctcctatgcg ctggtcctgg aggtaagcga aggcgtaaaa gtgggcgacc acttcgaaaa
     1981 tcccaattaa gcatcagcgc cagcttgtgc agaccgtctg catgaattgc gttgccgcct
     2041 ccgcctgaat ccgacccttc catggacgtt gctgaactcg gcgcctggtt gcgcctgtcg
     2101 ctgaccccgg gtatcggcaa cgcgggagcc cgccagctgc tggccgcgtt cgggtcagcc
     2161 caggccatct ttgaacagga cgcaagcacc ttgcggcagc tcggctccga ccgcctggcc
     2221 gatgcgctac tgaccgagcc cgccaccctg gccgcccaac tgcaaaccac cctggactgg
     2281 ttgcagggcg ccgacaaccg gcgtgtggtg acgctgggcg atgccggcta cccgcccgcg
     2341 ctgctcgaca tcgaagaccc accttccatg ctttatatgc ttggatccct cgatccctgg
     2401 gcgccggcag ctatcaatag cgcagctccc tgcctcgcta tcgtgggcag ccggaacccg
     2461 acgccgcagg gggaaagcaa tgcccggcaa tttgcctggg tgttcggtga agccggtctc
     2521 tgtgtggtgt cggggctggc gctcggcgtg gacggcgcag ctcacgacgg cgccctgctc
     2581 ggcggcggca ccaccctggc cgtggtcggc accgggctgg accgggtgta tccgaagcag
     2641 cacctggcgc tggctcaccg cattgctgaa aatggcctgg tcatcagcga attcccgctg
     2701 ggcacaccgc ccctcaccgc caacttcccg aagcgcaacc gcctgatctc cggtctgtcc
     2761 caaggcacgc tggtcgtcga agcggcgctc aagtcgggct cgctcatcac cgcgcgcctg
     2821 gccgcggaac agggcaagga ggtgtttgcc atccccggct ccatccactc cccccagtcg
     2881 cggggctgcc acgccctcat caaacagggc gccaagctgg tcgagtccgc ccaggacgtg
     2941 ctggaagagt tgaacctgcc tcagctttcg actgccttga ccgcagaacc ggatgcggac
     3001 ggacagcctg cagacgaccc gctgtttgcg gcgatggggt acgagcccgt cagcctcgac
     3061 gccctgcagg cgcgcaccgg gttggcaacc gccgaattac aggcccgtct gctggcgctg
     3121 gagatggccg gggaggttgc caggctgcag ggcgggctat ttcagcgcat ggcgaggggt
     3181 tgacgcctac ggttacgtcg ggtcaagggc cttcggcggc gtacaacggt cacaccgttc
     3241 ccaactgcgt tatattgagt gcatgtttga agtactggtg tacgtttacg aaaattactg
     3301 gcaaggcgac gcctgccccg agttgcacca gctgagccgc aaactcacgg cggtcggttt
     3361 tgaggccgac gagatccagg cggcgctggt ctggctcaac ggcctgaaca tcgccgcgca
     3421 gaacacccag gttggtatgc ctgccgcatc accggccagc gccccccctg tccccgcaac
     3481 ggccaccaca ccgccgggct tccagcccca gtcggccggc agcctgcgtg tttattcagt
     3541 ggcagagcag gaccacctcg gcgcccaggc cctgggcttc gtcagtttcc tggagtcttc
     3601 gggtgtgctg ccatcccaca tgcgggaaat cgtgatcgac cgggcaatgg ccgcaccggg
     3661 agaccccctt gcgctggacg acctcaagat cattgtgctg atggtgtact ggagctttgg
     3721 cgaagagccc gatgcactgg tcctggacga actctgcgac gacgcggact tccgcgtggc
     3781 gcattagagc ccccacgttc gctcactgcg tgtagctcgc tgccccccga gggggcgctg
     3841 cgcctgcggg ccggcacagc cggacccgcg gcccctgctg gtaaagaagg ctcctcaccg
     3901 cgctggcgat cagcccagca ggcgctccgg attggcatcg agcttggcca gcgcatcgcg
     3961 ggtggcgcgc acggtcaggg cttcgtcttc ggtcggcacc agcagaccgg cctgcaggta
     4021 ggccgccaga cggcgcgcct ggatcaggaa gttgcggccg tcggtcgatg caaacaggtt
     4081 caactgcttg cggtcactgc gccaggcaaa ctgcacatgg ctgacctggc cgttgtggtc
     4141 cagcgtgaac caggacccga gctgcaactc ctgcgcccag gaacgcatgg cctcgctggg
     4201 ctggctgcca ccgtcggtaa tcacctcgat ttccgaggca tcaatgccga tcatcatgac
     4261 gaggctttcg gtgtccagcg gcaggtcgcc gacatcctcg tccgacaggt agtcctccag
     4321 attcgccagc cgcctggaca tttcctcaat gcgctccagg gagatggcat cggtcttgga
     4381 catgaaggcg tccgccagcg tatcgctgat gatcttcagg tgctggtcct gcaccggcgc
     4441 ggtcatgccc agcatggaca tgcccagacg cagcaggtgc agcagcttgg gcaggtcggc
     4501 aatgacgcgg gcccggtcat tccggttggg cttggcgctg gcggcccaga cgagatcggc
     4561 cgccgcctgc ttgagcgtga tcgtgtcctt gtgctggggc ccgtttttca tggctgcaat
     4621 ggccagcacc tcggcccaga tcttgaaaag gaattcacgg atctcttcgc gcacaggcat
     4681 gtcgttgagc atgttgcgca tttcgatggt gtactggatg gccatggttt ccttctgttc
     4741 catctgctgg gccacgctga ccacgcgcgc ggcgctgcct tgtccggaca ggaatttcga
     4801 aaggaatttc tggaactcgt catacaccag ctggaacacc cgcctgccgg tctccgggta
     4861 ttgctcgatg acctgcacga tgcgcttgat ttcaatctcc agcgcaccgc ccgagatcgt
     4921 cgcatcaaaa ccaagcacgc aggagcccat gcggtcgatc agccggcgtg ccggatgctg
     4981 cagggaaccg aaaaattccg gttctgaaat cgcaacacgc agcaccggca tctgcagcct
     5041 ggcaaaccac acccgcacgg caggcggaat gcggtcttcc gccagaatgc tctggaacat
     5101 cagcgccacg atttcgatgg tggccttttc actggatgtg ctcgccgcct gcttgaggtc
     5161 gctggtgcgc tggcgcaagg cgcctgccgc ctgctccaca tcgacggcac cataaacctg
     5221 accgggtgca tcgatcaagg tgccttcgta ggcattgccc gtcgcggcgc gggtttgcac
     5281 cctggacagg gcctgcgcca gctggggtga gggctgattg gcgtgggtgt gatcaaaatc
     5341 aacggcgtgg tcggacagca ggcgcttgag atgccccatc accccctgcg cccgcatgcg
     5401 cgcccgtgcc agtggcgacg tcccggtctg catgcgtgtc tcgtcctgga tcgcttccct
     5461 ggctgcggcg gagtttgcgg ccccccccgt agcacccgac gagctgccac cgccaccgct
     5521 acctccacgc cctgaacctg aacctgaacc tgaacctgaa cgtccgccgc ccccggcccc
     5581 aacgcccgaa gagccccctt caacgccagc ttcaccggac gcctgcccga ccggcgccgt
     5641 ggcgcgacgg ccctccggct tgggtttggc gcgcacgggt gacgggctgt ttgcagaggg
     5701 cgtgcgtttg acgagtgccc gcagatcgat gtccaccatc acgttttcct tgatcaggaa
     5761 ctcgttggcg gcgcggtagg cctccagcag atgctcggtc aactgctgct ggatcaggtc
     5821 ctgcaccgtc agccacacct cgcgcgacag gggagccagg gtccattgct ccaccatctt
     5881 ttgcgagagg acttcgggtc ggagaatgtc ttccttggcg agctcggaaa ctccctcaag
     5941 gctctggacg cgcagacgca ggtcgttgag ctcccaactg gcactgtcca gcaggcgcaa
     6001 agccaggcgg gaggcaagaa tcctgttctc catcacttcg ttgcccatca gctcgaactt
     6061 gccggtatcc accgccatgc cagctggcgc ggccgccggg agcagtgcat ttttccaggc
     6121 agcgatggtg gcgttgaccc agaccgcttc cgaatcctga aaggacaacc aggcgtcacg
     6181 gcgatcctgc atgtcgcggg cactgccgct ctgctcggtc agggtcgaca gtttttgccg
     6241 gatggcgcca gccagctcgg gcaggatgcg cgcggcgtgg ctcacaaacc gttcgcgcgc
     6301 ctgccgggca agaagaaggt ctcttttggt gtttgcggcc acgtccgtat agatgttggt
     6361 gcggtttgcc taaaccgttg ccccggcatt cgggtcgttg gggtcctctt cctttttccc
     6421 cgatggcttg acaaggtctt cgcgcttgat gcccagccac atcgcaatcg ctgcggccac
     6481 aaacaccgaa gaatagatgc caaacaggat gccaatcgtc agggccagcg caaagtaaaa
     6541 cagggtcggt ccgccaaaga gcagcatcga caacaccatg atctgggtgg aaccatgcgt
     6601 gatgatggtg cggctgatgg tggaggtgat cgcgttgtcg atgatctgca cggtgttcat
     6661 cttgcggtaa cggcggaaat tttcacgtat ccggtcgaaa atcaccaccg attcgttgac
     6721 cgagtagccc agcacggcca gcacggcggc cagcacggag agcgaaaact cccactggaa
     6781 aaatgcgaag aaaccaagaa tgatcacgac gtcgtgcagg ttggcaatga tggccgccac
     6841 cgcgtacttc cattcgaagc gaaacgccag gtaaatcatg atgccgacca ccaccatggc
     6901 cagcgccttc aggccatcct gggccagctc ttcgcccacc tgggggccga caaactcggt
     6961 ccgccgcagg gcgatctgcg ggtcatccgc cttcagcgcc gccatcactt tctcgctctg
     7021 ctgggcggtg gtcacgcctt tttgtgcagg cagccggatc atcacttcac gcgccgtgcc
     7081 gaaattctgc acctgcacat cggtaaagcc caggcccgcc acggtacccc tgaccttttc
     7141 gacatcggcg ggctgcgaat agctgacctc catcaccgtg ccgccggtga actccaccga
     7201 cagatgcagg ccacgcgaaa acaggaaaac cacggccagc acaaaggtcg tgaatgaaat
     7261 cacattgaag atcaaggcat gccgcatgaa cgggatgtct ttgtggattt tgaagaattc
     7321 catggcgttt tctgtccgtt gttctgtggt ctagttggcc gacgctgcag gcttggcagg
     7381 cacagcagtg tttccggcgg gtcgccagac ggtaccaatg gacaccgatt tgagtttctt
     7441 ctgccgtccg taccagaggt tgaccagccc gcgcgagaag aacacggccg agaacatgct
     7501 tgtcaaaatg ccgatgacgt gcaccacggc gaatccgcgc acgggtccgg aaccaaaggc
     7561 cagcaaggcc agcccggcaa tcagcgtggt gatgttcgag tccagaatgg tggcccaggc
     7621 gcgctcatag ccgctatgga tggcggcctg cggtgccacg ccattgcgca actcctcgcg
     7681 cacccgctcg ttgatcagga cattggagtc aatcgccatg ccgagcgcca gcgccatggc
     7741 cgccatgccg ggcagtgtca gggtcgcctg cagcatggac agcaccgcca ccagcaggag
     7801 caggttcacc gccagagcca gaccggagaa cagcccgaac agcatgtagt aaacggccat
     7861 gaaagcgacg atcacgagaa agccccaggc cacgctgtga aagcccttgg cgatgttttc
     7921 agcgcccagg ctggggccga tggtgcgttc ttcaatgatt tccatggggg cagccagcga
     7981 tccggcacgc agcagcaatg ccaggtcatt ggcttccgtc acgctcatgg agccggaaat
     8041 ctggaagcgg ttaccgagct cactctggat caccggggcc gtcagcacct cgcccttgcc
     8101 tttttcaaac agcacgatcg ccatgcgctt cttgacgttt tcgcggctca cgtcgcgcat
     8161 gatgcgtccg cccttggcgt ccacggtcag gtccaccttg ggctgctggc tctgcgagtc
     8221 aaaccccggc tgggcatccg tcaggctttc gccggtcagg atcacctgct tcttgacaat
     8281 cacaggtcgg ccgtcgcgtt ccaggaagcg ctccgagcca aaagggacgg cactggcgcc
     8341 gctttcggcc gcgcggcctt cggcgctttc atcgaccagc cgcatttcca gcgtggccgt
     8401 gcgccccaga atatccttgg ccttggccgt gtcctgcaca ccgggcaact gcaccacgat
     8461 gcggtccaga ccctgctgct ggatcaccgg ctcggccaca ccgagttcgt tgatgcggtt
     8521 gtgcagcgtc accatgttct gcttgagtgc ctgctcctgc accagacggg ccgcctcggg
     8581 cttgatgctg gcattgagct tgtactcggt tccgtcgggc gcgtccgcca gttgcaggtc
     8641 ggcaaactgg tcctggatca ggcgtttggc ggcgtccagc gtggccgtgt cacggaacct
     8701 gacctcaatg acctggccgt tgcggttgat gccgccgtgg cggatgttct tttcccgcaa
     8761 caccaggcgg atgtctccgg ccagcgactc ggcctttttc gtcagtgccg cctgcatgtc
     8821 cacctgcagc atgaaatgca caccgccgcg caagtccagg ccgaggtaca tgggccgggc
     8881 gttcagggcg gtcagccagg cgggtgagcg tgacagcaga ttgagggcca cgatgtaaga
     8941 cggtgtggcc ggatcgggca gcagcgcttt ttcaatcgcg tccttggcct tgagctgggt
     9001 gtccgtgtcg ccaaaacgtg ccttgaccga cgtgacatcg aaagtcaccg cctccgcaac
     9061 gatattgaca tccttgagcg ccttctcgac gcgagcgact gttgtctcgt cgatcctgac
     9121 gctggacctg gcagcggaaa cctgcaccgc aggcgcctcg ccaaaaaagt tgggcagcgt
     9181 gtaaagggcg gcaatcaaca gggcgacgac gatgatcgcg tacttccaga ccggataacg
     9241 attcatgagc gtgctgttcc tgggaaactg aggggctgaa aaagccggtc catgccggcc
     9301 ggccgggaga aacgactagg acttgatgct gcctttgggc aggacctgga cgatggcact
     9361 gcgctgcagc tggacctcga cgcccgtggc aagctccagg ctcaggtagg cctcgcccag
     9421 cttggtgacc gtgccgagca ggccaccggc ggtgaccact tcgtcgcctt tggccagtgc
     9481 gtcaatcatc gcgcgatgct ctttctgttt tttcatctgc gggcggatca tcacgaaata
     9541 cagcaccaca aacatcagca ccagcggcag catgctggtg agcgaagaca acatgccgcc
     9601 ttcggcagca gcggctgggg cggtttgggc aaacgcggaa gaaataaaca cggcagactc
     9661 cacaacaata agtaaggctc ccactggtca cagcgggggc gcggaaggcc gattgtatgc
     9721 gggcgggtgg atgtcaggac ttgtaaccct accggttttc gccctttgca ggcccttacc
     9781 gtccattgtc ggccggcaaa tgcggccagc cgagcttttt caggccggac gcgacctcca
     9841 gccccgcccg ccgggtcctc aggcaaccag ttggcgctcg ccggcgaccg gctggcggaa
     9901 tgccgccgtc gccgcgctcc atggttgctg ggcagcctcg acatggcgcg ccttgacgtg
     9961 gccatacccc ttgatgagct ccggaatacg ggccaggtca agggcagcgg catggttgtc
    10021 cgcatccagc gtgcgcagca cttcctcaat gctggcctcg taccgggcaa tcagtccacg
    10081 ctccatccgg cgttcttcgg tgcgtccgaa cgggtccagc gccgtgccgc gcaagccctt
    10141 gagcctggcc agcagcttga agcccgtcag catccagggg ccgaatttct gcttttgcag
    10201 ctcgcccctg tcgttttgct tcgcaatcat gggcggggcc aggtgatagt tgagcttgaa
    10261 gtcgccctca aacatcgcgt cgaccttgtc caggaaactg cggtcggtgt gcagacgcgc
    10321 gacttcgtat tcgtccttgt aggccatcag cttgaacagg tacttcgcaa ccgcttgcgt
    10381 gagcagcgtt tttccgagtg ccgattccgc ctgctggacg cggttcacga aactttcgta
    10441 gcgctttgca tacgccgcat tctggtaatc cgtcagaaag gccacgcggc gcgccaccag
    10501 ctccggcagg ccctggcgcg gcgccggcat gctgatgacc tgcgcggagg caaacagctt
    10561 ttccaccgag ggcaggtcgt gggccgcgcg gcggccccat tcgaaagccg tcttgttgtt
    10621 gtccaccgcg accgcattga gctcgatggc acgcatcagc gacgcgtatt ccagcgggat
    10681 ccaccccttt tgccaggcgt agcccagcat catcgggttg gtgtaaatgc tgtcacccat
    10741 cagcttgatg gacgctgcgt cggcattgaa ggcaccgaca ccggccagcc ccacggcttt
    10801 ggcgatctcc gcggcgcagg cctcggccgg gttctgccag ttcgcgttgt gcacaaacgc
    10861 cgccgtcggc gtgctgtggg aattgagcgc cacgtgggtg cggccctcgc gcatgcgcag
    10921 caccgtttcc ttgccggccg tcacaatcgg gtcgcagccg atgatcaggt cggcgccggc
    10981 catgccgaca cgcgtggtgc ggatatcgtc ctggctcgcg cctatcagca catggctcca
    11041 ggtggcgccg cctttctgtg ccaggccacc ggcgtcctgc gtgacgatgc ccttgccttc
    11101 gatgtgcccg gccatgccca gcaactgccc gatggtgatg acgccggtgc cgccgacgcc
    11161 ggccaccacg atgccccaga cagcgccgtt gacaccttgt gtcgcgggga tctgcggctg
    11221 cggcagggcg tccatctcaa acggtgaagc cgccttgccc ttggccttct tcttgagttg
    11281 tccgccctcg acggtcacga aactcgggca aaagcccttc aggcaggaca tgtccttgtt
    11341 gcaggtggac tggttgatct ggcgcttgcg gccgaactcg gtctccagcg gttcgacgct
    11401 caggcagttg gactgcacgc tgcagtcacc gcagccttca cagaccagct cgttgatgac
    11461 cacgcgcacc gccgggtcga ccatggtgcc gcgcttgcgg cgccggcgct tttcggtggc
    11521 acaggtctgg tcgtaaatga tgaccgtcgt gcccttgatc tcgcggaact gccgctggat
    11581 ggtgtcgagc tcgtcgcggt gatgcaccgt cacgccttcg gccagtgcca caccgtcgta
    11641 tttttccggc tcgtcggtga ccaccacaat tttggccgcc ccttcggccc gcatggactg
    11701 ggcaatctgc accaccgaca ggccttccgg ccgctcgccg atctgctggc cgccggtcat
    11761 ggccaccgcg tcgttgtaga gaatcttgta ggtgatgttc acgcccgagg caatcgactg
    11821 gcgcaccgcc agcgagccac tgtggaagta ggtgccgtca ccgaggttgg caaagatatg
    11881 ctggtcggtc gtgaacggct gctggccgac ccagggcacg ccctcgccgc ccatctgggt
    11941 gaaaccggac gtgctgcggt ccatccacaa tgccatgaaa tggcaaccga tgccggccat
    12001 ggcgcgcgag ccttcgggca ccttggtgct ggtgttgtgc ggacagccgg agcaaaacca
    12061 gggctggcgg tcggccttga cttccagcac ctgcagcgag cgttccttgg cttcgaggat
    12121 ggccagctgc gcgtcgatgc gcgccgtcat gtcgctgtcc aggcccagct tgcgcacgcg
    12181 ctgggcaatc gcgcgggcga tcagcgaagg tgacaggtca gcgttggcgc gcaacagggt
    12241 gttggccgtc gggttcggca tggaccattc gccgccgctg aagtcaccct cgacctcgtt
    12301 gaacttgccg tagatgttgg gccgcacgtc ggcgcgccag ttgtagagct cttccttgag
    12361 ctggtactcg atgacctggc gtttttcctc gaccaccagg atttcctgca ggccggtggc
    12421 aaattcgcgc gtgccctgcg cttccagcgg ccagaccaca cccaccttgt gcaggcggat
    12481 gccaagctgg cggcaggtgg catcgtccag cccgaggtcg atcagggcct ggcgcgtgtc
    12541 gttgtaggcc ttgccgctgg cgatgatgcc aaagcggtcg ttcgggcctt cgatgacgtt
    12601 gtaattaagc cggttggcgc ggatgtaggc cagcgcggca taccacttgt agtcgaacag
    12661 gcgcgcttcc tggtcgagcg ccgcatccgg ccagcggatg tggacaccgc ccggcggcat
    12721 ctggaaatca agcgggatct tgatctgcac gcgttcaggg tcgatcatcg ccgtggcact
    12781 ggactcgaca atttcctgga tggtcttcat gccggcccag acgcccgaga agcggctcat
    12841 ggcaaacgcg tgcacgccca ggtccagaat ttcctgcaca ttggtcggga aaaacaccgg
    12901 caagccgcag gccttgaaaa tatggtcgct ctggtgcgcg gcggtggagc tcttggccac
    12961 atggtcgtcg ccggccaccg caatcacgcc gccccaaggg gtggtgcccg ccatgttggc
    13021 gtgtttgaac acgtccgagc agcgatccac gcccggcccc ttgccgtacc agatgccgaa
    13081 cacgccgtcg tacttgttgg tgcccggtgg cgaaaagccc agctgctgcg tgccccacag
    13141 cgcggtggcg gcgagctctt cattgacgcc cggctggaac acgatgttct gcgctttcag
    13201 gtagctggac gctttccaca gcgcctggtc gtaaccaccg agcggggaac cgcgataccc
    13261 gctgatgaag ccggcggtgt ttttgccttg cagggcgtcg cgctggcgct gcagcatggg
    13321 cagcttgacg agggcctgca cgccgctcat gaaggcctga ccgtaatcga gcgagtattt
    13381 gtcgtccaag gtcaccgttt ccagggcctt gcggatgtgt tcgggcaggg gtgcgttcat
    13441 cttgtctcca gtcgtggggc tgacaccgcc ttttgggcag cgcgaagagg ctcacacccg
    13501 gatagggtga tgcctctata taggagcaaa gtgtaggcgc gatggccaga taagtctttg
    13561 ctttttatgc cttgtttaac tcgccttaag caagattctt gcttaaaatt gccaattatg
    13621 gaaacactcg acaagtttga cctcgcgatc ctgaatgaat tgcagaccga cgggcgcttg
    13681 agtaacgccg aactcagcac ccgcgtcggc ctgagcgccg ccccctgctg gcgacgcgtc
    13741 agggccctgg aggaaggtgg cttcatcaag ggttaccgcg cggagatcga ccgccacaag
    13801 atcggcctgg gtgtgctggc ttttgtgcgc ctggacgccg accgcaacac cggcgatgcg
    13861 accagcgcga tggaagaggc gatccgcaaa ttgcctgaga tcatcgcctg tcactacatc
    13921 agcggcaccg gaactttcga gttgcaggtg gtggcccgcg acctcgacag cttctcgcgc
    13981 ttcgccctga aaagcctgct gaacctgccc aatgtgaagg acatgcacac cagcttttcc
    14041 ctgggcgaga tcaagtccag cagcgtgctg ccgctgggcc acttgtcaga caagacacca
    14101 tgacagcccg cgccatgcct gtttgccgct cacacgccgt ttcggccctg tttgcggtgc
    14161 tttttatggc tccagccctt gttgcacggg cgcaatcagc tcctgatttg atagtaaacc
    14221 aggcgccctt gccgacgcgc aatctgcaaa ttgaggtacg ccagctgcgg gacagcagcg
    14281 cgtcccggaa caccttgggc gccggcggca cggtgcgcat gcagcccggc cattctggag
    14341 gcgatgtcca catcgacgcg caaagcggcc agcgaaccgg gtcgggcgac gtgacccagc
    14401 gcttgctggt attgaacggc cgcagcgcca acatcctgct gggcaacagc gtccctgtgc
    14461 gactgctgca cagctttgtg caaaacggaa tcgtgcgtta cggcagcgcc acggtgctga
    14521 tgaacgccgg cagcggtttt gcggccaggc cgttctggcg cggcggcgac agcgccgagc
    14581 tggaactggc cgccgtgtca agcccacgca gcgcgacttc gccacccacc gattcccgcg
    14641 tggtgacaac gctggttgcg ccgctcaacg agtgggtgac cgtggcccag tcggaggagg
    14701 acagcaacga cagcagtgcc ggcttgttca acaacgcgca gtcgacaacc cgctcgacgc
    14761 tgacggtgca gctgcggatt tcggtacgtt aagggacttc ttttccgtcc cgcacgcagc
    14821 gcgccaccag cggcagcagc gggtaacgca gcaccgcctc acgctcgccc gaacccaggc
    14881 caaactcggc gcgcatacgc gtgttgctgt acacggtctg cgaggcgcct tgcccgcctg
    14941 cgtcacgaaa aagcacgtcg gaggcgcgcg aactctcgat cagcgcactg tcatccacga
    15001 acctcacgct gaaatcaatg ccatgttcac agcggtaacg cgttgccact gatccggtgc
    15061 cgggggcggc gcaagcagag agcagggcag ccgcagcaag cagcaggccg cagcggggga
    15121 gaaaggtggg cggcaggctc atttgggcca caacacccac aagcgcaggg gctggttcac
    15181 gcggctggcc agctcgcccg cttgcggccc cgtcccggca aaatagtcgg cccgcacggc
    15241 accgacgatg gcgctgcccg tgtcctgcgc gatcacgagt ttttgcaggt ccgccgccgc
    15301 gccgcgcgag gcgagccaga ccggggtgcc gtaaggaatg ctttcccgat ccacggcgat
    15361 cgaacggccg ggcgtcaggg gcacgccctg ggcgccgcgc gggccgaagg ccgcgtccat
    15421 ctcgctcagg gtttcttcgc ggaaaaagat ataacgcggg ttgctccaca gcagttgcga
    15481 cacgcgctgc ggattctgcg ccgcccaggc cttggtggat tcggtccagg gcgcgttcat
    15541 ccggccctgg ccctgctcca tcagccactg ggtgatgctg cgaaaaggct ggtcgttgga
    15601 accggcaaac gccacccgca cggtacgctg cgcacccccg gcatcggtga tggccagccg
    15661 gcccgagccc tggatatgca gcgacatcgc atcaatcgga tcggccagcc aggcgatttc
    15721 ccgcccgcgc agtgcggcct gcgcctcggg cagcgtctcg atttcctgcc gggtgtacca
    15781 gggcttgcgg ctgcccaggc cggccggagg ctggtacagc ggcaccgggt aagcggcgcc
    15841 gggctgccgg ctggccctga gcacgggttc gtaatagctg gtgagcagtc cctcggccgt
    15901 gccttgcagg gtttcgacgc ggtaaggctg cagccggccc accatccagg cgcgctgctc
    15961 ctcccctgtc gcaatgctca ggcgccttac ctcgccacac agcggtgcaa acaccggccc
    16021 gggccgctca cagcttttga gccaggcgtt ccaggcttcg aacagcgcgt cattttcaaa
    16081 ccccggcagg tcgctccagg ccaccggcac ccagcggctc ttgccgcgca ccatggtgcc
    16141 agcctctgcg cctgacccgg gacgcgcagg cacgctggca gcggcgggcg ccgaaggccg
    16201 ggaagcaggc acgggcgccg tcgcgcagga caccaggccc agcacggcag cgataatgac
    16261 aagcgtcggt gttatccggg aaaaaacagt catggcctta cttttactcg aagccctggg
    16321 ggccctgctc attctggttt tcattgtctg gtggacgatg ttctccggcc gcaacaaagg
    16381 cgaactcaaa agcgatgccg atgcccagcg cgatgcacca ccggaaaagc ccggcgatcc
    16441 gctttagcgc tataaattac ggagcagatt cgccagctcc accgccgact ttacctgcat
    16501 tttgtcaaaa acccgggccc ggtgcacttc caccgtgcgc acgctgatgt cgagctggtc
    16561 ggccaccagc ttgttcggca ggccctccac caccagcttc atgacgtcac gctcacgctc
    16621 cgtcaggcct tccaggcgca catgcaggct ggtgtgcttg ctgcgatcgg ccagcgccgc
    16681 tgccgactgc atcaacgcct gctcgatgcg gtccaccagc acattgtcgg aaaagggctt
    16741 ctcgcaaaaa tcgaaagcgc cgcgcttgac ggcatcgacc gcggtcggca cgtcggcgtg
    16801 accggtcagg aaaatcaccg gcagggtggg cagcaggcca tacccaatca gcttttcgaa
    16861 catggccagc ccgctcatgc cgcccatgcg cacatcgagc agggcacacg agggcgagga
    16921 aatggcggac gcccccgaga tccgttcgtc gaacgcctcg gcgctggcaa acgtctcgct
    16981 ggtcaggcgc cgcgaacgca gcaaccaggc cagcgcctcg cgcacgctgg catcgtcatc
    17041 gacgatgtag accgtggcat tctggatggg ttccatggca attccttccc gcaagcgggt
    17101 gttcaggtgg acacgtcggt gaccgcgacg gcggtgaccg cggccggcaa tgtaaaggtg
    17161 aaaatcgtac cctgtggctg gcccgccgca aaaccaagaa acccgccgtg ctgctcgatc
    17221 accgtgcggc acaggctcag acccaggccc atgccttcgg ccttggtcgt gaagaagggc
    17281 gtgaacagct ggcgcgccac gtcctccgaa atgccttccc cgtggtcgat cacggcaaac
    17341 tccacccatc ggctgtgtgc gttggaggcc gccggccgca cacgcaggat cagcagcttg
    17401 tcggcgcgcc ggcttttgtc cgtcgactgc atggcctgca tgccgttgcg cgccaggttg
    17461 agcagcactt gctcgaccat ggtccggtcg cacagcaccg gctcgcagct ttcgtccacc
    17521 tggatcagca cccggacccc cagcttgcgg gcctgcaggc tgatcagcgg tatcaccgcg
    17581 tccagcaggg cgcgcggcgc gaccggctcg cgcacctggt cgcgcctgcg cacaaagtcg
    17641 tgcacgctct tgatgacctt gccggcgcgt tcggcctgct cggcaatgcg gcgcatcgcc
    17701 agctgaaggt cctggtggtc gggcggctgc gcttcgccgg ccaccgcgct ttgcagcaga
    17761 ttcagcgagc cggtcgcgta gctggaaatg gcggccagcg gctggttcag ttcgtggctc
    17821 agcagcgaag ccatctcgcc gaccgtggcc agcctggccg tcgcctgcag ccggtcctgg
    17881 ctggcgcgcg acaactcctc gacgcgccgc tgttcgctga catcgagaat ggcgctcatc
    17941 cagccggtct gcttgcccac ggcgttgatc agtggcgcct cgatgatcag caccggaaaa
    18001 cgcgagccat cccggcgcat gaagacggac tcgaagcctt cccgcggcag ggtcgcgccg
    18061 acggccagtc gccgcgcctg ccgctgctcg tactcctcga cctgctcggg cggccagtaa
    18121 ggcggcggcg tgccgtgctt gagcagctcg ccggcctcca gccccaccat ctggcaaaag
    18181 gccgggttca cgtaggtcac gcgaccctgc aggtccctgg cccgcatgcc ggtgaccagc
    18241 gaatcctcca tggccttgcg aaaggccagc gcgtcggcca ggtcatgctc gaccttcagg
    18301 cgccggcgca tgtcgcgccc gagcagcgcc agcacggcga gcagcgccag cgacatggct
    18361 gccaccaacg cggtcagcag gttgggaaac agggccgggg ttccgagctg gcgctccagc
    18421 cggagaacca tggtgttgcc cggcagatcg agcagctgct gcgcgacata gacgcgattg
    18481 ccgcgggccg ggttgccgta catggcaagg cgcgtgccgt cggcctcggt gaacgccagg
    18541 ccgtgaccac gcgccagttg ccggcccacc agctcggaca ggatctcctg cagcgaatag
    18601 gtcgccagca ggtagcccgc cagttgcccg cccgccagca ccggcaggca catgtccagc
    18661 acctccagcc ccagcccgtc ggcctgcggc aggaagtagc tgctcgaata ggccgggccg
    18721 ctgaaacggc gcgccgccgc gcaggcaaaa ctgacatcgg tctgcgcgct ggcgcgccca
    18781 agccgctcaa acaccggggg ccggtaaggc gtttccgcat aagagaccgg cgccagcgcc
    18841 acatcacgcc actccagccg caacatttcc cggtgctcgt gcagcagcgc ggacgcgggc
    18901 acacgccagg cggccggatc cgggctgctg gcctgcaagg cctgaatgcc ctggacgtta
    18961 cgcgccagtg cagcgcgaat gtcggtggtt gtctgcaccg cgtcctgctc cagccggctt
    19021 tgcacctggc tggactcgta gcgcccggcg agccagacca gcgtggacaa caagccgagc
    19081 aggagcgcca ccagcagggc ccacaagccc cagcgccggc tccagcgccg ccggtccgaa
    19141 aacggttcgg cgcggctggc cgcttcgctg ggttcgctgg gctcgctcac aacaccccgt
    19201 ggtccagcgc gggcagctgc aggcgaaccg cccgcaacct gtccgcgctc acctcggcca
    19261 tgaccaccgc ggggccctgg gcctgctcgc ccagcacttc tccccaggga tcgaccacca
    19321 ggctctggcc ccaggtgcgg cgcccattgt cgtgttcgcc cccctgggcc gcggccacca
    19381 cataggccag gttttcaatc gcgcgcgccc gcagcaggac ttcccagtgc gcctggccgg
    19441 tggtccgcgt gaaggcgctg ggcaccagca gcaggtccgc cttcatggcc cggtacagct
    19501 cgggaaaacg caggtcgtaa cagacgctca gaccgacccg gtaacggtgg ccatcgaccg
    19561 agggcagctc gaaggtcact ggcgctgcgc cgctttcaat cacacgcgac tcgtcgtact
    19621 gctccagccc gttgtcaaac cggaacaggt gaatcttgtc gtaacgcgcg acgcactgcc
    19681 ccgtcggcgc atacaccagc gagctgttgc gcacatgctg acggtcgctg gcgccgagcg
    19741 gtacggtgcc gcccacaatc cagagcccca gctcgcgcgc agagcgggcc agaaaatcct
    19801 ggatcgggcc cgcgccagcg gcctcctgca cggcgagctt gtcggtgtcc ttcagcccca
    19861 tcacgcagaa atactcgggt agcactgcca gctcggcgcc ctgcgcggca gcacgctcga
    19921 gcaactgcct ggccatgacc aggttggcgg ggacgctggt ccccgagacc atttgaaggg
    19981 cggcgacgtg catggctact tttttgctcc ggtgtcctcg gcgggcttct caccgggctt
    20041 gacctcggcc gcggccttgc gatccacctt ggtgattttc gggttggtcc aggtgccatc
    20101 aatgtggaat tcctgggtcg ccgcctcggt cagcgggcgc cgcaggaaat actgggccag
    20161 gaaggagccc aggccaatcg ccggattgat gacggtggca atcagggacg cggtgccggc
    20221 gttgatctcc ggcaccacca ccaccgtcag gtcctgggtt tctttggcga tgtcggcttt
    20281 gccttccatc agcacggcgg cgttgacgcc tttcatctgc aggttgttgg taaacgccag
    20341 gccctggttg atgctgacgt cgccgcgcac gaagtcaaag gaaaatccgt ccgaaaacac
    20401 gtcgcggaaa tccagcgtca ggcgccgcgg cagggcctgc aggctgagca ccccgagcag
    20461 cttggcaatg cccggttcgg ccttgaggaa ttggccggag gccacattca cattgaactg
    20521 cccgctcagg ctcgggtagt ccagactcag cggcgagccc acccaggcga tctggccttc
    20581 aagcttgccc ttgccgcgac ggatcacatc gctcatgccg aaacgcttga gcagttcgcc
    20641 cgaatcggca atgtcgagcc tgaaattcat cacggtacgg cgccgctcga ccgccccgcg
    20701 cgacccgccc gcagccaccg gctgctgcgc attgacggcc acccagttgc cggtggcggt
    20761 cagcgtggcc tccggcacga tgacattgag cttgttgagc cgccactcgc gcgccccccc
    20821 ttcgcgggcc accgtcccgg cgccgcggtt gatggcttca atttcaaccc ggcccagttt
    20881 cttgccacgc agctccaggt cctccaccac gatgtcgagc gccggaacgt tggcaggctg
    20941 ctcgttgagg atggcctcga cctcgctggc cgtgctctgg cccaggctca ggcgcgacag
    21001 gcgcgcatac agccggccgg cacccgcgcc cccgggctgg cgaaactcga cgtagccgtt
    21061 gagttcggtc gcgtcgatgt tggctcgcca ggtcaggccg tcacgcgatc cgccgagcac
    21121 gacgttgttg agttttcgcc catcgaacac cagctccctg gcacgtatcg ccatggtggt
    21181 cggcagataa ccgagcacgg cactggcggg ggtcagccgc tcggccggtg ccgcggcaac
    21241 cggcgccggg ctggcggcca gcacttgctc ccaggcatcc atgtcaacac tcgcgagatt
    21301 gatgttggcg gccaccccgg cctcaggcac cggcgcactt tcaccaggct ccaggccaat
    21361 ggcaatgctg ccgcgcaaca cacgcggctc cggcccgctg atgtcgcgca cgtactgcac
    21421 cgaggccagc cgccccacgg tcagggacag ctggtcctgc agggcctgcc ctgcggtcgc
    21481 gccgcgcacg accgtgttgt caaaacgcac cggcaggacg gtctcggcac tcttgttcag
    21541 cggagccggc aggctcagcg ccatgccctg caggttgctg gacaaactga tctcggtggc
    21601 gccgcgccgg aacccgaggt tggcggtgta ggcagcagtg ccgctggcgc ggctgcccag
    21661 gcgcgccacc agatcgagcc cccgggcctg ccgcagcccc tcggcgctca gggtgccctg
    21721 ggcccgaatg agcacggccg catccggaaa ctcagcgggc cgggccacgc cggcctgcgt
    21781 tgcgcgcgtg cccccctcca ggcgcacgtc gccgcccagc aggcgcgcct gcccgccgct
    21841 gatggcaaag ccgctttcgg tgaagctcac cagggccttc gcacgggtga gctgcggcgt
    21901 gtccggcgtg aactgcacat cgttgcccgc caggctgaca ctgcccttca ctttggattt
    21961 ctcgaggtcg cccagcggca ggctcagttc aagcttgtag ccggccgtcc cggtcgccac
    22021 ggtggtggcc agcgccttgc cggtgatgtc gcccaggggc gaggtattga cgacggccac
    22081 ggcctcggcc agcggccctt tgatctcccc caccacctgc accgtggccc caagcatgag
    22141 gtcgggaata cgggcgtcgg ccttgaccac ctgcagaccg ggaaaaccgg cgatccgccc
    22201 cgtcgcgcca ttgacctgca tggaagcacg gtcgaacacc agttcgccgt cgagctcgct
    22261 cagggcgggc caggcactgg cgtcacgcgg ctgcaccgac cgcggcacat aggccagggt
    22321 ggccttggcc accttggccg acacccggaa ctcgccgagc ttcggatcgg caaacggcat
    22381 gtcattcaaa ttgcccttga gccggaattt cacgtcgctg agcgcgcctt gggtcactgc
    22441 atcacgcaca tagtggcgca ccgtgtcggg caggatcagg ggcaggtagc gatgcacccg
    22501 gccgccgtcg ccgcggctca gactgccctg caaatccagc acaccgggaa agcgaccgtc
    22561 atgggcggcg gcgccggcag tcgcagtcgc taccggtgcg tctgcggtgt gccagctggc
    22621 ctgcgcgctg ccctgggcat cggcgttggc aaacaccagg ttgcgcagct gcaattcgat
    22681 tttcgggccg gtcagcttcc attgggcgtc ggtcgacagc tggtcgaacg gcaggcgcgg
    22741 gtcttcgaaa atgccgggca aatacaggtc gccattggtg atcctgactt tggcctgccc
    22801 gccctgctgg gtcaggtcaa aatccactgc cgcaccccgc accccgggcc ggtcgctggt
    22861 cccctgggcc gcggcctggg gagcaatttc aagctgcgtc acccgcccct tggcagcgta
    22921 ggcaagaggc gcatcgagcg cgccctgcca gcgcgcttca acaacatcca ccaggcccct
    22981 gggcgtcagt gaagtcagca tggcatgcgc cgcatcgccc agcggcagtc ggctggcgat
    23041 catcgccagc gcggccagat cgagtttgtc cgcgcggaac tcgccgcgcg cagctgcctt
    23101 gccctcggca ccggtgtagg ccagcgacac attgccgccc ggccactgca ggccatcgcg
    23161 ggtgcgaaac tgcagcccct cggtggcgaa ctcgaagccg ttggcaaact gccggccgcc
    23221 aaaacggccg ctcacggcct gcagcgccag cggctgcagg gccttgccca gttgcgcatc
    23281 caccgccagc agcgccacat cggccgtgcc gccggtgacc tgcccgtcgc tgacatcggc
    23341 ccagacccgc agcgcgccgc tgcccgcccg caggtcgatg cccaggctgg ccaggttggc
    23401 gtaacgcttg acctcggaca catcgacccg cccgaactcg gcatacatct gcccggccca
    23461 gcggcgccac tggccggcgc cgcccgacca cagcggctca cgaaacacac cgcgcaggct
    23521 gaaacgctcg ccccattcgg gcggcggcgt cgcatccagg cgcatcaggt ggcggcgccc
    23581 cgggttgcgc aatgtccagt ccacctgggt cagggccagc agcgggacct gccgcaggtc
    23641 gtcgctccag cgcaccgtgc cgccgcgaat gacgaactcg gtttgcgaaa aaacccagtc
    23701 ggcgatggcg ctgctgtcgt cgttggggtc gcgcgacaca tccaggccgg ccacataaat
    23761 cttgccgtcg gacgtgcgcc ggatatccag ctcgggctgg tcgatgtaaa gctgttcaaa
    23821 acccagcccc cacagcgagg ccggcgagac ggccgccagc acgcgcggca ggcgcagggc
    23881 ttcgcgcccc tgggcatcca gcagcgccac atcgcgcagc tcgaaggacg gaatcagccc
    23941 gcctggccgg gccgtgatct gaccgatgcg caccggtaca cccagggcct ggctggct
//
LOCUS       DAIDFG010000008        23919 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_8_length_23919_cov_17.844494, whole genome shotgun sequence.
ACCESSION   DAIDFG010000008 DAIDFG010000000
VERSION     DAIDFG010000008.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 23919)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23919)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23919
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8_length_23919_cov_17.844494"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3935 a         7556 c         8111 g         4317 t
ORIGIN      
        1 ctgtttgtat ttttcaatgc gctgctcgac ggcgtccttg atggccgagg cgggcagcgg
       61 ccggcgctcg gtgcacaggc gcaggatgag ctgcgggccg acgctttcgg ccagcaccgt
      121 gctcttgttg ccgcgcgggg gcacccagcc gctggattcg gcctgggtgg cgccgcaggg
      181 catgaatcgg gtcgcctgaa gcgcttcttc cagcgcatca aggggcggca gggtgaaatc
      241 gtcggcaatg cggaaaaaag aagcagcttt gaacatgaaa ttccggtgtg gggtgtcaga
      301 gggagtgggc ggcccgcagg ccggtcggca atttttgcgg ggcaagcgcc tggctgagga
      361 aattttaggt gacgctcagg ctgaatgcgg cacttgctta ccttgagatc gggcgccgga
      421 agctccctgg cgaaagctca tgcgatatcg atcacatgga tgccgtacgc gccctggcgc
      481 cggtctgcaa aataacaggc cagcgtctct ttcacggttc tgaaggccag gtcgtcccac
      541 ggaatttcgg cttcggtgaa cagcctggcc tcgatcgttt cgtgtccggg atggaactgg
      601 tcgctgagca gccgcgcgcg gtagtagaga tggacctgac cgacgcgttc cacattcatc
      661 acgctgaaaa gttcttccat ttcaaactgc gcgccggctt cctcgtcggt ttcacgtgca
      721 gcgccctggg acgtggtttc gttgagctcc atgaagcccg ctggaagtgt ccatttgccc
      781 cagcgcggtt cgatgttgcg tttgcacagc agcaccctgt cgccccagtg cggcacggtg
      841 ccgaccacat tgagcgggtt ttcgtagtgg atggtgccgc aagccggaca gacggcgcgc
      901 tcccgggtgt caccgtcatc cggtatgcgg taagtcaccg cggcgccgca gtttctgcaa
      961 tgtttgatgg ggctacggta catgccagcc agtttagttt gaaatcaaaa agggctcccg
     1021 agggagccct ttgttccaac tgcccgaggt caggctttgg tggttttggt gtgtttctcg
     1081 atcaggctgc gggccgtgtc cagcaatttc ttgccgtcga agccgcgctt gttcatgtct
     1141 tcgacccact ctacttccac ggtgcgtgac ttgcggcgga actcctgggc ttctgccggg
     1201 ctgacggtgt agatcgtgtt gccgcggtcg ctggcggtct tgcggcccgg gacgtcgttg
     1261 ccctgctgga ccttgcccag ccaggcggag gtggccatgc cggagttgtt gtcgatgact
     1321 ttcttcaggt ctggcgccag tgagttgtac ttggccttgt tcatggccat cacgaaggtg
     1381 gtggtgtaca ggctgcctcc agccggatcg aactcggcat ggaatttggt gagctcgttg
     1441 accttgaccg agggcacgac ttcccacggg atcacgcagc cattgatggt gcctttgctc
     1501 agcgcatcgg tgatggccgg caggggcatg ccgaccggga tggcgcccag catgcccatt
     1561 agcttggtga cctggcgggt aggcgcgcgc attttgaggc cgcgcatgtc ggcgatggac
     1621 cggacgggtt tgtccacggt gtgaatcacc ccggggccgt gcacatgcag tgccagaacc
     1681 tgggtgtcct tgaactcgtc cggagccatg gtctgcacgt attcccaata ggccttggat
     1741 gtggcttcgg cattggacat catgaacgga agctcgaaag cctcgatgcg gggaaagcgg
     1801 ccggcggtgt tgcctggcag ggtccacacc acgtccacga cgccgtcctt ggcctggtcg
     1861 taaagctgca ccggcgtacc gcccagttgc atggcgggat aggcctcgaa cttgatacgg
     1921 ccacccgatt ccttctcgac cttcgccatc cagggcttgt gcatgcccag ccagacattg
     1981 gaggccgggg acatgaaggt gtggaacttc agggtgactg cctgctgggc caggcccgac
     2041 aggctgggtg cgcccagcgc agccgctgcg ccggttttga gaagggtacg tcgctgaatc
     2101 atgtgttgtc tccggtaagg ttggttgtaa attaaggcag gaagaagcgt ttgtgaaccc
     2161 gtgattgccc tgatggggcc gctagctgat gtactttaca agccacagcg agatgccggg
     2221 gaagaacagc agcaacgcgg tgcgcagggc atcgctgatc aggaagggca cgacgccgcg
     2281 gtaactctcg ctgatgggca cgtccttggc catgctgttg acaacgtaga cgttcaggcc
     2341 caccggcggc gcgagcagtc caaaaccgac ggtcatcagc accatgatgc cgaaccagat
     2401 ggcgacggac tccctgggca tgccgaaatc gagcgccatg accatgggga agaagatcgg
     2461 aatggtcagc agcagcatgg acagctcgtc catgacagcg cccagcacca cgtaaaacag
     2521 caggatggcg gtgaccacca tcagcggctg caggccccag ctgccgacca cttcagccag
     2581 ctggttgggc acctgggtca gcgcgagcgc cgagttcatg aggtcggcgc ctatgaaaat
     2641 caggaaaatc atggccgaac tctccgccgt ggcataaaag cagcggagga acttggccca
     2701 ggtcagttcc cgcttgaaca gtgccgcgac gaaggtggct gccgcgccga ccgctgcgcc
     2761 ttcggtcggt gtgaagtagc cgccataaat acccccgaac accagcagga aaatgacgat
     2821 gatggggaag acggccaatg cagcgcgcag agagatccct tcgctgtcgg catccacatc
     2881 cggcgcctgg cccgggacca ggcgcacata gatggcgatg gcggtgatgt agcccagcat
     2941 ggcaatcagg cccggcacca tggctgctgc aaacagcttg gcaatgttct gctccgtcag
     3001 gatggcgtag atcaccagcg ggaccgaggg cggaatcagg atgcccagtg tcccgccggc
     3061 ggccagggtt gccgtggaca ggcgccccga ataaccgtgg cgtttcatct cgggcagagc
     3121 cacgctggtg atggtggccg cggtggctac cgaggagcca cagatggcgc caaaggccgc
     3181 gctcgccagc accgaggcca tggccagccc gcctttgaag tgacgcatga tgctggcggc
     3241 aaactggaac agcgccttgc tgatgccgcc ctgggtcgcg aaatggccca tcaggatgaa
     3301 cagcggaatc accgacaggt cgtaactggc aaagcgggca aaggcctggg tattgagaaa
     3361 gctcgagaaa ggcgcccagc cggtttgcat cacgtagccg atgccgcctg cggcaaacat
     3421 ggcgatcgaa atcgggaccc gcaacgccat caggcccaac atgatgccga agatgaggag
     3481 agtcagcgtg atggcgttca tgcgtgcgct cccacatcac caccgaggcc gaagacgctc
     3541 tggtagagcc cgatcaccga ggtcaggaca aaagccggca ccatggccac atagacggtc
     3601 cacaccggaa aacccaggat ctgcgtctcg gaatgggtct cgtaggagtt caggccgccg
     3661 agtgtggtgc gccaggccag cactgcgaag accagggcca gcaacaaggc gccgaggcgg
     3721 tccagcctgg cgttggtttc gtggctgagc ccggtggtga agaagtccac gatgatgttg
     3781 ccgcgctgga gctggcagta aggcatgaag agagcaatgg cggccccggt ggcgacgccg
     3841 gtcagctcga aatcgccgac cagcgtggcg ccggtggtgt tgcggcctat caggctgatg
     3901 caggtcatca gggtgatgcc ggtcagcaac aggcccgcca ggatggcgca aagctttgca
     3961 aggttctgca ggattttcaa acggggatct cccagggatc gggcgggtgg ggacggaagg
     4021 cgcaccggaa ggccggcgcc tcatggtgaa gattatccgg ggcaccggac cgaggggtca
     4081 ggcgggatac ccccaaggcg ccgggtattt gcgcgatgat cggagcgctt gcgcgaaaag
     4141 cgcgcaagcg cccggagcgg tatcaggcca ccttgacggt caatgtgccc agccccttga
     4201 cttcgccgac catggtgtcg cccggcacca cggccgccac accctcgggc gttcccgtgt
     4261 agatcaggtc accgggctgc agctcccagg cggcggacag gtgttcaatg gtctcggcaa
     4321 tgttccagat gagcttggac acattgctgc gctggcggtc cttgccgccc acctgcacgt
     4381 acagctcggc atgggcgacg tcgccggcct gggcggccgg cgtgatcggt ccgatcggtg
     4441 cggaatggtc ataggccttg ccgatgcacc aggggcgccc ctgcttcttc atttcgccct
     4501 gcaggtcgcg gcgtgtcatg tccaggccca cggcataacc gaagatgtgc ttgtgcgcat
     4561 cggcggcctt gatgttcttg ccgcccgtgc cgatggccac cacgagttcg atctcatggt
     4621 gcaggttgct ggtcaggctg gggtagggaa tggtgccggt ctggccggct tccaccgcca
     4681 ccaccgcgtc ggcaggcttc aggaagaaaa acggcggctc acggccggta aaccccattt
     4741 ccttggcatg ctcttcgtag ttgcggccca cgcaatagat gcgatgtacc ggaaagcgtt
     4801 cggtggtgcc gacgacgggg acggacacta ccggtggcgg ggtaaataca aagctcatga
     4861 caacctctct tctctgtgta tgcccagtgc ctgatgcacc gggcggtctg aaaaactgaa
     4921 caacacacag ccctgcgccg agcgcagccg ggcggtgtgc cacgacggga tcacgaaatg
     4981 gtcgcgcgga ccgaactcaa aggactgctc ctggccggca gggccaatca gcaccgagcc
     5041 ctggccttcg acaacgctga acaccgcgcc gtcggtttgc cgccagggca ggccggaaaa
     5101 accggccggc aggcgctgca tgaaggtgcc catggtcggc atcggtgagc cgccggtcgc
     5161 cgggttcacg taacgcagct tcacgccgtc ccaggcatca atcggtgcct gttgttccag
     5221 cgtgtgcagt gcctcgcgcg tacgttcata aggatagctg aagatcggcg aagtctggcc
     5281 gaacggggcg tcgtaatgca ccggcagcat gttggcgccg taacgctggt aactggtgcc
     5341 ttcggcccgg gtgaccgtct gcacgcgctc ggtatggttc tcggcaaagc cggcgtcgaa
     5401 aaaacgcagc atgggaatgt ccaggccatc gagccagacc accggctcgc cgccctccag
     5461 cgcacactgg ttgccatggt cgtgccaggt ccagctgggt gtgatgatga agtcaccggg
     5521 cgccatggtg gtgcgttcgc cgtccaccgc ggtgtaggcg ccgcgtcctt ccacgacaaa
     5581 acgcagcgca ctctgggtat ggcggtggct gggcgcaatt tcgccgggca ggatcagctg
     5641 caagccggca tacagcgact gcgtgatgca cgactggccc ggcagcgcgg ggttttccag
     5701 gatcagcacg cggcgcacgg cttcttcggc ggtgatcagt tggccggact gcatcagcca
     5761 gggatggacc ttgtcccagg cccagaaggc cggcacgcag ggcgtggccg gctgctgggg
     5821 caccagcgca tgcagcacct cccagagggg agtcatgttc tgtgcgcgta tcgcctcata
     5881 aaaggtctgg cgtgtattgc cggcgggggg cgtcatgtcc atggtctgct tccatttccc
     5941 ggtttcagcg gtttcagcgg tcaaaataaa caccgtcgtc cagcagcacc tgctgcaggc
     6001 gggcgacatc ggtggcggca aaggccgccc catcagccag ctgtgcggcg gcggtgccgg
     6061 ccgcctggcc catcacaaaa cagccaccgc tggcgcgcgc tgccgactgg ccttcgtggg
     6121 ccatggacgc acagcggccg gcgaccagca ggttgtccac ctcgcgcgtg accagcatgc
     6181 gccagggcag gtcgttgtag gcccggtttt cgtcgcgcgg aaagccccag ctgatggcgc
     6241 cgtccacatg catttccatg ggccaggcgt tgatgccgat cacgtcggca aaacgtgcag
     6301 aggacaggat gtcctcgccg gtcagcatat aagccccgca gatgcggcgc gtctcgcgga
     6361 tgccgacctg gggcgcgatg tcgatgatgg ccgagtctgc aaacccgggt acctcgtttt
     6421 tgagaaagcg gaaatattcg gtgatctgcc gccgcccttc gagttcgccc tcgctgagct
     6481 ggcgcgcgtc ggtggcatcc atcgcattgc cggccgcgtt gcggatctgc gtgacattgg
     6541 cgcgccattc tgcgggattc ttgttgggac gcaggatcgc gcctttgcgc ggaaagtgat
     6601 aacgcccggg ctcgcgcaac tggctctcgt ccatgagctt gttgatggca ctgaactcgc
     6661 ctaccgcagc cagagcgcgt ggggcgtcga cgtggccgac acgaaacatg gtggaaggaa
     6721 acagggcgct gccgtggccg tcgccgagtt cgaaaggcac gcccgcaaag tgcgccacat
     6781 cggcatcccc tgagcaatcg atgaagcggc gcgcccggat cgcctgtcgg ccggatttgg
     6841 tttcgaccac cagggccgtg atggtttggc cgtccatcag gactgcggcc gcccacgcgt
     6901 gaaacaggag ctgcacaccg ctggccacca gcatctggtc ggccgcgcac ttgtaggccg
     6961 agacatcgta ggagcgcaca cggatgcggc cggccatgcc gtcctgcggc tggttcagtc
     7021 cgccgagcgc gtccaggcgt gccagcaggt cgtccaccac gccgtgcacg acctgccgca
     7081 tctcgccgtt tttgcggcca tacagccctg caaaattggt gacgccgccg gctgttccca
     7141 tgccgccgag aaaaccgtaa cgctcgacca gcagtgtgcg tgccccatgt ttcgcggcac
     7201 tgacgctggc cgccaggccg gccggcccgc cgccgatcac gacgacatcg aattcgccgt
     7261 acaccggcag cgtacgtgcc ggctcgttaa cggtgttcat attgggcgaa gcgggcatga
     7321 tgacctgata gggagatagc gacgcgcaat gagcgaccgc gggtgctctc catccaagcc
     7381 ggggccgcgg gtccggcttc gccggcccgc aggcgcagcg gcccctttcc cagtgcaagg
     7441 ggcagggagc cgccgcaggc tcgcagtgag caaccgtggt ggctcattca atcttgatcc
     7501 cgcgcatgct gatgatgccg cccatgatgg cggtttcact gcggatgcgg ctgctcaggc
     7561 cttcaggcga ggttcccacc gcctgccagc cctggttgaa cagcttctgg cgtgtttcat
     7621 tgtcgcgaat gatgcggggc acttcctgcg ccagccgcgt ctgtgcggcc tttgacagcc
     7681 tggccgggcc gaccagcgcg gtccagactt caaggttgag gttgcgcacg tcggcctcgg
     7741 acagggaggg gatttcgctt gccagcgcac tgcggccgct ggtcagtccg atggccttga
     7801 gtttgccggc tttgacctgc ggcagcgcga tgccgggcgg caccagcgcc atctggatct
     7861 ggccaccgag cagcgcggtg atcacctgcg ggttgccctg gtagggaaca tgcacggcag
     7921 ccaggttgcc agccttggtc ttgagcagtt ccatgcccag atggccgacc gaaccgttgc
     7981 cgaccgagcc atagttccat ttgtcaccgc cgttgcgggc ggccaggaag aagtcgttgc
     8041 cggacggctg gttcaccggt gccaccagta ccagcggcgc ggtggtcagc agcgagatgg
     8101 gggtgaagtc cttggccggg tcatagggca gcctggaata cagcatcttg gccgacgtca
     8161 ggttgccgtt gatcactacc gccagggtgt ggtcgtcggt ggctttggca gccaggtcag
     8221 cggcaatgtt gcccgaggcg ccgggacggt tttccacgat gactggttgg cccagtgcgc
     8281 gcgacagcgg ttcggccagc gtacgcgcgg ccatgtccgg ggtggagcca ccggggaaac
     8341 ccaccagaag cttgacgggc cgcgttggcc agttggtggt gacaggcgcc gcctccctgg
     8401 ctgccggctt cttgggcgca gattgagctg tagccagcat gggtattgcc aatgccgcta
     8461 tcaataaggt agctgatctg aaaatgttca tgcaaatctc catttatgaa agcctgttat
     8521 tggaacagtt ttgatggctc gattccgtca tacatccact tcagtcctgc aaatccagcg
     8581 ctttcgttgc cactttgaaa cagctggttg cgcagttcgc gttgcacgcc ggcggcatgg
     8641 taaatgtcgc cataaaaacg cgcggtcaac tgcactcttc cggtgcgcag gtagcgggcc
     8701 tgctggtatt tctggaacgc cagcggcaca tcgccgcgcg caaagcgcag ggcttcgcgt
     8761 agcaccaccg cgtcttcgat cgcctgcccc gcaccctggg ccaggtactg cagcatcggg
     8821 tgggccgcgt cgccaagcag cgtcacgcgc cggtcggtcc agttcttcac cggctcgcgg
     8881 tcgcacagca cccacatttt ccaggtctcg atcttgccca gcagttcccg aacctgcgga
     8941 caggcgtcgc ggaaacgttc cgtgagctcc gccgggtcgc cgaaggtgtt ccagccctcg
     9001 tcgtacttgc tgctgtggaa caccgcgacc aagttgaaca gttcgccgcg gcgcagcggg
     9061 tagtgcacga ggtgggtttt ttcgccgccc cagagcaaca cgtcgtcgct ccacaaatgg
     9121 gcggggacgt cctcgcgctt gagcaccgcg cggtaggcaa tgtggcctga cacgcggggc
     9181 ttgccgtcgc cgaccaccgc ttcgcgtacc ttgctccaca ggccgtcagc cccgatcagc
     9241 gcgacgcccg gcagggtttc tccgttggca aggcggacac gcacgctgtc cgccgtctgc
     9301 tcgaaggact cgagcttggc gtcggtgcgc agcgtgatgt tgggcaggtc ccggcaggcg
     9361 tcgagaaaca cctgatgcag gtcggcgcgg taaatcacgc catagggaaa accgtaggtg
     9421 gcgcgggaaa cgtcgcccag cggcacccgc accacttttt ctccggtgcg cacatcgttc
     9481 atgcccaggc cgggcgggaa aaacgagacc ttgctgacca catcggtcag gccaagccag
     9541 gcaaacatct tgaaaatatt cggaccgagc tggatgccgg cgccgatttc gccgaagctg
     9601 gcggcctgtt ccagaaccgt cacggcatgg ccgtcacgcg cgaggaccag ggccgtcgcc
     9661 aggccgccta tgccgccgcc ggcgatgagg atggggtggg gagatgtcac gggcaatcca
     9721 gtgtctgtag gggagggttg gacagatgat aagtatactt attattaacc cgtacagtga
     9781 ttaccctgag cgtttaaact cgcgccatgt caaaaagctt taacttcggc gaagccccgg
     9841 ggcaccagat ccggcgcgcc catcagttgt cgatcgccct gttcatggac gaaacggccg
     9901 gttttgacgt gacgccggtg cagttcgcga ttctcaacgc cctgatggac gaccccggcg
     9961 aagaccaggt cacgctggcg ggcagggtgg cctttgatgc cgccacctcg ggttcggtga
    10021 taggccggct cgaggccaag ggctgggtgc ggcgcgaggc cgatccgtcg gacaagcggc
    10081 gcaagctgct gtgggtcacg ccggacggcg aaaaagtcgc gctgcagatg aaacgggccg
    10141 tcagccgggt ccagtcgcgc ttgctcgggc cgctcgatgc cgctgaacgc aagcagttcg
    10201 gcgcgctgct cggcaagctg atcgccggcc acgaggacat ggcctgagcc tggctggccc
    10261 cgggtcctgg cccctcaggc cgatttggcc agcagcgtga agtgctcgac caccggcggg
    10321 ccggcaaaaa acggcccgac gatcgtgcgc cactctgcaa aagccgggga ttcgcggaag
    10381 ccgacggtgt ggtcttccag cgtgtcccag aaaatctgca gcacgtagcg ctcggggctt
    10441 tcaatgccct tgttgacctt gaaaccctga aagcctttgg cgccggcgat gacggtgcgc
    10501 aggccgcgct ggatggcttc gtcaaaagca gcctgctggc cgggctggat gcggatgtcg
    10561 gcgagttcga gaatcatggc gtgtcctgtg tgtggatcag tattggctgg ccggtaactc
    10621 taccgtcaaa ccgtcgagtg cggggctcag gcggatctgg caactgagcc ggctggcggg
    10681 cgtggccggt accgccgtga aagccagcat ggcctgctcc tcgccatcgg ccggcggcaa
    10741 ctggctggca aacggttcgt gcaccatcac atggcaggtc gcgcaggtca gggtgccgcc
    10801 acagtcggcc gcgatgccgg tgatgccgcc ggccacggcc gcttccatca ggctttggcc
    10861 ggttttggcc tgaaccgagg tggatattgc gtcggaagct atgaaattga tagtaatcat
    10921 ggatgatttg aacatcagat gcggctgaaa tattcgcgcc gttgccagtc gtccttgtcc
    10981 tgggcctgct catggcgcgc gatttccgac tgcttgatgc gcgtgaagta gtggatgaag
    11041 ctctcgccaa aagcctgcac cagcaccggg tcggcctgta gcgcttccag cgcttctggt
    11101 aggctggtcg ggatgcgggt gccggcgtcg ccatacggtg cgttggtggc caccggcgcc
    11161 ctgagcccgc ggcggatgcc gtccagcccg gcgtggatct gcgacgccag gtacagatac
    11221 ggattggcgg ccggctcgcc gatgcggttt tcgatgcgtg tggccttgtc ctggcactgg
    11281 cccaccacgc gcagcatggc gccgcggttg tcgcggcccc acagcaccga ctgcggtgcc
    11341 agtgcgttgg gcctgaagcg gccaaagccg ttgatcgtcg gtgtgcagaa caccgtcatg
    11401 ccgcgtgcgt gctccagcag gccggccagg tagtgctccc cgagctgtga gagcgtgtac
    11461 tgcgcgtcgg tcggctgggt gcctgctgcg ggcgtctcgc gctggaacag gttggcaccg
    11521 gtctgcaggt ccaccagcga ctggtgcaaa tgccagccgc tggacatgat gttcggaaac
    11581 ggcgggcggc acatgaaggt ggcgtggtaa ccggcgcgac gtagcgcctg cttcacggcg
    11641 ctgcgaaaca gcaccatgtt gtcggcggcg gtcaaggcgt cggtggcctc gaacaccgct
    11701 tcgacctggc tggggccaag ctcgatttcc agcgaatgca ggggcaggcc cagcgcctgc
    11761 gccgtgtgct gcacgatgcg cagcggctcg tcggccatgt caacgcagtt ctccgccagc
    11821 aggttgtagc cggggtggat catcgcgacc cggggcggca ggccgggcca ggccgcctgt
    11881 tcggggtcga gctgatccct ggccccgtcg tcggtgatgc ggtagatgtg aaactcgatt
    11941 tccagcccgc atttcatgcc gtaaccggct tctgccaact gcgccagggc acgctgcaaa
    12001 atgcgccgcg tgtccagctc gacggccgtg ccgtcctgca tccagggctg ggcctggacc
    12061 cagccggtgg ccggtgccca gggcagttgc cgaaagctgg cggggtcggc cagcagcagc
    12121 aggttgctgg cgaactcgaa gcccggcagc ccggctgtgc cgccggcctc gaagactttg
    12181 aaggcggtgc gatccgacgt gtccttgagc agcagggtac tgaccatgcc gacgccgtcc
    12241 tgcatggccc cggcggcagc gccggccacc agggtcttgc cgcgcagcat gccgtggatg
    12301 tcgcaccagg caaaacgcac cagctcgaca ccgctggcgt ccatcagctg cgcggtatgc
    12361 gccagcgcgt cttgccgcgc ggcatcgtgg atgccgcatt gctcggcaaa gctgctcatg
    12421 cgctgacggc ctgctggatg tctttttctt tccagggcgc attggcctgc ttggcccggg
    12481 cctgttcctg ccaccaggcc ggccattcgc cagccggtgc aatgccgtcc accgtgtccg
    12541 gtccgcgcat ctgcgcggcc tgcgtcgcat cggcgatgcc cggtgcaaca ccgccttcct
    12601 gcacggtggc aatcgccttg agcagggcgc gccggttcgc catgatcacc ttgtccgagg
    12661 tgcccaggtg ctcgcgggtg cggtcctgga tggcgcccat gctttcgacc gcccactggt
    12721 cgtgcacatt gatgtcctcc tcgcccatgc ccaggtaggt ctgggtgcgc tgttcctcgg
    12781 cgttgaagcc ccagttgttg tggcgcccgg cgttcgggat gtagtcgggc agcgagatga
    12841 actgctggcg ctgggcgcgc atgctctcct tgtccagcgg gccggcaaaa ctcgtgaaga
    12901 aggagtacca gtaggtgtgg gtgtcgtcca ccggcacatg catctgcgtg atggtcatgg
    12961 tctccgacag cggaatcacg aaggtggccg gaaaaaccgc gttggttacg cgcacgtgcg
    13021 tgagcttgtc gttcatgggc cgcaaggccg tcagttgcat gccccagggt ttggtctcca
    13081 cgctgatctc gggttgggca aactcgcgca tgatgcgtgt catcggccat tgctcgccgt
    13141 cgatggcgcc ggcgctggcg ctgcggaact gcttgcccgc cgcattctgg ccgatgtcct
    13201 ccagcgcctc gtcctgcagg aagcggtgca ggaacgaagg gtgggccggg tcgatgccga
    13261 cttcgaaagc ctgcagccag ttggcattcc acatgccctt gaaggcaaag ctgtgggtgg
    13321 cgggggcggt gaagcagtcg aaggcgggca gcggcggcgg ggtcgagcct tccggtccca
    13381 tccaggcaaa aagcacgccg ctttgttcgc gcaccgggta gctgcgttgc ttcacgcgct
    13441 cgcacagctt gctgccgctg ggttcggccg gggtgtccac gcactggccc gacacgtcga
    13501 acttccagcc gtggaaggga cagcgcaggc cgtcgccgtc ggcccgaccg aacgacaggt
    13561 cggcgccgcg gtgcgggcag tcgcggtcca gcaggccgta gttgccctgg tcgtcgcgga
    13621 acagcaccag gtcctggccc atcaggcgca ccgccttgac cgggcgtttt tccatgcgcg
    13681 ggtccagcgc cgggttgaat tcatcgacca gcgccgccgg ctgccagtag ctgcgcagca
    13741 gggcgccgca gggcgtgccg ggaccgacac gggtgatcaa atcgttctgt tctgcgttca
    13801 tggttagcct tttcttaaaa cggtataccg aaatttgatt tcagtgtaca tttcggttca
    13861 tgaacttgca agagaccgtg ttgatccagt tgcgcgacct gattctgcgc ggcgaattcg
    13921 aacccggcca gcgactggcc gagcagcagc ttgccgaacg cctgggtgct tcgcgcaccc
    13981 ccgtgcgggc cgcgctggtc acgctggagc aggagggtct ggtcgaggcc aatgagaccg
    14041 gcaagttcct ggtgcgccag ttcacgccgc gcgaggtggc cgacgcgatt gccgtgcgcg
    14101 ggcacctgga aggcatggca gcgcgcctgg tggccgaaca cggcgtgtcg cggcagctgc
    14161 agctggacct gcaggcctgc ctcgacgacg gggacagcgc gctggcggcc aacccgctcg
    14221 attacgaaag ttatgcggcc tacgccgtgg tcaacgatcg tttccacgcg ctggtgctgg
    14281 aagccagcgg caaccgggcg ctgcagcgcg cggttgcgct caatgacaag ctgccgtttg
    14341 cgccggcctc ggccatgctg cccatgcagg gcacggtcgc cctggaccgc gactggatgc
    14401 tgtacgcgca ccgccagcac cacatgttgt tcgccgcgct caaggcgggt gagggcgcgc
    14461 gcgcgcaggc gctggcgatt gaacacaccg aagttgcgca aatgaacatg cgcatggcgc
    14521 tggagcggcg cagcgaaacc gaacgggtgc tgccggcgat ccgtctggtg gtgggccgtt
    14581 gaggatggat gcccgcgtga acagccctgc ttacctggcc ctgtccgggg tcggcaaaac
    14641 cttcggcagc ggcgcagggc agaccgaggc cttgggagaa atcgagctgg cggtgcgcga
    14701 aggcgagttc ctcgccatcg tcggaccctc cggctgcggc aagtccacac tgctgcagat
    14761 cgcggccggg ctggcgcagg caacccgggg caccgtgcag ttccatggcg aggaggtcac
    14821 ggcaccgccg ccaggcatcg tctacctgtt ccagcagtac ggcaggtcgc tgttcccctg
    14881 gcgcaccgtg ctcgacaacg tggcgttcgc ggtggagcac ctccccggga tgagccgtgc
    14941 caaggcgcgc gccagcagcc gcctctacct cgacatggtg gggctgggcg atttcgccgg
    15001 ccactacccc gcgcagctgt cgggcggcat gcagcagcgg gtgaccatcg cgcgtgccct
    15061 cgccgcgcag ccacgggtgc tgttgatgga cgagcccttc agttccgtcg atgccctgac
    15121 gcgacttgag ctgcacgccc tcgtgcagga cctctggcgc aagcacggct tcaccgcagt
    15181 actcgtcaca cacgatgcgg acgaggcgat cttcctcgcc gaccgcatcg cggtgctctc
    15241 ggcccggcct gcgcgcgtgg tccgcatgct ggagaccggg ctgccgcggc cgcgcgaccg
    15301 cctcgagacg ccgctgatgc cgcgctacct ggaactgcgg cacgagttgc tcgcgatgct
    15361 gctggggaag gctccatgag cgcggtgacg ccctcgcgca agcggctgca gtcctggctc
    15421 ggcccgattg tgctgctagg gctcgtgctg ctgctggaac tcgccacacg caccggctgg
    15481 gtcaaccccg tgctggttcc tccgccaacg gccgtggcgc agcgagtctt cgcggtcgtc
    15541 acagccggaa gcatttttga accgctcggc cgcacgatgt acctgttggc ggtcgcctac
    15601 acgattggct gcgtgctggg aatcggcctg ggtgtgctca tgggccggtt ccgcccggtg
    15661 cacggactgc tggaaccgct ggtcgaagtc ctgcgcccgc tgcccaagcc ggccttgctg
    15721 ccgccgctga tgctgttcct gggcctgggc gacaccatga agatcacggc cgtctcgctt
    15781 ggcgtcttct tcccggtgct gatcaacacc atccagggcg tgcgcggcgt cgaccccgtg
    15841 ctggtggagg tggcgcgcac cttccagcac tcgcgcgcgg cgctgctgtg gaaagtggtg
    15901 ctgccgtcgg cgatgcctct cgtgctcgcg ggcatgcgca tcgccctggg catcgcgctg
    15961 gtgctggtgg tggtcgccga gatgatggcc ggcaccggcg gcatcggcta cctgatcatc
    16021 gacttgcagc gctctttccg cattgtcgac atgtacgcgt gggtcgtgat cctcgcggtg
    16081 ctgggctacg cgctgaacga agttttcgtc cgcatcgagc ggcgtgccat tcactggtcc
    16141 ggcgcgcgcg ccggctgaaa cgagatccga gtttcccgtg ccccccccaa cccaccagga
    16201 gaagacgtga tgagcaacct ccctctgcgc cggcggacag ccgccgccgc catagcagca
    16261 accgccctcg gcttggctac accactggtg cgcgcccaga cgaccaggat ccgggtcagc
    16321 accatcccga tcatcgacat tgccccgctg caggtcgcga tcgccaaggg ctatttcgcc
    16381 gccgagggca tcgaagtgga cacgacgccg acccaggggg gcgccgccgg catccccgcg
    16441 cttgccgcag ggcaggtgca gatcgtgttc agcaacgtgg tgtcgatcat ccaggccgcc
    16501 aagcagggcc tgggcgtgca gatcatcgcg ccggccagca ccacgggcga cgccccgccg
    16561 gaccaggcgg cgctgatcgc gaagaagggc tcgcccttca agacaggcaa ggacttcgaa
    16621 ggcaagcgga tcgcggtgaa cacccgcaac aacatcatct ggctgtatgc gcgcgcctgg
    16681 gtgcgcgcga cgggcggcaa ccccgacgcg gtgacctacc tcgaagtccc gttcccgcag
    16741 atggtggacg ccgtgcgcgg cgaccgcgcc gatgcggcct ttgccgtcga gcccttcgtc
    16801 tcgggcggcg tcgctgccga catgcaggtg gtgggctacc cctacaccca agtgcagaag
    16861 cgcttgccgg ccgcgcagta tgcggcgagc aagagctaca tcgagcagaa cccgaaggtg
    16921 atcgagggct gggtgcgcgc ctaccagaag ggcgtggact gggtgaacca gaacctgaag
    16981 tccgaagaac tcctcaccat catctccggt tacacccgca tgccaccgca attgatcgcc
    17041 aagcttgggg tgactgcgta cgagaaagcc gtggaccccg ctgcactggt gccggtggtg
    17101 gaccagatgc ggcgcaacgg cctgctggag gggccgttcg acccgaagtc catcctccac
    17161 cgcacggccg tcaaggcctg aagctgaggt gcccatgacg acgcaggaag aggtgctggt
    17221 caccggggac atcgtgacga tggacccggc ccggccgcgt gcgcaggcgc tggcggtgca
    17281 cggcgggcgc atcgtggcgg tgggcacgcg tgacgaagcc cgcgccgcgc tgggccccgc
    17341 accgcgagag gtgcactacg gcgaaggcac ggtggttccc ggcctgatcg acacgcacaa
    17401 ccacatgcag tggaccgcca tccagacgcg gctggtggac ctggcaccgg cccgttcgat
    17461 cgccgacgtg caggaagcga tccgggccta tgcccagcgc aacccggcca agccctggat
    17521 catgagcggc agcggctggc atgtcgtgag tttgcgcgag ggccgctacc cgacgcggca
    17581 ggagctggac gccgcctgcg cggaccggcc cgtgtacctg ccgcgcgttg ggcacgcggg
    17641 cgtggccaac acgctggcac tgcggatggc cggcatagcc tccgacacgc ctgatccgcc
    17701 cggcgggcgc ttcgagcgtg acgcggacgg ccaccccaac ggcgtgctgc tcgagccttc
    17761 ggccttcgag ctggtggcgc gcctcattcc gccgctctcg gtagaggagc aggccgacgc
    17821 cttgcgcgac atgcagcgcg cgtaccatgc cgccggcctc acgggcatca tggaccccgg
    17881 cgtcacgccg gagatgatgc gcctgtacca gatgctttgg cagcgtggcg agctcacggt
    17941 ccgctccgtg gtgatgccgc tgtcggactc cagtctgccg ctggaggtga acctggccag
    18001 catcacgcat gcgtggccga cgaccggctt cggcgacgcg cgcctgaagc ttggtggggt
    18061 gaagctgttc ctggacggcg gcgcgtccct gggcacggcg ctgatgcgcg agccctatcc
    18121 tgatgagcga tgcaactgcg gcatccaggt cacgccgacg gagtcgttcc ggcagatcgc
    18181 gtgggcgtgc gcccggcaag gctggtcgct cggcgtgcac gtggtgggcg gcaaggccat
    18241 cgacatcgcg ctggaggtgt tcgccgagat cgacagggaa attccgttgc gcccgttgcg
    18301 attcagcctg atccacgcct acctgtggcc ggagcagcgg aacatccgcg aagcggcgcg
    18361 catgggcatc acggtggcga cgcagtgctc gatgcagtac accttcgggc cgctcctggt
    18421 gaagcgcttc ggaacggcgc tcatggcgac tgccacgccc gtgcgcagct ggatcgacgg
    18481 cggcgtcgtc gtgggcggtg gctccgactc gccggtcacg ccgtaccagc cgctgctggg
    18541 gctgtggcag gcgcgcacgc gccacatcgc gggaacggac gagcccgtgg gtcgcctgca
    18601 ggcggtgagc ggtgaggagg ctctggcgct ctacacgcgc gatgcggctt acgtgagctt
    18661 cagcgagcac gagcgcggca tgctgcgtcc cggccttctg gcggactgga cggtgctgtc
    18721 ggtcgacccc gttgcgtgcg accccgaggc cctgcgaggc gcacgcgtgc tcgcaaccgc
    18781 ggtcgacggc cgcctggtgt acgaggcctg acgcgtgcgc gcccgctcgt accggctctg
    18841 gggtgtgctg ctgctggtgc tcctgctggt gctttggcag gtgtccgcgt tgtggtgggt
    18901 atcgagcacc agctggccgc cggtgacgga aatcctgaag gcgatcgccg cggggctggc
    18961 caatggcgag ttggcccaag ttttcggttc cacgttgtgg cgaatggcca tcggcttcgc
    19021 catcggttcg gctgcggggg tgctgctcgg catcacgatg gcgaccttcc cgctggcgga
    19081 cgccgcactg cgtcccctcg tggaactgct gcgccccatc cccatgccgg ccatcgtgcc
    19141 gccgctcatc ctgctgctgg gcatcgatca cgcgatgaag gtgttcgcgg tgtccctggc
    19201 ttgctttttt ccggtgctgg tcaataccgt gggcggtgtg cgcgcggtgg acccgaccgc
    19261 cctggacgtc gcgcgcacgt tccaggtggg gcggctgcgc acgctgctgc aggtggtgtt
    19321 gctggccagt ctgccgttca tcctggcggg actgcgcacc agtctggcgc tggcactcat
    19381 cgtgagcgtc gtcgcggaga tgatcgccgg ctccgagggc atcggctact acatcatgac
    19441 catgcagtac gccatgcgac caagcgacat gtacgccgca attttcctgc ttgcggcgat
    19501 gggctacggc ctgaaccttg cgctgctcgc ggttgaacgt cggctgctgc attggtggca
    19561 gcgcggggac gactgagtcc gggttttccc aagggaaacg gctcttcacc gagctccggg
    19621 caatgccttt catggggact gcccctgaca cggcggataa cgcggcaggg tacactcggc
    19681 cgcttttaca gcggagcaat cgcaggtgac gacagacagg gttcctttca cggcccaggg
    19741 cgttcgccgc ggcttcttcg ctgcccaggt gctggcgccg ggcgtcacgc tgtacgccat
    19801 ggtttttggc gtgctcgcca gtgaacgcgg catgagctgg ctgcaggcca tgctgatgag
    19861 cgccgccgtt tattcaggca gcgcgcaact ggcggcgctg cagggctgga cggcgacggc
    19921 cgcggtcacc ccggtcatcg tcaccatcct ggtgatgaac gcgcggtatg tgctctacgg
    19981 tgcggccatc cagccctggc tggccgggct gccgcgccac cagagctggg gctcgctgtt
    20041 cttcctcggc gacggcagct gggcgatggc catgcgcgag tacgacaccg gctaccgcga
    20101 tgcggggtat gtgttcggct ccggcgtggc gatgtttgtg ccctggatcg gcggcacact
    20161 ggtcggccac ctgctggccg gcaatgtgcc gaatccgacg gccttcgggc tggacttcat
    20221 gctggtggct tttgccgcag cgattggcat ggtgctctgg cgcggcaagt ccgacctgtg
    20281 gctggtcgct gcggccgggc tggtggcgct ggtgctacac cggctgctgc cgggcgcctg
    20341 gtacatcgtc ggcgcaggtg tcgcgggcgg gctgatggga gcgtggcgcc atggccgttg
    20401 aatcgacctt tctgctggcg ctggtgggca tggcgctggc gtctttcgcc tgccgcatca
    20461 gcggcttcct gctcatgggt tatgtgcgca tcacgccgcg cgtggaggcc gcgctcaagg
    20521 cgattccgct gtcggtgatg gtcggcatcg cgacgccggc cgccacttcg ggcaagctgc
    20581 cggaactgct ggccctgctg gcggtggggc tggtgatgaa gctggtgcgc aacgatctgg
    20641 ccgccgccgt ggccggcgcg gcgacggtgg ctgtcgcgcg ctggttgaca tagcccccac
    20701 ggttgctcgc ttcgcgtagc gccctgcccc cggggggggc gctgcgcctg cggcccggca
    20761 aagccggatc cgcggcccct gctggtctgg acctctacat cacgatgccg tcattgcggg
    20821 gctttacccg caatccatgc tgcgcgggcc gggctgttat tccttcgcga cctgcagcac
    20881 cagcttgccg aagttctcgc cgttgaagag cttgagcagg gtttccggga agttctccag
    20941 cccgtccacc acgtcttcct tgctcttcat cttgccttct ttcaggtagg ccgccatctc
    21001 ggcgatcgcc tgcgggtagc ggtcggcgta gtcgaacacc acgatgcctt ccatgcgcgc
    21061 gcggttgacc agcagcgaca ggtagttggc cgggcctttg accggcgtgg tgttgttgta
    21121 ctggctgatg gcgccgcaga tgatgatgcg ggcgccgcgt gtgatgcggg tcagcacggc
    21181 atccagaatg tcgccgccca cattgtcgaa atagatgtcc acgcctttcg ggcagtgctc
    21241 tttcagcccg gctttcacgt cgccggcctt gtagtcgatg caggcgtcaa aacccaattc
    21301 cttgacgacc cagtcgcact tggccttgcc gccggcgatg ccgaccacgc ggcagccctt
    21361 gagcttggca atctggccga cggtctggcc gacggcaccg gccgcaccgg acaccaccac
    21421 ggtttcgccg gccttgggct ggcccacgtc catcaggccg aagtagccgg tcatgccggg
    21481 catgcccagc acgttgagcc aggaggtcag gcccatgcgt gggtcgatct tgaacatgcc
    21541 gctgcgcttg atctgggctt ccggaatcag gcagtattcc tgcacatcca gcccggcgtt
    21601 gacatagtcg cccacggcaa aggccgggtt gttcgaggcg atcaccttgc cgacgccgcc
    21661 ggcacgcatg accgcaccga tttccaccgg cgggatgtag cttttgccct cgttcatcca
    21721 gccgcgcatg gccgggtcca atgacaggta aagggttttg accagcacgc cgccggggcc
    21781 gggctcgggc accggctcgg tggtgaaact ccagttggcg cgggtcggca ggccgaccgg
    21841 gcggcttgcc aggcggacct ggtggttgct cagggatgtg atggcgctca tgggtgtctc
    21901 cggggaagta ggcgggcagg aaaacggcaa tggtagtggc ggcctgcccg ggcgtcagtc
    21961 gcgcaggcga ttcgcgccgt gagggctacg tcgaagtcat gcgacgtgcg cgaacggggg
    22021 gatgtattta accaagcagg ggccgcaggc gcagcggccc cctcgggggg cagggagcga
    22081 cacgaagtgc gcgaccgtgg gggccatggt ttatgctttc agccatgaaa ctccataact
    22141 acttccgttc ttccgcgtcc tttcgcgtgc gtattgcgct tgaactcaag ggtttgcgtt
    22201 acgagtacat tcccgttcac attgccaggg gcgaccataa aaaagaggcc tacgccgcac
    22261 tgtcggccga caccctggtg ccgctgctgg aggtggatgg cgaaaaactt tcgcagtcca
    22321 tggccatcat cgaatacctc gatgaaaaac acccggccgt gccgctgctg ccggccgatg
    22381 ccctgggccg cgccaaagtc cgtgcgctgg cgcagtcgat tgcctgcgag atccacccga
    22441 tcaacaacct gcgcgtgctc aagtacctgg tgcgcgagct caaggtcgat gaagaggcga
    22501 aaaacgcgtg gtaccgccac tggtgccgcg aaggcctgga agccttcgag cgccagctgg
    22561 cccccttgcc ggcttcgacc tattgttatg gcgagacgcc caccctggcc gactgctgcc
    22621 tggtgccgca gatcttcaac gcgaaacggt tcaacgtgaa cctcgagggg ctgagccgca
    22681 cgatggcggc gtttgacgcc tgcatggcgc taccggcctt ccagaaggcc cagccctcgg
    22741 cctgccccga caacgagccc tgatcgcgag ctatcttgca ccctgactgg ctgacgcccg
    22801 actggcccgc gccgcccggc gtgcgcgcgg ttttcaccac ccggcagggc ggtgtgtccg
    22861 tcgcgccctg ggacagcatg aacctcggcg accacgtcgg cgatgcgccc gcgcaagtgg
    22921 ccgccaaccg cgccctgctg ggccaggcca tgggggcgaa gccggtgttc ctgcagcagg
    22981 tgcatggcac ggcggtgctt ctgctggacg gcgaaacacc gcagggtctg gcggccgacg
    23041 ctgccatgag ctcgcagcgc ggtctcgcgt gcaccatcat ggtggccgac tgcctgccgg
    23101 tgctgtttgc ccattccgat ggttcggcag tggccgccgc ccatgccggc tggcgcggac
    23161 tggccggtca gcagggcaag ggtattcttg aacaaacttg tgagggtttt agggctctag
    23221 accaatcggg gcgggcgcaa gaagctatca atacaatagc gtggctgggg ccgtgtatag
    23281 ggccgatggc ctttgaagtg ggtgcggagg tgaggactgc gtttgtcgaa ggcgacccag
    23341 cggccgccgc ttgtttcacg gccctggcat cgggcaaata cctcgccaat ctggccggcc
    23401 tggcccgcct gcgtctgcag gccctgggcg tcacgcagat ctacggcaac gacggcacac
    23461 caccctggtg cacggtgggc aatgcgtcaa ggttcttttc gcaccggcgc gacgccggca
    23521 tccagggcaa tggcttcggc accaccggcc gcatggctgc ctgcatctgg cgtgactgaa
    23581 tccgttgcgg acgagtcggt cgccatcgcg ccggcttcag ccgcttcgcg ggccttgatg
    23641 gccttgcgtc ttgccggcgc gcgcatcagg taaaccacca atgccacagg ccccacaccg
    23701 tagagtacaa aggtgacgag ggcgcccagc agcgtgccgg tggaatgggt ggcttcggcg
    23761 acgctcatca tgagcaccac gtacatccag gcaatgacga ttaaatacat aaaaatttgt
    23821 ggggcgtcat gggcagggaa aggttttcca cgatactagt actgcaaccc gaaaatatcg
    23881 aaacatgaaa gcgcgccttc gcacccatgg cggcgttgc
//
LOCUS       DAIDFG010000009        22349 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_9_length_22349_cov_21.478335, whole genome shotgun sequence.
ACCESSION   DAIDFG010000009 DAIDFG010000000
VERSION     DAIDFG010000009.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 22349)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22349)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22349
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9_length_22349_cov_21.478335"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         4354 a         7221 c         7219 g         3555 t
ORIGIN      
        1 cgccgaccaa gcccgaccaa gcataagcta aagcgtttta atagacgcca tcgcgcgcac
       61 gcgggaacat cccgcagatg ctcgcaaacc aaacacccat cgcccgccaa gtcgggcaac
      121 cgctgccacg gtcgattgac ctggttgtca ttcactgcgc cgccactccc agcggcaagc
      181 cgttgcagca gggcaagccc ggctcggtgg gctacatgaa cgcgccccag gtcattgacg
      241 cctggcacgc tgcccgccaa tttcggcgcc gccccgaggc cgttcgcgcc ttcagctcca
      301 agctgccgtc tatcggctac cacttcatca tcgacctgac cggcgaggtc tggtctggcc
      361 gggccttgac cgaggtcggc gcgcatgcgg cagagttcaa cgccaactcg gtgggcatct
      421 gcctggtggg cggcgctgag cgcgaggcca aatacaccgc cgggcaatgg aagagcctgc
      481 aggcggtggt gtccatgctg ctgtcggact acggcatccc ctgcgccgcg cccaagcgcg
      541 tgcccgacaa gacgcgcaag ctgggctaca ccatgttcgg cggcgtctgc ggccaccgcg
      601 acctgtcgcc cgatggcgat ggcgacggcc ttgtcgagcc cttcgagtgg accaagacat
      661 gccccggctt tgacgtgcgc gcatggctgg cacgcggcat gcagcctgag gtcagccaga
      721 tctaccagga gcagcaagca tgagaggacg cacaaacctt aaacataggc aacggggcca
      781 cggcgactac cggctgatca tgtggatcat ctacagcctg ccatacattg cggccgcttt
      841 gatcgcgctg ctcgcctggg cattgtggag gtggctgaaa tgagccccgg acaaaaagcc
      901 tatgcctcct acagcctggc ggttgccggc aagacttacg acggcatgcc actgctcgac
      961 tggtggcagc taaacgcggc caccaaagcc ggctgggccg ctgccgaaaa cacgccggca
     1021 gtcaagccct ggtggaaatc aaaaacgctg tgggtaaacg cggtggtgct ggcactggcc
     1081 gccgctgagt cccagctcaa tgttttgcag ggcgcattgc ccggcgggtt attcacctgg
     1141 ctggccttcg ggctgccggt cgccaatgcc gccctgcgat ttatcaccag cacggcggta
     1201 ggtaagtcat gacctggatg cgcctggcca tcattgccgc cgtgctggct gcgcttgcgg
     1261 ccggggccgc tgccgtgcgc tcgcacctga tcggtgtggg tgaagcccgc acccaggcgc
     1321 ggtgggacaa gcaaaaagcc attgaccagg ccgaaaccct gcgcctggag cgtgagcgca
     1381 gcgccgacca actgatcaag tttcgtaacg cagaaaggat ttccgatgaa caggcaaaac
     1441 gcgagtctct tcgtgaaaag cgcatccgtg ctggcgatgc tgttgctgac cagctgcgca
     1501 gcgccattga caccctcaac cggcgtgacg tgtccaccgc cggcagcgat cccggatcta
     1561 tcgccctcgc tcaaggtgcc gccaccgcaa gagagctttt tggaagctgc aaccaagctt
     1621 tcctcggact ggctgcagaa gctgaccgcc tccgggacca agtagccggc ctgcaggacg
     1681 acgcgatgtt tgtatgtcgc gcacccaaac aaacaaacaa acaaaacgga gagagccctt
     1741 gagccccttt gatctcatct cgtggaaggt cttcaatgac atcgctcatt gggtggcgat
     1801 catcgccctg ggcgtgtggg gctacctgcg caccaaggac aacgacaacg ttatcgccgt
     1861 gaaggccgtc tccagtgagc tggtggcctt catcaaggca agcaccgatg tcaacgagca
     1921 gcagaacacc cgcctcacgg tgctggagga agccgtcaag cacctgccga ccgagcagga
     1981 catggcccat ctggccaatg acgtgtcctc ggtgaaagcg cagatcaacg gcctggccgg
     2041 cctgctcgca cgcatggagc accagatcaa cctggtgcat gaccacctac tcaacaacaa
     2101 caaacaccga tgagcttcca gaaaaccgtc accgaagacc gccgcctgtc gctgctgctg
     2161 gtgctggcgc agtcgcaggg ctaccaggcc aacggcttcc tgctgcgcga tgccattggc
     2221 gacatctacg gccacaacgc cagcatcgac caggtgaccg gcgacattgc ctggctggcc
     2281 gagcagggcc tggtgacttc gcgcgtgatc ggcgaggtga ccctcgccac catgacggcc
     2341 cgtggcgccg atgtagccgc aggccgtgcc agcgtgcctg gcgtcaagaa gccgacgcct
     2401 taaaggcctt tacatggcac cgaaaaccat cgttaagcgc aagccttccc ggcccagctc
     2461 catcacccag cttgacccca agatcaagga agcggtggac acggccgtgc gcgaaggccg
     2521 caccagcatc gaccagctgg tcgagctgat caattccctg ggcggcgagg cctcacgttc
     2581 tgcggttggc cgctacgtca agacagccaa tgagcgcatg gaggaatatc gccaggcgca
     2641 gcagatggcc gccgtctgga tggacaagat cggcgcggag cccaagggcg acgtgagccg
     2701 catgctgctg gagatgctgc gcatggtggc cttcaagagc atcagcgaga ccgagagcgc
     2761 cagccctgaa gacctgatgt tcttgagcaa tgccatcaag ggctttgccc agaccgacaa
     2821 gctggtgatc gacaaagagt ttgccatccg caagctgatc gcggccaagg ccgagaaggc
     2881 cgccgaagaa atcaccaagg gtgcccgcaa actgggtgcg tctgaagaga ccatccagac
     2941 ctggcgcaac aaggtgctgg gagtcgccgc ccaatgatcc tgccgaagct ggcagtcgcg
     3001 gccgcgatcg ccttgtcccc cacgctgacg ccattggccg aggcgatcca gtaccaggcg
     3061 gcgcagcgct cgcccggcat cttcctggac taccagggca agtgggtcac cgacaaggcc
     3121 aaggtcaagg tgtacgagaa gtcgcgccgc atcggcttga gctggggcga ggccgaagac
     3181 gcggtggaat gcgcggcagc cgccaccggc atggacgtct ggtacatcgg ctacaccaag
     3241 gacatggcga tcgagttcat tctcgattgc gcgcagtggg cgcagcacat gcagggcttc
     3301 gcggccgaca tcgagtcggg cgaggagatc ttcatcgacg gcgaagagcg ccagtcggtg
     3361 ctgaaattca gcatcacctt cgccagcggg caccgcatca cggcactgtc atcgacgccg
     3421 cgaaacctgc gcggcaagca gggccgtgtg atcatcgatg aagccgcctt ccacaccgac
     3481 ctggccggcc tgatcaaggc cgcaatggcg ctgctgatct ggggcggcga ggtgcgcatc
     3541 atcagcaccc acaacggcga cgacaacccg ttcaacaccc tggtcaacga catccgcgcc
     3601 ggccgcgtgc cctacagcct gcaccgcacc accttcgatg aagccatccg cgacggcctg
     3661 taccaccgcg tctgccagcg ccaggagatt gaatggacgg ccgagggcga agccaagtgg
     3721 gcggcaacca tccgcgcgca gtacggcgac gacgccgagg aagagctgga ctgcgtgccc
     3781 aagaacggct ctggcgcctt cctcacccgc gagctggtcg aaggccagat gcgcaagggc
     3841 tggccggtgc tgcgtgacca gcgcgcgccg gcttttactt tccagtctga gcagtcgcgc
     3901 atttccaccg ttctcggctg gtgcgaggac aaccttaagc cgctgctggt tgagttgccc
     3961 aggaacgagc tgggttttgt cggtggcgac tttggccgca ccggcgactt aagcgcgttc
     4021 ctgccgctgc tgcagcgcca ggacttgacg cgctatgcgc cctttgcgat cgagctgcgc
     4081 ggcatgccat tccgtcagca gctgcaggtg ctctggtatt tgatggacgg cttgcaccgc
     4141 tttgtcggtg ccgcgctgga tggccgtggg ctcggctcgc agatggctga agagacggcg
     4201 cagcggtatg gcccggaccg aattctctgc atcatggcca cgcaggcctg gtacctggaa
     4261 aacctgcctc cgtacaaagc ggcgttcgag gaccggatgt tcgagctgcc aatggacgcg
     4321 gacattctgg ccgaccaccg cgtgcccaag gtcgtcaagg gcattccgct gattccagat
     4381 ctgcgcaccg ccgatgctca gaaaaagaaa cgccacggcg acaccttcat cgctggcgcc
     4441 aacgcctggt acgccagtcg agtgctggcc ggcaccaatg actacggcta tgaggccgcg
     4501 ccagcccgcc acggccgctg ggatgcaccg ctcaatgacc gcgacaacga tcacgacatg
     4561 ggcgactttc agcgcggtgc ctggtaggcc aacacaaccg agaaacaact atggccatcc
     4621 ttgaccaatt cggcaaagcc tttgaagacg tagccgtggc tcagctccag gagccgcaga
     4681 ccgcgcgcct ggcgcagctg cgctatgagt ttgagaacca ccccagccgg ggcctgacgc
     4741 cgccacgcct ggcaaagatc ctgcaggacg ccgagcaagg cgacctgacg gcccagcacg
     4801 agctgtttgc cgacatggaa gaaaaggacg gccacctttt cagcgagctg cagaagcgcc
     4861 gcatggccgt gcagcagctg gactggacta tcgtcccgcc caagaatgca acggcccagg
     4921 agaaggccca ggccgagttc atcaccgagg tgctggagtc gatggaggac ttcggcgacg
     4981 tgctgttcga catgacggac gcgatcggcc acggctttgc cccgctggag atgacatggg
     5041 gccgggtgga cggcatccag atgccggtga agatcgagca ccggccacag ggctggttca
     5101 agctggcgct cgatccagag atcggccgca acgagctgcg gctgcgcgac aacagcgcgg
     5161 acggtgagcg tctgtggcag ttcggctgga tcatgcacca gcaccgggcg cgcagtggct
     5221 atgtctcgcg cagcggtttg ttccgcgtgt tgacgtggcc cttcattttc aagaactacg
     5281 ccgtgcggga tctggccgag ttcctggaga tctacggcct accgatgcgg ctgggcacct
     5341 acggcgccag tgccaccaag gaagacaagg ccacgctgct gcgcgcagtg gtcggcattg
     5401 gccacaacgc tgccggcatc atccctgccg gcatggcgat cgacttcaag aacgcggcga
     5461 ccggcgacaa caaagccttc gactccatga tcagcctgat ggagcggacc atgtccaagg
     5521 ccatcctggg cggcacgctg accagcagcg aaggcgccaa cggcacgcag gccctgggca
     5581 aggtgcacaa cgaggtgcgc accgagctgc gcaatgccga tgccaagcag atcgcggcca
     5641 ccctcaagcg ccagctgatc tacccactcc tggctttgaa caagggggcg acggacatgc
     5701 gccgctgccc cagcctggtg ttcgataccc aggagccgga agacctgaag ctgtacagcg
     5761 aggccgtgcc caagctggtg gacgtgggca tgcgcatccc ggccaagtgg gcgcacgaca
     5821 agctgaagat cccgcagccg gccgagggcg atgaagtcct ggtcaagaca gcgcctgcag
     5881 cggccgagcc gaccgacccc aaggcaaaac cgaacaatgc gcccaagcct ggcagtgccc
     5941 aactgaaggc ccgtgtgccg gcgggcggcc ccgagggcga tgaactggac gcgcttgcgg
     6001 ccgacatggg cgacgactgg gaagaagtcc tgggcgaaac cctgcagcca gtccagaccg
     6061 cgctggccgc tgccagcacc ctggaggaat tccgcgatgc cctggaggca gcgctgagcc
     6121 aggccaatcc ggccaggctg gccgagctgc tggcgcgcgg ccaattcgcg gcccgcacct
     6181 ggggccagct caacaaggtc aaaaaggccg attgatgcgc cagccaaacg gccgtaaacc
     6241 cgcgccagca ttgaagtttc cgctttggtg ggcggccgtc cggtgccccc aaaccgggcc
     6301 acttcaaatt tcagggcctt tgtggcgcct ttcgccgatc tggctaccac ccccccaaaa
     6361 aatcgccagc gcggctgcta aagcggtctt taaagggtct atgcgccaat ttggggcggg
     6421 gatgggcttg cctttttcgg ctggttttga gcgggtgttg cattaaatgc ccaaaaaact
     6481 ggacttgaag ccggtcctga aggccgtcaa tccggaggaa gccatcgcct atttccggca
     6541 gaagggctac cggatcggct tcgactaccg cgacgtctgg cagcaggaac accaggcagc
     6601 cttcacggtc gccaaggcga tgcagctcga tctgctggcc gagatccgcg agttcgtgga
     6661 cgcagcgatc gcagacggca ccacgctggc cgtcttccag gaagagctga tgccgcgcct
     6721 ggtcaagcgc ggctggtggg gccgccagga gatggttgac ccggacgacg gcgagaccaa
     6781 gctggtgcag ctgggcagcc cgggccggct ggaagtgatc ttcgacacca accttgccac
     6841 cgcctacagc gaagggcaat gggagcgcat cgagcgcaac caggagctgt tcccctttct
     6901 tgagtacgtg cgcagcgcat cggtcaaccc gcgccacacc cacctggcct atgccggcct
     6961 ggtgctgcgc gccgacgact cgttctggca gtcgcatctg ccgatcaagg aatggggctg
     7021 caagtgcagc gtcatccagt acacgcagcg catgctcgac cgcgagggcc tcaaggtcgg
     7081 taaggcgccg cccgaggtga tgcgcgaggt ggtcaacaag cgcacgggcg aagtgatgag
     7141 cgtgccgacc ggcgttgacc cggccttcca ctacccacca ggcggccggc gcgcgcacct
     7201 ggaaaagatg ctgcaggaca agacccaggc cttcaaggcc gacaaaggcg ccaagccatg
     7261 aacgagctaa agcgctttac tagacgcgtg gtgcggcatc gcgcgaggat caacgcctca
     7321 tgaagaaaac cgctgttgcc ctattgacgt tggcgcttgc gctgcaggcc actgcagacg
     7381 tgcacctgtt gccggtgggc gaattcgttg gccgcgacgg ccgtccagga aagggcctta
     7441 catggaagct gtctgaccaa caagggcgca ccctcgccgc caagttgacc gcccgccatg
     7501 cgcgtgtgca gttcaacctg gactacgaac accaatccat gctgagcgag cagaacggcc
     7561 agccggcacc agcgtctggc tgggccactg ccttcgagtg gcgcgacggc gttgggttgt
     7621 ttgccctgaa tgtccagtgg actgccaagg ccaaagaaat gattgaggct ggtgaataca
     7681 aatacatcag ccccgtcatc gtctacgaca agcagaccgg cgaggtgatc gacgtgctca
     7741 acgcgtcgct ggtcaacatc ccctgtctcg atctgagccc tgtcgcgcaa gagcgcgtcg
     7801 cgcggctcaa cgcaagtttt tcaacccacc cggagcaatc catgaatgaa gttctcaaag
     7861 cgctgctcaa ggcgcttggc ctccctgtta ccgacgccac cacggcagag caagccgtca
     7921 cggccgtttc caacctcaag gctgcagcca tgccggccga cctggtgaag acgctgggcc
     7981 tggctgaagc cgcaactcct gccgaagtgg tcactgctgc tgcagctctg aaagccaacg
     8041 ccgacaaggc tgcaagccta accacggaag tggcgacgct gaaagccagc ggcgcaggca
     8101 gcaaccccga cccgacgaag ttcgtttccc tggaatcctt caacgcactc aacggcgagg
     8161 ttgctgccct gcgcgctgca ggtgtggcaa ccgaggttga caagctgatc gaccaggccc
     8221 gggccgaggg caagtgctcc ccggtggtcg agaaggtatg gcgcgacatt ggcaaggtgg
     8281 acattgccca gctcaaggcg ctgatcaaag acacgccggc caaccccgcc ctggccggcc
     8341 agacgcaaac cggcggcaag cctgccgcag cgcttgaccc caaggcgcca ggcacggccg
     8401 aagagctgac catgtgcaaa agcatgggcc tgacgcttga gcagttccgc ggcggcgcag
     8461 aggttgccgc ctgacgcgcc cccactcaac cccctaacta cctggaaaca acatgacagc
     8521 aattgcaaca gagcgcgaca cccagcgccg cgcgggcgac aaggccgctt accccgtgct
     8581 tgccgccacc aaggtgctgg caggcaccct ggtggtactc accgccgcag gctacgccca
     8641 gggcggcgcg gtggcgacca ccctcaaggc cgttggcgtg gccgacgaaa ccgctgacaa
     8701 cacgctgggt gccagcggcg acatcaaggt gccggtgcgc aacgacggct ggttccgctt
     8761 cgccaacagc gcagcgggcg acctgatcac catcgccgaa atcggcacca acgcctacat
     8821 cgtggacaac cagaccgtcg ccaagacgga cggcgtcgcg acccgcagca tcgccggaaa
     8881 aatccgcgac gtggacgcca ccggcgtctg gatcgagttc atctaacccc cctcaactcc
     8941 tggagcaatc aatgaaacgc agcatttttt ctggtggtcg cctgctggcg atcgtggtct
     9001 tcgccgtgct cggcgcgggc atggcctttg cgggccatca cccggcgcac gccggcctgg
     9061 caatggcggc aatttcgatg ccggccaaca gcgaggcctg ggcggccctg gctttcggcg
     9121 ggctgaccat cacgccggcc aacctgacac tgctcaagca gggttttaac gcggccttca
     9181 agggtgcctt taccacggca aagccgatgt gggacaaggt cgccatgaag gtgccgtcct
     9241 cgaccagcga ggaaatctat gcctggcttg gcgcgaacac gcaaatgcgc gagtggctgg
     9301 gtgagcgggt gtaccagaac ctcaagctgc acggctacag catcaagaac aagaagtttg
     9361 aaaacaccgt ggcggtaccc agggacgcca ttgatgatga ccagtacggc acctacacgc
     9421 cgctgatggc aaacctcggc caggacgcgg cgctgcatcc tgatcagttg atcttttcgc
     9481 tgatcgcagc cggcacttcg acgctgtgct atgacggcca gtacttcttc gataccgatc
     9541 acccggtggg tcagtcgggc gcagaggtca gcgtcagcaa ctaccaatct gttggcggca
     9601 acaacccctg gtatctgctc gacatcagca agatgctcaa gcccttcatt ttgcagatgc
     9661 gccgggacta ctcgtttgtc tccaagacgg acctgacaga ccagcacgtc ttcgacaaag
     9721 acgaatatgt ctttggcgtc gatggccggt tgaacgtggg cttcgggctg tggcagcaag
     9781 cctattgcag caaggccgcc ctggacgcag ccggctacgg cgccgcacgc caggccatga
     9841 tgagcgtcaa aaacgatgcc ggcaagccga tgggcatcat gcccggcctg ctgctcgttg
     9901 gccccagcaa cgaaaaggcc gcgctggacg tggtcaccgc cgagcgccag gcaaacggcg
     9961 ccagcaacgt ctaccgcaac accgccgtgg tcgtgacctg cccctggttg ccttgagcgg
    10021 ccactgactg attaactcca ggagaaaact tagatggcaa ccaagaacca aagccgggcc
    10081 gctggcccgg ccacccgagg cctgaaggtc acagcccgcc cggcgagctt tcgccggggt
    10141 ggccacacct tcagtggcga ggcaaagacg attccgctga gcgagctgtc ggccgaacag
    10201 gtcgagcaga tcaccacaga ccccaacctg gtggtgcagg aagtggacat cgagccgccc
    10261 gccgaggcgt agaccgacac aaataagcaa ctgcaaagca gccaagcaaa agccccggtg
    10321 ggtaacacca ccgggccttt tttgaggttt caggaatgtg ctacccaagt gtgcattcct
    10381 taaagcttaa acacccctta aaaccaagtg ccctacgcca ccgccacaga actgctgacc
    10441 cgcttcgacg ccactgagat ttctcagcgc gtcgatcgcg gcgtgccgcg cctggtcacc
    10501 gccgcgctga tgatcgccgt ggccgcagga accagcctgg tgggctaccc ggccgatgcg
    10561 gtcgcccgcg cccaggccgg cctggtggtg gtgcagcgcg ccctgcagga tgccgacgac
    10621 accatcaacg gctacatcag cgccaggtac accttgccga tcgcgccggt gccagcagtg
    10681 ctgcagcggg tggcctgcga gctggcgcgc ttttacctct atgacgatca ggtcacggat
    10741 ctgatcaagg accgccatgc ggcgaacatc aagtggcttg aaagcgtttc aaagggcgtc
    10801 gtcagcctgg gcgcggacgc ggccagcggc gtgcagcccg tcagcagcgc cggcgccgag
    10861 ctggtgaccg gcggcaaggt gtggggccgc gaaagcagtc gggggtttat atgaccggca
    10921 tcgtcatcga agcccgcacc gatggcctgg agggcctggg cggtgcgttt gcacgcatgg
    10981 ccgcgctcgg ccagcagccc cgcccgatct gggaggccat cggccagtac ggcgaaagca
    11041 gcacccggct gcgcttcaaa aaccaggccg gcccggaccg caagcgctgg gtgcccagca
    11101 tccgcgccca ggccagcggc cctggccgga cacttattca gaaggccagg ctgctgcgca
    11161 gcatcagcca caacgcggac aacagcggca ccagctgggg caccaacgtg atttatgccg
    11221 gcgttcacca gttcggcgct gtcatcaagg ccaaatcagc cggcgcgctg cgctttcgca
    11281 tgccgggtgg cggattcgtg acggtcaagc aagtcaccat cccggctagg ccttacctgg
    11341 gcgtcaatgc cgaggacggc cgcgagatgc tggccctgac caatgacgcc atcgacctgg
    11401 ctgcgcgcaa ccgggggggt gcttgatgct tgccgccgcc gaaaacgccc tcattgcccg
    11461 cctgcaagcg caccgcgacg tgaagcgcct ggtgcgcacc gtgggcaccc tgcccaaggt
    11521 gaccagcgat gcgctgctca agcgctacca cgccgacgcg cctgcccttt atatagtgcc
    11581 gggccggttc acggtggcgg acagcctggc caccatgcgt tttaccgtgg ccggcgtggt
    11641 gcgcaacgtg gccggccagg agcaggcccg caagggcgac ggcattgaca ttggctgcga
    11701 cggcctggta acgctggcca tacgcgccct gcacgaccag cgggtgggcg actgcagctg
    11761 gcgggtgacc ggcggcgaga tggtcgatga agaaattttc gaccaggccg gcgtggccgc
    11821 ggtggagatc acgctggaaa gctggccggt cgagatcggc tttgactacg gcgaggcgca
    11881 gatcgccgag ctgccgagct tcacgcgggt gcatgccgac atcgacattc ccccgcaggc
    11941 cggcagcgcc gagcatgcca agtggctggc cacgccgccc gactattcaa ccagccggcc
    12001 agacgccgac ctggacgttc aactccctgg agcaccctga tggaacagat ttttgtgaaa
    12061 cccgcaccgc agcccgaagg ccagccggcc ctgaaggtgc gcaagctgat caacggctac
    12121 cttgccgaag gcggcgagct ggtcaacgcc gagggctact ggctgcgccg cattgccgat
    12181 ggcgatgtgg tgggcgccgc gccgcctgat gagccgcctg aagcgcaagc cgccaagagc
    12241 gtgccgcgca tggtgaaaac caacgcctca aacctgtaac ccgttttaac tggagcatta
    12301 catgccggac aacatcgcgt tcaataccat cccgatcgac gtgcgcacgc caggccagta
    12361 cctggaggtg gacaacagca aggcggtgcg cggcctgccg gccatgaacc ggcgcatgct
    12421 gttcattggc aacaagttgg cggccggcag cgctgccgcg ttgacgctgc agcgcatcaa
    12481 cagccccgca gaagcggccg cgctgtttgg ccgcggctcg gtgctgcacg agatgctggt
    12541 gattgcccgc aacgccaaca aggaaagcga catctgggca atgggcctgg ccgacgacgt
    12601 ggccggcacc gccgccacca agaccctcac ggtcaccggc ccggccacgg aggccggcac
    12661 gctggcgctg tacatcaacg gtgtcaagat caccgttggc gtggcgctca acgatgcggc
    12721 caccgtggtg gccaccgcca tcgcggcggc cgtcaatgcc cgcctggatg cccctgtgac
    12781 cgcagcggca gccctggccg tcgtgaccct caccgcgcgc cacaagggcg cattcaccaa
    12841 cgacattgat gcacgcatca attactaccc cgacgaaaaa acgccggccg gggtggccat
    12901 cgtcatcgcc gctggcgtgg ccggcgccgg caaccccgat gttgcgcctg ccctggcagc
    12961 catcagcctg gaggcctact acacaatcgc cacgccgtac aacgacgcca gcaacgtggt
    13021 caagctggag accgaactgg ccgcccgctg gggcggcatg gacatgcgca cgggccatct
    13081 gttcatcggc atgaccggca cgcacgcggc gctgaccacc tacggcgcgg cgcgcaacag
    13141 cccgcacagc acggtgatgg gtgtaaaaag cgcgccatcg ccaagctacc actacgcggc
    13201 agcgctggcg gcggtgtgcg agttcagcgg cgcgatcgac ccggcgcggc ctttccagac
    13261 gctcaacctg cccggcctgt tgccgcccgc cctgggcgac cgcttcacgc gccaggagcg
    13321 cgacctgctg ctgcgcgacg gcatcagcac cttcctggta gaccagggcg gcaacgtgct
    13381 gatcgagcgg gtggtgacca cctaccaggt gaatgcctac ggcatcgacg atgtgagcta
    13441 cctcgacctg gagaccaagt ggacggtgga ctacatgcgc tttgccttcc gggcacgcat
    13501 cgcgctgcgc ttcccgcgcc acaagctggc tgacgacggc accagctttt cgcctggcca
    13561 ggcggtggcc acgccgaaca tcattcgcgg cgagctgctc gacgtggcgc gccagctgga
    13621 gctggtgggt gtgctggagg gcttcgagca gttcaagaac gacctggtgg tggtgcgcag
    13681 cctggtggac accggccggg tcaactgcat cttgccgccg aatgtggtca accagttccg
    13741 cgtgttcgcc gcgtcggttc aattcattct ttaagagggc ttcaacatga cgcaaattgc
    13801 aggcaaggta tttgtgacca tcaacggcca gcggctgcgc agcaaggagg gcgcaagcct
    13861 ggagaccggc ggcatcgagc gcgaggcggt catcagcgac agcggcgttg atggcttcat
    13921 ggaaaagcac accgcgccca aggtggaatg caaggtcagc ctgaccaatg acgtgcgcct
    13981 ggcggatctg caggccttca aggacggcac cctggtcttc gagaccgaca ccggccgcac
    14041 ctacacgctg atcaacgcgt ggaacgccaa gccgcccaag ctggagaagg gcgaggtgtc
    14101 tctggagttc ggcgctgccg agtgcctgga aggctaaacg ccgcaggcac atgaccgaag
    14161 ccacctgcgg gtggcttttt ccactgaaac accctgcttt taaacagctt taaacacccg
    14221 gagtccatga tgtctgagaa cctgatcaag cccctgaaaa agccctggaa ggtcggcggc
    14281 aaggaagcca gcgagattga agtgcggccg tccaccatga aagacgtgat cgccgccgag
    14341 cagcaggcat cgagctttca gccgaactcg ttcaacgtgc agatggcctg cctgcagatc
    14401 gtgcgcgcgg gcgagttcac cggcccgttc gtggcgagcc acttcatggg catgagtccg
    14461 aaccgcttcg gtgaaattgc ctcggccctg gcggaggccg accgcctggg ggaagacgcg
    14521 tagccaagca taaagacaag cttggcgggg ttgccctgat agccctgaaa tttcattgga
    14581 gccgcgcgga gatcctgagc ctgccgctgg cagagtttga gctgtacctg aacatcctca
    14641 cgaaaccacc tgaatcatga gcaccaccta cgttgtcggc gtccgaatag atggcagtgc
    14701 agccagctat ctgcgcgcca cgcaacaggc gctgcagggt acccgcgctt ttgccaacgc
    14761 cgcgcgggct gagttccagc ggctcagggg cttcatgcag tccacccagg ggcagcttgc
    14821 atcgctgggc ctgggcgtgg ggctggtgca gaccgctcgc aacgcggcgc tcctggaaaa
    14881 aaagctcacc caggttcggc tgacggccgg cgcgtccgtc gcggagcaga gcgaagccta
    14941 ccgcgagatg ttcacgctga tcaaaaagaa cggcggcgcg atcgaggaca cgctggcggg
    15001 ctacaacaac ctgatccagg ccggtctcaa gcacaaggaa gcgctggagg ccaccaaggc
    15061 tgtgagcctg ggcaaggcgg tgaccggcgc gaatgaagac tctctgagcg gcgcgctgac
    15121 ggtgggcgcg gccaactatg gctttgatct ggcgaaggcc ggtgttgcca cgagcatgct
    15181 cgaccgcatg actgtcgccg gccggctggg caatgccgag ctggagaacc tttcgaccat
    15241 cttcccgcgt gtcgcgtcgc gcgcgcaatc ggcgggcatg agctttgaca agacgctggc
    15301 cttcattgag ggcctgtcgc agatcgagcg ccagcccgaa cgcctggcca ccctggctga
    15361 ttcgactctt cgcctgttca ccaatgccgc ttatgccaag gacgccgaga aggccactgg
    15421 cgtcaagttc ttcggcaagg acggtagccg gcgcgactcg atcacggtgc tggaagacat
    15481 gcgcaagggc tttgtgaagc tgaccaccga cgcgcagcgc ttccagtaca tcaataaggc
    15541 ctttggtaaa accgacctgg acacccagcg cggcattttc gcgctgctca acggcaagag
    15601 cctgggtgag atccgcaaat tcgaggaaga gatcaagact gcgggcggca cgctgcagcg
    15661 cgacctgccg gccgccattg ccaacgcagc cgaccaggcc tcgcgcctca agaacacgct
    15721 gcgcgaatcg gccgaagggt ttgcccggcc gatcaacgaa accattgccg gcgcgatcaa
    15781 gtacctgctc aacagcaagg cccagggtgg cctggagttg agcggtggtc agattgctgg
    15841 cggtgcggcc gtgggcgctg cgggcgtgta tgccctatcg cgcatcctgc ctggtgcggt
    15901 cggcaggatt gtcggccgca cgggcggcgt ggccgctggc gtggcccagg gcaaggcgct
    15961 gcaggccgct gccggcgtga cgccggtcta cgtgaccaac tggtctgaaa tgggcggtgg
    16021 cagcggcgcg gccagtctgc tgggcgtcgg tggagccgct gcgggcggcg ctgcggccgt
    16081 cagcgtgctg accaagctca agacgctcgc ggccctggca ccgctggccg gcgcatccaa
    16141 gctgggcgtg gccggcctgg gctacacggc ggccggcgtg ggcctggctg gcgctgccgg
    16201 ctacggcgtg ggcacgggca tctacaaata cggcctggag ggcaacaagg gcggcgaagg
    16261 cattggcgag ctggtggcgc gcttcatgag cctgtttggc aatgaggagg ccaaacgctc
    16321 ggtggccatc aatgacgctt taaagaacag caaggtcggc ggcgagatca cgatccgcgc
    16381 gctgggcaac ccgaacctgg ccgtgcaggt agaagcaaag ccctttaccg gcacccggat
    16441 gaacgccctg ggccagacca tgcgcggggc gggtgagtaa tggcctggcg cgatgagatg
    16501 ggccgcatca ctctgccaga cggcaggcag ctggtggccg gcagttttcg cggtgtgccc
    16561 tttcgcacgg tggacgccga gattcgcgtg ggccggcgca accaggtcaa cgaatacccg
    16621 cagcgcgacc agccctacgt ggacgatctg ggccgcaagg cgcggcgcta cgccgtcgag
    16681 ctgtacgtca tcggcgacaa ctacttcgcc gagcgcaatg cgctgatcga ggcgctggag
    16741 gccaagggcc ccggcgagct gatccacccg cgctacggcg cgctgacggt ttcggtcgat
    16801 ggcgatgccg gtgtcaagga gtcgcctgac cgtggcggca tggcgcgcat cacggtcacc
    16861 ttcgtcgagg acgcggcgaa caacttcccg aagtcggtgg acgacacggt ctcgcaggtc
    16921 gagatcagcg ccaacgcggc cgacgatgcg gccgaggcgg acttcgcgga agagtttacg
    16981 gtcgagggca tcagcgtgct ggccaccgag gcgatcaagg gcctgacatc gagcgtggcc
    17041 ggactgctgg agacggcgcg gctggcgacc agcgttggcg gcctggccac catcgtgcgg
    17101 ctggtgggcg gcctgaccgg caacctggcg gcgctgatcc gcacgcccgt ggtgctggtg
    17161 cagggcctgc gcggcattta tgcccagctg gtgcaagagg tgagccggcc gctttcggcc
    17221 atcgccgagc tgcagcatgt gtttggcggc aacacccgct cgccggccgt ggccttgagc
    17281 ggctccagcc gcgcgcgcag cctgtccaat gacaccgcca gggccgatct gcagcgccgg
    17341 ctgagcttga gcaaccaggc ccgcctgctg gccgtggcga ttaccaacac cgacgtggtg
    17401 gccaccagcg accaggccac ggcgctgcgc gatgccctgg tggcgcagat cgatgccgag
    17461 ctggaagtga acgacccgcc ggcagccgtg gccaaggcct tgacggccat gcgcgcggcc
    17521 gtggtgcgcg acgtggccgc gcgcagcgag ttcttgcggc gccggtctac ctacacgccg
    17581 caggccgtgt tgccggccgt ggtgctggcg caccggatct atcaggacgc ggccagagcc
    17641 gatgagctgg tggcgcgcaa tggcgtggcg cacccggcct ttgtgccggc gcggccgctg
    17701 gaggtgctgc ggtgatgacc ggccaaaacg aatgcacgct gctgattgac ggcaaggctt
    17761 atggcggctg gacgcgcctg gaggtgcagc gcggcattga gcagatcgcg ggcggcttcg
    17821 tgctgcagct gaccagccgc taccccggcg tggatgtgcc gatgcagctg cgcgagggct
    17881 tgccctgcga ggtgcgcctg ggcgatgacc tggtgatatc gggctacacc gacgattacg
    17941 agaccgacga caccgcgacc agctccagcg tgcgcctgtc gggccgtgac aagacggccg
    18001 acctggtgga ctgctcggcc atctacaaaa gcggccagtg gcgcggcgtg aagctggagc
    18061 ggatcgtggc cgacattgca aaggacttca aaatcaaggt ggtggtttcg ccgggccttg
    18121 ataccggtga ggtgttcaag cgcttcgccc tggaagaagg cgagaaggcc tttgacgcga
    18181 tcgaccgggc ttgcaggctg cgcgcggtgc tggtcaccag cacgcccgac gggaacctgc
    18241 tgatcaccac tgccagcacg gtgagcagcg gcgttcgcct ggaagaaggc gtgaacatga
    18301 cgaagttcaa ttcaaaacac tcctggaaag aacgccacag cgagatcatt ctcaaaggcc
    18361 aggtgccggg tgatgaccag gaaaacggcg cggccgccgc gcacctgaag gcgtccgcca
    18421 aggacgcgga aatctcccgg taccggccgc tggtcgtgat cgccgagcac ggcaccagca
    18481 acaagtccct ggccgatcgc gccgcctggg aggtcaaggt gcgcatgggc cggggcaagc
    18541 gcggcggctg caccgtggtg ggctggcgca ccggcaagga cggccaggag ggcgcgctgt
    18601 ggcagcccaa cacgctggtg catgtgacta gcgagcggat gaaccttgat gcggatctgc
    18661 tgattgtgag ctgcagctac cagttcaccg agcagggcag aatcaccgac ctgacctttg
    18721 cccggccgga ggccttccag ctggtggaag gcatcggccg cagcaagctc aatgcgaagc
    18781 tgagcgacaa gacccagaaa gagaaaaaga aaaagggtga cggcttcaca cccagctggg
    18841 agctggcgcc accgaatccg cgcgacacca ggggcgcacc atgagcatcg gcgaccgcgt
    18901 tcggggcatg atcagccgcg ctgtgatcag cctggtcaac gatgccgcca agctgcaggc
    18961 gctgcaggtc acgctgctgg ccggccagac gcccgacgat gcagagcact tccagcatta
    19021 cggcttcacg agcgtgcccc atgccggcgc ggagggcatt gcgctggcga ttggcggcag
    19081 caccggccac accgtgatca tcaatgtgga tgaccggcgc tatcgggtca agggtctgcc
    19141 aaatggcgaa gtggtggttt acgacgacct gggccacaag gtgcatttga cgcgtgccgg
    19201 catcgtgatc gatggcgccg ggcaggacat ccgcttcatc aatacgccca cggtccgtgt
    19261 agaggctaac ttgcacgtca ccggcgccat cactgcggtg ggcaatgtga ccggtgccgg
    19321 catctccctg cagggccatg tccacggcgg cgtgcaggca ggcgcggcac agaccggcac
    19381 gccggtctga ctaaaccgtt ttaagagaca caagactccc ctttgcccac actcgcacga
    19441 tggacctggc actcatctac aacccgcagc tgcaggcctt cgacattgcg gtcgatggca
    19501 cagacctggc ggccgaggac acgctggcca gcgcggtgct ggtctcgctg ctgtgcgacc
    19561 gcctggctgc ggcctacgag gtcaacgccg gcgaagaccg gcgcggctgg tgggccgacg
    19621 cctttgcgga caaccagcac ctgaccggct cgcgcctgtg gctgctggag cgcgagaagc
    19681 aactgcctgg cgtggtactg cgttgcaagc agtactgcga agaggcgctg caatggatgg
    19741 tcgatggcgg cctggcctcg gccatcacgg tggcagcttt tgtgccgcgc actggctggc
    19801 tggcggcgct gatcaagttc cacgtcaacg gccagacccg cagcttccgt tttgagtttg
    19861 accaggcccg ccaggtctgg cgtcttgccg gggagggctt ttaaatgccg attgaacgcg
    19921 acacgctccc gcagctgatc gacaagggcg caaccgaatt cgagagccgc ctgcctggcg
    19981 tgctggcccg cgtgcgcaac agcatggtcg gcgtgctcaa ccgcgttgtc gctggtgcgc
    20041 tgagtacttt gtacaaatat gcggaatacc tcaacgacca ggtgtggcct gacagggcgg
    20101 cgcctgagtt tctgccgctg cacggcgcgc gctggggcac gccacagctg ccagccaatg
    20161 ccgccaccgg caccgcaggc ttcgccggca tcaacggctc tgtgatcgcc ctgggcaccg
    20221 tggtgcagcg ctctgacgcg gtgcagtacg cgaccacggc tgagggcgcg atcgcggccg
    20281 gcgtggcaag catccctgtg gaggcggttg agccgggcca gcttggcaat gcggtcatcg
    20341 gcaccgcgct cacgctgata tcgcccatcg ccggtgtgaa cgctgtcgcc agcgctcaga
    20401 ctgcccttgc aggcggcgca gaccttgagg ccatcgagcc ttggcgtgcg cgcatcctgt
    20461 cacgcatccg caaacctccc cagggcggcg ctgactacga ttacgaaggc tgggcgcttg
    20521 aggtgcccgg cgtcacgcgc gcctgggtct accctggcga gcagggcgcg ggcacggtgg
    20581 tggtgcgctt cgtgcgcgac gacgacgcat ccatcattcc cgatgccggc gaagtggcgg
    20641 ctgtgcaaac ggccattgac gcggtccggc cagtgacggc cacgacctat gtggtcgcgc
    20701 ccatcgccac gccgcagaac tacacgatcc agctgctgcc agacactgcg gctatccgcg
    20761 ctgcggtgga ggcagagctg ctcgccttgt atcgccgcgc gggcaagcca ggcggcacca
    20821 tgctgatcag ccaggagcgc gaggcaatct ctgtcgccgc cggcgagacc gaccatgtgc
    20881 tgaccgtgcc cgccgccaac caggcgcaca ccacgggcca gttgcccatg ctgggagtca
    20941 tcacatgggt gtgaccttgc aggcctggct cgccgcgctg caggccatgc tgccgccagg
    21001 ccgcgccttc acgcgcgagc cggacggcaa cctcaccaag ttgctgagcg ccatcgccgc
    21061 gatgttcctt gccgctcagc taaaactcga agccctgctc gacgaagcag acccgcgccg
    21121 cgcgaccagc atgctgcccg attgggagcg catgctgggc ctgccggacc actgcacgcc
    21181 ggccgatcaa tctacttttg aacgtcagag cgctgcctat cagcgcctgg tcgagcaagg
    21241 cggccagtca gccgcctact tcaccggcct ggctgcgctg ctgggccagc cgggctgcac
    21301 ggtgacagag ctgcgaccga tgaactgcaa tgacgattgc aatgacgcgc tgaacagccc
    21361 tgaggaccgt tttaactggc ggctcaacat ccccgcgcca tccatcggcg cacggccgat
    21421 gaactgcaat gacgactgca gcgaccctct tgacttttac acgccatctt tgatcgagtg
    21481 cccgatcaaa gagcgcaagc ccgcccacac caacgtattt tttgtctaca cggcataagg
    21541 agcctgacca atggatcgca tcaacacagc atctaaggcc gttgaccttt tcggggccgg
    21601 caagcatggc tggaaaaaca gaaatgtcgc cacgggcgtt gagccgactg atttcaacgc
    21661 tgagtggtgc aatggtgtgc aagaggaagc gctggccatc atcgaggggg ccgggattgt
    21721 gcctgatgct gctgttcgga cgcaggtccg ccaggccatc aagcgcctga caggcggcaa
    21781 cgtcaccacg gtcaacttcg cggccagtcc gttcgcgctg accgccgatc atgccggtct
    21841 ggtgctggtg gatgcggcgg cgggtaacgt ggtgatcaac ctgccagcgg ccaacgtcat
    21901 tgcggccaaa ctggaattca gtttcgttcg ttctgatgcc acggccaaca cagtgacagt
    21961 taatcgtgct ggcgcggata gctttcttgg cggcgcgacc agcttcacgc tgacaggtca
    22021 aggtggccat cggactattg cgggcgatgc gaccagcaaa tggataggaa cttcattaca
    22081 agacgttaat ccaggagtaa tttttgccta tgcgggtacc actgcgcccg ctggctcaat
    22141 gatttgcccg atcgctgcca ccaacgtttc gcgagctact tactcagcgc tgtttgctgc
    22201 tattgggacc acatggggtg ttggtgatgg ggcgacaacc tttggcatcc cgtggttccc
    22261 tgcagactac tcgctgcttc aagccaactc caacgttggt acgcagtctg ttggtgctgt
    22321 gatcgcccac gttcacacaa ccggcgtga
//
LOCUS       DAIDFG010000010        22176 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_10_length_22176_cov_19.603815, whole genome shotgun sequence.
ACCESSION   DAIDFG010000010 DAIDFG010000000
VERSION     DAIDFG010000010.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 22176)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22176)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22176
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10_length_22176_cov_19.603815"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         4198 a         7099 c         6777 g         4102 t
ORIGIN      
        1 aaaaatgctg ccgacggaga cgttcacgtc gatgttgctg gaaccgtcca gggggtcgaa
       61 caccagcagg tacttgccgc gcggatactg caccggaatc tggtacggca agtccatttc
      121 ttccgacgcc atgcccgcga ggttgcccgc ccattcggta cgccggataa agacatcgtt
      181 gctcagcacg tcgagttttt tctgcgtctc gccctgcaca ttgacggagc cgccctcggc
      241 aactgcgtgg ttgcccatga cgccccccaa ctcgccaaac gcgaccgcgc gggcaatgcc
      301 tttgcaggcc agcgcaacgt ccagaatcag ggcattgaaa tcgccactgg cacaggggaa
      361 gcggcggcgc tcctcgatca ggaattgggt cagggtggac cgattggcgg ttaatggcat
      421 ggccgttctt tctggaggtt gatgcaggaa ttcagagcga ggcagcctgg cggcgcagct
      481 cgcgcatgac gccgtggtag ccgccattgg catcaggcgc gccaaacacg gcgctgcccg
      541 ccacgcaggt gtcggcgccg gcctgcacga tctcggcgat gttgtcggtt ttcacgccgc
      601 cgtcgacttc cagccagatc ggctggccac cgcacgcctg gtgcgccgct atgcgctgac
      661 gcacggtacg caacttgccc agcgtggcgg ggatgaactt ctggccgcca aagccggggt
      721 tcacactcat gagcagcacc atgtccagct tgtccatgac atggtccagc cattccacgg
      781 aggtggccgg gttcagcacc agcccggcct tgcagccgtg ttcgcgaatc agcgagagcg
      841 tgcggtccac atgcctggag gcctcggggt ggaaggtgat gatgttggcg ccggccctgg
      901 cgaacagcgg aatcagtgcg tccaccggct ccaccatcag gtgcacgtca atcggaatct
      961 gcacatgcgg gcgaatcgcc tcgcagacca gcggcccgat cgtcaggttg ggcacgtagt
     1021 ggttgtccat cacatcaaag tgcaccagat ccgccccggc cgcctcgatc gcacgaacct
     1081 cctcgccgag ccgggcaaag tcggcggaca ggatggacgg cgccagccgg agcatgggtg
     1141 ttgatgaggt gggggatgtc atggtctggc tttccgggga gttgttctgc gttgtcgctt
     1201 cagcgtgagc gggcgaggcc gccgcgcacc tgctcgccat gccagcgccg caattgcgcc
     1261 aggttcacct ggctcagatc aggcaccacc cgcatggcgg ccgagaagtc gtgatgcgcg
     1321 gtgtagcgcg tcggcgtgat caaggtggcc agccccgctg ccgtggccga ctgcaggccg
     1381 ttgccggaat cttcaaatgc cagacactgt gccggcggca acttgagcgc tgccagcatt
     1441 tgcagataga cctgcgggtc cggcttttta accggtgcag tggaagcgtc tccaatggcc
     1501 gtgaagttca agcgccagtc cgggcccacc gcgcggcgca gcaaggccgc gatgttggcg
     1561 ggcgaggtgg tggtggcaat ggccatctgg aggccgtcgg caagggcttc atccatcaac
     1621 cggagcacac cggggcgcaa gctgaccacg ccgtcgttga cggcacgttc gtaagcagcg
     1681 gtcttgagct catgcaggcg attgatcgtg tcctgcagcg ccatcgcctc cacctcgcgc
     1741 agcgcgggct gggtcgtttt ccagtagtgg agcatgcgct ccttgccgcc cgagatattc
     1801 agcagatcgg tgtacaaggg ctcgtcccag tgccaatcca gcccgaactc ggcaaacgcc
     1861 tggttgaagg ccgccaggtg tgcagactcg gtatcggcca gggtgccatc aacatcaaaa
     1921 atcagcgctt gcaacatgcc agccgtcctt gcaaaggggt ggagcgggct actcaaataa
     1981 gtagcgactg atttaattta tcgaacctta cacataaact caaatcatat ttactagaat
     2041 attcatcagg ttaaactgat tattttcgtt attcgtaacc tgccacccac tccggacccg
     2101 cccatgcgcc cttacaccct caagcaaatt cagactttca tcgaggttgc gcgggacaag
     2161 tcggtctcca aagcggcaga gcgtcttttt gtgacccaac cggccgtgtc catgcagatc
     2221 aggcagctcg aagacgcgtt tgggctgccc ctgattgagc cgatcgggcg caatatccag
     2281 ctgaccaggg ccggacagga gtttttggtc cacgccctcg cggccatggc ccggttcaag
     2341 gacctggaag ccaccatggc cgagcatgtg ggcttcagca aagggcggat tgaactggcg
     2401 gtggtcagca ccgccaaata cttcatgccc atgctgctgg tgcgttttgg caagctgttt
     2461 cccggcatcg agctgacact gcgcatcgac aaccgggaaa acatcctggg catgctgtca
     2521 cgcaacgagg cagatctggt ggtgatgggg cgcgccccca acacgctgga ctgcgaagcc
     2581 acggcttttg ccaccaaccc gctgggcatc gtctgcgcgc ccgaccatcc gctgtcacgc
     2641 cgcaagcggg tgcagtttga ggagctcaag gacaccagct ttgtcgtccg ggaaaagggt
     2701 tcgggcacgc gtgccgccat ggagcggctg ttcggccagc acgacatgcc gatgaacgtg
     2761 gtcatggaga tgcccagcaa cgaaaccatc aagcaggccg tcatggcggg aatgggtctg
     2821 agctttctgt cgctgcgcac ggtgcgccac gaactggccg cgggccacct ggcgctggtc
     2881 gacgtcaacg gcatgccact gatgggtcac tggtacgtca cccacctgag ccagaaaaag
     2941 ctctcaccgg cggccaaggc cttcaaggct tttctgatcg agcaaggcgg cccattgatt
     3001 gattcgtggg catgaggcgg cctaattcat aagaattttc tgatttgtaa tcaaataaat
     3061 atgaatgttt cttatgaaca cttgtctcta tagtcagccc caatacccaa cgggctgatc
     3121 aattccgagc agtttgacgg gcccaaccaa ccacaggaga acccgtcatg gaccagtcca
     3181 accgctacgc cgacctgagc ctcaaggaag ccgacctcat caaaggtggc acccacattc
     3241 tggtcgctta caaaatgaag cccaagcctg gcttcggcag ctatctgcaa tgcgccgctc
     3301 acttcgccgc cgagtcctcg accggcacca acgttgaagt ctccaccacc gatgacttca
     3361 cccgcaatct ggacgcgctg gtttactaca tcgatgaagc cacggaagac atgcgcattg
     3421 cgtacccgat ggatttgttt gaccgcaaca tcactgacgg ccgcatgatg atggtgtcga
     3481 tcctgacact gatcattggc aacaaccagg gcatgggcga catcgagcac ggcaagatcc
     3541 acgacattta cttccctgag cgcgccatcc agctgttcga tggtccgtcc aaagacatca
     3601 gcgacctgtg gcgcattctg ggtcgcccga tgaaggacgg cggctacatc gccggcacca
     3661 tcatcaagcc caagctgggc ctgcgccccg agccgtttgc cgctgcggcc taccagttct
     3721 ggctgggtgg cgacttcatc aagaacgacg aaccgcaagg caaccaggtg ttctgcccgg
     3781 ccaagaaggt gtacccgctg gtgtacgacg ccatgaagcg cgcccaggac gaaaccggcc
     3841 aggccaagat cttctcggcc aacatcaccg ccgacgacca ttacgaaatg ctggcccgcg
     3901 ccgatttcat cctggaatcc tttggcccgg acgctgacaa ggtggcgttc ctggtggacg
     3961 gttttgtcgg cggtcccggc atgatcacca ccgcgcgtcg ccagtacccg aaccagtacc
     4021 tgcactacca ccgcgctggc cacggcatgg tcacttcgcc ctcggcaaaa cgcggctaca
     4081 ccgcctttgt gctggccaag atttcgcgtc tgcagggagc ctccggcatc cacgtcggca
     4141 ccatgggcta cggcaagatg gaaggtgaaa acaccgacaa aaacatcgcc tacatgatcg
     4201 agcgcgatga ggcgcaaggc ccggtctact accagaaatg gtatggcatg aagcccacca
     4261 cgcccatcat ctccggcggc atgaatgccc tgcgtctgcc cggtttcttc gagaacctgg
     4321 gtcacggcaa cgtgatcaac acctcgggcg gcggcgctta cggccacatc gacagcccgg
     4381 ctgctggtgc catttccctg cgccagtcgt acgagtgctg gaagtcaggt gcggatccga
     4441 tcgagtttgc caaggagcac aaagagtttg cgcgtgcctt cgagtccttc cccggtgatt
     4501 ccgacaagct gttccccggc tggcgcgaga agcttggcgt tcacaagtaa gccaaggcgt
     4561 agcactcaag acctgaaaac gcctcctccc aagggaggct tttttcttcc tcaaggaaaa
     4621 gccatgacag ccaaagagca gtaccgggtc attgatgagc ccttttacca agtgcaaggc
     4681 aatgaagtcg ctttgtatga agccgcgtac cgggcccgac tgcccgtcat ggtcaagggc
     4741 ccgacgggct gtggcaaatc gcgctttgtc gaatacatgg catggaagct ggacaagcgg
     4801 ctcatcacgg tggcctgcaa tgaggacatg accgcctccg acctgttggg gcgttatttg
     4861 ctggaagcca atggcacgcg ctggctcgat ggcccgctga ccacggccgc gcgcatcggc
     4921 gccatctgct acctggatga aattgtggaa gcgcggcagg acaccacggt ggtgattcac
     4981 ccgctgaccg atcaccggcg ccagttgccg ctggacaagc gcggcgagtt gatcaacgcg
     5041 cacccggatt ttcagctggt gatctcctac aaccccggct accagagtct gatgaaagac
     5101 ctgaagcaat ccaccaagca acgctttgtg ggttttgatt ttgactaccc ggacgccgaa
     5161 ctggaaaccg ccatcgtcgc cactgaaagc ggcacggacc cggacaccgc cgccaaactg
     5221 gtgaagatcg gcagcaccgc acgcaagctc aagggccatg gcctggacga gggcatctcg
     5281 acccggctgc tggtgtatgc cgccaccctg atcaatggcg gcgtcagccc gccggatgcc
     5341 tgccgcatgg cgctggtgcg gccgatcacc gacgacgccg acatccgtgc cacgctggac
     5401 cacgccattg atgccgtctt tgcctgaaga ttgatagcgt gcgcacaggc ccctccaccg
     5461 aacagatgag cgcgtggcgc gcgcagcttg actgcggttt cccgcaggtc gccgatgtat
     5521 ttgccgactg catgagcgag gccgcggcgc tgctcacgcc cgatggcatc catgcctacc
     5581 tggaacaagg ccgtttcctg ggcaaaatgg gccgtggtgt cgagcccgtg ctgatctttt
     5641 tgcaggagtg gccgcaggtt gcagccattc tgggcgagtc ggccctggcg cctgtcatgg
     5701 acgctgttcg cgtgatgacg aagtcgccca acagccatgc gattacaccg tttctgcaat
     5761 cgctggcggc catcgcccgc cggctgccgt cgcaggcgca gatccagcac tacctcgatt
     5821 tgacgctgga ctacatggag cgcaccagcg tctccattca cggcatccac aagacttatg
     5881 ccagccccag cctgccggat ttttttctca agtcgccgca gctgctgagc ctgctgtcga
     5941 tcgaaggctt gcgcaactgg gtcgcctatg gcgtgcgcaa ctacaaccac cacccggagc
     6001 gccagcgcga ttacttcagc ctgcagtcgg ccgacagccg cgctgtgctg caacgcgagc
     6061 ggcacggcac gctgctggtc gaccacacgc gcaagctcga tttgtacctg ctggggctgt
     6121 ggcaggacgc cgaccctctg gtgccttact ctctgggttt tgacgagctg cgcaaaccgg
     6181 ttccctactt cgacccgctc ggcattcggc tgcccgacgt ctatgacgac tgcaaaggca
     6241 tcagcggcct ggaccgctac cgcgccgcgc tggctcacat ggcggcgcac cggcgctgga
     6301 gcggcgccat ttttgccgac aactacagtc cgtttcagcg catggcggtc gagtttttcg
     6361 aagacgcgcg ggtggaaacc ctggccatgc gcgaataccc ggggctgcgc cgaatttttc
     6421 tggcgctgca tccggcgcca gtggaaggcg cctgcgatcc agagacccat tcctgcctgc
     6481 ggcatcgcct ggccatgttg tcgcgcgccc tgctcgacaa gccgcaggca cccacgcacg
     6541 gctaccagga tgcggccctg aatgactttg cccggcgctt tcatgccctt atggaacatg
     6601 gcgaatcgag taccgaagag attgccggtc tcgccctgtc gtacgtcgca aaaacccggc
     6661 gccagagcga ccagcttccc aagaccttct tcaccgatac cgaggtcgat ttccgcgacg
     6721 acaaccgcca cctgtggcgc tttcacgaac tcagtgatga cgaggagatg ttcgaagagc
     6781 atcgcccgtc gattgccgcc gaagacgcca agggcctgcc gccgcgtcac tacccggagt
     6841 gggactacgc cagccagagc taccgcccgg actgggtcag cctgtatgag aggctgcacc
     6901 cgtcaggcaa cgccgccgac atcgaccggc tgctggccaa acacagcgcg ctggccaaac
     6961 gcctcaaacg cctgctggac ctgctcaaac cgcaggacaa ggtgcgtgtg cgttaccagg
     7021 aagaaggcag cgagctcgac ctggacatgg ccatccgctc gctgattgat ttcaaaagcg
     7081 gctgcacgcc cgatccgcgc atcaacatga gccaccggac cgatggacgc aacatcgcgg
     7141 tcatgctgct gctggacctg tcggcctcgc tcaacgaaaa acccgccggc tccgaccaga
     7201 ccatcctgca gctcagccag gaagccgtgt cgctgctggc ctggtcgatc gaacaactgg
     7261 gggacccctt tgccatcgcc ggttttcact ccaatacgcg ccatgacgtg cgctacctgc
     7321 acatcaaggg ctacggcgag cactggaacg acgacgtcaa gggacggctg gcggccatgg
     7381 aggccggcta ctccacccgc atgggggccg ccatgcgcca cgccgcccac tacctgcaag
     7441 cgcagagcgc cgacaagaag ctcatgctga ttctcacgga tggacaaccg tcagacgtgg
     7501 acgcgcagga cgagcgcctg ctgatcgaag atgcacggca atcggtcagg gaactggatc
     7561 agcagggcat cttcagctac tgcatcaacc tcgaccccca ggccgacgcg tatgtgagcg
     7621 acatcttcgg ccatcagtac acggtgatcg accacataga acgcctgccc gagcagcttc
     7681 caaaactgtt catggcgctg acgcggtgac ggctgcgctg cggtacgatg cagcgtcggg
     7741 tcaaccatcc aattgctatt attatgatag ctatctacgc ttatccaacg ggggccaaga
     7801 gccgatatga ctaaaacatt gttacgcagc tgctggcgca tggttttcat gggcctgctc
     7861 atcctcgccc cggcggccca tgccgttgac gtgaacttca aaccggtgac cgacggtgtc
     7921 tacgcctacg tcggcgacaa aggcgcacgc acgtatgaca acgagggcct gaacgccaac
     7981 atcgggctga tcgtgacgcc ggctggcgtg atactgatcg acagtggcgc cacctaccag
     8041 tcggcccgca aaattcagga agccgtgcga cgcgtcacgc cgcagcccat caagtgggtc
     8101 atcaacaccg gcgggcaaga ccaccgctgg ctgggcaatg gctactttct ggcccagggc
     8161 gccgagatca tcgcccacgc caacgcacgg gccgacatgc tggcgcgcgg tggcgaccac
     8221 ctgacgggac tgacaaccgc cctcaaggag cgtgccgacg gcaccgtgcc gaccttgccg
     8281 acccggttca tcgaaggtct tgatacgcca ctgaccttgg gtggcatgac gctggaattg
     8341 cggcaccggg gcggtggcca cacgccggga gacatgatgg tgtggctgcc ctccagaaag
     8401 gtcttgttca gcggcgacat tgtttacgtc aaccgcatgc tcggcgtcat ccccgtgagc
     8461 aacaccaaag cctggctggc cgcactcaag gtcatcgaag acctgcaacc ccgctacatc
     8521 gtgcccggcc acggcgaggt gaccgatctg gccaccgcgg ccgccgacac acgggactac
     8581 ctgctggcgc tgcgaaccca catgaaaaaa gcggtggatg agggaacgga catgagcgcc
     8641 gcggtcaagt ccttcgatgc gcagcccttc atgcggctgc tcaatgccgc cgagctgatc
     8701 ccgggcaacg ccagtcgcac ctatctggag ctggaacgtg aataagccgc tgccggaagg
     8761 catgcgcgca gggcaatcgc ccggcgttta gcaggccgaa aaacagccgt gcctagcagg
     8821 cgacatggtt agcgcgcgcc tgccatcggt caggcgcgca gcgcagcttg cgccagacgc
     8881 aggcgctcat cgagataggc gccgtagaag tcgggaatat agccgcccac catgcgctcg
     8941 gcaatctccg cgccgccgcg cccgaaaaac aggaccgtag gcgcgacctt gatgccccag
     9001 ctgcgggtca ggtcatcatg ggtgagcgtc gcacccttga agtccctgac cagccggcgg
     9061 ctgcgcatgt caacctgcac caccaccagc ccgtgttgct cccgcagcgg gacgaggtag
     9121 ctctcgcggg ccacgataca gaacgggcag ccttccaggc tggccatcac caccagcggc
     9181 ataccttttt tcagggcctg cgcaagttca tcgctcagtg acagcgcagg gggcaaggtg
     9241 gccggcgcgg ccgcagtggc accgcaggca caggctgcaa cgcctgccag cagcaaacgg
     9301 cggcgcgcga gggggtaagc gccggggcct gtggcgccag acatcagcgc ggtcttgggc
     9361 atcacttggt ctcctgttcc agcagcaggt aggtgttgta ggcattcatg cggttggcga
     9421 cacgaaacag cggcaggtgt tcaaaccggg accagtcggt ggcctggtag gcttcttcaa
     9481 agggttcgag attcctggcc gcctgcccca tcgcggtccg gagataaacg aggtagtcgc
     9541 gtgtgagctg catgtccttg cgggcctcgg aagacaaggg gccatgacca ggcacgatga
     9601 cagccgtatc aaacgcgagc aggtcgtcca gcgcgacgat ccagtgccgg ctgtcggcct
     9661 gaccgacgta cggaatgcgg ctgcgaaagg ccaggtcgcc agcgaacagc accttttccg
     9721 agggcagata gaccgccagg tcttccaggg tatgcgacgg gcccacgtgt ttgagctgga
     9781 aacgcacgcc gcccaccacc agttccttgt ccttgtcgat ccactcgtcg gcctccacca
     9841 gccgcgtctg ggcgtcgatc cagggcgcca gctcctggcg cgatgcctcc agccgcagcc
     9901 gggccgtctc cgaattcaga tagtcgcgcg ccgcacggtg cgcgacgatg cgggctccct
     9961 gcgccttgaa ggcctgcagg ccgtaaatgt ggtcggcatg gtagtgggtc acgatcacat
    10021 gcgtgacggg ttgcggtgtc accttgcgga tctcggccat caggcgtgtc gccaatgccg
    10081 gcgaccccag cgcatcaatc accaccacgc ctgccggtgt gaccacaaat cccgcgttcg
    10141 aaatgaagtt ctggttggcc ggtgagccca gggccgatac gccttcgaca taccacgcag
    10201 agggcgacac cttgtgcgcc accatggcag gcaccgggtc ggcgtagacc gtcaaggcgc
    10261 tcagcgctac ccacagcgcg cccaggccgc gccagcaaaa agattgtccc gtcatcgcca
    10321 tgcgtgtccc ccacaaaacc gcaaataccc caactcgcct gttccggtca aaacggcacc
    10381 cttgagcaag atcaaaaggc taccgtgcca ctgaacttac cattaatata agctaatact
    10441 gaattatttg gaaccgtcat gaacctggca agcattcttg agcaatacgg tgagagcgcg
    10501 gtcctggcga tcggcgggct cgtgatcggc gtccttttcg gattttttgc ccagcgttca
    10561 aagttttgcc tgcgtgccgc tgccatcgag ttctggcatc ggaaattcgg tgagaagctc
    10621 tccgtttggc tgctgacatt ttccagcgcc gtggtgggtg ttcagctgct gatcctgaca
    10681 cattcgctcg atgtgagcac agcgcgccag atcgccgcgc gtggcagtct ctcgggggcc
    10741 ttgatcggcg gcctgctgtt cggcgccggc atgatcatga cccgcggttg cgccagtcgc
    10801 ttgctgatct tgtctgcaaa cggcaatttg cgggccttgc tttcagggct gatctttgcc
    10861 gtcaccgccc aggcttcgct ctccgggtca ctggccccct tgcgcaacac catcagcgcc
    10921 tggtggacgg tggacggcgg cccctcgcgc gatttgctgg ccatcactgg cgccggccac
    10981 tggggcgggc ttctttttgg cctggtgtgg cttgccgcag cgctattctt ttcggtgcgg
    11041 agcggctggg gtttgtggaa atgggtgggc ggcataggcg caggcctgat ggtggccgca
    11101 ggctggggct acacccacct cgtgtcggtc accacgtttc aagtggtgca ggttcagggc
    11161 ctgaccttca gcggcccttc cgcggaatgg ctgatgcggg tgctgtcttc acccgcgccg
    11221 gcaatcggtt ttgactttgg tctgctgccc ggggttttca tcggctcgtt tatcggcgcc
    11281 tggattggca aggatctcaa gctggaaggc ttcaccgatg gttacggcat gcgccgctat
    11341 atcgcgggtg ccgtgctcat gggttttggt tccatgctgg caggcggctg cgccgtcggc
    11401 gcgggcatca ccggtggggc catttttgcc ctgacggcct ggatcgccct ggtcggcatg
    11461 tgggctggtg caggcctgac ggatcgcctg cttgacggca agcccatcgg cccgcgcatg
    11521 accacgggac ggcccgccct gacggcgccc tgagcggcgg cgggttgtgc gtgtttaccc
    11581 gcggtacatc gggtcattca ctcgctatat tcatgttgat tgatatctaa cagaggagac
    11641 ccgccatgca catcaggatt caccttgccg cggctttgct catgggaagc gcggcaagcg
    11701 ccgcgttggc tcaggatgca agcgccaccc tgcacacgcg cgccctcgcc gcgacctgcg
    11761 ccaattgcca tggcaccgac ggccgcacgg tggatggctc ggccatcccc tcgctggtcg
    11821 gcatgcccaa gagctatatg gtcatgcaat tcaaggcctt caaggacggc tcacgtccag
    11881 ccacggtgat gcaccagctc agcaagggtt tcaccgatca gcagatcgac agcatcgcca
    11941 cctatttcgc cgcgaccaag cgctaagccg gagaaacacc atgaaaagac gcaattttgt
    12001 tcaggcatcc ctggccctcg gcacactggg cagcatggtc ggctgcgcca ccacgggcac
    12061 cattcccaac cgggccaagg tgctcgtcat tggcggcggc tacggcggcg caacagcggc
    12121 caagtatgtg cgcatgttgt cgaactacaa gatcgatgtg gtgatggtcg agcccaacac
    12181 cgccttcatt tcctgcccga tctccaatct ggtcctgggc ggctccaaga ccatgggcga
    12241 catcaccacg ccctacgaca aactcagcgg caaacacggt gtcacggttg tcaaggacat
    12301 ggtcagcgcc atcgatgccg ccaaaaaaac cgtgacgctg gcaggtggcg ccaccatcgg
    12361 ttacgacaag ctgatcctgt ctcccggcat cgacctgatg tgggacagca ttgaaggcct
    12421 gcaggccgcc aatgcgtcgg ggcagatttt gcaggcgtgg aaagccggcc cggaaaccct
    12481 ggcgctgcgc cgtcagctcg aagccatgcc cgacggcggt gtgtatgcca tcaccattcc
    12541 cctggccccc taccgctgcc cccccggccc gtacgagcgg gccagccagg tggccagcta
    12601 cttcaagaag gccaagccaa aatccaaggt cctgatcctg gacgccaatc cggatgtcac
    12661 ctccaaggga ccgctgttca agaaattctg ggcagaaaac taccagggca tgctggaata
    12721 ccgtggcgcc cacaaggcca tcgcggtgga tgccaggacc agcacggtca agttcgaggt
    12781 tcaaaacgat gtcaaggccg atgtactcaa tgtgctgccc accatgagcg ccggtgccat
    12841 tgccatgaag tccggcctgg ccaacgccaa cgcacgctgg tgcaacgtcg actacctgac
    12901 gtttgaatcc actgccgccc gggacgtgca catcattggc gattcaacac tctcagcccc
    12961 actgatgccc aagtccggcc acatggccaa ttcgcacggc aaagtcgtgg cggcggccgt
    13021 ggtggcacag ctctcaggca tgacggtcaa cccgaatccg gtgctgacca acacctgcta
    13081 cagcttcgtc aatgacaagc tggtcgttca cgtggcgtct gtgcaccagt atgtggccgc
    13141 cgaaaaaaca ttcaagacgg tggccggttc agggggtgta tctgccgcgc ccaatgaact
    13201 ggaaggcgtg tacgcctgga actgggcgca gaacatctgg gccgatacgc tattgtgaga
    13261 gggctggcaa cttgtttgcc tctacctctt acctttttcc ctttgccttc cccggcacct
    13321 gccggggatt catttttttt tgaggacgat tcatgcacaa acttattctt tccctggcga
    13381 tcgctgtgtt cgctgccggc gccgtctcgg gctgcgccac caccagccag ggcgcaccgg
    13441 cgtcccagcc cgtcaaggcg gtctatcacc tcaccaccgg cattgaaact gctgcagccg
    13501 cactgaacaa cgtccagaac catttgaacg cagaccccaa agccaggatt gtggtggtca
    13561 ccaacggacc cggtatcgac ttcatgcttc aggacgcaaa ggacagtcgt ggacgcgagt
    13621 tcagctccac cgtcagcaca ctggctggtc gtggtgttga attccgtgtc tgcaagaata
    13681 cgctgacaac gcgcaatatt tcaccggaca agcttctcat ggaagcaaaa attgtcccct
    13741 caggcgtttc agaagcagcc aaactgcaag cccaggaagg cttcgtttac atcaagccct
    13801 gatgccgggc ttcatcggcc atttttttcg tccgtttcga tcgcgatctg cgtgcagatc
    13861 gcccggcaca gcgtcacaat ccggtcattg atgatccggt agaaaatctg gacaccatcc
    13921 cggcgcttgc caatgaagcc gctctgataa agcgtgttca ggtgctggga catattgggc
    13981 tgagtggtgt cgatttccat gagcagctga ctcacgtttt tctcgccatt acacaagctg
    14041 ctgatgatct tcagccgcat tggcgccgac atggcccgaa aaagttctgc ggcagactca
    14101 aaaacctgat cagactcggt gattgaagat gaatctgttc cgcttgcgag cttagccata
    14161 ggtgtcgatt tagcctcaga gaagtcgata ttgttcaatg aattcgaata tacacttgtt
    14221 ctgcaccgtc aagatccggc gccttgggtc aagccctcca tcagccaggt ccacgtcgtc
    14281 ttcgcagcct gtcgcgcggc attttcgact cccttcggtt ggcatcaaca acttcggcgc
    14341 cattcggctt cagcagttcc gccgccccat caagctcgcg gccgcatcag cagatgcctg
    14401 acgaacatac tgcgtggcgg gcagatttat acatacatca acaagtactt tgatactggc
    14461 aacgatcctg cccggcaaaa aaataggcgc ttcgcgtccg actgccggcc ggtgatgaca
    14521 ccgcactgac cgcacggcgg cagcaccttg ccgcatttcg acgtcgcctt cccgactgct
    14581 ttcgccctgc ttgcctgcga cccccaaggc gggtctgcag cgcagcggcc cgcagtcaac
    14641 ggacactgct atgcagtcga attccaatgc gaacgtcaga gactgcgtca gaaaacaaaa
    14701 aaagcggcct tttgaggggc cgcttttgct gcatctacct tgtgaacctg ttgattcaca
    14761 aggaaattgt tctggtggcc tggggcggaa tcgaaccacc gacacaagga ttttcaatcc
    14821 tctgctctac cgactgagct accgggcctg agcctaagat tatatatcag gcggcgcgct
    14881 tgccccgggt caggtcgacg ccgagttgtt tgagcttgcg gtacaggtgg gtgcgctcga
    14941 gtccggtctt ctctgccacg cgggtcatgg agccgccttc cttggccagg tggaattcga
    15001 aataggcttt ttcgaactcg tcgcgtgcat cgcgcagcgg cttttcaagc agaaagctct
    15061 gggtggcctg cgggccgagg tccaccgcca ccggcatgac ctggccggtc atcgcgacct
    15121 ccaccgtcat gtccaccgca tgcagcagtg ccgcaggtgt gaccagcggt ttgcggccgg
    15181 ccgacttgag aaggccctgt tcgaccgcct tgagcagctt ttgcagcgtg atgggtttct
    15241 cgagaaacgc gatcgcgccg atcttggtgg cttccacggc agtgtctatg gtggcgtggc
    15301 cactcatcat gatcaccggc atgctcagaa ggccgctgga cgaccattcc ttgaggagcg
    15361 tcacgccatc ggtgtccggc atccagatat cgagcagcac aaggtcgggc cgggagcggc
    15421 cacgcgcggc gcgggcctgc gcagcgtttt ccgcgagttc gacttgatgc ccttcgtcgt
    15481 tgagaatttc ggagagcagg tcacgtatgc ccagctcgtc gtccaccacc agaatgtttg
    15541 ccatgttgaa acgctacccc tgcctgcagt tgtcaattct gcggtaccca cctcagggcc
    15601 cgcagtgttg ttatgtcaca actgcgaatg ataacgatac ttgagcgccc tgcacgacgc
    15661 cgtcaaccac gcgatttgaa atatcgaccc gtgcgccgtg ttcatcggca atttttttga
    15721 cgacggccag ccccaaaccg gtgcccttga ccttggtcgt gacgtagggc tcaaacgccc
    15781 gtttcaggat gtgttccggg aagccggtgc cgttgtcgcg caccgtcagg cgcactcgct
    15841 tcgaggtctc ggagtactcg gttttgagca gcacgcggcc ctccggccgg ccctcctggg
    15901 cgtcctgagc gttttgcaac aagttgtgaa taacctgccg gatctgttgc gaatctgcct
    15961 tgatgggggg gcaaaccggg tcgagctcgg tgtagaccgg cacgaccgca ttctcgttgg
    16021 cataaagctg catcacgtcg ttgaccagtg cattgaggtc gacgggcttg agttcggcgg
    16081 ccggcaggcg ggcatagtcg cggaattcgt tgaccaggcg tttcatcgca tcgacctggt
    16141 cgacgatggt cttgaccgat ttggtcagca ggatctgctc gggctcggcc accttgccgg
    16201 tgagcttcat ctccagcctc tctgccgaca gctggatggg ggtaagcggg ttcttgatct
    16261 catgggccaa ccggcgcgcc acttcgcccc aggcctgggc acgctgggcc gagaccattt
    16321 cggacacgtc gtcgaacacc agcaggcatt cgctgcccgg catcttggcg ccgcgtgcga
    16381 tcagcgtgat ggcgttgtcg ggcgcgccgg gctgtgcggc attgagttcg aacgactgct
    16441 gccagtgctc cacgtcgtgc agctggttct cgccaaagta ggcgtcgaac tgggcctgca
    16501 cgccttgcgc aaaaacctcc agcccgggaa tgtcgctgag cgactgcccg tggtaagccg
    16561 ccagcggcac acgcaggatg cgcgtggcgc cggggttgct ggtctggatg cggcccttga
    16621 ggtccagcac catgacgccg gcggtgaggt tgtccaggat ggtctgcaga ttggcgcgtg
    16681 cggcatccac ctggtccatg cttttttgca ctgcgccgcg cgcgtcggcc agttgctggg
    16741 tcatgtcggc aaaggatcgg gtcagcccgc ccagttcgtc cttgccctgc agcgccgcct
    16801 tgggcgtcag atcgccctgg gccacctgtc gcaccccttc ggccagcacc agcaggggcc
    16861 gggccagctg gttgcccagc accaccgcca gcagcaccgc gccgaacacg gccaggaaca
    16921 aactgagcgt gagtgtgcct atgtacattt tgcgcaggcc accccgggcc agggcgcgct
    16981 cctggtactc gcggttggct tcctgcacgg cgatggcgtt ggccaccagg ctggccggca
    17041 acacctcggt cacctgcagg aagcggatct cgccgagcag gccgacattg gggttgttga
    17101 tcagcgccag ggccttgatg cggggcggtg ttgcactgcc cgcggcgagg cttgccgcgc
    17161 taccgggcgt ctcggcgcct ggcaaggcca cctcgtcgag cccctcgatc tgcgtggcca
    17221 cgcgctggct gcgggcactg cgcagcagtt gcgccggtgg ccgctcgggc ttgagcgagt
    17281 aacgcgaatc gccggcactg gcaatcatct gcccggaggc cgaccacaac acgacgtcgc
    17341 tgacggcaag ctgctcgcgc agtcgctcca gtgccaggcc ggccgaggca tcgggtgtct
    17401 gcgtcagttg ccccgcggct gcgcgggtct tgttgcccag ctcggtggcc agcgtgtcga
    17461 gcgtgacccg gccgagattc aggccggcgt ccagcgcgcc ctcgaccttc acgtcaaacc
    17521 agctttcgat cgaacgcgag acgaactggt aggacaccac gtaaatcatc atgccgggca
    17581 gcaggcccac cagcgcaaaa atcgcggcca gcttgaccag caggcggctg ccgaatttgc
    17641 ctttgcgcag gcgcaggatc aggcgcacga caatccagcc gatgaccagc agcagcaagg
    17701 cggccaccgc cacattgagc ccgaacagca ggacgtagtt gcgttcgtag agttcgcggt
    17761 tgccggtggc ctgggtcagc aaaaagagca gcaccagccc cagcgcggcc atggtgccaa
    17821 cgccgacacc caccgtccag cgaaaagccg gactgctttt cagcgacgac acatggccct
    17881 tcaggttcac ttctccgcct ctttgacggc ttccctggag aggtctttgg tggcgtcgcg
    17941 ggacaggtcg cgaatcggtt cggcgaccag gtcgcggctg gtctcgacgg tgatcgtcca
    18001 gtctttctgc cccgccacgc cgatctggaa ggggcggggc agctgcgaga gatcgagccg
    18061 gaaacggaaa tcgacgttgt gccggacacc gggctcgact tccgccgcat cggcaatccg
    18121 ccagcgcgaa aagcgctgga tcgacgccac ggcatcgtcg agattgtcgt agttctggct
    18181 gaaggaagcg cgcaagccgg tgacgctgat ggcgccgggc agcacattga ggcgccagcg
    18241 ccgggtcagc ggctgaaagg ccaggcgcat ggtgcgcgtc gccgtggtga tgcgtttgtc
    18301 gtaccagtac cagcggctgc ggaagatgtc ggcctcggcc acgaaaaaca tgggaatgcc
    18361 cttgagcagg gcgtcttcaa ccgcaggggg caggtcgaac tggaggctgg ctgacaggta
    18421 cacaccctcg tcggagcgct ccatcttcaa ctggctcacc tcggtcgcca gcgccgcgtg
    18481 actggcaagc gccatcacag tgcacaccca gaacgtcaga agccgccgca gcaccgagtc
    18541 aggctttgcg tttttcaaaa actgcgtaaa agaaaccgtc gtgctcacct tccaaattgt
    18601 ccgggaagac ggtctccagg gcgccacttt ggggtagcaa atgcccgggt gatggcatga
    18661 aatgggcgtc tctgtggtgc gtaacaaacg tttgggcctg ctgctcacct tccgccctga
    18721 aaaccgagca ggtgcagtac agcagccggc cgcccggccg gagcagcggc cacagcgttt
    18781 ccagcagccg cgcctgcagc gcggccagct gggcgatgtc ggtcggccgg cgcagccagc
    18841 gcacgtccgg gtggcgccgc acaatccccg atgcggtgca cggtgcatcg agcaggatgc
    18901 cgtcataaag ccggccgtcc caccagccgt cggggcgggc ggcatccccc acccggatgt
    18961 ccgcttgcag gcccaggcgc tgcaggttct gcgccatgcg ctcgcagcgc cgcgcgtcaa
    19021 tatcgagcgc cgtgacctcg cagtcggcga gttccagcag gtgtgccgtc ttgccgccgg
    19081 gcgcggcgca ggcatccagc aggcgcaagg gcgcgccacc gacgggggcc agcccgtcca
    19141 gcagcaaggg agccgccatc tgcgccgcgg catcctgcac cgaaaaatgc ccttcggcat
    19201 acccgggcag tgccgtcacc gcgctcggcg tcgccagcat caccccctgc gggcccacct
    19261 gagttgctat gatattcata gctttcagcg cttgcaggta ttgggctggg gtcgtttttc
    19321 ttgtattaac ccggagaatc agcggcgctt tggcattatt ggcatgcagg atggcctgcc
    19381 agctgtccgg gtggtccttg cgcacccggt ctatccacca ctgcgggtgg ttccagaccg
    19441 cctgcggatg tttttccgtc gaagccatca gagcgtcgcg ctcgcgcagg aagcggcgca
    19501 gacagccgtt gatgaagctg gcctggtgca ccgtggcctc gtcgcgtttg gcggcttcca
    19561 cggcctggtc caccagcgtg aaagcggtat agcgggggct gtcgccttcc ggcaggcggc
    19621 aggcagcagt gtcgtctggg ccgctttccg tacaggccag cgccaaagcg acacacaaca
    19681 gggcgtcggc ctccggcggc ggcggccgct gggccagttg ctggcgcagg gcttcggccc
    19741 ggccgagcca gcgcagcgca tgaaaaccca gggcctgcac gccgggccgg agggtggcgt
    19801 cgatgtcttc gagcgcggcg gtcatggagc ggccgccgcg aacggccatc aatagaaaag
    19861 ccgccccctg caactggcgc cacagaggga tttgagaggt cttgttgtct tgcatcgatc
    19921 ctgtgacggc agccccgcgg gcgccgccgt ttcaagccga ccggggcggg cggcatagga
    19981 cctattagac cagtcttggc gcccggtcag gcgagcgtgt ccgtccgcgg atgttgcggg
    20041 cccggttcat tgcagagacc ccttcctgcc ccgcggattc cgggctcaat acagggcgtc
    20101 aggccgggtc ggcgcgatct cgtcgtcggc cagccccggc atggcacgcc cgtaaatccg
    20161 gcggatacgt tcggccagcg gggggtgcga gtccagccag tgcgcctggg cctgatggcc
    20221 gaccagcagc atgtgctgca ccgccggatg aaaactcgcg ctgccgccag cgccctgctg
    20281 actggccacc ttgcgcaaca caccccccag gccgtcccgg ctgcgcgtga actggaccgc
    20341 acgcgcatca gccaaatact cgcgctggcg cgacaccgcc gctttcagcg cgcggcctgc
    20401 cagccagccg agaaaaccgg ccgccagaat ggccaggccc ggcagcgtca gcaccgagcg
    20461 ccgtccgttc tcgtcgggct cacacatgcc ggcgcccagg ttgaagatca actccagccc
    20521 gaacaccatg ccggccagcc gcatgttgag ccgggtatcg ccttcgcgga tatggctcaa
    20581 ctcatgggcc accagtccct gcagttcgtc gcggttcaag ccgtcgagcg caccgcgcgt
    20641 gacggcgatg accgcatcgt cctcgtccca gccggcggca aaggcgttga tgctgctctg
    20701 gcgctccaga accatgacct gcggcacgcg catgctggcg gcaatggcga gttcggacgc
    20761 aatgttgcaa agcttctgct catcaagccg gctggcgggc caggcttcac gggcacccac
    20821 acgccgcgcc agtttttcac cgccaccgtg cagactggac gtttccagcc accagccacc
    20881 cagcacaaag agcagcgtca tgccggtgtt gacgctgaaa aaatacgccg ggtagcccgt
    20941 cccacccggg gtcagcagat gccaggtcag ggccagcgcg gcattgacgg ccagcaccag
    21001 ggcggccacc gtcaaggcga acagcagcag caattgccgg gtttcggcgc gggcccgctc
    21061 ctggtgttca aaaaaacgca tgttggcctg tcgccgccac cctacatctg gatgcgcacc
    21121 gcttcacgct cggccggcgt ttccgtggcc ctgagcatgg ccgacgcggc aaaaccaaac
    21181 aggcgggcaa tcagcaatgc cggaaattgg gcctgcgcat tgttgtagtc gagtaccgca
    21241 tcgttgtagg cctggcgcgc aaaagccacc ttgttttcgg tgctggtcag ctcctcgctg
    21301 agctcgcgga tggtgtcgtc ggccttgagc tcggggtaag cctcggcgag cgcgaacagg
    21361 cgccccaggc tgccgctgag cgcctgttcg gcggcggcca acgccagcac ttccggcgcg
    21421 tgggtggggt ggctgcgcgc agcatcgctg gccgaacgcg cctggttgcg cgccgcgatc
    21481 acggcttcga gcgtctcgcg ctcgtgcagc aggtattttt tggcggcctc caccaggttg
    21541 ggaatcaggt cgtaacggcg cttgagctgc acatcaatct gcccgaaggc gttggcgatg
    21601 atgttcttca ggcgcaccag ccggttgtag gccccgacgg cccagaacag cagcaaggca
    21661 agcacaacaa tcgaaaccag ggactgggtg gacatggggc cttctttcta tcaacagcag
    21721 ccgcgaaccg gcacgggcga ctttagccga caagccgggc cgctgtacag catgccggca
    21781 catccgcgca gcgcgggggc atggcaattc cgtattttca atgaaaaagc gcctcggacc
    21841 cttattgaca gggcgcgagg cgctacgctt tttatagcgt caaaaagcag caattactcg
    21901 ctggcagcac cttcgctggt ctgctcgggc atggcttcga gttcggccgc ttcggcttcg
    21961 gcgatggcac ggcgctcggc gtcgtccatc tggtccttgg ctttgcgtgc ctcgtggtag
    22021 gccatgccgg taccggcggg gatcaggcgg ccgacgatca cgttctcttt caggccgcgc
    22081 agctcgtcgc gcttgcccat gatggcagcc tcggtgagca cgcgggtggt ttcctggaag
    22141 gaagccgcag agatgaatga atcggtcgac agcgat
//
LOCUS       DAIDFG010000011        22131 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_11_length_22131_cov_18.230295, whole genome shotgun sequence.
ACCESSION   DAIDFG010000011 DAIDFG010000000
VERSION     DAIDFG010000011.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 22131)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22131)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22131
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11_length_22131_cov_18.230295"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         4160 a         6906 c         7119 g         3946 t
ORIGIN      
        1 agcgcgtgga ccaggacctg ggcgcgctgg cgcggccttc acgggtgcgt tttgtgtcgg
       61 tgctgccgaa aacccgcagc ggcaagctgc tgcggcgtgc gatccaggcc gtgtgtgagg
      121 gacgagatgc gggcgacctg acgaccatgg acgacccggc ggcactgcag cagatccggg
      181 acctgatggg cgcggcttga acgcccgggc tggctttttg atgaaaaagt agtgcttgcc
      241 cttctggcgc gtgcgccata agctataaaa aatatagcat tccaactgtt tcagcggcct
      301 cgcgcgggtg gagcaggtgg agtcacgcag tcgcagtcgc agtcgcggtc gccagcgacc
      361 ggagtcaggc tacttcttga aaaccaccgc gccggtgatc aagccggccc cggggttctt
      421 ggcgtaggtc aggcccgcat acgcggcatt ggtgccgacg aacatgccct tgatataggt
      481 gccattgaag gacggcgtgc tgccggatgc cgagtccgtg aagttgccga acgaggtgtt
      541 gatggccacc gagatggtat agcccgtctg gaaattgacc gagaaggtcg cgctgttgag
      601 cgtgccggca ataccgtttt gcgtgggggc cgtgttgccc gccagcgtgt agttggcaac
      661 gccgctgtag gacagtgcgg aaagttctgt ctgtgacgtg ggccgtcctg cgacgtagtg
      721 cacgttgtcc agcgagagga cgctcggatc ttctttctga ccggtggtcc acattcccca
      781 gccgatgaag ttggcgtcac tcacattgcc gacagacttg aaactggtca ccggctgcgc
      841 tgtgccggct tccgaccact tcggcgtgtt ggccccggtg tcctgcagct tgagcagttt
      901 gttgtcgctg aaaacggtcg tggctccggt gctggtcgtc agggtgtatg ccgagccgct
      961 gttgacgccg actgccgcga cctggatgtc cgtgaaggtg cgctcgacag gagacgtcgt
     1021 tgtggtggta gttgttttgg tggtggttgt ggtggttgct gcttccactt tttcggtcac
     1081 aacttttatc ttgcccgcct ggtccacctt gtcgcccaca acgacaggat ccttcgtggt
     1141 aacgggcgtc tcgaccttgg gctgttcgct ggtacgcacc ggtggcgtca cattgtcgag
     1201 aaccttcaac gactctcctg ccaccaggcc tatgcagccg ctctggttgc agacttcaat
     1261 tttgtccagc tcgctggtga tgccgagcga gccatccggg ttgtagccga ccttgaagcc
     1321 cgagccgcgg atgccgatgg tggcggtggt ggtggtgagc cggtagttgt cccggttgcg
     1381 tttgccgatc aggccggtga tggcgcgcat gctgccgcgc agcaggtcaa cgaggaagcg
     1441 gtcttccttg gggttgttct ggtccacata gttggcaatc ttgaactcgg tgttgggctg
     1501 cagtgaaacc aggccaccat cggtgaaccg gacctgggcc cggccgttgc caccagtgac
     1561 gatggcgtga ccgctgtcga tgctgccgcc cttggccagg gcgatggtct tgccatccgc
     1621 ctgccggacg ttgacttcac cggcagtgaa ttgggcgatc ccggccagcg catgtacctg
     1681 cagcggccag gcgctcacca gtgccgtcag cagggcggca tttttcagcg ggaagtgtgg
     1741 tttcatgatg tgggccctgg cggatcgttg gatggcggag cggggggggg aaacggcaac
     1801 ggcgggtgcg tctgcctaga aatccttgcg cacggtcacg gacagcacgc gcttgctgaa
     1861 atctgaaatg gagatgttgg agtttgtcct cagcagcgac agctgcggtg tcacgcgcca
     1921 gctcacgaca ggaacccagg tcaggcccag attcaggttg gtctgccggt cacggcggtt
     1981 caccaggaaa tacgggtcgg cgccgccgtg gtggcgcgtt tcatacccgg cgttggcgaa
     2041 caggtccacg tccgcgttga tggactgctg cagaccggcg cgcaggcctg cgagccggtg
     2101 tccgagatgc ggcacgccgg atgcccgctc gttttcggtg ccggcgtaca agccgccaaa
     2161 aaccaccagc ccgccccgaa acgccctcgc gtaggacgtg cccagcacat ggcggttggc
     2221 atcccgcaga ttctggccgg ggtaacgcag ggtggacagc tgcaggtagg tggtccactg
     2281 gctggcggcg tccggccggt gggtccattc gcccaccaag ccgcgctggt cgcgcacggt
     2341 ggtgttgtcg gcggtatagg tcccgatctg gactgccgcg gtgaactcgt ccttgccgac
     2401 ccggtagctg gccccgccgc tcacgtcaaa ctggcgggtg tcgaaatcgt tgtagtcgtt
     2461 gttgaagcgg tagttgccgg aagcgctggc aatcagcgac aggcgcgggt cgatcacgaa
     2521 acgtccggag acgccggcgc ccaggctgta aaaatcgtcc ttcaatttga cactggtcgg
     2581 gcccagggtc agtatcagtc ccccgagcga tggcacggcg acgttggcat tgccaggacc
     2641 gctgttgata ttgctgtcga tgcctgcgac ggtttcgaca taggccctga cagaagacct
     2701 ggcggcctct tcattgcggt cgatggcctg caggaattcg tcgatcttgc gggccacctc
     2761 ggcagggatg gcgtcccgtt ttgtttcttg cagcacttgc cgcgcggcct ggttgtcgcc
     2821 cagcgcaaac agggcgcggc ccagttcggc gcgggcgcgc gagttgcccg gctggaccgt
     2881 cagcacccgt tcaagcgcaa acacggcacg cgtgaactgg ccggtgtcat tggcggcaat
     2941 gcccaggacg gtgtcaaagt cagggtcgcc cgcgcgtttt tcctccacag cggcgagctg
     3001 gtcaaacgcc tgttgcgcct tgcccttttc caccagcgcc agcgattcac gaaccagtac
     3061 gtcggcttcg gcatgcgccg cgccggcaag cgttgcggaa aaccaggcaa cagacagcag
     3121 aaaaatgaag cgtttgaatg acataggcaa cggacccagg aaaaaccatt ggacgccgaa
     3181 ggttttatat cacgcggtaa cacaaaagca gcatttatta cagtgacttt ctccacgcct
     3241 gccgatgttt cggtctgccg gcgtggtttg ggacgcctgg cgttgggtcc agccgtcaac
     3301 tgacagatgc atgacaaaac cgggcgcccg gtggggggcc ctggtttcgt catttcggca
     3361 ttcggtggca caatcaagtg tgtttctgaa ggcccttcct tgccacagtc gcatccgcta
     3421 atcggtcaag ccgtgttgcg gaaggttaat caaccagcca catcctgctg tggccagcta
     3481 atgtttcccc aagggaaacg gaggtcagcg taatgagttc aagcccggcg gcgtcgtccg
     3541 tctattcggc ctatcaaggc aacacctatc tcttcggcgg caatgcgccc tatgtcgaag
     3601 agatgtacga aaactacctt gccaaccccg gcagcgtgcc cgacaactgg cgcgagtatt
     3661 tcgacgccct gcaaaatgtg cccgccgttg acggcagcaa cgccagggac gtgccgcacc
     3721 tgccggttgt caacgctttc gccgaacgtg ccaagcaggg cgtgacccgt gtggtgggtg
     3781 cttccaccgc cgactccgag tggggacgca aacgcaccgc cgtccagcag ctgattgccg
     3841 cctaccgcaa cgtcggtgcc cgctgggccg acctggaccc cctcaaacgc accgagcgcg
     3901 acaacattcc cgagctcgac ccgtctttct acgggttcgc cgatgccgac caggaagcgg
     3961 tgttcaacac cagcaacacc ttcttcggca aggaaaccat gagcctgcgc gagctgctca
     4021 atgcgctgcg cgagacctac tgcggcacca tcggtgccga gtacatgtac atgacggacc
     4081 agaaccagaa gcgctggtgg cagcaaaaac tcgaagccat ccgcagcaag cccaacttca
     4141 cgccggaaaa aaagcgccac atcctggacc gcctctcggc ggcggaaggc ctggagcgct
     4201 tcctccacac caaatatgtc gggcaaaaac gcttctcgct cgaaggcggc gagagcttca
     4261 tcgccagcat ggacgagctg atccagcgcg gcggcagcat cggcgtgcag gaaatcgtga
     4321 tcggcatggc ccaccgcggc cgcctgaacg tgctggtcaa ctcgctgggc aaggtgccgg
     4381 ccgacctgtt tgccgagttc gagcacaccg cccctgaaga ccttccgagc ggcgacgtca
     4441 aataccacca gggtttcagc tccgacgtca ccacccccgg cgggccggtg catctgagcc
     4501 tggcgttcaa tccctcgcac ctcgaaatcg tcaacccggt ggtcgaaggc tcggtgcgcg
     4561 cccgcatgga ccgccgcgcc gaccccaagg gcctgcaggt gttgccggtg ctggtgcacg
     4621 gcgatgctgc gttcgcaggg cagggcgtgg tcatggaaac cctggccctg gccgagaccc
     4681 ggggttactc caccggcggc acggtgcaca tcgtgatcaa caaccagatc ggattcacca
     4741 ccagcgaccc gcgcgacagc cgctccacgc tgtattgcac cgacgtcgtc aagatgatcg
     4801 aggcgcccgt gctccatgtc aacggcgacg atccggaagc cgtggtgctg gcgacccagc
     4861 tcgcgctcga gttccgcatg gagttccaga aggacgtcgt ggtggacatc atctgtttcc
     4921 gcaagctcgg ccacaacgag caggacacgc cggcgctcac ccagccgctg atgtacaaga
     4981 aaattgcgca acaccccggt acccgcaagc tgtacgccga caagctggcg gcccagggca
     5041 tgggcgaggc gttgggtgac gacatggtca aggccgtgcg cgccgcgctg gacgccggta
     5101 aaagcacctt cgacccggtc atcaccaact tcaagagcaa gtacgcggtg gactggagcc
     5161 cctacctggg caagaagtgg accgacgccg gcgacaccgc cattccgatg gccgagtgga
     5221 aacgcctgtc cgaaaagatc agcaccatac cgacgacggt caccgcccac cagctcgtca
     5281 agaaggtgta cgacgaccgc gcagccatgg gccgcggtga cattccggtg gactggggca
     5341 tgggcgaaca catggcgctg gcctcgctgg tggccagcgg ctacccggtg cgcctgtccg
     5401 gtgaggactg cggacgcggc accttcaccc accgccattc ggtgatccac gaccagagcc
     5461 gcgagaaatg ggacaccggc acctatgtgc cgctgcagaa cgtggccgag aaccaggccc
     5521 cgtttgtggt catcgactcc atcctgtcgg aggaggcggt gctggcgttc gaatacggct
     5581 atgcctccaa cgatccgaac acgctggtga tctgggaagc ccagttcggc gacttcgcca
     5641 atggcgcaca ggtcgtgatt gaccagttca tcgcctcggg cgaggtcaag tggggccgtg
     5701 tcaacggcat cacgctgatg ctgccgcacg gctatgaagg ccaggggccg gagcacagct
     5761 cggcgcgcct ggagcgtttc atgcagctgt cggccgacac caacatgcag gtggtccagc
     5821 cgaccacggc cagccagatc ttccacgtgt tgcgccgcca gatggtgcgc aacctgcgca
     5881 agccgctggt catcatgacg cccaagtcgc tgctgcgcaa caaggacgcc acctcgccgc
     5941 tgtccgagtt caccaagggc ggttttcaga cggtgatccc cgacagcggc gggctcaagg
     6001 ccgacaaggt caagcgtgtg gtcgcctgtt cgggcaaggt ctactacgac ctggccaaga
     6061 agcgcgaaga aaagggtgtc gacgacgtcg ccctcctgcg tgtggagcag ctctacccct
     6121 tcccgcacaa ggccttcgcg gccgagctga agaagtaccc gaatgtcacc gacatcgtct
     6181 ggtgccagga cgagccgcag aaccagggcg cctggttctt cgtgcagcat tacatccacg
     6241 aaaacatggc cgaaggccag aagcttggtt attcgggccg tgcggcctcg gcatccccgg
     6301 ccgtgggtta ttcgcacctg caccaggaac agcaaaaagc gctggtcgac ggcgccttcg
     6361 gcaagctcaa gggctttgtc ctgaccaaat aaacaaatgg aaaagggctg cgccggccgg
     6421 cgcaccccat cgaacccaca acccgaaaga attcaaatgg ctatcgtaga agtcaaggtc
     6481 ccgcagttgt ccgaatccgt ggcggaagcc accatgctgc agtggaagaa aaaagttggc
     6541 gattccatcg ccatcgacga aatcctgatc gagatcgaaa ccgacaaggt ggtgctggaa
     6601 gtgcccgcgc ctgccgccgg tgtcctgtcc gagatcctgg tcgccgacgg cggcacggtc
     6661 acggccgaac aactgattgc ccggatcgac accgaaggca aggcctctgc tgctgcacct
     6721 gccgcagcag cgcctgcggc cgccgtcgca gccaccccgg cagccgcgcc cgccgcagct
     6781 gggggcagca tggccggtgt acccatgccc gcagcggcca agctgatggc cgacaaccag
     6841 ctggcctccg gctcggttcc cgggaccggc aaggacggcc gtgtcaccaa gggcgacgtg
     6901 ctgggtgcgg tggccggcgg cgccccgtct gctcctaaat cagtagctgc cagcgcaata
     6961 ccgacgggcg ctccaaccac ttccttgcct caagtggcgg tacctgccaa ggccagcggc
     7021 ctgggtgacc gccccgagca gcgcgtgccc atgagccgcc tgcgtgcccg catcgccgag
     7081 cgtctgctgc aatcgcaatc gaccaatgcc atcctgacaa ccttcaacga ggtcaacatg
     7141 gcgcccgtca tggagatgcg caagaagttc caggaccagt tcaccaagga acacggcgtc
     7201 aagatcggct tcatgagttt ctttgtgaaa gccgccgtgc atgcgctgaa gaaattcccg
     7261 gtgatcaatg cgtcggtcga cggcaatgac atcgtctacc acggctactt cgacatcggc
     7321 atcgccgttg gttcgccgcg cggcctggtg gtgcccatcc tgcgcaatgc cgaccagatg
     7381 agttttgccg acatcgaaaa gaaaattgcc gagttcggga aaaaagcgca ggacggcaag
     7441 ctgggcattg aagaaatgac cggcggcacg ttctcgattt ccaacggtgg caccttcgga
     7501 tccatgttgt cgacgcctat catcaacccg ccgcagtcgg ctatcctggg cgtgcatgcc
     7561 accaaggacc gcgcagttgt ggagaacggt cagatcgtga tccgtccgat gaactacctc
     7621 gccatgagct acgaccaccg catcatcgac ggccgtgaag ccgtgctcgg tctggtggcg
     7681 atgaaggaag cgctggaaga tccggcacgt ctgttgtttg acatctgagg agccatacat
     7741 gtccaaacaa ttcgacgtga tcgtcattgg tggcggtccc ggcggctaca ttgccgccat
     7801 ccgcgccgcc cagctgggtt tcaacgtggc ctgtatcgac gagtggaaaa acgccaaggg
     7861 tggaccggcc ctgggtggca cctgcaccaa catcggctgc attccgtcca aggccttgct
     7921 gcaatcgtcc gaacactacg accatgccgc tcaccacttt gccgaccatg gcatcgaggt
     7981 caagggcctg ggcctggacg tcgcgaagat ggtggcgcgc aaggacacgg tcgtcaagca
     8041 gaacaacgac ggcatcgtct acctgttcaa gaagaacaag gtcaccttct tccatggccg
     8101 gggctctttc gtcgcggcga aggacggtct ttatgacatc aaggtggctg gagccgccga
     8161 ggaaaccctc tccggcaaac acgtcatcgt ggccacggga tcgaacgccc gcgccttgcc
     8221 cggcgcacct tttgatgaag aaaatattct gtccaacgac ggcgccctgc gcatcggcgc
     8281 ggtgcccaaa aagctgggcc tcatcggctc cggcgtgatc ggtctggaaa tgggctcggt
     8341 ctggcggcgc ctcggtgccg atgtgaccat tctggaaggc ctgccaactt tcctcggtgc
     8401 ggtggaccag caaattgcca aggaagcgca caaggccttt gtcaagcaag gcctgaaaat
     8461 cgagcttggc gtcaaggttg gcgagatcaa gtccggcaaa aaaggcgtat ccgtcgcctg
     8521 gaccaatgcc aagggcgagg cccagacgct cgatgtcgac aagctgattg tctcgatcgg
     8581 ccggactgcc aacaccatcg gtctggcgcc ggaaaccgtg ggcctgaagc tcgacgagcg
     8641 tggcgccatc gtggtcgacg aggaatgcaa aaccaacctg cccaacgttt gggctgttgg
     8701 tgatgtggtg cgcggcccca tgctcgcgca caaggcggaa gaagagggcg tggcggtggc
     8761 cgagcgcatc gccggccagc acggccatgt caacttcaac accattccct gggtgattta
     8821 caccaacccc gagatcgcgt gggtggggca gaccgaagag cagctcaagg ccgcgggccg
     8881 ggcctacaag gccggcacct tccccttcat ggccaacggc cgcgcacgcg ccttgggcga
     8941 taccaccggc atggtgaaaa tgctggcgga cgctgccacc gacgaaattc tgggcgtgca
     9001 cattgttggt ccgatggcca gtgagttgat cgccgaatgt gtggtggcca tggagttcag
     9061 ggccagttcc gaggacatcg cccggatttg ccatgcccat ccgtcgctca gcgaggccac
     9121 caaggaggcg gcgctggccg tcgacaagcg gacgctgaat ttctgacgat gtttgccccc
     9181 acgttccccc acttcgtgtg ggtccctgcc ccccaagggg gcccagccgc cttggggcgg
     9241 cccggcggcg gctgatggtc gtcccgcgag ggtcggtgcg ttcggattac gagtctgaac
     9301 tgaagacgcg gggttacacc agtgaccccg cccagttgcg ggcggtggac gcgctggagc
     9361 gttgcgccac cgagtgggcg gcgttcaagg agaagcgttc caatccgctc aagaagctga
     9421 tcaaccggcc cgatattccg cgcggggttt acatgcacgg cggggtgggg cgcggcaaaa
     9481 gcttcctgat ggactgtttt tacaacgcgg ttccgctcaa gcgcaagacg cgcctgcatt
     9541 ttcacgagtt catgcgcgaa gtccaccgtg aactgcgcga cctccagggc acggtcaatc
     9601 cgctcgacga actgggcaaa cgtattgcca aacgttaccg cctcatctgc tttgacgagt
     9661 ttcacgtcgc cgacatcacc gatgcgatga ttctgcaccg cctgctggcg gcgctgtttg
     9721 acaacggcgt gggttttgtc accacctcca attttcatcc cgacgacctg tacccgaacg
     9781 gcctgcaccg tgaccgcatt tttcctgcga ttgcgctgct caagcagcac ctcgaagtga
     9841 tcagcgtgga caacgggacc gactaccgcc ggcgcacgct cgagcaggtc aggctctacc
     9901 acacgccgct ggggccgcgg gccgacgcgg agatggagga aacttttcag cggttggcgg
     9961 agtctcacga cgaatcgccg gtgctgcaca tcgaatcacg gaaaatccac gccagacgca
    10021 aggcaggcgg cgtcgtctgg ttcgatttca aaaccctgtg cggcgggccg cgttcacaaa
    10081 atgactacct ggaaatcgcc acccagtttc acacggtgct gctcagcgac gtaccgcaca
    10141 tgccggtacg catggcgtcc gaagcgcgtc gctttacctg gctggttgat gttctttacg
    10201 atcggcgtgt caaactcatc atgtcggcag aagtcgctcc tgaagcgtta tatacagaag
    10261 gccctctggc ccacgaattt ccccggacag tctcaaggct caatgaaatg cagtctgccg
    10321 agtttctggc gctggagcgc cgaaatgtgg ataccaccct gacatgaaga aactatttct
    10381 tttcctgctg ctttccacac tgggtttcgc ctcaagcgct caaacccagg atgaactgct
    10441 gtcggccgcc accaatcgcg aacggcagcg catcaacgcc gagcgcgcca ccctggaggc
    10501 ggcatttgat gcggaagaag acgcctgtta caagaagttt tttgtcaaca gctgcctcaa
    10561 tgccatcaaa ccccggcgcc gcgaggcgat ggctgacctg aaattcaggg aagttgccct
    10621 caacgagcag gagcgccggc aaaaggcggc cgagcagatt cgccagaccg aggaaaaatc
    10681 atcgcccgag gccgagcggc aagccgccga gcgccgcgcc acggcgctgg aagacaccaa
    10741 ggcccgggaa gagcgtacgc gccagaaggc cagcgaacgt ttcgatttga agcagaacga
    10801 agcagtcaat gcggcagagg ctgcaggcaa ggaccaaggc tccaaggaga gggccaaggc
    10861 gcgcgccgac aagcaggccg ccagtgccaa agaggcccag atttacaaag acaagcagca
    10921 ggaagcgcag gagagaaaag cgacccgcga gcagaggctg cgcgagcaga ccaagccggc
    10981 ggccaagtca ttgcctgcac ctgactgagc ttaagtcagg gtcaggccag ggcctcgagc
    11041 ttgacgatga ccatcgacag gttgtcgccg ccgccatggg cccgcgaccg cgccttctga
    11101 atcaggaatt cggtcgcctc gcgcggcgac agcatggaca aggccgagcc catttcactg
    11161 ggactgaaat agtgccagac accgtcgctg caggcgatca atgagtcgcc cgggccgagt
    11221 tgggggatgt agtgctggct gatcggcggc acctcttcgg tgcccaagca gcccatcagg
    11281 atgttggact gcggatgcac gttggcctct tcctcggtga gttccccttt gtccaccagt
    11341 gtctggacat acgagtggtc ggtggtccgc ttgaccagct tggcgccccg gaaatggtag
    11401 atgcgcgagt cgccggcatg cacccagtgg cagtctccac ccgggttgat caggaacgca
    11461 gccagcgtgc tgtgcggctc ctgttcggcg gaaatggcgg tgagtttgat cacggtgtgg
    11521 gcctcttcca ccagctgttt gagcatggcc accgcatcgt cggtgtcggg ggcataacgc
    11581 tcgaacagct gtttagcggt catcatgacc tggtcggacg ccttgcgtcc gccgcttcga
    11641 ccgcccatgc cgtcggccac gatgccgagc atgcagcccg cgatgcgcgg atggctgagc
    11701 agggccacct ggtcctgctg gtagtcgcgg tctcccttat ggataccggt tgacgccgtg
    11761 attcgataac ctttggacat attgaaatgg ccgagtcatg tggactcgat ttatctccgt
    11821 aatcaacgta ttatcagatg aagctgcccc ccggatcaaa aaacttggac ttcaacctgc
    11881 actccccgga gcgtcgcctc atagacctca agatcgaaca tgcggatctc aatgccctgg
    11941 tggacctcac cgcacatgcc gtgccgatcg acgagttgat gctcaggcgc ctgaaaaaac
    12001 gccgcctgct cttgcgcgac ctgatctccc agctcgagct ggccctcgac cctcccgaac
    12061 ccgcctgatg aacctggaat cgcaagtcct ggaggctttt gcccccgaag gtgtgctggc
    12121 gcgcgcggcc gagcattttg tcccgcgcag cggccagacc gacatggccc tggccgtggc
    12181 gcgcgccatc gaaggcgcct atccgctggt ggtggaggcc agcaccggtg tcggaaaaac
    12241 gttctcttac ctggtgccgg ccctgctcag cggcgagcgg gtgctgctgt ccaccgccac
    12301 caaggccttg caggaccagc tgttcgggcg tgacctgccg gcactggtca aggccttggg
    12361 cgtgccggtc agcaccgcct tgctcaaggg gcgcggcagt tacctctgcc tgcaccggat
    12421 ggagatggcc cgccatgacg cgaactttcc ggagcgcagc tcgcttcgcg cgctggccaa
    12481 ggtggaggag tgggcgcgcg gaaccgatac cggcgatctg gctgaactcc cagggctgga
    12541 cgaacgctcg ccggtgatcc cctgggtgac ctcgacccgc gaaaactgcc tcggggtcca
    12601 gtgcccgcgt tttcgtgcct gccacgtcaa ccatgcacgc cgtgaagcgc tcgcggccga
    12661 cgtcgtggtg atcaaccacc acctgttttt tgcggatctg gcggtgcgcg aatcgggcgt
    12721 agccgaactg ctgcctacag tgcgcattgc gatttttgac gaggcgcacc agctcaatga
    12781 aaccggtgtg cagtttctgg gaaaaaacct gtcgaccggg caactgctcg attttgcgcg
    12841 cgacatgctg ggcaccgggc tgcagctggc gcgcggcctg ctggactggc aggcactggc
    12901 cggcgcgacc gaacatgccg cgcgtgacct gcgcctgtgt gtgggcaagg cctacccggg
    12961 cagcaagctg cgctggaccg atgctgcgcc cgaaggcctg gcccccgtcg aatggggaga
    13021 ggcgctggcg gccgtcagca ccgcgctggc tggcgcctgt gaggccctga acacggtcag
    13081 tgagatcgca ccggacttcg tgcgcctgca cgagcgcgct gccgaactgg aagcgcgcgc
    13141 ccgcgctttt ggtcttgcgc ccgagcccgg cggtgtccgc tggctggatg tgggcaccca
    13201 actgcggctg gtggagtctc cgctggacat cgcccaggct atccaggcca gggtgttcac
    13261 cgacgacaag gcggctgcca aaacctggat ctttacctcg gcgaccttgg gcgacgatgc
    13321 gcaattgagc tggttcacgc agccctgcgg cttgaccggc gccacgacgc tgcaggtggg
    13381 cagccctttc gactatgcgc gccaggcggc gttttacatt ccgcgaaact tccccaagcc
    13441 tggcgagccc acccacacgg ctgctgtggc gtcctccgcg gcggactggg cacgccggct
    13501 gggcggccgc accatggtgc tgacaacgac cttgcgcgcc ttgcgcgcca ttggcgatgc
    13561 cttgcaggcg agctttgaga attcgaccga gatcgaggta ctggtgcagg gggccatgcc
    13621 caagcggacc ctgattgagc gtttccgcca ggccgacagc catggcgggc gtggctgcgt
    13681 gctggtggcg tccgcctctt tctgggaagg catcgatgtg ccgggggatg cgctgcagct
    13741 cgtgatcatc gacaagctgc cgtttccgcc gcccaacgat ccgatggtgg aggccagatc
    13801 gcggctactg gagtctgaag gcaaaagcgc tttcaacgac tatttcctgc cggaagccgc
    13861 ggtggcgctg aagcagggcg cggggcggtt gattcgccgc gagaccgatc agggcgtgct
    13921 ggtggtctgc gacagccgcc tggcttccat gggttacggc aagcggctgc tgcgtgcgct
    13981 gccgcccatg cagcgcctgg cctcggaaga ggagttgaac gagcggctgg atctgcttac
    14041 cagaacttgt accagggccc atccaccgac ttgaagccct tggacaggta ttcgctctgg
    14101 ggaaagcttc tttccatcac gcgtttggtg tcgtcgcgca actgggtcat gttgagggcg
    14161 tcataagact tgtacaggat gaacagggct tcttcgagca ccggcacatc gcggtagtcc
    14221 gcgatcgcag tctgcgcacg gttgatggcg gagatatagg cgccacgggt gtagtagtag
    14281 cgggccacat ggacttcgta ctgggccaag gagttgacga tgtagttcat gcgcaggcgc
    14341 gcgtcggccg aatacttgga ttcgggaaag cggttgacca gctccttgaa ggactcgaag
    14401 gactctttgg cggccttctg gtcccgctcg gacaggtcct gcttgtacag gaagctgaac
    14461 aggcccaggt tctcattgaa gttgaccagg cccttgaggt acagggcata gtcgatcgcc
    14521 gggctggccg ggtgcagctt catgaagcgg tccagcgtcg ccagggcctg tgcctgctcg
    14581 ccacccttga actgggcata ggccttttcc agctgggcct gctgcgccag cggcgtgccg
    14641 gccgcgcggc cttcgagctt ttcaaacagc gggacggcct tgtcgtaggc accggaattc
    14701 gcttcgtccc gggcttccgt gtaaatcttg ttcgggctcc agttggccgt ggggtccttc
    14761 acggtggagg aacatgccga aaggacgaat gcacagacaa gcatggccgc tgccacgggg
    14821 cgtgtggctg gaacaaccga taatttggtg gaaaacatcg caattgatcc tgttaatgac
    14881 tgattctgta caaagcaaac tgattatatc gagcgccact gcggcgggca cgctggtgga
    14941 ggacattctg ggggagggcg agacggccgc cgatgccgag ttgcgcgagg tcagtgttcc
    15001 cgtggagagc cacggcgcgc gtctggaccg ggcactggct ggcattgtgc ccgagttctc
    15061 ccgcagctat ctgcagcaac tgatagaggc tgccgcggtg cagctcaatg gcgtcacggt
    15121 gaccaaggtc tcggccaggg tcaaggccgg cgacaacctc aagatcgaac tcaaacccac
    15181 accgcaaagc caggccttca agccggaggt gatggcgctg gaggttctct ttgaggacga
    15241 gcatctgctg gtcctcaaca agccggccgg cctggtggtg catccggcgc ccggcaactg
    15301 gagcggcacg ctgctcaacg ggctgctggc gcgcgacccc caggccagcc tgctgccgcg
    15361 cgcgggcatt gtgcaccggc tggacaagga caccagcggc ctcatggtgg tggcgcgcag
    15421 gcgccaggtc atggaccagc tggtggtcat gattgccgaa cgaagtgtca gccgggaata
    15481 catcgcgctg gcgcacggcg cgtgggaagg accgaagtcg cgccaggtgg aggcgccgat
    15541 aggccgcgat ccgcgcaatc gcctgcgcat ggcggtggtg gacctggaaa aaacggccgg
    15601 caaaaccgcc tcgaccggct tttacctgct gcaaaacgaa gggccgtttt gcctggtgcg
    15661 ctgccggctg cagaccggcc ggacgcacca gatcagggtg cacatggcct ccatcggcca
    15721 tccgctggtg gcggacgagg tttatggcgg caaacctgcc gcgggcatgg cgcgacaggc
    15781 cctgcatgcc tgccgcctgg ctttcactca cccggtgacc ggccaagccc ttgagttcag
    15841 ggcgccgctg ccggcggatc tggccgcggc gcttggccag ctggggctgg gatacaatca
    15901 aatccagtag ttttctgcta cccgccgcat ggcgcgcccc gatgttgtgg ttgcgcatga
    15961 atgtgaactg cgccgccagg cgcctttatc tcgaatcatt tcggaaccaa gaccatgaat
    16021 acgacggatg ccaagcgcgt cctcgaaacg gcacttattt gcgcgcagca gccgctgcct
    16081 ttgcgtgaaa tgggtgtttt gttcaacggg gcactgaccg ccgacaccct caagctcctg
    16141 ctggaggatc tgcaaaacga ctggtccggg cggggggtgg agctggtgca tgtcgcgacc
    16201 ggctggcgtt tccagagccg gccgcagatg cgcgagtacc ttgatcgcct gcaccctgaa
    16261 aagccgccgc gctacacgcg cgccacgctg gagacgctgg cgatcatcgc ctaccgtcag
    16321 ccggtcaccc gcggtgacat ggaagacatc cggggcgtga ccatcaacag catgattctc
    16381 aagcagctcg aggaccgggg ctgggtcgag gtcatcggcc accgcgaaac cgtcgggcgg
    16441 ccggggctgt acgccacgac gcggcagttt ctcgacgacc tggggctgga gtcgctggac
    16501 cagctgccgg tcatggacac cacggcggcg caggcgtcca tgctcgatca gatcgagttt
    16561 ggcatgccgg cggctgtgac aggcgacgag gcggctcccg aggatgcccg cttgccggac
    16621 gatcccggga ccggcttgct ggtcgcagaa ctttccgatg cggcggatgc cggccattcc
    16681 caggacgctc cgttaaccag cctcacggcc accgatccca acgcctgaac gcaaacgcat
    16741 gaatcaaaaa gacaatccgg ctccggccac cgctcccacc ccccaggttg atgagcccat
    16801 tgtgccgcca gcccccgatt cacttgcgca gggagctcct gatttgatag cggtcgagac
    16861 ggcgcctgcc gacgatgtgg cgaacgcggc gccgggccgg cccgagcgtg cggctcaggt
    16921 gacgccggcg ctggcgcgtt ttgaagacgt ggtgaccggc cagttcgatg ccgacgaaga
    16981 ggaggacgtg gcgctcgagc ttcccaagcg cgtgctgctg ccccagcctg aaacgcccaa
    17041 gctgcacaag gtgctggcgc aggcggggct cggttcccgg ctggaaatgg agcagctgat
    17101 tctggagggc cggatttccg tcaatggaga accggcccac attggccagc gcatccagtt
    17161 tggcgacacg gtcaaggtca atggcaagcc gatccgggtg cgcatcgacc cgccgccgcc
    17221 gcgggtcatc gcctaccaca agccctccgg cgaggtggtg tcgcatgatg acccgcaaaa
    17281 ccggcccacg gtgtttcgcc gtctgcccaa gctgttccag ggcaaatggc aatcggtagg
    17341 ccggctcgac ctgaacaccg aggggctttt gctgctgacc agctccggcg aacttgccaa
    17401 caaactcatg catccccgtt ttggcctgga gcgcgagtac gccgtgcgtg tgctgggtgc
    17461 gctgaacaac gaagagaaaa aacgcctgct cgatggcgtg caactcgacg acggcctggc
    17521 ccagttcagc acgattgaag ccggcggagg cgagggcgcc aactgctggt accgcgtgac
    17581 gatttcagag ggtcgcaacc gcgaggtgcg ccgcatgttc gaagcggtgg gtcacgccgt
    17641 cagccgcctg atccgcatcc gttacggcgc cgtggtgctg ccgcgcggcc tcaagcgcgg
    17701 cgcctttgtc gaactcgacg agcgcgacat tgacgcattg accagcgctg ttgggcgttc
    17761 ggaagcgcgc gccgatccgc gtggagtcaa caggaacgcg ggagaagccg gggcagccgg
    17821 ggaagccggc tccggcgagg gccgtccagg gcgcgggccg cgccgcaatg accgctcacg
    17881 cggcggacgg ggacgccccg acaacggcac cccccgggca ccggatgcgc aggccatgcc
    17941 ggaaaaaagc cggaccgccg agccagacga tcatggcgat gaggccggcc atgatccgct
    18001 ggacctccag ccccccctga atcgcaacga agcgccacgc gaccccaacc gtcgtcgcgg
    18061 cgccaagggt ggacgcggca atgggggcaa ccccggcgcc ggtccccggt ccggtccgtc
    18121 cggaaaattc gggggcaata ccggcggaca ggggccgaag cgccgcgacg accggggcgg
    18181 tgatcgcggc gaccgcggcg acaagggccc cggcggggca cagcccgacc cgatgaaaac
    18241 ctcatttggt tatatcggcg ctgaaacatt tacccgccag cggcaggcgc cgagccaggg
    18301 ccagcgtcgc ggtggcagcg gcacgggcgg tggcggtcct tcaggcccgc ggcgcggagg
    18361 aggaggtggc ggtggcggtg gcggtggcgg gggtggaaac cgcggcggaa atcgcagcgg
    18421 gggcggcaac cgcggcggaa accgttaact caccggcgta ttcacacgca cttttgctca
    18481 agggccattg cccgcgggtt aaaatcagag gctttattcg atttgaataa atcccagaaa
    18541 atcaaggtta aggaaatctc atggcgatcg aacgcactct ctccatcatc aaacctgatg
    18601 cagtggcaaa aaatgtcatt ggtcaaattt atgcacgctt tgaggctgcc ggcctgaaag
    18661 tggttgctgc ccggatggcg cacctgtcgc gcggcgaagc cgaagccttt tatgccgtgc
    18721 ataaagagcg tcctttcttc aaggacctgg ttgagttcat gatttccggc cccgtcatga
    18781 tttccgccct ggaaggcgaa aacgcgatcc tgaaacaccg cgacctgatg ggtgcgacgg
    18841 atccgaagaa agccgccgcc ggtaccattc gcgccgattt cgccgacagc atcgatgcca
    18901 atgccgtgca tggctccgac gccgtcgaaa ccgcccaggt ggagatcagc ttcttcttcg
    18961 cgggcatgaa catctattcg cgctgaagcg cggatttcca tgcaatgacg gccaacctgc
    19021 ttgattacga tctggaaggt ctggccgcct tttgcgggca gctcggagaa aaacgtttcc
    19081 gggctacgca gctttttcgc tggattcacc agaaaggcgc gaccgatttc gaccagatga
    19141 ccgatctggc caagtccctg cgcgaaaaac tcgccggtgc ggcccatatc cagggcctca
    19201 acccggtatc ccggcatgat tcggctgacg gcaccatcaa gtggctgttc gacgtcggtg
    19261 ccggcgatgt cattgaaacc gtttttatcc ccgaggccga ccgcggaacg ctttgcattt
    19321 cgtcgcaggc cggctgcgct gtcggctgcc ggttttgctc caccggacac caaggcttca
    19381 gccgcaacct gacgacagcc gagatcatcg gccagctctg gtatgccgaa cactttctgc
    19441 gcaaacacct tggcaaaacc gagcgcgtca tttccaatgt ggtgatgatg ggcatgggcg
    19501 agccgctgca aaattacgcg cagctgctgc cggccctgcg catgatgctg gacgaccatg
    19561 cctatggtct gtcgcgccgg cgcgtgaccg tctcgacctc gggtgtggtt cccatgatgg
    19621 accgcctggc gcaggattgc ccggtcgcgc tggcggtgtc gctgcatgcg ccggaagacg
    19681 aactgcgcag caacttggtg ccgctgaaca aaaaataccc gatctctgaa ctgctcgagg
    19741 cctgcacgcg ttaccaggcc gcggcaccca gggacttcat cacctttgaa tattgcatgc
    19801 tcgatggcgt caatgaccag ccggaacatg cgcgcatgct ggtggaactg atgcgcaggc
    19861 attcgctgca agggctggcc tgcaagttca acctgatccc tttcaatccg tttccggcct
    19921 ccggcctgaa acgctccgac atgccgcagg tgctggcttt cgccaaaatc ctgatggatg
    19981 ccggcatcgt gaccaccgtg cgaaaaaccc ggggcgacga tatcgacgct gcctgcggcc
    20041 agctcgccgg cgatgtgcag gaccgcaccg gtgtccagca gcgcatggcc acgcagaggc
    20101 aaggtatgct caagggcatc aagatagtct ccagagaggt cggtatatga agcgtgtccg
    20161 caatgtcctg ttgatgtctg tctgctgggc ggccgtgctg ctcgccggct gtgccggcaa
    20221 accgggctcc gccccgggcg ccgcgagcgg gcgcggggaa atcatgacgg agtcggacga
    20281 gccggacgtg cgcaaacgcg cgcgcatccg cctggagctc gccgccaact attttcagca
    20341 ggggcagacc aacgtcgccc tcgacgaact caagcaggcg ctggtgaccg acccgaccta
    20401 tgccgatgcc tacaatctgc gcggcctggt ctacatgcgc atgaatgaca acgcgctcgc
    20461 cgaggacagt ttccggcggg ccatggcgct caatccacgg gacggcgatg tgctgcacaa
    20521 ctatggctgg ctgctgtgcc agcaaaaacg ttacagcgaa tcgatcgcgc tgttcgggcg
    20581 cgctatcgcc atgcctcagt acggcggcca ggccaaaaca ctgatgacac aaggcatttg
    20641 ccagatcagt gcaggccaag cggctgaagg tgagcgcagc ctgttgcagt cgtatgagct
    20701 ggatgcaggc aaccctatta ccggctacca cctgtcaagt gcgcttttcc agcgcggcga
    20761 tctgccgcgt gcgcagtttt acattcgtcg cgtgaataac agcgatctgg ccagagctga
    20821 aaccctgtgg ctaggcatca aggtggagcg cagcctgggc aatcgcgaag cgatgctgca
    20881 gctggccgat cagcttaaaa aacgcttcgg ccagtcacgc gaggccgcag cttacgaaag
    20941 aggagccttc aatgactgac gccgacggcc agctgccggg tagtgctgcg atggcccagc
    21001 aagactcgcc gccccacgtc ctgcagggcc aggcagatgc cgggcccgcg gtcagtgccg
    21061 gctccctgat ccggcaggca cgcgaggcgg caggcctgca tatcgcggct ctggctgtgg
    21121 ccttgaaggt gccggtcaaa aaactcgaag cgctggaggc cgacaggctg gacctgctgc
    21181 cggatgccgt gtttgtccgc gcgcttgcgg caagcgtctg ccgcaccctc aagatcgatg
    21241 cgggtcaggt gctggcgctg ttgccgcaaa ccggcaagcc cagtctcggg cagcagagtg
    21301 cgcccatcaa caccccgttt cgttccccgg gtgatggacc cggtccctcc ctgctggcgc
    21361 agatttccag gcctgccgtt ctggcgggct tgctgctgtt gctgggtgcc ctggtcctga
    21421 tcttcctgcc cgccgtccag cagcccggca ccgccaccca gccggcgaca ccgtcgcccg
    21481 ccgttgccga cacacctgcg gccccgtcgg gcgcgctgtc tggggtggcc atggccagga
    21541 ccgagttgct cgaagtggac aagccccccg tgaccgacag tgcggaccag cccgctacca
    21601 ctgcaggcat ggcgccggcc gcccctgtgc tggcaccgca gccttccgct gtcgcgccgc
    21661 cgcccacggc tgtgtcggtg gccgtgtcgc ccgcagcggc cggcatcatc gtgttcaagg
    21721 ccaaagggga gtcatgggtc gaagtcaccg atgccagggg tacggtcgtt ctgcgtcgta
    21781 ccctggctgc cggtgaagaa gccggggcat caggcgtcct gccgctggcg gctgtggtgg
    21841 gccgggtcga tgcgacgcag gtcgaggtgc ggggcaaggc ctttgacctg ggtgctgtga
    21901 gcaaagacaa cgttgcacgt tttgaggtga agtagtgaca gcatgttttc ccgtggagtc
    21961 ggccgcgccc aaggcgcgcg tgtcgtccca ggcgcgtgtg gtctggggct cgcgtgtggt
    22021 cacggtgggc ggcgatgcgc ccgtgcgtgt gcagtccatg accaacaccg acacggtgga
    22081 cgcgatcggc acggccatcc aggtcaagga gctggcgatt gccggatcag a
//
LOCUS       DAIDFG010000012        20453 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_12_length_20453_cov_18.025591, whole genome shotgun sequence.
ACCESSION   DAIDFG010000012 DAIDFG010000000
VERSION     DAIDFG010000012.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 20453)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20453)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20453
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12_length_20453_cov_18.025591"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3782 a         6662 c         6397 g         3612 t
ORIGIN      
        1 cccagcccat cgtggtggcc ggccgcatcc agctcgggct ggaccgcatg agccggcagg
       61 catgagcgcc gaacgcctgc tggccgcgct ggccgcgcgc gcacccgatg ccgtggtgct
      121 gcgcggcgag accgacacct ggcgcgccgc cgacgtgctg gacaccgtgg ccgcactggc
      181 catgcggctg tccggccacc gcgtgctggc cgtgctggcc gacaacagcc cggcctgggt
      241 catggccgac ctggccgcgc tgcgcaccgg caccgtgcac ctgcccctgc cgactttttt
      301 cagcccgacg caaatggcgc atgtgctgga acagaccggc accgacgtgc tgctgaccga
      361 ccagcccgaa agaatcctgg cgctgaacct cggctttgcc gcggcagggc aatggagcgg
      421 cctggtctgg atgcagcggc aatgccaggc catcgcgctg cccgccggaa ccgccaagat
      481 ctccttcacc tccggcagca ccggcacgcc caaaggcgct tgcctgagcg ccgccgggct
      541 gatggccacc gccgagggcg tggctgcgcg gctgggcgac ctgcctatca cgcagcacat
      601 ggccgtgctg ccgctggccc tgctgctgga aaacaccgcc ggcatttacg cgcccctgct
      661 gcgcggtgcc gaggttcact tgcccgccct gcaaaccctg ggctggcgcg gcatggccgg
      721 cttcgacccg gcggcgctgc agcagtcggt catggcaaac cacccgcaca gcctgatcct
      781 ggtgcccgaa ctgctcaagg cctggacgct gtttctgaca gctaccggcc agcgcgcccc
      841 ggtcgatctg tcgtttgtcg ccgtcggcgg cgcccgcgtg gacccggccc tgctggagcg
      901 cgcccgcgca ctgggcattc cggcctacca gggttacggc ctgaccgaat gcggctcggt
      961 cgtctgtatc aaccgccccg gcgatgacgg cccgggcgtc ggtcgcccgc tgccgcatgt
     1021 gcgcctgcgc atagcggaca acgaggtgca catcgccagc cccgcgttcc tcggctacgt
     1081 cggcgacgtg accaccagtc agccagccgg tgcggaaaac ttcgcgaccg gcgacctcgg
     1141 cgagctcgat gccaatggcc acctgctgct gtcgggccgg cgcaagaacc tgctgatcac
     1201 ctccttcggc cgcaacatct cgccggaatg ggtggagtcg gtgttgctgg cccagccggc
     1261 cattggtcag gcggcagtca tgggtgatgc ccgcccctgg ctcagcgccg tgctggtcgc
     1321 cagccccggc gccacgccgg cccagctgga cgcggccgtc gaacaggcca acaacacgct
     1381 gcccgactac gcccgcgtgg gcggctggat cgccggcccg cccttcacgc tgcacaacgg
     1441 ccaggccacc ggcaacggca ggccgctgcg cagcgccatt gccacccacc atgccgtcgc
     1501 cctggcggcg ctttacgaaa caaaggattc tcaagatgtc gttttatgag caactcaccg
     1561 cgtccaccca aaaagagcgc gaccagctgc tgaccgtgcc catcattgcc gactgcctgc
     1621 agggccgcgt caagctgccg gactaccacg cctttctcgg ccaggcctac caccatgtgc
     1681 gccacaccac cccgctgctg atgtccctgg gcggacgcct gcccgagcgg ctggcctggc
     1741 tgcgcccggc ggtggccgag tacatcgaag aagagatcgg ccatgaggaa tggatactga
     1801 acgacatccg cgcggccggt ggcgacgccg acgctgtgcg caacagccgg cccgagctgc
     1861 ccgccgaatt gatggtggct tatgcctacg acctggtgaa ccggggcaac cccgcgggct
     1921 ttttcggcat ggtgtttgtg ctcgaaggca ccagcgtcgc gctggccctg atggctgccg
     1981 accgcatcca gcaggggctg aaactgccgg acgccgcctt ttcctatctg cgttcgcacg
     2041 gcacgctgga ccaggagcac acaaaacatc tggctgaact gctggagcag atggcaccgg
     2101 aagaccagca ggacgtcgtg cgttatgccc gcgtgtttta ccgcctgtac ggcgacatct
     2161 tccgcgccct gtcgcacccc gaggccgccc catgcaactg aaccaggcgc gcattctgct
     2221 gaccggcgcc agtggtggcc ttggccggga cctcgcgcgc gaactggccg gtgccggcgc
     2281 cgccgtcctg ctggccggcc gcgacgcggt gcgactggcc gccatcaaag ccgagctggg
     2341 cgccagtgcc gacagtgtgg tcgccgacct caccacggca gaaggcatcg ccgcagcaaa
     2401 cgccgccgcc cgcgccttcg gtgtcaatgt gctgatcaac aacgccggcg tcggctcttt
     2461 tggtttgttc gaccagcagg cctggcccac cgtcgaacag gtgctggcca ccaacctcga
     2521 ggcaccgctg cgcctgacgc aggccctgct gccgtggctg aaggcgcagc cgaccgccgc
     2581 catcgtcaac gtcggctcca tgttcggcag cctgccctat cccggctttg ccgcttattc
     2641 cgccgccaag gcgggcctgc gcggtttttc gcaggcgctg cgccgcgaac tggccgacac
     2701 cccggtggct gtcatccata tcgcgccgcg cgtcatcgat acgccgctca acaccgaagc
     2761 cgtcaaccag ctcaaccgcg cgctgaagaa caacagcgac agcagccagg ccgtcgcgcg
     2821 gcagattgtc gcggccctgc gccacggcaa gggagaacac catttcggtt ttccggaacg
     2881 cctgtttgcc tggctcaacg gtttcgcccc ctcactgata gaccgcggcc tggccggcca
     2941 gctggccatc atcaagcaac acgccccctc ttctgaacag ccatgaaagg cctttccatg
     3001 caccccgttt cacgctccct cctctctgtc ctcatcctgc cccttctgct ttccttcgcc
     3061 ggctttgccg ccgcggccac cccggaagaa ctgatacgcc ccatccagga ccagtgggcc
     3121 gagatcaagt accgccagcc tgaaaaacag caggccgatc tgtaccacgc cctggcgcag
     3181 caggtgcaca agattgttga agccaacccc gccagcgccg aggcgctgat ctgggaaggc
     3241 atcgtggtct ccagcgaagc cggcgccaag ggcggcctgg gcgcgctgtc gctggccaag
     3301 gaggccaagc tgcgcttcga ggaagcgctg aagatcaacg acaaggcgct cgccggttca
     3361 gcctacacca gcctggccac cctgtacgcc caggtgccgg gctggccggt cggttttggc
     3421 aacaaggaca aggccgaaga gttgttcaag aagtcgctgg tcatcaaccc gaccggcatc
     3481 gacgcgaact tcttctacgg cgagtacctg ctcggccgcg accgccatgc cgaagcccgc
     3541 acccacctgg aagccgcact gaaggccccg ccacgcccgg gccgcgaact ggccgacagc
     3601 ggccgcaagc aggaaatcca ggcgttgctg gcgaaactga acaagtagat atgctctgct
     3661 tttgatagct gcttgcgcac gtcgtgtaag ggatggaggc tttttttata ctaaaagatg
     3721 catggctgca cttggcgcca caccggacgg tgaagcgggc gcggttggcg gcttgcaccg
     3781 ggacgtgtcc gcttgcgtga cggcataacc ctcaatttca caggctgacg gacggggcct
     3841 taccgacgcg agcaggccag ataaacagcc ccgccgctgc gacaccggct acgagcaaac
     3901 cgaggtcggg tttgacgtcc aggaggaagg cggcctgact gcccaccagg gaccaaagaa
     3961 tgggcacagc gaggactgtg cgcaggcgcg tgcccgacgc gagcgcgagc acaccgatcg
     4021 tgaagatggt ggtggggcac ggcagaccaa aggtgggcag ctccggatac ccatgccctg
     4081 ccagcgtgga ccagacggga taaacgacga gtgcgaaggc gagcagggct accccgagac
     4141 ccgtccgaaa gctcctgtca aaagcaaact cgaatcggcg gcagatcacg ccatgccagg
     4201 caaacagaag acctccggcg atggacagcg cgccgaaccc gtaggccaca ggattgatgc
     4261 gcgcgaaaaa cgccaggtgg taggcggcgc caagccaaac ccagagcaaa gccagaatgg
     4321 ctgacacggc cgcgccggac caggggcgcc gaagcgcaat gaagacgatg gcgagcaccg
     4381 caagcaggac aagaagaacc tgtgccggcc agacggtgct gttgtacagc cggaagacgc
     4441 caaaaaactg ctcaatggtg aagggcagct gcatgggggc tcctaacggt tagcgtttga
     4501 ggtaaggagc gcggagccag cttgccggag gagcgtccct cttgatcgat tggttgggcg
     4561 tcatttgatc gacaccttta gatatcgccg tagcgttcgg agagcacgat tatttcggcg
     4621 aagatcgttt agttttgtac gacttcggcc agcgagaatg cgctttaatt ccgtgttgta
     4681 ctggaacggc actgtggcca caagctgcgc gtagatgtga tcgaggtcgg gaagaaaaga
     4741 gctgggaatg ccgatgcctt tggtgcctcg tgggtatcgt gaagcagtaa ggtagcgttg
     4801 gtcgaagtag aagtactccg gaatttctat atcttctgag ttggggacct tattccccac
     4861 catccgcgcg agtttcgcca tagcatgccc tgcggcctca ggatcaaatt tcctgtccca
     4921 gtacaccaac gttgctttaa gcagtagttc aagcgccatc accgcacaat aggcagcggg
     4981 gcgtaaaaga tcggactgat acaagcgacg gcttgcgagg tagaaatcgg tagcccggtt
     5041 ctgccatgca acgtagctag tttcgtgggg tgagaatgtg gtcatggcgc ccaattttga
     5101 tgtatcccgc aaaaccgcag aatcacatgg cgcccggcaa aaaaaatggc tgaagtattt
     5161 ctaaattctt catacttttt aaagcttacc ccagcgacag gcgcaagtga acagcgctgt
     5221 tgaaaacggc caaatcgccg accgtgcaca gggccagccc tgaacccgct atatctttta
     5281 tagctgcttg tgcccgtcag acaatggctg gaggccgatt actccattca accgagtgcc
     5341 agcccggcca catccacctc aacccgcgct gaccgcgctc acgcagcgca ggcgggccgg
     5401 ggtgttccgg ttcgacgatt tattgcggct cgatcttcgc gtcgcggatc gccttggccc
     5461 acttggcggt ttccagcttg ctgcgctggg ccagggcttc tgatgatccg ggctcgacca
     5521 ggaagccgat cggtgcgaat ttttcctgca ggtccttgct ctgtgccgcg gcgttgatgg
     5581 cctggttcag tttggcaatg atgtcgggcg gcgtgccggc cggtgcgaac atggcgaagt
     5641 aggcgatcag ttcgtagtcc ttcaggccca gggcttcgtt gaccgtcggc agttcaggca
     5701 ccgctgggga gcgtttgctt gacgtcaccg cgaggccgcg gaccttgccg cccttgacct
     5761 gcgggagcat gacggcaaag tcggcggtga acatcatcac ctggccgccg atgaggtcgg
     5821 tcatggccgc agggccgctt ttgtaaggca cgttggtcat ctggatgccc accatgccgg
     5881 ccatcatttc ggacgacacc agttgcgagg tgctggcgct ggcaaaggtg acctggcccg
     5941 gtttggcctt ggcgatgtcc acgaattcgc ggaggttttt ggccggcacg tcgttgttga
     6001 cggccacgat cagcggcacc gaccccatgt agcccagcgg cgcaaaggcc gtgtcctggt
     6061 cgtaggggag ctttttcatc aggctcttga gcgcagcgtt ggtgctgttg gtcccgatca
     6121 ggatggtgta gccgtcgggt gcagacttgg cgacagcatc ggcccccagc atgccgttga
     6181 ccccggcctt gttctcgacc acgatggtct gccccagcgt ttccgacatc ttggccgcga
     6241 aagcacgccc gatctggtcg gtggcgctgc ccgcggcaaa gggcacgatg gccttgatcg
     6301 gcttgttcgg atacgcctgg ccgaacgcgg cgaaagacaa actggccgca gccaggaaaa
     6361 gaatcgactt gcacttcatg ttgatgtctc ctgtttaaaa attgttgaat atatccagca
     6421 aaccgtctag cttattcgat ggccatgccg agaatggcgg agctcaggca cttaccatgg
     6481 gcgtccagcg ccagggaccg ggtcacaccc ccgcccagcg cgtcgtgcag cacgaagttc
     6541 agggcgccca gatgcggcac cacataacgc tcgacctcgc ccttgacggt gtccgcgtaa
     6601 tacgcacgca cccgttccgc ggtgacggcg cgctcgatcg ccgggaagtc cttgaggtcg
     6661 taggcgatga cggaaatgat ggaccggttt cccttgtcgc cggtacgggt gtgggcaatg
     6721 tcgcgcaata tcatttttca aatctccatc atggtgactg aggtctgaac cagattgcgc
     6781 ggcaccaggg tggacgccgc ggcgatcacc tcgcggaccg acttggtgac gccgcccccc
     6841 ccggccggcc cgttaaggta catggcctcg acctcgttgg ccacccgctc ggcctctttc
     6901 agggtcggaa cacgcagggc cacacgcgcg cgcacttcgt aaggcgtgtg gcccgcggac
     6961 agggtctcgc catgcatggc gttgaccccg atgagttcat aacggctgtc gagcgcatgg
     7021 atgtccgaag tctccaggcg ctgcttcagc acgtccagcg ccagccggcc ccgcgcctcg
     7081 gcgcccaggc cggcatagga aatctgccct tccccgatga aaccgtcgcg gtaacccacg
     7141 gtcactttca gtgtcgccgg gcgctgatgg ccgttggccc cttcgatacg cacgcggtca
     7201 gggcctacct gggtcagcgt gacatttgaa aagtccgcca ccacatcagg ctgcaggtag
     7261 ctggcggggt tttcaacttc atacagaagc tgcgccgcgc aggtggcggt ggtgacgcag
     7321 ccgcccgaac cggccacctt ggtgatgacg gcgctcccgt cggccgacac ttccgccagc
     7381 ggaaaaccca ggttggcgag gtcgggcaca tcgcggtaac cagggtcggc aaaatacccg
     7441 ccggtgacct gcccggcaca ttccagaaga tggcccacca ccgtgccctg acccatcttc
     7501 tgccagtccg acatggacca gtcgaacgca tggatgagcg gcgcaagaaa cagggccggg
     7561 tcggcaacac gcccggtgat gaccacgtcc gcgcccagac gaagcgcccg caccagcgcc
     7621 tcggcgccga gataagcatt cgccgacaac acagcgctgc ccagggactg cagcggtgca
     7681 ccgctttcca gcagggtcag gtcctggtcc tgcaggccgg gcaacacgtc gtcgccctgc
     7741 accaccgcca ccttcaggcg gtccagcccc aactcgcgcg cgatgcggat gacttcctga
     7801 ccggccgcct gcgggttggc ggcgcccatg ttggtgatga ttttcacgcc cctgccagcg
     7861 caggccagca gcaccgcctc catgcggtcc cgcagcagcg ggtcgaagcc cagacgcgcg
     7921 tccttgctgc gctcgagctg ggccagggcg atggtgcgct cggccaggca ctcgaacacc
     7981 aggtagtcga ggtcgccctt ttccgccagc tccacggcgg ggggaatgcg gtcgcccgag
     8041 tagccggcgc cggcgccgat gcggatgatc ttcatgaagg ggctttcaca gggaacgcga
     8101 tgttgaagca aggagtgacg gggcacacca gacggactat ggagcaagcc ggaccgcctg
     8161 tgaattcgaa attccggatg agactcaaag gaaaaccgga tcaatccgga tccatcggcc
     8221 cggcgggccg aggacatcct tgggtatcat ccgcacggat gaacctgtcc acacgccaac
     8281 tgcgtgcttt cctggcactg gcggacctgc gcagcttcac acgcgccgcc gagcagatgc
     8341 atttgtccca gcccgccttc agctccctga ttcgttcgct ggaagacacg ctggccgcca
     8401 ggctgtttga ccgcgacacg cgcaaagtcg acctgaccgc agccggccgg cgctttgaac
     8461 gcgccgcgcg cgaggtggtg tcgcacttcg acaatgtcat gggcgcggtc tgccgcgacc
     8521 tcagcatcgt ggaccgcgtg gcggtggccg gcatcccctc gctgtgcgcc cgcttcctgc
     8581 cggtggtggt ggccgactac cggcgcaaac acccggccat cgacatcgag gtgatcgatg
     8641 cgctgcccca accctgtgtg gacctggtgc gggaaggcaa ggtggacctg gccctgtcca
     8701 ccatcgacgc gaaagatgcc gacatccaca aggaactgct gtgcctggac cgcttctacg
     8761 tcgtctgccg gaaagaccat gtgctcgccg gcaaggcggc catcacgatt gaagagctgg
     8821 tgcagttccc gttcattcac ttcacaacga ccacccgcct gcgccaccag atcgatgcgg
     8881 ccttgcgacc gcacaagccc aacacggtga tggaggtcga gcgcttggag gcggtgcgcg
     8941 gcctggtgga aagcggggtc ggcatcagcc tggtgcccag cctgacgctg tcgcacttca
     9001 gcagcagcca gctcgccatc aagcagctgg ccgcacccgg cattgttcgc gaggtgtttc
     9061 tgctgcgggc gcagggacgc acgccctccg ccgcggccga tgccctggcc gaaccgatcc
     9121 ggcggcagat gcagtcgctg ctgcagcctg gcagccttga actcgatcat gaaaagctgg
     9181 ctgccctgat gccggacctg aaggactagg cccactgcct ctttcttgag caagcccggc
     9241 agaaccagca ctggcgcggc atttcggggt tgttcacccc cttatccaca gcctaggcaa
     9301 cagggcgtgg gggtaacttc cggtgggtgg tcgccgaaac cggccatcaa acgctaaacg
     9361 ccgtcgggga gcctggaggc catcatcttc ctgcggtcca gcttgcgccc gtccaccatg
     9421 aagcttccgt cgcccacggc atgaaccatg cgcttctcct tgcgcgtggt atcgacccag
     9481 gcggccaccg cctccagcac gacgatgttg tgctttttga cgatgtcgat gaccgtgcat
     9541 tcgatgttgg cgaggcactc tttgatcagg ggcgccctga cgacttttcc cggcacaggg
     9601 gtgagcttga actggccaaa cttgtcggtg tctttcccgg agcaggtccc tatgccgacc
     9661 accttgtcca gcatgtccac cgtgggaatg gcaatgacgc actccctgct tttccgcaag
     9721 gccgcgtaag agtgattcca tggtcccgtg gtgatggcaa acaccggcgt gaaatccatc
     9781 accatggtcc aggagatcgt catgatgttg tctttcttcc cgtcatgggt cgtcaccagc
     9841 accacaggcc ctggttcgat cagggtgaag gccttgttca ggtccagctc acgcatgaca
     9901 acacctctcg atattcggat ggcagggcct gaactgtagt ccagcgcaag accatgggac
     9961 gccgccgtcc gtgccgagcc tggggttcag tcgttcagga cagccagaaa ctgccgcagc
    10021 tcgggcgtct gggggcggtt gaaaatttcg tccggtgtgc ccgcttcgtg aatcagcccc
    10081 tggtgcatga acaccacgcg gtcgctcacc ttgcgggcaa aggccatttc atgggtgacc
    10141 aggatcagcg tcatgccctc gttggccagc atttccacca cctgcagcac ctcgcccacc
    10201 agttccgggt ccagggcgga ggtgatctcg tcgcacagca gcaccgtggg gtccatggcc
    10261 agcgcgcggg caatggccac gcgctgctgc tgtccgcccg agagttgatc gggcatgcgg
    10321 tcaaacaggc tgcccagtcc cacgcgctcc agcagcgcct gggcacgcgt gcgcgcctct
    10381 tccttcggca agcccttgac cagcgtaggc gccagcatca cgttgcgccc ggcgctcagg
    10441 tgggggaaga ggttgaagct ctggaaaatc atgccgacct gctggcgcag ctcgcgcatg
    10501 gcgttggtat cgccatgttg cagggcctgg ccatccacgg ccaggctgcc gcgctcgaag
    10561 gtctccagcc cgttgatgca acgcagcaag gtgctcttgc ccgagccgct cttgccgatg
    10621 atgcagacca cttccttgcg cccgacctgc aggtcgatgc ccttgagcac ttcggtggcg
    10681 ccaaaactct tgtgcaggcc acggatgtcg accagcgcaa acgggggggt ggatgatggt
    10741 tgggtcataa atcaggccag gcggcggttt tccagccgct ggctccacca cgacagcggg
    10801 taacacaggg caaagtacag gaggcccaca aaggtgtagg ccagaaaggg ttggtaggtg
    10861 gcgttggaga tcatctggcc ggccttggtc agctcgacaa aaccgatgac cgaggccagc
    10921 gccgtgccct tgatgatctg caccagaaag cccaccgtgg gcgcaatggc cacacgcagc
    10981 gcctgcggca ggatcacatg gcgcatctgc tgcgtgaagt tcagcgccag ggtggtggag
    11041 gcttcccact ggcctttcgg gatggcgttg acacaaccgc gccagatttc cgtcaggtag
    11101 gcgctcgcat acagggtgag gcaaatcgcc gccgacacca gcggcgaggt gtccatgccc
    11161 agcagggcca ggccgaagta caccagaaac agctgcatca gcagcggtgt gccctgaaaa
    11221 atctgcacat accaggccac cgccgtggcc gcaccgcgca ccctggcaaa ccgcagcaac
    11281 agcaggccca aaccgaccaa cccgccaccg acaaaggcaa tcagcgagag cgccaccgtc
    11341 cagcgcgcgg cgcccagcag aaagctgaag atgtcccaga gagaaaactg aaccatggtg
    11401 gctcccgtta acgactggaa aagatgaagc gcggccccac ccagtacagc aactgccgca
    11461 cgccaatcgc cagcagcagg tagatcgcgg tgacgatgat gtaagactca aaactgcgga
    11521 aagtccggct ggaaatcagg ttggccgcct gcgagagttc ttccgccgag atctgcccgc
    11581 acaccgccga ccccagcatc acgatgatga tctggctcac cagcgcgggc cagatgcgcg
    11641 ccagtgccgg cggcaacacc acgcgcgaga aaacctgcca gggcgtcagc gccaggctca
    11701 tggcggcttc cagctgcccc ctgggtgtgg tctgcaggcc ggcgcgcacg atctcgcacg
    11761 aataggcgcc caggttgagc accatggcca gcacacacgc cacctcgacc gacagccgca
    11821 gtcccagcga gggcagtccg aaaaaaatga aaaacagctg gatgatgaag ggcgtgttgc
    11881 gcatcagttc cacataggca cccacccccc gtttcaggaa cgtgccgcca tgcagccggg
    11941 cccaggcaca cgccgtgccc accacgacac ccagcaccgc cgaaacggcg gtgagcgcca
    12001 ccgtcaggcc caacccgcgc agcagcatgg gccactgggc cagcacggtg agaaagttga
    12061 attcaatcat gatgagtcag gaatgctgcc gaagtgatgc cccgctccgt tcgggctgag
    12121 cttgtcgaag cctgtcctga actggacgaa gggcccttcg acaggctcag ggcgaacgga
    12181 tgttgagtaa aggtttggaa gcgctcaaca accttaatca ggcaacacgc cggtgccgcg
    12241 gcccagccat ttttgcgaat actgctccag cgtgccatcc gccttggcgg cgcgcaggat
    12301 ctcgttgacc ttgttcagca acgcggtttc acccttgcgc acgccgatga agttgggcga
    12361 gtctttcagc agcagcttgt actcggcctg cacctggggg ttcttctgca cggcagccgc
    12421 ggccacggcg gcgccggtgg ccagcaactg ggtttgctgc gagatgaagg cggccacggt
    12481 ggtggagtcg tcctcgaagc gctggatttt cagcgtgggc ggggccactt tctgcagcac
    12541 gtcgtcctgg atggtgccgc gggtcacggc aatgctcttg ccggccaggt cgtcaaaact
    12601 cttgatgctc agcgccttgg ggccgaacac ggcctggaag aacggggcgt aggccacggt
    12661 gaagtcgatc actttctcgc gctcggggtt cttgcccagg gccgagacga tcaggtcgat
    12721 cttcttggtc tgcagatagg gaatgcggtt gggtgtgctg accggcacaa actcggcctt
    12781 cacacccagc ttgtcggcga tcagctgcgc tgtggccacg tccacgccgg tgagtttgaa
    12841 gtccgggccc atgtagccgt aaggcggaaa gtcggaggcg acgcccaggg caatcgattt
    12901 cttggccagc acggccgaga gggattcatc ggccgcgtgg gccggcgcgg ccacggccag
    12961 gacggcgctg gcaaccaggc cagccagggt ggagagtgct ttgaatttca tggaagggtt
    13021 cctgtgaaga tgagggggag acaccaactc tttcgcaaaa tgtgtgccac ctgttttgca
    13081 tggcattgcc cttgcaaaca gatgacggac aatatgaaat atgtgtttct cgaacataaa
    13141 ttcatgaaaa atatgtttca tagtggtgcg catgctctct tttgggcgta tccgcaccaa
    13201 aacctctcag ccgccctgcc atgaccaagt ccaaaaccat caccctgcgc gaacggattc
    13261 aggttcagta cgctgcgctg tccctggtcg accgcaaact ggccgacgtg gtgctggccc
    13321 gtgaaaaaga cctgctggcc tacagcgcca ccgagctggc ggcgctggcc ggtgtctcca
    13381 aggccagcac ggcgcgtttt ttcaagcgcc tgggctttgc cgacttccag gccttccggc
    13441 agcaccagcg caatggcaca ccccagccct cgccgctgca ccgcatggcg cagcagggca
    13501 ccagcgccag cccgctgcaa caccacctgg cgcaagacgc ccggcagttg caggccctgg
    13561 ccgacagcgt gaccgaccag ggcgtgcagc aggcagccag gctgttggca ggcgcgcgcc
    13621 gcgtctgggt ggtgggttac cgcaacggct acatggcggc gtcttatgcg cgggcgctgc
    13681 tgtcgcagat gcgcgccgag gtccatctgc tcaacgaggg cgccggtgaa gaggctgaac
    13741 tgctggcgga aatccagcca aatgacgtgc tgctggccat ggactttcgc cgccgcaccc
    13801 accgcctgtc ccaggtggtg ggcatcgcct gtgatgccgg tgccgcactg gtgctggtca
    13861 gcgatgcccg cgtgtcggcg ctggcagcgc gggcgcaggc cctgttcatc tgcccgccgc
    13921 tggacgagcc gatttttgac tcctatgtga gcgccatcag cctcatcaac tacctggcca
    13981 ccgccaccct ggcccagttg cccaaaaagg cacgcacgcg catggccgcc atcgaacagg
    14041 tccatgcgca actggccgac ctcgaacctc ctctctgatc ctctctgaac catcccacct
    14101 catgaacgcc ttcacccaac accctttcac gctgctgccc aatccccgct ttgaacccgg
    14161 cgagccctat ggtgcgcacc gcagcaccct gctcagcacg gcggccctgg agcaagccag
    14221 cgccgtcatc acctcctggt ccggctacgc ggtcacgccc ttgtgttccc tgtccagcct
    14281 cgcccaatcc gcaggcgtgg ccagcgtcca ctacaaggac gaaggctcgc gctttggtct
    14341 gggcagcttc aaggcgctgg gcggggccta cgccgtggcg cgcctgctgt gccgcgaact
    14401 cggccagcgc ctgggccgca gtgtcagcca cacgcagttg caaagcgagc ccgcgctgcg
    14461 ccaggcctgt gccgacatca ccgtcacctg cgccaccgac ggcaaccacg gccgctcggt
    14521 tgcctggggc gcgcagatgc tgggctgccg ctgtgtcatc tacatccatg ccactgtcag
    14581 cgaggggcgc aaagcagcga ttgcccgcta cggcgcgcag gtggtgcgca ccagcggcaa
    14641 ttacgacgat gcggtgcaac aggccgaccg cgatgccagg gccaatggct ggtttgtgat
    14701 ctccgacacc tcctaccccg gctacatgga cattccgcgc gacgtgatgc agggctacca
    14761 gctcatggtg cacgaggccg tcagcgccct gccgcagtgg cccacccacg tcttcgttca
    14821 ggcgggcgtg ggtggttttg cggcggccgt gtgtgcgtat ttctgggagc gcgacaccgc
    14881 gcagcgcccg gtgtttgtgg tggtggaacc cgaacgtgcc gactgcctgc tgcaaagcgc
    14941 gcgtgccggc gccgtggtgg cagtcaaggg cgatctggac accttgatgg ccggcctggc
    15001 ctgcggcgag gtctccctgc tggcctggga cttcctggct accggcgcca atgcgttttg
    15061 caccatcccc gatgccgccg ctgtcgatgt catgcgcctg ctggccagcc cgcagacggg
    15121 tgacccggtc atcgtcgccg gtgagtctgc cgtggcgggc ctggccgctg cgctgctcgc
    15181 cgcgcaagac ctcgccgccc gcgcaacgct gggcctgggg ccggacagcc gcgtgttgct
    15241 gttcggcagc gagagcgaca ccgaccccgc cctgtaccag caactggtgg gccgcagcgc
    15301 cgcccaggtg ctgcaggacg gagcggccgc atgagcgccc cacagatcaa cggcccgcgc
    15361 ctgctggaga gcctgcaggc gctggggcag atcggcgcgc tgcccggtgg cggcgtgaat
    15421 cgcctggcct tgaccgatgc cgaccgcgcg ggccgcgact ggacggcggc gcgcatgcgt
    15481 gcgctgggca tgcgcgtgga ggtggatgcg attggcaaca tcattgccac cttccccggc
    15541 accgaggatg gccccccggt gatgatgggc tcacacatcg acacggtgcg caccggcggc
    15601 ttgtacgacg gcaactacgg tgtgctggcc ggtctggaag tggtggccac gttgcgcgag
    15661 gctggcctgc caacccgccg accgctggcc gtggccgtgt tcaccaacga ggaaggcgcg
    15721 cgtttccagc ccgacatgat gggcagcctg gtctacgtgg gtggccagcc gctgcaggag
    15781 gcgctggcca cctgtgatgc cgatggcgtg tcggtgcagg ccgagctgca gcgcatcggt
    15841 taccagggca cggccccggt gggtatgccg gtggtggaca gctttgtgga gctgcacatc
    15901 gaacaaggcc cggtgctgga acagatgggc ttgcagatcg gcgtggtcga aggtgtgcaa
    15961 ggcatctcgt ggaccgagtt cacggtggaa ggccagtcca accacgccgg caccaccccc
    16021 atggccctgc gccacgacgc cgcgctggtg gctgcgcaag tggcggtgtt cgtgcgtgaa
    16081 ctgagtagac gcatgggcgg ccgccagctg gccaccgtag gcgcgctgcg gctcagcccg
    16141 aacctggtca acgtcatcgc gcaaaaagcc gtgttcaccg tggacctgcg caacaccgac
    16201 agcgccgcgc tggcacgggc cgaaacggac ctgcatgcct atgtacaaca agtggccgag
    16261 gccgaaggcg tgcgcatcag ctcgcgcgca ctggcccgtt ttgagccggt ggacttcgac
    16321 cccgcggtgg tcaacctggt ggagcagcag gcgcaggccc tgggctgccg ccaccagcgc
    16381 atgcccagcg gcgccgggca cgacgcgcag atgctggccc gcatttgccc cagcgccatg
    16441 atttttgtgc ccagcgcggg cggcctgagc cacaacgtgc gcgagcacac cgaacctgcc
    16501 gacctgcagc gcggcgcaca gctgctgctg caagtggtgc tggcgctggc caatcgcccc
    16561 gcatgatgcc ggcaaccatg ccctcctccc tccgttcggg ctgagcctgt cgaagcctgc
    16621 cttccatcaa cttttacctg tccacttatg ccacgccatc tcaacgtcgc cctcggccag
    16681 ctcggcccca tccagcgcgc cgactcgcgc tcccaggtgg tggcccgcct gtgtgatctc
    16741 atgcgccagg cccacgccgt gggcgcgcag ctcattgtgt ttcccgaact ggcactgacc
    16801 acgtttttcc cgcgctggta catcgaggac gaggccgaga tcaacagctt cttcgagcag
    16861 tccatgccca acgccgccac gcagccgctg ttcgacctgt cgcgccaact cggtgtgggc
    16921 ttctacctgg gttacgccga gctggcgcag gaggccaccg gtgcggtgcg ctacaacacc
    16981 tccatcctgg tggaccgcag cggccacatc gtggccaagt accgcaaggt gcacctgccg
    17041 ggccacaagg agcacgaacc ctggcggcgc ttccagcacc tggaaaagcg ctacttcacc
    17101 cccggccccg gctggggcgt gacgcaggcc tttggcggcg ttttgggcat ggccctgtgc
    17161 aacgacaggc gctgggccga gacctaccgc gtgatgggcc tgcaaggtgt ggagatggtg
    17221 ctgatcggct acaacacccc ggtgcacaac gcgccggcgc cggcgcatga cgacctgtcg
    17281 ctgttccaga accagctgtc catgcaagcc ggcgcctacc agaacggcac ctgggtggtg
    17341 ggcgtggcca aggccggtct ggaagaaggc gtggacagca taggcggcag ctgcatcgtc
    17401 gccccgtccg gcgaggtggt ggcgcgctgc ctgaccaaag acgacgaaat ggccatcgcc
    17461 cgctgcgacc tggacttttg cgccgtctac aaacgcacca ccttcaactt cgacctgcac
    17521 cgccagccgc acgcctacgg cctgatcgta gagcgcaagg gcgaaacgct ggccgccgac
    17581 ggctcaccgc tgcacctgcc tgtttcttga gcatgcccgg catcgccagc actggcgcgg
    17641 catttccggg ttgttcaggt tcttatccac agcctaggca acagggcgtg ggggtaactt
    17701 tgggggtggt cgccggggtg cggcccgcac ctcatgcctg cttggctggc caggttgaat
    17761 cagctgagtt gctcttgttt tgatagctgc ttgcgctttc tgcataaggg ctggatgcct
    17821 gtttgcatct caaagagctg ccgatgcgga gtgtcgagca acatgaaggc ctagttcgtg
    17881 gtcggaatca gactggaagc ggccattcgg atgtttgccc gatcgaccgc tatgggccca
    17941 atgcagtcct acggtcgtca agagccgatg actgcttcgt agcgcaacga gtcggtgcca
    18001 acgcccgctt cctggaacct caattgaaac agcttgcatg cagtttggcg acagcaacgg
    18061 cgtcgtgccc tttgaccggt gaacgatgcc gtgcggctgt gttgctgggt tggcatccgc
    18121 accactcgca acggttttaa caccttatcc actgcacagc ttagaaatgt agattgtcaa
    18181 tttggctgaa gtcaaaacga cggataaagc gcattgcacc gatgcgcatg gcagctttta
    18241 ccttacagaa gatgagtgga gcctggagct gaatcaagtc gtttgccgta tcaccgtctg
    18301 acttgaagat ttccggtgtt catattcatc atcaatgcga attgtcaacg actacccaac
    18361 gccgattcct atactttgcc catcgggctt attttcacgc gctttgaaag tgtgcgcaat
    18421 aagtattgat agagggttta gtttttcttc gaaaatggag aatggcatga aaatgacact
    18481 tatttcagcg acgttagcga tggctgtcgg cctcaccttt ggaggcttgg ctttggctgc
    18541 tggtgcaggt accaacgctg cggagcccac atacaaagag ggtgaaacga tattcgtcga
    18601 gaccggggta gaagctcctg accgcactcc aacttggtac gcgtccatcg tggccaagcc
    18661 ttatgcgcct gtgtttgaga tgctggctac ccagggcaca caggggcggt actaccctta
    18721 tacctatccc gtcacactga aggacgtgat ccggtttcat ggtcacgact gcgaggggac
    18781 taccacggcg gccagctctg cgtggatggc tttcaagatc ctgtttccgg atggcatcat
    18841 tgatcgcagc cttttgtggg gcatcagcgg cgagtcgccc tgctggtcgg acgctgtggc
    18901 atttctcagt ggtactcgtc tgcagtatgg caaccttggg ttttttaagg acaaacgtta
    18961 tagccacgcc atcgtcctgt accgcgaaga cacaaaaatt gccgtactag ctacctggaa
    19021 ggaaggtgtc aacaacattc cgggcgagcc ggtcatgttg cctggcaaga tcacctggaa
    19081 acccaaagtc accatgcagg acgtcgaggc gctcaaggca gtcgtgaaat tggcgggcgg
    19141 caacccgaca ccctaccaag tggacttgat gcgttatcaa caatgggtgc atgtcaacga
    19201 catactcgat cgaccgcttg ccgagagtta ccaagcgcag gtcatcgaaa acttcaagtg
    19261 ggaagactgg atcgacccga gcaaagtcgt tgtcaaccca catcaacgca gcgatacccg
    19321 cctgaagaac cacccctacc gaggtcgccc ggtggaagga cctcgctgac aatccactgt
    19381 caagttgttg gcagttccgg tactggagga atctgcccga tacgcttggc tgcaaactga
    19441 tacataacga gtccttgccg gcagttgctg ccgacgccaa tgtgaccagt tgtgccggaa
    19501 ctcagggatc acccaaagta cggggaagtc tgaatgtatt tgcgagttgc agaagcaaag
    19561 tcactggcca gtcgaaattg acgtacgcgc caacagtcac ggggcaaagt cagcatgtgc
    19621 ccgctttcgg gcgacctgtt tcaagtgttg aatgactcat atgggtctga agcagacggt
    19681 caattggtgt gacgctaacg gccgcttctg gccgaaaacg gcttttcatt tgagtccgac
    19741 gaccggctgc attagccgca ttgcagccac tccatcggac tgatcccctc aagaactacg
    19801 cgccaccccg cacaccagat cacccggctc attggccagc accgcacgtt cgttcttggc
    19861 ctgcgcgact tccatgtcgc gcggcaggac cacgccgttt ttcacggcca gcacctcggc
    19921 catcacgctg acggcaattt cggggggcgt cttgctgccg atgtagatgc cgatggggcc
    19981 gcgcaagcgg gccagcgcgg cttcgttgag gccgaagtgc tcggccatac gctgatggcg
    20041 cgcgtcgttg ttgcggcgcg agccgatggc gcctacgtaa aacgcatcgg tggccagcgc
    20101 ttcgagcagc gccaggtcgt cgagcttggg gtcgtgggtg agcgcgatca cgcagctgcg
    20161 ccggtcgggc ttgaaggcga ccaccacgtc gtccggcatg tcgctgagca ctttggcgcc
    20221 cgcaaccgac caggcgctgc ggtactcctc gcgcgggtcg cacacggtga cggcaaaacc
    20281 gctgaacagg gccatggtcg cgaggtattc ggtgagctgg cccgcgccta tgagcagcat
    20341 gcggtactcg ggtccgaagg tgttgaccag ttcgacggac gagacactga gctcggccgg
    20401 ggccctggcc gtgaccaggg tgaccgcgcc gtcggtcagg cgcaccgtgc gct
//
LOCUS       DAIDFG010000013        19808 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_13_length_19808_cov_18.619197, whole genome shotgun sequence.
ACCESSION   DAIDFG010000013 DAIDFG010000000
VERSION     DAIDFG010000013.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 19808)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19808)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19808
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13_length_19808_cov_18.619197"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3381 a         6336 c         6601 g         3490 t
ORIGIN      
        1 aagatcgagg cagcggtgtc cgacgagctg gtggaccgcg tcatcgaagc cgtcgagggc
       61 gcagcccgta ccggcaagat cggcgacggc aaggtcttcg tctacaacct cgagcaggtc
      121 gtgcgtatcc gcaccggtga aaccggcaag gaagcgctct aagcctcaac cctcaaaaag
      181 agaaatagac atgaaaaaac tacttgcctc cctggctctg ggactgagct tgctgacttc
      241 cggcgccgcc gtgctggcgc aggcgccagc ggctgccccc gcggcgtctg aagccgttgc
      301 accggcggct gctcctgccg cagtggctgc accggctgcg gccgaagcag cggcgcctgc
      361 cgcacctgtt gccgtgccca acaagggcga taccgcctgg atgatggtct cgaccatgct
      421 ggtgatcctg atgaccatcc ccggtctggc cctgttctat ggcggactgg tccgcagcaa
      481 gaacatgctg tctgtgctca tgcaggtcat ggtcaccttc tcgatgatca ccgtgttgtg
      541 gttcatctac ggctacagcc tggcttttac cgagggcaat gcgttcttcg gcggctttga
      601 ccggctgttc atgaagggtg tctgggacaa cgttgcgggt accttcgcca acggcgcaac
      661 cttcagcaag ggggttgtga tacccgagat catcttcgca gccttccagg ccaccttcgc
      721 cggcatcacc tgcgcactga tcgtcggcgc ctttgccgaa cgcatgaagt tttccgccgt
      781 gctgatgttc atggtcatct ggttcacctt cagctacgca ccgatggcgc acatggtgtg
      841 gttctggatg ggccctgacg cctacaccgc caaggaagtt gttgacgaga tgacctccaa
      901 agccggttac atctggcagt cgggtgcgct tgatttcgcc ggcggtaccg tcgtgcacat
      961 caacgccgct gtcgccggcc tggtcggtgc ctacatggtg ggcaaacgca ttggttacgg
     1021 caaggaagcc atggcgccgc acagcctgac actgaccatg gtcgggtccg ccctgctgtg
     1081 ggtgggctgg ttcggtttca acgccggttc cgccctggaa gccaacggct ttgccgccct
     1141 ggccttcatc aacaccctgg gtgccacggc agccgcggtg ctggcctggt gtgtcggtga
     1201 agcgctgatg cgcggcaagg cgtcgatgct gggtgcagcc tccggcgcag tcgctggtct
     1261 ggtggcgatc acgccggcag ccggcaacgt cggcatcggc ggcggcctga tcattggtct
     1321 ggtggctggt tttgcctgcc tctggggtgt caatggcctg aagaaaatgc tgggtgcgga
     1381 cgactcgctc gacgtgttcg gcgtgcacgg catcggcggt atcctcggcg cgctgctgac
     1441 cggcgtgttc aactccccaa gcctgggtgg ccccggctac gtggcagact gggtcacggc
     1501 caccatggtg acggcggcgg attactccat cgttgcccag gtctggatcc agctcaaggc
     1561 tgtgttgatc acggtggtct ggtccggtgt ggtttccttc atcgcctaca aggtggtgga
     1621 cctgaccatc ggtctgcgtg tcagcgaaga agacgagcgc gaaggcctcg acatcacgtc
     1681 gcacggcgaa accgcctaca gccgctgacc ggcacaagcc gtggccgtct ccgggtggcc
     1741 acggcggctt gaatgaattt gggataaata gcgagagtct cctttggatg ttgagcccgc
     1801 aagaactccg gttctggcgg gctttttttt tgcctgggcg gtttgtccac cgtgcacaat
     1861 gtcggcagga gacttgccca tgaccgagcc catcaccttg tccagcgcca gcctgcgttg
     1921 tgacatcaag cccgacctgg gcggttgcat caccggcctc tggctcggcg acctgcctgt
     1981 gctgcgttcg acgcccgccg gcgagctgat gtcggtgcgc ctggccggca gttatccgct
     2041 ggtgccgttt tccaaccgtg tcggccatgc cacgctgaag tggcagggca ccagccatcc
     2101 gctggtgcag aacaacgggc cggagccgca tgccatccat ggcctgggct ggcagcggcc
     2161 ctggcaggtg ctcgaccagg acgagcagct gctgatgctc gctttcgaac acagcgccga
     2221 cgccgcctgg ccgtttgcct ttgacgcctc gcagactttt cgcctcagtg gcaacaccct
     2281 cgaactcacg ctgagcatga ccaaccagtc ggccacaccg gcgccggccg gtctgggctg
     2341 gcacccctac tttgtcaagc gcaaccgcag ccgtattgcg tttgaagcga ccgggcgcag
     2401 ggagatgaac gaggaaaaac tgccgacgca ccgcctgccg gcgcacggac tcgacgtgga
     2461 ctgcgccgcg ctcgatgtgg accactgctt tgacggctgg aacggcgtgg tgcacctgcg
     2521 tgatgaaaaa atgcacacac gcatagcgtc caacctcggc cgcctggtgg tgttcaccaa
     2581 cccgaagcgc gactttgtgg cgatcgagcc ggtcagccat gtcaacaatg ccgtcaacct
     2641 gctgcaggcc ggggcgggcc aggtcgatga gctgggcatc caggtgctgg caccgggcga
     2701 atccatgagt gcccagatga gcatccaagt ggagtccgcc gcatgagctg gacgaccacg
     2761 gttgcgcagc ccagcgaact gggcgaatcg ccgttctggc accccgacga gcagctgctt
     2821 tactgggtcg atattgcggg taaaaaaatt caccgcagca atgtgttcat gggcacggtg
     2881 gaaagctggg ccatggcggc gccgcatgag ctggagcccg gctgcatcgc accggcgcgc
     2941 agcggcgggc ttgtggttgc gctgcgcgac ggcatctatc gggcgcggac ctggggcggt
     3001 gcgctggaga agctggctga tgccgcgcac gacccgcgaa ccacccgctt caacgacggc
     3061 aaatgtgatc cgctgggtcg cttctgggcc ggcacgatct acgagccgcg cgctgcgcgc
     3121 gaagcggcgc tgtattgcct ggacggccgg gcgggccaca caccggcgct ggtgcgcatg
     3181 gccggcgacg cgaccgtggg caacggcctg gcctggtcgg ccgacagccg gaccctgtac
     3241 tgggccgaca cgaccggcca tgtcatccgc gcctgggact gggacgccgc cggcaacacc
     3301 ctggcccggc agcgcgtgtt ccggcagttc ccgcccaagc cgcccggctg gcaggccggc
     3361 atgccgggtt atggcggccg acccgacggc gcagcagtgg acagccaggg caattactgg
     3421 gtggccatgt acgagggcgg gcgcgtgctc aagctgtcgc cgggcggcga actgctggcc
     3481 gacatcccca cccccgtggt gtgcccgacc atggtgtgtt ttggcggcga cgacctgcag
     3541 acgctgtacc tgaccacggc gcgtcacggg cgacccgccg ccgaactcga agtactgccg
     3601 cagtccggtt gcgtgttttc catgcgtgtc gatgtgcccg gtctgcccgt ccattttttc
     3661 gcggacgcgg gttgaagccc ccaaaaaagc gaacagccgc cgttcaaaaa tatcaacccc
     3721 ggccgaccgg cgtcccctta ccatcagcac catggactca gcgcttcaat tgatcatcga
     3781 ccaggtgcag gcggcagccg ccagcggcac gccactgcgc atccgcggcg gcggcagcaa
     3841 ggacttctac ggccagacgc tgcagggcga ggtgctggat gtcacgccgc tggcgggcat
     3901 caccagttac gagcccaccg aactggtggt caccgcgcgg gccggcacgc cgctggccga
     3961 gctcgaggcg gtgctggcgg aacgcggcca gtgcctgcct ttcgagccgc cccacttcgg
     4021 gccgggcgcc acggtggggg gcatggttgc agccggcctg agcggcccgg cgcgcgccag
     4081 tgtgggtgcc gtgcgcgact acgtgctggg cgtcaccctg ctcaacggca agggcgagtt
     4141 gctgaccttc ggcggtcagg tcatgaaaaa cgtggcgggt tacgacgtct cccggctgat
     4201 ggcgggtgcc atgggcacac tggggctgct cacggaaatc agcctcaagg tgttgccggt
     4261 ggcgccagcc gaagccacgc tgaagttcgg aatgagccag tcagaggccc tggcaaagtt
     4321 gaacagctgg ggcgggcagc cgctgccgct caacgccagc tgctgggtcg acgacgccgg
     4381 tgcccccacc ctgtacctgc gtttgcgcgg cgccgtcgca gcggtcgagg ccgcctgcaa
     4441 aacactgggc ggcgagcgcc aggacaatgc cgcgacggca cccgactgga ccttgtgccg
     4501 cgaccagcag ttgccgtggt ttctggagcg cggcgagcag gacttgtggc ggctgtcggt
     4561 gccgcagacc gcaccggtgc tggacctgcc cgagccgccg ctggtcgaat ggcacggggc
     4621 gcagcgctgg gtgcgggctg ccgccggtga ggcgcagcgc ctgcgcgatg ctgctgcggc
     4681 tgttggcgga agcgctacgc ttttcatagc tgatggcgct catgtggaaa gggctgaggg
     4741 ccgatttgac cccttgaagc cacctctgga ccggattcac cgccagctca agaaagagtt
     4801 cgatccctcc gggattttca atcgcggccg cctctacccg gacttctgat gcaaacccac
     4861 ctcgcccccg aattcaagaa caccgccgac ggccaggcgg ccgaggcgat tttgcgcaaa
     4921 tgtgtgcact gcggtttctg cactgcgacc tgcccgacct accagctgct cggtgacgaa
     4981 ctcgacggcc cgcgcgggcg catctacctg atcaagcagg tgctcgaagg ccaggcgccg
     5041 acgcgcaaga cccagctgca tctggaccgc tgcctgacct gccgcaattg cgagaccacc
     5101 tgccccagcg gcgtgcagta cggccacctg atcgacatcg gccgcaagct ggtcgatgaa
     5161 cgggtgccgc ggcccacttc cgagagcgtg gtgcgctggg cgctgaagga aggcttgccg
     5221 tcaccgctgt ttgcaccggc catgaaactc ggtcaggccg tgcgtggcct gttgccggcg
     5281 gcgctgaaga acaaggtgcc cgcgcgccag ccggcgggcc agtggccgca gcgtgaacac
     5341 gcccgcaaga tgctgatgct ggccggttgc gtgcagcccg ccatgatgcc caacatcaac
     5401 accgcgacgg cgcgtgtgct cgacgcggcg ggcatccaga ccgtggtggc agccaaggcc
     5461 ggctgctgtg gcgcggtgaa gtttcacctg aacgaccagg acggcggcaa ggccgagatg
     5521 cgccgcaaca tcgacgcctg gtggccgcat gtggaggctg gtgtcgaggc catcgtcatg
     5581 aacgcctcgg gctgcggcgt caccgtcaag gagtacggtt accacttgca ggacgatgcg
     5641 gcgtatgcgg ccaaggcggc gcgtatcagc gaactgacgc gcgacctgag cgagctgctg
     5701 cccgaactgg tggcgtcgct gcgcggccgg gtggccgcca gcgctgcgcc tatcgcgttt
     5761 catccgcctt gcacgctgca gcacggccag cagctgcgcg gcggtgttga gcaacacctg
     5821 ggcgccctgg gtttcgacgt gaaggtcgcc agctgcgaag cgcatctgtg ctgcggttcg
     5881 gccggcacct actccgtgct gaacccggag atcgccttcc agctgcgcga ccgcaagctg
     5941 gcgaatctgg gcgagatggc gcccgaggtg attgtgtcgg ccaacatcgg ctgcatcacg
     6001 cacctgcaaa gcggcacggc cacgccggtg cggcactgga tcgaagtgct cgacgaggcg
     6061 ctatcaaata aatagctgct tgcgcccgtc catcaagggc tgaaggcaac tttcttctga
     6121 aatcaggcgg ccagcgcagc ttccacgtct ttcgccaggt cggccggttt gtcggtcggt
     6181 gcataacgcc ggatcacctg accgtccttg ccgaccagga acttggtgaa gttccacttg
     6241 atggccttgc tgcccagcag gccgggcgct tcggacgaca gccacttgta cagcgggtgc
     6301 gcggccgggc cgttgacgtc gatcttgccc atcattggaa aactcacgcc atagttgacc
     6361 tggcagaact cggcaatctc gccgtcggcg cccgggtcct gggagccgaa ctggttgcag
     6421 gggaaaccca gcaccaccag gcccttgccg gtgtaggcct tgtgcagctc ttcaaggccg
     6481 ccgaactgcg gcgtgaagcc gcattggctg gccgtgttca cgatcagcat gaccttgccc
     6541 ttgaattgcg aaagcgcaat gtcctggccg ttgatctgcc gggcttcgaa gtcgtaaatg
     6601 ctgctcatgg cgcgctcctg gttggacctg ccactgtact gcagcgaatg aacggagggg
     6661 ggatgttttt ccgccgggcc gccccgagga aaaatgcgcc ccctcggggg gcagagagct
     6721 acgcgcagcg agcgaacgtg ggggttattt ctgcggcgcg ggagccagcg ctgccagcgc
     6781 ggcggccagc acaatcaggc tgccgcccag cagcgtgcgc gggctgaatt ccgctgcgcc
     6841 cagtgcggcc gccgacaggc tggcaaacag aatttcggtc agcatcacga gtgcggtggt
     6901 gctggccgcc aggcgcgccg cgccatactg cagcgccatg ttgctgagca ggaaggccac
     6961 agccaggccc agcgcgacgc cggcccacga ggtgctccag accggcgccg gcacggtgtg
     7021 ccaggccatg cccagcaatg cggtggcagt ggccatgagg ccgccaccgc caaacatggc
     7081 catcatgcgt gattcactgg gcgtgtacgc cagcttgcgc agcagcacgt tggtggcggc
     7141 aaaactcagg ccacccatca gggccagcca gtcggcggca ctgtgcggca ccggccaggg
     7201 cgagtccggc gatttcagca cgatcagcac acctgccatc gccagcacca ggcgcagcag
     7261 cgaagcgcgg gtcggttttt ctcccagcag gccccaggcc agcagcacgg accacgccgg
     7321 catcaggtaa aacagcagca ccacccgaac cacgtccccg acggtcacgg cccagttgaa
     7381 gccgacgttg gtcagcccgg cggcgacggc cagccaccag agctgcggat gccgcgcgaa
     7441 gctgcgccag ccgccgggcc gccatgccga gatgcaggcc agcgaaaaga agtagatcag
     7501 cgcggtggac cacagggggt gcagcccctg cgactgcagc tcgcgaaacg gccaccagga
     7561 cacgccccag acaaaggcgt tgaagaccag tgccgatgcg gccagggcgg cgccacgggg
     7621 ggtcacggct ttcggctccg cgggaccacg gcctggccgc atgtccatgc cggccgcatc
     7681 agtgcaattt gtcgctgcgc gcgatggtca tcagatgatc gatctgctct ttgtatttga
     7741 tgcagttgct tttgtgccag cgctggatca gccacatgaa gccggccacc accacgccaa
     7801 aggcggtgat cgcgccgaag gccgacaggc ccatgccggt ggacaggctg tagccggcac
     7861 ccagcgccag gatgcaggcc tgctcgttga agttctgcac ggcgatggag cggccggcgc
     7921 ccatcaggtt gtggccgcgg tgctgcagca gcgcgttcat cggcaccacc aggaagccgc
     7981 ccaggccgcc cagcagaatc aggaagggcg cggccaccca gacattggtg atgaaattca
     8041 tcaggatcac cagcacgccc atggcgatgc ccagcggcat cacgctggtg gcgtgctcca
     8101 gccgcatgcg catggacgcc aggatggcgc cagcggcggt gccaatggcc accacgccca
     8161 ccagcgacga cgcctgcgtg gtgctgtagc ccagggccgc cgcgctccag gccagcacga
     8221 tgtaacgcag gttgcccgac acgccccaga acagcgtggt ggtcgccagc gaaatctggc
     8281 ccagcttgtc gcgccacagc gccgagttgc agttccagaa atcgggcagg agttcgagct
     8341 tgttgcgcgg catcggacgc atctccacgc cggtcagcgg aatgcgcgta ttgaaccagg
     8401 cggcaatcgc atacagggtg atcagcgcgc agatggcggc ttcgggcggc gtgtcgatgc
     8461 cggtgctgat gacggggaag tcgaacgaca gcagcaggtg cgacaccgaa tggccgacca
     8521 gctggccgcc gagcagcaca ccgaggatga tggaggcaat cgtcaggcct tcgatccagc
     8581 cgttggcctt gaccagctgg gaggccggca acaactcggt gaggatgccg tatttggccg
     8641 gggagtaggc ggcagcaccc aggccgacaa tcgcgtaggc catcaagggg tgtgtgccga
     8701 acagcatcat caggcagccc accaccttga tggcgttgct gatgaacatg accttgccct
     8761 tgggctgcgc atcggcaaaa gcgccgacaa agggcgccag caccacataa aacagcgcaa
     8821 acatcggcac cagcgcggcc ggctgccatt tgggcgcgcc actggttctg agcagctcca
     8881 cggcggcgac gaacagcgcg ttatcagcca gcgagctgaa aaactgggct gccatgatgg
     8941 tgtaaaaacc gcgcttcagc ttcattgaac ggtgttgatc actgtggcga atgcatcagg
     9001 cagtctgatg cggcacgata aaagtgaatg tctcctgact atcaaggttt atagcacgcg
     9061 gcaggatggc agaatcgaca aataggtctt gacaagcctg cgttaacccc cgccccgtcc
     9121 tgttccccca ctaccacgag ccgtttcctg acatgccccg tcccattctt gccaccgttc
     9181 atgccgccgc cctcagccac aacctggcgc gcgcccgcgc tgctgcgccg gacgcccggg
     9241 tctgggccgt cgtcaaggcc aatgcctacg gccacggcat cgagcgcgtg tttgatgcat
     9301 tgcgcagcgc cgacggtttc gccctgctgg acctgtccga ggcccagcgc ctgcgcgcgc
     9361 tggactggcg cggccccatc ctgctgctgg agggttgttt tgacgcccgc gacctcgagc
     9421 tgtgctcacg cctgggcctg tggcacgcca tccattgcga cgagcagatc gacatactgg
     9481 cggcccacaa gacccaggtg ccgcaccgtg tcttcctcaa gatgaactcg ggcatgaacc
     9541 gcctgggctt cacgcccgag cgttaccgcg cggcctggac gcggctcaac gccttgccgc
     9601 aggtggaaga aatttcgctg atgacccatt tcagcgacgc cgacggcgcg cgcgggataa
     9661 cccaccagct gcaggttttc gaggcggtca cgcacgacct gcccggcgag cgcacgctca
     9721 gcaacagcgc ggccactctg cgccatggcc tggccatcgg cgagaagtcc gactgggtgc
     9781 ggcccggcat cgcggtgtat ggcagttcgc ccgatttccc cgagcacagt gccgccgact
     9841 ggggcttgct gccgaccatg acgctggcgt cgaaaatcat tggtgtgcaa cagctggtgg
     9901 cgggcgacac ggtgggttac ggctccagct tcaccgccga cggccccctg cgcatcggcg
     9961 tcgtggcctg cggttatgcc gacggctacc cccggcactg cacgacgggc acaccggtgc
    10021 tggttcacgg cgtgcgcaca cgcatggtgg gccgcgtcag catggacatg atcacggtgg
    10081 acctgacacc ggtgcccgat gccggcatgg ggtcggacat caccttgtgg gggcgcgcgt
    10141 ccaacggcgc cttgttgtcg atcgacgaga tcgcagcggc tgcgggcacg gtcggttatg
    10201 agttgatgtg tgccgtggca gcacgcgtgc catttcaggt cgattgagcg gcagcgcgtt
    10261 gtccgctgag ctgatgctca acgcttgcgc aatcccagca ccatcaccgc gcccaccacc
    10321 agcagcgcgc cggccacctg caccggcgca atcgcctgac ccagaatcag ccaggccagc
    10381 accagcgcaa acaccggctc cacattcatg atggccgagt tgccgaccac gcccagtttg
    10441 ggcagcacgg tgaacatgat ggtgaaggcc gtgccgtaca gaaaggtcag cgcagccagg
    10501 ccccaccagc cgatggccgc ctgcggcaaa tggaagccgc cctgcacggc cacgcccgcc
    10561 agcgcgaaca ggccgacgat gctcatggtg gtcagggtgc gcacacggcc atcgacgtcg
    10621 ccggcctcgt gctgggtcag caccagcgcc acgccaaacg tggccgccgc cgccagcgca
    10681 aaggccacgc cggtgccgat gcggccccat tgcccggccg cgcccaggcc cgaagcggcg
    10741 cccagcacgt cgagcgccag cgccagcccg atcaggatca ccggcatggc cagcagcagg
    10801 gcgcgttcgg gcgagcggcg gtacagcacc cagtcccaca gcgccgtcca gatcgggtag
    10861 gtgttgaagg ccagcagcgc cagcgccacc ggcagccgcg ccacggccga atacaggcac
    10921 aggctttgca cggcgatcaa caggccgatg accggcagga tgcgtttgtt gcgtgcggtg
    10981 aagcgcagcg gcacacgctg ggtggccagc agggccgcca ccgccagggc agtgaccacg
    11041 ctgcggaagg ccaccgctgt ggccacgtcc acgccgttgt tgaaggccag gcgcgccgcc
    11101 acgtggttgg cgcccatcat cagcgcgatc agcagcaggg tggcaaaggc ggcgccgctc
    11161 agggcccggc gggtgctcat gggtacagcc catgaacacg ggcgtgcagg cgcgccaggc
    11221 cgagcagcgc gtcggtgaag ggggtgggta cctgcgtcag tttgcccagt tccttcacca
    11281 cggtgaccag ggcgtcgagc tcgaccgcct tgccggcttc cacgtcctgc agcatggagg
    11341 ttttgaaggc gccgagcttg agtgtcaccg cgtgccggtc ttccggctgc tgggcgatcg
    11401 ggatgccgat gcgtgcgcct atctctttgg cttcgagcat gatcttcgag atgaaatcgc
    11461 gcaccagctc gtcgccgagg atgcggtcgg tggtggcgcc ggtgaaggcg ctgatcggat
    11521 tgaccgtcat gttgccccag agtttgtacc aggcgtcttt ctggatctgc gccgacacgc
    11581 tggccgcaaa gccggcgcgc accagcagct ccgccagcgc ctgcacacgt gggctctgcg
    11641 cgcccgaagg ctcgccgatg atgaggccgt tgccgaaatg gtggcgaatc acgccgggtg
    11701 catcgaccga gcaactcgca tgcaccacac agccgatgat gttcgccgcc ggaatggccg
    11761 cggcaatctc gccggtggcg tccaccgctt tcagttgtgt gccggcgtag gggccgccaa
    11821 aaccctggaa gaaccaccag ggcacgccgt tcatcgctgt caggacgagg gtgtcggggc
    11881 cgatgagcgg tgcgatctgg cgtgccacgt cgagcagtgc gggtgctttc accgcgatga
    11941 ccaccaggtc ctgcacgccc agcgcggccg ggtcggcgct ggtttgcacg gccgcggagt
    12001 ggatgtctgt tccgcttgcc acacgcaggc cgtggcggtt cagcgcttcc agcgttgcgc
    12061 cacgcgccac cacgctaacg ctgcagcctg ccgccgcgag tccggcgcct atccagccac
    12121 cgatggcacc ggcgccgtag atacacgctt tgttgatggt tttatgagtc attttggctt
    12181 ccagcccttt gttgacgcgc gcaaggcgct ctagttttga tagctgttgt cgatccggtc
    12241 gacctgccgc accagcgcgt caaagacatc ctggccggcg ccgtaggccg gctggaactg
    12301 cagcgcgtcg cgcgtggatt tctgcgccac ggcttctgtc accgcaatcg ccgggcccat
    12361 gctgaaggtg gccagctgga tttcgcaggc acgctgcaac gtccacaaaa tggcaaaggt
    12421 ctgcggcagg gcgtggcccc aggtcagcag gccgtggttg cgcaagatca ctgcctgccg
    12481 cgtgccgatg ttgttgacca ggcgcgggcc ttcctcggcg tggatggtga tgccttcgaa
    12541 gtcgtggtag gccaccttgt catgcagctg ggccgtgtag aagttggtct gctgcaggcc
    12601 gccctgcagg caggcgaccg ccacgccggc ggtggtgtgg gtgtgcatca cgcagtgcgc
    12661 atcgggcagg ccgtcatgaa tcgccgcatg cacggtgaaa ccggccggat tgacccggtg
    12721 tgtcgagccg tccagcacct tgccctgcag gtcgatcttc accaggttgg aagcggtgac
    12781 ttcgctgtag tgcaggccga aggggttgat cagaaactgc ttgttcccac cggtgacgct
    12841 gtccggcagc cgcaccgtga tgtggttgta gatcatctcg gtccagccga gcatggcaaa
    12901 cacgcggtaa caggcggcca gctcgacgcg ggttttccat tcgtcgggat gtatcccggg
    12961 tttttgcagg acggcgttca tggggagtct ccagtcaata ggttttgctg acgggcgcgg
    13021 gcgcgacggc tgccctgaaa gcggcggcca gggcgctggt ggcccggacc agcagatggg
    13081 tgtccagccc cacggccaca aaggtggcac ccagagccag gtacttgcgc gccgtggctt
    13141 catcgggcgt caggatgccg gcggctttgc ccgccttgtt gatgcgcgtg atggcgtcgg
    13201 caatcgctgc ctgcacctcg ggatgggccg ggttgcccac atggcccatg gacgcagaca
    13261 ggtcggccgg gccgatgaag acaccatcca caccgtccac ggcggcaatg gcgtccaggt
    13321 tgtccagcgc cagctgtgtt tctgcctgca ccagcaggca gacctgtgca ttggcttcat
    13381 gcgcgtactg cgggtaacgg ccccagcgcg aagcgcgcgc gccgcccatg ccgcgtatgc
    13441 cttgcggcgg gtaacggcta gcccggacca gcgcagcggc ttgcgcgggt gtgtccacca
    13501 tgggcaccag caaggtctgc acgccgatgt ccaggtactg cttgatcagc gcttcgccga
    13561 catggccgtg gcccatgggc acccgtgcga tcgcgtgcga gcccgggtag ccggccatca
    13621 cctgcagctg ggccaggatg ctgttgatgt cgttgggggc atgttcgccg tcgatcagca
    13681 gccagtcaaa accggcaccg gcgcagatct cggtgctgta ggggctggcc aggcccagcc
    13741 agaggccgat ctgcgcgcgc ttgtcggcca gggcctgctt gaagggattc aggggtgttt
    13801 gcatggggtg tctttaaaca aagtgaaatt caaaacgacc gaggggtccg tagtcggcgt
    13861 cgaaacggtc accggctttc gcggcgaccg gccgggtgaa ggagcctgcc agcacgacat
    13921 cgccggcttg caggcattcg ccccaggggg cgagtttcat ggccagccag gcgacaccaa
    13981 tggccgggtg gccctgcacg ccggcggcca ggccggtttc ctcgaccacg ccattctggc
    14041 gcaggatggt gccgacccag ggcaggtcga cgtcgtgcgg cgacaccttg cggccgccca
    14101 gcacgatgcc ggcattggcg gcgttgtcgc tgatggtgtc ctgaaccttg cgcatggctt
    14161 tgctgtggcg gtcgaactgc tcgatgcgtg cgtcgatgat ctcgatcgcg ggctgcacat
    14221 aatcggtggc gtccagcaca tcgtccacgg tgatgtgtgt gccttccagt tttttcttca
    14281 gcacaaaggc cagctccact tccacgcgcg gcgcgatgaa gcgggctgcc ggaatgtcgc
    14341 cgggttcgaa aaacatgtcg tccagcagcg tgccgtagtc gggcgagtcg atctggctgg
    14401 agatctgcat cgcgcgcgag gtcaggccga tcttgtggcc cctcgccaca cgccccccgg
    14461 tgattttcat ttgcacccag gcgcgcgaaa tcgcatagcc gtcctcgacg gtcatgccgg
    14521 ggtaacgttt tgaaaaatgc tcgacctgca cgcgggtctt ctcgctgtgg tccagttcgg
    14581 cggccagctg ggcaatcagt tccgggctga acatggtcag gttttctgga agaggggatg
    14641 gaggttgctg tgtttgccgt cgtagacctg gccggggctt tcgtccagct gcagcgtgat
    14701 gccgatcagc cggtccttca tcaaggggtc aaaatgggcc gtagcgagcg ccagcaaagc
    14761 gtctccagct ctctttttgg tagcgttcga gcggccggca gccatgcgca gattcaggta
    14821 aacaaagccg tagtcccgct tcccgtccgc caccgcgtaa tgcgcggccg ggtaggccag
    14881 cacccgcgtg ccgccggtgg ggaagacctg tttgccctcc tcgtctttca cggccagcat
    14941 ggtgcccgcc agcgcgcggc acagtgccgt catgtcggtg gcggtctcga ggttgggggt
    15001 gtagaggatg acgaggtgtg gcattgtctt gtttggtcag agccggctca cggcctggta
    15061 gccgtgcgcg gacgaggcct gcgcggcggg aatggcggcg ccggtttgcg gcgtgaccgg
    15121 gaacaccgcg ttgatctgcc cggtgcccga agcgccgaag tagggcgtga tgacctcggc
    15181 ctgagcgtcg tagtctgacc agcccaaggc gcccagcagc atggcggtgt catgcatgaa
    15241 accttcgccg tggcctttgg ccgcgtattc gggcagcatc gcgcagaacg ctttccagtc
    15301 gccgcgctgc cacatgcgca ccacctcatg gtccagcgtt tcgaggaagg ggctccagac
    15361 cttgaatgcg tagtcgggcg ccaggccgtt ctgcgcaaaa cggtgcgaca gcgagccgct
    15421 cgcaaaaaag gccacggtgc cgtcgtactc atcttcaatc gcgcggcgca tggcccagcc
    15481 caggcgcgcg ctgtcgttga ggtaatgcgc ctggcacagc gccgagaccg acaccacctt
    15541 gtagtgctgg tcggcattca tgtaacgcat gggcacgagc gtgccgtact ccgggttcag
    15601 cgtggtggcg tcgtgcgcca gcgtttccac gccatgcgca ttgcagaccc tggccagcag
    15661 ctgacccagc gccgggttgc ccgggaactc gaaacccatg ttgctgatga agtgcggcag
    15721 ctcgttgctg gtgtacaggc ccttgaagtg cggcgcgcag ttgatgtggt aattcgcgtt
    15781 gaccagccag tgcgtgtcga acaccaccag ggtgtccacg ccgagagcgc ggcaacgccg
    15841 gtcgatttcc aggtggccgt caatcgcgtc ctgccgcgtg cctttgcgcg ggccgtccag
    15901 ctcgctgagg tacatcgaag gcacgtgcgt gattttggcg gcgagggcca gtgttcccat
    15961 gctcagactc cccaatgcgg aatgtgatgg ctaccgagcg aaaccgccac gttcttcggc
    16021 tcgcagaaca cttcgtaact ccaggtgccg ccttcgcggc cggtgcccga ggccttggtg
    16081 ccgccgaagg gctggcgcag gtcgcgcacg ttctggctgt tcacgaaaca catgccagcc
    16141 tcgaccgctg cggccacccg gtgggcgcgg ccgaggttct cggtccagac ataactcgac
    16201 aggccgtact gggtgtcgtt ggccaggcgg attgcgtcgg cttcatcctt gaacgggatc
    16261 aggcacgcca ccgggccgaa gatttcttcc tgcgcgatgc gcatgcggtt gtcgacgtcg
    16321 gcaaacaccg tgggcagcac gtagttgcca tttttcaccc gctccggcag gtccggtgtg
    16381 cccagcccgc cgcacagcaa ggtggcgcct tctttcgggc cgagttcgat gtagctgcgc
    16441 accttggcca gatggccctg cgaaatcatg gggccgatgg tggtggcttc gtcgagcggg
    16501 tcgccgacct tgatgcgttt ggcgcgttcc acgaatttgg ctgcgaagtc ggcgtaaatc
    16561 gattgctgca ccaggatgcg gctgccggcg gtgcagcgct cgccgttgtt ggagaaaatc
    16621 atgaagatcg cggcgtcgag tgcccgctcg aggttcgcgt cctcaaaaat cacaaacggt
    16681 gatttgccgc ccagctccat gctgaatttt ttcaggccgg cggcctgcac gatgcggttg
    16741 ccggtggcag tcgatccggt gaacgagatg gcacgcacgt cagggtggcg gcacagcggc
    16801 tcgccggcgt ccttgccgta gccgtgcacg atgttgagca cgccgggcgg aatgccggct
    16861 tccagcgcca gctcccccag gcgcgcggct gtcatcggcg agagttcgct catcttcagc
    16921 accgcggtgt tgccaaaagc caggcaaggc gcaaccttcc aggtggccgt catgaagggc
    16981 acgttccacg gcgagatcag tgcgcagacg ccggtcgggt ggtacagcgt gtagttcagg
    17041 tgcgtctcgg tcgggtaggt gtggccatcg acccgcgtgc acatctcggc aaagtaatag
    17101 aagttgtcgg ccgcgcgcgg gatcagctgc ttgccggtct gcgcaatcac ctggccacag
    17161 tcgttggtct ccatctgcgc gatctcgggc acatgtgcgg cgatcaggtc gccgagcttg
    17221 cggaccagtt tggcgcgctg cggtgccggc atgccggccc acttcggaaa ggcctctttc
    17281 gcagcggcca cggcggcgtt gacttcggct tcgccgccag cagccacttc ggccagcacg
    17341 tcctgagtgg cggggttgac ggtctcgaag tagctgctgc cggcgacggc ccggccattg
    17401 atgaggtgat ctatttttgt catggtcatt caatcgtatt gataagcgag cccaggccct
    17461 cgatgctggt aacgaccacg tcgccgggcc ggcagtccac cacaccgtcg ggtgtaccgg
    17521 tcaggatcag gtcgccgggt tgcagtgtca tgaagctgct gaaatattcg atcaggaagg
    17581 gcacgtcgaa aatcatgtca cgcgtgttgc ccgtttgcgt gatttttccg ttgacggtgg
    17641 tctgcagcgc cagcgccatg gggtccggca catcggccgc gtccaccagc caggggccca
    17701 gtggcgtgca ggtgtcgcgg tttttcacgc gcagattggg ccggtaccag ttttcgagat
    17761 agtcgcggat cgcatagtcg ttggcgatgg tgtagcccgc gatgaagtcg tacgcgtcgt
    17821 cacggctgac ctttttggaa gtcttgccga tgaccaccgt gagttcacac tcgtaatgca
    17881 tgaactgcac atcggccggc cggcgtgtca gttggcggtg gccggtcagc gtgccttcgc
    17941 ccttgatgaa caccaggggc tcttcgggcg ccttgaattc cagctctttc gcgtggtcag
    18001 cgtagttcag gcccagcgca agaatggtgc gcgggcgcgg cgtgggtgcc aggggcggca
    18061 gccaggtcac cgcgtcttcg gcgacgagtt gtccgttggc gagcaggagc tggccgccgc
    18121 gttctgtggc cgaatgcacc tggccttgat gaaccacacg tgcgtgtttc atgtggcctc
    18181 ccgaaccagc gtgttgctca gcgtgccgaa gccgggtgcc gtgatgtcaa tctggtcacc
    18241 ggctctggcc agtggtctgc ccaggtcgca accgagcatc agcacatcac cagcgcgcag
    18301 ggtcatgaac tcaccgacat cggccagcag ttggtcggca gggcgcacca gctgcgaaaa
    18361 actgacggcc tgtttcagct cgccattgat gcgcacttcc agtgtgaagc ttgaaggatc
    18421 gccagcttcg ctggcaggca ccagtcgctg gccgatgccg agaaaaccgt ccaggcattt
    18481 gaacttgacg ggcggccgga aaaaagacgc atggggaatc gacaggtcgt tcatcagcac
    18541 atagcccgcc acgacgccgg cctcacccat caccatggcg atgctggcgc cgacttcgac
    18601 ctcggggacc cgagccggca cggggatgct ggcgccgctg gcgctccagg tgttggccgg
    18661 cttgacatac agcaccggcg cctggggtgg cgccttgtag ggcggctggt tcatttgcgg
    18721 cgccagcgcc tcgacttcgg cgcggaagtt cagcagcacg ccgtagaccg tgcccgacgg
    18781 catgaaactc atggctgctc cagttcaatc accttgtcga gcagtgcgta aagctgcgac
    18841 aaattttctt tacccaggga ctgctccatc cacgcgtaat gcgtttctat ggtttgcgac
    18901 agggtgttga ccagttgtgc gcccttgtcg gtggcctgaa ccaccgtgcg ccgctggtcc
    18961 accgaatcgc gcgagcgctg gatcaggccg tcgcgctcca tgcgggtcag cacgcccgtc
    19021 aggctggggc cgaggatgaa ggcttcgcgc gcgaccttgc cggtttcgac cacgccgtgt
    19081 tcacccagca cccgcagcac ccgccactgc tggtcgctca gtgcatgttc gcgcaggcgc
    19141 ggccgggtgt gtgccatcac ggattcgcgt gcctgcagca gcaggcgggg caggttgcgg
    19201 tgaataaaag gcgtgttcat gggcaaggag gatttattta atatattaaa tgatttgaag
    19261 cttgctgcca gccgcgcgag gccgggacta acccgcactg tgaataaata cagtaatatc
    19321 gccggatggc caaagacaag acgatttaca cctgcaccga gtgcggcggc accagcccca
    19381 aatggctggg caaatgcccg cattgcgggg cctggaacac gctgatcgag tcgattgccg
    19441 aaagccagtc gccggtgaaa aaccgttttg cctcgctggc caaaccgtcg gaagtggcga
    19501 ccctggccga catcgaggcc accgacatgg cgcgtacgcc caccgggcat gaggagctgg
    19561 accgtgttct gggcggcggc attgtcgaag gcggcgtggt gctgataggc ggcgatcccg
    19621 gcatcggcaa gtccacgctg ctgctgcagg ccctcgattc cctgcagcgc gccggcctcg
    19681 acactctgta tgtgaccggc gaggaaagcg gcgctcaggt ggcgctgcgc tcgcgccggc
    19741 tcgggctgga cggctctcag gtcaaggtgc tggccgagat ccagctcgaa aaaatcctcg
    19801 ccacgctg
//
LOCUS       DAIDFG010000014        19805 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_14_length_19805_cov_18.083443, whole genome shotgun sequence.
ACCESSION   DAIDFG010000014 DAIDFG010000000
VERSION     DAIDFG010000014.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 19805)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19805)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19805
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14_length_19805_cov_18.083443"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3551 a         6134 c         6419 g         3701 t
ORIGIN      
        1 agagcagctc acgttcaagc gtgaaatgga aaaactggaa aaagacatcg gcggcatcca
       61 ggacatgaat gccctccccg acgcgatctt cgtgatcgac gtgggtttcc acaagattgc
      121 catcctcgaa gccaagaagc tgggcattcc gctgatcggc gtggttgact ccaaccactc
      181 gcccatcggc atcgattacg tgatccccgg caacgacgac tcgtccaagg ccgtggcgct
      241 gtatgcccgc ggcatcgccg acgcgatcat cgaaggccgc gctgccgccg gcaatgaagt
      301 cgtcaagatg gtctcggccg aaagcagcga cgaattcgtc gaagtggaaa acgccgcagc
      361 ctgatcgtcg cggccctgcg cctgacgcga aaaaggggct cgcgtagccc ctttttcaca
      421 gccttgtcca aaggcacccc catttaattt gaagtcaaca cgacacacgg agaactgaag
      481 atggcaatca cagcaagcat ggtcgcagag ttgcgcgcaa aaaccgacgc accgatgatg
      541 gagtgcaaaa aggcactgac cgaggccgat ggcaacttcg aaaaagccga agaaatcctg
      601 cgcgtcaagc tcggcaacaa ggccggcaag gccgcttccc gcattaccgc tgaaggcgtg
      661 attgccttcc acagcgaagg tgccgtgggc gcgctggtcg agatcaactg cgaaaccgac
      721 ttcgtcacca agaacgacag cttcctggct ttcaccgacg cggtggtcaa gggcatcgtg
      781 aagaacaacc cggccgacgt ggccgcgatc ggcgccatgc ctttgagcct ggacggtttc
      841 ggcccgacgg tggaagacgt gcgcaagggc ctgatcggca agatcggcga aaacatgagc
      901 gtgcgccgct tcaagcgttt tgaaggcggc aagctggcct cctacctgca cggcacgcgt
      961 atcggcgtgg tcgtcgagta cgaaggcgac gaagtgcccg ccaaggacgt cgccatgcac
     1021 gttgccgcga tgaagccggt gtcgctgtcg agcgaccagg tgcctgccga gctcgtcgcg
     1081 cgcgagcgtt cggtcgctgc ggccaaggct gccgaggatg ccgctgtggc caccgctgcc
     1141 ggcaagcctg tgcaatcccc tgaaattgtt gccaaacgta ttgacggcgg cgtgcagaaa
     1201 tacctcaagg aagtgtccct gttcaaccag cctttcgtga aaaacgacaa gcagacggtc
     1261 gagcagatgc tcaaggaaaa aggcacgacc gtgaaggcgt tcacgctgta tgttgtcggt
     1321 gaaggcatcg agaagaaggt cgacgatttt gcggctgaag tggccgccca ggtggctgct
     1381 gccaaagcgg cctgattgca cccgctgacc cgaaaggccg ctaaacttac gcccataaaa
     1441 acgacaggaa ctcccctcat gccagcctac aagcgaatct tgttgaaact ttcaggtgaa
     1501 gccttgatgg gagacgatgc cttcggcatc aaccgtgcga cgattgtgcg catggtcgag
     1561 gagattgccg aagtcacccg catgggcgtg gaagtggctg tcgtgatcgg cggcggcaat
     1621 attttccgcg gcgttgccgg cggctcggtc ggcatggacc gtgccaccgc cgactacatg
     1681 ggcatgctgg ccaccgtgat gaacgcgctg gcactcggcg acaccatgga caaggcaggc
     1741 ctgacgccgc gtgtcatgtc ggccatcggt atcgagcgcg ttgtcgagcc ctatgtgcgt
     1801 cccaaggccc tgcaatacct cgaagagggc aaggtcgtca tcttcgcggc gggcaccggc
     1861 aatcctttct tcacgaccga caccgcggcc gccctgcgcg gcgcggaaat cggggccgag
     1921 attgtgctca aggccaccaa ggtggacggc gtgtacagcg cggacccgaa gaaagacccg
     1981 acggccacgc gttacaccaa catcaccttc gacgaggcca tgggcaaaaa cctcgaagtc
     2041 atggacgcca ccgcctttgc gctgtgccgc gaccagaaac tgccgatcaa gattttcagc
     2101 atcttcaagc acggcgcgct caagcgcgtg gtgcagggcg aagacgaagg aacgctggtc
     2161 cacgtctgac gcctgccggt gtgcatgcca tccctttgca gaacaagaat cctccggaga
     2221 tttcaatcat gagcattccc gatatcaaga gccaggccga gcagaaaatg cagcagtcgc
     2281 tggagtcctt caagcatgcc ttgaccaaga tccgtaccgg ccgtgccaac cccggactgc
     2341 tggacacggt gcatgtggac tactacggcg ccatggtgcc gatcagccag gtcgccaatg
     2401 tggcgctgct cgatgcgcgc accatcagtg ttgcgccctg ggaaaaaggc atgggcgcca
     2461 agattgaaaa agccatccgt gactccgacc tcggactcaa ccccgcttcc cagggcgacc
     2521 tgattcgcgt gcccatgccg gccatgaccg aagagcggcg caaggagctg accaaggtgg
     2581 tccgcgccga aggtgaacac gccaaggtcg ccatccgcaa tctgcgccgt gatgcgaacg
     2641 acggtgtcaa gaaactcgtc aaggacaagc tggcgtcgga agacgacgag cgccgcgccc
     2701 acgacgaagt gcagaagttc accgaccgtt tcattgccga agtcgacaag ctggtcgcag
     2761 gcaaggaaca agacatcatg gcggtctaga ttgcccccac gttcgctcgc tttgcgtagc
     2821 tctctgcccc ccgagggggc cgctgcgcct gcggcccggc gaagccggtt ccgcggcccc
     2881 tgctggtttg agaccgggac ggtggctctt gttggcactc tttccctcgt ctgaccttca
     2941 tcttggccac caaacgcatc agtgacgaac ccgcctatgt cctgcaccgc tacgactgga
     3001 gcgagtccag cctgattctg gaagtgttca cacgtagcta cggccgcatt gcgctggtgg
     3061 cgcggggtgc gaagaaaccc agctcgaact tccggcccat tttgctgccg ctgcaacccc
     3121 tgcacattgc ctttggcggc gacgccgaga tccgcatgtt gcgcagcgcc gagtggcagg
     3181 gcgggcatgt catgccgacc ggcgacgcgc tgctgtcggg gtattacctc aacgagttgc
     3241 tgatgcgcct gctggcgcgc gacgacccgc acccggtgct gttcgacgct tatgccgcca
     3301 ccgtgcagtt gctggccagc cagaccatcg acacgctgga ggtggccttg cgcgcgttcg
     3361 agttgcgcct gctgcagggc atcggcctgc tgccggcgct ggacgcccag accgccaccc
     3421 tggcgccgct ggagccgcag acgccttatg tgctggtggc cgaggccggc ctgcgcgagg
     3481 cgcatgacga cgaccgcgcc agcctgcagg gccggcagtg gctggatttg caggacacgc
     3541 tcagggacga tgcaccgttt accgacacgg tgcaggcctg tgtgaaccat ttgccggcgc
     3601 tcaaaaccca gctgcgctcc ctgctgcact accattgcga tgtgaaggtt ttcaaaaccc
     3661 ggcagatgat gatcgatctg caggctttct aagggatcaa tgcccaccat ggctcctgta
     3721 cacgcccccg ctccccgcca ccgcacagcc ctgtcggtca acctcaacaa ggtggctttg
     3781 ctgcgcaaca cccggcacct gggcatcccc gatgtgctgc gcgccgccga gctgtgcctg
     3841 gaggcaggtg cccagggcat caccgtgcat ccgcgtcccg acgagcggca tatccgcagc
     3901 gatgacgtct acgagctggc cgcactcatg aaggactggc ccgaccgcga gttcaatgtc
     3961 gagggcaatc cgctgcagaa cctgatggag tttgtgcgca acctgtcggc gcgcggcctg
     4021 ccgctgcacc agtgcacctt tgtgcccgac agcgaagggc agttcacgag cgaccatggc
     4081 tggagctttc cccaggatgc ggcccgtttg aagccgctga ttgaagaagc ccacgaccgg
     4141 ggcgtgcgcg tcagcctgtt catggacccg ctgcccgaag ccatggcggc cgcccaggcc
     4201 gttggcgctg accgggtgga gctgtacacc gagacctatg cgagggcctg gggcacacct
     4261 gaacaagaca aggctttgga gccgtttgtc cttgcggggc gggcggcggc agctatcaat
     4321 ttgggagtga acgccggcca cgacctgaac cgcgacaacc tcaccgattt tctgcacgcg
     4381 gtgcccggcg tgctcgaggt gtcgatcggc catgcgctga ttgccgacgc gctggagctc
     4441 ggttacagcg ccaccgtcgc cgcttatctg cgctgcatcg acaacgcgtt ccccgtcgcc
     4501 tgatgggcac gttgttgcat cttcttctct ttgccagccg gggtcgcggg tctggctttg
     4561 ccagcccgca ggcgcagcgg ccccctcggg gggcagggag cgacacgcag tgcgcgaccg
     4621 tgggggccat atccggatga tttacggcat aggcaccgac atctgcgacg tgcgccgcgt
     4681 gcgcgccagc ttcgagcgcc atggcgagcg ttttgcgcag aagatcctca gcgacgctga
     4741 gttcgccacc tggcaggccc gcagtgcccg ctggcccgag cgcggcctgc gttacctggc
     4801 cacccgcttt tccgccaagg aggctttcag caaggccatc ggcctgggca tgcgtttgcc
     4861 catgacctgg cgccactgcg aaatcgccaa ggccgctagc ggcaagcctg aaattgtttt
     4921 acacggtgcg ctcaaggaat ggttcgaggc gcgcggcctc accgcccatg tcagcgtgac
     4981 cgacgaaacc gactatgccg ccagttttgt ggttgtagag aaaaacccgt tataacccaa
     5041 gctgtatgta cgcgaacagc tattaaactc atagtaaatc agagatccct tcatgagcca
     5101 acacgccccc ctgatcatcg atattgccgg cctgacctta agcaaaaccg acaaacgccg
     5161 cctcaaacat ccgctgacgg gcggcatcat cctgtttgcc cgcaactggg aaaaccgcgc
     5221 gcaactgacc acgctgtgca ccgagatcaa gaaaatccgc aaagacctgc tgatctgcgt
     5281 ggaccacgaa ggcggccgcg tgcagcgctt caagaccgac ggcttcaccc acctgccgcc
     5341 catgcgcgcg ctcggtgagt tgtggctgaa agacgcgatg gccgcgacca atgccgccac
     5401 cgcctgcggc tacgtgctgg gcgccgagct gcgcgcctgc ggcgtggact tcagcttcac
     5461 cccggtgctg gacctcgact acggtgaaag cagtgtcatc ggcgaccgcg cttttgcgcg
     5521 cgacccgcgt gtcgtcggcc tgctggccaa aagcctgatg cacggcctgc tgcaaagcgg
     5581 catggccaac tgcggcaaac actttcccgg ccatggcttt gtcaaagccg attcgcacac
     5641 cgacattccg gtcgacaggc gcagcctgaa agccatcctg gcggacgacg ccgcccctta
     5701 tgagtggctc aacaccacgc tgaccagcgt gatgccggcc catgtgattt accccaaggt
     5761 cgatgcgcgt ccggcgggtt tttcggagaa gtggctgacc tacatcctgc gcggccagct
     5821 cggttttggc ggcgccattt tcagcgacga cctcagcatg gccggtgccc gcctgctcga
     5881 cggcaagcag gtcagctaca ccgaagcggc gatggccgca ctcaacgccg gctgcgacat
     5941 ggtgctgctg tgcaaccagt cgataggtga aggccaggag gtggacgagt tgctggacgg
     6001 cttgacgcag ggccagctca ggcaacagtg ggaaccgctg gaagccagcg aagggcgccg
     6061 tctggacctg ctgcccacca cacctgcgat cgactgggac gcgctgatgc tgcacccggc
     6121 ctacatgcac gcgctcgggc tggttccctg agtttctttg agccttttcg tgccccagcc
     6181 tatatggcgt gtgcgcagac agctatcaaa taaatagcga acagcgcctg ctcagcggct
     6241 caccggccac ggcgcttgac cgccggcggc catgtgcgtc aggtacaggc gcaggtccag
     6301 ctcgaactga tggtagtccg gctccatgta ggtgcagagc tggtaaaacg ccttgtcgtg
     6361 cgccttttcc ttcaggtgcg cgagttcgtg caccgtgatc atgcgcagga actcgatggg
     6421 cacctccttg aacaggctgg ccacgcgaat ctcgcgtttg gacttgagct tgctgccctg
     6481 cacccgcgac accgtggtgt gggtgcccag cgcatgggca atcacctgca gcttgctgtc
     6541 aaacgccacc ttgctcagcg cgtcggcatt gcgcagaaag ctgttcttga tgtccatcac
     6601 gtagtcgtac agcgccttgt cggtgcgtat ctcgtgcgcg ctgcggtatt ttttgagcag
     6661 catggcgccc agccggtctt gtgcgacgag atcacgcacc tggctctgca gggtgtcggg
     6721 gtaacccgtc aggtagttca tggggttggg gagggaggcg ggcgaagggc ggaggcaatg
     6781 cgcgatttta gtcccgcgtc cgattcttgc taagcttgtg gcatgaacct gcccgccgcc
     6841 ccggaccctg ctgacgtgcc cgacaccgtg acgccgcgca tcaacctggc gctgcagggc
     6901 ggcggctcgc acggcgcctt cacctggggc gtgctcgacg ccctgctgga cgacggccgc
     6961 atagccttcg aaggcatcag cggtaccagc gccggcgcca tgaacgcggt ggcgctggcc
     7021 catggctttg cccgctccga aggcaaaccc cgggctgcgc ggcacgacgc ggcacgcgaa
     7081 gcgctggaca gtttctggaa cggcattgtc gacatgggcg cgctgtcgtc ctcgctctcg
     7141 caatcggtca gcaagattca gcaggcgccg ttcggcatcc tgttcggcct gatgggcggc
     7201 ggcaactggt ccagcaagct gtggaccgac gccatgacac gctactggtc gaatgcgctc
     7261 tcgccctacc agcgcaaccc cttcgacatc aacccgctga aagacttcct ggaaaagcag
     7321 gtggactttg agcgcctcgc ggcggcggcc gaccccgaca cgccccaggt gtttgtggtg
     7381 gccacgcgcg tgagcagcgg caaggccgag gtgttttgcg gcaagcggct gaccgccagt
     7441 gccgtcatgg cctcggcctg cctgcccatg gtgtttcagg cggtggagat tgacggcgac
     7501 cacttctggg acggcggtta ttcgggcaac ccggcgattc acccgctgat ctaccgctgc
     7561 gagagccgcg acatcgtgct ggtgcagatc aacccgatcc agcgcaacac cctgccgacc
     7621 aaacccggcg agatcatgga ccgcatgacc gagatcacct tcaacgccgc gctgattgcc
     7681 gaaatgcgag cggtcgattt cgtcaagcgc ctgctcgccg aaggcaagct ggaccccgcg
     7741 cattacaagg acgtgctgat gcaccgcatc gacggcggcc aggcgctgga aaaattcact
     7801 gccgccagca agtcatcgac ggacaagagc ctgattcatt cgctgcgcga cctgggcatc
     7861 gtctgcggca aagagtggct ggccaaacgc ttcgacgcac tcggcgtgaa aagcacagtc
     7921 aacatcgcgc gcgattacct ggatgacctg cgcatgccgg tggaaaaggc ggagccggca
     7981 cagatcgaac cgccgccacc tgagccgccg ccggtttttc tgccgggggc cgggttgctg
     8041 tagagcttgg gtggacgtgg ttactatcat tttgatagct ggtggcgccc gtcccgcttg
     8101 ggttaactgc cggtttgatg ctgtatagtg agataagcct atgctttcaa acactttttt
     8161 gaaccagagt cgccggtgtt ctctgactgg tggccatgtt attccacagt tttgcgggat
     8221 atatcaaagt taggcggcaa gaacagatgt ccatgcgaaa gtcggatctg ctgacacaac
     8281 gtatccgccg ctactccagc gcggagagaa tgacgttgct ccgaaatctc ggttatggcg
     8341 gggcggcagc ttgtctcgcc atcttggcag gtctcgccca agtgggcgcc aaggatccag
     8401 ctctgaaggt cgccgtctac gctgcttcaa tcgcactacc agcttggctt ctgatcggct
     8461 cggtctttga gtactacatt ttcctgggga agcaaagcta ccgtcacctt cgcagcaagt
     8521 ttgttatagc cttaacttcg acgctttatg tcgttgctgg cgtgggaatg ttcgctgcca
     8581 cgggcggtat cgcttggtat ctcgctcccg aggctgcgta tgccttcgca ttctccgcat
     8641 tttgcgcagt ggtcctaggc cttgcgtttc atgctcatct agcaggatgg tgggaccggg
     8701 aagtcgtttc gaagggaaag gacgatgtgg atggctaacg ccggactatc agcgagtaga
     8761 gcgttgccgt ctaacaggtc gttcgacgca gacacgcaac agcattgcgc agcaatgcgt
     8821 gctgttgagc gtacgcctcg cggcgcaatg ccgctgcgcg ccggtcaact ccgacgttag
     8881 atactgaagc atgtatagca cccgccgatc caaggcggaa tgtcagacca aacctcaagc
     8941 ctcgtgtcgc agcgccggga acaaaatgac gtcgcggatg ctggcgctgt cggtcagcag
     9001 catcatcagg cggtcgatgc cgatgccgca gccgccggtg ggtggcatgc cgtactccag
     9061 cgcgcgcaca tagtcggcgt cgtaatgcat ggcttcctcg tcgccgccgt ccttggcgtc
     9121 tgactgggcg agaaaacgcg cggcctggtc ttcagcgtcg ttgagctcgc tgaagccgtt
     9181 gccgaactcg cggccggtga tgtagagctc aaagcgctcg gtcacctcgg gccgtgcatc
     9241 gttggcgcgg gccagtggcg agatctcggt cgggtgctcc atgatgaagg tgggctgcca
     9301 gagtttttcc tcgaccagct cttcaaaata agtcacctgc aggctgggca gcgagcgggc
     9361 atcgagcctg tccttggcgc tgaatttgcc gagttttttc agggcggcca gcagccaggc
     9421 ggcgtcgtcc acatgggcgc cggcgtcggt gtgtttgagg atggcttcgc ggatggtcag
     9481 gcgttcaaac ggctggccca ggtccaccgg ccggccctgg tagctcaatt gcgtgccgcc
     9541 agtcacccgg gtggcgacgt ggcggatcag gtcttcggtg aagctcatca ggtcttcgta
     9601 gttccaccag gcggcgtaga actccatcat cgtgaactcg gggttgtgcc gcacgctgat
     9661 gccttcgttg cgaaagctgc ggttgatctc gaacacgcgc tcaaagccgc cgacgatcag
     9721 gcgtttgagg taaagctcgg gcgcaatgcg caaaaacatc tgctggtcca gtgcgttgtg
     9781 gtgcgtcaca aacggcttgg cattcgcgcc acccggaatc gggtgcagca tgggtgtttc
     9841 gacttcgaga aaatcgttgt ccaccatgaa gttgcgcatg gccgagacgg ccttgctgcg
     9901 ggcggcaaaa cgtttgcgcg cggcttcgtc ggtgatcagg tccacatatc gctggcggta
     9961 tttgacctcc tggtcggcca cgccgtggaa cttgtcgggc agcggccgca ggcttttggt
    10021 gatcaggcgg atgctggtgg cgtggatcga cagctcgccg gtcttggtct tgaacagcgt
    10081 accggtcgcg gccacgatgt cgcccaggtc ccagtgcttg aaggcgttat gaacctcttc
    10141 acccacgcct tcgttgttga tgtacacctg gatgcgcccg ccggaagggc cgaacgacgc
    10201 gtcctgcagc gtggcaaaac tggccttgcc catcacgcgc ttgagcatca tgcggccggc
    10261 cacgctgacg ttgacggcca gcggcaccag ctcttccttg gtcttgtcgt cgtactgcgc
    10321 aaacagggct tcggcccggt gttccggctt gacgtcgttc gggaaggcca cgcccttgcc
    10381 gtcggcctgg tgctgacgca tgaccttgag cttttcgcgg cgctcggcca tgagctggtt
    10441 ctcgtcatgc gcgggcgcgg cggcggggct ggtgatggtg ttggacatga aaaaggttct
    10501 cgaagaaaga gggcaggccg ggcgcacagg gcggcaggcc ggaaaaaccg gcgggaccgg
    10561 gcaaagtgcc aaacccctga ttttaggctg cgttgccgcc cgcaccgggg gcggcctggg
    10621 ggctacatcg cgataccggt tttttcgagg atgtcgctga ccagttccag ccgcaccagc
    10681 gggttgtcca gctccatcag gcgctgcttg agctccagcg gcatgggcag cagttcgcac
    10741 cagcggttgg ccacccagcc gcagtcgtcg agccggtagg gcggctccag cggcatctgg
    10801 tccgctggca cgccgcgctc ctgcaggctg cggatcagct tgcccagcgc attggccgtg
    10861 ccgcgcaagt cttcgggtac cgggatggcc tggtcgtcgt ccagccgcgt cacgtcggcg
    10921 atccacaggc cgtgtttgag cttttcgcgg ctggaaatct cgaaacgctg cgtgccgctg
    10981 caacgaatca ccatcaggcc cggttgcggc accgaaaagc tggtgatggt ggccagcgtg
    11041 ccgacgttgt agaaggcctc gctggcaaaa ccatcgccgc tgggcccgtc ctcgggcttg
    11101 cgcacttcgg agccctgggt cagcgccacc acgccgaagg gcgcgccggt cttgtggcat
    11161 ttgccgatca tgtcgaggta acgcacctcg aaaatccgca gcggcagcag gccaccgggg
    11221 taaagcaccg tgcccagcgg gaacagcggc agcgaggaga gggtgagcac gtcagacatc
    11281 gatcaggcgg gggaatttct gtgaagcact atcttcccac gatcctgttt ccccatgtgc
    11341 gggctttggt gcccgccggg ctattcgtgc caagtggttt gccgacgggt agacagggcc
    11401 cggaccggct gggcgcgttg tcattcatga aaacagcctc cagccctttt ccacagggcg
    11461 ccggcagcta tcaatttgag agtgaagccc tgccgggccg acgcccgtgg ctgccgtggc
    11521 ctcaggcgac ggcgtccgcc ggctggcgca gcagcatggc cagtgagttc gccacggtct
    11581 tgcgcagttc acgccggtcg cagatgaaat ccaccgcccc cttggtctgc aggaactcgg
    11641 cgcgctggaa gccttcgggc agggtcacgc gcacggtgga ctcgatgaca cgcgggccgg
    11701 caaaaccgat cagcgctttc ggttcggcaa tcacgatgtc gccgacaaac gcgaagccgg
    11761 cactcacccc gcccatggtc gggtcggtca gcacgctgat gtagggcagg ccttttttgg
    11821 ccaggcgggt cagcgaggca ttggtcttgg ccatctgcat caggctcagc aggccctcct
    11881 gcatgcgtgc gccgccggtg gcggtaaagc agatgaaggg cactttctgc tcgatcgcgg
    11941 catgcacacc acgcacaaaa cgctcgccga ccacgctgcc catgctgccg cccatgaagt
    12001 cgaactcgaa gcaggccacc accacgccga tgctgtgcac cgcgccgccc atgacgatca
    12061 gggcgtcggt ttcgccggtg ttttccagcg cttctttcag gcgctcgggg tatttgcggc
    12121 tgtccttgaa cttgagcgga tcgaccggca gcacttcctg cccgatttca taacggcctt
    12181 ccgggtccag aaacgagtcc agccgggccc gcgcgtccat gcggtggtgg tggctgcact
    12241 gcgggcagac gttctggttt ttttccaggt ccgtcttgta gagcacggct tcacagctgg
    12301 ggcacttgac ccacaggcct tcgggcacgc tgcggcgctc ggtcgggttg gtcgggctga
    12361 ttttcggcgg gaggagtttt tctagccaag acatgatggg tccttatgtc cttgtggggt
    12421 ggtgcccccg gacggcgcag gccgctcgca ggggtaaaac cggaaaagaa aggggattat
    12481 gcgctgtctc cgggcccggc cggcgcgtcc agcgcctgcc ggacatcggc caggaaatca
    12541 cgcaccaggg gcaccacttg cgcgcgcggc tggtcggtga tcagctggat gatgcggctg
    12601 ccgatgacca ccgcgtcggc gacccggccg atggccctgg ccgtggcggc gtcgcggatg
    12661 ccgaagccca caccgaccgg gatgctgacg tgttggcgta tgcgcggcag catggcctcc
    12721 accgcaccca ggtccagcgt gccggccccg gtcacgccct tgagcgacac gtagtacaca
    12781 tacccactgg ccacctgggc gacctgcgcc atgcgttccg gcgtggaggt tggcgccagc
    12841 aggaagatca ggtccatctc gtgggcgcgc agggtggcgg caaaggccac acattcttcg
    12901 ggcgggtagt ccaccaccag cacgccgtcc acaccggccg ccgaggcgtc gcgcacaaag
    12961 gcgtccttgc cgcgtttgag gtcataggct tcgatcgggt tggcgtaacc catcagcacc
    13021 accggtgtct tgctgtcttt ctggcgaaag aggcgaacca tctccagcac ctgcgccagg
    13081 ccaatgccca tggcgagcgc ctggtcgccg gctttctgga tcaccgggcc gtcggcactc
    13141 ggatcggaaa agggcacgcc aagctcaatg acgtctgcgc cgccggccac catgccgtgc
    13201 atgagatcgg gcgtgatgtc ggcaaacggg aagccgcccg tgacgtaggg gatcaaagcc
    13261 ttgcggtttt gcgccttgag cgcttcaaaa gttgtggcaa tacggctcat gatggtgttc
    13321 cacttccctt gacggtgtgg ccgcgcatcg agggccggtc gtaaaaatcc gcgcccgaca
    13381 ggtcggccac ggtgccgatg tccttgtcac cacggcctga caggttgacc aggatggact
    13441 ggtcgctgcg catggtactg gcgagtttca tcgcgtaggc cacggcgtgg ctggactcca
    13501 gcgcgggaat gatgccttcg gtgcggcaca ggtaatgaaa ggccgacagc gcttccttgt
    13561 cggtgatgcc tacatattcg gcgcggccga tgtctttcag aaacgcgtgt tcggggccga
    13621 caccggggta atccaggccg gcgctgatgc tgtgggtctc ggtgacctgg ccgtcctcgt
    13681 tctgcagcac ataggtgcgg ttgccatgca gcacgcccgg gctgccgcgc tgcagcgagg
    13741 ccgagtgttt gccgctgtcc atgccttcac cggccgcctc gacgccgatc aggcgtgttt
    13801 tctcatgcga gatatagggg tggaaaatgc ccatggcgtt gctgccgccg ccgacacagg
    13861 caatcaccgc gtcaggctgt ttgccgctgg tcatttccgg catctgggtc aggcactcct
    13921 cgccgatcac gctctggaaa tcgcgcacca tcatcgggta ggggtgcggg ccggcgaccg
    13981 tgccaatgat gtagaaggtg ttgtccacat tggcgaccca gtcgcgcatg gcttcgttga
    14041 gtgcgtcttt cagggtcttg ctgcctgact cgaccggcac cacggtggcg cccagcagct
    14101 tcatgcggta gacgttgggg ctctggcgct tgacgtcctc gctgcccatg tagaccacac
    14161 attcgaggcc gtaacgcgcg cagatggtgg ccgtggccac gccgtgctgg ccggcgccgg
    14221 tttcggcgat gatgcgcggc ttgcccatgc gccgggccag catggcctgg ccgatggtgt
    14281 tgttgatctt gtgcgcgccg gtgtggttga ggtcttcgcg cttgaggtag atctgcgcgc
    14341 caccctgctc gcggctcatg cgggcggcat ggtagatcgg ggaggggcgg ccgacgaaat
    14401 gcttgagctc gtagtggaat tccgcgagga aggccgggtc gtgctggtat tttgcgtaag
    14461 cgtctttcag ctcattgatc gcatgggtca gcgtttcaga gacgaaactg ccgccgtaaa
    14521 tcccgaagtg accggttggg tcaggttgct ggtaggtgta catggttttc tgcaagtaag
    14581 tcgtcggcgg cacgaacggc tgcgacgaac tgatggatct tgtcggcgct tttgattccc
    14641 ttgagggtgg cccctccggt agcggaaatc tcgacgcccg aactgacatc gacggccagc
    14701 gtcttacaac gcggccgcac ttgcaaaatg ccatcggtca catttgcagg tgtgagtcca
    14761 ccagacaaaa cgaggtgagc gttgacgctt ggaggaagaa gtgaccaatt gaatgttttt
    14821 ccgccgcccc cgaatccgtc gacatgggcg tcgagcagga tggcctgggc ggccttgaag
    14881 tcttccgcgt attttacgag atcaaaagcg gcactcccct cggcggggtt ttcctccagg
    14941 ggaatacgtg ccgcgcgcag gaagggacgc ccggcccgca ggcaggtttc cggcgtttca
    15001 tcaccatgaa attgaagtaa agccattggt acacgggcgc aggcagctat gatttcagga
    15061 gcatctgcat tgacgaaaag caggaccggg gtgacaaaag gcggcaggcg gcgggccagc
    15121 tcggcggcgc gttcagcgct gacgctgcgc gggctgggcg cgtacatgac aaaccccacg
    15181 gcatcggcgc cggcggccac ggcggcgtcc acgtcctctt cacgcgtgag gccgcagatc
    15241 ttgatacgag tcctgtgcat ggtcatggca accaatcata agccgcggtg cgctcgggca
    15301 ggccgtaacc agggtcatac cggggcccca ggaagtacag gccgtcgggc gaaaacgtgg
    15361 gggcggccac gtcccggcgg cgtgcggcca gcacctcggc gagccagtcg ggtggatgct
    15421 ggccctgtcc gacggcgacc aggcaaccca tgatgttgcg gatcatgtgg tgcaaaaagg
    15481 cattcgcttc aaactcgaag cgccagtagc ctgcccgttg cgaaatctcg atgcggctga
    15541 tggttttgac cggcgacttc gcctggcact gtgccgcgcg gaaggagctg aagtcgtgtt
    15601 cacccagcag gtggacgatg gctgcgcgta tggctgccgc gtccatcggc cggtagaccc
    15661 agccgacacg gccgacctcg acactggggc gcaccggcga ctccagcaac acgtaggcat
    15721 agcgacggct gattgcgctg ccgcggcaat gaaactcggc aggcacctca cgcgcccatt
    15781 gcacggcgat atcgcggggc aaaaaagcgt tggtgccgcg cacccaggac ggcagctcgc
    15841 gctgcaagga ggtgtcgaaa tgaaccacct gcatcagggc gtgaaccccg gcgtcggtgc
    15901 gcccggcaca cagggtgcgc aaaggctggg cggcgaactt gcccagcgcc ttttccagcc
    15961 ggtcctgcac cgtgttgccc gagagctggc tttgccagcc ctcgtaagcg gtgccgctgt
    16021 agctcagtcc cagagcgatc ctcacgggca gccctcaggg gaaaggtgca aggggtcagg
    16081 ccagggcgtt aagaaatgcc tgggccttgc ttttgagcga gccgctcgat tgcgccagca
    16141 cttcctcggc cagcgagcgt gcgccatcga catcgccaat cgcgcggaac tcttcggcca
    16201 gtgcaaactt ggtttccagc gggtcgtcac tgtctaccga cggcgtgagc tcgctggcgg
    16261 cggtgatttt ctggctttcc gtggtcgggc tgtcgagatc gagcgacagg gcgcccatat
    16321 caaactcaag catgccgcca gaggtgtcca gatctgcggc acctgtgttc acggtggccg
    16381 cttcggtgga gaagtccagg ctgttccctg ctacgggcga ggcgacgggg gcatcggctt
    16441 tcgccggagc cggtgccggt gccgcagcag gcatggacac tgttgcggaa ctgaagtcca
    16501 ggctgttgtc ggcgggcgcg ggcgccgcag ccggcatggc cagaacgggt tgaaccggtg
    16561 ccacggggac cggcgtcgca acggctacgg gtgcggcggg ttcgtcgtcc agcgagaaat
    16621 caaggtccag gtcgaaatcc acggccgcag ccgccggctc tggcgccggg ctggcggcct
    16681 gggccacagt cggtgcggtc aatcctgcgg cggtggcgcc ggcaacggcc atgctgctgc
    16741 ttgcggctgt ggccccaggt tgtccaccgg gttggtacat ggggttggca gggtccagct
    16801 cacggcccat ttcagccatg tgcgcccatt caacaccctg gccccgggtc aggttgaacg
    16861 cttcgttcgc caccatttca aaagccttgg tgtcgcggcg tttggcgtag atctccatca
    16921 gcttcgcatg gatggccaca cgtgtggggg tcacacggat ggcttccttg aggatttcct
    16981 cggcttggag gtcacgaccg taagccaggt aaacatcggc ttcagccacc ggatccacat
    17041 cgccggcggc atcgagctgg ctcggtgaat acaccatcga cgagcctgtg acgttggcct
    17101 cattggtgtc tatgcgctgg ccgccgctgg cgccgaagaa ggaatcgggc tgcaggcgac
    17161 tttcaaggaa ggagctgtcg accgagacag catttttgcg ctgacgggag cggtacacgc
    17221 cgaaggctgc cagaagcacc agcagggcag cgccgccagc cagcatcagc gggttgtcga
    17281 gcagttcgtc gatcaggctc ggctccggtg gcggaggagg aatcacgacc ggttttttca
    17341 ccggcgccgg ggccgctgca atcactgggg cggtggcgct ggcagtggct gcgggtggca
    17401 cggccgcgct ggcgggactc gcggcagcgg cggaagccgc agggactgca gcagacaatg
    17461 cgccgggtgc agcggatacg gccgccggtg ttgccacggc cagtcccggc gctgcagggg
    17521 tggttgccgc aggagcaacg ggaggcgtgg cgggcgtcgc cggagcggct ggcgctgcag
    17581 gcgcactggt cagcttgttc aggtcgctga tatttttcga cagctcggcc acgcgtgcgg
    17641 ccgcttcctt ggcctggcgt tcctgcgcga ttttttcttc ggccgctgcc ttgctctgga
    17701 ccgcaccttt ggagagggtc agtttgtcag gcgttgcggc ggcaggcgtc ttgtcctcga
    17761 ccttggcctg gacctggcct gcggccttgc ggtctgcacc ggcaacctgg gtcgtcgggg
    17821 cgccttcggc cagtttgcgg cggaactcgt tgaagtcctt gctctgcgca acaatggtcc
    17881 gggtggcctc gccggcgggg atgtattccg cctggtcgcc agccgggata tccagcacgg
    17941 caccggcttt gaggcggttg acgttgccgc ctatgaaggc atcggggttg ctgcgcagca
    18001 gcgcgaccag catctggtcc agcgacacgc tggccggttt ggcctgcgcc gcaatttttc
    18061 cggctgtatc gccaggtttg acaacaattt tctggccatc cgcggaggct ggccgttcgg
    18121 cgggtgccgg ctgcggcgtg ggcgccttgg ccaccggggc cggcctggac ggaggggccg
    18181 acggtgtcgc cgcgacggcc ggccgcggtg gagccggcaa ggctgccacc ggaggcgggg
    18241 cggcaggccg cgaaatgacg ggtgcggtcg gggccggcat ggcaggcgcg gcggcctggc
    18301 gcagattcgg tggatcgaac agcatcgtgt agtcacgcac gatgcgtccg gatgcccaac
    18361 gggcttctag gatcaggtcg acaaaaggtt cgctgacggg cctgtcgctg ctcaggcgca
    18421 ggaaagaccg gccatcggtg cggcgctgca ggctgatctg gacattggca actgcggcgg
    18481 tgtattccag tccggcggcc ttgaaggcat cggcattggc gacgctggcc cgcagggatg
    18541 ctgcttcgtc ggcactgatt tcgacgattt cgatttctgc ccgcagtggt tcgcccaagg
    18601 cggactggac gatgacccgg cccagcccca aagcatgtgc cggcaaggtg gcgatgctac
    18661 ccaaaacagc catggcagcc gccacggctc ccatgcgcca acggacgggt tgattcatgg
    18721 gaattggagc ctccggtgcc cgggttgcat taaattttat tctgctggtc cgcacaaaaa
    18781 cctcaacgct aattttcgac aaaacaacat taacatcaag gcattaggct gacaagctaa
    18841 gacagcactt aagttttgac acccggggtt agcatcaact tacaacttcc agatgttgcc
    18901 tgacgacaac gggccgtcac aagtggttac ggatggtggc cgggcatcac cagccactcc
    18961 ctcctgattt caggcctgca gcaggatgcg cagcatgcgg cgcaagggct ctgccgcacc
    19021 ccacaacaac tggtcgccga ccgtgaaggc gcccaggtaa tccggcccca tggccagctt
    19081 gcgcagacgg ccgaccggaa tggtcatggt gccggtcacg gcgaccggtg tcaggtcggt
    19141 gatgctggct tcgcgggtgt tgggtaccac cctgacccac tcgttgtcgg ccgcgatcat
    19201 ggcctcgatg tcggccagcg gcacgtcttt cttgagcttg aaggtcagcg cctggctgtg
    19261 gcaacgcatg gcgccgatgc gcacgcaaaa accatctacc ggcgtttcgg cggtgccgaa
    19321 gctggcgccc tggcccagga ttttgttggt ttcggcgccg cccttccatt cttccttgct
    19381 catgccgttg ccgaggtcct tgtcgatcca ggggatcagg ctgccgccca gcggcacgcc
    19441 gaagttcgca gtttctgcgg acgacagcga ttgctgtttg gccagcacgc ggcggtcgat
    19501 ttcgagaatg gccgatttcg ggtcgtccag cagcgcccgg acttcaccgt tgagtgtgcc
    19561 gaactgggtc agcaactcac gcatgtgctg tgcaccgccg ccggaggcgg cctgataggt
    19621 catggaagtc atccactcga ccaggccggc cttgtacagc gcgcccacac ccatgagcat
    19681 gcaactgacc gtgcagttgc cgccaatcca gttcttgccg cccttggcca gcgcgttctg
    19741 gatcaccggc aggttgaccg ggtccagcac gatgatggcg tcgtcgttca tgcgcagggt
    19801 cgatg
//
LOCUS       DAIDFG010000015        19453 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_15_length_19453_cov_16.669502, whole genome shotgun sequence.
ACCESSION   DAIDFG010000015 DAIDFG010000000
VERSION     DAIDFG010000015.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 19453)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19453)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19453
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15_length_19453_cov_16.669502"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3293 a         6040 c         6651 g         3469 t
ORIGIN      
        1 atccgtgagt cctgcctgat tgatgcgatg aatgcagatg acggattgaa ccgcgatatt
       61 ggcgagctgg cgcgtctgct gcgtgtgctc tccggcatgt atgaccaggc tgcgcgggct
      121 gcagcctccc tttaagaaac catcaaggta acgaacatgg caaccgaaac ggcaacggca
      181 atggcaatgg aaaagaagcg ctggcagttc tggatagacc gcggcggcac attcaccgac
      241 atcgtggcca aacgccccga cggttcgctg gtcacgcaca agctgctgtc ggagaacccc
      301 gagcagtacc gcgatgccgc cgtggccggc atccgccacc ttctgggcct gaaggcgggc
      361 gagccgattc gcgccgatgt ggtcgactgc gtgaagatgg gtaccacggt ggccaccaat
      421 gccttgcttg aacgcaaggg tgagccaacc ctgctggtga cgacgcgcgg ctttcgcgac
      481 gcgctgcgca tcgcctacca gaaccggccg cggctgtttg accgccacat cgtgctgccc
      541 gagctgctct acagcgccgt ggtggaagcg cacgagcggg tcggggccca gggtgatgtg
      601 ctgcaggcgc tcgacgaaac gctattaaag caggagctgc tggcgcagta ccagaaaggg
      661 ctgcgcagcg ttgcgattgt tttcatgcac ggctaccgct acaccgacca tgaaaaggcg
      721 gcaaagcgga ttgcgcagga cgtgggtttc acgcagatca gcacctcgca cgagaccagc
      781 ccgatgatga aattcgtcag ccgcggggat acgaccgtgg tcgatgccta cctctcgccc
      841 atcctgcgcc gctatgtaga gcaggtcgcc ggcgagatgc ccggagtcaa gctgtttttc
      901 atgcagtctt cggggggact gaccgacgcc catgtgttcc agggcaagga cgcgatcctg
      961 agcgggccgg caggcggaat tgtcggcatg gcccgcaccg cggcgatagc cggccatacc
     1021 aaggtgatcg gcttcgacat gggcggcacc tccaccgatg tctcgcacta cgccggggag
     1081 ttcgagcgcg agtttgaaac ccaggtcgcc ggcgtgcgca tgcgcgcacc catgatgagc
     1141 attcacaccg tggcggccgg cggcggctcg ctgctgaagt tcgacggcga gcgcttccgc
     1201 gtcgggccgc agagcgccgg tgccaacccc ggcccggcca gctatcgccg cggcggccgg
     1261 cttgccgtga ccgatgccaa cgtgatggtg ggcaaggtgc agccgcgtta ttttcccaag
     1321 gtgttcggcc cgaatgccga tgaagcgctc agttacgagg cggtggaaac gcagttcaac
     1381 gaactggccg gccagaccgg gcgcagcgcc gaggtcgtgg ccgaaggttt catcaacatc
     1441 gcggtgcagc agatggccaa tgccatcaag aagatctctg tcgcccgcgg gtacgacgtg
     1501 acgcgctaca ccctgcagtg tttcggcggc gccggcgggc agcacgcctg cctggtggcc
     1561 gacgcgctgg gcatgaccag ggtgtttgtg cacccgctcg ccggcgtgct cagcgcctac
     1621 ggcatgggtc tggctgacca gaacgtgatc cgtgaacaag cggtggagct caagctgact
     1681 gagtcagctg tgcccgaaat tgccgcaacg ctcgaaggcc ttgcaacgac ggcgcaggtc
     1741 gaactccagc gccagcaggt cagcctgggg cccatcacca tgcaccgccg cgtgcatgtg
     1801 cgctacgagg gcagcgactc ggcgctggtg gttcccttcg gcacgctgga ccagatcgag
     1861 gcgagttttg aagccgccta ccgacagcgt tttgcctttc tgatgcaggg caagggtctg
     1921 gtggtcgaag ccgtgtcggt tgaggctgtt gtcgctggcg atgcgccggc tgagccacgc
     1981 caccacacgc acgaaccacg tgaagtgccg cgccgggaaa cggtacgcat gtattcgggc
     2041 ggccagtggc acgatgccgc gctggtggtg cgtgaggatc ttcagccggg cgacattatt
     2101 tccggcccgg ccatcattgc cgagaaaaac gcgaccaccg tggtcgaacc cggctgggag
     2161 gccgcgctga cggcactgga ccatctggtg ctggaccggc gcgcggagcg tgcgatcaag
     2221 tttgcagccg gcacgacggt ggatccggtg ctgctggagg tattcaacaa cctgttcatg
     2281 aacatcgccg agcagatggg cctgcagttg cagaacaccg cctactcggt caacatcaag
     2341 gagcggctgg acttcagctg cgcgttgttc gacaccgaag gcaacctgat tgccaatgcg
     2401 ccgcacatgc cggtgcacct gggttccatg ggtgaaagca tcaagacggt gatccgcgag
     2461 aacgccggaa aaatgcagcc gggcgacgtc tatgtgctca acgaccccta tcacggcggc
     2521 acgcacctgc ccgacatcac ggtgatcacg ccggtctacc tgggcaacgc ggacctgccg
     2581 accttttatg tcggctcgcg cggtcaccat gccgacatcg gcggcaccac gccgggttcc
     2641 atgccgccgt tttcgacacg catcgaggaa gagggcgtgc agatcaacaa cgtcaagctg
     2701 gtcgaacgcg gtgtgctgcg cgaagccgag atggtggcgc tgctgaaaag cggtcaatac
     2761 ccttcgagga acccgcagca gaacatggcc gatctgaagg cgcaggtcgc cgccaatgaa
     2821 aaaggcgtgc aggaactgcg caagatggtg gacaccttca gcctggaggt ggtgctggcc
     2881 tacatgcgcc atgtgcagga caacgccgag gagtcggtgc gccgcgtgat cacccggctg
     2941 aaggacggcg ctttcacgct gccgctggac aacggcgcgc agatccaggt ggcgatccgg
     3001 gtggacacga aaaaccgcag cgccgagatc gacttcaccg gcacctcgcc ccagcaaagc
     3061 aacaacttca atgcccccac ggcggtgtgt atggcggcgg tgctctacgt gttccgcacg
     3121 ctggtggacg acgacattcc gctcaacgcg ggctgcctga aaccgctcaa ggtcatcatc
     3181 ccggccggct ccatgctgaa cccgaaccca ccggcctcgg tggtggcggg caacgtggaa
     3241 acctctacct gcatcaccaa tgcgctgtat ggcgcactcg gcgtgatggc cgccagccag
     3301 tgcaccatga acaacttcac cttcggcaac gagcggcacc agtattacga aaccatctcg
     3361 ggcggcagcg gtgctggcga tggcttcgac ggcacgtcgg tggtgcagac ccacatgacc
     3421 aactcgcgcc tgaccgaccc cgaggtgctg gagttccgct tcccggtgcg gctggagagt
     3481 tacgagatcc gccagggctc gggtggcgcc gggcgctggc acggcggcaa cggcggcgtg
     3541 cggcgcgtgc gttttctcga acccatgacc gcgagcattt tgtcgaacgg ccgcaaacac
     3601 ggcgcgtttg gcatggccgg gggtgagccg ggccaggtcg gccgcaatgt ggtggtgcgc
     3661 gccggcgggc agatcgaggc gctggagcac atcggtcagg cagagatgtt gcccggtgac
     3721 atctttgaga tccacacgcc gggcggcggc ggcttcggca agccatgagg cgaggcttca
     3781 tttgctgatg tgtggtaaaa aacgctttcg gcccttattc cacgggcgca ggcagctatc
     3841 aattcaggag tgacgggcat ggatgcggca tttgcggaca actaccgctt tctggcaaag
     3901 tacaaccgct ggatgaacca gcgcctgttc gacgcctgcg ataccctggg cgacgaagaa
     3961 cgaaaacgcg agcgcggtgc ttttttcgga tcccttcacc gcacgctcaa tcacctggtg
     4021 gtggctgacc agatctggct gcggcgtttt gcgcagtgcg gcctggacca cggcgtcacg
     4081 ttcgccagcc tgaacggggc cgtgctggat ctgccggcgg gcagccggct ggacaccgtg
     4141 ccgtttgacg actggcccgc cttgcgcgcc aagcgcgagc agctcgatgc cgcgatcgag
     4201 gcctgggtgg cggacatgcc cgagggctat ccgcagttca ccatgaccta cagcaacagc
     4261 aagggcgtgc cgcgcgccca cccggcctgg caggcgatga cgcatttctt caaccaccag
     4321 acgcaccacc gcgggcaggc gaccacgctg ctgatgcagg ccggcgtgga cgtgggggtg
     4381 acggacttga ttgcgctggt gtaaggcggg gctgcgtcca cgccggtggc gtctgccgtc
     4441 gttacccgtg ctttcatggc cttctgacgt ggcgttgtcc cgcttcgaag ttccttcact
     4501 tcgtggcaag acccctcaca tcgcccgcgt cagatacacc gcttcgcgcc cgacaatcgg
     4561 ctcccgggtc ggcatgaaca ccgtgcagcc gtcgcacggt gaacgcactt cttccgcatt
     4621 gccgtcggtg gcgatcagct cgcccttgtg gaaggtctca aaaccgagca ggggcttgac
     4681 gaagcggaac tcgctcgtca gcaccatgcg ggtttgcagc agctcgaagc ggcgttgcgc
     4741 gggcagggcg gaaggcgcgg cggtgtgttc gatcaggccg aagtgggcca ggaagtccag
     4801 cgtcacctgg gtcgccacat cggccgagct ttgggcgaag tgctggccgc attccaccac
     4861 cagcgccgcg ccggcgttgc tgccctgttg gccatggcga ccgtgctgga tcaggggggt
     4921 gcctgagccc aaaccgtccg gcatcaccag gtggacagcc ggcctgccta tggcttgcgc
     4981 aacggccgcg ttgcgctcga aggcggggta gacccagaag ggttccacgg cctgcgaggt
     5041 cgagtggatg tccaggatgt ggtcggccgc tgcaatcacc gggcgcaact cgcgtgcgcg
     5101 gcgcagctcg ggactgtctt cggggccgtc cagccactcg tccgaccaga cgcggttgag
     5161 gttgtgaacc acctggcggt tgtcgtaggg ctggtcgggg ttgaatgcgt tgtaggcctc
     5221 gatatgcgca aagctgacgg tgagcgtgcc gatcagcggc cggatgccgg catccagcag
     5281 atgcgtggcc gcgaccatgc cgcagatctc gttgccgtgg gtcagtgcgt tgaccagcag
     5341 gtgcgggccg ggccggcccg aggcgaagcg gtgaacgtag tcgatgccgg tgttgcccgc
     5401 ccggtaggcg gacaggtcgc ggggaagaac ttcgaggggc agttcagagg tcaatggagt
     5461 ccttcggtgg tggaggtggg tggcgccttg cgtcaccaaa tgctaaataa ataatagctg
     5521 aatatcgcgc atgggctgac gcgacggggc tgcttttcat caggaatggc ttgttgcgca
     5581 ggcccggtgc gaccgccggg gtactccccg gcctggggtc aaacacctcg gggcaggcag
     5641 actgcaggac gattgttgtc gaggtcagaa acagggcgac ggcgatcggt agctaccccg
     5701 tggtttgacg caaatcagca gcagccagcc ggcccgcaaa cgctggcgat gctgcccggc
     5761 gcgccgtcaa acggcaggct ggtgccgcag ccttcgaaaa tgccatagtg cgtgtcgaag
     5821 ttgccgatga agctgaagtg cggcgccagg cgcgtgccct gcagcatgtg ccaggtgttg
     5881 ccgcagaccg ggaacacctt gccggtctgg atgtcgtggt gtttgtcgag cacaaagcgg
     5941 cccgtctggc cggggatgct tccctggtaa atcaccgcct ggccatagtc ttcacaagcg
     6001 gtctccagcg cggccagctt gaacagccgc caggtggccg aataaaagcg caagttgccg
     6061 gtgcgcgccg caagttgcgg gtcggtgatg tccagcggcc ggtcttccac caggcgcggg
     6121 tcggcaaagc cgtggccttg cgccaggcgt tcgaagtctt tccagtacag tgcgccgccc
     6181 aggcattcgc catacagcac cgggtcgttt ttgaccgcgg ccggcacgcg gcgatcggca
     6241 tacacatcgg aaaagtagaa ctcgcccccg ggcttgagca ggcgctgcac gccggcgagc
     6301 accgcgtcct tgtcgggtga gaggttgacc acgcagttgg agacgatcac gtcgaagctg
     6361 ccgggcgcca ggccgagttc gtccagccgt tcgatgtagc cgtgcagaaa cgcgacattg
     6421 tcgtagccga acagcgccgc atggtgggcc tggtgcgtgc gcgccacggc cagttgttca
     6481 tcggtcatgt ccacgccgac caccgagccg gtgggcccga ccagttgtgc cagcgcatac
     6541 acgtcgcggc cggagccgca gccgaggtcg agcacgcggc agccctccag cagcggcggg
     6601 cagaccaggc cgcagccgta ataacgcgac agcacctcgg gatggatgcg cgccagcagc
     6661 ggcttgagcc actcgggcat gctgctgacg tcgcagcagg cgctggtctt gaggtctgcc
     6721 gtgccctgca gctggcggcc gtagtagtcg gtgacgatgt cgtaggtgtt gctgttgttg
     6781 tcgttgctca tgggcgttct tccggaaatg aatttgctat gaaatcagga gctggtggcg
     6841 cttgtactga ttgggctgga ggccgatttg gtttgatgtt ttggaccacg aagccgaccg
     6901 gcaggtgggc caggtccgcc gcttcgtcga tgtcgtgcag caccggcccc tgccagacgc
     6961 gcaggcccag cgcgctcagg cgctgcagtg tttcggcagc gacggtggat gtgctccacg
     7021 ccatctgcct gaagatggcg gggcagggcg ctcgcaggcc gatcagcaca taaccgccgt
     7081 cggccaccgg cagcagcacc gcgtcgtgtt gcgccagttg ccgggccgcc tcgcgcaggt
     7141 gcgcgctgcc aagtgccgga cagtcggtgc cgatcagcag caccgggccg gggtgcaggg
     7201 cgagggcgcg ctgtatcgca cggtccatgc gttcaccgag gtcgccgtcg ccctgcgccg
     7261 tgtagtccac accttcaggc agcgccacgc cctgccaggc caagtcaccg ggcgccgggc
     7321 tcatacacag ctccaccgcg tccaggcccg ccgccagggc ctgctgcagc gtgtgcgcca
     7381 gcatggcacg cgccagcgcc gctgcgccgt ccgcacccag cgccgggatc agccgcgtct
     7441 tgacggcgcc agactggggc gccttggcaa aaatcaccag gcgggtcatg gcgcgttcac
     7501 cggtagaggt ccgcaagccg ctcgggcgct gcgccgcgcc agtaggcaaa acgcaagcgc
     7561 cacatcagca gcacggttcg ccagacgccg cggctttccc agcgccggcc cgaggtctgc
     7621 actttggcgt tcaggcaggc cgggcgcgac agtttgagca ggcgccggga catctcgatg
     7681 tcttccatca gcggctgggc cgggaagccg cccacggcct cgaaggccgc gcgcgtcatg
     7741 aacatcgcct ggtcgccggt ggcgatgccg gtcagccgcg agcgcaggtt catcagggcg
     7801 gcgatcagtt tgagcatggg ggggcggccg gtgatggcca catcaaaacg gccccagacg
     7861 cgcgagccgc cggccagggc ttcgctgatc agcgcgtcgg cgccgtcggg cagcacggtg
     7921 tccgcgtgca gaaacagcaa gacatcgcca ctggcctgcg cggcccccgc gttcatctgc
     7981 agcgcccggc cgcgcggcgc gtggacaagc tgtgcgccgc cggctgtcgc cagcgcggcg
     8041 ctgcggtcgc tactgccgcc gtcggccacg atgagctcgg cgccgcgtgc ccgcagcggt
     8101 gccagcgcat gcagggcgtc acccaggccc gcggcttcgt tgagcacggg caaaaccacg
     8161 gataggcgcg ccgctgccat ggcggctcag tgctccagcg cgccgccgca actgctgccc
     8221 tggccggcgg tgcagccata acagtggccc gccacacgga tgggctgctc atcgatcccg
     8281 ctgccgagca ggtcacgcag atggcgctgc aggcccgagg tgcccagcgg caggccgagc
     8341 tgctggttga agtcgcagtc cgacagccag ccctgccagt ccacgctgac ggtgttgcgg
     8401 cacatcacgc cggccaggtt ctgttcctga aagctgccct tgagcaggtc catgtagttg
     8461 tcaaacgtgc ccttggacac cagggtcgag ccaaagcgct ggatgggcat gttggtcagc
     8521 gcatacagct cgttgaacac aatgccgaaa tgcgccagca actcgcgctt gtagtcggcc
     8581 tgcagcgcct gctggtcggg cggcagcgac gggccctgtg ggttgtagac caggttcagc
     8641 gacaggccgg aaccggcctg gccgtagccg agccggttga gtttttgcaa ggccgcgatg
     8701 ctgaggtcga acacaccttc accgcgctgc ttgtcgacat tggcggccga gtagcagggc
     8761 atggaggcca cgatgtccac gccttgctct gccaggaatt cggccagccc ttcctgcccg
     8821 ggctcgagca ggatggtcag gttgcagcgg tcgatcacac gcacaccctg cgcgcgcgca
     8881 gtgcgcacca gctcgcgaaa gccctcatgc agttcgggcg cgccgccggt caggtccagc
     8941 gtgcccaggc cgcgcgcgac cagcacctgc gggatcagtg ccagggtgtc cgcatccatc
     9001 atttcggtgc ggttcggacc ggcgttgaca tggcaatgca ggcagctctg gttgcattgg
     9061 tagccgaggt tgacctgcaa ggtgtcgagc ttgccgcggc ggatggccgg gaagggcgtt
     9121 tttttcaaca aggggagggt gtcgtgcata agcggatgtc catcaaaagt cagggcagca
     9181 acgcggccag gcgtgcgcgc acctcggggg ccatggcgtc gggctgggtg accagcgcgt
     9241 tgtgcaaggc ggtgtgggcc tcgctggccg gttgctcggc tcccagcagg cgtatcgcct
     9301 cggccaccac ctgctgggcg cggcccgcat ttttcagcag gttggccagc gccatgttgg
     9361 cgttcacctg cgcctcgcgc gggtgccagc aatcgaaatc ggtgaccatg gccagtgtgg
     9421 tgtaggcgat ctgtgcctcg cgggcgagtt tggcctcggg catattggtc atgccgatca
     9481 cgcccgcgcc catgctgcgg taccaatggg attcggccag gctggaaaac tgcggcccct
     9541 cgatacacac ataggtgccg ccttcgtgca gggtgacgcc gtggccggag gcatcggcag
     9601 ccagcacgct gcgcaccgca cgcgccagca cgccggcggc agcggggcag accggctggg
     9661 ccatggagac atgggcgacc gcgccctggc cgaagaaagt gctgtcccgg cgcttggtca
     9721 ggtcgatgaa ctggtcgggc agcaccatgt ccagcggctt gaaatcctcg cgcagcgaac
     9781 ccacggccga caccgagacg atgtaacgca cgccgagctg cttcatggcg tggatgttgg
     9841 cgcggtacgg aacttcgaag ggcagcaggc ggtggccacg cgcatgacgg gccaggaaca
     9901 cggctggaac gccgtggacg ctgccggtga ccagcacgtc ggagggcgcg ccgaagggcg
     9961 tgtccatcac atgctcttgc gcgtcctgca acgcgtccat ctggtagagg ccgctgcctc
    10021 cgatgatgcc taccagtgcc tgggtcatgt gggggatttc cttggggatg ggtgaatgtc
    10081 aggctcagac agggattttg agcggatttt gaggaagcag tgttagcctc gcgttaaacc
    10141 cattttcctg cttggtcgct gccgggcggg atttcttaca ccgcccacgc ctggttttgt
    10201 aagaatcggc gttttcgccc gacaacatct acagaacaca caccggggac ggacttgaat
    10261 tcacgcaaac ttgtcattgc cgtgctgctg ctggcgggca taggcgcctt ttttgcgctg
    10321 gatctgggcc gttatttgag cctggctttc ctcaagcaga gccagatgtc tttccaggcg
    10381 gtttttgagc agaagccggt gtgggtgacg ctggtgtttt ttgcggttta cgttgccgtc
    10441 accggcttgt cgctgccggg cgccgtcatc atgacactgg cggctggcgc cggttttggt
    10501 ctggtgctcg ggaccatcgt ggtgtctttc gcctcgaccc tgggggcgac gctggccatg
    10561 ctggcggcgc gttacctgct gcgggacagc atccaggccc gttttggcaa gcggctggac
    10621 gagatcaaca agggcataaa gaaagagggt gcgttttacc tgttctcgct gcggctgatc
    10681 ccggtcgtgc cgttttttgc gctcaacctg ctcatggggc tgacccgcat cagaacctgg
    10741 acctatttct gggtcagcca gctcggcatg ctggccggcg ccgtggtgta tgtgaatgcc
    10801 ggcacgcaga tcgcgaagat cgattcgctg cagtcgattg cctcgccggc gctgatcggc
    10861 tcctttgtgc tgcttggtct gttgccactg gtcgtcaaca agctgctgca gtttctcaag
    10921 cgccgcaagg tctatgcacg ctgggccagg gtgcgcccca cgtcctttga ctgcaacctc
    10981 atcgtgatcg gcgccggcgc cggtggtctg gtctcggcct acatcgctgc ggtggtcaag
    11041 gccaaagtga cgctggtgga agcccacaag atgggcggcg actgcctgaa ctatggctgc
    11101 gtgccgtcca aggcgctgat ccgcagcgcc aagctggcgc agcagatgcg tcatggtgcc
    11161 agttatggcc tgagcaacag cacgccggtg ttcagtttca aggcggtgat gcagaggatt
    11221 caggcggtca tccgcgccat tgaaccgcat gacagcgtcg cgcgttacac cggcctgggt
    11281 gtggaggtgc tgcaaggcca tgcgcgcatc gtcaacccct ggagggtgga gatcaccctc
    11341 aacgacggcg gcacccagat cctgacaaca cgcagcatcg tgatcgcagc gggtgcccgg
    11401 cctctggtac cgcccttgcc ggggctggaa gaggctggct atgtgaccag cgacaccctg
    11461 tgggaggctt tttcgcaact cgacgaagtg ccccggcgcc tggtgatcct cggcggcgga
    11521 ccgataggct gtgaactggc gcaggccttt gcccggctgg gttcgcaggt cacgcaggtc
    11581 gagatggcgc cgcgcctgat gttgcgtgaa gatgtggagg tgtcggaact ggcgcgccag
    11641 gcgctggccg ccgatggtgt gtcggtgctg accggccaca aggccttgcg ctgcgaaacc
    11701 tcgaatggcg tgaaaaccct ggtcgtcgaa cacggcggca gcgaacagcg gctggacttt
    11761 gaccagctga tctgcgccgt cgggcgggtc gcacggctgg agggctacgg gctggaagaa
    11821 ctcggcatcc cggtgcagcg gacggtggac accaacgact acctgcagac catgtacccc
    11881 aacatctatg cggcgggcga cgtggccggg ccctaccagc tcacccatgt ggcggcgcac
    11941 caggcctggt acgccgcggt caacgcgctg tttggcgagt tcaggctgtt caaggccgat
    12001 tactcggtca ttccctggac caccttcatc gacccggagg tggcgcgtgt cggcctgaac
    12061 gaacaggaag cgcgggagaa aaacatcgcc tttgaagtga cccgttatgg catggacgac
    12121 ctggaccgag ccattgccga cggcgaggcg cgcggtttcg tcaaggtgtt gacggtgccg
    12181 ggcaaggaca cgattctggg cgtgaccatt gtcggcaccc atgccggcga cctgctggcc
    12241 gagtatgtgc tggccatgcg gcacggcctg gggttgaaca agatcctctc caccatccac
    12301 acctacccga cgctggccga ggccaacaaa tacgcggccg gcgaatggaa acgcgcgcac
    12361 cagccccacc gactgctcga atgggtgcgc cgctaccacg actggaagcg gggttgacat
    12421 gaagcgctgg ctggcggtgc ggctcgccgg actgctgctg cccctggccc aggcgcaggc
    12481 gcaggcggca ggaccgggcc tctccgcgtt ttcatcggcc actggagcgc tgccacctgc
    12541 gccctggcgc gtggtgggcg tgccgggcgg caagatcccg ctgaccactt atgccctggt
    12601 cgagctggac gggcgcaagg tgctgcgggt cgaggcagac aaatcgtatg gcaacctggt
    12661 gcatgacctg cctgcggcgg tgcccgaggc cgggctgcag ctgcgctggc gctggcggct
    12721 ggacgaggcg ctgcgcgggg gcgatctgcg ccgccgtgag ggcgacgaca gcccgctcaa
    12781 ggtgtgtgcg ctgtttgacc tgccgctggc caggctcggc ctgatcgagc gcaacctgct
    12841 gcgcctggcg cgcgcggcca gcggcgagaa gctgccgtcg gccgtcctgt gttacgtttg
    12901 ggacggcacg ctggcgccgg gcaccctgct gcccaatgcc tacagcgcgc gcgtgcgttt
    12961 catcgtgctg gacagcggcg agcagcgcct gggtcagtgg gtggctcatt cgcgcgatct
    13021 ggcggctgat ttccgccgcg cctttggcga tgaaagtgat acggtgccgc cactacaggc
    13081 cgtgctggtg ggcgccgatg ccgacaacac cggtggtcgc agcgtgggcc atgtcgggga
    13141 tgtcacactg tccccatgac tttcccttcg aatacacgac cgggcctgaa acacctggtg
    13201 ctgctgggcg ccggccatgc ccatgtgcat gtgctgcaca gcctggcgca aaaccggccg
    13261 gccgatctga acatcaccgt gattgccccg tacccgcgcc agctgtattc cggcatggtg
    13321 ccgggttttg tggccgggca ttacagcctg gaccaatgtg tcattccgct ggcgggcctg
    13381 ctggcgggtt gcggtgcccg ttacattgag ggcagcggcg tggctctcga tgccgaggcg
    13441 aaaaccgtga cgctggccag cggtgacacc gtggcctggg actggctgag catcaacacc
    13501 gggccggtga tggaccgcga aagcatcgag gcgcagatgc ccggcgcgcg cgaacacgcg
    13561 ctgtttgtgc ggcccatcga agccttcggc cagttgtggc cccaggtggc ggcgctgggt
    13621 cgcagccggc cgattcatct ggtggtggtg ggggcgggcg cggcggggct ggaactggcc
    13681 atggccgcag cccatacgct tcggagtgcc gactacccgc ccggtggcca cgtgagcctg
    13741 gtgaccggcg gcccgccgcc ggcctggaac tacccggccg gtgtgcagcg ccgggtggtg
    13801 caggccctgc ggcgcctgca gatcacggtg ctgcctgatg cctgtgtggg catgtcggcc
    13861 ggggaagtcc aactggccgg cggcgcgcgc ctggcctgcg acgccccctt gctggccgtc
    13921 ggcgcccagg cgccgggctg gctggccggc agcgggctgg cactggacga cgccgggttc
    13981 gtggccgtca accgctttca gcaaagcacc agccatgccc atgtgtttgc ggccggcgat
    14041 gtggccagcc gcatcgatgc gccgcatccg cgcagcggtg tttatgcggt acgtgccggg
    14101 ccgcccctgc tggccaacct gcgcgctgca tggcagggcc agccgctgct gccttacgcg
    14161 ccacccgggc gcacgctgaa cctgatttcc tgtggcgggc gttacgcgat agccgcctgg
    14221 ggccagttgc acgtggaagg gcgctgggtg tggcacctga aagaccgcat cgaccgcggc
    14281 tttgtggccc ggtacaccgc gccggcctga cctggaacat tcactgaatt gctctgtttt
    14341 tgatagctgc ttgcgcttgt gggataagga ctggaggccg aaaaggcatg aaaaaacccg
    14401 cgcaaggcgg gttgactcac aagcgcgtgg cgtttacttc ttggcgggct cggtcttggt
    14461 gtcggttttg ggcgtcaccg cggccttgac gtccttcgcg gtttccttgg ccgcgtcctt
    14521 ggtggcaatg gcagcacctt tgacggtgtc tttggtggcg accgcggcat ccttggcttt
    14581 ttcagtcatc ggcttgtcgg ctttcttgtc ggccttggcg ccggccttgt agctggtgcc
    14641 gttgacggtc aggccatcgg cggaaaactt ggcggcgctg tcggtgccca cacctttgac
    14701 gcgggtcaca aagtcattcc agtccttgaa cggcgctttc ttgcgctcgg agatgatcag
    14761 cgccgacttc accggaccga tgcccttgat ggcgtcaagt tcggccgagg tggcggtgtt
    14821 gacgtcaacc gcggcaaagg ccatggcgac atacatggtg gcgacaaagg ccaacagttt
    14881 cttgaacatc gggaagctcc tcaaaatgga tggttgaagc aggtgatccc tcccgtgcac
    14941 cgggatgtcc tgcgtttaac ggctgttgca gccgccaggt tgaccaaccc gtgtatttgc
    15001 gctggatcaa caatgcaggc gccgggaaag gcataagctg cagtatcgcc acaaagaagc
    15061 ctccaggcgg cgaaaaggag ctgaagatgc ccactcgttt gaaagacttt gcaaccgccg
    15121 tggtggctgt ggtcgagccc gagacctcgg cgttggtggc cgcccagctg atgcgcaagc
    15181 accatgtcgg cgcgcttgtc gttgtcgatg ctgcgcaaaa gagccgaccg gtcggcatcc
    15241 tgaccgacag ggatctggtg ctcgccttga tggccgaagg gctggatccc gaggtgttta
    15301 ccgccggaga catcatgtcc gtcgaactag tccaggcgac ccccgagatg gatgcgatgg
    15361 acgcggtggc actgatgcgc aggcaccggt tgcgacggct ggtcatcgtc gatgagtcgg
    15421 gccggctgac gggtgtcgtc acgatggagg acatcctggc cttgctgacg cgggagctgg
    15481 ccgatctggc cgccggggtg atcggtgcca gggaccggga attcgagcag cgcgcatgag
    15541 gagagcctca tgatggttga gaagtggctt gtttttcttg tggtcgttct ctggggcctt
    15601 gccgggcctg tgcttgcccg gaccaaaacc gatgccctgc gcggcgaact gctgtattcg
    15661 acccattgca tcggctgtca caacgcccag cttcactggc gcgacaaaaa ggcggccaac
    15721 aactgggcca gcctcaaggc cgaagtcgaa cgctggcaga aaacctcggg gctgggctgg
    15781 cgggaggagg acgtgaccga cgtggcgcgt tacctcaaca ccctgtatta ccgttttccc
    15841 gagctgcggg gcgatgctgc ttcgacggca gacgccgccg caccggcgcc cgcccgctga
    15901 cgggcgctgg tcgcgggccg gtcaggcttt tgcggcttcg ccttgggtca aggcgcgcat
    15961 gtaggccgcc acgccggtct gcacatcggc aaacacatgg tcgcagccgg ccgctcgcag
    16021 ggccgtcaga tcggcttggg tgtagctctg gtacttgccg accagcgcga cggggaaatc
    16081 gatgtagtcg atcaggctgc cgtgcgcggc ttcttccagc gtcagcggcg catcgccctg
    16141 ctgctggcgc agggtgttga cgacggccag cgccacatcg ttgaagggct gggcacggcc
    16201 agtgccgagg ttgaaaatgc cggactttcc ggggttgtcg aaaaaccaga gattgacggc
    16261 cacgatgtcg tcgatgaaca cgaagtcgcg catctgcccg ccacggccgt aaccgccgta
    16321 ctcgccgaac agcttgacct tgccttcggc gcggaactgg ttgaactggt gaaaggcgac
    16381 actggccatg cgccccttgt gctgctcacg cgggccgtac acattgaaat agcgaaagcc
    16441 ggcgacctgg gtggccgtgc cggaaaaacc ggttcccagt tcgcgccgca gccgctggtc
    16501 gaacagcagc ttggagtagc cgtagacatt gagcggcttc tcgaactcgg gtgactcacg
    16561 gaaggtgtcc gacccaccgt aggtcgcggc cgaggaggcg tagagcagcc gggtgcgctg
    16621 ttcctggcag gcggcaaaca agtcgcagga cagcgcgtag ttgttgtcca tcatgtactt
    16681 gccgtcgctc tccatggtgt cgctgcaggc accctcgtgg aaaaccgcct cgacgttgcc
    16741 aaaggcggat tcggcaaaca gttcgtagaa atcgtcggcg tcgatgtagt cggcgatctg
    16801 caggtcggcc aggttgcgga acttgtcgcc gtcggtcagg tcatcgacgg caatgatgtt
    16861 gtcgatgccg cgggcattga gtcccttgat gaggttggag ccgataaaac ctgcggcacc
    16921 cgtgacgacg attttcatgt ttgttccttg tgttcagggc gcggcaaaca gctcgccgta
    16981 gctgaccgtc gccgtgccga atttgccgac cacgatgccg ccggcgcggt tggccaccgg
    17041 gacggcgtcg cgcaggctca ggccggcggc agccatcgcg gccagggtgc cgatcacggt
    17101 gtcgccggcc ccggtcacgt cgaaaacctc gcgcgccagt gccggcacat gcagctggcc
    17161 ttgggcgtcg aacagcgtca tgccttcttc actgcgcgtc agcagcaggg cctgcagctt
    17221 gaggacctcg cgcaggttct gcgccttgcg ctgcaggtcg gcttcatcct gccaggggcc
    17281 gatgacctgc tcgagttcgg cgcggttcgg ggtgatggcc gtggcgttct ggtaacgcga
    17341 atagtccgag cccttggggt cgaccagcac caccttgccg gcggcgcggg cctgcgcaat
    17401 catcaggccg atgtgcgcca gaccaccttt gccgtagtcg gaaaacagca ccgtgtcatg
    17461 cgccggcagc agtttttcga actcggccga ctgcgaggcc agcacctcgt tttcgggcgt
    17521 gttttcaaaa tccaggcgca acagctgctg ctggcggccg atcacgcgca gcttgaccgt
    17581 ggtcttgagc tgcgggtcgc ggccgaagca gggcgtgatg cccgagccgg ccaccagggc
    17641 ctcgagctgg tggctggcct cgtcgtcgcc gaccacgctc agcagcgagg cgcgcgcgcc
    17701 cacagtcacg atgttatagg ccacgttggc cgcgccgccc atgcgttctt cctggcgcgt
    17761 gacacgcacc accggcacag gcgcttccgg cgagatgcgg tctaccgccc cgtaccagta
    17821 gcggtccagc atggcgtcgc cgaccaccag cacgcgggcc ttggctagct gttcgcggga
    17881 cagagttaaa gatgtggtca tgttgagaga atcttattca agcggacgcc gcggaaccgg
    17941 ctttgccggg ccgctggtgt ccgccccctg caagggggaa ggcaagcgaa gcgcagccac
    18001 gggggtgttc ataactcttc gatgcgccgg gcagggtagc tgtcccaggc ctggcagccg
    18061 gggcagtgcc agaagtgctg cgtcgcttcg aaaccgcagg cggcgcagcg gtaacgcatc
    18121 aggggcttgg tggcctggtc gagcgcgcgc tgcaccatgc tgtgttcgtg ctcgctgccc
    18181 agtttttcac cggcaatcca tttggccgct gccaccagcg aggcttctcg ctcgaggtgc
    18241 cggccgtacc attggcgcgg atcggcggac tctgtatcga gtttgacgat ggcctcgatc
    18301 acgtcaatcg acggcagctg ggcatagttg gccttcagca ggtccagcgc ggccgcttgc
    18361 tggccgcttt gctgtgcaat gttgacgagc aggccggctg ccagcggcaa ggcctggggg
    18421 gcggttcgct ccagctggcg cagggttgca aacgcatccg catgttgacc cagctgggtc
    18481 tggagcttgg ccaggtcgat cagcgggcgc ggtgaggccg gcgccatgac ggccgcctgt
    18541 tgcaggctgt cgagcgcctg gccggtatcg ccggaagctg ccgccgacac cgcctgttcg
    18601 cacaggtagt gggcctgccg ggcgctgaag ctgccgtggc tggcgctgtc gagcttggcg
    18661 gcgatatcgg tggcgtgtgt ccagtcgcgc gagcgttcat aaatcgacag cagagccagc
    18721 ctggcttctt cttcataagt cgtgccttcg agctttcgca gcgccacctc cgcgcggtcc
    18781 agcaggccgg ccttgaggaa atccagtgcc agcgcgtgct gcgcacggtc gcgctcggtc
    18841 tgggtcaggt cgccgcgcga catcaggtgc tcgtgtacac gcacggcccg ctcgtattcg
    18901 ccacggcggc gaaacaggtt gcccagtgcg aagtgcagct cggaggtgtc ggggtcgttc
    18961 tggacggctt caataaaggc gtcaatcgcc tggtcctgct gttcgttgag caggaaattg
    19021 agaccgcgga agtaagcttt aggcgcctgc cggttttcga tgcgcatctg cctcagatcg
    19081 aggcgggagg ccagccagcc cagcgtgaag gcaatcggaa acccgagcag aacccaggtg
    19141 aaatcaaagt ccatggtgca tggtggaggg gttctcgccg agttggctct gctggctggc
    19201 gcgcacgttt ttgcgttttt tccaccagcg cggcatcatc atcaggatgc cggtggccag
    19261 cccgcaggca aacgtgacca gcacgaccag caccaggggc gcctgccaca gcgtaccgaa
    19321 aaagaaatac acggaaacgg cttgctggtt gttcagcgcg aacgcaaaaa gcgtaaaaaa
    19381 aatggctgcc ttgagcagcc acatgaggta tttcactaca ggtccccttg tggtccggca
    19441 attgtagcca gca
//
LOCUS       DAIDFG010000016        18997 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_16_length_19035_cov_19.208904, whole genome shotgun sequence.
ACCESSION   DAIDFG010000016 DAIDFG010000000
VERSION     DAIDFG010000016.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 18997)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18997)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18997
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16_length_19035_cov_19.208904"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3304 a         5893 c         6244 g         3556 t
ORIGIN      
        1 acgtgatttc cggtgcgatt ccgcccaacg agcggattgt cacgatcgag gacgctgccg
       61 agctgcagct gcagcagccg catgtggtgc ggctggagac ccggccgccg aacatcgagg
      121 gcaagggcga ggtgacccag cgcgccctgg tccggaacag ccttcgcatg cgtccggacc
      181 ggatcgttgt tggcgaggtg cggggcgccg aggtgcttga catgctgcag gcaatgaata
      241 ccggtcacga aggttcgatg acgaccgtcc atgcgaatac gccgcgcgac gcgctgaccc
      301 ggctggagca catgcttggc atgacaggca tgcaggcgaa cccgcgcacc atgcgccagc
      361 agatcagttc ggcgctgtcg gtcgtggttc agatttcacg cctttccgat ggccggcgca
      421 aggtcaccag cctgcaggag atcacaggca tggaaggcga catcatcacc atgcaggaga
      481 tcttcctttt cgatcagacc ggcgtcacgc aggacggatc cgtgcagggg cgctttcgcg
      541 ccaccggcat ccggccgaag tttgcgcagc ggctgctgac ccgtggaatc gggctggccg
      601 aggatctctt cgatccggcc cgttaccagg attgatgcca tggatccgct ctactccatt
      661 tttgtggtgc ttgccttcct cgcggtggtg ctgttcctgg aaggactgta tctgacctgg
      721 aacgcttacc gcggtcccga ggcgcgccgt atccagcagc gcctgcagtc gctttccgcc
      781 acgggcaacg acagtgttgc ggcgccgctt gtcaggaagc ggctgctgag cgatgtttcc
      841 actatcgagc atctcctgtt gaagctgccg cgcattcatg tattcgaccg gtttctggtg
      901 cagtccggaa tgaacctgag tgtggcggag gtactcggca tcgcggccgg acttgcgttt
      961 gccgtcggca tggtgcttgc gctcgccgga ctgcctctgt tgttgagcgc gggcgctgcc
     1021 atgcttccga tcgttctctg gattggctat atccagcgcc gccgcatgca acgcatgcgc
     1081 ctcattgagc agcagttgcc cgatgcgctc gacctgatgg cccgcgcgat gcaggccggc
     1141 cacgccttct caagcgcgct gcacctggtg ggtaccgaag gaccgcagcc gattgcgcag
     1201 gagttccata cggtctttga tgaaatcaac ttcgggattc cttcagagaa agcgctgggc
     1261 aacctcgcct cgcgcgtcgc cagtggagat ctgcgcttct tcgtgatcgc agtcgtgatc
     1321 cagcgtgaga ccggcggcaa tctcgccgag atcctcacgt ccatcgcggc gctgatccgt
     1381 gagcggcaaa agctggtcgg aaccatccgg gttctctcgg ccgaaggacg tatttcggca
     1441 tggatcctca cgctcatgcc gttcgtgctg gcggccgcga tcagcctggt caatcgtgaa
     1501 ttcatctcga agctgtggac cgatccgctg ggtcttcgca tggtcggcat cgcgctggcc
     1561 ctcatgctgg tgggcgtctg gtggatgtgg cggctggtga agatccgcat ctgacggagg
     1621 aacataaaaa tgacgaattt ccaacctgtc tttttcgccg tggtgtttgt gattgtcgcg
     1681 ttgatctgct acctggtcct gcgccagttg ctgccaacgg cactcgacaa acgtgtcgag
     1741 gcgctcggtg gtgcccatcc ggattcatcg ccgcggccgg gcgttgcggc atcgctgtcg
     1801 ggggctgttg cacgctggct gacgccattt gcgaggcctt cgctgcccaa gggggcctgg
     1861 gaaaaatctc cgctgcgcct gcgcttttac aacgcgggat tccgcacgac gacagcaccc
     1921 attgcctact tcggcgccaa gactgtcctg accttcgcac ttccggccgt gcagctgttt
     1981 tacgtctgga ccttcccggt cgaactccag gcgaccccgc tgctcgccct cctggttttt
     2041 ctggcggcct tggggtacta cctgccgaat gtcgtgctgg cgcacatcat ctcgcgccgc
     2101 caacgcgaac tgttcgaggc atttccggac gcgctcgacc tgatgcgtgt ctgcgtacag
     2161 gcgggcttgg gcatggacgc cgcgatcgag cgggtgggac gtgaaatgga gctggagagt
     2221 tctgcgcttt cggaggagtt tcacctggtc agccttgagc ttcgtgcggg cgcctcgcgt
     2281 gccgatgcct tgcgcagtct tgcgttgcgc gtatcgctcg aggacatcga tgcgatggtc
     2341 gcgacgcttg tgcaggccga tcgcttcggt accagtgtcg ccgagtcgct tcgggtgcat
     2401 tcggagacgc tcagaaccaa aaggcggttg atcgccgagg agtctgccgc caagctcccg
     2461 gtcaagttgc tggtgccgct gattttttgc gtatttcctg cgttgctcac ggttctgctc
     2521 gggccggcgg tgatcagcgt gtaccggatc ctgcttcccc gacttaccgg ccaataacca
     2581 aggaaaacac catgaactcc catgctcacg cgcgttccca gccgccccgg aaggcccatg
     2641 cctgcgtcaa ggcggatcga caggaggtca ccatgcccag gttcaggacc atcgtgtcat
     2701 tgatcgtcgg cgccggtgcc gtcatgggtt gcgcgtcgcc cggacaggag caattcgccg
     2761 ggccctatgt tcggcccatg cagactgtgc gccatggcgc cgattcacag gccctgtacc
     2821 agacgggccg ctattttcag ggacagggcc gttacgagca ggcgatcgct gcgtacagtc
     2881 aggtgctcaa ggcgaacccg gaccatgtgg acgcgcacaa cgcgcttggc gtgacctatt
     2941 cgcagcaggg gcgaccggag cttgccgagc agcagctcag ggctgcgctt gagatcgcac
     3001 ccgacacggc gcgcgtacac aacaatctcg gctatcacct gatgggcagt gggcggctgg
     3061 aagaggcgct cgcctcgttt gagcgtgccc tcgcgctggc gccgaaggat gccctgatca
     3121 ccgcgaacct ggcggtggcg cgggagaggc tgggcctgcg caccgatgtg cagcggccca
     3181 cggttcccca ggcagccgcg caaccggtcg cggtggtcct cgcgccagcg caggcgcttg
     3241 cgtccgcccc tgtacccgac gtgacgatgc ggcttgatcg catctccgac ggcatgtggg
     3301 cgttgcgcag ttccacggca gcgcagcctg caacggcaac tgcagccggc acccggactg
     3361 ctgcggccgc gcaagaccgt atcgaagtct acccgcgcag cgtcgcggtt gtgccgtccc
     3421 cggctgccac ggcaaacagg attgcggccg aggcccgcat cgagattgcc aacggaaatg
     3481 gtgcgaccgg gcttgcgaag cgggtctgga acctgcttgc accgcacggg atggagcgtc
     3541 cgcggctcac caacgacaaa ccctacggcg tggtcacttc gcgcatcgag tacgtcgctg
     3601 gcgccgaaca ggtggcgcgc gatgtcaacg cgggtctgcc tgcgcagttg ccgctggcgc
     3661 gcgtgcctgt tctggagcgc aacgccagcg tacgcgtgtt gcttggcaag gattttccgg
     3721 gcattgcagc ggcggttgtg gcggacgcgc aggtgccagt caagttgacg gctgcaagtc
     3781 catcaggggg gccgctcaat cagtgacttt ttcaaccggc tttttgacct ttcatttttt
     3841 taaccaggag cttctcatga acgaccaacc tatcatcgac ccgaaaaatc tccctatctg
     3901 gacaacggcc ggtttcacga tcgcgctgct tgcgctcgtc ctcgccctca tcgggctgta
     3961 ccgcaccaat gcggtcctcg tcgccacgca aatgcagatc atttcactca acaagaaggt
     4021 cgcagagcag ggcaagcgcc tgccacccgc gccgcaggcg gcagctgccg cggccccgca
     4081 ggcgtctgcc aatccggcga tcaaatagtc cctgctgaac gcgggcatgg gagccgtgcc
     4141 cgctgtccgg catactgtcg gataacgcag ttttcccttc ggtgaattgc tttacctgat
     4201 tggcgggcat cgatgggctt tggggcattg tcaccgttcc tgaattcgct gagaggtcgc
     4261 gtcctcctgc tggtctgcgt cccgtttgcc ttcttgctgg gtctcctggg gtatcaggcc
     4321 gtcagcgaca aggattttca tctccaggaa atgcgccagc gtgtccgcga tgcggcggct
     4381 gtgatagtgc tccagaaaac cggcgtcatc gagcataccc ggttgctcgc caccagcctc
     4441 gcacaagcct cgcagaggcc ggtgcttacc gtctcgtcca cctgtctgcg caagctgtcc
     4501 gacagcatgc gggaagccag gcacatcgcc aagatgctgc tcatcctgcc tgtcggcgag
     4561 acggtctgca gtacgctgaa gaatgacccg gaggtcgatt tcagtgctca tcccgagttt
     4621 catcgggcgc tcctgacccc gaatgtcgtt gtcggagaag cacgccccgc cggcatgagt
     4681 ggaagactgg gcttgccgtt ttacatggcg atacgggaca acaacgacaa ggtcctgggt
     4741 gtggtgatgg tggttttcga tctcgcctgg ctcaacagcg aaatcggcag tgaccgtttt
     4801 cccccggggt cccggatcgg cctgatcgcc gccgatggaa ccgtgctctc ccattctcct
     4861 gatcccgagg gatgggccgg tcggcgcata acggaaacgt ccttcttcaa gtcactgatt
     4921 gcgccgggca aggaacaggt cgccgaggac actggattcg acggcgagaa gcgggttgtt
     4981 gccgtgacgc gattcgctga aaccaccggc ggaccgatac tgctttggat cggtattccc
     5041 aggcaatatg ccgatgggct cgtggagcag caattttcct gggcgctggg gctggccatc
     5101 gccttgctga cgggcgtttt cggcgcgatt tattgggggg gcaaccggct tctggtcagg
     5161 cctgtcgagg ccctggcgaa agcggcccgc cgtcttgggc agggggacgt gagcgcccgc
     5221 agtggcctga accactcgag cgatgagttc ggccagctgg cagctgcttt cgacgacatg
     5281 gcgcagtcgc tgtcgtccct gcgccagacc ctgcgcggaa gccgggcgct gcgcgtgctg
     5341 gccgctggtg cccgggagcg ggcgggcggg aaggacgaca tcgcgctggc cgaggacacc
     5401 tgtcgtgcgc tggtggacgg tggcggctac cggctggcct gggtgtcgta ttcacagggc
     5461 gcgggcgatc gccaggagcc tgttgcccaa tgggggctgg acgccccatc cgcgcagatc
     5521 ctgcaagtgc tgtggagcag ggcattgcgc cagacctacg gcagcaacgt tgcctccagt
     5581 gatgatccag tcgttatccg tgacattttt tccggaacga cagcgcaagc gcttcgcgat
     5641 cttgccgggc aacattcctt tgcttcaggc gcttcctttc cgttgattgt cgatggcgcc
     5701 ctgatcggtt ccgtcagcat ctgtgcgcag gaggcagacg ccttcgacgt ggaggaaatt
     5761 gttcttctca aggaggccgt caaacacctt gcactggcga ttgctgtgca acgagccaat
     5821 gcaacgcgca tttggctgga atccagcctg gtgggcgcga aagatctgtt tcgcgccgcg
     5881 tccaatgcca ccgtggacgt tttgtttttc ttccgcagcc tcaaggatga gacaggccag
     5941 gtggttgacc tggagtgcac ccacaccaac gggcgcgcaa aggccatgtt tggcgaggtt
     6001 gcgaacacat tcatcgggaa aagggcagct gaaatcctgc cctttgacaa gggcagcggt
     6061 gtcctggaaa aattcctgcg cgccgccatg acagggtcgc ctttagaggg ggagtccgag
     6121 accaatgccc ttcccggtgg accccggtgg atgcggtttc aggcggtgcc tctgcgcgac
     6181 ggcctggcca tttcggtgca ggacatttca gcgcgcaagc gtgacgagca ggcattgcgc
     6241 gatcgggctc tccagcagga gatcatcgcg tcgctgggcc gctctgcgct ctccgaggct
     6301 gaccctgacc acctgtttgc gggggctgcc gctgcgatgg cccagggcct cggcgtggcg
     6361 tacagcaagg tgatgcaact cgtccccagc gaccgatctt ttgtcctgaa ggcgggcgtg
     6421 ggctggggtc ccgactgggt gggtcggcgc atctccggcc tggatgatgt caatgtccgg
     6481 cttgccgggg ggatttcgcc gcgccaatcg tgggtcatcg acagccacgc cggcgacgcg
     6541 caggatccgg cttctgaaat tcttcgtgca cacggcatcg tcagcggtct ggatgtggcc
     6601 atcgtcggca gcgagggcct gtgcggcgta ctcggtgtgt actcgcgcga accgcgccag
     6661 ttcagtgcgg acaatctcga ttttctgcag agcattgcca atatccttgc cacggccatg
     6721 gaccgaaacc gtggggaaga acaactggcg cacctcgccc aatacgatcc cctgaccgac
     6781 ctgcccaaca gggcgctcct caaggaccgc ctttcggttg cgttggcaga ggcgcagcgt
     6841 tcggggaaac ggcttgtcgt gatgtacatg gatctggacc ggttcaagaa cgtcaacgat
     6901 tcgttcggac acgattttgg ggacaagttg cttcaggaag tggctcaccg attgagcgcc
     6961 gctgtacggg cgtccgacac ggtgagccgg cagggcgggg acgagttcct ggtagtgctg
     7021 ccggaagtcg acagcgagag ggatgcagcc cgggtcgccg agaagctgat cgcgtcgatc
     7081 gccgagccgg tgaccgtcgg gggggtggaa ctgtccgtga ccggaagcat cggtatcgcg
     7141 tgttacccgg acaacggcag cgacatggat accttgctgc gcaacgccga cgctgccatg
     7201 tatgccgcca aggaaatggg gcgggaccgt taccagttct attcgcagga gatgaatgcc
     7261 catacccgcc agcgtctcat gctggaaacc gacctgagac gggccatcga gcgcaacgag
     7321 ctttttctcc agttccagcc gcaggtggca ctgggttcgc tggcggtcgt cggcgtggaa
     7381 gtacttgtgc gctggcaaca tccggtccac ggcgagatgc tgcccgacga cttcattccc
     7441 attgcggaag agatcggcct cattgtttca attggacgct gggtgctgga ggccgcgtgc
     7501 ctgcagcagg cgcaatgggt gcgggagggc attgcatgcg ggaccctggc ggtcaatgtg
     7561 tccgcgccgc agtttcggca acccactttt gttgccacgg tcatgcagat ccttgcgcag
     7621 accgggcttg agccgggcca tctcgagctg gaagtgaccg aaagcatcgt catgcaggga
     7681 acagacgtcg tgcgcgagaa gctgatgagt ctggccagat taggcgtgaa attcgccatt
     7741 gatgacttcg gtaccggtta ttcgaacctc agctacctca aacgctttcc tctccaccgt
     7801 ctcaagatcg accagtcatt catcagcgcg ttgccggggg acaaggagag cagggccatc
     7861 gtccaggcgg tggtcagcat gggccacagc ctcgggctga aagtcatcgc ggagggcgtc
     7921 gagaccgagg cgcaggcgga gttcctgcag gcgctgcagt gcgacgacgc gcagggcttt
     7981 ctgtacgccc gccccctgct gccggccgat tgcgccgcgt ttttgcgcaa cgatggctgg
     8041 cctgccgatg gcccggtcca gcaggggatg tgacggcatc gaccaggaca ggacaatacg
     8101 gctaccgttt ctgcagcttc tggcgcacca gctggatatt gttgcgaagc gcataaagct
     8161 cgtcggcgta ggacagcggc acgctgattt tttcggcgcg gcgttcgagc tggttcagct
     8221 cttcgagcag ttccggggtg cccccggttt cttccgcccg catctcgata tggcgcagct
     8281 ggccgtacca gcggaagata cgtgagcgca cgcgaaactc gtaaagcggt ggcacgatgc
     8341 gtgacagcgg cagcagcacc gcaatgatga tgcccatcac cagccacatg cgctcgacca
     8401 ggttggccac ccagaacggc aggtagcgct gcaacagcgg cgcgccggcc tggatgcttc
     8461 gctcggcttc cttggccacc ggccgttcgt tgttcttgac attggggtat tcgcgggcgc
     8521 gcttgaacca gccggcgctg ccgtggatct cgttgccggc cagcgcaaac agctgcagca
     8581 gcgccggatg ggtgctggta cgcgtgagca aggtggtggt gggcgcgacc aggcgcacgt
     8641 cctgcggcgg aatgttgccg gctagatcca ccaccccgcg cggcagcacg gccgggctca
     8701 ggaaggcgaa gcgccgggag taggcttcgc tctgggcaaa gtccatcagc ctgatgcccg
     8761 gtgtctgcag cagcatctgc accatcagcg attcaggcgc cgaagcaaac acaatcgcgt
     8821 ccagttcgcc ctgcaggaag gcaacggtgg cgggggtctg ttcgagttgg gaaatcttca
     8881 gcgcgccggg ctcgatgcgg ttgctttcca gcagcttgtt catcaggttg ggcacaccgc
     8941 tgccgggcgt gccgacgttg acgcgcaggc cctgcagttg cgtcagcgag gccagcaccg
     9001 cgccggcgtc cgcgccctgg ccgctgttgc cggggggtgc cgccgcgact ttgcgtgcgg
     9061 cctcttcgcg atagaacagc cacaccggct caaggaacag gctgcccagc gattcgagat
     9121 cctcgtcggt gtccacatcg gtggcgctgg tgccgccctg cacaaaaccg agatcggcct
     9181 tgccttcgcg cagcaactgc aagttggccg cggagccttc ggagggcagc agctccacgg
     9241 tgatgccgtc tttggcgagg atgcgggcgt aacgtttgcc gaattcctca tacgcacttt
     9301 gcgcgggacc ggtggccagc gtgacacgtt tgggcgggtt cgggtccagc caccaatagg
     9361 ccagcaccag cagcgtggcg gccagcacca cgaaggggcc ggcagagacg gccagatcgc
     9421 gcagcgacag cagggtgtag cgtatggttt ttggcatgcc ggcaccttaa catggacccg
     9481 cccagactac ggcgggcatg tccttttcag cataaaaaca ggctgcagcc caatttccat
     9541 ctgcgccgcc agctagatta ttgatagcgt caggagccgc cgtgcaggaa caccagcacc
     9601 gcattgagca ggtaggtggc cagcaggccc acgctgaccc agctggcaaa acgcaatacg
     9661 cgcccctggg gccgcatcac cagcccgatc atcaccagcc cgctcatgac cacggcggcg
     9721 accgccgtgc ccgcatgcac gggtgcggcg tcggcaagca gcgggccgcg ggtgtaaaag
     9781 aggtcgtcga tcgccagaat ggtcacgttg aacaggttgc tgccgagcag gttgccgatg
     9841 gccatgtcga gcgcacgcag gcgcagggcc gacagcgtga ccgcgatttc gggcagcgtg
     9901 gtcaccagcg ccatgaacag cgtgccgaca aagctgcggc tccagcccat ggcctgggcc
     9961 agctggtcgg cgatggcggg cagccagctg ccggccgcca gcaccaccag cgctgcgagt
    10021 ccgaagcgga tccactgccg cctcagtggc ggcgttggtc cggaggtcgc atcgcgatcc
    10081 ggcttctgcc gcaacgactg ctcgtgtccg aagacgctgc gcagcgccag caggtaaagc
    10141 gccagcagca ccgggctgta cagccccaga tgcagcatgg ccggtgtggc ggcaggcagc
    10201 agcaggctca tggcgacaaa gcccagcatg accacgccaa aggcggcaga cagcaggtgc
    10261 gccgcgctgg catggcggta gatcggctgg cgcggctgca aggcatcgat caccaccagg
    10321 aacacgaggt tgaacacgca ggcgcccagc gcattgccta cggccaggtt gggcgcattg
    10381 atgaccgtga cggcgctgat gcccgaggcc agctcgggca gcgaggtcac ggtggccagc
    10441 agcgccaggc ccatccagcc gcccgtgagt ccggtggctt tggcgatctg gtcggcactc
    10501 tggcacaaca cataacccgc gccggcgatc agcaccgcac acagcaggaa ctgcgggacg
    10561 agtacgagca ggctcatgcg gtccgcttct ggcgcaggcg aacgacgaac ttgcgcagtt
    10621 cttccgccca caacacgctg ctggccagca gcagcaaggg cagcagggag gcgggcgcca
    10681 gcggcaccgt gtagaacagg gtgttcatga acggggcata cagcaccagc acctgcagcg
    10741 cgatgctcag ggccaggccg ccgagcagcc aggggttttt cagcaggccc agccggaatg
    10801 ccgagcgggt ggccgactgg cagttgagca cgttgaacca ctggcacatc gccagcacgg
    10861 tgaaggtttc ggtacgcacc agctcaatcg gcaagccctg ccccagccgc cagacgaacc
    10921 agccgaaggt gaccagcacg gcggtggccg acatcagcag cacccggccc agcatggcgc
    10981 ggtccaccag cctgtcgttg cgcggtaccg gtgtgcgctt catctcgtcg ccgtcggggg
    11041 ggtccatcac caggttcacc gtcagtgtgc cctcggtcac gatgttgatc cacaggatct
    11101 gcaccgccgc cagcggcagc gggtagtcgg ccagcagggc cagcagcagc accacgacct
    11161 cgtcgatgga ggttgcgaac aggtagagga tgactttttt caggttgccg taaaccacgc
    11221 gcccctgctc gaccgcgccg acgatggtgg cgaagttgtc gtcggtgatg atgatcttgg
    11281 ccgcgctttt ggccacctcg gtgccggtga tgcccatcgc cacaccgaca tcggcgcgcg
    11341 ccagcgccgg tgcgtcgttg acaccgtcgc cggtcatcgc caccacctcg ccgtgtgcct
    11401 gcagggcttc gacgatgcgc agcttttgcg ccggatgcac acgcgcaaag acggaaatga
    11461 gatcgagccc gtcgcgcagg tccgcctcgt tcatgcgttc gagctcggcg ccgtccaccg
    11521 cacggtcacc gtcacgcgcg atgccgagct cacgggcaat cgccaggccg gtgagtttgt
    11581 ggtcgccggt caccatgacg ggccggatgc cggccgcgcg gcattcggcg acagcggcct
    11641 tgacctcctc gcgcggcggg tcgatctgcc cggccaggcc gagcaggtgg gcgcgcccgg
    11701 ccagcgcgtc gaaaccggcc tcggggtcca gcacatcgtc ttccaccacg gcaaaggcca
    11761 gcacccgcag cgcgcgtgcg gccatggcgt ctgcggccgc gcgggccgcg tggatcacgg
    11821 ccgttccgtc ggccgcgcac aggtgcagca gcacttcggg cgcgcccttg atgaacacgc
    11881 ggcgcggtgc gccggcgaag cgatggcgcg tggccatcat gcgggtgtcg gcatcgaagg
    11941 ccagttcggc ctcgcgcggc gcctcgcggg caagttgttc cagattgatg cccgccttgg
    12001 cagccagcac cttcagcgct ccttctgtcg ggtcgcccag caccgaccac tgcgtgcgcg
    12061 cctcctcggg tggcagcagc tgcgcgtcgt tgcacagcgc ggcagcctgc agcagcggca
    12121 gcagggccgc atcatcggaa ctcaaccggc cctcgggcgc atagccggtg ccgctgacgg
    12181 acacctccag cccgccgggc agccacaagg cgctgaccgt catttcgttg cgtgtcagtg
    12241 tgccggtctt gtcggtgcag atcacggtgg tcgaacccag ggtctcgacg gccgacaggc
    12301 ggcggatgat ggcgccgcgc cccgccatgc gctgcatgcc gacggccagc gcaatggtca
    12361 tggccaccgg caggccttcg ggcaccatgg acaccatctg gctgatggcc accatcagca
    12421 catcggccag cggcagatcc cggaacatcc cgagcgcgac caccgcgaca aaaagcacca
    12481 gcgcggcgac gaccagcgcg cggccaaatt gcgcgatgcg ctgctccagc ggtgtctggg
    12541 gatcggctgc ggtttcggtc agcccggcaa tgcggccgac ttcggtgtgg ctgccgatgg
    12601 ccacgaccac cgcgcgcgcg cggccggcgg tggcataggt gccggagaac agcatgttgc
    12661 ggcggtccgc cagcccggtg gactcggcca gtggcgccac cgccttgctg accggaaccg
    12721 actcgccggt cagtgcggcc tcggcgacct gcaactgggc ttcctcgatc agcctggcgt
    12781 cggcaccaat cgcgtcgccg gcggccagca gcagcagatc gcccggcacc agctcacgcg
    12841 cctcgaccaa tgcctcctgc ccgtcgcgta acacccgcac ccgcagcgcc gccagccggc
    12901 gcaacgaggc catcgagcgt tccgcgcggc cttcctggaa ggtgccgacc acggcattga
    12961 ccagcaccac ggccaggatg accacggcgt cgccgtaatg cgccatcgca accgccagga
    13021 ccgcggcggc aaacagaatg tagatcagcg ggctcctgaa ctggcgcgca aacacggcca
    13081 gcatcgagcg gcgctgcgct tcgggcaggg tattggcgcc ttcgcgcgcg cgtcgttgct
    13141 ccacgtctgc ggcgctgagc ccgacggcgg ggtcgcaagc cagctgggcc aggacctcgc
    13201 cggccggcaa ggcatgccac agtggagagg cgggcgcagg cccgctgtgt gaggagaggg
    13261 tcttggcggt catgggaagg gcaggttgtt gccgtcaggg tagcgcaaaa atcggccacc
    13321 ccggcccgca agccgcagcc tcggcgaacg ggatggcctg ccgcttgcgt tacatcaagc
    13381 cccgtgccat tgcggtgatc acgccgccga tcagcggccg aggctgcacc gcaggacgta
    13441 tggctgtttg catggcgaca tcatgtgggg gttcgatgga tcgaacggtc tcggtctcgc
    13501 ctgcacgaaa acgcaaaagc cgggcaggaa tgtgcacaag aatcccgcag ggtggcagcg
    13561 gagggacgta cccacatgcc tcaactgttt gatgcatatc aatcagccat cgcgggaccc
    13621 cggtagcatg aacgaatcga aacctgtacc tccatgaagc tttctctcaa cacccgtacc
    13681 aaacgccgca ccgcattcct ggtgttgctg gggtggctgt ttgcgctggt ttcaggggtt
    13741 gccaatgcgt gcttgctgga agcgcgcgtc acgcacaccc acgctgcacc cttcgaatta
    13801 tccgaaaccg tgcaggcccc ccttgtctcc gcaggtcatg cgggcgttgt ggctgaccac
    13861 gacgactcgc atgttgccaa ggccccgtgc ctgaaggcct gcgacgatgg cgcgcattcc
    13921 ttgcccaagc gggacttgac ggcgacccag gcagatcctg gcccggcgcc tctcatgtca
    13981 attctctgga cagcggcaac gcctgttgct ccgacgactt ggcgaatgga cgacgtccag
    14041 cccgccttac ccgagcggcc catccgggtc cgctattcac ggctggcact ttagccgcct
    14101 tctgacggct ggcccggaac ctcgcgctcc gcatacctgc cgtccacttc cgttccaggg
    14161 cgccatctcc aggtgattgg gcttggggac gcgcgttgcg cctacgcctg cgtttacttt
    14221 acgccagtac gccgcatttt cagcacagat tatttcctga ggagatcgac atgtttgaca
    14281 cacgagactg cccattgagt cggcgtagct tgttcgctgg tgcgctgacc tgcgctggcc
    14341 ttgtgctggc gacagtgcct tggggtgtga tggctcaggc aagtcctgaa gggatggcca
    14401 cacggtcgac gagcgatcag ggcgtgacga tcaaggtgac cccgaagtcg ttcggaagcc
    14461 cggacagccg gtgggagttc atggttgtgc tggatacgca cagtgctgac ctgagcgatg
    14521 acctgacccg cagcgccacc ctggcgaccg acgagggacg cacattcaag ccttcgagct
    14581 ggcagggcgc accagccggc ggccatcacc gcgaaggggt gttgaccttc gatgttccgg
    14641 cgccacgacc gggcgtgatt gaactcagga ttgtgcgacc gggtgaatcc gccccccgta
    14701 tttttcgctg gctactttga aggagagtca tcatggaccc gtttgacaat gatcgacagg
    14761 cgccttcgtt ctggcgtcgc cctgccggca tggcggtcgc aacggccgcc ctgattgccg
    14821 gcttctacct gctgcgtgag cactggggcc atgtcctggg ctactggccg tacctgcttt
    14881 tgctggcctg cccgctgatg cacctgatgc atggacacgg ccatggcggc cataagcatc
    14941 ggcgagacga gccggagcgc acgaaccaag gtgcgggtta aggggatggg actgaacacg
    15001 catcaccatg gcccgcggag caaccgtgca tgagtgcacc gttccagctt catcgggaag
    15061 cgctggtcgg gattgccgca gattccgcag ccgtcttcgc gtggctggac gatcagacgg
    15121 ctgtccgcgc acatggagaa acggtctctc atgatggcgg gcgcaacgat gaaaactgag
    15181 ttcgacagcc agcggggcca ggcggtcgga tcagtgatac gcatgacagg ccgggttctc
    15241 ggtgtgtctt tgcatgtgga ggaagctgtt acccactatg agcctcctgt ccggagaacc
    15301 tgggaaacgc gcgatcagcc gcgcgtgctg gtgatcggac ggtaccgcat gggattcgaa
    15361 ctttccccgg ctgccaccgg tacccgcttg cgtgtctgga ttgactacag cgctccaacc
    15421 ggcctggcag ggcgctggct gggaaaactc ctgggccaag cttacgcaga gtggtgcgtg
    15481 acacgcatgg cccgggatgc aacgggattt ttaacaagga gtaatcatgt ctggaatcag
    15541 aaactggctg gtgatggcgg caatgctgtc aggatggggt gtcgcgggtg tctgcttggc
    15601 ggcagacaag gtgtatgtag cgaacgagga ggccgacacg gtcagcgtgc tggacgctgc
    15661 gtcgttcaag acgctcgcca aggtgcgtgt aggcgcaaag ccgcacaacg tgcaagtctc
    15721 accggatggc acgcggctgt gggtgaccaa caatggggag cctgtcgcag atgctgctgc
    15781 gcatcaaggc atgggcaagg acgaacatca ggccatgggt gttggtgccg tctgggtgat
    15841 tgataccgcg accgatgcgg tggtttcgaa agtaggggta ggcttgcatc cagcgcatgt
    15901 tgtgctgaca ccggatggcc gctttgccta cgtcaccaac ggcggcgaca acacggtgag
    15961 cgtgatagac gcctcgacgc aacgcccggt gtcaacgata gcggtcggga aatttcccca
    16021 cggcattcga ttcagcccgg acggcacgca ggcctatgtg gcaaacctca aggggggcac
    16081 ggtatccgtg gtggatacgg ccagtcaaaa agaagtgacg caaataccgg tgggcaaagg
    16141 ccctgcccag gttgggttca cacccgacgg gcgtctggct tttgtttcac tgtccgtgga
    16201 aaacgcggtt gcggtgatcg atcctgccac ccgcaaggtc ttgcgcagga tcgccgtcgg
    16261 aacggtcccc attcagttgt tcgcaacgcc ggattccacc accttgcttg ttgccaacca
    16321 gggcacgcgc aaaaaacccg gaaagaccgt cagcatgatt gatctgggaa gtttcgaggt
    16381 ggtcaagacc gttgaaaccg gatccggcag ccacggcgtg gcgatggacc gggaaggacg
    16441 gtacgcgtac atcacgaaca cctacgctgg atccatgtcg gtgatcgata tcaggaagcg
    16501 cagtgtggtg gcaaccgtac tcgttggcaa agggcccaac ggggtcagcg tgagaccgtg
    16561 acgtgcctgg gccggatttc agggctgatg cgtgacctgt gagaaagcgg gtcgcaggta
    16621 gcacagtcag aacgtgtcca gccacggctg aatgaatgca aaccagccac ccgggagata
    16681 gatcaccgcg ctcacaccct atacccgggg tgaaactttc cgggtgcgca tgcaggctcg
    16741 gcgcagcgcc ggaccgagtg ggcagggcgc aaaccggttg cgccattgca gcacgccact
    16801 gaagacccgt atcaatcctg ccaatgcgaa tacggctttc cggcactcgc tggcctcgag
    16861 ctcgtgtcta tttggccggc tcgatcgaca tcaccgtata ggcaccgttg accttgtcgg
    16921 ccgtgaagct gaccttgtcg ccggcctgga ccttgtcgag cagggccttg tccttgacct
    16981 ggaacaccat gctcatcggc ggcatgtcca ggttcttgat ttcgccgtgt ttgagggtga
    17041 tcttgccggc gtccttgtcg accttgcgga cttcggcttc agagacgggc agggcggcct
    17101 gcgcgaacgc ggccgaggtc acgagggtgg cgatcagtgt ggtgaagtgt ttcatgtcaa
    17161 ttcccttgaa tgcgctcagg cgcggtgata actttgataa acgcgggtcg tcccgctgcg
    17221 gctgatgagc accacgtcat acgggtcctt tcggttgccg taaatggcgc catccatgcc
    17281 gggcgagccg accggcatgc cgggtaccga cagaccgacg gcaaccgggc gttccttgag
    17341 caggcgatgc acctcgcgtg ccggcacatg gccttcgatg gcgtagccgc cgatcagtgc
    17401 ggtgtggcag gagccgagct tgtcgggcac gcccagccgc ttgcgcgcag cggcgttgcc
    17461 gctgtcgtgg accttgacat cgaaaccgtt ggcttccatg tggatcaccc agtccttgca
    17521 gcaaccgcag ttggggtctt tccagacttc gacctgcggc cgggtctggg ccatcaatgc
    17581 aggcgccagc agggaagtgc cggcgaggct gaccacggtt tgaataaaaa atcttctttg
    17641 catgatttct cctggagtga tgtttaacgg gatgccgcgg tgacactgat cttgccgatc
    17701 atgcccgcct ggtagtggcc ggcgatcagg caggcaaagt cgaactcgcc ggcgcggttg
    17761 aaggtccaga tcaggccacc gcgcttgcca gcgccgacgt gggccatgta aggttcatcg
    17821 tgctgcatgg tgggaaactt cagcatcagg gcagcatgtt catcgagctc ctttttcgtg
    17881 ccgatgacca gctcgtgcag cagcttgcct ttgttgcgga tgacaaaacg cacggtctcg
    17941 ccctgcttga agtccagctt gtcgggcgtg aaacgcatgt tgtcgtccat ggtcagggtc
    18001 acggtgcgcg tgaccgatct ggcgtcacct gcgatgcccc attccttctg ctccttgatg
    18061 gctgcagctg ccttgtgcgt tttcggatcg tctccatggg caaggctcaa ggtggtggtg
    18121 cccgcgagca gcagcgcggc gaggagaggg tgaaattttt tcatgaacat gtctctttca
    18181 aatcagtgct tgtgggtgga ggcggcggcc ggcgctgcgt tgtcggcagg cccggcataa
    18241 ccgcgcagtt gcgcataacg ggagatctgc gtcggtgtca gcagcgcggt ttgctgcaga
    18301 tgggtgtcca ggtgcgactt gcgcagcaag gcctgcaacc ggcctgtgct gtcggtgtgg
    18361 gccgccagcc gggtcgcatc gatctggcgc gacgcaaaag cctggtccag cgcacgttcg
    18421 gcctgcacca gttgcgcgcc caggtctcgg gcctccgcct tgtggcggtt catcagcgct
    18481 tcgctggcct gcttctgctc agcgctcagt tcgagttgcg cggccagctc cagcgtgtgc
    18541 atgggcccgg ggtagccgtt gagttcggcg gccttggcca gctccatccc cttgccggcg
    18601 agcaggtcgg cggtctgggc agacgagagg gtcttgatct cccgggtctg ctggccggca
    18661 taagggctgg cattgtgcgt gggcgcgtga acatgttggc caaatgtgcc tccagccccc
    18721 accaataaag cgctggcagc tatcaatttg agagtaaaac gtgtcgtcat gggtgttctt
    18781 tgaatcagtg gccggcatgg ccggaaggtt tgcggatctg gacctgtgtc acggggcccg
    18841 gtgaggtcgc cggcatggcc ttgctgccaa gacgtggccg cgtcgcgtcg ggcaatgcgc
    18901 ccgtccattc ataggcctgg gtaccggccg ggtgcttgta ccagcccggg tccttgtagt
    18961 caccgggttt ctggtttttg cgcaccttga gcatgct
//
LOCUS       DAIDFG010000017        18867 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_17_length_18867_cov_18.528599, whole genome shotgun sequence.
ACCESSION   DAIDFG010000017 DAIDFG010000000
VERSION     DAIDFG010000017.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 18867)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18867)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18867
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17_length_18867_cov_18.528599"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3507 a         6108 c         5966 g         3286 t
ORIGIN      
        1 tgcggctgag gtcaccggtg gccgtcagca ccagatcgcc caggcccgac aggccggtga
       61 aggtttcggc ccgcgccccc agggccaggc ccaggcgcgt catttcggcc aggccgcgcg
      121 tgaccagcgc ggcgcgggca ttgagcccga ggttcagccc gtcgcacagg ccggtcgcaa
      181 tcgccagcac attcttgacg gcgccgccga cttccacgcc gacgatgtcg tcgttcgcgt
      241 agatgcgcag ggtcgggctg tggaaggccg ccaccagcgc gtcgcgcact tcggcgtgtt
      301 cgctggcggc caccagcgcg gtcggttggc cgcacgccac ttcctgtgca aaactcgggc
      361 cgctgagcgc gcccgctctc aaattaatag ctacctgcgc ccgtatttca tgggccagga
      421 gcccaaaagg cttgaaggat gaggagatgg atggaacggc cggaagagcc ggaagtggcg
      481 cctcaaaccc cttgcacaac caggccaccg gcacgcgaat ctgctgcatg cgctgcaaca
      541 tgccgcgcag ggccgcgatc ggggtggcga tgacgatcag gtcctgctgc gccagcacgg
      601 catccagcga agcatcggag ccgcccgcca gcagcagggc atcgggtaag gcgatgccgg
      661 gcaggtagcg tgtgttgatc cgctgcgcct gcatcgccgc cagctgtgcg gcgtcacgcg
      721 cccacaaggt gacctgatgc cgcgcagagg tgccgccagc attggccgcg ctgatggcca
      781 gcgccgtgcc ccaggcaccg gcgccaatga cggcaatttt catgggaatg aaaaggccag
      841 gggcaatgcc agtaccaccg cgtccttaaa cgatgatctg cggggcttcc tgggccgccg
      901 aggctgccgc ggcctgttgc tgctcgaaca tggcctggaa gttgatctcc gccaggttga
      961 cgggcgggaa accgccgcgc tggatcacgt cggccacatt gccgcgcagg taggggtaca
     1021 cgatctgcgg gcaggcaatg cccagcaccg cgttgagctg ctcggccgcc atgttgcgga
     1081 tctcaaaaat gccggcctgc ttggcctcga ccatgaacac ggtcttgtct tcgatcttgg
     1141 tgtgcaccgt ggcggtcacg ctgacttcgt agaggccgtc ggcgatcggg gccgcctcga
     1201 cgccgagctg gatatcgacc gcaggctgct cctggttgag caggatttcg ggagaattgg
     1261 gttgctccag ggacacgtcc ttgaggtaaa cgcgctggat ctggaacacg gggtcaaggt
     1321 tggcttcggc catggtggtc tttcggattg cttgaaaaat gaaagcccgc tggacaacag
     1381 gcatgggaca ttatccccgt tcggataacc ggccccggac agcgcgcggc ccgggtggtg
     1441 cgagcggccg cctgtgcgtt cagccggcca gcaggggcaa caggccgcca cggccgtcca
     1501 gcgccatcag gtcgtcgcag ccacccacat gggtctggcc gatgaagatc tggggcacgg
     1561 tccggcgacc ggtgatttcc atcattttct ggcgctcgtc cggttgcagg tcgacgcgta
     1621 tttcctcgat ctcggccaca ccacgctgct tgagcaattg cttggcctga atgcagtagg
     1681 ggcaggaggc ggtcaggtat atcttcacag gggacatcga aaatctccgg gtcgggtcaa
     1741 tgggcagtgg caggttttca ggccttctca atgggaaggt tagcctcttt ccacgatttc
     1801 agcccgcccg acaggcattg ggcctgctcg tagccgagct ttttggcgat ggccaccgcc
     1861 cggttggcgc gcgcgccgga ggcacacacc agaatcaggg ggagtgcctt gtttttcacg
     1921 gtggcgggca gtttttcctc gaattgtccc agcggcaggt ttttggcacc ggcaacgtgg
     1981 cccgcggcga attccggagc ctcacagaca tcgatgacca cggccttctc acggttgatc
     2041 agctgcacgg cgccggcggc gctgagcgaa ccggctgcca tgccgctgga aatcaggggc
     2101 cagagcagca ttccgccgga tgccagcgca atggcgatca gcatccagtt gtcgatgaaa
     2161 aatttcactg taagtccttc ttgggttttg caattaagga gtgattttag aatgcaggga
     2221 gcgctgccgg ctttcccctg ggctttcccg gatgcctgcc gcagtctttg cccgcctttc
     2281 tggacacttt tttcgcctcg ggcaccctgc cggggcccgc cccttttcaa cttcacgaag
     2341 ctgcctcaca ccatgtacaa acttgtgctc atccgccacg gcgaatcaac ctggaacctg
     2401 gacaaccgct tcaccggctg gaccgacgtg gacctgaccg ccaccggcgt gcagcaggcc
     2461 gtcaacgccg gccggctgct caagaacgag ggttacgact tcgacctggc ctacaccagc
     2521 gtgctcaaac gcgccacgcg caccctgtgg cacacgctcg acgagatgga ccgcacctgg
     2581 ttgccggtgg ttcacagctg gcgcctcaac gagcgccact atggcgccct gcagggcctg
     2641 aacaaggcgg agactgccaa aaaatatggc gacgagcagg tcctgatctg gcgccgcagc
     2701 tacgacacgc cgccgccgcc gctggagccg aacgacccgc gctgcgaacg tgacgacccg
     2761 cgctacgcca ggttgaagcc cggcgaggta ccgctgaccg aatgcctgaa agacacggtg
     2821 gcccgggtgc tgcctttctg gaacgagtcc atggtgccgg ccatcctggc tggcaaacgc
     2881 attgtggtgg cggcacacgg caactcgata cgcgcgctgg tgaagtacct cgacaacatt
     2941 gccgacaagg acatcgtcgg actgaacatt cccaacggca ttccgctggt gtacgaactc
     3001 gatgccagtc tgaatccgct gcgccactat tacctgggcg acagcgaggc cattgccaaa
     3061 gccgaggcgg cggttgccgc ccaaggcaag gcctgagtac ccggaccgct gacggcgccc
     3121 tgctggcgcc cctgtgtcaa cgcgaggtaa aggtggcccg tttctgaaac attccggcac
     3181 gctggcaccc gagtggcgtc gactgcggga actgttggca agggcctttc tccaaggtgt
     3241 atattgtttc gaattctgtg acaactgccc caaaatcggg caaatgaagg tgtttatggg
     3301 tcagaaactc aagatagcag gctggatttc cttcggcgcg gtcgccggtg cgctgacgac
     3361 cgtgcaactg caggcggttg cgcggggcgg actcacgccg ctgccgctgg aagaactgca
     3421 gcagctggcc gccgtgttcg gcatggtcaa aaccgactac gtggagcctg ttgacgagaa
     3481 aaaactcatc accgacgcga tctcgggcat ggttgccagc ctggacccgc attcggtcta
     3541 cttcgacaag aagtccttca aggaattccg ggagggaacg agcggccgct tcgtcggcgt
     3601 cggcatcgag atttcgcagg aagacgggct ggtcaaggtg gtctcgccga tcgaaggctc
     3661 gcccgccgac cgcgccggcc tcaagcccaa tgacctgatc accaaaatcg acgacaccgc
     3721 ggtcaagggc ctgagcctga acgaagcggt caagaaaatg cgcggcgaac cgcggaccaa
     3781 ggtcacgctg atgatcttcc gcaaggacga aaaccgcacc ttcccggtga ccatcacacg
     3841 cgaggaaatc cgcacgcagt cggtgcgcag caaggtcatc gagcccggtt atgcgtggat
     3901 ccgcctgagc cagttccagg aacgcacggt cgatgatttc gtcgtcaagc tcgaacagat
     3961 ctacaagcag gagcccaacc tcaaggggct ggtgctggac ctgcgcaacg acccgggcgg
     4021 cctgctggac gccgccgtcg ccgtgtcggc cgccttcctg cccgagaacg tcaccgtggt
     4081 gacgaccaac gggcaactgg ctgaaagcaa gttcacctac aaggcttctc ccgagttcta
     4141 ccagcgccgc aatgccgccg atccgctcaa gcgcctgccc gcggccgtga agaacgtgcc
     4201 gctggtggtg ctggtcaacg aaggctcggc atcggccagt gaaattgtcg ccggcgcgct
     4261 gcaggactac aagcgcgcca ccatcatggg gaaccagacc ttcggcaagg gctcggtgca
     4321 gaccgtccgg ccgctgggtc ccgacaccgg cctgaaactc accaccgcgc gttatttcac
     4381 gcccagcggc aagtcgatcc aggccaaggg catcgtgccc gacgtgatgg tggacgaaac
     4441 gctggaaggc agtccgtttg cggccctgcg cacgcgtgaa gccgacctgg aaaaacacct
     4501 cgccagcggc cagggtgccg aagtgaaaga cccaggccgt gaaaaagccc gcgaggaagc
     4561 gctcaagcgc ctggaagaag agtccaggaa gagccccgaa cagcgccgcc cacccgagtt
     4621 cggcagcgac aaggacttcc agctcgcgca ggccatcaac cagctcaagg gccggcctgt
     4681 aatgatcagc aagaccatgg tcgagcgcaa ggaagagaaa aaggaaaact gaccgtgtct
     4741 gcccgccagg gtgctggtcc ggacgggccg gcagcctggt tgccccgcac tgcccgggtc
     4801 acgtccgccc atgaacgaca cccagctgct gcgttattcc cgccacatcc tgctcgacga
     4861 actgggcgtg gaaggccaga acaccctgct gtcgtcgcgt gcgctcattg tgggcgctgg
     4921 cggcctgggc tcaccggttg cgctttatct gggcagcgcc ggggtcgggc gcatcaccgt
     4981 ggtggatgac gatccggtcg acgccaccaa cctgcagcgg caaatcgccc acacgctggc
     5041 acgtgtcggc cagtacaagg cgcactccgt gcaacaggcc gtcgccgcca tcaatccgga
     5101 cgtcgaggtc actgccgtca ccacgcgcgc cgacgctgcc gtgctcgaag aacacgtcca
     5161 gcaggccgac gtggtgctgg actgcaccga caactttgcc acgcgccagg cgatcaaccg
     5221 agcctgcgtg aaacaccgca aaccactggt atccggtgcg gccatccgct tcgatggcca
     5281 ggtgacggtc tacgacccgc gccagagcac ctcaccgtgt tatgcctgtg tctttccgcc
     5341 gacggagacc gtgcaggagg cccagtgcgc caccatgggc gtgtttgccc cgctggtggg
     5401 cattgtcggc acggtgcagg cggccgaagc gctcaagctg ctgtgcggca ttggccaggc
     5461 actcacgggg cggctgttga tgctcgacgc acgcaacatg gactggcatc acgtgcgggt
     5521 cgcacgccat ccctcctgtc cggtgtgcgg cggcgcgtcg gcctgaacgc cgggcgcccc
     5581 tgaggcgcca cggcctattt ggccgccgag ctggccgccg cgggcttgtc cggcggcttc
     5641 accggactgg ccactgccag cacccgttcg cagtcggcat ctttgccagg tccggtttcc
     5701 accgtggcgg ccaacgcgag caagggattg atcgcgccga gcagcagtgc aatgccggcc
     5761 cggcccgcca gcgccgcctt gtcggggccg gccgccggcc gggcaaacgt gccggtgatt
     5821 ttcaagggag agcgcaggct caggatgctc tggtccttgg gctgcgggta cagcaccagg
     5881 tccagcgttt catcggtcag gctgatctgg cccgtgccgt tgatcagcgt gtcggaggtg
     5941 tccagcagga tggcccggct ggtcatcagg ccctgcttga catcaaatgc cgcggcagcg
     6001 caacgcaggg tgacattgcg gtcgccgctg gccagaaact tgatgatctc gccgccgtcc
     6061 agccccatga actcgagcag gatgttgctg acctggcccc ggcccatcag cagggccacg
     6121 ctaccggagg acgagcccag aacctgcgcg ccggagttgc cgcggccggt caggtcgatc
     6181 cgcccgttga gcttgcccag gctgccttcg gtgcgttcga tgcgcggaaa cagctggttc
     6241 agctgcagcg tgcgagcttc gagtctggcc tgcaccagcg cgggtttgct gttggcgtcg
     6301 atacggacta ggccggccag ccgtccaccg gcaaccccca tgtccagcgg atcgagctgc
     6361 aacacgccgt ccttgagcag aatgcgcgcg tcaaagcgat ccagcggaaa ggcgccggcg
     6421 ttcttgacat ccaccacctt gtagttgacg tcggcattca tggttttgag ccgctcgaaa
     6481 tccagtgtcg ccgtcggcaa caccttgccg gccgcctgca cctgcttggc ggccgaggaa
     6541 ggcttgcctt tggcaacggt tttggtcgtt gcgcgtgaag cacctgtcgg cacaccaatc
     6601 agcgggccca ggtcctcgaa atcgagcgat ctggactgca ccttgcccga cagcagggcc
     6661 agctggcccg agcggtcgta ggtgagatcg cccgacaggt cggatttgcc cagacgcccc
     6721 tggatggcgg ccaccttcca cacggccccc tgcttgccga ggtggccgcg cagggcatag
     6781 gtgggggtat ccggcaaaac gacgcccagc aggttgtaga ggtcggccag cgtgcggccc
     6841 ttgaggtcga aattggcatc caggccgtcg agcgacgcca gattgccgat gctgcccctg
     6901 gcttgcagcg ttgtacggcc tgcactggca ttgacgtcca tcggaaacgg gttgatctgg
     6961 gtgtcgctga gttgcagcac actgccagcg cgcccctggg ccgaaaagac ctcttttttc
     7021 caggtaccac gcgccttgaa gctcagcggc atgtcgcggg ccgccgccgt tgttccggct
     7081 tcggcctcac tcgctgcgac catgccaaac tcggttttga tgtcggcccc ctgcgcttcg
     7141 gccagataat tgagtacgcc ctggtccacc agcagctggc caacaagcgg cacggtagag
     7201 gcatcggacg tgtccttgcc caatgcccag gtgcgccggc catccggctg gatttgcagt
     7261 cccagcaccg gctggctcag gctgacgcgc gggagttcaa cccgtccgcg cagcaagggc
     7321 cagaaccgga tgtcgaactc ggcggactcg gccttgacca ggtacgggtc gcgcgcccag
     7381 tcggggttgg cgaaggtgat gccatcggcc ttgaccgtga tggtgcgccc cagctgcacg
     7441 tccagccgcc gggtgatttc gaacgggcgc ccggtctggt cactgacgta gcggttcacc
     7501 ggcccgcgca accagtccca cggaaaaaaa acaatcacca gaatcaggac cgccagaacc
     7561 agtgccgcgc ccgccacgag ccgaccccat gttgggagga ggcgaaggcg ggagacggtg
     7621 gaagcgacac gggtcatggg ggcggcctgc gattcttgat ttgggaagac cgggagaggc
     7681 ccgacacctg caagtggttg gcaccgcgct gtgaaagcga aggggaaatc agctgacagt
     7741 gaagtatctg ccggtaaccg ccttcacacc gtgggccaca gccatcgatg actgtcagtg
     7801 cctgcctaca gttgcatcgt aggaaacttc ctacaaacca agcacgctca ccctgtcaga
     7861 atcggtctga agacatggat acattagtta acagatagat cggtgtgcgg ccaacagccg
     7921 ttgtaaccgt ttgaaaaatg ccgcggtcaa ggatggcaga ccgggattga agctcagtgg
     7981 gactaaaaac ttatatcgtc gaagacaacg ccaccatccg cgacaacctc ctggctacgc
     8041 tcgaagagct ggccggtatt gacgcagtgg gtacggcgga gacagaaaat gaaggcaagg
     8101 cctggctgct ggcaaacggt cagcaatggg acctggcgat cgtggacctg tttctcaaac
     8161 agggaagcgg actgggcgtt ctcgaagcct gccgcaaccg ccaggtgcat caaaaagtca
     8221 tcgtgctgag caattacgcg acggccgaca tccgcaagcg ctgcgcccag ctcggcgtgg
     8281 acgcggtctt cgacaaatcc aacgagatcg acgcactgat cgactattgc atcgagcagt
     8341 gcaggtcgat ccatgccggc cagccccaga aatagggccg gcggcttgcg gcaggcgctc
     8401 agtcaatcag cttgttcttg agggcgtaat acgtcaggtc gctgttggac gcgagactca
     8461 ttttctccat cacgcgggtc cggtaggtgc tgaccgtttt gacactcagg gacagcgcct
     8521 tggcaatgtc gcccgcggtt tcgcccttgg caagcttgag gaacacctgg aactcgcgct
     8581 cggaaagctg ctcgtgaggc gtggcatcgt ttttccggtt cagctgctgc gccagcaact
     8641 cggccactgc cggcgtgatg tagcgtttcc cgagcgaaat cacgcggatc gcattgacga
     8701 tctccatcgg atcacactcc ttgttcaggt agccgctggc gccctggcga atcaggttga
     8761 ccgcatagtg ctcttccgga tacccgctga gaatcaggat gccgacatcg ggggccttgg
     8821 cgcggatcat ggccagggca tcgattccgc tctggccagg catggacagg tccatgacca
     8881 gaatgtccag ctcgacattt cgcaccaggt caatggcctc gcggccactg gcggcttcac
     8941 cgacaaccct gaaatcgacc tgatccgaaa agaactgctt gagcccggag cgcacgatgg
     9001 catggtcatc cacaattccg attttgatca tttcatgtcc tcttggttag gctgacatcg
     9061 ccaaacggca caatgcagca acacggcctc gcgcaattgc gggcttctgt cttcagtagc
     9121 gattattcac gaattattga tccatgccga acggagatca ggatgagatg gtttgcaatc
     9181 cccaaaatgg caatcagcct gccgctggcg gtgctggcag cggtcgttct gatttccatc
     9241 aacgaagcgg gttttcgcct gtccacccag gccgttaccc gcattgagca gtctcaggct
     9301 acccgtgggg ccgtccatct gctgctgcag cagatgctgg atgccgagac cgggcagcgc
     9361 ggctacctgc tgaccggcga tcccaaatac ctggaacctt acgacgcggc aaccgccgcc
     9421 ctcaaccaca ccctggacac cctgcgcgga cactacaccg cctatcccgc ggagctggcg
     9481 gagtttggcc tgctgtcgcg cagcgtgtcg cgcaagctgg cggagatgga cctcagcgta
     9541 cgcatgcgca agcagggcaa cgaggaagcc tggaagtttg tgctgaccac cgacgtgggc
     9601 atggaccaga tgaacgccat ccgcagccag atcgtcaagc tgatccaggc cagcaacagc
     9661 aagatgggca cagcccaggc ccagatcacc cggtcgttgc agttgtcacg catcggcatc
     9721 gccctgatcg ctctggccgg gctgctggcg ttttacatgt atctgcggca aaccggggcc
     9781 ctgctggacg ccggcaggcg cgaacaggaa gcactgcagc gcgaacgcga ccagctggac
     9841 aaggaagtgc gcgatcgcac ggccacgctg gccgagctgg ccacccacct gcagcaggtg
     9901 cgggaagatg agcgtggcca cctggcacgt gaactgcacg acgaactggg cgccctgctg
     9961 acggcagcca aactcgatgt ggcacgcatc aaatcgcggc tcggggtcat gtcaccggaa
    10021 gcggccgaac gcatgcagca cctgatcgag acgctcaaca gcggcattgc cctgaagcgc
    10081 cgcattgtgg aggacttgcg gccctcgtcg ctgtcgcacc tggggctggt cgcctccctc
    10141 gaaatcctgg cgcgcgaatt cgaagaccgg tccggcctga gcatcatcac cgacctggag
    10201 acggtggagc tcggcggctc cgcacaactc acgatctatc ggctggtgca ggagtcgctg
    10261 accaacatgg gcaaatacgc cgatgcaaaa caggccgaaa tcagcctgca tgattttgac
    10321 ggctacatca tggtcgaggt gcgggacgac ggcaacggct tcgacaccac gcaaatcaag
    10381 ggatcgagcc acgggctggc cggcatgcag caccgggtcg aagcggccgg cggacgcctg
    10441 accgtggcct ccagtcccgg ccagggcaca cgcatctccg cagtgttgcc caaatcgctt
    10501 taagcgccgg cgttcccgat ggcggggcca caacaacaaa ccccggcccg ggcccagcca
    10561 catgcgcaga agcgcctatc ggcagcgtcc tacacggcat gttcatgcca gccgacaagg
    10621 gcatccggca ctcaccgatg aatggatggg gcttcgacgt ttaaccttgc tacaggtgtt
    10681 tcatttgcga gcgccacgta catcatgggg cgcgcccctg caaggagatc gacatgctgc
    10741 actattccgt tgttttcctc gtgatcgctc tgattgcagc cgtgttcggt ttcggtggca
    10801 ttgccgccgg tgccgtcgag attgccaaga tcctcttttt catctttgcc atcatggccg
    10861 tgatcagctt cgtggtcggc ctgatgaaaa gaaagtgaat tcccatctcc cggtccaggc
    10921 gcagattgcg ccggatctgt ctttttccat ttcccaagga accccatgaa aaacccgctc
    10981 tccagccacc cccaagccca agacacgctg tcgggtgcac gtcatatgtc cgagcagatg
    11041 ctcaacggtg ccgagagggc catggacagc acccgcagcc tggccaacga cacgctggac
    11101 cgcgccgagg acaaggttcg acatttgcgc ggcagcatcg acccgctggt cgacaagctg
    11161 gccacccagg cgcagaaact ggcacgccag agcctggaca tggcctcgga ggccagcgac
    11221 cgcgcgcagc gttccatgca ccgctatgcg gacgcaacga cacgttatgt gtccaatgag
    11281 ccggtgaagt ccgtcctgat cgccgctgcg gtgggcgccg ccgtcgcctt gctggtggcc
    11341 tcgaccgtgc atcgcgacaa gcgctgattt tcgcggcttc gccccctctt cacgaccact
    11401 gaggcttcat gctgcatccg ctgttttcca ttctcgtgcg acggcccgac ctggtggttg
    11461 atcacctgtc ggcctatggc gccctggtcc acgaggaagc ctcggaagcc gggtcggaac
    11521 tgcttcagcg cgggctggcg tggggtctgg tcatcctgtg tgccggcgtg tttttgacgc
    11581 tggccggtgt ggccctgatg ctgggcgtgt tgttgaacca gtttcactgg gtgctggttg
    11641 ccattccggc ggtcatgctg gtgctgcttc tggccgcctt cagcaaggcg cggcagcccc
    11701 tggccgcgac ccgttttgac gaactcaagg cccagctgga ccgcgatgcg caggcattgc
    11761 gcgcagcagc ctgaccccga tgatgaacga caacaccgtg ctttctcccc gcgaacgcct
    11821 ggacaagagc cggcaggcaa ttgttcgcca gatggggcat gacaacgcca cggcggtcga
    11881 gcatggcggc agcgacaccg aaattgatgc gtcggccagc acctggtcgc tcgtcaaaca
    11941 gacggcctca tcgtggtggc atggccaccc ggccagcctg gctctggatt ttgcccgacc
    12001 gaggatgcag cgttttgccc aggagcagcc cctgaagctg ctggccattt ctgcgggcat
    12061 gggcgcagcg gccgtgttgc tgcgtccctg gcgtctgata tcgctgaccg gcctcgtgct
    12121 cgcggctctg aagtcgtccg aggtcaccgg cctggccagc tccctgctgg cctccggcac
    12181 ccggaacgac cgctttcgat gaattccatt cgcaaaccga ccggcgtggt gccgtcagtc
    12241 ggccagcttc tcaactctca gcaccaaaac atatttataa acttcaagga aacaccatga
    12301 aatacgctcg cgcaattgca ttcgccgccc tggccggcat caccatcatc agcgctggtt
    12361 gcgcagttac ccgcggtcag gaaactgccg gctcttacat cgacgatgcc gccatcacca
    12421 ccgctgtgaa ggccaagttt gcttcggaca aaacggttgc cgccaccgcc atcagcgtgg
    12481 aaacgctgaa cggcacggtc cagctttccg gctttgccaa atcggccgct gaaaaagccc
    12541 aggctgaggc cattgcacgc ggcaccaaga acgtccgcgc tgtgcgcaac gacatcgtcg
    12601 ttcgtccttg aactagccac tatggggtaa cacaggccgg ccctgccagc ctgtcaacgg
    12661 ggtccggttg tctgcaaccg gaccccgctt tgccttgtgg ggatccatgc gcgatgcgct
    12721 gccagttggt cgacgcaccc tgagcgcaga gggtcacggc ccggcgcccc cgcttccagc
    12781 aaacgcccat aaaaaagtca ctggcccgcc acccgaaggc gcgcgccagt gaccggcttg
    12841 ggcggaagag tgaattaacc gggtgtgacc accagctggt tattcaccga cgtcacgcct
    12901 ttgacctgcc tggcgatgtc tgcggccttg tctttcgcta cagcgctggg cgccgggccg
    12961 ttgagcgtca ccacgccatc cttggtgtcg acattgatct tgatcgcgct cagatccggg
    13021 tccttggcca gtccagtgga gacggtcgcc gtgatggtca cgtcgtctat tttttcaccc
    13081 atggtggtgc tggcctggct gcccgtggct tgcgcgtcct tggatgcttc cttcatggtg
    13141 ttgccggcat ccttggccgc ctgctcggtc ttggcgatgc cggagtcgat cgactggccg
    13201 acggtcttgc cgtcatccgc cttgttgcag gccgtcagcg ccagcagcac ggcagcggaa
    13261 tacgccagcg ccagaagcgg gcgccgcgcg tgaagagggg tcgggttcat gatggctctc
    13321 ctggtgtggg atgagattcg caagcggtgc gttcagatct tgtcgatcag ttttttggcg
    13381 tcgtccttga cgctttcctt ggcgtcgccg taacccgcct gggttttgcc cttgacctgg
    13441 tccgccagac cttcggcctc cagcttttca ttgccaacca cgcggccagc cacttccttg
    13501 gctttgcccg cagcctggtc cacacggcct tcgacttgat gcttgttcat ggtgcttctt
    13561 cctttgaaaa tggggtgcgg cccgcctgga tggcgagcac atccccactg taggcaggcc
    13621 ggttggggcg gcccgtcagc gcctgcggaa ttttttgtag gacggcaggc attcactgcg
    13681 cagcggcacg tcaagggtgg caggcgccgt gctgtttgcg gaaaaggcgg ggcggcagcg
    13741 cctgcggatc ggcaaacagc ccacgctgcg ccgaccgggc ctgcgcctgc aacttcaggt
    13801 agggcccgcc ctcacgccca taccggtccg accaggcatg gccttgcgcg accatccagc
    13861 cggccatgtc ttccccaccc agatgcacgg tcgccacggc gcggccgtag tcgtcatgac
    13921 ggtgcgttct cacgaccacc gtgcggcgcg ccacgcggct ggccagcgct gcacgcgcgg
    13981 cacggccgcc ggcctggcag atctcgggtg catcgatgcc ggccacgcgc agcttcaccg
    14041 gcttgccgcc tccggcgggc cggacccaca gggtgtctcc gtcggtgacc cgagtgactt
    14101 tcatgctctc cgcccatgca ggatgggcgg aagcggctat caaaaatata gcaatcttga
    14161 acgcggtcat ggtcatgcag taacgatcca gccctcaatc gggtcgacat cactgggcag
    14221 gccgtaacgc gcgacgccct gcgcatgctg cgcttgggcc cacgcggcct gcatcaggca
    14281 gagcgccgcg tccagactgt caccgctggc gtcgtcgacc agggtgtcac gctgcgcgtg
    14341 gctgagcttc aggcgcaggc cgagccgggt ctggcccatt tccagcgcgg tgatcaggtc
    14401 cttgcgcgcg atcagccggt ccggcgtctg cttgagccgg tcatcacttt tgtaggatcg
    14461 ccggcccagc acctcgcgcg cgagaagccc cgggtaggct tcgagcgcaa cgcgcgacag
    14521 gtcgcccgcg tgcaggcccg gcaggtgaac gcccgctgcc agcaactgcg gcacacccgc
    14581 gtgaagcatg aaggccaccg gcggattgat ccatttcatg gacgggctgg aaccggcggg
    14641 aatatcggtg gcgcggtgcg caaacttgcc gcccacagga cgcgccgcgc agaaggccgc
    14701 aaaagtctga cggatctctt cccggctgag gctggcgtag tgggccatca gttccgtcca
    14761 agtgcccggc cagccgagat gctcgaccag ctcgcgcggc aggccgaagg gaaagtcgaa
    14821 cgcacccagc cacgcgcgcg gctggttcag ccagtcgccg aaggcgtcca gcgacgcaat
    14881 atgctccagg ccggtcagca acacgcgccc gccggaaagt gatccgctgg ccaggacaat
    14941 cggcttgcgg cgcgtcgggc tgctggagaa atcgcagccg atcagcaggg gcagggtctg
    15001 aggcatggcc gcttccgttc agcccagggc gagcgcggtc acgcccgcgg caatcaggcc
    15061 ggcgcccagc aggcgctgca ggcggtcgcc ctcgccgagc aagtgcccgc cgatcagcgc
    15121 ggcgaacagc atggagactt cccgcgccgg tgccacatgc gacagcggcg ccacctgcat
    15181 cgcatacagc accagcacgt aggagaccgg gctgatagcg ccaaccaaca gcgcgtacct
    15241 ccattgctcg cgccacagcc gcgccgccgc cggcctgtcg cgcaggacca ccggtgccag
    15301 cagcgccagc cgcacgaaat tgcccaggta gtcgagcagg atgggcgaca tcagcatgaa
    15361 cttgaccgca taaccgtcca ccacggtgta gctggcgatg aaagccccgg tgagcaggcc
    15421 gtacagcatg cccttgtgca cgcggcggcg ctgggcggga tcctgcgcgg cacgcagcag
    15481 gccgggcccg ccggcaatca ggaacacacc aaccaccaca ccaacaatgc ctgtcagccc
    15541 cagcgccgag atctgttcgc ccaacagggt gatcgccacc agcgaggaca gcagcggccc
    15601 ggagccgcgt gccagcggat agaccaccgt cagatcggcc ttgcggtagc cgcgcagcag
    15661 gatcacgtaa tacagcacat gcaacacacc gctgacggcg ataaagaccc attcgcgcca
    15721 gccccagccg ggaatcacgc cccaggcggc ccatgcaccg accggcgccc agaccagcat
    15781 caaggtcacg ctggtaaaaa aggcgaaacg ggcgtcgccg ccggcttttt tggccgcaat
    15841 gttccagctg gcgtgaatca ggccggcaag caggaccagg gcaaaagcgg acagcggcat
    15901 gacgcaagcg gccggcggcg cgcatgaacg ccgtgcgccc gcgcagccgc ctggccagga
    15961 gaactcagac cttgccgcag atggaggcgg tggccatcag gtcttccagc aaggccagcg
    16021 acaccttgcc cgacaccgaa taagggtcga gctcggcctt ctcggagtat ttgagcagga
    16081 tggaggtgtt gatgcgctcg acattgacct ggcccatggt ccagccgcca aaacgccgct
    16141 cggagatttc ctcgtaatgc agcagcacca cgtccttgtg gcgcggatct ttctggatgt
    16201 ggccgtagag gtcgctgatc tgcatgcgtc cgccttcgag ggcctgcagg aaaatgccgc
    16261 cgccgtagca gaggatgccg gtgatgccca tggccgggtt gtggctgcgc gactgcgcaa
    16321 gaatggattc ggtgagatcg ctggagccgg ggttgaccga gcggctggcg taaagaaggc
    16381 gtaccaacat cgtgtgtcct tagctgctgc tctttttcgg gatgagtgac aaaaattcgc
    16441 ggcgcaggtt cgggtccttg aggaagaccc cgcgcatgac ggaattgatc atcttgctgt
    16501 ccaggtcctt cacaccgcgc cagcccatgc agtagtgact ggcctccatg acgatggcca
    16561 ggccgtcagg ccgggttttt tcctggatca ggtcggccag ctgcaccacc gcctcttcct
    16621 ggatctgcgg gcggcccatg atccactccg ccaggcgcgc gtatttggac aggccgatga
    16681 cgttggtgtg ttcgttgggc atcacgccta tccagagctg cccgatcacc gggcagaaat
    16741 ggtgcgagca ggcactgcgc accgtgatcg gaccgacaat gatcagttcg ttgaggtgct
    16801 cggcattggg aaattcggtg acagtggggc cggccacata acggcccttg aacacctcgt
    16861 tgaggtacat cttggccacg cggcgcgcgg tgttgttggt gttgtggtcg cgctcggtgt
    16921 cgatcaccag gctgtccagc acgccctgca tcttgccttc cacctcgtcc agcagctttt
    16981 ccagttcacc cggctcgatg aaggcggcaa tgttgtcgtt ggcattgaag cgttttttcg
    17041 ccagggcgag gcgttcgcgg atcttgacgg agaccggcgt gccttcgtca agaagggcgg
    17101 gggaatcagg cgtattggat ttcataagca tggccagatg ttaccagtct gccccaaaac
    17161 ggccatgccc cttttttaca agggttttag gcctccagcc cttgatggac gtgcgctgac
    17221 agctatgaat taaggagcgt tttagcacct gccggggagg agccccgtca gcaggcgaat
    17281 cggcacgggc tcaataacgc tgaccggcga tcagcagggc caggcgcatc agggcgtagg
    17341 cgatgtagtc cagcgcccgc tggcgccacg gccggcggct gtaaatgtcc ggatccagcc
    17401 gcacgccgct ggccgtcatg gccagcacca ggcgctcgcg caaggcctgc gcaaagccgg
    17461 ggtcctgcac caccacgttg gcctcgcgcg ccagcagcag gctcagggga tcgaggttgg
    17521 acgagccgac agtggcccag cgcccgtcga tcaccgccac cttggcgtgc aggaaaccgg
    17581 cgtcgtattc atggatctcc acacccgccg ccagcagcgc gccatacacc ggccgcgccg
    17641 catggtattg catgaagtat tcgtagcggc cctgcagcag aaggctgacc ttgacgccgc
    17701 gccttgaagc ccggatcagg gctcggcgca acttcccgcc cggcacgaag taggcattgg
    17761 caatgatgat ctcgtcgtgc gccttgccaa tggccttgcg gtaggcgcgt tcgatggtgc
    17821 ggcgattgag caggttgtcg cgcagcagca ggcccgcggt gtcgctgcgc ccacgcgtcg
    17881 ggcgaaagga ggcgccgtga cccagcccgg cggaccggcc accgtaaccg gcagccttga
    17941 cagcctccca ggcggcgagc aggtggcgct ggcgcgcgct gtagccggcc tccacacgcc
    18001 accagagcag cgccatggcg tcggaggctt ccaccgccag cggcccgatg agcgccacgg
    18061 caaagtcgaa acgcggttcc gtcagcggcc cgtggttggg gtcgtaaaaa tcgtccagga
    18121 tattgatgcc gccgcaaaac acgatgtgtt catcaaccac acacagcttg cggtgcaggc
    18181 ggcgccagcg ctcgggaacc aacagcccca gcgcagcaaa ccggccgcgg cccagcggcg
    18241 cgtaaaagcg ccattgcacc ccggcctggt cgaacttctc atgccattca ggagaaatgc
    18301 ggtcggtgcc tacgccgtcg accagcacct gaaccagcac accgcggcgc gccgcgcgca
    18361 ccagcgcttc ggcgacgtcc gccccctgcc catggacgtc gaagatatag gtttccagct
    18421 gcacccaggc cgtgcccgcg tcgatcgcct gcaccagcgc cgggaacagc gcctgcccgc
    18481 cctgcagcaa ttgcagcgcc tgcggatcct ggttttgaag tgtgggcatg gttcagggct
    18541 tgtgcaggga gcggaccatg tcgggcggcg ccggcgcgat gccgcgccgg gcaggcaaca
    18601 gccctttacc ccgcaaattg tggcagcatc agcacggcct ctgcgttgga gggggaaaag
    18661 caccggtccg ccgcttcccg gtaaggcagc cactcccagg cacggtgttc gcgggggctg
    18721 agcgtgaccg gcgtgaccgg cggcacacac aggctgaaga catgttcggt gttgcgtgtc
    18781 acgccgggcg cgtaacgatg ccgccagacc gggtagatct cgtagacgtt ggccagcgcc
    18841 cagtcccgga aatcttccgg ccgcact
//
LOCUS       DAIDFG010000018        18642 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_18_length_18642_cov_17.025502, whole genome shotgun sequence.
ACCESSION   DAIDFG010000018 DAIDFG010000000
VERSION     DAIDFG010000018.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 18642)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18642)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18642
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18_length_18642_cov_17.025502"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3501 a         5807 c         5781 g         3553 t
ORIGIN      
        1 ctcctggact tccgccgaac gcgtcaggct ctggtagttg ctgaagggaa cgtcgtggtc
       61 ctgcgcatat ttttcgacgt tctcctggtc gatcatgatg atggcggtca ggtagggacg
      121 tttgtcgccg atcaccacgg catcggtgat gtagggcgag aacttcagtt cgttctccag
      181 ttcgctgggg gtcacgtttt tgccgccggc cgtgatgatg atgtctttca tgcggtcagt
      241 gatgcggtaa aaaccttcct cgtccacgag accgacgtca cctgtgtgca gccagccgtc
      301 ggcatcgatg gtttctgcgg tcttctccgg caggttcagg taacccatga agacattggt
      361 gccgcgcacc aggatttcac cggtcgccgg gtccagcctg acctcgttgt aaccggccgc
      421 cgggccgatg gagccgggct tgatgcggct ggccggcaca ccggtcgagg caccgcaggt
      481 ctctgtcatg ccccagacct ccagcatggg cacaccgagc gccatatacc acttgaccag
      541 ttccggtgag atcggcgcgg cgccggtcac gcagaagcgg gcgcggtgga tgcctatgag
      601 cttgcgcgcg ttgtccagtg cgatccaccg ggcgatggtg aacttcagct tgagccagct
      661 gctgaccggc tggccggcca tgacccgctc cgcgatgtcg ctgcccacgc caatgcccca
      721 ggcgtaagcg gcctgctgca ggcggctggc ttccttgagc gcgatcatca cgcccgagta
      781 gaacttttcc cagacgcgcg gcacggcagt aaacaccgtg ggcgcgattt cgcgcacatt
      841 ctcgggaatg gtctcgggat tctcgacaaa gttgagtttg gcccccgtgt acatggcgaa
      901 gtactcgccg cccatgcgct cggcaatatg gcacagcggc aggaaacaca tgcgttcgtc
      961 attttcgtcc tgtgccacca gggtgttgta gccgcgcgcg gtgaacacca ggccccggtg
     1021 gctgtgcatg gcccccttcg gttttccggt ggtgccggat gtgtagacca ggatggcgag
     1081 gtcttcgggc ttgcaggcct tgacgcgctc ctcgaccgca ccggggtgcg cagcaaggta
     1141 gctgcggccc agttcgcgca ggccggcgag actgatgaca tccgcctcgt cgaggtcgcg
     1201 caggccttcc atgtcaaaca ccacaatctt gcgcagcccg ggaagctgtg cccgcacttc
     1261 cagggccttg tcgagctgct cgtcatcctc gacaaacaga atggtcgtgc gcgagtcttc
     1321 gcacagatag tgcacctgtg aagccgcatc ggtcgggtag atgccattgg ccacaccgcc
     1381 gcaactgagc acggcgaggt cggccagtac ccattcgatc accgtgttcg agaggatgga
     1441 ggcgcattca ccgggcgcaa aaccaaggct gatcaggcca ccggcgatct cgcgcacggc
     1501 ttcgccggtc tggttccagg accaactgcg ccagataccg agctttttct ggcgcatcca
     1561 gaccttgtcc tggcgctgcg ccacaccgtt ccagaacagt tccggaatgg tttcgccttc
     1621 gagcaccacg cgggtttcag gctggatgtg ggaaagatcc cataggttgc tcatacgttt
     1681 ctccacaggg ctgtcatccc ggatctgatc cgggatccat gcctcgcgcg caaatgcagt
     1741 tcgggggaga tggattgcgg atcaagtccg caatgacaca agcaggagga tgcaactcgg
     1801 aagtacatat tgttcaacgc cacgttttct ttttcttcca gcgccgctcg ctgcgcacac
     1861 cgtcttcctt catgcccagg tagaactcct tgatgtcttc cttttcccgc aggcgggcac
     1921 aggtgtcttc catcacgatg cggccgtttt ccagcacata gccgtagtcg gaggcattca
     1981 gggccatgtt ggcgttctgc tcgaccagca aaatggtggt gccgcgttcg cggttgatac
     2041 gcaccacgat ctcgaagatc tccttggtca gcttggggct gaggcccaga ctgggttcgt
     2101 ccagcaggat caggtcgggc gaggccatca gcgcgcgcga gatcgagagc atctgctgct
     2161 ggcctcccga aagcaggccg gcgtcctgca gcgcacgctc gcgcaggatg ggaaagtagc
     2221 cgaagaccac ctcgatgtcc cgcgccacgc catcgcggtc cttgcgggta taggcgccca
     2281 tgagcaggtt gtcgcgcacg ctgagcagcg ggaagacttc gcggccttcg ggcacatggc
     2341 tcaggccctg ctggacgatg taggccgggt cctgcgccgt gatgttgccg cccttgaatt
     2401 cgatcgaacc cttgcgcggg tcgatgatgc cggaaatggt tttcaggatg gtggttttgc
     2461 ccgcaccatt ggagcccagc acggtcgcaa tttcgccgcg gcgcacctgc agactgacgc
     2521 cgcgtatggc cttgatgggg ccgtaggagc tttcgacgtt gagcagcttg agcacgggct
     2581 ggtcgctcac agggggtggt acggcgctca tgcgtccacc tcgcttcgca ggatggggga
     2641 cttgtgttcg cgccgcagcg aggacacatc atcgatggag ccgagatagg cctcgatcac
     2701 gccggggtcg ttctgcacct cgcgtggtgt gcccatggcc agcacctcgc cctggttcat
     2761 cgcgagcacg cggtcggaga ccttggagac cagcgtcatg tcgtgctcga ccatcagcac
     2821 cgtgatgccc agttcgttct tgatgtcctg gatccagaag gccatgtcgt cggtttcctc
     2881 cacattgagc cccgaggaag gctcgtcaag cagcagcagt ttgggttcgg tgcacagggc
     2941 ccgggccagc tccaccacct tgcgcacgcc gtagggcagg ccggccacat aggagtcacg
     3001 gtggtgttgc aggtccagca ggtcgatgat ctgctcggcc ttttcacgcg cctgaatttc
     3061 ggcttcgcgc gttttcttcg tgaagaaaat ttcgctccac aaaccggtct gcctgtgcgt
     3121 gtgccggccg atcagcaggt tgtgcagcac ggtggcatgc tcaaacagct cgatgttctg
     3181 gaaggtgcgt gcgataccga gtgcggcgat cttgtgcggt ggctggtcgg tcagtttggt
     3241 gcccatgtag tcgatgtcgc cagatgtcgg tgtgtagatg cggctgatca ggttgaagac
     3301 ggtcgtcttg ccggcgccat tggggccgat cagggtgaac acctcacctt gtttgacatc
     3361 gaagctgaca ttgttcacag ccaggacgcc tccaaagcga acactgagat tttttgcgga
     3421 cagcaggggc tgcatggcgg atggttgtat ggcgctcatt tcaggcgatc cgacttttga
     3481 aaagatttct ggcgcttgaa catgcccttg cggtagaagg gaaaggtctg caggtaggtg
     3541 cgtatcttga gccaacgccc atacaggccc aggggttcaa acaggacaaa accaatcagc
     3601 accaggccgt agaccaggcc ctgcaggccg ggcgcctgcc cgatgaagtc cggcaggtag
     3661 tccttgacca gcgcaatggc ctgcggcatc gagatcagga agatggcgcc aaggaaagcg
     3721 ccgtgcaccg accccaggcc gccgaccaca atcatgagca gcaggtcgat ggactgcagg
     3781 atgttgaact gctcaggtga gatgaactgc agcttgtggg cgtagagcgc cccggcaatg
     3841 ccggccagcg cggctgacag cgcgaacgac agggttttgt agcgggccag atggatgccc
     3901 atgctttgtg ccgaaatttc cgagtcgcga atcgccacga aggcgcgccc ggtgggcgac
     3961 cgcagcaggt tcagaatggc caacgtggcg ccgatggtaa tcaccaggca aagaaaatag
     4021 aactcttcgc cgctgcccag cttccagccg aacaggtcgg gtgccttgat atggatgccc
     4081 gagttgccgc cggtgacaga ctcccaccgc gcaaacactt cttcgacgat gaaaccgaag
     4141 gacagtgtgg caatgccaag gtagatgccc ttgacgcgca gcgccggcag cccgaccacc
     4201 aggcccaccg tggccgacag gcccgccgca caggccagtg caatcgggaa gggcacgccg
     4261 gcattggtca tgaccgcctg ggtgtaggca cccaccccga gaaaggccgc gtggcccagt
     4321 gaaaacaggc cggtgaatcc ggccagcagc atcaggccca ggccggcgat ggcgtagatc
     4381 aggatgaagg tcagctgtgc cagccagtac tcggcgaaga gccagggcgc ggcgaccagc
     4441 agcaacacca gcgcgctgta ccagaagatg tggccgccgt gcttggcaag attgatgtcc
     4501 tgcccgtaat cggtcttgaa gataaagcgc atgagttatt tttctgattg caatgacaac
     4561 gggcgtgaga tccccaactt caagcagggg ccgcggaacc ggctttgccg ggccgcaggc
     4621 gcagcggccc ccccgggggg cagcgtatta cacgaagtga aaagcgtggg ggtcatacct
     4681 tcttccgcag tttttcgccg aacagcccat tgggtttgac catcagcatg atcagcacca
     4741 ccacgtaggc agcgatgtcc ttgatgccat ccggcgcata gaaaccggcc agcgattcga
     4801 cgatgccgat gatcaggcca ccgacgatcg caccgggcag gctgccgaag ccgccgacca
     4861 ccgccgccgg aaaagccttc aggccgatga agcccatgtt ggcgtgcaca aaggtgatgg
     4921 gcgcgagcag caggccggcg atggcggcca cagcagccgc caggccccag accaggccgt
     4981 tgagccgctt gaccggaatg cccatgtagt aggcagccag ctggttctgt gacgacgcct
     5041 gcatggcaat gcccagcttg ctgtatttga acaatgcaaa cagcagcagg cacaacacgg
     5101 cggtgctgcc gatgacggcc atctgttcga cattgaggat cagggcgccc tgtgcattgg
     5161 catcgccgcc gatctgccag atcatgtcct tgtagggcac gggcagtgtg tgggtttcag
     5221 tgcctatgcc gggaatcatg gtgatcagcc cgcgcgccac gtagccgaca ccgatggtca
     5281 gcatcacaat ggaaaaagcc ggctggccca ggatgggccg tatcaccagc cgctcgagca
     5341 gcacaccgaa gagggccatg ccggcgatgg cgctgaggat ggccagccag aaggggaagc
     5401 ccagcatggt catgcaggcc aggccgccaa aggcgcccag catcatgagt tcgccctggg
     5461 cgaagctcac cgtttcggtg gccttgtaaa tcaggacgaa acccagggcg atcagcccgt
     5521 aaatgcagcc ttgcgcgatg ccgctgatga tcaattgcaa taactgcact tttactccac
     5581 aataaatttt tacaaagcag gcgcttggta aaaataagcc atagccggaa ggcgggacag
     5641 gccgcttttt tttctgactc gaatctgacg ggactgatca tgagctgcat tcaatgaaaa
     5701 aatcaatccg gattggtgcc gggttgggtt tctacggaga cgcatgggaa ccggtgctcg
     5761 caagcatcga acgcggcggg gtccagtaca tcgccagcga ccatcttgcg gagctcactt
     5821 tggccatcct tcaaaaggat ctgcagcgcg acgcttccct cggctacgct agggacgtgg
     5881 tgcccatgct catgcatttg tggcccgcga tggtgcagcg aaaagtgcgc ttcatctgca
     5941 acgccggcgg gctcaatccg gccggcgcgg cagccgctgt tcgcgacgct tttgcagcaa
     6001 aaggatggac cgcgcgcgtc gccgttgtga caggtgatga ggtcatgggg cagctgtccg
     6061 gcctggcacc ggcacacatg tttaatggcg cgcctttcga ttcggtgcgt gagcgcatgg
     6121 tgtttgccaa tgcctatctc ggcgcgcgtc ccatcgtctc cgcattgcag cagggtgccg
     6181 acatcgtgat caccggccgt gtcgccgatg ccgccctgtt cctgggaccg ctggtgcacg
     6241 agttcaactg gaatctcggc gacgcaggag acacggcgac accggccgac ctgaaccggc
     6301 tggcgcaagg cctggcggtc gggcacctgc tcgaatgttc gggccagggc agcggcggca
     6361 acttcggcag cgccgaagcc tggaaagcca ttcccgacct ggcccacatc ggctacccga
     6421 tcgccgaggt cagcgaagac ggcgatgcgg tgatcatgaa ggcgccgggc acgggcgggc
     6481 gcatcaactt cgacacggtg cgtcagcaac tgctgtatga agtgcacgac ccgcaccagt
     6541 atttttcgcc ggatgtggtg ctggacctgt ccacggtccg gcttgaggac ctgggccagg
     6601 accgggtgcg tctgaccggc gcgaccggcc acccgcgcac ggaccagttg aaagtcgtcg
     6661 gcggttaccg cgacggtttc aaggccgagg tgacctgggg cttcagctgg cccgatgcgt
     6721 atgacaaggc gctggcggcc gtcgagatgg tgaaggcgca actggccgac aagggcgtgc
     6781 cgtacgaggc tttgtttgtc gagttcccgg gcttgaattc agcgcacggt gcgctggcgc
     6841 ccctgcccga tgcacaggcg ctggccgggc tcaacgaagt ctgggtgcgc atggtgctgc
     6901 gcacgcgcga caaaaaagcc gccgacggtt tcgggcggct gttcccgtgg atgggactca
     6961 gcgggccggc ttacacctgc ggtttccacg gcctgcacaa caccagcgag ctgctgggca
     7021 tctggccggc cctgatcgat cgcagcctgg tcgagcccaa cacccgcgtg gagctgctgc
     7081 agacatgaca acccccactc aaaaaatcca gcttgtcagg ctggcccacg cacgcagcgg
     7141 cgacaaggcc gactgggtgg acttcggcct gtttgcctgg aacgcggcag gttacgccgt
     7201 gctggcgcgc gaggtcaccg cgcagcgtgt ggcagaacac ttcgcaccct ggctgccggg
     7261 tgaagtcgaa tgctggaccc tgcccaacat cctggcgatc aagctggtgc tgcacgacgc
     7321 gctgcagggc ggcggtgcgc gcaatctgcg gctggacaac ctgggcaagt ccatggcggc
     7381 tgccttgttg cgcatggaga ttgatgtcaa cgccgacgag ctggcggcgt gtctcgccgc
     7441 gccgcgcgtc ggctggtggg gagacacatg acagtccata tagagaagga tggcgaggtg
     7501 accctcgtca ccctggagcg ccccgaggtg cgcaacgcgg tggacgctga acatgcgcgt
     7561 gccttgtatg aggccttcct ggccttcgat gccgacaaca gcgcgaaagt cgcggtgttc
     7621 cacggtgcgc acggacatta ctgcgccggc tgggatttac agtccggcgc gaagatgctg
     7681 aacgaaccac aggacgccgg gccggatatg tttgccgcgc tggattttca ggccgaagac
     7741 agccagccat tgggcccgat gggtccgtcc cgcctgctgc tgtccaaacc ggtgattgcc
     7801 gcggtcagcg gcgcagccgt cgccggcggc atggagctgg cgctgtggtg cgacatgcgg
     7861 gtgatggagg aagacgccta cttcggcgtt tattgccgcc gtttcggcgt gccgctgatt
     7921 gacggcggca cggtgcgcct gccgcgcttg gtcggcatgg gccatgccat ggacctgatt
     7981 ctgaccgggc gcaaggtgga ggcgaaggag gcgctgcaga tgggcttgtg caaccgggtc
     8041 gtgtccaaag gcacggcacg tgaggcggcg ctgttgctgg cgcggcagct ggcggcgttc
     8101 ccccagaaga ccatgctggc cgatcgccag agtgcttata cccagtggga cctgcccatg
     8161 ccgcaggcgc tcaagcagga atgggaccgc ggccgggact gcatcggcga gggcctgcag
     8221 ggcgcggccc gttttgccgg cggccagggg cggcacggca agttttaatg atgaaattgg
     8281 cctccagccc ttgtctgacg ggcggaggca gctatcacaa cgatagcggc cgcggcaacg
     8341 acgacggcgg cacggggcgc ggctggccgg cgtcgtcgat cgccacatag gtcacgctgg
     8401 cctcggtcac cttgatgtag ctcccctgcg agccaaaccg ttcggcatac acctcgacct
     8461 tgaccgtcat cgaggtacgg cctatgcgcg tgagttcgga gtagaaggac aggatgtcgc
     8521 cgacgcgaac cggctgcttg aagatgaatt cgttgaccgc gacggtggcc atgcggccgc
     8581 cggcataacg cgccgggatc accgagccgg ccaggtcgac ctgggccatg acccagccgc
     8641 cgaaaatgtc gccgttggca ttgcagtccg ccggcatcgg aatcaccttg agcaccagtt
     8701 ccttgtcgag aggcagtttg acgttcaggg cttggggggg aaggccggtt tcgttggaca
     8761 taggcacaat cggtattgaa aaattgaaac aacaggacaa ttgtctccga tgcgctccca
     8821 tgcccccgcc gccgaaacgc cgcccgcccc cggcgcacct ccccaacctg cgctgaaccg
     8881 ctccgactgg gcgaccctgc agcgcctgtt cccctacctc tgggactaca aatggcgcgt
     8941 gatggccgcg ctggccttca tggtcggcgc caaactcgcc aacgtcggcg tgccgctgct
     9001 gctgaaaaac ctggtggacg cgatgagctt caagccgggc gatgtgcagg ccgtgctggt
     9061 cgtgccggcc gcgctgctgg tcggttatgg gttgctgcgc ctgtcaacct cgctgtttac
     9121 cgagctgcgc gagctggtgt ttgccaaggc caccgaaggc gcggcgcggc gcatttcgct
     9181 ggaggtgttc cggcatctgc atgcgttgag cctgcgtttt cacctggagc gccagaccgg
     9241 cggcatgaca cgcgacatcg agcgcggcac ccgcggtgtg cattcgctga tttcctattc
     9301 gctgtacagc atcatcccga cactgatcga agtcacgctg gtgctgacgt tgctggcggt
     9361 caaattcgac gtctggtttg cgggcatcac catcatcgcg ctggtgttct acatcagctt
     9421 caccgtgacg gtgaccgaat ggcgtaccaa gttccgcaag gaaatgaacg aactcgactc
     9481 ctcggcgcac agccgcgcca tcgattcgct gctgaactac gaaaccgtca agtacttcaa
     9541 caacgaggag ttcgaggccc tgcgctacga cgaaaacctc aagcgttacc gtcgcgccgc
     9601 cgtcaagagc cagaccacgc tgagcctgct caacaccggc cagcagctga tcattgccac
     9661 cggtctggtg gccatgctct ggcgcgcgac ccagggcgtg gtggacggcc gcatgacgct
     9721 gggcgacctg gtcatggtca acgccttcat gatccagctc tacattccgc tgggctttct
     9781 gggcgtgctg taccgcgaga tcaagcaaag cctgaccgac ctgtccaaga tgttcaacct
     9841 gatggagcgc gagcgcgaga tcgccgacga acccggcgcc ccggcgctga gcctgcaggc
     9901 ctcgccggaa gtacgttttg aaaacgtcag ttttgcctat gagccgtccc ggcccatcct
     9961 gcaccacatc agcttcgtga taccgccggg caagacggtg gctgtggttg gttcgtcagg
    10021 cgcaggcaag tccacgctgg cacgcctgct gtttcgcttc tacgatgtcc agcagggccg
    10081 catcaccatc gccggccagg acatccgcga ggtgacgcag gccagcctgc gccaggcgat
    10141 cggcatcgtg ccgcaggaca ccgtgctgtt caacgacacg gtcgaataca acatcgccta
    10201 tggccagccc ggcgccagcc gcgcccaggt ggaagaggcc gcgcgcgcgg cccgcatcca
    10261 tgacttcatt gcctccactc ccgccggtta cgccaccatg gtcggcgagc gcggcctgaa
    10321 actgtccggc ggcgagaagc agcgcgtggc gattgcccgc accctgctga agaacccgcc
    10381 catcatgatt tttgacgaag ccacctcggc gctggactcg gccaatgaac gtgcgatcca
    10441 ggccgagctg cgcagcatcg ccgaaaacaa gaccacgctg gtggtggcac accgcttgtc
    10501 caccgtggtt gacgcccatg aaatcctggt gatggaagcc ggccagatca tcgagcgcgg
    10561 cacccacgcc gccttgctgg cgctgggcgg gcgatatgcg tctatgtggg cgctacagca
    10621 gagctccgag taatccttga atgtttttgg cctgcagccc aataactgcg ggcgcaagca
    10681 gctattgttt agatagcgaa aacagaggtt tcgttaacgc attgcaaagc gctcagcttg
    10741 ccgtcgccgg ccggttccag aattgctgtt cccgtgggat tccccgtagg cttcggtctg
    10801 ctttcaactc gcataatcga aatcatggaa accaaatggc tggaagattt cgtcagtctc
    10861 gctgaaaccc ggagcttcag ccgctcggcc acgctgcgcc acgtgacgca accggcgttt
    10921 tcacgccgca tccaggcgct cgaggcctgg gcaggtaccg atctggtgga ccggagctcc
    10981 taccccacgc gcctgacccc tgctggcgaa acgctgtaca gccagtcgct ggagatgctg
    11041 cagggcctgc agtccacacg cgccatgctg cgcggccaca actcggccgc gcatgacgtc
    11101 attgaattcg ccgtgcccca ttcgctggcc ttcacctttt tcccggcctg ggtcagcagc
    11161 ctgcgcgaga agttcggccc catcaagagc cggctgattg cgctcaacgt gcatgacgcg
    11221 gtgctgcgcc tggtcgaggg cagctgcgac ctgctgattg cctattacca tgactcgcag
    11281 cctttccagc tcgatgccga ccgttacgag atgatcagcc tggggcagga agtgctggcg
    11341 ccttttgtga aagtccaggc cgacggcacg cccctgttcc gcttgccggg tcgggccggt
    11401 cagcccctgc cttacctggg ttatgccccc ggggcttacc tggggcgcat ggttgatctg
    11461 atcctcaagc agtcgagcac cgccatccac ctggaccggg tgtatgagac cgacatggcc
    11521 gagggcctca aggccatggc gctggaaggc cacggcattg cgtttttgcc tttcagcgcc
    11581 gtcaagaagg agttgcgggc caaaaagctg gtcagtgccg gcgagggact ggaaatgacc
    11641 atggacctgc gcgtctaccg cgagcggccg gccggcaaga ccgctgccaa aagcccggcg
    11701 caggccctgt ggcattacct cgaaagccag gcccagccga agacgtccgg caaaccgcgc
    11761 cttggtagca acccttagtg gttataaata atatgcataa tgctgcaccc aaacagcatt
    11821 ggcttttcac gaaccataaa tttacagtcc gtcacgttct gccaaggcga ttggcgtgct
    11881 tcccggcgag acctgcgaag gtcggtgggt tggcacaaag actgcttgtc aggacatccg
    11941 tgttccatcg aatcaacgca cgcactatct tggggactgc tttttgtttg atccgctgat
    12001 ggctttcaac ctttcggacc tttcggattc ttccccggtg ttgccagcct cctgcgcgtt
    12061 gatcccttta tggaactgtc cagtccaccc gatgccgttt ccagggctgg ggtaaacctt
    12121 ggggttcacc ccggtacccc ctccttagaa tggtattcac caaccttttc atttaacccg
    12181 aaggaaactc tccatgaaac tcaaactggc cgctttatcc cttgccctgc ttgccagcag
    12241 cggcgctttt gcccaggcca ccgacacgct cgccaaagtc aaggcttccg gcgtgatcac
    12301 catgggagtt cgcgattcct cgggcgccct gtcttacacc ctgggtgacg gcaagtacgc
    12361 cggttttcac gttgaaatct gtcagcgtgt cattgccgac gttgaaaaag ccgtgggccg
    12421 caagctcgag atcaagtacc tgcccgtgac ctcgcaaaac cgcatcccgc tggtgcaaaa
    12481 cggcacggtg gacatcgagt gcggctcgac caccaacaac gctacccgcc tgaaagacgt
    12541 gtctttcctg cccaccacct tcgttgaaga agtgcgtatc gccgtgaaag ccaactccgg
    12601 catcacctcg attgcccagc tcaatggcaa aaacgtggcc accaccaccg gcacaacgtc
    12661 cgttcagaca ctgcgcaaga acgagcgcgc caaaggcgtt gacttcaagg aagtgttcgg
    12721 caaggaccac tccgacagct tcctgctgct cgagtccggc cgtgccgacg cctttgtgat
    12781 ggacggcgcc atcctggccg gcaacatcgc cacttccaag aaccctgccg atttcaagat
    12841 cgtcggcgaa gtgctgagcg tggagccgat tgccatcatg atccgcaagg atgacgccgg
    12901 cctcaagaag attggtaccg acaccatcac cggcatgatc aagtccggcg acatggccaa
    12961 agcctgggac aaatggttca tgcagcctat ccccccgaag aacagccgtg tcggcctgcc
    13021 tgtcagcgaa agcaccaagg cagcctgggc cacccccaac gacaagccga tggaagacta
    13081 cgccaagaag taatcctggc ttgagctgaa cctcaaaccc cgtctgtgat cttgctggcg
    13141 gggtttgttg tttggtggca gccttggcga acgcgatgca gcgccggctg ccggtggata
    13201 gattgaaaaa ttcctggcgg gagagaacat gaattgggat tggcaggttt ttctgaatga
    13261 cgacggcagc ggccggacgt atctggaatg gatgttcgaa gcctggggct ggaccctggc
    13321 ggtggccggc agttcctggg tggtggccat gctggccggc ggcctgatgg gcacgctgcg
    13381 caccttgccc aacagcccgt ggctggtgcg gctggccaat gtctgggtcg agctctttcg
    13441 caacgtgcct ttgctggtgc agatcttcat ctggtatttc gtcgttccca agatgttccc
    13501 tttcatgcag caggtaccga gctttttgct ggtcgtgttt gcactgggct tttttacctc
    13561 ggcacggatt gccgagcagg tccgcgccgg cgtgcaggcc ctgccgcgtg gccagcgtta
    13621 tgccggcatg gccatgggtt tcacgacagc ccagacttac cgttacgtga tcctgcccat
    13681 ggcgtttcgc atcatcatcc cgccgctgac cagcgagtcg atgaacctgc tcaagaactc
    13741 ttctgttgcc tttgccgtgt ccattgccga gctgaccatg tttgccatgc aggcccagga
    13801 agaaacctcg cgcggcgtgg aggtctacct ggccgtgaca ctgctttatg ccgtttcagc
    13861 cttcgcggtc aatcgcgtca tggcttttgt cgagaaaaaa gtccagattc cgggctacat
    13921 cgtggccggc agccccgttg gggggcactg acatgttcgg tcttgacatg agcttcctga
    13981 actgggacat catttccaag ttcgtcatca agggtttcat tttcagtgtg gagctgacgg
    14041 tgatcgcgac cataggtggc atcctgctgg gcaccctgct ggccctgatg cgcctgtccg
    14101 gcaccaaggt gctgacgatc cccgccacct tctacgtcaa cggcatgcgt tcggtgccgc
    14161 tggtcatggt gatcctgtgg ttttacctgc tgatgcctgc cgcttttttc gacatgattc
    14221 ccggcggcgc caacaatcgc tccgaaatct cggccatcat cacctttatt gcgtttgaag
    14281 ccgcctattt cagcgagatc atgcgcgccg gcatccagtc gatttcgcgc ggtcaggtca
    14341 acgccgggcg cgcgctgggc atgacctatg cgcagaacat gcgcctgatc gtgctgccgc
    14401 aggccttccg caacatggtg ccgattttgc tgacgcagac catcatcctg ttccaggaca
    14461 cctcgctggt gtacgccatt ggcgcctacg acctgctcaa gggcctgacg acggccggca
    14521 agatctatgg tcgccccgaa gaggcctacc tgctggccgc cgttgtctat tttgtgatct
    14581 gttttggctt gtcgtacgtc gtcaagaagc tgcaaaacaa gatcgccatc attcgctaac
    14641 aacccatcgc acccgctccc cggagaaaca agatgatcga actcaaagaa gtttccaaat
    14701 ggtatggcgc cgtgcaggtg ctcaacaact gcagcaccag catcaagaag ggcgaggtgg
    14761 tcgtggtctg cggcccgtcc ggctcgggca aatcgacgct gatcaagacg atcaacgcac
    14821 tcgagccttt ccagaagggc gagatctacg ttgacggcca ggccgtgcac gaccccaaga
    14881 ccgacctgcc caagctgcgc tcgcgcgtcg gcatggtgtt ccagaacttc gagctgtttc
    14941 cgcacctgag cgtgacggaa aacctgaccc tggcccagat caaggtgctg ggccgcaagc
    15001 ccgacgaagc caaggcccac ggcctgaaat acctggaccg cgtgggcctg atggcgcaca
    15061 aggacaagtt tcccggtcag ctctcgggcg gccagcagca gcgtgtggcg attgcccgtg
    15121 cgctgagcat ggacccgatt gccatgctgt tcgacgagcc gacctcggcg ctcgaccccg
    15181 aaatggtcgg cgaggtgctc gactgcatga tgggcctggc cgtcgagggt atgaccatga
    15241 tggtggtcac gcacgagatg ggttttgccc gcaaggtcgg cagccgcgtg attttcatgg
    15301 acgtgggcgg caagatcctc gaagactgct cgaaggacga atttttcagc catcccgaga
    15361 accgccagga gcggacgcgg gacttcctca acaagatcct gcagcattga gcgctactgc
    15421 gcgggcgcac ggcggcgttg cgggtcccga tcaggccatc ctcacgtacc tgaagtacgt
    15481 tccggtggcc tgacacccgc gccttgccct gctcccgctc gctacgcgcc agagatggcg
    15541 gtgggggcag ggggccggag atttgggaca atgggggcat gactcaaata accatcaccc
    15601 gtcccgacga ctggcatctg cacgtgcgtg acggtgacgc cctgcgcacc gtggtgccgc
    15661 acaccgcggc gcagtttggc cgcgccatca tcatgcccaa cctgcgcccg ccggtcacca
    15721 cggccgcgca ggccatggct taccgcgagc gcatccaggc cgccgtgccc gcaggcgtgg
    15781 ccttcgagcc gctgatgacc ctgtacctga ccgacaacct gccgcccgat gaaatcaaac
    15841 gcgccaggga tgccggggtt gtggcgctca agctgtaccc ggccggtgcc accaccaaca
    15901 gcgacgccgg tgtgaccgac ctgcgcaaga cttacaggac gctggaggcc atgcagcgcg
    15961 aaggcctgct gctgctggtg catggcgagg tgacgtcgcc cgagatcgac ctgttcgacc
    16021 gcgaggcggt gttcatcgat acccagctga tcccgctgcg ccgcgacttt ccggaactga
    16081 aaatcgtgtt cgagcacatc acgacgaagg aagccgcgca ttatgtgcgt gacgccggcc
    16141 gctttctggg ggccacactc acggcgcacc acctgctgta caaccgcaac gcgattttta
    16201 ccggcggcat ccggccgcat tattactgcc tgccggtgct caagcgcgag acccatcgcc
    16261 tggcgctggt cgaagcggcg accggcgaca acccccgctt ctttcttggc accgacagcg
    16321 cgccgcaccc ggcgcatctg aaggaacacg ccaccggctg cgccggctgc tacaccgccc
    16381 acgcggcgat ggagctgtat gccgaagcct ttgatgccgt gggcgccatg gacaagctcg
    16441 aagccttcgc cagcttcaac ggggcggact tctacggcct gccgcgcaac accggcacca
    16501 tcacgctgaa gaaggaaagc tggatgccgc ccgagagttt tgccttcggc gaagctgaac
    16561 tcaagccgct gcgctcgggc gaagcgctgg cctggaaact tgttggcaat gggagcccct
    16621 gatggaaagc aaaccccgcg ttgcgttgct gattgatgcc gacaattcgc cggcctccaa
    16681 gatcgacctg atcctcaacg aactgtcgac cttcggcgag accaacatcc gccgtgccta
    16741 cggcaactgg aaaaaaagcg agctcaaggg ctgggaagaa gcgctgcacg aacacgccat
    16801 ccgtcccatg cagcagttcg attacacgaa gggcaaaaac gccagcgaca tggccatggt
    16861 gatcgacgca ctggagctgc tctacaccga ccggcccgac gctttcggca ttgtctcgtc
    16921 cgacgccgac ttcaccccgc tggtcatgca cctgcgcgcc aagggggcgg cggtctacgg
    16981 ctttggcgcg cagaaaacgc ctgagccctt tgtcaacgcc tgctcgcgtt ttctctacct
    17041 cgacaagctg cgccccataa ccgcggccga tgcgcccgct ggcggccggg aagacgccga
    17101 agccgtggcc actgcgcccc tgcgcgccac accggcgcag ctcaagcagg acgccaaact
    17161 cgtcatgctg ctgcgcaacg ccgtgcaggc cgccgccgac gacgagggct gggcgcgcgt
    17221 cggtgcggtc ggcacgcaaa tcgccaacca ggcttcgttc gaccaccgca actacggcta
    17281 cgccacgctg accaagctgc tggtggccac gcagctgttc gacatcgtcg acgaaggcaa
    17341 gtccacggtg atggtgcgcg acaagaggct gtcgcggccg gctgccagga acggatgagc
    17401 gctgacgcgc cagccgcagc ctggccggtg gactggaatc gcccctggct ggcgccgtgg
    17461 cgcgaacccg gtgaacgcgt ggctcaggcc atcgcagccg gtgccttgtg tcatgaggcc
    17521 ctgaaccgcg agccggcggc gccacggcgt tttgtgccgc agtccgactt gcccgacggg
    17581 caggcctacg agcagttcat ttttgagacc ggcaccgttc cgacacgcga caacctgcac
    17641 gactttttca acgggctgtg ctggatgcgt tttccgctga ccaagaaaaa gctcaacctg
    17701 ctgcaggccg cagaaatcgc cgcggccggt gtggcgcctc tgcgcgggcc ggtgcgtgac
    17761 gcgctgacgg tgttcgacga aaacgcggcc ttcctgtcgg caccgccccc gctgtgggat
    17821 gcgctggtgg atcgggactg gcagcgcctg tttgtcgact tgcgcccgct ctggcagcag
    17881 acgcagctgg tgctgtttgg ccatgccttg ctggagaagc tggtttaccc acgaaaaccg
    17941 atcacagccc atatctaccg ggcgcaacca gctatgaatt ccatagcgga gctggatgcc
    18001 tgggtggccg ccgacctgag cgcggacaag ctcgctgcca aaccgtttgt gcccttgccg
    18061 gtgctcggtg tgccgctctg gtggccggaa aacgagaact tttcctttta tgatgactct
    18121 ttcgtattcc gcccgcgccg gtaaacaaca ccaagtaaaa aatcctgcgc ttgatttggg
    18181 ctgaaccctc cccatttgcc cgcaacagtc cctttttttt gcggcacttg tttttaccga
    18241 cctctacgga gcatccatga agcgtatttt tctatttgtc atgaccaacc tcgccgttgt
    18301 ggtggtgctg ggcattgtcg ccagcctgct gggcgtcaat cgctacctca cgccgcaagg
    18361 gctgaacctg ggcatgctgc tgggctttgc cgcggtcatc ggctttggcg gcgcgatcat
    18421 ctcgctgctg atcagcaagc cggtggccaa gtggagttcc ggtgtgcgcg tgatcaacga
    18481 accgcagaac gccgacgaag cctggatcgt cgagaccgtg cgcaagctgg cgcagacggc
    18541 gcagatcggt atgcccgaag taggtatttt tgaaggtgcg cccaatgcgt ttgccaccgg
    18601 cgctttcaag aattcatcgc tggtggcggt atcgaccggg ct
//
LOCUS       DAIDFG010000019        18526 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_19_length_18526_cov_18.663851, whole genome shotgun sequence.
ACCESSION   DAIDFG010000019 DAIDFG010000000
VERSION     DAIDFG010000019.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 18526)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18526)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18526
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19_length_18526_cov_18.663851"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3030 a         5782 c         6589 g         3125 t
ORIGIN      
        1 ggatcacgtt gatcaggtcg tacaccacgc ggcgctcgca gcccatgatg aggtcgaggt
       61 tgcgcaggcc gacgtcgaag tcgatgacgg cggttttatg cccgcgcagg gccaggccgg
      121 ttgcaaagct ggcgctggtg gtggttttgc ccacgccgcc cttgccggag gtgaccacaa
      181 tgatttttgc catgcgtaaa agtccttaaa gaatgagaag agatggctgt cccgctatcc
      241 gttcgtcctg agcctgtcga aggatggcca ggaacccttc gacaagctca gggcgaacgg
      301 ccaaacctga aaagcggggc ctcatgtttt gatcagatcc atgaccagct tgtcgccttc
      361 gggaccgggc accaggcgca cctgcgtggg ctggccccac acaccatcag gcaacgggtt
      421 ttcgctggtg cggtagatgc cggcgatcga cagcagctcg ggctccaggc tgagcgcaaa
      481 aatgcgcgct tcggtgttgc cgcgcgcgcc ggccatggcc ttgccgcgca gcggtgcgta
      541 gacatggatg tggccgtcgg caatgacctc tgcgccggcg ttgaccatgg ccagcacgac
      601 cagatcgcgc ccgcgggcat acacctgctg gccggagcgc agcggcttgt cgatcaccag
      661 tgcgccggga gccggcgcgg cgtgcgcttc ggccaccggt gccgtcgcct tgctgcgcgt
      721 ggcgggtgcc ggggcggctg cgggagcgct gacaacacga gcgtccggcg cggcgaccag
      781 cccggcggcc agggccgcgg ccagttgcgc cgggttgccg ccgcgcactg ccagaggggc
      841 gacgcggtag ggccgcagca gcgcgatcag cgctgcaaaa tcaaccgtgg ccgcgtcaag
      901 aggcaactgc gagaggtcga tgaccagcgg gtcgttgtcg aaaaaatcag ggatatcgcc
      961 gaagcgcagc gccatctcgc gcgacagggc gctgagatcg gaggacttga ggagcaacgc
     1021 caccaggggc aggttggcgc ttttgatttc gaaggtggcg ggggtgctgg ccgggatggc
     1081 aacggtcatg agggataggc gtcgcgttgt cgcggaaagg agagaaaaaa cgggcgattc
     1141 agacgggtct gaaagggacg aatcttaaca gcggggcatg acgcagttgt gagcgaatga
     1201 aacgcctgtg attgtgtgag gatgcgcctc atgacacagg caagcccatg tcctgcgcac
     1261 gaaacccggc gacgacctgc gcggaggcga tgcaccggca gggctgagcg ggaaaagcat
     1321 tgtctacaag caaaaaaggc ctccagccct tggttggtgg gcgcaggcag ctatgtaaat
     1381 aatagcgtca ctgtgtctgc cgagcggcgg cgttgatatt gcgcgccgtg ccgagcaagg
     1441 tggcgcacag gacttcttcg ccctggctgc tgacggcgta cacgtctgcc gcacagatgg
     1501 tcagcagctt gcccgcgctg accacccgcc ctcgcgcgcg cagcagcgca ccttgcgcgg
     1561 gcgccagcag cttgatggtg gcgtccaccg tggcgttgac ccaacccggt gccagcgagg
     1621 taccggcggc cgccacggcg gcaaagtcgg ccacggcaaa caccgcggtg gcctgcagct
     1681 ggccggggcg aaagcagaag gcctcgagca ccgggatttc gacctccact tcgccgggca
     1741 cgatgcgcct gaaggccagg cccagggtgc gggccatggg catggcgagc accagttgtt
     1801 caagcggctg gaactgcggg gagctgtgtg gggcttcggt cgtcatggtc tggttttcct
     1861 gcgtggtgtg gaaagggcgg cgggccaggg tgccttgccg ttgggtgttg ccgcgaggcg
     1921 ttgcgctttt ttggcctggt gcgcgcgctg ctgtttgtgc atccacgcga tgtgcgcgac
     1981 cacggcgcgg cgctgggttt tgagttcgcc gatctgcgcg tctatttctg ccacgcgggc
     2041 ctgcatggct tcgaccatct gttcaaaact cagccgtccg gcgcggtaag cgggaagctg
     2101 cgccgcaatg gccttgagcg aaaaaccgat gcggcgtgac atggcgatga ataccacctc
     2161 gcggcgcatg gcgtcggtat attcgcggta gccgctggcg cggcgcgcgg gctggagcat
     2221 gccgagcttt tcatagtggc gcagggcgtg cacggaaacg ccggtgcgtt gggcgagttc
     2281 agagatctgc atggccacca cattagcaag cttcgccctg cacgaagcta gtgcagtgtt
     2341 gcacgctatc tggaacataa aagcgcgtct tttgcgccat ggcggcgttg caaatccgcg
     2401 cgataccctg ggtatcgctg tggtttgcgc cttgccctga cccaaaatcc atcgctttta
     2461 ttcgttccat caagcatgca acactgcacc agggcctgtc ctgccgctgt cacctgcgcg
     2521 gcagctggcc tgcggcgcac aactgttaca gccatgcggg ctggctgccg ataagctggg
     2581 gcgtgagcgc gtcccgtgtt gaaactgcgg acctgccact gtgcgggcgg atcaggaaaa
     2641 gggagacggt atgcgcatgc aacactgcac tggaaaccct tgcgccagga caacccgccg
     2701 cgccccgcgg gtgataggct ggcgccttgc ccctgaaaga aggagagttt ccatgaaggc
     2761 ccctttctca agttactcac ggtgttcagc tgctttgttg cctttgtttg ccgccatgtt
     2821 gctggccggc ggcgcggcgc ttgcgcaggt ggcccctccg gcagacccgt ccatgcctgg
     2881 gccgcgcatc aaccgcaacc cggaatttcg ccgcaacctg ccgccggcgc cgcccgtgga
     2941 gccggacccg cgcgactcga ctgtccggcc ggtgcagccg gccgaacagc caccggccga
     3001 tggccgcagc gtggtgctgc cgttgcccgg gccgcaggcg cccgcctctg caccggcgtt
     3061 gccggcctcc ggcgcgccgg ggcgttgacc cgggttttca gggcaaattg gcttgtagcc
     3121 tatatgtggc gcgcgcagtc agctatcaaa taaatagcgt caaataagca gcggctgcaa
     3181 ggcgtccggc gagggggcga actcgtcgag ggacagctga gccggcgcgc ccggcggctg
     3241 cgtggggacg gccagcgtgg cccggggctg cggcacacgt ctggccatgc gtgtggcggg
     3301 ctgcgcggtc aggtggtggt tgcgctggtg cgcggccggg gccagcgggt ggagccggcc
     3361 agcctccggc agcttgcgcc gcggcctgcg cggcagggac ttttgcaggc tgcgacccgg
     3421 caggcgcttg ggcttgaagg tgtgctcgta gcaggcaagc cagcgtgttt caccggcgtc
     3481 gggcaggggc gcgtcggccg tcttgatgcc cgggccgaaa tgcagccggg cgccgtccca
     3541 ctgcgcgctg cgctcgggcg tgaggatggc ccagcgcatc tgcgcgaaac gtcgcgcaaa
     3601 aaacgcggcc atgacctcga tgaggtggtg ctcgggcgcg acccacgcca catgcagggg
     3661 cccgccctcc gggtggttgc gaaagctctc gtcctgcacg ctgtgaaaac gcacaaaggc
     3721 tttcatgctg tgcatctcgt cgcgcacggc ctgcgccatg cgctgggcct cggccaggtc
     3781 ggcgtcgagc gggtcgtggc gcaggccggg ctcatgcacc agacgccaca gcaggcggta
     3841 aagcaagtca aaacgccggg ggtcggcatg caggatggcg ctttcgcaca gggccacaaa
     3901 ctcgggcggc acctggactg cgggcgcatc gccgacggcg gtgtggcttt ccggcggtgc
     3961 ggtcagcgga tcctgcaccg gcaaaccggt gcagtgccag ctgacctgcg cgggcaggat
     4021 ctgttgtgcc agcaggccgc gtgcggcgcg ccggaagcct tccaggtcgc cggggccctg
     4081 cagcgtgacg gtcagcagcg cctgcgggaa gaggtcgttc gtcagggggt cactcatcat
     4141 gcccatgccg tgctccttgg gtggccgggg ttgccacctt gctctgatgg tgcgcgttgt
     4201 gaagggattg gcgctgtagt ccgggaggct gactgcactg caggctgggg cgcgtccggg
     4261 gcgctggaaa agggaaatgc ggcagctaca gcccaacacg gggcggcgct tgcgcctgcg
     4321 cgggcacgac gccctgaatg ggcagcgacg gtcgttttgg cgatttgttg cggtgtatcc
     4381 actgtatcaa tatacagtgg tttggcgatt ttgaccagtc atggacggac cggatttccg
     4441 ccatgtgttg tctttcaggg cgaaaaaccg gggcaacggc gcctaaacac cgttaaccgc
     4501 ctaactggga gattcggccg tggcagcttc ggccggcgtt gcctttggcg ccagtttttt
     4561 ggcccgcact tccatgacca ggtagatcgt cgttgcgagc gccagcggca ccaggtaata
     4621 gatgacccgg taggccacca gggcggccag cagctggtgc gtgggcatgg catgcgagag
     4681 cagggcgaca aacaccgctt ccagcacgcc caggccggcc ggcacgtggg cgatgacgcc
     4741 cgccacggcg gccaccagca acacgccggc caccgccgga aaggcaattt tttgctgcag
     4801 cagcagaaac atcaggccgc tcatgatcag ccagttcgtt gcccccatcg ccacctgcag
     4861 cagcgccatc ctgccggagg ggagttccaa ccgatgaccg cgcagggtga tgctgcgctg
     4921 gcgcgacagg gtgcacagca gcaggtaggc cacggcggtc gccagcagca caaaacccag
     4981 gatctgcaga ccggcgctgc ccattttcca gccgggcggc aaggcgggcg tggcaaaggc
     5041 gaagatcagg ccgcccagga acaggtagcc ggtccagttg gtcagcatgc tgagcgacag
     5101 gacccgggtg atggtcccgg tacccagctt cagccgggaa taaagccggt aacggaaggc
     5161 gacgccgccc accagcgagc cgaggttgag gttgaacaca taactgatga aggtgaccag
     5221 catcaccgtc ggggcgcgca gcttgtggcc ggtgtagtgc cgccccagca ggtcaaagca
     5281 gctgtacagg cccaggctca gggcggccag cgccaccgcg cccagcagca gcgccggcgg
     5341 gtaggcgcgc agcgcggcaa acacctcctg ccagtcgatg gcgcggccct gctcgaccag
     5401 cagccaggcc accagggcaa aaaaaaccag cgtggcgacc cgtttgagcc agggccacca
     5461 gaccttgccg gtgatgccgg ccttcttcgg acggcccagg tcgggcaagg cgggcgccgt
     5521 gttgttcatg agacccggcc ctcgttgaca tggctgattt tcacgtccac gtctgtctgg
     5581 cccagcagtt tttcggccag tgtcaggcgc ggcacgtggg ccggcagcca gccaaaccag
     5641 gccgggtagt ggcgcagcag gtgaaagaca aaaaagctgc gcagactgac ccaggggccg
     5701 gcttcggcca cgtccctggc gtgaatttgc gtgcagctgt cctgcatcag ctgctccagg
     5761 tgctcaccga gatgctggtt gaaggcgtgg tccttgatga tgacgttggc ctccaggttc
     5821 agcgacaggc tcagcgggtc gaggttgctg gagccgacgg tggaccattc gtcgtcggtc
     5881 agcgcgacct tgccgtgcag cggccggtcg tggtattcgt agatcttgac gccggcggcc
     5941 agcaggtggt ggtacagcag gcccgctgcg atcttgacaa tcggcatgtc gggctcgccc
     6001 tgcaggatga ggcgcacgtc cacgccacgc cgcgccgcct tgcgcagctg cctgagcagg
     6061 cggtagcccg gaaagaagta ggcattggcg atgatgacgc ggcggcgcgc accgcggatg
     6121 gcgatgcggt agtggcgctc gatgtcgttt cggtgttcca cgttgtcgcg ggtcacgaag
     6181 atggcatcgg aagggccggt ggccggcggc ggggtgccga caggcgcggc ggcggtggcc
     6241 ggccggcgct tgagaacccg ggccaaccgg ctgcctggcg ggtgctggcc gagcagcagc
     6301 tgggcgcgga caaactggtg aacttcggca accagcggcc cttccatttc aaccgcatag
     6361 tcctgcttgg cttcggcccc gaagtctgcg aggtggtcgg ccgaatagtt gatgccgccg
     6421 acaaaggcgc gctggccgtc gatcaccacg atcttgcggt gcatgcggcg aaacaggttg
     6481 gtgcggtagc cccacagccg ggggctgggg tcgaacacat gcacccgcac gccggcctgc
     6541 gtcagcgcca ggatgaaggc gggcgagagg tccggggagc cgtagccgtc gatggtgatg
     6601 tcgatctgca cgccgcgccc tgcggcggtg agcagggcct cctgcagggc ctggccgacc
     6661 ttgtcttcaa acaggatgaa ggtttccagc agcacctcgt gctcggccgc cgcgatgcat
     6721 tcgaacaccc gcgggaaaaa gccttcgccg ttttccagca aggtgaagcg gttgccggcg
     6781 acccagcgtc cgttcatggg gtggtcccgc gtgcaggccc ctggtgcatg tcagaactcg
     6841 atctcggcag ccagcggtgc gtggtccgac aggtgcgacc agggtttgag cggcagcacg
     6901 acaggcgagt ggccgatcgc gttgcgcacg tagatgcggt ccagcggaag caccggaaag
     6961 cgcgccggga aggtgcgcgc cgccttgccg ttggcattga cgaacacctc gtgcatggcg
     7021 gctccttttt cgagcacggc atgggcgcgg cggcgccagt cgttgaagtc gccggccgcg
     7081 atcaccggtg cgctcgccgg aatatccttg cgcaccaggt cacacagcat gtgcagctgc
     7141 ttgagccggt gcgattcggc caggcccagg tggacgcaga tggcgtggac atgcgtgtcg
     7201 cgcccgggca cttccagcac gcagtgcagc aggccgcgcc gctcggggcc gctgatggac
     7261 acatcgcggt tctcgaagtg gacgatggga aacttcgaca gcacggcatt gccgtgatgg
     7321 ccgttggtgt acaccgcgtt gcggccgtag gcaaactgcg gccagatggt gtcggccagg
     7381 aattcgtagt gcggtgtggc cgggtagttg gcgacgcggc tttcgtgccg ggcatgggtg
     7441 ccggtgactt cctgcaggaa caccacatcg gcgccgaccg tgcgcaccgc ttcgcgcagc
     7501 tcatgcagga tgaacttgcg gttgaagaag gtgaagccct tgtggatatt gaccgtcagg
     7561 accttgatgg aaaaactcag ggcggcggtg gcaatcgcct gctgggtctg cgccagtgca
     7621 tcggcctcat cgggcggcgt ttccaccacc gggatggcgg gcgcgacggg ttcgctgggc
     7681 tgaagaggtg tttcggaaga gggcatggcg cggcaaaagg ggggcaaaac cctattgtcg
     7741 cgcggccgcg cccaaaggct gtaggacagc aagctcattt gccgtctggc gggtcttttg
     7801 cccgaatgtc aagcggctgt aaaggtcgat gtcctacgcc cgtcaggggg gctttccggc
     7861 agatccgcgc cgccctgggc cgtaaatttg cgttatccag ttccggcaag gcgctggcgc
     7921 ctgcagatgc cttcccggcg ccctgcaggc tggcagctac ttcagatggt cacgacctct
     7981 cctgacctgc ctgtttcatc acaaggagtt tgaaatgaac aagttttcca tgcgttccct
     8041 ttctgccgct gcggccctca ccgccacgct ggcttttgcc gcccatgccc aggtgggtgt
     8101 cggcgccgac gtcggtgtgc gcaccggtgt caacaacaac atcagcaacg acgtgcgtgc
     8161 caacgcaggc gttggcgtga atgccagcgc ttcttcgtac gggacggcca cacgcagcga
     8221 cagctcgatg ggcgccaaca ccgacacacg tgccggtgcg caggtctcgg gcagcggcgt
     8281 caagggcacg gtcagtcgca cggccaaccg cgctgaaaac gcggttgaac gcggcgccaa
     8341 tgcgaccgcc cgggttggtg cacgcgccgg caacgcggtg ggcaatgcgg cccgccgtgc
     8401 cggcaacagc atccagaacg ctttgcccgc cggcgcgcgc ggcaatggcc aggtgaatgt
     8461 gcagggctcc ggcgctgccg aggcgaaata agcctgcgct gaccacacgg tccagcgggg
     8521 ccgggccgcc ggggcatgtt tccggtggcc cggccctttt tttgcccgcg tggctggcgt
     8581 ctgcgcgctg tgccgtatgc tgcgtctggt tccggcgtgg cctcacgcag gccccacccc
     8641 ctgccgccca cccactgaaa ggcttgcccc atgccccgcg ccatacttga tgagtcccgc
     8701 atccatccgg ccatccaggg caagatcgcc cgccatgagc aggccatcgt gcaggaagtg
     8761 caggccgccg tggccgccca tgcggtggtg gtggtcggca tggccatgaa cccgtttccg
     8821 cgcaaggcgc gccgcgcgct ggatgcggcg ggggtggcgc accactacct cgaatacggc
     8881 aactatctca acacctggcg tgagcgcaat gccctgaaga tgtggaccgg ctggcccacc
     8941 tttcccatga tttttgtgcg cggcacgctg ataggcggag cggccgacct ggacaagctg
     9001 atcgccagcg gcgaactcaa gacgctggtg ggcggctgaa gcggagttca tgcgggggca
     9061 ccgttaacca catgcgtggg gggctccctc ttcaccagcc ggggccgcgg atctggcttt
     9121 gccagtccgc aggcgcagcg ccccctcggg gggcagagag ctacgcgcag cgagcgaacg
     9181 tgggggcgtt acttccgccg tggccgtgaa agacctttgt cgggctgcga cagggtgccg
     9241 gcccgcaggg ccttgacggc gttggccacc gcgctggcga cattgcgcac ctcggcttgc
     9301 acgggcgcgt cggcgtccag ggcgtcgtgg ctggtggcat aggactcgta gtagccgaca
     9361 taacggtcca gccgtgcctg gctaccggcg tcgatcaggc ccatccagtc gagccagtcc
     9421 gacaggccgc gccgcacccc ctcgatgcct gcaacatcgc cgtgcacgac caggccgtag
     9481 gcacggccgg ccaggtgttg ggggtagtcc cagccctgga gctcgagcgc cttggcctcg
     9541 gcggcttttt tcccgtgggt gcgggtgggg tcggggttgc cgccatcggc acagaccagc
     9601 cggtccatca tgagcttgag cgggcttggt gactggtacc agtacaccgg cgtcaggatg
     9661 atgaccgcat gcgccgcggt ccagcgctcg tagatctcgt tcatccagtc gctggtctgt
     9721 ccctgggcgt ggttggggta acagctgcag ggccagtggc acagcggcat ggcggtggag
     9781 acgcagcctt tgcaggggtg aatgttcagg tggtagctcg atgtcagcag gctgaggtcg
     9841 agcacgtcgg tctggatctg cgcctgtgcg agggtttctt cggcccactg caccatgcga
     9901 aaggttttcg aaatttcgcc ggggcaggtg ccgtcgttgc gtgccgagcc gcagaccagc
     9961 agcacgcgcg agggcgtggc cgggtcggcc caggccgcct gcgcctggtc gatgcgttct
    10021 ttcgtcgcca gccactcgac ggacaggtcg tagtcgggat cggcgtagcc ggggccggcc
    10081 ttgcgggtgc ggggcgcctt gcggccttcc tggtaggcgt cccaggcaat ggcctcgacg
    10141 cgggagatcg cctcggcctc ggcctggaaa gccgggtcca tgaaagattc catgaaacgc
    10201 tcatggaaca cgctgcgcga taaagcggcc ggcgcctggc ctttgcggat gggggtcatg
    10261 acgccagttt aggcaggcag ccggctggaa aagcgaagct tgcacctcgt cctacgcagg
    10321 atggcgatgg cgtctggcga ggcggaaatt gtaagtaaaa agtgcctcca gcccaatacc
    10381 tgtctgcgca agcagctcct gaaaacatag cgcttcaagt acgagccacc agcaaaaagg
    10441 cctgcgccac gcccggaagg gacgcggcgc aggccggggg tcaggctgcg ctcaggcgct
    10501 ggcctgcgaa ggctcagcct ttgtgatgct ggccgtggtg tccgccctgg ccctggcctt
    10561 tgccgccgcg gcccatgcgc tggctgtcaa agactttttg ctgctcgggg ctgagcgcgg
    10621 cataaaaggt tttggtcgcc tcgccacgtt tgtccatggc ggcgttcatc tcggtcatgc
    10681 gctgggcgcg cagggcacgc atcttgtcga tgcgctcggg cgtggtcagt ttgtcgaact
    10741 cggccctcat ggcagcatgg tcgggacggg cgccatgaac cggcggttgc atggttgcgg
    10801 tgaaggtcgt ccacgcgcct tcctgggccg gcgtgagttt gagtttggcc ttaaggtcgg
    10861 cctggtgcct ggcgatgcgc gcctgcatct gggccgggtc gtgacggccc atgcggtcgc
    10921 catggtagcc gccggctttg gccggagcgc ctgctgtggc gggtgccgct ggcgcgacgg
    10981 tttgtgccgt ggcggccagg gccgcggtgg ccatgatgcc ggcaagaacc aggctttgga
    11041 cagatttcag gggtttgaac atggaggtca tgagagcttc ctttcggtag aagacaagcc
    11101 cggtgccggg agtggcgtca ggttgaaagg aagtttgcgc cgcctgtgta agggcgccat
    11161 gaggcgcatg tcaggatttc gtaaagtttt ttgtcgctgc gcggcccgcc ggggtagccg
    11221 gggggaggag ggtgcgctgc tgcaccgggg cagcgggctt ttccggcccg ttgtacaccc
    11281 gcccggttca gttcatggcg agtgcccggg tgccggtgcc tgccaggccg cctcgtccag
    11341 caccctggac agactggcgc gctgcttgcg cggcacgccg cgcaggccgg ccagcaccca
    11401 ctgcgcgcgc gctgtgttga tctggcctgt gtcccgccag cggctggctt ccagtgtcgg
    11461 gttgtgcagc atggcggcca gccagatggc ctgcgcgggt tcgagcgcat gtgcgcgtcg
    11521 gccgaagtag tgctgcgtcg ccgcctcggc gccgcacagg ccgggtcccc agggcgcatg
    11581 cgccaggtag agctgcagca tgcgcggctt gcccagggtc tgctccattt ccaccgcata
    11641 aagcaactcg cgcagcttgc gcacggggct gcgctcgtcg ccggtgacca gcagtttggc
    11701 cagctgctgg ggcagggtgc tggcgccgcg gacgatacgg ccttcgcgct ggttgcggct
    11761 tagcgcggcg ctgaactcgg ccaggtcgta accttggtgt tcgtaaaagc gctggtcctc
    11821 ggcggccacc acggcgcgcg ccagccagct gtcagcggtg aggcggctgg tgcgccggct
    11881 gcagcccgag cgggccgccg caaacacttc cgtgcccaga ccgctgacct ggaaaccttc
    11941 gacttgcggc gccaggctca ggcggccgcc gggcaggctc agctgggcct tcagggcaaa
    12001 ccggccttcg atgcggacgc gcgtcagctc gggaatggcg gcgccgaaca gggcataagc
    12061 gtcggcgatg gcgctgggcg ggagctgcag gtggatctgc agttcgcggt tgctcagttc
    12121 accctgccag gtggcgcgta atgcgcccga ggccagctcg ccgtcaagca acaagccttg
    12181 gcggcggacg ctgagccgca gttcctgaag gacgacccgc ttttcgccca gcccggccgg
    12241 cgaaaccgtg cacggcgcgc aataaagcag cagcgtgcgg gtgggcgcct gccaggtcag
    12301 ttgcaccggc ccggcgcgtg ttgccaagcg gcggccgtcc agcaggggcc cgacccaggg
    12361 cgcggtggcg aggcgcagca tggtcggcac gccggcctgc agctgcagcg ggccggcgcg
    12421 cagcggcacg gaccattcgc cgggcagcgg tgtcagcacc atttttgcta tgaaatagat
    12481 agctgtcagt gcagacacca taagggccaa cagcccaaaa agcatgaaga ccacgaagcg
    12541 cagcgttttc acagcgggct cgcgacggtg atggcttgcc acgggccctg cgactggccg
    12601 agggcggcgg cccagtacca ggccgaggtg tcagtgaagc cccggccgct ggcatccaca
    12661 atgcgctcgc agacccgcag gcgctgcccg ccgcgccgcg cttcaaagca gcgccagagc
    12721 agccgctcgc tgtccagttc gagctgctgt gccgcgatct ggtagcccag accctggtag
    12781 cagtcagtgg ccggatgcag catgcgggtg ggcttgtcca cacttcgcac caccagcacc
    12841 tgcgtgccgt tggtcaggcg gctcagcgtg cccggaaagc gccgtgcaaa acgctgctcg
    12901 acctcgctca gagccagcgg gcgcagcgcc tggccctgcc attgcgtggc gaactcgctg
    12961 ctgccagcgg gctgatgcgg cggggcagct ggccgggtca gcgcagcgaa ggcggcccac
    13021 agcatgcatg cgggcagcag cacggcccac agggttttgt ggatgacgcg gttgatgaaa
    13081 ctgttttcaa acatggcgtg ttttctcggg gcgcgacatc accccggcaa tggcggtaca
    13141 cacggcggcc aacaccagca ggcccacgcc ttcatggacc catccgggca cagggctgcc
    13201 cgaggcttcg gcggccacca gcacggtgtt gcgcacgatg ttgccggcca agaccagcac
    13261 gctgaccgcg ggcaggcggg tgaggaaggc gcgattgccg cgcgccaggt aaagcgtcac
    13321 ggcgcaggcg ctgaagtagc cgaaccaggc catctgtacg cccgaacagg gcgcgtccac
    13381 gatcaccagc cggccgtcca ccagcaggct ggcgccgctg cgttcgacct catggccgag
    13441 catcagcagc cagcggctgg cctcggcggt aatgacgcgc agcgggtagc cggcataaaa
    13501 ctgcagtgag gccagcagcg gcagcgacag caccgccagg cccagcacag gcgcactggc
    13561 caccttgcca ggcaggaagg cgcacaggcc ggcggccagc gcggtgagcg ccagcagcgc
    13621 caccaccagc gagggcagca acccctggct gagcgtggcg gcgattgcgc cggccagcgc
    13681 cagcgccagc cacaccaggc gcggcgcggg ccgcaggcgg cggcgctgtt gccaggccag
    13741 cagcccgagc gccgacaggg ccagcaggcc cagcggatcg tcggagtggt ccagcatgcg
    13801 ctggcccatc cacagccagg tcggccacag cgcggcggcc agcagggcca gccagatcca
    13861 ttcgggggcg gtgtcgatgc gcacgcccca gcgcacaaag ccgggccgct tgagcaggcc
    13921 ctgcgggcgg gccagcgtgt gaaagccggt aaggaaagcc atggctgctg accttcaggc
    13981 ggtgaaacgg gcggcgttgt ggcggcgctg gcggcgtgcc agcatggcca gcaccgacag
    14041 catcatcgcc atgccggccc aggtggctgg ctccggcgtg ccgggcactt cggcaccgat
    14101 ggccaggttg ctcacgccct gcggcatggg cgagggctgg ttcacgctgg gcgtatccag
    14161 cggctgcgtg ttgcgtacga ccttgtccac cgcgaggaag ctggtgtatt gggtcagcaa
    14221 gttgtattgc aggccgagtt cggtgatgcg cgcgcgctgg gagtcgccgc cttcaagcgc
    14281 ttcctggtcg gacaggccgg caacgcggtg ccgcgcccag aggtagcgca gtgcggccgc
    14341 gtcctggttg ctgctgccga ctggcagggc ctggcgatag gcgccgctgg cgctctggcc
    14401 ttcgatgaca agctggccgc cggcggggcc gcgccatttg ccgaagacca ccaccgggcg
    14461 ttcgcccagc acgtcgggca gctgggccgg ctccacgtcg tacacgtcca tgccttcgaa
    14521 acgcgccttc acgctggtca gaacgggcga ctcgatcatc ttgcgaaagc gcgctgcctc
    14581 ggctggcgct tctgcggcgt tggtgatgat gaaaggctcg cccatgccgg cgcgggccag
    14641 gccttccatc aggtggcgat tgaccgagga gccgatgccg aaggagaaca ggttggcttg
    14701 cgagagattg cgccgcacca gctcgaaagc ttcacgctcc accgtgacgt agccgtcggt
    14761 caccaccacg atggtgcgcg acacgtcggc ggtcttcggc tcggcgtaga cgcgcttcag
    14821 ggccgggatc agctcggtgc tgccgccacc gcccatctgc acgatggtgg cgatggcctg
    14881 ctcgatgttg gcgcggctgg cgggcacgga cgtcgggctc aggaagcggt tgctgccggc
    14941 gaacagcagc acgttgaagc tgtcgctggg gcgcaggctg ccgatcagct cgcgcagcag
    15001 ggccttggag gtttccagcg gaaagccgtg catggagcca gagatgtcga ccacaaagat
    15061 gtactcgcgc ggattgatgg tttgcgccgc gatctgcttg ggcggctcga ccatggcgag
    15121 gaagaagttt tcgccgctgc cggggttgcc ctggtagagc atcacgccgg actcaatgcg
    15181 gtcaccggcg aggcggtagt cgaggatgaa gtcgcggttg ttggccgggc cgctgccggg
    15241 gcgcagtgac acgctggcgg ttttactgcc ttcgtcgccg gcttcccggc ttttttgcac
    15301 gtcgatgttg tgggagttgg agcggacttc cttcaggccg ataggtgtgg cgagctgcac
    15361 cttgatgtcg aaagccgtgg ccgaaggctg gccggccggc aggtagggct tggccaccca
    15421 cttcgcctga gactgctctg actgcgggct gttgtagcgc gggccgacca cggtgggaaa
    15481 gacgaactgg tagttgcctg attgcggcac cagcagttcg gtgtagcgca actccacctt
    15541 gacgtcgtcg ccgggcagga tgttggcgac attcatctgg aacacattgg gcaggtgctg
    15601 ctcgagcagg gcggcggtct ttccctcttt tttggcgctg tcatattcga tccgcgcctg
    15661 ctgcttttcg cggatgttgg cggtgatgag tcggtcggcc aggcgcacgt tcatgccgta
    15721 gacggcggct ttggtggagc cggggaaaac gtatttcgcc tcaatcgcac gctggccttc
    15781 gttgcggtag tgctgggtaa cggtgacgtc ggcaatcacg ccggagatgc gcacatcgac
    15841 tttggtcgcc ttgagcggca gctggtccag gccgggctca tcgcttttga cgaagaaata
    15901 ggggctttcg gttttctgcc gcggggcggt gttgtcccgg ccctgggcgt gcgcggggcg
    15961 cagcgtcagc gtgacgaaac aggcggcggc aacaccggcg gtggcaagcc acagccagcg
    16021 cgcggcgggg gccggatgag acagggtgag cggcatgaaa aactccttgg cgggcgtgcg
    16081 acagtgcacg ggtccaagga tggcgaccgt ccgggaaggc ggtttgaagc tttcaggaag
    16141 cttgtgtgaa gtcggcgtga agtgctgtta cgaagcggcg cgcggcagcg acagggtggc
    16201 gacagcgccc tgcccgggct caccgggcac gttggccagc tcgatacgcc cgtcgtgcag
    16261 cgaggcgatc tcgcgcacaa aggccaggcc caggccggtg cttttcttgc gtgaatgcgg
    16321 ccgcgccagc gaataaaact tttcgaatac cttgtcctgg gcgtattcgg gaatgccggg
    16381 gccgctgtcc tgcacccgga tgcgcaccgt gcgcgcctgc agctgcagcg acagcaccac
    16441 ggtgccgccg tcgggcgaga aatcgatggc gttgtccagc aggttgctga cggcgcggcg
    16501 cagcaaaaag gcatcgccct ccaccaccgc gtcgtccggc gcatcgacgg tgatgcggat
    16561 ggcgcgcgcg gccgcggcgt cctgcgctgc gcccgcgagt tcctcgagca gcggcttcat
    16621 ggccaccggc accgggtgat ccagcgcgcg gcgtgtctcc agcgcggtga gctccatcat
    16681 gcggtccacc atctcctgga tgcggccggt ctcgcgcatg atgttggcca aaaagcgctg
    16741 ccgctcggcc tgcggcatgc cggcttcctg cagcagctcg gccgcgccac ggatcgcgga
    16801 cagcgggctt ttcacttcgt gcgtgagggt ctgcacatag tcggccacgt agttgcggcc
    16861 agccagcgcg tcgcgcatct cgtcgtaggc cgcgccgatg gcgcccagcg cgcgccggcc
    16921 caggcgcggc aggttcagcg agcgctgggc ccgcacataa cgcacatagt ctgcgatcac
    16981 gccgaagggg cgcaccagcc agaccgagac gatgacagcc aacagcaaca ccgccagcac
    17041 ggacaccagg ccgacaaaca gggttttctg acgcgcggcg gagacgaact ggccgaagct
    17101 ggtcaccggc ttgccgaccg tggcgacgcc aatgatctcg ttgttccagc ggatgggcgc
    17161 gcccacgtac atcaccgaac tcagcggatc ggcttcgaca tcacgcgtgg tgcgcgcgcc
    17221 gtagtcgccg cgcaaggtgt agctgacgtc tttccactgg gagtaatcgg cgccggcaga
    17281 cttacccagc gagtcgaaca acacacggcc ggagcggtcg gtgacgtagg cgcgcatttc
    17341 gatccgggtt ttggtgaccg aatagatctg cgataggaac tggcgggcgt acagtgattt
    17401 gaacagcggc tccaggcgcg tcgtgtcgat ggcgccggcc tgcacatcct gctcgatcag
    17461 gccggccagc agatgcgagg tttcgaccag tgactcctcg gcggactcgc ggtaacgtgg
    17521 atcaatctcg gcgacgacgc ggtacaggat gaaggcgatg ccggctgcat aaatcagcag
    17581 gatgccgatg aagatgcggt tgcgtttggt cacgacggcg gcaggtcttc ggccagcgcg
    17641 taacccgagc cgcgtgaggt gcggatcggc tcggtctgcg gtgccacggc cttgagcttg
    17701 gcgcgcagcg tcttgatgtg tgcgtccacc gtgcggtcca ggctgccgct gccgtcttcc
    17761 cagacctggc ccagcagttc gtcgcgcgtg aagacgcggc cgggcttgcg caccagcagg
    17821 cgcaggatgc cgtattcata acgtgacagc tcgagcgttc ggccgtagaa gcggatctgg
    17881 cggcgctctt cgtcgagcat gaacacggac tgtgtggtcg ccggggcaga ctcctgcgga
    17941 ctggcagaag gcgtacggac tgtccggcgc aggatgccgc gcacgcgggc caccagttcc
    18001 cgcggcgaaa acggcttggc gatgtagtcg tcggcaccga gttcgaggcc gacgacgcgg
    18061 tcgatctcgt cgctgcgcgc cgtcaggaag agcatgggca cgctggcgcc gccgggcagg
    18121 gcgtgcaggc gcttgaacag ttcaaacccg ttgaggtcgg gcaggccgac gtcgaggatg
    18181 gcgagtgccg gcggctcggc cgcaaactcc tgcagggcct gctgggcggt ggcgcaccag
    18241 accggcgcga agccgtctgt ggccagcaca tactggatcg tgtcggcaat gccggactcg
    18301 tcttcgatca ccaggatgcg gggcttgtgg agcatggggc gtatggtagc tgttggccgg
    18361 gtttgtcatc ccgggctgga tcccgggtcc atgttgcgcg ctacttcgcc tcttccgttc
    18421 gggctgagct tgtcgaagcc ttgttcgatt cccgcggttg tagttcgccg gcaagccggg
    18481 ggttgcccgg cggcaactca ctttcttttt gcgtcgccaa aaagaa
//
LOCUS       DAIDFG010000020        18498 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_20_length_18498_cov_18.596920, whole genome shotgun sequence.
ACCESSION   DAIDFG010000020 DAIDFG010000000
VERSION     DAIDFG010000020.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 18498)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18498)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18498
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20_length_18498_cov_18.596920"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3422 a         5992 c         5975 g         3109 t
ORIGIN      
        1 gaagccatga tctggcgcgg cccgatggcg acgcaggcgc tggagcagct gctgcgccag
       61 accaactgga aagacctgga ctacctgatc gtcgacatgc cgcccggcac cggcgacatc
      121 cagctcacct tgtcccagcg cgtgcccatg accggtgcgg tgatcgtcac cacgccgcag
      181 gacatcgctt tgctcgacgc caaaaaaggc atcaagatgt tcgagaaggt cggcgtgccg
      241 attctgggca tcgtcgaaaa catggcggtc cacgtgtgca gcaagtgcgg ccacaccgag
      301 cacatctttg gcgaagacgg tggcaagaag atggcggccg actacggcat gagttacctt
      361 ggcgcgctgc cgctggacat gcagatccgg ctgcaggccg acaatggccg gcccaccgtg
      421 gtggccgacc cggacggcga agtcgccggc atctacaagg ccgtggcgcg ccaggtggcc
      481 atcacgattg ccgccaaggc caaggatttt tccgccaagt tcccgaccat cacgatctcg
      541 aaaaacacct gatcttcccg ggcgtcgggg gccaaccatg aacatgctcg cttcgctgcg
      601 ttatctggtg gccctcagcg agcaccggca ttacgcgcgg gccgcccagt cctgcggcat
      661 cacccagccg gcgctgtcga acgccctgcg tgcgctggaa aaagagtttg gcatcgccat
      721 cgtcaagcgc ggccgcacct acgccggcct cacgccggaa ggcgaacgcg tactggccac
      781 ggcgcaacgc atgctgcacg aacacgaact gctgcagcaa gagctgcgca gcagtgccag
      841 tcagccgcag ggtgcgctgc agctgggcgt ggtaccgacg gccgtgccgg tggcaacgcg
      901 ttttgcggcc cggctccggc agcgttaccc gggcatcgcg cccgtggtgc ggtcgctgag
      961 ctctcaggaa attgaactcg gcctggagaa cctgtcgctg gacctgggcc tgggatttgt
     1021 cgaacgcctg aaccagcgcg ccagccgttt cgagctgttg ccgcagtaca ccgaacacta
     1081 cttcctggtg cgccgagcgg cgacgcccgg ccggccaggc agcgggctgc gtgtgggccc
     1141 ggccatgggc tggcgcgaag cggcgcagtt gccgctgtgc ctgctgacgc ccgagatgca
     1201 caaccgcagc atcgtggaca gcgcttttgc cggcctgggg ctggcggtca ggccggtcat
     1261 ggaaaccaac tccatcctgg cactggccct cagcgtggtc gccggcgagg tctgcagcgt
     1321 gttgccgggt gcgctggtgg gcgcggtccg cagttatgcc gaactcgaag ccctgccgct
     1381 ggtccagccc gaactcacga cacccgtcgg ctttttcacg ctggccaagg ccagcccctc
     1441 gcgggcgctg caggccgcgc tggtgctggc ccaagagggg gagtggctgc gtcacgcggc
     1501 gcagcacagc gggacgctgc attcagatcc tgaatcagcg catacatgaa ttcaatttga
     1561 cgccggcggg cccaggagcg acagtgcgtc atgaacgttt ccccggtcct tcctgttcct
     1621 gttcccgccg tggccctggc cacgctggac gacatgcgcg agcgcatccg ccgcaaaagc
     1681 aaactcaagg gccgccaggc cgacgaggtt tctctggccg aggttcgcgc gctcataggc
     1741 gggcggcccg aaggcggcca ccggcgtgac ctgctgatcg aacacctgca ccaactcaac
     1801 gacgcctggc gctgcctgca tgagcggcac ctggtggcgc tggcccggga gatgaacatt
     1861 cccatggccg aggtgtacga ggtggcgagc ttttatcacc acttcgagat cgtcaagggt
     1921 gacggccagg cgcccgggct cacggttcgc gtgtgcgatg ggctggcctg cgagctggcc
     1981 ggtgcacagg acctgctggc gcgcctgccc gcgctgctgg ggcaggaggt gcgtgtgatt
     2041 gccgcgccct gcctgggccg atgtgaacag gcgccggccg tgttggtgca ccagcaggcg
     2101 cttccgctgg ccacggccga ggctgtggtc ttgatggcaa atcagccccc agcccagaag
     2161 gggaaagcgc aagaagctat taattttgta gcgtccgagc atgctgaaaa aagtgtcccc
     2221 ggctcggccg acgaggtgct gccggccttc acggactacg ccacctaccg ctcccacggg
     2281 gggtacgcgc tcgcggcggc gctggtcaac ggggaagaag atgccggggc ggtgctggcc
     2341 gccatggaaa attccggctt gcgcggcctg ggtggcgcgg ggttcccggc ggggcgcaag
     2401 tggcgcatcg tgcagggcca gcccggcccg cgcatgatgg cggtcaacat cgacgagggc
     2461 gagcccggca cgttcaagga ccgcacctgg ctggagcgtg atccgcaccg ttttctggaa
     2521 ggcgtgctga tcgcggcgca ggtggtgggc atagcgtcct gctacatcta tttgcgcgac
     2581 gagtaccacg attgccgcac catgctgcag gccgagctgg ccaaactgct ggccgatccg
     2641 ccgtgtccgc tgcccctgat cgagctgcgg cgcggtgcgg gcgcctatat ctgcggtgaa
     2701 gagtcggcca tgatcgaaag catcgaaggc aaacgcggcg agccgcgcct gcgcccgccc
     2761 tacatcgccg aggtcgggct gttcggccgg cccacgctgg agcacaactt cgaaaccctg
     2821 tactgggtgc gcgacattgt ggaaaaaggc ccgcagtggt tcagcggctt cggccgccgc
     2881 ggccgcaagg gcctgcgctc tttcagtgtc agcgggcgcg tgaagaaccc gggcgtcaag
     2941 ctggcgccgg cgggcatcac ggtgcaggaa ctgatcgacg aatactgtgg cggcatggcc
     3001 gacggccacc agctgtatgc ctatctgcca ggcggcgcct cgggcggcat cctgccggcc
     3061 agtctcaatg ggctcccact ggacttcgac accttgcagc cttacggatg tttcatcggc
     3121 tccgccgcgg tcatcgtgct ggggcaccac gaccgggcgc gcgacgccgc gctcaatgtg
     3181 atgcgttttt tcgccgagga aagctgcggc cagtgcacgc cctgccgggt cggcaccgtc
     3241 aaggccgcgg ccctgatgca ggcgccgcgc tgggacgcgc agaccctgga cgacctggcc
     3301 caggtcatgg gtgatgcctc gatctgcggg ctgggccagg ccgcgcccaa cccgattcgc
     3361 agcattcaca agtattttgc gcacgaagtc ggcgaagccc cctggccggg cgacctaccg
     3421 cggccgggtg ccgcggtcgg aaccgagcgt gccgccggca gggaggcttc atgaacgcga
     3481 ccgcccgcct ggccgaagtt ccggcgcagc ccatcagctt cacgctggac ggaaaaaccg
     3541 tgcaggcgca gcagggcgac accatttttc aggccgcgca gcgtctcggc gtggacattc
     3601 cgcacctttg ctaccgcgaa ggtcatcggg ccgacggcaa ctgccgtgcc tgtgtggtcg
     3661 agatcgaggg cgaacgcacc ctggcgccca gttgctgccg ccaggtcagc gccggcatgc
     3721 aggtgcaggc gggcagcgag cgtgcacgca aaagccaaaa gctggtgctg gagatgctgc
     3781 tctccgacat gcccgacacc ggctacaaat gggatgagac cgatgaatcg ctgcaacacg
     3841 gcgagctgag cgactgggcc gatcgcctgg acgtgacggt gcgccccgca ctcaaggcgt
     3901 tgcgccgcgt ccagcccgcc gccgatgtgt cgcacccggc aatggccgtg aacctggacg
     3961 cctgcatcca gtgcaagcgt tgcgtgaccg cctgccgtga ggagcaggta aatgacgtga
     4021 tcgcttatgc gcggcgcggc gcgcagagcg aaatcgcctt tgacctgggc gaccccatgg
     4081 gcgacagcag ctgcgtggcc tgcggcgaat gtgtgcaggc ctgcccgacc ggcgccctga
     4141 tgcccaagac cctggtgggg tcccaggccg tcgataaaaa agttgattcg gtctgcccct
     4201 tctgcggcgt cggctgcctg gtgacctaca aggtgaaaga caacaaaatt gtcagcgttg
     4261 atggtcgcga cggtccggcc aaccagtcgc ggctgtgcgt caaggggcgt tttggtttcg
     4321 actatgcgca cagcccccag cgcctgacca aacccctgat ccgcaaaacc ggtgtcccca
     4381 aggacccggc cgccctgcag catctgaacc gccacacggc ggactggtcg gaggtgtttc
     4441 gcgaagcgag ctgggaagag gcgctgagcc tggcggccgg cgggttcagg ggcctgcgcg
     4501 atggttatgg cagcaagtcg ctggccggtt ttggctctgc caagggcagc aacgaagagg
     4561 cttacctgtt ccagaagctg gtgcgcaccg gctttggctc caacaacgtg gaccactgca
     4621 cgcggctgtg ccatgcctcc agcgtggcgg ccctgctgga gggcgtgggc tcggcggcgg
     4681 tgagcaacca ggtcaacgac gttgcgcatt cggacctgat ttttgtgatc ggctccaacc
     4741 cctcggccaa ccatcccgtg gccgccacct ggatgaagaa cgccgcgcag aagggcgcca
     4801 gaatcgtgct ggccgatccg cgtgtcaccg atatcggcag gtacgcctgg cgcacgctgc
     4861 agttcaaggc cgacaccgat gtggccatgc tcaacgcact gatccacacg gtgatcgagg
     4921 agggcctggt ggaccaggac ttcatcgaca ggcgcgccag caattacgaa gcgctgcggg
     4981 aaaacgtcaa gggctacagc cctgaggcca tggcgccggt ttgcggaatt cccgccgcga
     5041 ccctgcgcga ggtcgcccgg gcatttgccg gggcgaaagc ggcgatgatt ctgtggggca
     5101 tgggcatcag ccagcatgtc cacggcactg acaacgcgcg ttgcctgatc gccctggcca
     5161 ccgtgaccgg gcagatcggc aagcccggct ccggcctgca cccgttgcgc ggccagaaca
     5221 acgtgcaggg cgccagtgac gccggcctga tccccatgat gtttcccaac taccagcgtg
     5281 tggacaacgc ggccgcgcac caatggttcg agaatttctg gaacacgaaa ctcgacgaga
     5341 aacccggtta caccgtggtt gaaatcatgc acaaggcgct ggcgcccgag agtgacccgc
     5401 acaaggtgcg cggcatgtac atcatgggcg aaaacccggc catgagcgac cccgacctga
     5461 accacgcgcg ccatgcgctg gccagcctgc agcatctggt ggtgcaggac attttcatga
     5521 ccgagaccgc ctggctggcc gatgtggtgt taccggccac tgcctggcct gaaaagaccg
     5581 gcaccgtcac caacaccgac cgcatggtgc agatgggcag gcaggcggtc gaggcgcccg
     5641 gtgatgcaag gcccgatctg tggatcatcc ggcaactcgc cagccgcatg gggctgaact
     5701 ggaattacgc aggtgcgcac gatggcgtgg ccgcagtcta cgaggagatg cgtcaggcca
     5761 tgcatgccgc gatcgcgggc atcagctggg agcggctgca gcgcgagtcc agcgtgacct
     5821 atccctgcct gagcgaagac gaccccggtg cgcccaccgt gttcatcgac cactttgcca
     5881 ccgacgacgg acgtgtgcat ctggtcccgg ccgacatcat tcctgccaac gaacggcccg
     5941 acgccgacta cccgttcgtg ctcatcacgg ggcgtcagct cgagcactgg cacaccggca
     6001 gcatgacgcg gcgaactgcc gtgctcgatg ccatcgaacc cctagccacg gcctcgctgt
     6061 gcggtgccga cctgctggcg ctggggctgg caccgggcga tgtgataacc gtgcaatcgc
     6121 ggcgcggggc ggtcgccctt catgtgcggc gcgacgacgg catgccgcag ggcgcggtgt
     6181 tcattccgtt tgcctatcac gaagccgcgg ccaacctgat gaccaatgct gcgctggacc
     6241 cgtacggaaa aattccggag ttcaagtact gcgccgtggc gattcaccgc ggtggccagc
     6301 ccgcaggagc ggcgggttac gggcccggtg ctgccggacg cctttaacct tcaagaccgt
     6361 gggtggcaaa agagttgtgc cgaaaatgcg catgaagaca gccgtacact gcgggcatgt
     6421 ctgaatttgt cctgcagctt cccggtgccg atacggtcat tcgccgtccc tgtgtggcgg
     6481 tggccgtggt catgcgccgt gagcgcatcg acaaccgctg gcagccctgg cgctggtcgc
     6541 tggccgacat cgtgccgcac gaggaaagtt ttggcacgca gccgcgcctg ctgctcaagg
     6601 acgagcagga agagcgctgg ctgcatcccg ggttcaaggt ggagctgttc accgacgacg
     6661 ccgaaggtta ttacctcaac gtcagcacgc cccaaccctg cttctgggtg gtctggcgca
     6721 tggaggaaga ggcggcgctg gccgacgagc cggtggccgt gccgcagacc gtgaccctga
     6781 gttaccacga cgccggtcgc tggctggacg cgcaggagac cgtggagcag gtggcggcgc
     6841 cgcgcgatgt ggtcgagtgg atgcaggctt ttgtcgatgc ccactacgtg ctggaggcca
     6901 agcgtcgcca gcgcccgcaa agcttcaagc cgctgcagga ccggtttggc aaccccgcgc
     6961 gcgtgagcac cgacaagaag tttggcgggg gcggccatgg ctgacgaaac ccctggattc
     7021 ctgggccggt gggcgcggcg caagacggac gcgctacagg gcaaacggct ggacgaaccg
     7081 gcgcctgccg ccaccgcgcc tgtggttcca gatgtcgccc ggacggcagc gccaccccca
     7141 ccccctgcag cacccgtgtc ggccccggac gatgccgtcg cgccacccga aaaaaagctg
     7201 ctgacgctgg aggacgcgca gttgctgacc agggattccg acttcaagcc cttcatggcc
     7261 ggtgatgtcg ggcccgaggt gcgcaatgcc gcgatgaaga agctgttcgc cgacccgcac
     7321 ttcaatgtga tggacggcct ggacatctac atcgacgact attccaggtc cgatcccatt
     7381 cccgagtcca tgctccggca gatgacgagt gccaagtttc tcaatctgtt tgaggatgaa
     7441 gaaaaggaag ggaaagaggg cgttaagacg gcaccggcca cggcaccacg ggaaactgcg
     7501 aataacccca cggatgaaac cgtggcacag tcccacgaag gcccggacct atccgatccg
     7561 gccatttccc cagaacattc cagccaaccc gagccgcagc ctggctctgg cgccagccaa
     7621 gaaaaccatg cccacactga tttgcgactg caaccagacc atgccgctcc agccccagac
     7681 gctgggcgcg gcacttgatg aaaaactgac cctgcattcg gcgctgtgcc gccgcgaagc
     7741 gggcgccttc cagaaggcgg tccagtccgg cgaagacgtg gtggtggcct gcacccagga
     7801 gacccggctt tttgcggaac tggcgcagca gaccgagggc gccacgtcgg tcatcaagtt
     7861 cgtcaacatc cgcgagaccg gcggctggag ccgggacgcc tcccaggcca gccccaaaat
     7921 tgcggccctg cttgcggtcg cccacctgcc ggatgccgaa cccgtggcga ctgtgaccta
     7981 taaaagtgcg ggtcgcgtgc tcatcatcgg tgagctcgac gcggccgaac gcgccgccgg
     8041 cctgctgtcg gacgcgctgg acgtcacgct gttcagtctg gggacgggcc gcgacggcgg
     8101 tctgcaggag cggcgctacc cggtgctggc cgggaagatc cagcgcctga ccggctggct
     8161 gggagctttc gaactccatt ggcagcgcaa caacgcgatt gacctcgacc tgtgcacgcg
     8221 ctgcaacgcc tgtctggcgg cttgccccga aggcgccatt ggtttcgact accagatcga
     8281 caacgccctg tgcaagtcgc accgggcctg cgtcaaggct tgcggtgtgg ccggggcgat
     8341 tgatttcggc cgcgcacccg aggccttgag tgaaaaattc gatctggtgc tggatctgcg
     8401 tgcgaccccg gcctttaccc aacatgcctt gccccaaggt tatttccgct gggatggcca
     8461 ggacagccgc accctgttgc agctgcgttc gctcgtcggc gagttcgaaa agcccaagtt
     8521 cttcaactac aagcaaaagc tctgcgccca cagccgcaat gaaaaaaccg gctgcacggc
     8581 ctgtatcgat gtctgctcgg cctcggcgat aagcagtgag cgtgaccggc agcaaatcgt
     8641 tgtgaatccc aatctgtgtg tgggttgcgg tgcctgcacc acggtctgtc cgagcggtgc
     8701 cctgacgttt gcctacccgc gtcccgggga gcagggcgcg aagatcaaga ccctgctggg
     8761 cacttatgcg cgtaccggcg gcaagcaggc ggccctgctg ctgcacagcc aggaagcggg
     8821 catgcggtta ttgggcgatc tgggccgcct ggcgcggctc gatccggcca cgcatggcgt
     8881 gccggcacgc gttttgccgc tggccttgtg gcacaccgtg tcggtgggcc tggagttgtg
     8941 gctctcggcc atcgcccacg gcgcttccca ggtgtgggtc ctgatgacgg ccgaggaagc
     9001 gccgcagtac cgtgaggccg tgcgcgagca gatggacgtg gcgcaggcca tcctgggcgg
     9061 cctcggttac cagggggaac acttccgcat cctcgaagcc agggatgcac gcgacctggc
     9121 agcactcgat gccgagctgc aggcggcacc agcgcagggc gtggcccagg cggccggctt
     9181 tgccgtgcag gccgacaaac gtgccacgct ggagctggcg ctggatcacc tgatgacgca
     9241 gcaggccact gccaggcccg aggcgattgc attgccggcc gccggctcgc cgctgggcac
     9301 gctgtccatc aacaaggatg cctgcacctt gtgcctgagt tgcgtcagcg cctgcccggc
     9361 cagcgcgctg caggacaacc cggagcggcc ccagctcaaa ttcatcgaga aaaactgcgt
     9421 gcagtgcggc ctgtgcgcga cgacctgtcc ggaaaacgcc atcaccctgc agccgcgcct
     9481 gctgctcacg ccgcagcgca aggaggcgcg tgtgctcaac gagagccagc cctaccagtg
     9541 catacgctgc agcaaaccgt tcggcaccct caaggcgatc gagtccatgc tgggcaagct
     9601 ggccggccat gcaatgttcc agggggcggc tgccgaacgc ctgaaaatgt gcagcgactg
     9661 ccgcgtgatt gacatctact ctgccgacaa cgaaatcaag atcacggaca ttcgctaaat
     9721 ccatcccatg accctgcccg cccaactcgt accgtccacg ctggacgaag aaaccgcccg
     9781 cgccgaaatt tatggactgc tggccgcgct gtattacgca cctcccccgg ctgccctgct
     9841 cgacaacctt cgggtggccg tgaccgaagc gcctgcggcc ggcgccttgc tggaagcctc
     9901 gtgggctgaa ctggtcggcg ccgcccgcag cttgccggac ggggagattg cccgggaata
     9961 cgaggcgttg tttggcggcg tcggcaagcc cgaggtctat ctgttcggct cccattatct
    10021 cagcggcttt ctcaacgaga agccgctggc ggcgctgcgc accgatctgg aggcgctggg
    10081 cctggcgcgc gacgaagcca tgcccgaaac cgaagaccat atcgcctacc tgtgcgaggt
    10141 catgcgttac ctgattgcgg gggacgatgt ggcggtcgcc aacctgacgc gccagcgcga
    10201 gtttttcgca cgtcatctgc agccctgggc ggcgaccctg tgcgatgcgg tcagtgccca
    10261 tccgaaggcg gatttctacc gcagcctggc cggattcacg cagacctttc tcagcgttga
    10321 aacccagggc tttgacatgc tggcctgagg aggccgggtc aatgtcaggc acttcccgcg
    10381 cagcagattt tctcaaaatg cgacattttc ctttgactag aagcatcggg gtaaaaacca
    10441 agatacccta tcgttttccc ctaagtgttg cttttggctt ggcctgaccg ttgtcgcacg
    10501 cacaggtctg acgtaaagta ggcaggtcag taagtatgca aaccagttca taacagcaag
    10561 cttccttgga gaccactatg caggacagcc agccaaaacc ctcgcgccgg ggcttctttt
    10621 tcggcgctgc cgccaccggt gcagttgccg ccgtggcggt cgccctgccc ggagtgcaag
    10681 cgcccgaggc cgccctccag gaacccaagc ctgcgccgga aaaaggcggc ggctacagcc
    10741 tgtcggatca cgtcaagcgc tactaccaga ccgcgcgtat ctagttttcc ccgtttcccc
    10801 tcttacggag agctccatgc tgctcacgaa aaaaattcac ggtgttcacg gcgatacggc
    10861 gcagcgcgcc ggatctgcac gttttgtaca aagcctgtcg cgcggactgt cgaatgcgct
    10921 gcccaccatg gaccgccgcg gtttcctgcg ccgctcgggc ctgggggtcg gcgtcggtct
    10981 ggctgccacc cagctgacgc tggtcaagaa agccagggct gccacgcccg gcgcggggga
    11041 cggtagcgga aaaatcgagg tcaagcgcac ggtctgcacc cattgctcgg tgggttgtgc
    11101 cgtcgatgcg gtcgtcgaaa acggcgtctg ggtccgtcag gaaccggtgt tcgactcccc
    11161 gatcaacctc ggcgcgcact gcgccaaggg ggctgcgctg cgcgaacacg gccacggcga
    11221 ataccgcctg cgctacccga tgaaactcgt caacggcaag tacgaacgca tcggctggga
    11281 cacggcactc aatgaaatca gcgcacgcat gatggagttg cgcaaggcca gtggccctga
    11341 ttccgtgtac tgggtcggat cgtccaagca caacaacgag caggcctacc tgatgcgcaa
    11401 gttcgtgagc ttctggggaa gcaacaactg cgaccaccag gcgcgcatct gccattccac
    11461 cacggtggct ggcgttgcca acacatgggg ctacggcgcc atgaccaatt cgtacaacga
    11521 catgcaaaac agcaagtgcg cgttgtacat cggctccaac gccgccgaag cccacccggt
    11581 cagcctgctg cacatgctgc actccaagga aaccggctgc aagatgatcg tcgtggaccc
    11641 gcgtttcacg cgcaccgcag ccaaggccga cgagtacatc cgcatccgtt ccggctccga
    11701 tatcccgttc ctgttcggca tgctgtatca catcttcaag aatggctggg aggacaccaa
    11761 gtacatcaac gaccgtgtct acggcatgga caaggtcaag gaagacgtgc tcgcgaagtg
    11821 gacgccggac aaggtcgagg aagcctgcgg cgtgcccgag gcgcgcgtgt acatggccgc
    11881 cgagatgatg gccaaaaacc ggccgtccac catcgtctgg tgcatgggcc agacccagca
    11941 caccatcggc aacgccatgg tccgcgcctc ctgcatcctg cagctggccc ttggtaacgt
    12001 cggtgtctcc ggtggcggca ccaatatttt ccgcggccat gacaacgtgc agggtgcgac
    12061 cgatgtcggc cccaacccgg attcgctgcc gggttattac ggcctgctcg aaggttcgtg
    12121 gaaacatttc gccaagacct ggggcgtgga cttcgactgg atcaaggggc gttatgcctc
    12181 gcccgccatg atgggcaaac ccggcatcac ggtgtcgcgc tggatcgacg gcgtgatgga
    12241 gaaaaacgaa ttcatcgacc aggactccaa ccttcgcggt gtggtgttct ggggccatgc
    12301 gcccaactcc cagacccgtg gcctggagat gaaacgcgcc atggacaagc tggacctgct
    12361 ggtggtgatt gatccctatc cgtcggccac agccgccatg gcggccatgc caggcaaggc
    12421 cgaagacctc aaccccaacc gcgccgttta cctgctgccg gccgcgacgc agttcgagac
    12481 cagcggttcc gtgacggctt ccaaccgctc actgcagtgg cgtgaaaaag tcatcgagcc
    12541 gctgtgggaa agccgcagcg accacatgat catgcaccaa ctggccgaga aactgggctt
    12601 tgccaaggag ttgtcgaaga actacaagat gcagaaggtc aagggcatgg acgagcccgt
    12661 gccggaagac atcctgcgcg agatcaacaa atgcgtctgg accattggct acaccgggca
    12721 aagcccggag cgcctgaagg cccacatgcg caacatgcac atgttcgacg tcaagacgct
    12781 gaagtccaaa ggcggcaagg acaaggaaac gggctacgac acgacgggtg actatttcgg
    12841 tctgccatgg ccctgctacg gcacgccgga aatgaaacac cccggttccc ccaacctcta
    12901 cgacacctcc aaacacgtga tggacggcgg cggcaacttt cgcgccaatt tcggcgtcga
    12961 gcgcgaaggg aaaaacctgc tggccgaaga cggttcgcac tccaagggcg ccgacatcca
    13021 gaccggctac ccggaactcg accatctgct gctcaagaag ctcggctggt gggatgaact
    13081 gaccgagccg gagaaggccg ccgctgaggg caagaactgg aagactgact cctcaggcgg
    13141 actgatccgg gtgttcatgc agggccacgg ctgccatccc ttcggcaacg ccaaggcacg
    13201 ggcggtggtc tggaacttcc cggacgccat tccgcagcac cgcgaaccgc tgtacggcat
    13261 ccggccggat ctggtggcca agtacccgac gcatgatgac aagaaggcct tctggcgcct
    13321 gccgacactg tacaaaacca tccagcagaa aaacatcgct gacaaggtgc atgaaaaatt
    13381 cccgctgatc atgacctcgg gccgtctggt cgagtacgaa ggggggggcg aggaaacccg
    13441 ctccaacccc tggctggccg agctgcagca ggagatgttc atcgagatca atcccaaggt
    13501 ggctgccgag aagggtattc gcaatggcga acgcgcctgg gtcagcacgc cgacgggtgc
    13561 gcgcctgaat gtccaggcgc tggtgacgga gcgtgtggga cccgacaccg tattcctgcc
    13621 tttccacttt tcgggacgct ggcagggtgc cgacatgctg gcctactacc ccaagggcgc
    13681 cgcccctgtg gtgcgcggcg aagcggtcaa cacggcgacc acctacggtt acgacagcgt
    13741 gacgatgatg caggaaacca agacgacggt ctgcaacgtc gagaaggcat aaggaaatac
    13801 catggcacgc atgaaatttg tctgtgacgc ggagcgctgc atcgagtgca acggttgcgt
    13861 tactgcctgc aagaacgaaa acgaagtccc atggggcgtc aaccgccgtc gtgtggtgac
    13921 gctcaacgac ggtgtacccg gcgagaagtc catctccgtg gcctgcatgc attgcagcga
    13981 cgcgccctgc atggctgtgt gccccgtcaa ctgtttctac cgtaccgatg agggtgtggt
    14041 gctgcatgac aaggacgtct gcatcggctg tggctactgc tcctatgcct gtccgttcgg
    14101 cgcgccccag ttcccgtccc agggtacgtt cggcgtgcgc ggcaagatgg acaagtgcac
    14161 cttctgtgct ggtggccctg aggccaacgg cagccaggcc gaattcgaaa aatacggccg
    14221 caaccggctg gcggaaggca agctgccagc ctgtgccgag cagtgctcga ccaaggcgct
    14281 gctggccggc gatggcgatg tggttgccga catcttccgc acccgtgtgg tgcagcgcgg
    14341 caagggcgct gaagtgtggg gctggggaac ggcctacggt tcaaaacagg ctggccagcc
    14401 tcccgtgcaa ggagccaaat catgatgcgt ctcgcactgc ttacggctgc tgtcatggca
    14461 cttgccgctt gcggcgacaa accccagtcc gctggcggcg tcaagagcga cacggccgcg
    14521 tttcagggga ccggcaacgc cttcaatgcc ccgggctgga aagccggcga caagaccgcc
    14581 tgggagcagc agctcaagac ccgcgcgcag gccggtcaga acgaatacaa ccgcgtgaaa
    14641 taacagggag ttcacatgca acgcccatcg cgattcctgg cagcactcct ctttgcagcg
    14701 tttggcttct cggccacggt gtcggcgcaa cctgcggcca ccccggccgc agcagttgcg
    14761 gcacccgcac ccgcaggcgg tattcagagc gccaacatct tcgaggtcaa acccgaggcg
    14821 agcgctgatc ccaaatacgc cgagcagacc aatggcgagc gcgccagggt ccagcccggc
    14881 aacaatgcgc cgatgtggcg gcaggtcggc tccggcgtca ccggctacag tagcctgccc
    14941 aaaagcgagg cgcccgaggc cggcaacctg atccagcctt ttgtgcaata cccgggctcg
    15001 cgcttgacca atgccggtga ggcctggcgc caggtgcgca acaactggct gattccctac
    15061 ggtggcgcgc tgctgctgat cgtggtgggt gcgatcgcgt tgttttactt cggcaagggc
    15121 aggatcaatc tgcacggttc cgaaacgggc cgcaagatcg agcgtttcac gccgtttgag
    15181 cgttccgcac actgggccaa cgccattgct ttctgtattc ttgccatctc cggcgtcgtc
    15241 atggccttcg gcaagttctt cctgctaccc atcatgggca gcaccctgtt cggctggctg
    15301 acctatctgc tcaagaacat gcacaatttt gccggcccgc tgtttgccgt gtcgctggtg
    15361 attgtgtttt tcacctttgt ccgcgacaac ctgccacggc gtggtgacat gggctggctt
    15421 ctcaagggcg gtggtcttct ggccgccaag gaagtgccgt cacaccgctt caatgcgggc
    15481 gaaaaactgg tgttctgggg cggcgtgttt ttcctcggca tcatcgtggt ggcctcggga
    15541 ctggtgctcg acaaactgat ccccgggctg atctatgttc gcgacaccat gcaggtggcg
    15601 cacatggtgc atgccgtggc gacggtgctg atgatggcga tgttcatcgg ccatatctac
    15661 atcggcacca tcggcatgca gggtgcctac gacggcatga aaaccggcta tgtggacgag
    15721 acctgggcca gggagcacca cgaatactgg tatgacgacg tgaaggcagg caagattccg
    15781 gcacagcgca gcgagcctgc gccgcctggc cagaccgtcc aggcctagat cagattcgaa
    15841 cagcgaggat tccatgaaaa aaattcttat tgccgccttg tttgccggcg tgtccaccat
    15901 ggtgctggcc aagctccccc cggtcagcga tgaggccaag gccaaagccg ccgaggccgc
    15961 cgcgaaggcg gcctgggccg gcaaggtcga tggctacctg ttgtgcaaat ccatggacaa
    16021 ggtcgcggcc agttattaca agacggccaa ggcggccggc aaggaaacca agccgccggt
    16081 ggccacgcct gcgtgcgcag accctggcgc cttcagctac aagccgcctg aggcagccaa
    16141 gccccttgag gcggcaggtg cgcactctcc ggcagctaca gcaaccagcc cgcccagcag
    16201 caagacacct gcggcagcca ttgctcctga gaaaaaatcc tgaccatcgc ccatccattg
    16261 caaaaaggcc ggccccgcaa ggtgtcggcc tttttgttgt agtctccaac catgcctctg
    16321 ccatacctga cccaggcccg ggcgccgctc acctgcgaca tcgacgttgt caacgaatac
    16381 ggtgatccgc tccagattgc cattcccgcc gaacgccccc tcacggtgta tgtcgacaag
    16441 cgcgagctgg tgaccttgat gacactcggt gcccaccccg aactgctggt gctgggttac
    16501 ctgcgcaacc agcggctggt cagggacgcc gcagacattg aatccatcac ggtggactgg
    16561 gatgtggggg ccgcggcggt caagacgcgc cacggcatcg cggatatcga ggaaaaaacc
    16621 gcgcgcaagg tggtgaccac cggctgcggg cagggcagtg tgttcggcgg cctgatggac
    16681 gaggtggacc agatagcgct gccggccgat gcctgcatca cccaagccca gctctacggc
    16741 ctggtcaaca ccatccgcct caaggagacc acctacaagt cggccggatc cgtgcacgcc
    16801 tgcgcgctgt tttccctggc agccgcggag ccggagatgc tgctgtttgt cgaggacgtg
    16861 ggacggcaca acgccatcga caccattgcg ggctggatgg cgatcaattt gttggccccc
    16921 acgctcccca ctgcgcgtac cttcggcggt gcgctgcccc ttgcacggga aaaaggggcg
    16981 ctgcgcctgc ggcccggcga agccggttcc gcggccctgg ctggtgaaga gggcaccagt
    17041 ctggtgtttt acaccaccgg gcggctcacc agcgaaatgg tcatcaagtc ggcgcaaatg
    17101 ggcgtgccga tcgtggtctc gcgcagcggc atcacgcaga tgggccacca ggtggcgcag
    17161 gccgtcgggc tgtgtgccat aggtcgcgcg accaatcggc gttttgtctg ttacgcccag
    17221 ccgcagcggc tcaagctgca gccggacctc gccggcccca ggctccgggc gccagagacg
    17281 gcttgatggc ctgacggcgg tgcggcattc ccacagaggg ggcgacccgc tgaattgccc
    17341 gggctgttca ccggcagaaa ccgacaggcg ccggggctgt ggactttgcg ttatggtcat
    17401 ggcatgaaca cccttttcct caaactcggc tggcggacgt tgctgcgcga cctgcgcgcc
    17461 ggggaactgc gcctgctgat cgtcgcggtg acactggccg tcgccgccct gacggccgtc
    17521 ggtttttttg cggaccgcct caagggaggc ctgcagcgcg atgcccgcca gctgctcggc
    17581 ggtgacgccg ttcttcgcag cgacggcatg acccccgagg tgttcatcgc caaggcgcgc
    17641 agcctgggcc tgcaggtcac ctcgacagtg acctttccga ccatgggccg ggcgcgcgag
    17701 gaagacggcg gcgccagcaa acttgtggcc ttcaaggcgg tgcccgaggg ctaccccttg
    17761 cgcggcaccc tgcaggtcgc tgccagcccc gagactgccg gcgcggccac ccgcgagatt
    17821 cccgcccccg gcacggcctg ggccgacgca tcgctgctcg atgcactggg cctgcgcatc
    17881 ggccagaccc tgctgatggg cgacgcccgc ttcactttga cgcgtgtgat cgtccaggag
    17941 ccggaccgtg gcggcggttt tgccaatttt tcgccacgcg tgatgatcaa cagcgctgat
    18001 ctggcggcca ccggcctgat ccagcccgcc agccggacca actaccgctt tgccgtggcc
    18061 ggcagcgacg ccgcggtgaa agcctatgtc cagtgggcca cgcaggagat caagaaggcc
    18121 gagttgcgcg gcgtgcgcat cgagtcgctg gaaagcggca gccccgagat gcgccagacg
    18181 ctggaccgtg ccgaaaaatt cctcaacctg gtggccttgc tggcggcctt gctgagcgcc
    18241 gtggccgtgg ccatcgtggc acgcggcttt gcttccaaac acctggacga ctgcgccatg
    18301 ctgcgcgtgc tgggtcagcc gcaacgcacc atcgcgctgg cctacacctt tgaatttgtg
    18361 ctggccggca tggccgccag cgccctcggt gtgctgatcg gttttgccgt gcactatggt
    18421 tttgtgctgc tgctggccgg cctggttgag acagcgctgc cggcgcctac cgtctggccg
    18481 gtggctttcg gcatgggc
//
LOCUS       DAIDFG010000021        18494 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_21_length_18494_cov_17.045881, whole genome shotgun sequence.
ACCESSION   DAIDFG010000021 DAIDFG010000000
VERSION     DAIDFG010000021.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 18494)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18494)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18494
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21_length_18494_cov_17.045881"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3478 a         6028 c         5790 g         3198 t
ORIGIN      
        1 ccttcatgca cctgcaatat tcctatggtt acggcatggc cggacgcatg gccatgcagg
       61 gctatctggc cacggtcggc agcggcaaaa ccgggttcac cctggtgaac aacccggccg
      121 gtggggaaaa aacctatgtg gatggcatgc gcggtgcggt ggagcgcaac accatgcgct
      181 actacctggc gattgacgcg tacatggcgt cactggcggc gccgcctgcc gagcaacttg
      241 aaaaacgcct gcagcaatgg tttgacgcca cggaacgtta tccgcgccag ttgcatgaag
      301 tcgagcgcac ggcttacctc acgatgaagc gcaacgaaca ccggcgtcag caggcggccg
      361 aataaatccg gcaggttcga ctctggcagc tgcagggcca gacgtcagcg ggggcaccgt
      421 tgcggcgccc cacccttcct gtacttttta cttgcgcaac agctggcgcg cagcggccat
      481 gtcgcggtcg taaagcgcct tggcctcgcg ccggcaggct gtgcgttcgg cggcagccat
      541 cttgctgcat tcctcgaggt aaagcttgta gccaccaccg gcttcattga tggcggtgcg
      601 gtatttctgc tgcggcgtgc tgtcggcgac ggcgccgcgt tcgagcagcc gttcctccat
      661 ggccacaggc tgggccagaa cggcggagcc actgacaagc agcagggctg aaacaacaaa
      721 agcggagagt tttctggtca tggtgcgtcc ttgaatgact gaataaatga atagatggcg
      781 aggctgacag ggctggcgtt ttatttccgg cccgaactgt tgggcgtgtg ctggtgatcc
      841 agcggcggct tgtcgggcat gttggtcgat tcaatgcgtg ccttcacgct ggccgggctg
      901 ttggcggctt tttcggccag ggcctgctga ctgccactgg ctggctgggg ccccttgggg
      961 gtgttttgcg tcggcttgtg gccggacagg gcgggatgat ggttcatgac tgctctcctt
     1021 tgttgcccgc tggctggcag cggtattgag gttccttgcc cagcctactg ccgggtgacg
     1081 cgcaagcccg tcagcgcgaa ggtccgctgc ctgtaggaca gtcggaccct tctgccggtc
     1141 ttcagggcgt aaagagaaaa ggccgcccgc cctgcccgcc tgggtcaagg cgatgcgccg
     1201 cccgtagatc aagggcgggc ggtcatttgc ccgcgaattt caccacccgg aaacctggcg
     1261 gtgtggatgt tggcgtacca cttgccagcc atcaggtcgg caacctgggc cggtgtcagc
     1321 gtggcctggc cggacatcgg gctggtgatg gggccggtga aaggcagggc cgcgcctgca
     1381 ttggcgccca ctgcggccgg gccgtgaaag tggcctgcgg ttgcagggcc gctcaaaccc
     1441 gtgtagctca gttgccagcg caacagcttg gtgtccttgt tcaacaccgc atccacctgg
     1501 ccgctggcgt tggaggccac cggcggcacc tcgttggcgc cgcgcagttg cgtcgtgaat
     1561 gcaagcaggt tggactgcga cgacggcatc atgctgcaac cggaaaccag aaccaccgcg
     1621 gctgtggccg cgagggtgga acgaaatgcg cggcgacggg gaatcatcat gcttgtctcc
     1681 agtggaagga cgaaatttcc atcttaggac tgggcggcag agggcgctgt aggacgccgg
     1741 cttgatccaa accgcgggcc aggtgtcgcc tttgcacgat cattttctga aacgatgaac
     1801 aaaaaagcgc cccagcccaa caggtacgag cgcagatagc tataaattcc atagcacatc
     1861 aggcacatcc accgaccgct gcgggcctgc gccgtcgtcc tacggcagca acggacggct
     1921 gccgacagcg ccctgccgcc cctgcggatt aaggtcagac actgggtcac ccgcgtaatg
     1981 gagagttcag catggcaagc aggcatcagc accttccggg caagcccggc agcagcagga
     2041 ggcgatagcc atggcttcac gcgccttgtg gaaaggagcc atcagttttg gcctggtgca
     2101 cattccggtg tcactgcatt ctgcgaccgc cgacaacggg gtcgacttcg actggctgga
     2161 caaacgcagc atggacccgg tggggtacaa acgcatcaac aagaagaccg ggaaggagat
     2221 caccaaggaa cacatcgtcc ggggcgtgca gtacgaggac ggccaatacg tggtgctgag
     2281 cgacgaagag atctcggcgg cctttcccag gaccacccag acgatagaga tcgagagttt
     2341 tgtcgccaac accgacatcc cttttgtcta cctggaacgc ccctactaca ttgcgcccat
     2401 caacaagggc aaaaaggtct acgcgctgct gcgcgacacc ctgctcaaga ccggcagggt
     2461 cggcattgcc cgggtggtga tacagaccaa gcagcatctg gccgtgctgg tgccgtccgg
     2521 gcccgggctg attttgaacc tgctgcgctg gggcactgat atccgcggct gggacgagct
     2581 ggacttgccg ccagaaggcg ccaaggcggc cggcctgtcc gagaaggagt tgtccatggc
     2641 ccggcaactg gttgacgaca tgaccgacac ctggaagccg gagcagttca aggattcgtt
     2701 caccgatgcc atcacggccc tggtccagca gaaggtgaag caaggcaagg tcgaaaccgt
     2761 cgagcaaccg gaagcttctg atgcatcaac aggcactggg ggcgcaaaaa tcatcgacct
     2821 gaccgagctg ctgcagcgca gcctgcatca caagggcggc acagccggca agaccacggc
     2881 caaaacaccg gcaaaaacat cggccaaacc cgcaagcacc gcgccggccc gcaagccggc
     2941 cgccaaaacc ggcgccgcga gccggcgccg cgccgcctga acccgaagcc ggccacacac
     3001 atccatggct gccgatgacc cactccaggc ctaccgcgcc aagcgcgact tcaaacacac
     3061 accggagccg gtcacaggag gcacacgctc ccaggcccgc agcttcgtgg tccagaaaca
     3121 tgacgcgagc cagttgcact acgacttccg gcttgaactc gaaggcacgc tgaaaagctg
     3181 ggccgtcccc aaaggtccca gcctggaccc cggcgtcaaa cgcatggcgg tgcaggtgga
     3241 agaccatccg ctctcgtacg ccagcttcga gggcactatt cccgacaaac agtacggcgc
     3301 aggcaatgtc atcgtctggg atcgcggcac ctggctgcct gccggcgacc cggtcgcggg
     3361 gcttcagaac ggcaaactca agttcgagtt ggagggcgaa aaactccggg gtcactggtc
     3421 actggtgcgc atgcatggca aaagtgggga gcgccagccg ccctggctgc tgatcaagga
     3481 caaggacgac gcggcccggc cggccgccga gtacgacgtg ttgcaggcac tgcccgacag
     3541 cgtgatcacc ggcacgccgc tgacgccacg caaaagagcg gccgccaaaa aggctgcagc
     3601 caaagccgcc tccaccggca gcgctgccgc cagcaaaaaa acctccgcga aaccccgcct
     3661 gccgcagggc gccatcaagg acgacctgcc gcaaacgctg gcccctgagc tggcgacgct
     3721 ggtcacccaa ttgccgagtg acagccagga ctgggtgtac gagatcaagt tcgacggcta
     3781 ccgcctgctg acacgcgtgg aaggcccctc catccgctgc ttcacgcgca acgggcacga
     3841 ctggaccgcc aggctgccgg cactggtgga agccctgtct cggctgggcc tgcgctccgg
     3901 ctggctggac ggtgaaatcg tggtgcatgg caagaagggc gtgcctgact tcggcgcgct
     3961 gcaaaacgct tttgacagcg gcagcaccgc gcagatcgtc tattacgttt tcgacctgcc
     4021 gttttatgaa ggctacgacc tgcggccgct gccgctgctc gagcggcgca acatcctgcg
     4081 ggcgctggtg caggcggcag cgccgggcgc gatccgtttc agcgagacct ttgaggccgg
     4141 cacgcaggac ctgctcgacg ctgcacgcag tctggggctg gagggtgtga tcggcaaacg
     4201 ggcgtcatcg ccctatgtgt cgcgccgctc gccgagctgg atcaagctca agacacagct
     4261 gcgccaggag tttgtgattg gcggctacac cgatcccaag ggctcccgca ccggcctggg
     4321 cgccctgatg ctgggtgtgt atgacgacca gggcgccctg cgttatgcag gcaacgtggg
     4381 caccgggttt gacgacaaaa ccctgcaaaa gctgcagggc aagctggctg cgctggcgac
     4441 gccccgccct gcttttgccg gactgcccgc ctcggtcaag ggccactggg tccaaccggt
     4501 gctgctggcc gaggtcgttt ttgcacaatg gacgcacacc gggcgcatcc gccacgccgt
     4561 gttccagggt ctgcgcaccg acaaggacgc gcgcaaggtt acccgcgagt cggcgcagcc
     4621 cattgaggac ctcatgccca cgaacaaaaa acctgcggca gcgccaggca ccgcagccgc
     4681 caaaaaccgc agcacaaccc gcccggccgc ggacaccggc acgctgcgcg tcacccatcc
     4741 cgaacgcgtg gtggacccgg acaccggtat gaccaaactc gacctggtgc actactacgc
     4801 gctggtggca ccgctgatcc tgccgcattt gcgggccagg ccggtggcgc tggtgcgggc
     4861 gccggacggt gtccagggcg agctgttctt ccagaaacac gccgaggcga cggccatccc
     4921 cggcctcaag ctgctggacc cggcgctgga ccccggccat gcgcccttgc tggaaatcac
     4981 cacggcgaag gtgctgctgg ccgcctccca gctcaatgtc atggagttcc acacctggaa
     5041 tgccaccacc cggtccatga ccaaacccga tcgcatgacc tttgacctgg acccgggtga
     5101 aggtgtgtcg tgggcgcaga tccgcgaggc ggcccaactg gtccatgcgc tactgaagga
     5161 actcggcctg ccggcgtttc tcaagaccag cggcggcaag ggcctgcatg tggtggtgcc
     5221 gctgaaaccg gccttcagct gggacacggt caaggatttc tcgcaggcca tcgtgcggca
     5281 tctgtccgcg gtgatccccg agcgttttgt ggccaaaagc gggccgcgca accgcgtcgg
     5341 gaaaattttc cccgactacc tgcgcaacgg tttcggcgcc accacggtct gtgcctggtc
     5401 gctgcgggcc cggcccggac tcggtgtgtc ggtaccggtc ggctgggacg agctggacag
     5461 cctgagcggc ccggcgcact ggacggcggc caacatcgcc gatcgtgtgg ccaccggcaa
     5521 ctcgccttgg gagggctacg aacaagcccg ttgcaccctg cccgccgcca tgaaagcgct
     5581 cggcttcaag gcggacaaac cggtcgcatc accgaagccc tctcgggcgc gggcatctgc
     5641 ctaaaagctg caaaaaccga caagggccga cgaaggccga tgccgcggtg taggaccgcc
     5701 ttgacaagct cacgcgcgcc ttcctgacag gcgcttgggt tgcaaccggc ttcgcgcgcg
     5761 actgatcccg gcgtatcttc atgccaagtt ccggcggctt gcagcttgca ctctgcgcgg
     5821 ccggcggtct ggacactcga tgattttttc gggaacacgc catgaagcat tttttgacgg
     5881 cactgcctgt actttttgca ctgaccctgc tcaccgggtg cccgcagtcg aaggtgcccg
     5941 acaaaccctc gctggtgccc gagcccaagg cggccctgaa catcatgaac accccccaga
     6001 gttactcctt tgaaatctga cactccttct gcgccctgcc atccctggcg ccgatcttta
     6061 gccggtgtgg ctttggccgg cctggccgcc ctgttgtccg catgtgtggc gccgggctcc
     6121 cagctggagt caccgccgca gaccgtgcag ctcgacggtc atgcctaccg ggtcgagcag
     6181 atcacggcca gcacctggtc ggcgcaggcg ccgcgccacc tgcctgatgg cccccagaaa
     6241 aaggccaacc tggtccgggc cattgaaaag gcctcgagct gcaaggtgac cgacagcagt
     6301 tatggcgccg ggggcgtcgc actcaacgcc caggtggact gcggcagcag gctgaagaac
     6361 tagacaaatc acgctccagc cctcatccgg cctgcgcaaa cagctattga atcaatagca
     6421 actccaggcc tcggacaaag ctttacacct tcgcatccgc agcagacgca actttacgcg
     6481 tcgtgcggta tggcgctgca ggcaccccgc cacactcgca tcaacaaccc aaggagatct
     6541 tcatgatcgt caaccactcg ctcaaaaaaa tcatgcttgc catcgtgctg gccggtgcgg
     6601 ctgctgcacc ggccatcgca caggtcagca tcaacatcaa cctggcacca ccccagccgc
     6661 agtacgaggc cgtccccgtg cttgcacccg cgcaggtctg ggcaccgggc tactgggcct
     6721 ggaccggcga gcgccatgtc tgggtacgcg gtcgcgccat ccagaagcgc gacggctacc
     6781 gctgggcgcc ggaccgctgg gagcaacgcg gcgacggtta tcaccgccag ccgggcactt
     6841 gggtgcgcga tgccaactat gtgtacgtca aggataaaaa ggacaaaaag gacaagtacg
     6901 agaagtacga gaagtacgac aagcgcgaca aacacaatga caaacggcac ggcaacggga
     6961 acgggaacgg caaaggccac ggtaaccgtg actgatacct gaccccgctg tcatgacaca
     7021 aaaaaaagct ggcacttgtg ccagcttttt tgtgggtgaa acgaccgatc aggaagccgt
     7081 cttcagcgca tcacgcaggg ccttgacctg gtcgtggttg cgctgcacgc cctgggcctg
     7141 gcgctccacc agggtcctga tcgccggggg aagattttcc ttgagggcat tgcggtagcg
     7201 ggcctttgcc gcgtcctcgc cgcgctcgca ttcgtccagc atggccagat cggtgtaacc
     7261 gctgagcgtg ccgcgcaccg acacccagcc gcgatgcatg gcaccgctgg tcgtaccgcc
     7321 ttcttcgggc tcgccgccca gctggcggat ctgcccggac agctcggcag ccgcaccgcg
     7381 gcattcgtcg gcatgacgcc gcagcagggt tttgaggtcc tgcgccttca cgtgttcggc
     7441 gcattcggtg aaaccgtatt caccgtcgcg gcaggactcc agcaactcgt tgagcgtgtc
     7501 gatcacgtcg tcgttgtcgg cgatctcgcc agtcagcgtg cgcgatgaga gctggtcgtc
     7561 cacccgcgcc caggcggcgc ggctggccgc ctcggcttcg ggccagctca aggtcgagcg
     7621 ctctttgcgg ttgtcccaat gcacggccag gtcgctttgc gcatcgtcaa acgtaccggt
     7681 gtagttgctg cgcccgtaca ggcccaggcg gtaggccggt gcatagtcgt catagctgcg
     7741 gcttgcatcg tagtaggcct cccgggtgta ctgctcgcgc cagtaggctt cttcagcggt
     7801 ggggttgatg gcttcggcca ccgccttgcc gcccagtccg ccggccaccg cgccaacaac
     7861 cgctccggcc gcagcgccca ccggcccgcc caatgcgccg acggccgctc cggcggcagc
     7921 gccgcccagg gcggcaccca cgccggtacc caccgggtgg gcgcccggtg cgtcagtgat
     7981 cgggtccaga ttcaggttct tgtcgtgttc gtcaagtgac atggtgaagc tccttcaggg
     8041 gtaatgggat ggtccgtttg tggactggaa actgcgccgg tgatgccggg tgtcttgcga
     8101 tcgcagtttt tcagttcagg aggccatcgt ggtacgcgct tcaggggctc gcggtcaggc
     8161 gaggacaacc cggcctgtag gagaggacgc cgtgcagggc gccctgcaga agatgccgcg
     8221 ccgcatgcgc cggcaccagg ttcagtcgga caccaggctc agccagccag ctcggggtaa
     8281 cgccggaaaa tgccttcttc gttgaagggg atgcgtcgct cgctggcgag gtaggcggcc
     8341 acgcgccggt gttgcgccac ccgcgcgtgc aggccggcca ccagcggggc ttgctgcagg
     8401 gcgcgcccgg tggccagcgg aaaagcgtac aacaggccgg ccaccagctg gaacagcgac
     8461 agatcggcgt aactcaggcg tgcgcccacc aggcaggcgc tgctaccggg gttgcgcgcc
     8521 agcacgcttt cgaaccaggc gaaaaatttc ggaatgcgct cttcacgaaa agccttggcc
     8581 cgccgcacgg cctctttttt ctggtcttcg taatacaggc cggtggagat cgggtgatgg
     8641 gtgtcgtggg cttccatcac cgcgtcggca atcgtcagct gcagctggtg tgtccacaaa
     8701 cgctgcaatt catttttggc caccagaccc aggcgcgggc cgaggtagag caggatcgcc
     8761 gcggtctggc cgatgacggc cttgccgtcc accagaaagg gcggcgcaaa cggcgggtgc
     8821 acgacctgcg ggctgtcgag gtaatgcatc atggcggcga cgccctgccc ccggctttcg
     8881 ggaccgcgcg ccacgtccac gtagtcggcg ccggccgcct ccagcgccag ccggacaaac
     8941 tcgccgcggc cctggatggt gggccagtag tgcaactcat aaggcatgga ggtctccttg
     9001 tatcaccagg cattcatgaa aaaacaggct ttagcccttt acccatgggc gctggcagct
     9061 attcaattca tagcaaatca cgttcaatcc gccttcgcag ccagccgcca gagcgaggtg
     9121 acctccgccg ccctggcgac atgcagggca tcgctggcat cggcggcttt cggatgctgc
     9181 ggcttcacgt ctacacgtga cagcacctgc agccccgccg cggcgatcca gtccaacagc
     9241 tcgctgcgcg agcgcagacc caggtgttcg gccagatccg acaggatcag ccagccttcc
     9301 ccgcccggcg ccagatgcgc ggccagcccc tgcaggaaac cgcgcaacat gcggctctcc
     9361 gggtcgtaca ccgcatgctc gaccggcgag ctgggacgcg ccggcaccca gggcggattg
     9421 cagacgatca gcggcgcgcg gccgtcggga aacaggttgg cttcctgcag cgtgatgcgc
     9481 ccggccaggg ccagccgtgc caggttttcc cgggcgcatg tcagcgcgcg cgggtcctga
     9541 tcggtcgcga tcacctgctg cacaccgcgg cgcgccagga tcgccgccag cacgccggtg
     9601 ccggtcccga tgtcaaaggc caggctgttg gtgagggcgg ccggcggcaa gggtgtctcg
     9661 gccaccaggc tcaggtactc gccgcgcacc ggcgaaaaca cgccgtagtg cggatgaatg
     9721 cgcgctccgc ccagcgcggc gatctccacc cctttcttgc gccattcgtg cgcgccgatc
     9781 aggcccagca gttcgcgcaa tgaggccaca tagctgccct gcgccgcgtc gcccgggcca
     9841 tacgcttcgc tgcaggcctg cgcgacatcc ggcgcccgcc gcaggctgat gtgatgcgcc
     9901 ccatcgaact cgagcaccag catggccagc gtgcgcgcac gctgcgactg ggcctggcgg
     9961 tgctggttga aggcgtcgag cggagagacc gcggctgcct cagccctggc cttggctttc
    10021 ttgatctttt tgggcttgtc gatgcgcctg gccagggcct gcagcaattg ccgcgcattc
    10081 tggaaatccc cacgccacaa aatggccgtg ccctcgctgg ccaggcgaaa cgccgcgtcc
    10141 gcggtcaggg tgtcgtcggc cacctgcacc cgtctgggtg gcgccgcccc gctttcggaa
    10201 cgccagcggg ctgagcgggc ttgggcctcc tcgacccaat ggatgcgggc cggctcgctc
    10261 atgcgggggc tttcgatgaa agaacggagg tgggggcttt tgcccgggct ggcaaacgca
    10321 ggaagaaaaa aggcatcgga caaacccggt cggtgaacag accgccatta tcccgcgctg
    10381 tgcagcaagc acgcccttac agaggcttag cgactgtcct tgctgtcgcc gccggtcagc
    10441 ggcgcaaggt cggacgagta ggtgcggtca ccgatggcca tctcactgct gttttgccag
    10501 gcagactcaa aagccgccag ctcctgcgcc gtcgcgcgtt cgtagcgacg caggttcgag
    10561 gcccgcatcg cctcggggct gatggcgtcc ggcgcggcgg tggcttcggc gcgctgggga
    10621 tcgaagccct tgagctcctc gttgacccgc cagtacacag ccggcacgcc caggccataa
    10681 cgggccagtg cgttttttcc gatgacggcg cccaacgcag ccaggttttt ttgctgcccc
    10741 tgctcgcttt ccatgaaatc ggtggacaag tccaccatca cgacgaagct gtgaccgcgc
    10801 ttgtcggcgc gtacgacctt gaagcggtag cttgcactga ggatgccccg gctttccatg
    10861 gtggccttga ccgaatcaaa aagcatctcg cggcgaaacg ccatgcgttc gtcgagattc
    10921 attttgacgg ggccagtctc cggtcccgaa ccgccttcct tgtggccctg cgaggaagag
    10981 aataagtttt ggaagaacga catggcgtgc tgaatgttga atgacgtgtg ccaaccctgt
    11041 tgtcaatcta gttatgttgc ctatggtgtt gtcatgaggg cttttctggg gggaagccgc
    11101 ccttgacaac ataagcatcg tatgctttta gttacagctt gtcatcttct gatacacggt
    11161 aatctgacgg tacgcggctg tgacaaatgt caggaaacag gtcctaaggc gtcaaaacct
    11221 gcatttcagt aaaacttctg gtttagcacc gcgcgtcgcc agccgtcggt tttctccgac
    11281 atggcaaaaa gcctgcgccc gataggtggg gcgagcgtcc ttgcagatac ttgcccatca
    11341 acttctggag gaagcaatgt cttttgcgct ttatatggtc ggtttcgcaa tttttatggg
    11401 tggtctggtc tggggggcgt tcgaagccgg cgtcccccct gtctacattg gcatcggagc
    11461 gctcgtgatt ctgggcatag gcatcttcag cgctgtgggc cgcacccgca gcaaggatcc
    11521 ctcttgatga aaaacctgtt ggcgaattga gctggcaatg ctggcgggcc ttccgccacg
    11581 gcgccatcac aggggcgccc atcgtgcctg tcacgccccg cttcgagcgg ggctttttta
    11641 tgcccctgcg ggcaagaggc cgacacagtc atgacgcaac agtaggcgct gtcccacagc
    11701 ttcatgcggg ctgcaccgat acggcacccc cacccacggc ccaatgcttg accatcgcgg
    11761 cgccaatggg ccgcgatgcc atccctcccc tatcaacgca tcggctcttg tttgaaggac
    11821 acgccatgaa actcacccac atcagaacct tttctgccgc cgcagccgcg cttgcctgca
    11881 tgagcccggc attgctgatg gcccagatgg ttgacaaccc gtcaacctac atcatgccca
    11941 gcatcagcgt ggtggatcct gatgaccgat tcaacggcgg caagaatttc aatgcgctgg
    12001 gcctgcgcct cggtaaagcg gtgtcgccgt catgggacat ccaggggggc ggcacgctgg
    12061 gccggtcgac cgagaacggc atccgttacg agcaggccac actgggcgtc gatgccctgt
    12121 acatgttcaa ccgcagcaac ttccgcccct tcctgctcgc cggaattggc gcgcagcgcg
    12181 acaaactgcc cacagccagt gccacctcgc cttacctgag cttgggcgcc ggcttccagg
    12241 tggccctgtc cgagcagtgg ttcatgcagg ccgacctgcg ccgcagccgc gcttacgttc
    12301 gccacaacac cttcggcgcc gaccacctca cgaccaattc gctgaacttc ggcctgagct
    12361 actacttcga caagccagtg cgcacccagg tggcgcagta cacaccaccg ccggcaccgg
    12421 cgcctgtcat ggcacccccg cctccaccgc cggccgccgc gccggcacgc tttgagcgtg
    12481 tgaccctgtc ggccaccgaa ctgtttgcct tcgacagcgc ccagttgcgc atgccgcaga
    12541 ccaagctcga cgagattgca caggtcctgg gggccggcca atacagtggc aatgtcaccg
    12601 tgaccggcta caccgaccgt ctgggttcgg acgcctacaa ccagaagctg tcgcagcagc
    12661 gcgccgaagc cgtgcggaac tacctgacca gcaagggcgt ggcggccaat cgcctgacag
    12721 ccgtcggcaa gggcgaagcc aacccggtcg tcacctgcaa tgaccgcaaa cgcacggacc
    12781 tgatcaattg cctggcaccc aaccgccgtg ttgaagtcga ggaaatcgtc atcgagcgcc
    12841 gcgtcaatta agcgggcctg accacaggaa aagggttccg ggattctccc ggagcccttt
    12901 tttttgtccc gttgccgtca tcaggacggc ttggcgccca ggcccatggc tcgggtgatc
    12961 acttccttca tgatttcgtt ggtgccgccg tagatgcgct gcacgcgtgc gtcggcatag
    13021 gcacgggtga tcgggtactc ccacatgtag ccgtagccgc cgaaaagctg cacacactcg
    13081 tccatcacct tgcactgcag gtccgagcac cagtacttgg ccatgctggc cgtcgcggta
    13141 tcgagcttgt catcggcgag caactcggtg catttgtcca caaacacgcg tgccacctgc
    13201 acttcggtct gcagctcggc cagggtgtaa cgcgtgttct ggtaactggc caccggctgg
    13261 ccgaagacct tgcgttcctt cacatagtcg accgtccagc cgatcgccgc ctgcgccgca
    13321 gcgaccgcac cgatcgcgat ctgcagccgc tcccagggca gttgttccat caggcagatg
    13381 aagccacggt tttcctgggc ggtaccaccc agcaggtttt ccgcgggaac acgcacattg
    13441 tcaaaaaaca gctccgaggt gtcctgcgcc ttcaggccca gcttcttcag gcgtttgcct
    13501 accgcaaaac ccggcatgcc gcgctcgacc agcagtaagc tggtgccctt ggcgccggcc
    13561 gcggggttgg tcttggccac cacaaccacc aggtcggcat gccagccgtt ggtgatgaag
    13621 gtcttgctgc cattgagcag gaagctgccg tcggcctgct ggagggccgt ggacttgacg
    13681 ccctgcaggt cggagccggc cgacggctcg ctcatggcga tggcgcccac catctcgccg
    13741 ctggcaagtt tgggcaggta ttttttcttc tgcgcctcgg tgccgtaatg caggatgtag
    13801 ggcgccacaa tctcgctgtg caggccgtaa ccgatgccgg aaaagcccgc ggtgcttagc
    13861 tcttccatct gcaccacgga atacagcttg tcggcgccag cgccgccgta ttcctcgggc
    13921 atggtcatgc acaggaagcc gttctccccg gccttgttcc agaccgcgcg gtccacataa
    13981 ccctgttcct cccataacgc gtggaaaggt gcaatttcct tgtccatgaa gcggcgaaag
    14041 ctgtctcgaa aggcctcgtg gtcgctgctg aaaagtgtgc gttcgatcat ggggtcacct
    14101 gttggaagaa gtcaaagagt aaaaagcaga tggagggctg agcgtggggg ccatatttat
    14161 gcaaagcatt tgcttggtga aatgaatata ctcgttcctg cggcttttcg gcgcagaaac
    14221 cggcacctcc ccttgccggc actgccctcc ccccattcag caggagcacc ccatgaccga
    14281 agcattcatt tacgacgcca tccgcacccc ccgtggcaaa ggcaaaaagg acggcagcct
    14341 gtacgaggtc aagccggtca acctgctggc cggtgtgctg accgagctgc agcgccgcaa
    14401 caactttgac accgcgcaag tcgatgacgt ggtgatgggt gtggtctcgc cgattggcga
    14461 acagggctcc gtcatcgcca aggtggctgc gctcaaagcc ggctgggact tccgcgcctc
    14521 tggcgtgcag atcaaccgtt tttgcgcctc cggcctggaa gccgtcaacc tggccgcgca
    14581 gaaggtacgt tcgggttggg aagagctggt ggtggccggt ggcgtcgaaa gcatgagccg
    14641 cgtgcccatc ggttcggacg gcggcgcctg ggcccaggac ccggagacca acagtgccac
    14701 cggttttgtg ccgcaagggg ttggggccga cctgatcgcc acgattgaag gtttcagccg
    14761 cgccgatgtc gacgcttttg cgctggagag tcagaagcgc gcggcccagg cccgggcggc
    14821 cggccatttc gaccgctcgg tcgtgccggt aaaggacttc ctcgaccaga ccatcctggc
    14881 gcaggacgag ttcatcaagc cgcacaccac gctggaaggc ctggcctcgc tgcggccggc
    14941 ttttgaacag atgggcgcca tgggtttcga ccaggtcgcc ctgacccgct acccgcaggt
    15001 cgagcgcatc caccatgtgc accacgccgg caactcgtcg ggcatcgtgg acggcgcagc
    15061 ggcggtgctg gtcggctccg aagccgccgg aaaaatccat ggcctcacgc cgcgcgcgcg
    15121 tattgttgcg gcggccctca gcggcgccga cccgaccatc atgctgaccg gccccatgcc
    15181 ggcggctcgc aaggcgctgg ccaaggccgg cttgggcgta gaccagatcg acctgtttga
    15241 agtcaacgaa gcctttgccg cggtggtgat gcgtttcatg aaagagatga aggtgccgca
    15301 cgacaaggtc aacgtcaacg gcggcgcgat cgccatgggc cacccgctgg gcgcaaccgg
    15361 cgcgatgatt ttgggcaccc tgatcgacga gctgcacagg cgcaagctgc gttacggcct
    15421 ggccacgctg tgtgtgggcg gcggcatggg cattgcaacg attgtggaag ccatttaaac
    15481 cctgtcatgc cgggcttgac ccggcatcca cagcccgccg acatatgaca ttcacccaac
    15541 atggattgcg ggtcgagccc gcaatgacaa acagatgaaa acaatcaagt acgaactgac
    15601 cgacggcatt gccaccctca ccttcgacga ggaaggctca ccggtcaaca ccatgtgtgc
    15661 ccagtggcag aaagacctga ccgaagccac tgcacaggtg gtcaaggaca aggacgcgat
    15721 tcgcggcatc gtgctggcgt ccaacaaaag caccttcttt gccggcgccg atctcaaggc
    15781 cacgatgcgc ctgaaggcat cggatgccgc ggcggtgttc caggaaatcg agcagaccaa
    15841 aaagaacttc cgcacgctgg agaccctggg caagccggtg gtcagttgcc tcaatggcac
    15901 ggcgctgggc ggcggctggg aagtggcact ggttggccat taccgtattg ccgtcgatga
    15961 caagaaaatc cagttcggcc tgcctgaaat cacgctgggc ctgatccccg gcgccagcgg
    16021 catcaccaag atgacgcggc tgctgggcct gatgggcgcg cagccctata ttctggaaag
    16081 caaactgttc aacccgcatg aagcgctgga gcttggcctg gtgcaagaac tggtgccgaa
    16141 tgccgccgcg ctgcgccctg cggcgctggc ctggatcgcg acgcatgatg gcgccgccgc
    16201 gccggcgcac cacccctggg acgacaagaa ctacaggatg cctggcggca cgccggccaa
    16261 ccccaagatc gccggcatgc tggccgtggc gccggccgtg ctcaagcaga aaacacgcgg
    16321 cctgtacccg gcccccgaat atgcgctggc cgccatggtc gagggtgcgc aggtcgattt
    16381 cgacacggcg ctgcgcatcg aatcgcgtta cctggcccgg ctgatcgtga gcccggtggc
    16441 caaaaacatg atcaacacct tcttcttcaa catgaacgcg atcaagtcgg gccgctcgcg
    16501 tccgaaagat gtcccgcgtt acaagccgca gaaagtcggc ctgctcggcg ccggcatgat
    16561 gggtgcgggc attgcctacg cccaggccag ccgcggcatc agcaccgtgc tcagggatgt
    16621 gagccaggac aaggccgatg cgggcaaggc ctacagcgcc aaactgacgc aggcacgcgt
    16681 ggacaagggc cgcatgagtg tgcccgatca ggcggccctg ctcgcgcgca tcagcgccac
    16741 cgacaaggtg gccgacctcg ccggctgcga tctgatcatc gaagccgtgt tcgagcgccg
    16801 cgacctgaaa gccaaggtca cgcaggaagc ggagcccttg ctggcacctg gcggcttttt
    16861 tgcgtccaac acctccaccc tgccgatcag cgggctggcc acggcgagct cgcacccgga
    16921 caaattcatc ggcatccact ttttcagccc ggtggacaag atgaagctgg tcgagatcat
    16981 ccgcggcaag caaaccgacg acgacaccgt ggcgcgtgcc tttgactatg tgcaggccct
    17041 gggcaaaatt ccgattgtgg tcaacgactc gcgcggtttt tataccagcc gcaccttcgg
    17101 cacctttgtg atggaaggcg cggccatgct gggcgaaggc attcccgcgg cagcaattga
    17161 aaacgccggc atccagtgcg gcatgccggt gggcccgctc gccgtgctcg acgaaaccgc
    17221 gctctcactg agcgtgcatg tgctggacca gacccgcgcc gactacgtgg ccgaaggcaa
    17281 gacctacatc gccacgccag gcgagctgat cgtcgagcgc atggtcaagg agttcgaccg
    17341 caatggccgg gccgcaggcg ccggcttcta cgactacccc gaggaaaaag gcgcgaagaa
    17401 aaccctgtgg cctgaactca agcccctgtt cgaaaaaccc gatgcggcct gggccatcac
    17461 cgacctgaaa gacaggctgc tgtaccgcca ggccgtggag accgcgcgct gcctcagcga
    17521 aaacgtgctg accagcgtgc acgacgccaa catcggctcc atcttcggca ttggttttcc
    17581 cgcctggacc ggcggcgcga tgcagttcat ctacagcatg ggcgtggacg cctttgtgca
    17641 gcgcgccggc gaactggctg cccagtacgg tccgggtttt gcgctcagcg acgaggtcaa
    17701 ggccacgatc agaaagcacc agccggttta ttgagggttg attgcgctgc gaggccggcc
    17761 agcaggccgg ctttctgcac cttgcccagc gcggttttgg gcaaggcctc gcagaacacg
    17821 atgcgcttgg ggtgtttgaa gcgtgcgagc cggccctcga acagttgcaa cacctgcgcc
    17881 gcgtcgagat ccgcgccggg ggctttcaca atgacggcaa ccaccacctc tccccagcgc
    17941 gcatcgggct ggccgaccac cgcggcttcc aggatctgcg ggcagtcggc cagcaggttt
    18001 tcaatctcgg ccgggtagac gttttcgcca ccggagatga tcatgtcttt cgagcgtccc
    18061 accacctcgt ggaacccggc gtcatcgcag cgcgccaggt cgccggtgtg aaaccagcca
    18121 tcggcaaaag ccgggttgtt gtcatcggcc caatagccac acatcacatt gggcgcacgt
    18181 acgcagattt caccgacctc gcccggggcc acggtttcgc cctgcgtgtt gactagcttc
    18241 acgtccacat gcagcccggc cttgcccgcc gcaccgacgt gctgcatcgc atcggcacgg
    18301 cgcaggtaga tggagaccgg gccggtttcg gtcgcgccat agacctggcc aagcggcacg
    18361 ccgcgcgcat ggatggccgc aatcattgag gccggaatcg tggacgagcc cgctgccaga
    18421 aaacgcaagg atgaaagatc ggctgaagcg aaatccgcat ggtccagcac cgcgcgcaag
    18481 gtggccggca ccat
//
LOCUS       DAIDFG010000022        18373 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_22_length_18373_cov_18.154384, whole genome shotgun sequence.
ACCESSION   DAIDFG010000022 DAIDFG010000000
VERSION     DAIDFG010000022.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 18373)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18373)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18373
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22_length_18373_cov_18.154384"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3608 a         5654 c         5300 g         3811 t
ORIGIN      
        1 gcgcttgcgg ctgatgtgaa cgatcacggc ttctgtcgca aaatcgacga actccttggt
       61 gtagccaagt tcagccaatg cccagcgaaa atcatttcca agtcgggctc tggacagcca
      121 gccgagcttg tggcggtcgt ggaattcaac cgctttaata caggcatctt ccgttatgcg
      181 ggtaaggcga tttacggaag ggcgctttcc tggctggcta tccagggcag gagggtatcg
      241 cttggcgatc tggtctgcca gatccaccgc aaaatctttt atttcggatt ttcgccctat
      301 gcgaagaaag ctcaacatcg aaagtcctga cttgtggaag ggcacatcca tgtgcccttc
      361 cacaatctaa agttaagcaa tcaggtaccg cggcaagaag caggtgcgta cttggcaatg
      421 gatgcgttgc aagtccagac cacggtttgg ccggtccagc tcggtgtcaa ggtgacgata
      481 aacgaatccg taccaatcgc actcttggaa gtcacagcaa tcgttcccga agtatccacc
      541 gaaccggtag acacaaattt cgaggaggaa ataccaagcg caatggcgct ggcagcagtc
      601 gcgccaattg ccatggtgtt ggccagttgt gccgcttcag tcacacccgt gcgggcggaa
      661 gaaccagcca ggatcacctc agaagcgcga gcgcggatgg tgtagtcctg gtacgcaggc
      721 agtgccactg cggccagaat gccgataatc gccacgacga tcatcagctc gataagggta
      781 aaacccttct gcatagattt cataaaaact ccttgaaaat taaacccaac ctagtgcgcg
      841 acgaaagtct ggtcgtgccc attgagggca gcaactcttg tgccatgcgg aaaggggctg
      901 gattcattga tttttagggt ggaaagctac tttttagtca cgctgcccga caaatttgtc
      961 aggcaccaaa atccctttca ggcaccgggt agcctcgctg ataaccccgg gccagtcatt
     1021 gtgatgcggt tgccgaagca ccctggcatc agggtaccaa ggcatctggg tgctgttcag
     1081 gccataacgc cattccggaa tgtgcggcgc aagcaccagc acctttttgc ccagcgcgcc
     1141 agcatagtga acggtggtat tgcaaacagt caccacacaa tcgagggctt caattaaggc
     1201 agcaaactcg tccaagtcag caattgcctc gttccagtgt gtcagcatga cattcgacgc
     1261 tgctgcaatt ttcaggtctt cgtccaccga gccatactgg aggctgatcc aggtcgcact
     1321 cactgcattc gtcagcggcg ccagctcact gggtgacatc gagcgcagga tgcgtcgggt
     1381 agcttgtaaa ccgccgcgcc aggagacgcc aatccacggc ctgggacctg tcgcttcaag
     1441 tttctggcgc cagtaagcca cacgatccgg atcagccttc aggtaagcct tacccggaaa
     1501 agccccgacg cttgtcctga agtactgggg caaatctccg aggggcacga agtaatcgac
     1561 atcccccacc gttttggccc actccatctt cttgtcctgc ttggtgtgga cgatttcaca
     1621 ttggggaaac gagcgggcca gcgtgggcgc aacccgtcca atcacctcga ccacaacgcg
     1681 tgccggcccc aaggccaaca gatcaggtaa acaagacgcc aacatcacct gatcgcccaa
     1741 gccctgctcg gccagcacca caatacattt cccggaaata ttctggccgc gccatttctt
     1801 gaatggcaac acccgatact gacgggtata cgccaatcca cggtacgcat aacctgccca
     1861 cccctctgca aaattaccca acaacagatt gatctgggcg cgctgcaaat gcaaattggc
     1921 gtcccggggc tggattgcca gggcttcatt cagcaaagcc atcgccttgt cgaaatgtcc
     1981 gctgtaagcc tgagctaccg cgtaggagac cagaaacacg ctctttcggt ccggctcctt
     2041 ctcgatcaga tcgtcaaaca atgcaatggc ttcctcgtag tgacaagccg tcgacaatgt
     2101 tgcagccagc tgggcctgat ggaggaagtt aggctccgtc tcaaacaccc gccgcctgac
     2161 ctcaagggcc tcagcgtgtt tttccaggtt gctcagacat tgcgccaagt cgcaaagcac
     2221 cttgatgttt tcgaatcctt cctccaaggc acgattcaaa tatcgctcag catcggcatt
     2281 ttcgttggcg gcaaggagcg cggctccaag tgcggcaagg gcttgcccat cctgggggtg
     2341 gcccaccaga taagcccgca aaacgtccat gccgctgcgg aaatcgccca gcttacgata
     2401 cagagcatcg agttcacaca ccgggtctgc aagatctgcg tattccatgc aaatggcttc
     2461 ggctcgggcg agagaggcat ccgtttcacc ggcctttctc aaaacatcca cttcatccca
     2521 aagccgccgc caaggcgcgc gctcctcatc gacggtgctc ctggaggtcg gctcgggcgg
     2581 catgatcgct ggcgtaaaca ctgccccaac cgcactcatc tccccctcca gataggccat
     2641 gttttgcaag gcgagggcga acttgggatc cagcttcaga gccttggaaa aacatgcgtg
     2701 agcatccccc ggctcctgtt tggccaggta gaccagtccc aggttgttcc aagcagcttt
     2761 gtccttgtcg ttcaaacgca aggcttgggt cagcggcaat tcagcgcttg gaaaattccc
     2821 catcgtgacc tgacagcacc cgagttgagc gaggtagcgc ggatcgttgg tagtgacgtt
     2881 tttcgcgaat tgagcgtgtt caatggcggc ttccgtatct ccctgctcca gtaaaagaag
     2941 ggaaagaccg aaatgtgcgt ccgcaaagcc gggggcttct ttaagaaccg cccgaaaacc
     3001 gtcttccgca tcagcatccc gcatctggcg aaaagcaatt tcggcaagaa tgcagtccct
     3061 ggtcaagtcg cgtcgaggcg ttgccgcatg aagctgatcc gcagcttcct gcgcggccgg
     3121 gaagtcacca ctatcaatca gctcacgaac ctcttccact gtccaaaagc gtttccccga
     3181 tgaaaagagt gtgcgtacta actgtttgag catgagccag atccagacga aagggaaaac
     3241 tgcatcatat cgatgcgata aggctattta cctgaaaacc agccttataa cactaggaca
     3301 tttgcttatt tgatgcagac cgaccgaacc tgcttgaggt cggtcagccc catcagcact
     3361 ttctccatcc cgtccatttt cagtgtgtgc atgccgctgt tgacggccgc cgcaaacagc
     3421 tctgccacgc gggcacgctc ctgaatcagc tttttcgccg gatcgtcggc caccatcagc
     3481 tcatgcaggc cgatgcggcc cttgtagccg gtctggttac atttatcaca gccaacggcc
     3541 cggtacatgg taagtaggcc atctttgcca tagcgctcca tccagtcgtc cagcagcttc
     3601 tgtgcttcgc cgccgggatc ggtcttccag gcttccgtgt tccgcaattc ttccgaatac
     3661 tccctgatga agtccttgac ttcctgtgag ctcggctggt aagcctgctt gcagtcacaa
     3721 agctttttgg ccaggcgctg ggccaggatg ccgagcaggg catcggcaaa attgaaaggg
     3781 tccatgccca tgtccagcag ccgggtgatg gactcagggg cagaattggt gtgcagcgtc
     3841 gagaacacca ggtggccggt caacgaggcc tccacgccca tggacacggt ttccttgtcg
     3901 cgcgactcgc cgaccatgat gatgtccggg tcagcacgca gaaaagcacg catcaccagg
     3961 gcaaagtcga tgcctgcctt cctgttgatc tggacctggc gcaagccctt ctgcgttatt
     4021 tcgaccgggt cttcagccgt ccatatcttg gtgtctggcg tgttcagaaa tttcaggatc
     4081 gagtgcagcg tcgttgtttt gcctgaaccc gtcgggccac aaacgtaaaa aaggccatag
     4141 ggcttggaaa cggttttctc cagccgctcc ctgttgtgat ccgtcagtcc cagcttttca
     4201 agcgggatgg gttcgcccgc agcgagtatc cgcatgacga catcctcgac acctcccgct
     4261 gacggaatgg tggcaacacg cagttcgatg tcgagcggtc cgtatttctt gaacttgatt
     4321 ttcccgtcct gcggcttgcg cttttcggaa atgtccagat cgcacatgat cttgagccgc
     4381 gtgaccatgg cctggcgaaa ctgggccggc acctcgatgt agggcagcaa gcttccgtca
     4441 atgcgaaaac ggatgccggt cttggccttg cccggcatgg gctcgatgtg aatgtcggag
     4501 gccttctgct tgtaggcatc aatgatgacc ttgttgacaa acttgaccag ctcgttgtcg
     4561 gcggcggccg actccagcgc cgaatcgtcg ttggcatcgt tttcgagcgg cgagtccatg
     4621 tcggccagaa gctggtcaat ggagccgccc tcgttgcccg cgccaaaaag ctggctcagt
     4681 gtttcgtcaa attcggtctg ggtggtcacg cagtaggcga acttgctgat tttcgggaat
     4741 acctgcggga ccacccgggc tccccgcacc gcctcgggat caacacacat gatgaccaga
     4801 ccgtccggcg tttcctccag cggcatccag ccctgctgct ccacaaagtg ccgcttgagc
     4861 aggccatgca gcatttccga acgtattcgt cccgcgttgt aggcctcgta gggaacacca
     4921 aagaacttgc tcagcgacgc gccaatctgc gcgggacgaa tacggtaatc agccatcagc
     4981 agatgttcga ccgactggac ctcgtcgcgc gccttctgga tgcagtggag cagttcgtcc
     5041 tggctcaaca ccccttcggt gaccagcccg tcgtacttgg tcgccctgcg ccgctgggcg
     5101 tcttcggcct tctgcatgcg ctggcgaatg gcgatgccca gcgtctggca cagttgctgc
     5161 gcaccgtctt cttccagctt cgcaaaaggc tggtcgctgc ggttgttgat cacctgcagg
     5221 acaccataaa gcgtgctgcc gtccatcacc ggcgctacaa gcatctgttt ggtgcggtaa
     5281 ccggagcgtt tgtccacctc ctgcagaaaa ctcagattgg gatggatttt cttgagcgct
     5341 tcgtcgtcat acacatcggc gatattcacc atggttttcg acagcgcaac gtagcccgca
     5401 atgctttgcg cgctgatggg cagcttcagg tcgcggctgg tgttgagccc cgtcttgacc
     5461 ttggagatga tggacgtgcg gtcttcattg acggcgtaga gggtcaggcg gtcggcattg
     5521 aacagcttgc agatatcctg gctggcctcc agcatgattt ggtcaatgtt ctcggtttca
     5581 tgaatgcggt tggtgaccag ttgcagctgg cgaaaataaa gcgcttcaaa agtcactccc
     5641 cgctcctgcg gtggaagcat ctgcgagtca aaaaacgcgt cttcgtggtc ttgctgtaca
     5701 acggcattca taactcaaac tcctggcccg cgcgcaacca gcgtatgtcg tgggtaacgt
     5761 tgggaccaaa cggcgccgtc tggtcaaagc gctggatctc ggccatgatc aagtcagtct
     5821 ccgccggctt ggtatgggtg atgtaaatcg gatagttcct gcctttgtcg atgcagtcca
     5881 gctcgctggc cagcgcatga ggcgacagat gcaggctacg ccgggccaga tcgcgttcgc
     5941 ggttgctgaa ggccgtctcg atcaccagca tggcgacatt gagctggttg atccgggccc
     6001 acagcgcggg gttgcgctcg gtgtcgccgg tgaacaccca gcacccggcg ccggtggcca
     6061 ccgcataacc caccgcgggc acagtatgaa ccgcaggcag cacttccaca tattttcccg
     6121 ccagcagcag cgtctggccg acggtcagcg gatgaaagct gacaaacggc gcttccggcg
     6181 tggggattct tgagaaatca ggccagatgg tgttgttgaa aatatgggtt ttcagcgcgt
     6241 cgatcgtgcc ctgcagcgca tgaatctgta tcggggtcgt ccgttgtgcc gcaatcgcgt
     6301 ccaccatcag cggcaaggcc gccacatggt ccaggtggga gtgtgtcagc agcacgtgat
     6361 caacgccgcg catctcgtcc agcgtgagat cgccaacgcc cgtcccggca tcgaccagca
     6421 cgtcgtgatc cagcaggaaa gacgtggtcc ggcagccttt ggcaatcgct cccgaacagc
     6481 ccagtacgcg tactttcatg gtcttttttg gcttgaggcg ccggcaggct gtgtcatttg
     6541 gtttcgaagt tggttgcccc gtcccactcg ggatcaaacg gcaacaacct catggaggct
     6601 acacggtctg catacagccg gtaaaggtac ttttgcgcat tcatgcgctg taagttgagc
     6661 aaatgcacat cgcagtcgtc ccaggcctgg tcgcggtagg ccctgagcgc caacgcccac
     6721 tctctcagtt cgctgcccag ctgcttgtcg agctggccgg gaagcgccac cggacagaaa
     6781 atggccaccg cctggtcctt gcccttgacc cggaccttgt cgagctcctg ccaggcaaac
     6841 ccggtggcca gttcgcgggt ggacgcactg accacggtat cgacgccata gattttggac
     6901 agtccttcca gacgagaccc caggttgacg gcgtcgccaa tgacggtgta actgcggcgg
     6961 atatccgagc ccatgtcgcc cacgcacatg ctgccggtgt tcagtccgat gcctatgcca
     7021 atttccggca agcccttgct gcggtgctct tcattgagct ggcgcacagc ctgcaccatc
     7081 tccagcgagg tcttgaccgc cagagcggcg tgctcgggcg tctcgacggg tgcaccccag
     7141 aaagccatca cgcagtcacc catgtacttg tcgatggtgc cgcgattgct gcggatcagg
     7201 tccgtgagcc ggctgaaaac actgttgagc agcgcctgca actgcgtcgg ttccatggtt
     7261 tctgacatcc gggtgaagcc acgcatgtca cagaacatca ccgtgagctc cttggccgat
     7321 gccttcatgc tgtaactgtc ggggtccttg accatttcat cgaccagctc gggtggcaca
     7381 taggtgccaa acaagttggc cagttcccgc ttggaacggc tttccacaaa atagccataa
     7441 ctcatgttca aggcaaacgc cgtgagcgcc atgaccagcg cggtggccag tggcaagacc
     7501 aggccatagg ccgaatatag ccagaaattc agcccggcca gggcggctgt caccgcggcg
     7561 ctggtcgcca ccgcgcgcgg ggcggacagc aaaggcaagg ccagagccag tgtcagcccc
     7621 gacagcacca gaatcaccac ctcgaaacca accgcatagt ccggtttgac aaagaccttg
     7681 ccatcaagaa accctgaaat cacattggca tgggtctcga cgccgggata ggtttccccg
     7741 accggcgtga ccctcaggtc cagcaagcca ggcgccgtgg tgccgagcaa gacaatcttg
     7801 tccttcaatt gacccggcgc catttttccg gccagcacat cggacgcgga gacataccgg
     7861 taggagccac cactggcgcc accaggcccg cgaaacggca ccagagtcgc cacccggtca
     7921 tccaccggga tggcgagcgt tttactgccc tgcttgagca aaatgctttc cagcccctgg
     7981 taattgcggg tcaggaattt ttcctgcgga aatccgggct cgacgccggg ctggcccacc
     8041 agcatgcgaa acatcgccag ggacagcgac tcgtagtact cgcctttgta ttcagccagc
     8101 aggggaatcg atcgaacgac cccgtcgcct tccgtgatgg agttgaaaaa ccctcccaac
     8161 ggcgcagcct tggcgatggt atcgatgttg gcaccgaagc cgttccagct ggtaaaccgg
     8221 atgggccgcc cctttagcgc ctcccgcccc atgacgggag caggcaaaac accgttgacc
     8281 cggccgtccc ggtcactggt gaagtaatac cccagcacga cgggtttgtt ctccagcgat
     8341 tttgcgaaga cggcatcgta gtcaaggctg gcctggatct ggccgagctt gtcggtaaag
     8401 gcgggctggt ccttcaattc gttccgggcc agttgctgca gccttttcag gccggagctg
     8461 tcgtcagcct ccgcaaaaac cacgtcgaat cccagcagag cggcctgttg ccgccccagc
     8521 aactcgtcca cgagcgcggc cagcttgttg cggccccatg gccagcggcc ggcttccgcc
     8581 aggctttttt catcgatgtc cacgatgaca atgcgctcat ccagcgtctt gggcatggtg
     8641 gcccgaaggc gggcgtcgta aagaatgtca tcaagccgct gcaggacatc caggcgtaac
     8701 acaccactgg cgtggagcaa ggcaaaaacc agcggaatca gcgtgacggc aatgcgaggc
     8761 caatgccggg aaaaaagtcg cataaggcct cttgaagagc cgcgatttgc agtggatttc
     8821 cggccgaacg cggggtatca gcctgatcag cgctggacga attgcatttg cgtgcctgcc
     8881 agctcaagta catcgccgtt tttcagcggc accggctcga cgccaatgtg cgctccattc
     8941 agcgtgggct tgttgtcccc ttccacatgc gccacgacaa agccatgcgg ccgcttggtg
     9001 atggccgcaa ccgccacccc gggcttgccg atggtggtga ccaccttgac cagggcaact
     9061 tcccggccag cggctgcgcc tgacatgacc ttgatggccg cgctggtgcc cgccccatcg
     9121 gcgggtgcag gcgctgccgc ggctgttgcg gccccggcag gcgcgaccaa cccggccgaa
     9181 ccggccttga tgatcatcgt tttctcaaag cccgcactcg cggcttcgtt gacatacttg
     9241 atcttgtact tgccgatttc aacggtgtca ccgttgttga gctgctgctt cttgacagcc
     9301 ttgccgttga gatacgtgcc attggtgctg ttcaggtcct caacaaaaac ctcgttgccg
     9361 gacatctgca gaacggcatg ctccccactg acggccaggt tgtcaatgac gatgtcgttg
     9421 taaggacggc gcccgagcga cgtgcggtcc ttggtcaact ggacctcttt gatcacaacg
     9481 ccgtcaatag agacgatcat tttcggcatg gccgactcct gtctattttt tagtatgtca
     9541 ctcaatcgga ctatttcccc agcatcctgg acagcaggcc gcgtttgacc gaggcctcct
     9601 tggcttgcgc cagcaatacc gaaatattat cccggccgcc gctgtcgttg gccgcagcaa
     9661 caagctgcct ggcgatctgc tccagcgact tcgatcccga tagaatagcc gcaatcgcct
     9721 cgtcactgat catgtcggac aggccatcag agcacatcag gtaaatatca cccagctcca
     9781 cacggtgttc gttgacttcc agcaacaccg cgtcctccac acccaaagcc cgggtcacca
     9841 gattcttgtt caccgaagtc agcgcctgct ccggtgtgat cagccccgcg tcgatctgct
     9901 cctgcagcag ggaatggtcc ttggtgatct gctcgagctg gccaccccgc aagcggtaac
     9961 agcgcgaatc gccaatgtgg cccagcatca ggcgggtgtt ctggaacaca ccgaccacca
    10021 gcgtggtgcc catgcccgag tactgcgggt tggagttggc tgcgttgaaa atggaatgat
    10081 tggcgttttc gacacagatc tccatcgccc gccgcacctc cttgccgttg gcatgcttac
    10141 cagcctgcgc cagccagcgc cccaattcgg acttgataaa tgccgtcgcc atgccgctgg
    10201 caatttcacc cgcgttgtag ccacccatgc cgtcagccag aatgcaaaga cgggtagggg
    10261 catcaaacgt cacggagtct tcgttgttgt cccgggttaa accggggtct gtctgggtgc
    10321 aaatctcgta aatcatcgtg tttttgttat ggcttcaaat ctgtagcatc aacgtcggcc
    10381 ggggaatccg gcagagagcg ggccgcaatt gtccgtgcaa aagcaggatt gtcgccgggc
    10441 gcaaacactg ccgtcttttc accgccttcc gaacctgtgg tggcggcacc ggcggggaca
    10501 gcagagcctg tgggcgcagc cgacagctcg gcaatggcga tatgcagatc agcggcaaac
    10561 tggtcgccat cctgataacg cgtttcaggc tttttgctca gcgacagcgc gacgatatcc
    10621 gccagccgcg cggacaactc atggcggatc acgcgaatgt cgggcgccgg ctcattggct
    10681 atcttgtaca tcagctcggc catggagtcg ccacggaaag gcaaaacgcc cgccagcatc
    10741 tggaacagca tcacacccag cgaatagaga tccgagcgtc catcaacctt cttgccggca
    10801 atctgttcgg gcgacatgaa actcggggtg ccgagcacca ggccggtttt ggttttgctc
    10861 gagtcggtga tacgcgcaat gccgaagtcg gtcactttga cggtgtcact gtcggggtcg
    10921 tacatgatgt tggccggctt gatgtcccgg tgcaccacgt tctgcttgtg agcgtaggcc
    10981 agcgcctctg caacacgcgc cacaattgac agcaccttgg caatcggcag cagttgcccg
    11041 tccttggtga cgtccagcag gtccttgccc ttgaggaact ccatcgcgat gtaagccagg
    11101 tcgtgctctt caccggcatc aaaaatggtc acgatgttct ggtgctgcag gcggcctgca
    11161 gtttcggcct cgcggaaaaa ccgttcacgg gcgtccgtca actcgtcacc ctcaaactcc
    11221 tggctcaagg ccatggtctt gatcgccacc acacgtccga tcttggggtc cttgcccagg
    11281 tacaccaccc ccatggcccc cttgcccagt tccttttcaa cctgataacg gcccagcatg
    11341 ggcttttcga caccaccgcc gtcgagcagc atggtgcccc cggggtgcgc tccgccacca
    11401 cccagaatga cggtttccga cagattcttc gcgcgattga gtttggactg caggtccttg
    11461 tggttcttgt tgtagctggc catgtattcg tacaccgcct gggccttgtt gaattgccgc
    11521 ttgcgctcga aatcaagcgc caggttgttc atgttgtcca tcagcgcgtc ggtgaaaggc
    11581 acgcgtcgga accggtcgaa agccatgtcc agttgcccct gcccctgcag ggccaggccc
    11641 atcatgcggt tggtttcggc cgactcttcg tcggacttga gcttgccggc ctcggtcatc
    11701 aggaaacgct tggtggtcag tgccaggtgg ccaatcacca ggagcgcggc cggaaacacc
    11761 agcttgagcc aggtggcagc cgccgacagc aagccgaact cgatcaccag cagcagcaca
    11821 aacagcagca gcgtgatgac ggcagccttg cctgcagaca ggcgcggcag gccggcaatc
    11881 aggtaggctg cgacaaccag caggactccc agcgtggccc agatgcccca ggcgggctgg
    11941 acaatgaagt gttcgctgag gatgctggaa gtgatgtggg caatcgtctc cgccggtgac
    12001 agtccgggcc cggccggtgt aggaaactgc gtgccgacgc cggcggcggt ggcaccaatg
    12061 atcacaatcc tgtcggtgta tttgctcgcc gggatcttgc cggacaacac gtcgtagaaa
    12121 gagtccaccg cgaacgccgg cctgccgtcc cggcccttgt agaactgcgg caacatcagg
    12181 gcggcctcat ccgtgcctat gcgcaatttg ccgatctgca ccgattcccc ggcattgagt
    12241 ttgatatcgg tcgcagcgag gttcaggctc ttgaccgcgg cgagcaatcc catggacgga
    12301 accgcctggc cgtcatagtt gatcagcagc ggctcctgcc gaaccgcgcc gtccacttcg
    12361 ggcagctggt tcaaatgggc aatgcctgcc gcgctgctgc cgatgatctc tatgggctgc
    12421 tggccccgaa tggcgggcag cgagaatccg cccggctcca ccaccgcgct tttgagggca
    12481 taggcgggca gggggtcggg cttaccttgg ggctcaccca gcacaaaaac cgagggcacc
    12541 agcacattgc cagccttggc catgctcgcc gccagcttgg cgtccgtatc caatgcgacc
    12601 tccgcttcgg caatcacctt gcccagttgc tcgctgaggc tgtcctgggg agcagcggga
    12661 tcggcaggaa caacaaccgg tccgagcgct tctttcatct tgcggatgaa caccagcccg
    12721 cggtcggtct gcggttcaaa aaagaaggcg gtatgcgcaa tggttttgac tttcgcagcc
    12781 gccagctggt caatcagctg ggcatgtaca tcgcgcggcc acggccagcg gccaatgttg
    12841 gcgatgctct ggtcgtcgat cgcaatgatg gcaatgcggt ccgacggctg gcgcgacgtg
    12901 ctggtactgg caaagtcgta ataccggcgc tccagcgtgg caaagaagtc ggtcgcgctg
    12961 tgcagcacca gcacggccaa caccaccaga acgcccacaa accagtcggc tcgccagaat
    13021 tgcccgcgct tgcttgatga tgtggccacg tccgtcccgc cttttgtcct ggttcgccag
    13081 accgcgcgcc agccgcgccg gcttcttttt tgcgcacttg tcacccggcc agcgttacag
    13141 gccggttaca aattcaccgc ctttggacat taacagacgc caagaacccg gacaagggac
    13201 ttttgcacac ggccagaggc ttgtgttgca aattggtgcc gctggcgccg gggctgcatg
    13261 cctggagccc ggcggtggcc gggactcagg ccgcgttggc cggcttttga cgtgcctgca
    13321 ggattttgcc cgctgccagc accaacaagg cgccggccac ggccgccgca tagctccaga
    13381 gcgccgaatc aggcagatag gccttgatcg ccgggtcggt ataagccatc tgaccggcaa
    13441 tccagcccag caacatggcg cccagggtga tcacgatcgg aaagcgcccc atcagcttga
    13501 gtaccagctg gctgccccag acaatgatgg gaatgctcac cagcagacca aaaatcacga
    13561 gcgccagttg atggtcctgg tgcgcccctt gggcggcgcc tgcgatggcc aggacgttgt
    13621 cgacgctcat caccaggtcg gcaatgatca cggttttgac cgcgccccag agcttgtcgc
    13681 tggagctgat gttgtggtcg ccttcgtctt ccggttgcag caacttcacg ccaatccaga
    13741 gcagcagcaa ggcgcccaca atcttgaggt agggcacggc cagcaatgca agcgcaaagg
    13801 caatcaggat cacgcgcagg acaatcgcgc ccatggtgcc gtaaataatg cccttggtgc
    13861 gttgcttggg aggcaggttc cggcatgcca gggcgatcac caccgcgttg tcgccaccca
    13921 gcaggatgtc gatcatgatg atctggccaa ccgcgagcca gaagccagcc gtcatgaact
    13981 cttccatgga aacctctcag aaaaatcaaa aaaccgattg tcagcgatga caaccggtct
    14041 gtctgttgtg gctaaccgca atacgcggta acttagagca gtgccttgag cagcttggcc
    14101 atttccgaag gattgcgcgt gatggtaaag ccgcactctt ccatgatggc cagcttggcg
    14161 tccgccgtat cggcaccacc ggagatcagc gcaccggcat ggcccatgcg cttgccggca
    14221 ggggccgtga cgccggcgat gaagccgacg atgggctttt tcatgttggc cttgcaccag
    14281 atggccgctt ccgcctcgtc cgggccgccg atttcgccga tcatgatgac ggcatcggtg
    14341 tccggatcgt cattgaaggc cttcattaca tcgatgtgtt tcaggccatt gatcggatcg
    14401 ccgccaatgc cgacggcgct ggactggccc aggccaattt cggtcagctg agccacggct
    14461 tcgtaagtca gcgtgccgga gcggctgacc acgccgatac ggcccttgcg gtgaatgtgg
    14521 ccgggcatga tgccgatctt gatttcttca ggtgtgatca ggccggggca gttggggccc
    14581 agcagcaggg ttttcttgcc gccggccgct tccttggcct tcatgcggtt gcgcacttcc
    14641 agcatgtcgc gcacggggat gccttcggtg atgcagatcg ccaggtcgag gttggcttcc
    14701 acggcttccc agatcgccgc ggcggcgccg gctggcggca cataaatcac cgacaccgtg
    14761 gcgccggtgg ccttggcggc ctcggtcaca ttggcaaaaa tcgggatgcc ttcgaagtct
    14821 tcgccggctt tcttcgggtt cacgccagcg acaaagcact ctttgccatt ggcatagtcg
    14881 cggcacatgc gcgtgtggaa ctgaccggtc ttgccagtga tgccctgcgt gatgactttg
    14941 gtgtttttgt tgatgtagat cgacatgttg gttctcctgg cttacttgac ggcggccacg
    15001 attttggtcg cagcttcagc catggtgtct gcggcaatga tgggcagacc ggagtccgcc
    15061 aacattttct tgcccaggtc ttcgttggtg cccttcatgc gcaccaccag cggaaccgag
    15121 aggttcacga ccttgcaggc ggtgatcaca ccgtcggcaa tcgtgtcgca cttcatgatg
    15181 ccgccgaaga tgttgaccag gatgcctttg acatcggggt tcttcagcat gatcttgaag
    15241 gcttcggtga ctttttcggc tgtggcgcca ccgccgacgt ccaggaagtt ggccggctcg
    15301 ccgccgaaca gcttgatggt gtccatggtc gccatggcca gaccggcgcc gttgaccagg
    15361 caaccgatgt tgccatccag ggagatgtag gcgaggtcga acttggacgc ctcgacttcg
    15421 gccgggtctt cttcgtccag atcacgcagg gccacgattt cggggtggcg gaacagcgcg
    15481 ttcgggtcaa agttgaactt ggcgtccagc gcgatgagct tgttcttgct gtcgcggttc
    15541 aggggattga tttccaccag cgatgcgtcg gtgtccatgt agcacttgta gagcttctgg
    15601 aacacgtcca cggcctgggc ggtggactct gccggcagcc cgatgccgtt ggcaatcttg
    15661 gtggcctgcg cgtcgctcag cccggtgagc gggtcaatga actcggtgat gatcttttcg
    15721 ggggtggagt gcgccacttc ctcgatgtcc atgccgcctt cgctcgacgc gatgaaggcc
    15781 accttctggg tggcgcggtc ggtcacgatg gaaacgtaat attctttctg gatgtcagcg
    15841 ccgtcttcaa tgtacaggcg gcggaccttc tggccttccg cgccagtctg gtgcgtgacc
    15901 agttgcatgc cgaggatctc gccggcgagt tttttcacgt cttcgatgct gcgcgccagc
    15961 ttgacgccgc cgcccttgcc gcgtccgccg gcatggatct gggccttcac cacccagacc
    16021 ggtccgccca gtttctgggc ggcttcgacg gcctcttgga cggtgaacgc aggaatgccg
    16081 cgcggcgtgg ggacaccaaa gttgcgcaag atttccttgc cttggtactc gtgaattttc
    16141 atgaaattac ttccaggagt cggtaaaaag gggcgttcgc cgaagtgatg tcaatctgcc
    16201 acatttgcgc agaattcacc aaaatgtaac gccttcggtc actgcacgca gcagcgcaga
    16261 actgtatcat gttgcgatgc acaattcttg tgcgcggctt acaccgccct tctaagcact
    16321 ttcagcagga aaaagccaca tggccaaggt ctttatcgac ggcgaagcag gaaccacggg
    16381 tttgcagatc cgggaacgcc tgcaagccat gcccgccatc gagctggtca gcatcgcgcc
    16441 cgagctgcgc aaggacgctg ctgccaagcg cgcgctgatg ggcacggtcg acctggtggt
    16501 gctgtgcctg cacgatgacg cggcacggga atcggtggcc atgatcgacg acctggcacg
    16561 cgtcagcggc aaaaaagggc cgaagatcat cgacgcctcc accgcgcacc gcaccgcgcc
    16621 gggctgggtg tttggcttcc ccgaactggc ggccggccag cgtgaagccg tggccaacgc
    16681 cgaccgcgtg gccaaccccg gctgttattc gaccggcgcg atcgccctga ttcgcccgct
    16741 ggtcgatgcc ggcctgctgc ccctggacta cccattggcg ttgcccgcag tcagcggtta
    16801 ctcaggcggt ggccgcacca tgattgaaga ctacgaagcc ggcaaggcgc cgccgtacca
    16861 gatctacggc ctgaacctca aacacaagca cctgccggaa atcatgcgct acaccgggct
    16921 gacacgccgg ccggtgttca cgccatccgt cggcaacttc aagcagggca tgctggtgca
    16981 gctaccactg cacctggatt tgctccccgg ccggccaacg gccgtgcagt tgcaggacgc
    17041 gctggcgcgc cactatgcgg gcagccaatg ggtcacggtc gagccggtgg gtgacagcac
    17101 ttcgatagac gcgctcgcgc tgaacgacac caacaagatg gaattgcggg tgttcggcaa
    17161 cgaggagttc cgccacgcgg tgctgattgc gcggctggac aacctcggca agggcgcttc
    17221 cggcgcagcc gtgcaaaacc tcgagctcat gctgggcctg taaggcgcta catattttgt
    17281 agctgttggc gcccgcgttg attgggctag aggccaattc cttgaaacag gggtggccgc
    17341 cgtcagtcgt cctcggcttg cgacaccaca cggcggatcg cctccgcccc aaaaccgcga
    17401 gccgccagaa aacgcatctg ctttccgcgg gctgcggcgt cggccgccgg ctcgccgaac
    17461 ttcctgcgcc agacctcgcg ggcgcgttcg acctcactgg ctttcaggct gctcacagct
    17521 tcggccacag cctcgggcgc cagtcccttg ccctgcagct catggcggat gcgcgccgca
    17581 ccaaaacgcg cggcgcgccg gttgatgacc gattccacca cccgcgcctc gctgatgaag
    17641 tccttggcct gcaggtcgtc cagcacctgg gccagttgct cgggcgactc ggcatgtgct
    17701 gccagcttgc gctcgagctc gctgcgcgag tgctcacgtt gcgcgagaca gcgcaaggcc
    17761 cggcctttga gggacaggcc gaaacccggt gtcgcgggag gtttgcctgg tgtcatgccg
    17821 acattccgtc gtcaactact actgttttga tagctacttg cgatttacag acaagggctg
    17881 gagcctgatt tggcataaaa cctggcatac cagccccgtc ttttcaggcg gccgcgactt
    17941 tggaggcttt ttcaaccttg tctgcttttt cggccttctc cgccttttcc ggcttgttgc
    18001 cgaccgcctc ctggatcaag gggatgccca gcgactcgcg caccttgttt tcgatttcca
    18061 tggccagctc cgggttttcc ttcaggaatt cacgcgagtt gtcgcggccc tggccgattt
    18121 tttcgccatt gaaggcgtac caggcaccag ctttctcgac aatgtggccg gccaccccga
    18181 gatccagcac ctcgcccagg cggctgatgc cttcaccgta aaggatgtcg aactccgcca
    18241 tcttgaacgg cgaggcgacc ttgttcttga ccaccttgac ccgggtttca ttgccgacga
    18301 cttcctcgcc cttcttgatc gagccgatac ggcggatgtc caggcgcacc gaggcgtaga
    18361 acttcagcgc att
//
LOCUS       DAIDFG010000023        18185 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_23_length_18185_cov_17.494595, whole genome shotgun sequence.
ACCESSION   DAIDFG010000023 DAIDFG010000000
VERSION     DAIDFG010000023.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 18185)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18185)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18185
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23_length_18185_cov_17.494595"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3458 a         5924 c         5686 g         3117 t
ORIGIN      
        1 gacgcaaccc tgcgcctggc agccagcata aatcacaaac accccgccgg tcaaaacacg
       61 gcgtccggaa cagttaccaa atgaagcgcc gaagcaccgc gcgcagcgct ttcgcaagtg
      121 ctgccggcca cagctgagca tcactcaggg gatgccgcat ttcgtgtcca tcaggagcga
      181 cctgcccgcg cgtcgtgatg ggccactggc tcggggcact catgctggag ggcgccgcga
      241 cgcagatgca ttttgtgaag caggatgctg gcgccggccg cagacggcag cagcaaagcg
      301 cataaaccca ggggttccgt caaatcattg aacacgcgcc cacgccctct ttcggggagt
      361 ctaaccaaat cccgggagaa agataacttt tctatctaaa acaatttatt tttatccaaa
      421 acacgccaat cccgtccaaa cggaccctgg tggaacccgg gccggtggta gtcagcggat
      481 tgcgccagtc gccggggcta gcggaatttt ttggtgaatt cgcgcacgac gccgatgatc
      541 tcggcagtgc ccaggggacg gatggggtag cgcacattga gcggcttgag gaacagctcg
      601 ccaccgtcct tgatgagctg cttgaaggtc gtctggttct tgccgttgag agcgatcaca
      661 tagtcgcccg gcacggcctg catttcgggc tccacaatca gcagggagcc ttcggggaag
      721 gagtcgaggc tgtcgccaac catgctgtcc ccctgaacac gcaacgcaaa ggtgtgccgc
      781 ttgatggcaa cggtcaccgg caccatttcc agcccgtcct tgggactgaa gttgtcaatg
      841 accgtgtaat ttccggcctg tacttcggag atcagaggca cttcagccct cacgtcaaag
      901 cccgggctga cgttggaacc gccagacatc aactcggcca cggaaacgcc aagcagctct
      961 gccaccctgc gctggttgga acgttttggc gcggtgccgc ctggtctttc ccattgttgt
     1021 acggccccgc ggctgacacc tgcagcgtca gcaaactgct gctcgctcat gcccagccgc
     1081 ttgcggcctt ccctgattag tgtgtgaacg ctcatagtct ataggttagg cctcggcctc
     1141 gatagaaaat ttgtctctcg atagataatt tagcgttcac gcggaacgca gcttcggtgc
     1201 tgattgaccg tgaaagggcc tgaaacaggc gctgcggcgg cgccttccaa acaagctctg
     1261 tgcgtagcct gttgttcttc ttcttgattc ttcttggtat tactgaagga tctgggtatg
     1321 aaattacata aaaaaaggtc ttcaaaaccc tgtcagtctt ttttattaat tgctgaaaag
     1381 tatgaaatgc caccccagaa tgccgcccaa gacaggacgc taactgtcct tgagcaaaaa
     1441 ctgcatgctg gttcccgcca gttccagcac gtcgttgtgg gccagcggca ccgcatcggc
     1501 ccctatcggc tggccattga gcgtcggcgg gacctcaccc gccatgtggg ccacgtagta
     1561 gccgtagcgc cggtgggaga cggccacgac gctgacatcg ggcttgccga aagtggtcac
     1621 cgccttgacc acaggcatct ccagtccggc cgaagagccc gagagcacct tgaagctggc
     1681 gtgcagcgcg ccgctccccc ccggcgcggc atggccatcg agcttcatcg ccgcggtctc
     1741 gtggtagccg gaatcctccg aagccgagag gtactggatc ctgaagtttc cgatggcgat
     1801 gatgtcgttg tcatgcagca attgccgtga cttgatcatc ttgccgttga tataggtgcc
     1861 gttggtgctg cccagatctt cgacatacac atcggccagg ccctccagtt caaatacgca
     1921 gtgctcgccg ctgaccacca ggttgttgaa cacaatgtcg ttgtgcggcc gccggcccag
     1981 cgtggtccgg tccttgtgca ggtagacatg cttgatttcg acaccctcga ccgatgcaat
     2041 caattgcggc ataaggcctc ccggctgcgt ccagactgcc gcggtgcgcc ctttgcgcaa
     2101 cggcctggcc atcgtatgcc tggcgctgcc aggaaacaag ccccccgggt cagcgcttgc
     2161 gcaagggttc cagcaggccg cccagcccgt tgtgatcgat ttccagcatc agcgccagca
     2221 gccgaccgat ttcattcctg ggaaaacctt cgcgggcaaa ccacgcgagg taattgcccg
     2281 gcaggtcggc aatcagccgc cccttgtatt tgccaaacgg catctcgcgc gtcaccagca
     2341 gttgcaggtc ttcaggcttc atgcgcccgc gcgcttgacg gtccagccct gtttggtgag
     2401 ccagtccacc accagcgcgg catggtcacc ctgcacttcg atcacgccgt ccttgaccgt
     2461 gccgccggaa ccgcaggccg tcttgagctg cttgccgacg agaacgagcg ccgcgtcctg
     2521 caaggccagc cccttgacca gggtcacgct tttgcccccg cggcccttgg tctcgcgcga
     2581 cacccggaca atgccgtcgg acgggggcaa gggccgcgtc tggcggcaca cgcactgggc
     2641 cagcgccttg cggcaagccg ggcacatgcg ccccgcgtcg gtcgaataaa cgagtccgcc
     2701 ggggcttgaa tgacttttca tggaattcac cgatgtgagg aaaacggcct cagagcccat
     2761 cgccccgagc gccaccggct atcgtacagc gatgcgggca ggctcccggc ttaccggcgc
     2821 atgctcagat gtgcccgaca ggctgcgggg tgtgccgcaa gtgaagagac aggcccagcg
     2881 cctccatgtg gttgccgtcg cggcccagaa tggtttcggc cagcggcagg aaatattcct
     2941 cctcgaaacg cgcatgcgag gaataggcgt gaaccagttc ctccacggca tccagatcca
     3001 ggtcgacaga ctgggacttg accacggcct tgacaacagg ctccagcttt ttccacatcg
     3061 cctcaatctg gcggtgctcc gcagtcaaac gttgcaccat ggcctggacc tccccggctt
     3121 cccggccccg gccggcgctg cgcaacacgg cgggaaacag ctcacgctcc tcgtctgcat
     3181 ggtgctcgta caccgcgtgg tggaacaggg ccagcgtgtc ggtggcgatg gttcgcgcct
     3241 gcgccgcagc cgcctggagc gcaggcaact cctcgaaggc ctgaagcttc gacagaatgc
     3301 ccagatggca ctgggaaaaa ccggtcagtg gctcgctgga atggcccggg tcgtgcgcgg
     3361 cagaatgggt tgtcatgccg ccaatatagg ggggcgtctg tgcgtccggc ttgatgcaca
     3421 tcaataaagc ggccgcccac ctccgcaaga taagggcctg ttcatcttca tcagacaagg
     3481 cccccatgtt caagaacatt cttctggcga ccgacggttc cgaggcatcg gcccacgcgg
     3541 cccaactcgc tgtcaatctg gcgcgcacac acggcgccaa actcaccgcc ctgtatgtcg
     3601 tggaccccta cccttacctg agcatcggcg agaccaaccc gctgggcgtt caggcctaca
     3661 tggctgcggc ccagcaacac gctgcgcagg cccatgccaa ggtcgcggcg ctgtgcggtg
     3721 cgccgggcgc ggggatccag ctcgaagcgc ggatggccga gaatgtgtcg gccgccagcg
     3781 gcattgtcca gacggtcaag gacgaaggcg cggacctcgt cgtcatgggc tcccacggcc
     3841 gcaccggcat cgcccgcctc atgctgggca gcgtggcctc caaggtggcg gccgagtcgc
     3901 cggtgccggt gctgatcgcg cgctaggcgg cttgcggctg cggccaccta ccagccgatg
     3961 gccttgggca gccacagcgc gatctgcgga tagaagaaca gcagcagcag cgccaggaac
     4021 tgcatggtga tgaagggaat cacgcccttg tagatgtcct tgatcgtcac ctcgggcggc
     4081 gccaccccct tgaggtagaa cagggcccag ccgaaaggcg gcgtcaggaa ggacgactgc
     4141 aggttcaccg tgatcagcat ggccagccag accgggtcca cgccctgggc cagcagcacc
     4201 ggcaggaaca ggggcacggc gatgtagctg atctcgatcc actcgatgaa gaagccgagc
     4261 acgaagatca ggaccatcat gaaccagatg tcgctgttca cgccgccggg cagcaggtcg
     4321 aacatcctgg tgatcaggtc ttcaccatgc aggccgcgga aggccagcgc aaacacctgc
     4381 gccgtcatca ggatcagcat catgatggcg gtgatcttgc tggtctccag cgccgcctcc
     4441 ttcaggacct tcagtgtgaa acggcccgag aagagggtga tcagcacggc gcccaccgcc
     4501 cccatggaag ctgcctcggt cggcgcggcg atgccaccga cgatggagcc cagcaccgcc
     4561 accaccagca tgatgggcgg caccacgact ttccagaagc ggatccagag ctggcggcgc
     4621 gagacgccgt cgcgctcggc ctgcgggatc ggcggcatca ggtccggctt gagccagccc
     4681 agcaccagca ggaagatgat gtagaccgcc gacagcagca ggccggggcc caccgcggcc
     4741 gcgaacagcg tgcccaccga gagctgcatg atgtccgaca gcaggatcag gatcaggctg
     4801 ggcggaatga tctgccccag cgtgcccgag gcgcagatcg cgccgcaggc aatgctcttg
     4861 ttgtaaccgc gcttgatcag cgtgggcagc gtcagcaggc ccagggtgat gacggtggcg
     4921 cccacgatgc cggtggtcgc gcccatgagc acgccgaaca ggatgatgcc cacgcccatg
     4981 ccgccgcgca cgccgcctgc gatgtggccc atgacgtcga gcaggtcgtc ggccaggcgc
     5041 gatttctcca gcatcacacc catgaagaca aagagcggaa tggccatcca ctggtagccg
     5101 gcgacgatgc cgtagaaacg cgccggcagc aggctgaaca ggccgtcgcc gaagcccagg
     5161 tagccgaaaa tgaaacccac ggtggccagt gtcgtggcca ccggcacgcc cagcatcagc
     5221 agggcaaaga agcccaccag catcaggatg gcataggtct gcggttcaaa catggccgcc
     5281 ctcctttgct tgcgcacgct cgtggaagag acggatcagc gccgccaccg tctgcagggc
     5341 caccaggcca taaccgatcg ggatcagcga cttgacgatc cagcgcagcg gaatgccgcc
     5401 cggatcgggc gagctctcct ggatggcgta ggcctggccc acgtaggcga tcgacacctt
     5461 gatgaagatc agcgccaccg ccagcagaag cagcagtgag atcacgtcaa cgatgaactt
     5521 cttgcgcgcc gtgtattgcg cgtagaacag gtccacccgc acgttgtcgc cccgctgcag
     5581 ggcgtaacac atgcccagca ggatgagcgc agccagcagg tgccactcca gttcctgggc
     5641 ccagaccgag ccgaagctga aggcgtagcg cagcaggaca ttgaccgcaa ccagtccgat
     5701 catggcaagg gccacccaga gggtggcccg cccaatgaca tcgatcagcg attcaatctt
     5761 ctccatcacg gaggtgatcg ctttgtttga catgcaatac ctttctcaag gacctcagca
     5821 aacccctgcc cgttcgggcg gagcttgccg aagtcccttt tattgttggc catgcgctta
     5881 gccgggctca gcgcaaacgg ggtggcgtga gccctcgctt aacctcgcac cttggtgtgg
     5941 tactgctctt cgctgacctc gacccagcgg tcgtacttgg ccttgaagcc catgtaggag
     6001 tcgtgcacct tcttgaccag cgggtccttg gccacggcct cggccagcac cttcgcggtc
     6061 tcggcgcgca atgccgcgat caccgaatcc ggcagcggct tggcaatgac cttctggttc
     6121 ttgaccaggt cttccatggc ctcggcgttg gccttctggc accagccttc actgatgacg
     6181 ttgcaggcgg ccgcggcgtt gctcacgatg gcctgcaggt ccttgggcag cttgtcccag
     6241 gcggccgtgt tgatcagcag ctcggacacg gtggccgact cgtgccagcc ggtggtgtag
     6301 tagtacttgg ccgccttctg caggcccagg cggcggtcct ggtaggggcc gacgaactcg
     6361 gccgcgtcga tcacgccgcg ctccagggcg gggaagatct cgccgccggg cagcaccttc
     6421 acgtccacgc ccaggttggc gtagaccttg ccggccaggc cggggatgcg catcttcagg
     6481 cccttgaggt cggccacgct cttgatctcc ttcttgaacc agcccgtcat ctgcacgccg
     6541 gtgttgccgc agggcatggc caccatgccg aagggtgcgt agacctcatt ccacaggtcg
     6601 atgccgccgc cgtcatacag ccagccgttc atgccctgga agttcaggcc gaagggcacg
     6661 gcggtgaagt actgggcggc aaaggtcttg ccggtccaga aatagctgtt ggcgtggttc
     6721 atctccaccg tgccggcacg cacggcatca aaaccctcga aggccggaat aagttcacca
     6781 gcgccgaaga cctggatctt cagtcggccg ccggacatgt cggcgatacg tttggccagg
     6841 tcggtcgcgc tgcccgggcc atccatgtag aagggcgagc ccttggggta ggcgctggtc
     6901 attttccagc tgaaattctg ctgggcgcgg gcgatcatgg gaaagccgag cgcggttccg
     6961 gcagcggcgg cgacggaggc tttgctgagc agtgaacgtc ttgtggaggt catgcgatat
     7021 tccttggagt agagaagctg atgagacagc acctgctgcg caaacgggat gattgcgcca
     7081 agggttggca tgcaaactac gtgccacgga aaagtacaag aacaagcgac ttttcagggt
     7141 gtagtggact cactacgacc tgtagacaag gcccggccgg tggttgtcaa ctccgatgcg
     7201 gtgctgtcct agcggcgccc gcgcagcagc ggcctgaaca tcagcaggcc cagggccggc
     7261 cccagtgcga gccagggcaa caccaccggc aagggccagg cctgcgtggc ccccacgaac
     7321 agttcgatgc tgagcaccga gatcgcaaag ccgatgcaat tggcaaacgt cagcacgctg
     7381 ccggccaccg caggcggcgc gttctgcgcg gtcagggtgg agaactgcgg cgagtcgccc
     7441 gacaccgtca ccccccagag cagcatccag gccatgaacc acggcagcgg cgcggccagc
     7501 atcaccggtg ccgccacgca gcaggcgccg ctgatggcca gctgcaactg cgccacgcgt
     7561 gcgctgccga aacgccgcac cagcaggccg ccacccacac agccggcgct gcctacggca
     7621 atgatccaga aggccgcaac actcacagac gtgcccgcca gccgggtcgc cacaatcagc
     7681 ggcaccagca caaaaaaagc atagagctcc cacatgtggc cgaagtagcc gaacaccgac
     7741 gcgcgcaggc gccggtcggt ccagatcagg cccagcgccc gtggcgtgat gcgcgcaccg
     7801 cgcaccagat gcgggctctc gggcaccagc caggccgttg ccacgccgcc cagcgccgcc
     7861 agcagcgaca cacccagcac caccgcctgc cagggcgagc cgcccccggc aaacgccggc
     7921 aggcccgcat caccggccag cgcgcgcagg ccgtggggca aggccgtgcc cagcaccagc
     7981 gcgcctatca gcacccccat caccgcgccc aggcgttcgc ggtaccagct cgccgcgatt
     8041 ttcatgccca ccgggtaaat gccggccagc agaaacccca cgccaaaacg cagcgccagc
     8101 agcaaggtga agtcaccctg caccgagacc gtggcggcat tgagcgccgc accgagcagc
     8161 gagcagatca ggaacacgcg cgagggcgca aagcggtcgg ccagcatcag caaggcaaac
     8221 accagcgtgc ccgccacaaa accgagttgc acggccgagg tcaacatgcc cacggcgccc
     8281 ggcggcaagc cccaggcccg ttgcaggtca ggcatgacgg cattgaccgc aaaccacagc
     8341 gaggtgccgg caaactgcga cagcacaatg accggcagga tacgccgggg aacggcgccc
     8401 gtcatcagcg gaacctgacg gtcaggccgg ccaacggcgc atcaaacgcc gccgtccagg
     8461 tctcgcccgg cgccaccggc caggcgtccg tccaggtgcc ggtggtcacc aggtcgcccg
     8521 gttgcagctc gggcgcaccg gggcagctgc gcagctcggt caggaaatgc tgcagcgcat
     8581 gcagcgggcc gtcgagcaca ttcgcgccca cgccctggtc catctgccgg ccgccgcagg
     8641 acagcgtgat gcgtgccgcc gccaggtcct gctccagcgc ctgcgcgtcg cgggccagcc
     8701 cggccaccgg cacacgcgta ccgaccagca ggcgcgcgtg cagcgccccg tcggccacgg
     8761 tgtcggccgc cacgaacttc cagccgggct ggtgcgactg cacgatctcg aaaccgggcg
     8821 cgacccacgc gatgcaggca aacaactcgt cgagtgaggc attcgccggc ggggtggcgg
     8881 cgataccgaa gaccgcttcc ggttccagcc gcggctggca ggtgcccgcc agggacacct
     8941 cgccctcccc ttcgcaatac gacaaggtgc tgtcccagac cgtgccccag atcggtccat
     9001 acacctggta gcgcggccag atgctgcggt tggtaaaacc caccttgtag ccgcgcggct
     9061 gttcaccgcg gttgatgcgc aactgccgca ccgccagcgc gcgctggtag gcctcgggca
     9121 cgtccaggcc cgaagcggcg ggccagaaac cggcatggtc gtagtggtga agaagttgct
     9181 cgggcgtcat ggcgtcgagc ttaacgccgc tggccggatt ccacctgcca aaggattaca
     9241 tgccaaaaca gcctccaacc caatagacac ggacgcaagc agctatcatt tttgatagtc
     9301 agcaaaagag gatggcgagc agggccgggt cttccaccag gttcagcatc gcgtagccgc
     9361 ccaggcggta ggtgatgtag acggcctgcg ccatcagctg ttcaagcgcc atcatcgctt
     9421 agcggcgacc actcacgcct tggtctgcgc ggccttgcgg tcggccacga ctttctgggc
     9481 cgagggccgc tcgccgatga gcttgacgta actcttgtag tccaccccgc cggccagcag
     9541 caggtcttcg ccaaacgcga tcttgctggc catgcccacc agcggcaggc tggcaaaggc
     9601 ggcgcagtca gcctgggtga aggtgtcgcc ggccacatag ggcgaaaact tcgccagctt
     9661 tttgaaggcg gcaatgttgg cttccagttg cttgcggatg cgggccaggt tgccttcgct
     9721 cagcggctcg gcgccgaaaa aggccgcacc atacagctgg cgcgcgacga tttccaggtg
     9781 ccagtcgatg aaggtcgtga gctcgcgcac cttggcggcg gcaaaggcat cggccggcag
     9841 cagcggcggc tgcgggtagg cggtctcgat gtagtccagg atggcctggc tttcgcaaag
     9901 cccgccctgc gccgtgcgga tgaagggaat cttgcccagc ggcgaggcgc ccagaatcgc
     9961 ctcgtccttg ctgtgcgtgg cggcgtgctc ttcggcaaaa ggcacacctt tttcaagcaa
    10021 cgcgagtttg accttgttgt agtagttgga aacgggaaag ccgcaaaggg taagcatcga
    10081 agagtctcct gagttgatgg cgccaagttt atcgccgccc cgcgaggcct gcgtgcaacg
    10141 ggggtgacag cctcgccgcc gccgggctcc cgagggcgcc ggggctgtct gtgtgacatc
    10201 tgccgccttc tcctccattt gaaggacgac aacgcgcagc gccagcgcga cgatttcccc
    10261 cccatccact tcattgactg gaaacaccat gagcaactac ttctgggacg aaacgcaaat
    10321 gaaggccgcc ggcgccaacc cgaccatcct ggcgatcggc gactcctggt tctggtaccc
    10381 cttcttcggg ggcagcctga ccaactacct gggccagatc gtcgcgccca aaagccacgt
    10441 catcctggcc aagggcatga acggtgccga agcctttgac tacgtggacg gcaaatacgc
    10501 cgacagcgtg cgcagcgcgc tcaagctgta cggcgcgggg ctctcggccg tcttcctcag
    10561 cgggggcggc aacgacttcg ccggcttcaa cgacatgcgg ccgctgctca aggacagctg
    10621 ctcgggcgaa accacggcca acggctgctt tcgcggcggg gcgcagggcc tgccggggtt
    10681 tctggagcgc atggacacct actaccgcaa gctgatcggt gtggtctaca cccgcaccac
    10741 cctggactgc ctgatcgtca tgcacaccta cgactacgcg atcccggacg gcaagggtgt
    10801 gttcggcaag gacggctggc tgcaacccgc gctgacagcc gccggcgtca agcccgagct
    10861 gcaccaggcc tgcgtggtgc acctgctgga cgcgttctac aaggtgctgg acaacatcac
    10921 gaaaatggac cccgcccacc tgcacgtggt ggacagccgc ggcacgctgg gccccggcga
    10981 ctgggccaac gagttgcacc ccaagggctc gggcttcaag aaaatagcca cgcagtgctg
    11041 gaaacccgcg ctgaaaaaca tcgggctggc ctgagcggcc ccaagctgct tgagcctgcc
    11101 tggctacggc cggtcgggaa acagcggcgt cagcaggcgc acggcaatgt tgaagccgtc
    11161 gccggcaggc gtcacgcgca ccacctgcgc ctcgcaggtc agcgggtgca cccggcccgc
    11221 accgggaaac tccagcacca gttccatgct ggcgccaact tcatagctgc ggtccgactg
    11281 gaagtacagc ccggtcgcac tcaggtcatg ggtggcgccc tcgtcgccct cgatggtcag
    11341 gcgcaaggcc acgccaaaac gctgtgcggc ccgttgttca agcaacgtgg ccttctgttc
    11401 gccaccggcg gttgtttgca tggtcctgtc cctgaagatg cccgaaaacg ggcgtagctg
    11461 ccactttagg ccatgagcgc gccccgtgtg taggcacgcc cgcaggccgc gtgtgggaca
    11521 gcggacccaa tgcgccaccc cgaccgccag cattgaagat cagattagcc tccagcccaa
    11581 tactgacggg cgccaccagc tatcagcttg atagcaagtt gagaccggac ggatgaccct
    11641 atctgcaaag ctggtcctcg cagggcacgc cgtcgtggtc gccgtccatc ttcgtgccgg
    11701 ggcagttttt caggaagtac gtggcttcgg cgcaggaggt catctgcgag caatgctgcc
    11761 ggccgtcaca gacataggaa ggcgtcgcgg cgcgcgaagg cgccagatcg agcaagggtt
    11821 ggcttgcagg ggaagccgct tcactggcgc gaccggccgg ggccgccttc tggtaatacg
    11881 catacccctg ccaacccagc gccgccacca gcagcaggcc caccaccgac ttcagaccgc
    11941 tgccgggcga gttgcccgaa cgactgaccc gcacggggcg atggctgcgg tcgggctggt
    12001 acgcggcctg ctgcgcacca acaccctgca cattcaccgc acgctttttt ccctgagggt
    12061 tgagcgccac ctcgaaggtc aacgcctcgt tcagcaccgg ccggccaccg cgccgatatt
    12121 cggaaatgtg gacaaagatg tcctgcccgc cctggctggg gctgatgaag ccaaaaccct
    12181 tgtcgtcgtt ccagcttttg agggtgcctt cgaagcgcat gggcgagacc ttagggtgtt
    12241 cgattcatca atgtgggatg acagtgcaga cgaaagattt actgatcctg gagtcattca
    12301 acaaacaggg agtccaactg atcatcgttc gagcctgtgc cggcgccccg aaacggtcag
    12361 ttctgagaac tggaatgttg ttatttcgac tggctaacgc taggcggcgg agccaaatgt
    12421 ggtggcgatg gcgtagtagc tgctgccgca ggagtgtgcc cagccacagg ctcgctcatt
    12481 gaaacagatg gagtgccttt gagattgctc gatgcgatta tccaaatcga tagcgctgca
    12541 atcacgataa gggcaagtgc gagccacctt gccgcacgat aggcaaattc ccctttttta
    12601 acccgctccc attccgcttt taatacctcg cgcgcttgct tagcagcgat ctcaattccg
    12661 gacagtacct tgtcaacgtc gtctgtagtg ccgctaaagt actcgctcac aaaggtggag
    12721 ctttctgtca tgagagactc cagcttgatg tgaggctttt tgatttcgtt cagtcgaagt
    12781 ttaattcgat aggagcattc agcgacttgg tgacgcagtc tggccgttgt tgcttcgtcg
    12841 atgggcatgg gattcttaaa ctccagccta ctttcctgaa tagcccgagt acaggtcctg
    12901 ctggccgaga agaagtttgc taggtcttcc cgtaaaccat caatccaagc ttgccgaaat
    12961 tcggatatct tgttttcctt gttgactatc agagtaagcg cagcaatcgc gccagccata
    13021 aacgcggctg cgagcgtcac catcggcact agtgcggcag ggttgtattc cattctcgtc
    13081 ccctcaaaat cttactggca agatcggcga agtatgtgcc agcggtgaat cgctctggcg
    13141 cggtctgcca gttcagcgcc ggcagaaggc cgaatatttg gagcgcggta aatagtccag
    13201 caacggcgcg ggtggtacac agtatcgcgg tcaataatca gtggcagtga cgatacccag
    13261 tcttacggtc aacgtggcag ccgtctttgt cggtgccgcc tgagtgggcc agcaccaagc
    13321 cagcggccag caacagggca atcgcaagaa tttttttcat tggtgtctcc tgaaaagtcc
    13381 acgggccgcg tgggcgcggt tatttccctt ttaccaaagc gtactgttct tccattcgga
    13441 cgtagtagac gtcgcccgtc caccggttct gcatataggc agcgccaatg ctctctcccc
    13501 catcaatcgg cgtgatctcc catcggaaca tccagccaag gccaaacaga agtaccaaga
    13561 ccactgtcgc tatcaatttc atggtcagtc tggcgttggc tgctttattg gtttcatatg
    13621 ccattcgcac ttcgtgatca tgctgggacc aaccgccagg acccgggacc tgaggcaaag
    13681 aagacatcga cttttcattc acagttttct cctttcagct tgggcagcac aactcgtagc
    13741 ttcatccccc gtttagataa ggcgaggacc acaaatacaa cagacgccaa gtatgaagct
    13801 cggcttacag ggcgtcatcc tgcaatcaga ctagaccgcc ccattcttct tcatggcgcg
    13861 gcaacactca agccgatcca gtcatctgaa aacagcccct aagcctgcca aaccccatac
    13921 cccaactcac gcagcgcaat ccccaactcc acctccatgt cgcgcgcgcc ctcatacggc
    13981 atggggttgt acatggcgta caggccgggc agcaggcgca ccccccactt ctgcacatac
    14041 acgttggact ggatgccggc cttgtgtttg tcaaaacgca catcggggtc cagcccggtc
    14101 atgccgacgt acacaaacgg tttgccgagc tggtagtcgg ggttggcgcg gcgaaagcgc
    14161 cccacgttcc agacctcgtc ggacagctcg atcacgtaga cgtggtagtg gtggctgcgt
    14221 ggtgtgcgcg gcggtttttt ggcgcgcggc ttggccgggg cggcgtcggc cgcatcgccc
    14281 gctacggccg gcgggctttt ctttcggggg gtctttcggg gcgcggtagc catggtggct
    14341 gaagtatgcg gcaagcggcc gggtgcccgg ggcgctacca aataaatagc tgatcgcgcc
    14401 cgtgtatcaa gggatggagg ctgattttgt tgcaagaaac cggcgaatgg gcctccacga
    14461 atcaagtccg gtacggccct tcagcgggtt ccgagtgcca gtttttcttc ttcagctgtt
    14521 tcgctgcgcg tcgcgccggc gcagcgccca ggcgaagaac acaccctcgg ccgccatgac
    14581 ggcgccgccc agcgccacca ccgcaccggt gccgatttgc gcaatcaggc cggcggcgac
    14641 caccacacca atcgactggc ccaggaacag gaaggaagcg aacagcgaga ccgccgtgcc
    14701 gcgcgcgtgc ggcgccatct gcgtggcgtt ggcctgcatg gtgttgtgga acatgaaaaa
    14761 accaaacccg gccagcaggc tggcaggcac ggcgggcagc cagtgcggcg tgaaagccag
    14821 caccagcgcg cacaccccca ccaggatgcc gccgagcagc accagctttt gctggccaaa
    14881 gcggcgaatc atgtgacgcc ccaccgccat gtacatcacg ccgcccaggc cgaacagcgc
    14941 cacgatggtg ccagccaatg acagcgacag ccccagcgag cggtgcaggt gcgaggccca
    15001 gatagccagc acgccaaaac ccgccgcccc ttcgaccagc gccatcagca gcaccacacg
    15061 cgaccacggg ccggtgatga tgagccgcgc ctgtttcaca aaaccgggcc gcgcgccctg
    15121 cggcgccgcg gctgccaccg ccggttcagc cggctcttca gccggggccg cctgctgccg
    15181 gcgccagtcg ccccacagca gcaagcccac cacgccgaac agcagcgtca gaaaggcaaa
    15241 agcccagcgc cagccgagtg cgtccgtcag caggccgccg gcgagctggc cgccgacaat
    15301 gcccagcgtg ctgcccaggc cggtgcgcgt gagcatttcc tgcagctggt cggacggcac
    15361 ggtgtcgccg acccaggcca tggtcagcgg aatcagcgcg gccgcgccca gcgccatcag
    15421 caggcgggcc cataccagca tgtccaggct ggtggccagc gtggccgcga cgctgcccac
    15481 gcagcaaccc agcgtggcaa aggtgacgat gcggaacttg ccgaagcggt cgcccagcgg
    15541 gccatagaaa agctgcgcca cgccgtaggt caccgcaaac accgacacca ccagcgccgc
    15601 ctgccccagg ctgacggcaa acacgcgcga cagctcgggc agcatggcgt cgcagatgcg
    15661 ctgcgcggcc atgctggcaa aggtggccag cgacagcagc acgatggagc ggcgcgtggc
    15721 cgcggacaaa ggggcggaaa caggggcaga aacgggagtg gtggtcacga agcgaagggg
    15781 ttggttgacg cgctgcaagc gtcatgggcc aggcgccggc ccattgtagg caggacccgg
    15841 cctgcgcgct tggggcgcgg gcgccctgct attcattcaa gcgccacatc cgcttctccg
    15901 ttcgccctga gcctgtcgaa ggatcgaagg gcccttcgtc aagctcagga caggcttcga
    15961 caggctcagc ccgaacggtg aggcgtcttg acggtgtggc gtcttgagaa ctacggaaac
    16021 atcactaacg aacctggccg ggcggcaagg gggtgtactg cggcgcgcgt tcaccctgcg
    16081 gcaggccgat caccacgccg ggctgcgccg caccgccgct acccgacatc gggcgggcgc
    16141 cgggaatcgg ctcccagcgc gggtcgccgc ccggcggggg tggcgcggtg cccggtggcg
    16201 gactgtaaac aggcgggtta taggacggtt gcgggttggc ctgcggcggc ggcggggtgt
    16261 agctaggcat ggcctgctct tcggccggcg gctgccgggg cgtgaccggc tgcgacggag
    16321 acgggaacag cccgcgcagc cagtcgtgca cctggtcaac caccggctcg atcaccggct
    16381 cggaccggcc actgccgcgc ggcgcatcga aacgcgcgtt ggtgtccacc acccggctgc
    16441 gcagcgcctg ctggaaaaaa tcgcccacga tgggcaacgc gctgtgggcg ccctgacccc
    16501 agtagtcgct gagcgtcacg cggttgtcgt tgaagccgac ccaggcgccc gccaccagtt
    16561 gtgaatgcat cagcacaaac cagccgtctg tgttgtcctg cgtggtgccg gttttgccgg
    16621 ccacgtctgc ctggatgccg aagcgcgagc ggatgccggc accggtgccg ctgtcgatca
    16681 cgccgcgcat cacgtccagc agcacctgtg ctgatggaat ggacagggcc tgttcgggca
    16741 ccgcggtatg gaaggtttgc agcaccttgc cattgcggtc ttccacgctg gtgatcaaga
    16801 gcggctcgat gtagctgccg ctgttggcaa tggtgctgta ggccgacacc atttccttca
    16861 gggtcaccgg gctggtgccc agcgccagtg agggcaccac gtcgagcttg ctttgccgca
    16921 cccccatggc ctgcgccagg ctggccacct tggccgggcc cacgctctgc atgacctggg
    16981 cggtgatggt gttcttggaa tacaccaggc cgttgcgcag gctcatgggc gcgtagctgg
    17041 gcggggtgcc gtcgcggggc cgccagacgt tgccgttgcc cagcggaatc tccacgtcgc
    17101 cgtccatgaa gctgtcggtc ggccgggcgc cgttttcaaa cgcggcgccg tacacaaagg
    17161 gcttgaaggt cgaacccggc tggcggcgcg cctgctgcac gtggtcgaat gggtcctgca
    17221 caaagttgcg gctgcccacc cacgcgcgca cctggccgtt gcgcgggtcc atggccaaaa
    17281 aaccggcctg cagccgcgtc ttgtcctggc gcagcgccag catgaaatcc ttgtcggcct
    17341 gcagctgctt gagggcctct tcgtcggtct ggccggactc gcgggcggcc ttgtactcgg
    17401 cgctttcgcg cacaaaggct ttcacgatgt cttttttggc ggcccaggta cgcggccccc
    17461 attcgccgtt ggccacgccc tgcagcttgt cggcctggcg gatcagcgcc tggttggcct
    17521 gggcctgcag ccgcgaatcg atggtggtgc gcaccaccag cccgtcggca tggatgttgt
    17581 agtcgttgcg gtcggcccat tcgatcagcc agcggcgcac aaactgggaa aagtgcggcg
    17641 cggggcccag cggctcggtc tgccgctcga agtccacctt gagcggcctt ttcttcaggg
    17701 cctggaactt cgcctccggc agcttgccgt gtttgaccat ctgggacagc acgatgttgc
    17761 ggcgctgcgt ggcgcgctcg gggttgagca ccgggttgta ataactggtg cccttgagca
    17821 tgccgatcag cgtggcgctt tccagcacgt cgagcttgtc ggccgacttg tcgaaatagg
    17881 tgcgcgcagc catttcaatg ccgtaggcgt tgtacaggaa aggcacggtg ttgaggtagg
    17941 tctcgaggat ctcgtccttg gtgtagaccg cctcgatctt cagcgcggtg atggcctcct
    18001 tgatcttgcg cgtgaccgtg gccgagcggc cgatctcctt ggggtacatg ttgcgggcca
    18061 gctgctgcgt gatggtggag ccgccctgca ggtcaccggt gaaggtgtgg aacacggccg
    18121 aggccgtgcg cttgaggtcg atgccgtggt gctcgtagaa gcggtggtct tcggtggcaa
    18181 tcagc
//
LOCUS       DAIDFG010000024        18090 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_24_length_18090_cov_18.370668, whole genome shotgun sequence.
ACCESSION   DAIDFG010000024 DAIDFG010000000
VERSION     DAIDFG010000024.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 18090)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18090)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18090
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24_length_18090_cov_18.370668"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3352 a         5880 c         5835 g         3023 t
ORIGIN      
        1 tggctcagcc gcccaggctg agcgtcgtcg cgcgcctgga ttggcgctgt tttaaccacg
       61 ttcgcacttg catcaatagt gtgagcaggc ccttgccatg agcaccgaca aaaaaacact
      121 gctgctggtg gatggctcca gctatctcta ccgcgctttc catgcgatgc ccgacctgcg
      181 cgccgacccc ggcaacccgc aaagcccggc cacgggcgcc attcgcggca tggtcaacat
      241 gatgcagaag ctgcgcaagg acgtacgtgc cgactttgcc gcctgtgtct tcgacgccaa
      301 gggcccgacc tttcgcgatg cgctttaccc cgactacaag gcccagcgtt cacccatgcc
      361 cgacgacctg cgcagccagg tcgagcccat ccacgaggtg gtgcgcctgc ttggctggaa
      421 ggtgctggat gtgcccgggg tggaagctga cgacgtgatc ggcacgctgg cctgcatggc
      481 gtcgcagcag ggcatcgagg tcatcatctc cagcggcgac aaggatctga gccagctggt
      541 cgatgaaaac atcacggtga tcgacaccat gaacgaccgc cggcgcgatc tggctggtgt
      601 ggagtccgag ttcggcgtgc cgccgcgcct gatggtggac taccagaccc tggtgggcga
      661 cgcggtcgac aatgtgcccg gcgtgcccaa ggtcggcccc aagacggccg tgaagtggct
      721 gctcgaatac ggttcgctcg acgcgctggt ggcacgcgcc ggcgagatca agggcgtggt
      781 cggcgacaac ctgcgccaga ccctggactg gctcccaaag ggccgtgagc tgctgaccat
      841 caagaaagac tgcgaactgc aggactacat cgaaggcttg cctgctatgg aatccatagc
      901 tgtcggcgcc cagcagatgg aggccttgaa ggatttttat gacaagtacg gcttcaaggg
      961 cctggttcgc agcatagacg cccagaatgc gccggccgag ctgccggccg gacccgacac
     1021 ccaccgccag ggccgtgcgg cggcctcgtc cgacgagccg ggactttttg acgagccgga
     1081 ctttctggcc accccgctgg ccgagcgggc gaccaacctg cgttacgaca ccgtgctgga
     1141 ctgggcgatg ttcgagacct ggctggcaaa aatagaagcc gccgaactgg tggcggtcga
     1201 caccgaaacc acctcgctcg acgaaatgct ggcccagatt gtcggcctga gtttcagcgt
     1261 cacgccgggc gaggccgcct acattccgct gacccacgac taccccgacg cgccggcgca
     1321 gctgtcgcgg gacgaagtgc tggcgcgcct gaaaccctgg ctggagaacc cggccaaagc
     1381 caagctcggc caacacatca agtacgaccg ccacgtgttt gccaaccacg gcatcgaggt
     1441 ccagggttac gcccacgaca ccatgctgca gagttatgtg ctggaagtgc acaagccgca
     1501 cggcctggcc agcctggccg agcgccatgt gggccgcagc ggcatcaact acgaagatct
     1561 gtgcggcaag ggcgcgcacc agatcaagtt caaccaggtc gacatcgcca aggccgccga
     1621 atactcgtgc gaagactcgg accagacgct ggacgtgcac cgcgtgctct ggccgcggct
     1681 gcaggccgac gacaagctgc gtttcatcta cgagctcgag atgaaaagca gcgaagcctt
     1741 gtaccggatc gagcgcaacg gggtgctgat cgacgcgccc atgctggcca cgcaaagcca
     1801 cgaactgggc cagcgcatcc tgcaactcga ggccgaggcc tacgagattg ccggccagcc
     1861 cttcaacctg ggcagcccca agcagctagg cgagattttt ttcgacaagc tgggcatgcc
     1921 ggtgatcaag aaaacgccga gcggcgcacg cagcaccgac gaggaagtgc tggaaaaact
     1981 cgccgaggat tacccgctgc cagccaagct gctggagcac cgctcgctga gcaaactcaa
     2041 gggcacctac accgacaagc tggcccagct cgcgctgccg cgtaccggca gggtgcacac
     2101 gcactacgcg caggccgtcg ccgtgaccgg acgcctgtcc agcaacgacc ccaacctgca
     2161 gaacatcccg gtgcgcacgc cggagggccg gcgcgtgcgc gaggcctttg tggcgccgcc
     2221 cggttgcctg atcgccagcg ccgactactc gcaaatcgag ctgcgcatca tggcccacct
     2281 gagcggcgac gaagcgttgc tgcgcgcctt cactgatggc ctggacgtgc accgcgcgac
     2341 cgccgccgag gtgttcggtg tggaactcga tcaggtcagc agcgagcagc gccgctacgc
     2401 caaggtcatc aacttcggcc tgatttacgg catgagcagc ttcggcctgg cgcgcaacct
     2461 gggcatagag accaaggccg cggccgctta catcgacaag tatttccagc gttacccggg
     2521 ggtgaagcga tatatggatg aaacccgggt tttcgccaag aagcacggtt atgtagaaac
     2581 cgtcctgggg cggcgcttgc aactcccagg aattgcaaac gaaaaaggcc cgcgtctggc
     2641 tggtctggaa aggcaggcca tcaacgcgcc catgcagggc acggcggccg acctgatcaa
     2701 gctcagcatg gtcaaggtgc aggacgtgct cgacgctgaa aagcgcgcca ccaaaatgat
     2761 catgcaggtg cacgacgaac tggtgttcga ggtgcccgag gccgaggtcg aatgggtgcg
     2821 ccacgaaatt ccgcgcctga tggccggggt ggcggacctc aaggtgccgc tgctggccga
     2881 gatcggcatc ggtccgaact gggaaaaggc gcactgaatc cgcaggcagg ggcggcatga
     2941 ccgcggtggg acggggggaa taccccctgg taattccccg caccggcaag aacttccatg
     3001 accgcctaga ctccgactgc ttaatcagcc cagcctcttt caacgtccct ttcacgtccc
     3061 ttcaacccag atattgagga aaccgccatg ctcaacagcg accagaaatc cgagtttgat
     3121 tccctgctcc ccggccacag caccgaagcc ggtgcgaccc gccgcgcggc gctgaaggcc
     3181 gcgctcggtg tcggttatgc cgccacagcc ctgcccatca tggcgcagac cgcgatcaag
     3241 acgtcggccg agggcctgaa ggccggcgaa acctcgtttg aagtcaacgg gttcaaggtg
     3301 ccggcctatt acgccgcgcc cgccggtaaa accggcctgc ccgtgatcct ggtgatccag
     3361 gaaatcttcg gtgtgcatga atacattgcc gacaccgtcc gccgctttgc caaggccggt
     3421 tacctggcga ttgcgcccga gctgtatgcc cgtcagggcg accccacgca gtatggcgaa
     3481 atggccaagc tgatggccga ggtcgtctcc aaggtgcctg acgcccaggt catgggcgac
     3541 ctggacggcg cggtgaagtg ggcgggtgcc aacggcggcg ataccgccaa ggtgggcatc
     3601 accggctttt gttggggtgg ccgcatcacc tggctgtaca gcgcgcacaa ccccggcgtc
     3661 aaggccggcg tggcctggta cggccgcctg gtcggtgcca gcacgccgct cacgcccaaa
     3721 cacccggtcg acatcgctcc catcctcaac ggcccggtgc tcggcctgta tggcgggcag
     3781 gacggcggca tcccccttga cacggttgat aagatgaaag cggcccttgc cggcggcagt
     3841 cccgcggcga aggcctcgca gtttgtggtg taccctgatg ccccgcacgc tttccacgcc
     3901 gactaccgcc cgagcttccg caaggagccg gcagaagacg gctggaaacg cgctctggcg
     3961 tggttcaagg ccaacggcgt cgcttaaatt caagcccgcc cggcatggag ccgccccaca
     4021 gcaatgtgcg gcggcttttt tgcgtttcag gcgagttttt acaacctttt cgggcttcag
     4081 cccatataga tcgggcgtga gcagctatga cattgatagc aattttgacc ggcaccctgg
     4141 ccgccggcat aggcagcgtg tggctggcgg cgctgctcag ttttggggtg ctggcccgct
     4201 acaccaacca catgctcagc ctggccgccg gtgcgctgct ggcgactgcc ttcatgcacc
     4261 tgctgcccga ggcgttcgag agtcaggccg gcgcgcagga gctgtttgcc accttgctgg
     4321 tcgggctggt gtttttcttc ctgctcgaca aggccgagct gtatcaccac ggccatgagc
     4381 accatgaccc gcagggcggg gacgatgcgg gccatccgca tcatcaccat cacggccacg
     4441 atcacggcca gcccgtcagg tccggcagct gggccgtgct gaccggcgac agcgtgcatg
     4501 cctttggtga cggcatcctg attgcctccg ccttcgtcgc cgacatgcgc ctgggcgtga
     4561 tcaccgcgct ggccgtgctg gcgcatgagg tgccacacca catgggtgac ctggtggtgc
     4621 tgcgccatac ctccggcaac cgcagcatgg ccctgatcaa ggtgtcgctg gccggcgccg
     4681 tgacggccct gggcggcctg gtcggttacg ccctggtcga catgctgcac gactggctgc
     4741 cctatttcct ggtggtggcc tccagcagct ttgtgtatgt ggcgctggcc gacctgattc
     4801 cgcagctgca aaaacggctg acagcccgcg aaacgcttgc gcagattgcc tggctgggcg
     4861 tgggaatggc gctggtcatg ggggtcagcg ggctggcgca ttcctcgcat tgagcagctg
     4921 gaggccggcc atgtcagccg gacactgaca gacacagggc atggcagcct ataggcgcgg
     4981 cgaccggggg ccgccatcat ggaggcattc ttcgaggacg ctgccatgaa cctgctgacc
     5041 cccacccctt tcaaaccccg ccgggtccgc cgcattgctg cggcttacat cgcctacttc
     5101 accagcgtct gcgccagcat ggctgcggcg gctgccgtgg tgaccgagac cgtgccgctg
     5161 acgctgggag ggttctgaga tgcatgccga accgaaggtc attcccgaag aagaagcgcc
     5221 agaagagccg gcggtgacgc cgggcgacga cagcaacgac gaccccacgc cgccgctgcc
     5281 ggtggacccg agcggcacgc cgcagcgcaa tccggcggtg gacagggcgg ccaatgacac
     5341 cgaacctgca ctgccgacgg aagagcccga gatcggcaac gagcgcgaca ttccgtccga
     5401 cggtcgcgac ctggaaggcg agggcatgat tcgcgagctc gacaagccgg tggagccttc
     5461 ccccgccgct aaaccggaaa gggagtaaga acttgtccga acaaatgcca ggcatggcga
     5521 ccgaccccaa cgtgccgccg cccgaaccgg tgcagcccat cgccaccccg cccaaccccg
     5581 gccaggtgca gcccacaccg caacccatgt aacacggacg cgcgcaggct gtcagagccc
     5641 ggcttgcgcc agttgcgcgc gggtgggcag gccttcgctg tcgcccgcca cctgcacggc
     5701 gcgcgcccct atccaggcgc cgcgccggac ggcctccgcc acgctgcgtc cctccagcag
     5761 cgcgctgatc accccgaccg caaagccgtc accggcgccg accgtgtcca ccaccttgtc
     5821 caccggacaa gccggcacat ggccggtgcc ggcactggca ctgtcgaacc aggcgccgtc
     5881 ggcgcccagc ttcaccacca ccagcgcagc gccccgccgg cgataaaagg cggcaatgcc
     5941 ttcggccgtg ttttcgccgg tcaggaaacg gccctcttcg aggccaggca acacccagtc
     6001 ggcccgcgtg gccaggtcgt tgatggcctg gcgcatctgc ccgggcgtgg cccacaacgt
     6061 cggtcgcaga ttgggatcga acgacacgct gcgtccggcg ctgcgcatca gttccatgct
     6121 gcggtgtgcg gcggggaggg tggtcgccga aagggcggga aacacgccgg tcgcatggag
     6181 gtggcgcgcc gatagaagcc aggcttcatc gatgtcgctg agggccatgt gactggcggc
     6241 cgagccctgg cggtggtatt cgaccggcgg gtcgctgccg tccagcacac ggcccttgat
     6301 catgaaaccg gtgggctgcg ccgccgtgag caccacctgc gagcagtcga tgccttcggc
     6361 cgacatcgcc cggatcaggt agcgacccat catgtcggtg cccaggcggc tggcccagcc
     6421 gaccttcagc cccaggcgtg ccaggccgat cgccacattg gtttcagcgc ctgccgtgtg
     6481 tttgtaaaaa gaggcggcgt cttcgagcgg gcccggccgg tcggccacca gcagcatcat
     6541 ggcttcgcca aaagttacga catccagcgc gctggtcatg ggtgtttacc gggagagtgc
     6601 agggtgcgcg aatcgtagca cctccagttt gctattttat tgatagctgt tggcgcttgt
     6661 cggacatggg ctggagaacg atttgatgcg gcggccggac caaccgcaac ggcttgtcac
     6721 tttcgtgctg tcctacaaaa acatcaacgg cgtctgacac aggcgcccgg gagcgctgcc
     6781 tagagtcgtt ttgtgtctgg ccctccgcca tgcatgcagc accaccggcg ctgtgaatga
     6841 ccaacccaac catcaccagg aaacccacca tgagcaaagc acctcccaag atgaatgacg
     6901 ccgtcgacct gctcgatgcc gaccacgttg ccgtcaagaa gctgttcacc cagttcaaaa
     6961 aactcagcga agccaaggcg cccgccgacg agcgcaaggc gattgccgac aggatctgca
     7021 aggagctcac cgtgcatgcc gacatcgaag agcagatttt ttaccccgcg gcgcgcgagg
     7081 ccatcggtga cgatgacctg ctcaacgaag ccgaggtcga gcatgccagc gccaaggatc
     7141 tgattgcgca gatttcgggc atggatgccg acgaagagct gttcgacgcc aaggtcatcg
     7201 tgctgggtga atacatcgac caccacgtgg aagaagagcg ggaagagatg ttccccaaag
     7261 cccgcaagag cgacatggac ctcaaggcca tggtcgagga catcaaggca ctcaaggaag
     7321 agctgctggc cagctacgaa acgcccgcct gatgggaacg tcagcccgtc cccgccctgc
     7381 ggggccgggc tgaaagcccg ctaccactca ggttgcgatg gcgccgaccc ggggatcgaa
     7441 ggagaaatag aaacacgcgc ccttgccctc ggacgactcg ccgcgtatca ggccgccgtg
     7501 ccgctccagg atgcggtgca cggtggccag tccgatgccg ctgccttcga attcggtgtc
     7561 atggtgcagc cggtggaaag gtttgaacag gctgtcggca taagccatgt tgaagccggc
     7621 gccgttgtcg cgcacgaaca gcatcttgcg cccgccctcg cgcgccggca tctggccgaa
     7681 ttcgatcatc gcgtcgggct gctggcgcga gtatttccag gcgttgccga tcaggttttc
     7741 caggatgatg cgtgcgagct tgctgtcgca gcggccgcgg atgcccggct ggatgatgac
     7801 ctgcaccggg cgtttggggt cgcgttgcct ttcctgcatg accacctgct gcgccatgac
     7861 gctgaggtcg acatcttcca gcaccagttt tcccttgctg acacgggcca gcgccagcag
     7921 gtcgttgatc agctcatgca tacgcacgac gttgacccga acccggtcga acaggcgttg
     7981 ctcctcgtcg ctgagccggc ccttcaggcg ccgcgccagc agcacgctga acccctcgat
     8041 gctgcgcagc ggggactgca ggtcgtgcga gaccgagtaa ccgaaagact ccagttcggc
     8101 attgctcgcc atgagctgct cggtgcgctc gttgacgcgt tgctccaggg tttcgttaaa
     8161 gcgcgaaatt tcacggtcgc gccgcagccg caccagctcg gccgtgatgc ggctggcaaa
     8221 aatctgcatc aacgcatccc ggtcggcgga gcgggcctgg ggctttcgcc acaaggcgtt
     8281 gacgacaccg atgggcgtgc cgtcggcatc gcgcagcgcc gcaccaacgt aggcgtggaa
     8341 gttgccctcc accagaatcc ggtcgtgcgg gaaaagctgt tgcacgccct cgttgtagac
     8401 acacaggtcg gactggctga ggaccttgtc gcagggggtg ccggccagag ggtaccggat
     8461 attgggctgc aactcgccgt cctgaaccat ggccagggag gcgacgctct ggtcggccat
     8521 ggtttcaccc acgatcacca gatccgcgcc aatggccagg gccagatgct ggaccacgga
     8581 acggaagaag gcttcgccgg tgccgctcga cagggccttg gcgacatcga tcagctgggc
     8641 ttcgcggcgc ttttgttcgc tgatgttgat ggtcgagttg agcatgcagg gctcgccggc
     8701 gatatcgatg atcgccgacc agacccggca gtcgaccatg ctgccgtttt tgtggcgcat
     8761 ccgcagttcg cggctcagca cccgacctcc ggcctgcagg tcctggacaa accgcctgcg
     8821 ttctgcttcg ctggcccagg ccccgatctc gaccgctgtg cgccccacca gttcttccgg
     8881 ctcaaagccg tggatgcggt cttccgaggc gttggcggcc aggaaggtgc cgtcggcaac
     8941 ccgcgtgatg gtcatgttga tggggctgaa atcaaaagcc ttggagaaca gttcggccga
     9001 ctggcgtgcc gcctgccggg catgcgtttc cgcggttgcg tcggtgacgg tggacaccac
     9061 aatgcgtgcc gggcccgagc cctccacctt gcacgacagc aggatgtgga tcagcttgcc
     9121 agtcttggtg cgcgcatcga gcggcatgtt gatcacccgc ccggccacct ccattttctg
     9181 ctggaaaagg cggcgggccg caggatcgtc ccacaggggg gcgtcggttc cacccacaaa
     9241 ttcgctgcgc gagtagccat acagccgctc gaaggcaggg ttgacgtcga gtaccgtcct
     9301 gtcttcaagc gcctgcacga tgatggccgc cgggctgctg cggaagatgc gggtgtaccg
     9361 gtcttcgctc aggcgcagct cgtcttcggc cttctcgcgc cggcgcagtt cggcatgctg
     9421 ctcgcgcagc gcccggagca ccacctggcg gctggtgtac acgctgccgg caatgccggt
     9481 gagcagcagg acagtgacca gccagaagcg caggttcacc gtgaaatcgg ccttgcccag
     9541 caggccggcc tgatcagccc agctcagcag gcccaggacc agggcactca acacgaccgc
     9601 gaagatcagg gactttttcc cctgcatcat tcccgcggcg accaccgcgc cgacaaaacc
     9661 cagcagcagc ccactgcgga ccgacccgta ggccaccatg cccgccgccg acaggccgat
     9721 cagggaataa accagcaggt agccggtcca ctggtagtgc ccacggtgca gcagccagta
     9781 gccgagcgcg gtcagtgtca gcgcgacggc aatcaccatg atttgcacgt cgccgatttc
     9841 ctgggcaagg gacaggccca gcaccaaggg caggctggcc agcagcatca gcaacaggga
     9901 gcgcagcccg cgcgagtacc gttgttctat gagcgctgcg gccagctggc gagtggattc
     9961 gttaggagag gtggtgtcga gcatgacggg gcgcaggttt gatgaatact aaagcactgc
    10021 accacacgcc agcgattatt tgttggcggc ctcagccccc ctggcgacgg gatggccggg
    10081 gtcgctgcac cagtcgctcc agctgccggc gtagaggccg gtgcggccca ggccggcaat
    10141 ttccatggcc aggaggttgg gcacggcact gacgccgctg ccgcagtggt gcaccacacc
    10201 ggccgggtcg cggcccgcca gcagggcttc aaactccgcc cgcagctggt cggcaggctt
    10261 gaacctgccg tcgggaccga tgttgtcgcc gaagggccgg ttcagcgcgc cgggaatgtg
    10321 gccggccacc gggtccagcg gctcgacctc gccgcgaaaa cgtgccgggg cgcgggcgtc
    10381 caccagggtc tggccgggtt ggccgagccg gttggccacg gttttgccgt cgaccagcag
    10441 cgccttttcc gggccgggga aaaagatgga ctggaagtgc gaaggctctt cgccgcggtt
    10501 gaccgcgccg cctgcggcct gccaggcctg cagtccgccg tcgagcacgg ccaccgcctc
    10561 gtggccggcc cacttgagca tccaccacag gcggccgcag tagttggcgc cgttgcggtc
    10621 ataaaccacc gcctgcatgt cattgctgaa gcccacgctg gacagccagg tggcaaattt
    10681 ttcacggttc ggcagcgggt ggcgcccgcc ggaagcgggt tggtccgcgc cgctgtgcac
    10741 ggtcagcacg ccgtgggcac caggaacgcc gtgtttggcg ctcaggtcgg tgtcgaggtg
    10801 ggcatacacc gcacccggga tgtgggcatc caggtacagc tgtctgccag cctgtggctg
    10861 catcagctca aagctgcaat caaatacgcg atagggttgg ccgctgcgcg ccagggcctg
    10921 cagctgttcg gcagaaatca gggtggtgta catggcggct ccggttgaaa aataagcccg
    10981 tgaaatcgat tcagtgttct tcggcaggcg cgcccggtgc ggcccgggcg cgcagcacgg
    11041 tggccgcgat gccgctggcc acgatcagcg ccatgccggc ccaacccagc agcggaatct
    11101 ggtcgccaaa cagcaccagg ctgtagatcg cgccaaacac aatgccggag tattgcaagt
    11161 ttgccaccac cagcgtggca ctgcgggttt tcgccatgct gtaggcccgc gtcatgcaca
    11221 gctggcccag cgaggccagc acgcccaggg gcagcagcca caacgccggt ttccagtgcc
    11281 actcggagac gccggccacc agcatgccca ggccgccggc gacagccgag ccgaccgcaa
    11341 aataaaacac cgtgcgggtt tcgggttcgc cgactcgtgc cagcgccatc acctgcatgt
    11401 aggcaaaggc ggccagcagg ccggacatca ggccgatcag gccggcgaag gcctggtttt
    11461 gctctatcgt cggtcgcagc atcatcacca ccccaatgaa gccggtgatg acggtcagca
    11521 ccagcggtcc ctgagacgag atgtttcctg ccggggcttg gccgcgcgca ggcagcagcc
    11581 ccatcagcag cgcgccgccg accagaaagg tggcgatcca gacgctgctc atgtagttca
    11641 gcgtcatcgc ggtggctagg ggcaggtggg cgatggcgta aaaccaggca cccagcgaga
    11701 tcacgccgac caggctgcgc cacatgtgca tgccgggata ccgggtggcc agcgccacgc
    11761 cacgtgtgcg ggccagcgcc cacatgaaaa ccatgcccag caggccgcgg taaaacacca
    11821 gctccgcgct gttgaaatgc tccgacgcga acttgacgca gaccgccatg gtggcaaaga
    11881 agaaggcggc ggccaacatc cagagggctt gcatggcgga gctcgttgtt agaagtaaaa
    11941 agtgattgca gcccaggcgg ttcctgcggt ggcagctatc gaatgaatag cagtggctgc
    12001 atggaggggc cgcgtcaaag catttgcgtg gtcttgctgg cgcccatctc gcggcggtac
    12061 cactcgtgaa agtgctgcat gccgtcttcc atcgggctct ggtaggggcc gaactcgttg
    12121 tcgccgcgtt gcatcagggc cttgcggccg gcgtccatgc tcagggcaat ctcgtcgtct
    12181 tcgacacagg tctccatgta ggcggcctgc tgcgcatcca tgaactcgcg ctcgaaggcg
    12241 cagatctcct cggggtagaa aaactccacc acgttcagcg tcttgtccgg tccctgcggg
    12301 tgcagggtgc tgaccaccag cgcgtgcgga taccactcga ccatcacgtt ggggtaatag
    12361 gtcagccaga tcgcgccgta cttgggcggc acgcccttgc ggtactgcag caccacgtcg
    12421 tgccatttct tgtagacgtc ggtgcccggt ttgcccagct tgtcggagac accgacggtt
    12481 tgcaccgagt agttcggtgc cagctcccag cgcaggtcgt cggtggtcac gaaggcgccc
    12541 aggccggggt ggtaggggcc gacgtggtag tcctccagat agacctcgat gaaggtcttc
    12601 cagttgtaat tgcactcgtg cagtgtgatc cggtcgagct ggtaacccga aaaatcgagg
    12661 tcgggcatgg cgcccagcgg cgccatgtca gaggcgatgt cgcggccgtt gtcttcaaac
    12721 accaggccgt tccagctgct ggtctggtaa cggttcaggt tcaggcaggg gtcaacctcg
    12781 aaatgcggcg cgcctatcag ctcgccggtg gtgttgtagg tccagcggtg cagcgggcag
    12841 acgatgttgc tgccggtgtt gccgcggccc tgcagcatgg tggactggcg gtgccggcag
    12901 acattggaga tcagctcgac cccggtggca ttgcgcacca gagcccggcc gccgagctcc
    12961 tggggcaggg cgtagtagtc gcccagattc ggcacggaga gctcgtgccc cacgtaacgc
    13021 gggccgcggg caaacagctt ttgctgctcc cgctgataca ggccggcgtc aaagtagctg
    13081 gaaacgggga gctggctggt ggcctgcagg agacggaggc ttagatcaga catgcaattt
    13141 ccgaaacagg cagtttcggg ggtggatgaa ggggcagaca gccctgtagg gcaggcgggg
    13201 cgtcagggcg tgaagcctgt tgattgtacc gtgcaggtcc cgctttgtgc cgcagcgcac
    13261 ctcccgtgcg ggttcgcgtc accccataaa caggtggctt gccgcgggga caatgccgtc
    13321 cggcgggaaa acggcgcaca acggcctaaa atcagtcctc aaccaaatac ccatggccaa
    13381 aacttcctct tcccccgtcc ccgccgcccc cgccagttac gaagctgcgc tggaagaact
    13441 cgaaaccctg gtggcccgcc tcgaatccgg ccagttgccg ctggaccagt tgctcaccgg
    13501 ctaccagcgc ggcgccgaac tgctcaagtt ctgccgcagc aagctcgagg ccgtggaaaa
    13561 ccagatcaag gtgctggaag gcaatgagat caagccctgg aaccaagagt aagcccattg
    13621 actgaacaaa cacattttgt gctgggcgcc tggagcaccc gccaactcgc cgccgtcgag
    13681 caggcattga gccgctgggt ggcccccccc gaaccccggc ccgcaccggc cgggctcggc
    13741 gatgccatgc gttacgcggt gctcgatggt ggcaaacggc tgcgtccgct actggtcatg
    13801 gcggcctgcg aggcggtgga cggcagtccc gctgcggcgc tgcgcgtggc ctgtgcggtg
    13861 gaactgattc acgcctattc gctggtgcac gatgacatgc cctgcatgga caacgatatc
    13921 ctgcggcgcg gcaaaccgac ggtgcatgtg aaattcggcc aggcgcaggc cttgctggca
    13981 ggcgatgcgc tgcaggcgct ggccttcgag ttgctgacac cggacgatgg ctccatcgag
    14041 cccgcgacgc aggcgaccct gtgccgcatg ctggcccaag ctgcgggtta ccagggtatg
    14101 gccggcgggc aggccatcga cctggccagt gtcggcaagg cgctgacctc ggctgagctg
    14161 catgacatgc accggctcaa gaccggcgcc ctgctgcagg ccagcgtgct gatgggcgcc
    14221 gtctgcggcg ctgccccggc catggcacaa agcgcgctgt gcgactacgg tgccgccgtg
    14281 gggctggcct tccaggtggt cgatgacatc ctggacgtca cggccgattc ggccacgctg
    14341 ggcaagaccg cgggcaagga cgcggcccag gacaagccca cctttgtgtc gctgatgggc
    14401 ctgccggcct cgcgcaatta cgcgcaggag ctgctgtccc aggcgcatca cagcctgaag
    14461 gccagcgagc tcgggaacac cgccgcgctc agcgcgctgg ccgacatgct ggtcaaccgg
    14521 acaacttgag cacagactcg agaaaaaaag cgaccggcac ttccaggaaa cgagacatca
    14581 gaaacaagac accacgatgt acccactgct agaaaccatc aacagccccg ccgacctgcg
    14641 cctgctgccg cgtccccagc tcaggacgct ggccgacgag ttgcgcgcct atgtgctgca
    14701 cagcgtgtcg cagaccggcg gccacctgag ctccaacctg ggcacggtcg aactcacggt
    14761 ggcgctgcac tatgtgttca acacgcccga ggaccggctg gtctgggacg tgggccacca
    14821 gacctacccg cacaagatat tgaccggccg gcgcgaacgc atggccagcc tgcgccagtt
    14881 cggcggcctc agcggctttc cgcggcgcga tgaaagcgaa tacgacacct ttggcaccgc
    14941 gcattcatcg acctcgattt ctgccgccct gggcatggcg atggcggcca aacaaaaggg
    15001 ggaagagcgt catgcggtgg ccatcatcgg cgacggcgcc atgagcgcgg gcatggcctt
    15061 cgaggcgctc aacaatgccg gtgtctgcga ctgcaatctg ctggtgatcc tcaacgacaa
    15121 cgacatgagc atcagcccgc cggtcggcgc cctcaaccgc tacctggcgc agctcatgag
    15181 cgggcagttc tatgcctcgg ccaaaagcgt cggcaagcag gtgctcaagg tcgcgccgcc
    15241 gctgttcgag ctggccaagc ggctcgagac ccatgccaag ggcatggtgg tgccggccac
    15301 gctgttcgag aatttcggct tcaactacat cggccccatc gacggccacg acctcgagtc
    15361 gctgatcccg accctggaaa acatcaaaca tctgcagggc ccgcagttcc tgcatgtggt
    15421 gaccaaaaaa ggccagggct acaagctggc cgaggccgac ccggtggcct accacggccc
    15481 cggcaagttc gatccggcca tggggctgca gaaatccacc gcaccggcga aacagacctt
    15541 cacccaggtg ttcggccact ggctgtgcga catggctgaa aaggacagcc ggctcgtcgg
    15601 catcacgccg gccatgcgcg aaggctcggg catggtcgag ttccatcagc gcttccccga
    15661 ccgttattac gacgtcggca tcgccgagca acacgcggtc acgtttgccg cgggcatggc
    15721 ctgcgaaggc ctcaagccgg tggtggcgat ttattcgacc tttttgcagc gcggttatga
    15781 ccagctgatc cacgatgtgg cgctgcaaaa tctgcccgtg gtgtttgcac tcgaccgcgc
    15841 cgggctggtg ggggccgacg gcgccaccca tgcgggcgcc tatgacatcg cctacctgcg
    15901 ctgcatcccc aacatgagtg tggcctgtcc ggccgacgag aacgagtgcc gcatgctgct
    15961 gagcagcgcg tatgaacaga accatccggt ggccgtgcgt tacccgcgcg gcgccggtgc
    16021 cggtgtggca acgcagtccc acctggaggc cttgcccttt ggcaagggtg agctgcgccg
    16081 ccagggctcg ggcgtggcga ttctggcctt tggcaccttg ctgcatccgg ctctcgaagc
    16141 tgccgaaaaa ctcggcgcga cggtggtcaa catgcgctgg gccaagccgc tcgacaccga
    16201 attgctgttg cgggtggcgg ccagccatga ggccctggtc acggtggaag agggctcaat
    16261 catgggtggc gccggcagtg ccgtggcgga agcgctggct gctgcgggcg tcgtcaagcc
    16321 cttgctgcac ctgggcctgc gcgacgagtt caccgagcat ggcgaccccg ccaggctgct
    16381 ggccctgcag gggctggacg cggcagggat cgaacagtcc atcgtcacgc gttttggcgt
    16441 cctgcctgcg ccggcccaac cggccaagcg cacgctcaaa tccgtggcat gacagcgtcc
    16501 acggaaagcg gggccgggcc atcggtcatg cccgatgtgc agggccgggc cgacctgcgc
    16561 cgcctgccga tccagcgcgt cggcatcacc tcgctgcgtt accccctgag tgtggtggtg
    16621 gcgggcaagg tgcagccgac ggtgggcacc tggacactcg atgtgggcct gcgcgccgac
    16681 cagaagggcg cccacatgtc gcgcctggtt gcctggctgg acgcgttgac gctgcgcggc
    16741 gaggccctga cggcagactt gctgtgctcc gaaatcgaga gcatgctgcg cttgctggaa
    16801 gccgacagcg ggcacattga agtgacgttt ccgtttttca tccggaagtc ggcgccgatc
    16861 agcggtcagc aaagcctgat ggagtacgac ggcgcctggc tggcccggcg ccaggacggc
    16921 gcgacccgca tcagcatgca ggccacggtg ccggtcaagt cgttgtgtcc ctgctccaag
    16981 gaggtcagcg atgacggcgc gcacaaccag cgttcgcaca tcaagctgct cgtccggctc
    17041 accggggcca tcgactggag cgaactggtg cgttttgccg aggacaacgc gtcctgcgag
    17101 ctctggggtc tgctcaaacg acccgacgaa aaatgggtga ccgagcgggc ctatgccaac
    17161 cccaagtttg tcgaggacct gattcgcgac gtggccttgg cgctcgatgc cgacgatcgc
    17221 atcgccggct accgggtagc ggtcaccaac ttcgaatcga tccacgccca cgacgcctat
    17281 gcggtgatcg agcgcggcat cgtttgaacg cctgcgcaag cgcttgcacc gccgttttca
    17341 ttcctgattt tcattcctga cagtagcctg aagacatgcg atgtgagagc tgttttgacg
    17401 ggataacccc caatggtcaa gccttcccgc tttctacaat aagcatgcta acagtcaagg
    17461 ccgcatggag cggcgctgta caagaaccca ccgttttatc aactcccgga gtcttttcaa
    17521 tggatcgtcg ttccctcatc aaacatgccg gtatcgccgg tgtgcttgcc gctggtgttg
    17581 caccggccgt acacgcccag gctgccatcc gctggcgcct cgcatcgagc ttccccaagt
    17641 cgctggacac ggtgttcggc ggtgcagaga ctttcgccag aaaggtcaac gaaatgagcg
    17701 gcgggaaatt tgtcatttcc acccacgcgg ccggcgagtt gatgccggct ttcggcgtgg
    17761 tcgatggtgt gcagaacggc acggtcgaga tggcccacac cgccgcctac tatttcttcg
    17821 gcaaggacga cgctttcacg ctgagctgct cgattccctt tggtctgaac agccgtcaga
    17881 tgactgcctg gatgttcgag ggcaatggcc tgaagctgac gcgcgagttc tacgccaagt
    17941 acaacatcat gaacttcccg ggcggcaaca ccggcgcgca aatgggcggc tggtatcgca
    18001 aggaaatcaa gtcggtcgcc gacatgaagg gcctgaaatt ccgcgtcggc ggttttgccg
    18061 gcaaggtact cgagcgtatg ggcgtggtgc
//
LOCUS       DAIDFG010000025        17761 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_25_length_17761_cov_17.539704, whole genome shotgun sequence.
ACCESSION   DAIDFG010000025 DAIDFG010000000
VERSION     DAIDFG010000025.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 17761)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17761)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17761
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25_length_17761_cov_17.539704"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3269 a         5760 c         5695 g         3037 t
ORIGIN      
        1 cattgtctgg ggcggagccg tgcggctcag ccccgcggac gacatcctga ttcgcgtcga
       61 gcgcccgctg gacctggaca gccaggggca actcgtcgcc tcggtcctgt ccaagaaagc
      121 cgcggccggc gccacccatg tgctgatcga catgccggtc gggctgacgg ccaaggttcg
      181 cagcgcgcac gcggcccacc tgctggggca gcagctcgag caggtcggtc aggcactggg
      241 catgcaggtg cgtgtggtgc agaccgacgg cgtggccccg gtgggccgcg gcatcggccc
      301 cgcgctggag gcgcgcgacg tgctggccgt gttgcgccgc gaaacgcggg ctccggccga
      361 cctggcgctg cgtgcgctgc agctggccgg gcagattctc gagttcggtg gcgcggcacc
      421 cgccggggcc ggcctggccc aggccaccgc agtactggaa gacggccgcg cctggcaaaa
      481 attccaggag atctgcgagg cgcagggcgg cctgcgcgag ccaccggtgg ccacccacca
      541 gcaagccgtg cgggcccggc atggcggcac cgtgatcgcc atcgacaacc ggcgcctggc
      601 gcgcatcgcc aaactggccg gcgccccttc ggcggcgtgt gcgggcatcg acatgaatgt
      661 gcagctcggc acggtggtcg agcgcggcca gccgctgttc acgctgcatg cggcaacgcc
      721 gggcgaactc gcctatgccc tggagtacgc cacctcccag gccgaagcgg tgcatgtgat
      781 ggaagaggcc tgatgctggt cctggccatc cccggtggtg agcagctcgc ggccggcctc
      841 gccagccgcc tgcgctgcga ctacgccagc ctgcaactgc accggtttcc cgacggcgaa
      901 accggggtgc gtatcgatgc gccggtcaaa ggccgctgcg tggtgctggc cggcagcctg
      961 gaccagcccg atggcaagac cctgccgctg atttttgccg ccgacgcggc gcgtgagctg
     1021 ggcgcccggc aggtcggact ggtcgcgccc tacctggcct acatgcgaca ggacaaacgt
     1081 ttcaagcctg gcgaagcggt gacctcccac agctatgccc ggctgctgtc cgcttcgctg
     1141 gactttctgg tcaccgtgga cccgcacctg caccgcatcc acagcctggg cgaggtgtac
     1201 agcatccgca cgcaggtcat cccggccgca ccgttgattg cgcgctggct gcgcagccac
     1261 gtggaagcgc cctggctgat cggacccgac caggaaagcg aacaatgggt cgccgaagtg
     1321 gcaggcttgg ccggcgcccc ctggaccgtg ctggccaaga cacggcgggg cgaccgcgat
     1381 gtgggtgtcc ggctggccgg ccgcgaacgc tggccgggac ggaccccggt gctgctcgac
     1441 gacatcattt ctaccgggca aaccatgctg gccgccacgc ggctccttaa aaaagccggc
     1501 ttgggtgcgc cgctgtgcat aggtgtgcat gcgctcttcg acgccgaggc tcaacgccag
     1561 ctgctcgaag caggcgtcac gcgtgtgctg acctgcgaca ccatcacgca cccttccaac
     1621 gcgatacgca tggccccgct gctggcccgc gccgtgcgcg cgctctgcgc cgacaaaaag
     1681 gatgccaaac aatgacagcg aaacgcatcg ctctgatcca gggccatccc gattccacca
     1741 tgccccacct gtgccacgcg ctggcggccg cctatgccga gggcgcgaaa gaagcgggac
     1801 ataccgtgcg tgtcatccag gttgccgaac tcgatttccc gctgctgcgc agccagcagg
     1861 cctgggagga agacccgctg cccgccggct tgaagcaggc gcaggtggac atccgatggg
     1921 ccgaacacat cgtgctgttc tttcctctct ggctgggcgg catgccggcc ctgctcaagg
     1981 gttttctgga gcaggtcgcc aggcccggtt ttgccttcga gcacgatacg ggccacgcga
     2041 ccttcaccaa aaaagggctg acaggccgtt cggcccgtgt tgtcgtgacc atgggcatgc
     2101 cggccctggt gtaccgctgg tatttccgcg cgcacagcgt caagtcactg gagcgcaaca
     2161 tcctcggctt cgtcggcatt gcccccgtgc atgaaacgct gatcggcatg gccggcaact
     2221 tcaaggcgaa cgacgccggc cgctggctgg ccaaactgcg gcggctcgga caacgcgctc
     2281 aataagatca acaagggatg aatggccaac cccactccat ccactcagga aagaccccca
     2341 tgaaccctgc acccatggcg ccaccccttg cgccccccat cactcccgcg cccggcaagc
     2401 ccggcagcga catcgtcaag cgggtcggcc tggtcgccgc cctggcggcc ctgctggcca
     2461 tcgtctggat gccggcactg cagggcctgc ccgcggccgg gcaggtgatg ctggccatcc
     2521 tggcctttgc cgtgattgtc tggatgaccg aggcgctcga ctacgccgtg agctcggtgg
     2581 tcataggcgc gctcatgatt ttcctgctgg cctatgcacc cgatgcggcc aggcccgccg
     2641 gccccgacat gggcaccggc gccgcgctcg gcctggccct gtccgggttt tccaacagcg
     2701 ccgtggcgct ggtagctgcg gcctgtttca ttgccgcggc gatgtctgcc accgggctgg
     2761 accggcgcat cgcgctcaag gttctgtcga aagtggacgc gggtaccaac cacatcgtga
     2821 tcggcgccat ggtgacgggt ttcctgctct ccttcatcgt gcccagcacc acggcgcgtg
     2881 tcgcctgcct ggtgcccatc atgatgggct tcatcctggc tttcaaggtc gacaggcgca
     2941 gccgttttgc cggcctgctg gtgatcaccg cggcgcaaac cgccagcgtc tggaacatcg
     3001 gcatcaagac ggcggcggca caaaacatgg tggccatcgg tttcatcgag aagcagttcg
     3061 gcaccagtat cagctggacc gagtggttta ttgccggtgc gcccttttcg gccgtgctga
     3121 gcgtggcggt gtatttcatc atgacccgcc tgatgaaacc ggagatgaag gaaattgcag
     3181 gtggccaggc caccatccgg caacaactcg aagcgatcgg gccgatgacg ctgcggcaat
     3241 ggaagctgct ggtcatcgtg ctggtgctgc tgggcttctg ggccaccgaa aaagtactgc
     3301 acaacttcga cacctcctcg accaccatcg cggccattgc cctgatgctg gtgccgcgcc
     3361 tgggcgtgat ggactggaag gagtcgcagc gcggcttccc ctggggcacg gtgatcctgt
     3421 ttgccgtcgg catcagcgtg ggcaccgcgc tgctgaaaac aagcgcggcc ggctggctgg
     3481 ccaacctgat cgtccagcac ctggggctgc agcaggccag cgcgtttgcg atcctgatgc
     3541 tgctgtcgct gttcctgatc gtgatccacc tcggctttgc cagcgccacg gcgctggcct
     3601 cgaccatgat ccccatcatc atcagcgtgc tgacggcggt ccagacgccg ggcatcaacg
     3661 tgatcggcat gaccatgctg ctgcagttcg tggtgagttt cggcttcatc ctgccagtca
     3721 acgcgccgca gaacatgatc gcctacggca cggacacctt cgacgcacgc gacttcgtgc
     3781 gcaccggcct ggtgatcacg ctggccgcca tggcgctgct ggtcatcttc gcgctgacct
     3841 actggccgtg gatgggctac atgaccaaag cggtttaggc agaaacagac acaaggagca
     3901 acgacatgac caccctgacc aagctcgccc tcggcatgat gggcaaactg cgaccgcagc
     3961 cggccttggg caacgcggcg gcccagatca gcctgccgcc gcccgaccgg caggccgggc
     4021 tgccgttgat gcaggccctg gcccagcgcc agtcgcgccg cgactacaag cctgacccgc
     4081 tgccgctcga gttgctgtcg ggcctgctct gggccgcctg cggcgtcaac cggaccgatg
     4141 gcggacgcac cgcgccgtcg gccctcaacg cgcaggaaat cgacatttat gtggccttgc
     4201 ctgaaggcac ctatttctac gacgccgccg ggcatcaact gcagctggtc gccggcgccg
     4261 acctgcggcg tgtgaccggc taccaggatt tcgtggatga ggcgcccatg gacctggtct
     4321 acgtcgccaa ccacccgcgc atgtcgctgg tgccggccgg gcagcgcgag gcctacgccc
     4381 atgtcgccgc cggcgccatc acgcagaacg tctacctgtt tgcggccagc agcggcctgg
     4441 ccacggtgat acgcgcctgg atagaccgcg ccgccatcgc cgacgcgctg ggcctgagcc
     4501 atgaccagca ggtcctgctg tcgcagacgg tcggttatcc ggctgctgct ccctgaaagc
     4561 gcggcgagga gctccgcgaa cgctcctgtt tttatagcgt cagcgtaata tccacggtcg
     4621 ccagggccga ttttgactga atcaggccga accgctactg tcttcaccga tcagcagctt
     4681 gccgtcagcg atgtatcggt gcggcggcac ttccaggttg gtgggcgccg gcttgttcct
     4741 gaacacccgc ccggccaggt caaaggttga gccatggcag gggcagaaaa atccgcctgc
     4801 ccagtccgca cccaggctgg ggttgccggc gccggcgggc acctggttgg gcgaacaacc
     4861 caggtgggtg catatgccca cacccacaaa cacctcgggt cggatggagc gctgcgggtt
     4921 gcgcgcgtag tcgggctgct gggcttttct ggagccggga tcggccaggc tggcatcgag
     4981 caagggcagg ctcgccagca tctcgggcgt gcggcgcatg atccacaccg gcttgccgcg
     5041 ccactccacg gtcttgatgc cgccgggcgg tatgtcggaa atatcgactt ccacgctggc
     5101 acccatggct cgcgcacgct cactgggcgc gaaactcgag acaaaaggga cagcgacggc
     5161 cgccatgccc acacccgcca ccgctgcggc aagcagcacc acctggcggc gctccgcatc
     5221 gtgggcaggc atgtcgccgg caggggccgg gctggctggt agttcaggaa aggtgaaagc
     5281 cggtgtcatg atgttcctct gccgtgtcac ggtgaaggca ctgcgctttc gccgtgtttg
     5341 gccatcaccc tgttctcaag aaacgtgccg ccccggctct ggggaaaaaa gccaattcaa
     5401 tcaagggctg ctgcttgttc accgaatgtc cggctccgtc acgactgtca tttttggcag
     5461 tttctggcag tttctggcag ccggcagtac actgctggca gcatgaaaaa cctcgccgcg
     5521 caacccctgc cggaactggc gtcctacctc gaaggcctgg ccgaacctca catcctgctc
     5581 gacgcgcagt accgcatcct tgcggccaat gccgcctacc gccgccagtt cagcgccggg
     5641 cgcgaggtcg cgggccagct ctgccatgag gtctcgcacc acttcagcgt tccttgcgac
     5701 caggccggcg agtcctgtcc gctggcccag tcgcgcgaat ccgggcagcg tgagcgggtg
     5761 ctgcacctgc atcacacgcc acggggcgag gagtacgtca acatcgagct cgcgccgctg
     5821 cgagaccaca agggccggca aacttttttt gtcgagaaaa tcgcgacgca gcgcgcggcg
     5881 cagggccagc ctgcggccca gggcctgatc gggcgcgcgc cggccttcca gcacatgctg
     5941 gcgctggtcg cgcgggttgc accgtcgcag gccacagtgc tgctgctcgg ggaatcaggc
     6001 acgggcaagg aactggtggc ccgggcggtg catgagacca gtccgcgcgc ccagcgggcc
     6061 ctggtggcgg tggactgtgc cagcctgccc gacaacctgt ttgagtccga gttgttcggc
     6121 cacgaacggg gtgccttcac cggcgcaaac acagcccgag ccggcctggt ggaagccgcc
     6181 agcggcggca cgctgtttct cgatgaggtc ggcgacatac cgctgaccat gcaggtcaag
     6241 ctgctgcgcc tgctggagac cggcacctac cgccgcgtcg gcagcaccga gctgcgccat
     6301 gccgacatcc gcgtggtcgc cgccacccac cgcgacctgg agcgcatggt ggccgaggga
     6361 cgtttccgtg aagacctcta ctaccggctg agcattttcc ccatccacct gcccgccctg
     6421 cgcgagcggc gcgaggacat tggcctgttg gcagcggccc tgctggcgcg cgtggcgcca
     6481 cagcgcaagc tgcagatcag cgaacctgcc ctgcatttgc tgcaagtgca ggacttcccc
     6541 ggcaatgtac gtgagttgag aaaccttctg gagcgggcgg ccttgctatg tgatggcgat
     6601 acgctggagg tcgctcatgt tgagcaggcg ctgatgtcgg gccggcgccc ggtcaacgca
     6661 ctgcagcgcc agccgcaagt ccccgccgcc gacactgacg cccgcggcct ggcgcacgcc
     6721 accttggcaa ctggctcgct caagaccatc gagcgcaagg ctctgcatca gttggtggcc
     6781 agccatcgcg gcagtcgcgc ggagctggca cagaagctgg gcatcagcga gcgctcgctc
     6841 taccgcaagc tcaaggcact tgagtagcgt tggacaaagc tgtgggttcc ccagagattt
     6901 gggcccggat tcagcaataa ttaagaaatt gcttatgaat tgagattcca tgaaacaagc
     6961 catattccag tttggacagg accacccgga ctacctggtg cccgttctca acgagcgggc
     7021 cgtgcgtgcc agcgccggca tttttttctt ctttgccatg attgcattca tgaacgcctg
     7081 gctactgggg aactttctgc ccacgcgggt ttttgtagtg gtttttttga tggacttcag
     7141 catacggatc ttcatcaatc caatccacgc gcccagcctc atcctggggc aatgggccgt
     7201 gcgcaagcaa caaccagaat gggtgggcgc gccgcaaaag cgctttgcgt gggccatcgg
     7261 ttttgtgctg gcgctggtca tgttgtacct gatggtgctt aacagcgtgg tggggcccat
     7321 caacctgctg gtctgcggtg cttgtctcac tctgatgttt tttgaatcgg cgttcggtat
     7381 ctgcatcggg tgcaagcttt acaacgtgtt caacaaagac aaggcccagc tctgccccgg
     7441 tggtgtgtgc gagattcccg ctggcactgg ctcaaaagtc agcttggccc aagtggcggt
     7501 ggtggtggtg tttgccgggc tgaccgcaac cgtggcacag gcggtgtacc gcaccggcgg
     7561 gccgccatcg caacacgccg cgcctgctaa cccggcctcg cccaccgaat cagacaaccc
     7621 ggccgatgcg cagcgctgca aggtgccgga gttcgccaaa gccatcgggc acgaggaaaa
     7681 atggaaactc cacaacaact gccaatgaca acctgcgtgg aaacaggcat tcgtttgcta
     7741 ctcttaatat agctatataa ccaataaaca cgagcgctag atccatattt ttcacaaact
     7801 tcatgtcatt tgcctgcaaa ctccgggtcc aatcgattca tcacgacttc actgcatgaa
     7861 cacaccactg atcaccgacg aactcgactt gctgcaatcg ctggtcgacc tgaaccagca
     7921 cccgcagatc atcgaactgg gctgcggtgc cgcacacctg tcgcgccagt tgctggctcg
     7981 atggccaagg tgcgaagtca cagggctgga ggtggacgag cgccaacacg ccaagaacct
     8041 gctcaacccg cagcagcgcc tgcaattcgt tcaggcgggt gcgcaggcca ttccctttga
     8101 ggcgcaaggt ttcgatctgg cgctgatgct gaaatccttg caccatgtcc cgctggatca
     8161 gctgcgccaa gcgctggcgg aagtgcaccg tgtgctgcga ccggaaggcc tgctctatat
     8221 ttcagaaccg gtgttttccg gagcgctgaa cgaggtcatt cgcctgttcc acgatgagga
     8281 gcaggtgcgt gccgcagcgt tgcgcgcagt gcgcgaagcg gtggcttcag gcgcatggga
     8341 gcaagtgacc gaaaccttct tcgacatgcc ggtgcactac cgcgactttg ccgagttcga
     8401 acagcgcatg atcggcgtga cattcgtcga tcaccggctg gacgccccta ccttgcaggc
     8461 tgtacgtgaa cgctttgagt cgcacatgac agcggacggg gcacacttcg cgcgcccgat
     8521 gcgtgtcacc ctgctacgca agtgctgatc tcgtcccagc cgtgctaaag ccctggcgaa
     8581 tcggtgtagt tggcaaccaa tgcagcgggc ggcccgggta gggctcctga aatggcctgc
     8641 cgcaaaaacc cgatcagtgc cggcagatgc accgccagcg cggtgctggg atcatgcccg
     8701 ccggtgacag ccaggaactg cacctggccg ggcgacccgg cagcctgcag ccgctgggca
     8761 tcgccaaagg gcaccacggc gtcgtcagtg ccatgcacca gcaacaccgg gcaacgcagg
     8821 cgggccatgc tggccagtgg ggcaatgtca tcaaagcgcg cgccaatcac cgcctgcaca
     8881 tggcgcatca cgtaccagcc cagcacgggg taaggaatac gcgcctctgc cagtagcgag
     8941 cgcatgatgt cgcgggggtg ggcgaaggca ctcaggctta ccacagcctg cacatccggt
     9001 cgccgcgttg cacacagcag ggcggctccg gcgcccaccg agtggcccat gacagccagg
     9061 cgcctcttgt caatgtgcgc ttgctggtgc agccagtcca gaccggcttc aatgtcttcc
     9121 gcaaaccggg gcagcgaggt aaacgttgcg tcgtcactct ggccgtggca gcgggcgtcg
     9181 accagcagca cggcaaaacc ggcagcgtgc agtggcgcca acgctggcaa catcaggctg
     9241 gcgttggcgc cccagccgtg catcaccacc accgccgggc ttggcgtctg gccgggtacc
     9301 ggcacaaacc aggcaaacag cgttttgccc tgcgccgtgg gcaagcgcac cacctcggcg
     9361 gccagcccca catcacccgg cgtgcgttgg tgcgccagcc gtggcgcccg cagcccgcgc
     9421 aagatgagcg cgtgggcacc gcggcgcagc gccagcgcgc tgatcaatgc gatggcgctt
     9481 gtgaggagca tcaaggaaaa agggccaggg cctggcgcag ttgagcttcg gtccgcaggt
     9541 actgcacttg aatcacctgg ccgttgtcaa gcgtccatgc ggcggcggcg ccagcaatgg
     9601 cggcagtcca tggggtcaac tggatcttca tgattacgca tcaggttcgg cggcaggtgc
     9661 cagacagaga cgctatcgta ggctggctga tgcacatatc agcgcgcctg ggtgttcaaa
     9721 gcttgacctg gcccaaacag ttcatgatga gaggaacgcc gccctgcaac gaccggggag
     9781 tgctgttcca gccgacgctg gcggtccttt aacaacagcc cggctgaaac attggcgcgc
     9841 attgaaactt gccgctcgcc gtgtgggctt cctgcccctg gactatcggg gcgattgtta
     9901 aaaatgacat tcaaaacgcc tcccggtaag tacactgaat cttcgatcga aaaggtggca
     9961 cgcggtgttt gaaatctttc aaacagtgcg gtacgacagg ctcttcacag cagatcgtct
    10021 cactttggcg aacttcaatt gacgatgcca tgaacggaga tttatatgga cctgaagaat
    10081 ctgaacactg ctggcaaatg cccggtgatg cacggcggta acacggccac cggcaactcg
    10141 aacatggcgt ggtggccgaa ggcgctgaac ctggacatcc tccatcagca cgacaccaag
    10201 accaacccca tgggcgagga cttcaactac cgggaagaag tcaaaaagct cgacgtcaca
    10261 gcgctcaaga aggatctgac cgccttcatg accgacagca aggactggtg gcccgctgac
    10321 tggggccact atggcggcct gatgattcgc atggcctggc actcggccgg ctcttaccgc
    10381 atcgccgacg gccgcggcgg cgccggcaca ggcaaccagc gcttcgcgcc gctgaactcc
    10441 tggcccgaca acgtgaacct ggacaaggcg cgtcggttgc tctggcccat caagaaaaaa
    10501 tacggcaaca agctcagttg ggccgacctg atcgtcctgg ccggtactgt ggcctatgaa
    10561 tccatgggcc tgaagacctt cggatttgcc tttggccgga ccgacatctg gcatccggaa
    10621 gaggacatct actggggctc cgaaaaagaa tggctggccc ccagcggcag cgaaaacagc
    10681 cgctactccg gcgagcgcga tctggaaaac ccgctggccg ccgtgatgat gggcctgatt
    10741 tacgtcaacc cggaaggcgt cgacggcaag cctgacccgc tcagaaccgc gcaagacgtt
    10801 cgcgtgactt ttctgcgcat ggcgatgaac gacgaagaaa ccgtggccct gaccgccggg
    10861 gggcacacgg ttggcaaatc ccatggcaat ggcgatgcca aactgctggg ccccgtcccc
    10921 gagggcgccg atgtggacga acaaggcctg ggctggatga acaaaaccac ccgcagcatt
    10981 ggccgcgaca ccatgtccag cggcatcgaa ggcgcctgga cgacccatcc gacccagtgg
    11041 gataacggct acttccagct gctgctcggt tatgactggg aacttaccaa gagcccggct
    11101 ggcgcctggc agtacaagcc ggtgaacatc cggccagaag acatgccggt ggacgtggaa
    11161 gacccctcta tccgctgcat gcccatgatg accgatgccg acatggccat gaagatggac
    11221 ccggcctacc gggtcattgc cgagcgtttc cacaaggacc ccgactactt ctcgaaagtc
    11281 tttgcgcgcg cctggttcaa gctgacccac cgcgacctgg gtccgaaggc ccgctacatc
    11341 ggacccgagg tgcccgagga aaacctgatc tggcaggacc cggtacccgc aggccgcacc
    11401 gactacgacg tggcagcggt gaaggcaaaa attgcagcca gcggcctgag cgtcagcgac
    11461 atggtcacca ccgcctggga cagtgcccgc acctaccgtg gttcggacat gcgcggtggc
    11521 gccaacggtg cccgcatccg gctcgccccg caaaaagact gggaaggcaa tgaacccgcg
    11581 cgcttggcca gggtgctggg cgtgcttgaa ggcattgcca ccgggagtgg cgccagtgtg
    11641 gcggatgtga ttgtgctggc cggtaacctc ggcgtggagc aggcggccaa ggccgcgggt
    11701 ttcgacatca cggtagcgtt cgctgcgggc cgtggtgatg cgacccagga tgccacggat
    11761 gtcgaatcgt tcgaagcgct ggagcctgtc catgacggct accgcaactg gctcaagaag
    11821 gactacgcgg tcagtgccga agagttgttg ctcgaccgca cgcagctgat gggcttgacc
    11881 gcccacgaga tgacggcgct ggtgggcggc ctgcgcgttc tgggcgccaa ccacggcggc
    11941 acacggcacg gcgtgttgac cgatcgcgca ggtgctttga cgaacgattt cttcgtgaac
    12001 ctgaccgaca tggcctacac gtggaagccc accagcaaaa acctgtacga catctgcgac
    12061 cgcaagacgg gtcaggtcaa gtggacagcc acccgggtgg atctggtgtt cggctccaac
    12121 gccatcctgc gcgcctatgc cgaggtttat gcgcaggacg acaaccagga aaagttcgtg
    12181 aaggactttg tcgccgcatg gaccaaggtc atgaacgctg accgcttcga tctggcctga
    12241 gctctggtcg acatccccac gccgtgcatc aaggtggcgt gggggccatc cggccccttc
    12301 gagcgccatg gcacccggat ctgctgcggt cgcgccctgc accgtcccag cctctgccaa
    12361 ggccatggtc cgtgagggaa cgtggcagtg gcacaagcac ttcctgtgac cagaatttgc
    12421 tatctattag atagctactg gcgcaataca ggcaaggtct gaaggcactt ttcatcacaa
    12481 tcaggacacc tgccgcggca ggctcagggc tcccgtgcgc cagcgcgtgc ggcggtcacc
    12541 ctgcgccaga gtcgcagaac caacagcacc aggggcatgg cgtgaatgaa caggtcgaag
    12601 atgtccaggg gtcgggacag cgtgccctgg cccagcatcc gcaatttttc aatcaggtgc
    12661 ggttcgggcg tgatcggcgc cacggccatc cagagcgcca ggacgaccag ccagaccagt
    12721 ggaatgcggt ccagccacct catgaccaga ctccctgcgg ccgctgcggg ccacgcgggc
    12781 aaacgtgcag gacatcattc aaaggacggt gcctccggct tgaccaccgg cttgccggcc
    12841 gccacccagc catcgaagcc gccggcaatc gactgcacgt tcaggtaacc catctcgtgc
    12901 agggccgcgc cgcacagggc ggcgcgtccg ctggtcttgc agtacagcac gatcttcagg
    12961 tcgcgcggtt gcagcgccgg ggtgctgctg aacttgaact cgagcaggcc gcgcgggacg
    13021 aggaccgcac cggccaggtg gccggcggcg tattcatcgg cttcgcgcac gtccaccagc
    13081 acgtcggcat cgcgcagggc ggtttcagcg tctgacacgg taatttcctg cacgcgggtt
    13141 ttggcggcgg cgacgagatc atgggcggtt ttcatgggta gctcctggct tcaagtgaat
    13201 aaaaaaattg ggctctgttt cgcacgcacc ccttgcgggg gtgcagaaaa tttcatctca
    13261 gacgctggcc aacccagcgc gtgacatggg ccgggccctg atggcccggg ccttcggaca
    13321 gggcagtgtc gccctcccat gacagcaccg gagccagcag gccggcaaaa tcgccggcca
    13381 gcatctcggg ggtgtaaagc atgtcggggt ctttggggcc cccgctggtg tgcgccagtt
    13441 gtgcgggatg aaaggcctcc agcaccagcc agccgccggg gcgcaggccc agcgccaagc
    13501 ggcggtgggc tgcgcaacgg atggcgcccg gcaggtgggt gtaaatcagc accacggcgt
    13561 caaaggcagc cggtgggggc gaccactcac ccaggtcggc cagtatggtg gtcagcgcga
    13621 caccgtggct ggctgccagt tcgcgcgcct tcttcaggcc gacctccgaa tagtccaccg
    13681 ccgtcacgcg gtgcccctgc cgggccagcc agacgccatt gcgtccttcg ccatcacccg
    13741 gcaccagcac gtcggcgggc ggcgtcaggc ggtcggcctg ctcacgcaga aaggtattgg
    13801 ggctggtgcc gtatttgtag tccggaccgg aataacgctg atcccagaag ttcatggtgc
    13861 gcgaattttt tcgtggatgc ggcgcgtggt gcgccacacg ccccacaaga ccagcggaat
    13921 cagggcgccg gcggcgatct cagggttgat gggcgcaccg gcagccttga acgccttgcc
    13981 cacatacagc aacaggctca ccacgtaata cgagatggcg gcaatcgaca agccctccac
    14041 cgtggattgc aggcgcaatt gcagttcctg cccgcgcgtg agtttttcca gcagttgctg
    14101 gttctgcacc tcggtggcaa tgtccacgcg cgtacgcagc agcgcgctgg tgcgcgacac
    14161 ccgctcggac agggaagcca gccgctgtgc cgtggcctct accgtggcca tggccggcga
    14221 caggcggcgc tgcatgaatt cacccaggag ctgggtgccg gaaatgggtt tctcgcgcag
    14281 ctccgcgatg cgctggccga ccagcgcgct gtaggcgcgc gtggccgaga agcggtagct
    14341 gtgctcggcc gtggcgcgct ccacccgggc cgccagcgac accagggtgt ccagcagctc
    14401 ctggtcggag gccgatttgt tttcgagccg cgccgtgatg tcggccagtt gcccttcggc
    14461 ctcggccagc atggggccca gcgccttggc cacgggcagg ccgcgcagcg ccatcaggcg
    14521 gtaggtttcg agttccagca ggcgctggga gatgcggccg gcacgcatgt cggtcatgac
    14581 cggcggggcg atcaccaaca tgcgctcgaa accgtcgacg cccaggtgaa atgcggtcag
    14641 cgcccacgag tgtccgttgc ccagctgcga agccaccacg gcacggcctc cgaaccaggc
    14701 cagcgcctgt gccatcaggg ccccctcgcc ggacagatcg ccatgaacca tggccagttg
    14761 gatggcggcc accgttttcc cgggaatgcc aggcagccag cccggcgcga ccaccggggc
    14821 cgacagcagg ggcggctcgg tggcacccaa cagcgcgctg gcgggcagcg actgcacgat
    14881 ggcgtaacgc gtgaactcgg tgtgccgctc ccactggacg aggtaaccct cgcagcgcag
    14941 gctcaggaag ttgccctgca ggctgtccag cggcaaatcc tgctggcccg gcaggcggcg
    15001 caggtgcgcg cactcctgct cgcgcgtcac gccggcattg agcaccgcca cgtagatgat
    15061 gagcgccggc agccgcacgc gggccgaggg tcgcgcatgc agttcgttgt gcaacacggc
    15121 acgctgcgca tcgtcgggcg gcaacaaggc gaaagatgca gcgaaggaag ccgcagaaga
    15181 aggcaggctg gaagtcatcg cctgatgcta ctgttttgat agcagcctgc gctttcccgg
    15241 caagggatga tgcctctttt catgaagaat ccccctgtgc ctggaagcgg aaggtgtgga
    15301 agaccggcac gatttatttc aggcgctgac cggtactgtc ggaaatctcg atggtgatcg
    15361 gaatgccctg tcccacactc tgggtgacgg tgcagaacgc ctcgaactgc tcgagcacgc
    15421 ggtccaggtg ctgcagcgag gcagccggca cacccagcgt catctgcgct gtcatggcca
    15481 gcacccgcag gcgcccttcc gggttgcggc ccacctgcga ggtgatgtcg cagcgcatcg
    15541 gctccggggc ctgcttgaac ttgcgcagcg caaacagcag cgaatcgctc aggcagttgc
    15601 cgacggcggc gctgagcagc tgcaccggcg agggcccgag acccgtcccc aggggcgccg
    15661 gttcatcgcc ggtcagcacc ggaatggctc cgccaaactg gatgtcaaag cggtagtcgt
    15721 gctgctgaac caggctgacc tggacgaggg tgtcgctcat ggcgcctccg ggttgaagat
    15781 gaacaggatc aggtaccgca ggaaaacttg gactcgggca cgcccagttt cttgaggatg
    15841 aagccgagcg ggcacatgtt ggtgaacccg aactggaaca ggttgaagcc cacaaaggcg
    15901 gtaaaggcca gggcccactt gctcacaaac agcgggctgc cctcgatgcc gagtgcgagc
    15961 gaagccagga tgaagaggcc ggcaatgatg cggatgtaac gttcgacggt catgaaattc
    16021 tccagagagt taaaaaaaca aaaaaacggt ttttatttca ggcgcacgat gcccaggagc
    16081 ttgagcacgt acttctcata caccggctcc gagttgccgg ttttcatctt gcggatgaaa
    16141 tacttctcga acgcgatctt ggccaggtgc acccatttgc ccttcttgaa ccagttcacg
    16201 ttgcgcggcg gtatctgcgg cagggccaca aaggccgcgc ccgtgtcgcc catgtcggcc
    16261 aggcagatcg cgttccaggt agccttggcc tgcaccggcc ggccggcaat ttcgtcggcg
    16321 atgttgtggg tgatcgcggt caccatggtc tctatcatgt aaccggtctt gggcgcaccc
    16381 gtcggcacag gcgtgacctc taccggcggg atggcgacgc agacgccggc cgcaaagatg
    16441 ctgggccagg ccttgctgcg ctggtgttcg tcgatcagca caaagccgcg cgggttgcac
    16501 agccccggca ccgccgccac cgcgtccacg cccttgaagg ccggcagcat catggacagc
    16561 ctgaacggga cttcatgctc cttgtacaca ttgccgaggt catcgacctg ggtgacaaac
    16621 attttcccgt cctcgacacg cgtggtcttg gcgttggtga tccacttcat gtcgtggccg
    16681 cgcatctccg actccatcat ggacttgctg tcgccgacac cgcccaggcc caggtggccg
    16741 atgtagggct cgctggtcac gaaggtgatg ggcaccaggt ggcgcagctt gcgcttgcgc
    16801 aggtcggcgt cgaggatgaa ggcgaattcg taggccggcc cgaaacagct ggccatcggc
    16861 atggcgccaa tgacgaccgg gccgggattc ttgaggaagt cctggtattc cgcccagaat
    16921 gcttcggcgt gcgccacggt gcacacggaa tgggtgtgtc cgccgtgggg gccggcaccg
    16981 ggaacctcgt caaagctgag cttgggccca gtggtgatca ccaggtagtc gtagtccagg
    17041 ctgtcgccgc cttccagcgt caggcgcctg gcctcggcgt cgatggtggt gacggccttg
    17101 ggcacaaagg cgatgccctt gcgctccagc aggggggcga tcttcagcac gatgtcgtcc
    17161 cgcgtgcgcc agcccaccgc cacccagggg ttggagggca cgaactggaa atagtccacc
    17221 gcgctgacca ccgtgatctt gtgctcgggc ggaagcagct cccgcatttc gtaggcagcc
    17281 ggcatgccgc ctattccggc acccatgatg atgatgttgg ccatgtcttg tctccttggt
    17341 tcaggttgaa aaatcagtcc gtcgctcagg cttcaatgcg cgcagccggc ggggccaccc
    17401 ggtgctggta cagcgcgaaa taaagaaccg gaataaccac cagcgtcagc agtgtggaca
    17461 ccaggatgcc gaagatcagc gcaatggcca ggccgttgaa gatgggatcg tccaggatga
    17521 aaaaggcgcc gatcatggcg gccaggccgg tcagcgcaat cggctgcgcg cgcaccgcag
    17581 ccgaactcac cacagcctcc ttgaaggcca tgccctgcgc gacctgcagc tcgatgaagt
    17641 ccaccagcag gatggagttg cgcacgatga tgccggccag cgcgatcatg ccgatcatgc
    17701 tggtggccgt gaactgggca ttgagcagcg cgtgccccgg catcacgccg atgatggtca
    17761 g
//
LOCUS       DAIDFG010000026        17567 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_26_length_17567_cov_18.085827, whole genome shotgun sequence.
ACCESSION   DAIDFG010000026 DAIDFG010000000
VERSION     DAIDFG010000026.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 17567)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17567)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17567
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26_length_17567_cov_18.085827"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2950 a         5896 c         5645 g         3076 t
ORIGIN      
        1 cgccggcaag gcggacaagc tgcgtgaagc cttgcagccg caggacgctc aggaggcgtc
       61 caagcccccg gccggagcgt cacccagggt gaaatagaac gcggccccgt gccccggcgc
      121 ggactcggcc cacacccgcc cgccgtgccg cgtgatgatt ctgtgcaccg tcgccaggcc
      181 aatgccggtt ccggcaaact ccgagacgtc gtgcagccgc tcgaaagcgc cgaacagctt
      241 ctcggaatag gccatgtcaa accccacccc attgtcccgc accacataca ccgcctcacc
      301 gccagcgctt tgctctcgcc tgaacgcgat cagggattgc ggctgctggc tgctgaactt
      361 ccaggcattg cccagcaggt tgtccagcac ctgcagcagc aggcgcgcat cgccctgggc
      421 cagcagttcc ggctggatgt cgagcagggc cgcgcggccg gggtccctct cgcggtaacc
      481 ctccagtatc ttttgcgcca gggcgctcag gtcaacggtc tcccagcgca ggctggtgcg
      541 cgagacctgg gccagcgaca gcagggcgtc gatcagctca cccatctgca ccacgccggc
      601 ccggatgcgg gccaggtaat gcctgctgcg ttcgcttgct gcgccgccct ccagttcccg
      661 cacgagcagg gtgctgaatc caccaatgga gctgagcggt gtgcgcaggt cgtgcgacac
      721 cgaataggaa aaggccgcga gctgcttgtt ggcaaactcc agttgcccgg tgcgctgctg
      781 cacccggtct tccagactgg cattgagttg caggatttcc tcgcgcgccc gcttgcgctc
      841 gcggatgtcg gtgttgatgc ccagcacgcc gttgacccgg ccttgcgcat cacgcaccac
      901 ggtccagcgc gcctcgatgc tgatgggtga gccgtccctc gccacctgct cgagctcacc
      961 gctccagtcg ccgtcgttgg ccaatgtctg gttcatggcg tcgatcagca cctgggggct
     1021 gcggtacatg tattcgtcca tggttttgcc gagcacctcg tcggaggtcc agccgtagag
     1081 gcgctcggcg cccttgttcc agaaacgcag ggttctgtcc aggttgcgca ccatgatggc
     1141 gtcctgcgca cggtcgagca gcgaggcctg ctcgcgcacc tgggccgccg ccaggcgcgc
     1201 cgcctcctgg ctgcgctgac gctccagccg ggcgcgcagg ctgccgatgc caaacgccag
     1261 gttgtcagcc atgtcctgca acagcttgat ttcatcgcca gcgagttttt ccaccgaatc
     1321 cgagtacagg cccagcaatc cgaaagtatg gttggcgtcg cgcaggggca ggaacaccgc
     1381 actgcgatag ccgcacgcgg aggcaatatc gctccagaaa aactggttgg caccctgcgt
     1441 gatgtcttca ctgacgcggg gctgtccact gcgtatggtc tggccggccg gcccgcgccc
     1501 ggtctgttgc tcctcgtccc aggtcagctt gatggccgac agatagccct cttcatggcc
     1561 cgcatgggcc atgggctcga tgctgcaggc cgcatcgtcc cgcgcgtaac cgacccacgc
     1621 catgcggtag ccacccacct ccaccgccag ccggcacacc tgcagcaaca gatcggcttc
     1681 gtcgtcgatg cgggtcagcg cctggttgca gcggctgagc atctgcagcg cccggttggt
     1741 ccgcgccagt tcgcggtccg cccgtttgcg gccggtcacg tcacgcgcgg tgccgacaat
     1801 gcgctccacc gagccggctt cattgaggac cgggtaggaa cggtcgcgaa cccagcggac
     1861 ctcaccatcg ggccggacaa tccggtattc cacatccgtg acagcacccg tcccctgccg
     1921 gtgtttggtc tgcgcttccg catcccggtc gtcgggatgg atggactcgc ggtaggagtc
     1981 gggtgccgcg tacagttctt ccgggcgacg atgccacaag gtctcatagg ccgggctgat
     2041 gtagagaaag cgtccgctgg caaagtcgcg gttgtaaaac acgtcctgga tgttttccgc
     2101 aagctcccga aagcgcgcct cgctgcccag cagcgccgcc tcggcgcgcg cgcgctcgat
     2161 cgcaatgcgg gccagccgca cggccgtgtc caccaggtcc tggtcggcag gctggggggt
     2221 gtacggcgtg tgatggtaga tggcgaaggt ggccaggaca tcccccgctg cagaaagaac
     2281 cggcgtggac cagcaggact gcaggtcgaa ttcgcgtccg agttcgcggt agttgcgcat
     2341 cagcggatca gcggcccagt gctccaccac gaccggcttt ttgcggaacg ccgccgtccc
     2401 gcaggcgcca acaccttcac tgatctccag cccgtcgatc tgccggctga aggcggccgg
     2461 caggctgagc gccgcgccat ggcgcaggtg agtgccctcg cgcagcaaaa tcgaacacag
     2521 gctgtcggcg gaacggccct cgatcaggcc cacgatggcc tccagggact gcgacaaggg
     2581 cacgccgtag gcaatcagct ccagcacctg ctcctggtca tggaccagct gttcggagaa
     2641 cttgcgctcg gtgatgtcgg taaacaccac cagcccaccc accagctgcc ccgcctcgtc
     2701 gtgcaggggc cggacattgt tgcgcagtac atggtccctg gcgccgggcc ggcgcagcag
     2761 gacatcccag ttgtcgacgc tctcgccgcg aatggcccgg ctcagcggcc ggttctgcgg
     2821 cgcacacacc gtcttgccgt ccagcagcag cacctcgtgg tcggcccgca attgggccag
     2881 cgttttgcct gccacatagc ctggaaaata ctttctcgcg gccgcgttga acagcaggaa
     2941 gcgaccttcg acatcgatgg ccagcacccc ttcactcatg ctgttgatga tcaagtccag
     3001 catgccgcgc tccttgccga tgcgcgccag cgatccgtca ccctgctggc gactcaactc
     3061 cagcgattcg gccatgcggt tgaaggttcg tgcgagatgt ccgagctctc cctggtaaag
     3121 cggactgagc tcaacccggg ccgcgaggtt gccgctggcc agttcgtcgg cttcatgcac
     3181 catggcgttg accgggttga cgatcaggcg tttgcccatg ccccaggcgc aggccatgcc
     3241 gaacaggccg acgaccagca ggacacccag ttccagcgcc agcgctttcg ccggttcagc
     3301 ggcgatcagc gcgcgcgtca tgctcacggc cacaaacagc gcaccgtccg ccgtgccgct
     3361 caccggggca aaggcgtata cacgctgctc cccgccggca tccggcgcct cctcgacgcc
     3421 tacgcgcggg gcttgcaaaa aggcccggat ggttgcatcg gtctgcaggc tgccgaccgc
     3481 gccggcagtt gcaggctgag cggccaggat ggtgcctttg cggtcggtca acaccacgct
     3541 ggcccctgcc gtcggcgcca cctgctccag cagggacgca aaggcctgca cgctgagcgc
     3601 gacaaaaacc accccgctca gcacaccggc gttgtcatgc acgggcgccg caaaaccgat
     3661 gcccggctgg ccgctgcgcc tgcccacgcc atattcgccg attgaaaact ggcggctggc
     3721 catgacccgc ttgaaaaaag gccgggcgct ggcatcggtg cccaccagtg ccgcttcgga
     3781 gtgacacagc acggtgccgt cgagcgccac cacccccaga tcggcgtact gcggatgcat
     3841 cccctgcagg ttgcgcaggt attcgttgca caggccctgc agccggccct tgaccgaggg
     3901 cgcactggcc agatcgccca gaagctgttt gatcgcttcg atttgcggct gctggctggc
     3961 ggctgtcagc agcgcctggg accgcagtgt ggagcgcgcc agcttcaggg catcgctctg
     4021 cttgctggtt gccgtccagg caaacaggcc aagcaccggc agcagcgcca ccacgaccaa
     4081 caacaccaga cgggcgcgaa gccccaacga actgaccatt ccattccttt gcgccttccg
     4141 ctggcccgga gcccttgcgg gccattcttc actgcagacc ctggccccgt gctgcgaagc
     4201 cggcactttg agccctgtgt actttgtacc atcgtagtac tttggcttgc gcatggccac
     4261 ccgctggtca cccgcccgcc gtcctcctac acccggacgc agaacaggct gacaaaacgc
     4321 ctgcgggtga agcagcaaag tggtcgctca atcccttgtt gcaacagaaa gaacgcctgt
     4381 ggccagcccg cttcctgatc cgtccaaaca gcccagcccg tccgcgcgga agcaggccac
     4441 cgaccagccc ggccagagtg agggcatgcc cgtcaagaca ccggcggcag gacgcccgcg
     4501 gcccgggcgc acagaaacgc ctgccgatgc atcggacgaa gccagtctcg cactgccgca
     4561 tgagcgcgac cagtccatcg acatgaccga tgcacaggcc gatccgcaag tgcagcaagc
     4621 cagtcgcgac ctgcgccggg gcctgcagga caccggcaaa agcgttccca tggacaaggc
     4681 ctatgaccgg ctcaagcgct gaagacaggc attctggcgc gggtcactgc tactgatttc
     4741 atagctatat acgcccattg caaaagggct gcagcccgtt ttcatcataa acagagaggc
     4801 gaaggcacgg gcggcgaggc ccggcgcagg gcgtcagggc gtgcgcgacg acacctcggg
     4861 cgcgcccacc ggcacacgct cggtcatcca gcgcagcagg tccagccaca tctggcgcat
     4921 ctcggcgtcc agcggtgtgc gcatcgcatt gggcagttca atggtgacca ccggcacccc
     4981 tttgtgcaca ccaccgtaat tgcccagcga gcccgggaaa ataccgacct ggtccaggta
     5041 cagccgcccc agtttggagg gcggaaccga cggcccgtcg aagtcgagca caccgtaggg
     5101 cgcatgaatg ctgacgatca ggtggggctt gaagcgctgc atctcctgat gcagaaagcg
     5161 cgactccggc tcggacaggg gcttggtgcc cggccagcgg cgcggatcct tgcgggtgcg
     5221 tttttcccag tacaccttgg cgtcacgctc ccagaacggc gtcggaaagt tgcggttcag
     5281 atcaacgccg ttggcgttga cgcggcgtgg cgggctggcc agcagcccgt cgggattgag
     5341 ggccggtatg aaacgccagt gaatggcttg cgaccagggc gccgcggctt cctcggccag
     5401 gcggatccag tgcagggcca ccgaggctga cgacaactcg tcgccatgaa tcgcgcccag
     5461 caccagcaca cgctggcgca catcctcggg cttgatatcg cgcatccaca gggtctgccc
     5521 cttgaccgaa cgggcggccg tgggctgcag ctgggcggcc tcgcagaggt tgcgcctgag
     5581 attcggaagc ttgcgaacaa aatcatcgca aggccccgca gcctgtgcgc cagtcgctat
     5641 caataatgaa gctgccagaa gggccgcggt gcaggttaaa cgcatgccgt gattttacgc
     5701 ggggcattgg ggtaaattag ccccatgacg cctcccactg ccttgcgccg ggccagccac
     5761 cccagttttt caacgcgcga gttccgtgcc tgcctgggca tgtttgccac cggtgtcacg
     5821 atcgtcaccg cgcgcacagc cggcggcgag ctggtcgggc tgacggcgaa ctccttcaac
     5881 tcggtctcgc tggagccgcc gctggtgctc tggagcctgg cgcaggcggc cggctccatg
     5941 ccggcgctga gtaccggctc gcactacgcc atcaacatcc tggcggccga ccagaaagga
     6001 ctggccgagc gttttgcggc caaacgcgaa ggccgctggg agggtgtcgc ctggaccgaa
     6061 ggtgccggcg gctcgccctt gctggccggt gctgcggcca cctttgaatg tttcaaccgc
     6121 agtcgttatg aagaaggcga ccacgtgatt tttgtcggcg aggtcgaacg ttgcagccac
     6181 cgcgccggcg cggcgccgct gctgtttcac ggcggccgct tctacaccga gcatccgctc
     6241 tgacagcacc tgctgcaacc caccgccacg catgacgtca cgcaagaccc atctcgacac
     6301 cctcgccatc tccctgctgc tggcctgctg cctgttctgg ggcttccagc aagtgctggt
     6361 caaggcaacg ctgcccgaag tggcgccggc cttccaggcc gccatccgtt ttgccggcgc
     6421 caccgtgctg ctcgggctgt ggtgcgcctg gcgcggcgtc aagctgttca gcgcggatgg
     6481 ttcgctgcgc gcggggctgc tggcgggctc gctgttcgcg ggcgagtttg cctgcctgta
     6541 tctgggcctg cagtacacca gcgcgtcgcg gctcacggtg tttctctaca cctcgccgtt
     6601 ctgggtggcg gccttgctgc cgatctgggt gaagtccgaa acgctgcggc cgttgcagtg
     6661 ggtgggcctg gcctgcgctt ttgcggcagt ggcctttgcg ttgcgcgagg gtttcagtgc
     6721 cgccggtgcg ccgacctggg tcggtgacgt gatggcgctg gtggccggca tgttgtgggg
     6781 cctgaccacg gtggtcatcc gcgcctcgtc gctggccagg gtctcgccgg aaaaactgct
     6841 gttctaccag gtggccgtca gcaccgtcac cctgccgctg ctgtcgctgg cactgggcga
     6901 gcgctggtcc tggagcttca gcccgtttgc cctcacctcg ctggtgctgc agacggtggt
     6961 cggcgcgttc gccagctacc tcgcctggat gtggatgctg ggccgttatc cggcgaccaa
     7021 aatttccgtc ttcgtgtttt tgacgccgct gtttgcgctg ctgttcggtg cgctgtggct
     7081 gcacgaatcc gccacgccca gcctgctggc ggcgctggtg ctggtggcgc tcggcatcgt
     7141 gctcgtcaac cgcaagccgg cctgagccga cctgcgctcg cagcaagcct caggcggcgt
     7201 tggcgcgctg ctctgcaaac gcaacgtacc agtccaggca accggggttg gccatcgcgt
     7261 cacgattgac caccttgtcg atcggctggc ccagcagcag cttcttgatc ggcaactcct
     7321 gcttcttgcc cgacagcgtg cgcgggatct ccggcacctg gaaaatttcg ttgggcacaa
     7381 aacgcgggga cagcgctgtc ttgatcgcgt tgttgagcct ggccttcatc gcatcgtcca
     7441 gggccacgcc ctcgcgcaac accacaaaca gcggcatgta actttcgcgc cccaggtatt
     7501 ccaggtccac caccatggag tcgatcacct ccggcagcgc ctcgaccgcg ctgtacagct
     7561 cgctggtgcc catgcgcagg ccgtggcggt tgatggtggc gtcggagcgg ccgtagatga
     7621 tgcaactgcc cgagggcgtg atcttgagcc agtcgccgtg gcgccagaca ttggggtaca
     7681 tgtcgaaata actcgagata taacgcgcgt tgccggtatc gccccagaag tacaacggca
     7741 tcgagggaat cggctggctg cagaccagct cgccaacctc gccgatcacc ggctggccct
     7801 gctcgttcca ggcctcgacc gcgcagccga gcaggcggca ctgcatctcg ccaggcacct
     7861 gcggcagctc gcggttgccg cctatgaagg cgcctgcgaa gtctgtgccg ccggaaatat
     7921 tgcaccacca gatgtcctgt tgcgcttcgg ttttggcaat gcgcctgaac tgctccgtgc
     7981 cccaggtctg cgcctcgggc gacagcggtg agccggtggt gcccagcgcg cgcacggacg
     8041 acaggtcgcc gcaggtcgac aggtccacgc cggccttcag gcagttggca aaaaaggccg
     8101 cgccggcgcc gaagaaggtc acgcccagtt tcgcgccgaa gcgccacaag gtggtccagt
     8161 ccggcttgtc cttgccgcca ccggggttgc cgtcgtaaat gcagcaggtc gtgccgttga
     8221 gcaggccgct gacctgggcg ttccacatca cccagccggt ggagctgtac cagtggtaac
     8281 gctcaccgaa agagtttgct tcgtagctgc agccgatgtc gttgtgcagg gtcttgagcg
     8341 ccagcgccac cagcacggtg ccgccatggc cgtgcacgat gggtttgggc aggccggtgg
     8401 tgccgctgga atagacgatc cacagcggat ggtcgaacgg cagccagagc ggttcaaacg
     8461 ccgaagtttc ggcatcattt cgggctgtag cgctttcaaa ccgtgcgctg tcagctatgg
     8521 aattagtagc atcgaggttg tagtggacga tcacgtgctg cagcgaaggc agggccgcac
     8581 ggatctccgc caccaccgcc gtgcggtcga tgtcgcgccc gccgtaagtg acgccgtcgc
     8641 aggcaatcag caccttgggt tcgatctgcc ggaagcggtc cagcacggca ttcgtgccca
     8701 tgtcgggcgc gcagatgctc cacacgccgc ctatgctgac caccgcgagg aaagccacca
     8761 tggcctcggg aatgttgggc aggtaagcgg ccacgcggtc gcccggcaac acaccctggg
     8821 cttgcaggtg cagcgccagc gacgccacct ggcggcgcaa ctcgggccag ctcagctcgc
     8881 gcggcggcgc gctgtcgagg cttttttcgt tgtggctgag cagcgcggga aagcccgccg
     8941 catgggcgcg atcaacgtgc cggaacactt gctgcgcgta gttgaattgc accccgggaa
     9001 accagcgggt cccgggcatt tttgcctcga ccagcacgtc ggtagcgggc gtgggcgatt
     9061 gcagatcaaa gtagtcccag atgctttgcc agaaagcgtc caggtccgtc accgaccagc
     9121 gccacaaggc gtcgtaattg tcaaaagaca gctggcgcgt gtcgcgcagc cagttctggt
     9181 acaggcggat ttgggggatg tagggggcgc tcatggggcg aaagcttagc aggccacccc
     9241 tcgcgcaagc atccaccttg gcgacaggcg cggggggtac cgccgtcgca aaagcaaaag
     9301 gccgcccgca ggcagccttg gggcgggtca ggacccgcta cagggccgca tcaggcgtgc
     9361 gtgacgcccg atgcaaccgc cgcaagacac ctcccttact tgggcgtgcc catcatcatt
     9421 tcgacgtcgg tcatgggcac catgccgttt ttggccggct tcatgctttt ccacatggcg
     9481 gtttgatagc ggttccactc cctcgaggaa accatgccgt cgccgttggt gtccatgctg
     9541 ttcatggcgg tgttgttgag cttggcgtcc accttcatct cggccttggc ggcgggcgtg
     9601 ccaccggcgg cggaggggtt gatctgcgca ttggcagcag cgccgccgga accggccacg
     9661 gggttttggg cggacagcgt ggaaccattg gccttcttgg tgtcgacgtt catctcggcc
     9721 ttggccgcag ctttgccgcc cgatgcggac gggttgatct gcgcgttggc agcagcgccg
     9781 ccggaaccgg ccacggggtt ttgtgcagac agggtggaac cattggcctt tttggcgtca
     9841 acgttcatct cggccttggc cgcaggtgtg ccgccggaag tggtcgggct ggcttgtgcg
     9901 gaaggcgtgg caaccacgcc agcgggtgcc tgggcaaagg cagcggtaca ggccagggcg
     9961 gccagggtca ggacaaggga ttgctttttc atgggggctc caaaaatgta agagcgatgt
    10021 gctcaaagtc atctttgtct ggccggcata cccccgctgt aggatgaaca ccgtggtgac
    10081 cgtcagggtc tgtcggctcc cgggcgtcgc gaatcagtca aaaatgtgct cggcaacata
    10141 aatcgcacag tcgtgctaat atttgcgcca tggacgccaa aacccagaaa agcgagatga
    10201 cccgcgccgc cattgtcggt gcggcgctgg acctcgcgtc cgcagaggga ctggaggcca
    10261 tcacgctcca ggctgtggcc gaccgccttg gtctgtccaa gagcggggtg ttttcacgca
    10321 tcggttcgcg cgaggccctg cagaaagcgg tgatcgacga gttcggaaga cgatttctgg
    10381 ccgatgtgtt tgtccccgcc gtgcagcagc ccaaaggcct gccgcggctg aatgccattg
    10441 tgcagcgctg gattgtgcgc acgcgggacg tggaggcgca taccggctgc gtgtacagcg
    10501 ccggtgcctt tgagctggac gaccgcgaag gcccgctgcg cgaccacctg cacggcgaga
    10561 tcacgcgctg gcgcgccgcc ctgcgccgca ccatcgtgca ggccgtcgag aagggcgacc
    10621 tccggtccga taccgacccc gagcagctcg tggccgaaat ctacgccctg atgctgggcc
    10681 tgatccacga cacgcgcttc ctgcgcgact cccgcgccgc agaacgtgcg caggccagtt
    10741 ggcagcgcct ggccagaagt taccagccat gagttttgtc agccccacaa gggctttttg
    10801 ttggtcaatg tttcgcacaa ccgtgcgaag aaagggaaat gccatgttct tcctgtttgc
    10861 agtcatcgcc gtgtttgccg gcgcccatgc ggttcgcacc gccctcgcct cgctgcgctc
    10921 cctgccgcgc agcaatgaag actggatctg gtactgatcc ggtaaagcac caaggagcct
    10981 ttcatgacac gtacatcact tgaagccact gccgcctact acaacgccag tcccgtgacc
    11041 cggctgttcc gctggggcct gggcgcctcg cagcgcctct ggccggcgct ggccgtgcgc
    11101 gccgcctacc gcctgttcgg cacgccgctg ccgccccgct ggctgggccg ccggcgcgcc
    11161 tgggcgccgg actggcgcat cagcagctgg tccttcgaaa acgccagcct gacgatttat
    11221 gaacgcccgg cggcaccgca tggcccgctg gtgctgctgg tgcatggctg gggcgggcac
    11281 gccggccaga tgctggcact ggccgacaca ctgcaggcgc agggcctgcg gccggtgatt
    11341 cttgaaatgc cggcccatgg ccgcagtagc ggatcgacca gcaacctgcc gcagttcgcg
    11401 cgcgccatcg actacaccgt ggcgcgcctg cagcaggcgg ggcaccaggt gcaggcgctg
    11461 gtcgcgcatt cgctggccgc caatgccggc gcctacgcgg ccagccgcgg cctgccggtc
    11521 ggcaagctgg tgctgctggc gcccccggcc tcgccgcatg aatacacgcg gctgtttgca
    11581 catgtgttcg gcctgcgcga gtccacacgt gcggccatgc agcggcgcat tgaagcgcgc
    11641 gaaggcatcc tgatgccgca gttcgaaccc gccgccgtcg gcccgcgcat cgcacagccc
    11701 acgctggtgg tgcatgaccg caatgacagc atcaaccgct ttgccgacgg cgtggcctac
    11761 agccaggcca tcaccggcgc ccggctgctg gccacgcagg atctcgggca ccggcacatc
    11821 ctgaaagacg ccggcgtgct gcgcgaactc gccgcctttc tggtctgaaa cgcgcgactg
    11881 cgccgcaaca ccccttgtgc ggcatgccca ttgcttctag gatggcgcca cttacaagga
    11941 gcggttgatg ggaaattcaa aagggttgtt cagctggaca gaaaaaagca gcgcggtatt
    12001 gcaacagggc ggcgtgatcg gccccgatga acgcctgccc tggccgcaaa cggcggtgat
    12061 gggcgtgcag cacgtgattg cgatgttcgg cgccacggtg ctggcaccca tcctgatggg
    12121 gttcgatccg aacatcgcca tcctgatgag cggcatcggc acgctgatct ttttcctgat
    12181 caccggcggc aaggtgccga gttacctcgg ctcgagtttt gccttcatcg gcgtggtgat
    12241 cgccgccacc gcctacgccg gcaaggggcc caaccccaac atcggcgtgg cactcggcgg
    12301 catcattgcc tgcggcgtgt tgtacacggt tattggcgcc atcgtgcagg ccgtcggcac
    12361 cggctggatc gaacgtttca tgccgccggt ggtcaccggc gccgtggtgg cggtgatcgg
    12421 cctgaatctg gccggcatcc cgatcaagaa catggccgcc agcaactttg attcgtggat
    12481 gcaggtcgtg acctttgtca gtgtggcact ggtcgcagtg atgacacgcg gcatggtgca
    12541 gcgcctcttg atcctggtcg gcctgatcgt cgcgacgctc atttatgcgg cgctcaccaa
    12601 cggcatgggg ctgggcaagc cggtggatct gtcgccgatt gccaatgccg cctggttcgg
    12661 cctgcccggc tttgccgccc cggtgttcag cgccaatgcc atgctgctga ttgcgccggt
    12721 ggccatcatc ctggtcgcgg aaaacctcgg tcacatcaaa gcggtgacgg ccatgaccgg
    12781 caagaacctc gaccagtaca tggggcgcgc cttcatcggc gacggcgtgg ccaccatggt
    12841 ctcgggcgct tcgggcggca ccggtgtcac cacctatgcc gaaaacatcg gcgtgatggc
    12901 cgcgaccaag atctattcga cggcggtgtt tgtggtcgcc ggcctgatcg cggtggtgct
    12961 gggcttcagc cccaagttcg gcgcgctgat ccaggcgatt ccgctgccgg tgatgggcgg
    13021 cgtcagcatc gtggtgttcg gcctgattgc ggtggccggc gccaagatct gggtcgacaa
    13081 caaggtcgac ttttccgaca acaagaacct gctggtggcg gccatcacgc tggtgctggg
    13141 caccggcgac ttcacgctga agttcggcca gtttgcgctg ggcggcatag gcaccgccac
    13201 ctttggcgcc atcctgctgt acgccctgct caatcgcagg aagtaaaacc aggcagcccg
    13261 tgccccgcct gcgcggggcc tatttcgcct gcgccttggc cagcgcggcc ttggtggtgg
    13321 ccagctcgcg ttccacatcg gcacgttcgc cgatttcctg ctccagcagc tcgttgacat
    13381 gcgcgaggtc ggcggcggac tcctggatct tgccctccag ctcgcctgtt ttctccagcg
    13441 cctgcgccac atcgccggtc tggatatggt cggggatttc ctgcttgagt accgcgttga
    13501 tcacaaacag ctcggcgctg gactgtttca cggtttcctc gacagtttcg ttccggtcca
    13561 gcgcacgtgc cagtggcgtg tctgcggaag gggcggactg cggggacggc tgggatggct
    13621 tggacatggt gagcacctgg agggaaacgg gaatgatctg gcgacaaacg gcgcccgggg
    13681 gcacgcaagg ccgcccgacc cctgaagccc ctatggtaac caagtgccct tcccggggcc
    13741 agcgatgtcc ccaccgccat caggcctcag ggcaagctgg aaaaggcggg ccggttcggg
    13801 aggatggcgc tcagggcgcc gttcccggcg ttgcgggtgc gggcgtaccg ggctggcggt
    13861 ccggcccgga attgaaaatc ctgcccaggg tggcgcccag acgcgcaccg atggccgtgc
    13921 cgatgccggg cagcaccgcc gtgcccagca gcgcgccggc cacagcaccc ttcggcaccg
    13981 acacggccac cttgtccacc ggcccgctga gcaccagcgg caccccgacg atgccgtcca
    14041 ccagatccac cgcaaattcg gcctcgatct gccggttggc cagccgggcc tggccggacg
    14101 cactcagggc gcccgagctg gccttgatcc cggtgaattg ggtcaccatg ccgcgcggcg
    14161 tgttctgggt gtcgagctgg ccggacaccg cgtcgagcgg cgtctgcccc gcatgttcct
    14221 tgccgaggct gcgcacggcc cggtccagat cgaagcgcag cagggttgaa gcgcccatgg
    14281 tgaacacggt ttttgtatgc agcgactgcg ccagtccgac caggctgtcc ccctgggccg
    14341 acagcacggt gtttcccgtg gccttgccgg caatgaccgg cttgcggttg aaggcggcca
    14401 ccgcgctcgc cacgtcaacc ccgctgggct gcaattgacc ggccagcacc aggcggccgt
    14461 cggcacgggc ctgcaactga acctcgccat gggccgtgcc gccgccgacg ttgatacgtg
    14521 tggcccagcg gtcttcctgt ccttgccggg tcagggtcag atcggtggcc ggcgtgaagc
    14581 cggggcgccg caacacggcc tggcggggtc gccagcccgg gtcaaagtcg atctcgccgt
    14641 cgtacaccac cgccacgcca tggcgcgaca gccaggtcag gtcgcgaaac acaaaacgcg
    14701 ccagcggtat ggccacagct tccacggttt ccacagcttc cgcagggacg ccgcgtgacg
    14761 tcgcgtccag cacccgcaag gacagttgcg gcacgaccgc accatcgagt tccacgcggt
    14821 ccaggatcac acgccgctgc agcagggcca gcaggtccgg ataggcggcg agctttttga
    14881 ccgtgatggc ctgcggctgg tcggtcgcca cgtcctccag cacgatggcc ggtaccggca
    14941 acagacgcca gtgcagtgcg cccagccgca ccggcacgcc aagccgggcc tgcagctcac
    15001 cggcggcgtg ggcggccaga tcctcgtcac tggggatgaa ccacagcagc gccagccaca
    15061 ccgcgcccag cagcagcacc aggccacctg cggccaccat ccatgtttgt ctgcgtgcca
    15121 tgctgccttc cgccgttgtt gcaccgtctg ccatctgcat ctgcatctgc catccacctt
    15181 cctgagctta agaggctggc cgcgcggcac ttgtaggccg caatctcttt atagtctggg
    15241 ttgcataccc ttttgacgcc ctgtggaacc acgatgccta tctggaaaca accgatttct
    15301 gtcgaactgc tgaccctcaa caacgccaac agcgccgtga cgcacctggg catcgaattc
    15361 ctggaggtcg gcgacgattt catccgggcc cgcgtgccgg tcgatgaacg cacgcgccag
    15421 ccctatggcc tgctgcacgg cggggtgtcg gtggtgctgg ctgaaacgct gggctcctgc
    15481 ggcgccgcct ttgcagcgcc cgaaggccac cgcacggtgg gcctggacat caacgccaac
    15541 catctcaagg gcgtcaccag cggctgggtc atcgggacca cccgaccggt gcacatcggc
    15601 cgcagcaccc aggtctgggc catcgagttg accaacgagg ccggggagct gacctgcgtc
    15661 tcgcggatca cgatggcgat gctggccccg cgtcaagagt aaaatcgggc tccagccctt
    15721 atcggacggg cgcaaacagc tattaatacg atagcaaatg agcgtttcag tccgcggccg
    15781 tccgggccat gcgttcgctc ttccagtaca cgtcgtcgcc gccgttcatg cggttgagca
    15841 cgcgcgacag cacgaacagc aggtcgctca gacggttcag gtactggcgt ggcgactcgt
    15901 tcagcgtctc ggccttgtcc agggccacca ccgcgcgttc ggcgcgcctg gccacggtac
    15961 ggcacacatg cgccagcgag gcagcacgcg aaccggccgg caggatgaac tcctgcaggc
    16021 gcggcagggt cgcgttgtat ttttcgagcg ccgcatcaag cagcgccacc gcttcgggct
    16081 tgagcagttc aaaaccggga atcgacagct caccgcccag gttgaagagc tggtgctgca
    16141 cctcgaccag cagttcgcgc acgtcggccg gcatctcttc acacagcaac acgccgatct
    16201 gcgagttcag ttcgtcgacc tcgcccatcg catgtatgcg caggctgtcc ttggacacgc
    16261 gggtgttgtc accgagcccg gtcgtgccgt tgtcgccggt gcgggtggcg atctgagtga
    16321 gtcggttggc catggtaaat atccggcagg ttacgccggg aaagaagatg ttgtggagcg
    16381 accgatgatg tcaatgaggc aacagcgtgc aagagtgctg gccggcgcgg gctccctgcg
    16441 ctgcaatgac agcttcttca acttcaagga cctgtctcga tgacgtctgc ttttcatttt
    16501 attcccaact tcgaaatccg cagtgttgcg ctgctcgccg cgctgaccgt cggcgccgcg
    16561 ctgggtgcgc atgcccaggg cgccgacagc ggcggcatga aaacaccccg tggcgcgggg
    16621 acgcccgcca cccagggcac accctcgccg tccggcccca ccaaccagga ggtgggcgct
    16681 gccttcatcc gtgccgacgc caacaaggac ggcaagctca gccggcagga ggctgtgcgt
    16741 tttccagccg tggaacagcg ttttgacaag atcgacacca acaaggacaa actggtgtcg
    16801 cgcgaagagt ttgaaatcgc gctgaagtcc tgacggaggt ggccagagag cccgtggggc
    16861 gccggatggc cccccatggg ctagtgactc gtcgtaaact ggggcttccg accggagaca
    16921 ccatgaacgc ccccacccat cccgaccacc tgctgcccca actcaatctg cgcgctgtgc
    16981 cgcaggccct gatcgaggcc ctgaaagaac ggtttgccga gcgctgctcc accgccctcg
    17041 tggtacgtga gcagcacggc cgcgacgagt cctcgtttgc ggcgccgccg ccagcggccg
    17101 tggtgtttgc cgaaagcacg gccgacgtgg ccgatgcggt caagctggcc gcccagtacc
    17161 aggtgccggt cattcccttt ggtgtcggca cctcgctcga aggccatctg ctggccgtgc
    17221 agggcggcat cagcattgac gtgagccgca tgaacaaggt gttgtccatc aatgccgagg
    17281 acctgaccgt gaccgtgcag gccggtgtca cgcgcaaaca actcaacgaa gaggtcaaga
    17341 gcaccggcct gttcttcccc attgaccccg gcgccgacgc cacgataggc ggcatgagcg
    17401 cgacgcgcgc cagcggcacc aacgccgtgc gttacggcac catgcgcgaa aacgtgctgg
    17461 cgctggaagt ggtcaccgcc agcggcgaca tcatccgcac cggcacccgc gccaagaagt
    17521 catcggccgg ctacgacctc acgcgcctga tggtgggcag cgaaggc
//
LOCUS       DAIDFG010000027        17313 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_27_length_17313_cov_18.399583, whole genome shotgun sequence.
ACCESSION   DAIDFG010000027 DAIDFG010000000
VERSION     DAIDFG010000027.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 17313)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17313)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17313
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27_length_17313_cov_18.399583"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3055 a         5350 c         5766 g         3142 t
ORIGIN      
        1 ctgccgtgtg tgggcccgcg gtgggcatag gcaccacgct gctgctgcac tgcgatctgg
       61 tttatgccgg cgataacgct gcgttttcca tgccttttgt gaacctgggc ctgtgcccgg
      121 aagcggcgtc gagctttctg gcaccgcagc tgatgggtta cgcacgcgcg gccgaggcgc
      181 tgctgctggg tgaacccttc atggccgagt cggcgctcga gatgggcctg atcaaccgca
      241 tcgtgccacc cgccgaagcc aacgcgctgg cgcagcgcca ggcgcaaaaa ctcgcggcca
      301 aaccgctgag ctcgctggtg gaaaccaagc gactgatgaa aaaaggccag gccggaatgg
      361 tggccgagcg catggccgaa gaaggggtga gttttggccg catgctcaag gagccggccg
      421 cccgcgaagc tttcggcgcc ttcatgcaaa agcgcaagcc ggatttctcc ggggtttgag
      481 gcaaaactgg gcttgatccc ttatccagca agcgcaggca gctataaatt gtgtagcact
      541 caggaggcat catgagttca gttcccggtc tcaaaggcaa aaccctgttc atcaccggcg
      601 cctcgcgtgg catcggcctg gcgatcgcca cccgtgccgc ggccgacggc gccaacatcg
      661 tgatcctggc caaaaccacc gaccccaacc ccaagctgcc gggcacgatt tacagcgcgg
      721 ccgaagcggt gaaagctgcg ggcgggcagg cgctgccgct ggcggtggat atccgtgacg
      781 aagaggccgt ggcggccgca gtggccaagg cggtcgaggc tttcggcggc atcgacatcc
      841 tggtcaacaa cgccagcgcc atcagcctga ccggcaccga acacacgccg atgaagcgtt
      901 atgacctgat gaacggcatc aatgcgcgcg gcacctacct gtgcacccag gcttgtctgg
      961 cggagttgaa gaaatccgcg ctggccgggc gcaagccgca ggtactcacc atgtcgccgc
     1021 cgctgagcat gaagcaacac tggttcgaga accacacggc ctacaccatg gccaagtacg
     1081 gcatgagcat gtgcaccctg ggacattccg gggaattcag gaaatacggc attgccgtca
     1141 acagcctgtg gccgcgcacc gcgattgcca cggccgccct tcagatgatt cccggtgtgg
     1201 acctgaagct gtgccgcaag ccggagatcc tttccgatgc ggcccatctg atcctcacca
     1261 gcacgggcat gcaaaccggc aacttttata ttgacgacga attgctggcc gcgcatggcg
     1321 tgacggacct tgagaagtat tcggtaacgc cgggcacaaa gaatttcatc cctgattttt
     1381 tcgtggactg aaccatgccc tacatgcttc tcatccacga acccgtcggc cagcgcctga
     1441 cccgcagcca ggcggaaggc gaggcggtgt acgggcgcat gctcaagttt gccgacgagc
     1501 tgaaatcaga aggtgtgttg ctgggcgtgg agtcgctggg ctcgcaggac aacgccgccc
     1561 gcgtgcgtgt gagtgccggc aagccgcagg tgctggatgg accgtttgcc gaagccaagg
     1621 aaatggtggg cggcttcttt ctggtgaact gcgcaacccg ggagcaagcc ctggcgattg
     1681 ccgcccgctg ccccgccgct gaatgggcca cgctggaggt tcgcgccacc ggcccttgct
     1741 tcctctgagc cccggcagca gcaggtaagg gtttttacct ggttctggct tgtcgatttc
     1801 gcatcggctg cttcgtcgtg tcaacaggag gccagggatt ctggctgccg tccacaagga
     1861 gtacccccat gcgattcatg atcattgtga agtcgacgcc cgagttcgag gccgagacca
     1921 ccccgcagac ccccgacgaa aaactgatgg ctgccatggc cgacttccac gaggagttgg
     1981 cccaagccgg cgtgctgctc gatgccagtg ggctgcagcc cagccgcaag ggctggcgca
     2041 tccattacga cggccagaag cgcaccgtga ttgacggtcc tttcgcggaa gccaaggaac
     2101 tgatcgccgg cttcaccacc atccaggtgc gcagccgcga ggaggcgctg gagtggtcgc
     2161 gccgttttcc caatcctgcc ggcgaaaacg cgccggccgt gatcgaggtt cgccagctgt
     2221 atgaactgga cgacttcgag cccagcgagc aggtcgagcg tttccgcgag atgaacacgc
     2281 ccagccagaa gtaaggagcg cgccatgatc aaaaccacct tgctcatcct gctggggctg
     2341 gtcgtgctgg ccatcgctgt cgtcgcggtc atagctttgc aacgacccga cactttccgt
     2401 gtgtctcgca gcaccagcat cgctgcgccg gcagaaaaaa tcttcccgct ggtcaacgac
     2461 gtccgggcct tcaatacctg gaacccgtat gcgctgaaag ccccggagat gaaaggcagc
     2521 tacagcggcc cgttcatcgg gcccggcgcc cactacgact tcgaaagcaa aaagtcaggc
     2581 agcggcagct ttgaaatcgt gcgggccatg gcgccttcgc aggtacagat gcggctcgac
     2641 atgaccgcac ccttcaagac caacaacctg atcacctttt cgctgacacc cggaggtcag
     2701 accacacagg tcacctgggc catggaaggc cccgcacctt ttgtctccag attcatgggt
     2761 gtgattttca acatggacaa gatgatcggt gccgacttcg aagccggcct tgccaacctc
     2821 aaggccaaag cagaaaaagc ctgagagccg ccgccttatt gaaaccatct tcaaaggagc
     2881 aaccatgtca cgccagattt tcgtcaactt gcccatcaag aacatggaaa ggagccaggc
     2941 cttcttcaaa agcctgggct tttccttcaa cccgcagttc accaacgagc agggcgcctg
     3001 catggtgatc agcgaggacc acaactacgt gatgttgctg gtcgagccct tctttcaggg
     3061 cttcaccaag aagccggtcg ccgatgccac gaagaccacc gaggtgctgg tcgcgctgtc
     3121 gtgcgggagc cgcgccgagg tggacgagat ggtgagcaag gcgctggccg ccggcggcac
     3181 cgcgcccaat ccgtcgcagg accatggctt catgtacagc catggtttca ccgacctgga
     3241 cggccacgtc tgggaggtgt tctggatgtc taattgagcc cccacggtcg ctcactgcgt
     3301 gtagctccct gcccccccga gggggccgct gcgcctgcgg gccggcaaag ccggacccgc
     3361 ggccccctgc ttgattgaat accaggcttg cgctccacct gagcatctgc tctgccgcta
     3421 ctaccattga tacatgcatt cctccaccca tgaggcgatt tctgccgtct ggcgcatcga
     3481 gtccgcgaaa atcgtggcct cggtggcgcg tttggtgcgc gagatcgggc tggccgagga
     3541 actggcgcag gacgcgctgg ttgccgcgct ggaaagctgg cccggcaagg gtgtgccgga
     3601 caatcccggc gcctggctga tgcgggtggc caaaaaccgt gcgctggacc acctgcggca
     3661 ccggaagatg cagggcgaaa agttggcgca gatcggccat gagctggagg cgcaggaagc
     3721 gctggtcgtg ccggatttcg tggacgcgct cgacgaggcc cggcaggacg agatcggcga
     3781 cgacctgctg cgtttgatgt tcaccgcctg ccacccggtg ctttccaccg aggcacggct
     3841 ggcgctgacg ctcaagctgc tgggcggcct gaccacccat gaaatcgccc gcgctttcct
     3901 ggtacccgag cctacgatgg cccagcgcat cgtgcgtgcc aagcgcaccc tggccgaggc
     3961 gcgcgtgcct ttcgaagttc cgcgcggcga tgaactgggc gcacgcctgg cctcggtgct
     4021 ggaggtggtc tacctgctgt tcaacgaggg ttacaccgcc accgccggtg atcactggat
     4081 gcggcctgcg ctgaccgacg aggcgctgcg cctgggccgc atgctggccg gcctggcaac
     4141 ggacgagtcc gaggtgcagg gtttgctggc cctgatggag ttgcaggcct cgcgcatggc
     4201 cgcccgggtg gatgccgccg gccggccgat actgctggcc gaccagaacc gggcgcgctg
     4261 ggaccatctc ctgatccgca gggggctggc cgctctcgac atggcgctgg cgctggccga
     4321 gtcgtcggcc cgcgccctgg gaccctatgc gctgcaggcg gccatcgccg cctgtcatgc
     4381 gcgcgcgccg acttcagcag agaccgactg ggtgcgtatt gccgccctgt acgacgcgct
     4441 ggcccaaacc gcaccatcgc cggtggtgga actgaaccgc gccgttgcgg tgagcatggc
     4501 cttcggaccg gcagccgggc tggccatcat cgatgtgctg cgcggcgaca aggccctgca
     4561 gaactaccag tggctgccca gcgtgcgcgg cgacctgctc gccaggctgg ggcgcaacga
     4621 cgaggcgcgc catgagtttg aacgcgccgc ggcgctggcg ggcaatctgc gtgagcgcga
     4681 attgctgctc gagcgtgcgc atcaactgga cggcagctgt tcgcctggtg ctgccggata
     4741 gacctcggac acggttgtgt gcttggaaag tcctgccggc cagcgggttc cgtcgacacc
     4801 tccgccaggg gtaggcgcca gcctacaagc tgatcgggcg ggctgacagg ccccggccca
     4861 cccgcctacc ggacaacagc gccgcaagct acttgcggcg gctgcctgcg cacgcagtat
     4921 cgagtcattg catcaccacg gaagggtggc cgccatgacc aagttatcga tcgcgggttt
     4981 caatgccatg cctgaagggc gcaaaccaca gacgctgcgc atccctgcgc ccccactgcg
     5041 cctgcccagg cacctgctgg aacatctggc ccggccgctc aacaacgcgg acgatgtaga
     5101 ggcgctgatg gcgcacatcc gggtggccga acaacggggt ttgctctgag cgcggcatga
     5161 ctcaggcgtc gagcagcgcg aggtaggccc ggcgcgtttc tggtgcgacg gacgccagca
     5221 cctgctcata agacgtcttg tgtcggacca gcagccgggc agaggcgacg gccttggact
     5281 tgcagaacca gtggcagctg tgctgcatca ggaaaacctc ggcggacagg gtgaaggcct
     5341 tgtttttggg cgattgtccg gacgtgtttt tcgcggcgcg cgtgatgccc tcggcatgca
     5401 ccagcaggac ctggcgcaag tcgaaggtcg cagccggaaa gagcttgtcg gcgtaaggga
     5461 aggccaggga gagcttgctc acgcgtgtcg cggcgccttc cacccgggag aacccggcca
     5521 cgaagttgtt gaactgctcg cacagcctgg tttcggtgct cgtcaacagg ttccagatgc
     5581 ttgcgcggcg ctccggatcg tcctcgcgaa gtgcacgcat gtagccttcg gtcaggcttt
     5641 ccatgagttt ttcgatctgg tactggccga gatggctgcc cagcaaggcg atgcgcttgc
     5701 gttcgtagcc cgatttgagc gcataagcgc cggcggcgac gatcagcgcc agaaagaaaa
     5761 tgtccatcaa tgcttcagtt ttgttttggg gaggtcccca gtgtagtcag ctggggttgc
     5821 ggggtcggac cgcagccagt gtctattttt acaaagcaag cgcttgctaa aaataacggc
     5881 ttttgttatg gtctgtttcc atggacaaaa cacctgtgcg cacccgccct gccggccggg
     5941 tcttggccct gccagcagcg gttgtagccg ccgctgaaaa gcggccgcgt ggccggccgc
     6001 gcaagacgcc cggcgaacgc gacgacggca accggcgggt ggaactggtc aacaccgccg
     6061 cgcggctgtt tcgccgcaag ggctttgacg gcaccagcac gcgtgatatc gcggcagccg
     6121 ttggcatgca cagcggctcg cctttctacc atttcaaaag caagggcgcg ctgctttatg
     6181 cggtgatgga ggagggcatg cgttcggcca tggcccggca ggcagcggcg ctggaggcgg
     6241 cggcgccgtc tgcacccgac gcggcggctt tgctgcacgt gttgatccgc aatcatttcg
     6301 acgtgctgct gggccccggc agcgatttca ttcccgtgat gctgtacgag tcgcgttcga
     6361 tcacggccag gcaacgtgcc tcgctggcca ggctgcaggg cgactacgag tcggtctggg
     6421 cgccggtgct gcaggccctg agcgaccagg gccggctgca ggccccggtg aagctggcgc
     6481 ggctgctgat ttttggtgcg ctgaactggt cggcgcagtg gtttgacgca aaaaaagggg
     6541 cgtcgctcga tgaactggcc gacgcggcca tggcactgtt catcggcgac aaccggacgc
     6601 ccgctacaag gaaagcccga gcatgactta ccgttccgtc ttcagcgccg gcctgtttgc
     6661 cggcaaggtg gtggtggtca ccggtggcgg ctccggcata ggccgctgca cggcgcatga
     6721 gctggcggcc ctgggcgcgc atgtcgtgct gatcggccgc aagctcgaga agctgtacga
     6781 ggtggccggc gagattgtgg ccgccggcgg ccgcgccagc tttcacccct gcgacatccg
     6841 ccaggaagcc gccgtgcaga cgacggtcgc ggcgattgtg atcgcgcagg gccgcatcga
     6901 cgcgctggtc aacaacgcgg gcgggcagta catcgcgccg ctggagagca tcagcgccaa
     6961 gggctgggaa gcggtcatca acaccaacct gacgggcggc tttctgatgg cgcgcgagtg
     7021 ttatctgcag tccatggcgg agcacggcgg cagcatcgtc aacatcgtgg ccgacatctg
     7081 gggctccatg ccgtccatgg gccacagcgg tgcggcgcgg gccggcatgg tcagcttcac
     7141 cgaaaccgct gcggccgaat gggcgcacag cggcgtgcgg gtcaatgcgg tggcgccggg
     7201 ttacatcgca tcgagcggca tggaccacta tccgcctgag gccggggaga tgctgcgcga
     7261 gatgcgcacc accgtgccgc tgggccgctt cggcaccgag gcggaaacct cggccgccat
     7321 tgtgtttttg ctgagcccgg cggccagttt catcagcggc accgtgttgc gggtggatgg
     7381 cgcgcggcca caggtgcgca tgggctggcc catgcgcctg ccagatgacc aggcgcagca
     7441 gcgcgatgcc gtgaaacctt ttgacggttt tcatctggcc accaccccga aggtgttcca
     7501 gtcatgactg ttttccagtc ggcctggaac gcgcagggca gcgtggcctt gcggcggcgc
     7561 gacgccatgc tcgcacgcat cgcggcgctg cgtgcgctgg aagcccgtgc cgcacaggct
     7621 tcggccaaat cgaaaccggt gttcgacaag cgcggccagc tgttgccgcg tgaacgggtg
     7681 gcgctgctgc tggacccggg cgcgccgtac ttgccgttgt gttcgctggc ggggttcatg
     7741 caggaccaca aggacccggt gctgtccgtg cccggaggcg gcatcgtggc gggcatcggg
     7801 ttcatcgagg gcgcgcgctg catggtggtg gccagcgact cgggcatcga ggccggcgcg
     7861 atccaggcca tggggctgga gaaaatcctg cgcgtgcagg agatcgcgct gcagaacaag
     7921 ctgcccttca ttcacctggt ggagagcgcc ggcgccaacc tgatgcgtta ccgcgtcgag
     7981 gggtttgtcc acggcggctc cctgttccgc aacctcgcgc gcctgtcggc ggcgggtatt
     8041 ccggtcgtca ccgtgcagca cggctccggc acggcgggcg gggcctacat gccggggctc
     8101 agtgatgtgg tgatcatggt gcgcaaccgc tcgcgcgcct ttctggccgg accgcccctg
     8161 ctgatggccg ccaccggcga ggtcgccacc gaagaggaac tgggcggcgc cgagatgcac
     8221 accggcgtgt cgggcctcgg tgaacagctg gccgaggatg accgccaggc gctgggcctg
     8281 gcgcgccagg tcgtggcccg gactgcatgg tcaaatcgac ctccagccca ggcaggacgt
     8341 gcgcaagcag ctatggattc agtagcaaga gagcctttgt tcccggccga tgagctgctc
     8401 gggctgatgg ccggcgatct gcgccagccg gtggacatgc atgaagtgat ggcccggctg
     8461 gtcgatggct cggactttct gcccttcaag gccttgtacg gcgccgccac ggtgtgtgtg
     8521 caggcggcga tcggcggcca cgcggtgggc atcatcagca acaacggccc catcgatgtg
     8581 gcaggcgcca acaaggccac ccatttcatc cagctgatgt gccagctcgg ccatcccatc
     8641 gtctacctgc agaacaccac cggctacatg gtcggcaagg acgccgagca gggcggcatg
     8701 atcaaacacg gctccaagat gatccaggcg gtcaccaacg ccacggtgcc gcagatcacc
     8761 atccagtgcg gcgcctcgtt tggtgccggc aactacggca tgtgcggtcg cggttatgcg
     8821 ccgcgcttcc tgttcagctg gccgagtgcc aaaaccgcgg tcatgggcgg cgagcaggca
     8881 gcgcgcacca tgcagatcgt gacggaggcc gcgatggtgc gtaaaggcat cccagtcgat
     8941 gcggcgcagt cgcaggccca gttcgacaag atcgtcgccg tgttcgaggc gcaggccgat
     9001 gccttctaca cctcgggcct gctgctcgac gacggtgtga ttgacccgcg cgatacccgg
     9061 gcggtgctgg cgcagtgcct ggcgatgtgt gccgaggccg acacccggca actgcagccc
     9121 atgcagtttg gagtcgcgcg aatgtaaaac cccgcttgtc attgcgggct cgacccgcaa
     9181 tccatgcggg cgcaccgtca tggatcccgg atcaggtccg ggatgacgac ccataaaagg
     9241 agacaaaaat gcaatacacc cacgagcacc tcgaaatcca gaaaaccctc aaacgtttta
     9301 tcgacgccga gatcaacccg catgtggacg aatgggaggc tgccgaaatt ttcccggccc
     9361 atgaggtgtt caggaaactc ggcaagctgg gcctgctcgg tctgaccaag cccgaggaat
     9421 acggcggcgc aggactcgac tactcctacg gcgtcgccat ggccgaggcg ctgggccaca
     9481 tcgattgcgg cggcatcccc atggccatcg gcgtgcagac cgacatgtgc acgccggcgc
     9541 tggcgcgttt cggcagcgac gagctcaagc gcgaattcct cgccccggcg attgcgggcg
     9601 aagcggttgg ttgtataggc gtgagcgagc cgggcgccgg cagtgacgtg gcgtccatca
     9661 aaagccatgc gcgcaaggac ggtggcgact atgtgatcag cggccagaag atgtggatca
     9721 ccaacagcct gcaggccgac tggatgtgca tgctggtcaa caccggcgac ggaccgcagc
     9781 acaagaacaa aagcctggtc atggtgccca tgcgcgagaa gggcaagttg cgcaagggca
     9841 ttgaggtcgg caagaagatc aagaaaatcg gcatgaatgc cagcgacacc ggcctgattt
     9901 atttcgacga ggtgcgtgtg ccgcagcgct acctgatagg cgcggaaggc tcgggcttca
     9961 tgtaccagat gatgcagttc caggaagagc ggctctggtg cgcggccagc tgcatccagt
    10021 cgctcaccaa ttgcattgac tggacggtgg agtgggccca cgaacgcaag ctgttcggcg
    10081 ccacgctggc cgaccagcaa tgggtgcagt tcaagctggc cgagatgaaa accgaggtcg
    10141 agagcctgcg cgccatgacc taccgcgcct gcgaactcta tgtgcagggc caggacgtga
    10201 ccgagctggc ctccatggcc aagctcaagg ccgggcggct gaaccgcctg gtgcccgaca
    10261 cctgtctgca gttctggggc ggcatgggtt tcacctggga aaacaaggtg gcccgcatgt
    10321 tccgcgatgg ccggctggcc tcgataggcg gcggtgccga cgaagtcatg atgggcatca
    10381 tcgccaagta catgggcctg tccaaaagca gcaagaaacc cgctgcctga ggatgagcac
    10441 ggccatgagc gacaagctgc agatcacccg ctccggtgag gtcgaacact ggaccctgca
    10501 cgatccggcc acgcgcaacg cactgaccga cgaggtggtg gcggcgctgg cacaagcctg
    10561 cgcacgcgcc gcgcaggaca gggccttgcg ttttgtcgtg ttgcagggga gcggcggtgc
    10621 attttgtgca ggcggcagtc tgggtggctt tgcaaagtcg atagggcagg ccttaccgct
    10681 cggccaaacc gatccgctga ttgccatgaa cgcaggtttt ggccacctgc ttggcaagct
    10741 gtgcgccctg ccgcagatcc tccttgtggc cgcagacggg ccggccatgg gtggcgggct
    10801 ggggctgctg tgttgcggcg acttcgcgct ggccagtccg agcgccgtgt ttgccacgcc
    10861 cgaggtcacg ctgggtgtgg tgcctgcgca gattgcgcct tttgtacaca ggcgtctggg
    10921 tgacaggctg gcacggcagc tgatgctgtc cggagaaaaa ctggtcgctc agcaagccct
    10981 ggcacaaggt cttgtggacg aggttgccga cgatctgccg gccgccgtgg cgcggcgcat
    11041 cagcgcgctg cgccgggcgg ccccgggcgc cgtggcccac accaaggctt tgctttacat
    11101 gctgaatcag gctccagccc atgacctgtc tgcgcaagct gctatcaaat ttgcagcaaa
    11161 cctgcgcagt cccgaggccg ccgaaggcct cgcggccttt gccgaaaaac gcaagcccgg
    11221 gtgggcggca tgagcaccac aaccccgacc atcaacatca gccgcctgct gatcgccaac
    11281 cgcggcgaga tcgcccgccg catcatgcgc accgcgcacc gcatgggcat ggccaccgtg
    11341 gccgtgtatt ccgatgtcga cgcgcaggcc ctgcacgtgc aggaagcggg cagtgcggtg
    11401 gcgctgggcg gcacgctctc agccgattcc tatttgcgca tcgacaagtt gttggctgcc
    11461 gccaaggcgg ccggcgcgga tgcggtgcat cccggctatg gtttcctgag cgaaaacgcg
    11521 gagtttgccc aagctgtgct cgacgccggc ctgacctggg tcgggccacc accggcggcg
    11581 attcgcgcgc tgggcaacaa gagcagtgcc aagaatctgg cgctgcagca aaacgtgccc
    11641 tgcctgagcg gttaccaggg cccggaccag tccgacacct gcttgacggc cgaggcggac
    11701 aaactgggtt acccgctgat ggtgaaggcc gcagcaggag gcggcggccg cggcatgcgc
    11761 ctggtgctgg agccgtccct gctgcaggcg gccctgcaca gcgcgcgctc cgaagccctg
    11821 tcatcttttg gcagcgacga gttgctgctc gaacgcgcgc tcctgcgccc gcgccatgtc
    11881 gaggtgcagg tgtttgccga tgcacatggc cactgcattc acctgggcga gcgtgactgt
    11941 tcggtgcagc ggcgtcacca gaaactgatt gaggagtcgc cgagcccggc agtgaacgct
    12001 gagctgcgtg agcgcatggg tgcgtgtgcg gtggcgctgg cgcaggcggc cggctacgtg
    12061 ggcgccggca cggtggagtt tctgctcgac gagaacggtg attttttcct catggagatg
    12121 aacacccggc tgcaggtgga gcacccggtc accgaggcgg tcaccggcct ggatctggtc
    12181 gagtggcagt tgcgcatcgc gcagggcgag gttttgccgc tgtgccagga cgacgtggtt
    12241 ttccgcggtc acgccatcga ggtgcgcctg tgtgccgagg acgatgattt ttcgccgcat
    12301 gccgggcggg tgcaacactt cagtgagccg gcgcacgcgc ggtttgacca tgcgctgcag
    12361 gctggcggcg aggtcacgcc gttttacgac tccatgctcg gcaagctgat cgtgcatgga
    12421 gccacgcggg cgcaggccat ggacggccag gtggccgcgc tggccggcac gcaggtgctg
    12481 gggctgccga ccaaccgcgc ttttctggcg gcgtgcctgg cgcacccggt gttccgcgcc
    12541 ggccaggcgc tgattccctt tctggccgaa catggtgacg gcatccgcca gaccttgcag
    12601 gaccagcaaa cgcaggtgtt gttgcccgcc gtcctggggc tgcacttcca gggcggcgcc
    12661 ggcgctgcgc agtccttgcc ctgtccgttc ggccaagccc tgcgcgtgct gcacggcgaa
    12721 caggtgctgg ccatcaccgt gacggaaacc ggcgcgggct ggctggaagt ttcgtcaggc
    12781 gcgcaccggc acacggccgc ctgtgtgcgt ggcagcgacg gccggatgcg ggtcacggtg
    12841 gacggcctga gccgcacggt gcaggcctgt cgggtggccg gcgggcgctg gcatgtgcag
    12901 gtcggtgcgg tcgatgtctg gctcaaggac gcgagttttg agcctgcggc cggagtcgcc
    12961 tccagcagcc aggccatgga actccgggcg cccttcaatg gcaaggtgat cgcggtcaag
    13021 gtgagcgctg gcgaatcggt gaaacgcggc gacaccttgc tggtgatcga gtcgatgaaa
    13081 ctcgaacatg cggtgtgtgc gacgcgcgat gcggtggtgg cggccatggc cgtggagccc
    13141 ggccagcaga cggcgcccgg ccaggtcttg ttgaggtttg aagcatgaac cctgactcca
    13201 ccgatctcga catgctggcc gacatcaccg cgtcggtccg gcgtttcgcg cagaacgaca
    13261 tcgcgccgca tgtcagccag tgggatgacg ccggcgagtt tccgcgtgcc ctttaccacc
    13321 aggcggccgg gctgggcctg ctggcgctgg ggtaccccga ggccctgggc ggcagcccgg
    13381 cgtcctggcg gctgcgcaac gcgatgacgc tggcgctgtg ccgtcatggc ggcagcggcg
    13441 gtgtgctggc cagcctgttt tcgcacaaca tcggtttgcc accggtcttg gcccatggct
    13501 cggcggcctt gcagcaggcg gtgattccgc cggtgctgcg cggcgaaaaa attgcagcac
    13561 tggcgattac cgaaccgggc ggcggctcgg atgtggcggc cttgcgcacg acggcgcgcc
    13621 gtgacggcgc cgactatgtg atcgacggcg agaaagtatt catcacctcc ggcatgcgcg
    13681 cggacttcat cacggtagcg gtgcgcaccg gtgagcccgg cagcaaaggg gcaggaggca
    13741 tctcgatgat cctcgtgccc ggcgacagcc caggcctgtc gcgcacccgg ctggaaaaaa
    13801 tgggctggtg gtgctcggac accgcgcagc tgcgttttga cggcgtgcgg gtgccggcca
    13861 gccatttgct gggcgaagaa gggggcgggt tcaggatcat catgggcaac ttcaacggcg
    13921 agcgtttggc catgtcggcc gcggccctgg gttttgccca ggcctgttac gacgaggccc
    13981 tggactgggc gcgccagcgc aagactttcg gcagcacgct ggtcgacaag caggtcattc
    14041 gccacaagct ggtggacatg cagatgcgca tcgcctccac ccaggcctgg gtcgatgcgg
    14101 tggccgaccg tgccgatgcc ggcgaccagg gccccgactg ggtcgcccat gtctgcatgc
    14161 tgaagaacca cgcgacgcag accatgcagt tctgcgccga ccaggcggtg cagatcctgg
    14221 gcggcatggg cttcatgcgc ggcaccaaaa gcgaacgcat ctaccgtgag gtcaaggtca
    14281 tgatgattgg cggcggcgcc gaggagatca tgaaggacct cgctgcacga caattgggcc
    14341 tatgaccaga aacaccacac cgcccgccgg caccgtcgaa ttcgagcccg agttcatcgc
    14401 tgccctgaaa acactttttg aagaaaaaat tgtattcaac caggccctgg gcctgaaggt
    14461 ggtgtcggtt cgtcccgaag gcgttgttgg ccgcatcgac atgcagcctt cgctggtggg
    14521 ccactatgcc tacaaccgca tccatggcgg ggtcgtgagc gcggggctcg atgcgatggc
    14581 ggggctggcc atcatggctg cgatcggtgc acgccacatg gatgaaaccc cggcccagcg
    14641 catgcaccgc ttctcgaagc tgggcaccat tgacctgcgc gtggactacc tgcgtccggc
    14701 catcggcgag ttttttgaac tgcgcgccga ggtcatgcgc ctgggttcac gcgtcgcgtc
    14761 cacccgcatg gagtttctcg gcgcggacgg caagctgctg tccaccggcg ctggcgctta
    14821 catcgtgtca tgaaaaaagc cgcccgaagg cggcttttga cgaaaggctg tccgcttacc
    14881 tgatgtagtc ggacaccact ttccattttc cgtcggtgat ttgcgacagg cgcgaagcat
    14941 cgctgcccag gcgcttggtg ggcgagaagc tggcgggtgc gctgccgaac atgtcgggcg
    15001 gaatgctcat ggtgtccatg accttgatga agctttccgt cgtcaggttg gttccggctt
    15061 tctgtgcggc tttggcaaag gtgtcgatga tgttgtaacc gtagaccgag aaaacggtgg
    15121 ggtcctcatt gaacttggtc ttgtacttgt tggcccagaa acggatgggc tgcgaagctt
    15181 catcgaggta ggggttctgg accgtcatgg tggcatagag gccattcatg gccgggccgc
    15241 ccagcttgtg gatcaggtcg gtgtaagacg cgctggagcc caggaagatc gggttgaagc
    15301 cggttttgcg ggaggtacca atggcgccta tggtttcacg gatgatggtg cccagcacca
    15361 ccatctcgca gccggccgct ttcatcttgg cgacctgtga cgagaaatca gtcgcaccgc
    15421 gcttgtacga ggttttttcc gcaaactcca tcccgatggt tttcaggccg gcttccgcgc
    15481 cccgcaccac ctccaggccg aactcgtcgt cctggtacat ggtgcagacc ttcttgacgc
    15541 ctttttcctt ggccagcttg ggcgtggcgt tgcggacctg gtcatagtag gtagccgcaa
    15601 aggaatactt gagcttgtgg aagggctcgt acatttcgcg cgccgcggtg atcgggaaaa
    15661 aattgatcac gttcttttcg aactggaccg gcatggcggc cagattctgc gccgtgccga
    15721 tgtggccgac catcatgaaa atcttgtcct ggttgaccag tttctgcgcg gccagaacgg
    15781 attttttggg gtcgtaggca tcgtcttcaa caatgatctt gagcttgcgg ccgttgatgc
    15841 ctccctgctc gttgatctcg tcgacccgca gcagcatgcc caggcgcgcc tgcttgccaa
    15901 accccgcgat ggggccggac aggtcctgga tcgaacccag ggtgatctcg gtcttgctca
    15961 cgccctgctg ggctgcggcg tagccggtac tgagcgccag aacggccagt gccagggtgg
    16021 gtttgaattt catggatgtc tcctgtaaaa cgagggtgct tgcagcttgg cattcttaac
    16081 cttcgcccgc gcctgtcaag ctggcgtatt cacttagggg gcacccctgc cattgcggtt
    16141 cgtgcctcaa aaaagaaaaa aggccgcccg aaggcggcct tgtcacatgc gcgaccctgt
    16201 cagggttgtt cctgatccgg gcgccggtgc aggtttagtc cttgaactga tccgaaccaa
    16261 tcttccaggt tccgttcttc tgcagcacgg ggcgcaggcc attgaatgcc acgtattcgg
    16321 acaccacttt ccagcggccc tcctgcaact gggaaagccg cgagaagttg ttgcccaggc
    16381 gcttggtcgg gctgaagctg gcctccgcgg tgccgaacat gtcgcgcggg atcaccatgg
    16441 tgtccatcgc cttgatgaag ctgtcagtgc tgaggtcggt gccggctttc tgggcgccac
    16501 gaatgaaggc atcgacggcg gcgtaaccat aaaccgagaa caccgtcgga tcatcgttga
    16561 acttggtctt gtacttgttg gcccagaagc ggacgggctg ttcagccgcg tccaggtagg
    16621 ggtgttgcga ggtcatggtc gtgaacagcc cgtccatggc cttgccgccc agcttgtgga
    16681 tcaggtcggt gtaggcggcg ctggagccga gaaagagggg attgaagccg gtctttcgcg
    16741 actcgccaat cgtgcctatg gtttcgcgaa tgatggtgcc gagcaccacc atctgacagc
    16801 cagaggcctt catcttcgca acctgcgaag agaaatcggt cgcgccgcgt ttgtaggagg
    16861 ttttttcggc gaacgccatg cctatggttt tgagtccggt ttcggcgccg cgcagcactt
    16921 cctggccgaa atcgtcgtcc tggtaaatga tgcagacact cttgatgttg cggtccttca
    16981 ccagtttggg cgtggcaatg cggatctggt catagtaggt ggcggcaaac gcgtacttga
    17041 ggcggttgaa aggctcgtac atctcgcgcg ccgcggtgat cgggaagaag ttgatgacgt
    17101 tcttctcgaa ctggacgggc atggcggcca gattctgcgc tgtgccgatg tgaccgacca
    17161 tcatgaaaat cttgtcctgg ttgaccagtt tctgcgcggc caggatggcc ttcttggggt
    17221 cgtacccgga gtcttcgacc agcagcttga tcttgcgtcc attgacgccg ccctgctcgt
    17281 tgagttcttc cacgcgtagc gtcattccca tgc
//
LOCUS       DAIDFG010000028        17104 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_28_length_17104_cov_16.509707, whole genome shotgun sequence.
ACCESSION   DAIDFG010000028 DAIDFG010000000
VERSION     DAIDFG010000028.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 17104)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17104)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17104
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28_length_17104_cov_16.509707"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3109 a         5602 c         5392 g         3001 t
ORIGIN      
        1 tgacgctcaa ggtagtcggt acggcgctgg ccggcaaagc ctggcagggc tcggtcggca
       61 ccggcgagtg cgtgaagatc atgaccggcg ccatcatgcc ggccgggctg gacaccgtgg
      121 tgccgcagga atttgtcagc gttcaggcgg acaccgtcac cctgcccgcc caactgctgc
      181 agcccggcga caaccgccgg ctgcgcggcg aagacctcat gcagggtcgg ccggcgctat
      241 caaaaggtga gcttctcacg cccgcccggc ttgggctggt ggcctcttta ggcctgaaaa
      301 gcgtgaccac ccggcgggcg ctgcgcgtcg cctacttttc caccggcgat gaaatcctgt
      361 cgctgggcga ggcgccgcgc gaaggtgcgg tctacgacag caaccgctac acggtgttcg
      421 gcatgctcac gcggctgggc tgcgaggtga tcgacatggg cgtggtgcgc gacgacccgg
      481 ccctgctgga ggcggccttc acgcgtgctg cggcgcaggc cgacgccatc atcacgtccg
      541 gcggtgtcag tgtcggcgag gccgattaca ccaaggccat gatgaaaaag ctcggcgacg
      601 tggccttctg gaaaattgcc atgcggcccg ggcggcccat ggcggtgggc cggatccctc
      661 aagaaaaatc ggcctccagc ccaatgactg cgggcgcaag cagcttccaa aacaatagca
      721 atccgaacgg ggcgattctt tttgggctgc cgggcaaccc ggtcgcggtg atggtgacct
      781 ttctggcttt tgtgcgtccg gccctgctgc agatgatggg taacacggcc ccggacgctc
      841 cgctgctcaa ggcccgcagc ctggagccca tgcgcaagaa gccgggtcgc accgagtacc
      901 agcgcggctg ggtgtccacg accgccgacg gttcactgca ggtcaggacc accggcaacc
      961 agggctcggg cgtgctcagt tccatggcgc aggccaatgg cctgatcgtg ctgcaccatg
     1021 cccagggcag cgtgaatgtg ggcgaagagg tcgatgtcat gatgttcgag ggtgtgctct
     1081 gaagccgtgc acctgaccag cagccgcccg tcctgacggc gcggccagga gacctcatgt
     1141 attccatcat gcccgccctc gtgtccgccc tgtttctggg gtatgggctg tacgtcgtcg
     1201 cggaaaaggg ctttacccgc gtcagcacca gcttcctgat tctctgcatc accaccgtct
     1261 tctggcagac cacctgggcg atattgttcc aggtcgacaa cacgcagtgg gccgctatcc
     1321 tggtcaaggc cggctacctg ctgatccttt tcctgccgac cagcctgtac cagttcctga
     1381 ctgaaatctc ggagcgccag ggcgaacgcc ggctggtctt cacctcgtat gccatcgctg
     1441 ccgtgctggc cgtgctggat ctgggcacca actggatcgt cgacggctac caccactact
     1501 tctttggcta ctatcccaaa gccggcatcc tccaccccct gcatgtcctg cagacggtgc
     1561 tggtggtttc ccggggcctg tacatcacct tccggcaaca gcaagttgca ccgcaaggcc
     1621 agcgcatccg cctgcggctt tgcattgcca gcgtgctgat ctactttttc gccgccatcg
     1681 actacctgtg caactacggc gtcgagttct acccgcctgg cgtcgtattc atcgccatca
     1741 gcctgggact gatcgccgtt gccgtgatga agtacgacct gatgagcccg gtggtagtgg
     1801 ccgccaccgt ggcccatgaa atgcgcacgc cgctggccac catacgtctg caggcagaag
     1861 cgctggcgca attccttccc gaactgagca gggggtacga gctggccgtg tcccacggac
     1921 tgtgcgagtc cgcattcagc cccggggcct cgcagaaaat ctccgacctg tcacacggca
     1981 tccggcacca ggtggaccgc tccaatgccg tgatcgacat gatgctggcc tcggcccgga
     2041 tggagcagat agacaccagc acattcaggc ggcaccgggc cggcgactgc gtgacggagg
     2101 cgctggagac ctaccccttt tccaggggcg agcgcgagcg ggtcagtgtc cacgtcgtgg
     2161 gcgacttcga gttccatggt tccagcccgc tttttgtctt tgtgctgttc aatcttctga
     2221 aaaattcgct gtatgcgctg aaggcggcgg gcaagggctc gatcaccatc accctcaatg
     2281 ccggcacacc tgttcacacc ctgacctttg tcgacaccgg caccggcatc gccgcgtcca
     2341 ccctgccgaa aatattcgac accttcttca ccaccaaaaa atccgcgggt gccggcatcg
     2401 ggctggcctt ctgccggcgt gtcatggctt ctttcggcgg gaaaatgcgc tgcgaatcgg
     2461 tcgaggggca atacaccctg ttcgccatgg agtttccagc cgaactgccg gcaaccgaac
     2521 caggcagcga gcacccggga gcgctcgccg cgtgaaaccg gcgccgccga tcagcttttc
     2581 aggacggcgg tcggcaactc atactccctc accttgcgca ggatgtcctc gggcagccgc
     2641 tggatgcgtt tgtcatggct ggcaaacacc acctgctggt agccggtgga cacgagctgg
     2701 ccgtccacca tgaactggaa aagcaggtaa aacgaacagt gcctgacctc gaaggtattg
     2761 agctggcact ccaccgcctg gaagggaaat gtctcgtgca catagtcctg gtgggcacac
     2821 ttggtgatga atacaccgct ggcctgcagc atgtcggcgg aaatgcagcg gtgaaaccag
     2881 cgctcgcggc aaacaccctg ccactcgaag tagcgcgaaa aataagtgtt ggcataggcg
     2941 ttcgaatcct tgaggtagat gtcgatggtg tggtggaagg ttttcgaagc cgctacctgc
     3001 ggctgcggca gcggcgcggg tttcggttga agctggacgt gtccattggt ttgcttgagc
     3061 aacatggttt ttcctgggtc tgtagtaaga atcggctacg aaagcagccg gcgtgatacc
     3121 gccaccgagg gcggcacacg ctgaatctac agaacagccg gccggtgctg ttgcggcacc
     3181 ggcttatttg gcgatgagtt cgccgtggac gtcggcgggc agctctttcg ccacggcctt
     3241 gttggcgccc ggaatcttgc tgcgggcaaa cagcgcgtac atggtcggca ccacgaacac
     3301 ggtgagcagc gtgcccagcg acatgccacc cacaatcacc cagccgatct ggatgcggct
     3361 ttcggcgccg gcaccggtgg ccagcgccag cggcaaggcg cccagcacca tggcgccggt
     3421 ggtcatcagg atggggcgca ggcgctgcgc ggacgccttg accagcgcct ccagcatgtc
     3481 catgccctgc ccgcgcagct ggttcgtgaa ctcgacaatc aggatgccgt gtttggtgat
     3541 cagcccgacc agcgtgatca gcccgatctg cgaatagacg ttgagcgagc cgcccgacaa
     3601 cttgagcgca atcagcgcac ctatcatcga cagcggcaca gacagcatga tcaccagcgg
     3661 gtcgatgaag ctttcaaact gcgcggccag caccaggaaa atgaagacca gcgccagcac
     3721 aaacacaatg gccaaggcgc cctgggaatt cttgaactcg cgcgaggtgc cgttcaggtc
     3781 ggtgctgtag ccgggcttga gcaccctggc cgaggtcgcg ttcatgaagg tcagcgcctg
     3841 gcccagcgaa taatcggggg ccaggttggc ggtgatggag gcggcccgcc gctggccgaa
     3901 gtgattgagc tcgcgcggcg agaccgcctc gcgcaccgtg accaggctgg acaacggaat
     3961 catcgcgtcg ttgcggccgc gcacataaat gccctcgatg tcttccggcg tgctgcggcc
     4021 gctggcctcg gtctgcacga tcacgtcgta ctgctcggcg tcgcgcttgt accgcgtcac
     4081 gttgcgaccg cccagcatgg tttcaatcgc cttggccacc acttccacgc tgacgcccag
     4141 atcggccgcc ttgtcgcggt taacgtcgat gcgcaactcg ggcttgttca ggcgcaggtc
     4201 cgacaccggc gactggatgc ccgggttttt ggcgacctcc gcgaggatct gctccaccac
     4261 ccggtccagg ttctcgtagc tgtccgaggt ctggatcaca aaattgagcg gccgctcgcg
     4321 aaagccctgg cccagcgagg gcggcgtgat cggaaacgcc gtgacgcccg gcaggctggc
     4381 gaacttgggc tgcagttcgc gtgccatctc cagcgtgctg cgcttgcgtt cgtcccagtc
     4441 caccgtgcgg tagatgacgc tggcctgcga cacggtgggg ttgccaacgc tggcaaaaat
     4501 ccggtcgaac tccttgtagg gctggcccat tttctccagc gcctgcgcat aacggttggt
     4561 gtagtccagc gtggcgccgt cgggcgcatt gacggtggcc aggatggtgc cccggtcttc
     4621 cagcggcgac agctcctgct tcatggtcgg gaagatcagc aaaatggcca gcgcactggc
     4681 cagcatgaca cccaccacga tccagcgcgc ctgcagcacc cacgccttga ccccgccgcc
     4741 ctggccggcc accggctgat aacgccgcgt aagcacccag cgcagcacgc tggcgtagcg
     4801 gtcggagacc gcattgagga agctctccat gccgcggtca aacacattcg gtgccgggtt
     4861 gtgtttgagc agcttggcgc acagcatggg cgtcagggtc agcgccacaa agcccgacac
     4921 gaccaccgcg ccggccagcg ccagcgcaaa ctcgacaaac aaccggccgg tccgccccgg
     4981 ggtgaaggcc agcggcgcgt aaacagccac cagcgtcgcc gtcatggtga ccacggcaaa
     5041 gccgatttcc cgcgcgccct tgatggccgc cgaaaacgga tccagccctt cctcgatgtg
     5101 gcggaagatg ttttccagca tcacgatggc atcgtccacc accagcccga tggccagcac
     5161 cagcgccagc agggtcagcg tgttgatggt gaagctcgcc agcgccatca gggcaaaggt
     5221 gccgatcagg ctcaccggga tggtgatgat gggaatgatg gacgcgcgaa aggtgcgcag
     5281 gaacacaaaa atcaccagcg ccaccaaggc caccgcttcg gcaatggtgg tgtagacgtt
     5341 cctgaccgaa cggtcgatga acaccgagtt gtcgttggcg atgtcgatgt tgacttccgg
     5401 cggcaggtcg gccttgagct gcggaatcat gttgcgcacg ccctgcgaca ggtccagcgg
     5461 gttggccgtg gcttggcgga tcacgccggc cccgatggcc gcgcggccat tgagccgcac
     5521 gccgctgcgt tcgtcggccg caccttcctc gacacgcgcc acatcgcgca ccttgaccgg
     5581 aaagccattg acactgcgaa tcaccacctc gccgaactgc tcgggacgca ccaggccggt
     5641 ctcggacgtc acgctgaact cgcgttgctg cgactcgatg cggccggcgg gaacctccag
     5701 gttgctgcga cgtatggcgt cctcgatgtc ctgcgtcgtg aggcggtagg cggccatctt
     5761 ccggggatcg agccagacac gcatcgcgta tttgcgttca ccgaagatgc gcacatcggc
     5821 cacgccggtc acggtttgca ggcgtggctt gacgatgcgg ttgacgaggt cgttgatctg
     5881 cagccgggtc aggctgtcgc tggtgaaggc cagccagatc acgggaaacg catcggcctc
     5941 gaccttggca atcaccggct cttcaatcga ctgcggcaac ttgttgcgca cgcgcgaggt
     6001 gcggtcgcgc acctcggcgg ccgcggcatc ggcgtctttc tcgagacgga aacgcacgga
     6061 aatctggctc tggtcggcgc gactgatcga ggtcagcaca tccaccgcgt cgatgccggc
     6121 aatcgagtcc tcgagcggct tggtcacctg cgattcgatc acctcggccg aggcaccggg
     6181 gtaacgcaca ctaaccgtca ccaccggctc atcgatcttc gggtactcgc gcaccgccag
     6241 gcgctggaaa ctgaccgcgc cgaccagcac gatcagcagg gacagcacgg tggcaaacac
     6301 cgggcggcgt atcgagactt cagccagttg catcagaggg gtccagtcag gggatggctt
     6361 ggtgctcagg ccggcttgcg tgtcgcccgg gccctggctt caggcatgac ctcggtcgga
     6421 gctggcgcgg agctgccgcg tgtcgctccg gacaccgcag gcgcgggacc cgcctcacgc
     6481 atgcccacca ggcagggatt ggtacccccc agggcttttt ccgtcagcac gcgcgaagcc
     6541 tcaggcgccg ccgccgccgc caccggcgca gcggcagacg cacccttgga cgaccgtgcg
     6601 gcggcgggcg ggctcaggtc caccacccgc acggtcatgc cgtcacgctg aaggcgttgc
     6661 tggccggtgg tgaccaccat gtcgccgggc tccagcccac tggtgatttc cgcctggcct
     6721 ggccgccgga taccgacctg caccgccacc cgccgcgcga cccaggtctg ggcttccggt
     6781 ccgggctgca gcttgatgac aaactgcctg tcgccctggg gcaccagcgc ttcttccgga
     6841 atgaccctgg cgttgtcccg ctcgccgaac accgcgttga cacgggcaaa catgccgggg
     6901 cgcaactgca gctgacggtt gtcaatgcag gcccgcaccg ccaccgagcg gccgttggcg
     6961 tcgatcagcg gatcaatcgc ctgcaccacc gctgtgtatg tccggcccgg cagcgcgtcc
     7021 atgtccacca gggcggtctg gccgcgcttg agcttgttct gaaagcgctc gggcagacgg
     7081 aaatcgacaa acaccgcgtc gatgtcttca atattgacaa tgtcggcccc atccttgagg
     7141 tagtccccgg ggttgaccag gcggatgccg gcaatgccgt cgaagggcgc gacgattttc
     7201 agcctggccg ccgtggccct cgccagcgac agcctggcct gcgccacctg cagattggcg
     7261 gcgctttcat cgagggagcg ctggctgaca aagttctgcg cgaccagttc ctggttgcgc
     7321 ttctggttgg cctgggcgat cgacaactcg gccaggcttt gctgcacctg cgccaacggc
     7381 agctggtcgt caagctgcac cagcacctgc cccttgcgca cgcgctggcc gtcggtgaaa
     7441 ttgagctggg tgatgcggcc gctgacctcg gggcgcagca ccacgctgcg tcgtgaacgc
     7501 aggctgccga cagcctgggc gtcatccacg agtttcacaa ctttcaccct ggcggcttca
     7561 actgtcggtc gcgcaccggc tgccggctgc gatgccctcg tcgacgcagc cctggccggt
     7621 gccttgttct ggtaccacca tgcggcaccg gacaggccgg caatgccgag caccgcgacg
     7681 acagaataaa ttgcttttga tgccatgggg aacggttgat gaggggtcaa aaatctgaca
     7741 atggatcaat gtaacagcag cttgcgtcac ccgggtcacc ggggtgaaat cccgagcaaa
     7801 atcggggccg gctttacatt gacaccacca cccgccgccg gcaggatcag cccctggaca
     7861 gggccagaac cacacctttg ttggccaggg catcggcgcg ttcgttgccg gggtcgccgg
     7921 catggccctt gacccagcgc cactcgatgg tgtggccact gctggtgatc agcttatcga
     7981 gtttttgcca gagatcgatg tttttcacag gctgtttggc agcagtgcgc cagccgcgtg
     8041 ctttccagcc gctgatccat tcggtgatgc ctttgcgcac gtattcgctg tccaggtaca
     8101 gcgtgacgcg gcagggacgc ttgagcgcgg ccagcgcttc gatgaccgcc atcatctcca
     8161 tgcggttgtt ggtcgtgccc agttcgccgc caaacagttc cttgaccgtg tctccgctgg
     8221 tcagcatcgc gccccagccg ccggggccag ggttgccctt gcaggcgccg tcggtataaa
     8281 tcaccacatg ttgtgccgtg gtttgcggaa gatcagtcat gcgttgcagg aatccatggt
     8341 tgttggtctg ggaaaagaaa aagtggtcaa aaggattccg gtcggcgtac ctggttggca
     8401 atgaccgccg gggcctgcgc ggcggccttc tgcgttttcc agttgggttc gagcaggcgc
     8461 atgccacgca cgcgcttgac ggccaccaga aaatacatcg cgccgaaaat aggccaccag
     8521 cgttcgccgg ccttgtccat ccaggcaaaa cgctccagcg atgcaagccg gctcacggcg
     8581 ggccggtagc agccgaaccg gccggactcg acttcaaagc tgagcagccg cagccagtcg
     8641 cgcagccgcc agtagccgat gaactcaccg gcttcgggca aaaaaagttc gccgtaaccg
     8701 aggcgccggt aaaaatgcgc gcgccgctgg cgcaagcccc acaggctggc cgggttcaga
     8761 ccgctgatca ccacgcggcc ctcgggcacc agcacacgtt cgacttcgcg caatgtggca
     8821 tgggggtcgc tgctgagttc cagtgtgtgc ggcagcagca ccaggtccag gctgttctcg
     8881 ggaaagggca aggcggccga atgggtcatc agggccacac gcggggaact tgccgcaggt
     8941 gcaggggtcg gactgccgtc accgccttcg gttggcccgg cggtaagccc cgactcggtg
     9001 ccctgcagtg ccagccactt gtgcggcatg cggttgctgc gcagcgcgtc gagttcgggc
     9061 aggcccagtt gcagcgcatg gtagccaaac atgtcggcca cggcttcgtc gaatcgctca
     9121 cgctcccacg ccagcaaata ctcgccgggc ggggttttga accagtttgt gaaacctata
     9181 atttgagagc ccatcgagac tgctgagact gctgcatgta cctgattccc atccctgctt
     9241 ttgacgacaa ctacctctgg ctgctgcacg acggccggcg cgccgtggtg gtcgatccgg
     9301 gcgacgcgca gcccgtgctt cgcatactgc aggacaggca actgcaatta gagtcgattt
     9361 tagtcacgca ccaccacccc gatcacaccg gcggcgtcga tgtgctgcgc gaagccaccg
     9421 gtgcgaaggt gttcggcccg gcccgcgagc gcatcccctc acctttcacc cccctccagg
     9481 atggcgacac ggtgccggca ctggaccttg atttccaggt gatcgacgtc ccgggccaca
     9541 ccgcgggcca tatcgcgttt tacagtccca gtgtggacgg gcggcccctg ctgttttgcg
     9601 gtgacacgct gttttccggc ggctgcggac gcctgtttga gggcacgccg gcgcagatgc
     9661 tggcatcgct ggaccgtctc gccgccctcc ccggcgacac ccgcgtctgc tgcacgcatg
     9721 aatacacgat gggcaatctg cgttttgcgc tcgcggtaga acctgacaac cgcgagctga
     9781 ttggttacca tgcacgctgt gctgctttaa gagacgatgg ggaaccgacc ttgcccacct
     9841 cgatagccca ggagttgctg atcaacccct ttttgcgtac ccgacaggcc actgtcatgg
     9901 ccgccgcccg gcgctttgat ccggcggcgc atgacgacaa caccatattt gccgccatca
     9961 ggcagtggaa gaaccaattc aaatgaccga gctgcagcca gcatttttca ccctctcccc
    10021 cgccttcccg gctctccctg accgttttgc acggctgctc tccccgctca gaaccttcgc
    10081 cctgtccctc gccgtggtcg tgctcgccgg ctgtgccagc agcaacagca agctcggcat
    10141 gcccggcgat ccggtcctga gcacctcccc atcttcgaca cagccggtct accccaacgg
    10201 ccccctgtcg cccatccatt cggcgcaaac cccctccagc gccgtggcca gcctgcagcc
    10261 gcctgccgat ttgtgggaac gcattcgccg cggcttcgcc atgcccgacc tccagggcga
    10321 gctggtgacg gaccgggagc agtggtacgc cagccggccg gactacatcc agcgcatgac
    10381 ggagcgctcg agcaaatacc tgttccacat cgttgaagag cttgagcgcc gcaacatgcc
    10441 caccgagctc gcgctgctgc cctatatcga aagcgccttc aatccgcagg ccgtctccag
    10501 cgccaaggcc gccggcatgt ggcagttcat gccggccacc ggcaagtact tcgacctgaa
    10561 acagaacgcc ttccgcgacg accgccgtga tgtactcgct tcgacgcgcg ccgcgctgga
    10621 ctacctgcag aaactccacg gcatgtttgg cgactggcac ctcgcgctgg ccgcctacaa
    10681 ctggggcgaa ggcagcgtgg gccgcgccat cgccagaaac aggaaggatg gactgggcac
    10741 cacctacatg gacctgcgca tgccggacga gacgcgcctg tacgtgccca agctgcaggc
    10801 ggtcaagaac atcgtggcca acccgcagac tttcggcgcc gagctgcccc tgattgaaaa
    10861 ccatccctac ttccagcagg tccagatcac ccgggacatt gatgtggcgc tggccgccaa
    10921 actggccgac gtggaaatca gcgacttcaa ggccctcaac ccttcggccc atcgccccgt
    10981 gattctggcg gccggcatgc cccacatcct gctgccctgg gacaatgcca agattttcca
    11041 gcgcaacttc gaggcctaca cccaggggca atacgccagc tggacggcct ggaccgctcc
    11101 cgccaccctg agcgtcagcg acgccgccag acgcgtcggc atgagcgaga ccgacctgcg
    11161 cggtgtcaac aacattccac cacgcatgct gatcaaggca ggctcgaccc tgctggtgcc
    11221 acgcagcgca aaaatggaca acgacgtgac cagccatgtc gccgacaacg cccagatgac
    11281 gctcgcgcct gaaatcgtca cgcgccgcag caccgtcaag gcgcgcaagg gcgaaaccgt
    11341 ggccagcatc gccaggcgct acggtctcag tgcggacgcc gttgcggagt ggaacaaggt
    11401 cggtacttcc gcggctttca agctcgggca ccaggtggtg ctgttcctgc cggtcaaggc
    11461 cgccagagcg gccaggccca gggcgggcaa agtcagggtc caggcggtca ggcgtgtgcc
    11521 gggcaaaacg gtgatcaaga tcaaaaagcg ctagcgcagt agatatctcg ggaaatggcg
    11581 ccatggtcca cctccagcct gtgtcagcag cgatcggcta agtagtccat cggatcacac
    11641 acgccatggc cgtcagcacc ccaggaaggc ccgtcaagcc cagcgcggtc taccggtatc
    11701 ttttcgccat cggactcctg gccgttgcca tcggcgtcac cctgctggtg atgtccatga
    11761 aggagcgggc ctggctgcct gcgggtgctg cggccctgct gctcggggtc gttctggtgg
    11821 gcacttacct gaccgaccgg ctccaggccg aagcttttgt cgaccccctg gccttcgagc
    11881 caccctggag caagctggca aggggtgcgg ggcctccacg gctgcccgct cccggcaagc
    11941 ttgccgtcgc ccccggcaca gcctttgccc tcgacaagct gatcgacttc cccgccgcca
    12001 cgactgccag cagacccgcc cggacagccc agccagcccc gccagctcag ccagcccgca
    12061 gcgtaccacc gccagcccct gtggcgcagc cctcgtggac gccagccctg tttgaccaga
    12121 tgtcggcgca gcagtttgac gcggtgtgtg aagcgctgtt tgcgcagggc ggctttgaga
    12181 cccgatgcca gtcgcacggc gcagccggtg gcgtgacgct atggctttac tcgcggcatg
    12241 cacaacaggg caagaacagc ccggcggcag tggcctggtg caagcagtgg tcgggccagc
    12301 cggtgggcgt gcgcgagttg caccccctgc tcgacttgat gcgttcccgc gaactcaagc
    12361 gcggcacctg tgcgacgtcc tccatgtgca gcgagaatgc gcgcaagttt gcgaagtaca
    12421 acggcatcaa cctgctggac cgcaccgggc tgctggcgct gattgccggg cgtacccccg
    12481 tccagcaaca ggcactcttg gcgctggcgc tggataagcg ctgaagagcc gtgccccgca
    12541 gcgcggcctg ctcccttctg aaatcctaga tcttgagtga gccggtcccg aacaggccga
    12601 gcaggccaat gatgatcagg taaatcgcaa cgatgaagtt gagcaggcgc ggcatgatca
    12661 ggatcaggat gcctgcaatc agggagacga cagggccgat gctgagatgg agattcatgg
    12721 ggtttccttg cggggagtga atggatgccg ccagtcctcg gggtacggcg gcatggcagg
    12781 caccaatgta gcgaccggcc tggcgcgtgt atgtaggacg aaacgaccag atggaagatc
    12841 agcaccggtt ggtcggcgca tcggtggggc aaagcagcat cagagcttct ccgtctcccc
    12901 gctgcgcggc tgccacttca tgagcttgcg ttcaatgcgg ccgacgatgc cgtcgagcac
    12961 cagcgcaaac gccgtcagca ccacgatccc ggcaaacacg gtgttgacgt cgaaggtgcc
    13021 ttcagcctgc aggatcaggt aaccgacgcc gcgcgccgac cccaggtact cgcccaccac
    13081 ggcgccgaca aaggccaggc cgaccgaggt gtgcaggctg gaaaacaccc agctggtggc
    13141 gctgggcagg tagacggtgc gcagcaactg cctttggttc gcacccagca tcctcgcatt
    13201 ggccagcacc accgggctga cctccttgac gccctggtag acattgaaaa acacgatgaa
    13261 gaacaccagc gtgaccgcca acgccacctt gctccagatg cccaggccga accacatcgc
    13321 aaaaatcggc gcgaggatca cgcgcggcat cgagttggca gccttgatgt agggatccat
    13381 gatcgcgctg gccatgggcg ccagtgccag ccacaggccg caggccaggc ccagcacggt
    13441 gcctatgccg aaggcgagta cggtttcgag cagcgtcgtg cccaggtgca ggtaaatgtc
    13501 ggcgttgccc ggcaggccat cgggaaacag caagctgggc ccgaagccga aaggcatgaa
    13561 ccaggaccag acacgccccg ccaccttgac cggctcgccg aggaaaaacg ccagttgctg
    13621 atcgcgcgag gcgagttgcc acaggcccag gaaagccgcc agcagcccga cttgccagta
    13681 gcggagattt ctgtcggaag gtttgaggga attccacatg gcggtcatgc cgcttttttg
    13741 agttgctgcg cgtagccctt gagcacttca tcgcgcaaca cgtcccagat ctgcgtgtgc
    13801 agttcgatga aacgcggctg ggtgcgcacc tcggcgacgt tgcgcgggcg cggcaggtca
    13861 atcgcgaact cgcccatggg aaccgtggcc gggccggccg acagcaccac cacgcggtcg
    13921 ctcatggcaa tggcttcgtc gaggtcgtgc gtgatgaaca gcaccgcctt ctttttggct
    13981 gcccacaagt ccagcacctc gttttccatg agctggcgcg tctggatgtc gagcgccgaa
    14041 aaaggctcgt ccatcaggat gatgtccggg tccagcgcca gcacctgggc cagtgcggtg
    14101 cgcttgcgca tgccgccgga gagctgatgc gggtaacggt cgccgaagcc gctcaggccg
    14161 acgcgctcca gccaggcctg cgcctgcgca cgcgcctcgg catcagacac gccgcggtac
    14221 tgcagcccaa ccatgacgtt gtcgaccgca ctgcgccagg gcatcagcgc ctcggtctgg
    14281 aacatgtagc cggcgcgggt gttgatgccg ttcagcggct ggccgaatac cttcaccgtg
    14341 ccggaggtcg gcggcaacag gcctgcgccg atgttgagca aggtggactt gccgcagccg
    14401 gtcgggccga ccacggaaac aaactcgcca gcgcgtatgc gcagcgtggt ttcaccgaca
    14461 gcggtgtagg cctggcccgc atcgtcccgc gagcggaagg tacaggtgac atccagcagt
    14521 tcaagcgcgt agtcagaggc aggatcgagg gacacagact gaggcattgt cgcgtgaatt
    14581 tttaccagcc ggggccgcgg aaccggcttt gccgggccgc aggcgcagcg gccccttttt
    14641 cagtgcaagg ggcagagagc tgccgcaggc tcgaagcgag cgaacgtggg ggccacattc
    14701 atgctttgaa cttgtctttc gcgcgccggg caaactcgtt ggtgtaggtc ttggccagga
    14761 caatcctgtc ggccttgatg ctggggtcaa aactggccag ggccttgagc gccgtcttcg
    14821 caccttcttc aggaatcagg ccgtccagcg cgatcgcgtc acgcaccttg ttgaaggatg
    14881 cgaggtacag gccgcgatcc cccagcaggt agttgtcggg cacggtcttg ataatgtcgc
    14941 ccgggccggc ggtttgcagc catttcaggc catgcacgat ggcattggcc agcgcctggc
    15001 aggtgttggg atttttctgg atgaagtcca tgggcgcgta caggcaggcc gccggcatgg
    15061 ggccgccaaa cacgtcgatc gtgcctttga gcgtgcgtgt gtccgagatg atcttcacct
    15121 cgcctttttg ctccagcatg gtcatcaccg ggtcggtgtt gctcatggca tcgatctggc
    15181 ccgagcgcat ggccgccagc gcaccggccg ccgtgccgac gccgatgaaa ctcacgtccc
    15241 tcggcttgag cccggcgcgc gacagcacca ggttggccat catgttggtg gacgagcctg
    15301 gtgcggacac gccgatcttc ttgccacgca ggtctgccac cgttttgtag ttgggcatgt
    15361 ttttggtcga cacgccaaag gcaatctgcg gcgcccggcc ctgcagcacg aaggcctgga
    15421 acatctggtt cttggactgc aggttgatgg tgtgttcgta agcacccgac accacatcgg
    15481 ccgagccacc cactgccgcc tgcagggccc gtgcgccgcc ggcaaagtcg ctgatctcga
    15541 cgtccaggcc ttcggccttg aagtagccga gttgctcggt gatggtcagc ggcagataat
    15601 aaaacgccgc cttgccgcca accgccagcg caatttttgt tttctccagc ttgccctgcg
    15661 cgcgcaaggc cggaaaggcc agcgacgcgg caccgagtgc tgccgcactg gcgaaagttc
    15721 tgcggttgat ggtgaggtgc ttcatggggc gggttctctc tctgattttt ttatggactc
    15781 aaaagagaat agccagcccg aaaaaaaccc gcatcggtat catcccgtgc gggtttgtac
    15841 ggaacgggca cccgccctgg ttaccagacg ttaccggtag cccagaaaaa gaatgcccac
    15901 attcaccagc agcgccagga accagatcac gctgcggctt ttggccatgt cggccacgta
    15961 catcatgacg taggcgatac gcagaacgac aaacacgaac gcaaacacgt cgagccgcac
    16021 ctgcgaggcc tgcaactggt gcgcaatgat cacggcggca aagaagaagg gcaaggcttc
    16081 gaaggtgttg gcctgcgcgg cattggctcg cgcgcgccag tcggtttgcc gcgccagcca
    16141 ggcgcgcggg ttgtggttgt cgaagccgcc tctgttcgcc ggggtcgtca tcgtgcccca
    16201 cttggccagc gccgcgcagg cgatcggcag cagcgcagcg gccagcaggc accaataggc
    16261 cacggtgaaa cgagcatagg tcatggtgaa gtgcctttct ggcttgcagt catgaatggg
    16321 cgggcgcggt cagctataaa aacagtaacc atggacatgg gcgcgcaacc gcgcattaac
    16381 ggcgatccac cagcgcatgg gcaatcgtgc cgaggtccac gtactcgagc tcactgccca
    16441 caggcacgcc ccgcgccagg cgggtgacct gcacgccgcg gcttttgagc gcctgcgcga
    16501 tcacatgcgc ggtggcctcg ccttcggcgg tgaagttggt ggccaggatg acttcctgca
    16561 cctcgcgctc cgcggcagcg gacggatcgc cctgggcgtc cctgggcaac acccgatcga
    16621 acagcttttg caggccgatg tcatgcgggc cgatgccgtc gagcgggctg agcttaccca
    16681 tcagcacgaa atacaggccg cggtaggcca gcgtgcgctc cagcgccgcc tggtcggccg
    16741 gggtttccac cacgcagagc ttgctgcggt cgcgcgctgg attggcgcag gtgctgcaga
    16801 tgtcgagctc ggtcagcgtg ttgcacagcg cgcagtgttt gacgctgtgc accgcatgct
    16861 ccagcgcgcg ggcgatctgc agggcgccgg gcttgtcatg ctgcagcagg tgaaacgcca
    16921 tgcgcgcggc cgacttcgca cccacacccg gcaggcggcg cagcgcctgg gtcaggcctt
    16981 caagggcatt tgaatcagcc atggtggagg gcaaccgcgg cgtatgcagg cctggacacc
    17041 agaccagccg gggccgcgga accggctctg ccgggccgca ggcgcagcgg ccccctcggg
    17101 gggc
//
LOCUS       DAIDFG010000029        16732 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_29_length_16732_cov_17.218025, whole genome shotgun sequence.
ACCESSION   DAIDFG010000029 DAIDFG010000000
VERSION     DAIDFG010000029.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 16732)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16732)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16732
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29_length_16732_cov_17.218025"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2822 a         5199 c         5483 g         3228 t
ORIGIN      
        1 gcgcccgagt tcaaggtgtt caccaacatc cacagcacgg tggtcgaccc gaaaaacttc
       61 gatgaaaaga gttttgtcga ctttcacggc gacagctgca tcatcccgcc caacagcttc
      121 gcgctggccc gcacgctgga gtactttcgc atcccgcgca acgtgctgac catctgcctg
      181 ggcaaaagca cctacgcgcg ctgcggcatc atcgtcaacg tcacgccctt cgagcccgaa
      241 tgggagggtt atgtgacgct ggagttctcc aacaccacgc cgctacccgc gaggatctac
      301 gcgggcgaag gctgcgcgca agtgttgttt ttcgagagcg atcccgacga cgtctgcgaa
      361 gtcagctaca aggaccgcgg cgggaagtac cagggccagg tcggggtgac cctgcccaag
      421 gcctagttct ataccctccg acgtggatgt gggtggtgat gaccgcgccg gtggttgtcc
      481 aacaggaagc gcttgcatca atgaaaaaaa gctgacgttg tcagcttttt ttgtctggtg
      541 gcgcgttatc gcctgaccag ctgcaggcta tttgaccagc agcaccggaa tgtcgctgtc
      601 ggtgaccacg cgctgggcca cgctgcccat gacggcgcgg ccgagaatgc cgtggccgtg
      661 ggtgcccatg acaatcatgt gcgccttttc gcgcttggcg gcgcgcgcca cttccgctgc
      721 gggtgatccg gcgacccaga tcgtgcggta ttgcagcttg tggcgcttga ggaaacgctc
      781 gatggggtcc agcactttct tggcttcgtc ctcatagtag gcgttgacgg tgctcgcacc
      841 caccatgccc ttgacgcgcg gaggaacggg cggctggacg ttgagcacca ccacttcatc
      901 ctcggggccg gccaggcttt cgtgcgtgac gagaaaagcc agggcctttt tggtgtattt
      961 gctgccgtca gcggcgaaca ggactttcat tatttctcct tgtgctggat aaaaaccggt
     1021 tttgaaaaac cagtttagcg caacaccagc acgggaacac tgctgtgtgt cagcacatgc
     1081 tgggtttcgc tgcccagcag gacgcgtttg atgcccttgc ggccatgcga ggccatgacg
     1141 atgaggtcgc atttgtgttt tttcgcggcc gagatgatgg actcggcaac caggtcggaa
     1201 cgcgcgacga cagccttggc cttgaggcct tcggcctgtg cctgcagttg caccgcatcc
     1261 accacagcct ggcctttgtc ggcccattgc ttttcggtgc gggccacgtc ctgcgccgaa
     1321 atcgtgatgc cgccttcgaa atagctgacc gggtaacgcg gcaccacata aagcgcgacc
     1381 agttcggctc cgacgacgcc ggccagatca atggcgctgc gcacggcttt tttcgagagg
     1441 gtggagccat cggtggcaac gaggatgcgt ttatacatgg cggggtctcc tgagggttga
     1501 tgcgaggttt gcagcttagg ctggcgaccg tccggtgcgc ttgatccatg tcaagcgggt
     1561 gtcaccgcga cagggtaggc ttgccccatg cacactgcca ccctcgattc cggcgccgcg
     1621 caacaggcct tgcgctggtc tgctctggac gagcgcgccg aatgggaggg cttcagccgg
     1681 ccgctgcctg ggcgcacgga cctgtgggag tcctacctcg cgatcgaggg catgcattgc
     1741 gcggcctgct cgctgacggt ggaggaggtg ctgcaaaagc ttcccggcgt ggacaacgtg
     1801 caggtcaacg gcgccagcgc ggtggcccgt gtggcctggg cgcccgacca gggccggccc
     1861 tccgcctggc tggccgcgct gcagcgggcc ggttatggtg cgctgcccgc cggcgacatg
     1921 ctggctgcgg cgccgcgtat ccaggcccag cgcatgatgc tctggcgctg gctggtggcg
     1981 ggcttttgca tgatgcaggt catgatgtac gcctttccgg cctacatcgc cgagccgggc
     2041 gacatctcat ccgaagccca gagcctgctg cgctgggcgt cctgggtgct gaccctgccg
     2101 gtcgtgctgt tttcctgctg gccgtttttc tcttcagcgc tgcgtgatct gcgcaaccgg
     2161 cgtgtcggca tggacgtgcc ggtggcactg ggcattctca ttgcgttcgg tgccagctcg
     2221 gccgccacct tcgatccggc ttcgcgctgg ggcgcagagg tctggtacga ctcggtcacc
     2281 atgtttgtgt tcttcctgct ctccggacgc ctgctggagc agcgcctgcg tgaccgaacc
     2341 gccggctctc tcgaggcgct gatgcgcagc ctgcccgacg gcgtggagcg gcagggggcg
     2401 gacggcactt tcgagcgtgt ggccgtgcgc cgccttgtac cgggcgacct gatccgcttg
     2461 ctgcccggtg acgtgatccc ggccgacggc acggtgacgg ccggcgagag ccaggtcgat
     2521 gaagccctgc tcaccggcga gtccacgccg ctgcaacgcc gcgttggcag cgcggtgatt
     2581 gccggcagcc acaacctgaa cggcatgctg ctggtgcggg tggaacgcgc aggcagcgag
     2641 acccgttatg ccgccatggt ggcgctaatg gagcaggcct cggtgcagaa gccgcgcctg
     2701 gcgcgaatcg ccgaccgcat cgccgggcct tttgtgctgc tggttttgct ggccagcgct
     2761 ggtgcggcga tctggtggtg gccatccgac ccggtgcatg cgatcagcgt ggccgtggcg
     2821 gtgctgattg tgacctgtcc ctgcgcgctc tcgctcgcca cgccggcggc gacgctggct
     2881 gctgccggcg ccctggcgcg gcgcggtgtg ctggtgcgcc ggctggaggc gctggagagc
     2941 tgcgccatgg tggacacggt ggtgttcgac aagaccggca cgctgactga agaccgcatg
     3001 gcggtgaccg tcagccggac tcgcgagggc acccctgccg tgcaggccct gtcgctggcg
     3061 gcggcgctgg cgcagcattc cctgcaccca ctgtcgcgtg ccattgtcca ggcggcgggg
     3121 atcgtgccag ggcctgctgt ggatgtcacc gaagtttccg gtcagggttt gcagggccat
     3181 gccgctgccg gcgctggcgg gaagctgtac gccatgcgcc tcgggtcggc cgcattttgc
     3241 ggggcaccgg ccgatgtcca ggccgcgggc ggcccgcagg tgcatttgtc ggatgaacag
     3301 ggctggcttg ccagttttga gatggatgag gccctgcggc cagacgccag gccggcggtg
     3361 ggagcgctgc acgctctggg cctgcaggtg caactgcttt ccggcgacct gatggccgct
     3421 gtcacgcgcc tggctgcgcg cgcgggcatc gacaaggcgt acggacagcg tacgccggaa
     3481 ggcaagctga cccatgtgcg cgagttgcag cagcagggcc ggcgcgttgc catggttggc
     3541 gacggcatga acgacgggcc ggtgctggcc tgtgccgatg tgtcggtcgc catgggccag
     3601 gccgtcccgc tggcgcaggc ccaatccgac ttcatcgtgc tgggtggcca gctggctggg
     3661 gtgtccgcat tgctgcagca tgcgcgccgt acccgcgtca tcgtgcgcca gaacctgttg
     3721 tgggcggccc tctacaacgc cgtgtgtgtg cctctggcca ttctcggctt catgccgccc
     3781 tggctggccg gactgggcat ggccgccagt tcgctgctgg ttgtactcaa ttcagcccgg
     3841 ctggcccgta tgccgggata atagaattac tccatggaca ttctttttct cttgattccc
     3901 ctgtcggtcg gactggcgct gctgattatt gttgcgctgg cctgggccgt ctggcgcggc
     3961 cagtttgact ccgtggaagc cgaaggtgag cgcattcttc gatcggattg acttgcatca
     4021 agttccgccg gcatgccaaa agagacacta cttacatctc gactataggt gactcatgaa
     4081 cgaaccacaa gctcgcactg cgtactacca cgacacggtg gtgcggcagt tttccctgat
     4141 ggccgtggtc tggggcgtcg tcggcatgct ggtcggcgtc atcatcgcct cccagcttgc
     4201 ctggcccgaa cttaacttcg gtattccttg gctcagctat ggccgcctca ggccgctgca
     4261 taccaatgcg gtgatttttg cgttcggtgg ttgcggcctg ttcgccacca gctattacgt
     4321 ggtgcagcgc acctgcagcg tgccgctgtt cgcccccaaa ctggccgcgt tcaccttctg
     4381 gggatggcaa cttgtcattg tggcggcggc gatcagcctg cctttgggtt acacctcagg
     4441 caaggagtac gccgaactgg aatggcccat cgacatcctg atcacgctgg tgtgggtgtc
     4501 ctacgccatc gtcttcttcg gcaccattgg catccgcaag gtgcgccaca tctatgtggc
     4561 caactggttt tttggtgcct tcattctggc catcgccctg ctgcatgtgg tcaacagtgc
     4621 cgaggtgcct tatagctgga tgaagtccta ctccgcctac gccggggtgc aggatgcaat
     4681 gatccagtgg tggtacggtc acaacgcggt gggcttcttt ttgaccaccg gcttcctggg
     4741 aatgatgtac tacttcattc ccaagcaggc cgagcggccg gtctacagct accgcctgtc
     4801 catcgtccac ttctgggcgc tgattttcac ctacatgtgg gccggtccgc accacctgca
     4861 ctacacggcg ttgccggact ggacccagtc ggtcggcatg gtgttctcgc tgatcctgct
     4921 ggcgcccagc tggggcggca tgatcaacgg catcatgacc ctgtcgggtg cctggcacaa
     4981 gctgcgcgac gacccgatcc tgcgcttcat gatcgtgtcg ctgtctttct acggcatgag
     5041 caccttcgaa gggcccatga tgtccatcaa gaccgtcaat gccctgagcc attacacgga
     5101 ctggaccgtc ggccacgtgc actcgggcgc tctgggttgg gtcgggctga tcacgatggg
     5161 ctccttgtac tacctgattc cgcgcatgtt cgggcaaaag aagatgtact cggtcaaggc
     5221 gatcgaggtg catttctgga ctgccaccat tggtatcgtg ttttatattg ccgcgatgtg
     5281 gatcgccggt gtgatgcagg gtctgatgtg gcgcgccctc aatgccgacg gcacgctcac
     5341 ctacaccttc gtcgaatcgg tcaaggcgac ctatcccttc tacgtcgtgc gcgtgctcgg
     5401 cggcctgctt tacctgttcg gcatgacggt gatggcctgg aacacctgga tgaccgtggc
     5461 cagtggccgt tccgtcaccg tggccgtgcc agcgccgcaa gccgcctgag gaaacaccta
     5521 tgagcgataa caaaaatacc accgccggct tctcgcacga gaaagtcgaa accaacaact
     5581 tcctgatgat cgtgctgatc ctgctggttg tcgccgtcgg cggcctggtc gagatcgtgc
     5641 cgctgttctt ccagaagtcg accaccgagc ctgtcaccgg tgtcaagccc tacaccacgc
     5701 tgcaactggc cgggcgcgat gtctacatcc gcgagggttg ctacaactgc cactcgcaga
     5761 tgatccggcc gttccgtgcc gagacgctgc gctacggcca ttattcggtg gccggcgaat
     5821 tcgtctacga ccaccccttc cagtggggca gcaagcgtac cgggcctgac ttgcatcgcg
     5881 tgggcgccaa gtacagcgac gaatggcacc gcatccacct gaacaatccg cgcgatctgg
     5941 ttccagaatc caacatgccc gcctatccct ggctcgagaa gaacctggtc gatgccgccg
     6001 acatggcgcc gcgcatgcgg gcactgcgga tggtgggcgt gccctacacc gatgacgaaa
     6061 tcgccaagtc cgccgaggac gtcaagggca aaaccgagat ggacgccttg gtcgcctatc
     6121 tgcaaggcat gggcctggcc ctgaccaata acttcgccgg caagaccgtt gggggtagcg
     6181 cggccaccgg ggcacccggc gcatcggccc cgccggctgc acctgcagca cccgctgcca
     6241 actgacagga gccacgccaa tggacatcac caccttgcgt attctggcca cgctggccag
     6301 cttcgccacc tttctgggca ttgcctggtg ggcctacgcc cgccacaacc gtgaccgctt
     6361 cgatgaagct gcgcgcatcc cgtttgagca ggattgatca tgagcgattt catcggtaat
     6421 ttctggtcgg tctatgtaac cgtcttcacc ctggtcggta tccttgcctg cctgctgctg
     6481 ctttggatca cagcgcgcaa gaaggtcacc cccagcgccg acaacaccac gggccacgtc
     6541 tgggacgaag acctgcgtga aatgaacaac cccatgccgc gatggtggat gtggttgttt
     6601 gtgctgaccg tcatttttgg tttggggtat ctggcggctt atcccggcct gggcagctac
     6661 aagggtcaac tgggctggac cacccacggc gaatacaccg acgaggtcga aaaggccaac
     6721 aaggagctgg caccgctgta tgcgcagttc accgcgaaac cggccgagca gctggctggc
     6781 gaccctgcgg ccatggccgt tggcgagcgt ctgttcatga acaactgcgc ccagtgccac
     6841 ggttcggatg cacgcggcag caagggtttt cccaatctga ccgatggcga ctggctgcac
     6901 ggcggtgcgc ctgccaagct tgttgaaacc atcactcaag gccggcaggg caacatgccg
     6961 cccatggccg cagccgtggg tacacccgac gacgtgaaaa acgtggccca ctacgtgctc
     7021 agcctgtccg gcagtccgca cgactcgttg cgttcccagc tcggcaaggc caagttcacg
     7081 gcatgtgcag cctgtcatgg catggacggc aagggcaatc aggccctggg tgcacccaac
     7141 ctcaccgacg acatctggtt gcacggctgg ggcgaacagg cgattgtcaa catggtcaat
     7201 agcggcaagg tcaacgtcat gccgggccag gcgggtaaac tgaccgaagc gcagatccat
     7261 gtgttgtcag cctacgtctg gggtatgtcc aacaaaccag cgacgctcgc gaccgcgcgc
     7321 ccctgaccac cgaaagagtt caccccgtgc aggaaacgcc agtccgcaag atcatcccca
     7381 tcgttcccga ggaagaggta tcgctgtacg aggcgcagaa gaagctgtat ccgcggtcgg
     7441 tgacggggct gttcgcccgc tggcgctggg gcttcgtgtt cctgacgcag ctggtgtttt
     7501 atggcctgcc ctggctggaa tggggccagc ggcaggccgt gttgttcgac ctgggtgcac
     7561 gccgcttcta cctgttcggc gtcgtgctgt acccgcagga cttcatttac ctgacgggtc
     7621 tgctggtgat cagcgcgctg tcgctgttcc tgttcacggc ggtggcgggc cggctgtggt
     7681 gcggctttac ctgcccgcag accgtctata ccgagatctt tctgtggatc gagcgcaagg
     7741 tcgagggtga tcgtagcgcg cgcatgcggc tggatgcgga gcccgcatcg gcgcgcaaac
     7801 tgctgaaaaa ggggaccaaa cacttcctgt ggattgtcct gtcgctgtgg accggcttca
     7861 ccttcgtggg ctacttcacg ccgatccaga ctttgtgggc cgaggccttc acgctgtcgt
     7921 tcggcccctg ggaatggttc tgggtgaact tctacgccat cgccacctat ggcaacgccg
     7981 gcttcatgcg cgagcaggtg tgcaaataca tgtgtcccta tgcgcgtttc cagagcgcca
     8041 tgtttgaccg cgacaccttg atcgtgagtt atgacgcggc tcgcggcgag ccgcgcggcg
     8101 cccgctcgcg caaggccgac ctgtccacgc tggcgttggg ggcctgtgtg gattgcaccc
     8161 tgtgtgtgca ggtctgcccc acgggtatcg atatccgcga gggactgcag tacgaatgca
     8221 ttggctgcgc cgcctgcgtc gacgtgtgcg acacggtgat ggacaagatg aattacccgc
     8281 gcggcctgat ccgcttcagc acccagaacg ccatggccgg ccagtggagc aattcccagc
     8341 tgtggcgcag ggtgcggcgc ccgcgggtgc tggtctacag tgcgatcctc ctggcgcttt
     8401 gcgtcggcat catgtggagc ctgcttgagc gtacgccgct gaaagtaaat gtggtgaggg
     8461 accgcgcagc actgtcgcgc atcgtcgccg gcggcaagct ggagaatgtg tatcggctgc
     8521 agatcatgaa tgccaccgag cagccgcagc gttaccgcat cacggcgtcc ggtctggaag
     8581 gtctggtgct ggcttcggag tccgaattcg atatcggccc cgccgaatcg cgctgggtgc
     8641 cggtgcgctt gcagatcggc tacgggtcgg ctgctcccgg atcacacgcg atcgcgttca
     8701 acgtcagcga cattgccggc gagagccatg tgaacgagaa gtcgattttc cttgttccac
     8761 gatgagggag agcaacatga aggaaaacta catgagtact gcccaggcac cgcaagcggc
     8821 acccgcgccc tggtggaaat tcggctatgt ctggctggtg attgcgggcc ccgctgtcgt
     8881 ggtggtggcc ggttttacca cgctctggct tgccctggcc aacccggaat cactggtggc
     8941 cgaggactat taccggcagg gcctggagat caacaagacg ctcgctgccg agagggaaaa
     9001 atccctcttg ccggccgtgc agggccgaaa tcatgcagcc acgccgcctg gtgccacgct
     9061 ggcgcctgcc ggacagcgct aactactata acgacggaga cagccatgtg gaaccagaaa
     9121 ctgatgtgga tcgcgtggcc cgcgttcctg ggggcgtgcg cgcttgagtt gcttgtcttt
     9181 gccgtggtcg atccggggga cctgctctgg tctggccagc cgctgggcct ttcgcggcag
     9241 ggcgtgtaca ccgcggcctt ttttgtgttc tggatggtgt gcatggtgtc gagcgcattg
     9301 accgctctgc tgggcatgtc gtccgccgag gtcaaccgct gtcctgtccc cacaggcgag
     9361 cgccctgaca gctgccgccc gcaataacac gaagcgaggt tgccttggca ggcctctgca
     9421 cggtatggtc atgaccaccc agttcaaaga ccgcgctgcc gccggtcgtg ccttgtccgg
     9481 tcatctgccg gcatatgcag gacgcgacga tgtggtcgtg ctggccttgg cacgcggcgg
     9541 tgtaccggtt gcgtgggaag tggcccgggc gctggttgct gaactggact tgctggttgt
     9601 gcgaaagctg ggtgtgccag gccggcccga tctggccctg ggcgccattg ccagcggcgg
     9661 cgcaaggtac attgatccca aggctttggc tgccagcggg gtgagtccgc agcagctcga
     9721 agagatcatc gcgaccgaga gccgtgaact ggcgcggagc gagacgcttt accgtgggtc
     9781 gcgggaagct gcacgcatcg aaggtcgcac ggtcattgtg gttgacgacg gtgtggccac
     9841 cggcgcctcg atgtgtgccg cactgatggc cttgcgaggg gccaagcccg cgtggatcgt
     9901 ggtggccgtg ccggtcgcgc ccccgggtgc gcaggagtgg atcggcacgg cggccgatga
     9961 gtttgtctgc gccctgagtc cgccggactt ctgtggtgtc ggaaactgtt accgcgactt
    10021 cggaccggtg ggcgacgagc aggtacgcag gctgctggct gcgtcctgag gacgctctgc
    10081 cgcgccagcc tggtattgcc gccctgcttc agtgagcgcc tggcttgctt tccctttcca
    10141 tctccgagcg gatcaccagc accggcaccg gggcgagccg gatgacctgc tcggcaccgc
    10201 tgcccagcaa catgcgcccg accccgcgtc gaccatgggt gccgacgacc accaggtcag
    10261 ctttccactg cctggcggcc tcggccaccg cctcggcgag gcgctccccc aggttgtcga
    10321 aaagcaccgt atccacctcc acgccagcgg ccttggcgat ggccatggcg tcattgagga
    10381 tctgcatccc ggagtcgcgc atgatccggg tcagatcgcc cgcatagccg ccaaactgtt
    10441 catacccggt caggtgggtc agttcttcca gcacatgaac aagccgtacc ctgccgccag
    10501 catccctggc gagctgcaac gccgcagtca gggctttgtt ggatgtatcg ctcccgtcga
    10561 cgggaacaag tatgcgctgg tacatggtcg attccttgaa ggctgtcgta gttggattca
    10621 gtgtgaagtg ggcgcagtgc aaggccattg acatgggtca acggcatgga ttgccggggc
    10681 cgggtatgta ggataagagg gcttggtaaa aagtctccga cacacggtca tggcctatgg
    10741 tgatgaatac ttttgataat tgctacactg gggtatggat tattctccta tcagcggcct
    10801 ggatgcagcc tctctggctg acaaccccgg cagcgtagca gcgttgctgg cgggggtgct
    10861 ggactcggcc atggatgcca tcatcacggt ggatgagcag caaaagatca ttctgtacaa
    10921 ccgcgcggct gaaaaaatct tcggatggcc acagcaggag gtcatgcacc agtccctgca
    10981 gaggttgatt cctggccgtt tccggcaaga gcatgccggg catgtcgacc gcttcggaac
    11041 catgggtgtg acctcgcggc gcatgggcac gcgcaatgtg gttcatggcc tgcgcgccaa
    11101 tggggaggag ttccctctgg atgcgtcgat ttcgcagctt gatacgccgg gcgggaagtg
    11161 gttcacggtg atcctgcgcg acatcaccgg ccgcttgctg gcggaagacg agcacgcgcg
    11221 gctcgcggcc ctgctggact ctgccatgga cgggatcatc accgtggacg agaagcagca
    11281 catcgtgatg tttaaccgcg cggctgaaaa aatcttcggt tggccggatg ggcaggccct
    11341 gggtcagcct ctgggcgagt tgattcccga acgctttcgc gccggccacg ctgaacacat
    11401 cagccggttt tcggcaactg gtgtcacctc ccgacgcatg ggcgacggct ccgtcattta
    11461 cggcttgcgc gccagcgggg aggaattccc gatggacgcc tcgatctcac agctcgacat
    11521 ggccgacgga aaactctata ccgtcatcct gcgcgatgtg accgagcggg tccgggccca
    11581 ggaggagcga agtgcctttg cggctgctgt gaatgctgcg cgtgaggaag aaaagacccg
    11641 ggtggcccgc gagctgcacg acgagctggc gcagtcgctg acggcgctga agatggacac
    11701 cctctgggtg cgcgacaaca cccgtgcggc gcctgacgtt gcggcggcca aactgacaga
    11761 tatgctgacg atggtggatg gcatggtggc ttccacgcgc cgcatcgcgg cggacctgcg
    11821 gccactgttg ctggacgatc tgggcctgct ggccaccatc gagtggctgg tccacaactt
    11881 cagccagcgt acaggcgtga cttgcacgct tttggccgac gaggacctgg agctgcagga
    11941 gccctatgcc actgccgtgt tccgcatcgt tcaggaggcg ctggccaacg tcgccaaaca
    12001 cgccggcgcg accgaagttg ccgtggttat cggcagcctg ccgggcgaga tcacgctgtc
    12061 ggtgcgcgac aacggcaggg gttttgtgac cgggatgccg cgcaagcggc agtcgctggg
    12121 gctgatgggt ttgcacgagc gggcgcaact gctcaacgga tgcgtcagca tcgacagtgc
    12181 gctggggcag ggcacgcgcg tcggcgtgac cattccgctg cccgaagagg aggccgcccc
    12241 gtgatccgga tcgtgatcgc cgacgaccac gccattgttc gcgaaggtct caaacgcatc
    12301 gtttcatcgg cagatgacct gcaggtggtg gctgaggcgg ccaacggaaa cgaggtgctg
    12361 cagcgtgtgc gcgaatccga gctggatgtg ctggtgctgg acctgtccat gccgggccgc
    12421 agcggcatgg aactgatcaa gctggtgcgc agcgaaaaac cgaaagtgcg gattctcgtg
    12481 ctcagcatgc accaggagct tcagtatgcc gtgcgcgcca tcaagtccgg cgccagcggc
    12541 tacctgacca aggagagtgc gccggggcaa ctggagcagg ccatccgcaa gatcgccacc
    12601 ggcggcgcgt tcatcagtgc agaagtcgcc gagcagctcg ccctgagtgc catgccgggc
    12661 ggaacctcgg cgccgcacga gtcgctgtcg aaccgcgaat tcgaggtgtt tcgcctgatt
    12721 gctgccggtg tttccgtgac ggacattgcc acgcgcctga aactttcagt caagaccatc
    12781 agcacccaca aggccaacct gatgcagaag atgggcctgc aaaaccagag cgaactgatc
    12841 cgctacgcca tcaaaaacgg ggtgtcggag ccgctggaac cctgagcccg gtcgtgaccg
    12901 gcgcttgacc ttcgtcaagc tgcatccggc gcctgtcccc taaggtggcg ggatcgcaat
    12961 gaccaaggag ccggcccatg aaaaccaccc tcacgcagct gctcgttcat ctggacgctt
    13021 cgccgcaggc cgcgcaacgt cttgaggcgg cgtgccgcat tggccaggcc cacggtgctg
    13081 ccgtgaccgg cctttacgcc gtgacaccca gtttcgtcgc gctgcccttc gcacccgagg
    13141 ttggtccggg cgtggcagcg gccctgcgcg agattgatga cgagttgcgc acgcgtgccc
    13201 gcgcaacgtt tgaccgcgtg ctggcgaagc ctggcatgca ggccgcctgg gccgaggtgg
    13261 gcgacgatcc catcatgggc gtttttgccc agcaggccct gtacgcggac ctgctggtgc
    13321 tcggccagca cgatccggcc agtacaccgg cgactggcgt gccagtcgat tttgccgagt
    13381 ccgtgatggt cgccagcggc aaaccggccc tgatcctgcc ttatatcggt gcatcgtcca
    13441 tcggcgacac cgtggtgctt gcctggaagc cgacgcgcga ggcggcccgg gcggtcagtg
    13501 ctgcgctgcc catgctgcag cgggccaggc gcgtgcatgt cctgtcctgg tcaggcgcgg
    13561 acgaggcggt gagcggccag ctgctggacc tggacagtta tctcaaactg cacggggtcg
    13621 aggcgacctg gcaccgcgag acgggggagc ccgagttgct cggtgagttg ctgctctcgc
    13681 gcgccttcga cctcgaggcc gacctgctgg tgatgggctg ctacggccac agccgcgcgc
    13741 gggagtgggt gctgggcggc acgtcccgca cggtgttgcg gtccatgacc ctgccggtgc
    13801 tcatggcgca ctgacggcgc ggtcacagca gggcgcttga aaccggccat gcccggggcg
    13861 tccttgcgct cggattattc ctagacgggg taggcgctca agtcttctga atccggattt
    13921 ctccgaggga tgcattcagc cgctgcctat ggccgcaggg cccggttgcg gccacagtag
    13981 agccatgcct tcccgccaag cccctctcaa agtttttatc gttgatgact cggtcctgat
    14041 ccgcgaccgc gtcgccgcca tgctcgaagc cagcgcgatc agcgtcgttg gacgggccgc
    14101 cacgccgcag gacgccattg acggcatcct ggcagcgtcg cccgatgtgg tggtgctgga
    14161 cgtccagctt gaaggcggct ccggtctgca agttctgcgc gccgtccacc cggccgcacc
    14221 agatatcgcc tttgtggtgt tcagcagcaa ttccggcccc gcctaccgta aacgttattt
    14281 cggggagggc gccgaagctt tcctcgacaa aagcaccgaa ttcgatcagc tcgttcagac
    14341 cgtggcgaaa gcctcgcggt atgttgcatg ttgacgcctg aatcacccaa ggagaaaccc
    14401 atgtctgcag ccaccctcga agccgtcgcc gcccccatcc gcatcgtgtc ccgccaagcc
    14461 gctcccgcaa ccgcagcggt cgcgcccctc atgacgcatt gctccacctg ccacctgcgc
    14521 gacctgtgcc tgccctgcgg catgacgggc agcgacgtgg agcggctcga cagcctgatg
    14581 ttcagccggc gcaaggtgaa agcggcccag acgctgtacc gtgaagggga tcgtttccag
    14641 ttcatctatg ccgtgcgtag cggcaccttc aagtccagcc tgacgctggc cgatggccgc
    14701 gagcaggtgg gcgggtttta catggcaggt gaactgatgg gtctggacgg tgtcgccaat
    14761 ggcgcgcatg ccagcagcac caccgcgctg gaagacaccg aagtgtgcgc cattcccttc
    14821 acccatttcg ctgaagtggc ggctggcaac ccgggcatgc agcacgtggt cacccgcttg
    14881 atgagccgcg agatcgtgcg cgaacacagc ctcatggtgt tgctggggag catgaatgcc
    14941 gaggagaggc tggcggcatt cttgctgaac ctgtcgcagc gcatgaaggc gcgcgggtat
    15001 tcggccagcg agttccacct gcggatgtcg cgtgccgaga tcggcagcta cctcggcatg
    15061 acgcttgaaa cggtgagccg gaccttttca gccttccagc aacagcgcct gctggaggtg
    15121 gacaaacgcc acatccgcat caccgacctg gacggcttga cccgtgcttt cgagacgcgc
    15181 gtgcactgaa cgcgtgttga gcggcgaatc ctgtcatgaa gtacaaggac tattacgccg
    15241 cgctgggtgt gccgcgtgat gcggacgtcg agcagatcaa gaaggcctac cgcaagctcg
    15301 cgcgccagca ccatcccgat gtgtccaagg cgcccgatgc agaggcccgc ttcaaggaag
    15361 cgggggaggc ctacgcgacg ctgaaggacc ccgaaaagcg tgcggcctac gaccagttgg
    15421 gccgccgccc tgccggggag gagttcgcac cgccgcccca gtggcgccag gatttcggtg
    15481 cggacgccca gggatttgag ggtgttgatc tggccgacct gctggctgcc atggggcggg
    15541 ggcgcaggtc atccgggggg ccggtgccga tgcatggtcg ggactacgaa accagcgtcc
    15601 gcatcaagct ggaagacgcg catcatgggg caagcgtcaa gctcgatctg gccgatgagg
    15661 aaggcggtcg cacgctggaa gtcaccattc ctgcgggtgt gaccgaaggc cagaagctgc
    15721 ggctgcgcgg caagggtgga aagggccgca acggcggggc cgatggcgac atttaccttc
    15781 acatcacgct cgcgccgcac cgggtgtttc gggccgaccg ccacgacttg tactttgacc
    15841 tggcgctggc gccttgggaa gcggcgttgg gcgccgatgt ggaggtgcca acgctggacg
    15901 gtccggtggt gctcaccgtg ccgccgggga cacattcggg tcgcaagctg cgcctgcggg
    15961 gcaggggcct ggccaatggc aggggcggcc gcggtgacct ttacgcggcc gtgcagatcg
    16021 acgtgcctgc catcctgacc gagcgggagc gcgagctctt cggggaactg gcgaaggtct
    16081 cgcgtttcaa cccgcgcgcg cccgcggaca aggagaagtt tcatggaaca acagctgctt
    16141 gacgtcgcgt ggctcgacgc acacgaggcc gtgaccctgg ctgaactcgc acaaatctgt
    16201 gccctgagcg tggacgaact ggatgaactg atggactatg gcgccctggt gccggtgcag
    16261 gacgtccggc aggagcgcct gttcagcgcc gaatgtgtga tgccgctgcg cacggccggc
    16321 aagctgcgcc aggatttcga cctggacctg ttcacggttg ggctcctgct gggctatctg
    16381 aaccgtatcg agggactgga gcggcaggtc cggtccttgc aggcgcacct gcccggcaac
    16441 acgcctgtgg aacggcgcta gatcagcgct cttttttccc gggaacgctg caaaactggt
    16501 cgtagagaac tttcatcacg tccagcgcct gggggctgtc gatgcggtaa taaatggcct
    16561 tgccgtcacg gcgtgtgctg accaagccct cgtcgcgcaa cacgccaagt tgctgcgaca
    16621 gcgtgggctg caggatgccg accgattcct ccagctcacc cacgcgcatt tcgccctgtg
    16681 ccagctggca caacaagagc aggcgatccg gattggaaag cactttcatc ag
//
LOCUS       DAIDFG010000030        16699 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_30_length_16699_cov_16.074982, whole genome shotgun sequence.
ACCESSION   DAIDFG010000030 DAIDFG010000000
VERSION     DAIDFG010000030.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 16699)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16699)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16699
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30_length_16699_cov_16.074982"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3001 a         5448 c         5409 g         2841 t
ORIGIN      
        1 ggcagcgtgg tcgccgaagt ggtgggcacc atgggcgcca tcaacgcctc ctcccgcaag
       61 atcgtcgaca tcatcggcgt gatcgacggc atcgccttcc agaccaatat cctggccctc
      121 aatgccgccg tcgaagcggc ccgggccggg gaacagggca gaggctttgc ggtggtggct
      181 gctgaagtca gaaacctggc ccagagaagt gcggcagcgg ccaaagagat caagactctg
      241 atcgatgact cggtgagcaa ggtcgaagaa ggcagcagcc aggtggccga ggccggcaag
      301 accatggacg agattgtcga cagcgtcaaa cgtgtgaccg acatcatggc cgagatcacg
      361 gcggccagcc aggagcagac ccagggtatc gagcagatca accaggcgat cacgcagatg
      421 gaccaggtga cgcagcagaa cgccgcgctg gtggaagaag cggcagcggc agcgtcctcg
      481 ctgcaggagc aggccagcgg actgagccag gtggtcagcg ccttcaaact gaaccagcag
      541 cagcaagccg cacagaattc ggcgcggcac acaagccaca gcggccagcg tgggacgccc
      601 atgcaaccgc cccgcgccag acaggccgtg gccggccccg cgccgcggca gttgagccat
      661 gccggtaccc tggttaccgc gcaaggaagt tgggaaacgt tttaagcgcg gcgctgcgtg
      721 ctgggtagcc cgcagcgccc cagccctaca ggaccggcgc gccctgcgcc ggaaaatcag
      781 ccttttttct cgaccgggtt ggccgtcagc cagttggccg ggtaggcgcg catgaactcc
      841 ctggctttct cgggacgcgc gctgagccag gcgtcggcgg cgccttcgtt caggatcgcc
      901 accatacgtt tttcgttgcc gggctgctgg tagcggctca tcagggcgtg gctgttggcg
      961 ttgaccgtga gcagggtgta gctgacgatg acttcaccat cggcgcccgt ccagctttcc
     1021 cacaggcccg ccacccccat gggtttgccg tccacgcggg cgatgcgggt ggacacggcc
     1081 ttgccactgc gccagtcgtc ttccatgaag gccatcatgg gcacaatgca gcgctgcccg
     1141 gccagccagg cgtcgcggaa gttgttggag gtggtcacgg tttccgagcg ggcgttgacc
     1201 agtttggtcg aacgcagctt ggcgtcggat gccgacttga cccagcgcgg caccaggccg
     1261 aactgcccgg ccaccagctc acgcaggggc gcctcacgcg cgggtttgtc gacgcctgcg
     1321 acctcttcaa cctcctcgat ctcttcgctt tccggcgccc ctgcggcctg cgccgcccgg
     1381 atgaacacac ccggttgacg cggccacacc ttgcgcccca ccggtgaagc gggggcggcc
     1441 acatggaagg cctcggggta aagcgtggcg gggtgaaggc tctcgtattg ggtactcatg
     1501 cgctgatgct atcgcagtgc ggccagcggg cgctgcagga cggccccgca agtcgcgtcc
     1561 ttcgccgaca cacaaggccg gtttccacgc gtaatatgtt ctctgcggcc tgtcagttgg
     1621 tgcaacactg cactcgcggc cattcagatc attcatccat ccctccagga gattgccatg
     1681 ccccagttcg ccatgattac cggtgacgtg acttacacac cgggtgacgg tgcgcccatc
     1741 acgattccga aggtgcgcgt ggaagtcgcg ctgtcgttcg acagcgccac cctgagctgg
     1801 gaagatgcgc cgggcatcgc cggcctgacg gccattcccc tcacgcaatt cgacgactat
     1861 gtgaaagacg gcaagatcac ctggataaag gaataaatca gcaagccccc tccgcatcgt
     1921 gcccacccgc cctgttcacc ccgcccccgc gctttttaac cgccgctgtg ccctgctggc
     1981 cggcatgggt ctgctggcct ggccggccgc gcgccttgcc gccgcgccag cttcgccgtc
     2041 gccgtcctcc tccggcgtct ggccgcgcgc gctggcggtg cccggcggca ttgcccggct
     2101 ctcgctgggc ccggcggcga cgcggccgaa ggcgcatctg ccgcacgccg acggcgacat
     2161 ccccttgctg gtggtgggcg acgtgatcga gtggacggcg ctggtgggca taccgctggc
     2221 ggccacgccc ggcgcggcga acatcgccgt gcaggcaccc gagggcggcc agcagcaggt
     2281 ggcctacacg gtggcgccca agcgctacag cgagcagcag ctcaaggtct cgccgcgcac
     2341 ggtggacctg tcgccggaag atctggcgcg ttacgagcgt gagcgtgcgc accaagcggt
     2401 ggtgatggcg accttcagcc agccgctgcc acaagccctg cgcatgcagg tgcccacgcc
     2461 ggggcggcgc tccagctcgt tcgggctgcg gcgcgtcttc aacgggcagg cgcgcagccc
     2521 gcacagcggc atggacatcg cggccggcac cggcacaccg gtgctggcac cgctaccggg
     2581 ccgggtgatc gacaccgggg actacttttt caacggcaac acggtctggc tggaccatgg
     2641 cggcgggctg ctgagcatgg tctgccacct gagcgccatc gacgtgcaat tgggcgatgt
     2701 gctgaaaacc ggcgaacgtg tgggcgccgt gggcgccacc ggccgcgtca cggggccgca
     2761 cctgcactgg ggcgtgatgc tgaaccgcgc catggtggat ccggcgctgt ttctggccgc
     2821 ctgaggcgcg gcggcgccac ccccgttcgc cctgagcttg tcgaagggta gccacaatgc
     2881 aaacaggctt cgacaagctc agcccgaacg gttaagcgca aatttacatg ccaaatcggc
     2941 ctccagccca atacctgagt gcgctggcag ctcctgaaag cgtagcagat tcccttcttt
     3001 ggcccggtcg ccgctgattc tggatgcccg cccctagccc attaggtcgc caacactagg
     3061 ggaaacccgc atgattgggc cattgttgaa ccactccccc aaggaaacac catgagcgcc
     3121 aaaaacctgt ctaccgttgc tgccgacctg atcgagtctt acggcaacac cgccaaaaac
     3181 gtgattgatg cctaccgcgc cggcggcgag cgtgtggtca gtgagctgga gcagcgctgg
     3241 aatcgcgctc tgaagcagtc gcgctcggag ctgagcgctg aagtcgccaa aaacgccacg
     3301 gcagcccagc tggccttcag cgcgctgtac agcaagggct tgaccctgag ctccgacggc
     3361 gccgagcagg tggtgagcca actggtgaag ctggccgaaa ccggtgtcga gcgcgctgcc
     3421 gccaacgccg cgctgtttga agaaaaaacc ggcgcgaaga cgctgacgac cctggcccag
     3481 gccaccgcac ccggcgccct ggcgctgagc aagctggccg cgcagatcga gcagaaaacc
     3541 gccgacctgg ccggcaagat cgccggcgac gacgccacca ccgtcaaagc caaacgcagc
     3601 tcggccttca gccagcggcg cgctgccaaa gccgcctgat acggttttag taaagaaaag
     3661 tggcaccagc ccttgagggg cgggcgccac cagctatcaa atatatagca tttgacgccc
     3721 gggaaagcag ccgccttcct gggcgtcagc ccttccggtc ctgtcagttc ttctccatcg
     3781 ccagcttgaa gcggcccagc gcctggtccc actggcgggc aatcagctcc agcgtgcggc
     3841 gcgcttcgtc aagctgctgc ggctcgaact gccacagccg ttcgcggcca atcttgaggt
     3901 ctttcaccaa cccggcgtcg gccagcacct gcaggtgttt ggtgaccgcc tggcgcgtca
     3961 gcgcggtgtt ggccgtgagc tgcgcaatgg aaaacacacc acccacacac agcgcggcaa
     4021 tcagctgcag gcgcgtcttg tcacccagcg caaaaaacac cggcgccatg tcgggcgcgg
     4081 gcgctgaggc cttgtcagcg cgcggtttgg ctggtgacat ggcgggcaat gttgtcgagc
     4141 tgatgcggcc agccgttgct gacgccccgg aaggcttcca tgcggcgcgc gggcggcacc
     4201 ttgtcaaagc cggtttcggt caccgtcagc agtgtgccgt cggctgcgtc tttcagggtg
     4261 aaggtcacca gcgtgcgctc ttcctttgaa tagtccaccc ccgcgtcgac cgcatggggg
     4321 tgccagcggt aactcatcag ggtttcgggc tcgacgcggt cgatcaccac gtcgaacacc
     4381 acatgttcat aaccctggat ggtgatgggc ccctgcacac gctggccggg ctcaaaacgt
     4441 ttgccggtga gcttggcgcc aaaccatttg ccgtattcct cggcgttggt gagtgaatgc
     4501 cacaccttgt tgcgcggggc cttgatgtgg atgctgcgtt cgacgcggtc ggtgctgctg
     4561 tcggtggtca taaggtgaac tcctgtcggt gaatgttagc ggggtctgcg gggcttgtgt
     4621 tcaggccgag caactcgtta cttccggaac gaaaggcgca ccctgcccgg tttcgaccag
     4681 gctttgcagg ctgccaaggt actggttcca gccgcgttcg cagaccgcaa aacattcgaa
     4741 gtccggcgtg aggccaatat gttcaaagtc cagcagggtt ttgccgccgg gctgtggcgt
     4801 gagccggaaa acgatgtcgg tgcccaccca ttcgtctttg cgtttgaagg cgtcgacgtt
     4861 gatcagcgcc tccacacaat gccagcgcac ttcacgcccg ggctccaggc tgttgatctg
     4921 catgaccttg tggtgttcgc caaaacggaa ggtggccttg ccgcccacgg tggtgtcaac
     4981 gtcgcagctc tgcgtccacc agctgcgcag gccgtgctgc gtggccacgg cctggtaaac
     5041 ggcggcgggt gaagcggcca gcagaagctg gcggtggtag tggttcatga aaaagctcct
     5101 tgcaggtaaa taggcaacca ttttgttgcg tttatattct gaaccagttt gacaaaacat
     5161 gcaaccaatt ggctgcgtga ttcaaaaccg catcgccggc accccgcgtg cggataatgc
     5221 gcagatgagc acaccgcccc cacctcctcg ctcccaggca ctcccccagg ttctggcttt
     5281 tgatgttttt ggcaccgtgg tggactggca cagcggcatc gcgcgcgaag tgcaggccct
     5341 gcgccccgct gtggacggcg ccgcgtttgc gctggcctgg cgcgccggct accagccggc
     5401 catgcgccgc gtgatggccg gcgagctggg ctggacgctg atcgacgacc tgcaccggct
     5461 gatcctcgac agcatcctcg gcgagttcgg cctggccgat ttgagcgagg cgcaaaagca
     5521 gcacctgaac aaggcctggc accggctgga cccgtggcct gatgtggtgg cggggctgac
     5581 gcggctcaag tcgcgcttca cgatctgcac gctgtccaac ggcaacatcg gcctgctcac
     5641 caacatggcc aaacgcgcgg ccctgccgtg ggactgcatc ctgtcggccg aagtctttcg
     5701 cgcctacaag cccgacccgg ccacctacct gggtgtggcc aaagtgtttg acctggcccc
     5761 tgccgacgtg atgctggtcg ccgcccacca ggacgacctg gccgccgccc gcgcctgcgg
     5821 cctgcagacc gcctacatcg agcggccggc cgagttcggc gcggcgcagc ccaaggacgt
     5881 gtcgcccgac cccgccaaca cctggcacgc gaaagacctg ctggccctgg cggagcagct
     5941 ggggtgctga ggccggaggc accgatggcg cacctcgcat ggcagcattc agcggcgaga
     6001 aaacccgcgc tcacggataa ttctttccca tgaacctttc cctctccctc ttccccctcg
     6061 gctggcagca ctatctgctg ggcggtctca tcgttggcct gggcacggcg ttgctgttcg
     6121 tgctgacggg ccgcatcggc ggcatgagca cggtgttttc cagcacctgg tccttcgtgg
     6181 tgcgctggcc gtttttccag caggcgcgtt ttgtcgggtc gcgcggctgg cggctggtgt
     6241 atgcggccgg gctggtgctc ggcgcgctgg tctggtggct gggctttgcc gacggcaagc
     6301 cggaagcgac tgcggtgccg gtgtggcagt tactggtggg tggcttcttc gtgggttacg
     6361 gcgcgcggct gggcaacggc tgcacctcgg gccacggcat ctgcgggctg ggttcgctgc
     6421 agctgccttc cctgatggcg gtgctgacct tcatggcgac cgctttcctg acggccaacc
     6481 tggcagcgag gtggctggca tgagcggcgc cgcacgcggc ctggccacgc tggcagcggg
     6541 cgccctgttt ggcttcgggc tgtcgtactc gaccatgatc cggcccgagg tggtgctgag
     6601 ctttctgcgc tttgaagact tcgggctcat gctggtgatg ggcggcgccg tcgtggtggt
     6661 ggcgctggtt tacaagctgg cgccccggct gctggcccgg ccgctgctgg acgaccattt
     6721 ccacacccac cccagcgcct ggaaccgcga caccgcggtg ggcgcggcgc tgttcggcgt
     6781 gggctggggc ctgtgcggcg tgtgccccgg cccggccatt gcgggcctgg gcgcgggcaa
     6841 ctgggacctg ctctgggcgc tggccggcat aacgctgggc gcgctggtgc agggactgcg
     6901 ggccaagtga ataacagccc tgcccgcctg atccttttca acaagcctta tggcgtgctg
     6961 agccagttca cgccggaggg gcgctggcgc gggctcaagg acttcattgc gattccggac
     7021 gtgtacgtgg ccggccggct cgatgccgac agcgaaggcc tgctgctgct gaccgacaac
     7081 ggccggctgc aggcccgcat cagcgacccg cgcttcaaga tggaaaaaac ctactgggtg
     7141 caggtcgaag gggtgcccga cgaggccgcg ctgcaggcgc tgcgtgcagg cgtgcagctc
     7201 aaggacggcc tcacactgcc ggccaaagcc cgcctcttgg cgccgccgcc cgaagtgtgg
     7261 gaacgcgagc cgccgatacg cacacggcag gccattccca ccgcctggat cgagctggtg
     7321 attcgcgaag gccgcaaccg ccaggtgcgg cggatgacgg cggccgcggg ttatccgaca
     7381 ctgcgcctga tccgcgccgc catcggccct tactacctcg acggcctggc cccgggaagc
     7441 ttgcgtgaac tggaacatca ggaagccgac agtccttgac tgaatgctgg tcaaaaatac
     7501 ctatattggg tatcatcgaa cccaatatgg gtatgaaaca agcaccaccc tttcccgcaa
     7561 gaagtctggc cgacgccctt ttccccaagg tgcgccaacg cgtgctggcc gtcctgttcg
     7621 gcaatccggc tcgcagtttt tacgccaatg aggtgattgc gctggcgcag tccggcaccg
     7681 gggccgttca gcgcgagctg gcggcactga cggaggctgg cttgctcgtc ttcaacaagc
     7741 agggcaacca gaagcactac caggccaatg cggctgcccc ggtcttcgat gagttgcgtg
     7801 gcctggtgct caagaccatg ggaatggccg acgtgctgcg tgtggccctg gcgccgctgg
     7861 ccgcgcagat tgaaggcgcc ttcatctatg gatcggtggc caaacacctg gataccgtcc
     7921 agagtgatat cgacgtgatg atcgtgagcg ccagcctggc ttatgccgat gtgttcagcg
     7981 cgctggaaag cgccacccac cgactgagcc gcaaggtcaa ccccacgctc tacaccccgg
     8041 cggatatcgc cagacgcatg accggggaca atgcatttct cacgcgggtc atgaagcagc
     8101 ccaaaatatg gctgatggga aacgaggaaa cactccatgt ccgaagcgct tgacaacttg
     8161 tgcggccccg gcaagcaact gcaagccgag gcgaccgatg cccaggagtt cgcgggcctg
     8221 gtacgtagtg gcatggcacg actgaacgac gcacgcaaca cctccctcgc gctggaaagc
     8281 cgctttgacc tggcttacaa cgctgcacac tcattgagtc tggccgccct gcgccggctg
     8341 ggctaccgcg cgagcaaccg ctacatcgtg ttccaggtac tgccgcatac gctggggctc
     8401 gggcccgagg tgtggcgcgt gctggacaaa tgccacaaca tgcgcaaccg gggtgaatac
     8461 gaaggcctgc tggatgtgga tgagcgactg gtagccgacc tgatcgtggc tacacaagct
     8521 gtcggtgagg cgttgccagc atgagcacgt cctggtacga ccgccacatc ctgccgcgcg
     8581 cgctggactt tgcctgcggc ctgccgatgc tgggccgcca gcgccagctg gtggtgccgc
     8641 tggcgcgcgg ccgtgtgctc gaggtgggcg tgggcacggg actgaacatg ccctattacg
     8701 acaaggcccg cgtcagccgc atcatcgcgc tggacccggc gctggagctg cacccgctgg
     8761 cgcttgagcg catcgcgcag gccgggctgg acgtggagct gctggcgctg tcggccgaaa
     8821 aaatcccctt gcccgacgcg agtgtggaca cggtgctgat gacctacacc ctgtgcacca
     8881 tccccgaccc gctggccgcg ctgaaggaaa tgcgccgtgt gctggccccc ggaggccggc
     8941 tgctgtattg cgaacacggc cgcgcgcccg atgcctcggt gcggcgctgg caggagcggc
     9001 tgcagccgct gtggggcaag gtggcgggcg gctgccacct ggggcgcgac atcccggccc
     9061 tgctgcagga agccggcttt gtgctgccgg acctgcacac acgttacctg ccggggccgc
     9121 ggcccttcac tttccactac tggggcgagg ccctgcccgc ctgaacacgg cctgaagtgt
     9181 ttactcggtg tcgcccatca ccaggtaacg cctgaccagg tcaaagaagc tgtcgtaaaa
     9241 cgcgtcggag ctgatggttt ggctgctgac cttgaccagc gagtcgttgc tggcactgaa
     9301 gggcaacgag accgatccga tcgcccccac cccaaggctg gccgagttgt tgctcttctt
     9361 cagggcgtag gtgtcctgca gggccgtgac aaaacccagg ctgaccttgc catcgatact
     9421 ttccggcgcg cacacgacac gaatcgcgat ttgaagatgc gcctcgggtt cgggctggaa
     9481 acttttctgg gcatccacca ggtctttcct ggcggtgttg atgaggtagc cctggctcag
     9541 tagcgcgcgc cgggatgcct cgcaggtctg cgcgggcgtg gcgtcgaaca tgcgcgagta
     9601 ggtggcggtg gatgcgaact gctcctgcaa gggaaactgc ttgatcgggg ctgcgcagcc
     9661 tgccagcagg ccggcgagtg cccacaggcc aagccggccc gcaggtggga aacgcatgca
     9721 aaacaggttc aaagccggta ctccttggca tcagtctaaa cgacggagtt gcgcgctgcc
     9781 gcctcgggct ggtgtatcgc gccgccggtg ttcgcaaccc gcacgcaggc aggctacggg
     9841 gcagggccgc ggcggccctg ctgcaacacc aggtcgtgca gagaaaccga tgccaggaat
     9901 tgcgccatct cgcgttcaag atcggcacac cagtcgctgg caatccgctg acccgcagga
     9961 gagtcggggc tagacggctg ggctgcggcg cgggtgcacg gaggctccga ttcgtcaacg
    10021 gcgtacacga tatcggccac ggtaatgtca gcggcgctgc aggccaggcc ataaccgccg
    10081 cctggccccc gcgtgctgcg caccagaccg cgccgacgca gccgtgcgaa tagcgactcg
    10141 agatacgtca gtgagatctt ctgccgtacg ccgatagtgg ccagcgcaat cggtttctga
    10201 tcggtgcgaa gggccaaatc cagcatggcg atcacggcca gacggccttg ggtggcgatt
    10261 tgcatcatgc actttcagtt gttgtttcca gaatcagaac ttctcggggc gcaagacgac
    10321 gacctcgcca acgtacatca ccatggagta gttgtcgtag tagcgctcgc gcagtacggt
    10381 gcagatggcc tgggccgtgt cggcgtcgca gagcacttcg aggcggatgt tggcgtgcgg
    10441 cgcccaggtg gcgtcgcgca tgccgcgacc gcccttgccg cgtgcatcgg tgatggtgta
    10501 gccacgcgca ccgaggtttt cgatgtcgtg cacgagcgtg tcctcaaggg cggactctgt
    10561 cacgatggtg agcagtttgc gggtagtcaa ttccatggcg tcaggctccg gcgtgatgaa
    10621 ggtgctgggc aagccggtgg taaagcggaa tgcccaccac gaggttgaac gggaaagtga
    10681 tgccgagcgc cgcgccgatc gacagcgccg gattggcctg aggcacggcg atacgcatgg
    10741 ccgccggcgc agcgatgtag gaggcgctcg cgtacagcgt ggccagcagc gtgacgccgc
    10801 ccacagacag cccgagcagc atgccggtga ccaggcccag gcctgcggcc gccagcggca
    10861 tgaggacggc aaatgcgacc aggaaaaagc ccgcctggcg caggtcgcca aaacgcctgg
    10921 caaccaccag ccccatctcc agcaggaaga aagcaagcag tcccttgaac aggtcgaaga
    10981 acaggcggtc gagcggcgcg atgccgcccg gccctgccac ccagcctatc accagcccac
    11041 cgatcagcaa cacgatgctt ttgccggcga agacctcgtg tactacctga cgccaggggg
    11101 tgccttgctc gccgcgccgc gccagcagca caccgatcat caaggcagga aattcgagca
    11161 acaccaggaa cacggtcatg tagccctcgg cggcttcgcc cagccgggcc aggtagctgg
    11221 cgccgacagc gaaggtgacc acgctgaccg atccgtagtg cgcagcgatc gatcccgcat
    11281 cggcgcttga aagcttgccg acgccgcgca ggaccggaaa tgcgaccagc ggaatcaggg
    11341 cgcccaccac caccacagcg agggccggca gcacgacatc gagcagcgga taacgcgcga
    11401 gctcgacacc acccttcagg ccgatcgcga gcaggagaaa gatgctcaag gcctcataga
    11461 ggacgccggg gatcttgagg tccgaacgca cgaaaccagc gatcgcgccg agcacgaaga
    11521 acaggatgac gggttccacg gagggcatcg ccagcggctg ttaccgcaca atcagcacgg
    11581 gcagcctggc caggtgcacg acttggtagg ccaccgagcc cagcaacagg gattcggtgg
    11641 cggtcagccc gcgggagccg atggcgatgc cgcgactgcc caaggcatcg gccaggcttg
    11701 tgatctccgg cgcacccacg cccatgacca catgcacgcg ccacggaacc gacgccgcgt
    11761 ccagcaactg gcgcgcctgc gccgtggctg cccatccgcg ccgcggcagt tcggtctccg
    11821 cggcctcttt gttcagccag ggctggacat gcaccagatc aaccccggca cctttctccc
    11881 gcgtcgccag ctccgcgacc atcgcgacgg cgcgcaacga gcacgccgag ccatcaaccg
    11941 ccaccagcca gcgcgcgcta ccgttgtcgc ccgtggcgcc agcgccgtgc caggtcgtgg
    12001 caacgagccg gcccacgtca tcgcgccgct caagggctgg tgcgggaacg gcgtcttgta
    12061 tcgcagcatc ggtcatgaat cactcctgga aggttggcgg gcagtcatct tcaggctgag
    12121 gactatggaa acgagaatga tggagcccac gatcaacagc gaccactgaa ccggcatatg
    12181 gaaccagggc atcgccagca tcttgcctcc gataaagacc agcaccagcg ccaggccgta
    12241 cttgagcaaa tgaaagcgtt cggccatgtc agccaacaga aagtacagcg ccctcagtcc
    12301 catgatggcg aagatgttgg aggtgaagac gatgaacgga tcggtggtca ccgcaaaaat
    12361 ggccgggatg ctgtccacgg caaacaccag gtcgctggcc tcgatcatca tcagcaccag
    12421 gaacatgggc gtcgcataac gcaggccgtt gatgcgcaca aagaaagcct cgccatgaaa
    12481 gcccggggtg atgcgcatgt ggcagcgcag ccagcgcagc aaggggttct tctccagatc
    12541 gggcttctga tcggccatga ccagcatctt gatcccggtc accaccagaa aaaagccgaa
    12601 cacgtagagc acccaggcaa actccgacac cagccagacg ccggccatga tcatcccggc
    12661 gcgcatgaca atcgcgccga tcacgccgta caacagcacg cggcgctgca actctggcgg
    12721 aacggcgaag taggtgaaga tcatcacgaa cacaaacatg ttgtccaccg acagggtctg
    12781 ctcaatcagg tagccggtga agaactccag ggccttgcgg ttggcgatct cgcggccggc
    12841 cgtgccgtcc aggtaccacc acaacaaggc gccgaacgtc gtggccagca gcatccaggc
    12901 cacgacccag cttccggcct ccttgaccga aacacggtgg gccttgttac cgcccaggac
    12961 aaacaagtcc agggcaagca tggcaacgac gaaagcgatg aagccggccc acatcggacc
    13021 ggtggcgaaa gattcaatat tgctcatggg cccctccgga cgtggcatgc gtggcgggtc
    13081 ttcttcgtaa gggcgtggtt gaggcgcttg ttttcatggg gtgtcaggtg gtgttacagg
    13141 tcagccggca agaagccggg aaggtttggg gcggcataac gccgggcgac cgcgtcgcaa
    13201 gtggctggac cctgccgcgc gcggaaatcc tggcagggtg attcgcgcca ttcggtccaa
    13261 ctgagcctca tcagtcggcc gccatgccaa gaaccaggag tcccgcaccc acgcctgccg
    13321 gaatgcccat acgctccagg cccgaggccg cgtccccggc aatcacctcc acgggcaccg
    13381 tcgtgcgttc gatcacctgg ttggtgacgg agtcctcgac ccagcgaacc agcgaactct
    13441 tgcgcgcggt gcccatcacg atgcgatcgc atcggagccg gcgtgccgca tccgcaatgc
    13501 aaacggccct gtctccgact tcggtatgga cggtgtaggg aatgccggag cggtccagcg
    13561 cctgccgcgc cggcacgagc gccttttcgg cctgctcttg atggaacgcc atccggtccc
    13621 gcctgccggt gaaacgggcg acctcactcg aaaacggccg ttgtatgttg agcacatgaa
    13681 tttcgagcgc ccggttcttc atgaattccc tctcgacgtg acgcacgccg tgcagagaat
    13741 tgggtgatcc atcgaacggg acaagaactc ttgacatggc tttactcctt cagttgattg
    13801 gtttgacgtg gcggtattca agggcctgtg ggttcaggcg agccacactg cagcagccac
    13861 cagaactccg ctcgcatcgc aagcgcccac gcgctttgca tctcgcctgt tttcggcgag
    13921 ctgttcgcgc caggcgcacc acaggatcaa ggcaggcagc gctgcgaaag gaacggcgta
    13981 gtcacccatg ctggccccgc atctccagca tctcgcgctg cagtccccaa tgctcggccc
    14041 cggcctccag cacgatcgat tcgctgtctg caacgttccc gtccgcttcc accatcgcaa
    14101 cgcacagacg gatgagcttg cggcgcagct caggccggtc gatctccgcc atcagttcgg
    14161 ccagcgtcct cgggtcaata cgacacatgg cgccccagct ggaatgcgct gccgagagga
    14221 gatcctcgca aagagtctgg accaccgagt ggaactcggc ctggcccatt ccgatctgcg
    14281 cgggcacgtc cagccggtca aagatgtcga gttccttttt gcacagactt ccgtcagcaa
    14341 gcatggcaag cgcaacaatg cgagccgcgg cctgagggct gtttcgggga taacttctca
    14401 tgggatgtac tttctgcttt tgcagggacg ttcagtcgaa aatttcgctg agccaggact
    14461 tgtgccggcg cggatagccg cgcttgccgt gccggtagtc cgagtccaca aagtcggggt
    14521 ggacctgctg cacctgctgg accggctgca cagcagcagg ccgcgcggcg ggcgctgcgg
    14581 cagaaaggtc cagcagcttg tcgagctctc cgcggtccag ccagacaccc cggcactggg
    14641 ggcagtagtc aatttcgacg ccctggcgtt cggacatcag cagggttgca tcggggcaga
    14701 cggggcattt catggggtgt tcctttcggg ggttttcata cggcagcgct cagcgctggt
    14761 gagtgatggc gcgcgtgcgg gtacggatgt gggtgggggc tggctgcaac ttgcgcagca
    14821 acgcccgaat tgcgcgcgac aaggcgcttt gcagcgccga tccccagtcg cgggtgatcg
    14881 acgtgaccac caccgtgccg gcgccttcgg cagtcaactc gacctggcaa cgtttgtcga
    14941 taccgtccgt ggcacctggc acgtccgaca tctgcaccct ggcgcgcggc gcgagccagt
    15001 cgagcgggcg cgtggcctgg cgcacccggc gcacggcaaa cccgcgcagg cgctccagcc
    15061 agggttcgcg cgattcaaaa ataatctcca tgggaggctc cttcactgtg gaagccttga
    15121 cgataccgcg atgtgtactt ctttaaaagc agataatggc gaaaaaacac ttctgaaaaa
    15181 cagaatcatg agcaaagact tcaactaccg ccacctctac tatttctggg tcgtggccaa
    15241 ggaaggcggc atgacacgtg ccgccgaccg tctgggcatg gcggtgcaaa cagtgagcac
    15301 tcaggtacgc gaactggaac gttcgctcgg ttacgccctg ctcaagcccg agggccgcgg
    15361 cgtggcgctc acccccgccg gcaccgcggc catgcgccag gccgagcaga ttttccagct
    15421 cggcgaacaa ctgccaagcg cggtgcgcga ggctgccagc acgccgaccg tgcggctggc
    15481 ggtgggcatc tccgacggct tgcccaagct ggccgtgcgg cgcctcctgc agccggtcat
    15541 gcatgagccc agactcaggc tgctttgcca cgaggacgag ttcgaggacc tgctcggtga
    15601 cctggcgctg caccggctcg atgtgatcct ggcggaccgg cccgcgccgc ccaaccccaa
    15661 cgtcaagctc tacagccacc cgctcgcgtc atcgccgctg gcctggtatg cgccgccttc
    15721 cctgtatgcc gcggcgcgca agggtttccc gcagtcgctg gcccgggtcc cggtgttgct
    15781 gcccaccagc cacagcgcgg tgcgcgcccc cctcgaccag tggttcgagc ggctggacat
    15841 caccccccac atcgccggcg agtttgaaga cagcgcgctg ctcaagacct ttggcgctgc
    15901 cgggatgggc gttttcccgg cggccgagct gatgcaggag gaaatgaccg cgcgttacca
    15961 ggtcaggcgg gtggggcctt gcgagggtgt ggaagaacac ttctacgctc ttgccgcgca
    16021 caagaagctg ctgcacccgc tggtgcaaaa gctgctggcg gccgcccgcc cgcgtcacgc
    16081 ctgaaccgga agcgggaagc ggacccctgc ggggctggcg gtgcgttgcg cagttcgcta
    16141 gaattcgcag gtgtgggggg gtagctcagc tgggagagcg ctgcgttcgc aatgcagagg
    16201 tcgggagttc gatcctcctc ctctccacca aattttcttt cacttcgtca gcttcatgca
    16261 tcccctcccc caccgggccg ccaccacgcg gcgccaatgg ctgcttgtgt atcgggccgc
    16321 aatcgtggag caagcgggcc acgatcttgg agtagggaaa agctgtggct ggcagggcag
    16381 ttgtccacaa attaccccct cctgggtatc cggctcccgg cggggcttaa aacgcccatt
    16441 aaacccgatg ttttgcgcgc tcttcggcgg tcagggcgtc caggctgctt agggcctcga
    16501 tcagccgccc ggtcgttgca gtcaggtcgc cgaggtagcc ccagttcttt ggctttgcag
    16561 ccgctgcatc tgcgtgggtg gccagggcgg tttccaagcg cttcaggagc tgctggttgc
    16621 gcgcctgttg cgcggcatag gcggtggcca gcgtcttcat gcctgacctg ccttggcggc
    16681 ttccgcgccg gcctgataa
//
LOCUS       DAIDFG010000031        16532 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_31_length_16532_cov_17.284761, whole genome shotgun sequence.
ACCESSION   DAIDFG010000031 DAIDFG010000000
VERSION     DAIDFG010000031.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 16532)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16532)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16532
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31_length_16532_cov_17.284761"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3122 a         5123 c         5336 g         2951 t
ORIGIN      
        1 gacagcctgg tgacgggcgc aacgctcgat accggcctgc agaaagcact cgaagaagcg
       61 attgccaagc tgcccatccc caaggtgatg agctaccagc tggagaccga ctgcgagttg
      121 cccggctgga gcagcgtgaa cttcgtgcgt cccgcgcatg gcctggtggc gctgcacggc
      181 agcaccgtgg tgccgatcac ggcgctgggc ctgaaggcgg gcaacaccac gcagggtcac
      241 cgctttgaag ccaaagtgtc gcccgtggtg atcaaggatg ccgacgcgta cgctcagacc
      301 ctgctgagcg acggcgcggt gatcgccagc tttgacgacc gccgctttgc catcgtgcag
      361 cagctcgcgc aggctgccga gcgcctgggc ccgggctacg aggtgctgat ggacgaggac
      421 ctgctgagcg aggtgaccgg gctggtcgaa caccccaacg tgctggtgtg cgcgttcgaa
      481 tcccagttcc tcgacgtgcc gcaggagtgc ctgatcctca cgatgaaggc caaccagaag
      541 tacttcccgc tggtcgacgc gaagggcaag ctcaccaaca agtttctggt ggtcagcaac
      601 atcagcccgg acgacgcgag cgccgtgatc ggcggcaatg aacgcgtggt gcggccgcgc
      661 ctggccgacg ccaagttctt tttcgatcag gaccgcaaga agacgctggc ttcgcgcgtg
      721 gcagggctgg acaaggtggt gtatcacaac aagctgggct cgcaggggga gcgcgtccaa
      781 cgcgtgcgca cgattgccga agcgattgcc aaccggctga atgccaatgt ggccgacgtc
      841 cagcgcgccg ccgaactggc caagaccgat ctggtgaccg acatggtcgg cgagtttccg
      901 gagctgcagg gcatcatggg acgttattac gcgctcaacg atggcttgag cgccgaggtg
      961 gccgatgcga tcgaggacca ctacaagcca cgttttgccg gtgacgagtt gccgcgcaac
     1021 accatcggcg tgatggtggc gctggctgac aagctcgaaa ccctggtggg catgttcggc
     1081 atcggcaatc tgccgaccgg tgacaaggat ccgtttgcgt tgcggcgcca cgccctgggc
     1141 gtgatccgca tgctggtcga gaaagatctg ccgctggggc tggccgagct ggtcgaggac
     1201 gcgacgccgg tctttgggga caagatcacc aacgcctctg ccgcgttgac cgacttcatc
     1261 tatgaccgcc tcgccggctc cctgcgcgag cagggcttca gcgcgcagga agtcgaagcc
     1321 gtgctggccc tgcgcccgca acgccttggc gatgtcgcca agcgcctggc cgcggtgcgc
     1381 gccttcgccg cattgccgga agcgccggcc ctggccgctg ccaacaagcg catcagcaac
     1441 atcctcaaga aggcccagga cgtggatgcc catgtcagcg aggtgctgct caaggagccc
     1501 gctgaagtcg ccttgtacgc cgccatgcaa gagctggcac ccaaggccaa cagccagttc
     1561 gaagctggcg actacaccgc ctcgctgcag acgctggccg cgctgcgtgc gccggtggat
     1621 gcgtttttcg acggtgtgat ggtcaacgcc gaggagatgg acttgcgcct gaaccggcag
     1681 ggcctgctca aaagcctgca cgacgccatg aaccgcgttg ccgatctgtc caaactcgcc
     1741 agctaggcga atcgtcatga aactcgtcat cctcgaccgc gacggcacca tcaacagcga
     1801 cagcgacgat ttcgtcaaga cgccggatga atggatgccc ttgctgggct cgctcgaagc
     1861 gattgcgcgg ctgacccacg ccggctggca tgtggtgatt gcctccaacc agtcgggcct
     1921 ggggcgcggc ctgtttgatg tggcctcgct caacgccatg cacgccaaga tgcacaagct
     1981 gctcgcggcg ctgggggggc gcatcgatgc ggtgttttac tgcccgcaca gcccggaaga
     2041 aagctgcacc tgccgcaaac ccctgcccgg attgttcgag cagataggcg cgcgttttgg
     2101 cataccgctc aagggcgtgc cgacggccgg cgactcggtg cgcgatttgc tcgccggcgc
     2161 tacggccggt tgcgagccgc acctggtgct gaccggaaaa tcggcaaggc accgggacgg
     2221 tggcctgccg gagggcctgc cgcccggcac gctggtccat accgacctgc cggcctttgt
     2281 ggaattcatc ctgaaccgca gcgccccagc cagtgctatg aaaaacgtag ctcctggcgc
     2341 agatgtttaa agggctgccg ccctgaaaca ctcgaaattt cgactgaaga cacacgccat
     2401 gcccctgatt cgctccgtcc tgcaccttct gtggatgatt ctcaccgtga ttccctgggg
     2461 gctggccgtc ctgatttttt cgctgtttgg cagcagcacg cagatttact ggctgtgcgc
     2521 cggctggctg cgcgtggccg tccgcggcgg cacggtgatc ctgggcatcc agaaccgcgt
     2581 gaccggcatg gaaaacctgc cgaccggcga gaccagcgcg gccgtgctgc tgctcaagca
     2641 ccagagcacc tgggagacgt tttccatgcc gacgctgatg ccgcacccgc tggcctatgt
     2701 gttcaagaaa gaacttctgt acgtgccctt tttcggctgg gccatggcgc gcatggacat
     2761 gatccacatc gaccgtaacc agcgcacaca ggccttcaac aaggtggtcg agcagggccg
     2821 ccggctgatg agccagggtg tctgggtcat catgtttccc gaaggcacgc ggattgaacg
     2881 cggaaaaaaa ggcgtgtaca agaccggggg cacgcggctg gcgatcgaga ccggcgcgcc
     2941 ggtgattccg gtggccgtga catcggccaa gtgctggccg cgcaaggcct tcatcaagaa
     3001 gccgggcatc gtcgacatct cgatcggcaa acccatcagc agcgtgggcc gcaagcctga
     3061 ggagttgatg cgtgaagtcg aggcctggat agaagccgaa atgcgccggc tcgaccccga
     3121 ggcctatccg gaaaccgcct cgggcgcctg acggctgggc tggtcaaagc tacactggtc
     3181 agcatgcaac ggcttcttca gttcacgctg gacctttttg acctggagcc agccagaccc
     3241 acggcagatt tgcctgccaa agccacaact tctaggccaa atgagccatt ggccattatt
     3301 gcgcctgcgt caacagctat cgaaaagata gcgtctgcat caaaccatcc acccgcccag
     3361 accctggcgc aggtgctggc gccggccact taccggcacc cggccgcttc tcgggaagcc
     3421 ctgctcgaag gcacggtggt gggctaccag ttccggcgtg gcaagcggcg caccatcggg
     3481 ttttcggtgg gggctgaggg cctggtcgtc agtgcgccca agtgggtgcc cctgtatgaa
     3541 atagacaagg ctgtgcagga aaaatccggc tggatcgtca agaagctgca ggaaacccgc
     3601 gcgcgccacg accggctgga gtcggcccgc attgagtgga aagacggcac gaccctgcct
     3661 tttctcggag agccggtgat tgtggtgatc gatccgcggc acgcctttgg cggtgtgggc
     3721 ggcgagctga aaagcagtgc tgctgctgag ttgcacaccg gggaagacgc acccaattcc
     3781 agcctgccgg gtgttccccg gctgaccttg cacattgccc tggcccacga cgccacggcc
     3841 gagcagattc gtgactcggt gcaggcctgg ctgatgcgcc aggccaagcg cgtctttacc
     3901 gagcgcctcg accatttcgc accgcatctg ggtgtgcaat ggcgcaaact cagcctgagc
     3961 agcgccggaa cccgttgggg cagcgccagt gcagacggct ccatccggct gaactggcgg
     4021 ctgattcact tcaaacagtc agtcattgac tatgtggtgg tccacgaatt gagccatttg
     4081 agggtcatgg atcacagccc gcggttctgg gacacggtgc gcacggtggt gccggactat
     4141 gcgctgctgc gcagccagct gaaggaagag gcactacccc gatggtaatt tgggcggcgc
     4201 cggggtctaa tgcggagtgt ttttgacaca tcacaaaacg cgcgccgaac gggctggata
     4261 aatgtgtaaa tgtctgtaat atgaagaata tgataaatat gacacgattc agcagggagc
     4321 tgccatccgg cttcaaaagg cctttatgaa cgataaacct attcttgtcg ttgaagacaa
     4381 ccccgaccac ttggaactca cggtcctgac tttgcaggag cagggggcgg acacggccat
     4441 cgtgaccgca cgcgatggcg ccgaggcgct ggattacctg tttggccagg gggtgcacgc
     4501 agggcgcgac acacagaagc agccggcctt tgtcctgctc gacatgaagc tgcccaagct
     4561 gtccgggctg gatgttttgc gcagcctgcg cagcaacccg ctgacggcgc tggttccggt
     4621 ggtcatgctg acctcgtcga gtgagtagtc cgacatcatt gcctgctacc agagcggcgc
     4681 caacggcttt gtgcgcaagc ccgtcgattt cggcgatttc acggaaaagc tcagccgtct
     4741 gcaggactac tggctgcgcg tcaacgagtc catcgccgca gcttgacggc ccttgcggcg
     4801 aggagggaac cttcgctgga gtagcggggg ctactgcaga tgacttacag tcgggtgagt
     4861 ccatctgaaa gcggccgctg gcggcctgtt tcagaaaccc cgaccgcatg aacttcgtcc
     4921 tgactccttt tcaaaccttt cagccccgtt ccacgcgtac ctgtccggac gcggcccttt
     4981 tacgtcgacc cgtgccgcag cggatcaccg catgagggtg gacatcagcc tgcgcacgcg
     5041 cattgtcgtg ctggtgctgg tggcgattgt tccgctgttc ggcctgtcga tgttcaaggc
     5101 actgcacaat gccggcgctg agctcgaacg cgccaaggcc aacctgcagt ttgcagcttc
     5161 gctggccgcc gccagccagg agcgggtggc agactccgcc aggcaggtgc tgaccctgct
     5221 tgcttcgctg ccggaggtgc aggaaggcaa tcacgccgac tgcgaccacc atttttcaag
     5281 cctgacccgc catcttcccg aatacgccaa tctgggcgtt ctggggctgg acggacaaac
     5341 ccgttgtcac gcgttgggga gcgccaagaa aacctttctg ggcgaccgca gctatttccg
     5401 cgatgccatc gcccagcgtc gctttgtcgt gggcacctat gcggttggac ggcttgccgg
     5461 cacgccggtg ctgacctttg cactgcctgt catgggcgcg gatgacaagg tgacgggggt
     5521 ggtctacgcc gccttcgatc tggccaggat ggccgggacg atcgcgggga tacagctgcc
     5581 gtctggggct gcactgggtg ttcaggaccg cgaaggcgcc ttgctggcgg gcagaccggc
     5641 cctgcctatc cgtgtaggcg acaaggttcg cagcccggta ttgcaggaag cggtcaaagc
     5701 catgagcacc ggggtgcgcg aaggcccgga tggcgggggg catgaacgtt tgtgggcatt
     5761 catgccgagc agcccgaact cggaggccgc ggtttttgtg tcggtcagca tggaccgcga
     5821 cctgatcgtc gcgcctgtcc ggcgccagtt gtggcaggag ctggcggcgc ttgcgctggt
     5881 ggcgcttctt ggcggcggga ttgcctggat gattgccagc cgttcgattg tggcgccggc
     5941 ccgccagctt cttcacgcca cgcgggagct ggaaaagggc ctcctggacg cgcgtgtgcc
     6001 catgagaccg ggtgagagtc agagtgaatt ttccagaatt gccgcgggct tcaatctgat
     6061 ggctgactcg ctgcagcatc gcgaaagggc tctggaaaat gagctggcgc ggaaccggca
     6121 ggcttatgca gcgctggagc tgaccatcaa cagcatgcag gaaggcctgc tggcggtgga
     6181 cgccgccgga cgggtgatcc tggtgaacca gacagcctcc cagctgtttg ccattgatga
     6241 cagctccatg gtgctggcgc cgcagtggcc gcaacgccag gggctgtttg ttccggacac
     6301 gcagggcttg tacggtttcg aggacctgcc cctgcacaag gccctgcgcg gcgagagcgg
     6361 cggcccccta ctcatgctgg tgcgcaacga gcgtgagccg gtgggccgcc tgatcagctg
     6421 cacctaccgt cccatgcagg gcgcgggcgg catcgttggc gccctgatgg tgttttcaga
     6481 catcacccgc atgcagcaac tgcagctgga gcagacgcac agttacgagc aactcagcga
     6541 ggtccagcgc aagctgctca atgcccagcg gctggggcgg atcggcaact gggagttgaa
     6601 cctcgccacc ggccggctct ggtggtccga cgaggtgtat gaactcttcg gtctgacccg
     6661 ggcgggcttt gacgggcgac cggacaccgt gattgcgatg gtccatccgg aagaccgtgc
     6721 tggctacatt cgcgtgcggg aggccgccat gcaggagggt cagcgcatgg acgtggagta
     6781 ccgcatcctc accgtggacg gccagacccg ctggatacac cagctcggtg agtcccatgc
     6841 agacagcagc ggccagatcg tctggcgtgc cggtgtggtc caggacatca cggcccgcaa
     6901 gcaggcggag ctcgccgtgg ctcgccacac ggagctgctg caacagactg gcgagatggc
     6961 ccgtatcggg ggctgggaac tgaccgtgga gaccatgacg ccttactggt ccgcagaggt
     7021 gtaccggatt tttgacctcg atccgtcggt gccattgggg ttgaagcagg cactcgagtt
     7081 tcacccgccg gaaatgcagc ccctggcgcg cgccacgctg gccgcggcgt tggccgacgg
     7141 cacgccctgg gacatggagg tgccgcttgt cacggcgcag gggcgacgca tctgggtgcg
     7201 cacacggggc catgcgatgt tgagggacag caaggtcgtg cgcctcatgg gggtgttgca
     7261 ggacatcacc gaccagcacg aatcccaggc gcaactccga ttgctggagg ccgccatttc
     7321 ccggctgaac gacatcgtgc tgatcaccga agccgatctg ctcgatgagc cagggccgcg
     7381 catcgtattc gtcaacaacg cgttcgagcg ccgcactggt tacagccgcg aggaagcgct
     7441 gggccggtcg ccgcgttttc tgcagggacc gcgaacccag cgcaccgaac tggatcgcat
     7501 cggtgcggcg ttgcgcaagg gggagtccgt gcgcgcggag ctgatcaact acaaaaagag
     7561 cggtgaagag atctggctgg acctggacat ggtacccatt gcagacacgc aggggcgggt
     7621 gacccacatc gtcgccgtag agcgcgacat tacccggcgc aaacttgccg agcaggccat
     7681 gaccgacagc gagcagcgtt atgcagccct gttcaaggcc gcgcctgtcc cgatgtggat
     7741 cgttgatgcc gggaccgccc gttttctcgc cgtcaacgag gctgctgtgg cgaactatgg
     7801 ccattccaag gaagagttcc tgcagctgac gctctttgat atccggtcgg gggcagagca
     7861 ggcccggttc aagcagcatc tcgacaaggg cgtcatcggc cagccgggcc gctggctgca
     7921 tctgcgcaag gacggcgttg agtttcccgt ggaggccgtg gcccgggcca ttacctacat
     7981 gggccgggag gcgcgtttcg cggttgcgct ggacatcacc gcacaggtca gggccgaaaa
     8041 ggaagtacag gattacctgt tcacgctgca gcgcgccgcc gacgcgaccc atgccatcac
     8101 gcgccaccgc accgtcgagg ccacgttgca ggaaaccgtg gatcaggtcc gggccgtggt
     8161 gggcgcgcac caggccgtgg tgagcctgac ggtctccgga gggcagacgc ggaccgtcaa
     8221 cgccgtgtcg tactcggaca aatatgccca ctgtcgcgac ctggtgcgat tgacccacct
     8281 ctatggcctg gatgcggccg tttgcgaaac caacaggccg atgcgcctga cccagtccgc
     8341 actggaggct gacccgcgct ggggcgggcc tggtgatgag gccgggacgg aaccgtccat
     8401 gcgaggctgg ctggccgttc cgctgaccgg ccagagtggc gagaacatcg ggctgctgca
     8461 attgagcgac aaatacgatg gcgagttcac attgcaggac cagtatgtgg gcgttgaact
     8521 cgcgcaactg gcatccatcg ccattgaaaa cacccagctt tttgagcagg tgcagcagct
     8581 caaccagggg ctggaagaaa aggtcgttga aaggactgcc gccctggcgc ggcaggaggc
     8641 cctgttccgg gcgttggccg agcaggcccc gcagatcatc tggaatgtcg acgttgacgg
     8701 caaggtgacc tatctgaatc gcaagtggta cgaattgatg ggtgggatac cggatgactg
     8761 gatgggatac aagtggtcaa gcgtggtgca ccccgacgat ctggccgaca tgtctgccaa
     8821 ctggaagata tcccgccaga gccggacgcc ctatgtgggg atgcggcgcc tgcgtacggc
     8881 ggacggcacc taccacacca tgtcttaccg ggcctctccc gtgcttgatg atgagggcag
     8941 cgtgctgttc tgggttggca tcgatgccga cgtgacggaa atcaaggaca ttgaaacagc
     9001 actgcgcctg tccaaccagg aactcgaagc cttttcctac tcggtctcgc atgacctgcg
     9061 ttcaccactg aacacggtgg acggcttcag ccgcctgctg gccaagcagc tcaatgccgg
     9121 cagcccgaac gaaaagatcc agcactacct gtcgcgcatc cacgcgggtg tggcccagat
     9181 ggggcggctg atcgaagacc tgttgtccct ggcgcaggtc tcgcgcatgc agttgcggca
     9241 tgaaacggtg gatttgagcg ccatggctgc ggaaattgcc ggtgaatgcc aggggcgcaa
     9301 cccggagcgc gtggccagca tccatatcga agagggtttg caggcccagg gtgatggacg
     9361 gttgatccac gtggtgatgg aaaacctgct cggcaacgcc tggaagtttt cttccgggcg
     9421 gtcacgtacc gatatccggg tcggacatac gactgacgct gccgggctgt ccgtgttttt
     9481 cgtgcaggac aacggtgcgg gctttgacat ggcctatgcc gacaagctct tcaatacttt
     9541 tcagcggctg catgccgtga ccgagtttcc gggaaccggt gtggggctgg ccaccgtgag
     9601 ccgcgtgatc ggccgccacg gcggcaaggt ctgggcgcac gccgagcccg gcaagggggc
     9661 caccttcttt tttaccctgc caagtggcgc ggccgccgct tgagcgcaat gcagatgtcc
     9721 gattgtaaaa aatgtatatg aattgatagc cgttaaattc aattgattaa atttattaaa
     9781 ttgtttaaat tgcaggtgta atacctaata ggcttcttta tgccccgcga accccaaact
     9841 ttcgaaaaag ccatcactgc gagcgatttt tccagcgatg actattgcgg aaccacttat
     9901 gccgccaaac tgctgggctt gtcggtcgcc accgtgcagt ccctggtcga gaagggcgag
     9961 attgaggcct ggaaaaccct gggaggccac cgccggctct ctctgcgtgc catcaatgcc
    10021 tatctccaga aaaacagtcc tcaacgtgcc caggtggacg cggatccacg caatcgcctg
    10081 agagttctgg tggtggaaga cgacgaggcc acctgcgagc tttacaaggc ccatttcgag
    10141 gaatgggaca tggcggtgga ctgcagcttc atgccctctg ccctggaggc cctgatggac
    10201 attgccagca tgcgcccgga cctcctgatc accgacctga acatgcccgg cgtggacggc
    10261 atggaaatgc tgcgcgccct caaacgcaac cagcagttgc tggccatgca gatcctcgtg
    10321 atcagcggct tgccgcggga atccatcacg gaacgaggcg gccttccacc caactcctac
    10381 ttccttgaga agccgatcaa tttcgactgg ctgcatgggt acgtaaccgc cctgctggtg
    10441 gctaaccgaa aatggcgcta gtgctccggc ccggacttgc cgacacatga cggcgcacct
    10501 tcgtttccct gcagtcgtcg cgaatccctg ccatggcacc ggttgtgcct atggttcgtg
    10561 cctcgccccg ggcgcgaacc gattgttttc atggggtttc gatgccgctg gggcgaagca
    10621 ctagggcggc ggtcgcctgc gtttgaagaa gcctggtctt ggcaccgctg acccttttca
    10681 aggcatggct gaagtcgctg aggggaagcc ggcgggcgct gcttccaggg gcccgcaccg
    10741 gacaggcgcg agccaacgca gaggccctgc aggccggcct cgacctgtcg tccgacggca
    10801 ttgtcctgct ggacaatgcc ggccgggtgg tggccttcaa cagttgtgcc caggcgctca
    10861 tgcattccgc actcggcaac ctccggggtg tggatttctg ggacgcggta caggaagaaa
    10921 ttgcagatga gtaccgcggc gccaccgagc aggcgttgct cgaaacggga agccacagtt
    10981 ttgtcgtctg gcacgcgttt gagaaccagt gggttgacta ctgcctgcgc cagcacgaca
    11041 acggcttgct ggtcacgctc agggatgtca gtgccgttca tcaggcgtca cgttcgctgc
    11101 gaaacagcga gtcctgcaac cagaccctgt tcgccgccca cccgcaggcg atgtggttgc
    11161 tcgataccgg ctcgcggcac ttgctcgcag tcaacaatgc ggcggcggcc ctgtatggcc
    11221 tggcgcctga agcgctgagg ggggcatttg ttgagtcgct ttttccggag ggggaggggg
    11281 catccctgct ggactccctg ccaccgagtg acttccagca ggaaatgcga ctgtgcaccc
    11341 agaagagggc agatggcgag ctcatgctgg ttgaactggc ctgctccagc gtgcaatggg
    11401 atctgcggcc ggccgtgctg gtcagtgtga ccgacgttgg tgcacgccac ctcgccgatg
    11461 cccatctgtc ccggctcaat gaagagctcg agatccgcat cgaacaatgc accggtgaac
    11521 tgcagcgcgg ccgtggcgaa ttcgatgctt tcacgagtgc catgttgcac gacctgaagg
    11581 cgccgctgca tgtcatcgac gggtttgcca ccacgctggc agagcgacat ggcacggcgc
    11641 tggatgcgca aggccagcat tacctggcgc gcatccaggc gagcagcctc cagctggcca
    11701 ggctgattga tgatttacgc accctgacct acttgcccag gatggtcatg gcgccggacc
    11761 gggtggatct ggcccccgtg tgcctgcgca ttgtggccga gctgcgcaaa cgggagccgg
    11821 cgcggcatct ggtgcttgaa atggaacctt cgctaccgct tgtctgtgac aaagaccagc
    11881 tgtccattgc gttggcctgc ctgctcgaca acgcctggaa gttcacctcc cgcaaagagc
    11941 agagctggat cagggtagga ctcatgccgg gggaaaattc cgcagacatc gtgctgtcgg
    12001 tctctgacaa cggtgccggt ttcgacaccg cctacaccga caaactgttc actgccttcg
    12061 agcgcctgca ctcgtcggtg gatttccccg gcactggcct gggcctggcg attgtcaagc
    12121 gtgtggcgca gctgcatggt ggtgaagtct gggccgtcgc tgctgacaag ggcggcgcca
    12181 gcttcttcat gtccctgccg caggagtcgg gctctgtcag cggctccccg ccggccgcct
    12241 gacccagtct ggcggcaacg cgcgccaact gaaagccgcc gcccgctgag ccgggcgccc
    12301 ctttcggggg cgcgtcaggc cggttcaaac cggtaaatgc cgtcgccgcc cagccggcgc
    12361 tgcagccggc cttcaaacca caaggtgtgc agatgcgcca cggcttcgcc catggcgaag
    12421 gtggtctggt gcagatccag ggcccgcttg aacatcacgg gcaggatgtc ggcgccgctg
    12481 caggctctgg cctggcaggc ttgcatgacc tcggccagac ggtcgtggtg atgctcgtgc
    12541 agctgcgcaa tccgtgtgtg gaggccccgg aacggtttgc cgtgcgaggg cagggttagc
    12601 gtatccgcgt gcaggggcgc gaacttgtcg atggactgca aaaacagggt cagcgggttg
    12661 gattcgggtt cggtgtcgta cacgctgaca ttggtcgaga tgcgcggcaa caccatgtcg
    12721 ccgctgatca gcgtgtgggt ttcggcgcag tgcagcgcaa tgtgttcggg cgcatggccg
    12781 tagccgctga tgcaggtcca gcggcgcccg cctatggtga tgacctggcc gtccagcatg
    12841 cggtggaagt gggcgggtac ctcgggcacc agcccggggt aataattggc gcgtgcccgg
    12901 atcagcgcca gggactcagg gtcggtcagc ccatgcgagg caaaaaaacc ggcggcactt
    12961 tcgccgccga aaccggtgct gctctgggtg gcgatgcggg ccgtgtggta atcggtggcg
    13021 ctgatccaca gcggcgcgtt ccagcgctgg cacagccagt gcgccaggcc gatgtggtcc
    13081 gggtgcatgt gcgtcacgat gacccgcaga attggcaagc cttccagttc gccggcaaaa
    13141 atctgttccc actgcgcctt ggactcgtcc ctgctgacgc agcagtccac caccgtccag
    13201 ccgggcacgc cgcccgcggg gccgggcagc tcgtcgcgca gcagccacag gttgatgtgg
    13261 tccagcgcaa agggcaggcg catgcgcagc cacttgacgc ccggggcgac ctccagggcg
    13321 ccgccggatg gcggcagggc atcgccgaaa gggtagtgca gctgggcttc ctgtggattc
    13381 atcttgtaag atcgccggtt ggattgacgt ttacgttaac gtaagcttga tccttccatt
    13441 acacccgaat tagctagcgg cttcacgagg gccctgtcac cagggtttca ccacatggca
    13501 accacgacca cgcactccat cagcgatctg gccaaagagt tcgacctgac cacacgcgcc
    13561 atccgttttt acgaagacat gggcctgttg cagccgcagc gcgaggggcc cggcgggcgc
    13621 aaccgcgtgt acagcgcgcg tgaccgcacc cggctcaagc tcaccctgcg cgccaagcgc
    13681 ctggggctga gcctgtccga ggccaaggaa atcatcgaca cctacgacag cccgcgcgac
    13741 accggcccgc aactgaagaa gttcctggcc gtgctggccc agcaccgcaa gcaactcgag
    13801 gaacagatgg ccgacctgca ggccaacctc gaagaggtcc ggctccatga gaaggaagcc
    13861 cgcgccttgc tgggcaagac ggccaaaacc gcaaaatagg ccataattga cgtttacgta
    13921 aacgtcaatc aggagccggc catggaactt ccccatttcg agcccaagga cgaggcttat
    13981 gcggcccgtg tgcaggacag ttttgcacgc cagggtgcca tggccaccct gggcgcctcg
    14041 ctggccggca tcacgccggg ccgggtggtc atcgagctcg attgggccgc cgggctgagc
    14101 cagcaacacg gttttctgca tgccggcatg gtggccacgg cgctcgactc ggcctgcggc
    14161 tacgccggct tcacgctgat gccgcttgac gccggcgtgc tgaccatcga atacaagatc
    14221 aatctgcttg cccctgccaa aggccagcgt tttcgcatgg tgggccaggt catcaaaccc
    14281 ggacgcaccg tgaccgtgac cgaggggcgg gcctatgcca tcgacgacgg ccgcgaaaaa
    14341 ctcattgcca ccatgggcgc cacgctgatg accatcaccg gccgtgacga cgtgaaaaac
    14401 tgaacaccac aaaaggagac cctcatgaac aacctgcccg gactcaactt ccagcttggc
    14461 gaagacattg acgcactgcg tgatacggtg cgcgaattcg cccaggccga aatcgcaccg
    14521 cgtgccgctg aaatcgacgc caacgaccag ttccccatgg acctctggca gaaaatgggc
    14581 agcctgggcg tgctcggcat caccgtcagc gaggagtacg gcggcgccaa catgggttac
    14641 ctggcgcaca tgatcgccat ggaagaaatc agccgggcca gcgcttccgt cggcctgtcc
    14701 tacggcgcgc acagcaacct gtgcgtcaac cagatcaagc gcaacggcac cgaagcgcag
    14761 cgcgccaaat acctgcccaa actgatcagc ggcgaacatg tcggtgcgct ggccatgagt
    14821 gagcccggcg ccggcagcga cgtcatcagc atgaaactga aagccgagga caagggcggt
    14881 tattacctgc tcaacggcag caagatgtgg atcaccaacg gccccgacgc cgacacgctg
    14941 gtggtctacg ccaagaccga acccgagctc ggcgcgcgtg gcgtcaccgc cttcctgatt
    15001 gaaaaagaca tgaagggctt ttcggtcgcg cagaaactcg acaagctggg catgcgcggt
    15061 tcgcacaccg gcgagctggt tttcaacaac gtcgaggtgc cggcgcagaa catcctgggc
    15121 ggcctcaaca gcggcgccaa ggtgctgatg agcgggctgg actacgaacg cgcggtgctc
    15181 accggcgggc cgctgggcat catgcagtcc gtcatggaca gcgtcattcc ctacatccac
    15241 gaccgcaagc agttcggcac cagcatcggc gagttccagt tgatccaggg caaggtggcc
    15301 gacatgtaca ccgtgctgca ggcgggccgc tcctttgcct acaccgtcgc caaaaacctc
    15361 gacctgctgg gcaccgagca tgtgcgccag gtgcgcaagg actgcgcttc cgtcattctc
    15421 tggtgcgccg agaaagccac ctggatggcc ggcgagggcg tgcagatttt cggcggcaac
    15481 ggctatatca acgactaccc gctgggccgc ctctggcgcg acgccaagct gtatgaaatc
    15541 ggcgccggca ccagcgagat ccgccgcatg ctgatcgggc gcgaactgtt tgccgagacc
    15601 atgtagagac caggtaaatt gcgacgcagg ccacaataac gcttatgacc tcttccatca
    15661 acgacctctt cacccacaac cgcgcctggg ccgcccagat ggagcgcgag cgtccgggct
    15721 ttttcacgag cctggtcaag cagcaaaagc ccaagtacat gtggataggc tgctccgaca
    15781 gccgtgtgcc ggccaaccag atcaccggcc tggagccggg cgaggtgttt gtgcaccgca
    15841 acgtggccaa catcgtggtg cactccgacc tcaatgcgct gtcggcgatc cagttcgcgg
    15901 tggaaatgct caaggtcgaa cacatcatgg tggtcggaca ctacggctgc ggcggtgtgc
    15961 aggcggcgct ggaaggcgcg cgcatcgggc tggccgacaa ctggatacgc cacatccagg
    16021 acgtgcgcca ccgccatcgc cagctgctcg acagcctggc cgaagaccgg cgcgccgacg
    16081 ccttgtgtga cctcaatgtc atcgagcagg tggtcaacgt ctgcgtcagc accgtcatgg
    16141 tcgatgcctg ggccaagggc cagaaggtca gcattcatgg ctgggccttc ggtgtcaacg
    16201 acgggctgct gcaggacctg agcatgacca tctccgaccc tgccgatctt gatgccttgt
    16261 accgtctggc ggtcgatcgc gtctgggact cccggcgccc cgcctgacgg ccatgtttcc
    16321 gcagcgcctc gactcgccgc ttgcctacga catcgccaag gcgatgatgg acggcttcaa
    16381 ccggcactac cagctgttcc gtaccgagtc ggcgcgcgcc aaacaccgtt ttgaaaccgc
    16441 cgactggcac ggccagcagc ggtcgcagcg cgagcgcatc gagttctacg acctgcgcgt
    16501 gcgcgaggcc tcgacccggc tggagcgcga gt
//
LOCUS       DAIDFG010000032        16470 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_32_length_16470_cov_18.237588, whole genome shotgun sequence.
ACCESSION   DAIDFG010000032 DAIDFG010000000
VERSION     DAIDFG010000032.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 16470)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16470)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16470
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32_length_16470_cov_18.237588"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    8567..8666
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3102 a         4836 c         5205 g         3227 t
ORIGIN      
        1 gcttccattg gttccggttc caggtgttgc tggggtggtg tcgagtcgcc gcagaacgat
       61 ggcagtcgtg ccggccttgt gattgctcag gcagctcagc gcagctgttt ctgttgcggt
      121 gaaacctgtc agcgcaaccg gtgcggtcag ggccgcgttg ttccacccca gatcccccag
      181 ggctgttgcg caggctggag ccgtccccag ggagatgccc gttgtggaaa cttcgccgta
      241 gtacccggcc acgctgacgt cttcctcgat cagttcgaga gcgtagcggc cattttcgat
      301 ctggcggata ctcctgtcga tttcctggcc ggtctggctg ctattggcaa acagcatggc
      361 caaccctgcg atcaggatca agccgatggc gatagacacc attagttcaa ccagggaaaa
      421 ccccttggtt gattttcctg agcggtaacc ggaataacgc ttcatcgcgg tcatgatgca
      481 agtgtggcga tctgtacaac ggtggtcact gtcctgcgca ggttctcgct ggaataggcg
      541 tcctggccgc aagcgttggg aggtgcaccg gttgcctgaa caccttgcca cgcaatgctg
      601 atcagatact ggttggccgc aggactggta atgcagcccc gcgcgcccgt catggccccg
      661 agcttggctg tgccctggac ttcggccgca ccctgcaacg acaggctcca ttcgcagaga
      721 tccctggcag cacggtcggc cagggtggtg cagtcgccgg gatcactggt gccgatgttg
      781 ttgcccacgt aggctgcggc gttcgcgcga ttcaaggcga tacgttcgga gatgtcgctg
      841 atcagaatca gcgcctgcga ccgctggtag ccctcgagtt gggacagggc cgtctgtgcc
      901 tgcaggccga caaagccgag gacgccaaag gcaatgatca acatggcaat gagcagctcc
      961 atcattgccg caccttgctg gttttgggat ttcatcatgc cgggcactcc gtggtcgcac
     1021 ttttcggccg cccggacgga tccagcgaga tgcatcgcca gcgggtactt ccgtcagaca
     1081 agccaaagcg cacgactgtg tcggaggtgc ttctgccgtt gcggtcaaag gtgatcgtgg
     1141 tgggagcggt ggtaaagacc accccgctcc ctacgccgtt ttgcgcgctg agaacttcgg
     1201 cgctggacgc gaccttgaca ttccagccgc cggcccagtc ggcagttgaa ggagtgagcg
     1261 tgacgctttc ccctcgcttg acggcctcgc ttcttgccag aaccaggtca ctgactatgc
     1321 tgtaggccgt ggcccgcacc cgctgcgctg ccaggagctg ccggaacgag gggcttgcca
     1381 gcgatgcaag gacagccaga atggccactg ccaccatgag ttcaatgagc gtaaaacctg
     1441 cattgatccg cgttttcgca agtttcatga tggggtctca ccagcaagtg gtgggggatt
     1501 ttgtacctgt ctgggtgatg ctcaggatgc cgcatttgtc tgccgcctgg gctcccgtcg
     1561 gtgtggctgt tgccgtgaag gtcggcacgg ttgccgtccc taccgtcaag gaaatcgtgt
     1621 aggtctgctg gacggtggct gggatggaaa cattcagggc cgtggtgtcg gcggcatagc
     1681 tgcgtgtgtc cagcagcatt tgctgctgtc gcgcgctgat gttcagcaag gcggcctgag
     1741 cttctgcacg ccttgcttta cgaacctggt caaggtaggc gggataagcg acggccgcca
     1801 ggatgccaat gacggcaacc gtgatcatta gttcaatgag ggtgaaccct cggctaggtg
     1861 ccatcttaat caaaactcca taattgtgaa atttgataaa ttaaaaatat tcgccaaatt
     1921 cgatagaaat aggttcttat tagaggcgaa aatttcctaa taggtagcac attgatgcaa
     1981 actaaagttt aaagcaatat gtcacagagt gatgtttaca aaaagttaca aatatgaaaa
     2041 tatcggacaa ataacgccta aaaaacaggc aaaaaatggc tgaatgtaac tatgtgtgtc
     2101 ttttgcatgt gttcactgca agttttgctg aaaggtgatg cgcggccaga agtaacggct
     2161 ctggcgcctg cacgagcact gctaaaaaag ggactacccc gatatcaatc cggcataagt
     2221 ggcggaaact tgcaggcatg gatggcgtgt tttgttcgag gatgcgcttt tagaaacaag
     2281 gacaaaagac aggacatgaa gcacagaccg ccgccactgc ggtggcagtt tttttgcgaa
     2341 gttccgcact gcggtcttct gctcccgcgt gccgttctcc cgaggccggg gcattgaagg
     2401 tactcgagtg aagcacaacg aagacctcgc cgaagaaaac cgtcgcctga aggcgcggct
     2461 gcttgaactt gagagcgcca acgaccagct cgatcggatg cttgccgaac aaaagcgcga
     2521 ggccgatttg ctcaggcgca gcgcgcaggg attgcgcgac ctggccacga tatccgcgga
     2581 ctggtactgg gaacaggatg tagagcatcg tttcgtcagg ttttccgggg agcaggcggc
     2641 caccgagctc gatatcgaac gggacagcaa catcggcagg cgccggtggg agctggtcgg
     2701 tgcggtgccc tttaacgggt cctgggaggc gcaccgcgcc gtgctggatg cgcatcagcc
     2761 gtttcgcaat ttcgaataca tgcgcaagct cggcgacgac ttgccgcact acctgtcggt
     2821 cagtggcatc cccgttttcg acgaccagaa ccgcttcctc ggttatcggg ggacggtgca
     2881 tgacatcagc gcgatcaaac gctcggaaga agccgagcgg caggccgcga gcttcctcga
     2941 tgaagtggtt gacaacctgc cgattgccct gcaactgaag tccgtgcagg acgggttcag
     3001 gattgtgggc tggaacaagg ccgccgcggc cctgtacggg gtgacccgcg aagaggcgat
     3061 gggccgcacg gtgcacgatc tctggccgaa ggaggacgca gaccgcatgc acgccgccga
     3121 cctcgagctg gttgcagcag gcgtcatgca ggacctgccc gaccggctga ccgtgacaaa
     3181 acaccgcggc gccatccgcg ttcacatgcg caaggttccg ctgaaagatg ccagcggcac
     3241 ggtcacccat atgctggtca ccagcgggga tgtcaccgga cggctggcgg ccgaggcccg
     3301 cctgcgcgac agccaggaac gctttcgcag cctgacccag ctttcctccg actggtactg
     3361 ggaactggac gaccagttcc gcttcacccg gatgtccagc ggcgcggccc gttttggccg
     3421 gcttcccgcc ggcccgggag gacaggtcct gatcagcgaa tacatcggcc ggacgcgttg
     3481 ggacatcgac gataacccac gcaacaaggc ggcctgggcg gtgcatcgcg cgcagctgga
     3541 aaaccacgaa actttccgcg acttcgaata cgagcgcgtg gacctggacg gcacgcgcat
     3601 ctttttctcc atcagtggcg agcccgtgtt tgacatggac gggaaattca cgggttaccg
     3661 cggcgtgggc accgacatca ccgcgcgcaa acagactgaa gcggcgctgg tagccagtga
     3721 agcacgcttt cgcagcgtgg tgggcgcgct ggccgaaggt gtcatcatgc gggatgccga
     3781 ggggcggatc ctcgactgca atgccagtgc cgaacgcatt cttggccaga ccctggccca
     3841 gatgcgaggc cagacccttg tcgggccgga gtggcagacg ctgcgcgaag acggttcgct
     3901 gatggccgag gatgaacggc ccagtgcggt ggccaggcgc aagggcctgc cgcagtcaaa
     3961 cgtggtggtc tgttaccgca agcccgacgg cagggatttg tgggtactga tcaatgtgag
     4021 gccgttgttt gacaatgccc gcgacgcccc gtccgggttt gtcagcactt tcaccgacat
     4081 caccgagcgc aagcgcgccg agatggaaat tgtccggctc aacgtcgacc tggaaaaccg
     4141 ggtctcgcgc aggaccgccc agctggaggc ggccaacacg gagctggagg ctttctccta
     4201 ttccgtggcg cacgacctgc gttccccgct gagttccatc gacggctact gtgccttgct
     4261 gcaaaaggcg gtgtcgccgc aggccgacga acgtgcgaac cattacctga gccggatacg
     4321 cggcggtgtg cggcgcatgg gcgaactcac cgacgggttg ctgctgcttg ccaggctgtc
     4381 gcgcaccagc ctgaactggg agactgtgga cctcggtgcc gaggcagccc gggttgtcgc
     4441 acaattgtcg gagaccgagc ccggtcgcga ggttcaggtg accgtcgagc ccggcatgct
     4501 ggcccgcgcg gacgtctcgc tgatccggca ggtgcttgag aacctgatca gtaacgcctg
     4561 gaagttcagt gcgaaaaaga cccgcacgga gatcatgatc ggcagggaaa ccgttgccga
     4621 ccagccgccg gtgtattttg tccgggacaa cggcgcggga ttcgacatgg cttatgccga
     4681 caagctgttc ggcactttcc agcgcctgca ttcgccggaa gagtttgctg gcagcggcat
     4741 cggcctggcc acggtcaaac gcatcatcat tcgtcacggc gggcggatct gggcaagttc
     4801 cgtgttgggc gaaggcagca cgttttattt cacgctcgga agcgaccagg gcaatgcctt
     4861 gccgggtgac gatgtttccg gagaagacgc gggcatcgcg ctggccctgc cccgctcacg
     4921 ccacctgttc agggacaccg gcccggccgc ccgggatacg gcgggttcgc tgatcagcag
     4981 tgacaacgat attttttccg ccggcgacca gcagttcagc agtgctttcg agcatgcagc
     5041 gatcggcatg gcgctgatcg ccatcaactc ccgccggatc cgggtcaaca gtgccttttg
     5101 ccagatgctc ggctactccg aagcggagat gctggcccgc accgtgcacg acctcaccca
     5161 tccggacgac atcgaatggg atctgctgca gcgaaaaagg gcgctggacg gcgagattga
     5221 aacctaccag cgggaaaagc gctatatcca caagtccgga catattgtct ggggctctct
     5281 gacgtgttcg ctggtgcgcg accctgaccg caagccgctg cacttcattg tccaggtgca
     5341 ggacaccacc gaacgaaaag aggccgaacg catcctgcgc gagagtgaag agcgctttcg
     5401 cgcactgacc gagctgtcgt ccgactggtt ctggcagcag gatgaaaatt tccgttttgt
     5461 tccggttcca ggcgaggcga cccggctgtc ggcaatttcc cgcgacgcca tcggcaagac
     5521 gccctgggag ctgaaccatg tggacatgga tgaccgtgtc tgggagggcc acaaggcaca
     5581 gctcgcgcgg cacgaggttt ttcgcgacct ggaaatttcc ctgcttggcc gcaagggaag
     5641 tgttcgtcat atcagcatca gcggggagcc gatcttcgat tcgtccggtc gttttaccgg
     5701 gtaccacgga accggccgcg acaccaccga gatgcaccgc gtgagtaacg cgctgcggga
     5761 cagcgagcag cagttgcgcg agatcacgga caccgtgccg gccctgatcg cctatgtcga
     5821 caaggcgcag cgctttcgct ttcacaaccg ggcctatgaa gaagggtttg gcctgtcgca
     5881 tgagcagatc gacggaaaaa ccctgctcga ggtcatgggc gaggagcttt accaggtcgt
     5941 gcgcgcccag gtagacgagg tcctgtcggg ttacccggtg gtctacgagc gcacccagaa
     6001 aaccgcacgc ggcgaccacc gcgactacgt ggtcaattat tttccacgtt acggcgacgg
     6061 caaggaagaa ggcgaggtca tcgggtttta ttcgctggcg accgatgtga ccgaactcaa
     6121 gcgcatcgac cgcatgaaga gcgaatttgt ctccacggtc agtcacgagt tgcgcacgcc
     6181 gctcacctcc atccgtggtt cgctggggct gatctccggc ggcgtggccg gcgagttgcc
     6241 ggagcgcgtc aagaccctgg tggacattgc caggaacaac tgcgagcgac tgatccgtct
     6301 gatcaacgac atcctcgaca tcgaaaagat cgagtccggc aagatgcgcc tggacctgca
     6361 ggtggtcgac ctccagccct tgctggtaca ggtgctggcg gccaacgaag gttttggggc
     6421 tgcgcagaac gtcagcttgc gcctgaattt tccggagggt gacctgcggg ttcatgtgga
     6481 cactgaccgc ctggcccagg tcgtgaccaa cctgctgtcc aacgcgatga aatattcacc
     6541 gccgggcggg gtggtggagg tcgatgtcaa acgcgccggc ctcggcgtgc gggtggaagt
     6601 gcgtgatcat gggccaggcg ttccggaaga attccgcaag cgcatcttcc agaagttttc
     6661 ccaggcggat tcgtccgaca cgcgccagaa gggcggcacc gggctgggcc tgaatatttc
     6721 cagggcgatt gtcgaacgcc tcggcggcag cgtgggtttt gacagcaaag tcggcgtggg
     6781 ctccacgttc ttctttgaac tgcccgaatg gaaagaggct gcaccgcgcc ttcccgtaga
     6841 aacccgggtt ttcacccggc catgcgtgct gatctgcgaa gacgatagag acattgcccg
     6901 cctgatcgcg atgatgctgg acaagggcgg ctttgacagc gacatggttt acagcgcctc
     6961 cgaggcgatg gcccacctgg cggccaaccg gtatgccgcg atgacgctgg acctcaagct
     7021 gcccgaccag gatggcatca cgctcatccg caccctgcgc agccaggaag gcacgcggga
     7081 tctgcccatt gtggtggtgt cggccatggc cggggagggc cagatccagt tcaacaacca
     7141 gtcgctgacg gtttccgact ggctcgaaaa gccgattgat gaaaatctgc tgatccgcag
     7201 tctgcgccat gcgatgaagg gcatggccga gggtaagccc cgcatcctgc atgttgaaga
     7261 cgatctggat atccagcgca tcacggcagc gatcgtgcag gacttcgcca cctttgaatt
     7321 tgccgccacg ctgcaggaag cccgcgcatg cctggcggcg cggacctacg acctgatcct
     7381 gctcgatctg agcctgcccg gtgaatcagg ctgggatctg ctggccgaca tcgaagcgct
     7441 ggagcggccg ccgccggtcg tggttttctc ggccagcgag gtggaccgcg cccagggaac
     7501 ccgggccgct gccgtgctgg tcaaggcgca aacctcgaac gatgagttgc tgcagacgct
     7561 gcagcgggtt ctcagccaga cgcgctgatc gaagcaaggg tgccccgcct ttgagcggcg
     7621 ggcctgctgc cgcaagcccg cctcagggtg tttacatccg gcccaggtcc tgaatggcgt
     7681 ccaccaccat gctggttccc acggcgatca gcagaatgtc gctggccacg cgcacatatt
     7741 tgtggccggc agggggcgtg cccagctgga tcagcacggc gttgggcacc gggtaaaaca
     7801 ccacgtcacg cggcagcggc tggcccatgg cccatttttt ggcctgcccc ggaggcatgc
     7861 agccattgtt tttcttggcc aggccgggag ggcagcgacc tgccttgtac tgcttgccgt
     7921 agtagtcgcg gatcaccacg cgctgctggt cgccgaagta agcgccgacc cggacgtcct
     7981 gttgcgccgg gcgggcctgc tggccgccct tgttgttgcc gttgcccttg tcgccgcgtt
     8041 cggctttgtc cggtttgccg ccggaacgtt cgttttgctc ctgctttccg cctttgccct
     8101 ggccggccca ttcgggtttc tgggcccagg tggagcctgc cgcgaacgca aacgccaaaa
     8161 cgaggggcag cgcgcgaagt tgatgcagtt gtttcgtgtt catgatgtga ccttggtagc
     8221 tagctaatcg gccgggcctg ggtccggtgc ttggttgaat actttataac gcgtcaccct
     8281 tgattttgtt gtccgatttg gctgataggg ggccttccgg cgatgttgtc ctacagccag
     8341 gcgcccgtcg cgcgaggcgt gccgagtcgc tcaccagggt tgaaagccag acccagccgg
     8401 agaagctggc cagtgctgga cgcggagcat ggcccaacag acgttgcagc gcggcgcaca
     8461 ggctgctgaa gtcatcgcac ttgccgattg cgagcgccga tgcgcagatg ccggaggaca
     8521 cagcacggga tgccaaaaag ggctgtagcc caatacggga aagccgnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnagga cacagaacgg gatgccaaaa agggctgtag
     8701 cccaatacgg gaaagccgaa ggcgctatca atcagggagc gttcccgccg tgattttcca
     8761 gtcagcggct tttggcctga ttcaacgcgc tttcgaactc ctggtgcgac agggtgcggt
     8821 ccttgttcgt gtcggcttca tcaaagtgcc tggccacgcc gcgaaagccg gaggcctctt
     8881 ctcggctgat cttgccgtcc ttgttggtgt ccatgaaact gaatgcccgc gcgatgtcac
     8941 cggtggtgta tctgggttcg gtagaggccg atgccgtgga gactaccgag gtggacggcg
     9001 caggctgtgc ctgaaccgct ggcgacgtgg ctggcgctgc cgcggccggc tgggcgtgtg
     9061 cggccggcgc cagcagggct gccgctgcag tggcgagggc gagggcgagg gcgaggaggg
     9121 tccgggtcgg ggtacgagat gcgccccgtg aagcggtggt caaagtcatg ggtttcctga
     9181 cggtagtggc aaacatgagc accattgaac gccaatagac gtgccggcgc cagcaaggtt
     9241 gcacggtgtt gcgtgacttt acatccgtgg aggatcggga cacatttctt tgcgctcacc
     9301 aggctgttcg ggctgcccgc tgacagcaac tgtccggcct gtcccacaag ttgcaacccc
     9361 ctgggctggc gagcagcccc atcggtggtg cgatcatcgg gaagcgctac cgcgcaaaca
     9421 aaaaaccggt cttcccggtg gcgtctgtgc ggtgggcgat acccccatcc agcaggaacc
     9481 gcgaagccat gaacctcccc gacgaagcga catccaccgc gcttgaagac aagaccttcc
     9541 tgcttgtgct gctggccgtt tcggtggcct tcctgtggat actctggccg ttttacgggg
     9601 cggtgttctg ggctgcggtg ctggccatca tgttcaagcc gctgtacctg cgcgtgctca
     9661 gggccatgcc ggccaggcgc gtcggcgccg cgttgctcac actgctggtc atcctggtgg
     9721 cggtcatttt gccggtgggc attgtgaccg cgctgctgat ccaggaaggc ggtggcgtct
     9781 accagcgcct gcagtcgggt gaactcagtg tggcgcgtta cttccagcag gtctacgagg
     9841 ccctgccggc ctggatcacc agtctgctcg atcgcttcgg catgaacaac ttcgggctgg
     9901 tgcagcaacg gatcacggaa gggctgacca agggcagcca gttttttgcc acgcaggcct
     9961 tgagtatcgg ccagggcact ttcgactttt tcgtcagttt ttttgtcatg ctctacctgc
    10021 tgtttttcct gctgctcgac ggttcggccc tggtcatgcg catcaaggat gccgtgccgc
    10081 tcaatgcgga ggtcaagcgc aagctgtcag ccaagttcac cacggtcatt cgcgccacgg
    10141 tcaagggcaa catcgtggtg gccctgatcc agggcgcgct gggcggtctg atcctgtggg
    10201 tgctgggtgt gcatgcaccg gtgctgtggg gcacgctgat ggcctttttg tcgttgctgc
    10261 cggctgtcgg ggccgccatg gtgtggctgc ccgtcgcgat ttatttcctg gccacggggg
    10321 ccatcgtcca gggcgtggtg ctgatcgcct tcggcgtgct ggtcatcggc ctggtggaca
    10381 acatcctgcg cccggtgctg gtcggcaagg acaccaagat gcccgactac gtggtgctgg
    10441 tgtccacgct gggcggtatg gcgctgttcg gcctgaacgg ctttgtcatc ggccccgtga
    10501 tcgcggcgat gttcatggcc gcctggggtc tgttttccaa ggcgcggcag gcgcaggtgg
    10561 ccgagccgcc caagcggccg tgacgtctgc gtaacggcat ctcctggtgg accagtttcg
    10621 gagtcaggtg tcgggaagtg ccggacccac cctcaacggg gaaccacctt gtaggatcgg
    10681 ccatccgctg ctgagcccga gctttctccc tgaacaatca gcggtgcaaa cacgcgaatt
    10741 tccgtggtgc ccgggacggt gaattgggtt tccagcgtgg actttccact tcccttggtg
    10801 atcaccttga catcctggcc aagctccgaa ctgtcggccg cgagaacgag aagtttgatt
    10861 gttcccgatg gcgaggtcag gacatgactg acgtcaaccc tcagcctgac ttgttcgccg
    10921 gcgcgaagaa ccctggtggt gtcgggcgag atggacgtga tttgcgccat cgagccgaag
    10981 ggtggcatgt tggcgggttg ccccgcagat tggcagccga ccaaaaagcc ggaaacggcc
    11041 atggccgcga acagggaggg tgaaaatttg atgagcatca cgccatcctg aaccggatga
    11101 agataaatgt caaaggaggg tgtgtgactg gcttggcagc gagcaagcgc tcgttgtgat
    11161 cctcttgcgc atacgccacc cggcgtattc cccaatcccc tgccggtgcc cagaatcgcc
    11221 tcatcgcttc gcaaaagtcc cttccctggt ggctgtgcga ggcgaaacgc gaactactta
    11281 cctaccggag aagcacacat gcaacgtcgt tttaccctca aggcgctgag cgccgccgtc
    11341 gccctggcgg gcctctctgt cctgccagcc catgcccagt cgggcggcac catcaaggtg
    11401 ggcattctgc acagcctgtc gggcaccatg gccatttcgg aaaccgtgtt gaaagacacc
    11461 gtgctgatgg cgattgacga aatcaacgcc aagggcggcg tgatgggcaa gaagctcgag
    11521 cccgtgatcg tggaccctgc ctccaactgg cccctgtttg ctgaaaaaac caagcagttg
    11581 ctcggccagg acaaggtttc ggtgattttt ggctgctgga cctcggtgtc gcgcaagtcg
    11641 gtactcccgg tggttgaaga aatgaacggc ctgctgttct accccgtgca gtacgaaggt
    11701 gaagagctgt ccaagaacgt gttctacacc ggtgcagcac ccaaccagca agccattcct
    11761 gccgtggact acctgatgag caaggccggc ggcggcgcca agcgctgggt gctgctgggc
    11821 accgactatg tgtacccacg caccaccaac aagatcctgc gcgcctacct gaagagcaag
    11881 ggtgtgaagg acagcgacat cgatgagaaa tacacaccgt tcggccattc cgactaccag
    11941 accatcgtgg ctgacgtgaa gaaattctct gctggcggca aaaccgctgt ggtctccacc
    12001 atcaatggcg actccaacgt tcctttctac aaggaactgg gcaacgccgg cctgaaggcc
    12061 aaggatgtgc cagttgtcgc tttctccgtg ggtgaagaag agctgcgcgg cgtggacacg
    12121 aaaccgctgg tcggccacct ggctgcctgg aactacttcc agtccatcaa gagcccggcc
    12181 aacaccgagt tcatcaagaa atggtcgacc tacgcgaagg ccaagggtat cgccggccac
    12241 aaggacaagc cgctgaccaa cgatccgatg gaagccacct acatcggcat caacatgtgg
    12301 aagcaggcgg tcgagaaagc caagtccacc gacaccgaca aggtgattgc cgccatggct
    12361 ggccagactt tcaaggcacc gtcgggcatc gtctccaaga tggacgagaa aaaccaccac
    12421 ctccacaagt cggtgtttat cggcgagatc aaggccgacg gccagttcaa cgtggtgtgg
    12481 aagacacctg gcccggtgaa agccaagcca tggagcccgt acatcgaagg caacgacaag
    12541 aagcctgacg agccggcaaa aatggccagc aaataaggcc gctgccgcag gtctgtcgac
    12601 ctgcggtgca agccgcacct gggttgcaga aacaggtgcg gctttttttg gtctggccca
    12661 attcttgcac tactccggtg catgaatcgg attatttttt tccaaggaag ttgcagtctg
    12721 tgcaccgcag cgagccgcac cggcgcgcta ccgtgcccac cctacaggtt ctttaccatg
    12781 atgatgcgca cacttctttt atttgccgct tttttactgt ccgccggtgc acacgccttg
    12841 acgccggaag aggtcagggg catggcgctg ggtgagaccg aagcccgggt cgaggcgctg
    12901 gtcaaggcct cggccgtgcc tgacgaaaaa actgctgcct ttatccaggc cctggccgac
    12961 gacgcggtga agacggccgg cgacaaggtg tttgtcatca aggacgacaa gggctacgac
    13021 cctgtcagcg gtgccgagtt gccggtgcct gccgaggccg aggacgtgat caacaacaac
    13081 ctgatgcgtg gcgagctgga caatgcgctg gcctcactca aattattctc caaggatgaa
    13141 aaggtccgcg ccgaggcgat caagacgctg tctggcggtg ccgatgaagt ccgcctgccg
    13201 ctgatcgaga aagcgtacgc ggccgagacg gttcctgcgc tcaagaccca gttagagatg
    13261 gtgcgcgcca ccatcctgct gggtagcagc gacaagcgca aacgcctgga agccgcggcc
    13321 cgcctgtcgg gcagcaacaa cccgatcagc aagacggtgc tgatcgcacg cctggcggaa
    13381 gaggacgacg ccgaggtcaa gagcgcgctg caaggctcgc tgcgcaaggt cgagtcggcg
    13441 ctggcctggg gcgacaagct cggtgccatc ttcagcggga tcagtctggg cagcatcctg
    13501 ctgctggtgg cgctgggcct ggccatcacc tacggcctga tgggcgtcat caacatggcg
    13561 cacggcgaac tcatgatgat tggcgcctac gccacctacg tggtgcaggg cctgttccag
    13621 aagtatctgc ccggcctgtt cgactggtac ctgctggcgg ccgtgccggt ggctttcatg
    13681 gcctcggcgc tgatgggtgc ggcattggaa cgcggcgtga tccgttttct ttacggtcgc
    13741 ccactcgaga cgctgctggc cacctggggt atcagcctga tgctgcagca actggtgcgt
    13801 tccatcttcg gcgcacaaaa cgtcggcgtg gagaacccgg tctggatgag cggcggtgtg
    13861 caggtgctgg gcaatctgtc gctgccttac aaccgcctgg tcatcatcgg ctttgcgatt
    13921 gcggtgctgc tgggcatggc ctggctgatc agcaggacgc ggctgggcct gtttgtgcgc
    13981 ggcgtcacgc agaacaggcc gattgcttcc tgcatgggtg tgaacaccgc gcgggtggac
    14041 acctatgcgt ttgcactcgg ctcaggcatt gcgggcctgg ccggctgcgc gttgagccag
    14101 gtcggcaacg tcggcccgga cctgggccag ggctacatcg tcgatgcctt catggtggta
    14161 gtgctgggcg gtgtgggcca gctggccggc accgtctatg cggccatggg cctgggtatc
    14221 ctgaacaaat tcctcgaagg ctggaccggt gcggtgctgg ccaagattgc ggtgctggtg
    14281 ctcatcatca tcttcattca aaaacgcccg caaggcattt ttgcgatgaa gggccgctcc
    14341 gctgaagcgt aaatatgagc ctgcccatga cctcctctct cgttctacct gccaaaggcc
    14401 ccttgctcac gcgcgccggc tggagcgcct tcatgctgtc gctgctgctg gtctgcgcgc
    14461 tggcgcctgt gctgaacttg ctggtgccgg agagcagcat gttccacatg agcacctacg
    14521 cggtggcgct gctcggcaag atcatgtgtt acgccatcgt ggcgctggcc atggatctga
    14581 tctggggcta taccggcatc ctgtcgctcg ggcatggcct gttcttcgcg ctgggaggtt
    14641 acgccatggg catgtacctg atgcgccaga tcggccgcga cggcaactac aaaagcgact
    14701 tgcccgactt catggtattt ctcgactgga aggagttgcc ctggcattgg tccttcagcg
    14761 acagtttcgt cgccacgctg ttcctgatcg tgctggtgcc cgggctggtc gcctttgtgt
    14821 ttggctactt cgctttccgt tcacgcatca agggcgtgta tttttccatc atcacccagg
    14881 ccatgacctt tgcggccatg ctgctgttct tccgcaatga aaccggtttc ggcggcaaca
    14941 acggtttcac cgacttcaag cgcatcctcg atacgccgat tgccacgccc accatgcgca
    15001 tggggctgtt cgtcatcacc ggcctggccc tgctcggctt ctttctgttt gccaagtggc
    15061 tggtgcgcag caagttcggg cgcgtgttgc aggcgattcg cgacgccgag acccgcgtga
    15121 tgttctcggg ctacaacccg atcggctaca agctgaccat ctggaccctg tcggccgtga
    15181 tgtgcggcat tgccggcgcg ctgtacgtgc cgcaggtcgg catcatcaac ccgagcgaga
    15241 tgagcccggc caactcgatc gaaatggcga tctgggccgc ggtcggcggg cgcgccaccc
    15301 tgatcggccc catcctcggc gccttcatcg tcaacggcgc caagagctgg ctgacggtgg
    15361 cctaccccga gtactggctg tatttcctgg gcatgctgtt cattgcagtc acgctgttcc
    15421 tgccgaacgg tgtggttggg ttgatcaaga aactccgcaa agggggtgag aaatgacgcc
    15481 cgatcttctg gagcagggtg ccgcacgtat tgaaaaacgc cgcgcggccc tggaggcgag
    15541 caagtccaac accgaatcgg gcgggcgcaa cgccagcaca ggccgcgttg ccacgcccgg
    15601 cgaggtggac gtgacacacg gccgcatttt gtacctcgaa gacgtgagcg tgagttttga
    15661 cggtttcaag gccatcaaca aactctcgct cgacattgca ccgggcgagc tgcgctgcat
    15721 catcggtccc aacggcgccg gcaagaccac gatgatggac atcatcaccg gcaagacccg
    15781 gcccgacgaa ggcacggtgt ttttcgggag cacgattgat ttgctgcgcc acaccgagcc
    15841 gcagatcgcg cagctcggca ttggccgcaa gttccagaag cccacggtgt tcgagcaact
    15901 cacggtgttc gagaacctcg aactcgcact caagaccaac aagggcgtga aatcgtcggt
    15961 gtttttcaag ctcgactccg cgcaaagcga ccgcctggcc gaggtcctgg tcacgataca
    16021 cctggccgac agcgtgggcc gcatggctgg caacctgagc catgggcaaa agcagtggct
    16081 ggagatcggc atgctgctga tgcaggaccc gaagcttctg ttgctcgacg agccggtggc
    16141 cggcatgacg gacgaggaaa ccgcccgcac cgctgaactg tttctcacgc tcaagggcaa
    16201 acattcgctg atggtggtgg agcacgacat gagtttcatc aagacgattt ccgagatcgt
    16261 cacggtgttg tgtgatggct ctgtgctggc gcagggcacg ctggaccagg tgcaggcgga
    16321 tgaacgggtg attgaggtgt accttgggcg ctgatcctcg gttcgttcag acggagcctg
    16381 tcggagccgt ctttgcctcg tgcattggag gttcgccagc aagccggggg ttgcccggcg
    16441 gcaactcact tttcttttgc gtcgccaaaa
//
LOCUS       DAIDFG010000033        16192 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_33_length_16192_cov_18.718659, whole genome shotgun sequence.
ACCESSION   DAIDFG010000033 DAIDFG010000000
VERSION     DAIDFG010000033.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 16192)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16192)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16192
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33_length_16192_cov_18.718659"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2866 a         5486 c         5223 g         2617 t
ORIGIN      
        1 atggcgctga tcaatgccgc gcagaaaaca ctggacatcc agtactacgc gatccatgcc
       61 gacgagacca cgctgcggat gtttgacgcc ctgcgcgccg ccgccgcccg cggcgtgcgc
      121 atccgcatcc tgctcgacga cttcaacacc gccggcaaaa acgcccaggt gctcaaactc
      181 gcctttggga aaaacatcga gctgcgcctg ttcaatcccc tgcccggcgg gcgcgggtcg
      241 ctgtttttcc gcatcctcgg ggcgctgaaa gacatggacc gcctccagcg gcgcatgcac
      301 aacaagattt ttgtcgccga caacgcggtc gccatcaccg gcgggcgcaa cctcggcgaa
      361 acctacttcg gccagggcga gtccaccaac ttcatcgacg ccgacgtgct ggcggccggg
      421 cggatcgtgc gcgaactctc gcgcagcttt gacctgtact ggaacaaccc gctggcctac
      481 ccggtgcagt ccctgatgac cgcagaacag ctggaagcca tgaaaccgcc gccggcagcg
      541 ggctcgccgg caggccaggc cgggccggtc accagcgaag ccagcgcctc cgccggcggc
      601 aatgccaccg gcgccgcccg ggccccaacc gcgaaatccg ccgtgcgcaa ggtcaccgcg
      661 ctggacggct cggtcagcgt ggtcgaaccc ctgcccgaca gcaccaacct ggcgctgctc
      721 gaatggatct gggcgccgtc aacgctgctg gtcgacaagg cttccaagat cgccgccgac
      781 gccgacgcgg tggaagcgtc gcaggacacc acggtcgatg gcctgctgca actgatggca
      841 gaggccaaaa gcgacctgct gatcgtctcg ccctactttg taccgggccg gcgcatgatg
      901 gaccagctcg cggccatccg gcgcaaggac gtgcgcatcc gggtgctgac caactccctg
      961 gcgtccaacg atgcgcctgc cgcccatgtg gggtacgccc gttaccgcaa ggaactgctg
     1021 ggcctgggca ttgaactgta tgaaatgcgc gccgagcagg aaggcacgct gagcggcttc
     1081 agctctctcg gcagtaccgg ctcggcaggc ggctcacgcg ccagtctgca tgccaagatc
     1141 gtggtgctcg acgataccct gctggtgatc gggtccatga atctggacct gcgctcccag
     1201 ttgcagaaca gcgaggtcgc gctggtgatc cgcagccgca aactctcggc cgacaccacc
     1261 cggctgattg aaccggcgct cgccaagggc gcctacaagg tcgagctgga aaacgaccag
     1321 ctgatctggc gcgcgcctca aggctcgcag ctgaaggact cccgcacgga gccggacgcg
     1381 agcctggggc tcaagctcat gctcatgctg attggcccgc tggcgcctga cgagatgctc
     1441 tgagcggcca cgcggctttt tcgggggcac ggacggccgg tcgccaggcg gcaagcgtcg
     1501 tcagtaatgc aggccgaccg tcatctccag gttttccccg ccatagggaa agctggcggc
     1561 cgcaaacgag ggagccccca gcagggcgct ggcgtcattt gaaaacccgt agccctccac
     1621 cgggatgccc agccagttca catccagttt gccgttcatg ttttcatcat gcacgacggc
     1681 cagggcataa ctgcccggcg cgatgtcttc aaagtcgcag cgtgcctgcc tgtcccggac
     1741 cttgatgacc atgatctggg tggcagcgtg caagtattcc gtcggaaacc ccaccgacga
     1801 ctcgaacagc gcgcaggcca cggcgcctgt gctgctccgg atgtccagaa tcttcacgtg
     1861 aatgccgggg caggcggtct gggccagggc ggcggcggga agattccccg ccagcagcat
     1921 gacgcacccc gcagcgcagc ggacggcctg cgtcctcgca ttctcgatgg tggccgtcag
     1981 cgcagacttt cgaacgtctg aatcaatcat gatcatcctc gcagttccgg accgcatgtc
     2041 tgggtcgatc gcagcaggac ccggggccgg ctgtccgggt ccgtcgccag gtgacggttc
     2101 agggttctgg cgttccggtg tcccggaagc cgcgcttcaa ctccgctctt gcggcgctgg
     2161 caagagcctg gtatttggtc cggaatgcat cgaggctccc ttcttcgagc tcttccagat
     2221 cgagcaaggc gttgtgtgcg ccctgtgttg cgcgaatcag ttcatcgagt ttcacctgga
     2281 tggcttcggt gtccctattc tgcgtgttct ggatcagaaa aaccatcagg aaggtcacga
     2341 tggtggtgcc agtgttgatg accagttgcc aggtgtcact gaagccgaac agggggccgg
     2401 tgacgatcca cgccgcgatc acacccaccg cgagcacgaa caccctgggg cggccgcaga
     2461 agtgcgccgc ctgtttcgcg aaccgggagt accagacggt gttattcatt taaaacctca
     2521 ttgtttgaaa ggagtcggac aacagctgca acgacgggcc ctccggtgcg tcgtccgcgc
     2581 caacgacccg ctccggcgat cactcatgca aacaccgcca cgcgcaaagg ctcgcgcttg
     2641 cctttggcca ggatgtcgcg ctgctgcatg ggcgcaaggc ctgcggcgtc gcaggcgacg
     2701 atgctcacca gcaattcgcc gggcgcggcg gcatcacaca ggcgttgcgc ggtattcacc
     2761 gtgtcgccta tgaaatcata ggccttggcg ccctcgccgc cgagcacccc ttcgacgacc
     2821 tggccacaat gcacgccggc accggccgcc agattcgaag ctgccagcag cttgcgcgtc
     2881 gaggccagca tgcgcgagcc cgcggtcacg gcctgggccg catccgcaaa cacggccatg
     2941 acttcatcgg cggtgtattt gagcttgatc ggagggtgct cgttcagcgc ctcttccgcc
     3001 gtccggtaat aggcattgag catgcggacc acggcctcgg gcgtctgcga ttcgctccag
     3061 gcggtaaagc cgcgaatgtc catgaacaag atggcgcgac ccacgcgctg cagcgacagg
     3121 ctgcgctcgt cagcaatcgc acggtcaaga atgccacgcc caagcgacca ctccgaatac
     3181 tggcgcaggc gcgccgtcag cgagcgcacc gtggccagtg agcggcgcag attgatgtcg
     3241 gcaaagccca gcagcacccc gagcaagagc agcaccacgg caagaaagtc atgcgcgcgg
     3301 tcggcaaaga agacgtcgaa cgacagcgct gtgttcttgc tgcgggcctc gaacagcgca
     3361 tacatcacca gcgggatcgc cgagcgcagt acgttctctg ccaacaccag aggggccgcg
     3421 ccccccgctt gcctggcctg cgccgattgc agcagggcga agccgagcgc aaaagcccag
     3481 taggcctggt gatgccactg ctggaagaaa gcggcgccct caaggcccgt ctcgaccagg
     3541 gaataaagca gcgggccggc cagattggcg agaaaaggcc gcggccggcc gcgcagggcc
     3601 aaccactcca tcagccaggc ctgcccgatg cccgaggcca gcaacagata ggggtcggcg
     3661 ccggcaaaat aaccggggcg cgccagcagc gcctcgagaa tcagcaatac cagtggcaca
     3721 tgaccactgt gggtgaagaa gttgcgcgca gcgcgtgcga aaagcgtttc agcagcgttc
     3781 attcggaaat acctgggctc ctgcctgcca tggtgtgtcc cgggtcagga gaggctggca
     3841 tcgggaagcc cgatgttgca ccacatgtgg atcttggaga cgtaggccgg acctgtggcg
     3901 acggcaaagg gctcgacacc ataggcaaca aagccgcagc tttcatacaa ggcccgggca
     3961 gcgtcgttcc cctgcgtcac cgtgagctgg accagccgga ccccgggacg ggcgcccgcc
     4021 tgaaccagca cggcctgcac cagactgcgc cccagcccct gctggcgaca ccgggcgggc
     4081 acatacatgc cgaagagggc ggccttgtgg cgcagcttct cgcgcgtctc gaacgacagg
     4141 ccggccacac cggcgagccg gtcgtcctga aagacaccga gcacccgctc tgcggccagc
     4201 ggcgcttcgc tgagccgcga ctgccaccac gacggcggca gggcggcgcg ctcggcgacg
     4261 ctggaagtga aagcgtcggg atgcagggca taggcctcga gcatgagcgc ccggtagtcg
     4321 ggcgcgtggg caggccggag ttgctcgatc agcatgaaga gacccctttt tcctcgccgg
     4381 cccgggggac cgcgtttgct attaattcaa tagctgcttg cacccgccca tcaagggctg
     4441 gtgtccaaaa acacataaaa acttgaaggc agctcacttc atgctgtccc gcagccgcgc
     4501 aaacaccttc cagaacgccg cctcctgcgt cgtcgcaaag ttgatgcgca tcagggtgct
     4561 gggtttgcgc accgcgtgaa acagcgcacc cggcgcgatc aggtaaccct cgtcgagcat
     4621 gcgctgcgcc agcgcgtcgg tgtccacgcc ggtctccacc cagccgaaca ggccggcggg
     4681 ctccgccgca aaactgcagc cgtgcgccag cgccagcttg acgctgcgcg cgcgcgccgc
     4741 gtccagccgt gtgcgaatgc gttcggcatg gcggcgcagc tggccctggt cgatgcacca
     4801 ggccagcgct ttttccagca gcgagggcgt ggtcagcgtg cccagcagct tggtgtcgag
     4861 caggcgctcc accaggtcgg gcggcgcggc catgaaaccg acgcgccagc cgggagcgag
     4921 gatcttggca aaaccgctga cataaatcgt gcgctgcagg ccgtcgagcg cacacagccg
     4981 cgtcgcatgt tcgggcgcca ggtggctgta ggtgtcgtcc tcgacgatgt ggaagttgtg
     5041 ctggttggcc agctgcagca cgcggtgcgc gctgcccggg gtcaggcaat aaccggtcgg
     5101 gttgtggaac acgctgacgc tgacaaacag cttcgggctg tgcagcgcgc agtactgcgc
     5161 catcacttcc aggtccggcc cgtcggcgcg ccgcggcacc ggcaggatgc gcatgcccag
     5221 cgcatcgagc cgggcaaact ccacggccca gccgggctcc tcgaccatca cgcagtcgcc
     5281 ggcgcgcagc agcgtgcggc tcacgatgtc cagggcatgg gtggcgccca cgctggtgat
     5341 gatctgttcg ggcagcgcat gcacattgag cgcgctgagt ttttgcgaga gcgcgcggcg
     5401 caggccgctg tcacccatgg gttcgccata actgagtgaa aactcgcgca gcgcgctgcc
     5461 gctggtgacc ctgcgcaccg ccgccggcat gaagctggtc tccagccact cggtcggaaa
     5521 cacacccatg cccggttgcg gcttgttgct gaccttgtgg aacatgccgc ggatcagcgc
     5581 ggtggcatcc accggcgcct gccggctcgc catccaggtc gcggtgctcc agctgctcag
     5641 cggcgccggg gtgtcgggcg cacttgcctc cagcgccccg gttgctccgt tttttgtagc
     5701 gacacgcgct gaatgggcgg gcgccaggac tgcattgaac gatgcatcgc ggacaaaaaa
     5761 cccgcggttc ctgcgcgcca ccaccaggcc ctgcgcctgc aactggtcat aagccgcgac
     5821 gacggtggac acgctgacgc cctgctgctc ggcacacagg cgcaccgagg gcaggcgcgc
     5881 gccgggcgcc agcaagcggc tgcgaatacg ctcggcaaac cgggcgctga gctgctcggt
     5941 cagggactgg gaggcggttt tcatcaacat ggcggactcc ggcggccccg gtgcaacgct
     6001 gcaccagcga cctgtgtgct gataatttga ccagcacagt ttgaaaattt gtcactacaa
     6061 ctgtattggt agtgtgctgg cttgaactat aaagtctgag tcatgaattg gcaagaattt
     6121 acagccctgc tggtgctggc gacggcgatg agcttttcgc ctggccccaa caccaccctg
     6181 tccaccgccc ttgcggccaa ccacggcctg cgccgcgcca tgccttttgt ctgcgcggtg
     6241 ccggtcggct ggggcgtgtt gctgagcctg tgcgcgctgg gtttgggcgc gctgctgctg
     6301 gcgattccgc tgctgggcct ggcgatcaaa ctcatcggcg tggtttacct gctctggctg
     6361 gcctcgaaaa tcgcgcgcac ccggcagttg ggccagctca gcgaagccga cgcggaacgc
     6421 atgaacgtcg gcttctggca gggcgcggca ctgcagttcg tcaacatcaa ggcctggatg
     6481 ctggcgctgg cgatcgtcag cggctggatc gccgggcgca gcgaccccgg cctgcgtttt
     6541 gccgtggtgt tgccggtgat gctggcctat gcctttgcca gcaacctgac ctacgcgctg
     6601 gtcggctcgc tgctgcgcga gtggctgtcc ggccccgcag gcagcggtcg gcgtttggtc
     6661 tggttcaacc gcgccatggc ggccgtgctg gtggccaccg cgatctggat gctcttttta
     6721 tgacgcaaca acagatcaac aagggcctgt ggctgggtct gctgggcatc gtgatttttg
     6781 cggtgaccct gccgatgacg cgactggccg tgggcacgcc cgaggcgccg cagatgtcgg
     6841 gcgtgttcat cgcgctgggc cgggccgtgg tcgcagccgc gttgtcggcc gtgtttttgc
     6901 tcgccacgcg cgcaccgctg ccgcagcggc aggactggct gccgctggcc atcaccgccg
     6961 gcggcgtggt gttcggcttc ccgctgttca cctcgatcgc catgcgttat gtcgaggcca
     7021 tgcatgccag cgtgatcgtc ggtgtgttgc cgctggccac tgccgccgtc ggcgccctgc
     7081 tgcaccgcca gcggccctcg gccggtttct ggctgtgcgc ggccctcggc agcgccctgg
     7141 tggtggtgtt tgccgtgctg cgctccggcg gcggcgccgg cctgtccatc catttcgcag
     7201 accttctgct gctggccggc atgttgtgcg cggccgtcgg ttacggctac ggcgcgcggc
     7261 tgtcgcagca catgcaggcc gaacatgtga tctgctgggc gctgctgatc tccctgcccc
     7321 tgacgctgcc tctggcgctg ctgagctggc cccaggcggc gatcaagacc agcgcctggg
     7381 ccggctttgc ctatctcggt gtgttttcga tgtggctggg cttttttgcc tggtaccgcg
     7441 gcctggcgct gggcggcacg gtacgtgtca gccaggtgca gctggtccag ccctttttgt
     7501 ccatgctgtt tgcggtgccg ctgctcggcg agcgcctgga cgcggtcacc gtgggttttg
     7561 ccctggccgt gatcgccacc gtgtttgccg gcaaaaaaat gcccgtccac accgtcacct
     7621 tgaaggccgc cacatgaaga tgagccacat tcctttcggc acgaccgact ggtccaccgt
     7681 ggcgcgtacc gagcattcgg cacaaagcgg ccaggccttc tggcgtacgc agcagtttgg
     7741 cgaggtgcgt gtgcgcatgg tcgagtacac gcccggttac gtgtccgacc actggtgcag
     7801 caagggccat gtcctgctgt gcctggcggg tgaactgcac accgaactga acaacggcag
     7861 tgtgcatgtg ctgctgccag gcatgagtta ccaggtggcc gatggggccg aggcgcaccg
     7921 gtccagcgcc cccaaaggtg cgacactgtt tatcgtggat tgacgacaga aaagagaaca
     7981 accatggaaa gaagcaatat gcactggacc ctggccgcac gcgccgaaaa aatgaacccc
     8041 tcggtgatcc gcgagatcct caaggtcacc gaaaagcccg gcatcatcag ttttgccggt
     8101 ggcctgcctt cgcccaagac ttttccggtc agcgcgtttg ccgaggcctg cgccaaggtg
     8161 ctgcgcgaag acgggccggc ggccctgcaa tacgccgcca gcgaaggtta cgccccgctg
     8221 cgcgagatgg tggcggccat gctgccgtgg aacgtggacc ccgcgcaggt gctgatcacc
     8281 accggctcgc agcagggcct ggatctgctg gccaagatcc tgatcgacaa ggacagcccc
     8341 atcctggtgg aaacgccgac ctacctcggc gcgctgcagg ccttcacgcc gatggagcca
     8401 caggtggtga gtgtggccag cgacgacgaa ggcattgatg tggacgacct cgcacgcaag
     8461 gccaaaggtg cgcgctttct ttatgtgttg cccaatttcc agaacccgac cggccgcacc
     8521 atgagcgaag cgcgccgcgc cgcgctgtcg gcccgcgccg ccgaactggg tttgccgatt
     8581 gtggaagaca acccctacgg cgacctgtgg ttcgacacgc cgccgccgct gccgctgagc
     8641 gcccgcaacc cggatggctg cctgtatctc ggttcgtttt ccaaggtgct ggcgcccggt
     8701 ctgcgtctgg gttttctggt cgcaccgaaa gcgctgtacc ccaagctgct gcaggccaag
     8761 caggcggccg acctgcacag ccccggcttc aaccagcgca tggtggccga ggtgatgaag
     8821 gacggcttcc tggaccgcca tgtgccgacc attcgcgcgc tgtacaagtc gcagcgtgat
     8881 gccatgctgg ccgcgctgca gcgcgagatg ccggctgacg tgagctggaa cacacccgac
     8941 ggcggcatgt tcctgtgggc gcggttgccg gccggcatga acgcggtggc gctgctgccc
     9001 aaagccgtcg acaaaaacgt ggcttttgtg ccgggcgccg cgttttacgc cgacaacgcc
     9061 gacgaacgca ccctgcgcct gtcctttgtc acggccagca tcgcgcagat caacacgggt
     9121 attgctgcgc tcgcggcagc gatccgggag agccgtcccg gcacggccat ccttcgacag
     9181 gctcaggacg aacggactgc gacgagcgtg ggggccaact agatgctgaa gatctggggg
     9241 cgcatgagct ccatcaacgt caagaaagtc gtctggaccg cgcaggagct tgcgctggat
     9301 atccagcgca cggaagccgg cggcctgttc ggcgtggtga agacccctga gtacatggcg
     9361 ctgaacccca actcgctggt gccggtgatc gaggacgggg actacgtgct gtgggagtcg
     9421 aatgtcatcg tgcgttacct cgccgccaaa cattcttccg gccagatgta tccgaccgac
     9481 ctgcgcgagc gttttgacgc cgagcgctgg atggactggc agcagaccac gctgaacccg
     9541 gccagccgcc ccggcttctg gcagctggtg cgcacgccgc ccgagcagcg cgacgccgcg
     9601 ctgcttgacg agtccaacgc cgcggtcgag gcgctgatgg cgatgctgga cgcccacctc
     9661 gcgcggcgca gcttcatggt cggcgagcgg ttcaccgtgg ccgacattcc cgtggcctgc
     9721 gaggtgcatc gctggttcgg cctgccgcaa ccgcgccaga gccggcccca catcgaacgc
     9781 tggtacgaca gcctttgcgc ccggcaggcc agcaagggcg tgctggatct ggcactgtcc
     9841 tgacaacttc cacctttcac cctttcacct tctgatccag caccatgaaa accaggccct
     9901 tcaaacaagt cgacgtcttc accgaccagc cctacttcgg caacccgctc gccgtggtgc
     9961 tggacggcag cggcctggac gacgaagcca tgcagcgttt tgcacgctgg accaacctct
    10021 cggaaaccac ctttgtgctg ccgcccgccg atcccgcggc cgactaccgc gtgcgtatct
    10081 tcacgccggg cggcgaattg ccttttgccg gccaccccac gctaggcagt tgccacgcct
    10141 ggctgcaggc cggcggcacg cccaagcgcc aggacttcat cgtgcaggag tgtcaggtcg
    10201 gcctggtgaa catccggcgc gaaggagccc ggctggcttt tgccgcgccg cccctgcagc
    10261 gctcggcgcc cagcccggcg gtgctggcac aggtggccgc cgccctcggc ctgaaagcgc
    10321 agcacatcct ggccgcgcaa ctgctcgaca atggaccagt ctggctgggc ctgctgctgg
    10381 acagcgccga caccgtgctg cagttgcaac cggatcacgc cgtactcaag aaacatggcc
    10441 tgaaagtagg tatagccggc gcccatcaag cgcagacagc tcctgttttg atagcacgcg
    10501 ccaaccgcga ggcgcgcgct ttttccggca gcacgcccac cggccccgca gaaacagcca
    10561 cggcagactt tgaggtgcgg gcttttgcag cgcccatcgg catcgaggaa gacccggtca
    10621 ccggcagcct gaacgccagc ctggcgcaat ggctgatcgc cgaaggccac gcgcccgagc
    10681 gttatgtggc cgcgcaaggc acttgcctgg gccgcgccgg ccgcgtctac ctgacgcgcg
    10741 agacggatac ccgggggtcc gcccaggtct gggtcggtgg tccatcggtc acctgtatca
    10801 acggcacggt gacgctgtga gccgcgccca ggtcgatcac ctggtcgtcg tggccgacag
    10861 cctggcgcag ggcgtggtct ggtgcgaaga gactctgggc atcacgcccg gccccggcgg
    10921 cgaacacccg ctgatgggca cacacaaccg cctgttcagc gtggccggcc ccgccttccc
    10981 gcgcgcctac ttcgaaatca ttgcgatcca gcccggcgtc acacccctca gggcggccgg
    11041 cacgcagcgc tggttcgaca tggacgaccg cgaactgcag gcgcgtgtgg ccagtgaagg
    11101 cccgcagctg gtgcattttg tcgccagcgt gcccggcgtg gtctcggccg tcaaggcact
    11161 ggccgccctc gggctggacc gcggcagcgt cctggaagtc gcgcgccaga ccgcgacggc
    11221 cctgctccag tggcgcatca ccgtgcgcga cgatggccag cgactgctct acggcggcct
    11281 gccaaccctg atccagtggg gtgaagccgg cgaggacccg gcccgcgccg cccacccact
    11341 ggacgcgatg ccggcgtctg gcgtcacgct gcaggcgctg caggtcagcc acccgcagcc
    11401 cgacaagttg caggccgccc atgccgccat cgggctggcc ggtgtgacgg tgacccaggg
    11461 tgcgcccaat cttctggcca cgctgcagac gccgcgcggc ctcgtcacgc tcgaatcgaa
    11521 aggtgtctag atgttgacaa cacaggaact cgcctggttc gccctcgtcg cgctggcgct
    11581 ggtgctcaca cccgggccca acatgattta ctgcatctcg cgcaccttgt gccagggacg
    11641 cgctgccggc atggtctcgc tgggcggtgt ggccctcggt tttgtggtgc atctgctggg
    11701 cgcgtccttc ggcctgagcg ccttgcttct ggcggcgccg ctggcgttca ccgcgatcaa
    11761 ggtcgccggc gcgctctacc tgctgtggct ggcctggcag gccgtcaaac ccggcgggct
    11821 gtcgccgttt gaaacccgta gcctgccgca cgacccgccg cgcaagttgt tcctgatggg
    11881 cttcatgacc aatttgctga accccaaggt cgcgatgttt tacctgtcct tcttcccgca
    11941 gtttttgcac cccgagcgcg gtcaggtcct gctgcaaagc ctgagcctgg gcgccgtgca
    12001 gattgccgtg agcctggcca tcaacaccgt gatcatcttt ttcgccgccg gcctggcggt
    12061 ttttctcaac cgcaacatcg cctggatgcg cgtgcagcgt tatgtcatgg gcagcgtgct
    12121 gggcctgctg gcgctgcgcc tgctgagcga aaaacggaca agcacatgaa cgccattctg
    12181 aaagacctgc gcagcagccc gctgccttca ctcgcagaca tcgaagccgc ggcccgcgtg
    12241 gtttaccgcg agttccaggc cacgccgcaa taccgctggg gcctgtcgag ccagcggctg
    12301 ggcaccgact gctggatcaa acacgagaac cacacgccgg tcggcgcttt caagatacgc
    12361 ggcgggctga cttatttcga tgccttgagc caggccggca cgctaccgaa ggaagtcatc
    12421 agcgccaccc ggggcaacca cggccagagc ataggctggg cggcacgcgc gcatggcgtg
    12481 gcctgcacca tcgtggttcc gcacggcaac tcgctggaga aaaacgccgc catgcgcgcg
    12541 cttggcgtca cgctgatcga acacggcgaa gacttccagg aaagccgcga gtacgcgatg
    12601 cgcctggcga ccgaacgcgg cgcacacatg gtgccgagtt ttcatgccga cctgctcagc
    12661 ggcgtgagca cctactggtg ggagttcatg ctggcggtgc cgcaactcga tgtggtctat
    12721 gtgccgatcg gccagggctc gggcgcatgt tcggccattg ccgccaagct ggcactgggc
    12781 cgcaagctgc gcatcgtcgg tgtggtcagc gcccatgcca ccacctatgc cgactcactg
    12841 gccgcgggcc gcgtcgtcga agcgccggtc agcacccagc tggccgacgg catggcctgc
    12901 cgcatcgcgg acccggccgc actcgacatc ctgctgccgc acctggacca catcgtgaag
    12961 gtcaccgatg ccgaggtcgc gcaggccatg cgcgacctgt atgccgacac ccacaacatt
    13021 gccgagggtg cgggcgccgc gagttttgca gccgccatgc aggaacgcgc gcaactcaag
    13081 ggccaggtgg tgggcaccac gctgtgcggc ggcaacgtgg acagcgccac tgtcgccaag
    13141 atattgagcc cccacgcttc tcgcttcgcg tagtgcgctg ccccccaggg gggcgctgcg
    13201 cctgcggccc ggcgaagccg gttccgcggc cccggctgga ctgactactt ttaacggtgg
    13261 tcacacgcgt gactgggtgt ggcttcggtg ggcgccacaa tttgcccatg ggaacccttt
    13321 acctcgtgcg ccacggccag gcctcgtttg gcgctgaaaa ctacgatgtg ctgagcagca
    13381 tggggcatcg tcaaagcgtg cggctggggg agtacttcaa atccaagggc ctgaccttcg
    13441 atgcggcgct gaccggcacg ctgaaccgcc agatccagac ctttgccggc atctgcgaag
    13501 gcatgggcac ggcgcccgat gccttgaggc aaaccgggct caatgaatac gatgcggaag
    13561 ccgtgatcgc agccatccat ccggccaaac tcgaaaaacc gacctcgccc gaaatggtcc
    13621 gcagccactt ccgcctgctc aaggacggcc tggcgcagtg gatggccggc gtggtcagcc
    13681 cgcgcggcat gcccagttac accgattttg tggcgggcat caccggggtg ctcgaccacg
    13741 tcaagaccag ctacaccggc aacgtgctgc tggtctccag cggcggcccg attgccacgg
    13801 ccgtgggcca tgtgctgggc accagccccg aggccaccat cgacctgaac ctgcgcatcc
    13861 gcaactgctc gctgaccgag tttgccttca cccccaaacg ccacatgctg gtgacctaca
    13921 acacgctgcc acacctggac gcaccggaac acgcggactg gatcacctac tcctgaaccg
    13981 gggcaggcgg caggggcgcc gcccgcgtgc cttcgcgcag ccaggccaga tacccgaccg
    14041 acccggccgc aaccggcaac tgcagcagct cgggaatttc atacgggtga tgggcgcgga
    14101 tgaagtcctc cagcgcaggc acccgggccg tgcgcgtctt ggccagcagc aggtattccg
    14161 cctcgcggcg gctgtcaccc ttccagacat aaaagctggt cgtcgggacc acctgaacac
    14221 aggcggccag cccggcctcg accatgccgc cggccagcgc ctgcgcggcg gcctcgctgt
    14281 ccacactggt catcagcagg caaaaatcgt tcgatgtttc catggcccgt ccctccgtga
    14341 aaagaatcag ccgaaaagcg gcatcaggtg gatgatgccc atgcctgcca ggccctgcat
    14401 cgtcacgtaa tgccagagca gagcggtgtt gtccagcgag gcgcgggcgt cgctgtgcag
    14461 cttggcactc caggcgcgtg cacagacata caagcccatg atgagcagca ccgccacatg
    14521 aaagccctgc caggccagca tggccgcgac cgtcgcgctc caggcatcgg ccgtcggact
    14581 cagaacggcg agctgctggc cccggccgtc caggccgaat gccgccagca cacacagcac
    14641 ggccagcagc accagcgccc agaccggcgc gcggcggcca cgcgccagca gtacctgcgc
    14701 gcccagcatc gcagccgaac cagccagcag caaggcggcg gatgccagcg gccagaaggc
    14761 gcccggcagg ctggcgccgg gcggcgggca gacgtcggcg gccatggcca catgcacatg
    14821 cgcaaacacc agcgaggcaa acacggtggc atcgacacac agcaggatga tcatggccca
    14881 ccaggaatgc gaggccatgc cgcgcgcgcc caccggcagg aagatgccgt cggccacctc
    14941 ggccgtcgcc gcatgcggcg gctggtcggt ttgccacagc caggccacga tggcgacgat
    15001 ggcggtgatg ccacaggcaa aggccaacac cacctgcgcc accgtcagca gcaaaaagaa
    15061 accggccgtg cccgccgccg cgatgaacgg ccaccagctg tcgccgggca gcaccagcaa
    15121 atgttgcggc acggcgtcgc gccagctggt caccagggtc tccctcccgc ccgaacgggt
    15181 gccgggcagc cagtgccggc cggccaccac ctcggcggcc agggtgggcc ggtcccacag
    15241 cggctcgcgc gaagcgatct gcggaatgct gcgcatgccg tagtcctcct gcggcagcca
    15301 ttccagcgtg ggcgcgttcc acgggttgtc gtggtcgatt tttttcctga acaacacgcg
    15361 cgccgcatcg atgaaaaaca gcagcacgcc cagcgccagg atgaaggagc ccaggctcga
    15421 cagcaggttc agcaagttcc agccgaggtc gcccgcatag gtatagacgc gccgcggcat
    15481 gcccagcagg ccgctgatgt gcatggggaa aaacgccagg ttgaagccgc cgaacatcaa
    15541 gccgaagacc cagcgcgcgc agcgttcgga caggcggtgc ccgttgaaca ccggcgccca
    15601 gtagtaaaga cccgcaaaca ccgggaacac catgccgcct atcagcacgt aatgcaggtg
    15661 cgcgacgatg aagtaggtgt cgtgcacctg ccaatcgaaa ggcagcaccg ccaccatgat
    15721 gccggtcagc ccgcccagca cgaaaatgaa atgaaagccc agcaaaaaca gcgtgggcgc
    15781 cgtcatctgc acgcggcctt tccagaaagt cgcgatccag gagaacacct gcaccgcgct
    15841 cgggatcgcc accgcaaaac tcgcggccga gacaaacagc atggcatgcc ggctgaggcc
    15901 cgcggtgaac atgtggtggg cccacagcgc aaaactgatg aagccgaccg ccaccagcgc
    15961 gaccacgatg gcgcggtgac cgaccagcgg cgtgcgcgcc atcgccggca ccatcatgga
    16021 gaccatgccg gcggccggca ggaagatgat gtagacctcg gggtggccga agaaccagaa
    16081 caggtgctgc cagagcaccg ggtcgccgcc gcgcgcggcg atgaagaaag gccagtcgaa
    16141 ggcgcgctcc agttccagca gcagggtgcc ggcaataatg gccggaaagg cg
//
LOCUS       DAIDFG010000034        16066 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_34_length_16066_cov_17.116420, whole genome shotgun sequence.
ACCESSION   DAIDFG010000034 DAIDFG010000000
VERSION     DAIDFG010000034.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 16066)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16066)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16066
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34_length_16066_cov_17.116420"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         3195 a         5130 c         4944 g         2797 t
ORIGIN      
        1 taccctcagc tcaaggtggt caagctcgag cagaactacc gctccaccag cgccatcctg
       61 cgcgcggcca acaacgtgat tgcgcccaac cccaagctgt ttccgaagac gctgttcagc
      121 gaactgggtg aaggtgaacc ggtgcgcgtg gtcgattgcg acagcgagga gcacgaagcc
      181 gaacgtgtgg tggcacgcat ccagagcctg cgcgccgaag gcacgctgca ggccgtaggc
      241 ggccagcaca aggagtgggg cgacttctgc gtgctgtacc gcgccaacca catggccaag
      301 ccgtttgaaa aggcctttcg caaggccaac attccctaca aggtctcggg cggccaaagc
      361 tttttcgacc gcgccgagat caaggacctg tgcagctgga tggtgctgat ggtcaacaac
      421 gacaacaacg cggccttcat ccgcgccatc aagacgccca agcgcggcat tggccacacc
      481 acgctgggtg cgctgagcac ctttgccgag cagtacaagc tgagcctgtt cgagtcgctg
      541 ttcagcagct ccctgggggc ggtgctgccg gccaaggcgg tcggctcgct gcatgaattc
      601 ggccgctacc tcaacgacct ggaataccgc gccagacgca cggtgggcgc cgaggatgca
      661 cgcgctttcc tcaccacctg gctcaaggaa atcgactacg aaaaacacat ctacgacagc
      721 gaggacagcg agaaagtcgc cgccgcgcgc tggaccaacg tgatggattt ctgcgactgg
      781 atggcgcagc gttgcggcgg gcagatcgac gacagctccg gcgcggtggt ggctgccgaa
      841 accaagacca tgctggaggt cgtgcagacg attgccctgc tgtcgaccat cagcgaacgc
      901 gaaggcgacc agaacgtggt gacactgtcc acgctgcacg cggccaaggg gctggagtgg
      961 ccgcatgtgg tgctggccgg cgtgaacgag ggcctgctgc ctttcaagct cgatgacgac
     1021 gacggcacag atgcggccag gaccgagaac atcgcgcagc gcctcgagga agagcgccgc
     1081 ctgatgtacg tgggcatcac ccgggcccag tacacgctgg cggtcaactg gctgcgccgg
     1141 cgcaaaaaag gacgcgacac cgtcgccggc atccccagcc gttttattgc cgagatgggt
     1201 ctggacaagg ccacggtccg ggaagacccg cgtgaaaaaa tcagggccct gcgcgccgaa
     1261 ttcgccaaac gcgccgccga cagcgccgaa gcgaaccagc tcgccgaggc ggcccgggac
     1321 gctggttaag cccttctatt gcacacccga tgaaaaaatc accttcgctc acatttgctc
     1381 tgttttttat agctgtcagc gccctgtcca tgagggccta tggccaaaat gatacgcgac
     1441 aacagtctgg accggccagt ccggcggttg cggcctcctg cccacccgtc caacaattca
     1501 agcccgcgca aatccatggg acatggcggg cccggttcac gcctgcaccg gcgggtctgc
     1561 ccgctgacgc caccctgcaa ctccagcggc acgccgaatt cagcgagagc gtggccggct
     1621 tcgtcaaccg cgagctggcc cccacagccg gcaaacccgc ccagggcggc cacgccgcca
     1681 gggcgcagct ggccggcgat ctggaagacg ggtacctgac cctcgacgag tcctccaacg
     1741 gcatcagcat caccggagcc tggaacggca aaatggtgga aggcacatgc ggaagaaaaa
     1801 tcacgggtac ctggaaagac atgagcagca gcgcgccgcc cgacgcgccc gatgtgccgt
     1861 tcacactgga aaggttgtcc ggatggtagt catcatcaca cccgaacgca ggcgccttgc
     1921 cgcaccgtgg ctgtatgccg cgctgctggt cgcgggcgcg gcgcaggccc agaccggcag
     1981 caccggcccc accgtgcagg agcgcaacaa ccgccagggc tggcaggcct gccacgccat
     2041 gagcggtgat gccgctgcgc aactggcctg tttcaagaac tgggcagtct cgcaattgcc
     2101 ggcggacacg ccgccgccca gcctgaccac cgcaccggcc aatgccgaca ccgggcaaaa
     2161 gtctgccgag gtcctgctgc tgccggccat gaacaccgaa gccccggatg gcaagaaaat
     2221 cggctgccgc aacaccaagt actccgagtt gtcccgcttc tgggagctgc agcgcggcag
     2281 cgactgcgac accttcggcc tgcgcggcta ccgccccatc agcgtgatgt ggtcgggatc
     2341 gaacagcatc aacaaccagc cgagctcgga ggcaccgggc tactcggcga gcacgccctc
     2401 caactacaga cgcaacgaga cccggcttca gctgtcagtc cgcacaaaac tcgccaaggg
     2461 catgctggcc tcgggcggcg acgatgaatc cggcatcgat tcgctgtggt tcggctatac
     2521 gcagcaaagc tactggcagc tgttcaacag cgcggcttcg cggccgttcc ggaccaccga
     2581 ccacgagccc gagctggtgt acatctatcc acaccagatc gatctggccg gcggctggaa
     2641 gtaccggctc agcggcctcg gcctgaacca ccagtccaac ggccagggtc tgccgcagtc
     2701 acgcagctgg aaccgggtct acctgatggg cgcagccgaa aaggaactgg gcggcgacag
     2761 ccggctggac ctgcaggcac gcgtctggca acgcgtgcgc gaacgctcgg ggcaggacga
     2821 aaaccccggc atctccgact acatcggccg ggccgagctg gccgggcact ggcaaatcaa
     2881 ccgcacgcac accctgggcc tgacgctgcg ccattcgctc aaatccgaag cgcgcggatc
     2941 ggcccggctg gaatggatga aggcgtccac ctcgattgcc gacagcgccg ccctgcgtta
     3001 ccacgtgcaa ctgttcagcg gctacggcga cagcctggtc gactacaacc gccgccgcac
     3061 ggtgctcagc gtcgggctga gcctggtgga ctggtagcgg gctgacaggc gcgcggggtg
     3121 tcgggcaagc gcaagctgtt tgaacctgcc gacctgccgg cctgagtcgg cactgcgtcg
     3181 caccggcacg ccgtatccgc tacagttttg atagcctcct gcgcccgcca acaaagggcc
     3241 agtggcctgt tttatatata aaaaccgggt cccaccggaa gcagatcccg ggacgcgttg
     3301 acatcactcg atacgcggtg ggagcgctgc cctgctcgct gccgaaaatt ccggcagagc
     3361 cacacccatc ctctgcgccg cagcgagagc cgccggcttc acaggacggg gtggcaaggg
     3421 cggggcagcc cgcaccggca gcggcgcagc aggacggggc ggcaggggcg gcggcaccac
     3481 acgcccgccc gagcgcagca aagtgggcgg cggcgggttg tcactgccaa aacccacaat
     3541 gcgcgagcgg tccacctgcg gcagcggcgt cttcgaggcg tccgtggtga ccatgtcgat
     3601 catgccgtcc gtcacatgct gcttgaggtt gctggctttt tcgctggcgc tcattttttc
     3661 ccaggcagcc agcttgatgc ccttgtggtt ttgcatgcct ggaatttcaa accgggagcc
     3721 ccggacaaac tggccggcct tggcgatcac gggtttgaca gcggcgacca ggcggtctgc
     3781 atcaaccacg aagttgggcg tgccgaagtc ggcgacagcc atgttgatga gtgtgctgtg
     3841 atcctgcacg gccacgtaat agcagcccag cagcgggcag ctgcggaaca ggctcaggtc
     3901 cagcgtgccc tgggccagca gccggttggc cctctggacc tcttcacaat cctgcacaaa
     3961 ctcgacgatg tgctggatcg ccttggcgat ggtctcggcg agtccaatcg ccgggccgct
     4021 ggccgcgccc aggtccagcg cgctgcacag ggtcttggcg gtgaaggccg cgcctgcaat
     4081 cgcggtcgtt gccatggtgg agttgatttc ccgatcgatc agcacctcga gcgcctgaaa
     4141 agcagccagc gggtcgcccg ccgcaatcgc gtaggtgccg acttcatgca tttgcgcggc
     4201 ctgccacttg ccgtacacga tcttgcccac gcccaccgcc gtgtcgctcg ccgaggcaat
     4261 gatgcccagc gcaggggcca tgctcttggc aaagtcggcg acgcttccca gacccagttc
     4321 gcgcatgatc atcgcggggt ccagggcgcc gcaaaccgcc ttgaccatct cgtcgaattt
     4381 ttgtgtgatg gccgttccgg ccacgacgtt gaccccctgc gccgtccggg tgccggaccg
     4441 ggtcacattg atgtcgcgga tattggcagc cgcgccctgg gttttggaga gcagggtttt
     4501 gatttcgctc gcgtgttttt tcttgttgaa acttttcagg ctcagctctt tgccgacgaa
     4561 catctccttc agggcgagct gctgcagcgt caccatttcg cgcatcgcca gcaggtcggt
     4621 ctcggtgagc cgtcgctggt ccacgttgtt gactgcccgg tgcaggttgg tgagcgcgcc
     4681 tttcttgttg cgcacgcttt gggtccagtc cttgccgcgg cggctgtggt cgaaccggta
     4741 gtcgtccagt gcctgcttga tcctctgccg cgactgctcc gacttgtcgc tgtcgtagac
     4801 gaatatcgcg aggtcgaccg ccttgagttt gtcgctgcgc gcgtcggacc acgagtaggt
     4861 gtcccgcatc catgtttcat gccaaggaat tgttgccata tgaccgggtt ccccttctgg
     4921 ccgccccagt tcaaaccatc ccgccacggg atgattcacc gaacgactga aatttatcca
     4981 ggcagagcgc gacgtggccc gaccgaggac gttttataaa aaatcgagct caacttgccg
     5041 gcgcagacgg gctgcgcccg actcacctta ttcgcgcgat tccttgatgc cgagcagcga
     5101 aaaataccgt gcaatatcgc cctcttcgcg caccacttcc tgcatcaggt cggccagcga
     5161 catgcgcccc caagagacgg cacgcttgac caggtacggg gtcggactgt gcggctcggt
     5221 tttttccaga aaagccgcga ccgcctccag catgcggtaa gcatcctcgc ggctcgaaat
     5281 ggaaccggaa cgcagcagcg cctcgtgtgc agcgggaact gcgtcggtgt tcggagttgc
     5341 agcagccatg atgtcgtcct ggttgatggg gggaggcatt gtttcgtcct gcgcgggcga
     5401 tgcaaccggt tgcggccgcg ggtcgcgccc gtcgatcagg ctgtcgacgg cccgtccaag
     5461 gcgccgcaac atgtcgccga cacgcgccac actcgggggg ttggcgctca ttctttcgtc
     5521 gagcattccg gtcagggcat cccattttcc ggtcgctgcc tgaatctggt ccctgatgac
     5581 tgccagggcc agcaggttgt tcccgctggc ggcggcgttc atctcctcgc gcgtcaaggc
     5641 accggcacct gacgggcctg ccggtttttt tgcaacgccc ttttggttat ccgcaatcag
     5701 ggcctggtcc caatctgcca ggctgaccgc tgagggcatg cggtcaggca gctggagcag
     5761 ggcgatatgc agcttcagga ccagcggcat gttttcattg agccagacaa agggtgccgc
     5821 gcgggcatcg tcgtcaccgt cctcgatcag gggatgggcc gtgtcccaaa accgttcgac
     5881 caaccccgtc agcaggtcca ccgaagcgtt gaaaccctcg agctggtgct ggcgtgtcca
     5941 ggactcgcac agccaggccg ccacctggaa atccttggag cggctgccca gcagctcggt
     6001 gcacctggcc gagacggcct tccagtcggc ctttttgaga ggccgctccc actcgcccat
     6061 gggcaggctg gcgtcgtctt cctcgcgcgc ctggcgaatg ccgaggtaca gcgggtcata
     6121 ccgcatggac ggcccggccg ggtctgcccc gggaacaggt gccagcaggg cttcgatgtc
     6181 aggctgaaag cctgcagatg aatggttggt catgcgtgtc tcactgcccg ccaaattcgg
     6241 gtgcccgggt cgggaacacg ccgggccaga acaccggggt gcgcttgccg accggcgaca
     6301 gggccagccg caggtagacc cgggcgcgaa cctcctgcgg ggccaccctc gggttgtccg
     6361 gcgtgaagct gagcgggaac tccatacgca gcagttgcgc gcggccgtcg gcccgacccg
     6421 ccgcatcggg ttcgcgctgg cgctggatca tgcgcaccag ggaccaggtg tccgtgaacc
     6481 ggtaagaaac cgttttgcca tcggcagaca tatggggctg ctggggatca ttgacggcgt
     6541 tcaccgggga gtctttggca agccgcaagg tcagggtgat cggcgtgccc ggctcccagc
     6601 gcagcggacg ccgtgcatca cgcagcttga gagtttgcga gcccacctcc agggtccagt
     6661 cgatcacctt gttgccttca atttcggccg actggttggt cctgaactcc accgccatgt
     6721 cataacccgc aaccgctcct tcttccaccg gatacagcgg ggccatgaag gccctgacac
     6781 ggtcgaactg atcggcaaag cgccgggcag gcagcccgac caaggcaccg cggcttgcgg
     6841 cggatgtttc cttcagggtg cgggacatcc gctcggagtt tttgaggatc tggccaacgt
     6901 catcaaagtc cgcatccggc gcgtccttgg cgccaggcac cgcaaagggc tggcgacccg
     6961 ccagcaggcg gttgaaattg cccgagaaag ccgcccactg ctcctgctgc tcgccgacac
     7021 gcaattcgtt gcagcgtttg agcagcgcgc tgtagacctg caggtgtttt tcggcaaaat
     7081 agtcggccgg tcgcccgccc ggtgccttgc ccgccagttt ctccgagcag ttcgccctgt
     7141 cgaggtcggc tccggtggtg acaaggaact gctcgtaaac gaccaggctg ctgttcgggt
     7201 ttttcaggcg gtaacgttcc agttcacggt tgatggcctg ccagcgccgc gccagctgcg
     7261 attcggccgc agcactgtcg agcgaggcaa gatagatttc tgattgccgg cccagtgttt
     7321 ccgcgcggct gaactgcagc gccagggtct gcagcaggct ggggccgtct ggcacaccga
     7381 acgcctgcag cagcggaccg cgatcaccca gccaataact gaagtcccgc ccacggatgg
     7441 catacaggtc ggactgggtc aagctgtcgt cgacctgccg caggcgcgtc acggcatcct
     7501 gcgaaatcag gccgcgcaag ttgtccgcct tgcctttggc gccaagttcg gtgagcagtc
     7561 cctggacctt gaccaggcgg cttcgcgatg cgtcaaacgc cacggcatcg gccaccgcac
     7621 cgggatcggt cctgctgctg atcgacaagg cttcaaccgc gtggtcacca accaggcgtg
     7681 caaactggaa gttgacgaag gccgcaacgc tgggccgggt cgcaggcggg aacttgacca
     7741 ggccttcacc catgaagcgc ttgtgcgctt cactgagcgc caaggcctgg tccagcctgg
     7801 tgctgtccca taccagaatg ctctgcgcca cgatctcggg aatctcgcgg tcgctgggcg
     7861 gcgtcatgaa cggctgtgcc agcagccctg taagcgcatc gcgcagggca agcctgtcgg
     7921 cggacagcgc aaaacgcgcg tctttttcct gccagaccac acccgcttgt ccttcggccc
     7981 cgaggagtgc ttcaaactcg gtcctgaatt tctggaattc ggtgaccgca tgctggcgca
     8041 cctgatccga agcttccggc ccgagcagac gcctggactg ggtgatacgc atcagggcgg
     8101 cgtcataggc cgaacccagg ttcaggtcgg gcagtctcat ccagccgcct ttgccggtgg
     8161 ccagcaaacc ttcctgctcc ttgatcgccc tcaggatttc cttgtagccg ccgacggtct
     8221 gggtgtagcc gccaatacgg gcatcggtgg caaacagccg cgcaccctgc gccacaagct
     8281 gccgttcgga gaccagcaag tcgttgttca cgaacaggcg tctgtccagg gcattcattc
     8341 ccctgtccag cgaacagcgc accgcctgct ggatgtgcat cacgctgatg gctgccggcg
     8401 agaggctccc gggaatatcg ctgcccctga agaagcggat gctctgcgac atgttgcccg
     8461 gcaattcggc gcccagggtg tacttgacca gcaaccggag gtcgttggct tcgttctgat
     8521 tgggcttttg caggcgaacc atggccacaa tcgcctggtc cagccgctcg acggctgtca
     8581 ggtattgcag catggccgcg aactccggca gatcttccac ggccaggctg aacctgtgtg
     8641 gcgtacccga gatcgccgca aacccggcag gcgcagcgca ctcgccgcca atgatgagtt
     8701 ccgaggtgct ggcatcctgg gatacgccgg tcatgtcagc agcccgcgca tacagttcgc
     8761 gccgcagggt gttgatggca atctcgccga actcgcgctc tatgcgcgcg gccacacgat
     8821 catcgaggtt gtcgaaaacc tcccacgagc cgggcatgaa aaacgtccag gccttgtcgg
     8881 ttcccagctt ctcgatggca gccagcaggg aaagggtttt cgcccggtac cagtcggagg
     8941 cgataggttc accgcgctcg ctggcccggg cccggtagtt ggcatcggct tcgatttgcg
     9001 acagggccct ggccagttcg gggttgcgtt tgctcagttg caccgtggcc accagcaggc
     9061 ccacactcca gccgcccaga accaccagtg cagcccagcg cgccgcgcgg ctcagcaccg
     9121 gccgcgccag ctgttggcgg gacggcctgg ccacgccgta ctccatgaaa attttcttct
     9181 cgaaaagatc ccgcaggaag gccggttcgc gcatcagctg cgacaccagg tcggcctgct
     9241 cttccttggc ttcaccattc agcgccgccg cgacatcaag ggcgccggcc ttttgcgccg
     9301 attcgctgct gtcaccggtc aggtaaatgc cgcgaaagaa aaacggctca tggtaggcac
     9361 tgggccgcat gagttcgtcc acgtaaacct gcagttgcgc ccgaaccgca tcgatccggg
     9421 agggaagcag aaaaaatttc cctgtttctg cactgaccga atcgagcgca aagagttcgg
     9481 ccgaggtgtc cgagaccgtt ttcacgacgc tgtcgagcgc ctcgccgacc cagccgtcct
     9541 ggtaggtggt ggacagatcg taaggcgatg accaaccgag aatgccggcg cgcatgggct
     9601 cgggcagcgc cctggcgaag ggcgaaaacc cttcgatctg gtcgcacccg gaaaccacga
     9661 cgtagacggc aaaccgcatg gcaaagcggt tttgcgcgag ccagagccgg cggtgcgcga
     9721 ggcgggccag cttgccgagt tccagcaggg catccgggct gttgtccagc agcaaggcgg
     9781 ccggcacagt gatgacgacg gagtcaaagg gtctctgggg ccggtattta cggcacaggc
     9841 cgaggaactc gtcccacggt ttttccgttg catcttcgtc gtcgggcgag cccaggtagg
     9901 cgccctgcat gtcaatgaca atgcccttgt caaagaagtg ccaggcgatg ccctgggttg
     9961 cagcggcgga agcggcctcg gagctgagcg cactggccac gcccgactgt tcaatgggca
    10021 gttgccggtt gtccatgcct tcattgagta ccagcaccca gggaatgctg tagcgctcgc
    10081 tccgggaagc aatattgccc tcgatcagtt caaccgcctg cctgaaggag ttgcgcagcg
    10141 agtcaaaccg gagcttgacg atcttgggat cgctgccggg cttggcagcc gatcggcgca
    10201 cggcgaaata cagtaccagg cccagaacaa ccagcaccag gacaatcaga acgcccagag
    10261 acgccagaaa caggttgtct ttcagcatcg tcggcccccc ggttgcagtg gagaaggcgc
    10321 tttcattggc tgccccccgt tccggtcgtg ctgccggtct gcaggacctg acgcaccggc
    10381 caggactgcc acagccagag catttcagag atggccagca atcccacaac accgagaagg
    10441 aagaccaccg tccaacggct caatgtcggc atctttttcg gcgcaatatg cgacagcgtg
    10501 ctggcataag cgcgttcgga caggacacgg tcacggccgg agagatcggg ccgccgctgg
    10561 tagacaaact ggaacagctc ctcccggtag cctgcaaaac gatccgtgtc ggctgctccg
    10621 cggtatttgc cctggaaacc cagcgccagc gccgagaggt aaaggctggc gatcggccgc
    10681 aaggacggct cgcggctgga cagcagttct tctatgcgtt cgaaaatccg ctcgccggca
    10741 atgctggttc tgaagaggga ggcttccagc aaatgggtgg tccagcaatc gcgacctatc
    10801 caggccgtgt tgagcaggat ttcatcggcc agcgcggcct tgaggtaacg cgcgtcggca
    10861 atgctctcaa gttcaaaccg gttgccctcc cgtttggact ccagcgtctg cagctcgata
    10921 aggttttcca gatgtttgct gacatgctgg gcagccaggt ccgggtcggt ttcgcgcatc
    10981 tcgatcgcgc gggtctgggc cttcgaaact tcgtcgaaga aagagcggaa ctgcgtgatc
    11041 aggtggttgt cgtcctgttt gtctcgggtt tggcgtgcca ttgttcgctg ctttctgttg
    11101 gagcgtcagc ccttgacgaa gagaaccatt tcctgcgggc gttgcgcaga cgcactttcc
    11161 gtggagttgc tgacaatcag gggctcattg gccagggtca gggcggcgct ggcctgaatg
    11221 gaaaacaggg tgtatcccga gctggcccgc acgccgagct cctgggcaac ttcaatgtgc
    11281 gagcgcatgg caccgagtac gcgtctttcc ctgagcgacg cataagccga ctgggacccg
    11341 acaatggcgc cgttcatcca ggcgaccagg tctttttcgg gttgcccgcg caagcccacc
    11401 accagctgct tgtcgaccca ttccggcagc aggttgattt caaacgcacc atggcggtac
    11461 tcgaacttgt gttcccggta ttcctggctg atttccatca gggagtcctg caaagcctcg
    11521 agcaccgggt tgagcaccgc cagcggatcg gcatgatcgt actcgggcgg taccggcggc
    11581 agggccccgg gcttgagcat gctcagaggc ccgagcaggg cgcatagcgc cagatacagg
    11641 ggataagggt gcagactggg tgtgcgcaag acggcttcga cttgcggcag gccagtcatc
    11701 aggttgcgaa gccgctccct ttgctccagg taggccagcc tgtcctcggt tttggaggac
    11761 ggcacagccg tctgcttcgc cacaaatgca gccttgccgc gcaattggcc gacgaaggca
    11821 gacacccgct cccacaagac gccgtctttc ggcatctcga tcagcgcggg cagggtctcg
    11881 cccatcttga tgagttcgtt gtccttgaac accgtgccca ggcgcagcca ggtataaatt
    11941 cccgagggca catcacctgc aaccagcgag aggttgggca gcaaccgcgg aaggtcggca
    12001 ggcagtgcct ccgacacctc gtcttccacc tggcccatgg caaccgaacg gaaccgcgag
    12061 ggcgagcctt tgtcgcgcat gcttttggtc agcggcagga ccaggtagaa atccagaggg
    12121 gcgttgccca gcgcttcagc aaacggggcc agtttgagct cgagcgtgcc atgctgggca
    12181 ttgtctgccg agaagctgac cgaggtaccg tccggcatga tcgcttccag ctgctggacc
    12241 ctgacaatgc cggccggcaa caaccccatg tcgattttca ggctgcgcac accccatgaa
    12301 aaaggtgcgg ccagcaatgt gtgcaaggcc accagggagt cgagccgggc cgatgcctgc
    12361 tggaaatgct gcggcgacaa cagcatgccc tcatgccact ggatgcgctc ttcaaccggg
    12421 agggacttaa ccatggatgg ctccctcatc tgtcgtgcgc ccttccggga tacggagcgt
    12481 cttggcgttt ttcttcatag gaactcctcg gcttattcgg tcttcagggc tgacacggaa
    12541 aatgagcgcg cccccagctg cacgagtatt tccccctgca tctggtccac ccgtacgcga
    12601 tgctctccag gcgtcagata atccgcaaaa agcaggacgc caaccacccg agacgccccg
    12661 aaggtgtcgg cgggaagttt gagggtctgt ccgggcacga tctccatgct gcggtagctc
    12721 aacccctccg gaaaggtctt ggccagatcg gcgcgggacg aaaaccattt ggatgccggc
    12781 aatgtcgaca ccatgcccag cgttgcctcg tcccgaagca gcaccacgtc cagcgcaacc
    12841 ggggtattca ggttggcgcc ctcgctcgcg atgacagtca tgccgttcca gtccaggcgg
    12901 cttcctttcg ccgtgaagaa cccggcgaca ctgctgatgg tgctgcaggc cgacaagctc
    12961 cccaggacaa gaataatcag gcaaattttt agaaatttca tttttttctt aaccagggct
    13021 gttccctgca acggccatgg gccccgatgc atacggctga ggcaggcccg cgcccgtctt
    13081 tcgcatcccg acgccgatac ccgcaatcgt aatcaataac gccgccagca agacacaagc
    13141 cagcgccatt gcaacactgg cccgggaaaa aacaaagcgg acaggctcca gcgcgcgcag
    13201 gttcgatgat gcggcggttt caatacgctc cgaaggcagt ccgcgggaaa ccagcacgat
    13261 caggacacag gcaaacagca gcgtgagcag gttgcgcggg ccttcgcaga ccaggtcgat
    13321 cgccaggaaa agtgccagtg cagcttcaat gggaagattc agtatggaca gcaccacacc
    13381 ggcgaaccct accgcggcca ggctgttgtt gcccgccgat gcaaacgccg ccagcgaggc
    13441 accgacgcaa atcagcagga actcttgagg cgtgatggag cgcccataga tattggccac
    13501 aaacactgcc agcagcgaga aataaagtgc tgcaccggaa cgcatgaaca ccgacgcaac
    13561 cggaatcacc agttcgacaa tgccccggct gaaccccaac cgggaactca tggcctctat
    13621 cgtgtcggga atgcttgccg tggtgctggc ggaggtcagg ctgatgagaa caggcgcctt
    13681 cagcgccgac atcacactgg ctggagaaag ccctgacttt ttccagatca acagaatggc
    13741 cgctccagag agggaaaaag cgaacagata gaaggagccc agaaaactgc ccatcgcatg
    13801 aagcgtccgg gcgtcggtcc tggccacaaa ataggctgcc atgccaaaag ccagcaccgg
    13861 aataaaaacg ttgacgcggg aaatgatcaa ctcaagggtg cgataaatcg cctcgaaaat
    13921 gcccatcagt gcattggttt gcggcctgag cagcgcagca aaagccagcc cgaacaggat
    13981 ggcgcacagc aggatggcca gcgactggcc ttccgcaagc acccggaaaa agttgtccgg
    14041 caccgcagcc atccacaaat cgggcgccgc tgttacttcg gaagccccgg caccatacag
    14101 ggaaacttcg gcgtttgcgg catctccgcc ggcgttttgc accagggcac cgaggtattc
    14161 acggtcggca gagctcagat cctgaccggt gccggcgagc acgccgaaca ttgcccccag
    14221 cactgcacac agcaccacca ggcctaccgc cagacccaca atcatcaatg cgcgttttcc
    14281 aggttgcggc aaggccaatg tctgacgcag gccgaaaaaa gtggccacaa ccagcaacgg
    14341 aagcgcagcc atattgacca gggccaggta gatctgccca agaacaaagg cctcgccacc
    14401 cacggcaggt gcaagccagc ccagcagtgc tccccccatc acgcaaagca ggagagccaa
    14461 gggactgttc gacaggcgct tcaggagtcc catcccggcc tgttccagcc tcgtgcgagg
    14521 gcaaaagcaa cactggctcg cgccagttcc agtgagctgt cgatcaaccc caagcccgat
    14581 acatctgcat acctggaagc aatgggaatt tcggtgcagc ccatgatgat ggtggtcgca
    14641 ccttgccgcg cggcctcccg acaggcttcg gtcagatgtt gcccgccggc agccaggtcg
    14701 ccagccttga tttcgcgtat gcaaccgtcc acattgttct ggccatcctc gggatccggg
    14761 agcatgaagt cgatgccgcg cgaccgcagt tcccgctggt agaagcctga ggccaaagcg
    14821 ccccgtgtgg caaaaacggc cacccgcgag acacccgtgg gggaaattgc ctcgacgcag
    14881 gccttggcga tatgcagcaa cggcaccttg ctgctcgaac tcatttcatc aaaccagtga
    14941 tgcgacgaat tgcagggaat ggcgatgacg ccaacggcag cctggttaag tacttcgatg
    15001 cccgccagca gctgcggcaa gggatcccgc ccatgcccca aaatagcgct ggagcggtca
    15061 tgggtatgcg gcaagctcgc cacgatgacc ggcagatggt cctgatcgcg cgccgcaggg
    15121 gtgagcttga ccagcttgtc gagaaagtcg accgttgctg atggccccat tcctccaaat
    15181 acgccgattc gggacatcct ggcctcctat attttcaggg gcctggggca gatcagggac
    15241 accgcggcgt tgttgccaat caccagcacc agggtcctga gcatgtcgca aatcggatcc
    15301 accgccagga acaggatgaa agccgcctca aaaggaagac cgaggtagct gcatgtcagt
    15361 ccgaccagcg acaccgtgag caagcccgtc atgcccgccg acgcaaagcc ggcgagtatc
    15421 gatgccgaga ggatgacgat caactctgtc ggaccaatcg gttttgcata caactgggca
    15481 atgaacacgg tagcggccac gtaatagatc accggcccga tacgcagcag cgacacgctc
    15541 aggggcacca tcagctcgac ccttgaacgc gcaaaaccca ggcggtccgc cagactttcg
    15601 atcatgatgg gcatgcatgt cgcactgctg cgggtggcca acgcgagggc aaaggggccc
    15661 ctcaaggcat tgagagtctg gcccagcgtc gagttggaac gcttccagat aatgattgtt
    15721 gcaatgacca gggccagcac ggaagcaaca aaaaacgcca ggacaaactg ggccatggcc
    15781 tgcaaggggc cgatgccggt tttggccatc tgcgcagcgc tcatgcagaa aagaacgatg
    15841 ggcagcggat agttcaacca gcgcatcagg gtctggcagg agtggtaaat ggtttccagg
    15901 gactgggtca gcccgtcgga aacccgcgac ggaacctgac ccaccgcaaa accgaaaagc
    15961 aacgcgaaga cgagtgcctt gagcgtgtcg ccgctggcca gcgcggcaaa aatattcgat
    16021 ggcaccaagc tgaccagtac ttcggtgaag ctcaccgact tgaccg
//
LOCUS       DAIDFG010000035        15936 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_35_length_15936_cov_18.775770, whole genome shotgun sequence.
ACCESSION   DAIDFG010000035 DAIDFG010000000
VERSION     DAIDFG010000035.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 15936)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15936)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15936
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35_length_15936_cov_18.775770"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2980 a         5214 c         5134 g         2608 t
ORIGIN      
        1 caggccgtgc gcggctcacg caaaaaatcc ggtgctgcag cgcaggcccc cgccgatcag
       61 cgggttcgcg tgcagggtac cttgtcctcc aactcgggcg agctggtgcg cgactggtgt
      121 ttgcaggggc gcggcgtgat gctccgcagc ctgtgggacg tgcaggccca gctcgactcg
      181 ggcaagctgg tacggctgtt gccgcagttc gcgatgctgg acgccgacat tcagtggctg
      241 gcgcccttcc gcccgcagac accgaagcgg gtgcgtttgc tgattgactt cctggcggaa
      301 aaattccggc acgagccctg gcgcctgccc gcctgaaaac tctcagcctc tcaggcggcc
      361 cgggtcccgt tgcgcaccac cagccagacc ccgagcgccg tcagcgccgt ccccagcacc
      421 gtgaccacgg tgatggactc gccgaacagc aaccaggcca tcagcgcggt ggtgggcggc
      481 accaggtaca gcaggctggt cacggccgtg gccgcgccgc gctggatcag catgtagagc
      541 agcgagctgc cgcccagcgt gagcgcaagc accgaccagg ccagcgcccc catgaactcc
      601 gaggtcaagc gcacaggctc tgcctcgaac caggcgaaag gcagtgtcac cagcagcgcc
      661 gcgccgagct ggatggcgtt ggcgctgcgc acatcggccg cctggacaaa acgcttctgg
      721 tacagcgtgc cgatggtgat gctgagcagc gcaaaaacag cgcaagccag ggtgtaggtg
      781 gtggcctcgt tgcccgcgcc aaacttgcgc gagaccacca gcagcagacc ggccagcccg
      841 agcaccaggc ccagccactg gcgggcgctg acacggttgc cgctctggct ggcatcgccc
      901 atgcgcgaga cccacaccgc cgtcaacacc ggctgcaggc cgacgatcag tgccgacagg
      961 cccgaaccca tgccgccctt gacggccgac cagacaccgc cgaggtaacc tgcatgaatc
     1021 aggatgccgg tgacaccgag atgcagccac tgcgagcggc tgccgggcca ggccacacgg
     1081 gcgatggcga tccagaccag gaagcaggcc agcgacaaca cgtaacggat ggccagaaag
     1141 gtcaggggcg gcgcataagg caggccgtat ttggcgacga tgaagccggt gctccagatc
     1201 agcacaaaca cggccggcat ggcccggatc aaggcgctgt tcgagccggg cgaactcatt
     1261 tggcgcgcgc gcggatttcg ggaatggctt ttttcaggta gtaggccatg gaccagaccg
     1321 tcaggatggc ggacagccag atcagccagg tgccccagac gttggtgttg atgacgccga
     1381 acaggatgcc gtcaaacagc aggaagggaa tcgcgatcat ctgcaccgtg gtcttgatct
     1441 tgccgagcat gtgcaccgcc acgcttttgg tcgcgcctat cagtgccatc cattcgcgca
     1501 gggcggaaat ggcgatctcg cgcccgatga tgatgagcgc cacaaacaca tcggcacgcc
     1561 gcagatgcac cagcaccagc aatgcggcac acacgaggaa tttgtcggcc accggatcga
     1621 gaaaggcgcc aaaggacgag gtctggttga gtttgcgtgc cagaaaaccg tcgagccagt
     1681 cggtggccgc aaacaccacg aacatgacgg tggcaatcag gttctggctg gccggcgtca
     1741 tgtcgagata gaaaatgccc acgatcagcg gaatcgccac gatgcgcgtc caggtcatca
     1801 cggtgggtat ggtcagaaac atggcctgat tttgacatgc gcggcgccgg accgaacggg
     1861 cgggccatca gcacccggca aataccgccg ctttgcccca ctttcacacc gcttcgtggc
     1921 gcaaatacgt ctcaggccac gatccgggtt cagtgcagcg cgcggtagat ttcttcggcg
     1981 agctcgcggg aaatgccctc caccgtggca atgtcctcgg cgctggccga actcacgccg
     2041 cgtatgccgc cgaaacgctg cagcaggttg gcgcgctttt tggggccaat gccggcgatg
     2101 tcttcgaggc ggctggcacc cacgcgcacc ttggcgcgtc tggcgcgcat gccggtgatg
     2161 gcaaaacgat gggcctcgtc gcggatctgg gcaatcagca tcagtgccgc cgagtcgcga
     2221 cccagataga ccttctcgcg gccgtcggca aaaaccagct cctcgagccc aaccttgcgc
     2281 ccctcgcctt tctcgacgcc ggcgatcagc ccgaggtcca ggccaagctc ctcaaacacc
     2341 tcgcgcgcca tcgacacctg gcccttgccg ccgtcgatca gcaccaggtc cggcagccgg
     2401 gccgtgttgt tgcgcgccgc ttcggcgagc ttgctgtagc ggcgcatcag cacctggcgc
     2461 atggcggcgt agtcgtcgcc cggcgtgatg ccctcgatgt tgtagcggcg gtattcggca
     2521 ctttgcatct tgtggccatg gaacaccaca cacgatgcct gggtcgattc accggccgtg
     2581 tgcgaaatgt cgaaacactc gatgcgcagc acatcgagct tgtcgagcgg caggtccagc
     2641 gcctcggcca gcgcgcgcgt gcgcgcctgc tgcgagccct gctcggccag cagccgggcc
     2701 agttgcaggt cggcattggt ctgcgccatc tcgagccaga tgcggcgctg ctcgcgcggc
     2761 tggtggatgg ccgtgacctt gttgccggtc tgcatgatca gcgcggcgat cagttccttg
     2821 tccaccggtt cgctgcaaac cagcaccggc ggcaccggca cgtccacata gtgctgggcg
     2881 atgaaggctt ccagcaccag ggcttcgagt tttttgacct tgccgggcaa gacgccctga
     2941 gcgccctcac cgcctgccgc gccgccgtcc acatcgtcct cctcgagccc ggccaggtcg
     3001 gccgcgtgtt cgacatgggt ggggaaatac ggccggtcgc cgaggtgacg gccgccgcgc
     3061 accatggcca ggttcacaca ggccttgccg cccagcacct tgaccgccag gatgtccacg
     3121 tccttgtcgc caccgatttc catggactgc tggtgcagca ccttggacag agccgagatc
     3181 tggttgcgca gttcggccgc ctgctcgaac tccagctttt cggcgcggct ccgcatctgc
     3241 ttttccagct cggtcaggat gtccccggcc tggccctgca ggaaacgctc ggcgttggcc
     3301 acgtccagcg cgtactgctc gggcgatacg tggcccacgc agggtgcgga gcagcgcttg
     3361 atctggtaca gcaggcaggg ccgggttcgg ttgttgaaca ccgtgtcttc gcaggtgcgc
     3421 agcttgaata ccttctgcag cagcaaaatg gtttccttga ccgcccaggc gctggggtaa
     3481 ggcccgaagt agcggtgttt cttctccacc gcgccgcggt agtaggccac gcgcggaaag
     3541 tcgtgcgacg cgagcttgag gtaggggtaa ctcttgtcgt cgcggaacag gatgttgtac
     3601 ttcgggttca gcgtcttgat caggttgttt tcaagcagca gcgcctcggc ttcggagcgc
     3661 accacggtgg tttccatgcg gacgatcttg gtcaccatgt ggccgatacg cgtgccgccg
     3721 tggtttttct ggaagtaggt ggagacccgt ttcttcaggt cgcgcgcctt gccgacatac
     3781 agcaccgcac cctccgcatc gaagtagcgg tagacgccgg gcatggccgg cagcgccgcc
     3841 acttcactga gcagctgggg atcaaattcg tgcgatgggg tcatggtggt gggtgcgcgt
     3901 gtttggccgg cgtgggccgt catgcggaag aagtatcggc atttctggct ggcagccccc
     3961 gtattgtgga caatcccgat atggatcatc gcccgcaatg ggacattttt tgcaaagtca
     4021 tcgacaacca cggtgacatc ggcgtctgct ggcggctctg cgcgcaactt gcggcgcgcg
     4081 ggcagcgcgt gcggctgtgg gtggacgatg catccgccct gcactggatg gcacccgaag
     4141 gctgcgacgg cgtcaccgtc ctgccctggc tggaaccgct gggcatgaac atcctgcgcc
     4201 tggaagccgc cccctgcgag gtgctggtgg aggccttcgg ctgcgaaata gctcctgaat
     4261 tcatagctgc ctgcgcagac agggaaaggg ctacaggcct gaaacctgta tggatcaacc
     4321 tcgaatacct gtccgccgag ccctgggtgg agcgctgcca tgccctgccc tcaccggtgc
     4381 agcgcggccc ggccgccggc tggaccaaat ggtttttcta ccccggtttc agcgccgcca
     4441 ccggtgggct gctgcgcgag tccgcgctgg cggcgcgcca ggcggccttt gaccgcaaag
     4501 cctggctggc cgggcgcggc atggcgtgga acggcgaggt gctggtgtcg ctgttttgtt
     4561 atgagccccc tgccctggcg cagctgctgg accacctggt gcaggcgggc acgaccgcca
     4621 cgccggtgca cctgctcgtg accgcgggcc gcgccacagc cgccgtgcag gccgccgttc
     4681 aagataaaaa gtggccacag cccaacaagg acgggcgcga gccgctatca atttcatacc
     4741 tgccctggct gcgccaggac gacttcgatc acctgctctg ggcctgtgac ctgaactttg
     4801 tacgcggcga agattcggtg gtgcgcgcgc tctgggccgg ccaggccttt gtctggcaga
     4861 tttatcccca ggccgaccag gcccaccagg ccaagctgca agcctttctc gacatgctgc
     4921 aggcgccgcc ctcgctgcgg cgttttcatg aagtgtggaa cggcctgcag ggccaattgc
     4981 cggaacccga gctgccgctg tggaccggct gcgcccgcgc ggcccggcag cgcctgcagc
     5041 agcaacccga cctggtcacc cagttgctgg cctttgtcat gcagaaacag ggggcgacgc
     5101 catgaggctg ctgctggtgg aagacgacac catgattggc cgggccatgc gccagggcct
     5161 ggccgacgcc gggttcagtg tggactgggt catggagggc cacgcggccg agcgcgcgct
     5221 ggccaacggg gtttacgacc tggccgtgct ggacctcggc ctgccgggcc aggacggcat
     5281 ggcggtgctc aagggcctgc gcacgcgcgg caacgccgtg ccggtgttgg tcgtgacggc
     5341 gcgcgacgcc gtgtccgacc gcatcgccgg gctcaatgcg ggcgccgacg actacgtgct
     5401 caagcccttc gacctggacg aactgatcgc gcgcatacgt gcgctgctgc gccgccatgc
     5461 cggcggcggc tccccgctgc tgcaatgcgg cgagctgata ctcgaccctg tccgcaaaca
     5521 agtcagcctg aaaggtgaaa acgtgaacct gtccgcccgc gaattcgcgc tgctggagac
     5581 cctgatgcag cgccccggcg ccgtgttgtc gcgggcccag ctggaagact ccatctacgg
     5641 ctgggccgag gaaatcggca gcaacgcggt cgaggtctac ctgcacaaac tgcgccgcaa
     5701 actcggcgcc gacaccatcc gcaatgtgcg cggtgtcggg tacaaggtgg tggcgccatg
     5761 atgacccgac cggttcaatc catccagcgc cgcgtcctgg tctgggccat gggtgcgctg
     5821 gtggtaggcg cgagcttgct gataggcagt gcctactgga cgttgacgca cgagatggga
     5881 gaagtttttg aggacaacct caagcaggtc gcgctggcgg tggccaacca ccatggcacc
     5941 tacggcatgg cgcgcagccc gagcctggcg gagcagttgc cccgggtcta tgaggagtac
     6001 ggcaaattcg agttcgtcac cgcggcctgg acacgcagcg gcaccttgct gcacgcatcc
     6061 gacccctctg cggcgctgcc cttcctctcg cgcagcgggt tgagcgtggt cagcatcggc
     6121 catgagaaat ggcatcttta caccatcgtg ctcgacgacg gcattgtcca ggccgcccag
     6181 cgcgacagcg aacgcgaggc actggccaag gaaaccacca ccaagctgat cctgccggcg
     6241 ctgctgatgc tggctctgct ggcgatcctg ctgacgctgg cgctgcgccg cggcctgtcg
     6301 ccgctggcgc tggcggccag cgaggtcacg acgcgcagcg tcgaagcctt gcaccccatc
     6361 gcactgtcgg cccacccgcc ggaattgcac ctgctgatag gggcgatcaa cgacctgatg
     6421 gcccggctgg gtggtgcgct gtcgctgcaa aggctgttcc tggccgacgc cgcccacgaa
     6481 ctgcgtacgc cgatcacggc gctgcggctg caactgcagt tacttgagcg cgccagcgac
     6541 gaggcccagc gcgaggcggc actggtgcaa ctgcgcgccg gcatcgaccg ggcccagcat
     6601 ctggtcgagc agttgctgca gttgtcgcgg ctggcgcccg agacgccggc cctgcgccgg
     6661 gagccggtgg atctggcaga actggcccgg gccaccgtgg ggcgtttcag cgcgcgcgcc
     6721 gacgacagga aaatcgacct gggcgccgtg accgagggtt cgccaaccgt ccacgccgat
     6781 gtgcagcagc tggccatcct gctgaacaac ctggtcgaca acgcactgcg ccacacgccc
     6841 gcaggcggtc gcatcgacgt ggctgcgcag gtcgacaacg gccggcccag cctgagcgtg
     6901 accgacagcg gccccggcat cagcgccgcg gaacgggagc gggtgttcga ccgcttctac
     6961 cgcggcagca acgtcagtgc cgacggccat gcgacgcatg gcagcggcct cggactggcc
     7021 attgccaggg cggtggccga gcgccacggc accgacatca cgctggacga agcgcccggc
     7081 ggcaccggct tgcgggtcag tgtgcgcttt ccagcccgtt gacggccacc accggtgccg
     7141 gcaccgggcg acacggcgca aacaggtcca gcgacttgtc ataggacagc gtctggacat
     7201 cgagcacacc cagcagggag tgaaacaggt tgtcgtggcc gacctcggcg cccgcattct
     7261 tgcgcaggca ttcctggtcg atgttgaagc tttgacgaaa cccgggtgac agccacatca
     7321 ccagcggcac gcgtttctgg acctcgggcg cgatcgggta aggcatgcca tgcaggtaca
     7381 ggccgccgtc ccccagcgac tcgccgtgat ccgacacata caccatggcc gtgtcgtaat
     7441 gctgctgcgc acccgcgagg aaatcaatca ccctggaaac aaagaagtcg gtgtagagca
     7501 gcgagttgtc ataggcattg acaatttccg cggccgtgca ggcgctcagg tcctcgttct
     7561 ggcaagccgg ggcaaactgc ctgaacaccg ccggataacg cttgaagtaa gccggcccgt
     7621 gactgccgag ctggtgcatg acgaccagca ggttgccctg ggtgtcgcgc gcaatgcctt
     7681 ccatgccctg cagcagcacc tcgtccaggc attgcccttc ggcgcagagg gcctccacct
     7741 tcgcatggtc cagttgctcg gcgggcagtc cctcgcaaac gcccttgcag cccgactggt
     7801 tgtcgcgcca gagcaccttg aaaccggcgc gggccagcac atgcagcacc gactcatggg
     7861 accgtatctt cccctcgtcg taatgaatcc ggccgtaagg ggaaaacatg cagggcaatg
     7921 acacttcggt cgaggtgccg caggcggtcg catgcgggaa gctgatcacg tcctgccggg
     7981 ccagcaaggg gttggtctgg cgcgcataac cgttgagcga gaaattctgc gcgcgcgccg
     8041 tctcgccaac gatcagcacg aacatcaccg gccggggccg gccgctccag ctggcgccca
     8101 gccgggcatc ctcgccgacc ggcagtttgg gctgcgacgg ccgctgcacg ccgcccagcg
     8161 cattgccggc cagggcggcc accacattgc ccggggtcag cagataacgc acctccttgt
     8221 ggttgcgcat cagcgaggca aagtcggcaa acaccagcag caggctggcc gcgcccaccg
     8281 ccagcgcggc cgcaatccag cccaggcgga cgaccagcgc gcggcccagg ctgcgccgct
     8341 tcaactgcgg ccaccagacc agcgccgccg gcagcgcgcc cagcagcaca acgcgcggta
     8401 ccatgcccca gaccagcagt tccctggctt cgttgacatt ggtggccagc acattgcgca
     8461 gcatcgcgcg gtccatgtag atggcgtaat ggtccatgta ccacgcggcg aaagccgtgc
     8521 acagcaccag cagactcagc agcggcctga cggtcaggcg tgtcgcgagc agtgccagga
     8581 aggcgaagta gaacgccgaa aaggcagtaa acagggcagt tgcaaaaccc caggtcgcaa
     8641 gcagttccca gccccggccg gccagcgcgg cgcgccagaa cggcccgttg ccgctgagca
     8701 gcagaaagcc gacgcacagc aacaccagcg tttcgacctg cagggacggt cgctgcaacc
     8761 acgatttgcg gggcaaggcg gaggccggca ccagggcgcc ggtggaagaa acggtcatca
     8821 tcggggttgg tgggtgaccg ggcggcgggc accctggagt cagggaagcc tggagcttgc
     8881 agccttgacc cttaacccgc ccttaaacca gctgctgcac ggcaggggcg gcccggaacg
     8941 gccgcggcgc ttttgggtgg ctgccggaaa aaaacgttaa aatacagggc tttgcatggc
     9001 ccggcatcgg gccgatgccg cactcccgcc gcagacccgc atcaccccag acgcatttgg
     9061 cggccaacac tttactagaa actgttatga aaatcgctca agaaatacgc gctggcaatg
     9121 tcatcatgca cggcaaggac cccatggtcg tcctcaagac cgaatacagc cgcggcggcc
     9181 gcaactcggc caccgtgcgc atgaagctca aaagcctgat cgccaacttc aacaccgagc
     9241 aggtgttcaa ggccgacgac aagatcgacc aggtcatcct ggacaagaag gactgcacct
     9301 actcctactt cgccgacccg atgtacatct gcatggacgc cgactacaac caatacgaag
     9361 tcgaagccga aaacatgggc gactcgctga actacctgca ggacggcatg gaactcgaag
     9421 tcgtgtttta cgacggcaag gccatctcgg tcgaactccc caccagcgtc aaccgcgaaa
     9481 tcacctggac cgagcctgcc gtcaagggcg acacgtccgg caaagtgctc aagccggcca
     9541 aactggccac cggctttgaa atcggcgtgc cgatttttgt ggcgcaaggc gatgtggttg
     9601 aaatcgacac gcgtaccggc gaataccgca aacgcgtctg acctgcccct tcccggcatc
     9661 ctggaaaaaa ggctcctgac ggagcctttt tgcatcccga aaactttcag agcgacgtat
     9721 ggaacaaaca gacgacttat ccggcagccg acttgcagac gcatgggcgg aactgcaggc
     9781 ccactccgac aggggcatcc cgctggaggc caatgcctac cggctggcct tcgccgaccc
     9841 cgagttcctg ctgcggcgcg agacccgcgg catccgtttc cagctggaga tgctcaagcc
     9901 ggacatggag caacaggccc agggcattga aaacaccatc gtggtatttg gcagtgcgcg
     9961 tttcccggcg ccggaacagg ccgagcaggc actcaaactc gcccacgcct ccggtgacgc
    10021 gcaggccctg gcactggccg aacggcaggt gcgcaacgcc gcctactacg accaggcccg
    10081 cctgttctcg cgcctggtcg gcgcccacag caaaacccgg tcacccgaag agcagctgtt
    10141 catctgcacc gggggcggtc ccggcatcat ggaagcggcc aaccggggcg cgcaggaaat
    10201 gggcgtgccg acggtgggcc tgaacatcgc gctgccgcac gagcagaact ccaaccccta
    10261 cgtgacgccg tcgctgagct tcaagttcca ctacttcgcg ctgcgcaaga tgcacttcat
    10321 gatgcgcgcc aaggcgctgg tggcgtttcc gggcggtttt ggcacgctcg acgagctgtt
    10381 cgaggtcatc acgctggtgc agacgcgcaa ggccaaaccg gtgcccatca tcctgttcgg
    10441 ctcggagtac tggcaacgcc tgatcaactt cgacgtgatg atcgatgaag gcgcgatttc
    10501 acccgacgac ctcaagctct tccagtacgt cgatgacccg caggtggctt gggacgcgat
    10561 caggactttt tatcagctgt agccggccgc agcagccagg cggcgcggct gcccgcgccc
    10621 accaccgcgc cggtagccca gaacacgccg gcgatgccga tcacggcgcc cagggtgccg
    10681 aacagcatgg gcatgaccac actcgaggcg ttgatcgcca tcaggcgcag gcccaatgcc
    10741 tcgccgtgcc tggcctcggg tgtgatctgg tgcagcatgc tcatgatcat gggttgcacc
    10801 gtgccgagcg ccaaacccag cagcactgaa cacaggccca tgcccagcgc cgtctgcatg
    10861 aaggggtaga cagcgaacag caccgcggtc accagcatgg cgaccgccag cacccgccac
    10921 tcgcacaggt gcgccgccac cagcggcatc accatgcgga tggcggctgc ggccacggca
    10981 aaggcaccca ggatggaacc gatcaccgag gcgctgatgc ctcgttcaaa gcccagcacc
    11041 ggcacgacaa aggtgtgcac gtcccagcag gaagacagaa accagttgac cagcatcagg
    11101 cggcgaaagg tggtgtcgcg cagcaggtcc caggcgctgc cctgcctggc gccgtgcgcg
    11161 gcgatcacgg gcggcagctc gcgcgtcttg cgcaccagaa accagctggc cagcggcagc
    11221 accgccatca ggaaaaaagc ggcacggtag cctgaggtgc tgttgggcat ggcaccggca
    11281 tggtcgatca gcagaccggc cgagaacggg ccgacaaaat tggaaatcgc cgggccgatc
    11341 gacagccagc tgaacacctg ccgcaactgc aaacctcccg tcgcggcccg gccgacgtgg
    11401 cgctgcagcg aaatcacggc ggcgcccgtg gccccgccgg tgagcagcgc cgccaggcag
    11461 agcaccggaa acaccgggaa tgccaccgcc accccggccc ccagcacggc cgccgcgacg
    11521 ctgtacccca tgggccggcg cagaccgtgc cggtcggcaa aacgtccggc gggcagtgcc
    11581 agaaacacct gggtcagggc aaacaaggcc agcaacacgc cgaccgcggc cgggctgtaa
    11641 ccctcgcgca gcgccagcaa gggcgcggcc atgcgggtgc cggccatgca ggcgtgcagg
    11701 cagatctggg ccaggatcag gcgggcaagt tcaggtgtca tgcgtcgcca ggatggcgct
    11761 caaccctcgt ccgggtcctt gtccatggga atcagccgcg ccgagtcaag ctctggcaag
    11821 gcctcgacgt tgcgcagggc cttgcccatg acccggctac gctgctccgc actgctcagc
    11881 gtattgagca cggtttcagt ctgcgacttg accttggcca gcacgtcgcc gaacttgccg
    11941 aattcggtct tgacggcacc caggacctgc cagacttcgc tggaacgctt ttcgagtgcc
    12001 agggtccgaa agcccatctg cagcgagttc agcatcgcca gcaaggtggt cgggccggcc
    12061 agcgtgacgc ggtgctcgcg ctgcagcacc tccatcaggc cgggccggcg caggacttcc
    12121 gcatacaggc cctcggtcgg caaaaacagg atggcaaagt ccgtcgtgta cggcggctcg
    12181 atgtattttt ccgagatcga cctggcctcc atgcggatgc gcacttccag cgccttgccc
    12241 gccacttcgg cgccaagcac gtcggcgcgc tgctgggcat cgagcaggcg ctcgtagtct
    12301 tcgttgggaa acttcgcgtc gatcggcagc cagaccggat cgccattgtc cgaacggccc
    12361 ggcagccgga tcgcaaagtc caccgggttc ttgccgccgc ggcgcgtgat gacttgcgcg
    12421 gcgtactggt cgaccgtgaa cacctgctcg agcagcgccg agagctgggc ctcgccaaaa
    12481 atgccgcgcg ccttgacgtt ggtcagcaga tgcttgaggt cgcccacgcc ctgggccagc
    12541 gtctgcattt cgcccaggcc cttgtgcacc tgctcgagcc ggtcggcgac ctgtttgaaa
    12601 ctttcgccca ggcgggtttc cagcgtggtc tggagctttt catccaccgt ctggcgcatc
    12661 tcgtcaagct tggccgagtt ggtctgctgc agttgcgcga gctgtttctc cagcgtttcc
    12721 cggacttcgc ccatacgccg cgcattcgat tcgctgaccg actggagctg cgtggtcagc
    12781 gtgtcggaca gggttttctg cagcagcgtc aattgctggg aaaaagcgtc gatctgcgca
    12841 ttctgcgtgc ggatggcctc ggcgctttgc tgcaccaggg tttgctggaa ggtggcaaaa
    12901 ctcgtcgcca gttcctggcg gctggcgcgc gagctgtcgc cgatttcccg ccgcagctca
    12961 cgctcgagct tgtcgttgcc ggaggccagg ccggcgagca gctcttcgcg gccactctcg
    13021 gccggcttgc gcagcaacag ccagagcaga aggagcagga caagcagcag cgctgcgaag
    13081 accagccagg cttgcaaggg gttcatcggt gctatttatt tcgcagcaag gaccgcagga
    13141 ttcaagggcg tcagcggcct tttttccttg aagaacccga tcaggttgtc agccgccagc
    13201 atggccatgg ccatgcgggt gggcaccgtg gcactggcaa tgtgaggcgt cagcacgacg
    13261 ttgggcaccg tcaacaggtc gggatgcacc tggggttcgc cttcaaacac atcgaggccc
    13321 gccgcggcta tacgcctgtc gcgcagcgcc gcagccagcg cggcatcgtc cacaatgccg
    13381 ccgcgcgcta tgttgaccag ggtcgccgtc ggtttcatca gcgccagctc ggcagcgcct
    13441 atcgtgtggt gcgaggcggg cgaatacggc aacaccagca ccacatggtc ggcggtccgg
    13501 agcagttctt ccttgctgac gtaactcgcc ttgcactcgg cttccagcgc agcgtccagg
    13561 cgcgagcggt tgtggtaaat caccttcatg ccgaagccgt gcgcaccgcg cctggcaatg
    13621 ccctgcccga tgcggcccat gcccagtacg cccagggtgc tgccgtggat gtcggagccg
    13681 gcaaacatgt cgtagctcca cttgctccac ttgccggccc gcaggtaatg ttcgctctcg
    13741 gtgatgcgtc gggccgtggc catcagcagc gcaaaaccga aatcggcggt ggtttcggtc
    13801 agcacatcgg gtgcgttggt ggccagtaca ccggccgccg tcatggcatc gatgtcgaag
    13861 ttgttgtaac ccacggccat gttggcgcag atcttcagtg tcggacaggc cgccaacacc
    13921 tcggcgtcga tgcgatcgcc accggtggtg aaggcaccat ccatgccccg cagtttttcg
    13981 atcagctcgg ctttggacca ggtttcgtct gcctggttgg catgcacctc gaagtgctgc
    14041 tgcagatgcg tgatgacttc aggaaagacg gcgcgggcga cgaggatgtt gggtttgctc
    14101 atgggacttc ttcaatgcgt tgaaaggaat ttaccggaac cagatgaagg tcatgacgat
    14161 gaatagcgga atcagcaccg cgcccgacca ggccatgtag ccgaagaaac tcggcatctt
    14221 catgccacgg tcctcggcaa tggccttgac catcaggttg ggcgcgttgc cgatataggt
    14281 gttggcgccc atgaacaccg cgccggccga gatggcggcc agcgtcggcg ccagcgtggt
    14341 catcagggtg gcgggatccc caccggccgt gttgaaaaat accaggtagg tcggcgcgtt
    14401 gtcgaggaag gaactcagcg cgccggtggc ccagaaatac atggccgggt ccggctggcc
    14461 gtcgggccgg gtgacagccg acactaccgc gccaaagggt ccggcggtcc cggccttgag
    14521 catcgcaatg accgggatga tggtcaggaa aatgcccgca aacagcttgg caacctcggc
    14581 catggggccc caggaaaact ggttgtcctc gtgcaccttg tcggccgtca tcttcagcga
    14641 cagcaaggtg atggcgatca ggccggcgtc acgtaccagg ccgggcaggc cgacctcggt
    14701 gccgaaaaca tcgaacacca ccgacgactt ccagaaaccg ctgagcagca ccaggccggc
    14761 gacgccggcc agcagcgcga aattgaaggc gccgtcgaaa cccaggcggc cggtatccgg
    14821 cgtcgggtcc accggctgca cgccctcccg gcggtagtac cagctgtcca gcaggtaaaa
    14881 aatcaccagc aggctgccga tcaggaacag ggtctccggg aaaatgtgct tgaccgtcca
    14941 gaagaagtcg acgcctttga ggaagcccag aaacagcggc ggatccccca gcggcgtcag
    15001 cgaaccgccg gcattggaga cgatgaagat gaagaacacc acgatgtggg cggcgtgttt
    15061 gcggttgtca ttggcgcgga tcagcggacg gatcatcagc atggaggcgc cggtggttcc
    15121 catgaagctc gccagcacgg cgccgatcag cagcaggccg gcattgagcc cggggctgcc
    15181 atgcaggttg ccgcgtatga agatgccgcc ggccaccgtg aacagcgccg tcagcaggat
    15241 gatgaaggga atgtattcgg caaccagcgc gtgcacgagg ctggtgcccg ccagtgccgg
    15301 gccgaacagc acggcaaacg gcaggaaaaa cgccagggtc caggccgctg acaccttgcc
    15361 gaaatggtgg tgccagaacg agggaagcag cagcggcatc aaggcgatcg acaacagcag
    15421 ccccgcaaac ggaatacccc agagcggcga gagctggctg ccatcaaagc cggccgccgt
    15481 ggcccagcca ggcagaaagg acaacaaggc cgcgagcgtg atccagcgaa attttttcat
    15541 gaggtctcct gtcgttttta tgtaaggcag ggcagttgcc tcctgcctct tattgtccgt
    15601 gtcatgcaat gtcaaaaacc tgacgcaggt aagccaggta tttctcgtca tcacacatgt
    15661 tttttgacgg cgtgtcggac agcttggcca ccggctgacc gttgcagcgc accatcttga
    15721 tgacaatttg cagcggttcg tagccgaggt cattggtcag gttggtgcct atgccgaaag
    15781 caagctgaca gcggtccctg aactgctggt agagctcaat ggtgcgcggc accgtcagcg
    15841 catcgctgaa aatcagcgtt ttggtccggg ggtccacccg gttcttgcgg tagtgctcga
    15901 tcaggcgctc gccccaggca aacgggtcgc cgctgt
//
LOCUS       DAIDFG010000036        15328 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_36_length_15328_cov_17.985726, whole genome shotgun sequence.
ACCESSION   DAIDFG010000036 DAIDFG010000000
VERSION     DAIDFG010000036.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 15328)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15328)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15328
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36_length_15328_cov_17.985726"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2612 a         4909 c         5100 g         2707 t
ORIGIN      
        1 cgcgccaacg gccagctgat cgatctggga ccgcgcgaat ggacggtgat ggagtatttg
       61 ctgattcacg cgcccaaacc tgccagcaag gaaaaactgc tcgccgcgct gaccggctgg
      121 gacaaggaaa tcacccccaa cgcgatcgag gtctacgtct cgcgcctgcg tggcaagctg
      181 gagccgcacg gcatcgcact gcggtccatt cgcggctttg gttaccggct ggagctggtg
      241 ggcggcgagt cgctggacgc gccggttggg tagactgccc tgatgcgtga gaacactggc
      301 gctgacccgg ccaaggcggc tgtcgcggaa aaagccgttg cgttgagggt gcctgcggcc
      361 atgaagtccg ggctgcagcg ccgcctgctg ctgctgctgc tggctcccct gagcctgttt
      421 gccttggtga gcatttactt cgactacgag gcggcgggca atgccgccct gcagcaggac
      481 cagcaactgc ggcgcctggc gcccctgctg gccgactcgg tgctcgcgtc cggccgcacg
      541 gccgacgaca gcccggtgat gttgctggcg ccgccggtcg aggagttcat caaggcgcgc
      601 accgggtttg ccggttaccg ggtgtcggat gccgagggcc gttttctgac cggcgatgcc
      661 tggatcagca tggtggtgcc caccacccgc gagcccgagt ttcacagcat ggaggaaaac
      721 ggcgtcacct accgggtggt ggcgcaacgt gtcagaaccg cggcgggtga gctgatcctg
      781 cagctggccg atggttcgga cgcccgccag cagtgggtgc gctcggtggt tttcaaggtg
      841 ttgctgccca acctgattct ggtgctggcc gctgccctgg ccgtcaactg ggccgtcacc
      901 cgggccttgc ggccgttgct ggcgctcaag gaagcggttg agcgccggtc tccgcgtgac
      961 ctcagtgcca tcgacccgca gagcacgccg ggcgaagtgc ggccgctggt ggcctcgctg
     1021 aaccgcctgt ttgcgctggt caacgaccag gccgagggtc agcgccgctt tgtggccgat
     1081 gctgcgcacc agctgcgcac cccgctggcg gggctgcagg cccaagtgga ggcctgggcg
     1141 caggcggcca tggcgcccgc ggcaggcagg caactgaccc tgtcggccga caagatcatg
     1201 gcgctgcgca gcgccacccg gcgcacctcg cagctggcca accagctgct ggccctttcg
     1261 cgggccgacg cctcccacat ggcgggccag ccggtgcatc gggtggacct caaggacctg
     1321 tgcgagtcgg tgctggccct gcacctggac gcggcctctg ccaaaggcat cgacttgggg
     1381 ctggaggtgt attttgccca ggtctccggc cacgagtggc tgctgcgcga actgctcggc
     1441 aacctggtgg acaacgctgt ccgctacacg gcgccgggcg gcacggtcac gatacgctgc
     1501 ggcccgctgg aggcgcaggg cgctgcgggc gaggcactgg tttttctcga ggtggaagac
     1561 gatgggccgg gcatccccgc cgccgagcgc gcccgggtgc tggaacggtt ttaccgggtg
     1621 cccggcacgg gcggggaggg caacgggctg gggctggcga ttgccgacga gatcgcgcgc
     1681 gtgcaccggg cgcagctgat gctggaggcc gccggcagcc gggatggcgt cgcctcgggc
     1741 ctgcgggtgc ggctggtcat gcgttccccg gcctgagagg ccgggcaaaa accgcgccgc
     1801 ggcagggcct ggcacacggg tttgcccctg accggcgtcg tccgaaagcc ccctctggct
     1861 gtgcgagaat ccaaagcgtc ggcccagttt ttgaaacatc tcttacaaca acgcactgca
     1921 tgccctatgt ccgagtttga gtctgatgtg agtcccgagc tctccacgcc ggctgcgccg
     1981 cgcaagcgcc ccaagccggg cgagcgccgc gtgcagatcc tgcaggcgct ggcgaccatg
     2041 ctggagcagc ccggttcgga gcgcatcacc acctcggccc tggcggcccg gctcgaggtc
     2101 agcgaggcgg cgctgtaccg ccactttgcc agcaaggccc agatgtttga gggcctgatc
     2161 gagttcatcg agcagaccgt gttcaccctg gtcaaccaga tcgccgaacg cgagaccgac
     2221 ccggcgcagc gtacccgcag gctggtcatc atgctgctgc agtttgccga aaaaaacccg
     2281 ggcatgacgc gcgtcatggc cggcgacgcc ctggtgttcg aaaacgaacg cctgcaggag
     2341 cgcatgaacc agtttttcga caagctcgag tccagcctca agcaaaacct gcgcgatgcg
     2401 gccggggccg acggcaccgc cacaccgacc gtccaggcgc aggtccaagc ttcggtcatt
     2461 gtggccttca tgatgggccg cctgcagcgc ttctcgcgtt caggcttcaa gcgcctgccg
     2521 accgagcacc tcgacgccag cctggccgtg gtgctggctt aactcacttc caaaaagaga
     2581 gcagcttgcg cccgccctct aagggctggc ggccgatttc gttcacaacg ctggggcacc
     2641 ggccaagggc tcaggcgccg tccagctcgg cctgcagctg cgccagcgcc gccagaaccc
     2701 cgagccggtg aatgcggtcc agctgccggg ccaccagtgc cagccattgc gtgttgcccg
     2761 gcagggcctc gccccagatc acccgcaatg tgcccagcgc ctgcacggtg gctgcggcat
     2821 cgccggcatg ggccaacgcc agcgcctgca gttcgccggc catcgggtcg ttcatcgcat
     2881 aaggtgttcc ctgctcatcg tggccgcgca cataacgcat ccagcccgct gcggcaaacg
     2941 cgaggcgatc cacaggcagg ccggcgcgca gcgcgcccag caccgagggc ggccagcgct
     3001 gcggaatttt ttgcgaggaa tcggtggcga tctggtgcac gctgtgttgc agcgcggggt
     3061 tggcaaaccg tgcaagcagg gcgtcccggt agcccgccca gtccgggcgt gccagttgcg
     3121 ggccgacttc ctgcgtcatc aggccgtgga tgaagcgccg caccgccggc tgtgtgatgc
     3181 attcgccgat caccggccag ccggccaccg cgcccagaca ggccatggcc gtgtggctgc
     3241 cgttgagcag gcgcagcttg gcgtcctcga acggggccac gtcgtccacc accgtcacgc
     3301 cggcggcggc cagcacctcg gcatcgcccg ggtcggcaaa gcgccgttcg attacccact
     3361 cccagaaggc ctcggtgccc agcgccgcct ggtctgccac gcccagcgcc tgcgcggcgg
     3421 cagccagccg ctgcggcgtg gccgcgggga cgatgcggtc caccatgctg tcgggaaaag
     3481 cgcagcggct gttgatccat tgcgcgagcg ccgggttgtc cacctgcgcc ttgttgacac
     3541 acagcgcctg cagctggcgc ccgttgtgcg ccaggttgtc gcaggacgcc agcgtcaggc
     3601 ccggcaggcc tgcggcgtgg cgcgccgcca gcccctgcac caacagttcg gctagtgccg
     3661 gcccgtagcc tttttccgtg acggtcagcg tgacccagcg cgtggcgggc gccgccagcg
     3721 cctgtaccac ccgggccggc tcgcgcgcgg ccacggcggt ctgccagatc gcgccgccga
     3781 cctgccattg gctgccggcg cggctggcca cctgcaccgc gtacaggccg tcctgcgcgc
     3841 tcaaggcatc ggccagttgc gtgctgcgca tggccacgcc gagcacgccc cagcgcgtgt
     3901 cgccgcgctg cagcagcgca tcaaaaacca gcgcctgatg cgcgcggtgg aaagcgccca
     3961 gcccgaggtg cacgacgccg ggcgtgccct ggcgcgggta ggtcgggacc gggatgccgc
     4021 gcgtagcgag gacgggcagg caggcgctgc ccaaccgggg cgccgcttca gcggacttca
     4081 tggtcatttt ggccttcagc ccaaaccaga taagcgcagg cagctatcaa tttcatagtt
     4141 tcaggtgggc ggcggtcgga aggcgtcagt cgggcagcga caccccgacc ttgcggcccg
     4201 cggccacgat ctcctgccag gtctgggcat cgaccgcgat gccacggtcc tgccgctgcg
     4261 cgcgataggc ccgctccggg tcaccggcga tctgcaccgc gtcaaatccc ggcgccactg
     4321 gcccggcctg gagccagtcg acaaaagcca gcgcttcctg ctcaaacgtg gcctggatcg
     4381 ctagcctggc cgggtcgatc aggatggtca acatgccgtt gatcaccgcg cgcacggccg
     4441 ggtcggtcgg cttgtgccag gtgccgctgc cggtcagcgc gccgcccagc agttcacagg
     4501 ccacggccat gccgtaaccc ttgtgttcgc caaaggccag cagcgcgccg aacaggccat
     4561 tgctctgcgg caccacgacc acgccggggt cggtggtggg ctggccgtgt tcgtcgatca
     4621 gcgtgccggg ctccacgcgc cggccttcgt tgtgggcgac gcgcattttt ccttgcgcca
     4681 cgcggctggt ggcgaagtcc aggacgaaag gtgcacgtcc cgccaacgga atgccgatgc
     4741 agcaggggtt ggtgccgtag cggccatcgg caccgccgaa aggtgcgacc accgggcgcg
     4801 acagcacgtt gacaaaatgc agcgacacca gcccctgggc cacggccatt tcggcgaagt
     4861 gcccgatgcg ccccaggtgg tgcgcgttgg ccaatgtcat gatgcagctg ccatgggttt
     4921 tggcgcgttc catgcccagc gccatggcct gctggccgac gatctggccg tagccgcgct
     4981 ggccgtccag cgtgagcagc gaaccggtgt cgagcaccac cttcacgccg gtgttgggat
     5041 gcaggccgcc ctcgagcacc gcgtccacat aacgcggcag catgcccacg ccgtgggagt
     5101 cgtggccgct caggttggcc agtaccaggt tggccgccac ggcagcggct tcatcggcag
     5161 tgctgccggc tgctattaaa acggtagcta cttgcgcttg cagtgattgg gctgggagtg
     5221 ttttttgcat ataaaaagta gctggatcac atcacggcgc cgacttgcca gggcacaaat
     5281 tcgttttgcc catagccgtg ttgctcgctt ttgctctgcg cgcccgaggc ggtggccagc
     5341 accatgtcga aaatccgctg gcccatttcc tgcacagaca caccgccgtc gatgatctcg
     5401 ccgcagttga tgtccatgtc ctcctcctgg cgctgccaca gcgccgagtt ggtggcgagc
     5461 ttgagcgaag gcgagggcgc gcagccatag gccgagccgc gtccggtggt gaagcagatc
     5521 aggttggcgc cgccggccac ctggccggtg gcgctgaccg ggtcgtaacc cggcgtgtcc
     5581 atgtagacaa agccgtgtgc gcccacgggt tcggcgtatt cgtacaccgc ctcgaggttg
     5641 ctggtaccgc ccttggctac ggcgcccagc gacttttcga gaatggtcgt cagaccaccg
     5701 gccttgttgc cgggcgaggt gttgttgttc atctcgcctt cgttgatggc ggtgtagtgc
     5761 tcccaccact tgatgcgggc gatcagtttt tcggcgatgt cggggcgcac ggcgcggcgt
     5821 gtcagcaggt gctcggcgcc gtaaatttca ggggtttcgc tgaggatggc ggtgccgcca
     5881 tgcgccacca gcaggtccac cgccgcgccc agcgccggat tcgcgctgat gcccgaataa
     5941 ccgtcggagc cgccgcattg caggccgatg gtgatgtggg ccgcgctgca gggttcgcgt
     6001 ttcaccgcat tggcgcgcgg cagcatctcc tggatcaggg caatcccctt gtccaccgtc
     6061 ttgcgcgtgc cgccggtgtc ctggatgttg aagacgcgga agttgtcgcc ttcgcgcagc
     6121 gcgctgtgcg ccagccaggc gttgatctgg ttggcctcgc agcccaggcc caccaccagc
     6181 acgccggcaa agttggcatg ggtggcgtag ccggccaacg tgcgctccag gatctgcata
     6241 cccatgccct cggtggccat gccgcagccg gtgccgtgcg tcagcgccac cacaccgtcc
     6301 acgttgggaa agtccttcag cgcggccggg ttggtttgcc gcgaaaaatg gtcggcgatg
     6361 gcacgcgcgg cagtggccga gcagttgacg ctggacagga tgccgatgta gttgcgcgtg
     6421 gccacccggc cgtcggcgcg cttgatgccc atgaaggtcg cttcgcgccg aggggcggcg
     6481 ggtttcacgt cggcgccaaa ggcatagtcg cgcgcaaagt cgcctttgtc gggacccatg
     6541 tcgaggttgt gcgtgtgcac atgctcaccg ggggcaatcg ccttgctggc aaaaccgatg
     6601 atctggttgt agcgccgcac aggctcgccg gccgccagtg tgcgtgtggc gaccttgtgg
     6661 ccgggcggga tcaggccctt gacggtgatg ttttcgacct gggtgccgcc caccagctgg
     6721 gcgcgggcaa tcaggacgtc gtcggcggga tgaaggcgga taaatggggt catggtgggt
     6781 tcggggcgat gggccgtctc ggtgaaaaat ttcagtaaac catttttggc agccagagca
     6841 ccagcttcgg aaaataggtg atgacggcca gtgagccaag caggggtacc agccagggca
     6901 ggatggcgat ggtggtgcgc tcgaccgaga gtttggccac ccgtgccagc acaaacagca
     6961 ccatgcccat gggtggatgc agcaggccta tcatcaggtt gagcaccatc accagaccga
     7021 agtgaaccag gtcgatgccc agctgctggc agatcggcac aagaatcggc accaggatgg
     7081 tgatggcggc ggtgggttcg agaaagcagc cgacaaacag catcagcagg ttggccagca
     7141 gcaggaacac ccagggctcc ttggtgaaac caagcaccca ctcggaaatc gccgcggtga
     7201 cgccggtggc ggtgagcatc cagccgaaga tgctggccgc cgccacgatg aacagcacgg
     7261 tggcggtggt ttccaccgtg tccaggcaga ccttgacgaa cattttccag ttcagcgtgc
     7321 ggtaccaggc caggcccagc accatggccc agaggcaggc ggcaatggcg ccttcggtgg
     7381 gcgtgaagat gccggtcgtc atgccgccta tcagcagcac cggcgtcatg atgggcagca
     7441 cggcctggaa gttgaagaat ttgtcggcgg caaacagcac gatcaggccg gcaaacacgg
     7501 tgatctgcgg cggaaagccc gccttggtga tcagcagcca gagcgccagc ggccagccgg
     7561 ccaccaccag ggtttcgacc agcgccttga tcacgcgggg ccactcgaac ttgatgtcgg
     7621 cgccccagcc gttcttgtgg gcgaagtaag ccacggtcag catcatcagc acgcccatca
     7681 ggatgcccgg caggatgccg gccagaaaca gcgccccgac gctgacgttg gccatcatcc
     7741 cgtaaatcac gaagggcagg ctgggcggaa tgatgggccc gagcgtggcc gatgctgcgg
     7801 tgacgccgac ggcaaactct ttgctgtagc cgtggtcggt catggccttg atctcgatgg
     7861 tgcccaggcc ggcggcgtcg gcaatcgcgg tgccgctcat gccggcgaac accaccgaac
     7921 ccaccacatt cacatggccc aggccgccct tgagccagcc gaccagcgcc agcgcgtagt
     7981 tgtagatgcg gttggtgatg ccggcgttgt tcatgaggtt gccggccagg atgaaaaacg
     8041 gaaccgccag caacggaaag ctgtcgatgc cgctgaccat gcggtggatc accacgaaag
     8101 ccggcagggt gcccgtccac cagatgtaaa ccagggctga acccgccatg gcgatggcca
     8161 ccggaatgcc gccactcatg agcagcagga aaatgatttt gagcatggtg tgtgggtgtt
     8221 atttgtcgtc catcgaggac tcgggacgtt ccaggacgga gtagccgcgc cggtggtgga
     8281 acagcgcaat ctggacggag cggaaggcca tggcggcgaa gcccagcagg caaatgccgt
     8341 agacccagtt catgggcagg tcgaccatgg tcatgcgcga attgctgccc agcttgagca
     8401 tcatcatcac cgtcagcacg gcggctgcgg cgaagaagcc cgcgcgcagc acatcgacgg
     8461 cggtggccat gacgcgtgcc atgggcgtgg gcatgaattt gtagaaaaaa tccacctgga
     8521 tgtggttgtt cttgaccacc cctatggtcg cgcctgtgaa gaccaccgcg atcagcaggt
     8581 agcgtgcgat ctcctctgtc caggcggccg agtcgttgag tacatagcgg gtgaagaact
     8641 ggtagaacac cgtggcgccc aggatccaga agaagcccag tgccacccag gcttccagtt
     8701 tggtgtcgga aaggtccacg gcttcgtcct gcgcgtgaaa ctgtccgtcc gcccccatca
     8761 cgggggccat gtcgtcaatg gtttccatct tgcgggttcc gatcgtcctg agcttgtcga
     8821 aaggccgggc tgccgatgtg gcggcaagcc cggcccgggg ggtgacttcg gtgacttact
     8881 tgatggcgat gatgcggtcc cagtctttct tgtcgagctt catggattcg aaggtgatgg
     8941 ccttgagtac gcgctgctgg aagtcggcgc ggtcaaccgt caccacgttg ttgccctttt
     9001 tcttgaactc ttccaccagc tcgccttcac gcttgcggat gtcgttggtg gccttttcgg
     9061 cggcttcctg cgtgatctcg gcgagcagct tctgctcggc cggcgacagc ctggccatgg
     9121 tgctgggtga aatcaccgtg agcagcgcgt cggagatgtg gccggtcagg atgatgttct
     9181 tctgcacttc atagaacttc ttggcttcga tggtgggcag cggattttcc tgcgcatcca
     9241 cggtgccgtt ctgcagcgcc aggtagacct cggcaaacgc gatgggtgtc gggttcgcgc
     9301 cgcaggcgcg cggcagcgcg gtgtaggccg gagaatcggg cacccgcatc ttcaggccct
     9361 tcatctggtc gcagttggtg aacagcttgt tgctggtggc gtggcgggcg ccatagtagg
     9421 tcagcgcggt caccgtgttg ccggtggcct tcttgtagcc ctcggccagt tccctgaaaa
     9481 catcgctctt gccgtacttg accacatggt ccgcgtcgcg gaaggtgaag gggtagtagc
     9541 tgacagccag gcgcggatag gagttggaag caaaggaggc gccggtcagg atgatgtcca
     9601 ccgtgcccag ttgcaggccc tggttgatgt cgctttcctt gcccaggctg gaagccggga
     9661 agacctggat ctcgtacttg ccgttggtgc gtttcttgaa ctcgtcggcc gcccagaccg
     9721 accacttgtg gtagggctcg gacgtttcat agacgtgcgc ccatttcagt ttggtctgcg
     9781 catgggccat gccaaatgtg cccagggtgc ccgccatgat tgcgcagcat gctattgttt
     9841 ttagagtgaa tcgttttgtc atgttgtctc ctcggttaaa agaaagggtc gaaaaggtca
     9901 gttgccgggc gcttccttca gcttgtcttc gcgcggcgcc agctcgcgct gaatcgggaa
     9961 tgggacttgt ccatgtgggc gtgcatggcc gtgcgggcgg cggggccgtc gtgtgcgcag
    10021 atggcggcca ggatcgcttc gtgttcggca atcgccgatt gccatgattt catcgtctcg
    10081 aagtagccgg tcaggcgcgt gaacagcggg ccgcggcggg agtcccagaa actctgcacg
    10141 gtttcgatca gcaccacgtt gccgcaagcc tcgacaatcg cgctgtggaa ggcccggtcg
    10201 ccgtccagcg gcaacacgcc gcggttggcg tcggtgacca tcgtgtcaat ggccgcgcgc
    10261 atcgcgtcga tgtccttgcg cctggcctgg agagcggcca ttgaggcgat ctcgccctcc
    10321 accacgcggc gcgcgcgtat cagttccagg ggcccccatt cggtcagggt gacctgcggc
    10381 cccttggaac ggctggcccg caccgaccgg tccagcacat acacgccgga gccggtgcgc
    10441 acctcgaccc agccttcgac ttccatggca atcagtgctt cccgcaccga cggccggctc
    10501 acaccgagct gtttggccag gtcgcgttct gccggcaggc gtgtgccggc cgcaaactct
    10561 cccttgctga tcagcgagcg caactgctcg gcgatctggc ggtaaaggcg ttgcggttcg
    10621 acggtttgca gcggcatttt tcggtactgg gagggattaa gggttagcca gaagtggaca
    10681 agtggtcaga ccaattgata atcccatgat tccagtcctt ccggccatcc ggctaaaccc
    10741 tgtaatgttt cctgtcatgt ttttcgtccg cgtccgtcca tgaccgcgcc cgtcacgatt
    10801 gagtccgtcc gcctgcggca ggtcaacctg ccgcccaagg tcgtgcgcac cgacgccatc
    10861 cagtcctttg tgacgcagga gaccctgctg ctcaccctgc gctgcagcga cggcgtggaa
    10921 ggcaccgggt atgcctacac cattggtacc ggcggatcat cggtcatggc cttgctgcac
    10981 gaccacctgg cgccgcgtct gctgggccgc aacccgctgc atgtggaggc gatctggaaa
    11041 gacctgtttt ttcacaccca cgccacggcc gtgggggcca tcaccagcct ggcgctggcc
    11101 tgcgtggaca ccgccttgtg ggactggcgc gcacgccgcc aggacttgcc cctgtggcaa
    11161 ctgctcggtg gcgcgcaatc acgtgtcccg gtgtacacca ccgagggcgg ctggctgcac
    11221 ctgggtgccg acgaactggt gacgcagacg ctggcggccc aggcccaggg tttccggggt
    11281 gcgaagatca aggtgggacg gccgcatgtc agcgaggacg tggcgcggct gtccgccgtg
    11341 cgggatgcgg tagggccggg cttcgacatc atggtcgacg cgaaccaggg cttcaaccgc
    11401 ccggaagcgg tgcgccgggc ggctgccttt gcggggctgg acctggcctg gctggaggag
    11461 cccatggcgg ccgaagacgt gtcggggcac cgtcagctgc gcgcccagac ggccatcccg
    11521 gtggccgtgg gtgagtcgat gtaccacctg agccagttcc gcgaatacct ggagcagggc
    11581 gcctgcagca ttgtccagcc cgacgtggcg cggattggcg gcatcacgcc gtggatcaag
    11641 gtggcgcatc tggccgaagg ttttggcgtg cccgtgtgtc cgcatttcct gatggaactg
    11701 catgtgagcc tgtgcgctgc ggtgcccaat gcggcctggg ttgaatacat tccgcagctg
    11761 gacgacatca cgaccagccg catccgggtc gagggcggtt atgcctggcc gcccgacacg
    11821 ccgggcctgg gcatcgcgtg ggactggcct gtcatcgaaa aacggcagct tgccgatacc
    11881 accctgaatc aataaaccaa ccaaggaaaa cacatgagac ttcaagggaa aaccgcactg
    11941 atcaccgccg ccggccaggg cataggcaag gccagcgcgc tggccatggc ggcagaaggc
    12001 gcgcaggtct gggcgaccga cgtcaatgcg aagctgctgg acagttatgc gggcatgccc
    12061 aatatccaca ccgtggcgct cgacgtgctc gacaaggcgg cgatccgcaa agtcatcgct
    12121 gacatgcccg cgctcgatgt gctgttcaac tgcgcgggtt ttgtgcacgc cggcacggcc
    12181 ctgcaggcca ccgacgaaga gtgggagttc gccttcaacc tcaatgtgcg ttcgcagttc
    12241 tggaccatcc aggcggcgtt gccgcgcatg ctcgcgaaca acggcggcaa gggcagcggc
    12301 agcatcatca acatggccag cgtcgtcagc atcaaggggc tgcccaaccg ctttgtgtat
    12361 ggcgcatcca aggcggcggt gatcggcctg accaaaagcg tggcggccga ctatgtgatg
    12421 cagggcatac gctgcaactg catctgcccc ggtaccgtcg atacgccttc gctgcaggac
    12481 cgcatcaatg tgtttgagga cccggcggaa gcacgcagga atttcattgc ccgccagccc
    12541 atgggccggc tcgcgcaagc ccacgagatt gcaccggtcg tgaccttcct ggccagcgac
    12601 gaagcggcgt ttgtcaccgg ccagcactac aacatcgatg gcggcatgac catatgaatg
    12661 agctcgactt ccagggccgg cacgcggtgg tcaccggtgg cgccaccggc ctgggctacg
    12721 ccattgcgca gcggctgatc gagtccggcg gcaacgtcac gctgtgggac cgcgacgaag
    12781 cggcggcaag gcaggcggcc gccgcgctgg gtccgcgggc ctcggcggtg gcggtggacg
    12841 tgtcgcagca tgcctcggtg cgcgccgcgg tccaggcgac gttgttgttg gctaccaagg
    12901 tcgatgcgct ggtcaacagc gccggcgtga ccggccccaa cgtcaagctc tgggactacc
    12961 cggtcgacgc gtgggagcag gtgatggccg tcaacctcaa cggcctgttc ctgtgctgcc
    13021 gtgaatgggc accgcacctg cgcgccaaca actacggccg catcgtcaac atcgcctcgg
    13081 tagccggcaa ggacggcaac ccgaatgcca gcgcctacag tgccagcaag gcgggggtga
    13141 ttgcgctgac caagtcgctg ggcaaggaac tcgccgatac cggcgtgcgc gtcaattgcg
    13201 tcacgccggc tgccgtgaag accgccattt ttgaccagat gacgccggag cacattgcct
    13261 tcatgctgtc gaaaataccg atgggccgct tcggcacgcc cgacgaggtg gcggccatgg
    13321 tggtctggct gtgcaccgaa gagtgttcgt tttcgaccgg cgcggtgttc gatctttcgg
    13381 gcggccgctc cagttattga gcgccccatt tccctctctt tttaaaaagg aaaaaccatg
    13441 aaactcgttc gttacggcaa ccctggcaag gaaaaaccag gtctcattga tgccgagggc
    13501 aaactgcgcg acctgagcgg cgtgatcacc gacatcggcc cggaccagct cggtgatgcc
    13561 gcgctggcca aactgcgcaa gctcaagacg gcgaacctgc cgctcgtcaa gggtaagccg
    13621 cgtctgggca gcccggtcgc cggtgtcggc aagttcatcg ccatcggcct gaactatgcc
    13681 gaccacgcgg ccgagtcggg cctgccgatt ccgaaagagc ccatcgtgtt catgaaggcc
    13741 accacctgca tccagggggc caacgacccg gtcatgctgc ccaagggttc ggtcaagtcc
    13801 gactgggagg tcgaactcgg cgtggtcatc ggcacccgcg cgcgttatgt ctcgcagaag
    13861 gatgcgctca attttgtggc cggctactgc accatcaacg acgtcagcga gcgcgagtac
    13921 cagatcgagc gcggcggcac ctgggacaag ggcaagggct gcgacacctt cggtccgatc
    13981 ggcccctggc tggtcacgcg cgacgatgtg ccgaacccgc aaaagctggc gatgtggctg
    14041 gacctcaatg gccagcgggc gcagaccggc agcacgaaaa ccatgatttt cggcgtggcc
    14101 aaaatcatca gttatgtcag ccagttcatg acgctgatgc cgggcgacgt gatcaccacc
    14161 ggcacaccgc ccggcgtggg catgggcatg aagccgcccc tgttcctgaa aaagggcgac
    14221 gtcatgaccc tgggcattga aggcctgggc gagcagcgcc agatggtggt tcccttcaag
    14281 ctttgaacca gccgagacgc tgctgtaaca gggtaaaaac aagggtttac ccttgggtga
    14341 cctagaaaaa cttctctaga tatcccggtc tctggcacgg gggcatggaa atgctgcaaa
    14401 atagaacggt cgttcttttt tgtctggctt tccatgtccg ttaccgaact tcctgtcaag
    14461 acagcccgcc cgggtccccg ggaaagtggt cgtgcagcgc atgccggcat gcaaaaaggc
    14521 cagcagacca aggcggccat cgtggatgcc gccctcggac tggccacgca gatcgggctg
    14581 gagggcctga gcattggcgc tctggccgaa gtcatgcgca tgagcaagtc gggcgtgttt
    14641 gcccactttg gctcgcggga agaattgcag atctcggtca tccgcgagta tttcgcgcgt
    14701 tttgaagaag aggtttttta ccccgcgctg aaagagccca aaggcctgcc gcgtgtgcag
    14761 gccctgttcg gcaactggat gaaacgcgtc gcggtcgagc tgcagtcggg ctgcatcttc
    14821 atcagcggtg ctgtcgaatt tgacgaccgg cccggcccgg tgcgcgacgc gctcgccacc
    14881 tcggtgcaga cctggctggc ggccatgtac cgtgcggtgg tgttggccaa ggaggccggc
    14941 cacctgcgcg cggacgccga cgagcgccag atcgccttcg agatccacgg cctgatcctg
    15001 gccctgcatt acgaagcccg cttcctcaag acgcccggtt ccatcgaccg gacattgcgc
    15061 gccttccaga gcatcctggc gcgttacggc agcgacgccg ccggcgcgct ggcgccaccc
    15121 atcacctctg catcactgac gggcggcagc gcccggccgg tgcctgattc ttccgcttcc
    15181 tccgcttctt ccgtttaatc ccttcccatc taaggacctt tccatgcctc aatacaaccc
    15241 gccactgcgc gacatgcagt tcgtcatgca cgaagtgctc aaggtcaccg acgagttcaa
    15301 ggccctgccg aaacacgccg atgtggac
//
LOCUS       DAIDFG010000037        15272 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_37_length_15272_cov_17.874351, whole genome shotgun sequence.
ACCESSION   DAIDFG010000037 DAIDFG010000000
VERSION     DAIDFG010000037.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 15272)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15272)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15272
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37_length_15272_cov_17.874351"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2744 a         5030 c         4856 g         2642 t
ORIGIN      
        1 aagcctgatc ggcaagctgg cgcaccggct gatgctgctg cccggcgtgc tgggtgtgcg
       61 cgtgaaaatt gccaaacttg aaatttttga cgactgcgaa gtggccattc gcgtcgagac
      121 cggccagtgg tgaagcacca caccgacaac ctgaggtaaa tccatgagtg ccgtattgaa
      181 tgaaatgacg aatgacgcag ccggtgaaga agcggcggcc acggccaaag cgggcctgaa
      241 gatagaacgc gaggaacaca agctcgaaaa acgcctgtgc cgcgaggtcg gccgcgcgat
      301 tgtggacttc aacatgatcg aggaaggcga caaggtcatg gtttgcgtct cgggcggcaa
      361 ggacagctac gcgatgctgg acatcctgct caagctgcaa aaacgcgcac ccatcaactt
      421 cgagctgatc gccgtcaacc tcgaccagaa gcaacccggc tttcccgacc acatcctgcc
      481 gcaatacctg gaaaaactcg gcgtgccttt ccacatcgag acgcaggaca cctacagcat
      541 cgtcaagcgc gtgatccccg agggcaagac catgtgttcg ctgtgttcgc gcctgcgccg
      601 cggcattctg taccgggtcg ccgaagagct gggcgccacc aagatcgcgc tgggccacca
      661 ccgcgacgac atgctgcaga ccttctttct gaacatgttt tttgcctccc gcctcaaggg
      721 catgccgccc aagctggtca gcgacaccgg caaacacgtg gtgatccggc ccatggccta
      781 tgtgcctgag aaggacctca tcaagtgggc gcaggtgcgc gactttccga tcattccgtg
      841 cacgctgtgc ggcagccagc agaacctgca gcgcgtgcag gtcggcaaca tgctgcgcga
      901 atgggacaag aagtttccgg gccgcctgga gaacatgttc acctcgctgc aaaacgtcgt
      961 gccctcgcac ctgatggacg gcaagcagca cgacttcaag ggcatacgca gcaccggcat
     1021 gccggacccg gaaggtgaca aggcctttga catggaagag ttttttgaac ccgtccggcc
     1081 cgggctgggc gtcgtgacgc tgtaagtttt acaagtcatt cagacctgga ggccttatgt
     1141 acagagcgct gaaagctatc atttttgcag caaccgtggc gggactcctg ggcggttgcg
     1201 ccagcacccg catggtcggc agcgaggtca acagcttcgc ccgctgggcg ccggcaccgc
     1261 ccgcaccggg cagcagctac cgcttcgagc gtttgccctc gcagcaggcg gacatatccc
     1321 aagcccggct ggaaggactg acccaggccg cgctggccag agtgggcctg cagcacaacc
     1381 cgcaggcggc cagcctgagc gtgcaggtca gcgccagctc ccagcccatg ctgcgctcgc
     1441 gctgggacgg cggctattac ccttatggcg gtggtcccaa catcttcatc ggcggcggca
     1501 gcggcgtggg tgtcagcaat gttggtgtgg gtttcggctt cggttttccg ctgggcggca
     1561 tggacacgcc gatttaccgg cgcgaagtca gcatcgtgat gcgcgagctg cgcagcaacg
     1621 tggccgtgta tgaaacacgc gcggtcagcg aggaaccctg gctggacacc gacccggtgc
     1681 tgtcggccat gctggacgcc gccctgcgcg gcttcccggt gccgccggcc ggcccgcgcc
     1741 gaatcaatgt cgaggtcaag ccttgaccca cggggcacgc gtgctggcca tccgccacgg
     1801 cgaaaccacc tggaatgtgg atacccgcat ccagggccag ctcgatgtcg ggctcaacga
     1861 caccgggcgc tggcaggccg agcgcctggc ccgcgcgctg gccgaagaac ccatacgcgc
     1921 gatttacgcc agcgacctcg ggcgcgccca tgacacggcg atggcgatca gccgcgtctc
     1981 cggtgtggcg gtcaccgccg aacaaggcct gcgcgagcgg catttcggcg aattccaggg
     2041 caagaccttt gccgagatcg aagccgttct gcccgagcag gccaggctct ggcgcacccg
     2101 cgacccggct tttgccccga ccggcggcga gtcgctgctg cagttgcgcg aacgggtggt
     2161 tgccaccgcc gaaaaactcg cagcgcgcca tgccggcgag ctgatcgtgc tggtcggcca
     2221 cggcggcgtg atggacgtgt tgtaccgggc cgcaacgcgg ctggacatcc aggcgccgcg
     2281 cacctgggca ctgggcaatg cggccatcaa ccgcctgctc tggacgccgg acggtttcac
     2341 gctggttggc tgggccgaca cccagcacct ggacaacgac aacaactccc tcgatgaaac
     2401 gaccacctga tgcacctgct gtctgacctg ctgggccgcc ctgccgcggc catcgacacc
     2461 cccgcgctgg tgatcgacct cgacgcgatg gagcgcaacc tggaccgcat ggcggcgttc
     2521 gcggcgcagc acggcgtcat gctgcggcca cacgccaaga tgcacaaaag cgccaccctg
     2581 gccaaactgc agatggccca tggcgcggtc ggtgtctgcg tccagaaaac ctcggaaacc
     2641 gaagcgctgg cggcgctggg cgtcaacaac atctacatca gcaacgaggt ggtctcgccc
     2701 ttcaagctca agcgcgtcat ggcggttgcc agggagctgc aggcgctggg cggccagctg
     2761 gccatcgcgg tcgacagcct cgagggcctg cgccggctgg cccaggctgc gcacggccag
     2821 catgcggcca tccgcgtgtt catcgaaatc gatgtcggcc atgggcgctg cggcctggcg
     2881 cccggcgcgt ccgccgtcgc actggccaat gaaatggcgc tccacccggc gctgcaattt
     2941 ggcggcctgc aggcctacca cggacgcgcc cagcatctgc gcaccgtggc cgagcggcaa
     3001 aaggcgataa cgcaggtgat cacccatgtg caggccaccc gcgccgcgct gcaggcggcc
     3061 ggccacgcag tgccgctggt gaccggcgca ggcaccggca cctttgcgct ggaaaccgcc
     3121 agcggcgtgt acggcgaatt gcaggcgggc tcctacctgt tcatggaccg cgactacgcc
     3181 gacaacgaac gcgacccggc gcagccggtt tttgagcacg cactgttcgt caaaagccag
     3241 gtgatgagcg tctgcgccag ccatgccgtg gtggacgccg ggcacaaaag ccatgcgatt
     3301 gattcgggcc tgccgacggt gcacggcctg gcgctggact acgccaacgg cggcgacgaa
     3361 cacggcatcc tgcgcggcgc ggtgctgccg gcgctgggcg acaccgtgtg gctgatcccc
     3421 ggccactgcg acccgaccgt caacctgcac gagcacctgc tgggcgtgcg cggcggcctg
     3481 gaacatggca cggtcgagca gatcatcaag gtggatgcca gaggcgccct gacctgaact
     3541 gacgcaggcg tcagatggcg atactcgccc cggtgcaagc gagccggtcc agcgtttcct
     3601 cccagcggct caggtgttca ccgtgcagag cagtgccttc ctccagcacg ggccagaatg
     3661 cccaaccgcg aagaccactc accacaaacc actggcgcac actttgccgc acgacccact
     3721 gcggcgtgcg ccagggtccc cagggctcgg cacttcttgc aaagtcaggt gtctgcgcca
     3781 aagcactcgc cgcataggac agcagtccgt aacgacgcgg ctggctgggc tggcttgggc
     3841 cgtcgtcata agtggcgatt cgcgtccggt cggccagcgc cgcaggcagc agttcgctgg
     3901 tcgtgaggtg aaagcctgtt tcatggggcg tgcctttgac gggatgcaga accggcgcca
     3961 gggcccagcc ggtcaacccg ctgcatcgca aggcctgggc cgcatcgatt ccgcaggcgt
     4021 attcaccggt ccattggtca acgagcgcga tcgtcgacgg cggcaccggc ttgctcagat
     4081 aaattttcgg tggcaggcgc acgcgccact cgcttgcggt cgggtgcagg ctgtccgcgc
     4141 tccaggccgc gcgggtcgcc gttacatcgg agcgcgattg ggcgatggtg ctgcggcaca
     4201 ccacttcaag atgcgatgcg gcatcgatct caccaccgga aaactgcatg tcacgcagca
     4261 gcgacagcga ttcgccatgg tcctgggcac atcgcagcac acgtgcgagc accggaccag
     4321 tcgcatcgaa gaacctggtg aacacatgtc ccactgccgc gttgggccgc gcaccaagcg
     4381 ccttggccaa cacggcgtcg gcggccagct cccgggacag cgcgggggag caaagggtca
     4441 ggcgcagcag gatcttcatt cttgtcgcga ggtcgccatg accccaaagt ggcaaatggc
     4501 gttttttacc acgcccgctg tatgccggcg ccggtctcag ggttggcgct aaggcggcgc
     4561 caatcaacgc aggcgacaat tccggaccat gaccccaagc cagatcatcg ttctcgccac
     4621 acccgtcttc tttctgctga tcgcgatcga gttcgcctgg ggctacgcga agggccgcaa
     4681 cacctaccgc ctcaacgacg cgatcaacag catcagcctg ggcatgctca gccaggtcag
     4741 cgcggtgttc acgcggctgt ttcgcatcgg catctacacc gccatctatt cgtcgatggc
     4801 gctgtttcct gacgaagcct tctggaccac ctggtacggc tggctgatcg ccctggtttt
     4861 ctacgacttc tgctactact ggctgcaccg cgccggccac gaaagcgccg tcttctgggc
     4921 cgcgcatgtg gtgcatcacc agagccagga ctacaacctg tccaccgcac ttcgccagac
     4981 cagcagcggc gcgctgctcg gctggatttt ttatgtgccg atggcgattg ccggtgtgcc
     5041 gccgctggtc ttcggcgtgg tggtgctgat cgacctgctt taccagttct gggtgcatac
     5101 cgaacacgtg cccaagctcg gctggttcga ccgctggttc tgctcgccct ccaaccaccg
     5161 cgtgcaccac gcagtcaacg acaactatgt cgacaggaac tacggcggca tcctgatcat
     5221 ctgggaccgg ctcttcggca gcttccgcga ggaagacgag aaatgtattt acggcacacg
     5281 cagcccgctc aacagctggg acccgctgtg gtccaacacc gaggtgtact gggccctggc
     5341 caaagactcc tggcatgcca ggaactgggg tgacaagctg cgtatctggt tcaagccgcc
     5401 tggttggcgc cctgctgacg tgacggcacg ttttccgaaa ccgccttttg acatcacgcg
     5461 gctggagcgt taccacccac ccatgacccg caccctgatg tggttcggcg ccgtgcagtt
     5521 tgcccttctg ttgcagggcg tcgccctgtt cctctggtac gccgacggcc tgccggcctt
     5581 gcagtcggcc gtctggctgg gggcgctggc gacagggctc tgggccgtgg gcggcgcgct
     5641 gcagggccgc ctgacgatga ccgaggtgct gctgatcgag gccgccgcgc tggcgacggc
     5701 gaccagcgtc cttgggctgg tggagctgca ccgcgtgttc aagccgctgg cgctttgttt
     5761 tgctcttgtt tttgtagctg ctcgcgcata ccccataagg gctgttggcc gatttgatct
     5821 gcttttgggg gcaggtctgg tcttttccct cgccggcgat gttctcctga tgtttcccgg
     5881 tttcttcatc cccggcctgc tgtctttcct ggtcgcccac ctgttctaca ttgccctgtt
     5941 caagcagggc caggcctggt ttcccagccg gcgcgcgctg gcggtgacgc tgggcgtggg
     6001 tgcgctgatg tacgccttcc tgtttgccgg gctcaacccg gtgctgcgtt ttgccgtggc
     6061 cgcctacgtg atcgtgattg cactgatggc cgcgcaggcg atcggccggg ccacggtgct
     6121 gcgtgacaag gcttcactgg gcgtggccgt cggtgccggc tttttcatgc tcagtgacgc
     6181 gctgctggcc accaacaagt tcgcccagcc cctgcccatg gcccagctgt gggtgctggg
     6241 cacctactac ctcgcgcaag tcctgatcgt gcacaacgcg cggcctgcca ctgcagggga
     6301 gcgggcgggc tgaacgcaaa gccgcaaggc cgctcagcac gcgacgtgca tctcgcggcg
     6361 atagggaagc gcatccgtcg gtgccggcct acagatggcg ggctcatggg ttcctatgct
     6421 tttgcattta aacaggagtt ttcatgcaag agccatccac cgaccgccac cccatcagca
     6481 acctggaata tgactggatc acgctgatcc agaacaaggc acaagcgctc atggcctacg
     6541 accaatacat caaggatgcc gagcaggccg gctccgccga atgcgccgca tttttccgaa
     6601 aggtgcacga cagcgacaag gcacagctgg ccgaggccaa gggacacttg gtcgccgtgc
     6661 ttcagggaaa aatgggcaag taggcgcacc acgcccggcg aacgccgcca ggcggagttc
     6721 gccgggacag tcagtctgct tggcaccagc gaccggacgc cgtgcacaca ctgaccggcg
     6781 taccgttcag ccagacctgc tctttgtcca gcagcaaggc ggtcgaaggg tcgatgtcga
     6841 agctgatccc cagggcctgc ccgacagcgg cattctcaat gggcagttcc caggtcagga
     6901 acaccaggct gcagtcctgc ccggctggaa aatcggccgc atcaaagcca gggccgcggc
     6961 gcttgatcac ggcatgcggc tgaccggtat cgaagaccac caccgtgccc cggcgcaggg
     7021 gaatgcgatg acctgttgcc gggaaatgca ggtccaggcc cttgtcctcg cctaggaaga
     7081 gattgcagaa ggccgcaccg ccatactgcg cgccatcgtg gtggtaacgc gcgccgcggc
     7141 aggccatcag ggcgacgtca caggcagcaa gctccacttg caggctcagc gtgtgtgtcc
     7201 agtccgacac cgcctgcacg cagcgccggt agtccggcca gcgcgcacgc gctcgcacca
     7261 aaggcaaggc ctcgacatct ccgggttcca gttccaggcg cagcgagatc tcccgttccc
     7321 agtcggccac cagcctggcc ggtggcgctg gcacgtcgac cgtggccgac agcacgatat
     7381 cactcacgct gcggctgcgc atgacatcgc cactgcggaa ataggaaacc agacggtctc
     7441 ccacgcgggg tgatggtgga aggggagcgc tcatttgccc cagtgtagtc aggggggcgt
     7501 gagccccttg gcgagggcgc ggccgcagag ctggcccaaa caccgtcgtt gcctgagaat
     7561 ggcacacctt gaactgaaat cgtgacggcg actttgtcaa attcaatata cctgtataaa
     7621 cacgcagtca atcaaactaa cgtcaatgac tacaaacatc ttttcaggtt cttccgcgcc
     7681 atattgtctg gcgaggtgcc atgttgttcc gcaccttttc gggacatacc aaagttaaac
     7741 ggcaagaatg ccccggcttc caatccgtac tcactttgtc gtagcgattg aactcgccgc
     7801 gttgattgcg tctgtcgttc tcgccgtatt gtggatgtca aagcccatag gagctttcga
     7861 gccctacctg gttgctacag gtctcgtctt tgtcgttact gaggcgtatc ggcgctatga
     7921 gggcaggctg ttccgaactg agggagtcga aagaacggca gctgaacgcg ttcgccacca
     7981 tgacgcgctt cgcgaccagt tcaaggagga gatcgaccgg tgcagggcag agaatctgcg
     8041 aaaagatgtg atcatccgcc acgtaaatcg gttggatgac tatccgaaca acaaagaagg
     8101 cccgggaatc tcaccgtggt tcaaggcagg tttgcttgac acctatcaca tgggcatcaa
     8161 agttggcctt gggtggtatg gcctcgttca aagtgaccgt ggattgcgac gtgcggacta
     8221 ccaaaatgcg gaggaaggcg agctgacagc aatgctactg ggcgagattc cttatgactt
     8281 tatagagtca atgaatgtcc gaggtgacga gtactactac cttccccaca tcttttgcca
     8341 ttttgctaat cacggcgaac cgtaccagcg gttgttctat gcacaggaaa ttagcatggg
     8401 ccacgggcat tcgtactgga aggagattgc aagttatgaa gaggtcaaac ggaatggcaa
     8461 ggcgagcgcc acgtgaagtt gctgtttaac atgtcaatcg acacggaccc acaacagcaa
     8521 gaggcggctg cgccgcgcgt gttggtggtt cggtcatttt cacggtaagc ggcaaatgga
     8581 gcggtcagcg gctgacgacg atctcgtaac ctgcccaagc tgcggccaac gcgactcgct
     8641 cgtcattcga tggatgcccg agatcgacta tcgcgtccac gctcggacag aggtcggctg
     8701 tgaacgttgt caattgtggc agtctgcgaa ggaggatcgg tgggccttcg ccgattggaa
     8761 ccactgggcc tgcgcagagt gggctcggaa aggtcaacag cacccgcatg caacgctgta
     8821 tgcactgctg gttaaagagg ggcagttgga acgcgcgcaa atggccgcgg ctgccaacgt
     8881 aagcgagtac cttaagaacg aagttgctag tcggtgcgta tggaagactg gcgaccgctt
     8941 cgagtcgctc gattggccaa gggggcgctg gtcagtgcgt tctgtcgagg ccgtctacgg
     9001 aacaaacacc gggcctttct cgatagtcaa agcaatcgag atactgccca gcggcattct
     9061 tggcgaggag aaacacgagt tttgggatca tcaagccaga ttaaggaggc tatcgccata
     9121 cgctcggccc agggaatggt ctcaagtcca cactggagac cgatgcctcc tcgatggatt
     9181 tcccggattg gtcctctccg cggacaccac caagaggttg gccgtcatca gaatcgacgc
     9241 cggcaatgag gaggttcaca ttgcacgcct cagcagcctt caagttcctg ttcaccgcct
     9301 ggagcgcgat gacgcctagc ccgccgtatg cggactcccg tacaagaggc atggccacgc
     9361 cgttgcggtc cgctcaattt cacgttaaac cccgtttttg ctagcgaccc ttctcgcatt
     9421 tttcttcaac aatttttccg agtccactct ctggtgaccg ttccttaagc acgctccgga
     9481 atagccacat ttccgctgtt ccccgctgat tctccgggat caaccggccg gcgcgtgggt
     9541 cacagcttcg tcccgcgcaa tgcgcccgtc gatcagctcg accagccggt cgcagcgggc
     9601 ggccaggcgc ggatcgtggg tgaccaccac aaaggtggtg ccgagttcga cgtgcatgcg
     9661 ccgcagctgc acaaacacct cgtcggacga ggcggtgtcg aggttgccgg tgggctcgtc
     9721 ggccagcacc agcgccggct ccatgaccag ggcgcgggca atcgccacgc gctgctgcat
     9781 gccgcccgag agttcggccg gccgcttgtc catggcgttg gccaggccca ccgaggccag
     9841 cagcgcgcgg gcatgctcgc gttgcttcgt gctgatgcgg ccgccgcgca tcagcgctgg
     9901 cagcgtgacg ttttccagcg ccgtgaaggc cggcagcagg tgatggaact ggaacacaaa
     9961 gcccagggtg ctgcgccgca gcagggtcag ggctgcgtcg tccaggccgt gcacctcctc
    10021 gccctgcagc cggtagctgc cagccgtcat gccctcgagc aggccgacga tgttgagcag
    10081 cgtgcttttg ccggagccgg aggggccgat cagcgcgatg aattcgccgc gttgcacctg
    10141 cagcgacacg ccgtgcaaca cctcggcctc gctgggcagg ccgaggttgt agctcttgcg
    10201 cacctcgccc atgtcgatca gcgcgcggga atcggtgggg gccgtcatgg ggtcacatcc
    10261 gtatcgcctg cgccgggtcc agcgccgcag cgcggcgcgc cggtgccacc gcggccagca
    10321 caccacacac ggtggcgatc agcgccacct gcagggccgt ggccaatggc aggccgatca
    10381 caaacagcgg cagcccgtcg gagccgcgca caaatttcgt gaacagccag atcatcagca
    10441 cggccagcgc aatgccgagt gccgagccga gcgcgcccac caccgcgccc tgcaccagga
    10501 aaagccgcag gatctgcccg cgcgtggcgc ccatggcgcg caaaattccg atctcgcggc
    10561 gcttttgcac caccgacacg accagcacgc tggcgatgcc cagcaccacc accaccagca
    10621 ccaccccgcg tatcagcgcg gtgctgaccg actgggcgtt gagggctgac accagctgcg
    10681 cgttgctctc ctgccagctt tcaatcttgt agggaaactg ccggcgcagg tcactggcaa
    10741 gctcctgcgc cgaccacacg tccttcacgg tcaggtccag actggtggcg ccgccgggca
    10801 gacccagcag gttttgcgcc gcgcgcagcg gcacgatgac ggtgcgccgg ttcaggtcct
    10861 tgacgcccag gtccaccagc gcggtgacac gcaccgagtc gctgaccagg ccggtctgca
    10921 cggtcaggcg gtcaccggcg cgcacgccca gatcggccgc cagctcacgg ccgacgatgg
    10981 cctcgcccgg cgccagccgc gcggtgccgc tgatcacctt gctgcgcagc ccgacgacgc
    11041 ggtcgtagcg gtccagatcc acgcccagca gcgctatcgc ctgggtggct tcaccgcgca
    11101 gggccaggcc ggcacccgac accatggggg atacgccggc aatgccgggc agggcttcga
    11161 gcagcggcat cagggcctgc cagttggcga ccgaacgcag gcgctgcgcg cgcggctggg
    11221 tgtcggtcag cgcggtggtg cccggcacgg ccggccaagc gggcgtgacc acgtcgtccg
    11281 gcgcacgcac gctgatgtgc gcctgcgcgc ccagggtctt ggtcagggtg ctgccctgca
    11341 ggccgctgat cagcgccgaa atgtaggcga tgaccgccac accggccgcc acaccgacga
    11401 tgatgagcac cgtctgcatg cgcccctcgc gcaggaaacg cagcgccacg cgccattcga
    11461 aaccaagccc gggaaacatt cagcgcccga tagcgttgga catggccgag ccggcttcgg
    11521 acgtggccgc agccctggcg accggctgcc ccggcgtcca ggccacggcg cgcgcgcgaa
    11581 tgcgggcgcc ggccggcacg ctgcttccca gcagcacggc gtctccttca gccaggccct
    11641 cgagcacctc ggccgcgtcc agtgtgcgca ggcccaggcg cagcttgcgc tcctgcgcgc
    11701 gacccgcgtc ggcaaccagc accgtcgccg tggtgggcga ggcctggctg cgcagggcgc
    11761 tcagcggcag caccagggcg cgttcacgcc ggccggtttc gacctcgacc gagagcgtca
    11821 tgtcttcccg cagaaagacc ggggcctgct gctcgagcgc aaacttgact tcgatggcgc
    11881 cgcgctgggc atcgaccgcg ggggcaatcg acaacacgcg ggcggcaaaa cgctggtcgg
    11941 caaacgcgtc ggccaccacg gccgccttct gccccggctg cagctggtcg aggaaacgct
    12001 cgtccacctg cgccgtgagt tgcgtggggc cggccagcgc caggctcatc agggccttgc
    12061 cgggctgcac gatttgcccc ggctcgacat cgcgtgtcaa cacgcgcgca tcggtcggtg
    12121 ccagcacggc cgcctgcgcc aggcgggcgc gggccgctgc cgtggcggcg ctggccaggg
    12181 ccagctgcgc ctgggcctgg gccacatcgg tcccggcatc ggcgttggcc tgggtctgtg
    12241 cgcgggcgcc ggcctgctgc gcactggcca cctcgacggc gcgccggact tcatcgagcc
    12301 gcgcttcgct gagaaaaccc tgggccacca gttgttgggt acgtgcgagt tcggcctcgg
    12361 ccgccttcag cgtcgaatcg gcctgcgcga ggccggcctg ggcggccgtc cgcccggtgc
    12421 tgcgcaggcc tgccatgcgc gcctgggcct ggcgctccga agccagcgcc tgcgtcacgg
    12481 cggcgcgcag ctcctcggtc tccagctgga ccagcacatc gccctgtcgc acctgcgcgc
    12541 cttctgccac ccgcacctgc gcgacgcgcc cggtcacggt gctacccacg tccacgcgcg
    12601 acaaggtcgc cacgcgggca gaaaactgca gggtccgcag cagcggcgcg taacgcagtt
    12661 ccagcacatc aacccgggga cttttctgga caaaccaggc cgcgccggcc gccaccacaa
    12721 ccatggccac gacccctatc cagatccagc cgcgtttcag ggaaagggtg tgggccaggc
    12781 tcatggggag ttcaggttcc gggttcaaac aggccggcac cggttccggg cggtgtcacg
    12841 cccagatgcc ggtaggccgc cagtgtcgcg atacggccgc gcggcgtgcg ctgcagataa
    12901 ccctgctgga tcagataggg ctcgatcacg tcttcaatcg tgtcgcgctc ctcgccgatg
    12961 ctggcggcga tgttgtccag gccgaccggg ccgccgtcga atctgtgaat gacagcctca
    13021 agcagtttcc tgtccatcac gtcgaatccc tgggggtcca catcgagcat ggccagcgcc
    13081 ttgttggcga tgtccagggt gatgtggcct gcgcccttga cctcggcata gtcgcgcacg
    13141 cggcgcagca ggcggttggc aatacgcggc gtgccgcgcg aacggcgtgc aatttcaaaa
    13201 ccaccgtctt cgtccatggg cacgttgagc aggccagcac tgcgccgcac gatgcgcgcc
    13261 agctcgtcag cgctgtagaa ctccagccgc gcgacaatgc cgaaacggtc gcgcagcggg
    13321 ttggtcaaca tgccggcgcg ggtggtggcg ccgaccaagg tgaaaggctg caggtcgagc
    13381 ttgatgctgc gcgccgccgg gccctcgccg atcatgatgt cgatctggta gtcctccagc
    13441 gcggggtata ggatttcctc aacgaccggg ctcaggcggt ggatctcgtc gatgaagagc
    13501 acatcgtttt tttcgaggtt ggtcagcagc gccgccaggt ctttgggttt ttccagcacc
    13561 ggccccgagg tctggcgcag gttcacgccg agttcgtgcg caatgatgtg ggagagcgtg
    13621 gttttgccca ggccgggcgg gccgaacagc agcacatggt ccatggcctc gctgcgtttg
    13681 cgggcagcac cgataaaaat ctcgagctgc tcgcgtacct tggcctgacc aacgtactcg
    13741 tcgaacagct tgggtcgcaa ggcgcgttcg atcgcttcct cgttgggcga ggccggcgca
    13801 gcagacatga cccgcttcac aggcggaacg ccggacatgt cgaagtcgtc ggtctggatg
    13861 ctcatttagc gagggctttc aaagccagct tgatgccgtc actcacaccg atgtcttttg
    13921 gcagcgcctt gagcgccagg gctgcatcac ggtcgctgta gcccagcgcc accagggcct
    13981 gcaggatgtc ggattgggcg tcgtcggcca cgctgaccgt gatgcccatg tcggcgccaa
    14041 gtttgccctt gagttcgagc agcaggcgtt cggcggtttt tttgccgatg ccgggcacct
    14101 tgatgatgcg gccggcttcc tgctgggtca cggcctgggc cagctcgtcc acactcatgc
    14161 cgctgagcac gctgagcgcg gtgcgcgggc cgaccccgga aatcttgatc agctggcgaa
    14221 aggcggcacg ttcgctggcg ctgccgaagc cgtaaaggat ttgcgcgtcc tcgcgcacca
    14281 cgaagtgcgt cagcagcgag actttttcgc ccagaccggg gaggttgtag aaagtgctca
    14341 tgggcacctg cacctcgtaa cccacgccgt tgcagtccac cagcagttcc ggggggttct
    14401 tttcagccag caggccactt attcgtccga tcatgggcaa atcttagcag tcgtgcccac
    14461 gtgcccgctg acttgaatat cgggggacaa tcacccatct gtctgtgtcc cgccggttct
    14521 gaccggtcca accgcccgga aagcctgcat ttgattcttc gccacacttt caccccgccg
    14581 aacaacaccc gcatgggcca cctgtgcggc cccatggact cgcacatccg caccgtcgag
    14641 gcggcgctgc aggtcacgat tgcgcaccgc ttcgagcagt tcaagatcga cggacccaag
    14701 gccaaagcgc agcaggcact ggaagtgctg caggcgctgt atgaaatggc gcaacgcccg
    14761 attcccgagg aaaccgtgca gctgatgctg gccggcgaca ccgcgctgag catcgccccc
    14821 gacggcgcga tggcgctgtc cacccgccgc gccgacctgc gtgcgcgcac caccaaccag
    14881 ggcgcctacc tggaaaacat cgccacccac gacatcacct tcggcatcgg cccggccggc
    14941 accggcaaaa cctacctggc cgtggcttgc gcggtcgatg cgctggagcg cagcgcggtg
    15001 cagcgcatcg tgctgacgcg cccggcggtc gaggccggtg agcgtctggg ctttcttccg
    15061 ggcgacctga accagaaggt ggacccctac ctgcgcccgc tgtatgacgc gttgtacgaa
    15121 ctgatgggtt ttgaacgtgt gcagaaagcc ttcgagcgcc agcagatcga aatcgcgccg
    15181 ctggccttca tgcgcgggcg cacgctgaac cacgcctttg tgatcctcga cgaggcgcag
    15241 aacaccaccc ccgagcagat gaagatgttt tt
//
LOCUS       DAIDFG010000038        15122 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_38_length_15122_cov_19.456096, whole genome shotgun sequence.
ACCESSION   DAIDFG010000038 DAIDFG010000000
VERSION     DAIDFG010000038.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 15122)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15122)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15122
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38_length_15122_cov_19.456096"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2545 a         4890 c         5148 g         2539 t
ORIGIN      
        1 tgtttttctg gctgttggcc ctggtgttgc ggttgagcag gtgcttgacg cggtaggcca
       61 ggcgccccag ccaggtgcca tagatcacgt cctcgacttc cttgcggttg acgatcagaa
      121 cgcgcagcag ctcggtcagg tgcggggtgg tccagtcgcc agcgatgaaa ctttcggcaa
      181 agccgatgtc gcccgaacgc agcgcggcgc tgcaggcctt ccagttcacc aggcgcaggc
      241 tggccgtggg ggcctcgccc gagccgaagc gctgcatgct gccgtcgggc agctgcaggg
      301 tcagcgtgcc gtgtttgagg cgcgacagca gcttgaaggc cgtgcgcgcc gcggccggag
      361 cgccaccggg cagcgcaaac gaagcgccat tgactgaaga agaagcggtg gagttgctgg
      421 ccatggtcat cgggttacaa aaatttcagg gggctggggt ttgctgacaa caggcacacg
      481 tttgcgccag agcttgaggg cctgccaatg aatgcgggcc accacaccga aggtcatggc
      541 cgggtagccc cacagggctt tgcgcagcgt ggccgcgctg aggacctgca gggtgccggc
      601 gacgctggtt tgcagcagcg gccccgacgc gtcgtcgtag tcgatgcgcg ccactgtttt
      661 ctccacgcct tcattctcga cacgcatgaa acggaagcga tagaaaccgt ccagcgtgca
      721 gaacggcgac acgtgaaaca ccttgacggc ctgcagctcc tggccgtaac gcgggtggtc
      781 gagcagatag cagtggcgtt cgccaaaggt gttgttgact tcgacgacga tggcgcgtaa
      841 ctcgccggcc gcattgtggc aataccagaa gctgaccggc ttgaaggtgt agccgaacac
      901 ccgcgggtag gtgtgcagcc agacctcgcc cgcggcgtcg gtgatgccct cgcgctccag
      961 cagcgcatcg acccaggcca ggcagtcgtc gccgccctcg ccatgatcgc ggtcaaaaaa
     1021 gctcagggcc gcgccgcggt tgcgcgccag cgcgccgttg ccatgctggc gcaggctgcg
     1081 cagcggcagc atcaggaaat aggtggggta gttgaaggcg ttgacagcag gcttcaagcg
     1141 ggtgtgccgc acctcgccaa aaccgatcaa ggcttgtgca gggcttgcgc tcatggcgcg
     1201 gctccggcgc agtcctgcag cagcagtcgg gcggtttgca ggcccgactt caaaccgtct
     1261 tcatgaaaac cataaccggt ccaggcgccg caaaaatagc tgtgctgcag gccctgcagt
     1321 tcgggcagct ctttctgcgc gcggatggcc gcagcgtcga agaccgggtg ggcatagtcg
     1381 aattcaccca tgatgtgctg atctgcgatc ggggccgcgg gattgagcga caccaccact
     1441 ggctgggcaa acggcagggg ctgcagcatg ttcagcaggt aatgcaggca cacctgggag
     1501 gactcacgcc gcgggtccag cgcacgttcg tagttccagg cagcccaggc cagccggcgg
     1561 gcgggcagca ccgaaatgtc ggtgtgcagc acggcgcggt tgggctggta gctgatggcg
     1621 cccagcaccg tgcgttcggc actgctcggg ctttgcagca atgccagtga ctggtcggag
     1681 tgggtggcca gcacgacctt gtcgaactgt tcgtcggcgc cgtcggtgag cacgcgcacg
     1741 ccgccatgag gcgagagcgc atcgcggacg atgccgcgca ccggcgtgtt caggcgtttg
     1801 tcggcgatgc cggcaataat tttttcaacg taatgccggg cgccgccggt caccgtccac
     1861 cactgcggcc ggtcggccac ctggatcagg ccgtggttgt ggcagaagcg gatcatggtg
     1921 gccaccggga attgcagcat ctggtcggtc gggcaggacc agatgcagcc cagcatgggc
     1981 aggaaatacc agtcgcgaaa ctcggctgaa tactggccct cgcgcaggaa atcgccgagc
     2041 ggctgcatca tggcggcttc cgcgccgctt tcggccagcg ccgtggccgt gcggttgaaa
     2101 cgcatgatgt cgcggagcat gcgccagaac ttccagttca gcaggttgcc gcgctgggca
     2161 aacaccgtgt tcaggctgga gccgctccac tccagggcgc cgcgcttgcc ggcgccgggg
     2221 atcttgaccg aaaacgacat gtccgacctg gcggtggcca cgccgaggtc ggcaaacagc
     2281 tggatcaggt gagggtaggt gcgttcgttg aagaccagaa aaccggtatc gacgccgtgg
     2341 gtcaccgggc cttgcggcgt gggcaaggtg atgtcgaccg tgtgggtgtg gccgccaaaa
     2401 tagctgccgg cctcgaacag ggtcagttcg gcctggcccc gcaaggcgtg ggcgaccgcc
     2461 agtccggcaa tgccggagcc gacgatggcg accttggggc gggctggaga actcatctca
     2521 cagcccggaa gacaaaaatg ttgcgaagcg ccccgatgcg aacaagggag ggagggagga
     2581 ggaggagaac cgccccggga ttgcagccgg accgcgagcg gccggaagtc ttcgcaacaa
     2641 aaaaacagtg actgtcggcg ctgcgcttgc atgccagtgc cgaccgaagt gtgagtccgc
     2701 gaccccgccc acagttcccc tgctgggcaa gctggtgggt gtaaaaattc gttaacgtcg
     2761 cggaagtcat caaaaaatcg aggtgtgtgg agaagaggtt ctgtctgaca agttctaaat
     2821 tggacttagt ggttaaatta ttaccgattg gtaattattt gcctaaaagc ttctcttcga
     2881 tgtccttcag gaaggcaggg tctttggggt cggtggcggt gttgaagctg gtgacgcgct
     2941 ggccatcgcg ggccacgaca tacttgtgaa agttccagcg cggacgctgg ccggtcttgt
     3001 cggccagttg ccggaacagc gggttggccg ttttgccggt cacgccggat ttgacgaaca
     3061 tcggaaactt cacgccgaag gtgttttcgc agaaatcggc gatctccttg ttgctgccgc
     3121 tttcctggga aaagtcgttg gacggaaagc ccagcaccac caggccctgg tcccggtagc
     3181 gcgcatacag cgcttcaagg cccgcatact gccgggtgaa accgcagaag ctggcggtgt
     3241 tgaccaccac cagcaccttg ccgctgtact ggcacaggga ctggggcttt tcgtcctgca
     3301 gccgcaagaa gctgtgttgc agcaaagggg cgcaggccgg cgcctcctgg gctgcaccag
     3361 cgccgggtgg ggccgcacgg gcaggcgtga gggccgccat ggccgccagc agcgcccaca
     3421 tcggccaagg gttgaaagac gggtgtttca tgatcttgct cgcttttgaa taatgggaat
     3481 aatggacggc ccttgttgcg cagtctgtgc gccacaaaac caaactaccg caaacctgac
     3541 ctgactgccg gacatacgca gtgtgggtcc gatcagattc aagcatgttg ccccccgaac
     3601 cctccctgcc cggtccggtc attgcccgcc aggccgacgc ctgcgccccc ggcttcacga
     3661 ttgccgcggt cgagcgtgat accggcctgt ccaaggacac cttgcgggtc tgggagcgcc
     3721 gctacctctt tcccgtgccc ggccgcgacg cccacggcga gcgcctgtat tcacgcgagc
     3781 agctcgagca gctgcggctg atcaaacgcc tgctggacac cgggcaccgg cccgggcagg
     3841 tggtgggttt gccgcatgcc gagctgcagg ccctgggccg gcaggccggc ggcaacaggc
     3901 agcgtccgca ggggcagcgc ggcaaaaccg ccaggaccgc cgtgacggcg gcggtcgacg
     3961 ttcccgcgcc cgagatccag gcctgttttg agctggtcaa gcgccacgac gcgctgggcc
     4021 tgcgccacac cctggccgcc gccgtgcagg agctgggcct ggcgtctttc gtcagcacgc
     4081 tggtggcgcc gctcaacacc ttgatcggcg acgcctggat ggatggccgg ctggaggttt
     4141 tcgaggaaca tttctacacc gagtcggtga ccgccgtgct gcgccacggc atcagcagcc
     4201 tgggcgtgac cggcgtcgcg tcgccccggg tgttgctgac cacgtttccg caggagtcgc
     4261 atgggctggg tctgctgatg gccgaaacct ttctggccct gcagggttgc aactgcctgt
     4321 cgctgggcgt gcagacgccg gtgcccgaca tcgcccgcgc ggtgtcggcg cacggcgcgc
     4381 agatcgtggt gctcagtttc agcgccagcc tcaaccccaa ggatgtgacg caggggctgg
     4441 gcgacctgcg cggcctgttg ccgccggcgg tcgagatctg gaccgggggc gcctgcccgg
     4501 tcctgcagcg ccgcaatgtg ccgggcgtgc gggtgctgga cagctttgcc gccatcgagc
     4561 cagaactcca gcgctggcgc caggcgcagc cctgatctgg cgtggctcat ttttggcggc
     4621 gttggccgct acccaaggcc ggcgcaaggg tgtagaaggg ttgaagaggt gtgcaaagcg
     4681 ccatcccctg agccccgcat gaaagagggc tgaaaagcct gcttgaaaaa gcttgtcaca
     4741 ttcagcccct aaaatcagag tgaatctagt gcggtgtgtg ccgcgtaagg agttcgtatg
     4801 ctttacccag aactattcaa acagctcgaa tccgtccgct gggacatgga caaggacatt
     4861 ccctgggcga gtttcgacgc gtccaagctg accgacgagc aggcgcagac catcaagatg
     4921 aatgccatca ccgagtgggc ggcgttgccg gccaccgaga tgttcctgcg cgacaacaag
     4981 gacgacagcg acttttccgc cttcatgtcg atctggtttt ttgaagagca gaagcactcg
     5041 ctggtgctga tggaatacct gcggcgcttt cgccccgagc tggccccgac cgaaaaagag
     5101 ctgcacgacg tgcgtttcga gttcgagccg gccccgccgc tggagacgct gatgctgcat
     5161 ttctgcggcg agatacggct caaccactgg taccggcgtg ccagcgagtg gcacaccgaa
     5221 cccgtgatca agcagatcta cacccagctc agccaggacg aggcgcgcca cggcggggcc
     5281 tacctgcgct acatgaaacg cgcgatcaat aattttggca acgacgccag ggccgccttc
     5341 accaaggtcg gcgtgttgat ggccagtgcc aggcgcacgg cgcaggccct gcacccgacc
     5401 aacctgcatg tgaacaaggc cctgtttccg cgcgacacca tccagtcgcg cctgcccaac
     5461 cccgagtggc tggagcactg gctggacaag cagatcgatt tcgatgccgc ctgggaaggc
     5521 aaggtggtcg agcgcatcct gcacaacatg agcctgctga tgaaccagag tttcaagacc
     5581 gtgcaggagc tcaatcgcta ccgcaaggaa ctgagcaagg aaatcgcggc atcggtcgcc
     5641 ccggcgccgg tggccactgg cgcctgacgg cgggcggcgg cctgctggtc gagagggtcg
     5701 cgacggcctt ccgttaaagt ggcgtgatgc aaggcgcatc ccctcccccc tcttctcccc
     5761 ttttcacgca aaaaatcgtt tcccgcgagc aggcggcaca gcttgtcgcg gccctgccgc
     5821 ggccggtcgt gttcaccaac ggcgtgttcg acgtgttgca tcgtggccat gcaacctacc
     5881 tggcccgtgc acgggcgctg ggcgccagct tggtggtggc gctcaacacc gatgcgtcgg
     5941 cccggcgcct gggcaagggc ccggaccggc cgctcaacaa cgaggaagac cgcgccgtgc
     6001 tgatcgcggc gctcgaatcg gtgagcctgg tgacctggtt tgacgaggac acgccgctgg
     6061 aactgattgc cgaactgcag cccgacatcc tggtcaaggg cggcgactac gacatggata
     6121 cgctcgccga gaccgcggtg gtccggcgtt acggcggcca ggcgctggcg attccgtttg
     6181 tcgacggcta ctcgaccagc gcgctggtgc gcaagatccg ccaggccccc tgatcctgcc
     6241 cttgatctcg aggcgctact gattcaatag ctggtgacgc ccgtggaata agggttacag
     6301 gccatttcga ctcgcattcg aacgggccgc ctccggctca gggcgccggc aactgcgccg
     6361 gcttcgcatc ggccgccagc gttgcctgcc cagcctcggg cgattccttg cggccgcgca
     6421 accggttggt caggcgccgg atgccatgtt cgatgtcgtc gatgtaagtg aacaccgccg
     6481 gcaccaccag caggctcagc accgttgagg tgatcagtcc gccgatcacg gcaatcgaca
     6541 tcggcgagcg aaaactcgga tcggccgcac cgaagccgaa ggcgatcggc atcatgccgg
     6601 cgcccatggc cagcgtggtc atgatgatgg gccgggcccg cttgtggcag gcgtccagca
     6661 gggcgtcgcg gcgcgacatc ggcgccacca ccgggtggcc gttgctgccg tcgttgccgc
     6721 ggcgcgcgac gatggcgtat tcgaccagca ggatggagtt tttggtggca atgcccatca
     6781 gcatgatcag gccgatcagt gagggcatgg aaaaactctt ctgcgcgatc agcagcccga
     6841 cgaatgcacc gccgagcgcg agcggcaacg cggccaggat ggtcaccggg tgcaggaagt
     6901 ccttgaacag cagcaccagc acgatgtaga tacagagcac gccggtcagc atggccaggc
     6961 cgaagctggc aaacagctcg cccatgactt cggcgtcgcc gatctccacc accttgacgc
     7021 cggcaggcag gtttttcacg gccggcaatt cctgaaccag cttggtcacg tcgcccaagg
     7081 gcaggcccga cagctcgatc tcgaagttga cattgcgcga gcggtcgtaa cggtcgatca
     7141 cggccggccc gccggcaatc tccagcgtcg cgacctggcc cagcatcacc gggcccttgg
     7201 tcccgggcac cgagaggcgc tccagcacgc tgaggtcctg gcgcgccgcg tcggccagct
     7261 tgaccacaat cggcacctgg cgttcgggca ggttcagttt gggcagcgac acgtcgtagt
     7321 cacccagggt cgcaatgcgc agggtctcgc cgatggccgc gctcgtcaca ccgaggtcgg
     7381 cggcgcgcgc gaagtcgggc cgcacggcga tctcgggccg gatcaggctg gcggtggacg
     7441 ccacactgcc caggccaggc acggtgcgca ggtctttttc cacagcccgc gccgcggcct
     7501 gcagggccac cgggtcttca ccggtcaaca ccagcacgta cttctctccc gagccgccca
     7561 ggccgacctt ggtgcgcaca ccgggcagcg cttccagggc agcacgcatt ttgttttcga
     7621 tgccctgctt gcgcggcctc tcggtgcgct cggtcagcag caccgtcaag gtggccttgc
     7681 gcgtttcggc cgcaccggcc atggcaaacg ggtcggtgcc cgcgctgccg ccgcctatgg
     7741 tggtgtagac gctgcgtaca tgctcgacat tgctgaccag ttgccgcgcc tgctcggccg
     7801 aggccttggt ttgcgccagg gtggaaccgg gcggcagctc gatgtagacc tgcgtctgcg
     7861 agttgtcgtc gggcggaatg aagccggtgg gcagcagcgg gatcagcagg atggagccga
     7921 tgaaaaaaac cgtggccgcg atcatggtgg tcacgcggtg cttcaggcac caggtggcca
     7981 tgcgcatgta gacgcgcatc caggccgggt ccttgtgctc accgacgatg ggcttgagca
     8041 ggtaggccgc catcatgggg gtgaggacac gcgccaccac cagcgaggcg aacaccgcca
     8101 gcgatgcggt ccagccgaac tgcttgaaaa acttgccggc cacgccgctc atgaaggcgg
     8161 tcggcaggaa caccgcaatc agtgtgaagg tggtggccac caccgccagg ccaatctcgt
     8221 cggctgcctc catcgctgcc tggtagggcg tcttgcccat gcgcaaatgc cgcacgatgt
     8281 tttccacctc gacaatcgca tcgtccacca ggatgccgat caccagcgag agcgccagca
     8341 gtgtcacgac attgatcgag aaacccagca ggtacatgcc gatgaaggcc ggaatcaccg
     8401 acagcggcag cgccacggcc gacacaaaag tggcgcgcca gtcgcgcagg aacagccaga
     8461 ccaccagcac cgccagcaac gcgccttcgt agagcagcgc catcgagccg tcgtactcct
     8521 cttccacagg cttgacgaag ttgaaagctt cggtcagcac caggtcggga tgttgcgcct
     8581 tgagttcagc caacgccacc tgcacggccg cgccgacggt gacctcgctc tcgccgcggc
     8641 tgcgtgccac ttcgaaaccg acaacgggct tgccgtcgag cagcgcggcc gaacgcgggt
     8701 cggcaacggt gtcgctgacc gtggccacct ggtcgagccg gatgcgccgg ccgtcgctga
     8761 gcgccagctc cagtcggccc agctcctgcg ccgacttgac ggtggccagc gtgcgcaccg
     8821 gctgctcacc gccgcccagg tcggtgcgcc cgcctgcgct ttcggtctgc acctgcttga
     8881 gctggcgcga gatgtcggcg gcactggcgc ccagcgcctg cagcttcgag ggatcgagcg
     8941 ccacgtgcac ctcgcgtgtc acgccgccca cgcggttgac cgcacccacg ccgcgcacgg
     9001 ccaacagctt tcgcgagacc gtgttgtcga caaaccagga cagtgcctcg gcatccatgc
     9061 ggggcgaact gatggtgaag gccagcaccg gctggccggc caggtccacc ttgctgacaa
     9121 tcgggtcgcg gacatcgccg ggcaggtcgg agcgcacccg cgcgaccgcc gaacgcacgt
     9181 catcgacggc ttcctgcacc ggtttttcga ggcggaactc ggcagtgatg gtgacgccgc
     9241 cgtcctgcac cttggtgtag atgtgcttga ggccctgcac cgtggcaatc gagttctcga
     9301 gcttgcgcgc cacctcggtc tcgagctgcg ccggcgcggc acccggcagt gaggccgaga
     9361 tgctgacggt cggcaggtca atgtcgggaa agttctgcac cttcattgcc ttgaacgaca
     9421 acatgccggc aaaactcagc agcacaaaca gcatgatcgc ggggatcggg ttcttgatcg
     9481 accaggagga gacgttcatc aggatgctcc ttatttggta gcttgtggcg cagatgctgc
     9541 gctggtcgcg gcctgatttt ctgtcgcagt ttcgctgatg cgaacgaggt cgccatcgtt
     9601 gagaaagccc gcaccgtcaa ccacgacgcg cgtatctggc gccaggccgg agaggacctc
     9661 gacccggtcg cccgcgagcc ggccggtcac caccttgacc tggctgacgc ggttgtcggg
     9721 tgccaggcgg aatacaaagt tgaagccgtc gcgcacgacg atggcgcgtt gcggcaccgt
     9781 cagcgcgtcc gaggcgccga ggttgaactc gccgcgcgca aacatgccgg ccttggcggg
     9841 cgaattcttg gcgggcagga tgggcagatc gacatacacc agcgccgagc gcgtctgcgg
     9901 atcgaccgtg ggcgcaatca tgcgcacgcg gccctggatc tcgccaccgc tggcgggacg
     9961 cacgaccgcc gtggtgccgg ccgtgatgcg gccgagttcg gccgaggtca cttcggcgcg
    10021 ccactcgagt cggcccttgc ggatgagccg aaacaactcg gtgccggcgc tgaccacggc
    10081 gcccacggtg gcggtgcgcg cggagatcac gccgctgtcg ggcgccagca cctgggtttg
    10141 ccggccgcgc agctgctgcg cgcccagcac ggcctgcgcc gcctcggcac gcgcgcgggc
    10201 ggttttctcg gtcgtcaggt actggttgat ctgctgggtg ctcagtgcgc ccgagttctg
    10261 cagcgtgcgc gcgcgttcgg cattggccgc ggcgtcggcc gcgctggcct gcgcttccag
    10321 caggctggcg cgcgcctgcg ccacgtcggc ctgcacgctc tcggttgaaa aggtcgccag
    10381 cacctggccg gccttgacca cgtcgccgac attgacccgc acctcggtca ggcgcaggcc
    10441 gttcgattcg gaaccgatga tggcttcctg ccaggccacg atgttgccgt tggcggccag
    10501 cgtgaggggc aggcgggccg gctgcggctg cgcggcggtc acggtcagcg ccggcttggg
    10561 cgcggccttg gcgtctgcag cggcgtcccc ggatttcccg gaagacagga aaaagaaggc
    10621 gcctgcggtc accacagcca caacgacggc gatcacggca acgggtttga gtttgaattt
    10681 tttcatggtg ttcttggaat caaacaggga gggtcaaaag gagaaaaaga agagctcagg
    10741 gccgggcgga tgcggcctgg gcattcgacg atgcggaggg gccggccatg gccggcgtcc
    10801 agccgccgcc catggcgcgg tacagcgcca cccaggccga ccggcgttcg cgctgcagcg
    10861 tggccagggc ggtttcggca ccgagttgcg cgcgccgtgc gtcttccagt tcaaccaggc
    10921 tggccaggcc attgcggtaa cgcgcgtcgg tgccggtgaa ggacgcgcgg tatccggtca
    10981 ccgcggtctg ggcatcacct tcgcgttcgg cggtgctctg cagcgtgacc agggcttcct
    11041 cgacctcgcg cacggcttgc cgcacccgcg cgcggtacag cgcggcggct tcctcataac
    11101 gcgcctgcgc cgcatcgacg tcggccgcgc ggcggccacc atcgaacagc ggcaaggtca
    11161 gggccagcgg cccgatggac caggtgtcga gtgtggtggt cacgccgccg gcgcgagaac
    11221 gcgagctgcc gatggcgccg ctcaggctca ggcgcgggta acgctctgcc tgggcgctgc
    11281 cgacgtcggc gctggcggcc gcgacctcgc gctcggcgtt gaacacgtcg ggacgctgtg
    11341 cgagggcgtt ggccggtaca tttgctattg aaatcatagc tggttgcgca ggcagatcgg
    11401 gcgctgcagc cactttttgc ctgatgtccg gctcggccag gccggtcagg gccaccagcg
    11461 ccttcaggtc gagctcacac tgcgcgcgct gctgcgtggc ccggccgctg gcctcggccg
    11521 cactggcgcg tgccagcgcg tcggtggccg gtgcggtgaa accggcgccg gtgctcagcc
    11581 ccgacaggcg cgccgtctcg ccgcgcgaac tggcatcggc ccgtgtcacc gcttcgagct
    11641 ggcggcaggt gcgcaggctg tagtactggt tggccacctc ggccgccacc gacacacgtg
    11701 cgtcatgcca ctgcgcccgg gcgccggcca gacgttcgcc ggcggcatcg cggccggcca
    11761 gccggccgcc gaacacgtcg atttcccagc tcgcctgcag gccggtctgc gtggtggtcg
    11821 tgaccggcgg cgtgcccggc ccggtggact gctgcgaacg gccccggctg gcgctggccg
    11881 tggcgtccag cgagggcagc agggccgcac cggcgcccac ccgcgtggcg cgggcctgct
    11941 cgatgcgcga gcgcgccgtg gccaccgtcg ggctcaccgc ttgggcggcg gcgatcagtt
    12001 cgaccagcaa ggggtcgccc tgctgctgcc accattgggt caggtcggtc agggcgccgt
    12061 tgtgcggcag gccggcgctg ccggctggtg ccggcagggg ggcaaaccat tgcgaggggg
    12121 tgttggcaga caccgctgcc ggggggcttt gcagggcgca ggcgcccagc agcagcggca
    12181 ccagggcgcc ggcggtgaaa cggggttttc tcatggcttg ttctttttca cgggaagggg
    12241 ggactggcgc cgggcgcatt ccaccgcgac catggcctcg gcaaaatcca ccaggcgcgc
    12301 ggcccacagg tcgatggctt ggggcgtggc aatcagttgc ggccggaccg tcacgatcag
    12361 gtcgcggctg atgaaggtct gcatggccag cccggtgatg gaaaacgcca gcctgtgcac
    12421 atcgtcgtcg ggtttcgtga cgcccagatg gcggcccagc acttccacca gtgcagcgtg
    12481 cgccggcagg atgttgttgt cgagctgctc ggcccacagg ccggtcggtt ccagcatttc
    12541 gcgaaagtgc aggcgggtca gctgttgcac caggtcgccc agcttcaggg gctccagaaa
    12601 gcccccgata aaaccttcga gagcctgccg cagtgtcagg tgcgccggca ggaagcgtgc
    12661 aatgtcgtca cagggcttgc ccagcggttc ggtgaagacc gcgcggtaaa gccccgcctt
    12721 gtcgccgaag tagtagctga tcgcggcaag gttggcaccg gccgcgtgcg caatctcgcg
    12781 cgttgatgtt ttggcaaagc ccttttcggc gaacagtttc agggcactga gcagcaggtg
    12841 ggcccgcgct tcggcaccgt cggcgcgctg cttgcgggct tcgggcccgc tggcgggtgg
    12901 gtgggcaggc agggagggtt tcatgggccc gattggacca cataaatcaa acgatcgatt
    12961 taattaaaac aactgtttca atttgatgca cggcgggcag gacggactcc cgggagccct
    13021 gccctggcag gccgtttcca gaagccttca ggcgccggcg tggggctcgc cggcttcgaa
    13081 caccgtgcgc cacaccgcca ggatggcctc gcgttcggcc tgcagcgccg aagggaccac
    13141 ctgggttggc tcttcgttga ggcgcgcctg gtgttgcacc cggcgcagcg cgcggtaggc
    13201 gcgggccacc gcctggcctt ggcccgcagg cagcaggccg gcgacctcgg cacgctgcag
    13261 caaggcgatg ttgccgacgt tgtcggccag ctccgggtgg ctgcgcgact gcgacaacac
    13321 cagaaactgc acgacgaact ctgcgtccac catgccgccg gggctgtgct tgacgtcgaa
    13381 tttttctccg gttttttcgc ccttgatcgg atgggcggca cgcactttct cgcgcatcgc
    13441 gacaatctcg cggctcaggg acgggatgtc gcgcgccgcg ctgatcacgg ccagccgcac
    13501 ggcgtcaaaa cgctggcgca gggagtcgct gccctggaca aaacgggcgc gggtgatggc
    13561 ctggtgttcc caggtccagg cggtgttgct gccgcgctgc tgctggtaat tggcataggc
    13621 ctcgaaacgc gtgaccagca accccgagtt gccgttgggc cgcaaggcgg tgtcgatctc
    13681 gaacaggtcg ccctcgccgg tcttgatggt cagccagttg atcagcttgc gcacaaaagc
    13741 ggcatacacc tccgaggccc gctcgtcctc gtcgtcgtag acgaacacga tgtccaggtc
    13801 gctaccgtaa ccgagctcct tgccgcccag cttgccgtag gcaatgatgg cgaactgcgg
    13861 cgtctcgcga tggcggttct tcaggcgctg ccagcaccag tcggtcgtga ccttgagcac
    13921 ggcttcggcc agggcgctga ggtcgtcggc gacctgctcg accgtgagtt gaccttcgac
    13981 gtcccgcgcc agcgtgcgaa acagctcggc atggtgcgcg cggcgcagca ggttcaggca
    14041 gctttcctcg tcgtcctcgc cggtgcgacg cagggcggcc agccgctgct gcagttcctg
    14101 catgaaggcc gcggcgtcaa aacgttcgtg cagcatggcg tcgctggcca gctcatcgat
    14161 cacgcccggg tgctgcaaca ggtaacgcgc cggccatttg gctgcgccca gaaggcgcag
    14221 caggcgctcg tgcacggcgg gacgctccac cagcagcgcc agataactct cccggcgcag
    14281 cagcggctcg atccagtcaa cgatgcgcac cgccgcttcc tcgccaacct tgccttcgag
    14341 cagccactgg gcggtgcgtt gcaccagccg agtcaggcgc gtccgggcgt cgtcgcgcag
    14401 ccccagcacg cgcgggtgct cgcgccacaa ctccagccgg gcgcgaaatt tgtcgggcca
    14461 gtggtccgcg agtttttcca gcagctcgtc gaggttttgc ggtgcggccg gcgccgactt
    14521 gccgcagccc ttgcactcgc ttttctgtcc cagcagcgtg tcgaactcct gggcgaccag
    14581 ctcgcggtgc gcatcgagtt cgctcagaaa cgggcagcag ctgctgtaac ccagggtctg
    14641 ggcgatccag gccaggtcat catcgcgcgt cggcagcaca tgggtctgct ggtcgtcgag
    14701 gtactggatg cggtgctcga cgcggcgcag gaaatcgtag gcccgcatca aggcttcggc
    14761 cgtggcctgt gccatcagtc cggcgctcgc cacgcgctgc agcgcatcga ccgtcgggcg
    14821 ggtgcgcagc tcggggaact gcccgccgcg cacgacctgc agcagctgca cggtgaattc
    14881 aatctcgcgg atgccgccgc gcgacatctt cacgtcgttg gcgcgctcgg ggtggccggc
    14941 ggcccgtttg gcggcgtgtt cgcggatctg ccggtgcagg atgcgcagcg cgtcgaacac
    15001 gttgtagtcg aggtagcgcc ggaagacaaa aggcaggacg gacccgcgca tggcctgcgc
    15061 cgagccgttc cggatacatt cgagcggcgc caccacccgg ctcttgagcc aggcaaaacg
    15121 ct
//
LOCUS       DAIDFG010000039        14672 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_39_length_14672_cov_17.842717, whole genome shotgun sequence.
ACCESSION   DAIDFG010000039 DAIDFG010000000
VERSION     DAIDFG010000039.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 14672)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14672)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14672
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39_length_14672_cov_17.842717"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2492 a         4650 c         4842 g         2688 t
ORIGIN      
        1 gcgcagcaca cgcggccaaa tgaagtcccc ctccatcgcc cagcgaggcg agggaggggc
       61 taggggaggg agtggggagt ccgtcctact gtccaaagcg aaccaccggc gcagcgtgat
      121 gcctgcgata tggaccagaa aagtaccttg tccaccacag cgaaacccgg attgcaggcc
      181 aaccagggta ggcggaatca aacagcagca ggtttgatcg acttaaaccg acgcccagaa
      241 caccgcaaac cagtccttcg gatcggtcca gggtttgatc tgcgaaaaac ctgcctgtcg
      301 cagcagtgcc gacatgctgt ccagcgtgta tttgtacgaa ctttcggtgt gtatgcgttc
      361 acccatcccg aaacgtcgct caccctccgg ccagtgcacg gtcagtccgc agcgtgcctc
      421 caggtgcatc tcgatgcgcg actgcgcttc gttgaacagg gcgatgtggc gccattgatg
      481 cgggtcgaag tcggcgccca gcagcatgtt cacgtggcgc agcaggtttt tgttgaaggc
      541 ggcagtgacg cccagcgcgt cgtcgtaggc tgcttccagc gtggccttgt ccttgaccag
      601 gtcgatgccg agcagcaggc cgcgtgcagc gccttgtgcc gggtcggcga tgcgctgcaa
      661 aaatgccagc gcctggtccg gcgaaaaatt cccgatgctg gagccggggt agaagaacac
      721 ccggtcgtga tcctgcacct gtggcggcag gagaagtcct gccgaaaaat ccatgcccac
      781 gccaacaatg gccagctccg gaaaggtgtt ttgcacctgt tccgcagcgt cgcgcagaaa
      841 gtccaccgaa atgtccaccg gcacgtactg gcggggctgc aggtgcggga tcagccgcat
      901 ggctttggag cagctgccgg cgcccaggtc gatcaggcag gggcaggtga tgccggcgcc
      961 ggccagggcc gccgccatgt ggccgtgggc cgcgtcgaaa atgccggcct cggtacgcgt
     1021 cgggtagtac tcgtcgaggt gggtgatggc ttcaaaaagc tgcgagccca gggcgtcgta
     1081 gaaatatttg ggcgacacgc tcgctgccgt cgcgcggagt ccgtccgcca gttcacgcgc
     1141 agcgccgggc tggccctgat cggacgcgtt gtagcggtcg atgaattgcg gggacgcagg
     1201 cgcaagggca agccctggct gttgaagggg gaagttcaaa atgggttcag cgtgtgctgg
     1261 tgtgggttca gacaatttac ggcagggctc ggcctgcctt tgtaggactg tttcgctttg
     1321 cttgcaaggg ggtgattgcc ggttgattgt ccgtccatgc cagatcccct gcgccggact
     1381 gcgttgatca ggccctgcta agcttggtgt gaccatgtct tcattcccac acccgatggc
     1441 ctccaggcgt ctttttctgc agcgcgccgc atgtgccgcc ggcgcaccgc tgctgggccg
     1501 tgatgccctt gccgcgggcg aagccgctta tgccgtgtcg ccgtggcagg gcgctgtgcc
     1561 gccgttgcag gcggtcgaca cccagggcaa ggcctggcgc ctgtctgacc tccggggccg
     1621 tgcggtgctg ctcaatttct gggccacttg gtgcgagccc tgccgcgcgg aaatgccgac
     1681 cctgcagcag atcgctgact tctacggctc cgacaagctg ctcgtgctgg ccatcaattt
     1741 caaggaacac ccgacgcgtg ccatccagtt tgcggccagc accgggctga gcctgcccgt
     1801 gctgctcgat cctcagggcc agacggcacg tgcctggggc gtcaaggttt ttcccaccac
     1861 ggtgctggtg gaccggcagg gccggccccg gcagcgtgtg cagggcgagg tcgactggac
     1921 cggcgccgcc gctgctcagt ggatcgaggc cctgcttgaa agctgagccg cttcattcca
     1981 gaggcaggac gaggtccgtg gtgccggggc agacccagcg cgcaaactcg cgcgtggtgt
     2041 ccaggcagcc gccgtcgcgg tacacgccgc gcggttcgcg gtagcgctgc atgagggcca
     2101 ggatgcgttc aagctcgggc tcatcgtgct gcgtggcggc caccagcgtt tcgaccctgg
     2161 cttcatcgat gcgttcatgg ccttcagcgt cccggtacag cgcgttgcgc cactggtaga
     2221 tttccaggca tttgtcggcc agtgtgtacg gttgctcacg cttccagaag ttgctgaaca
     2281 ggccaccgcc gaggtagatc acccaggagc cttcataacc tgatgcctcg gccgaggctt
     2341 cgtccggatt gcggaaccag gtcctgtcgc ccggcacaaa ataatgcggg ggcagagggg
     2401 cctccatcga accctgttcg cgcaaaaaaa catcgtgaaa cgggcccgac atgatgggcc
     2461 gggcagtcca gagcgtctgc agttgcgcat acagggcggg gttgcaatgc gccagttcct
     2521 cggcaatgcc cagcagcatc acgtattcgg tggcgcggta acaggagaat gaatacagtg
     2581 tgccggagac ctcgggctgg gtcgccttgg ccagcgcggt gaccagcgac acgccgggcc
     2641 ggatgacaaa gccgatgtct tccttgtagg tccagcagtc ctgcggcctt tccgcctggc
     2701 tggtgtgaaa ggacaggctg gtcttgcggg ccgcacgcgc gatgttgtgg cgaatgcgga
     2761 cggcggacgc cagttcatcc acgctgggga attcaaaggc caccggcccc atcagcatgg
     2821 ccgccacgat ctcgcgcgtc aggtcggccg gctggttgtc ggtgtccagc tgcagccggt
     2881 gggccagcgc gagtgtgtcg tagccggggg cccaggcctg gccctgttcc gcactgaggc
     2941 tgatccggac gaagtggccg gcgtcatcgt cgccttccgt gctgcgcacg cagtgcgcca
     3001 ggtcctgcgc gtccagccgc gtcagcaccg cctgcagcgt cgccgccagc ccctggccgg
     3061 cggttccgcg tagcaggatg ccttgcggtc ccgctgcttg tttgcccgat ttgcccatgc
     3121 catcgtctcc ggcttttgtg tttcgttatc gtgggagcct gcaacgctgg tggcgcgcgg
     3181 ggcgccagga gcttgcccaa taaatataac cgaggcattt ctgccctcgt gaccggcagg
     3241 acgaaagccg ccataattgc cgctcggcgc aagctttctg ctttccgccg catggacatc
     3301 ccggtgcacc ttctcatgcc cggtcacgtc tgccaaccca ccttccggag ttctttttga
     3361 tgaccaccgt acgccgtacc ttcctgaaaa ataccgctgc agccgccatg gctgccgcct
     3421 tgcctgcaat gagtttcgcc caggccgcac cacgccgcct cttcgcgccc cagagcggcg
     3481 cctggcgcac cttcgacgtg acaacacggg tcgacctcgt caagcctcag ggtgccgcca
     3541 aggtctggct gccgattcca tcggtcaaca gcgactacca gcgttcgcag ggcaacagct
     3601 tttcgagcaa cggcaaaacc gacctgaccg aagatggtgt ggacggcgcc aagatgcttt
     3661 acgccgaatt cgacgcgggc cagacccagc cgtttgtcga gttgacgagc cgcgtgcaga
     3721 cccagggccg cgcggtcgac tggtcgcaca aaaccgcggt gcgcgaaggt gccgacacgc
     3781 tgcgttattt cacgcgcccg acgaagttcc tgcccaccga cggcatcgtg cgcacgacgg
     3841 cgcagaaagc catcgccggc gcgaaaaccg acgtcgaaaa aacgcagagg atctacgact
     3901 ggatcgtgac caacacctat cgcgacccca aagtgcgcgg ctgcggtgaa ggcgacatca
     3961 agaccatgct ggaaaccggc aatctgggcg gcaagtgcgc cgacctgaac gcgctgtttg
     4021 tcggcctgtg ccgctcggtc ggcattccgg cgcgcgatgt gtacggcatc cgcctcgtgc
     4081 cctcagcctt tggctacaag gagctcagcg gcaacccggt cagcctcaag ggcgcgcagc
     4141 actgccgctc tgaagtgttc ctcaagggtt acggctgggt ggcgatggac ccggccgacg
     4201 tggccaaggt catgcgcctg gaaacggcca gctggatcaa gaacaccacc gacccggtgg
     4261 tggcgccggt caacaaggcg ctgttcggtg gctgggaagg caactggatg gcctacaaca
     4321 ccgcccacga cattgccctg ccgaattcga aactcgagaa gctgggcttt ttcatgtacc
     4381 cggtggcaga agacgcaggc gggcgtttcg actcctacgc gccggatgtt ttcaagtacc
     4441 agatcactgc aaaagagatt taagccccca cgcttgctca ctgcgtgtag ccgctgcccc
     4501 ccgagggggc gctgcgcctg cggcccggca gagccggttc cgcggcccct gcttgactga
     4561 aaacgccccg ggaaagggtg gcgctcaggg gagctccagc cgccgcttgc tgctgaactg
     4621 ccggcgctcg cctgtgaacg gatcatcaaa ggcgatattt ttggccagta gttgcaaggg
     4681 gtagcggtaa tcgtcctcag gtgttgggtc cagcggcggg tagacccggt cattgacgat
     4741 gggcaggccc agcgcataca tgtgcacgcg caactggtgt ttcttgccgg tgaccgggct
     4801 caggccgtaa cgtgccagcg gcccgctgac ttcgagcaac tcgatgcggg tttcgctgtt
     4861 ggctgcgccg gccacttcgg cctggcggaa aaagggctgg ccttcgacga tgcggctggt
     4921 gcggacgcgc ggaaacgtca gctccgggcg ccacggtgca atcgcttcat accctttgct
     4981 gacctcgcgt tggcggaaca ggtcctggta ggccgcgcgc tcctgcgctt gcactgaaaa
     5041 cagcaccagc ccggccgttt cgcggtcaat ccggtggatg gggctgagtt ctgcaatgcc
     5101 cagccggttt ttcaggcgca ccagcagggt ttcctgcagg tagtggcccg agggtgtgac
     5161 cggcagaaag tgcggcttgt cgaccacgac caggtgctcg tcctggaaca gcagttcttc
     5221 gtcaaaggga atgcgggtct ccgctggcag ggagcggtag taatacagcc gcatgtggcc
     5281 gcgataggcg cgttcggcgc tgactttttc gccaaattca tcgacaacca gctcatcgtg
     5341 catgcgctgc agccacacct cgcgcgcgat ggcgggaaaa cgttcgacca gaaaatcggt
     5401 gatggtcggc cagtggcctg ccggcaggcc cacacaactc gggctgacac cgtcccgggc
     5461 cggcaagccg tgcagaccca gcctgggggt tttgctcaca agcgagtaaa caccacgtcc
     5521 cagacaccat gcccgagctt gatgccgcgg ttttcgaact tggtgagcgg ccggtagtcg
     5581 ggcttgacgg cataaccgtc ggcactgtcc gccgtgtttt tcagtagcgg ctctgcgcgc
     5641 agaacctcga ggatctgctc ggcgtaaggc tgccagtcgg tggcgcagtg caggtagccg
     5701 ccgggcttca ggcgggccgc cagtttggcc accagtggcg cctggatcaa gcggcgcttg
     5761 ttgtgttttt tcttgtgcca gggatcggga aaaaagatgt gcacaccgtc caggctgccg
     5821 ggcgccagca tgtggtccag cacctgcacc gcgtcgtgtt gcaggatgcg gatgttttcc
     5881 aggccctgct cgccaatgcg tttgagcaag gcgcctacgc caggcgtgtg cacttcacag
     5941 cagaggaagt ttttctccgg cagcaaggct gcaatctgcg ccgtggcttc gcccatgccg
     6001 aagccgattt ccaggatggt gggggcggcg cgtccgaacg cggcttcgac gtccagtggc
     6061 gctgccgaat aaggcaaaag aaacttcggc cccgcatctg caaaagccct ggcctggccg
     6121 gccgtggttc tgccggtgcg caggacaaaa ctgcggatgg tacgcagacg cgcggggtcg
     6181 ggtgacgcgc cttccggcgg gttggacggg gggtttgatg gcgggttggt ggtgggggat
     6241 tcaatcaccg cccaatttta gtcgcccggc tccagaaagc cacggcttgc tgtagccagt
     6301 cggcgggcgt ccgggcagac aagcctgcgg gaagaccgag tacctgcccg gcttcatcca
     6361 gcgcctgcag cggctcggtg gtgtccagtg cgctggcgcc gttctgtttc gagagctttt
     6421 cgccgttggc cccgagcacc agcggcgtgt gcaggtaacg cggcaagggc aggcccagcg
     6481 cgcgctgcag caggatttgc cgcgcggtgt tgtcggccag gtcttccccg cggacgatat
     6541 cggtgatgcc ctgcgcgcca tcgtcgacca ccacggcgag ctggtaggcc cacaggccgt
     6601 cggcgcgctt gagcacaaag tcgccgacct cctgcgcgac gtcctgctgc tgcgcgccta
     6661 gccggcggtc ctgccagcgg acaatctgct ctgctactga atcaggagct ggttgcgccc
     6721 gtgcaataag ggctgagggc cgatttggct ttatatgttc agtgaggaag cgccacgccc
     6781 gcgctggacg accctgcagg ccgtgacggc aggtgccggg gtacaccagt tccccgtggc
     6841 gtggccgcgg cttgccgcgg gcctccagtg cccgtgcaat gtcctgccgt gtgcaggcgc
     6901 aggggtaggc gaggtgggcc gcgatcaact ggtccagtgc ggcctggtag tggctgccgc
     6961 gctgcgactg gtacagcggc ggcgcatccg gttgcagccc gcaggcggcc agctgttgca
     7021 ggatcagcgt gtcggcgccg gtcacgcagc gcggcgtgtc aatgtcttcg atacgcacca
     7081 gccactggcc accatgcgcc cgcgcatcaa gccagctcgc cagcgcggcg acgagggagc
     7141 cggcgtgcag cggtcccgtg ggtgagggcg caaagcggcc gatgtaggcc cgcggctcca
     7201 cacccgtgct cttcaggcga tggccagcgc catttcgagg ccggagacaa acgcgtcttc
     7261 agcccgatgg ccgacacacc agtcaccgca gctgccgatg cgcgatttgg catcccacag
     7321 gtgggtctgg cccagcggtt cggtggtttg cgcataacgc cagcggtgca ccaccgcgtg
     7381 cggtggctca gcgcgtatgc cggtgacctc ggtgaaggcc ctgagcagct tggccttgat
     7441 gcgctcgtcg tcgtcttcca gatgccgtgt ggaccactcc gggctggcct gcacggtcca
     7501 gcgttcgatg gggctgcgcc cgggtttgga cgattcgcgc gccagccagg caatgcggtg
     7561 gtgggtgctg cgcgccgcat tccactgcgg ccccaggtgc ggcagcgtcg gctgctgccc
     7621 ttgcggaaaa gccagcatca gggtccagca gggagcaatg gtcacacccg cgagggccgg
     7681 catcagggcc ttgccctgct gggaactgag cagcagggca tgcgcttgcg gtgcgggaac
     7741 ggccagcacc acggcgtcga agccggaata cacctggctg ctggcacccg ggccttcggt
     7801 ctgcaactgc cagcgctcgg ggtgcagctt gtcgcgttcc atccggatga cctgggtttc
     7861 cagcatcagg aagccggcgg cagccaatgg ctgggcccac tgcttgagca gcgcgctcat
     7921 gccgggtgtt gccacccagt gggcttccct gggtggcggt gcggacgcca cgacacggcc
     7981 tgcctcgtcg aggatgcgca cggtgttggc gctccagggc cgcaccacac cggggctggt
     8041 ggccagtgcc ttttcgaagc gcgcatcacg cacggtgaag tactgcgtgc catggtcaaa
     8101 gccgccaaat tccgagttgc gcgtggccat gcgcccaccg gggccgcggt ttttttcgaa
     8161 caccgtcacg cggtggcccg cctgcatcag tgtgcgggcg caggcgatgc cggccatgcc
     8221 cgcgccgacc acggcgatat ggcgtggcac ggttgtggct gagggagcgg aacgggattt
     8281 ttttgtcatg tctttgtact ttttatggcg gggtgaaagg ggcggttggc ctgcaggtct
     8341 cggtggcggg ctttcccgga ttttggcagc acagcgcaaa catcaatgga aatcatgatc
     8401 acactctaca cgctggcgcg gtcggccggc cgaagattcg ccgcgggtca gggttttaca
     8461 cggcatggtc gcgctccaga agggcttttc gcgtcgcggg gctgcccagc tgcttgagcc
     8521 accactgcag tgcgcggccc tcgccgccgg gcggcacgcg gcgccaggca taactgacgg
     8581 ggatgtgccg ttctgcccgc tgcacgcgct tttcaaccag cctgccggtg gcgatgtggg
     8641 gccgcgcaag gtattcgggc agaaagccgc cgcccaggcc gcgcagctgc gcatcgagtt
     8701 tttcctgcat gcccggcacg gtgaaaatgt cctggccgcc cagcagcccg accgagacgc
     8761 cgcttccgcg ctgcaccgag tcggcgaccg cgaccgcccg gtgcggcatc agcatcgcat
     8821 cggtgatggg ctcgatggcc tgcgccagcg gatggtgcgg cgcgacggcg tagatgaaat
     8881 ggatgctgcc cagcggcttg ctttgcaccg acttgtggat gccgggctcg atggccacgc
     8941 cgatcgccag atcggcctga cccgaggtga gggcttccag cgtgccggac agggcttctt
     9001 cccgcagccg caggcgggtg ggcgggttca gtgcaaaaaa ggcttcgcac aactccatca
     9061 cggtggtgcg cgagatcaca ctgtccaccg ccagcgtcag ctggggctcc cacccggtgg
     9121 ccacacgttt gacgcgattc gccaccgcgt cgagttcagc cagcaggcgt gtgccttcgc
     9181 gcagcagctc cttgcccgcc tcggtcagtc tggcctgccg ggacgagcgg tcgaacagca
     9241 acacgtccag tgcatcttcc acctggcgga tgcggtaggt cagcgcgctc ggcaccacgc
     9301 cgagctcgcg tgcggcggcc gccatgctgc cggtgcggtg tacggtgccg atcaggccta
     9361 tggtttcggg ggtcagcgca tcgcgcacgc ggttgttcat tgagggattg attgttcaaa
     9421 taatttgaat gatgccatca aataactgca atgaccagtg ctgcgccggc ccctacattg
     9481 gaggcactgc aaacgcattg cacacgcacc ctgaaaggag ctcccatgct gaccccccga
     9541 tcttccagcg aacgcggcta cgccgaccac ggctggctca agtccttcca ttccttctcg
     9601 tttgccggtt attacgaccc ggcccacatg ggcttcggca acctgcgcgt catcaatgaa
     9661 gaccgcatcg ctgctggcaa gggtttcggc acgcacggcc accgggacat ggaaatcatc
     9721 agttatgtgc tcagcggcga acttgcgcac aaggacagca tgggcaacgt caagggcatc
     9781 ccgcccggtg acgtgcagcg catgagtgcg ggcaccgggg tgcagcacag cgagttcaat
     9841 catgccgacg gccagaccac gcatttcctg cagatctgga ttgagcccaa tgtcaccggg
     9901 atcccgccca gctacgagca gaaaagtttc cccgacggcg acaaacgcgg cgcgttgcgg
     9961 ctggtggcat cgcccggcgg tgcgcagggc tccgtgacga ttcatgcgga cgccagcgtt
    10021 tatgcgggcc tgttcgatgg cgaggagacg gtcgacatgg cgctgaaccc gcaacgcaag
    10081 acctatgtgc acctggtgcg cggggaactc caggtcaatg gccaggccct gaaggcgggc
    10141 gacgcgctcc agctggaagc ggaaggccgg ctacagctgt cccatggcaa ggacgccgag
    10201 gtactggttt tcgatctgac ggcctgaacg ccgaagtgcc gaagcggccc gggacttgca
    10261 cggccgggcg cttcggttca aactttttct ttcatcactc tcaacaaaaa aggactcacc
    10321 atgctcaact cattgcaaaa cccgctatcc ctccttggcc gcatcctgat cgccctgctg
    10381 ttcgttccgg ccggcttcag caagatcgcc ggattcaccg gcacggccgg ctacatcgct
    10441 tccaagggtg ttcctctgcc cgaactggcc gcggccgcgg ccatcggcgt tgagctgggc
    10501 ttgggcctgc tgctgctgat cggcttgcag acccgctggg cagcgcttgg cattgccctg
    10561 ttcacggtgg tgatcacctt catcttccac gccttctggt ccgtgcccgc cgagcaggtc
    10621 atgatgcagc aacaggcctt cttcaagaac atcgccgtgg tcggtggcct gctgaccctc
    10681 gccgcctggg gtgcgggtgc ctggagcgtg gacgccaagc tcaagggctg atcgcggcac
    10741 gccgcgacct gcccacaggc cggcttcagg gccggcctgt ttccatctct ttttctcctc
    10801 tcttcttccc ttcctttttc tgaacccaag gacaccacac catgaccaac gtcgtcgtcg
    10861 tttaccactc cggttacggg catacccagc gcatggcgca ggcggttgct gaaggtgccg
    10921 gcgcggatct gctggccatc gacgccgacg gcaacctccc cgagggtggc tgggaacagc
    10981 tcaaggctgc cgacgccatc atcttcggtt cgcccaccta catgggcaac gtgagctggc
    11041 agttcaagaa gtttgccgat gcgtcctcca aaccctggtt cagccaggag tggaaggaca
    11101 agcttgctgc cggtttcacc aacagcgcgg gcatgaacgg cgacaagcag ggcacgctca
    11161 ccacgctgtt cacgctggcc atgcagcatg gcatggtctg ggtcagccag ggcctgatgc
    11221 cgtcgaacag caaggccgcg cagcgcgacg acatcaacta cctggtgtcc tacagcggag
    11281 cgatcgcgca gtcgccttcc gatgccggcg ccagcgagat ggccgcgggc gacctggcca
    11341 cggcccgtct gttcggtcaa cgcgtggccg atgtggccgc aaaatttcac gcctgatccc
    11401 tttctgatcc cttcctcgat ccttttccac gaaaccacgc tggagtcacc gccatgcctg
    11461 ttgacctgac attgaccgga cacgacaagg acctgggagg cggctttcgc gtgcgccgcc
    11521 tgctgccggc cacgcagcgg caggcggtgg ggcctttcct gttttttgac catttcggcc
    11581 cggtcacggt ggccccggga gaatcgcacg atgtgcggcc gcatccgcac atcggcctgg
    11641 cgacggtgac ctacttgttt gacggggcca tcatgcaccg cgacagcctg ggctccagcc
    11701 agcgcatcga acccggcgcc atcaactgga tgacagccgg acgcggcatc gtgcattccg
    11761 agcgtgcgcc gcaggaactc aaggcaagca cctacgtcaa ccacggcatc cagctgtggg
    11821 cggccctgcc gcgcgagttt gaagaggctg cgccggattt cgtacacacc ccggcttcgg
    11881 ccattccgtc gctggtgctg ggcggcgcgc aggttcgtgt attgatcggc gaggcgtttg
    11941 gccagcgttc gccggtcagg accttctcga ccacggtgta tctcgatgtg cagctcgctg
    12001 ccggcgcggt gttcgaactg ccgccgttgg ctgatgagct ggcgatttac gccgtggacg
    12061 gcgctgtggt ggtggacgga gagccggtgg ctgcgcacac gctggtcgtg cttgcgccag
    12121 gcgcggtggc atcgatcacg gccggcgacg cagtgcggct gatggtgata ggcggcgatg
    12181 cgcttgatgg ccaccgcttc atgtggtgga acttcgtgtc cagccgcaag gagcggattg
    12241 tgcaggcctc gcgcgactgg gagtcgcaga ccatgggcca ggtgccgggg gatgcagaac
    12301 tgattcccct gccagagcgc cgctttaccg ccgcgtgagt cctatgcgtc ctatgcggcg
    12361 gcgtcgcgtg ccaggcgcaa accgctgaac tgccactgcg cgtccggtgg aaaaaagttg
    12421 cggtaactcg cgcgtacgtg gctttgcggt gtggcgcagg agccgccgcg cagcacgaac
    12481 tggttgcaca tgaatttgcc gttgtattca ccgaccaggc cctcccacgg cttgtagccg
    12541 ggataggggt tgtagttgga ctgggtccac tcccagacat cgccaaacat ctgggccgga
    12601 tgacccgcac tgcgctgctg cagcgggatc gggtggtagg cgccgccttc gacaaagttg
    12661 ccttcggccc gctggccggg tggggccgac acactgcgtg cggccagctc ccactcaaac
    12721 tccatcggca ggcgcgcacc ggcccagcgt gcataggcgt cggcttcgaa ataactgagg
    12781 tgacagacgg gcgtgtgcgg gtccacctgc accagaccct gcaaggtgaa gttgcgatac
    12841 gggccgttcg gcgtgtctgg catctgccag tacatcggca tcgtcgcccc gcggccctgc
    12901 acccagtccc agcccatggc cagccacagc tcgggacgcc ggtaaccgcc gtctgccatg
    12961 aaagccatca tctccccgtt ggtgaccagc cggtggccga tctcaaacgg cgccacatac
    13021 accttgtggc gcggcgtctc gttgtcaaag gcaaaggcgc cgtccatgac agggtcgaag
    13081 ccatgctcga gcagtccgcc ttcaaagccc atccagcgca gcggctgcgg gttgatgccg
    13141 gccagcggcc agtgcccggc ataggcgggc cgtcccgggt tgaaggacag ggcgtgtttg
    13201 atgtcggtca gcagcagttc ctggtgctgc tgctcgtggt gcaggcccag tgtcagcagt
    13261 tgcatcagtt cgtcttcggc aggacctgcc ggggtttgcc ggaggacggt ctgcacacgc
    13321 tcgtccacct ctgcccggta ggccagcacc tgctgcagcg tcggccggct gatcaggccg
    13381 cgttgcggcc gcgggtgcct gtcgccaaca ccgttgtaat agctgttgaa cagcaccctg
    13441 aagctggcat cgaaaggccg gaaatccttt tcaaaacgct ccaggacaaa ggtttcgaaa
    13501 aaccaggtca ggtgcgccag gtgccatttg acggggctgg cgtcgggcat ggactgcaac
    13561 tggcagtcct cggcgctcag gggttggatg agacgttcgg tttggttgcg gatctgttca
    13621 aagcgcagcg acaggccgga gatccctgcg gcggtgcgtc gggcagcggt gggtgtgggc
    13681 atggcggtca gagcgtggtg atgagataaa aagccggttc agcttcccac accttcggcg
    13741 agcgccttgt cagacgatgc cccgacattg acgcggggct gcagggccgg gtccagcgcg
    13801 cgtcgatcat caaagacaaa acacgcacca tcaaaatgcg cgtggccgtt ttcttcaaag
    13861 tatttcagta tgccgccgtc gagctggaag acatgcttga cgcccgcctc gcgcatgaag
    13921 atggcggcct tttcacaccg tataccgccg gtgcagaagc tgaccacggt tttgccttcg
    13981 agctcggcgc ggtgcgccag cagggcctgc gggaactcgg tgaacttgtt gatgcgccag
    14041 tcgatcgcac cccggaaggt gccaacgtcg acctcgaagg cattgcgggt atccagggtc
    14101 acgacgggac gcccctcacc gtcgtggccg gcatccagcc agcgtttgag cgtcgcggca
    14161 tccaccgcgg gagcgcggcc ggccgcgggc tggatcgccg ggtggttcat gcggatgatt
    14221 tcgggcttga tcctgaccag cagcttgcga aaaggctggg catgggacca gctttccttg
    14281 acctccaggt catggaaacg ggtatcgcgc cgcaaccatg ccaaaaaatc atgaatgtcg
    14341 gcgcgcgggg cggccaggaa caggttgatg ccttcttccg ccagcagtac cgtgcctttg
    14401 atggcgcggc tctgcagagc ttcctgcacc ccggcctgca gctcagccag gccttccagc
    14461 gccacaaatt tgtaggccgc aatgttcaga actttcatga tgccggaatt gtagggggat
    14521 gccttcaatt caagcagggg ccgcgggtcc ggctttgccg gcccgcaggc gcagcggccc
    14581 ccgaggggct ggagcctgcg gctccaaagc tgcttgagca gctttggaca ggcgacagag
    14641 gcgaagcgtc tcgcgagccg cggcctgcaa gg
//
LOCUS       DAIDFG010000040        14561 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_40_length_14561_cov_18.135185, whole genome shotgun sequence.
ACCESSION   DAIDFG010000040 DAIDFG010000000
VERSION     DAIDFG010000040.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 14561)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14561)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14561
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40_length_14561_cov_18.135185"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2543 a         4935 c         4624 g         2459 t
ORIGIN      
        1 agctgctgcg cagcgcggtc gaagcctcgc tgcaagccgg cattttcggc tcacccaccg
       61 tggtggtgga tggcgagccg ttctggggtg tggaaaacct ggggctggtg gaacagtggc
      121 tggacagcgg cggctggtaa gcgctcaggc tgcagagaaa acgaattcca ccggaaccgc
      181 gatcgcggcc agacgctcgc gcagttggcg ctccagcgca tcatcacgcg tggacgcaat
      241 cgccaccacg gcagacaggc tgccgccgcg ctcctcgccc cgcacggtgg cgccggctat
      301 gtcggccagc atctcggcca gtttgacctc gatggcacgc aggcgcagcg ccggcttgta
      361 gatcttgccg atggccgtga ccggcatcac ctcgatgatg gtcacacgtt tgggcgccgc
      421 gggccgctcg tacacatggg gcaccacgtc ggccagcagc gtggccgggt cgcaagacat
      481 cccaggcctg agcgtgacaa aggccacggg caactcgccg gcataggcgt cgggctcgct
      541 gaccacggcg cacagggcca ccgccgggtg cgccaggaaa gcgtcttcga cgaggccggg
      601 gtcgatgttg tgcgagccgc gaatgatcac gtccttggcg cggccggtca ggtggatata
      661 gccctgtgca tcgatgtggc caagatcacc ggagaccagc cacggaccct cgaacatgcc
      721 ggcgttgcgc gcagcctcgg tgtagcccgg gctcacatgc gggccgcgca gcacgatgac
      781 gccggtgtcg ccggtcgcgc aggtctggcc cagttgcgcc acgccgccct gccagggcac
      841 ggcacgcacc tcggtgtaag gcaggcgcag gccggtggag ccgggcacac gcggcgccgc
      901 cagcggctcg atggacacca ggcccgcgca ctcggtcatg cccaggatgt tgcgcaccgc
      961 aatcccgggc ttgcgctcga actgcgccgc cagttccgcg ggcagcggcg agccgccggt
     1021 ataggccacc ttgattgacg agatgtctgc atccaccggc aggttggcca gcgtcgcgag
     1081 aatggtcggc acgcaggcca gcgcggtcac gcgttcacgt tcgcagaact tccagtagtt
     1141 tttggtaaag gcggggttgc gcatgccact gagcgtgggc aggacctgag ccgcgccgat
     1201 ggccagcatg gacagcccgt agacaaaagc cccggcgaca tgaaacagcg gaaaaccgtt
     1261 gagctccacc gtgtgttcgt cgaagccgta aaaccgatgg gcaaaccatc cggtgtgcgc
     1321 ctcgttgccg tgggtgtgct gcgcaagttt gggggagccg gtcgtgccgc cggtgtggaa
     1381 gcaggcggca atgcggtcgg gcgtgagttg tgggtcaaac gccagcacgt cgccggcctg
     1441 cgccagcaga tcctggaaca ccggcacgcc cggcgccgcg ttgctaccta cgcggatctg
     1501 cagcaccggc aggcgccgga tcgccagcac tttctcgacc atgggccaga gcgggagctc
     1561 gtcgttggga ccgagcgcaa ccatcacctt ggcgcccgac gcttcgagca gggccgcaat
     1621 gtgttcgggc tgcagcaggt agttgatcgg acaggccttg cccgccagtt gagcgcccca
     1681 cagtgcaaat tgcgtctcgg gaatgttcgg cgccagaatg accacggcat cgtcaggccc
     1741 gacgcccatg gcgcggaaca ggttggccgc ccggtggatc tgcgccagca gctcggcgta
     1801 acgcacggcc acggccggct gctgcaggtc gccgctgggc aggtagcgaa acgcatcacg
     1861 gccggcaaaa cgccgcgccg cctgggcgat caggccgtag gtggtgcgcg ccgggatcgc
     1921 ctggtcataa ggcgtgcgct ccagcgcctc gatgtcggcc agcccccgga tggcggtcgt
     1981 ggtcatgcgg cggcagcttg ccccaagccc agcagccgca tggcgttgcc tttcaggatg
     2041 ccgggcatca cttccggctt gaaaccggcg tcttcaaaat ccttcatcca gcggtccggc
     2101 gtgatcaacg ggtagtcgct gccgaacagg atgcgatcct tgagcagcgt gttggcgtat
     2161 tgcaccagct gcttggggaa atacttgggg ctccagcccg acaggtcgat ccagacgttg
     2221 ggcttgtggg tcgccacgct gagcgcctcg tcctgccagg ggaagctggg gtgcgccatg
     2281 acgatctgca tgtccgggaa atcgatcgcc acgtcgtcga ggtgcatggg gttggagtat
     2341 tcgagccgca gcccgccgcc gcaacgcatg ccgctgccga tgccgctgtg gccggtatgg
     2401 aagatggccg gcatcttgta ttcggcaatc acctcgtaaa tcggccaggc catcttgtcc
     2461 tgggggtagc agccctgcac tgtgggatgg aacttgaaac ccttgatgcc gtgttcctcg
     2521 atcagccggc gtgcctcgcg ggcgcccatt tttcccttgt gcgggtcaat gctggcaaag
     2581 gccatcatca tgtcactgtt tttcttggcg gcttcggcga tttcctcgtt gggaatgcgg
     2641 cgccggccca tctgcgtctc ggcatccacc gtgaacatga tcagcccgat cttgcgctcg
     2701 cggtaatagg ccacggtttc ttctatcgtc ggccggcggc tgttcttgaa gtacttgtcg
     2761 gcggcgcggt cgtattcctc gccgtagttg tcgaacgggt tccagcagct gacctccgcg
     2821 tgggtgtgaa tgtcaatcgc aatgaggttc tcgtagtcca tgctgtctcc tggtggttcg
     2881 acttgttgca cacctcattc gggttaacac tgagaaatga ggcggcaaag tcaattgaat
     2941 tggttattgt ttataacaat aatcgatgca acttgcaaac ctcccatgag caccaaaaaa
     3001 acccccaccg acctccagct tgaacttcgc ggcgaccacc aggagatcgc cgtgatccgc
     3061 ctgagccgcc ccgccaaacg caacgccctc aacgacgggc tgattctggc gctgcgcgac
     3121 atgtttgaca acctgccggc cacggtgcgc gcggccgtga tccacggtga gggcgcccat
     3181 ttctgcgccg gcctggacct cggcgagctg caggaacgcg acgccggcca gggcatgcac
     3241 cactcccgca tgtggcacgc ggcgctggaa cgggtccagt acggcccggt gccggtgatt
     3301 gccgcgctgc acggcgccgt ggtcggcggc ggactggaac tggccagcgc ctgccacatc
     3361 cgcgtggccg atgcgtccgc gttttatgcg ctgccagaag gctcgcgcgg catttttgtc
     3421 ggcggcggcg gatctgtgcg tatccccaag ctggtaggtg tcgcccgcat gaccgacatg
     3481 atgctgaccg gccgggtcta caacgccgag gatggtgaac gcgcgggttt tgcgcagtac
     3541 ctggtgcccg agggcgaggc cttcgacaag gcgctggagc tggcgcagcg ggtcgccgcc
     3601 aatgccccac tgaccaacta cgcgctgatg cacgccctgc cccgcatcgc cgaacaaccg
     3661 gccgaccagg gctttttcac cgagtccctg atggccgcca tcgcgcagag cgcgcccgag
     3721 gcgaaagccc gggtcaaggc cttcctcgat ggcaaggcca acaaggtcag caaggactga
     3781 tcccctcccc agccccgaca agaaaagaca tgagcgagac aaaacccact tccgcccccc
     3841 gctaccggcc cctgaccttc ggcgtgacac gtatcgccgc tcgcgagggc gatggcggaa
     3901 cccgttacat caaggccgac cagcccctgg gcgactacgc ggcgcgcatc acggaccgct
     3961 tcctgcattg ggcacaaacc gtgccggagc gcagttttct ggcgcgccgg gtccgccagg
     4021 ccgacggcag cacgggcgac tggcagcacc tgagctatgg ccaggcgctg gccagcgcac
     4081 gcgctctcgg gcaggcgctg ctggaccgcg gcctgagtgt cgaccgcccc gtggtgatcc
     4141 tcagcgaaaa cgggctggag catgcgcagc tggcgctggg ctgcctgtac gccggcattc
     4201 cctactgccc ggcctcgccg gcctattgcc tggtcagcca ggactatgac aagctgcgcc
     4261 acattcttga cacgctgacc ccgggactgg tgtttgccac ggatgcggcg cgttacggca
     4321 aggcgatcca ggcggccgtc ggtgccgacg tggaagtggt gctgcacgaa ggttcgctcg
     4381 agggccggcc gacaacgtcg ctggcctcgc tgctggccac cacacccacc gcgcaggtgg
     4441 acgccgcgat gcaggcgacc gggccggaca cgatcagcaa gttcctgttc acctcgggct
     4501 cgaccaagat gcccaaggcg gtggtgaaca cgcaccgcat gtggtgcgcc aaccagcagc
     4561 agatgcgcca gtcgatgccg gtgctggccg acgagccgcc cgtgctggtg gactggctgc
     4621 cctggaacca cactttcggc ggcaaccaca acgtcgggct gaccctgtac aacggcggca
     4681 cgctgtacat cgacgacggc aaacccacgc cggcactgat gggcgaaacc ctgcgcaacc
     4741 tgcgcgaggt ggcgcccacg ctgtacttca atgtgccgac cggctttgag gcgattgcca
     4801 acgccatgaa gaccgacgac gttctgcgca aaaacctgct gtcgcgcgtg aagatgttct
     4861 tctactccgg tgcggcactg gcccagcccg tgtgggatgc gctgcacgag acccaggaac
     4921 gcgagatcgg tgaacgcatc gtgatgggca ccggcctggg catgaccgag tcctcgcctt
     4981 ttgcggtgtt cgtcaccaac cccaacgtca aggccggcca cctcggcgtt cccacacccg
     5041 ggctggagct caagctggtg cctgtcgacg gcaagaccga ggtgcgctac aaaggcccga
     5101 acatcacgcc gggctactgg cgcaatgacg aagccacccg cgagagcttc gacgaggaag
     5161 gctttttctc gacgggggat gccgtgttgt ggatcgacga aaacgacccg cacctgggcc
     5221 tgcgttttga tggccgcatt gccgaagatt tcaagctcgc gacgggcacc tttgtcagcg
     5281 tggggccgct gcgggcgaag atcattgccg ccggcgcacc ttatgtgcag gacgccgtga
     5341 tcaccgggat caacctcaat gaagtcggtg cgctgatttt tccgaccccg gcggtgcgca
     5401 agctggccgg cctgccggac gatgcgcctc tgcagcaggt gctggaaagc gaaccagtgc
     5461 agtcacattt ccagaaagtg gccaacacgc tggccgccgg tgccaccggc agctccagcc
     5521 gcgtggcccg gctgcacctg atggccgcgc cgccgtccat cgacaagggc gaggtcaccg
     5581 acaagggctc catcaaccag cgcgccgtgc tcaaacaccg cgcagcgctc gccgatgcga
     5641 tgcacggcgg cacgctgcct ttcacgatcc agccgcaaca ggacaagcca tgaatatcga
     5701 cggacaagct gcactcgtca ccggtggagc ttcgggtctg ggcgaagcca ctgcgcgaga
     5761 actcgcgcgg ctgggcgcca aggtcgcggt gctcgacgtc aacctcgcgc tggccgaaaa
     5821 ggtggcggcc gacatcggcg gcgtggcctg ccagtgcgac atcaccagca gcgacagcgt
     5881 ggctgcagcg ctggaaaagg ccacagccgt ccatggcccg gcgcgcatcc tgatgaacat
     5941 cgccggcatc ggctcggcca aacgtgtggt cggcaaggac ggccaggccg cgccgctgga
     6001 agactttgcg aaggtcatca ccgtcaacct gatcggcagc tacaacatga gccggctgtt
     6061 tgcggccgac tgcgtgaagc tggacccgct ggaagatggc gagcgcggcg tcatgatgtt
     6121 caccgcttcg gtcgccgctt tcgacggcca ggtgggccag caagcctaca gcgcctccaa
     6181 aggcggcctg gtcggcatga ccctgccgat ggcgcgcgac ctggcgcagc acggcatccg
     6241 cgtctgcacc attgcgcccg gcctgtttgc cacgccgctg atgaagcagt tgcccgaacc
     6301 ggtgcaacaa tcgctcgccg catcgatccc gtttccgccg cgcctgggaa aacccgaaga
     6361 gttcgcgcag ctcgcatgcc atatcgtcac caatacccac ctcaatggcg aagtgatacg
     6421 gctcgacggc gccctgcgca tggcgccgcg ctgaaacagg cgccatacgt tcccccttgc
     6481 aacaactcgt gatcacgtga tttttgacag cccattttta caaccggaga cctagcatga
     6541 cctttaccaa atccatcctc gccattgccc tggctggttc ggcggctggc gccgccctgg
     6601 ccgacatcaa cattggcgtc agcctgccgc tgaccgggcc gacctcggca ctgggtatcc
     6661 cgatcaagaa ctccattgcc ctgtggccca ccagcattgg cggggaaaaa atcaatgtca
     6721 tcattcttga cgacgccacc gaccccacca atgccacgaa gaacgcacgc cgcttcgtca
     6781 ccgacgacaa ggtcgacatc ctgatgggct ctgccgccac gccgccggcc aatgcggtcg
     6841 ccgccgtcgc cgcggaaacc ggcaccatgc acatgacctt tgcgcccatc gacgtatcca
     6901 tcggcaaggc cccctggtcc tacaacaccc cgcagtccac gctggtgatg tcgcacgcca
     6961 tcgcggccca catgaaaaaa accggcgtca agacggtcgg tttcctcggc tacgccgacg
     7021 cctttggtga gctgtttctg cgcgacatga agatcaagat ggccggaacc gacatgaagt
     7081 ttgtgtccga agagcgtttt gcccgcaccg acaccagcgt caccggccag gccctgaagg
     7141 tcaccgccgg caatcctgac gccatcctga tcgtcgcctc gggcagcggt gccgccatgc
     7201 cgcacaaggc gttgatcgag cgcggctaca agggcaagat ctaccagacc cacggcgcgg
     7261 cctcgcgcga cctgatgcgt gtcggtggca aggacgtgga aggtgcgttt gtcgtggccg
     7321 gtccgggcgt cgtggccgag cagctgcccg ccaaccatcc cagcaagcag gtggccatga
     7381 cccacgtgac ggcctgggaa aagatttacg gcccgaacag ccgcaacccg tttgcgggcc
     7441 acgcctatga cgcgtccgtg atcctgcaga agatcattcc catcgccctc aagaccgcca
     7501 agcccggcac caaggagttc cgcggtgcgc tcaaggccgc ccttgaaaac gccggcccga
     7561 tcgcgctgtt tcacggcacc gtgaactaca ccgcgaccaa ccacgcaggc tacaccgacg
     7621 acaccggcat catcatgcag atcgtcaatg gcgactggaa gctgcaggcc aaataagccg
     7681 gcattccggg cactcccggt cggggcggcc tctggctgcc cccgaccggc gcctaccgtt
     7741 tcacgggctc cctgaaggta tttatggatt tttcaattgc cagcatcctg ctggtcgatg
     7801 gcctgaccaa tggcgcgatt tatgcgctgc tggggctggc caccgtgctg gtgtttgccg
     7861 tgacccgcgt catcttcatt ccgcagggcg agttcgtcac ctatggtgcg ctgacgctgg
     7921 ccattttgca gaccggcaag gtaccgggca cgatctggct gctgatgctg ctggccggcc
     7981 tggccgcgct ggccgaggcc tggcaaacct ggaagcgtta ccggcttgcc ggcaccgcgc
     8041 tgctggccgg cctcaaggtg ctggtcgccc ctgtcgtggt gtcgctggtc acgctgtggg
     8101 ccgcgccgca gcagttttcg ctgctggtgc aggcgctgct ctcggtctgc atcgtcacct
     8161 gtttcgggcc gctggtgtac cggctggcct atcagtcgct ggctgatgcc agctcgctgg
     8221 tgctgctgat tgtgtcggtg ggcgtgcatt ttgcgctgac cggcctgggc ctgctgtttt
     8281 ttggcgccga gggttttcgc aacccgccgt tctgggacgc acgcttcagc ctgggggcgg
     8341 tcaacctgtc cggccaggcg atcatcattg cgctggcatc catggcgttg atcgtgatgt
     8401 tgtggctgtt ttttgagcgc acgctgtacg gcaaggccct gcgtgcaacc gcggtgaacc
     8461 gcctgggcgc gcgcctgatg ggcatctcct cgcaaaccgc gggccagctg accctggcca
     8521 tggcggcgct gataggcgcg ctgtcgggcc tgctgatcgg ccccaccacc accgtgtttt
     8581 acgactcggg ttttctcatc ggcctcaagg gttttgtggc ggccgtcatc ggcggcctgg
     8641 ccagctaccc gggcgcggcc ataggcgcgc ttgccgtcgg catcatcgaa gccttcggct
     8701 ccttctgggc cagcgccttc aaggaagtga tcgtcttcac gctgatcctg ccggtgctgc
     8761 tctggcgctc gctcaagggc gggcactcgg acgaggagca ctgacatgac cctccgtaaa
     8821 tggggcccgt gggtcctggt cgctgcactg ctgctggtct gtgtcctgcc cgtgccggag
     8881 ttctggatca cccagctcaa ctacatcggc atttccagcc tgaccgtgct gggcctgatc
     8941 ctgctgaccg gcgtcggcgg cctgacctcc ttcgggcagg cggcctttgt cggtatcggt
     9001 gcctacacca gcgcctatct gacggtgaac gcagggctct cgccctgggt cacgctgctg
     9061 gtcggcctgg gcctgaccgc ggcctgcgcc gtggtgctgg gctggatcac gctgcgcatg
     9121 tccggtcact acctgccgct ggccacactg gcctggggcc tgtcgctgta ctacaccatg
     9181 ggcaacctgg acgcgctggg caaatacgac ggcattctcg gtgtcaccgg cctgtcgctg
     9241 ttcggctggg agctgggcca ggggcgggtc tacctgctgc tgacctggtc gctggtgctg
     9301 ctgggcacgc tggcgctgat tcacctgctc gattcgcgcg tgggccgcgc catccgctcg
     9361 ctcaagagcg gctcgcagat ggccgaatcc atgggcatcg acaccctgcg cttcaagatc
     9421 ggcatcttcc tgattgccgc gctgtttgcc tcggtgtccg gctggctgtt tgcgcacttc
     9481 cagcgttcgg tcaacccctc gccgttcggc gtgcatgtgg gcatcgagta cctgttcatg
     9541 gccgtgctcg gcggcgccgg ccatgtctgg ggcgccatca ccggcgtggc gatcaccaaa
     9601 ctcatggccg accagctgca ggtactgctg cccatgctca taggcaccag cggcagctac
     9661 gagaccattg tgttcggcgt gctgctggtg ctcacgctca agtacgcgcc tgacggcatc
     9721 tgggcctttg tggaccgcaa gctgcccaag gccgcgcgca aggtggactg gcaggatgcc
     9781 gccccgctgc cgcaccgcgc caagccggcc gccggcgaga tcgtcctcca ggccagcaac
     9841 atccgcaagg agtttggcgg cctggtggca gtcaacgaca tcaacttctc ggtggcctcg
     9901 ggccagatcg tcggcctgat cggcccgaac ggcgcaggca agtccaccac cttcaacctg
     9961 atcaccggtg tgctgagcct gacacgcggc agcgtgacct tccgcggtga aaccatcagc
    10021 agcctgccct cgcgcgaaat cgccaaacgc ggcatttcgc gcaccttcca gcacgtcaag
    10081 atgattgccg acatgacggt gctcgaaaac gtggcgctgg gcgcgcacct gcgcggcagc
    10141 aagggcgcgc cgtcggccat gctgcggctg gaccgggcgg aagaacagcg cctgctgcgc
    10201 gaggccgagc agcagctgcg ccgcgtcggc atgggcgacc acctgcatga actggccggc
    10261 aacctggcca tgggccagca gcggctgctg gagattgcgc gcgcgctgtg caatgacccg
    10321 gccctgctgc tgctcgacga gcccgccgcc ggcctgcgtt acctcgaaaa gcaggcgctg
    10381 atcgaggtgc tgcgccagct caaggccgag ggcatgagca tcctgctggt cgagcacgac
    10441 atggacctgg tgatgcaggt caccgaccgc atcgtggtga tggagttcgg cacgcgcctg
    10501 atcgaaggca cgcccgaaga aatccaggcc agcccggcgg tacgcgccgc ctacctcggc
    10561 acggaacatt gaccagcatg accacccctc ttctcaccgt caacaacctc cacgccggct
    10621 atggcaaggc cgaagtcctg cgcggcatcg ccctgcaggc cgaagccggc agcatcatca
    10681 ccgtcatcgg ccccaatggc gccggcaagt ccaccctgct caatgcgctc atgggggtct
    10741 tgcctgccac cggcagcatc gaatacgccg gccgctccat catcggcctg ccgctggaag
    10801 aacgcgtcat gctcggcatc gccctggtgc ccgaaaaacg cgagctgttc ggcagcatgt
    10861 cggtggaaga caacctcgtg cttggcgcct ttcgccaggt gcgtctgcgc aacaaggcct
    10921 ggcgcgacca gatggaggtg gtgttcgagt tgtttccgcg cctgaaggaa cgccgcgcgc
    10981 aggaggccgg cacactctcc ggcggcgagc ggcagatgct ggcgctgggc cgcgccctga
    11041 tgtcgcgtcc caccctgctg atgctcgacg aacccagcct gggcctggcg ccgctgatcg
    11101 tgcgggaaat cttccacacc atcgaggcgc tgcgccagac cggcgtaacc accctgctgg
    11161 tggagcagaa cgcgcgcgcc gccctcgacg cggccgatca tggctacgtg ctggagatgg
    11221 gcgagatcgg cctgagcggc ccggccgccg acctcgcgaa ggacccgcgc gtggtggaca
    11281 cctacctggg cgcgtcgcgc aaaccggctg ccaccgcctg aggccatgga ctaccagccc
    11341 cgcctcgacg acatccggtt catcctgaac gaggtgctgg acgccccggc gcaattgcag
    11401 gcgctcgcgc cttttgccgg caccgatgcc gacctcatgg aacaggtcat cagcgaagcc
    11461 ggcaaattcg tggccggaca gatcgcaccg ctgcaacgca ccggcgacga gatcggctgc
    11521 acgtgggacc aggggctggt caccaccccg cccggtttca aggccgccta tgccgccttc
    11581 tggcaaggcg gctggccggc gctggcctgc gcgccggagg acggcggcca gggcctgccc
    11641 gcggtgctgg aggcgattct gtacgagatg ctcagcgccg ccaaccacgg ctggaccatg
    11701 gcgcccggcc tgttgcacgg cgcctacgaa tgcatacgcc accatgccag cgacgccctg
    11761 aaggcgagct accttgaaaa agtggcgacc ggcgagtggc tggccaccat gtgcctgacc
    11821 gaaccgcacg cgggcagcga cctcggcctg gcgcgcaccc gggccgtggg ccaggccgac
    11881 ggcagctacc gcctcagcgg caccaagatt ttcatctcgg gcggcgaaca cgacctgacg
    11941 gacaacattg ttcacctcgt gctcgcgcgg ctgcccgatg cgccgcccgg ccccaagggc
    12001 ctgtcgctgt tcctggcacc caaggtcctg ccggatggcc agcgcaacac cgttcattgc
    12061 gatcgcatcg aggaaaaaat gggcctgcac ggcagcccga cctgcgtgat gcgttttgac
    12121 gaagccactt gctggatcgt cggcgagccg ggccgcggcc tggcagcgat gttcgtgatg
    12181 atgaacgcgg cgcgcctgca tgtggccctg caggggattg gcctgctcga ggccgcgtgg
    12241 cagaaggccg atgcttatgc cgccgaacgc cggcagatgc gcgtgcccgg cccgcgcgac
    12301 aacaaggagc cggccgatgc catcagcggc caccccgcca tgcgccgcct gctggacacg
    12361 cagcgcgcgt ggatagacgg cggccgtgtg ctcgcctacc gcacggcgat ggagctggac
    12421 atcgcaaaac accatcccga cgccacccgg caggccagcg cgcaacgctg gtgcagcctg
    12481 gtcacccccg tgctcaaggc ggccttcacc caccaggctt tttacggcgc cagcgactgc
    12541 ctgcaggttt tcggcggcca cggctatgtg cgcgaatggg gcatcgagca aatcgtgcgc
    12601 gacgcgcgtg tgcccatgat ttacgaaggc accaacgaga tccaggccat tgacctgctg
    12661 gtgcgcaagg tgctggccga cggtggcgcc ggcatgaccg cgctgctgca ggacctgcag
    12721 accggcctgg atgccggcca cccgggcgac gccgacgtgt tgcgccgctt tgccgacctg
    12781 tgcaacctca cagccctgct ggccaaggcc agccagaccg acccggtgct ggcctacgaa
    12841 gccgccgacg actacctgcg cgcggtcgcc ctcgccctgc tggcctgggc ctggagccgc
    12901 atcgacaggg tcgcggacga cgccccgcgc tggcgccagg ccagcgccgc gctgcacctg
    12961 cgcgtgttgc ccgaattcga catgcgtgtc aaaacaattg aaggacaatg caaacctgtc
    13021 cccgtgctga actgaacccc atgagcgctc gcgatcttcc caaacctccc gacccggccg
    13081 ccacggccac ggtggaccag gtcgatacca gctacctgca aagcctgatc ggctacaacg
    13141 ccaggcgcgc ggcgctgtcg gtcatcgagg tgttcatgga acgcatggcg ccttatgacc
    13201 tgcgggtcgt ggatttttcg gtgctgtcgc tggtcacgca caaccccggc atcacctcgc
    13261 gccaactctg caccgcgctg ggcatcctgc cgcccaacct ggtggccatg gtcaacagcc
    13321 tggaaaaacg cgagctgatc gcacgcaagc cgcacccgcg cgacggccgg gccatgggcc
    13381 tgcacctcac cgccagcggc agcaagctga tgcgcgacgc cgaacacacc gcggcaacgc
    13441 tggaagccga cgtcgccgcc cggctcacgg ccagtgagac gaaaaccctg ctgcggctgc
    13501 tgcaaaagat ctacctgtga cgcccggttg cccccacccc taacgcattc gttccaactt
    13561 ttcaagccct tttggctgtg aagctacacc ctgcgggcgc aggcagctat aaaactagta
    13621 gcagtcggga atttgcgcta tattcgcctt ccaagcccat gcttgccgat caactctgac
    13681 aaaaatcgtg accaggagat gccccccatg agtgccaaag aaaatgccgc cgtcggacag
    13741 ctgtttgaac tgcttgaatc gcgttatgcc ttgcgtgtgt tgtgggcgct caaagacggt
    13801 catccgcaga ctttccggtt gctgcaggac agcgtgggca gcatcacccc gaacaccctg
    13861 aacacccgta tcaaggaact gcgcgccgcc ggcctgatgg gtcacggcaa cgacggctac
    13921 atcgtcaccg cccttggtgc cgacctgctc aaacgcctga acgacgtgca ggccttcgcc
    13981 gccaaatggt cggccagcca gaccaaggtc acggccgtca agaaaaaata aggcgctgcc
    14041 gcccccacca agacaggccc tgcgcctgtc tttttatttg gggcgggccc tttcaaggcc
    14101 ggatttaagg cataaaatcg ggcttcagcc cttttacagc ttgcgcgaac agctatttat
    14161 ttgatagcac cggccagcca gcagacggcg cccacccgcg cagcaccagg ctgcaattcc
    14221 ttgtcttgaa aagggctttg ctgccccaag ctgccctctt gtccttcaga acaaccgcct
    14281 tcgaccatgt ccgaccacaa acaacccgaa caaaatcaag acctgagcgg tgcccccagc
    14341 ccggaagagc tggaggccgc ccaggccgcc aacgaggccg atgcactgag cgcggcccag
    14401 accgaactgg ccttgctgca ggccaaaaac accgaactgg ccgacaactt cctgcgcgcc
    14461 aaggccgagg ctgaaaatgc ccgccgccgt gccgacgatg aaatcgccaa ggcgcgcaag
    14521 ttcgccgtcg agagttttgc cgaaagcctg ctgccggtca c
//
LOCUS       DAIDFG010000041        14414 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_41_length_14414_cov_20.547183, whole genome shotgun sequence.
ACCESSION   DAIDFG010000041 DAIDFG010000000
VERSION     DAIDFG010000041.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 14414)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14414)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14414
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41_length_14414_cov_20.547183"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2516 a         4365 c         4701 g         2832 t
ORIGIN      
        1 cgccagggac gcgccggcca gcgcccatga ggccagcaac aggggtacgc cgcggtgcag
       61 caggtggggg cggcgggctt ggcccgggga ggtgcatgtc atgaaggttg gtcgtgccgg
      121 gctgactgtt cttacaggaa tcatgcccac gtttgcacgg gtgtgtgcgc cggtccccac
      181 gcgcatctgg ataccactgc agtcgcgcgg taaatgcccg gtaaatctcc gtttccctta
      241 cacggccttc acaaagccag gccaacaatg gcctcactga atcacacaca agccttcaac
      301 aagcagtgat tcgggcgcag ctcccactgt cgcttgccga ccctggaatc ccttccagcc
      361 actgcaagct gcccaccggg ctgcgggtta ttcatccgca tcgcaggaga acctcatgaa
      421 atcgaaactg ttgaccgctt ccctgttggc atccgccttg ttcgccggtc tgggcatggc
      481 cccggcgatg gcgcaaggca ataacaacac ccccggcatc gaccaatcac agcagtcgat
      541 cagcgcacgc atccagcagg gcctggcgtc cggccacatc acgccttccg aggcgcaggc
      601 actgtaccag cgtgaccgcg agatcgaagc ccgcgaaatg cgctacaagg ccaacggcag
      661 tgccaatccg caggagcgcc agcagttgcg ggccgacctg ggcgccctga gcgccgacgt
      721 ggagcgcatg atggccaaca atgtttacaa cgcgcccccc gtcgctggcg gcaataccca
      781 cggcatcgac aggctggagt acaagatcag ccagcgcatc gacgagggtg tgcgcgccgg
      841 ccgcatcacc cagcgcgaag cgcgcaggct gcacagccgc gagcgtgtca tcgagcgcca
      901 tgaggtcgcc ttcaaggccg acggcgtcgt gacacagcag gagcgccggc aactgcgcag
      961 cgagctgacc gcgctgcgcg acgaggttga gcgcatgatg cgcaacgacc gccgcaacgg
     1021 ccgcggttaa gaccacggtt agtgcaggcc ccgggcgttc agagcgtcca gacagaaaag
     1081 ccaggcactg cctggctttt ttcgtgcttg gtgataaaaa tgaccgccag cccaacagtg
     1141 gtgggcgtaa gcagctatca aaaacggagc gaattcaaag ccggtgcatt tgaccgggag
     1201 ctcaggccac gtatttctgt tcgcgcagcc acgcggccag gcgttcggca aacaccttgt
     1261 agccctcggc attcgcgtga atctggtccg agcgcagctc gggccgtgac aacacatgcg
     1321 cccagccgcc ggcaaacagc ggcacgccgg tggcgctggc gacttcggcg tacacctcat
     1381 ggtctttcag cgcgccggtg gccagcgcca tcagcgccgg ctcgggctgc gcaatcagca
     1441 ccacctgcgc actggccgcg gccgtctgca cgatggacgt gagcgcggcc cgtgtgcgct
     1501 ccggcggtag cctgcgcaaa aagtcgttgc cgccaatcga taccagcacc aggcctggcg
     1561 tgttggcctg cagcagcgcc ggcagacgcg ccaacgcgcc ttcggccgtg tcgccgttga
     1621 tgcccgcgtt ctgcaccaca tggccggtca attgctcgag cacgggcgga taagcggtgc
     1681 ccgccggcgc gccataaccg aaggtcagcg agtcaccgag gcacagcaag gtggcgtcct
     1741 tgggcagtgt tttggcggtg ggttttgccg atttcgagca gccggtggct agcagcagcg
     1801 cgccggccag gccgccaagc tgtgcaggac cacgatcatg ggggaaacgg gataaggcgg
     1861 gatcaggtgg gccgaggcgg gacaaaacca atgaaatcag gcctttacag ccgttttcag
     1921 gggttcaagc tccaaccggg tcgggccacg ataaatcggg ccgtgtgggg cgatctggcg
     1981 ggccgtcttc ccagcccggc gggattgaaa taaccccctg acccgctcgg cttcaaagct
     2041 ctttaaagcc ccattgagga cgcttgacgc tggtaggcag gccgcagcga ctgcggcttg
     2101 gcctggtgtg gcggctggtg ggcgatgagc gggcatggcg gcaatgctac accggcaatt
     2161 cggaacacca cttccgaatt ggctaccaat ggcggctcca accaattcgg aagcgaagtc
     2221 catcttaatt gccctccccg ctgcctgtgg ataacttcgc ctcgaccagg tgaacctcct
     2281 caaaaagacc tggctggttg cgggcgcgca tctcggccag gacgcgcttg taaatctggt
     2341 aaatccgcac gtagctcagg tcgaattcct tcgccagttc ataggcgttt ccgcgctcca
     2401 tgcgctggta aatttcatgg tcgcgcttgc tcaaatagaa ctgggtgtcg gcgttgaaat
     2461 agatatgctg gccgcgccag tttttagcca gaaaatcggc cagctcgttg cccacgtcgc
     2521 aggccttttc cttgtccagg ccgtggacct tgatcaggtg tagcgcggct ttctcggcca
     2581 tgtcggccag caggtgatgg cgcctgatgg tggattggtt atcgctgtga tctgtgctca
     2641 ttttgtggcc gctcctgtgc gtttttgctg ataggtcaaa gcctgggaaa ttcgctccag
     2701 gtcgcggatc gggcaccact cgaaaaactg tggcgcttcg tcgccgaaca tctgtttggc
     2761 gatgccgtcg gcataggtgt cgggcaggcg gtccaggctg atcaactggg cgcgaatgcg
     2821 cttgaccagc ggcagcgcat cggcctttgg tgtgggcctg gatgggcgct ttggtttggt
     2881 cgaggccttt ggcttccagc cctgcttttg aaggtaggtc agcacgcgct gccgcccttc
     2941 atgcgtcagg tttttggagc tgtccacacc gccctgtgca accaggatgg cgcggtattc
     3001 gtcatcagga agattcagtt ccttcctggc gatctggatc ttgatgacgt cgctgttgcg
     3061 gtggtcgcgg gctagcttgc tcatggctcg acaatctcaa aatttgcgtg gaagttagcg
     3121 ttcccgtaga cgcgcgggct gtgacggcca ccttcaaaaa tgacccaatc gccagggctg
     3181 attttttgaa agccatgcga ggcgctgtgc acgccccagg tggacattcc agtaacacca
     3241 gagctgacaa cgatgtcggg cagatcaccg ggctggtgcc attgcatggc atgcaagtca
     3301 acgggccggg tgcggacggc gatgtagctc atgactgaac ctgtggcgtg aagggaccga
     3361 gcagctcgca gccgtcttcg agcagcgtga tgtgaatggg gcagaggtgc cggtctggcc
     3421 ccacctgatg ggtgtggtcc tgacacagat gtcggtcaca tgtgcggccg ttcccgatgt
     3481 cccaatcgca ctgaaagcgg ctaggtgcaa ggcaccgctg catggcggca ccgtcgctgg
     3541 tctgaacacc gatggctgcg ccgcagcctt cgggcagctt ggtgccgcgc atgtgcacca
     3601 ggctggtcat tcccttgaag cggtaccagg gcattactgg ctcccggtgc tgagtcgcgc
     3661 ggcccgcgtt tccacgccgc gcagcagccg cgcataagaa tcgtctgccg ctggcttgcc
     3721 ctcagccttc gcaatgcctt ggatcgctgc ggtgcaggct ttcaggtact ccgaatcact
     3781 gaggaaaccg aagtccttgc ggcatgcccg gtaccgggta tttatcactc gctgcaaatc
     3841 ggctggcacc ataaaccagt gttttcggca cataaaccga ccgctggctg tggcgcaggt
     3901 gcagccgatg gcgtcacagg cgcggctcat gattgccacc ctgcagagca gagccgcgcc
     3961 ttggctattg cctggtcttc caccaccaag atcgatgtgg cgttgtccag gtgaacttcg
     4021 gtcaccaaaa tatcgggtcg gccgccggtc ctgtgaaaca agtgcgacgt gagatgcgtc
     4081 actctgcgcg ccgctatatc gactgttcct ccagcctcgc tgaaagctgt gaaggtggcg
     4141 aacggctgtg gctgtggcac cttgaccagg tactgccaga tcccgatggc gctggtgatg
     4201 gagaaaccga tctgctgcag cagaaagaac caatgcccat agccgttggc gaaaacgatc
     4261 cagccagcat tgctgaaggc gaacaacacc cagccccagc ccgcgcgccg gcctttcaaa
     4321 gccagcagca ggctgcccag gagcgcgcag accgcgctga acagctcaag ccaggcatag
     4381 ggcgtctgca tgtgctcggc ggccttgttg agccacggcc gcgcggcctg gtacagctca
     4441 agccaataga ggtcgagcga caggctcatg ctgtgagcct cccgtagatg accaagtacc
     4501 agggcaccag cacaaagacg gcaacaaacg gcagatcgtc ccgcctgaac ttttcgcgcc
     4561 agccggtcaa cgcaaaggcg atcgagaaga tcaccggcac gatcagccaa cgttcatagc
     4621 ggccgtaaag gatgggcgcc cagatgcagg aaagcagcgc aacggcgatc agcaagccga
     4681 gcgttcctcc gatgtccgaa tcattgaagg aaacccaggc caccactgcg agcatgacaa
     4741 tgctcaagat cagcggaaga acggtccaca actgcagggt gatggtgaag ttcatgctgc
     4801 accgccttcg gtgcgcgcgg cgtgcaggcg ccagcgggtg acgccgtgat cgttaccgcg
     4861 cggcagggcg tccacgctga ccagggatgc gccgtagagc ttctcgccca ggcgctgagc
     4921 ggcctgcttt tcgccagccg tgcagctggc cttctggccg cgcacggtgc tggtgaggta
     4981 ggtgccgcct gctgagcgca ccgtaatgtc tacagtttca ccccagccgg ccttctggcc
     5041 gtgcacggtg ctgttgacgc cgtcggccag gaacatctca gggatctgag cctcgaccag
     5101 cgcacgcagc agcgccagcg gtatccctac aaagttcggc gtcaccgcgt cgttgcacat
     5161 cggattggtg aacgtgtcgc ggaaaggctc gccgcgcttg gcctcggcgg ctttgaagtg
     5221 atcggcgatc gcctgggcag cggcggtaaa tttggtgttc ataagacagc ctttaaagcg
     5281 gtttaaaagg ccccagggcc gaagccctgg ggcgggaaga tcagccggtt gcggtttcca
     5341 ggccgctggc gtgatcaagg aaacatcagt cgaaacgggt catgagccag gacaggaagc
     5401 cgattaacac gatcagcgcc aggccttcga tcaatccagc gtccatatca gcgtcctttc
     5461 agatatcggt cgggccgatc tggagcagct cgcggcgctg ctgagcagtc ggctcggcct
     5521 gctggtaacg ccaggcagcc aggtgtgtgg ggcagcggct gcaaacgcca gtgccgtcgc
     5581 ggtggttgac accgagccag ctgcagccgc cagggcaggc gcgcaggtct gtgcagccgc
     5641 agctggtgca ggtggtggcg ttcatgctcg acccgcctga acttcgcgca tgaaggccgc
     5701 cacttccttg tgaaggccgt ccagcatctg cacgcctgcg gtctccgaca tggtcggtgc
     5761 ggcgatggtg tgcagctgca tgttgagcac ggcaccggcg cctgcataga acatgcgctt
     5821 gctcttgcgc agctgcacct gggacgcgtt cgccggcatg gccgccttga gcagcagctc
     5881 gtactgctcg gctatggtgt tgaagctcat acgtccctcc ggtcagcgtg tttgaactcg
     5941 tgtgtgtcac aaacgctggt gcgtccgacc ggcatggtgt gacgcctgca gcggctgttc
     6001 gatgtctcga aatagacgcc cttcgcaccc tcgcggtctt cgtggtcgca gaggtagaag
     6061 ccgcagtttt tgcaggccgg ctggggcagc ggcttggcgg gcttccggta gttgaccgat
     6121 gcgcgcaggt tcgccaggtt gatggcggcc gttgatttgc ccgcgctcat atagccgcca
     6181 cgtccaggct gatggggaag tattcgcccg tggtggcatc gcgcttgtaa aagcggatgt
     6241 agggcttggt gctggccacg cgcatgctgt cgccgattgc ctgcatcgcc agtagccact
     6301 tttcgtcggt gatctccagg cggcgcaggc ccagcacgcg gccggtgctg atcttgccct
     6361 gctggtccac ctgaaaggcg ttgttgacca gcaccttgat gttcgcgttg ctgcccttgc
     6421 tccagccgtg gatgcattcg tcgatcagcg ccttggcggc ctgcaggcgc tcatcaaagg
     6481 tgatggtctc ctgcatctgg cgaatgatct tgtactcgcc atcgaagttg atcagtgtga
     6541 cgttgccctt tttgccgccg tgctgcacgt catagttcgc caggcttttg tcgatgaagt
     6601 cggagatggc ctgcatcgcc gtggttttga aggccatcaa gcgggcactt tcctgctcgg
     6661 cctgccggca gatctcgccc accacctggt ggcgcagctt gtcgatttcc ttgatcttgc
     6721 tgaccgggat caggctgccg ctggcgtcct tccagtagcc agggtgcgcg gtttgttgtt
     6781 cattcatttg gaaactcctg cagtggttga ttttttggaa gggctgggga cgcccatttc
     6841 gatatagagg tcgggggcaa agcggccctt catggcggcc acttggtcca ccgtggtgct
     6901 ggcgcgctcc aggagaaaga cgcaggtgtc gtcaaactcc ttttgtgtgg cggccatgta
     6961 gtagccgctg gccgggtagc cgcagacgcg gtgctggtcc atgcgcagtt caacaatgaa
     7021 gtcgcgcacc gcccgctcca gcgccggctg gttggtggcc tggcccgtga tgcgggcgac
     7081 caggtcgcgg ccgtggatgc cgttctcgcg gccgacatgg cgctgcagct cggcaagcac
     7141 ctgttccttg ttgatttgca gttgcatgga tggctcctgt tactggcggg tggtcggggc
     7201 tggcggatag tcggcctggc cgggtgccgg cacggtggtg cgcgcgggcc gctggctctg
     7261 cagcagcatc tggccgaaca cgatggcccc gcccagctcc agcagcgagg tgccgacctt
     7321 ttcaagctga ccgtgcgcct ggcccagctt catgtaggcg ctaaacagcg cgtcaagctg
     7381 caccagggcc ggcagatcct tgagcagctc ttccacccgc gcggcggctt cgacaacccg
     7441 cgccatcgac gcggccatgt cctggtcgtt gtgctgggtg ttcatgccag tgctcctggt
     7501 tgaccggcaa tcgccaggtc gggcagcaac atgcagtcgg acgtgaaggc ataaatgcgc
     7561 ttgctggctt tttcgtcctg ttcgtcctgt tcgtcttcgc ctccctggtt gcggctgtcc
     7621 aggaagccgc cagcctccag ggcgtccacg gcctgctgca cctcatacgg ctggaaggtg
     7681 ggccgccagt gatgcgtgag catggcgacc gtccaccagc cgccatcgcg ctgcagcatc
     7741 agccagagcg cgcgggtgtc ttgagtgatt gcgttaccca tgaatgacct ttcctggaat
     7801 gaagggccgg gcgcggccgg cctcgatgct tgggcagttg gcgaagtcca tcgcgccgtc
     7861 gcggcacggc gttggtggcg gcggctggta ggggtcgccc tgcatgacgt aggtgcggcg
     7921 cggcggcgtg acgcatgcct gctcggccgc aggcggcgcc ggctcctggt cgatgctttg
     7981 gcttggctgg cccgccttgt agtacacggt gccctcgtca cactcgcgcc agacaatgcc
     8041 cgcgtcccgc atgtagccca gccgggaacg cagccactcc gggttgggcg tgtggccggc
     8101 cgggccgttg ctgcggaagc gcgccgattc cttgaacact tcgtgcagct gctcgaaggt
     8161 gcggcggccg tgctctttca ggtgcgccag gatggcctgg ctttgcgggc tgagcctggc
     8221 gcccttgggt gctgattttt tgaattgcat gatcaggccc cccttgcgca catctgctgg
     8281 ccggccttca tgccctgcat gaaagcgctg tccagctggg cgtcgataga gaccagcatg
     8341 gcgatggccg tcgccgcgcc cagcaccatc aaggccacag ccatgaacag cgccagccag
     8401 cgcaaaaagc gcaacagccg cttgcgccgg gacggcacag tcggaatcac gacgcctgga
     8461 taaaaagcgt gccggttcat gattggccac cgtggaaggt caggaagtcc aggcgcgcat
     8521 cgaagacggc gatcttgcga gtgacgccat aaaagcgctg caggcggcga gccctgcgtt
     8581 tgatccagcc gagacgtttg gcctggctca tgtcatgtcc tttcggttcg ggcaggtttt
     8641 gcaggcgcgg cccagggcgg cgcgcatggg gttggtgaag gcctggggaa gcgcctggtt
     8701 gtcctggcag ctgcgcttgc tcaaggtgcc catcacgggg caccgcactg tctcggccat
     8761 gaactggccg cgtatgcgct gctccatcgt ctcgacgttg ccggggtatt tgtcccgcag
     8821 cagcgtgctg atcacggccg agctgacgtg cagttcggcg gcaaccttga cttgagagtt
     8881 gaagcgcttc actgcggcct gcagcgcggc cttcacgtcg gccggtaaaa cgctttgaag
     8941 gcgtgggcta ttcaaggcca tcgcaaacct cctgcggtgt ttccaggttg gcgaactcgc
     9001 cggtgttgcg gtcgaagacc accttgcggc gcgtgatggc cggcgcgtgc gggccggtgt
     9061 tctttgccag gcggtgcttg gcaggtgtgc cgggcttggc cgcgcgcacc gtggaaaggt
     9121 agccagcgcg cgccatgtgg gcgacatagc tcttggcggt ggatggcttg accaccaggg
     9181 tgcccagcgt ggccgccttg gcaatgtcgg tgtagtcgaa ggtggacagc accttcatgg
     9241 cgcgccacat cgcctcgtta ccttcgccct gggtgacctt ttcgcccttg gtgctgacgc
     9301 gcggggcatc gccctggacc tttgccagca cgaactggat gggcttgccc atgacgccct
     9361 tgctgacgcc ggtaccgcct acccgcttga ggtagccggc cttctccaga tcgtcaaagt
     9421 agtcatcgac cagcgtccag ctgaccatcg ggttgcagtg gtcctggacg gacgtcttgt
     9481 cgaagcgcac accaggcgcc cggctgagca gcagcatggc ttcccacacc cgctcacgcg
     9541 gcgttttaag cgcgcgtaac tccacgttga tggacttgcg gctcatcgtg cccccttgcc
     9601 gctgcgggtg cccagcatcg ccagcagggg cgtgtcggtg ccggcgcgct tgcactcttc
     9661 gcgcaaggcc ttgagttcgt tgtaaacgcg ccgtgccacc ccgcccgttt tggtcaccag
     9721 ggcgtcgatc acgtcctggg tgaacttcag ttcgccagca tggtgggcgg ccagtttctt
     9781 gacgtccagg gcgtcgcagg gctgggcttc cttccagacc agcacccggt catggaagcg
     9841 ctcatgccgc gagagcagct tgcgcttcaa gtcctgctca ccgatcagaa agatcgggat
     9901 cgtggtgttg tcatggatgg tgcggatgaa gtcgatggtt gacttctcgg cgatgtagtc
     9961 cacctcatca agcaccaggg ggcggcccaa ctgctgcaag cgcttgacga tctggtcgta
    10021 cagcatggag accggccact ggctttttgt ctggatgccc agctccaggc acagcagctg
    10081 cgccagcgtc ttggtggtgt cgaacatgcg gatcgacacg aacaccgcgt tgcggccctg
    10141 cgggtgcgcc aggtacatgg ccgcctgcgt cttgccgaag ccgggcgggc cgtacagcac
    10201 cgccatgtgc ggcgcgccga ggtcgtccac atcggtcacc tggttgatcg cctcgatggc
    10261 gaaggccacg ttggtgagct tggcgacttg ctgacctggt tgaaacaggt cggattgatc
    10321 attgcttttg tttgacatac actttctccg tctttactga ttgctggttg agacacttcc
    10381 tctggagccc gcccgcgtga cagcgctggt gggctttctt tttgttatcc ggtggcttcc
    10441 tccccgagcg ggtcggtgcc ttcgtgcagc tcatgccacg aggcgaattc cttgctggtc
    10501 tggtagctgt ctgaccagtc caggtcacgg gcgctgatgg cctcgccacg atccttgcgg
    10561 gcctgcaggc gcacccaggt gctgtagcgg ctgcttgggc tggccagggg cagtacggcg
    10621 gccggtgcgg tggcctgggc ctccattgcg gccagagcag cggcgcggtc ggcgtccctg
    10681 ctggtggtgt ccaggtgggc caataccgaa tcgatggccg gcgtggtgtg ttgcagcacg
    10741 cggggagcca ggcggtggac gttgcctgcg gcggcttcgg cttcgcgctc gcggtcgttg
    10801 tagatcgcct gcacggccgt gcgggtgagg cccttcttca ggcccttgcg cagcctgtcc
    10861 accatcggct tgatgttggt cgcctcgatg gcgcgggcct tcgcggccag ctcggcgcgg
    10921 ttgataccga gcattgagtg gtccagggcg cggcaaataa aggagccgtc catcgcaaag
    10981 acatacagcg cgcccaggtc aagttcatcc tgcctgcagc gcaccgtgct gccgacatgg
    11041 gcggccagct cgggggcggc aaaccaaccc ttgtccaggg ggatgccgcg cttgccgaca
    11101 acacgggtgc ccttgccggc caccgccatc aggaagatgt ccagcgcccg atcgtccatg
    11161 cgcaccacat gggtggtgtg gcgttcggcc atctcgttgg ggctgcagcc cagctctgaa
    11221 tgggtgcggt tgtggtattc gtccagccag ccgttgatca cgtcctgcag ctgcctggca
    11281 cccatgcgca gatccagccc gcccaggccg aagcgctggg caaagctctt gcccgaatcg
    11341 atggccttgc gctctgccac actcttgccg acaaaccctt ccagcatggg gaagaggtca
    11401 tgcatcatcg tgccgatgaa gcgctcgata tagggctttt gctccgggct gaaaggcgtg
    11461 caaaggcggt gttcgatgcc caggctggtc agggcgaagt catagtcctg ggcggtgtag
    11521 tccttgccgt tgtcggtttt gatctgctct ggcttgcccc aggcctgaat gcaaagccgg
    11581 gtgaccgcct tgaccgcgtt ggagctgctg gtcggtgcaa ccaccacctg ggcgcggcgg
    11641 gtgaacacgt cgatgctggc aatgatggcg tggcggcgaa tctcgccggt gttctggtcc
    11701 accaggttga aggccaggtc tgcgcgctgc atggcgtcgc caatcgtgct gtcctgctgc
    11761 cactgctggt tggggcgcgt gatgccgtcc atctgcgagc cgaaggccga gcggtatttg
    11821 ttcttgtagc cgtccggatt tttgaacatg agcagcgcgg gggcgttgct gtccttgaat
    11881 tcgcgcaacc agcgcttgag ggtgctggtt gatggtgtgc gttccttgcc cacctggttg
    11941 cggatcacgc gctgcatctg gcgggcggtc gggtcatgca tctcggccag ggccgcgatg
    12001 aaggtgtcat gcaagtcttc atcacgggtt aaagcgcttt cgcccttgtc cttgcgcggc
    12061 ttgcgttcca gcagcgcctc gatgccctgg gtgcgccagg cccggtacca cttgtccagc
    12121 gtcttggccg ggatggccgg gtacgcggcc agtgtgttgg cgtgcgcctc aatgtggccg
    12181 gctccccaca gctgcacaaa ctcctggata gccggccaga cggccgtgcc gcgcagcgcg
    12241 tggtatttct caaaccgttg aaacaggtcc agcttcggat tgcgggccgg gtcaaacccg
    12301 cccttggctt ccagcacgat gccgtacttc gccatcgacg tggcgcaggc ctgctcctgg
    12361 gcgatctgcc tggcgcgctt gccggcggca atggcgtcgg ctgccggctt gacggccccg
    12421 gccgcctggg ccgcgatggc ctgctgggtt tcgggcggca agtcggtgct caggtattcg
    12481 aagccgctgc ggccccggcc ttcttcacgc gggcggcaag cccagcctgc ggactgggcg
    12541 cgcatcagca cgccgcgctg tgttccgggc aggcctggca ggccggcaag ggaggatgct
    12601 gacagccact tcatacgcgg gcctctgagg cggatgcctt gcgctgcttg cgcggcatgg
    12661 tgatcttgat cttgcgggtc agctgaaaag cacgggcgcg gtcggccggg tcgaggtagc
    12721 gtgaaggcca gatgtcctcc ggcttcatgc caagcgcttt tgccaccagt tgctcggcgg
    12781 cgagccagcg gccgtacagc acacgcgaga tgtgggcata accattggct tgggcaattt
    12841 gccgcatgct caagttgcgc ttgtgaaggg ccgcaataac gtcagcggga tgcatatcct
    12901 cttgtgctga tgtcgttcta tttgtgttca tgcacatgat aataagcaca aaacaaagga
    12961 agtcaacgaa tttatgcaca agggtttgtg ctgatgcata aatcagtgtg aataattacc
    13021 aaaagggctc aacggaattg ccttttgccg aatgcgcaca gaaaatagtg acgatgaaca
    13081 caaatcaagc gcagccggag caaggcagga cagagccttc ttcgatcgag cccgccaagg
    13141 tattggtggc cgaagagccg gtcgcttggt atctgcacca tgccgctgcc gcctcggaaa
    13201 aaaccgagtt gaagacggcc cgcactgagg caaagggctc ctacatgcgc cgcttcggtg
    13261 agcgctgcaa aacggccagg ggcgtgaagg aaatcggcga ggttgccgaa gcgctgggcc
    13321 tgcaccgcaa caccatctac aacatcgagc gcggcgtggc gctgccagac gcctttgagc
    13381 tggaggtgat cgcccagctg tacggcacca gcgcggccta cttgctggag ggcgaccacc
    13441 aggagaaagc caaaggaccg gacgcggtgc gcaagaacct ggaggccgtc caggtcgatg
    13501 ggtttattta tgtgccgctg ttcgacatcc atgtctccgc cggtggcggc gccttcaatg
    13561 atgttgaggc ggtcaccgcc atgcggccgt ttgatgccaa ttacatccgc tacgaactgg
    13621 gcatctccca caaccagatt gcgatgacgg cagttgtcgg caggtcaatg gagccctggc
    13681 tgcggcccaa ggacaccgtg ctgacggatc tgaaagaccg cgaggctttg accgagggcg
    13741 tgcacattgt taggcttgac ggcgcgctgc tggtcaagaa cctgcagcgg ctgcccggca
    13801 aggtgctgcg cgtgagcagc tacaaccagg cctatgagcc gtttgacata cagggcacgg
    13861 aagaccgcga ccgtgacttc gaggtgatcg gccgggtgcg gtgggccggc gtgactttca
    13921 gctaacaggg ggatctatga aatcgtcttt aaaactggct ttactgttct gtgccctcgt
    13981 gggctcggca agcgcagcaa cgccagacga tgcgcgcaag gccctcgatg cggcatgccc
    14041 agggctcaaa acctacgcag cggatttgag gatcgatgcg ccggccaaag gggcggccag
    14101 cctggatgcc caacgcgatc ggaaatggag cgaggcttac accatcacgg tgcgcgtgaa
    14161 cgacaagcca tccggcaaac tgatcggcca ataccgcgcc caggggcaga gctgctactt
    14221 cgaggtggag gcggtcaagg cggctgaagt gacggtcgcc aagtcggcat gtttggctat
    14281 ttgcagagac aagccgtata gtccgggagg gttcgggtat ttcggtgttg acggcgccga
    14341 agaagtgaag cgctgagcta taaatttaaa ccaagggaaa aataatgagc gaaaacaaac
    14401 cgccaccacc gcca
//
LOCUS       DAIDFG010000042        14303 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_42_length_14303_cov_19.440904, whole genome shotgun sequence.
ACCESSION   DAIDFG010000042 DAIDFG010000000
VERSION     DAIDFG010000042.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 14303)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14303)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14303
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42_length_14303_cov_19.440904"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2305 a         4526 c         5007 g         2465 t
ORIGIN      
        1 ggcaaggctg acatggccat ggctggcggc accgatgcgt ccaaactgcc agcggcgctg
       61 gcttcggtgc agggctgggt cagcgaacgc gcctgaactt accatttgtc cgttcgccct
      121 gagcctgtcg aaggggcctc acaaggcgga caggcttcga cacgctcagc ccgaacggta
      181 aagaggctgc accgcattgc cagcaggggc cgcgatccgg cttcgccggt cgcaaggcgc
      241 cgccccctgc aagggggagg aggcgaaggc tccacggggt gtgcttatgc cacttcggcg
      301 atcagctcaa tctcgacgca ggcgcccatg gggatttgcg cgacaccaaa agcgctgcgg
      361 gcatggctgc cggccggacc gaacacctcg cccagcagtt cgctggcgcc gttggtcacc
      421 agatgctgct cggtgaagtc gccggtcgag ttgaccaggc tcatcacctt gacgatgcgt
      481 gtgatcttgc cgagatcacc gacggctgca tgcagcgtgc ccatcaggtc gatcgcaatg
      541 gcgcgtgccg cggccttgcc ttcctcggtg ctcatggtct tgccgagttg cccaacccag
      601 ggcttgccgt cttttctggc aatgtggccg gagaggaaaa ccagtttgcc ggtctgcaca
      661 aaaggcacat aggccgctgc cggtgtggag accggcggca gggtgatgcc cagggttttc
      721 agcttgtcgt agatggggcc ggtgggttgg gtcatgaaac gctcctgaag ggggtgggtc
      781 gaaaaaattg aacgaaatag gcgctgggcc cttattcgac gggcgaaagc agctatgaaa
      841 aagatagcta ttctgatgga cgaccagcaa ttctagtccg ggcgccgccg tccggcggca
      901 tcactcgggc tcgggcagct tggcgatggg ggtcatcgtc accgccacgt ccagcacgtg
      961 gcgcgcaccg ccgcgtatca cgccgcgcat cggcgagacg tccaggaaat cgcgacccgt
     1021 ggccagcgtg acatagtcct cgcccggggc gcggttgttg gtggggtcga agtccagcca
     1081 gacgcccggg ctttgtcctt ccgccagcgc caggccgccg gcctcgcttt ccaccgggca
     1141 gtacaccgag gcccaggcat gggaggcatc gctgccgatc aggcgcggct ggccgggtgc
     1201 gggcgtggtc agcaggtagc cgctgacata acgcgccggc acacccatgg cgcggcagca
     1261 ggcgaccatg atgtgggcaa agtcctggca cacgcctttg cccagttgca gcgcctccag
     1321 cgccggcgtg cccacctcgg tgctttcgct gtcatagacc atgccggtgt ggatgcgttc
     1381 catcaggtcc tgcgccgctt cgggcaaggg ccgcccgggg gtgaagctgg gccgtgcaaa
     1441 ttccgcaaac gcctcgtcgc gcggaatgta gggcgaggcg aacaggaatt cgcaggcact
     1501 gtcataggcg gcgccggcgc ggtagcggaa ctgctcacgc acctgctccc aggacaggcc
     1561 tgccggcgcg tcttcgggcg ttgcggcaag cgccagggtg gagacgatgc tgtcggcgac
     1621 caccgtcagc ttgtcgtgcg gcgtgtgcag cgagaaatag gtgcgggtgt tgccatacac
     1681 gtcggtccgt tcctgcagcc gtgccggctg cgggctgatc gtcagctggt ggcgcagcag
     1741 ttgctggccg gcgtggctgg ccggcttgag gtgcagcaca tggtgggcac tttcgacggt
     1801 ggggctgtac gcataacgcg tctcatgaac aaggtgaagc agcatggcgg gaaaaaggaa
     1861 aggtgcagcc ggcgccggga actcaggcgc ccaggctgtg ttcgtcaggg gccacgtggc
     1921 tgaagtagcg ccggctcagc gtgtccgaca gctcgaaggc ggcactgcag cacaactcga
     1981 gctgcgccag caggcctgcg tacggttcgc tgttcggcgc gccggcggcg gggatgcaca
     2041 ggtcgctgag cagccagctg ccggggtcgg gcagggcggg cgtcaggtcg ggcaccggct
     2101 gcggtgcacc cacggtgttt tcggccaggc gcgccaggcg gccgcgcagc gtctgggtga
     2161 cccagccgag cgagcgcggg ttgtcgcggt ccagcaccag caggtcaagc agcgcccgca
     2221 catcgcgctg ctgctggtat tgggcatgga aggtgatggt gctgtcgaac agtgcgagga
     2281 tggcgccgaa gccgccttcg tcgaaaacgg cgccggtttc aaagccgagg atcagtgcgg
     2341 acgccagcgt gttcagccgc tcgatgtggc gccccgtgct gagcaggcgc cagccgtcgt
     2401 cgcggctcat gcggtcggtt tgcgcgccgg tcatggcggc caggaaacca ctggccgact
     2461 ccagcgcggc cagcgcttcc accgacgagt attcgagcgc ctgcgacgcg gggtgttcgg
     2521 ggccgctgaa ggctttgcag cgtgagaaaa aatcagcctc ggcccgcatg atcacgtgcc
     2581 actgctcctg cgacagccgc tcgcgcaccg ccgaagccgc gcccttgagc gcacgcaggc
     2641 tgtagccgac gctggcggcc tgatcggtat cggccaggct ggcaatcagt gagcgttcga
     2701 acacgcggcg cgactgcgtc gcaggcggca ccgtgtccat caccaggccg tttttcactg
     2761 ccagctcgga cagccaggcc agcagcggtt gtgaagtctg gtattcaccg ttgagggact
     2821 gcagggtcag ctgggccatg cggatggcgt tttcggtgcg ctcggtgtag cggcccagcc
     2881 agaacaggtt ttcggcggcg cggctggtga ccgggggctt gtgccgcaca ggggtcgccg
     2941 ctcggggctg ggcgcccggt tccgccgcca ggctgacggc cgacggtgag ccgttgtcgg
     3001 tgggggagcc cgtggcggcg ccggtcgtcg ccttgcgcgg cggtgcagat tcagccagcg
     3061 cccacacatc ggcgctgctg ccgccttgct gcatggatgc aatcttgtcg ttagggccgg
     3121 ccagccgcgc gagcccgccg ggcagaacct gccaggagcc cgcgccgtcg gccaccgcaa
     3181 acacacgcag catggtcgag cgcggggcaa tccgctgccc ctgccaagtg ggggtctggg
     3241 acagcggcag gtaggcctgc acggtgtagt cctcgctacg ccggaccagg cgaccggccc
     3301 attcatcgag ttcgcggcgc gacaaggtct gcgcaatcac cgattcaatg cccgacgccg
     3361 gataggtgcc cttgatgaca cattccttca ggcgcggcag gaccgcctgc agcgcagcct
     3421 gctcgccgca ccaccaggtg gccagcgagg gcagggccag tggctcgtcg agcaggtggc
     3481 gcgacagtgc cggcagaaag cccagcagcg cgctggattc gagaaaggcc gagcccgggg
     3541 cattggccac cagcacgttg cctgcacgta tggcttgcag caggccgggc acgccgagcg
     3601 tggagtctga acgcagttcc agcgggtcca gaaactcgtc gtccaggcgc ttgagcaggc
     3661 cgtgcacggg ctccagcccc tgcagcgtct tgaggtagag cttgtcgtcg cgcaccagca
     3721 ggtcgctgcc ttcgaccaaa gtcaggccga ggtaacgtgc gaggtaggca tgttcgaaat
     3781 aggtttcgtt gtagggaccg ggggtcagca gcacgatgcg actttcctcg ccgcgcgcgt
     3841 gcgccgggct ggccgccttc atgccatcga ccagcgcctt gtaggaggcc gccaggcgct
     3901 gcaccttcag ttcgcggaag gcctcgggaa acagccgcga gatcgcgagc cggttttcca
     3961 gcaggtaacc caggcctgaa ggtgcctggg tgcgttgcga caccacccac cagcgcccgt
     4021 cggggccgcg cgccaggtcg aaggcggcga tgtgcagaaa agtgccgccg gccggcacta
     4081 cgccgtgcat ggcgcgcagg tagccggggt ggccctgcac cagcgctgca ggcagcaggc
     4141 ccagcgccag caactgctgg gggccgtaca cgtcggccat gatgcgctcc agcagccggg
     4201 cgcgctgctg cacaccggcg gcaatctgcg cccagtcctg cgcggacagg atcagcggaa
     4261 acaggtccag cgaccagggc cgctgcgggc cgttggcatc ggcataaacg ttgtaggtca
     4321 cgccgttgtc gcggatctgg cgctgcaggt tggcggtgcg gtggttcagg tcgtcaaaac
     4381 cgtcgccgcc caggaagttg aagaagcggc tccagtcggg tgccaggctg gccgccgggg
     4441 cagcggtggg gccggcaccg gacgctggaa cgggcgccgc gggggcaccc gccagctgca
     4501 gcatttgtgg ggtctggctt tgactttggc cctgcatctg actggccgcg ggcggggcct
     4561 tgtccagggt ccggccgtgc aactcgtcaa aatgaccgat ggaagccggc agcgccaacg
     4621 ctgctgccag gtcgccaagt gcttccagcg cggcggctgc gaaaagggag gggttgtttt
     4681 ccacggtggc ccaagtatgc ctttatgcct aaggaagcgc tgaacaagtc ccctgcggcg
     4741 cgcgatcagc cgggctcggg cggtctgcgg cgttgaattt cttgccaata gtcagctatt
     4801 ggctgcgaaa tccgccctgc attccatccc gatccggctg ccgcgcatcc gcaaggactt
     4861 cttcagcgct tccctaacgc gcgggcaggt ccgcggccta ttgttctggc ccggagcaca
     4921 cgatggcagc gcaagtggcc accgttgttg cagccataag acaatttatg ggcgatcccg
     4981 aggccagcgg ggacgcacct gtcaaaaacc gcgccatcct ccccatgcgg tgtacagagc
     5041 ccccagcacc agcactgcgc cgacagcctg gccggccttg acctgacctt caggatggcc
     5101 gcgcagccag tcccgggcac ccgctgcgcc cagggccagg gcgccataca cccccgcctg
     5161 tgtcaaggcg atgatcagcc ccagaaccac ggcctgcagc cagaccggcc cgtactcggg
     5221 ccgcaggaat tgcggaaaga cggcgagcat gaagacatag gctttcgggt tcagcaggca
     5281 ggtcagcagg gcgcggccga aagtcgccgg cagggagcga gagcgtgttt ccccgacctc
     5341 gcccagcgcc gaagcgccgc gccacagtga caggccgatc caggcgatgt acagcgcccc
     5401 ggccatcagc atggcgttga acgccgccgg gaacagcttg agtaccacgc caatgccgat
     5461 ggcgcccagg gtcacatgca ccgcgccgcc ggccacgatg ccggcgaccg cagacaggcc
     5521 ggaacggcgt ccgtccatca gcgaactggc catgacaaag gccatgtcca ggcccggtag
     5581 cacgatgatg ccgaacacca tggcaaaaaa cagccacagg tgggcggtct gggtcaaagc
     5641 gggcatgtcc atttcaagct tccgtgcgtg tgaacgacat gacccagttg ttctcccagc
     5701 tgatgccttc atcggccgag taggcctgtt cccattgcgg ggtgtcaccg cttgtgttgg
     5761 accagatgta gcgaacgcgc accggccggc cgtcatgaac gtctgtgcct tcaaaaatgc
     5821 cggtaccccc cgcaaaagcc ccgcgcacgg gaggctgcag gagaccgtca tgcggggcga
     5881 cccagtagat cacccactgc cgggcctgca ggtcaaacag gcgcagtgaa acgcccacag
     5941 gccccgcctc gttgcgcatt tcgtccgtgt tgccgaggcc gtccagcaga ggctggcagt
     6001 attgcgtggc ttcaaagctg tcccattcgg tgcaccctag aaggcgttgg cgcaggcgcc
     6061 tgttgtgcac ccgccagcgg ccgtgcagga aatcgaaatc atgccgcccg ctttctttgt
     6121 ggcgggcggt agcggcctgg atggcgatgc tcatggatat ttccttgtgt ctgaggccgg
     6181 tcatcggccc ctgaggaatt caatgtaaaa gcgtaatagg gcgatattgg ccctgattag
     6241 gttttaaact cgttcgcatg tattcgccca ccacacgcgt gctcacggtg ctggaactgc
     6301 tccagaccca tggccgcctc agtggcgccg aactcgcgcg gcggctggag gtggacggcc
     6361 gcaccttgcg ccgctacatc gccaggctgg aagacctggg tattccggtg ctggccgagc
     6421 gtggccgcta cggctgttac cggctgatgg ccggcttcaa gctgccgccc atgatgttta
     6481 ccgacgacga ggcgctggcc ctgtcggtcg gcctggtggc ggcgcgtggc ctgggcctgg
     6541 ccgaagcagc gcccgcggtg caaagcgcgc aggccaagct ggagcgcgtg atgcccggca
     6601 agctcaaaaa ccgcatccgg gctctgggcg agacggtgag gctggatatg gcccgttcag
     6661 tcgccccgag cgacaacacc gccttgctgg ccctgagcag cgctgcccac ggctgccagc
     6721 gtgtgcacct ggcttaccgt tctgccgatg gtgtgctcac cgagcgggat ctggaccctt
     6781 atggcctggc ccaccgcggg gggcgctggt atgcggtggg cctgtgccac ctgcgccggg
     6841 atctgcgttc cttccggctg gaccgggtgc tggaggtgca ggcgctcaat gccgtgttca
     6901 cgccaccggc agacttcgac gcggtgcaac atctcgcgct ggggatcgcc accttgccgc
     6961 gggccttcag catccaggtg ttgctgcgca ccgcgatgga aaccgcgcag gaacaactgt
     7021 ttgaaggtgt cggcctgttc cagccctgcg ccggtggcgt gctgttgcac gcgcaggccg
     7081 atgacctgga ctggtttgcg cgccagctgg cccggctgtc gtttgacttc gagattcgcc
     7141 agccggcgga actgcacggc gcattcaggg tctgcgtgga ccgtctgcgc gtactcgccg
     7201 ggggcgctaa cgcagatcca gcgtgaacgg aaactcccgg ctcggctgcg ccggctggac
     7261 gcgcatcttg cctgcggtgt ggcccatctg gaaaaagcgg ttcaggcgcc ggctttcggc
     7321 ttcgtacgaa ttgacgggca gggtgtcgta actcaggccg cccggatgcg ccacgtggta
     7381 ctggcagccg cccagcgagc gttccatcca ggtgtcgacg atgtcaaagg tcagcggcgc
     7441 atgcacgccg atcgacgggt gcagggccga gggcgggttc caggccttgt aacgcacgcc
     7501 agccacgtat tcgccgaccg tgccggtgct ctgcagcggc agggcgcggc cgttggcggt
     7561 gatgacgtag cggttgtcgt tgaggccggt gacatggacc tcgacacgtt cgagcgagga
     7621 atccacataa cgcacggtgc cgccggatga gccttcctcg cccatcacat gccagggctc
     7681 cagcgcgctg cgcagggtca gctcgatgct gttggccttg acctggccga tcatcgggaa
     7741 gcggaactcg aaatggggtt cgaaccaggc catgtcgaag gcgtagccgg cttcgttgag
     7801 ctcggtcagc acgtcggcaa agtccatgcg cacaaaaccc ggcagcaaaa agcggtcgtg
     7861 cagctcggtg ccccagcgcg tcattcggcc gtggtaaggc ttgttccaga aacgcgccac
     7921 cagcgcgcgc agcagcaact gctgcacgat gctcatgcgg gcgtgcggcg gcatctcgaa
     7981 ggcgcgcagc tccagcaggc ccaggcggcc ggtggccgag tcgggcgagt acatcttgtc
     8041 gatgcagaac tcgctgcggt gcgtgttgcc ggtgacgtcg accaggatgt tgcgcagcgt
     8101 gcgatcgacg atccacggcg gcatgtcctg gccgtatttt tcgcggttgg cggcgatttc
     8161 gcgtagcgcg atttccagtt catacagctg gtcgttgcgc gcttcatcga cgcgcggcgc
     8221 ctggctggtc gggccgatga acatgcccga gaacagatag ctcagcgagg ggtggttatg
     8281 ccagtaggca atcaggctgc ccagcatttc cggcttgcgc aggaaggggc tgtccggtgg
     8341 tgtggcgccg ccgagcacaa agtggttgcc gccgccggtg ccggtgtggc gaccgtcggt
     8401 catgaacttt tcggccgaca ggcgtgtctc gtgcgcgatc ttgtagagga actcggtgtg
     8461 ttcgaccagc tcgttccagt tgtaggccgg gtggatgttg acttcgatta cgccggggtc
     8521 gggcgtgacc tgcagcatct tcaggcgcgg gtcgcgtggc ggcgggtaac cttcgagcac
     8581 gatctgcagg ctaagggcct gcgcgctggc ctctaccgcg gccagcaggt ccaggtagtc
     8641 ttcgaggcgc tccagcggcg gcatgaagat gtagagcgcg ccggtctcag ccttcgcgcc
     8701 tttttccttg ggcgctgcct tgggtttgtc ttgggggcca ttggcgcgcg tcgggtcgcg
     8761 cacttccaca cacagcgcgg tgcgtgtcag ccaggcggcg gactcgccgg gtttgggcat
     8821 gcgggcagag gggtcggccg gcgcaatggc cggtgccgcc gtggccgccg gccggacgcc
     8881 cggtttggcg ccctggtcga agcgaatgtc cacgctggac gtggtgttcc tgaactgcat
     8941 caaacggctg gcttcgttgc gcgaagcgtt gcccgtcatg taacgcgcca tgtactgtgc
     9001 cagcatgctg gcactgttgt gccgggcgcc ggccacggct gcggagacaa caggtgcggc
     9061 cggagcgggc acgctcaggc tgcgggctgc gttgtagcgc tcggcgagcg tatctgcaga
     9121 cggcaattca tcccgcggcg caaacgggtc ggcctcggtc atgtagggga aatcggtttt
     9181 cgagacccag ggcaccgaat ccagaggcag gcggtaaccc atgggcgagt cgccgggcat
     9241 caaatacatg cgttcatcgc ggaaaaacca ggggccggtg gaccattggg gccctttgcc
     9301 ttttgcgctt tcatcacgaa ccttgagcgg cagcacataa cccaccaccg cgtccagccc
     9361 ctgcgagaac acgcggcgca ggcgcgcgcg ctccatcgca tcgtcgagct tggtgtcgaa
     9421 cgggtccaca ttgaccggca gccggcgctc gcgccacagg tagtaccagg tgtcttcgta
     9481 gcccggcgtg atgtgttggg cggacaggcc cagcttgccg gccagcgtct cggtgaaacg
     9541 gcgcgcatcg tcactggtgt agctgcaggg ttggcgttcg tcggtgaaca gcgccgggtt
     9601 ctgccagacc ggctggccgt ccttgcgcca gaaaatgctc agggcccagc gcggcagctg
     9661 ctcgcccggg taccacttgc cctggccgaa atgcaggaag ccaccgttgc cgtattcggc
     9721 gcgcagcttc tgcacgagtt cggtcgcgta gccgcgtttg gtcgggccca gcgcgtcgat
     9781 gttccattcg gccgcatcac ggtcctgcgt ggcgacaaaa gtcggctcgc cgcccatggt
     9841 caggcgcaca tcgccagcga gcaggtcctg gtccacctgg tggccgagct tgagcacatc
     9901 ggcccacagc gcgtctgtat aaggctgggt cacgcgcggg gattcgtaga tgcgctgcac
     9961 acccatgtgg tgggcaaatt ccacttcgct cttgtccacg ccgccttcaa tcggtgccgc
    10021 gctcgagggc tcgggcgtgc aggccagcgg gatgtggcct tcgccggcca tcaggcccga
    10081 ggtcgggtcc aggccgaccc agccggcgcc gggcagaaac acctcgcacc aggcgtgcag
    10141 gtcggtgaag tcggcctcgg cgccgctggg gccgtcgagc gacttgacgt cggccttgag
    10201 ctggatcagg taacccgaga caaaacgagc ggccaggccg aggtggcgaa acagctgcac
    10261 catgagccag ccggtgtcgc ggcaggaacc gacggcgagc tgcagggttt cttccggcgt
    10321 ctgcacgccc ggctccatgc ggatcgcgta cttgatgtcg tgctgcagct tctggttcag
    10381 cccgaccagg aagtcgatgg tgcgtttggg cgtgcggtcg atgccggcca gataactctt
    10441 gagcaacggt gtggccggct cggtggcaag gtagggcgcc agctcatgcg cctgttcctt
    10501 gctgtacttg aaagggaact cctcggcttc gggctcgagg aaaaagtcga acgggttgaa
    10561 caccgccatt tcgacgacca ggtccaccgt gaccttgaac tcgcgcgttg gctcgggaaa
    10621 caccagcctt gcctggtagt tggcaaacgg atcctgctgc cagttgatga aatggccctt
    10681 gggctccacc gtcagcgaat acgacaacac cttggtgcgg ctgtgcggcg ctggtcgcag
    10741 cctgaccacc tgcgggccga ggcgaaccag ccggtcgtat tggtagtggg tgatgtgact
    10801 gagggcaacg tgaatggaca aggcaatctc gcttttcgaa tatttcagga gctgtgttgg
    10861 cactatgccg cagaaacagc acgccggtcg caaccggcgg ctgaatagcg gttttaacgc
    10921 gatccctaca agcaagagct gcgccaaaga ccgacatggc tgtcagcgcg aggcctgagt
    10981 tctccgttaa gcacgggatc ggaaactgcc gggcagaaaa aaacaggggg gcgttttggt
    11041 gaaagtgatg tcagccgcga cgcctcccgg ggccaggctg ctgggcccgg tcctgctcgg
    11101 ttttgtggcg ggcgccgccc tgcagttgca gcagcgcggg ctgtgggatg ccggatggta
    11161 tgccggcatc gccggcgctg ctaccgtgct ggccggcctg gtggcggcgg taacctcccc
    11221 ggcgaaggcg gcctggtccg cgcgcagcct ggcgacactg gtgctcgcac tggcggcagg
    11281 gtttgcactg accggcctgc gcgcggctgc cttccaggcc ggtgcactgg ctccggcgct
    11341 ggaaggcagg gacatcgtgg tgacgggccg ggtgctggcc atgccccagc gcagcgacga
    11401 cggcttgcgt ttccggttcg cggtggagag cgcccggctg gacgggcagc cggtggttat
    11461 gccgccgcag ctctacctcg gctggtatgc aggttttggc agccgggaag ccgtggatac
    11521 cgaaagcctg gacgctcccg cggcgcttct tgccctgcag cgccagccgc aaaacctgcg
    11581 tgctggcgag cagtggcgga tgacggtgcg actgaaggcg ccgcacggca acagcaatcc
    11641 gcacggcttc gactacgaac tctggctgtg ggagcagggc ctgcaagcca ccggttatgt
    11701 gcgtgccgga ccgcgcgatg cgccgccgca gatgctggcc gacggctggc gccatccggt
    11761 cgagcgggcg cggcagtgga tgcgtgacgc ggtgtatgca cgcgtgagcg atcgtcaact
    11821 ggccggcgtg gtggcggccc tgctggtcgg cgaccagaac gccatcgaac gcgccgactg
    11881 ggatgtgttc cgcgccactg gggtggccca cctcatgtcc atctcaggat tgcacatcac
    11941 catgtttgcc tggctggccg cgcaggccgt cggctggctg tggcggcgtt cggcgcgctg
    12001 gagcccggcc ctgtgtctgg cggttccggc cgccagcgcc gcagcctggg gcggcctgct
    12061 gctggccgcg gcgtacgcgc tgttttccgg ctggggcgtg ccggcgcagc gcacggtctg
    12121 gatgctggcg gccgtggtgc tgctgcgcca gagcggcaaa cgctggccgt ggccgcaggt
    12181 gtggctgctg gccatggcgg tggtggtggc gctggatccc tgggccttgc tgcaggccgg
    12241 cttctggctg tcgtttgtgg cggtaggcgt attgttcgca gcgggctcgc acccgccgca
    12301 gcatgctaca aattcaggag ctaccggcgc ccgtggagaa agggctggag gccaaaacga
    12361 ctctcaaact tcgcgctgga cggccatgct gcccgtctgg ctgggtgctg cagcaaagtc
    12421 tgtgcttcgt tctgcccgcg agcaatggat tgtgaccctg gcgctggcgc cgctgtcgct
    12481 gctgctgttc aaccaggtgt cgctggttgg cctgctcgcc aacggcctgg cgattccgtg
    12541 ggtgaccttg ctcgtgacgc cgttgaccat gctgggcgtg gtgtgggcgc cggtctggga
    12601 tctggccgcc ggggcggttg cggccctgat ggtatttttg caatggctgg cgcgctggcc
    12661 gctggccagc atcagcgtgg cggccgcgcc gctgtggtgt gcggtggccg gtgtggctgg
    12721 cggtctgttg ctggccatgc gtctgccctg gcactggcgg gcgctgggtc tgccgctgct
    12781 gctgccggta ctgctctggc agccgctgcg cccgccggcc ggggagttcg aactgatcgc
    12841 cgccgatgtg ggacagggca atgcgctggt cgttcgcacc gccggccaca cgctggttta
    12901 tgacagcggg ccgcgctttt cgcgcgagag cgacgccggg caccgggtgc tggtgccttt
    12961 gttgcgtgcc ctcggcgagc gcgtggacac gctgatgctg agccatcgcg acagcgacca
    13021 cattggcggc gcgccggccg tgctggcgat gcaaccgcag gcggccctgc tcagttccat
    13081 cgaagacggc cacgagctgc aggcgctgcg cccggccacc cgctgtgtgg cgggccagcg
    13141 ctggcagtgg gacggggtcg atttcgaggt cctgcatccg gccgccggcg actatgcaac
    13201 gccggcccgg tccaacgcga tgagctgcgt gctgcgcctg tccaacggtc ggcgcagcgc
    13261 cttgctggcc ggcgacatcg agctggcgca ggagcagcgc ctggtggccg ccggcgcgcc
    13321 gctgcgggcc gacgtgttgc tggtgccgca ccacggcagc aagacctcgt ccagcgcggc
    13381 gtttctggat gcggtgcagc ccacgctggc gctggcgcag gccggttacc gcaaccgttt
    13441 tggccacccg gcgcaggcgg tgctggcgcg ttaccgcgag cgcgggattg ttgtggtcgc
    13501 ttccccggtc tgcggcgccg ccacctgggc cagccagcac ccggcggagg tccgctgcca
    13561 gcggcaggcg gcgctgcgtt actggcagca ccggatggac gcggcgccct aagcagaaat
    13621 ctgcacaaat agtgatcggc ctacgaacgg ctggcgcatt acttgctaag ctagctgcat
    13681 aacgatccat tcgtaaacct gctggagacc aagccgatga gccgccccat gtttgatgag
    13741 atgaatgcaa cggcgtccag cgtccgcgag cactaccagg cctaccagcg ctggctggcc
    13801 agacaaccag atcaggtcat gcaggccagg cgcgcagaag ccgaaatgat cttccgccgc
    13861 gttggcatca ccttcgccgt gtatggtgac aaggacgacg gcaactccga aaccggcggc
    13921 accgaaaggc tgattccctt cgacctgatc ccgcgcatca tccccgcgca cgagtgggcc
    13981 agcatggaaa aagggctgat ccagcgcgtc accgcgctga atcgcttcat ccacgatgtc
    14041 taccacgacc aggagatcat caaggccggt gtgattccgt ccgagcagat tttccagaac
    14101 gcgcagttcc gccccgagat gatgggcgtc gaggtgcccg gcaacatcta ctcgcacatc
    14161 agcggcatcg acatcgtgcg cgcgcccaac gcgcagggcg agggcgagta ctacgtgctc
    14221 gaggacaacc tgcgcgttcc cagcggcgtg agctacatgc tggaagaccg caagatgatg
    14281 atgcggcttt tccccgacct gtt
//
LOCUS       DAIDFG010000043        13845 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_43_length_13874_cov_19.149721, whole genome shotgun sequence.
ACCESSION   DAIDFG010000043 DAIDFG010000000
VERSION     DAIDFG010000043.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 13845)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13845)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13845
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43_length_13874_cov_19.149721"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2496 a         4592 c         4413 g         2344 t
ORIGIN      
        1 agaggccggg cggggcgccc ggagaccgaa cgccccgcac cgtccgcaga tcaggccgcg
       61 agcgagcgca gcacgtaggg caggatgccg ccgttgcgga aataatccag ctcgtccagc
      121 gtatcgatgc ggcagagcag ttccacgtcc ttcaccgaac cgtcggcgaa cttgatctcg
      181 gcggtgagca tctggcgcgg cttgaggtca ccctcgatgc ccttcagggt gaccatctcg
      241 tcgcccttca ggcccagcgt ctgccaggag gtgccctctt caaacaccag cggcacgatg
      301 cccatgccga ccaggttcga gcggtggata cgctcgaagg actgggcgat cacggcgcgg
      361 atgccgagca gcttggtgcc cttggccgcc cagtcgcgcg aggagccagt gccgtattcc
      421 ttgccggcga acaccaccag cggaacgccc tcggccttgt attgcatggc cgcgtcatag
      481 atcggcagct gcgcgccgtc gggctgatgc agggtcacgc cgccttcgac gccgggcacc
      541 atctggttct tgatgcggat attagcgaag gtgccgcgca tcatgacttc gtgattgccg
      601 cgacgggtgc cgtactggtt gaagtccacc gggcgcacct gatgctcgat cagatacttg
      661 cccgccgggg acgcctgctt gatggagccg gccggcgaga tgtggtcggt ggtgatggag
      721 tcgccgaaca gcgccatgat gcgggcaccc ttcacagaca ctgcatcttt ttggcctgca
      781 gcccccgtcc tctctgcgcg ggcagctaca ttttccatag cgaagtcctt gaagaacggc
      841 ggctcggcga tgtaggtgct gacgggccag gtgtaggcgg tgcccgtcac cccctggatc
      901 ttttcccaca gcttgccggg ctcggtcttg accttggcgt agttctcgcg gaaggccttg
      961 ccgttcatcg cgaacttcat gagtttgtag atctcgtcgc tggtcggcca gatgtcgccg
     1021 aggtagacat ccttgccgcc cttgcccttg ccgaccggct cggtcatcag gtcgcgcatc
     1081 accgtgccgg caatcgcata agcgaccacc agcggcgggc tggccaggaa gttggccttc
     1141 aggttgggat ggatgcgcgc ctcgaaatta cggttgcccg acagcacggc ggcgcacacc
     1201 aggtcgctct tgttgatggc ttcgttgatc tcgggcgtca ggtcaccggc gttgccgatg
     1261 caggtcgtgc agccgtaacc ggccagtgca aagccgagct tttcaaggta gggcagcaag
     1321 ccggtattgg tcaggtactc ggtgacaatg cgcgagcccg gcgccagcga ggtcttgatg
     1381 tgcggctgca ccttcagccc cgcttcaacg gcttttttgg cgagcaggcc ggccgccagc
     1441 agcacgctgg ggttggaggt gttggtgcag ctggtgatgg cggcaatcag cacgtcgccg
     1501 ttgccgatgg tgatgccgcc ggccttgggc gtggcgggcg ccgggacgtc cgcgtgggcc
     1561 gtctccagcg tggggcggtt cgcctccatc tcggccacca tgcgcggtgc acctgccggc
     1621 gtgggcggca cacctggcgt cgtgtcgggg tgcgcctgcc cggcctgcaa gagcagatgg
     1681 cgcgtggcca gcatggcggc cggctggttg aagccgttct cggcaatcgg cttgctgaac
     1741 agcgaggcaa actgggtcgc cacctggccc agctcgatcc ggtcctgcgg gcgcttgggg
     1801 ccggccaggc tgggcgtcac atcgccgagg tcgagcttga ccacctgcga gaaatcgatc
     1861 tggccaggca gcggcacacc gaacagcccc tgcgcacgga agtaggcttc gaaggcctcg
     1921 atctcggcct tggtgcgacc ggtgccagtg aagtagtcaa tggtcttttc gtccaccggg
     1981 aagaaaccca tggtcgcgcc gtattcaggc gccatgttgg cgatcgtcgc gcggtctggc
     2041 agcgccaggg tacgcgtgcc ttcgccgaaa aactcgacaa acttgccgac cactttttcc
     2101 ttgcgtaagg cctcggtcac acgcagcacc aggtcggtgg cggtaacgcc ctcgcgcagc
     2161 cggccggtca gctcgaaccc gaccacgtca ggcgtcagca tgtagaccgg ctggcccagc
     2221 atggcggcct cggcctcgat gccgcccacg ccccaaccga cgacaccgat gccgttgatc
     2281 atggtggtgt ggctgtcggt gccgaccagc gtgtcggggt aatacacgcc gtcggcacgt
     2341 ttgtgcacgc cacgggccaa atactccagg ttcacctggt ggacaatgcc gaagcccggc
     2401 ggcacgacac cgaaggtgtc aaaggcctgc atgccccatt tcatgaactg gtaacgctcg
     2461 ttgttgcgct ggaactccag cttcatgttc aggtccagcg aatttttctt gccatagtgg
     2521 tccaccatga tggagtggtc caccaccagg tccaccggca ccagcggctc gatggccttc
     2581 gggttcttgc ccagcttgat cgccacgctg cgcatcgcgg ccaggtcggc cagcagcggc
     2641 acaccggtga agtcctgcag caccacgcgc gagacgacaa agggaatttc ctcggtgcgg
     2701 tcggcattcg ggaaccagtt ggccagctgg cgcacatgtt cggcgctgac cttctcgccg
     2761 tcgcagttgc gcagcacgga ttcgagcacg atgcgcatgg acaccggcag gcgcgagatt
     2821 ttcgggtact gcttggccag cgcgggcaag gagtaaaacc tgccggtctt gcctgacgcg
     2881 gtggtgaagg ttttcagggt cttggcgaac tcgtgggcca tggcgaactc cttggtgtgt
     2941 ggttttcgct attgtcgccc cgccagattt ctctcaacaa ctgcggggct tagccgccgg
     3001 ccggatcggg caggtaacgc accgccagca cgccctcaat cgcccgcagg tggtccagca
     3061 ccgcgtcgcc caccgggctt tccacgtcca ccagggtgta ggccatgtcg ccgcgcgact
     3121 tgttgaccat gttgtggatg ttcaggccgg cctgcgccat ggcggtggaa atctgcccca
     3181 gcatgttggg cacattggcg ttggcaatgg ccacccggta ggccgactcg cgcgccatgg
     3241 ccacggccgg gaagttgacc gcattgacca cattgccatg ctcgaggtag tcgcgcagct
     3301 ggtcagccac catgatggcg cagttttcct cggcttcgcg ggtggacgcg cccaggtgcg
     3361 gcagcgccac gacctggggg tggtggttga gcgttgcaga agggaaatcg cagacatagg
     3421 cgcgcagctg cttcgcgtcg agggcggcca gcaccgcggt ttcgctgacc acgccttcac
     3481 gcgaaaaatt gagcagtacc gcagccgatc gcatgagctt gatgttgtcg gcgttgacca
     3541 ggtcgcgcgt ggcagccacc agcggcacgt gcagggtcac gaagtctgag tttttcaaca
     3601 cctcggcgac gctggccgcc tttttgacct gcgccggcag gctccaggcg gcatccaccg
     3661 tgatgtcggg gtcgtagccg agcacgttca tgccgagctt gatggcggcg tcggccacca
     3721 ggcagccaac cttgcccagg cccacgatgc ccagcgtgtg gccggccagc tcgaagccgg
     3781 cgaagtgttt cttgccctct tccaccgcct tgtccatgtc agccatggtg atgtccaggc
     3841 cggcgacaaa acgcagcgcc ggtgcgatgt tgcgcgctgc cagcagcatg ccggtcagca
     3901 ccagctcctt cacggcattg gcattggcgc caggcgcatt gaacaccggc acgccgcgtg
     3961 cactcatggc ctggaccggg atgttgttgg tgccggcgcc ggcgcgggca atcgcctgca
     4021 cgctgtcggg gaccaccatg gcgtgcatgt cggccgagcg caccagcacg gcgtcgggcc
     4081 gggccatgtc cttgccggtg ctgtagcggt ctgccggcag gcgcttcaag ccattggccg
     4141 agatctggtt gagtaccagg atgtcgaagc gccggccggc cgcgccagcg gcctcagcca
     4201 tggctggcct cgaactcctt catatacgtc accagcgcct gcacaccttc gagcggcatg
     4261 gcgttgtaga tcgaggcacg catgccgccg accgagcgat ggcctttgag ctgcaccatc
     4321 ccgcgtgcct gcgcaccctt gaggaaggcc tcgtcgagcg actcgtcctt gagtttgaag
     4381 ggcacgttca tcagcgagcg gtcttcacgc gccaccgggt tgctgtagaa cgaggtggca
     4441 tcgaggtagt cgtacagcag ggccgccttg cggcggttgt gcgcctccac ggcggtcagg
     4501 ccgcccagcg ccttgaggtg cttgaacacc aggccggcga tgtagatcgc ataggtgggc
     4561 ggcgtgttgt acatcgagtc gttgtcggcc tgctgcttgt agtcgaacac ggaaggcgtg
     4621 tggggaatcg cctggccgat caggtcctcg cggacaatca caatggtcag acccgagggg
     4681 ccgatgttct tctgcgcgcc ggcgtaaatc aggccatagc ggccgacgtc gatgggccgc
     4741 gacagcagat tcgacgacat gtcggccacc agcggcacct tgccggtgtc gggcgtccag
     4801 tggtactcca caccgccaat ggtttcgttc gagcagatgt gcacataggc cgcatcgggg
     4861 tccagcttcc aggtgtcgaa tttcggcacg ctggtgaagc cgccggcttc ggcgctggcg
     4921 gcgacgttga ccttgcagaa gtttttcgct tccttgatgg attttttcat ccactcgccg
     4981 gtgttgatga agtccgcacc ggcatggccg cgcagcaggt tcatcggcac gatggcgttt
     5041 tgcgccaggc caccgccctg caggaacaac accttgtagt tggccgggat cgccagcagt
     5101 tcacgcagat cagcctcggc ctgggcatgg atggagatga actccttgcc gcggtggctc
     5161 atctccatca ccgacatgcc ggaaccgttc cagtccagca tttcggcggc agcctggcgc
     5221 agcacggttt cgggaagggt ggccgggccg gcactgaagt tcaaggcgcg tgtcatggag
     5281 agggctttct aggagggaag gaagtcggtc ggacgacccg gtcggatcgc cagaacgcac
     5341 agcatacaag aaagtccggc gccagccctg cccttcacca gccgggccgc accacggcct
     5401 tgcgcagtcc gacccactgc agttcggcca gcaggcccac ggtcactgcc tggatggcga
     5461 taaagacctt gccagccaac gtgggcatca ccaggtcgcc cagcagcagc gccaggcagc
     5521 ccagcaccca gagaaagttg gcgccgatga gcagccagac cagggctgcg ggcacgaact
     5581 gcctcgtcgc gatgaaaccg atgcccgcca catagacaaa catcacccag cccgacacca
     5641 ccagcagggt ctcgggcagg cccagcaggc cagccaggaa accggccagc agcaactgca
     5701 gcgcgcccgt ggccgcgccg gtggcggcgt ccagccacaa gacacggcgc aaaaacaggg
     5761 gcgatgcggc cagggaggaa aaggatgcaa tagcagacat gaaaaactcc ttgagtaggt
     5821 aagttgaggg acaggggaca gcggcctgat tccgttgccg cagccggctg tccatcgctg
     5881 cggtcatggc gccatgattg ccgccggagt gcgcagcgtc gataacctcc caggtaatgg
     5941 catccggccg cgccggcctt acgatggcgc catgactgcc agccttcacc ccaccgcacg
     6001 gcctcacccg tccaccccac tgccctttgg cgagcacctg cgccactggc gccagcaccg
     6061 gcgcctgagc cagctggacc tggccagcga ggccgacatc tcgaccaggc acctgagttt
     6121 tgtggaaacc ggccgggccg tgcccagccg cgaaatggtg ctgcgcctgt ccgagcgcct
     6181 ggcgattccc ctgcgcgagc gcaacacgct gctggtggcg gccgggtacg cgcccatgta
     6241 ccgcgaacgc ccgctggacg acccggcgct ggcgtcagcc cggcaggcgg tggagctgat
     6301 tctcaagggc cacgaaccct ttccggcgct ggcgctggac cggcactgga acctgctggc
     6361 cgcgaacgcc gccctgccgc acctgctggc cggcgcggac cccgagctgc tgcaggcgcc
     6421 catcaacgtg ctgcgcctga gcctgcaccc ccgcggcctg gccagcaaga tcgtcaatct
     6481 cgcgcaatgg cgcaatcacc tgttcgagcg gctgcgccag cagatcgccg ccaccgccga
     6541 cccggtgctg gcggagctgc tggccgaact gcaggcctac cccctgcccg aaggcgccga
     6601 cgacacccac cttgaaggcg aacacctggg cgtggtcatg cccttccagt tccgcacggc
     6661 ctacggcgtg ctgagtttca tcagcaccgt cacgattttc ggcaccccgg tggacgtgac
     6721 cctgcaggag ctgatgctgg agaccttttt cccggccgac gcgctgaccg gcgaggtgtt
     6781 gcgcaagctg ctgtcgcccg cgtgatcgcg cggcggcggg gcttgacgca ccatgcagcc
     6841 aggagacaac catgagcgac aacatccacc accgaatctg cccgctgtgc gaagcctgct
     6901 gcggcctgga actcaaggtc agcgacggca aggtcatcag catccgcggc cacgacagcg
     6961 acgtcttcag cgccggctac atctgcccca agggcgtggc gctgaaagac ctgcacgaag
     7021 acccggaccg gctgcgcacg ccgctgatca agcgcgatgg gcaatttgtc cccgccagct
     7081 gggacgaggc ctttaccgaa atcgaacgcc gcctgccgcc gattcttgcg cagcacggcc
     7141 gccaggccgg ggccatcagc gtaggtaacc cgtcggcgca caagatcggg ctgctgcttt
     7201 atttctcgcg cctggccaag gccctgggtt cgcgcaatgt gttttcggcg tccacgctgg
     7261 accagatgcc caagcagttg tccagcgggc tgatgttcgg ccactggctc agcatcgccg
     7321 tgccggacct cccgcgctgt gatttcctgc tggtgctggg cgcgaacccg ctggccagca
     7381 acggcagcct gtggaccgtg cccgacttca agggcaaggc caaggccatg caggcgcgcg
     7441 gcggcaagct ggtggtgatc gacccgcgcc gcaccgaaac cgccgccatg gccgatgcgc
     7501 accacttcat ccggcccggt gccgatgtgt ttttgctgct cggcctggtg cacacgctgt
     7561 ttgacgaaaa gctggttcgg ctgggacgca tcgcgcccct gatccatggc gtggatgccg
     7621 ttgaagccgc cgtggaagcc ttcagtgccg aggtggtcgc gccgcgctgc ggcatagccg
     7681 ccgaaacgat tcgcagcctg gcacgccaac tcgccgccac accgcgcgcc gcggtgtatg
     7741 gccgcatcgg cacctgtacg caggaatacg gcacgctggc gagctggctg gtggatgtgc
     7801 tcaacgtgct gaccggccac ctcgacgagc ccggtggtgc catgttcccg aaagccgccg
     7861 cgtttgcgca caacaccgct ggccagccgg gcagcggccg cggcatcagc accgggcggc
     7921 ataccagccg ggtcagcggc gcgcccgaag tcttcggcga actgcccatg acctgcctgg
     7981 ccgaagaaat cgagacgccc ggccccgacc aggtgcgcgc gctgatcacc gtggccagca
     8041 acccggtgct ctcctcgccc aacggcgcgc ggctcgcgcg ggccctggac agcctggact
     8101 tcatggtcag cctggacatc tatgtcaatg aaacctcgcg ccatgccgac gtgatcctgc
     8161 caggtccctc gccgctggag gacatgcatt acgacgtggc tttccctcag ttctcctggc
     8221 gcaaccacgc ccgttacagc gcgcctgtgt ttgctgcgcc cgaagaacag ccggcggaat
     8281 gggaaaccct gctcaagctg gccgccatcg tgcagggcca gggcgcacaa accccggcac
     8341 gcgagatcga cgaccgctac tttgccgaag acgcgaaaaa gctgttcggc gagcaggccg
     8401 cagccgtgat cgatgccacg caaggcctgc atggcccgga ccgcctgctg gaactcgcgc
     8461 tgcgcaccgg cccctatggc gatcagtttg gccgcgagcc cgagggcctg accctggcca
     8521 aggtgcgggc cgcaccggac ggcatcgacc tgggggaact gcagccgcgc atgcccgagg
     8581 tgctgcgcac gccgtccggc aagatcgaac tggcgccgcc cgcgctgctg caggacctgc
     8641 agcgcgccgc gcaggccatc acgcagccgg tgccggcgat ggtgatcgtc ggccggcgcg
     8701 acgtgcgctc caacaacagc tggatgcaca acctgcccgt cctcgccaag gggccgatgc
     8761 gttgcaccgc gctggtccac ccggccgacg cgaagcgcct gggcctggcc gacggcgcgc
     8821 tggcccgcat cagcaatcag ggacgcagca tcgatgcgca ggtgcaggtg agtgaggaga
     8881 tgatgcccgg cgtggtcagc ctgccgcacg gctggggcca tggcctgacc ggcacccgca
     8941 tggcgctcgc cgcagaacgg ccaggcgtca acctcaatgc actgcttgac gaagactggc
     9001 gcgatccgct gtcgggcaac gccgtgctca gcggcgtgcc ggtggtgctg gaacccgttg
     9061 gcgcctgatt tttttaaaca aaacaaggct ccagcccttg ctggtctttc gcaagcagct
     9121 attgaataaa tagcagctca atctgcttgg ttgaccatct gaaaagtgcg gtgcggctca
     9181 ctcggccccc tgaccagtgg ccgccggccg gtcggcacgg cccccaggcg cagcggcggc
     9241 cccggtgcgc atcagcaacg cgcccagcag cgccgccatg gcggcaaaca cggcaccgag
     9301 caaaaaggcc agctggtaac cgccgttgag cgccgccagc ggctgcgcgc cagcgctcgt
     9361 caggtgaccg gtgcgcgcgg cagcggcgct ggcgagcacg gccagcccca gcgccccgcc
     9421 catcatgaag gcggtgttga ccacgcccga cgcaatgccc gactcgctgg gtgccacatc
     9481 gctcatggcg gccagcagca cgggattgaa ggccatgccc gcacccaggc ccagcaacag
     9541 catgcccggc aacacgtcgg ccacgaactg gccgcccacc ggcgcccgcg caaacagcgc
     9601 cagcccgagc gccgccagca ggaggcccgc cgccagcggc aggcggatgc cgaaacgcat
     9661 gacgatgcgg gccgacagac ccagcgaaaa cgcggccatg atcaggttgg ccggaagaaa
     9721 acccagcccg acctgcagcg cgctgtagcc cagcaccagc tgcatgtaga gcgcggaaat
     9781 aaagaaccag gcaaacatgg cggccgccca caacacgccg acgatgttgg cgacggccac
     9841 attgcgcagg cgaaacaggc ccagaggcac cagcggcgtg gggacgcgcg attcgatgcg
     9901 caggaaaatc gccatcagca cagcggcagc ggccagcagc cccagcgtaa gcgccgaggt
     9961 ccagccggcc tcgttgccgt tgacgatggc atacaccgcc agcatcagcg ccgtggtcac
    10021 ggtgaccgca ccccatacat ccagccgcgc accggcggac tgcccgcgcc catcgggcag
    10081 cagcaccagg cacagggcaa acaccgccac accgatcggc aggttgacga gaaagatcca
    10141 gtgccagttg aaggcatccg tcagcacgcc accgagcaac acgccgatgc tgccgccgcc
    10201 ggcacagaca aaaccataca cacccatggc tttggcacgt tcggccggct cggtgaacag
    10261 agccatgatc agcgacaggg cgatggccga caccaccgca ccacccaggc cctgcacggc
    10321 acgcgccacc accagcagcc actgcgaatt ggccagcccg caggccagcg aggccagcgt
    10381 gaacagcacc aggccggcca gaaacagccg gcgctggccg tacagatcgc cgagccgccc
    10441 gcccagcagc agaaaaccgc caaaggtcag catgtaggcg ttgaccaccc agaccagcga
    10501 ggtttccgaa aaagccaggt cggtgcggat cgacggcagg gccacgttca cgatggtggt
    10561 gtcgagcacg atcatcagca caccgaggca aagcacagtg agtgcccacc agcgtttgcg
    10621 gctgtccatc ccggcattca tgcacacctc caggaaacgc cggacgccgg caatgcgggt
    10681 tggacggaaa gccgcaaggt cggcaggtcc atggttcggc tttcaaaaca aacgtgaaaa
    10741 acgtaggctg ccaccgaata ctcctttgcc tattgtgtac ggcgtacaca atagcatacc
    10801 aaagaccatc aacagcgtcc acatcacacc actcccgccc gcgttgatgc ccggcgctgc
    10861 gtcccgttgc tctgtttttg atagcttttt gcgcccgtcc ggaaagggct gcaggcactt
    10921 ttttcattca tcctgcaaca acggcctgcg ccggccccgg cacagctgtc ggcatgcact
    10981 gacagccagc ggggacagcg actgatgaag gaccgtgcca ccggcgccta aggtggttga
    11041 tgaatccagc cgcccgacgt acccatttat ccacttacgg agtccgcatg accaccaaca
    11101 cttccgacca gcaaaccctg tgggacctga tcaaggacat caaattcgga atgctgacgc
    11161 accgccacag cgacggcatg ctgcacagcc acccgctgac caccctgaac cagtccgccg
    11221 atgaaggctc gcgcctgtat ttcttcgttt cgcaccacag cgaaatcgcc cgtcgcctgc
    11281 agcaggacgg caacgtcaac gtgtcctatg ccgacccggg caaggacagc tacgtgtccc
    11341 tgtcgggcca gggcgcgatt gtcgaggatg gcgtcaaaaa ggcagccctc tggtcgccga
    11401 tggccaaggc ctggtttccg gaaggcccca ccgatccgga tctggcgctg ctggaagtca
    11461 gcatccgcca cgccgaatac tgggacgtga aggaaagcaa gatggtgcaa ttggccaaga
    11521 tggccaaagc cgcgctgacc ggcgagccgc ccaaaaacct gggcgagcac aaagaactga
    11581 gcctgtcctg aaacaccggt tgcacgacat gccacttctc tgctttaaca aggccacaga
    11641 ccctagcgca cccgacgacc tgcggatcaa ccccgcagcc gtgctctacg tcgaagcgtc
    11701 acggccggac cagatcggcc aggccatcat ccacctgctg ggccagggca ccagcgtcaa
    11761 cgcggtcacc cagtccaccg ccaccgtggt cagcgccatc ggcggcctgg tcagcgccac
    11821 gcgccactac ctggcgccgc cgcccgacga tcgggtcagc acggtgtatt tcgcaccagg
    11881 caatatcagc cacgtgcggc ccaacctgcc cgccgcgccg gacttctggt acgtgcgttt
    11941 cgtcgatggt tcggagctgc gcatcgtcga tccgctgccg gccgggctgt aaaagaaaaa
    12001 agccctgcag ccaggctgca gggcttgagc gcagcgccgt ggccggcggt gcatggtgat
    12061 gttggtggtg ggatcaggcc ttgtgcaaca gggcctgccg cgactcagtt gccccagtcg
    12121 gaatgctttt cgcaggtctt gttggtggac ttgcccttgg cggtttcagc gaacttggcc
    12181 aggctgccct tgaagtcctc cggcgtttcg ttccacatgc aggtgcaaaa cgcctgcgcc
    12241 ttggactggc ccttcaccgg gcttttttca ttgccgcctt cgtaattctt gtttgaatag
    12301 ctgatgcagg cggcatactg cttgtcgccc gccggagggg cgttgtaatc gtccacggcg
    12361 agcgcaacgg tgccggcgca cagcagcaca gccgacacca ggcatttgag actgtttttc
    12421 attgttttga tcccttatag gcaaaaagag ctttgccttc tcattgtaac gattgaaaca
    12481 atttttaaca ttctcgttac tgggctttcg gcatttcatc accgaataaa aaaaccccgg
    12541 gaccgaagtc acggggctcg ctgcacaggc agtcagcgca gcggcgggaa tcaggccggc
    12601 acgctgtctt tcaccgtgtc ggccatgtcg gtgtaatcct tgacctggtc gaagttgagg
    12661 tactggtaga cctcggcgct ggcggcggtc agcacaccca tgtcggccat gtattcggcc
    12721 ttggtcggga tgcggcccag cttggagcag atggcggcca gttccgcaga acccaggtac
    12781 acgttggaat tcttgcccag gcggttcggg aagttgcgcg tcgaggtcga gaacacggtc
    12841 gcgccctctt tcacctgcgc ctggttgccc atgcacaagc tgcagcccgg catttccatg
    12901 cgcgcaccgg ccgaacccag cacaccgtag tggccttctt cgctcagctg cttggcgtcc
    12961 atcttggtgg gcggcgccat ccagagcttg accggaatgt cgcgcttgtt ttccagcagc
    13021 ttggaggctg cgcggaaatg gccgatattg gtcatgcagc tgccgatgaa aacttcatcg
    13081 atcttgctgc cggccacttc actcagcgtc ttcacgtcgt ccggatcgtt cgggcaggcc
    13141 acgatgggtt cgtggatgtc ggccagatcg atctcgatca cggcggtgta ttccgcgtcc
    13201 gcgtcggcct tgagcagctg cgggtcggcc agccaggctt ccatggcggc gatgcggcgc
    13261 ttgatggtgc gctcgtccga gtaacccgag gcaatcatcc acttgagcat ggtgatgttg
    13321 ctggtgatgt actcgatgat gggttccttg ttcaggtgca ccgtgcagcc gccggcagaa
    13381 cgttcggccg aagcatcgct cagttcaaac gcctgctcga ccttcagatc gggcaaacct
    13441 tcgatttcca ggatgcggcc ggagaagatg tttttcttgc cctgcttggc gaccgtcagc
    13501 aggccggcct tgatcgcgta gagcggaatc gcattgacca ggtcgcgcag cgtgatgccg
    13561 ggctgcatct ggcccttgaa acgcaccagc accgactcgg gcatgtccag tggcatgacg
    13621 ccggtggcgg caccgaaagc caccaggccg gaacctgcag ggaagctgat gccgatgggg
    13681 aaacgcgtgt ggctgtcgcc gccggtgccg acggtgtcgg gcagcagcat gcggttgagc
    13741 cagctgtgga tgatgccgtc gcccgggcgc agcgggatgc cgccgcggtc gctcatgaac
    13801 tcgggcagtt catggtgcat cttcacatcg accggcttcg gataa
//
LOCUS       DAIDFG010000044        13609 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_44_length_13609_cov_17.541169, whole genome shotgun sequence.
ACCESSION   DAIDFG010000044 DAIDFG010000000
VERSION     DAIDFG010000044.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 13609)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13609)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13609
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44_length_13609_cov_17.541169"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2333 a         4137 c         4759 g         2380 t
ORIGIN      
        1 ggccatgctg cgtgctcctt ctgtatcagg cttttttcat gggcacgaca ttggcgggcc
       61 cgccgctgct gacccgtgcg ccggtcacga agtaggcgtg caggtcgtgg gcaaaacggt
      121 tgagcttgtg cagcacgccc ttgttgccgc gcaccagcgc gttgtagccg agcaccgccg
      181 gaatggcgac cgccagaccc agcgccgtca tgatcagcga ctcgccgatt gggccggcca
      241 ccttgtcgat ggttgcctgg ccagccgtgc ctatggccag cagcgcgtgg taaatgcccc
      301 agaccgtgcc gaacaggccg atgaagggcg cggtggagcc gaccgacgcc agaatggcca
      361 ggcccgactg caggcgcgag gtgaactcgt cgatggtgtt gcgcaggctg cgcgtgaccc
      421 agtcgctgac gtccagtgtg tcatgcaggt gcgctttggt gttgcggtgg tgggcggtgg
      481 cctcgcggcc ttcgatggcc aactggcgga aggggttggc cgggtcgttg cccagccggg
      541 ccaggccggc ggcgaagtct tcgctgtgcc agaagtcttc cgcgctgcgg gcaattttct
      601 tgtacttgag cacgtccagg gtcttggtga ggatgacgat ccaggacacc agcgacatgc
      661 cgatcagcag cagggccacc gacttggtga cgaagtcgcc ctgcgtccag acgttcatca
      721 ggccaaattg tgaattcatg agttactcca gaacaaaatt aatcggggct ttgagcagga
      781 actgggtgtc ttcattgacg cccgcccgtc ggaaaggggt gacctgcgag gccatcacgg
      841 catcgcgcgc ggcccggtcc aggcgctcga agccgctgga cttgaagact tcggcgcgtt
      901 tggcgtggcc ggaggtgctg tagtaaacgg caatgatgac cacgccggat tcgccgctgc
      961 gcttgctcag cgccgggtaa accgggcgcg gtggccggat gtattcggtt tccccctggg
     1021 tgatttccac cagccgcgcc ggggccggcg gcgccggagg cgcgggggcg ggcgccaccg
     1081 gcgccagcac cgcgggtgcc ggcggctggg gttcgaccac gccggtgggc gcattcggcg
     1141 ccggcacggg gtccagcacg gccacgggca tgggcggtgg cggcagcttg gccgttttcg
     1201 gcgcgggttt ggggggcggc gcgggcggcg gtggcgccac cttgggcggc tcgatttcga
     1261 tgatgcgcgt cagcagctcg gccggcacca cctgttccac ggcccggcgc agcaggccgt
     1321 tctgcagcgt ccacagcgcg gccacgtgga agatgaccac gctggcggca ataacgatgt
     1381 tgcggctgat cggcaatcgc acggggcttg tcggaagtgc aggggcgttg gagcccgaaa
     1441 tggcgggagc gttcatcgga agtctagtgg atgcgaaggg aggaacgcgt gggcgggaac
     1501 aaggcgggca gaaagcgggc gcgctagcgg cggaaaatcc accagagtgg gctgatcacc
     1561 aggatcaggc tgccggcgag gcttgcaaag agggtgagag aggattccat gggctgcctt
     1621 ccgttgggtg gtcggcgcgc ttgatgtggg ccgtggggca ggtggcgctg cattgcgcca
     1681 ccacgtcccg ggcacaccct tcacattgac cgcactgtgt ggcgacgccg agctcgaact
     1741 ggatctcgtc aaagcccagg cctgcgcggg cgtgacgggc gatttcgcgg tcggagattc
     1801 ggcggcagac acagacgatc atgggattgg cttgctggtg gttaaatcga tgtgtgaagt
     1861 ataaatgcga atcgatcgca tttgcaataa atccttgatg cccctgaaaa aatcagggca
     1921 tcggatcgaa gaacagctcg ggcgaggcct ggtccagggt ggcgtagccg cacagcgcca
     1981 tggcaatctc gagctcgtcg cgcagcagcc gcagcacatg cgccacgccg agcgcgccgg
     2041 cattggccag gccgtagacg cagggccgcc ccaccagcac ggcacgcgca cccagggcga
     2101 tggccttgag cacgtccgtg ccgcggcgta tgccgccatc gaccagcagc ggcaaatcac
     2161 ccgcgacggc ctgggcgatg cgcggcagta ccgccgcggt agccggcgtg gtgtccagcg
     2221 tgcgcccgcc gtggttggag acaatcagcg ccgccacctg cagcgcggcc gcctggcgtg
     2281 cgtcctgtgg atgcagcaca cccttgagca gcaccggcaa ccgtgtggtt tcctgcagcc
     2341 attgcacgtc cgcccaggtg ggcgcgtgcc cgggcaggct gtcgaacagg ctggcgccgg
     2401 ggtgggcggg gggcggcgcg agctgcacca gattcacggc ggaaatgccg gccggcaagc
     2461 ggaagcccgc acgccgctcg cggtcgcgcg cgccgctggt aggtgcatcc accgtcagga
     2521 ccagcgcttc gtaaccggcg ctttcggcgc gctggaccag ctcccgcgtg aagccccggt
     2581 cgtgctggag atagagctgg aaccacagcg ggccgcgctc ggggtaggcg ccgaaagccc
     2641 gggccacggt ttccagagag acgctggcct gggtgctcag caccagcccg gcgccctggg
     2701 aggccgccgc gtgcgcggtg gcgatttccc catcggcatg cgccatgcgc tggtaagcca
     2761 ccggcgccac cagcagcggg tgggccagcg tgcgccccag cagctcgaca catgtgtgcc
     2821 cgccggccag cgggcgcagc acacgcggca gcaggcgaag gttgtcccag gcgctgcggt
     2881 tggcgcgcag cgtcccttcg tccgcggcgc caccgctgaa ataggcccag gccgggtcgt
     2941 ccagccgcgt gcgtgcaaaa gcttcatggt cgtccagcga caccaggccg gccggcagcg
     3001 tctcgacggc gggaacgggc acgggcatcg gttgggccgg gttcatgtgt cggcccacat
     3061 gcgcagcagg ttgtggtagc tgccggtgag ctgcaccagg gccggcccct cgccctggcg
     3121 ccgcaactgc agcagcgcca tgtccatgtc gaacagcaac tggcgttgct ctgtgctgcg
     3181 caccatgctt tcaatccaga aaaagctggc cagccgcgtg ccacgggtca cggggctgac
     3241 ctggtggagt gtgctgccgg gataaagcac cagatcaccc gcggccagct tcacacgttg
     3301 ttcgccctgt acctgctgga tcagcagttc tccgccgtcg tagtcgcccg gatcggacag
     3361 gaaaacggtg caggacatat cgctgcgcac ccattgcgat gtcgctttgg agtgcagcac
     3421 ggcgccatcg atatgggggc cgtagaaatt ggcgtcgccg ctgtaacggt tgaacagcgg
     3481 gttgaagatt tttttcggca atgccgccga gaaaaacaga ggatcccggt tcaccgcggc
     3541 caggatcatg gtctggagct ctcgggcatt cccgctgtcc tgggacagct gctcgttctg
     3601 ttttttcgac accgcctgcc cccccgcgct gctgcgcccg tcgatccagg gcgcgtcgct
     3661 gccaagcagg cggcgggcgg tctggagttc atcttgggac aggatgtttt tcagatgcag
     3721 atgcatgatc gtcactcagt gcgggagggg gcggtggcag gatggcgcct agaacagggt
     3781 tttcagggtc aactggacag ccctcggggc gcccggcgcg tagaagccgc ggtacagcga
     3841 gtctgcgtag agcttgttgc tcaggttggt cacgttgagc ttgagtgatg ccttgtcgtt
     3901 aagggtgtac tcgaccatgg catcgacggt cgtgaagctg tcggccagca catggcgcgc
     3961 gccttccggg ttttgcttgc cacggtagtt caggccaccg ccaacgcgca gcttgggcag
     4021 gatgcggtag gtggtccaca ggctggcgct gtgccgcggc gtcagcgcgg ggcggtcgcc
     4081 ctgcacctgc gcaccggttc cggcggcgtt ccgtgccaca ttgctctggt caatcttcgc
     4141 ttccggaatc caggtgtggt tgtagaagat ctcccatttc ggcgtgatgc tgccggccag
     4201 gttgaattcc aggccggtgg catgacgctt gccggacagg atgttttgct gggcagcggt
     4261 gtccgggtcg gtattgcgct cgttgtactt ctcggtgcgg aacagggcga cgcccaggga
     4321 agcacgtttg tcaaaaagct cgaacttgcc accgaactcc aggttccggc ttttctccgg
     4381 tccggtcttt gcatccttgc tgttcgggtc cccgggggtg aactggtagg cgtccgccga
     4441 ggtgttgtaa gacgttccca gcgaagcata gtaggaggag atgtcatcgg gctggaacag
     4501 cgcacccacg cgcggactcc acagcccctc ggattgctca tggctgacac ccgcattggt
     4561 gcggtaggaa gccttgaagt tgtcatggcg caagccgccg accagcttga aggtcgatgt
     4621 cagcgacatg gtgtcctgca cgtacagtcc caggttgcgc gccttgaagg tgttgaaggg
     4681 tggcgctccg cgggtgtccg cgcgccagtc gccatcgttg ggcgtgccga cggtgctggt
     4741 caggttgctg gcggcgccgg caaaattatt ggcgcgtctg gcatcctcgt cgtagatgtc
     4801 caccccggcg atcacgtcgt gttttttacc catccactcg aagctgttgt tgtagtcgct
     4861 ctgcaactgc gtggtttcgc tgatgccgat gcgccccttg ggcgtgcggg aaatgacggt
     4921 gttcgggttc aggttgctga gggtcgtgcc ggcgccaaag cggatcacgc tggcccagag
     4981 gtcgcgttca taacggccgt ggcgcagccg tgtcttgagc tggctgttgt ggtcaaaccg
     5041 atgggtgtgg ctgagggtgg cgtaggtggc cgaggtgtcc tggtagtcgc tggccaggcc
     5101 gtagtagttt ttggccggca acgttggaac aatcatgccg tcctgcgtga accaggggtg
     5161 gttgtagttg gggcgaccct tgacttccag atggtagaga ccgacggaaa actcgtcgcg
     5221 cgtgcctatg ccccagcggt aagtcggggc aatgcctttt ttgctgacct tggcgccatg
     5281 gttgtcagcc tcgtgaacca tggcattgaa gcgcagcgcg ctgtcctcac cggtcttgag
     5341 gttgaagtcg ccggtcagcc ggtgctcatt gcctgtgccc aaggtgtagg tggcttcgtg
     5401 ctggtccatc aggaagggct ccttgttgac ctggttgacg atgccgccgg tggagccctt
     5461 tccgaacagc atggacgcag agcctttcag cacttcgacc cggtcattgt tgaaggtgtc
     5521 gcgttcatac agcgcgccgt cacggatgcc gtcggtgtag atgtcgcccg cctgacccag
     5581 cgaaaagccg cgcagccgca cgtcttcctc accggtttca ccggcctgaa aggtcacccc
     5641 cgcggtggta cgcagcaact cacggaaatc gtcgaggttg cggtcatcaa tcaggcgctc
     5701 ggtcatcacc gtgacggact gcgggatgtc gcgcagggcc tgtgtgccct tgccgatgtt
     5761 ggtctgggtc gcgcgcagcg tggtcttgcc ctgcgcctgt tctttcacgg tgatggtgga
     5821 gagcgtgtcg gcggcgggag tggtttgtga ccagccgctg accgaagcgg ccagcagcaa
     5881 tgcgcccagc ggcagcaagg cgttctcgga ggcggatttc ggggagaagg cggcgggtac
     5941 gtgcgcggca acaggggtca ggctgcgacg ggattttttg ttgcgcgcca ggcgtgcgtg
     6001 agggtttgcc aagatggcct ccaagcgggt gtccggcctt gtagcggcaa cggaccgggt
     6061 ttcaggaaat ggcttggctg tgctcgaaga cgtcctggcg gacacgagtt ggctggctgg
     6121 tcaggtcagc tgcaagtgta aattaaatga taatgattcg taataagttg tacgctgaaa
     6181 gccggttatt ttctgaaagt cgcgggccct gtggcgtaaa agccatggcc ggccggagac
     6241 ggccggaaat cagccgggtg gcgggccggg cttgcgcgcc cagcggacgg ggtggacccg
     6301 cgcggcctgc ccggacgccg gggcgtcact gccgcagccg ctgcagcccg aggcgcagcc
     6361 ggccgccggt tgttgcgcca gcgcctgcag gatgcgcgag gcctgcaggt gggcagagcg
     6421 ggccatcagc gcccgtgcgg cacgctgctt cagcccggcc ggcaggaaaa tccagaggca
     6481 ataggcggca cacaccatga ccagcgtgta gaccgccagg gcttccagca ggtgatgcat
     6541 caggcggtca gcgccagcac ggcgcggtag gtgacaaacg aggcggcata ggccagcgca
     6601 aagaggtagg cggccatcag cagcgggtag cgccaggagt tggtctcgcg cttgaccacg
     6661 gccagggtcg agatgcattg cggcgcaaac acataccagg ccagcagcga gtaggcagtg
     6721 gcggtggacc agctctgcgc aatcagcggg ctcagggctg cagcggcgtc gtcaccggcc
     6781 gccgacagcg aatacaccgt gccgagggca ccgaccgcga cttcgcgtgc ggccatgccg
     6841 ggcaccagcg caatcgagat ctgccagttg aaaccgatgg gggcaaacac cacctcgagc
     6901 gccttgccta tcatgccggc gaagctgtac tggatggccg gacctagcgc gccgtcgggc
     6961 gggccgggga aggtggacag aaaccacaac accaccatca gcgcaaaaat gatggtgccg
     7021 acgcggtgca tgaaaatctc cacccgctgc cacaggccca gcagcaggtt gcgcagattc
     7081 ggcacgcggt agggcggcag ttccagcatc agcggctggt agctgctttt ggcccaggtg
     7141 cgcttgaaca cccaggccac cgccatggcc gacacgatgc ccagcacata cagcgcaaac
     7201 agggtcaggc cctgcaggtt gaagaagccg acttcacggt ccgggatgaa ggcgcctatc
     7261 agcagcgcgt acaacggcag ccgggccgag caggtcatca gcggcgcgat catgatggtg
     7321 gccagtcggt cgcgccagtg cggaatggtg cgcgtggcca tgatgccggg aatcgcgcag
     7381 gcaaaactcg acagcagcgg aatgaaggcg cggcccgaca ggccgacctt gcccatgacg
     7441 ttgtcgagca aaaacgcggc gcgcggcagg tagcccgagt cttccagcag caggatgaag
     7501 aaaaacaaaa tcacgatctg cggcaggaag accagcacac cacccacgcc ggcgacgatg
     7561 ccatcgacca gcaggctttg cagcaggccg ggcgtcacat ggccctgcac aaacgagccg
     7621 acggcggcga cggcttcctt gatcaggtcc atcggcaaat tggcccaggc aaacaccgcc
     7681 tgaaaaatca ggaacagcac cgtggacagc agcagcaggc cagccaccgg gtgcatcacg
     7741 gccgcgtcga tgcggtggtt gaaacgcttg acctggccgg cgttgggtac ggcggcggcc
     7801 aggatggccc ggacctgcac ctgcagggcg gccatgtccg gtgcgccgga tgctcctgtt
     7861 ttgatagctg ctggcgcaga aggggattgg gttggaggcc taaaagcctc aatattttgc
     7921 gccgtaaagg ctttctgggc ggcctgcagc agggcctcac gcccactctc gccggtcttg
     7981 ccgcgccagg cggcgaacca ggggttgtgc accgcgatgg tttccaccac cggcatgccg
     8041 aggtcggccg acagccgggc gatgtcgatc tgcaggccac gcgcacgcgc cacgtccatc
     8101 atgttgagcg ccagcaccat gggccggccc agcgcgcgca actgcagcac caggcgcagg
     8161 ttcatgcgaa ggttggtggc atcgaccacc gcgatgatgc cgtcgggccg gttttcaccg
     8221 gcgcgcaggc ccatcaccac gtcatgcgtg acaacttcgt cggtggtcgc cgggttcagg
     8281 ctgtaggcgc ccggcaggtc catcacggcc agctcgcggc cgtcgggcag gcgcgcgcgg
     8341 ccttccttgc gttcgaccgt cacgccggcg tagttggcga ctttctggcg cgcgccggtc
     8401 agcaggttga aaagggcggt cttgccgcag ttggggttgc ccaccagggc gatgcgcagt
     8461 ggtcctgcta cggcactgga aactgcggcg ttcatgcctt cacctggatg cagctggcct
     8521 catggcggcg cagcgcaaac accgtgtcgc cgacctggac cgcgatcggt tcgcgaccgc
     8581 ccgggccgcg ccgcaacacg ctgatggttt cgccgggcat gaagccgagt tcgctcagcc
     8641 gttgcagcac ctgggattgc gcatcgccgc cttcggcttc ccgcaggctg atgatggtgg
     8701 ttatccggtt ggggggcaga tcgaccaggg acagggtatt ccccgccatg gcgggggcgg
     8761 aaaaggtgga gcttacggcg agattcatga gctgtttctg gatcagtctg gcagttggcg
     8821 agcggcaagg tgaagcttgc ttgcaacgcc gttctttatg agaatcatta ccatttgcat
     8881 cttatcgcaa tccagcaggg tcgaggtgtt ttcgcagggt ggcgagtgtg gttgtcctca
     8941 ggacaaatag gcttgtatcc cccgtcggca acgctcatgg gcggagcttg aagcaccgga
     9001 aaggccgggc tcgcagccag cggggccgcc aggtcagggt cagggcaatg cccatggccg
     9061 cagcgcagac cagcaccacc cacaacaggc tgccgaagcc ggcgccttcc gcggcaaggc
     9121 agacgggcag gcccaggccg atggccgtga aacccgttgc acgttgcgcc cgcaggccag
     9181 gcgccggacg ggcgtggtgc ccgaaaacac gctccacatg gcgctcctgg ctcagggcca
     9241 gcaaggcaaa tccccacagg gccagcgtga aggcggcgag tgtcagcagc agatcacgca
     9301 cggctgatgc ctttggcggt gcccagggtg ccttgcgttg cagtgggagc cggcggcgtg
     9361 tgctgcgaag gcgcggcctg gtgccccagg tgcctggccg tgccggccgc caagcctgcg
     9421 agggccagca gcagcaggtc catgcctgcc accgcccaca agccacggcc cagtgtgtgg
     9481 gcgagatggt ggctggtggt agcccagttc agcgccaccg ccagcagggc cagcccggcc
     9541 accgcccagg cctgttcgcg ccaggcggcg cggccgcgca gcgcggcatg gcccagcgtg
     9601 cccagccagg ccaggtaaaa ggcccagacc tcgatctcgc tgcggtccca gcctgccagc
     9661 gccgcaccgg ccggcaacag ccggttggca gcaaaaaaca ccatcgtggc gacgatgatg
     9721 ccggacacgc tggtgaccgt gagcgcctcc accacacgta cgccggccag gccttttctg
     9781 gcgtggctgg cgcgccgtcc ttccagccag aacaggctgc cggtggccat cagcacgcag
     9841 ccggccaggc ccgccaggaa atacagccag cgcaaggtcc agtgttcgaa ctggatgaaa
     9901 tgcaggccgc tgatgaaacg ttccacggtc atcaccggcg cggcctcgaa gctgctcagc
     9961 acggtgcccg ttgcagtatc gaagtagagc tggtcgagat tcatggtcac catgtcggca
    10021 taaccgcggc gcagttccac atagctgttg gcatcgcccg ggtgccagac acgcacgaag
    10081 ctggcccggc cgccttgcca ctcctgctcc gccttcgcca gcatcacgtc aagcgacgcc
    10141 agcggctggc ccggcgtatt cgccttggcc cggcggtatt ggccgaagcc ctcggcggca
    10201 aaagcctgcc gcggattttt agcctgcgca taagtggcgt ggtaggcact cggccagtag
    10261 atggtgaaca ggatcacgat gcccgacagg gcgatggcaa agtaaaacgg catggccagc
    10321 acgccggtga ggttgtgcag gtccaggctg gcgcgctgca ggcgcttttt ggggcggaac
    10381 aaaaagaagt ccgcaaaaat cttgcggtgg atgatgacgc cgctgatgat cagcagcagc
    10441 atggacattc cggccaggcc gaccagccag taaccgacat ctttccagcg gatgtgcagg
    10501 cggtagtgga aaggaaagat gaagccggtg cctgcgagcg tgtcggtgtg ttcgagcacc
    10561 gcgccggttt gtggatgcag gtagcgccgc tcggtgacct tggtggcttc gtcgcgccag
    10621 cgcagctcca gcgccggcac gcgttcggtg ggcaggcgta caaaccattg cgacgatccc
    10681 cgggccaggc gctgggcctg ttcggtcacc ggtccatcca gcttgaaagg ctgtgcggca
    10741 gcggacggcg cgagccgtgt gcccggcatc atccagcggt cgatctcgcg gtcgaacacg
    10801 ctgagcgtgc ccatccagaa aatcgcaaac agcaggctgc cgatcaccac cccggcccag
    10861 gtgtgtagcc aggccatcga tttgcggaaa gtgggttcca tcgcgcgcat ctttcttctt
    10921 tagcgaatca gttgaaacag gaccaaggcc agggccgtgc cacccgccag cacagcccag
    10981 agccacgcca ggctgcgcac gctgaaggcc cacagcagca ccagcaggta gagcacaaag
    11041 cccagcatcg cggcgctcac cacggcttcg ctgcgtgcca ggccgagcag cggcagtgcg
    11101 gcggccagca gcgccaccag agccgcggtg aaggcatacc cgcccggcag cgccgcgatg
    11161 aggcgcagca cgatgcgcac cctggggcgg gcagccgcgc ggcgcaaagg cggaggcacg
    11221 agagccgcga tccatccggc cacagacatc ggcttcaatt tctggatggg ttcaaaaaag
    11281 atagcggcat gcgcccgacc catcagggct ggaggcatat ttcccttgtt gttttgaaga
    11341 aaggggcgca cgccggaagc ctcagttcat cttgttcggt gttgccgcgg gtgaggcagg
    11401 cagggcggcc aggccttcgg gctgggtgat ggtgagcgag gtcacatagc tggcgcggtc
    11461 gtatttgtcg gtgccgcgtt cgccggcgct ggtgtcggca tgtttgactt cgagcacgta
    11521 gctgcctttc cacggcaggc tgagtttgag ccggccctgg tcgtcaccgc gcaacgcctt
    11581 gccccagccc gaaggtgttg ccacctcgac cttggccttg ggcagcggct ggcccttgta
    11641 gaacacctgc acttcgacgc cgtcggggcc gttgttgccg gtgggcacca ggtccagcgt
    11701 gagatggggg gcctgcttgt cgaaggtgtt gaccaggcgg gccgcgggca cgtagaggct
    11761 gcgcaccagc ttgtcgccat ccttgcgttc cgaaaccggg tagtgcgcat cttccgccac
    11821 cagggactcg cctttggccg cgctgcccga caggacaaaa cccctgggcg tcttgctcag
    11881 ggtcagcggc tgggccgcgg caacgccgat gcgctgcgcg acgggcgcaa tgaatttgtc
    11941 gaggtagccg ggtgaggtct cgcgcaggtt ttgaccaaac tcgccgaagt acagcgtggc
    12001 gccgttggcg tcctgttcca gccagacatg gtgtgcctgg gccgtggttg cggtggtggc
    12061 ggcgaagaga ggggcgatga ggccgaggcg tgggaaagac atggtgatgg cgtcctgcag
    12121 gttgaagggg ggagggtgtt tttcctgttt tttcatgagg atgttcagaa cttgatgcgc
    12181 atgctgacct gcagctcacg cggcgcgcca ggcaggatca ggttgtcgtt gctgccgtgg
    12241 ctggagacga tgtatttcct gtcggtgatg tttttcaggt tcacaccgaa ttcgtagcgg
    12301 ccggtccgat agatgccggc caggtcaccg gtcaggtaag ccggcagcac gacgaggttg
    12361 gtcagcgaag cgaaacgctc gcccatgtag ttgaggccac cgcccaggct gaagccgttg
    12421 cccaggtctt tcatggccca gaaggaggct gaatggcgcg gcgtcagcgc cggcaccttg
    12481 cccagcgagg gcaccgaaac caccggcagc tgcagcgagc tgacgcgtgc cacggactcc
    12541 accatttcgc cgtccaggta ggcgtaaccc gcgctcacgt cccagcggcc cggcaggcgg
    12601 ccgctggccg tgagttccag cccgttggtc cgctgcgtgc ccacgttgat ctgcaggctg
    12661 gggttggccg ggtcggtgtt cttgatgttg ctgcgctcca ggttgaacag ggcggccgtc
    12721 acgctgagcg cgccgtccag gaaaccgagc ttgctgccga tctcgacgtt gcgggtgatc
    12781 tccggttcgt tgcccacgtt gctgccggcc agggcaaagg tctcggccga gggctggaac
    12841 gaacggctgt acgagaggta gtgtgactgc gcgtcggtgg gctgccacac caggccggcg
    12901 cgcggactga aggcgcggtc ggtacgggcc agcggtgcga gcttgcgctc gtaagtggtg
    12961 gactgcttga actcgtcgta gcgaatgcct gccagcgcct tccagttttt cgtcaacgtg
    13021 acctggtcct gaaggtacac acccagcacg tcctgcgtgg tgttgttcgg tatggcgctg
    13081 tcggccgcga gattagccgc ggtgaatgcc ggcaccgaca ccaggcccgg attgaagatg
    13141 gacacgcgtg ccacctgacc tgcagagatc gattgggagc ggcgcttctg ctggcccatt
    13201 tccaggccga aaagccattc ctgcttgaag ccgcccagcg tgtttttgta ggtgaaatcg
    13261 gtctggttga accagcccga ctcgtcacgg aagacggcgc tgcgggtcag cccgacggcc
    13321 agcgccaccg ggtccgtggt gccgctgggc agggtgttgt agcggtccag ctcgtagctg
    13381 tagtagcgcg tggtgttacg cacgctgaat acgtcgttga agctgtggtt cagggtggcg
    13441 gtgtaggtgt cgaccaggct ggtgctggtg tcgtcgcgcc gggcattgcc cgagccgtaa
    13501 taggtgctgg cgggtacatc gacggggcgg ccgttgagcg ccgggatgcc gaagtccgtc
    13561 acgcgctggt cgcgcgcctg ggtgtattgc agcagcaggg aagtctgct
//
LOCUS       DAIDFG010000045        13583 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_45_length_13583_cov_18.979746, whole genome shotgun sequence.
ACCESSION   DAIDFG010000045 DAIDFG010000000
VERSION     DAIDFG010000045.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 13583)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13583)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13583
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45_length_13583_cov_18.979746"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2499 a         4497 c         4282 g         2305 t
ORIGIN      
        1 ccaacggcct gctgcgcgac ccgctgcaca tccaggtgac cccgcgcaac accacggtgc
       61 agcgcatcac gcagaccatc cacccggtcg gccgcggcaa gaaaaaagcg ctgctcacgc
      121 acatcatcaa tgagcacaac tggagccagg tgctggtgtt cacacgcacc aagttcggcg
      181 ccaacaatgt ggccgaacac ctgacaaaaa acggcatccc cgcgatggca ctgcacggca
      241 acaagagcca gaccgcccgc acccaggcac tgcaggactt caagagcggc agcatacgcg
      301 cgctggtggc caccgacatt gccgcccgcg gcatcgacat cgacgagctg ccacacgtcg
      361 tgaactatga aatccccaac gtcagcgaag actatgtgca ccgcatcggc cgcaccggcc
      421 gcgccggcaa cagcggcgag gccgtgagcc tggtctgcct ggacgaggaa ggcttcatgc
      481 aggacatcga gcgtttcacc aagcagaaca tcgaaaaagt catcgtgccc ggttttgaag
      541 ccgagcccgg cgaaaaagcc gagccgctgg ccatgggccg ccagaccatc tggggcggcc
      601 tgggcaaacc gccgagtcgt gacgtgatgc aggccgccgc caaggcagct agatccgaaa
      661 tgatgacgcg tatccgcgag aacaagcccc aggcaggccg aggcgccggc aatggcggcg
      721 gcggcaacgg tggtggcaat ggtggtggac gcggccgcag cggcggaggc ggcaatggtg
      781 gtggtcgcag ccagccccag ggcggccagc cgcgccagaa ttcgggctac gcacaaggcg
      841 gccagcctgc gcagcagcag gcacgcaacc cgaatgccca gcctccccgt ccacgcaaca
      901 cccaggagcg cgacgatgat gcccaacccg ccagccatgt ttcctcaggc ttcccttcct
      961 ccggccagcc cacaggcggc aaccgcggca actaccgcgg cggcggccgg ccagccggtg
     1021 gcggcggacg ctcgggtggc cctggtggcc ggagcaacgg gtctggtcgg tcgggaaatt
     1081 ctggctcgtt tactggccga taaaagctgg tcggcggtcc actgcgtggg ccgccgcaaa
     1141 cctgattccg tgcacccctc gctggtggtg catctgaccg attcgctcac tgagttccca
     1201 gccccgccgg tggacgatgt cttcatcgcg ctgggcacca ccatcaaggt ggcgggcagc
     1261 cagcaggcgt tccgggcggt cgatttcgac gctgtcgtgg ccctggcccg atcggcgcgt
     1321 gccgccggcg caacccgcct gggcatcgtc agcgccatgg gcgccgacac cgagtcccct
     1381 gttttttaca accgcgtcaa aggcgagatg gaagacgctg tcagccagct cggctacaac
     1441 agcgtggtct tcgcacgccc ctcggttctg gcgggcgacc gcgcggtcct ggcacaggcc
     1501 acccgccccg gcgaacagct cgccctggtc gccatgcgcc tgctcaagcc gctgatcccg
     1561 cgcaactacc gcgtggttgc agcggccgac gtcgccgcgg cgctggtgaa cgccgtcaag
     1621 gccggtcagc cggggcgccg cgtgttgctc tcgggtgagc tgcagttctc ctgaaaatta
     1681 gcggcctttt tggcctccag ccattgacag acgggcgcct tcagctactg attcaatagc
     1741 aatgggtgct ttgccggcat gcctttcagc cctgcagaaa cagcgtcacc agcgggtccg
     1801 gcccgacctg gcggctccag tggccgtgga tggccgcgtc aaacgcggcg cccggcggca
     1861 gcagatcggc cgaataaaaa tgctccccgg gcttgaacac gcgcgatgtg ccgtcctgca
     1921 ggccgatttc catctggccg ccgagaatga ccacccactg cggtgtcccc gtgcaatgaa
     1981 actggctgcg aaaccccacc gggctgtggc gcagctggta accggtggac ggcatcaagg
     2041 gcgacaacag cgcttgcggc gacccttcgg tcagcgccag cggctcctcg cggaatccgg
     2101 cacggccatc gggaccggtg tagagcgtgg tcttggtgaa aacagtcatg ggcaatccgt
     2161 gaaaagtaaa tgaagggtgc gttcatgcgc cggtggctcg ccgccaggca agcacagcgg
     2221 ccacgatatc ttcgggccgg ctggccaagt cgaagctttg tgcattttcc tccgggccgg
     2281 gcgcttccag cgtgctgagc tggctggcca gcagcgacag cggcatgtaa tggccggtcc
     2341 gcgcggccat gcgggccgcg agcaaggggt agtcgccctg caaatggaga aaacgggcgt
     2401 cgggcgcacc gcgccgcagg atgtcgcggt aggagcgctt gagcgccgag cacgacagca
     2461 ccaggccgcg cccggccgcc tgcgcctgct ggagccgacc ggccagcgtt tgcagccagc
     2521 cctcgcggtc ctcgtcgctc agcgccaccc cggcggccat gcgcgccacg ttgctggcgg
     2581 gatgcaggct gtcgccctcc aggaactcca ggcccagggc ggccgccagc tgggcgccca
     2641 cggtgctttt gccgcagccg ctgaccccca tgaccacaaa aatatcggaa cacatctgcg
     2701 tcacattctg ccttctcgat cgacgctggc gactaagccg accaacgcac cgtggcgtcg
     2761 gcgcgttggt gcaggtcggg cagcagctcc agccccaggc cgggtttgcc gttcaggctg
     2821 agcatgcctt gggtcaccgt gggcagctcg gtcacgagtt ccttgtacca gccggtgtag
     2881 aaggcgcgca cgctttcctg gatcagggca ttgggtgcgt gcagggacag gtggctggac
     2941 gccgcccaga ccaccggccc ggtgcagtcg tgcggcgcca ccggcagttg ccaggcgtcg
     3001 gccatggccg cgatcttgcg cgcctcggtc aggccgccgc accagctcag gtccagcatg
     3061 gcgacaccgg ccacgccggt ctgcaggtag tccttgaaac cccatttgta gctcagcgtt
     3121 tcgctcgcgc agatcagcgc gtcgcagtct ttcgaatact gtttgagcag gtccagcgaa
     3181 tccatgcgga tcgcgtcttc gtgccagaag gtgttgaagg gcttgagtgc gcgcgcgatc
     3241 ttctgcgcca tcggcagccg ccacaggctg tggaactcca ccatgatgtc catctgctcg
     3301 ccgaccgcag agcggatctt gcgaaacggc tccagcgcca ggaccaggtc cgcgttgctg
     3361 atgtattgcc catcgctttt ttcggccgcc ggatcgaagg gccagatctt catcgccgtc
     3421 acgccttcgg acaacaacga ttcggccagt tcgtcggcac ggtgcaaaaa cccttcgagg
     3481 tcttcatacg gcccggcatt ggcctgcttc gcgtcaccga tgccccagtt ggcgctggtc
     3541 tggttgacgg tgctgcgcac gtactggtaa ccggcgcagg tgttgtagat gcgtatgctg
     3601 tcgcggctct tgccgcccag cgccgtgtac agcggcatgt tggccgcctt gccgaaaatg
     3661 tcccacagcg cgatgtcgat ggccgagttg ccgcgcgtct ccacgccgga gccgcgccag
     3721 ccgaggtagc cggtgatgtc cttgttgcgc gcctcgatgg ccagcgggtc cttgccgagc
     3781 agcttggggc cggcccactc gtggatgtag gcttccacgg ccgcggcccc catgaaggtt
     3841 tcacccaggc cgaccagacc cccgtcggtg taaatgcgga cccagatgat gttgggaaac
     3901 tcgccaaggc gcagggtttc gactttggtg attttcaaag taggctccat aaaaaaggcc
     3961 gcaagagcaa acccctgcgg ccttgtgacg atacgaccaa tgggccgctt aaccgccgcg
     4021 cgccttcttc agcgcgtcca tcaccatctt gaccggctcg gcgccaatcg tcggaatgtt
     4081 tttgtcatag acgggtttga ccttgtcgaa catgcgtttc tgctcggctg atgacacttc
     4141 gttgacggtc atggttttct tgaggttggt cagggacttt tcattgagcg cacggttggc
     4201 aacgcgctgg accttctggc cctccatcgc cgcttcacgc agcacggcct gctcctgcgg
     4261 gttcagctgg tcccacagct tcttgctgta gagaataagg aagggcgtgt aggcgtggcg
     4321 cgtgacgctg aggtacttct gcacttcgtt gaacttggag gtttcaatgg tcacgaaggg
     4381 gttttcctgg ccgtcgatgg tcttggtttc cagcgcggtg aacacctcgc caaaagccat
     4441 gggcacggcg ttggtcccca gggttttgaa ggtgtcgatg aagatgttgt tctgcatcac
     4501 gcggaccttg acgccgtcaa aatcttccgc cctggcgacc ggccgcttgc tgttggtcag
     4561 gttgcggaaa ccgttctccc agtaggccag gttgatcagg ccggcttctt ccagcttctt
     4621 gttgaagtag gcgccagcgg ggccgtcgag caccgcgtcg gcttccttct cgttggcaaa
     4681 caggaagggc agatcgaaag caccgagctc cttgacgatg cccaccagcg gtgaactcga
     4741 ggtgcaaacc atctcctgcg tgccggcgcg cagcgcctgc gtggccggca ggtcgccgcc
     4801 tgcggcgccg ccccagaagg cattgatctt catcttgccg cctgtcttgg cggccagcac
     4861 ttcctgcatc ttcttgacgc cgacgccgac cggatggtct tcgttgacac cgttggtgaa
     4921 cttgatcgtg cggtcggcaa actgcgcgaa gctcgaagtg gccaccagca aggcggctgc
     4981 ggccagggcc agggttctgc gtttgaacat ggttgtctcc ttttatataa acgacagggg
     5041 ttgggaaagg gaagtcaccg catcagccac ttcagcggct cggtgacgat ggcgggaaaa
     5101 gcaatcagca gggccaacac ggacagttgc gccagcagaa agggcagcac acccttgaag
     5161 gcggtgtcca tgctgatttt tgcaacgccg cacaccacgt tgagcacggt acccaccggc
     5221 ggcgtgatca ggccgatggc attgttcatg atgaacatga tcccgaaata caccgggtcg
     5281 atgccggcct tgagcaccac gggcatcagc accggcgtga ggatcagcac cgtcggcgtg
     5341 aagtcgagcg cggtgccgac gaccacgacg agcaccatca tcaccaccat cagcagcatc
     5401 ttgttgtcca tgaaaggcgt catcatttcg gcaacttccg tcggaatgtt ggcgacggtg
     5461 atcagccagg cgctgaccat ggctgccgcc accaggaaca tcaccacagc cgtcgtcttg
     5521 ccggccgaca ggatgagacc gtagagtgcg gaccatttga gttcgcggta gatgaacatc
     5581 ccgaccacaa agctgtagac cgcggcgacc acggcggctt cggtcggcgt gaagacgccg
     5641 aacttcatgc cgccaataat gacaatgggc agggtcagcg caaaaccacc ctcggccgtg
     5701 atgtgcagcc ggccggccat ggacaacttg ggcgcgggtt gcacgtcttc cttgcgcgcc
     5761 agccaccacc aggtcaggcc gatggccatg cccatcagca ggccgggaac aatgcctgcg
     5821 agaaacagct tggtgatgga cacattgccg gccacgccga aaatgatcaa gccgatcgac
     5881 ggcggaatca ccggcgcaat gatgccgccc gaggcaatca ggccggaagc gcggcccacg
     5941 tcgtagccgg cagcccgcat catgggaatc agcagcgcgg ccagcgcggc ggtgtcggca
     6001 atcgccgaac ccgacagcga ggccatgatc accgcggcga tcaccgtgac gtaaccgagg
     6061 cccccgcggt aatgcccgac ccaggccatc gccaggttca caatgcggcg gctgatgccg
     6121 ccggcattca tgaattcacc ggccagcagg aaaaacggca cagccaacag cgggaagttg
     6181 tcggcaccgt cgacaaaccg ctgggccagg atctggctgt cgaacgtgac aaggccgctg
     6241 gtggcagcaa ggaaagccat cagcgtgacg ccgcagacca gcagcgcata ggcaatcggc
     6301 atgccaatgg ccatggcggc cagcaggctg agaataaaaa cgctgacggt catgccttgc
     6361 ttccctgcat ctgtttttcg acgtcggcca ggccttcggt ttcgaccacc tgcaccagct
     6421 cttcctcgct gatgcgcccc gtcaccagcc ggaccagcac gttgagattg atgatggcca
     6481 tcaccacgct gacgacatag ccgatgccgt aaatccagat catggaaatg ccgaccaccg
     6541 gcgaaacatt ggtgacctgc agttcgtgca tcttgaaagt gcccatgaag aagagcacat
     6601 tgcagaacag catcagtgct tcgctgagga agaggcagac ctttttgcca agcaccggca
     6661 ggtgtttgat cagcgtgtcc acgcccagat gcccgccttc gtgcatggcc agcatcgcac
     6721 cgatgtaggt cagccagata aagaagtacc tcgacatttc gtcggagacg gaaatgcccg
     6781 aattgaacgc gtaacgcagc accacattgc cgaacaccat gatgaccatg gccaccatgc
     6841 agacgaccac cagcagctcc agcaacttga agaaccagtg gatcgccgca tccatcgtgt
     6901 tttgaaaatt catctttcgc ctatggtctg gtgagatggc tcaggccgcc ttgagctgcc
     6961 gtgcgggact gctgaggatg ggcaggcggc ggcgcgaggc cagcacctct tcgatgtcct
     7021 ctttcgcgcc gtcgatcagc acgcggatcg cgcgctcggc ttcgtcgggc tggtgcgcga
     7081 tcaccgcgtc cagcacggca cggtgcagcg gcagggagtg ggtcgggccg tccttgcggc
     7141 gtgtggaaat ttcaaagctg gtgcgcagca acgcccccag cgccttgctc atctgcacca
     7201 gcatgcggtt gcggctggcg cgcagcaggc cctggtggaa acgcaggtcg tgcgtgacat
     7261 agtcgccgcc ctcttcgacg gcatgtttca tgccggcgta agcggcctcg acctcggcaa
     7321 tgtcctgcgc cgtggcgcgc tccgccgcca ggcgcatcgc ggcaggttcc accacgcggc
     7381 gcaggtcctg caggtcgcgc aaaaattcgg gggtcagccc cgccttggac tgccagatga
     7441 tgacgtcggg gtcgaaccag ttccagtggt cctccgacaa cacgcgggtg cccaccttgg
     7501 gcccggtggt catcaggccc ttggcaatca gcgacttcac ggcctcacgc accaccgtgc
     7561 ggctgacacc gagctcctgg cacaacaccg gctcgggagg gatcgacgcg cccacggcat
     7621 accggcccgc aacaatggct tcgccaaggt ggtcaaccgt attgccgtga acgtttttga
     7681 tcattgccgc ctgtttagtg gttattacta ggattcgcgg cgcaatggta tgacgataat
     7741 atgtttatca attaaggatt ttccctatga ccgaccccaa gaccccccgg aagaaacccg
     7801 aagagctacg cagccagcaa tggtttggcc gccaggaccg cgacggtttc gcctaccgca
     7861 gctgggtcaa gggcaagggt gtgccgcacg accagttcga cggccggccc gtcatcggca
     7921 tctgcaacac cttcagcgaa ctgacaccct gcaacagcca cttccgcacg attgccgagc
     7981 aggtcaagat cggtgtgtac gaggccggcg gtttcccgct ggagttcccg gtcatgtcct
     8041 tgggcgaaac cctgctgcgc ccgaccgcca tgctgtaccg caacctcgcc agcatggacg
     8101 tcgaggaaag cattcgcggc aacccgatcg acggcgtcgt gctgctgatg ggttgcgaca
     8161 agaccacgcc gtcgctggtc atgggtgcgg ccagcgtgga cctgcccacc gtgggcgtca
     8221 gcggcggccc catgctcagc ggcaagtggc gcgggcagga gctcggttcg ggcaccggcg
     8281 tctggagcat gagcgagcag gtgcgtgccg gcacgctcaa gctcgccgac ttttttgaag
     8341 ccgaaagctg catgcaccgc agccacggcc actgcatgac catgggcacg gcctccacca
     8401 tggccagcat ggtcgaggcg ctgggcatag gcctgccggg caacgcggcc tacccggccg
     8461 tcgatggccg gcgcaatgtg ctggcgcgca tggccggacg ccgtgcggtc gagatggtgc
     8521 atgaagacct gatactctcg aaaatcctca cacgtgaagc gtttgaaaac gccatcaaga
     8581 ccctggccgc gattggcgga tcgaccaacg cggtgatcca cctgattgcg atggccggcc
     8641 gcatcggcgt gaaactcaca gtggacgact tcgaccgcct ggccagcgag ctgccctgcc
     8701 tggtcaacct gcagccgtcc ggcaagttcc tgatggaaga tttctgctac gccggcggcc
     8761 tgccggtggt catgaaggaa atcgcgcagc acctgcacac gggcgccatc accgccaatg
     8821 gaaaaaccat aggcgagaac attgccgatg cacagaacta caacaccgag gtgatccgtc
     8881 cgctggccac gccgttcaag gacaaggccg gcattgccgt gctgcgcggc aacctgtcgc
     8941 cgcgcggcgc cgtcatcaag ccgagtgcgg cgacgcccgc actcatggtg cacaccggcc
     9001 gtgcggtagt gtttgaaaac atcgaggact tccacgcccg catcgacgac gaagacctcg
     9061 acatcgacga gacctgtgtc atggtgctga aaaactgcgg tcccaagggt taccccggca
     9121 tggccgaagt cggcaacatg ccgctgccgc ccaaggtgct gcgcaagggc atcaccgaca
     9181 tggtgcgcat cagcgacgcc cgcatgagcg gcaccgccta cggcacggtg gtgctgcaca
     9241 cggcgcccga agcggcggcc ggcggcccgc tggcgattgt gcagaacggc gacctgatcg
     9301 aactcaacgt gcccgagcgc aagctgcatc tgcatatcag cgacgaggag ctggcgcgcc
     9361 gcctggccga atggaaagca cccccgccgc cgctgtcctc gggttactgg aaactgtatg
     9421 ttgaccatgt gctgcaggcc gacgagggcg tggacctgga cttcctggtc ggcaaacgcg
     9481 gctccttcgt gccgcgcgac aaccactgat tcacaaggct tgacatgaaa ttactcatca
     9541 caggcggcgc cggttttgtg ggcgccaggc tggcccgcgc aattcttcaa aagggccagc
     9601 tcgatggcca gaccgttgac gagctgcaca ttgccgacat gtttgccccg ccggccgatc
     9661 tggcggccga cacgcgggtg cgggtgcaca ccggcccgct gctcaaccag tcggtcctgt
     9721 ttgccagcgg cttcgacgcc gtattccacc tggcctcggc cgtgtcgggc gagtgcgaac
     9781 tggacttcga cctgggcctg cgctccaacc tcgacagcac ccgccttctg ctcgacggcc
     9841 tgcgccaggc cggcaatgcc ccgcgcctgg tgttcgccag ttcggtggcg gtcttcggcc
     9901 cggatgtatc caacccgctg ccagacctcg tgagcgacca caccctgccg tcgccgcaga
     9961 cctcctatgg catgcacaag ctggtgtgtg aacacctgat aaccgactac acccgcaagg
    10021 gttttgtcga cggccggtcg gcgcgcctga tgaccgtcac cgtgcgcccg ggcaaaccca
    10081 acggcgcggc gtcctcgttt ttcagcggca tcatccgcga gcccctggcc ggcgtggaaa
    10141 ccacctgccc ggtggatgcg agcgtcacgc atcccgtgtc atcgccgtcg aacacggtgc
    10201 atggcctgat taccgtgttc gaggccagcc agcaagccat gggcgggcgg ctggcgctca
    10261 acctgccggg cctcaatgtc agcgtcggtg agatgctgca ggccctggaa gaggtcgccg
    10321 gccccgaggt ccgccggcgg gtgcgttttg aacgcgacga acgcatcgcc ggcatcgtcg
    10381 ccaactgggc gcgcggcgcc agcgccggtc gggcgcacgc cctcggcctg aagcccgacg
    10441 cttccttcaa ggccatcatt gaacagtaca tcgaggattg ccgcagcccc gcctacccgg
    10501 ccgatgccct gcagggcatg tcctgaacca tgcccaccgg caggcctgac gccgctccct
    10561 gcacgcagcc cgcatgaacg gccgacgatt gctggccgtg gactggggca cctcgtcgct
    10621 gcgcggcgcg ttggtggcgc ctgacggtca ggtgctggaa gaacgcgccc tggcacgcgg
    10681 cattctcagt gtggcggcag gcgaatttcc ggcggttttt gaaaacgcgt ttggcgactg
    10741 gatgacggcc ggcaccttgt gcctgatctc gggcatggcc ggcagccagc agggctggcg
    10801 ggaagcgccc tactgcgcct gtccggcggg tttcagcgac atcgccgcga ggcttgaatg
    10861 gctggagccc ggccgcatcg ccatcgtgcc cggcctcagc atcagcacgg acggtgtccc
    10921 cgatgtcatg cgcggcgagg aaacgcagat tgtcggtgcc ctgctgctgc tgggcatcac
    10981 cgacgcgcgc ctggtgctgc cgggcaccca cagcaaatgg gtcacggtcg ccgacggccg
    11041 catcacggat ttttcaacct ggatgaccgg cgagttttac gccctgctgc gccagcactc
    11101 gattctggcg cgcacgctgc ccgcggggga gccgccagcg gacaccgccg ccttcgagca
    11161 gggcgtggcc cgagcgctgg ccggccccgg cctgctgcac accgccttca gcacgcgcac
    11221 cctgtccctg ttcaagcacc tgccgcccga tgccctgccc agctacctgt cgggactgct
    11281 catcggcgaa gaactcaaaa gccagaagct gcagcgcggc gagtccgtgg tgctcatggg
    11341 cgcagaggca ctgaccgcgc gttatgaaca ggcgctggcg caactgggcg tgacggtccg
    11401 tcgcgtgggc gccagcgcca cctggcgcgg cctgcgcgcc attgcagaca ccttgcctcc
    11461 accccctcca ccatgaaccc atgaacgcct cccaaaactt tgccgctgcc ctgcagaccc
    11521 tgccgctggt cgccatcctg cgcggcctgc gccccgccga ggccgtcccg gtgggccagg
    11581 cgctgctgtc caccggctgg acgctgatcg aagtcccgct caattcaccg cagccgctgg
    11641 acagcattgc ggccctggcc gccgcctgtc cccaggcctt ggtcggcgcc ggcacggtgc
    11701 tgggtgtgaa cgatgtgcgc aatgtgcagg ccgccggtgg ccagctgatc gtctcgccca
    11761 acttcaaccc ggccgtcgtg cgcgaagccg tgcgcctggg cctggtctgc ctgcccggcg
    11821 tgatgacggc cagcgaggcc tttgccgcgc tcgaagccgg cgccaccggc ctgaaaattt
    11881 tcccggcgga aatgatcacg cccgccgtgc tgaaagcgct gcgcgcagtg ctgccggcca
    11941 agaccgtggt gctgccggtg ggcggcatca cgccggacaa catggaagct tatttcgcag
    12001 caggcgccag cggcttcggc atcgggtcgg cgctttacaa gcccggcatg gcggccgcag
    12061 aagtcgctga aaatgctaca aaattcatag ctgcttgcgc tggtaacata aaaacatgag
    12121 cactatttca tcctccaatt catcagtcat ctctttcggc ctcgcgggcc gcgtctgcat
    12181 ggtcacgggc ggcgcccagg gcatcggcga ggcgtgtata cggcgctttg cgcgcgaggg
    12241 ggcccaggtc gtcattgccg acattgacga cagccgcggc gcggccctgg ccgccgagct
    12301 gggcggcctg tatgtgcatt gcgacgtcgg cgacaaggcc caggtcgatg cgctggtggc
    12361 gcaaaccatg gccgcccacg gccgcatcga tgtgctggtc aacaacgccg gcattttcag
    12421 ggccgccgac tttctggagg tcaccgaagc cgacttcgac gccgtgctgc gcatcaacct
    12481 caagggctcg tttctggtgg gccaggccgt ggcgcgcgtg atggccggcg cgggcaaggg
    12541 cagcatcatc aacatgagtt cggtcaacgc cgtgctgacc attccgacca ttgccagcta
    12601 caacgtcagc aagggcggca tcaaccagct cacccgcgtg atggcactgg cgctggccga
    12661 taaaggcgtg cgtgtcaacg ccgtcgcgcc cggcaccatc gccaccgagc tggccgccaa
    12721 agccgtgctg accagccccg aggccactgc ccgcatcatg agccgcacgc ccatgaaacg
    12781 cctgggcgaa ccctccgaga ttgccgacac cgtggcctac ctggccagcg atgcggccag
    12841 ttacatcacc ggcgagatcg tggtggttga cggtggccgc atgaccctga attacaccgt
    12901 tccggtgtag ccggtccggc cggccacagg aatgcggctg attgccgctt cccgttgaca
    12961 tggatcatgg atacgcgtgg gcgaactccc aaaaattggc cagattcaat agactggccg
    13021 ttcaacaacc ggagttcacc atgatgtcaa ttcgcctgat ttccatcgcc gcagtcgctg
    13081 ccaccttggg tttggccggt tgcgcccaga cacgttacgg tcacatggcc tatcacagcg
    13141 gcgcaacacc ggcgtccgga caagccatgc ccatggccgg ccccggcggc cccatggcgc
    13201 gtatggacga ccacatgacc gccatgcgtt ccatgcatga caggatgtca cgcgccagaa
    13261 cgccggagga gcgcaacgcc ctgatggccg agcaccacaa actcatgcag gacggcatgg
    13321 gcatgatggg tggcatgggc cccggcggca tgggtggcag gggtggcatg atgggacctg
    13381 gtccgggccc catggctgct gatccggcgg cacgccagca atggatggaa aaacgcatgg
    13441 agatgatgca gtccatgatg cagatgatga tggaccgcct gcccccggca cccgccaaac
    13501 cctgaaagac accatgacct ccagcaccac gccctccgcc ggcgcagctt acggctttta
    13561 ctgcaagctg gacgacaaga gct
//
LOCUS       DAIDFG010000046        13522 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_46_length_13522_cov_18.881043, whole genome shotgun sequence.
ACCESSION   DAIDFG010000046 DAIDFG010000000
VERSION     DAIDFG010000046.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 13522)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13522)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13522
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46_length_13522_cov_18.881043"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2412 a         4516 c         4322 g         2272 t
ORIGIN      
        1 tccccctcca tcgcccagcg gggcgaggga ggggttaggg gtgggagtgg ggagtccgtc
       61 ctactgtcca aaaaggatcg taggcgcagc gtgaagcaaa taaaaaaaca aactcacacc
      121 ggccaaacca cccctttgtc attaaggatt gcatcaagcg caacatcatg cggctcgggc
      181 gccaggtcgg gcagccagcc gtggctgtaa cccagcccca cggtgaaggg tttgggctgc
      241 agcgttgcca atgtgcggtc gtagaaacca ccgccgtaac cgaggcgaaa gccacccggt
      301 ccgtaaccga cacaaggcac gaacagcagc gtcggcacga tgatctcggt gtccttgggt
      361 ttggggatgc cgtaagcgtc ttcctccatc gggcagccgg ggtaccaggc gtgaaaggtc
      421 agggttttgt ggaccttgtt caccaccggc aggccgatct tgcgcggcga ccggtttgct
      481 atgttttcag tagcagcttc cgcccgtccc tcaagggcca gaccttgatt tccttcggac
      541 tcctgactta aaatagcgtc ttcctgccag cggtacagcg cgggcagcgg atcaaactcc
      601 cccttgatcg gccagtaggc cccaatcact gcgtcctcgc ggcccaccag ccatatccgc
      661 atgacccgtt gcaacagatc ggcgcgctgt agccgatcgg gcaggctcag gcgttgttca
      721 atcaagtctc ttcgcgcagt taatttgtcc ataatgaccc gatgctatcc aaatttattc
      781 tgaaggccct gatttccggc ctgtcgtcct gtctgctgct tgcgcccgcc tgggcacaaa
      841 ccaaccatac cagcagcacc gcccgcggcg acgacgtgct gctggagatg aacaaggctt
      901 tcaagcgcgg cgataaagcc agactggccc agttgctgca gctggcccag ggccatgcgc
      961 tggagccctg ggccgcctac tgggaactgc gggcgcggct ggacgaagcc ggcgcgcgtg
     1021 aggtgcagga ttttctggcg cgttacgccg gcagctacca ggaagaccgg ctgcgcaacg
     1081 actggctgct gctgctgggc caacgccgcg actggggcgc ctttgccgcc gaatacccga
     1141 actaccgcat gggcgacgac aaggacgtgc gttgctacgc gttgctggtc gacggcctga
     1201 cgaacccggc cgcagttccg cgcgacgccg accaggtccg caagaactgg tacgcccagc
     1261 gcgaggccag cgacggctgc acatcggcgg cctcgcggct ggtcggcaac aagagtctga
     1321 ccacgcacga cgtgtggatc aaggccaggc tggcgatgga ggccaatcgc ccgcgcgccg
     1381 cccgcgatgc ggtcgagatc atcgcgcccg aagccctcgg gctggtgacc gaactcaaca
     1441 gcagcccgac gaaattcctc gccaccaagg taattgccat ccggcaggtg cgcaaggaaa
     1501 tcatcgtgct ggccctggtc aagctcgccg ccagcgaccc agatggtgcc gcccgcttcg
     1561 tcgaagacaa atggggactg caactcacgg ctgaggaacg caactgggtc tggggcgcca
     1621 tcggcaagca ggcggcgacc cggctcagca gcgacgcgct cgggtacttc gccaaggtca
     1681 gcaaggacac cgacctgagc gacgacatgc tgggctggaa ggtccgcgcc gccctgcgcg
     1741 ccggcagcac gccgcgctgg ccgctggtgc tgggcgccat caacgccatg agcgacgacg
     1801 cacgcaagga cccgacctgg gtctactgga aagcgcgcgc cctgctcagc aacgcgccgg
     1861 ccgccgtcgc cagcgcgccg ataccgccac agcgcgcaga agccctggcg ctgctgcaat
     1921 cgattgcctc ggtgcgcggg ttttacgaac agctggcgct cgaagagctg ggccagaaaa
     1981 tcaccgtccc accgcggccg gcccccctca cgccggagga aaaggaagcg gcacgcctgc
     2041 accccggact gaaccgcgcc gcgtatgcca tcgccctcgg cctgcgcagc gagggtgtgc
     2101 gcgaatggaa ctacagcacc aacctgcacc agcgcggcgg catgggtgag cgcgagctgc
     2161 tggctgccgc acagttcgcc tgcgaccgcg ccatctggga ccgctgcatc aacaccagcg
     2221 aacgcaccca gacggtgatg gacttcgagc agcgtttccc catgccgttt cgtgaagcgg
     2281 tggcccagcg cagccgcgac atcaacctgg acccggccta tgtgtatggc ctgatccgcc
     2341 aggaatcgcg tttcatcatg gatgcgcgct ccggcgtcgg cgcgtcgggc ctgatgcagg
     2401 tcatgcccgc caccgcgcgc gagaccgccc gttatatcgg cctgacgggt ttcaccgcgg
     2461 gccagatcaa cgaccgcgac accaacatcg tgatcggcac ggcttacctc aagcgcgcgc
     2521 tcgacgactt cggcggctcg atggcgctgg ccgccgcagc ctacaacgcc ggccccggcc
     2581 ggccgcgcag ctggcgcaac ggcccggtga tcgaagcggc catctgggcg gaaaacgtgc
     2641 cttttggcga gacgcgcgac tatgtgaaaa aagtgctgtc caacaccacc aactacgcag
     2701 cgctgctgac cggacaaccg cagtccctca aggccaggct gggcagcgtg gggccgcgtg
     2761 acgcttcggc accaccgccc gacgagaaga tgccctgaag cgcctgtccc agccgctcaa
     2821 ggggtgggac aggttttgta ggttttggcc tatgttccgg cccgtgctcc ccggctaaag
     2881 tgacatctcc tttccaggct ggagtgcaca gatgaacccg tggtggcaac aagtcggcaa
     2941 caccatcgtt gccgaattct cggacctgac cgacccgacc caggtgacac gcgccgtcct
     3001 gcgcctgctg gtggcggccc tgctgggcgg cctgctcggc tgggagcgcg agcaccaggg
     3061 caaggcagcg ggcgtgcgca cccacatgct ggtggccatg ggcgccgccc tgtttgtgct
     3121 ggtggcacaa caggccggca tggcggccga cgacctgagc cgggtcatcc agggcatcac
     3181 cgcgggcgtg ggttttctct gcgcgggcac catcctcaaa ggtgaccggc tcgatgacgt
     3241 gaaagggctg accacggcag ccgggctgtg gctgacggcg gcagtcggcg tggccgccgg
     3301 catgggacgc gagtccaccg ccgtgctcgg caccgtgctg gccctgggtg tgctggcggt
     3361 ggtgcccctg gtggtcaggt ccagcgaaaa gcctgcaccc ttgctggctg cggatgagcc
     3421 gccatccccc aagaggcgtt gaattgccgg gtcaggcttt caggcgctgc gagacgaact
     3481 cgccttcata gcccctggcc agcgacagtt tctcggcttc gctgagcacc ttgccggcaa
     3541 gctggaaaat gccctgctct tcgtccttca ggtgatgaaa aaccttgtgg tgcagctctt
     3601 tggcaaccgc cagccaggcc ggggaactga aatcggtctt ctccagtttt tctaccagct
     3661 cgtccatttc atggtgctcg gctatgccgt ggcgcgaagg ctcctgcgtg atgtcgtggg
     3721 caatcagcgg cacgtaaaaa aaccgctctt ccgccgccgc gtgggccgcc agttcggttt
     3781 tcagctcctt gaacagcagg tcgcgctcct tgctgtctcc cgaggtcttg atgaggttgt
     3841 ccgccagctc gcgttgggtg tcgtggctgg tgcgcaaggc ttcaaaaata ttcattcgtc
     3901 aattatgtaa agcccgccgt caagaaagtg taggccgaca agccgggatt tgttcaggcc
     3961 ggggcctaca acaacgcccg gttttgctgg cctgttttgc atcggccgac ctgaaggcgt
     4021 ctaatattgg ctatgaaaaa aatactgatt ctgggcggca ccggttttgt gggccgccat
     4081 gtctgcgaga aactcacgca actgcagtgg cgggtgaccg tgccgacccg gcatgaagca
     4141 aacgccaggg acatccagat gctgcccgcc ctggatgtgg tgccggccga tgtgcatgac
     4201 gaggcggcgc tgacccggct ggtggccgga catgatgcgg tggtcaacct gattgccatc
     4261 ctgcacggca cgcaggaggc cttccagcgg acccatgtgc agttgcccga aaaactggcg
     4321 cgcgcctgca ccgcaatcgg tgtgcggcgc ctggtccatg tcagtgcgct gggcgccgac
     4381 atgcgcaatc cggacgtggc gccgtccctg tacctgcgca gcaaggggca cggcgaggtg
     4441 gcgctgcacg cggcggccct cgagctcacg gtgttgcggc ccagcgtgat gttcggcgcc
     4501 gacgacaaat tcctcaacac cttcgcgcgg ctgcagaaaa tatttccggt cattccgctg
     4561 gcagccagcc acgccttgtt ccagccggtg tgggtcgagg acgtggccag cgccatcgtg
     4621 tattgcctgc aggacgccca cacccatgcc acggttgacc agaccttcga ggcctgcggt
     4681 ccgggcgtct tcacgctcag gcaactcgtc gaactggcag ggcggatgag cggcatcaac
     4741 gagggccgcg gccgcccggt gatcccgctg ccggcggcac tcggccgcct gcaggcgcgc
     4801 ctgatggaac tggcgcccgg cgagcccctg atgagccgcg acaacctgga ctccatgagt
     4861 ctgcccaaca ttgccagcgg caacctgccg gggctagaag ccctgggcat cacgccggca
     4921 gcgctggaag cgatagcgcc cacgtacctc ggcccgctca gcgacggctg gggcctgcgc
     4981 agccacctga cgctcaagcg caagaccgcg ggacggtttt aggtttagaa cttgttcctg
     5041 gtcccgggcc gcatcaatga ggcggtcttt tggcatcaat gaaaccgcgt ccacccggta
     5101 gcatggtgct ttttcatcct gggtgactcc atgctcaaac tctacatcgg caacaagaat
     5161 tactcgtcct ggtccatgcg cccctgggtg ctgctcaagc aggccggcat tgcgtttgag
     5221 gaagtcaagg tccgcttcga ctcgttcgag gccgtgtcca ccttcaagca ggccatccgt
     5281 accatcaccc ccgtcggcaa ggtgccggtg ctgctggacg gtgacctggc ggtctgggac
     5341 acgctggcaa tcgccgagta cctggccgag cagtacccgg acaaacagct ctggcccgcc
     5401 gacgtcaagg cgcgcgcacg cgcacgcagc atctgcgccg aaatgcacag cggctttggc
     5461 gccctgcgca gcgcctgccc catgaacatc gaggccgacc tgagcgacac cggccggctg
     5521 atctggcgcg acaaacccgc ggtgcgcgcc gatgtcgcgc gcatcgtggc catgtgggaa
     5581 gaattgctgg caagctacaa gggcccgctg ctgttcgggc aattcagcat tgccgacgcc
     5641 tacttcgcgc cggttgccat gcgcctgagc aggtatgccc tgccggtgcc gcaggaaatc
     5701 aatgcctaca tcgagcgcct gagcgcgttg cccggggtca aggcctgggt ggacgacgcc
     5761 ctcgcggaaa acgactttcg ggaattcgag gaaccgtacc ggttctcacg ctgatcatgg
     5821 caagaagcac catggtcacg atttacctgc gttacgtcct cgacacccac cggctcaagg
     5881 aattcgagca ttacggcaag ctgtggattc cactggtgga aaagtttggc ggaaaacacc
     5941 acggctactt catgcccggc gaaggcgcca gcaacattgc gttggcgctg ttcacgttcg
     6001 acagcctggc ttcctacgaa agttaccggc agcggtcttt tgaagacccc gattgccagg
     6061 ctgccttcag gtacgccgaa gaaacgggct gcattctgag ctacgagcgc agttttttcc
     6121 gccctgtctt ttcctgatcc gcatgcgggg tggcgccaac ccaaccgcta aactgccacc
     6181 atggaaattt tcatggtcgg tggtgcggtg cgcgatgcct tgctgggcct gccggtgcag
     6241 gaccatgact gggtggtggt cggcgcaacg cccgacgaac tgaccgccct gggctatctg
     6301 ccggtgggca aggacttccc ggtgttcctg catccgcaga cgcgcgagga gtatgcgctg
     6361 gcccgcaccg aacgcaacac ggcgcgcggc taccgcggct ttgccatcca cgccgcaccg
     6421 gacgtgacgc tggaggaaga ccttgcacgc cgcgacctta ctatcaattc aatagcagct
     6481 cccgcacatc ctcaaagaga tggagccgga tttgaggctg atcttgaagc gctcatcgat
     6541 ccctacggcg gacaggggga catccgggac aaggttctgc gccacgtcac cgacgccttt
     6601 cgcgaggacc cggtgcgcat cctgcggctg gcacgttttg ccgcgcgctt cgccgacttc
     6661 acggtggcgc ctgaaaccct ggcgctgatg cgcgagatgg tggcgcacgg cgaggtcgat
     6721 gccctggtgg ccgaacgcgt ctggcaggaa ctggcgcgcg gcctcatgga ggacacgccg
     6781 tcccgcatgt tcgcggtgct gcgcgactgc ggcgcactgc agcacctgct gcccgaggtc
     6841 gagaaactct ggggcgtgcc gcaacgcccc gagtaccacc ccgaagtgga caccggcgtg
     6901 cacctgatga tggtgctgga catggcagcg caactgcagg ccagcctggc cgtgcgtttc
     6961 gcctgcctgg gccacgacct cggcaagggc accacagcgg tcgaactgct gcccagacac
     7021 atcggccacg aggagcgcag cgcacgcctg ctcaagggcc tgtgtgcgcg gctgcgtgtg
     7081 cccaccgaat gccgggagct ggccgaagtg gtggcacgcg aacacggcaa catccaccgc
     7141 agcgccggat tcggtgcggc cgctctggtg cgcttgctgg agcgttgcga cgcatttcgc
     7201 aagccgcaac gttttgacga catcctgctg gcttgcgaat gtgatgcgcg cggccgcctg
     7261 gggctggcga acgctgccta tccgcagcgg cagcatctgg cgcaggcctt gcaggcggcg
     7321 caagcggtgg tgacggcgtc gatcgcagaa gcagcgcagg cggcaggcct gagcggcccc
     7381 aaaatcggcg acaagatcca tgccgcccgg gtgcaggccc tccagacgtg gatcgacggt
     7441 acagcggcgc ctgccgctac agccccgtcc gcctgacgag cacagggcgt cctgcagcgc
     7501 cgcctgtgcc agcagggaat ccggcctaca ggcgtcgcag aaaaattctg ctaaggtagg
     7561 aagcgatgca catgccctac gacacgctcc cccgtatcgc gcaggatacg gcgctttttc
     7621 tggactttga cggaaccctg gttgagctgg ccgcccggcc cgaagccgta gtcattccgc
     7681 ccaccctgac aagcacgctg gctgcgctgt atggacaact cggcggcgcg ctggcgctgg
     7741 tatcgggtcg ccgcctgagc gacctcgaca gttttctggc gccgctgcag ttgccggcag
     7801 cggtcgaaca tggcgggcag cgccgcaacg ccgacggtct cctgatcagt gcaccggcca
     7861 cggatctgcg cgatgcgctg caggcggccg aagggctgct gcagcgccat cccgctctcc
     7921 agctcgagcg gaaaaatctg gccctgagcc tgcactaccg gcacgccccc gacctggaag
     7981 acctgtgcct gcaggtgatg ggcgaggccg cggcacgcag cccggggctg cagcttatac
     8041 atggcaaatg tgtggtcgac ctgaagccca ctggggtcag caagggcacg gcgattgcgg
     8101 ccttcatgga cgaagcgccg tttgccggcc gtgttccggt gtttgccggc gacgacgtca
     8161 ccgacgagga cggcttcgaa caggtgctgc gcatgggcgg ccacgcgatc aaggtcggcc
     8221 ccggacccac gctggcgaaa catcgctgcg acaacgtggc gcaactggcc caatggctgc
     8281 tgtcggcacg ggcgcccgcg cgcgcgaacg gaccggaacg gcggcacgca tgagcacccc
     8341 agtccatccc tccagcttcg ctgcacccgc gccgccctca ctgtcgctgg gcgtgatcgg
     8401 caactgcgcc ttcagcgcgc tggtggatgc ccggggccgc attgtctggt gctgcctgcc
     8461 gcgtttcgac ggcgacccgg tgttcaacgc cctgctggac ccaagcgaga acggcagcgc
     8521 ctttgccatc gagatcgaga acttctcgca cagcaagcag tggtacgaac cgaacaccgc
     8581 cgtgctgcgc acgcagcttt tcgacaccag cggccaggca gtggagatca ccgacttcgc
     8641 gccgcgtttt taccggcgct cgcgcttttt caagcccatg acgctggtgc ggcgcgtgcg
     8701 gccaattcag ggcgcgccgc gcatccgggt ctcgctggac ctgcgttacg actggggccg
     8761 gagccggccg gtggtgaccc agggcagcaa ccacctgcgt tatgtcggcg atgcgctgac
     8821 actgaggctc aacaccgacg cgccgctggc gcacctgctg tcccggcaag cctttgtgct
     8881 gacacgcgaa cacaacttcc tgctgggcgc cgacgagtcg ctggccgacg gcattgccga
     8941 caccgcgcgc ctgttcgagg aagaaaccct ctcctactgg cgcaagtgga gccagcgcct
     9001 gcacattccg ctgcagtggc aggaggccgt gatacgcgcg gccatcacgc tcaagctctc
     9061 gctgtttgaa gacaccggcg cgattgtggc ggccatgacc accagcattc ccgaggcgcc
     9121 caacagcgga cgcaactggg actaccgctt ctgctggttg cgcgacgctt tttttgtggt
     9181 gcgcgcactc aacagcctgt ccgaagtcgg caccatggaa gactacctgc gctggctcag
     9241 caacgtggtg gtgcaggctg gcggcggcca tatccagccg ctgtacggca tcggcctgga
     9301 acgcgagttg cccgaggcgg tgcacggcca cctgagcggc taccgcggca tggggccggt
     9361 gcgcgtgggc aaccaggcgc aggagcattt ccagcacgac gtctacggca acgtggtgct
     9421 gggcgcggcc caggccttcc acgaccaccg gctgctgcac cgcgccggcg aggccgaatt
     9481 ttcgcggctg gaagcggttg gtgagcaggc cgtgcgcaac tacgaccagc ccgacgccgg
     9541 catgtgggag ctgcgcacgc gcgcccgggt gcacacctcc tcggccctga tgtgctgggc
     9601 ggcctgcgac cgtcttggca agatcgccgt caccctgaaa cagccggacc gcgccatcta
     9661 ctggcacggc catgccaaca ccatgcgcga gcgcatcctg cgcgaatcat ggagcgagga
     9721 acgccaggcc tttgccgaga gtttcggcgg gcgcgacctc gacgccagcg tgctcctgat
     9781 gatcgaggtc ggtttcatcg acccgaaaga cccgcgtttt gtcgcgaccg tggacgcgct
     9841 ggaaaaacac ctgtgcgacg gcccctacat gcggcgttac gaagcgcccg acgacttcgg
     9901 caaacccgag accgccttca acatctgcac cttctggcgc atcgatgcgc tggcgcgcat
     9961 tggccgcaag gaacaggcgc gcgagatctt cgagaccatg ctggctgcgc gcaaccacct
    10021 cggcctgctg tcggaagaca cgcatcccgt gaccggcgag atgtggggca acttcccgca
    10081 aacctactcc atggtcggcc tcatcaacgc cgccgtgcgc ctctctgcgc cctgggatag
    10141 ccaggtatga agggatggct cacccccgtc ggcggctcac tgcgtgtagc cccttccccc
    10201 cttgagtcat cggcacaccc ccgtcggaga ctcactgcgt gtagtccctt ccccccttgc
    10261 cgggggcgat acctacggcc cggcaaagcc ggttccgtgg tatccgcggc tggtgaaagt
    10321 tgtgccatat gagcagactt gtggtggtct ccaaccgcgt cgcggatccg cgcaagacgg
    10381 ccgccggcgg cctggcggtg gccctgggcg atgcgctgtc cgccaacggc ggtctgtggt
    10441 tcggctggag cgggacgatt gtcgaaggcg gcacgcccgg tgaaggcgag atgcacaccc
    10501 agcaggccgg caatgtgacg ctgaccacgg tggacctgtg ccgcgaagac cacgacagtt
    10561 attacctggg ttacaccaac ggcgtgctct ggccggtgtt tcattaccgg ctggatctgg
    10621 ccgacttcga tgccggcttc atcggtggct accgccgtgt caaccagatg ctggcgcaca
    10681 agctgatgcc gctgctgcgg cccgacgaca ccatctgggt gcacgactac cacctgatcc
    10741 cgctggcggc cgaactgcgc gcgatgggct gcaagaaccg catcggcttc ttcctgcaca
    10801 tcccgctgcc gccaccgctg atcctcgcgg ccgtgcccca acatgaatgg ctgatccgct
    10861 cgctgttttc ctatgacctg gtgggcttcc agagccaggc ggatgtggac cacttctcgc
    10921 gttatgtcgg ccaggaggcg ggcgccgaag ccctggacaa acaccagttc cgcgccttcg
    10981 ggcgcacggt gcacgcgcag gcctttccga ttggcatcga tgtcgatgaa tttgcggccc
    11041 tcacgcatgg caaggaagcg cgcgagatgt acgagcgcat gaaggaagaa tactcgcggc
    11101 gccgtctgtt gctgggcgtg gagcggctgg attactccaa gggcctgccg cagcgcctgc
    11161 gtgccttcag gcagttgctg aagatgtacc cggagaacct caacagcgca acgctgatcc
    11221 agatcgcctc acccagccgc gaggccctcg acacctacag cggcctgcgc caggaactgg
    11281 agagcctgtg cggcgccatc aatggcgact atggcgagct ggactggatg ccggtgcgct
    11341 acatccaccg caacgtggcg cgcaagcgcc tgcccggcct gtaccgcgtg gccagtgtgg
    11401 ccctggtcac gccgctgcgc gacggcatga acctggtcgc caaggagttc attgcggcgc
    11461 aggacccggc cgaccccggc gtgctggtgc tgtcacgttt tgcgggcgcg gccgaacagc
    11521 tcaaggaagc gctgctggtc aacccctacg acacccacgg cacggccgag agcatccagc
    11581 tggcactgca gatgccgctg cacgagcgcc agcggcggca ccaggcgctg ctcgagcgca
    11641 tccgtagcca ggatgtgcac tggtggcgcc agacctttct gcaaaccctg gccggcacca
    11701 cccccgaata gaacaagcgc cttctggcgc actgctggca gacaagccgg aaatgtgcaa
    11761 ccattcagtc atgaattgaa ctgacatggt caaacgcaat tacctcaacc gctccttggc
    11821 cgacggcata cgcaaggtgg gctttcgcaa atggtatgaa cacgagctga tgtccagcca
    11881 tgcccacatg ctgctggccc tgctgtgcac gattgccctg atggccacgc tggagctttt
    11941 tcagggcggc accctcaccg aaaagctggt ggacgtggtc ctgttcatca tcagcggcgc
    12001 ggtgggactg tgggccctgc gtcgctacct gtacctgctg ctgcatgccg aaaccgtggc
    12061 cgaccaggcc aattgcccga aatgcgccga atacggccgg ctcgatgttg tcgaggaaga
    12121 ccaccgcagc ggtcaggttc tcgtcaagtg ccgcaaatgc ggcgagccct ggacgctgat
    12181 tccctgaaac ccgggcggct tcttcagacc accagcgcca gcgccgcgta gtcgccgacc
    12241 cggtcctgca ggccggccgg caccagcaca aacccgtccg tcagcgccgg cagcctgccc
    12301 ctgatgtggg cctgcagacg cggcagcagg taatcgcggt ggtgccagaa cacgctgccg
    12361 ccgatgctga tgcgctgcag gtccagcgtc acgaccaggt tgtacagggc ccggcccatc
    12421 acccggcata gatcattaac aatggccaca gcgcccgtct ctcctgcgcg agcagctatc
    12481 aaaatagaag cagcatcggc atacccgagc gcgccaaaac gccgcggcat ggcattgccc
    12541 gccaccagcg cctcgacatc gccgatgttg ccgcagccgc acagcgcatc gttgttgtcg
    12601 ctgacgaaca tgtggccggc atggccggcg ttgccgtttt tcccgcgcag catctgcccg
    12661 tcgacacaca ggccgacacc gatgccggtg ctccaggtca cgtaagcgca atgcgccatg
    12721 ccctgcagcg cgccccagcg gcgctcagcc ttgagcgcag ccaggccgtc attttcaaca
    12781 cgcacctggg cgaatacctc gcgcagcggc gcttcgagca aggccgtggt ccagtcgttg
    12841 ggcagcccgc gcgccgcccc ggccaagccg ccgcagatgt tgggcgccgc gagttcgacc
    12901 tggccatcgt tcagcacaaa gggcccgcag gacgccacgc cgacggccgc caggtcctcg
    12961 cagcggatct gcgccgccac gctgctttcc ccgatcatgc gcagcacctg ccgcgccagc
    13021 gcatcgctga cgccctcctt ggcggtcggc tccacccgcc ggcccagaat gccgcgctga
    13081 tcgaccacgg caaccgacac cttggtgccg cctatgtcca cgcaggccac cggggcggcg
    13141 ccggcaggca ggcgaatgtc gggcccggcg aagggcggag cggtcggtag agggctgttc
    13201 atggcaggcg gttcaaatca tctggctgat gaggctggcc accaggctca ggacaatggt
    13261 ggtcgtcagc ggaatgaacc attcgcgccc aaacagtttg aagcgcaagt cgcccggcag
    13321 tttgccaaag cccagcttgt gcagcagggg cgtgaaccag ctgatgaaga tcagcgccag
    13381 gaaaatcacc agcatccagc gaatcatggg gctagtttag aggcaagcgc cttgcgccgc
    13441 gttgccgggt tgttgcgagc tgcagttgct tgcctgcaag ccgggtctcg ccccggcggg
    13501 cgaggcactt tgtctttgct tc
//
LOCUS       DAIDFG010000047        13426 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_47_length_13426_cov_17.154813, whole genome shotgun sequence.
ACCESSION   DAIDFG010000047 DAIDFG010000000
VERSION     DAIDFG010000047.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 13426)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13426)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13426
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47_length_13426_cov_17.154813"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2250 a         4158 c         4537 g         2481 t
ORIGIN      
        1 aaatcgggaa ggcgcggcgg ccggcgtcaa acaccggggc accgagtttt ttgccgttca
       61 ggtcggcgac ggtcttgatg ccggactttt tcagcgccat caccgaagcg ggcgtgttgt
      121 tgtagaccat catcaccgcg atcggcttat tgggggcatc cgggttgttg gcgtggaatt
      181 ccatcagcgc ggccaggtcg gcaaaaccca tgtcgtagct gcccgacgcc acacgtgtga
      241 cggtgccgcc cgagccgttg ccggcgtcga tcgtgacatc cagcttggcg tccttgaagt
      301 agcctttggc ggcaggggtc agaaacagtg ccgccggtcc ttcaaaacgc cagtccagct
      361 ggaacttgac gggtgtgttc tgggcgtagg catggctgcc caccgccgcg gctgcagtga
      421 cggccagggc tgcgaaggtc ttgatgaaag cgcgttttgt catgggtgct ccggggaagg
      481 ttggggatgt atcacgtcaa gcaagaaaaa tgccgggtcg gtggctggag gcaccctgtg
      541 cctctttgat tgtgggtcgg gtgcgcggcg cagccattgg gatatcccct gaaccggcgc
      601 accgggcaga aaagggatgg ttgacggcgc caggcgttca tggcgagggc gccagcagtt
      661 cacgcagcgt gcgcgcaatc accttggtcg cgccgcgcag atcgtcgaga accaggtgtt
      721 cgtcagcgcg tttggcattg gactcgagca ccgtgcgtgg cccggcgccg taaatcacgc
      781 ccggtatgcc ggcctcggcg tacaggcgca catcggtata cagcggcgtg cccatcgccg
      841 ggatgggctc accgaagacc gcctggccat gcttctggat cgcatcgacc agcggctggt
      901 tgcccggcag cggcttgagc gcattggcca gcagcaggcg cttgatttcg acgcggacac
      961 cttcgcactc cttggcggca tcattgatca ccttgcggat atcccgttcc acttcaaacg
     1021 gtttttcctc gggaatcatg cgccggtcca gcttgaacat caccttgccc ggcacgacgt
     1081 tggtgttggt gccaccttcg atgcggccca cattcaggta gggatggttg atgccctcga
     1141 ccttcgagct gaccttctgg tacagcgtgt tctgcgcgta cagcgcattg aggatgtgca
     1201 cggcggcctg cagtgcatcc acgccggtgt cgggaatggc ggcatgggcc atcttgccgt
     1261 gcaccgtgac ttccatctgc aggcagccgt tgtgggcggt caccacttca tggctgaaac
     1321 ccgcggcgat cagcaggtcc ggccgggtga gcttgttctt gagcagccag ccggggccca
     1381 tctccccgcc gaactcctcg tcgtaggtga aatgcagttc cacgctgccg tgcagcggga
     1441 catccagcgc ctccagcgcc cgcacggcaa aggtgtaagt ggcgaaatcg gacttgctga
     1501 cggccgaggc ccggccgtag atcctgccgt ccacgacctt gccgccataa ggttcctggg
     1561 tccagccttc accgggcggc accacgtcgc cgtgggcgtt cagcgcgatg gtcctgcctt
     1621 cgccataacg gcgacgcacg atcaggttgg tcagggactc gagcccgtag gccttgacct
     1681 ccaccggggc gatcttgtgt ttttccgctt cgaaaccgaa agtgtggaga agttcggccg
     1741 tgcgttcggc atgcggggca ttgttgccgg gcggcgtggg tgtgggcacg cggatcagtt
     1801 cctgcagaaa acgcacttcc tcgtcgaagt gggcgtccac ccaggcatcc agtttttcgt
     1861 catcagtcat ggctgggtcc ggcaaattgg ttcaacaggt gctggaaagc ccggacggcc
     1921 aggtcgatgt cgtcgctggt cgtgctttcc agcgggttgt ggctgatgcc ggaattctgg
     1981 ccgcgcacaa acagcatggc ctgcggcatc acctcgtgca gcttcatcgc gtcgtggccc
     2041 gcgccgctgg gcatgcggta aagcggaacg cccaggctgt ccaccgcttt ttcccagcgc
     2101 tgttgccagt ccggggcact cggcgctgcc gctgcgcgca tggtctgttc ggtggtgtgg
     2161 tggacaccgc ggcgttcgca aatggttttc agttcggcca gcacatcggc caccatcgcg
     2221 tcgcgctgtg catcgttggg cgcgcgcaag tccagcgtga actggcaacg gccgggcacc
     2281 acgttggtcg agccgccggg caccgtccag atgccgatgg tgccgaccga gtcgccgtcg
     2341 cgtgccgcgc gctgctccac atacagcgcg agttccgcca cggccagggc cgcgtcgcgc
     2401 cggcggttca tgggggtggt gcccgcatgg ctggccatgc cggtgacctc gcaggtgtag
     2461 cgcacaccgc cgttgatgga ggtcacgatg cccagcggga tgtcgagttc gttgagcacc
     2521 ggtccctgct cgatgtgcac ctcgacaaaa cccaggtaat cggccggctt gcgctgcagc
     2581 gcgcggatgg cctccagcgt ggccggcagg ccggcgtgtt gcatggcctc gcgcatgctg
     2641 atgccgtcgg cgtctgtctg gtccagccag gccggattga actggccgat caatgcgccg
     2701 gagccgagaa aagtggcctt gtagcgctgg ccttcttctt cggcaaaagc aatcacttca
     2761 aagtcgaagg gcaggcgctt gcccgcgcga gacagctcgg ccacgcaggc cacgggtaca
     2821 aaaatcccga gccggccatc gtacttgccg ccgttgcgca cggtgtcgta atgcgagccg
     2881 gtcatgagtg tcttcactcc ctctcccgct gggagagggt tggggtgagg gcgcgccttg
     2941 tagcggccca ccacgttgcc gaccgcgtcc acgctgactt catcaaagcc gctgtcccgc
     3001 atggtctgtt caatcagccg ggcggccgcg cggtgcgcgt cagtcaggta ggtcaccgtg
     3061 agctggcctt tttccaggta gcccgggtcg ctgaaaacgc cgagtttctc ctgccagtcc
     3121 cagacctggt tgcccagggt cggctccgca ccgaacttgt cgttgagccg caactcggcg
     3181 atgcggtgga tgttgcgcag ggcctcggcg agctcgaagt cggggtggtt ggccaggcgc
     3241 tgttcaaagg tggcgatgat ctcctgccgg ctcaagccgg tgccgcgcgg gccgcgcacg
     3301 gcgaggatga agggaaaacc gaaccgtgtg ttgtaggcgg tgttgagtgc ctggatgcgc
     3361 gtgaactcgt cgggcgtgca gtccgtcagg cctgccttgc cctgttcgtt cgtcgactcg
     3421 gcggtcagtg tcttgctgat catggccttg ccggccagtt cggggtgggc gcgaatcagc
     3481 gtcagctggg cctcgcggcc ggcttcgcga acgatgctgg ccgtcacgtg tttgaggtgg
     3541 gccagcgacc ggaaagggcg gtgtgccagc gcggcatcgg tgatccacgg ggaatgttcg
     3601 tagaggccgg ccagccaggc cgcggcttgc ggggccgggg cggtgttcag ttgttcaagg
     3661 gtcagggaca tggtcattct ttgaaggggt ggacttgctg ccagtggcgt gcgatgtcga
     3721 tgcggcggca gatccagaca ttctcgtgct gctggacatg gtcgaggaat ttctgcagcg
     3781 ccacgatgcg gccgggccgg cccagcaggc ggcagtgcat gccgatgctc agcatcttgg
     3841 gcgcgttcag gccgttcggg tcgccttcgg catacagcgc gtcgaagctg tcgcgcaggt
     3901 aggtgaaaaa atcatcgccc tgcgagaagc cctggggcag cgcaaaacgc atgtcgttgg
     3961 tgtccagcgt gtagggcacc acgagccgcg gcaccacggt gccgtcggtg cgcgccactt
     4021 tcatccacag cggcaggtcg tcgccgtagt agtcgctgtc gtaggccagc tggccgtcgt
     4081 cggcgaccag ccgccgggtg cgcgggctgt cgcggccggt gtaccagccc agcggccgct
     4141 ggccggtcat ctgcgtgatg atgtccaggc ccaggcgcat gtgttcgcgc tcgaccactt
     4201 cgtccacgcc ctggtagttg atccagcgca ggccgtggca ggcgatttcg tggcccagct
     4261 cgacgaaggc ggcggtcagc tcggggtggc gttgcagggc catgctgacg ccgaacacgg
     4321 tcagcggcag ccggcgcttt tcaaattcac gcaggagacg ccagacgccg gcgcgcgatc
     4381 cgtactcgta aatgccttcc atgctcaggt ggcgctccgg gtagctgggc gggttggcca
     4441 tctcggagag aaactgttcg gagccggcgt cgccatgcaa aacggcgttt tcaccgcctt
     4501 cctcgtagtt cagaacgaat tgcacggcaa tgcgggcctg acccggccac tgcgcatgcg
     4561 gcggcgtgcg gccatagcca accaggtctc tgggataggg cagggtggtg tcgtaggtgg
     4621 tcatgcgcgg gttcaggaca gggccatgga aatatcattg gccggcagcg ggcggtcaaa
     4681 gctgaggttt tcctcgacgt gctgcaggtg ctcgtccatc aggcgcacgg cgcgctcttc
     4741 gtccctggcg gccagggctc tcacgatttc ggcgtgttcg tcgtgcgagt gttcggcggc
     4801 gctggcgctc tggtacatca acgtgatcag cgagcagcgc gaaatcaggt cgcccagcat
     4861 ctgcgccagc acctcgttgc ccatcagctc ggccatgcgg acatgaaaat cacccagcag
     4921 ctcggtgcgc cccggcgcgt cctgccggtc cacggccatt ttttcctgcg ccacatgttc
     4981 cttcagggcc ttgatcctgg ccggtgtgac gctgcgcaca aatgcccggg tcatctcggc
     5041 ttccagcatg cggcgcaccg caaacacctg gcgcgcctcg gtgaccgaag gcgcagcgac
     5101 aaaggcgccg cgcgcgggct cgagccggat cagctggttt tgcgagagct ggaacagggc
     5161 ctggcgcacc agcgtgcggg aaacgccgaa gtggtcggcc agtttctgct cggccagctt
     5221 ggtgcccgga tgcagccggt gttccacaat ggccttggtc agggcttcaa caatggcgct
     5281 ggtggtggac gattccatgg cgcaaatgat agtcggaaag cccaaaagtg tatacacttt
     5341 cagtagacca tccggcgaac agattcaccg caaacaagga gttgacatgg gcctgagcac
     5401 acatgtgctg gacaccatgc acggcacgcc agcggccggc atgttcgtcg agctgtacac
     5461 caccgacggc gatcggacca cgctggtgaa agcctttcat ctgaacagcg acggccgcaa
     5521 ccccgacggg ctgctctatg acaacagcag cctgcggcgc ggcacctacc ggctggtgtt
     5581 cgacgtggcg gggtattttc gtgcccgcgg cgtgacgctg ccggagccga atttcctgaa
     5641 ccgggtgagc ctggacttcg gcgtggccga taccagccag cattaccacg tgccgctgct
     5701 ggtcagcccc tggagttatt ccacctatcg cggatcgtga acctgaggta gggcaccctg
     5761 cacagtgaac gcatgcgggc tctatctcta tttcaggcag gggccgcagg cgcagcggcc
     5821 ccctcggggg gcagggcgct acacgaagtg agcaaccgtg ggggcctaag tctgcccttc
     5881 ggtcagtgta tcgagctgga atttgccgct tttgtgccag agccaggtgt cggtgtagac
     5941 aaccttgcca atgcgcaccg gcgccgggaa aggcgacgcg gccttgatca tgcgttcgat
     6001 ctcggcaatc acttccggcg catggcgcgg tgcgcgcatc cagtcgaggc gggtcacgat
     6061 gccgacctgg ttgatttcca cgttcagcac accgatggcg tacagcagcg gcggcatttt
     6121 gcccttgtag atgcggtcgg cgtgcaaccc gtaaaggtgg ctggcgccgc tttggcggta
     6181 ggcgcgggcc gtcggtgcgg ccgattggcg cgccgagccg gccgggccgg agatcgggcc
     6241 tatcgtggtg ggcggtgccg caggctcggg tgccgctgcc ggcgggggag cggacttgca
     6301 ggcggaaaca agggcgccgg ctgcagtcga aagcagggcc gcaagccaga agcggcggtg
     6361 tgaggattca ggcatacgta tccttgtgta gttgccagac ggtggcggct gctattgtgc
     6421 agtctgcccg ggctcaggtt atgtaacaga aaccggctgc cccgaggctg aattatgcgt
     6481 tctgcgggcc ccgacagggg gccgtgtatc agagggtttc taccgtgagt cagctcaagg
     6541 atttgttttt ggcccggctg gcgctggagc cggcggtgct ggtcgaggtg accgccaccg
     6601 aaggctcggt gccgcgcgag gccggcacct ggatggcggt ttttgccgga acggcggttg
     6661 gcaccatagg cggcggccat ctggaatatg aagcgatggc gagagcgcgc gcacgtctgc
     6721 aggggttgcc gggtgaggcg cttggacgtt ttgtgctcgg tccgtcgctg gggcaatgct
     6781 gcggcggcgt ggttcatttg cgctttgaac ccgtcgggcc tgacgacgtg tcgggcctgc
     6841 gccgccggct ggatacaaat ttacagcctg ttgcgctttt tgggggcggg catgtgggtt
     6901 ttgcgctgat tcaggtgctc tcgcgcctgc cgtttgcgct gagctggatc gacagccgcg
     6961 acggcatttt cccggaggcg gtgccggacg acgtggtgtg cgagcattcc gacccggtgc
     7021 aacgcgcggt gactggcctg gcgccgaact cgcgggtgct gatcatgagt ttcagccacg
     7081 ccgaagacct ggacatcgtg gccgaatgcc tcaggcgcct gcgcgagcgg ggcgacctgc
     7141 cctacatcgg cctgatcggc agcaagacca aatgggcaac cttccggcac cggctggagg
     7201 cacgtggttt cagcgcggaa gaactggcgc acgtgacctg cccgatcggc ctgccaggca
     7261 tccccggcaa ggagcccgag gtgattgcgg tgtccgtcgc ggcacagttg ttgcatgccg
     7321 gcagggcata gatcgcatac acttttgcga tctggtcgca gcggagcact tcttgttgtt
     7381 cgtggccttt gtctgcttac agcgcccgca gtggtgagca gcttgcacaa ggcagcgccg
     7441 cgtcgctgca taggttgaga catggaaagt taccttctcg actgggccaa cctgctgctg
     7501 cgctgggtcc acgtcatcac cgcgatcgcc tgggtcggat cctcgtttta ttttgttttt
     7561 ctcgattcca gcctgacgcc gccggtcgat gaagacttga agaaacaggg cgtcagcggc
     7621 gaactctggg ccgtgcacgg cggcggtttc tatcacccgg tcaagtttgc agtcaagccg
     7681 cccaccttgc ccgcccacct gcactggttt tactgggaaa gttacagcac ctggctcacc
     7741 ggttttgcgc tgttcaccgt ctcctacctc tggaacgccg gcacctacct ggtcgacaaa
     7801 tcgctgatgg actggtcgcc cggcgccgcc atcggcgtgg cactttcttt ctttgtggtg
     7861 ttctggatgc tttacgacgg catctgccag ctgttcggca aacgcaaaaa cggcgatgcg
     7921 attgtgggcg cgctggtgct ggggctggtc tgtgtggcgt cctggctggc ctgtcactgg
     7981 tttgcggggc gcgccgcgtt tctgctggtg ggcgccatga tcgccaccgc gatgagtgcc
     8041 aacgtgtttt tctggatcat ccccgggcag cgcaaggtcg tggccagcat caaggccggc
     8101 gaggcggtgg acccggtgca cggccagcgc ggcaaacagc gcagcgtgca caacacctat
     8161 ttcaccttgc cggtgctgtt tgcgatgctg agcaaccact acagcttcac ctggggccac
     8221 ccgcagaact ggctggtgct gatggtcatg atgctggccg gcgcgctgat ccgccagttt
     8281 tttgtcatgc gccatggcta caagctgggc cgcaacggta acccgctgcc ctatgcgctg
     8341 gtcgggctgg tcctgatcct cggcgccatc gtctggatga agccgccgcc ccagcccgca
     8401 ggtgccgggc ttgctactga ttcaatagct ggtggcgtag gagtggcggg ggctgtaggc
     8461 caaaatgact atataaatct gcaaaaagtg ctggagcagc gctgctacct gtgccacggc
     8521 gcgcaggtgc agatgaaaaa catccgcctc gactcgccgg cgctgctcag gcagcatgcg
     8581 caggcggtgt accagcaggt ggtggtcagc aaaaccatgc cgatgaacaa ctccaccggc
     8641 atcaccgagg cggaacgggc cctggtccgg caatggttcg aagccggcgc gcctgtaccc
     8701 tgagaccgtc ctgagcacgc cttttaccag cgggtgacca ggcccgcggc ccgcgtggac
     8761 gatttctcga agtgtcccgc caggtagtcc acaaaggcgc gcacacgtgc cgacacctga
     8821 tggcgctgcg gccagaccgc atgaatgtcg gccagcgggg tctggtagtt ctccagcacc
     8881 tggcgcagcc ggccgctgcg caggtaacgc gcgacgtccc attcggcgcg catcacgatg
     8941 ccatggccgg ccagcgccca gttgacggca atctcaccgt cgctggtgct caggttgccg
     9001 cgcaccttca cggtttccgt gcgtttgccc gacgtcaggc gccagatgcc gtaggcctcg
     9061 tcgccctggc ggatgccgat gcagttgtgc tgcgtcaggt cgtgcggcac tttggggggg
     9121 ccgtgtctgg ccaggtaggc gggggccgca cacagcagac gccggttcgg ggccagcttg
     9181 cgggcgatca cacgcgcctc gggcggctcg ccgaagcgca cgcacacatc aaaggcatcc
     9241 tcgctggccg gcggggggtt gaccgtgagc tgcagctgga cctgcatgtc cgggtgctgt
     9301 tttacgaagc tggcgatcag cggggcgatg tggctgcgcc cgaaacccgg ggtggcgttg
     9361 acgcgcagca ggcccttggg cgccgccacc gcgctggata ccagttgctc catgtcggcg
     9421 atctcggcca gaatgcgccg cgcatgactg aggtagatct cgccttcggg cgtggggctg
     9481 atgcggcgcg tggtccggtt cagcaactgc acgcccagcc gcgcttccat gagcgccagg
     9541 cgtttgctga tggccggtgt ggtcacgtcg agttcgcgcg ccgtggccga gaagctgccg
     9601 caacgcgcca gcaggctgaa aaaagccatc tcggaagggg cggaggcggg tgtgctcatt
     9661 attaaatcaa agtcaacaat gccataactt taaggcgttc tgttgaatcg ggcaagcgct
     9721 agagtccatg cttgttgatg atttcaagga catgtcatga aggtttacaa gattgcctgc
     9781 attccgggcg acggcattgg caaggaagtg gttcccgcgg gtcaacaggt gctgcaggcc
     9841 ctggccgcca cacagaattt cggtttcgct ttcacctcct tgggctgggg cggcgactgg
     9901 ttccgcgcgc acggcgtgat gatgccggac gacggcctgg atgccctgcg cgaccaggac
     9961 gccatcctgt tcggctcggc gggtgacccg cacattcccg accacatcac gctctggggc
    10021 ctgcgcctga agatctgcca ggggttcgat cagtacgcca atgtgcggcc cacgcgcatc
    10081 ctgcccggca tcgacgcacc gctcaaacgc tgtacggcga aagatctgga ttgggtcatc
    10141 gtgcgcgaga actccgaggg cgaatattcc ggcgtcggtg ggcgtgccca tcagggccat
    10201 ccgatcgagg tggccaccga tgtgtcgatg atgacgcgcg ccggcgtcga acgcatcatg
    10261 cggtacgcct tcaagctcgc gcagtcgcgc ccgcgcaagc tgctgaccgt ggtcaccaag
    10321 tccaacgccc agcgccacgc gatggtgatg tgggacgaga tcgcgctgca gatcagcaag
    10381 gagtttccgg acgtgacctg ggacaaggaa ctggtggacg cctgcaccgc gcgcatggtg
    10441 aaccgcccgg cctcgctgga caccatcgtg gcgacgaatc tgcacgccga catcctgagc
    10501 gacctcgcgg cggcgctggc cggcagcctg ggcattgcgc ccaccggcaa catcgacccc
    10561 gagcgccgct acccctcgat gttcgagccc atccacgggt cggccttcga catcatgggc
    10621 atgggcctgg ccaatccggt cggcacgttc tggagctgcg tcatgctgct cgatcacctc
    10681 ggtgaacacg tggcggcgca gcagctgatg caggccatcg aggccgtcac tgccaacccg
    10741 gccctgcata cgcgcgacct gggcggaaac gccaccaccg cgcaggtcac cgccgcggtg
    10801 tgtgggttgc tcgcccagca gggcggcacg cagcaggcgc gcgcggcctg aaaattttcc
    10861 ccgtggcatt cacataaaac aaggagacag cagcatgaaa gttgacatca gacaatggat
    10921 aggcatcgcc tgcctggccg ccgcacccgc cgtcatggcg caagcctgcc cggacaagaa
    10981 cctgcagtac tggcaggctt ttccgcccgg cggcgagtcg gacctgtcgg cgcgccacca
    11041 gcaggtggtg ctgaagaaaa agtgcccggc catcgacacc gtcatccagt acaaggccgg
    11101 tgccggcggc gcgctgatgt ggtcgcagat gaacaccctg cccggcgacg ggctcaacat
    11161 cgtgggtgtg aacctgccgc acatcgtgtt ccagccgatc gaaggcctgg tgcagtacaa
    11221 gacgcaggac atcacgccgg tgttctggtt ccactacaca cccgatatcc tggtggtgcc
    11281 cgagcagagc ccgatcagga atttcgcgga cttcatcaag gcggccaagg ccgaccccgg
    11341 caagctgagc ctgggcggct cgggccagaa ctcggccaac catgcggcgc acgagcgcat
    11401 gaatgcggcc tttggcgtca agaccattta tgtgccgttc aagggtacgg gcgacatggc
    11461 gacctcggtc atgggcgcgc aggtcgacgg tgcaatgacc tacaccgcgt ttgccatcaa
    11521 caacaagacc cgcatccgcc cgctggcggt ggccatggac aagcgccatc cgctgatgcc
    11581 ggatgtgccg acgttcaagg agctcggtgt ggactgggtg gacggtgcct accgcggcat
    11641 cggggtgccc cgcagcacgc cgcccgagac ccgcaagaag atttcggaca tgtgggccgc
    11701 cttgaacaat gaccccgaga tgaaagcgct cgccgccaaa agcggtttcg agctggtcaa
    11761 cgtgggggtg gaccagatgg acgcgttcat gaaggaaaag acccgcttgt acacggacgg
    11821 cgcgcagcgt ctggggctgg gcaagaagta agcggaccgt cgggcccgct gcgctatcag
    11881 gcagcgggtt accggcgggt gacggcggca caatacgggg caggagacat cgaatgactg
    11941 cacccgatcc cgctacccgc ccgcgccagt tcctcgagca tctttaccag gccgcggtca
    12001 cgcgtgccat gccgctgcac aacacggccg cctgcctgcc caagccaccc aaaggcagaa
    12061 cccttgtgat cggcgccggc aaggccggtg gctcgatggc gcaggctgtc gaagcgttgt
    12121 ggccggcgga tgcgccgctg gagggcctgg tcgtcacgcg ttaccaccac attccaccgc
    12181 gccccgaagg tctgccgcag cgcatcgaga tggtcgaggc cgcccacccg gtgcccgacg
    12241 cggcagggct ggccgcctcg cagcgcattc tggccatggt gcaaggtctc acaaaagacg
    12301 atctcgtgtt gtgcctgatc tccggcggcg gctcggcctt gctcacgctg ccggcagaag
    12361 gcttgagtct ggaagacaag cagcgcatca acaaggcgct gctcaacagc ggcgccaaca
    12421 tcgtcgagat gaactgcgtg cgcaaacacc tctcgcgcat caagggcggg cgcctggccg
    12481 cggcctgcgc gccggcgcgt gtggtcacgc tgaccatcag cgatgtgcca ggcgacgatc
    12541 cgtccatcat tgccagcggc ccgaccgtgc ccgacgccag cagttgcgcc gatgcactgg
    12601 ccatcctgaa acgttacggt atcgacgtgc cggcggacgt gttgtcgcga ctggaaagcg
    12661 gggcgctgga aacccccaag cccggcaacc cggcgtttgg cggccatcag gtccacatga
    12721 tggccacgcc gcagcagtcg ctcgaggctg ccgcggctgc ggcgcgtgcg gcggggctgc
    12781 aggcctacat cctcagcgat gaaatggagg gcgagtcgcg cgaggtcggc aaggtgcatg
    12841 cggcgctggc gcgtgcggtg gcgctcaggg gccagccctt cagcaaaccc tgcgtgatcc
    12901 tgagcggcgg cgaaaccacg gtgacggtcc gacccctgca agcgggcgct gccaaaggcc
    12961 gcggcgggcg ggcgggcgag ttctgcctgg gcctggcgct cgcgctgcaa ggccaggccg
    13021 gggtctatgc gctggcggcc gacaccgacg gcatcgacgg tgtggaagac aacgccggtg
    13081 cctttgtggc gcccgacacc ctgacgcgcg cggtggcgca gggcatgaag atcaacggct
    13141 tcctggaccg caacgacgcc tatggctact tcgaaccgct gggcgatctg gtgatcacgg
    13201 ggcccaccca tacgaatgtc aacgacttcc gtgcgctatt gattttgtag ctggctacgc
    13261 aatgtccata aggattgtgg cgagaagagg ttgtcatccc gggcttgatc cgggatccat
    13321 gcttgctgac tactcacgtt gtttatggcg cagcggtttg cccacaagcc ggggggttgc
    13381 ccggcggcaa gtaactttct tttgcgtcgc caaaagaaag tcacca
//
LOCUS       DAIDFG010000048        13356 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_48_length_13356_cov_18.096910, whole genome shotgun sequence.
ACCESSION   DAIDFG010000048 DAIDFG010000000
VERSION     DAIDFG010000048.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 13356)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13356)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13356
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48_length_13356_cov_18.096910"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2504 a         4312 c         4298 g         2242 t
ORIGIN      
        1 ggaatcaaac gatgccagca tgccgcgcag cgcttcgcgc atgtcctggt aggcgtcggg
       61 ggtggggatt tgtgttttca tgagaggttt ttgtggataa ctttgcaaat ggatgtcatt
      121 gcgggcctga cccgcaatcc atgctggcga acctgcagcg gtagtcagtg aggcatggat
      181 cccggatcag gtccgggatg acaggcgtgc gaacgatatt tctccagcct caaaccagcg
      241 tcgccgtggc ctgcatggtg agccagcctt cgtggtcttc ggtccagagc tgcacggtct
      301 tgccgtccgc tgagggtgta ccgtggatcc tgaaaggcca caggtcaaac accggacgca
      361 cggcacgaaa ctcgaacttc agcaggcggg cgcccggcat gtgtttgcgc accagttccg
      421 ccagcagcgt ggcaatcagc gggccatgca cgatgaggcc ggggtagccc tcgacttccg
      481 tgacgtagcg ccggtcgtag tggatgcggt ggccgttgaa ggtcagcgcc gaataacgga
      541 acagcaacac gtcgtcgggt gtaacggttt ccgtccacgc cgcctctttg gcggccggct
      601 gtggcggcgg caccggatca ccaggctgcg ggttggcgcg gtaaacgatg tcgtgttctt
      661 cgctgagggt caggccctgc gcgttgtgga tctcgtggcg gaccagcaca aacaccaggt
      721 caccggtgcg cccggccttg tgggtgaccg actcgatgcg cgaaacccgt ttaaccacat
      781 cgccgacctg cagcatctgc tggtgccagt gcagccggcc cccggcccac atgcggcgcg
      841 gcaggggcac gggcggcagg aagccgccgc gccgggcgtg cccgtcgggc ccgatctcgg
      901 accggcgcgg ctgcggcaaa aagtacagcc agtgccacag cggcggcacg gccgtgccgg
      961 ccaccggcgg cgggtcctcg cggtccagcg tggccgacag cccgcgcagc ggcgcggccg
     1021 tgatgtcgtc caccagggtt tcggtacagc caacccagct ttgcaggtgg gccagcgtgg
     1081 cggcgtctat cgtgcgaatg tcgggcgtca tggtttcaga tggtcgcgca gtgcggcgca
     1141 gaaagcaatc agccatttca acagccagcc ttcgctaaat ccgaagcctt gtatgcccac
     1201 aggccccccg tttttgccat tttccattgg aaacagcccg ggacaaggct tcatcgaaac
     1261 ggaaggcttg tctgacttgc ggtagcatgc tgtcatgcaa ttcgacctga cggacctgcg
     1321 gctttttgtg gcgaccgccg acgaaggcaa catgacccgc gccgccgaac gccagcacct
     1381 ctcactggcg gcggcgagcg cgcgcatcaa gtcgcttgag gcccagtcgg gcctgagcct
     1441 gctctaccgc gaggcacgcg gtgtgcgcct gaccccgcct ggcgaggcct tcctgttcca
     1501 cgcacgcggg gtgctgcggc aagccgagca actgcgcacc gacctgcagg aatacggcgg
     1561 cggcttgcgc ggtcatttgc gggtgtttgc caacaccacc gcggtgacgg actttttgcc
     1621 ggagatcctg ccgggctttc tggcggacaa cccgaaaatc aacatcgacc tgcaggaaaa
     1681 acccaatccc gagattgcgc gcggtgtgct cgacggccgc gccgacatcg gcatcgtggc
     1741 cggccaggtg gacacgctgg ggctggaggc gattcacttc agcaccgacc ggctggtgct
     1801 ggccacggcc agaaaccacc gctttgcgcg ccgcaagaaa attgcctttg ccgaaacgct
     1861 ggacgaggac gctgtgggca tgcagtcggg cagcacgctg cagactttcc tggcgcaggt
     1921 ggtcgagcgc ctgggcaagc cgcagaaact gcgcatccag ctctcgagct ttgacgcgat
     1981 gtgccgcatg ataggtgcgg gcgtgggtgt cggcatcgtt ccggaatcgg ccgcgcggcg
     2041 caaccaggag gccgtgggca tcgcgctggt ggaactgctg gacccctgga gcgtgcgcga
     2101 gcgctacatc ctggtgcgca agcgcgatgc gttgccgcgt tatgccgagt cgctgatcga
     2161 gacgctgagc gcgcactacc gcgaccaggc ttgaaagcgt ctcctaggaa cctttccaga
     2221 ccgggcttct tttcgccaca aacgccgaca caccctcgcg aaaatcgtca ctgccgtaac
     2281 agcggcggat caggtccgcg tcggaaggca acccatggct gaccagccgg cgcaggcctt
     2341 ccttgctgac ccgctgggtc accggcgcca gggcggccag gcgttcgcac agactcgcca
     2401 gcaccgcatc gatctcgcca gcctcgctga tctgggtgag aaacccgcag gccagcgcct
     2461 cgtcggccgt caacacctcg gccagcatca gcatgcgttt gacgcgggga atgccgaaca
     2521 cggcgctgag ccgcgccaga ttcgccaccg acaggcagtt gcccagggtt cgggcaatcg
     2581 gcacgccaaa acgcgaaccg ggtgtggcga tgcggaagtc gcaggccgtg gcgatcgcca
     2641 ggccgccgcc tatggtccag ccttcaatgg cggcgaccgt cggcatgggc agcgactcca
     2701 gccgggcaat gcattcgtca atctgctgct cataggccac gccgtcctcg ccgctgtcga
     2761 aagtggtgaa ctgctcgatg tcggtgccgg ccacaaaggc ctggccgccg gcaccgcgaa
     2821 aggtcaccac gcgcaccgag gtctcggcgg ccagctgctc acagatctgg ttgagccgtt
     2881 cgtacatggc ccaggtcatg gcgttgcgcg ccgcgggacg gtcaaacacc accgaggcca
     2941 cacccccgct gatgctcagg tggacggcgc cttcactcat gctgcaaaag ccccctgctc
     3001 gccgagccgg acctgcgcct cggcgtcgat gcccagttca gccagcacct cggccgtgtg
     3061 ttcgcccagg cgcggcggat ggcggcgcac ctgctgaggc gtgccggaca tcttgactgc
     3121 gaagccgatg ttgggcacct tgccttccac cgggtggtcg atctccatgc gcatctcgcg
     3181 cgccttcgca tgctcgctgc caaaggcttc gggataagtc aggatggggc cggcggggat
     3241 gccggcctcc agaaaggtgc tgacccagta atccttgggc tggaggcgga aggatttctc
     3301 cagctccact tccagcgcat gccggttcgc caggcgcaac gagatggtgg aaaagcgttc
     3361 gtcggccagc agttcctcgc gacccatgag cttgcacagc tgcagccaca gcttctggtt
     3421 gttcgcgccc atcacgaagt agccgtccga gcaggccatc gcctggtaag gcgcggtcat
     3481 cttgttcgag gtgcccagcg gttcgggctc cttgcccgtg ccccagtagt cgcagatgtc
     3541 ccagaccgaa aaggccagcg ccgaatcgaa cagagaggca tccacatgct ggccctggcc
     3601 gctggctttg gcgccgatgt aggccgacag cgtggcatac accgcgaaca gcgcgcagcc
     3661 gatgtcggcc accggcacac cggcttttac cggcggcccg ccgggataac cggtaacgct
     3721 catcacgccg gacatggcct gcgccatcag atcgaacccc ggccggtcgg cccagggacc
     3781 ggtctggccg aagcccgaga tgctggtgta gaccagacgc gggttgattt ccttgagcac
     3841 ctcgtaaccc aggcccagct tcttcattgc gccggggcgg tagttctcca gcaggatgtc
     3901 ggcatctttc accagccgca gcagcaaggc cctgcccgcc tccgacttga ggttgagcgc
     3961 gacgctgcgc ttgttgcggt tcatgttcag gaagcccagc gaatcgggac ccttcatctt
     4021 gaagcccatg gcgccgcgcg tctggtcgcc gctgcccggc ggctcgatct tgatgacgtc
     4081 ggcccccatg tccgccagca gcatgcaggc gtagggaccg gccatgacct ggctgatatc
     4141 gagcacgcgg acgccggcca gcggcagcgg ccgcccgcct gcagcgcgtt ttcctgggtc
     4201 ttgcgtcaaa acatcgctcc tcaacggtag aacagctcga ccaggcccag gcccgtcacc
     4261 ggcacatagg tcaccagtat caacacggcc accagcacgc caatgaacca gatgttgacc
     4321 ttgctgacat cccagaccga ggccttggca atcgagctgg ccaccatcag cacactggcg
     4381 accggcggcg tctgctgccc gatgcccagg ttgatggtga caatcaggcc gaaatgcacg
     4441 ggatcaatgc ccacggagcg gaccaggggc atcacgatgg gaatcaccag aatgatggcc
     4501 gcggctgaat gcaggaacat gccgaggaac aggaagagca cgttgagcag cgccagcacc
     4561 acccacttgt tggaagtgaa gtccgacacc gccttggcca gggcctgtgg cgcctgcact
     4621 tcggtcaggt aggtgcccag caaggcactg gtggccacca gcagcatcac cacggcggtc
     4681 tgcacgccgc cctccatgac agccgcgcgc agatgcttga ggtccagctc ccggtacacc
     4741 accagcccga tgaacagtgc cgcaatcacg gccagcgccg caccttcggt ggccgtgacc
     4801 acaccgccaa agatgccgcc caggatgatg atgggcagtg caaaagccca gagcgcctcg
     4861 cgcccggttt tggcgacccg cttgatggag aacgcatctt cgcgcggcag gttgtagcgg
     4921 cgcgccagaa aactggccat gcccatcagg ccggcggcac cgatcaggcc cggcacgatg
     4981 ccggcgacaa acatctgcac gacggaggtt tccgccatca cggcgtacag gatcatgggg
     5041 atggacggcg gaatgatgac ggccagcgtg gccgacgacg acatcacagc agcggcaaaa
     5101 ggcgcggggt agcccttctc tttcatgccc ggtatcagga tggagccgag cgcagcaaca
     5161 tcggccactg cagaaccgga gatttccgcg aaaaaaagcg aagcgccgat ggacacatgc
     5221 gagagaccac cccgcaccca gccgaacagt gccgacgcga aggcaaccag gcgctgcgaa
     5281 atgcccgagg cgttcatgat ggcgccggcc agaatgaaaa ggggaatggc cagcagcggg
     5341 aaattggtcg ccccgttgta caccaccagc gccacattgg gcagcgaatc gaggccatgg
     5401 cttgtcagca tggcgatcac tgccacgatg cccagcgaca cggcaatggg aatgttgagc
     5461 aacaccagcg ccagcacggc accgaacagg atcagcatga tggtcatggt ttttattccg
     5521 tgctttgcat ttcggagacg gggacggcag ccggcggatg ggctgcaggc cgttcagcga
     5581 tcaggtcgac cagaaacatg acttcggcaa ccagaatcag cgccgaggtg agggggatga
     5641 cggactgcac gaaattcatc ggcacccacg gcaggctcac cagcgcatcg cccgccaggg
     5701 ccggcatgat ggtcagaccg acccagccca gcagcgcgaa aaacaggatc accaggatct
     5761 ggcccaagat gctgaccacg cgcttgaccg cgggtggaaa ctggtccacc acctcggggc
     5821 agccgatatg cgcgcgtttg accgaggcca gtgccgaccc gtaaaacgtg agccaggcca
     5881 gcagcaccga ggcaacctcg tcgtaccaga ccagggagtg cccgaccatc cggaacacaa
     5941 tgcccagcgt gacctccacg gccagggcaa tcacgagaaa catcacgacc cactcgagaa
     6001 gcaggccata acggttccgg aagcgatgca tcgcgccgcc gggagccagt gccgaagagt
     6061 tttgcatcag gaaccgccct tatttgccga gtgcgacggc tttgtcgatc agcggtttgg
     6121 cgccagagac atccttgcca aattcttcat aaatcgcctt gctggcggcg atgaaggcgt
     6181 ccttgtcgac ttcgttgacc tggatgccgc tttgcttcag cttggcgagc aggtccgtgt
     6241 cgtccttggc agcggtggca tggacaaaag cctgtgtttc gcgggccgtg tcttccagca
     6301 ccttgcgcac atcggccggc aaggtgttgt agcggcgcga gccgacggtc aggtaggccg
     6361 gcgtatagac atggccggac agcgagagga acttctgcac ttcatgcagt ttggccgagt
     6421 agatctgggt gaacgggttt tcctgcccgt ccatcacgcc ggtctgcagc gcagtgaaca
     6481 gctccgagaa cttcatcggg ctgggattcg caccgtagtc ctggaacatt ttcacccgcc
     6541 atttgccctc aggcacacgc agcttgatcc ccttgaggtc gtccggtccc ttgatgggct
     6601 ttttgttgtt ggtgatgtgg cggtagccgt tttcccagac ggccagaacc ttcaggccct
     6661 ttttctcggt ttccggtgcg atgctgggcc agaacacgtc tttctcgatg cggatcatgt
     6721 gcgccctgtc cttgacgata tagggcattt cgaaaatgcc gaacaggtcc acctcggagg
     6781 tcatcacggt cgacggcagg gccatgtcga tggtgccgag cttgagcttc tggatcatct
     6841 ccttgtcacc gccgagttgg ctggagccaa aggtcacgac cttgaccttg ccggcaagtt
     6901 tggcgttcac gcgtttggcg tactcgtcgg cactgagctg gaacagcgag ccaggctcgc
     6961 cgacatggcc gagcttgagc tcgaccgcct gtgccagcac ggcgccatgc agggccagtg
     7021 ctgcgcttgc aacaagcagg gatcggcggg tggccgcaga aaatgtgaac gctttcatga
     7081 tgtctccttt tattgctatg gatgtccgtg gcttgcaaca gaccacacgg ccggacagcg
     7141 tgtggcggaa aaaaagatgt cgggtcaggc ggctttctgc agcggaaggg ccaccgggtg
     7201 gcctgcgaag tagtccatcg ccgcctgcac gcccgaaccg gccagcttga tgccggcgag
     7261 cttgagcccc atttcgcagg cgcacagggc cgccatcagg gtcaggtcat tcgaatcgcc
     7321 gaggtggccg atacggaaca tgcggccctt gaccttgccc aggccggtgc ccagcgagca
     7381 gtcaaaacgc tcgtggatga tccggcgcac ggcgtccgcg tccacgcctt cgggtgtcat
     7441 cacaccggtg aggacaggtg aatacacgga cgcatcggcg cattggatct gcagacccca
     7501 ggccttgacc gcggcgcgca cgccttcggc ccagcgcagg tggcgcgcaa acaccacgtc
     7561 cagcccgtgc tcgagcagca tgtcgcaggc ttcgttgagg ccgtagagca ggttggtgtt
     7621 gggcgtggac ggccagtagc cggttttgtt catctcgatg atttcgtccc aggcccagaa
     7681 ggccttgggc agcctggcgg tcttgccggc ttccagcgcc ttggcggaca ccgcattgaa
     7741 gctgatgccg ggcggcagca tcaggccctt ctgcgaaccg ctgatgctga cgtccacacc
     7801 ccattcgtcg tgccggtagt ccgccgaggc caggccggaa atggtgtcca ccagcaacag
     7861 cgccggatgt ttcgcagcgt caatggcctt gcgcacggcg gcgatgttgc tggtcacgcc
     7921 ggtggaggtt tcgttgtgca ccacgcagac ggccttgatg ctgtgctggg tgtcggtctt
     7981 gagccgctct tcgatcaggt cggcgcgcac accgtggcgc cagacttcgg cgccggggtc
     8041 ggtcatcacc tcggttttca ggccgatgcg ggtggccagt ttttgccaca gggcggcaaa
     8101 gtggccggtt tcgtacatca gcacgtggtc gccagcactc agcgtattga ccagcgcggc
     8161 ttcccaggcg ccggtgcccg aggccgggta aatgatgacg ggctggctgg tcttgaagat
     8221 ttttttgatg tcggccagca ccttcaggcc cagcgcgccg aactcgggac cgcggtggtc
     8281 gatggtgggc aggctcatgg cgcgcaaaat gcggtcaggc accggtgaag gaccggggat
     8341 ctgcagaaaa tgacggccgg tagggtgaaa atcgagtgtc agcatgattc ggcaggcttc
     8401 gtgaacgttc cccatttttt gcatacaaaa ttcatttgtc aagggggtct ctcctataat
     8461 tcaaattgac agccaacttt tttgcataca aaatttcact atgagcgccg acgtcatatc
     8521 catcccacgt gccgccctgc atgagcaggt ggcgcaccgc ttgcgccaga tgctggtcga
     8581 aaaccgcatc ccgccaggag ccaaactcaa tgaacgcgaa ctcagcgagg tgctcaaggt
     8641 gtcgcgcacg cccctgcgcg aggccatcaa gatgctggcc gccgagggcc tggtcgaatt
     8701 gctgccgaat cgcggtgcga ttgcggtcga gctgtccgaa gccgatgtac tcaacacctt
     8761 tgaggtgatg tccagcctgg aagcgcagtc gggggagctg gcggccgagc gcatcaccgc
     8821 cgccgagctg gccgagatca aggccatgca ttacgagatg ctggcggcct acacccggcg
     8881 tgacctgccc gcctattacc ggctcaatgc cgccattcat ggcgccatca atgccgccgc
     8941 caaaaacccg gtgctcaccg ccacctacaa acaggtcaat gcacgcttgc aggcactgcg
     9001 tttccgctcc aaccaggacg gtgaaaaatg gagcgccgcc gtgagggaac acgaacagat
     9061 gattgaggcg ctgggcacac gcgatgctgc tgccatgcgt gccgtgctgc tggcccacct
     9121 gaaaaacaag cgcgacgtgg tggtcgagca gttgcgcgcc gtacagcagc aggccaaggc
     9181 atgaacgcac catgaacgcc cctctgcccc tgaaagtcac gcaggacacg gcagcccacg
     9241 cctacgacag cgtcgcccgg atcagcaacg aggtggccgg ccggctggcc gtccggctcg
     9301 ccagcgaaac ccgtggcgag gtgctttttt ccgccgcgga ccgcggccgc tacgccaccg
     9361 atgcgtcgat ttaccaggtg atgccggtcg gcgtttttgt gccgcgcagc gcgcctgacg
     9421 tgaaaaccgc gctcgacatc tgccgcgaca tgggcgtgcc catagttcca cgcggcggcg
     9481 gcaccagcca gtgtggccag accgtgggcg ccgggctggt catcgaccat gcgaaacaca
     9541 tacgcaacat catcgatgtg gatgtggaca agcggaccgc cgaggtggag cccggcatcg
     9601 tgctggacca cctgaacgcc gccctgaaaa aacacggcct ctggtacccg gtcgacgtgt
     9661 ccaccagcgc gcaggccacg ctgggcggca tggccggcaa caactcctgc ggcagccgca
     9721 gcattgccta cggcaacatg gtgcacaacg tgctgagcgc gcaggcctgg acctcggaca
     9781 gccagttgct gcagctgggg ccctacgccg ccagcagcgg caaggcacgt gagctgggca
     9841 accacgttcg ctcgctggcc gaggagctgc acccggccat caaccggatg tggcccaagg
     9901 tcatgcggcg cgtgggcggc tacaacctgg acatcttcga cccgcagagc gagcgaccct
     9961 acaccagcga cggctcggtc aacctgtcgc acctgctgat cggcagcgaa ggcacgctgg
    10021 cgctgaccaa atcactgaca ctgaaactgg ccgaactgcc cagggccaag gtgctgggcg
    10081 tggtgaactt tccgaccttt taccgcgcca tggatgccgc gcagcacatc gtcaaactcg
    10141 gaaatggcag cctgacggcc gtcgagctgg tggaccgcac gatgatcgac ctgtcgctgc
    10201 agaacccggc ctttgcgccg accattcgca gcgcgctgat cggccagccc gcggccattt
    10261 tgctggtcga gttttcgggc accagcaagg ccgacctgtt gccccacctc aagcgcctgg
    10321 gcgaactgat gggcgatctg ctgctgcccg actcggtggt cgagatggtg gacgacgccc
    10381 cgcagaaaaa cctgtgggag gtgcgcaagg ccggcctgaa catcatgatg agcctcaagg
    10441 gcgacggcaa gccggtcagc ttcatcgagg actgcgccgt gccgctggaa cacctggccg
    10501 aatacaccga cgcattgacc gaggttttcc gaaaatacgg cagcaagggc acctggtacg
    10561 cccatgcctc cgtcggcacg ctgcatgtgc ggcccattct cgacatgcgg cgcgagggtg
    10621 cgcccaagat gcgcgcgatt gccgaggaag cgtccgagct ggtgcgcaag ttcaagggcg
    10681 cctacagcgg cgaacatggc gacggcctgt gccgcggcga gtggatccag tggcagttcg
    10741 gcccgcagat caatgcggca tttgcggcca tcaagcaggc catggacccg atcaatctgc
    10801 tgagcccggg ccgcatgatc aacccgccga agatggacga tacgcggctg atgcgttttg
    10861 cccccggcta caaggtcatc ccgatccaga cagcgcttga ctggcgcgcc tgggacgtgc
    10921 agaacgaccc ggtcaccgag ctgaccagcg caccgggcag cggcggcgac ccggcccagg
    10981 gcctggccaa ggccgtggag atgtgcaaca acaatggcca ctgccgcaag ttcgacgctg
    11041 gcaccatgtg ccccagctac cgcgtgacgc gcgacgagca acacctgaca cgcggccgcg
    11101 ccaacacctt gcgcctggca ctgtcggggc aactcgaagg catgaatgcc gccgacgcct
    11161 tcaccagcga agcggtcaag gacgcgctgg acctgtgtgt gggctgcaag ggctgcaagc
    11221 gcgactgccc gaccggcgtg gacatggcca agatgaaggt ggagtttttg caccactaca
    11281 aggccaggca tggctacacg ctgaaggaca agctggtcgc gcgcctgccc gactacgcgc
    11341 actgggccag ccgcctggca ccgctgctga acctgcgcga caggattccc ggcctggccg
    11401 cgctcagcga gaagttcacc ggcttttccg ccaagcgcac cttgccccag tggacaacgc
    11461 acaccttttt cagcacgcca tttctgaacg ccacgcgaca ggaggtgttg gctgcggaga
    11521 aaccagtcgt tttgttcgtc gacaccttca acggcacctt tgaacccagc aacgcagttg
    11581 ctgccttgaa agtgctgcaa gccgccggtt acacggcgca tgtcgccgtc aaaaaagcag
    11641 cagatgggaa acacctgtgt tgcggccgga cctacctggc cagcggcatg gtcgatgaag
    11701 cgaaggccaa ggccagggag ctggtcgccg cattgctgcc ctttgccgag cgcggcatcg
    11761 ccatcgtcgg cctggagccg tcctgcctgc tgacgatgcg cgacgagatg ctggtgatgg
    11821 gcctgggtga agcggctcaa accatcagcg accaggccct gctgtttgaa gaatttctgg
    11881 cgcgcgaggc cgcagccggc aagctcgacc agctcaaagc cagactgcag ccggtggacc
    11941 aggccatcct gctgcatggt cattgccacc agaaggcgtt tggtgcggtc agccccatca
    12001 tcgatgtgct caagctgatc cccggcgcta agcccgagct gatcgagtcc agctgctgcg
    12061 gcatggccgg cagcttcggc tacgagacca gccattacga ggtgtccatg caaatggccg
    12121 aactgagcct gctgcccgcc gtgcgccagc agcccgatgc catcgttgtt gccgacggca
    12181 ccagttgccg gcaccagatc cgcgatggcg cgcagcgcga agccattcac gtggcggtgc
    12241 tgatggcgca gcaattgcgg gcctgacgcc ggctccaggg gcatggtccg gccggagtac
    12301 agtggcagcg atgcgcaccc ctgacattct gaccctcacc atgaaccctg ccctggacgt
    12361 gtcgacgtcc acggacaagg tcacggacac acacaaactg cgctgcgctg ccgcgcaata
    12421 ccatcccggc ggtggcggca tcaacgtggc ccgcgtgttg cagcgcctgg gcagcaactg
    12481 cctggcgctg tatccgaccg gcggcatgaa cgggcaaagg ctgcgccagt tgctggacca
    12541 ggagcaggtg tccagccagt gcctggccat cgccggcgag acacgcgaga gttttcatgt
    12601 ccacgaaacg gccagcgggc aggacttccg ttttgtcctg cccggccctg ccctgcgcgc
    12661 gaccgagtgg caggcctgcc tcgatgttgt tggcgctctg gcagcggcgc cgtcctggct
    12721 ggtggccagc ggcagcctgc cacccggcgt gccggctgac ttctatgcgc agctcacacg
    12781 gctggcgcgc gcgcgcggca gccgcgtggt gctggacagt tcgggcccgg cgctcgcagc
    12841 ggcgctggcc gaaggcgtgc acctgatcaa gcccagcctg cgcgaactgc gcgaactcac
    12901 cggcctgccg ctggagaccg agacgcagcg gctggccgcg gcccgcacca tcatcaccgc
    12961 cggacaggcg cagatcgtgg cgctgtcgct cggcgaggaa ggcgccttgc tcgtcacggc
    13021 cgagcaggcg ctgcgggcca gcgccgtgcc tgtcgaggtg gccagcacca taggcgcggg
    13081 cgacagtttt gtcggcggcc tggtctgggc actgagccgg cagaccgatc tggcgcaggc
    13141 ttttcgttac gccatggggt ccgccgcagc cgccctgttg tcggcaggca ccgcgctgtg
    13201 ccagccggcc gatgtggaac gccttgccag ggaagtgatc gtcacaccgg ccggtgcttg
    13261 atctggcgca aggcagcagc gacggccggc gtctaccgta gggaagcgct gaacaagtcc
    13321 cctgcggcgc gcgatcagcc gggctcgggc ggtctg
//
LOCUS       DAIDFG010000049        13303 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_49_length_13303_cov_18.548158, whole genome shotgun sequence.
ACCESSION   DAIDFG010000049 DAIDFG010000000
VERSION     DAIDFG010000049.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 13303)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13303)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13303
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49_length_13303_cov_18.548158"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2420 a         4480 c         4070 g         2333 t
ORIGIN      
        1 gccctgggca ctggccgccg tcgtcgccaa ccacgcgctg gtggccggcg tcggcctgtg
       61 gccgcgcagc cactggctgg gcccgaactg gtcgcggctg cccgccgccg cgatggccag
      121 gaacgaaatt gccctgacca tcgacgacgg acccgacccg caggtcacgc cgcaggtgct
      181 ggacctgctg gaccgctacg ccgcccgggc cacgtttttt tgcattggtg aaaaggccgc
      241 gcgttacccg gagctgtgcc gggaaatcgt tcgccgcggc cacgcggttg aaaaccacag
      301 ccagcaccat cgccaccact tttccgtcat ggggccgcgc ggcatggcgc gggagctgca
      361 ggccgcgcag gacacgctga gcgcaatcac cggccggcga ccgctctttt tccgcgcgcc
      421 cgccggcctg cgcaatcctt ttctggaccc ggtactcact cgcatgggac tgcggctggc
      481 cagctggtcg gtccgtggtt tcgacacccg ggtcggcgat gcgcagcgcg tcaccggcaa
      541 gctgctgcac ggcctgaagg ccggcgccat cgtgctgctg cacgacggca atgccgcgcg
      601 tacgccaggc ggcatcccgg tgattctcga agtcctgccc accgtgctga cctcggctgc
      661 ggcggcccgc ctcaagccgg tgaccctgcc cgaggccctg gcatgagcgc gcccccggtc
      721 agccctgagg ccctgccctg gtaccgccag gccgcacggc ttgtgccccg ggacgtggtc
      781 ctcaaaagca taggtatccc gctgttcatc gcggtgtttt ttgccgccta tttccatgtg
      841 ctcaacaacc cggcctatcc cgtcacggtg atgccggtca catggctgga ccgcttgatc
      901 ggctttgaac ccatggccct gtcgctgtat gtgtccctgt gggtgtatgt gtcgttgcca
      961 ccggcgttcc tggcgagccg gcgcgaactc tatctgtaca gcctggccat ggccggcacc
     1021 tgcctgaccg gattgatcat tttctatttc tggcccacga ttgttccggc ggctgacatc
     1081 gactggagtc tgtacccggg cgtggacttc ctgaagaaca tggacgcagc gggcaacgcc
     1141 tgcccgtccc tgcatgtcgc caccgccgct ttttcgggct tctggatgca tcacctgctg
     1201 cgccgttttg gcgcgccgcc gtgggcctgc ctggccaatg cactgtgggg cgtcggcatc
     1261 gtttactcca cggtggccac acgccagcat gtggccgtgg acatgtgggc cgggctcctg
     1321 ctggggaccg tcaccgcctg gttgtccctg cgccacgtgg tgcgtacccg ggccgaccag
     1381 catcgcccct acctcgctgc ctgacaaccc atcaccgcaa aaaacgccca ctggatttgc
     1441 taacatgact ttacttttag atcggctaga tttcttctga acccgctctg gctctcccat
     1501 ttcaccgcga ccagcagcat cggtcgcggc ctccagcaaa ccctcagcgc cttgcgcgag
     1561 cagcgtggcg gccttgtgcc ctgcgcgttt gacacggtcg atctgcccac ctttgtcggc
     1621 gaagtgacgg gggtggatgc cgtgacgctt cccgacgcac tggcagcgtt cgactgccgc
     1681 aacaaccgcc tcgcgctgct gggcctgatg caggacggtt ttgccgaagc cgtcgcagcc
     1741 gccagccaga agtacggcgc gcagcgcatc ggcgtcttca tcggcaccag cacctcgggc
     1801 attttgcaaa ccgagctggc ctaccgccgg cttgatccgt ccacgggcgc ctttcctccc
     1861 gatttttcct accggaccac gcacaacacg ttctccgtgg ccgatttcac gcgccatgtc
     1921 ctggccctgg ccggtccggc ggtggtggtt tcgtccgcgt gttcgtccag cgccaaggtg
     1981 tttgcttcgg cgcgtcgcat gatggcggcc ggactgatcg acgcagcggt ggtcggtggc
     2041 gtcgattcgt tgtgcctgac gacgctgtat ggcttcaatt ccctgggcct gacttccacc
     2101 cgggcctgcc ggccattcga tgtcgaacgc gacggcattt ccatcggcga agcggccgcc
     2161 ttcgcgctgc tcgaacctgt gccggagcat ctgaacaagg acgccgtgct gctgctgggt
     2221 atcggcgaat ccagcgacgc ccaccacatg tcctcgcccc accccgaggg cctgggcgcg
     2281 cgcatggcca tgcaggacgc cctggccacg gccgggctgg acgcagcgga catcgactac
     2341 atcaacctgc atggcacggc cacgccgagc aacgacgcgg ccgaaaccag ggcggtggcg
     2401 gcgctgttcg gatcagccac gccctgcagt tccaccaagg gcgccaccgg ccacaccctg
     2461 ggtgcggcgg gtgggctgga ggcggtgatc tgcgcgctcg tcctgcagca cggcctgttg
     2521 ccagctggcc tgaacacacg gcaactggac ccggccctgc ccctgaacta cctgctgcac
     2581 aaccgcgagc aggccgtccg ccgcgtgctg agcaattcct ttggtttcgg cggcaccaac
     2641 tgcagcctga tttttggccg tgccgggtaa gacgacgatg acacatcctg catccgctgc
     2701 aacgtccctc acggtttatg tcgaaggcat cggcctgctg ggcccgggcc tgtcgggctg
     2761 ggcgccgggc cgcgaacagc tggccggcgg cgctccttac gaggcggcgc gctgcgtgct
     2821 gccgctgccc atggccctgc ccccggccga acgccggcgt gcgggcgccg tggtcaaggt
     2881 ctcgctggcc gtcggccagg aagccgtgac tgcctcgggc ctgtccacag ccaggctgcc
     2941 ctcggtgttt tcatcgtcga gcggcgacgc catcaattgc catgaaatct gcaaggcact
     3001 ggcatcgggc gacaggctga tctcgcccac gcgttttcac aactcggtgc acaacgcttc
     3061 ttccggctac tggagcatct ccagcggctc catggcgaca tcctccgtgc tgtgcgccca
     3121 cgatggcagt tttgcagccg gcctgcttga agcgatgacg caggcggtgg tcgatcagac
     3181 ggccgtcctg ctggtcgcct atgacaccga ctaccccgaa ccgctgcata gcgtgcgacc
     3241 cctgccggac tccttcggcg tggccttggt gctggccccg caacccagcg agtgcagcct
     3301 ggccaagtgg accctggccc cagccacctg cctcacaggc gccagcgcaa ccgccatgac
     3361 cggcagcgcg ctggagcagc tgcgcgccag cattcccgcg gcccgctgcc tgccgctgct
     3421 gcgcagtgtg gccacgcagg aggcaggtga gatcgtgctt gactacctcg atggtctgca
     3481 gctggccgtg caggtgagtc catgctgagc gccgcgcatc ctcccgccac gctgcgacac
     3541 gaggacatcg cgcgccgcat cccccatcag ggcgccatgt gcctgctgga cagcgtcagc
     3601 acctgggaca cccagcacat catctgccag gcgagcagcc accacgcgcc cgaccatccg
     3661 ttgcgggccc atggccggct cggtgcggcc tgcggcgtgg agtacgccgc gcaggccatg
     3721 gccgtgcacg gcacactggt ggcccaagcc ctggccggcg cgaacactgc caacacgccg
     3781 ccgcgcgccg gctacctggc cagcatgcgc agcgtcacgc tgttcgtcga acggctggac
     3841 acggtcgcag gcgacctgag catccgcgcc gaccggctga ccggcgacga caacaccgtg
     3901 ctctacagct ttgccgtgca ggccggcgac cagcccctgc tcagcggccg cgccgtggtg
     3961 gtgctcgatg catcggcgct ggcgctgccc ccctccaccc caccgcccgc agcggcagcg
     4021 agtcccccat gacccccccc ctcaaacgcg cactggtcac cggcggcagc ggcggcctgg
     4081 gctcagccat cagccggcgc ctggcggccg atggctttgc cgtcatcgtc cacgccaaca
     4141 gccacctggc cacggcgcag gcggtcgccg ccgacatcgt ggcccagggc ggccaggccc
     4201 aggccgtggc tttcgatgtg accgacgccg cggccaccgc ccgggccctg gaaaccctgc
     4261 ttgaggacgg ccccatccag gtgctggtca acaacgccgg catccatgac gatgcggtgt
     4321 ttcccggcat gcagctggcc cagtggcagc gcgtgctgga tgtctcgctc aatggttttt
     4381 tccacgtcac ccagccgttg accatgccca tgatccgcac ccgctggggc cgcatcatca
     4441 ccatcacgtc agtcgcggct gtggccggca accgggggca ggtcaactac tcggccgcca
     4501 agggtgccct gcatgcggcc accaagtcac tggcgctgga agtggcgagc cgcggcatca
     4561 cggtcaacgc cgtggccccc ggcatcatag acaccgccat gacccagggc gccttcagca
     4621 aggacgtcat cgaccgcatg gtgccgatga aacgtgccgg caaagccgag gaagtcgccg
     4681 acctcgtcgg ctttctggcc tcggaacgtg cggcctacat cagcggccag gtcatttcca
     4741 tcaatggcgg catgatctga cctgaaaagc ggctcttgtc tgattgacgg acaaaaaaaa
     4801 tcacttgaaa ctggctgatc cgggatgcag gggttttttc agggtcggga ctcccgcctg
     4861 ataaaaaaac cactccggtg cagcgctggc cgtgttgcac tttccggcag gcatcgacct
     4921 gcctcatcgt cgcaccttgc caacgcacac ccacggcggc ccacgggcgc gtcctactgc
     4981 cgtttcaggt ttaaaggtca gattcatgaa cacttccgaa caggccaacg tggtgcatgc
     5041 gccccacgta ccgttgaccg actactacaa gaccgagcag gacaggcaag cctatttacg
     5101 ccggattttt gacaacaccg ctgccgacta cgaccgtatc gaggccatgc tggcctgggg
     5161 taccggttcg cggtatcggc gccaggccct cgttcgcggc ggcctgaaag cgggcatgcg
     5221 ggtgctcgac gtgggtgtgg gaaccggcct ggtggcggcg caggcctgtc ttctggccgg
     5281 cgacccggcc ctggtcaccg ggattgatcc aagccccggc atgctggcgg ccagcaagct
     5341 acccaggacc atggtgctga tggagggccg ggccgaatca ttgcccttcc cggacaatca
     5401 ctttgatttc ctgagcatgg gttacgccct tcgccacatc agcgatctgg gggtggcgtt
     5461 tgcagaattt caacgggtat tgaaacccgg cggccgcctg tgcatcctcg aaatcaccca
     5521 ggccaaaagt ggtctgggac agtgggtcct caagacctat atgcgcagcg tgataccgtt
     5581 gctgacacgg tttgtttccc ggcaaaaaga gaccgcaacg atctggcgtt actactggga
     5641 cagcatagaa gcctgcgtcc cgccggaaca agtcatggct acactgggcg ctgccgggct
     5701 ggtgcaggtc aaacaacacg tggaagttgg cgtgttttcc gagtatcagg caatcaaacc
     5761 ctagttgcag tcccgtcggt tcacggcctg tcgcagagaa gcggacgccc tgagcgctgc
     5821 cactcaagcg cgaccggacc agcgccgtgc cagcagcacc ggcaggcgca gcacaaaacc
     5881 gaaaaacagg cgcgagtgca tccaactcag cagcaggtta tcgcgcaggt aattgaagtg
     5941 agacacgccg ccttcgtcgg gccggaagta tttgacggtg gccggtaggt tgacgggctt
     6001 gacgcccagc cagcacatgc gcaccaccgc ctcggggtca aagtcaaagc gccgcatcca
     6061 gcgctggccg tgcatgacgc ggcgcagcgg cgcgatcggg taaatgcgaa aaccgaacag
     6121 cgagtcgccg atgccggccc acagcgtttc caggttggcc cagccattgg aaaccttgcg
     6181 gccattgaca cgcaggcgcg gcgcgtcggc cgcaaacacc ggcatgccca gcaccatgac
     6241 ctcgggttgc gcgctcgacg cggccatgaa tggcggaatc aggtgggccg ggtgctggcc
     6301 gtcggagtcc atggtcagca catgcgtgaa accctgcgcc gtcgccaggt ccagcccgtg
     6361 cagcaccgcc gcgcccttgc ccacgttgtg cggcagcacg atcacgcgca agccctcatc
     6421 ctgcgccgcc atggcctgca ggccggcagc actgtcgtcg gtgctgccgt ccaccacgac
     6481 ccagaccggc gtccactgag cgcgcgccgc gcgcacggtt tcatagacct tggggccggg
     6541 gttgtagctg ggtatcagga ccagatgggt acgggacgcg ccgggagcag aaacagtcat
     6601 cgtgtgggag cctggacaag gggcgccagg ccacgatgct caggccggcg gtgaaacggg
     6661 gaaaccgggc aactgcgcgt gcgccagctc ggtctggaaa tactgctcga gctcgctgac
     6721 gaaacgcgcc gtgtgctgcg gcggatcaaa acgcttgccg agacggaccc ggtaagtgat
     6781 gggcatttta ggggctcgca gcaaaggcca gcctttgccc aggaaaccgg agctggtttc
     6841 aataaacacc gtctgcactg gcacccccgc acttttggct atcagcccgg tcgtcccctg
     6901 caaggggccc acgggaaacc gcgtggtgcg tgtaccttcg ggaaacagca gcagatggct
     6961 gccctggtgc agatcgtcca ccgccagctg caccatggtg cgcagcggcg tgttgcggat
     7021 gtagccggcc agccgcgcac ccgcgccgta aaaaacactg ttgacgagtt cggccttcat
     7081 gatgcagccc gcaccgggca ggcgcgacag aatcatcacc gcatcgagca gcgacgggtg
     7141 gttgggcgcg atgaccatgg gcgcttcgcc ctgcaagccg tccagttccg agaggtcgaa
     7201 gcggcaggcc ccgatcaaca tcagaaaccg caggtagcgc cgcgttgcca cggtcaccag
     7261 cttgcgtccc agccgtttgc tcatcacacg cggcagcaac gcgcgcatcg gaaaggccag
     7321 cagcgtgaca agcaggctga tcaggcccaa cgccaggtaa cccaggtgca gcctggcggt
     7381 ttcatgcagg gcacggcggg acgacgacag cgccagcggc tcgttcatga gggcattcga
     7441 ctcgtgtttc atggcatccg tgtctaacgg cggtcagcgc gccgcaggcg ccgcgtcctc
     7501 gaccacctgg atattggcct tgcgcatgcg gatcgcctgc aaggtgcgtt tgtagttcag
     7561 ggccgaggaa atatagaaaa tcgccttgag caccgccagc gaaccccaga tcggcgtctt
     7621 gccgaaaatg tcgcccgcca gcatggacag cagggcctcc ttcacgcgcc acacattgcg
     7681 cggccccatg aacatgtcgc gcatggtcgg gttcgtcacg cggtagatga accacgaaaa
     7741 ctctttcggc cccttgcgca cctggcggtc gaaatgcgcc agcgcggcag cagctttcgg
     7801 cggttcgcgc agacaggtgt caaccgtgtc ggcagcgaca aaaccgccct gcatggccag
     7861 catgacaccc gacgaaaaca ccgggtcgat gaaggtgaac gcgtcaccga tcatcacgta
     7921 gttggggcca tacgtgcgtt cgcaggtgta ggaaaagttg ccggtcgcct cgacatcgga
     7981 cgacagcgtg gcgtgggcca ggcgctcctc gagcgccgga cacagggcga tcgtatcgag
     8041 gaaaaattcc ttcacgggtt tgctgcggct cttgaggtaa tacggccaga ccaccgcacc
     8101 tatgctggtg gcgccgtcgg ccagtggaat gaaccagaac cagccgtggt cgaaccagaa
     8161 aatcgtgatg ttgccttcgt cctgccccgg atgccgcgtg gcgccggtga agtgcgcata
     8221 cagggccgag ctgttgtgtt tggggttgcg gcgcttgatg tcgaactgct tgcccagcag
     8281 ggtgtcgcgg cccgaggcgt ccaccacaaa gcgggcgcgc cagtcctgca ggcggccgtc
     8341 ctcatgctgc gcatgcaccg tggcgccgct gcggtcggcc gcgaaagcca cctcgcgcac
     8401 ccggcagcct tcaatcacct cggcgccggc tttggcggca ttgcgtatca ggatctcgtc
     8461 aagctctgag cgccgcacct ggtaggaaaa cggcatggac ttgtcccagg cgtcgccgaa
     8521 catgaaggtc tggctcttgt tgtcgtgcca gggcgacaca aactcggcgc cccatttctc
     8581 gatgccgatg gcccggacgg cgtccgccac cccgagtttt tcaaacagcg ggaggttggc
     8641 cggcagcagc gactcgccaa tgtgaaagcg cggatggtgt tctttctcca gcaccaccac
     8701 cttgtagccc cgctgggtca gcagcgctcc cgccgtggaa cccgccggac cgccgccaat
     8761 gaccagcaca tcgcactcgc ccggggcggc gggagccggg gcggctggtg aattcagctc
     8821 ggtcatggtt tttccttcgt gggcgttgtc gagcttccag caagccctgc cagagcgacc
     8881 caaaaatggg ctgatcgcgg aggtcaggtt tgcagtccct gagtctacgt cggggcaagc
     8941 gccctgtttg tagtgaaatg tctgatcttt cgggatttac aagcccggcg caatggcaaa
     9001 cgccctccgg gaaaggtcgg aaaggtccgc ctcgcggcga tttccgacat cccggatccg
     9061 gccaccgccg gcgccggaaa acacacgccc atgatgaccc agtccctggg cctgaaggtg
     9121 gactaccccg acacccatgc ggctgtaccg catgggcgac ttttacgaat tgtttgtttc
     9181 aggcagacgc cgaaaaggcg ctcgcctgct cgacatcacg acggtggaca acgttcgaca
     9241 aagcaatgct gcgccgcacc ggcgtttggc catgtcctga ctagaaagtc gctggcgtat
     9301 tcacccaaaa aattcttgca acgaccttgc caacattgcg atacccgtga ggcgtctggc
     9361 tggaaaaata aaacgcgtcc cccgcctgga tcaggtgccg ctcgctcccc agtaccagct
     9421 caagcgtgcc ctccagaaca aagccaacct cttccccgac atgttctatc aagccgtcgc
     9481 tcgcctcgcc tggcgcaacg atgtgaatat tggcctgcaa cagccccccc cgcttgggaa
     9541 gcgtgatgcg ctccagggaa attccaccgg ctctgatcac ggggcgctcg ccattgggcc
     9601 ggactggccc ctccccgggc gcttcttccg tcgtcaacac ggcgatgttt gtgtcgagcg
     9661 tgaccgcaat tcgatgcagc atggccagcg aaggagaggc ctgcccgttt tctatttttg
     9721 agatcaggct ttccgaacat tgcgatttct gagccacctg cgtcatggtc aaaccggcaa
     9781 ccaggcgcgc atgcctcaac cggacgccca gcatggaggg gggtttgacg acactgtcgt
     9841 ccggtttcgg cacagcaaca ggatttgatt tcatgagcat ttccacttgt tttcaccgga
     9901 ggtcaggcat tggccagcga tgtcactgca taagccagcg cctgaacgcc cagaaacaac
     9961 tcctgaggtg cggtttcctc cgcagcatta tgactgaccc cccccttgct cggaacaaaa
    10021 atcatcgccg tcgggcaatg ctccgcgagg tacatggcgt catgaaaggc gccggacgtc
    10081 agccgcaacg gcgcagccag cgaagcgttg ctgcaagcgc gcgtgcaggc ctgctcaatc
    10141 agttccagca tcgcggcagg gaaatgcgtc gggggacgtg aaaaaaggga ttcgaattca
    10201 atctctcctg cccaggaata ggcttgggtg atctccttca aggccacctc gaactgaacc
    10261 aacacctgct catcgacaca gcgaacatcg accgtgaact ccacggcacc gggaatcgtg
    10321 ttgatggaat tggggctcac ctgccatcgg cccagcgtca gccgcaattt acccccaacc
    10381 gccaccgcct gataggcata cagctgctgc gccagcccga cggcagcggc catggcatcg
    10441 gcgcgctcgc ccatgggcat ggtgcccgca tggccggcag cgccagtcac caggacgcga
    10501 taccagcgta cgctctggat gcctgtgacg acgcccagcg gcacaagggc acgctcgagc
    10561 acggggcctt gctcgatatg cagttccacg caagcagaca tcggttcagc cattggccga
    10621 cggggaacat ccggtacagc ccgcagggcc aggtccaacg cctccccaaa actcacaccc
    10681 tgggcgtcga cagattttcg ataggtgggc agacaactcg ggtcgacaaa agcactggag
    10741 cccatggcac ctggaccaaa gcggcacccc tcttcgttgg tccacgcaac gacctcgaca
    10801 gcccgcaggg tctcgatgcc cgcatcgttc aacgcagcaa tcacttccag gcctgcaagc
    10861 acgccatagg ccccgtccag cttgccgccg acgggctggg tatcggcatg gcttccggtc
    10921 agaaccggcg gcaaatcgga tgttcccgga cgacgaaaaa acaggttggc gcagtcatcg
    10981 atcattaccg agcacccgcg cgaacgggcc agatcgatca aataacggcg cgcctggaga
    11041 tcactgtcgg tcagggtctc gcgagaaaca ccgccgtccg accgcccgcc aaaggccgca
    11101 agctcggcaa tgaccgattg caggcgatca tgttgcacat ggtcctgcgc gcgctcgcag
    11161 gccgtcgtct ggtctcgttg ttccggcttg gtcacattca actctttgtc catgttcaac
    11221 ccagactgag ctccagcgta tgcgctgcgc tcaatgtcag cgcatccgcc catcgcgggc
    11281 caatgaccgt gacgcctacc ggcatgccgt gcgctcccgt ccctgcaggc acatgcacac
    11341 aggggttccc caacagcgtc cagagacggt tgaagatggg attacccgtg gcgcccaacc
    11401 ctgcaggcgc ctcgccttcg gcgctggggg caagaagcac atcgaccgac tcgaagacgg
    11461 cgtccagcgc ccgtcgcgca gccatcgcca gcaactgcgc ggcagcgaag tcggccccgt
    11521 tcagcgactg tcccaatgcc aggagttgcg caaaggccgg gctgaacttt tccgcttgcc
    11581 gctggtgctc cggcgcaaac gctgctgcgg cttcataagc catgatctga atttgcacct
    11641 gcgtcaacgc atcgcacgaa gccggcaaca caaggtcatg gacatgtgca cccgcctttt
    11701 caaactggcg tgccgaatgt tcaagcacac ggcgtgcatc gtccgaggcg agatcccaat
    11761 ggggggtatg gcaaatgccc acgcgaaggg ccgattgccg ctgcggatcc aggttgaccc
    11821 gggtcaatgc gccaacaaaa aacgcggcat catccaccga gcgtgcgaac gcgccgaccg
    11881 tgtcgagact gggcgcgagc ggcttgacac cggccagcgg caatgtccca tgggtgggct
    11941 tgtagccaat cacgccacag taggccgcgg gcctcacgat ggaaccggcc gtctgggtcc
    12001 cgaatgccag tggcaccatg ttcgcagcca ccgccgcggc ggaaccgctg gaagatccgc
    12061 ccggcgtgcg gggcgcgtct gccggcgcgc ggggattgca tgtcacgcca ggctgaaagg
    12121 ttgcgaactc ggtggtgacg gtcttgccca gcaccaccat cccggcatcc cggcaggcgg
    12181 ccacgcatgc ggcatcgaac ttgggacgat gccccgcata aataggcgaa ccataggcgg
    12241 ttggcatgtc cacggtatcc atcaggtctt tcaccccgac cgggacacct cgcagcaggg
    12301 cactgctgct atggccgtgg cggtccagtg cacacgcact ttgcagtgcc atgtctgcat
    12361 caaagaactg ccatgccttg accgatggtt cgcaagactg aatctgttcg atgtaggccc
    12421 gcgtcacggc ttcggccgtc aattccctgc gcgaaaccct ggcggccagg tcccgtgcag
    12481 acagcgtcag aatgtcatgc atgtgacctg ctcagccgaa ataggcagaa cgcacttcgt
    12541 cattctcgga cagcgcctgc gccgtacccg aggcaacgac cttgccctgc gaaagaacat
    12601 atccatggtg cgcgatcgcc agcgtttgac gggcgttctg ctccaccaga agaaccgtca
    12661 ccccgagatc attgatctgc tggatcacct tgaagttttc tttcacatac aaaggcgaca
    12721 aacccagcga tggttcatca atcagcagca acttgggttc ggccatcagg ccgcgaccaa
    12781 tcgacaccat ggcctgctcg ccgcccgaca tggtgccggc aagctgcacc gaccgttcct
    12841 tcagacgcgg gaatatctcg tagacccgcg ccagccgctc ccgaatccgg gcaggggagg
    12901 attccagaaa agcgccgagt gccaggttct ccccgacggt catgcgcgga aagaccttgc
    12961 ggccttcggg aatgcaggca atgccgctgg caatgatttt gtgcgttttt tcacccgcca
    13021 ggttgcgccc atcccaccag atttctccct gacggggagg cgtgaggccg agaatcgaac
    13081 ggatcagcgt gctctttcct gccccgtttg accccaggat gcaggtgatc ttgcccggct
    13141 ccacctcgat cgacacatca tgcaggacat tgaccttgtc atagccggtg gtgagattct
    13201 tgacactgag cttgctcatg cagcttctcc gaggtaagcg gtctgcacat gcggatcggc
    13261 cgtgatctcg gcataacgtc cttcggcgat tttcttgccg tag
//
LOCUS       DAIDFG010000050        13196 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_50_length_13196_cov_17.943459, whole genome shotgun sequence.
ACCESSION   DAIDFG010000050 DAIDFG010000000
VERSION     DAIDFG010000050.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 13196)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13196)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13196
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50_length_13196_cov_17.943459"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2197 a         4237 c         4357 g         2405 t
ORIGIN      
        1 ttggacaggc gacagaggcg aagcgtcccg cgagccgcgg cctgcaaggc ctcggacgct
       61 acacgcagta agcaaccgtg ggggctttat ctgtcgtcgt aactgatgga cacgctgtcg
      121 ctcaggggcc gcgcctggca gctcagcaca aaaccttttt gcacttccca gtcttccagc
      181 gtgaagtttt tttccatggc gacctgtccc tcgagcacct tggcgcggca ggtgcagcag
      241 acgccgccct tgcagctgta gggcaggtcc aggccggcgg ccaaggccac gtccagcaca
      301 cgttcgtgcg cgtgcatgcg caactcatgc gccttgccgt cgagctgcac cgacaggcgg
      361 atggcgccaa tggccccttc gtttttcaag cctttttggc cttcagcccc cgtccctttt
      421 gcgtcagcag ctcctgattt catagcgttg gcgggttgcg ccgcgctggt gaaacgctcg
      481 gcgtggatgc gttccttcgg cacgccggcc gccagcaggg cctgcaggca gccttcgatc
      541 atggcttccg gaccgcagac aaaggtctcg tcgatgctgg ccggcggaat cagcgtggcc
      601 agcagttcgc tgaccttggc cgcgtcgagc cggccgttga acagcggcac atcctgcgac
      661 tggcgcgaga gcaggtggac caggctcagg cgggacggat aacggtcctt gagatcctgc
      721 agcgcttcat tgaacaggat gctgccggtg cgctggttgc agtacaccag cgtgaaggtg
      781 gcctgcggct cgccggccag cgtgctggcg atgatggaca gcagcggcgt gatgcccgag
      841 ccggcggccc aaccgacgcg gtgcaccgca ccgctcaggc gcggcgtgaa gcggccgtcc
      901 ggcggcatca cgtccagggt gtcgccgggc tgcagctgcg tggcccagtt ggagaagacc
      961 ccgccttcga ccggtttgat gccgacctcg atgtcgtgtt ctgcggccag gcgctgggtg
     1021 ctgctgcaga tggagtagct gcggcgcacc ggctcgccgt ctatggtggc cttgagcgtg
     1081 aggaactgac ccggcgtaaa gccatacagg ctacgcagct cgggcggaat cgcaaagctg
     1141 acggccacgg ccccggccgc ttcgggggtg acgcgtgtga cgaccagcgg gtgaaagtgc
     1201 ggaacagaca tgggcggctc aatcaataag gcttgaaata gtcgaagggt tccaggcagt
     1261 ccaggcactt gtacagcgcc ttgcatgcgg tggcgccgaa atgcgaggtc tcagtggtgt
     1321 tgaccgagcc gcattgcggg caggccacgg cctcagccgc ctggtgccgg ggcgcaaagc
     1381 ggatgacctt gtcgctgccg ggcgtggtgg tgtgcggcgg cgcaatgccg tagctgcgca
     1441 gtttggtttt ggcggctgcg gtcatccagt cggtggtcca ggcgggtgcg agctgggtgg
     1501 tgatgcgtgc gctgagctgg tgctgcgcca gcgcggcgcg aatgtcgtcc tcgatctgcc
     1561 ccatggccgg acagccgctg taggtcggtg tgatgatgac ttccacgccg ccctcgacat
     1621 ggcgcacgtc gcgcaggatg cccagttcgc ggatggacac caccgggatc tccgggtcgg
     1681 gcacggcttc aagcacgtcc cagacctgct gcagggtgtt caccaccacc ttcccgcttg
     1741 cgggaggtgt tccataacag ctctccggag ctcgtggtgg gtgcgaagcg ctcaccatag
     1801 agcgttcggg tgagcgcgcg ccaggctttg catctcggcc agcagcggcg ccaggtggtc
     1861 gctgtgctgt ccgtttttac cctgcggcag accggtgccg ggcgccgggc gccgcagccc
     1921 ggcttcctgc agggcgtcat tgacgaggtc gtcccattcg cttttcagtg cggcaacatc
     1981 gatgggtcgg ccttccgtat ctgctactgt ttcaatagct gatggcgccc agtactcctg
     2041 ggtgtaaggc attaaatgct ccaaggcggc ctgcgtgcgg gcgtgcgatt cggtggtgcc
     2101 gtcgcccagg cgtaccagcc agtcgcgcga gtggcgaaag tggtaacgca cttccttgaa
     2161 tgacttgccc gcaatcgcgg ccagctgcgc gtccttggat ttctgcaagg cttcccacag
     2221 cagcagcatc agcgcgctgt aaaaaaaatt gcggacgatg gtcaccgcgt agtcgcgctc
     2281 cgaagcatcg gaggattgcg gcagctcgag caacgtgtag ttccggaact cgtcagcgcc
     2341 gcggaagtag gcgagtgaat cctcagtagc gccactgccg gccaccgtgg ccgcatgctg
     2401 gtacaacaaa cgcgcctgcc cgatcaggtc caggctcatg ttggccagcg cgatgtcttc
     2461 ctccagcacc gggccgttgc cgcaccactc ggcgttgcgc tggccgagca ccagcgcgtt
     2521 gtcggccagg tgcagcaggt agcccagcgg ggctgttctg acgggggctt caacggtatt
     2581 cacatgtggc ccacttcgtc agggatctcg taaaaggtcg ggtgccggta gatcttgtcg
     2641 gccatgggct cgaaaaacgc ggccttgtcg tcgggttcgc tggctgtgat ggtgttggac
     2701 ggcaccaccc agatgctcac gccctcctgg cgccgtgtgt aaacgtcgcg cgccatctgc
     2761 agcgcctgct gtgcatcggc ggcgtgcagg ctgccgcagt gtttgtgttc cagcccttgt
     2821 ttgctgcgta cgaacacttc ccacagaggc cagtcgttgt gtgtgttgct catgctgctt
     2881 ccttcatctg gcgggctttc tgtttggctg catgggcctg cgccgcatcg cgcacccact
     2941 ggccgtcctc gtgggctttc acgcggttgg ccaggcgctc cttgttgcag gggccgttgc
     3001 cgttgacgac ctcccagaac tcgtcccagt cgatcgcgcc gtagtcgtgc tgccggcgct
     3061 cctcgttcca cttcaggtcg gggtcgggca aggtcacgcc cagcaccgcg gcctgcggca
     3121 ccgtggcgtc gacaaacttc tggcgcaggt catcgttgga aatgcgcttg atgccccagc
     3181 gcatgccttg cgcggtgttg gcgctggccg catcgggcgg accgaacatg gccagcaccg
     3241 gccaccacca gcggttcact gcgtcctgca ccatggcgcg ctgctcgggc gtgccctgca
     3301 tctgcaccat caggctttca aagccctggc gctggtgaaa actttcttcc ttgcagatgc
     3361 ggaccatggc gcgcgcatag ggtccgtagg agcagcggca gatcggcacc tggttcatga
     3421 tggccgcgcc gtcgaccagc cagccgatca cgccgacatc ggcccactgc agcgtcgggt
     3481 agttgaagat ggagctgtac ttggccttgc cggtgtgcaa tgcatcgagc atcgtgtcgc
     3541 ggtccacgcc cagggtttcg gcggccgaat acaggtagag gccgtggccg ccttcgtcct
     3601 gcaccttggc cagcaggatg gccttgcgtt tgagtgaggg cgcacgcgaa atccagttgc
     3661 cttcgggctg catgccgatg atctcgctgt gcgcatgctg gctgatctgg cgcaccaagg
     3721 tgcggcggta ggcctcgggc atccagtcct gcggctcgat cttctcatcg gcgtcgattt
     3781 tttcgtcgaa gcgtgtctgc aacgcgtcgg ggggcgccgg gatcgaagcc agggccgggg
     3841 cggacgtgtc gggcacgtcg agtgattgtg tgtacatgtc ttgtctcctg tcacccggtc
     3901 tgcagggcgg tggtaagccg gagtatagcg aattaaccga ccgatcggtc ggttaattgt
     3961 tgactccgct gcagggggct ttcagctcac ccgggcgagg tccgccaaca gggcgtcggc
     4021 cgatgcataa cggttgtcgg ggtttttcgc catcagtctg ttgacgatgg gctgcagggc
     4081 ggcgtgcgcc gcaggcagca ccggcgtgtc cgcatgcagg tgcctggcca gcagcagttc
     4141 cagcgattcc gcgatgaaag ggcgccgtcc ggccagcatc tcgaacagca tgacgcccag
     4201 gctgtacagg tcggagcggg ccgtcacggc tttgccgctg acctgctcgg ggctcatgta
     4261 atagggcgtg ccgaccacat caccgtgccg ggtctgcgtc aggcccaggc tttcggcctg
     4321 cagcgtggat ttggcgatgc cgaaatcggc cagcgccacc gagccgtcgt tgcgcagcat
     4381 gacgttttcc ggcttgatgt cgcggtgcac gatgccgtgg ctgtggatgg ccgccagggc
     4441 cagcgccagt tgttgcacca ccgcaagtgc ctgctcgcgg gtcatgccgg ggccgaacag
     4501 gccgcgcagg tctccgcgct cgaaatactc catcgcgatg taggcgtgtt cgtcgctgaa
     4561 gccctgatcg tggatgcgga tcacattcgg atgcttgatc tggctcagca gcgcgtactc
     4621 ctggataaac cggaccaggt gctccgacgc ttccttgccg cgcgaatcca gcaccttgag
     4681 cacgatgggc aggccgtcgg cttcgcgttc ggccaggtaa acctcggtca tgccgccggc
     4741 gccgattttc cgggtgacgc gatagccctt gaggcgcacc ggctcggtgt cgggcgcaaa
     4801 gcgctgctct ttcagcgcgg ccagcttggc ctgcagggca tccttgacgg cccgcgcggt
     4861 gatctcggag ttggtcctga ccgccgcgct gatctcgttg gcacgctcgg tcaggctggc
     4921 catgccgtgg atcttgtcgt ccatcccggt caccaggccg gtgatttcca ccacgtccag
     4981 tgtgaggttc ttgccgggaa cggtcagtgt ctcctgcgcg ccggtctgga tgccggggcc
     5041 ggcaccggcc agcacggcgc ggctggccag cacggtccag ccgcgctcgc gcgtgaccgc
     5101 ctccaggcgc gcggcaaggc tgacggtatc gcccaccggc atcagctctt ggctgcccgg
     5161 cgggcccatg cggcacagcg ttgtctcgcc gctgtgcagc ccgatgccga tggcaaacgg
     5221 cggcaggccg cggtccggga aacgctgtgc cagccagtcg cggaactcgt gggctgccag
     5281 cgccatcgcc agcgccgcgg aaacggcgcg gcgctgcgcc ggcagcggcg agccgctgag
     5341 cgtgtcggca aacaccgcca tcaggccgtc gccgacgaac ttgaggtgcc gcccgccatt
     5401 cttgagtacc ggctggcaac tgcgatcgaa gtagccggtc agcaattccg aaacctcacc
     5461 agcctcgagt ttttcggcca gcgaggtgaa gtggcgaatc gtcgacacca gcaccgtggc
     5521 gtcgagtgcc tgcccgtctt cgctgtccgc cggcatgtcc accggaaagg cttcaggccg
     5581 gccgccgcca atgtcgctgc cgaaagccgt gcgcaggcgc gcctcgagtg ccgccaggct
     5641 ggacgcaatg cgtgtgtcga cgagaccttt tttgcgcagc tgcgcgttga cggcttcgag
     5701 cagctcggtg cgcgtgaaag gcttggacag gtagtcgtcg gcgccgccgg tcatgccgcg
     5761 gcgcatgctg ccgcggtcgt ccagcgcggt cagcagcatg aacggagtgg tcgccagcgc
     5821 cgggtcggcc cgtaccgcag ccagcagctc aaagccatcc atgccgggca tgttgacgtc
     5881 cgaaatgatc aggtccggcg tgcgggccct ggcgtgtgcg aggccggacc ggccatcggc
     5941 ggccgagacc gcctcgaacc cctccagcgt cagcaggcgg gcgatgttgc tgcgaatggc
     6001 gtcgtcgtcc tcaacaacca ggatcacatg catggacaga ccctttgccg gcggcagttt
     6061 ccggtcagcc gccgtggtcc agcgtgaagc cggcgttgcg gtcctgtccc aggcgcgcca
     6121 ccagctgcgg cagggccgcc tgcaggtcgg ccttgagcgt gtgcggcggg ttgatcacaa
     6181 acatgccgct ggccggcagg cccgggcgtt tgatgtcgcc ggcctcgtcg gtggtcagct
     6241 tgctggactt gaccgtgagc gtggcgttga gccagctgcg gcccgccttg gtcgccagcg
     6301 tcttgagctt gcgcggcagg tcatgggcct cggggcgcgg aatgatgggg taccagacca
     6361 cataggtgcc ggtggcaaaa cgcttgacgg catcgcccat gcaggccgcg acgcgggcgt
     6421 agtcgctctt gatctcgtag ctcggatcgc acagcaccat ggcacggcgc gccggcggcg
     6481 gcaaaaaggt cttcagcacc tcaaagccgt cctcgcacgc caccgtgacc tggcggccca
     6541 cgcccagttg cgcgatgttg ccggtcagcg acttgaaatc ggtcgggtgc agttcaaaca
     6601 gcttgagctt gtcgcggccg ctgaggaatt gctgctcgat gaacggcgag ccggggtaaa
     6661 tcttcaggtg cgccggatca ccgcttgcgg cgaactgcgg gttcaggccg cgcagcaggt
     6721 cgatatagtc ctgcagcgcg ggggccaggg gttcgtcggc cgctgctttt gctgcgcctg
     6781 gcgttttgga ggtcgctact tttttggtag ctgctggcgc tgatgcagaa agggttgtgg
     6841 gcgcttttga tgcagagacc aggcgaaaaa tgccctcctg cgcttcgccg ctggtttcgg
     6901 tgtagtcgcc gtcgaggcgg tacagcccgg cgccggcgtg cgtgtcgatc acggtcagcg
     6961 ccgtgtcttt ctgggtcaaa tacttcatca acgcaatcag cgtggtgtgc ttgagcacat
     7021 cggcatggtt gccggcatgg aaggcgtggc gataactgaa catgtcgcta tgttaacggg
     7081 cgccgccaga caggctcttt ttttgaccgc gccgggcggc tgggccggct gcttgcaatc
     7141 tgcaacacaa taccgacgga cgcagcattc tggccgcccg ggcgccttga cggcggcagg
     7201 gcgcccatgc ctaatgacag ccccacgttg ccatctacag ctctcctgtt gccacagtcc
     7261 cttcatgaca gcctcgccca gcccgacccc cgtttccctg atgacgcatg tggatgcctt
     7321 gccggtgggc acgcgcctgg ccgagtttga agtgctgggt ctgctgggcg tcggtggttt
     7381 tggcatggtc taccaggcgt tcgaccactc gctgcagcgg ccggtggcca tcaaggagtt
     7441 catgccggcc acgctggcct gccgcggcgc ggatggtgcg gtggtagttc gcgacccggc
     7501 cgaccaggcg gcctttcagg tcgggctgca gtcttttgtc gccgaggcgc ggctgctggc
     7561 gcagttcgac catccgtcgc tggtcaaggt tttccgttac tgggaagcgc accagacggc
     7621 ttacatggtg atgcccctct accgcggcat cacgctgcag gcggcgcgca gccagatgcg
     7681 cagcccgcct accgaggcct ggctgcgcaa ggtgctgtgg gcggtgctgg gcgcactcaa
     7741 gaccctgcac cagggccgcg cggtgcaccg cgacgtctcg cccgacaaca tcttcctgca
     7801 ggacatcggc ccgccagtgc tgctggacct gggcgcggcg cgccatgcca tgggcggcaa
     7861 gacgcaggac ctgaccgacg ccttcaagct gagctacgcg ccgctggagc aacacgccgg
     7921 cgcgcccgac atgcgccagg ggccgtggac cgacctgtat gcgctggccg ccgtggtgca
     7981 tggcctgctt tgcaacaagc caccgctgcc ggccactttc cgggccgtgc gcgaccgcct
     8041 gccgcctttt gcccgtgtgg cgcgcacggt gcaggagcag tttggcctcg gttattcgca
     8101 gccgtttgtg caggcgatca gccaggcgct ggcgatcgac gccagggaca ggccgcaaag
     8161 cgtgggggcc ctggtgcaac tcatggcgct cgaaacgccc aatggcatgt cccgtttcga
     8221 ctggcgtgcc gaactgggcc cgatctgggc accgccgggc ctgtccgtct cacggctggc
     8281 ggccagggcg gaaccacctt cgccgcaacc tcctgcaccg gcaccccgca atgacccgac
     8341 acaacccctg gcggctgcgc cccggcggcg ggagcttgcc gcccagcggc cggctgtttc
     8401 ggcaccagcg ccggccgccg ccgtggccgc gcccgcccgg tctggccttt cgtggcgtgt
     8461 gggtgctggc gtggtggccg tggccatcgt tgccctgttg ctgggactga tgctgggacg
     8521 gggggcgcgc gcgcccgaaa cccggctcgc gcaggctgcc ccggccgctg ctgagatcgt
     8581 gcagcgggtg gtcagcccgc cggccgccac attggccgaa cccgtgccgg tgtcggtcgc
     8641 acccgtgcag caggtcgcag cggccgcccg ggcacctatg tcgccagagc tgcggccaag
     8701 ccctgtcgtc aagcgcgcca cgccgaaaaa gacagcgacg ccggccgatg ccacgagccc
     8761 ggcgcccgtg ccgccgccgt tggttgccgt gacaccggag cgggtggagc tgccacagcg
     8821 tgtgaagccc cccgatccgc tcgccttgtg tgccgacagc aacttcgtga cccgacccat
     8881 gtgtattttt cggcagtgcc agaagccgga gatggccaag ctccccctgt gtgttgaaaa
     8941 caacgcccga ctgcagaaca gccggcgggc cgacgggttt tagctcaaaa aggccccgga
     9001 attggccgcc gaacggccag aacagggcgt tccggccgcg cggacccgcg aagctgccca
     9061 cgcagccccg gattttgtat aatggaaggc ttcgcagcga tcggctggcc ccgcggcgaa
     9121 gcttgaaccc cacctaagag gtttccatca gagaaacacc gaccgtggaa aagggctgct
     9181 caaaccaact tttgaaaagt aaaccatgac aactttcagc gcaaaacccg ctgacgtggt
     9241 gcatgagtgg tttgtgattg acgcgaccga caaggtcctc ggacgagtag ccagcgaagt
     9301 tgctctccgt ttgcgcggca aacacaaggc catttatacg cctcacgtcg ataccggtga
     9361 cttcatcgtc atcatcaacg cagcccagct tcgtgtcacc ggcgccaagt cgaccgacaa
     9421 gatctactat cgccattcgg gttaccccgg cggtatctcg gctacgaatt tccgcgacat
     9481 gcaaaccaag ttcccgggcc gcgccctgga aaaagccgtc aagggcatgc tgcccaaggg
     9541 cccgcttggt tacgccatga tcaaaaaact caaggtgtac ggtggtgcag agcatccgca
     9601 taccgcccaa cagcctaaag tgctggaaat ttaaggagcc ttgagatgat tggtgaatgg
     9661 aacaatggca ccggccgtcg caaatcaagc gtcgcccgtg tgttcatcaa aaaaggcact
     9721 ggcaagatca cggtcaacga ccgtgacatc cagactttct tcggtcgcga aacctcgatc
     9781 atgatctgca agcagcccct gtacctgacc aaccatgtcg aaaccttcga catcatggtc
     9841 aacgtgcacg gcggcggcga gtccggccag gccggtgcga tccgccacgg cattacccgc
     9901 gcgctgatcg actatgacac caccctcaag ccggcgctga gccaggccgg gtttgtcacg
     9961 cgtgatgccc gtgaagtcga gcgtaagaaa gtcggcttcc gttcggcacg ccgtcgcaag
    10021 cagttcagca agcgttaatc cgctgttgct gtctccgaaa agccgcctgg tttgcgcctg
    10081 gcggcttttt tcatgggtgt tcgcagccag tgccgggcct gccgatacaa atccttgccg
    10141 ctgcctgccg ggtagcactc agcgcaggct gcatctgagt gcacaattca cttcctggaa
    10201 gacaacccaa ggtcgctttt gaaattccgc ctgtttcgcc gccgcctgac catcagctcc
    10261 ccgcgcatgg ccattcgcac gtccttgccc tggccgctgc gctgggcgct gggcgcgatg
    10321 atgctggggt tttctgcggc catcgcgctg tggaccttcg aactcggcgt cagcatcgcg
    10381 gggctggaca aggatgccaa gctggagctg accaggctgc gggaggaggt cgccgcactg
    10441 cgcgcggagc gcgacaaatc gcagtcgatc gccaacattt cgggcagcct gctgactgcc
    10501 gagaaggccg cgcaggaaaa gatggtggcc cagatccgcc agctcgaagc ggacaaccgg
    10561 ctgctgcgcg acgatctcgg tttttttgaa aaactcctgc ctgccggcgg cgtggaaaac
    10621 gccgccattc gcggcctgca ggccgaagcc ctgaccgaca cgcaggtcaa gtggcaagtg
    10681 ctggtgatcc agccggtcaa ggacgcaccg gtgttcaacg gcaagctgga actcactttc
    10741 agcggtaccc tggccggcaa gccatggaca acaggcttgc cgggcggcgc ccagctcctg
    10801 cagttcaagc agtaccgccg catcgaaggg gtgctcgacc tgccccccct ggccgtggta
    10861 caaacggtga ccgccaaggt cctcgaagga acggccgtcc gctccgtcca gaccatcaag
    10921 ctctgaaccc ccgcttcaac atccagaatc tcacaggatt tgtccatgtt tggtaaaaaa
    10981 tcgcagcccc cgatcaaaag cctgattgcc cagggcagtt gcatagaagg caacctgaag
    11041 ttcaccgacg gcttgcgcat cgacggcgaa gtgatcggtg acatccgtgc caacgagggc
    11101 agccccagca ttctggtgat cagcgagtcc gcctgtgtca ccggggccat ccatgccgac
    11161 cacgtcatca tcaatggtcg cgtcatggga ccggtgcatg cggcggaact cctcgagctg
    11221 cagcccaagg ccaagatcca gggcgatgtg gcttacaagg cacttgaaat gcaccagggc
    11281 gccgtcattt ttggacagtt gcggcccacc aaccacgaag tcagcgacaa acccctgctg
    11341 aagctggccg ccaataacgt tgagaaagcc cgggaagaag ccccggtcag tggctacggc
    11401 cgactctaag ccagcgcggg ccttatggtt gattgaattg ctgtactatt aaaaacataa
    11461 gttctgaaaa acggagttcc ccatgagtgc agtagctgaa aacatccaga ccgaaatgcc
    11521 cgcccccttt gtctttaccg acagcgcagc ggccaaggtg gcggacctga ttgccgaaga
    11581 gggcaatccg gacctgaaac tgcgcgtgtt tgtgcagggc ggtggctgct ccggcttcca
    11641 gtacggtttc accttcgacg aaatcaccaa tgaagacgac accaccatga ccaagaatgg
    11701 tgtgtccctg ctgatcgatg ccatgagtta ccagtacttg gtgggcgctg aaattgatta
    11761 caaggacgac cttgaaggcg cccagttcgt gatcaagaac cccaacgcca ccagcacttg
    11821 cggctgtggc tccagcttct cggcctgagc tttcccggcc tcaaaacaaa ggacctacgg
    11881 gtcctttttt catgcccgcc tctcaatgtc agcagtcgac accgtggggg ccaaataccg
    11941 ggcgcccagc acgcgcgggc cgcgggcgcc cgtgacggct ggcaaactac ctggctgccg
    12001 gtggtgccag gcccgggcca accatgcaaa agcagcggct tccacctgca gcggcggcag
    12061 gccatgcgtg gtggtgggct ggacgctcac cccgggcaga gccagggcca gttgccgcat
    12121 cagttcgtca ttgaaggcgc cgccgccgca gaccagcagt tcggaggtgc ggggggcgtg
    12181 gcgcatcagg tcgcgcgcgc aggcctgcgc cgtcagggcg gtgagggtgg cctgcacatc
    12241 ctgcgctgcg ctggcctcca ggccgctgag ttgcccggcc agccattcgg ggttgaacag
    12301 gtcgcgccct gtgctttttg gcggcaccct ggaaaagtag ggcgccgcca gcaggcgcgc
    12361 cagcaggccg ggaagcacgg ttcctgacgc ggcccagtcg cccaacgcgt cgtacggctg
    12421 gccggtgtgc tgcaggcacc agaagtccat cagggcgttg ccggggccgc agtcgaaacc
    12481 tttcaccggg ccgccgtgcg ccggcagcag cgtcaggttg ctgatgccgc cgatgttgag
    12541 aaccgcaacg tcccgttcgg gctgcgcaaa aacagcgtgg tgaaacgcag gcaccagcgg
    12601 cgcgccctgg ccgccggccg cgaggtcggc ggtgcggaaa tcggcgacca cgtccagccc
    12661 ggtgagttca gccagcaggg ccgggttgtt cagttgcagt gtgtagcctg cctctgatcc
    12721 gccgctgagc gtgtcgtccc cgaactcctg cggccggtgg cgcacggtct gtccgtgagc
    12781 gcctatggcg tgcacctggt ccgccgtgat gccctggtgg cccgcctgcg ccagcagggc
    12841 atggaccacc tgtgcgtaca gcacggccag ctggttggca gccagcgccg cgcggtgcag
    12901 ttcgttgtgg ccggcggtgt tgagggccag cagttcggcc cggaaggtgg ccgggaaggg
    12961 gacggacgta gaggccatga cacgtggcac cgccggctcg aaatccacca gaacgccgtc
    13021 cgcaccatcg agcgaggtgc ccgacatcaa ccccatgtag taatcagcca tgcgtcattt
    13081 tgccgcaagg gtgactggag gcgcgatttg tggatgggcg accgtgggag ctcttcagac
    13141 cagccagggc cgcgggtccg gctttgccgg cccgcaggcg cagcggcccc ctcggg
//
LOCUS       DAIDFG010000051        13145 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_51_length_13145_cov_17.330634, whole genome shotgun sequence.
ACCESSION   DAIDFG010000051 DAIDFG010000000
VERSION     DAIDFG010000051.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 13145)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13145)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13145
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51_length_13145_cov_17.330634"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2231 a         4288 c         4377 g         2249 t
ORIGIN      
        1 ggccgcccga gcccggctga tcgcgcgccg caggggactt gttcagcgct tccctaaccc
       61 agcccggaag gctgcgtggg ctggaagtcg gcaggggcgc ccgctgccgc ctcctgctcg
      121 atgctggcgt cgcgtgccgc aaacatgttt tgcaggtcct tcaggtcgcg catcacatcg
      181 tcttcggcct catccgccgg cacggccggt gctgctgcca tgacggctgc gggtgctgca
      241 gcggcaagcg cagcgggcgc ggcggcacgt gccggcggcc cccagatggc cgggtcgggc
      301 atgtcgggga aggggcagct gtccccgacc ttgaattccc aggaaatccc atgcagccag
      361 gtcccggact gtggctcgaa ctgggtgatc gcggcgagca gctcgttctg gaaagcgaag
      421 gcataggtca tcaggcgtgc gtcccaatga cgcagcacca gccgcacgta cacgccgggg
      481 ccgcggctgc ggctgttgac caccagcatc tggcattcca cccagtccgc cgcaatgcca
      541 tgcctgcgca ggcagtcgcg cagcagcatc tgcacgagct ggcggcgcgt gccgttgtcg
      601 gagttgtcct cgatggtgtg ctggctgtcc cgggtggttg tcgccggcgt gcgggccgcc
      661 ggtttcagcc cggaatcctg accgtcggcc gtcttgttct tcagccccag caatcgctcg
      721 aacacttgtc tcatgtggtg aacccttcgc ggccggccct ggccagagcg ctctggccgt
      781 tcggtgccgg cttgtcaatt gatggtcgtc caagtgggaa ggcaagtcaa gcatccagcc
      841 tgtgcagcgc ttcttcctcc caccaggcgg ccgcagcgta gtaactttcc cacacgcagc
      901 cgttgcagcc ggggccgcag caggtcgtgg gttcggacgg cggcgtccgc gaggccatgc
      961 ccgcgtcggg gctggcaggg gcggaagatg ttgtcatggg tggggcaaag tcgtgcgcgg
     1021 ccacgtccta cctgaggatc agcgtcgggt gcttatgtga tagcccgtcg ggtgctaaaa
     1081 acagtcatcg cgatgtcaac gcgacttcaa cgcgacctca tcacgggctt ttcgaaggat
     1141 tttcatggac agcagcagcg ccgcctggta cgcccagttg agcaagcctt tttttgcgcc
     1201 accggcaggg gtgtttggcc cggtgtggac ggttctctac gtcatcatcg ccatcagctt
     1261 cgggtttgtg ctgctgcaat atctcaagcg ccgcctgccg tttcgcgtgc tgctgccttt
     1321 catcctgaac ctgctgttca acctggcgta cacgcccatc cagttcggcc tgaaaaacaa
     1381 tgcgctggcc agcctcgaca tcctgctggt gctggccacg ctggtctggg ccatggtggc
     1441 catccggcag cgcgcgcgct gggtggtggg ggtcaacatc ccctacctgg tgtgggtgct
     1501 gtttgcgaca gtgctgcagc tcagcatcac ctggttgaac cggggcgcct gaagaagatg
     1561 cgccagtgca ggctcacagg cccagcaatt ttttggcgtt gcccagggcc ttcatcgatt
     1621 cgtcgtgttt gccggccttg tggaaggtct ctccttcggc acgcagggcc ttgaccttgc
     1681 ccatgtcggc ggcggccagt ttggggttgc tggcgagctt ggcgtctatc gccttcattt
     1741 cattggggca gttgtgggcc agggccagtg acgaggcggt cagggccgcg gcagcaagga
     1801 tgagtttcat gcaatctccc atgtccaatg tgcgtcagaa aatgctgaca gtaaatgcta
     1861 acaatgatcg ccccgtggcg gcaagcgttt gaccgcaagg ccggccgggg cattgccata
     1921 aaatcagcag cttgcgcccg ctgcctgcat gcaccgacct gcggcggccg gctttcccac
     1981 cgactaccgg gttgacccat gtctcttgaa gttaccgaag cccagacggg cgagcagccc
     2041 gtcgccacca tcctgatcat gcatggcctg ggtgccgacg gccgcgattt tgttcccgtg
     2101 gccgagcagc ttgatttgtc cagcgtgggc ccggtgcggt ttctgtttcc ccacgcgccg
     2161 gtgattcccg tcaccatcaa cggcggctac cagatgcccg cctggtacga catcctgggt
     2221 gccaacctga cggcgggcca ggacgaaacc ggcatgcgcc agacccaggc caccatcaac
     2281 acgctgctgg aacgcgaggt ctcgcgcggc attccggcca gccgcatcgt ggtggccggt
     2341 ttttcccagg gctgcgcgat ggcgctgatg acgggcctgc gttatcccga acgcctggcc
     2401 ggcatcatgg gcctgtcggg ttatctgccg ctggcgcata ccgtggcggc agagcgcagc
     2461 ccggccaacg aaggtctgcc gattttcctg gcgcacggca agcgcgacgg cgtggtgccg
     2521 ctggccgcgg ccaccggggc acgcgaagcg ctgacggcat tgggttatcc ggtggaatgg
     2581 cacgactacg acatggaaca ctcggtctgc atggaagaga tcgccgacat gaaccagtgg
     2641 ctgctgcggg tgttgtccta acgtctcgag cgaaatccgg cagccgtaag ggctttcccg
     2701 taagggggcc gcgccgggtt tcaggtccgc cggctgcgga cggctgtatt tttaaacgaa
     2761 atcgtgctct ggcccaatca ggacgggcgc gaccagctat caaaacaata gtaactcgta
     2821 cggaccgaaa acggcaggcc tcgccgggct gcgcggcacg gccaagcgcc aggccgcgcc
     2881 gcctgcaaac aaccccggcc tgtgatacct tgcacgccac gaaagattca gagggaaaac
     2941 tatatgacca gcttgcatgc gcgatctgcc gcgcttctgg gagaccgcct ccaggcgctc
     3001 ggccctgcca ggctgaaagg catgcgtcgc ggcatcgaga aggaaagcct gcgcgcccag
     3061 cccggcggcg cgctggcgct gacgccgcac ccggtcgcgc tgggttcggc cctgacgcac
     3121 ccgtgcatca ccaccgactt cagcgagtcc caggtcgaac tggtcaccgc cgcgcacaag
     3181 ggcctgggcg acggcgcgga cgccgcgctg cgcgaactca cgcagattca ccagttcacc
     3241 taccgcgcga tgcaggcggt tggcgacgaa atgctctggg tttccagcat gccctgcggc
     3301 ctgccgaccg acgagacgat tccgatcgcg cgtttcggct cttccaatgt cgggcgcgcc
     3361 aaaagcgtct accgcatggg cctgtcgcac cgttatggcc ggcgcatgca gaccatctcg
     3421 ggcatccact acaactggtc gctgcccgag gtgaccagcg agcagtactt tgcgctgatc
     3481 cgcaacttcc gccgccatgc gtttttgctg ctttacctgt tcggcgcctc gccggccgtg
     3541 tgttcctctt ttgtcgccgg gcgccagcac gagctgcagc agctctcgga cagcaccatg
     3601 tacatgccgc acggcacctc gctgcgcatg gggcgcctgg gctaccagag cgatgcgcag
     3661 gcctcgctgg ccgtcagcta caacagcctg gaaggttatg gcgcgtcgct gcaggacgca
     3721 ctgacacggc cttacccggc ctatgaggcg gtgggcatcc agaaccccgg cggcgactac
     3781 aaccagctgg gcaccacgct gctgcagatc gaaaacgagt tctacggcac cattcgcccc
     3841 aagcgcgtga ttttcccggg cgaacgcccc ttgcatgccc tgcgcgagcg cggcgtcgaa
     3901 tacatcgaag tgcgcctgat ggacctggac ccgttcgagc cggtcggcat caacgcccag
     3961 accatgcgtt ttctcgatgt gtttttgctg cactgcctgc tcagcgacag cccgccggac
     4021 acgccgcagg aaatcgccga actcaagcac aaccagcacc acacggcggc gcgcgggcgc
     4081 cagagcggcc tgctgctcaa gcgcgccggg cgcgaggtac ctttgaccgg ctggggtgcc
     4141 gaggtggtgg cgcagtgcgt gccgattgcc gccgcgctgg acgcggcgaa cgggggcacg
     4201 cagtacagcg acaccctgct ggccgccgag gcggcactgc tggaccccgc caccctgccg
     4261 tcggcccgcg tgctggcggc catggcgaag gaccacgaga actcttttgt ggcgtttgcg
     4321 cgttcccgct cgaccctgac caaagaggcg ctgctcggcc tgccattttc cgcagagcag
     4381 caggccgcgt tcgaggcgct gtcgcgccag tccgtcgccg agcagaaaaa gatagaagcg
     4441 gccgacacca tgcctttcga gatctaccgc cagcagtatg tctctgccga ccggctgggc
     4501 ttgccccgcc gcgaggccgc tgccgccgtg taagcggcgg cttttcttgc gcgcaggccc
     4561 ctcggggctc aggccggcag cagggccagt tgacgcagca cttcatgggc cgccgcttcg
     4621 gacgaggccg ggttctgccc ggtgatcagg ttggcgtcgt tcaccacatg ggactgccag
     4681 tcggcgccct tgctgtagtc gccgccgttg ttgcgcagca tgtcttccac caggaagggc
     4741 acgacatcgg tgagctgcgc cgccgcttcc tcggtgttgg tgaaacccgc aacctttttg
     4801 ccctggacca gcggtgcgcc gccggcggcc ttcacgtggc gcaacacacc gggtgcgtgg
     4861 cagaccgcgg ccaccggttt gccggcggcg aacatgcgct cgatcagcac gatcgagtcc
     4921 ctgtcttcgg ccaggtccca cagcgggccg tggccgccgg ggtagaacac ggcgtcaaag
     4981 tccgcctcct tcaggccggc cagcttgacc gtgtgcgcca gcgcctgccg ggcctgggga
     5041 tcttccttga agcggcgtgt ggcgccggtc tgcgcgtcgg cagcgtcgct tttcgggtcc
     5101 agcggcggtt gcccgccctg gggggaggcc agcgtgatgt ccgcgccggc atccttgaag
     5161 acgtagtagg gggctgcaaa ttcttccagc cagaagccgg tttttttgcc ggtgtcgccg
     5221 agctgatcgt gcgatgtcaa aaccatcagt atcttcatgg gtactccttg aaagcgcgga
     5281 cggggctgcg cggtgaaaat ggggtcaagc cgccatggcg cgaccagttc accatcttcc
     5341 cgcgccaggg ccggtcgccg cgtaggcgag gggaaggtct gcctgtcagg ccgggtcgcc
     5401 cgggtgggtg acaatccctg ctggccggct gcatcgagct gcagcgtcct gccaacgaaa
     5461 agaaagaaca ctcagacaat ggccatccag tggttccccg gtcacatgca cctgaccaag
     5521 aaggcgatca gtgagcgcat caaggaaatt gatgtggtca tcgagctgct ggacgcgcgc
     5581 ctgcccggct ccagcgccaa ccccatgctg gccgaattga ccggcgccaa gcccgcgctc
     5641 aaggtgctca acaagcagga cctggccgac ccggcgcgca cgccgctgtg gctcgcacat
     5701 tacaacagcc tgcccggtac cagcgcgatt gcgctcgacg ccagcgagac cacgccggcg
     5761 cgccgcctga tcgacgcctg ccacgcgctg gcgccaacgc gcggcagcat ggtcaagccc
     5821 atgcgggtgc tgatctgcgg cattcccaac gtcggcaagt ccacgctgat caacaccctg
     5881 accggcaaac gcgccaccaa gaccggcgac gaggccggca tcaccaagct cgagcagcgc
     5941 atcgtgctgg ccgacggctt ttacctgtgg gacacgccgg gcatgctctg gccgcgcatc
     6001 atcgtcgcca agagtggcta caacctggcc gccagcggcg ccattggccg caatgccttc
     6061 aacgaggaag aggtggcgct ggaactgctg gactacctga tccaggccta cccggccctg
     6121 ctggaggcgc gttacaagct caagctgacg cctggcatca ccgacgagca actgctcgag
     6181 gagatcggcc gcaagcgcgg tgcggtgttg tccaagggac gcgtgaacct gcagaaagcc
     6241 gcggagatcg tgatctacga gttccgcgcc gccacgctgg gccgcgtcac gctggaaacg
     6301 cccgaggagt tcgcccagtg gctggccgct ggccagctgc ttgatgccga gcggcaggtc
     6361 aagaaggacc gtatagagac cgaccgcctg gtgcgtttca agaaaatccc gcgtcctccg
     6421 cgcgaggact gacgacggcg cgcagcggat cgggcgccgg cctttcagga aggccccggc
     6481 gtggcgactt cggaaagggt atcgccgccc ttgaaggctg cgtagtaaaa cggaattctc
     6541 gacagcgcgt gccgcgccgc ctggtcacgg atgtcattac gcgcgccgtt gaatcgccgg
     6601 gtctcggtga acagcctcgg tggctggccg tcctttcctg aaaagagcca ggcaaaacat
     6661 tgcgtgccgg cgggaatcgc cgggtcgatg ttgtcggtca cgcccgtatt ggccaccgcc
     6721 aggttggcct ggctgctgcg cagtgcgccc tgcgccatct cggcggccac ctgctcgctg
     6781 gtcaggttga agcgcgcaat ggtgtccggg ctgaccccca ggcggcgctg cttggctccg
     6841 ggcgaatagg tgacgcaggc actttccagc agttcacccg cacccggcac gtcggcaagg
     6901 tgggacgcaa tcaggccggc ggtgcatgac tcggcggtgg tcagaagcag tgattgcgcc
     6961 gtcatgaacc gggcgacttc tttcagcggc gtgctggaca tgaaacctcg cgtaagttgc
     7021 agaagatatc ccacgatggc agacatgtga cgggccgtgt gtcgctggaa aaacatgccg
     7081 cctgtaggaa aaaagcgaat cccgacgcgg ctggcggaat tgaaaaatcc cgcagccggg
     7141 ctcttgaaac gcggcgcgcc agccactatg tccctgccgt caggggtcgt ccatgcgtgg
     7201 acgaccgcgg tttcaataac gccggggctg tgccccggcc ggctggcagc ccacctgacc
     7261 tgccgcccct tcatgatttt cacgaaagga gattgtcatc atgaacggat tgaacctgta
     7321 cggcgacatc ttcgccgagc tcaaccgcct gcagcagaac ttcgagcagg ttttccggcg
     7381 cgagggcgcg cgcagcatac gcgccatgcc gcgcgacacc ttcccggtga tcaatgtggg
     7441 cagtacgccc gaagccatcg aagtgctggc gctggcgccg ggcatcgacc ccgccgcgct
     7501 gcagctcacc gtcgacaagg gtctgctggt cgtggccggc gaacgcaagg cagaacagcc
     7561 ggccaatgac aaggtggcgg tttacgccaa agagcgcttc gaaggcgctt tccggcgcgt
     7621 gatctcgctg cccgaagacg ccgatcccgc agccatcaac gccagctacc gcgatggcct
     7681 gttgcgcgtc acggtggcgc gccgcgagtc gtccaagccg cgccagatcc agatccactg
     7741 aacccctgaa cctttttatt tccttcaagg agcatgtgat gaaccagaac acagctttgc
     7801 gccagagcaa ccccgcccgc gatgtggcgc cagcgacccc ggacgcagac agcgcgcaag
     7861 gtgctgcatc gcagcgcacg acggcggtgc tgccgcctgt ggatgttttt gaagatgatg
     7921 ccggcttcac cgtgctggcc gacctgcccg gcgtggccag ggaccggctg gccgtgcggg
     7981 tggacggcga cagcctggtg attgaagggg ctgcggcggc gccggtgggg gccgacatga
     8041 cgctgatcta cggcgaggta ctgaacccgc tgtaccgtcg caccttcacc ttgagccggg
     8101 aactggaccc ggcaaagatc gaggcgaaac tcgacaacgg ggtgctcagg ttgaccattc
     8161 ccaaggccga agaggccagg ccgcgccgca tcgaggtggc cctggcctga atctcctgag
     8221 tctcctcctg aggctcttca gtgcgggcgc gtgcagtagg ccaggaactg gtcgagttcg
     8281 gtggacttgt cgaacaccgc atctgcaccg cccgccaggc agcgccggcg gatgtcatcc
     8341 gtggcgtagt tggacaacac caccacgcgt tgctgccgcg agcgctgccc gcagcccctg
     8401 agcacaccca gcccgctgcc ctgttgcagg aaaagatcga cgaccagcag ttgccagtcg
     8461 cgcgggtgct gggccagcca ggtgcaggca cctagttcgg tttccgcgta acccaccgtc
     8521 tgcacgccgg ccagctcggt caaggtttcc accaggttgt cccggatgac cgcgttgtcc
     8581 tcaacgatat aggctttcaa cgccgcgggg cgtcgggcgg gggaagggtg accggttccc
     8641 tggagggttc cggcggcaag gttcatggga gtcacgtagc cagtctcccg cgatgaccgc
     8701 gggactggcg caagggattg cactgcagcg gtgtaggaca gtgcgcttca agtgccggag
     8761 ggcctgtgcc gatcgccagg ggcgccggct ttgcatgccc ctacaggtac cagcccttgc
     8821 gctgcggacg cctcaagcta caaaaaaagt agcacctcgg agtggcggaa ctgtgccgaa
     8881 gctacagcgg acccgacggg ccggtcctgc cggccagccg ccggcgcaca tacacgccga
     8941 taaaaccaag gcccagcacc agtgcaaacc agcctaccag ggaagagttg gccacgatgc
     9001 cctgcatgcc cggcgaagtg atgaagtaag gcgataccag cacggccagg ccgatcaggg
     9061 cgcacaggag tcccttgacc aaaagttcgt tgttggcctt gttgctgtgc ctgcggatgg
     9121 gggggggagg taggggcatg gcctgattgt cggccaaatg cgaagggcag ggcgccccgg
     9181 ggggcttgcc ggcatcgcgc cggacggatc ggtgcaatgg acttatagtg gctgctacct
     9241 attttctttc ggtccgggac cattttcagg agctttttgt gtccaccacg cccgcagcag
     9301 ccattcaacc gacccgcttt ggcagcgggc aggccgttcg ccgcctcgaa gacgatgcct
     9361 tgctggccgg tgccggccag ttcaccgacg acgtgaccca gcccgagcag gcggccctgt
     9421 tttttgtgcg ttcaccgtac ccgcacgcac gcatcgtggc ggtggacagc gccgccgcgc
     9481 tggccatgcc gggggtgctg aagatcgtga ccggcgccga cctggcagcc gccggagtca
     9541 agccgattcc cggctccagc ggttttccca gggccgacgg ctcacccggg gtctcgcccg
     9601 tgcgccgcgt gctggcccat gaacgggtgc gtttcgtcgg cgaggcggtt gccgccgtgg
     9661 ttgcgcagac cctgcagcag gcccgtgacg cggccgaagc cgtgatggtg gactacgacg
     9721 agcttcccat ggtggtggac atgaacgacg ccaccgcgcc cggcgccccc gcactggtgc
     9781 cgcaagcgcc cgacaacatc gcctgcgaaa cgcgttatgg cagcgtggaa gccaccgccg
     9841 cggcctttgc caaagcccgc catgtggtgg cgctggacat cgtcaaccag cgcctacatg
     9901 cgttgagcct ggagccgcgc tcggtgctgg tcgcgtttga ccagacctcg cagcgcctga
     9961 cgatacgcat gagcacgcag atgcccaccg gtgtgcgcaa ctcggtgtgt gacgcgctgg
    10021 gcatggcgca ggagcaggtg cgggtggtgg tcggcgatgt gggcggcggc ttcggcatga
    10081 agaccggcgt ttaccccgaa gacattgccg ccgcgtattg cgcccgggcc ctgcagcgac
    10141 cggtcaaatg ggtggccgag cgcagcgagg aattcacgtc cgcgtaccac ggccgcgacg
    10201 tgcagagccg cgccgaactg gcgctcgacg agcacgggaa aatactcgcc ttgcgcctgg
    10261 cctcgctggc caatgtgggt gcgtatgcca ccggcgcggg tgtggccatc cagctgctga
    10321 tcggcccctg ggtgcagacc agcgtctacg acatccagac catagacttt catttcagcg
    10381 ccgtgctgac caacaccgca cccaccagcg cctaccgcgg cgcgggtcgg cccgaagcga
    10441 ttttcacgat ggagcggctg atggacgagg ccgcgcgcca gaccggcatc gaccgcattg
    10501 aactgcgccg gcgcaacttc atccagcccg cgcagatgcc ctacaaaaac cccatgggcc
    10561 aggtgtatga caccggcaag ttcgagtcgg tgatggacca ggcgctggtg ctggccgact
    10621 ggaaagggtt tgatgcgcgg gcggccgcgt cggccgcgaa tggaaaaatg cgcggcctgg
    10681 gcattgccac gtttctcgag tggaccggcg gcaatgcgct ggaagaaacc gtgacggtcg
    10741 cggtgcaggc cgacggcatg atcgaagtct tcaccgccgt caacgccatg gggcagggta
    10801 ttgccaccac gctgacccag ctggtggtcg atgcctttgg cgtgcccgcg gaccaggtgc
    10861 gggtggtgat gggcgacaca gaccgcggca acggttttgg cagcgccggt tcacgctcgc
    10921 tgttcaccgg cggttcggcg gtacgcgccg gtgccgacaa ggcgattgca ctgggcaagc
    10981 agcttgcggc caaggagttc gaggccgccg cggaggatgt ggtttacgcc ggtggcgact
    11041 tcacggtcgc cggcaccgac ctgcgtatcg gtctcttcga cctcgcaggg cgtcagagcg
    11101 accaacgcat ttttgtggat tccaccacca cggtggccgg gccgacctgg cccaatggct
    11161 gccacatcag cgaggtcgag gtcgatccgc agaccggcca tgtggcggtg gtggcctacg
    11221 cctcggtcaa cgacgtgggc cgcgtcgtca acccgatgat tgtgcgtggc cagctcgacg
    11281 gcggcgccgt gcagggcatc ggccaggcgc tgtgcgagcg catggtctac gaccgcgaga
    11341 ccggccagcc cgtgaccggc agcctgatgg actacgccgc gccgcgtgcc gacaccgcgc
    11401 ccgacttcag gaccgagatg gaccagtcca ccccctgcct gaacaacccg ctgggcgtca
    11461 aaggcgtcgg tgagctcggc accattggtg ccggccccac ggtggtcaac gccgtggccg
    11521 acgcactggc ccgccgcggc atggccgccc aggccagggc gctgcagatg ccgatctcac
    11581 cggcgcggct ctgggagctg atgcaggctc catcgtgagt gtgtgcgatt ctcctgattg
    11641 aaaataagct atagaccttg tgcgacgggc gtgagaagct atcaataaca gagcagtttc
    11701 aggcgccacc tgccacatgg tttccaccat gtgaatcttc tgcgtctgca tgaagtgctc
    11761 gttcacggcg gagcaaagca ggggtggtcg tggctggtcc tggccgtgtt gtttgacctt
    11821 gatcaaagtt tggtcggacc cctgcctgcg cggcaacaag gatgaagcca taatccaagt
    11881 aattgattaa ttacttggat gcccatgcat gcagaagcaa ccggcaagcg cctcaagcag
    11941 gccacggaag tccgccaggc tggcttggtg gaggccgccc tgcaactggc cgcgcagcgc
    12001 agcccggctg acatcacgac gggcgatctg gcgcaggccg tgggcatcag ccagggcgcg
    12061 gtgttccggc actttgccag caaggaggcc atctggctgg ccgtgctgga cggggcttcc
    12121 gacacgctga tggcccggct gcaaacggcc gctggcgcgg cgcccacccc gatggcagcg
    12181 ttgcaggcga tgttcatggc gcatgtggac tttgtggtgg cccatcccgg cgttccccgg
    12241 ctcatctttc aggagctgca gcacgcgcag gacaccgcac tcaaggccgg cgtgcgcagc
    12301 ttgatgcaga aataccggag cttgctcatg ggtgtgttgc agcaggcgca ggaccagcgc
    12361 ttgctggcgc cgggcacgga cctgcaggct gccgccgtcc tgttcattgg ttcggtgcag
    12421 ggcctggtca tgcaatcgtt gctcagtggc gatgtccgcg ccgtgaccac gcatgctcgc
    12481 ggcgtgctca cgatttacct gcgcgggctc gccgccggga gaggccggac agcggcgaca
    12541 gacagacatg cccccgcctt gaaggagacc ccatgaaaag caaacttccg ttttcacgcc
    12601 gcatcacgct gggcgccctg gtcgtggtgc tggtgggcgc gttggcctgg gtggcgctgc
    12661 gcaccggccc gctggcggcc acgcgcatca cggtcatacg ggtgcaggag ggccgtctgg
    12721 cccccgcgat ttttggcatc ggcaccgtgg aggcgcggcg cagctggatg gtgggcccga
    12781 cggcggcggg ccgggtgctg gctgtccggg tggacgtggg cgacacggtc aaggccgggc
    12841 agttgctagc cgacatggac ccggtggatc tcgaccagcg cctcgcggcc ctggatgcct
    12901 cgctggcccg cgcgggcagc acgcaggcag cggcgcaggc ccagttgaag gacgctgcgg
    12961 cccggcggga actggccgcc atcaatgcca gacgcaacga ggagctggcc gcgcaaaatt
    13021 tcatcagtgc cgctgcgctt gaatcccgca tgcaggaaaa agcgtccgct gacgctgctc
    13081 tgcaggcggc ccaggccaat ctggccggcg ctggccagga cgtggcccgc cagcaggcag
    13141 agcgc
//
LOCUS       DAIDFG010000052        13123 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_52_length_13123_cov_18.407025, whole genome shotgun sequence.
ACCESSION   DAIDFG010000052 DAIDFG010000000
VERSION     DAIDFG010000052.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 13123)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13123)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13123
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52_length_13123_cov_18.407025"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2365 a         3988 c         4197 g         2573 t
ORIGIN      
        1 gccatcacca gaaagaagat ggccagtccg atgcggccgc ggtacggacg caggaagggc
       61 acgaggcccg agagggagcg cggcgatccc ttgggcgcgt cggagggaga ggaagtggcc
      121 atgaggggag tttagtcggc acggccgggc gggctcgggc gagaaagccg gatggccttt
      181 tggcggcttg tcatagatca agcacgctgc cggggggcag cccacaatca acactgcagt
      241 gatcaccgcg cgttgcgctc gggttgtcag gctgcagggg gcggcaccgt gccgcgcaac
      301 acataaagga taaacacatg ctgaaaaaca aggtagcctt ggtcaccggc gcgtcgtcag
      361 gtattggccg cgccattgcg ctggtctggg cgcgcgaggg cgcccgggtg gtggtgtctg
      421 acatcaacga acaggccggt gaggaaacgg cctcgctggt acgggccctg ggcggcgacg
      481 ccatgtttgt ggcggccgac gtaggcaaac cgcaggacgg cgaggcgctg gtggcgcgca
      541 ccctggcgct gcacggccgg ctcgacatcg cctgcaacaa cgccggcata ggcggcccgc
      601 aagcccccac ggctgattac ccgctggacg gctgggcgca ggtcatcaac atcaacctgt
      661 cgggtgtgtt ttatggcatg aagtaccaga ttgccgccat gctcaaaagc ggcggtggct
      721 ccatcatcaa cgtcgcctcc atcctcggca acgtcggttt tgccggcgcc ccggcctata
      781 ccgccgccaa acacggcgtg gtcgggctga cgaaagcggc cgcgctcgaa tacagcgcac
      841 acggcgtgcg tgtcaacgcc gtcggaccgg gcttcatcca cacccccatg atcagcggcc
      901 tcgaagaaga ccccgccatc aacgccatgc tggtcggtgc ccatccaata ggtcgtctcg
      961 gccgcgccga agaagtggcc gagctggtgg tctggctgtc ctcggacaag gcttcttttg
     1021 tgaccgggtc ctattacccg gtggacggtg gttacctggc gcgttgagcg gccagccagt
     1081 gatccggacc ggaaaccttc cggtggatta cctgtagatc gacgggcccg cagccacgga
     1141 ttccgtgcct gcattcatga gtttcccgtc atctccggag ggaagtgatt cagccttcac
     1201 agggcgggtg gcttgccacc cgtcattctt ggtgtgcttg atgaaaaatg acaccatgcg
     1261 ctcccggctg tcttctgcgg cgactttgtt gaggcccacg agattgccca gatagcgctg
     1321 aggcacgata tcgagatcaa acgagtgagc cgcctcgggg taaatcttga tgtccagctc
     1381 cgccccggct gaacgaacac cctggcattc ctgggtcggt gtccagctgt cctgcccgcc
     1441 accaaatacc agcaagggtg acgccagatt caccctgcga ttgccgaatg agccacacca
     1501 cgggtaatag gccaccacgg cgcgataacc accttccccg gcggtttttc catgtggcgc
     1561 atcacccttg gcgacattga tggcgacact cccgccattg ctctgtccca tcagaaagat
     1621 gctgctggta ttcacaaacg tccgggtttt caggtagttc aatgcgtcgt aggcatcgtg
     1681 ggtacggtag tcaagcgcat cgacctgctg ggggacactt tcgcaaacct tgccgccacc
     1741 gttgttccgg ggaccaaagc tgtcgagggc aagaacgaca aatcccttgc caaccagata
     1801 gtccgcatag ccattcatgg tgtagcgcac cgccggttgc cagccgccgc agccatgcat
     1861 gagaaccacg gccgggaagg ggccatttcc ggggggcttc gatatgtcgg ccctcagggt
     1921 cacatgcgcc gacgtgtagg cacgactggc ctggaaatgc actttgtcgc cggcatgagc
     1981 ggcggctaca aacatcgacg gcaaacacca caaagcaatg taagccgacc gcctgcggtc
     2041 ggtccgcctc tcgacgaatg gcctccccgg agccgacgca gggctcccgg atttccttcg
     2101 ttgacttttc cgtctctcga aaacaggcgc cacctggacg cccgccatgg accatccggt
     2161 tttgtacaaa ggctgattca tcgtcccgat tccctcgagg tcatgtgatg gctgaatgct
     2221 tggcggggcg ctgtccttgc catcccgcca atatcttgat tctctccctt tcactgccag
     2281 ccttcaagct gtggctgatt tgaccgaatg caagccgtcg tcgaggctgc ttgcctgcac
     2341 ccggtggccc ggatgacgca ggccggccct ggccctgcag tcagcttcca ccggcggacc
     2401 tgcatgacct acatcaacac cctccacacg tcagacgttt aaccttcttc ctacagtttg
     2461 ttacttctag tagcagttcg cggtatctgc agctgtccct cggcatttac ggataacaca
     2521 aacgaaataa ggggcgagac acaacatgcc tgagaaaaag aaggaagcag ccaggacggg
     2581 cgcatcgcga cgggctggca ctttgcgttt ttacgatggt tcgttcgttc tgaacacaag
     2641 ctccggcatg ttttatcgac tgagcccggc agcagactac ctgcttcgtg cgcttgaagc
     2701 cggggcgaaa acaggtcaat ttgcggatct gatcgcaacc cgttatgggg tcgatcacgt
     2761 aactgcggtt cgcgataccg aactcttgtt gaaccagttc acagcgctgg ggcttctcga
     2821 caacttccag acgaagcgag ccccatgaaa agacagatga aagccgtcgc ggtcaccggc
     2881 ctgcaccgcg gcgaaaaccc ccagcctggt ccggcagtca tcgccagctt gcgccggcgc
     2941 ttcccggacc tgcgcattat tggtttgtcc tatgacccca tggagagcag cctgtatggg
     3001 caaggggccg atcatccgga tgcggcctac ctgattccat ttccgggtgc cggagcgact
     3061 gcgcttctgg agcgcctgga catgattcgc gagaaggagg atcttggatt tgtcatcccc
     3121 tgtctcgatt cagagctgga aaattacatg gccattcatc cccagctgct ggaacgggga
     3181 attggctgca ttcttcccac gccgaattcg ttcaaggcgc ggggaaaagc ggatctgtac
     3241 gatttttgcc ggcgcatcgg tgtgcccgtg cccgtgacga aactggcgaa caacccggct
     3301 gccgttgaaa gatgcgctca ggagatcggc tatccggtct acgtcaaggg caggctctat
     3361 aacgcgaatc tcgccaggtc gcgggcagac ctggcgcagg cctacgacga aattgtcaag
     3421 gtgtggggct ggccgctgat ggttcaggaa ctgctctccg gcgaggagta cgacgttgcc
     3481 ggcgtcggtg atggcaaggg acgcattgta aaaagctgca ccatccggaa gctgctccgg
     3541 acatcgcacg gcaagggatt cgccgggatt gtggtggagg accccgagat tgatcatctt
     3601 tcgcaacgca tcatccatga gctgcgctgg gatggcccgt tcgagctgga gtttctcaaa
     3661 gcccccggca agccccacgc actgatggaa atcaatccgc gtttcccggc gtggattgat
     3721 ttcccgtctc aaataggttg caaccttcca gcattgcttc ttgaccggct ccttgaccag
     3781 gaaccgcagc cgctgagaac ctgcgccgcc ggccagatgt ttgttcgcca ctgcgtcgat
     3841 ctcgtgggcg agttttccag ttttgccgac atggccagta gcggcgagcg cattttcaaa
     3901 ccccgttcag cccccataga gagcacttca tgaaccgcga ctatttaccg ccaagcattg
     3961 gccccgaact cggacgccag gcccttgatg cagctgccga ccgcaacctg tacgcacgcg
     4021 gtacgcgcgc accgaccttg ttcgagatag acggcattcc tgtggcgtcg ctcgttgaac
     4081 aattcggatc accactgttt gttttctccg agcatggttt gcgcgagaaa gctcgacgca
     4141 tgcgcgaggc cttcagaagc cgttatgagg acacaaactt tgcatggtcg tacaagacca
     4201 attacctcaa cgcggtttgc caggtatttc atcaggaagg ctggacggct gaagttgttt
     4261 cagacttcga atatgagaag gcccgcaaac tgggcatccc gggcaaggac attattttca
     4321 atggtcccta caagtcgcgg agcattcttg agcttgcaat cagcgaaggt gcactgatcc
     4381 agatcgacaa ctgggatgag ctcggggtca tcgaagaact cacccggcat ctcaggaagc
     4441 cgctcgacat cggcatacgc gtctggcttg atgcgggcat caggccggtc tggtcgaagt
     4501 tcggttttgc catcgaaaac ggcgaggccg gacgtgctgc agagaggatc ctgaaaaacc
     4561 cccatctgag gctgcatacc tttcatacgc atatcggaac ctacattctg gatcccaacg
     4621 cataccgtgt tgcggccacg aaacttgtcg ccttgcgcga gcagattttt gtcgagcacc
     4681 agcacctggt cccctgtctc aatctgggcg gtggctttcc atcccaaagt ctgctgcatg
     4741 gcatggaagg tccggccgag cgggtgattc cccccattga agcgtacgcc gatgcgatca
     4801 ccgaggtcct gaatcgtttg ccggcgaaaa aacggcccca actgcgcttt gagagcgggc
     4861 gtcatcttgt cgacaacgcc ggctatctgt tgagctcggt ggtcgccatc aagggggttc
     4921 gggacccgtt ggccgtcagc tcggacctga ccggacgcga ttacaaggag cagacgctcc
     4981 tgggcgaaga cgcactggca agttatgttc ttgatgcggg gatcaacctt ctctacacgg
     5041 cagcctggta ccagatcgac gcactcccgt cccgatcaat caacgcgcca ctttccacgg
     5101 cgcggcttta tggcccgctt tgcatggcga tcgacgtcgt tcgctaccgc atcgatcttc
     5161 cccctctcaa cgttggcgag gtggtcacgc tgtatccggt cggtgcctat aacctggccc
     5221 agtccatgca gttcatttcc tacagacccg ccgtggtcat gattggggcg aacgggaagc
     5281 cggagatcat tcgtacgcgt gaggtgctcg acgatgtcca gcgaccggag cggctgcctg
     5341 cccatctgga caagaagcct tgatgctcac agaagcccgc tccacacatg agcctgcacc
     5401 ggcggcggtc ggggtacacc ctcgccgcac gccaggcccc gtgcgcagaa aactggacgc
     5461 gtggctttct cctgcagggc ttgcgctggg gggggtgttg ttcattcccc atcccctgct
     5521 tggtgttttt ttgtggctgg ccctcgcgct caaccctccc tatgtcgtgt tcgccctttt
     5581 ggggctggtc attggtgctt cgatcaagcg cctgctccgc atcagtgatg gaccgatcct
     5641 gggcggcggg ctgaaggcca atgccttgct ggccgctgtc gtgacggggt ggatgaccag
     5701 cgggctgggg ctggcctgga ccccccggct ggcgatggcg ggcgcagcag ccatcgcggc
     5761 aaccctcgtg tcggccacat tgatgcgcgc tttctccaga agcattttcc cgccgctgct
     5821 ctgggggtat tgcctcgtgg cggtcatgct gttcagtgtc tgcccgcagt gcacggtgat
     5881 ttcggcgaac gccatgcaac cctggcctgc gcccgttgac gccctgggct gggtggagag
     5941 ctttctgcgg tcgatggggt cgctgatgta cctgcctgac gccagggtcg gtctgctggt
     6001 ttgcgtgggc gtgttgtgct ggtcccggac gatgttcctc aacggcatcg ttggctggat
     6061 aagtggcgta tgcgtggcgc tcgctttcca ggagatgtat ctggtttatt actggctgcc
     6121 cgtgtcgtac aactatttca tcgcgggcat ggcgctgggc gcagtgatct tcctgccggg
     6181 caggttgacc ctgctggtgg cggccgttgg cgggtgcgct gcttcattct ttgcgctggt
     6241 gctgcagtac acgacaaact ggtcggcgat tgcctacctg ccgatttctt cgggtgtggc
     6301 gatctgggtc ggtataggtg cattcacccg ggatcggtcc atcggactga gaaatctcat
     6361 gtcggagatc acgcccgagg agaagtggtg gcgggtggcg tattggtaca agcggttcgg
     6421 gccgtattac cccctctttg ccgtaccagt ggagggtgac ctgaaaattt cgcagggctt
     6481 cgatggcaag ctcagccaca tgggtgtgtt ttgccatgcg ctggatttcc agcgccccgt
     6541 ggcctccgga tccatctggg gagcccgtgt catctcgccg gtttcaggca tcgtcgagcg
     6601 catcaaaaac tcggtcccgg acaacgcgct gggcgtgtgc aattacgccg agagatgggg
     6661 caaccacgtt gtcatccgcc ttgatcgcgg tggctgtgct ctgctggctc atttgcagca
     6721 aggaacggtg tcagtcgtac cgggcacgcg cgtggaaacc ggaagttata tcgggctggt
     6781 cggcaactcc gggcgctcgc cggttccgca tctgcacctg caacaccagg acgcgcctga
     6841 gcccggttcg ccaacaacgc cattccggct ggccaatttc ctgtccggta accagaccga
     6901 aagcgcttcg tggtcatgga acgccgcggc tgtcccgggg gagggcgatg tcgtgatggc
     6961 ggcaccatcg aacagggtgg tctatcaggc actggcgggc atggttccgg gatccgcggt
     7021 ttgggttgtg gaagcccgcg ggcatattcc acggggattt cgacagcatt cttcggccgg
     7081 gaccttgcgg gttgacactc atctggacga actgggacag catgttttca gggcggggga
     7141 aaacggcagt ctggtctcca ggctcgatcc cgacgcctgg cgcctcgttg agctcaggca
     7201 ggtgacgtcg ccctttctca agttgctcgc gcttgctgtg ccatccatcc cgtatgcggc
     7261 caggcctggc atggcctgga gcgattttcc gcccatgatg ccgcctggtg ggctgttcgg
     7321 cccgctggtg ctggcgctgg ctccctactt caaacgccag tttctgaaag tgtcctgcgg
     7381 atgcatgccg acgcctgcgg aagccggcca ttcattccag atcgacagcc atgtgcagac
     7441 gcgctcgccg tccttgccgc tcacggtgtc ctgtgacttt gccatgctgc gtggccccgt
     7501 cagggtgcag gccgatttcc agggcgggac gctggtttac accttgctgt cgtacgagcc
     7561 gcgcgacctg gcgaaggagt gatgccatgg cccaagcctg acctgccccg cagtctttgt
     7621 tctgactgcg cttgctggcc acctgttttt tttgtttgcc gtctatcttt taaggagtat
     7681 gaaaatgctg aaatttgaaa aaaatcgtct gttgcctctg gccgcactgg ctttgcccct
     7741 gctgctggct ggatgcgcgg gtaacgtcct gcgggttgcg ccccacgctg aactcaaggt
     7801 cgtcgatccc gtctggacga cggcgtacat cacgcgcaat cacgggtacc tggtttatga
     7861 caccttgttt gcgatggatg aaaacttcga gccgaaaccg cagatggtcg acacgtggac
     7921 gatgtctgcg gacaagaagg tctggacatt caggctgcgt gacggtctga aatggcatga
     7981 cggaaaagac gtcactgctg ccgattgtgt tgcatcgctt cagcgttgga gcaagcgcga
     8041 cggcgtgggc cagcagatgt tcagtgatgt cgagagcctg acagccctgg atgccaggac
     8101 gatcagcctg aagttgcgtg ttcccaatgg gaacgtgctt gagacgctgg ccaaaatgtc
     8161 gtccaacgtg ccgttcatga tgcccgagcg ggttgcccag gccgatcctt cccagcccat
     8221 tcaggatacc accggctcgg gtcccttcat cttcaaaaag gaagactggg tgccgggcaa
     8281 aaaggcggtc tacatcaaga accgcaatta tgtggcgcgc gtcgagccgc tgtcgatggc
     8341 cgctggcggc aaggtccccc aggcagacat gattgagtgg cgctatttcc cggaccaggc
     8401 ctcaatggcc caggcgttga tcgacggaac agtcgactat gttgaatctc cgtcgaacaa
     8461 acttgttccc acgctcgaag gcaaaaagga tgtggttgtt gcgtccaccg atccgctcgg
     8521 gaatatcgga atggcccggt tcaatacgct gttgccaccg tttgacaatg tggaggtgcg
     8581 gcgtgctgcg gcgatggcga tgcagcagga agactacatg agcgcagcac ttggggatcc
     8641 gaaatactgg cgcacctgtt actcggtgtt tccctgcggc acggcctatt ccaacaacgc
     8701 tgccagcgac gtcatgaaat caaccagcct tgacgccgcc cggaaagcgc tgcaggcggc
     8761 gaagtatgac ggtacgccgg tcgttatcct caatccggtg gacagccccg tcatatcggc
     8821 ttttaccaag gtgaccgccg agaaattgcg cctgattggc atgaaggttg acgttcagga
     8881 catggattgg gcaacactga ctcagcgacg tgccaatcgc ggctcggcca aggacggtgg
     8941 ctggagcatg tttcacacct ggtggctggc cggtgatgtc atggaccctg gttttatcgc
     9001 cttctccggg gaccgtgaaa acggctggtt cggctggccg caggacgcgg aactcgagaa
     9061 atatcgcctg gccttcaaac aggccgccac tctggatgaa aagaaggtgc ttgctgccaa
     9121 agtacagcag cggctcatcg agatcggcgc ctttggcatc ctcgggcagt tcttcgagcc
     9181 ggttgccttc agcagccatg tcaccggtat cacatcgcct atccagttct actggaacat
     9241 gtcggtcgag ctgccttggt ggaagcgcat gctgcgccag gtcggcaaaa gctggatgta
     9301 agcgtcatgg gggtccattg gcaccgaggt gtcgatgggc ctcctggcag gccttggcgg
     9361 acccggccgg tgccgcgatg gctcacctga atatgtggac ttgagcagct atttatttga
     9421 tagctgcttg cgcttgttgg tcaagggctg aaggcctgaa aggcttgcaa acccggcgtg
     9481 atgccgggtt tgccctgcac aaggcaggcg gggccggcta tgcgcgcgac agccgccgcg
     9541 ccggctgcgc cccgccccgc ggcaggttgg cgctgaccag cgctgcggcc agcgtgacga
     9601 gccacgagta atacacccac accaggaaag ccagcaaagg cgcgaaggcg ccgtagatcg
     9661 tgcggtacgt gggcacgttg gcgaggtaaa tcgcgaagcc gtgtttgccc agctcaaacg
     9721 cgatgccggc gagcaggccg cccgcgatcg catcgcgcca ccgaacaatg gtgtgcggca
     9781 cgaagcggaa cagacaggcg aagccggcga cgctcaggac cacgggcccg aggttcaggg
     9841 catgcggcag ccaggcgggg cgcgttccca ccagtccgct ggaggcagcc agaagatagg
     9901 acgcggccca caggctcgca ccgatgatca ccgggccggt gagcagcatg gccacgtaca
     9961 gggccagccg tcgcagcacg gtgcgcggct tcttcacccg ccagatccgg ttgagcgtgt
    10021 tctccacgct cagtagcagc gtaagggccg acaccagcag gactgcgaag cccacctgcg
    10081 tgaggccgct ggcgttgccc gcgaattgcg ccaggtgttt cagcaccgtc ctggcgatgt
    10141 cggccggcag caggccgcgc agcaggtgct cgcgaatcgc ttccccggcc gcgcgcaggg
    10201 ccgggatgcg cgcgaacagg gcaaaactca ccgccagcag cggcaccatg gacaccgcca
    10261 tcgtgaaggt caggccgccc gccgcctggg cgaggcgttc ctcccggccg cgccggagga
    10321 tgcgggtgat caggttgccc tggtttcggc ttgcagtggt catggcagca atatagccac
    10381 gtccttgcag tttctggccc ggcgttgggg aaatgtggaa gcttgcttgc tcaaacgcgg
    10441 catccgcgcg acaaagcccg cccggcgatg acactgcccg gcgcggtggg cagcccgatc
    10501 ctgatgcgat cctgatgcgg caggtggtac gctggctgga gcatgaacac cccgggaaca
    10561 tcgcaaaacg gcagcccttc agcccgcaac tggctcgttg tggtgttgct gctggcggca
    10621 gccacagcgg tcgggctggt gctggacagt tacgtgacgc tgaccagcct ggccatggtg
    10681 tatgtgctgg cggtggtgat tgcggcctac cggctgggct ggatcgaatc gacggtgtgc
    10741 gcggtggggg cggtcacggc gctgaatttc ttttttgttc cgccgcgctg gaccttcgag
    10801 gttgaccacc gggagaacct gattgcgctg gccaccatgc tggtgcttgc cctggtcatc
    10861 agccggctgg ccacgggtct gcgccgcgag acagggctcg cccgcctcaa cgagcggcgc
    10921 gcgcgccagt tgcaggccct ggccacggcg cttgccagtg ccaactcccg ggaagaagtg
    10981 ctgacggtgg gccggacgac cctgaacgcc gcctttgccg gcccctgttt tctggtgctc
    11041 acaactgccg gcaacgagct ggaacacgcc gacgggctgg atgcttccgt gcgcgatggc
    11101 atgcggcaat gcatggccga tgcggcgctg ctggggccgg gcaccgggcg ctggccgggg
    11161 ctgaatgcct ggtacctgcc tctgggcgaa aaaggccgtg tgacaggggc cgcctgtgtg
    11221 cagccggccc tggccaggga cgaagacggc cgccagcatg cgcaggcgct gtgttcgctg
    11281 ctggcgcagg cggtgtggcg cctgcgcctg accgcgtcca tgctggccgc gcaaggcgag
    11341 gcgcagcagc agcaattgca gagcacattt ctggcggcga tatcgcacga cttgcgcacg
    11401 ccgctggccg cgattgtggg tgccgcctcc tcattgcaga cgcagcgcga caagctcagc
    11461 ccggccgagc aggaccgcct gctgggcagc attgccggcg aggccgccta cctgtccgcg
    11521 gtgaccgaga acaccctgca actcgtgcgc ctgagcagtt cggcgcagga actccggcgc
    11581 gactgggagt ccatggagga aattgtcggc gcggtgctgg cgcgggtgcg ccagcgcgac
    11641 ccgggccgcc gcatccggtc ccgggtgccc gaagggctgc cgctggtcaa ggccgatccg
    11701 gtgctgcttg cgcagctgct cggcaacctg ctcgacaacg ccctcaagta cagcgagggt
    11761 gcgattgacc tgacggtggg cgtcgagggc ggggaactcg tggtctcggt caaggaccgc
    11821 ggtgatggca ttcccgatgc cgcgcaggcc agcatttttg aaccctatgt ccggggcgac
    11881 caggtgggcc cgcgtggcgc cgggctgggc ctcgcggtgt gccgcgcgat tgcccaggcg
    11941 catggggggc gcctgacgct caggcgccgc cagggcggtg gcagctgctt ctccctggcc
    12001 ctgccgatcg aggtgcagcc gtcgcgggga gaagggccat gagcgcagcg cagaaccgct
    12061 tcaagccagc gaacaccccc tcggggggca gcgaggacac gccgtgccga gcgcgggggc
    12121 tgccatgacg cttcgtgtgc tggtggtcga ggaccaccgt gaaatacgcg cgctgatgca
    12181 atcgtcgttg tcggtggagg gcttcgaggt gcagaccgcc gtgtcactga gcgaagcagg
    12241 ggccatgctg cgccaccacc cgcccgacgt gatcgtgctg gacctgggcc ttcccgacgg
    12301 cgacggggtc gagctggtgc gtgaggtgcg caggcacaag gccattccca tcatcgtggt
    12361 gtcggcgcgc caccaggagg cgcagaagat cctgttgctc gatgccggcg ccgatgacta
    12421 cctgaccaag cccttcagcg tcggcgaact gctggcgcgc atccgggtgg cgctgcggca
    12481 tcgcggcacg gcgctggccg ccgcggtgac cgaacatgtg ctggacgacc tgcacatcga
    12541 cctgcagtcg catgtggtgc tgcgcgacgg ccagcccgtg cacctgacgc cgaccgaata
    12601 caagttgctg gcgcggctgg tgcgcagcgc cggccgggtg gtcacccacc ggcagttgct
    12661 ggccgatgtc tgggggcccg agttcaccga gcacacgcac tatctgcggc tctacatggg
    12721 tcagcttcgg gccaagatag agcgtgactc ggccgagccg cgccatctgc tgactgaaac
    12781 cggggtgggt taccgcctgg cgaccgcttg acctgctgcg cacttatgcg ttcctgatgc
    12841 cccggcggcg acagtgaagc cttgttcact gttgatggat ggttatgacc aagacttccg
    12901 gccgcgaaag catggcggcg ctcaccctgg gcgccatggg tgtggtgtac ggcgacatcg
    12961 gcaccagccc gctgtacacc atgaaggaaa ttttcagccc cgccaccggc ataacgctgg
    13021 acgcggcaca tctcatcggc gccacgtcgg tgatcttctg gggcctgatg ctggtcgtca
    13081 cgctcaaata cgtgctgctg atcctgcgcg ccgacaaccg cgg
//
LOCUS       DAIDFG010000053        13061 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_53_length_13061_cov_17.174074, whole genome shotgun sequence.
ACCESSION   DAIDFG010000053 DAIDFG010000000
VERSION     DAIDFG010000053.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 13061)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13061)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13061
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53_length_13061_cov_17.174074"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2130 a         4387 c         4272 g         2272 t
ORIGIN      
        1 gttggacagc acaaaacggt cgcggtcgaa ccagtgcggg ttatgcgggc tgtgcttgag
       61 atggcggccc cagagtgcga ccgccatgtc ggccatgccc atgggcgcgc cggggtggcc
      121 ggagttggct tgctgcacgg cgtccatggc cagagcacgg atggagttgg ccatgaggga
      181 ggtgggagtg tcagccattg cttgaatcca gggtgtgtga aggtcgcagc gcccggtgcc
      241 ggctcggcca ccggcaaaaa accgggcact cgggaaaacc cctgatttta cgggatgcca
      301 ccccgcgcgg tttgccggcg caccgccagc ctggagtgct atttatttga tagctgttgg
      361 cgcccggtat gtatgggcaa ggggccgttt tttcttataa gccggcataa ttcaggccac
      421 atgagcaccc aagccctgat tctggccgcc ggccgcggcg aacgcatgcg tccgctgacc
      481 gaccaccacc ccaaacccct gctgagcgtg cagggcaagc cgctgatcgt gtggcacctc
      541 gagcgcctgt cgcgtgcggg tgtcaccgac gtggtgatca acaccgcctg gctggaggaa
      601 cagtttcccg cggcgctggg cgacggcagc gccttcggcc tgcgcatttg ttactcgcac
      661 gaaggtgccc gttttggcgg cgcgctggaa accgcgggcg gcatcgccac cgcgctgccg
      721 ctgctgcagg acgcgccctt ctggctggtg gccggtgacg tgttcatgcc gggctttgat
      781 tttccggcgg ccgacgcgca gcgttttgcc gcctccagcg cactggcgca catctatctg
      841 gtgccgaatc cgccgcacaa ccccaagggc gactttggca tcagcgccgg cgggctggcg
      901 ctgtcgcagg ctgatgaaaa attcacctac agcaccgcgg gcctgtaccg cccggccctg
      961 ttcagggaca cgccggccgg gcagaaagcc gcactggcgc cgctgctgcg ccaggccatg
     1021 ctaagcgacg atgtcaccgc ggcgctatat tcaggcgcat ggaccgacgt gggaaccccc
     1081 gaacgcctgg ccgagctgaa cattccccgt taggaaaacg cttttatcca tgaccaccat
     1141 ctacgaaaaa cgccgcgccg ccgttgcacg cgcccttaag gccgctggcg gtggcatcgc
     1201 cctgttgccc accgcccccg aagtggcgcg caaccgcgac agcggctttc cctaccggca
     1261 cgacagctac ttttattacc tgaccggttt tgacgagccg ggcgcctggc tggccatcga
     1321 gtccagcggc cacagcacgc tgttttgcca gcccaaggac atggagcggg agatctggga
     1381 tggctttcgc ctcggcccca aagccgcacc ggccgcgctg gccgtgcagg cggcgttcag
     1441 cgtagacctg ctggacgaaa aaatgcccgg cctgctggcc aaccagcccg ccgtgtggtt
     1501 tccgtttgcc acgcacaagg gcctggaaac ccaggtcgac ggctggctcg ccaaggtgcg
     1561 cgcgcgcatc cgcttcggcg ccgaatgccc gcaaagccag catgaccttt gcaagctgct
     1621 tgacgagatg cgcctgagca aggatgcgca cgaggtggcc atcctgcggc gcgccggcgc
     1681 catctcggcc ggcgcccatg tgcgcgccat gcagacttcg gcggccatgc tgcgggctgg
     1741 cgtgaagggc gggctgcgcg agtaccacct cgaagccgaa ctgctgcacg agttccgccg
     1801 ccacggctcg cagtttccgg cctacaccag catcgttgct gccggcgcca atgcctgcgt
     1861 gctgcattac cgcgcgggca atgccgagct caagcctggc gagctgtgcc tgatcgacgc
     1921 gggctgcgag ctcgacggct atgccagcga catcacccgc accttcccgg ccaacggcaa
     1981 gttcacgccg gcccaacgcg tgttgtatga aatcgttctg gaagcgcaaa acgccgcggt
     2041 gaaggtgacc aaacccggca agcgcttcat ggacccgcac gacgcggcca cgcgcgtgct
     2101 ggcccagggc atgctcgaca ccggcctgct gtccaaagcc aaacacggca aagtcgacga
     2161 tgtgctggcg tcgggcgcct accgccagtt ctacatgcac cgcaccggtc actggatggg
     2221 catggacgtg catgactgcg gcgactacac cgaaccgtct agccgcccgc gcgaggaaaa
     2281 agacgcgctc ggccagaccg tgatgcgcaa gccctcacgc atcctgcgcc ccggcatggt
     2341 gaccaccgtc gagcccggca tttacgtgcg gcccgccaag ggcgtgccca aggagttctg
     2401 gaacatcggc atccgcatcg aggacgatgc gctggtcacg gccaagggct gcgaactgat
     2461 gacgcgtggt gtgccggtgg aggccgatga gatcgaggcg ttgatgcggg ggtaaggtga
     2521 ggtcaggtca cgcctccgcc cttcacgcaa gccaacacgg gatcaaccga tcccggcccc
     2581 acccaacacc ttctgcgccc actgcggcaa gggattgacg ttctcctgcg tccagccacg
     2641 cgtgcagaca gccaccgccg tatgtgttcc cggcttcacg tcctgagagt cgtacaaaat
     2701 ggccaagtcc gccagacgca cagccaccgc gaggtgggcc agggtgcgcg gatagcgggc
     2761 aagtattcgt tcggccggta cggcatgacc gccttcggtg acacgaccgg caacacgttc
     2821 cagcaggcgc tccggatcgt ccagacacac gacgatcagc atgacgaaaa acccctgttt
     2881 ttgtgcctct tcgatcagaa cgagcttgga ttcatgggag aacaccgttt cgctgacaaa
     2941 cgactggccg gccttgagca attgagcacg tcgcgcatcc gcccattgcc ttgcgtcctc
     3001 tgacctggcc tgcggattgg ccatgtgctg caagtgagcc gcctcataca ggtcagcgtt
     3061 gacgaattcg gcacttacag gaagggtgcc ggcccgcatc agtgccttgt agagcgtaga
     3121 cttgcccgcg ccgttgggac cggccagcag gtagaagacc ggtttggctt tgcgccgccg
     3181 cgatcgcagt ggcgtcacct gccccatgtc aggccggcgc cttgcctgcc ttgctgcggt
     3241 tatgggcaat gacgtcgcgc acatgctgtg caaggctgcc atccgcttct acagccatga
     3301 agcgtcccat cagctcatcg acctgcccgt cgttttttct ggccttgggc cgtgcctgca
     3361 acgcagcggc ctcataaact tcaatcgccg tctgcgcctc ctgcaccgtc aggccggaat
     3421 gctcgaccac ccgacccaaa gtggcccagt attcgatctg gcccgcggcc gaacggcgca
     3481 ggggctgggc cgcttcccgc gcttgttcca ccaggccgat gggcagtttg acggaggcaa
     3541 agttgctgga cgtggccatg agaatctcct tattggcgca ttttgcgcca aaatggaaag
     3601 agctgcaagg gcaaaagatc tcttgatcgg gccagcgctg gcgcagcggg gctgcagatg
     3661 gctgatttat tgctagtctg ggccatggcc ccttccacct tccccgccaa agccaccgcc
     3721 gacccgcagt ggcgcgccca ggagctgctg ctcatgagcg aggtcatgcg cctggtcggc
     3781 aaaagcctgg cgccggaggt ggtgatgcgc gagatgctgc acctgatgtc cgaactgctg
     3841 ggcctgaacc gtggccgcat cgtgctggcc aactttgtgg gtgacctggc gctgcaaggg
     3901 ctggcggacc gcaagccggc agaccgcgcg gccgaggcgc cgtcgcccgc ttcggccatc
     3961 cgttatgcct atggcctgac acggcaggaa atggcacgtg gccgttacgg gccgggcgaa
     4021 ggcatcaccg gccgggtgct ggccacggcg cagcccatca tcgtgcagga catcgacgcc
     4081 gagccgcagt tcctagcgcg gtcggtcgcc agggcacagc tgccaccgga caccgtcgcc
     4141 ttcatcgcgc taccgatcga ggtgaaccgc gaggtcatcg gcgtgctggc ctgccaccgc
     4201 atccgcagcc gcgaccggca gctggcggac gacgtggcgg tgctgaagat cctggccacg
     4261 ctggccggac agctgttgca gctgcaggcg ctggtggccg acaaaacgcg cgccctggag
     4321 gcaaaaaacc gtctcttgag ccgcgcgctg gaaaccgcgg cagcgcgtta cggcatcatc
     4381 ggcacctcgc cggcgctgct gcaggcgctg ggcgaactgg agcgtgtttc cgaggctacg
     4441 gccagcgtgc tgctcctcgg cgagtcgggc accggcaagg agctgtttgc acgcgccgtg
     4501 catctgtcca gccagcgccg cgaccagcct ttcatcaagg tcaactgcgc ggcgattccg
     4561 gacacgctgt tcgagtccga gctgttcggt tatgagcgcg gcgctttcac cggcgcgcaa
     4621 aatgcgcgcg ccggctggtt cgagcaggcc gaccgaggca cgatttttct cgacgagatc
     4681 ggcgagatgc cgctggccat gcaaaccaag ctgttgcgca cgctgcagga gggaaccatc
     4741 gtgcggctgg gcggcaaacg cgagatcaag gtggcagtgc gcgtggttgc cgccaccaac
     4801 cgcgacctcg cgcaggacgt gctggccggc agcttccggc gcgacctgtt ttaccggctc
     4861 aacgtgatcc cgatccgcct gccctcgcta cgcgagcgtc ggcaggacat ccgcgcactg
     4921 gccgtgcatt tcttgagccg catcaaccag gccaaccagc gcaatgtgag cctgtctgcg
     4981 ggggcgctgg cgcgtctcga acaacacccg tggcccggca acatccgcga actgggcaat
     5041 gtgatcgagc gtctggtgct gctgagcgac agcgccatgg tctccgccga agagctggag
     5101 cggtttttgc cgcacgagca agaccaccga acgccgggcc agccggcggc caccccgcaa
     5161 accgctgttt cggtgcctgt ggtccgggac tacctggccg cgcaatcgca cccggcggcg
     5221 cagctgcagc aggccttgcg ggaccatggc ggcaaccagt cacgcgccgc ccaggcgctg
     5281 gggctgacgg tgcggcagtt cagctaccgc ttgcgcaaac tgggtctgca caatgtcgac
     5341 aatgtgtaga cattgtgtcg tggctggcgg gcaagcgggt tttccggata cctgatttgc
     5401 tactaaatag atagctgttt gcgcttgtaa ataaagggct gcaggctttt tcagctcgaa
     5461 ttcccagccg gagaaagaca tggcacggcc gttgcaataa cagtggggac ggcatgtgcc
     5521 gtgacctcac ctggagattg ccatgacgac cgacagcgtt cttgtttccc ccgaacaact
     5581 ctcgcacatg atgggcaccg agcccgtggt catcatcgac acacgcgacg ccgacaccta
     5641 tgccgccggc catttgcccg gcgccgtcaa catgcgcgag atcttcactt tcctggccac
     5701 gtccacggcc gaaggcctga ccgcgctcaa gtccaccttt gccgaacatt tcgggctggt
     5761 gggactgtcg ggcaaggaaa ccgccgtgtt ttacgaagat gcgctcaaca gcggctacgg
     5821 ccagtcctgc cgcggctact acctgctgac ctggctgggt taccccaagg tcaaggtgct
     5881 caacggtggt ttctcggcct ggaaagccca gggcctgccg gtcagcaccg aagcggcaac
     5941 gcccacgccc gccaccttcc cggccaacct ggccatggcc gacgtgatgc tgaccaagga
     6001 ggatgtggcc gccgcgcttt acaccgatgt cgcgctgctg gacgtgcgcg acatcgacga
     6061 atggaccggc gaaagctcgt cgccctacgg caaggacttc gcgccgcgca agggccgcct
     6121 gcccggcgcc aggtggatag agtggtaccg cttcatgaaa ccctcggccc agggcccggt
     6181 gttcaagtca ccgctggaag tgcaggccga atgtgccacc gccggcatag gcacggacga
     6241 cacggtgtac ctgtattgct tcaagggcgc ccgcgcctcc aacacctttc tggcgctcaa
     6301 gcaggccggc ttcagcgacg tgcgcatgta cttcggttcg tggaacgagt ggtcgcgcga
     6361 cgactcgctg ccgattgaaa gcgacgcacc ggtgatgaaa gccaaacagc cgtcgctggc
     6421 cttggccgcc tgaacggggc cctgccgccc cccttctttc gctttcttct ttcgctttcc
     6481 cttcttttgt tgacaacctg atcaggagac ttcccccatg tccgtggaca ccgccatcgc
     6541 ccccgccgca ccgaccaccg tgaaggccta cctcaaaccc atcgacggcg agggcttggc
     6601 cgcctttgcc gccaagggtc gtgccaaccc ggcctcgcgc ggcaccaaca aggtccacac
     6661 cgtgatgcag ggcatgtacc gctcgctcag ctacgtgggt gaccacacgc cggtggtggt
     6721 ggatgaaccc ctgcacctgt tcggagaaaa cactgcgccg gcacccggcg aaattgtgct
     6781 gtcgggcctg ggcggctgcc tggccgtcgg catcaccgcc gtggcgacct ggaagaaggt
     6841 caagctgtcc aggctggagg ttttcatgga aggcgacatc ggcaacccgg cggcgtgggg
     6901 tgccggcggt gccgccaagg aagcgcccga catgggcttc caggcgatcc gcgtgaagat
     6961 cgtgatcgag ggcgatgcac cgcgggaggt gctcaacgaa attgtccagc atgcgaactt
     7021 ctattcgccg gtggccaaca gcatgcgcaa cccgattccg ttcgagatcg ccctggccga
     7081 ctgaccccgg ctctgccccg cacgaccgcc ccaccaccgc aaggacttcc tcatgaatcc
     7141 cgccgacctt ccccgtgaac tcacctgcgt gccgccacca gagaccgatg ccctgctggc
     7201 cgccgtgcgc gcggtcgcgc aggggccact gaagcaacag gtggaagcga ttgaccggca
     7261 aggccaatac ccgcgggccg tgctgcagga gctggcgaag ctgggcgcga tgagtgcaca
     7321 cctggatacg cccgcaggcg ctggcagcta cgcgctggcg atccaggcca tggccgaggt
     7381 ctcgcgcgtc tgcggcgcca ccggcttcat gatgtggtgc caggccgtgt gcggcctgta
     7441 catgcagcag tcgggcaacc cggccctgat gggtgaggtg ttgctgcggc atgccagtgg
     7501 cgccggcctg ggtggcacgg cgctgtccaa cccgatgaaa agttacgcgc agatcgaaag
     7561 cctgctgctc aaggccacac ctgtcgatgg tggctacctc gtcaacggca cgctgccctg
     7621 ggtcagcaac ctggggccgg accactactt cggcgcgatt gcagcggtga tggcgggcga
     7681 tgcacctgcc gctgcaacag gaagcggcgc ctgcgcgtcg cgggaggtga tgttcctgct
     7741 gcgctgcgac gcccccggcg tggagttgcg cgagtgcccg agtttttcgg ccatggaggg
     7801 caccggcacc tacggcgtgc gcctgaagga ccatttcatt ggcacgaacg aaatcatggc
     7861 cgatccggcc aagccttaca tcgcgcgcat ccgcgccgct tttgtgatgc tgcagtgtgg
     7921 catggcagtc ggcatcaccc agggcgcgat cgactcgatg tgggccgtgg agtcccagct
     7981 gggccacgtt aaccagttcc tggaggaccg gcccgacacc ctgcaggccg aactggacgc
     8041 gctgaccggg cgcgtgatcc aactggccga aacgccgttt gaaccgtctg ccgaattttt
     8101 cattgacgtg ctcgatgccc gcgcccacgg cgccgagctg tgcctgcgcg ccgcccagtc
     8161 ggcgctgatg caccaggggg cacgcggcta cctgatgtcg tctgaggtac agcgacgcgt
     8221 gcgcgagtcg cattttgtcg ccatcgtcac acctgcgatc aagcacctgc gcaaggaaat
     8281 cgcgcggttg agcgccgagg agcagccgga atgatcctgc cgccgcaagc acggcctgtc
     8341 gcggccgtca ccaccgatgc gtccggcttg ccctgggcgc tgtatatctg caaggcctgc
     8401 ggactgattt atgacgaagc cctgggtgat gccgacagcg ggctggcgcc gggcacacgt
     8461 tttgccgaca tccccgacga ctgggcctgc ccgctgtgcg gcgtgggcaa ggccgacttc
     8521 gaaccccatg tggtggccga gcttccgcag cgacgggcaa ccagcacggc ctcccgtctg
     8581 ccccaaggcg ccgccgcgcg gcacaacgcg ggcacggtca tcgtcggggc agggcgcgcc
     8641 ggctggcaga tggcgcaagc cctgcgcgaa cgcgacgccg acatgcccat taccctggtg
     8701 accgcctgca acggcgacat ctacgacaaa cccatgctgt cggtagccat cgcacgcggc
     8761 atggccgtcg acaagctggc gcgtgaaagt ggcgaccagg cggcggagcg cctcggtgtg
     8821 cggctgctgg cccacacgca ggcggtcagc atcacgccgg gtagccgcca gctgcgcacc
     8881 acccgcggca cactgcgtta ccggcacctg gtgctggcgc atggcgccca ggcgcgctcc
     8941 ctgccgcaac tgcctgcggc cctgggctgg cgcatcaacc atcttcaggc ctacgccaag
     9001 ttccgtgcgg tgctgggcac ctccccgcaa cgcatcgcca tcgtgggcgc cggcctgatt
     9061 ggctccgaac tcgccaacga cctggcgccg gcgggccacc aggtcaccct gctcgatgtg
     9121 cagacgcggc cgctcgccag ctgcctgtcg gaagcgcaat cgcagcaact gctggcggcc
     9181 tggcgcgacc tgccgctgcg gtttgtgggc ggagtgcaga tcagcggggt gacgtcgctg
     9241 gcggcggcaa gccaaggggg gaaacagctc accacgtcat gcggccagac cttcacggtc
     9301 gaccacatca tcgtggccgc agggctgcag acaccgagcc ggctggcgcg cagcgccggc
     9361 ctggcctgga accagggcat tgatgtccag ccggactcgc tggccaccag cgtggaaggc
     9421 attcacgccc tgggtgattg catttccgtc aacggccagg tcagccgttt tatcgagccc
     9481 atcggccggc aggcgcaaac gctggcggcc ggtattctgg gccaggccgt caccccctac
     9541 catcctgtgc gcgtgccgct gcgggtgaaa acgacgtccc agccgttcac gctgtagccg
     9601 gccgcgtcaa cctggctgat gtatcggctc cacgggcaag cgctcgacaa tgacccggcg
     9661 cgccggcgac ccgtcgccct gcgcctggga aatggtcagc accgccgaac gagcgcctgc
     9721 actcacccga atctgcagcg gcagcgcgcc ggacggcacg cctgcggcga caaaggcctg
     9781 ggcctgagcg tcgtaccgca accacgccgg cagcggccgg ccgctgaggg tcgtggcctc
     9841 gaccggcaca gcaccttttg tcgcccctgc cagcagggcg ggcggcagcg agaaacgaaa
     9901 gcccttgccg gcggtgacca gctcgctgac caccgtgaca gtgacgaggc cggcctcctg
     9961 gtcggtgggc tcgcgcacca ccttcaccgt cgttccactg ccgccgcctg aagcggtcgg
    10021 actgctttca agcagggcat tcgcgtccgc caccgtcagg ccggaagcgg cgaaccgctg
    10081 cgggggaacg ggcggcagcg tgggtgtctg gaaaaaggag ccggcgaagc tggacaggtc
    10141 ggtgatgctg gcggtggtgg tggccgtggg atccatcagc gtgtagttgt ctgccgccgc
    10201 accgcccagg gagatgccgg tgatgtaaac ggttttgttg ccgcccaccg acgggttgtc
    10261 aaaattgccc gtcgctgtgg ccacatggag cacatccccg gccaccagat tggggaaacc
    10321 ggcgccgccg gtgttgaggg tggcgctggt attgccgtcc accaccttgt tgtaggccgt
    10381 gatgccggtc acgcggggca tgcttcttcg cgtgatgctc agcaggccat tgttcagcac
    10441 cagcgtgtag ttttcactgt tggtggcgcc tggcgtgatg gttaccgggc cggcggccac
    10501 cggcgtgttg aacaccgccg atgtgctgcg gtaaacccca acgctggcgt ccgtgtcgcc
    10561 atcaatcaag ccggtcgtgg gattgccggt gaacacggga ttggcgtcac catacacgcg
    10621 ggtggcgttg tcggcactga tggtcagggt aggcgcgatc cggtacagga acatgttggc
    10681 gctgccgccc ggggtgaaac tggaagacgt gttgtagcgt ttgttgtacc ccgccatgcc
    10741 ttccgtcgtg gtagccgggt cgccgctgta cgccagataa cgtccggcgg aagccacgat
    10801 gccgctggcg cccacgctgt tgaccagctt gccgcccgtg ctggttccag ccgcattgct
    10861 gctgcccgcc ttgagcacaa tggcgtcgcc cgtgccgttt gaagtcaccg cattggccag
    10921 cgtgagatcc gacgcggcgc cggtggtttc aattttggcg gcaacggctg tggtgacgcc
    10981 cgccacaccg gccacagtcc ccacggtcag cgcgccggcc tggctgaaat cgatgctgcc
    11041 ggtattggcc gccagcgtgg tcaccgcatt gccggcatgc cgcagggtgt ggttgccgcc
    11101 tgtgcccgtc agcgccagac ctgatgcgct gatcgcgccc gattgggtca ccgtggcacc
    11161 ggccgagagc gtggcattgc cggtggtcag cactgccgcc acatcgaagc cattgacgtc
    11221 ggtgtattga atcgccccgg ccgaggtcgc ctgaagattg atcgttccgg cgttgttgcc
    11281 gctgttggtc agtgtgatgg gcgagttggc tgccgagttc tgcactttca gcgtgcctgc
    11341 cgtgatgttg ccggtggagg cggtctgtgt gacggcgccg ttggccagga ggtcgagcgt
    11401 gcccgtgaca ttcgatgctg cggtaccgac acccatgttg atcgcaccag tagcgacgaa
    11461 ggcactgcca cccgccgttg tgtttacgcc cagattggtc gccgccacgt taatgcggtc
    11521 gccgctgctg ccaatgttgc cgttggcatt gagggtgaca tgggtggcgg taatgagttg
    11581 cgtgccggtg tcggtgattg accccgcatt gagcgtcagg gtcttgccgc tcagcccaat
    11641 gttgttgcca accgtcaaga caccgctgct ggctgtatcg ccgatgacga tggagcctgt
    11701 ggcgccggtc gcaatcgccg ttatttccgc gcctgaaata tcaaaggcgg aagcgcctgc
    11761 cagcgacatc gcgcgatttg cagttttggc cttgatggtc aacgcgccac tggtggcaag
    11821 tgcgttgttg ccggtgttcg tgccaatcgc aaccgtgtcc ccgatgactg tgatgggtcc
    11881 gctacctgcg gcaacgtcta tgtttttgtt ctgggtataa gtgcccgtgc cggcgtccac
    11941 aagaaccgtc ccaccagtgc ccactgcagc gccgcccgtt gcgctaccgc ccacggcgcc
    12001 gaggttgctg gccagcgtga tgcccagccc ggcccccgcc gcatcgagcg tgatggcccc
    12061 ggcattgccg cccgccgcca ccccggccac gccgttgccg ccggttgtgg tgattgcccc
    12121 cgccgccaca ctgccgcccg tggagctgac actcaccgca gcgccgttgc cgccggcctg
    12181 gttggcgcct gcgccgttgc cgcctatggc gctgatggca ccggtgccgc tgatggcccc
    12241 tgtcgaactc agggtgatgg ccccgccgtt gccgcccgcc cccagtgtgc tggcgttcgt
    12301 gccgttggcg gtgatcgtgc tggcgccccg cgtcagggct gcgccgctga tgttgatggt
    12361 gccgccgttg tgcccgctgg agctgccgag cgctgcaccg ccgctcgctg caatgtttcc
    12421 agccaccacc gtgccgctga ccgctgccag tgtgatgttg ccgccgtttg cgctggcacc
    12481 tcccgtggtg gtcagcgtcc ctgtcgtcag gttgcctgcc gagttgctca cgctgatgga
    12541 gccggccgtc ccgcctgtac ccgatcccac tgcagcaccc gtctgcgcgg ccagcgcgcc
    12601 caggctgatt gcgcccgagc cgctgttgga aaccgtgatg gctgcggcgt tgccgccgtt
    12661 ggcgttgccg gtgccgccag caccgccggt ggccgtgacc gtggccgcgg gggcagtgat
    12721 gccgcccaca ctggtgaggc tgaccgctgc ggcattgccg cctggctgac tggcgccgct
    12781 gcccgcgctg ccactggcgc tcacattgcc cagcgcggtg atggccccgc ctgtactgat
    12841 cgtgatcacg ccggcgttgc gccccgccgt attggcaatg gccgcaccgc ctgtgctggc
    12901 gatcgtgctg gcgctcaagg tcccggtggt ggtcacactg accgcgccgc cgttgccgtt
    12961 ggtcccgccc gccgtgctca aggcgcccag ggtggcgttg ctgcccgtca gcgtgatcga
    13021 gccggctgcg ccgcccgtgc ccgtacccgt ggccgctccc g
//
LOCUS       DAIDFG010000054        12999 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_54_length_12999_cov_17.896863, whole genome shotgun sequence.
ACCESSION   DAIDFG010000054 DAIDFG010000000
VERSION     DAIDFG010000054.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 12999)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12999)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12999
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54_length_12999_cov_17.896863"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2158 a         4038 c         4326 g         2477 t
ORIGIN      
        1 cgatctgggt cttgaagccc ttgcgctcgg cgtacttgag gtactggcgc agcagcatgc
       61 tggcccagtc gcaggcttcg gtgccgccgg cgccggcctg gatgtccagg aaggcgttga
      121 gcgggtcggc cgggttgttg aacatccggc ggaattccat tttctcgact tccaccgcca
      181 ccttggcagc gtcttcctca atggccagca tgccggcgtc gtcgctttcg gccttggaca
      241 tctcgaagag ttcggtgttg tcggccagct cggagctcag gcggtcgatc acatggacga
      301 cgtcctcgag cgactttttt tccttgccaa gttcctgcgc gcgcttgggg ttgtcccaga
      361 ctttcgggtc ttccagcgcg gcgttgactt cgcttaattt acgttctttg gcagggtagt
      421 caaagatacc cccgaagctc gttgacccgg ttgctcaggt ctgcgagctg gttgccgatg
      481 atattgatgt gttctgcgtc catgggaaag tcctgtcctg gtatctgggg ttcaatccag
      541 cattttctca tgctccgggc cgcgcggccg cgcggccgca aaagggccat gcccggcgca
      601 accccatggc tgcgtccgcc cgcccgggga tcaggccgcc ggcgagcccg ggggcgtcag
      661 gggcagggtg acggtgaaca cggagccctc gccctggcgg cttgccacgc tgatgctgcc
      721 gccgtgcgcc tgcacgagct ggcgggtgat gaacaggccc agaccgagcc cgccggtgcc
      781 gtcgtcgctg gcgacgcgcg agaactgctc gaaaatgcgc agctggtcgg gcgcggaaat
      841 gccccggccc tggtcgcgca ccgaaatgca cgcactctcg ggcagcaggc gcaggctgac
      901 ttccaccggc ttgccttcgc cataacgcag cgcgttggtc agcaggttga tcaccacctg
      961 ctcgatcctg aattcgtccc acagggcggt gaccggctgg ccggcatcga gggtgatggt
     1021 gctgccggcg gctgcggcct ggtgggtgag gttgcccacc acgcgggcaa gcagggccga
     1081 cagttccgtg ggcacgggcc ggatggacaa ctgcttgctg ttgatgcgcg aggcatccat
     1141 catgtcgtcg atcaggcgca tcatggcttc gatctggcgc cggtcacgcg ccaccatggc
     1201 gttcagatag ggcaggtcga aaatgtccgc acggccccgg tccagctgca tcttgcgcaa
     1261 ttgcgactcc aggtacagcg tgttgagcgg cgtgcgcagc tcgtgggtgg ccatggagat
     1321 gaagtcgtcg cgcgtgcgca cggcggcctg cagctcgtcc tgcatggcag tcaattgctg
     1381 catcagcaac tcctgctcct tgcggctctt ctccagcgcc tcgacctgcc ggcgggtttc
     1441 catgcgctgg ctgtacaggt ccacaaagac actgaccttg ctgcggacgg catccacgtc
     1501 cagcggcttg tagagaaagt ccactgcgcc ggtttcgtag ccggtgaagg cgtagttctg
     1561 ttccttgccg gcggcagtga cgaaaatgat ggggatctgc cgggtctttt cggtgccgcg
     1621 catcagctcg gccagctcaa agccgctcat gcccggcatc tgcacgtcca ggatggccag
     1681 cgcaaactcg tgttcgagca gcaggtccag cgcttcctcg ccggagctgg cctggaagat
     1741 ttcccggccg tcgcggcgaa tcagcgcatt gagcgccagc aggttgccgg gcaggtcgtc
     1801 gacgatcagg acttttgcaa cgtcaggtgt tcgcatggtt gtggtcaagt tggatgagca
     1861 gttgccggat gcggtccagt gtgaggatca ggttgggtct gagcttgcgg atggccgctt
     1921 gcggcatggt ggcgacctgg gcttccgccg ggtcctgcac cgctgtgaga ccgccgcgct
     1981 gcttgatgcg ggccaggccc gccgcgccat caaaattggc gccggtcagc agcacgcccg
     2041 ccaggctggt gccataggcg tcggcggcgg actccatcag cacatcgatc gatgggcggg
     2101 aatagttgac gggttcttca cagctgaggg aaaaagtgcg gtccgtctcg atggacaggt
     2161 ggtagccgga gccggcgaaa tacagggtgg acggggcgat ggtttccttg tcggcggctt
     2221 cgcgcacggt catggccagc ctttgctgga agacgtctgc cagccggctg tcacgctctt
     2281 gcggcagatg cagcaccgcg atgatgggca gggaaaagtg ctccggcagt cctgacagca
     2341 gcgccagcaa ggcgtgcacc ccgccagccg aggcgccaat caccacggcc tccacggcat
     2401 gccttgctgc gacctgctgg atatccatga agctgctcag tcctccagct tgcggaaaat
     2461 ccgctcgggc cggtccagcg gctcaaaacg cgcggcatgg gccgagaagt cgatgctttc
     2521 cttgctgccc aggcccatga agccgcggcg gctcagcgat tcgtgaaaca ggcccagcgc
     2581 gcggtcctgc agcttcttgt tgaaatagat cagcacgttg cggcagagta ccagctgggt
     2641 ctccgaaaac acgctgtccg tggccaggtt gtggtcggcg aaggtgacgt tgtcgcgcag
     2701 ggacttgtcg aaaatcgccg catcgtaagc ggccgtgtag tagtcggaaa atgcctgctg
     2761 gccgccggcg gcctggtagt tggcggtgtg gccctggatg ctggccagcg aaaaaatgcc
     2821 ctggcgtgcc ttggcgagcg acttggggtt gatgtcggtt gcataaatca gggagcggtc
     2881 gagcaggcct tcctccttga gcaggatggc cagcgaatac acctcttcgc cggtgctgca
     2941 gcctgcgacc cagattttca gcgagggata ggtgcgcagc acgggcacga cctgctggcg
     3001 cagggccagg aaaaacgagg ggtcgcgaaa catctcgctg acgggaatgg tcaggtactg
     3061 cagcagctgc atgaagacgg cggggtcctg ctccgtggcc gcctgcaggg ccgggaccgt
     3121 cggatagccg agctgcgcga gcgccttgag cacccgcctt ttctgcgacg cacccgcgta
     3181 atcgcggaaa tcgtagctgt actggttgta gatggcctgc atcaggcggt gcaggtcggc
     3241 ttccgggtgc tcctcggcgg caccgctgac gggggccggc gtttgggcgt caggcttttt
     3301 gggcattcag ctgcgatccg tggccggcat ccacacgcga agcagggaaa acaggcggtc
     3361 caagtcaatc ggcttggcca ggtagtcact ggtgccggct ttgaggcatt gctcctggtc
     3421 gtccttcatg gccttggcgg tgacggcaat gatgggcagc tttttgaagc gtgcatcctt
     3481 gcggatgcgg cgcgtcgcct ccagcccgtc catgcccggc atcatgatgt ccatcaggac
     3541 cagatcgatg cctgcgatct cgtcgagctt gctgatcgcc tcgaagccgt tgcggccgat
     3601 ctcgacccgc atgccttttt gctccagcgc gctggtcagg gcaaagatgt tgcgcgcgtc
     3661 gtcgtccacc accaggacct tgcggccttc aaaggcggtg tcccggctgc gcaggctctt
     3721 gagcatgatc tggcgatccg ccgacagttc cgactcgacc ttgtgcagga agagggtgac
     3781 ttcgtcgagc agccgctcgg gcgagcgtgc gcccttcatg atgatggcgc gcgaatactt
     3841 cagcagttcg gtttcttcct gacgggtcat ctctccgccg gtgtagacga tgacgggagg
     3901 gtaggcagaa atcccctcgg ttgacatgcg ctggagcagc tcgtggccct gcatgtcggg
     3961 aaacttcagg tccatgatca tgcagtcaaa aacggtgctg gtgagcagcc ccagcgcttc
     4021 accgcctgtc tccaccgcgg tgatctcgac gtcgccgtca ccgatcagtt gcacgacgct
     4081 ggcgcgctgt agcgcatcgc cttcgaccag cagcacgcgc ttgatcttct gggccagctt
     4141 gtcgcccagt ttctggaaaa cgtccttgag ttgctcgcgc gtggtcggct tgagcgcgta
     4201 accgatggcg cccatctgca gggcttcctc gctgaggtcg tcgcccgaca ccacgtgcac
     4261 gggaatgtgt cgtgtcttcg ggttttcctt gagccgctgc aacaccgaca ggcccgggcg
     4321 gtccggcaga cccatgtcga gcaggatggc atggggcagg aactgcatgg ccagggcaaa
     4381 accgtcttcg gcgccgtggg ccacgaggca gttgtagttc atttcacgcg ccaggtcgta
     4441 gaggatctgc gcaaaaatga cgtcatcctc gatcaccagg accaggcggt cgccgcgtgg
     4501 cagctccctg tcgtcggcaa aagccgcggc gggaacgggc gccggcaggg gggcgggcgc
     4561 catcaccggc gccgggttgc ggagccggcc gggcgcgcgc cgggcgggtt tggtgtccgg
     4621 ctggggcgtc ggtgccgggc tccattcgca gggcagcagc agggtgaagg tgctgccccc
     4681 gccctcgctg ctttccaccg agagcgagcc gcccagcagc gtggtcaggt cgcgcgaaat
     4741 cgacaggccc agcccggtgc cgccataacg ccggctggtg gtgccgtcgg cctggcggaa
     4801 ggcgtcaaaa atcagttgct gctggtcggg gcggatgccg atgcccgagt cgcgcacgga
     4861 aaaggcaatg ctgccgtgcg gctgcagcga cacgcgcagg ctgacctcgc cggcatcggt
     4921 gaacttgatg gcgttggaga gcaggttttt caggatctgc tcgacccgct ggcgatcggt
     4981 tttcagcgtc gcgggggtgc ctgattcgat gcggatggag agggtcaggt ttttctggcc
     5041 ggccagcggt tcgaacgtgc ttttcagcga atccaccagc gcgctcacga ccatgtcctc
     5101 gggggaaagc tccagcttgc cggcttcgac cttggaaata tcgaggatgt cgttgatcag
     5161 gctgagcagg tcgttgcctg ccgaataaat ggactgcgca aaccggacct cttcctggct
     5221 gaggttgccc ttggggttgt cggccagcag cttggcaaga atcagcgaac tgttcagcgg
     5281 cgtgcgcagc tcgtgcgaca tgttggcgag gaattccgac ttgtagcggc tggcccggct
     5341 caactcggtg gcgcgttcct ccagcagggt ctgggcctgg ttgagcgccg cattcttgtg
     5401 gtccagcgac agggccactt ccgacagctg ctcgttggtt tgctcgagtt cggcctgctg
     5461 gttctccagc gtgacctggt actccttgag cacgcgggac tgctcttcca gttcctcgtt
     5521 ggtggtgcgc agctcttcct gctgcacctg cagctcttca ttgagctcct gggactggct
     5581 cagcacgtcc tccaggcgcc ggcggtaaag cgccgcgtgg atggagttgc cgatgttgct
     5641 ggcaatcatg gtgacgaatt ccaggtcgcg tggcgccagg gcccgcagga aaccgagttc
     5701 gaccacaccg ttgaccagcc cgtcgttgtg caccggaacc accagtacat ggcgcgccga
     5761 cccctggccc aggccggagc tgaccttgag gtagccggcc ggcagatcgt caagctgaag
     5821 cacccggttt tcaagggccg cctggccgac caggccctgg tcgctgtcca gcgactgccc
     5881 ggcctcgtcg ctgtcatggg tcaggccgta ggcggccacc cgctgcagcc ggccgtcttc
     5941 ctggcggata tacagtgccg cgactgccac atcgagatac cgcgccagaa actccagcac
     6001 gctgcggctc agcgacccaa tggcgtgctg gcccagggtg tgctcggcca gctgccgttg
     6061 tccggtgcgc agccagagct gctggtccag cagcagcgcc gtggcttttt gcttgttttc
     6121 cgcttctccg taagtgtccg acaggtgctg cagttcgcgg cgcccgagca gcgccagcag
     6181 gccgctcagc cccaggctga acagcaggta ggcggccatg ccccacgccg tgttgctgcg
     6241 cgcctgcgcg ttgcggtcct gcagcaggcg ctcttcaatg ttgatgaatt ccgcgagttc
     6301 gcgccggatc tcgtccgtca ggcttttccc gcccgcagaa cgcaccggcg cctggtagtc
     6361 gaggtccttg cgccgcaggt cgacaatggt ttgcgcaaac tcgttccact gtgtctgcag
     6421 tgcggcgatg cgcctgagcc ggctgacctg gtcctggttg tcgcgcacca ggccggccag
     6481 cgtggccgtt tcggcggcga tgcgcggttt gccaatttca aagggttgca gcaggggttc
     6541 ttcaccggtg accaggtagc cggacatgct ggcttccatg tcggcgccca gcttggcgat
     6601 ctggttggca ttgctgatgg cccgttcggc ctgttcgacc ccgcccagcg cggacaggag
     6661 atagaagatc aggccgacaa aaaccgcggc actgagggcg ccgacgccca gcggcaggct
     6721 gacattgcgc gtcagaatgc ggcgaaagtt ttcctgctga atggcgctgg gtgcggtcat
     6781 gaatcttccc cgcgccaagc gcaataaagc cctgattgtc ctacaaagcg ggcttttctt
     6841 gtgtgcgtta ccgaacttaa accttacctg aggggctttt cgggctcccg tacatcagac
     6901 gctagctgag gaagtcttcg aacaggcggg ccagtggctc cggctggtcg tggtgcagca
     6961 tgtggccggc gtccacaatg acggcggtgc gcacctgcgg cacggactgc aggcgctcgt
     7021 ggtactgggc cagcgtgtat ttgccctccc accatttgcc cagggaatcg tccgaggctt
     7081 cgaccgccag cgtgggtgcg ctgatgcgct ggtaaatctc cagcacctcg tcgacccggt
     7141 aaagctgggc attggtgatc ttgtgggccg cgtcgcccag gatgcgccac tggccctggg
     7201 cgtcgggcgc agcccagtgg cgggccagcc agtcggcctt gtcctggctc agccgggggt
     7261 tggtgcgcat caggcgccgg gccacgccgt ccacgtcgtt gtagggcttg agcgccatct
     7321 cgcccttgtg caggcttttg agctcgtcca tccatttggc aaaacgggcg ggcgcctggc
     7381 tgggccgggt cgcgggcatg ccgaagcctt cgaggttgac cagtttgcgt atgcgttcgg
     7441 gccgcacgcc ggcgtagagc atggccacgt tgccgcccat gctgtggccg accaggtcca
     7501 ccggctggcc gggtgcatag tggtcgagca ggaaatcgag gtcggcgagg tagtcgggaa
     7561 accagaagtt gtcggccccc gcgctgccgg tctggccata accgcgccag tcgggcgcga
     7621 tgatggtccg gccctgcaca aaggcgtccg acagggcatc aatgacaaac tggtaggacg
     7681 cggccacgtc catccagcca tgcaccaaca ccagaggtgg caggctgctt tgggcctcgc
     7741 cccagtgaag cacgtggtaa ttgatgccgc gtatagggac atactcgctg cgggaaagac
     7801 gtttttcttt gtacattgat gcccatcata aggagacccg atttgaagcg cagtcaagac
     7861 agggacatgg ccgccgcgcg gcagcccgac cattacgcag ccctgcacca gggctttcgc
     7921 tggcaggtgg acgaatactt caacatcgcc gacgtctgct gccggcgctg ggtggccggc
     7981 agcggtaaaa gtgctcccaa aagagtagct atccgcgccc atcagaaggg ggctgcgacc
     8041 actttttata cctatgtcca gctgcagcag gccgccgacg cgctgagcca tgtgctggtt
     8101 cagctgggcg tgcagcgcgg cgaccgggtg gcgattgtga tgccgcagcg tttcgaaacc
     8161 gccgtggctt acatggcggt gttccagatg ggtgccgtgg ccatgccgct gtccatgctg
     8221 ttcggccccg aggcgctcga gtaccgcctg caggacagcg acgcggtgct ggccatctgc
     8281 gacgaaaact cgattgccag cgtcaatacc atccgcagcc actgcccggc cctgcgcacg
     8341 gtgatcgcgg ccggcacggc ggccggccag ggcgacgtgg actacgaacc cgctctggcg
     8401 gcgcaacaac gcgccttcac cgccgtgaaa accaaggccg acgacgcggc catcctgatc
     8461 tataccagcg gcacgaccgg cccgcccaag ggcgcgctgc tggcgcaccg tgcgctgatc
     8521 ggcaatctgc cgggctttgt ctgcagccag aactggtttg gctttgacgg caagaccaat
     8581 gcgacgtccg aagcggtgtt ctggagtccg gcggactggg cgtggaccgg cggactgatg
     8641 gatgcactct tgccgacgct ttatttcggc cggcccatcg tcgccttcaa cgggcgcttc
     8701 agccccgagc tggccttcac gctgatggcg cagcagggcg tcacgcacac ctttctgttt
     8761 ccgaccgcgc tcaaagccat gatgaaggcc tacccgcacc cgcggcgcca cttcaagctc
     8821 aagctgcagg ccatgatgag cgcgggcgaa gcagtcggcg acgcggtgtt tgcgtactgc
     8881 cgcgacgagc tcggtgtact cgtcaacgag atgttcgggc agaccgagat caactacgtg
     8941 gtgggcaact gctcggccct gtggccggcc cggcccggca gcatgggcaa gggctacccg
     9001 ggccaccgcg tggcggtgat cgatgacgag ggccgggagt gtgctgtcgg cgtgccaggc
     9061 gacgtgtcgg tcaaccgctt tgacatccac ggcgatgcgg acccgatctt ctttctgggt
     9121 tactggaaga aggaagccgc cacgcaggcc aagttcagcg gcgactggtg ccgcaccggc
     9181 gacctggcga cccgcgacga ggacggctac ctctggtacg aaggccgggc cgacgatgtt
     9241 ttcaaggccg ccggttaccg catcggcccc ggcgagatcg agaactgcct ggtcaagcac
     9301 ccggcggtgg ccaatgccgc ggtggtgccc aagcccgacc gcgaacgcgg cgccgtggtc
     9361 aaggccttca ttgtgcttgc tcctgatttt gtagctgctc gcgcacgaca ggcgggggct
     9421 gagggccagt ttgacatcaa gctcacggcc gagttgcagg cccacgtcaa gggcctgctg
     9481 gcgccctacg aataccccaa ggagatcgag ttcattgacg cgctaccgat gaccaccacc
     9541 ggcaaggtgc agcggcgcgt gctcagattg caggaagaag agcgctttcg ccaggcgggc
     9601 tgagcagtca ttctttacca gcaggggccg cagatctggc tttgccagtc tgcaggcgcc
     9661 gtcccctgga aggggagagg gcgctacgcg tagcgagcgc caacaggggt gaactgagcc
     9721 cttcggggca gggtgacggt aaacgtcgtg ccgctggtgg cgtccgaggt gacggcgatg
     9781 cttccgccgt gcgcacgaac aatttcctgg cagataaaca gacccaggcc cagaccggtc
     9841 ggctcccgtt gctcggcctt gccggaggcc atgccgcgga ccagcggctt gaagatcagt
     9901 tgctgttcct tcagcggaat cggcgtgccc aggttgtgca ccgaaaaggc cacgccttct
     9961 gcgttttccc acaggcgcac cgtcaccggt tcatctggcg cgccatgggt cagggcattg
    10021 cccagcaggt tggacagcaa ttgccccatg cggccctcgt cccagaagcc gttgaagtcg
    10081 ccggtgctct ccaggctcag caggcggcgc gggtagcgca cgcgtgtctc cgccagcacc
    10141 ttgccgaact gggcgagcag gtcgcccggc accggcctga tcggcatcat gccctcggat
    10201 tgcacccgcg tgaaatcaac cacgtgctcg atcacgctgt tgatgcggac cacgctgttg
    10261 atgatggttt ccgccgcact ggccgccagt tttccggagc gcaccaggta ttgcgccgac
    10321 atggaaatcg tgcccagcgg attgcggatg tcatggccga ggatgccgat aaacaggtcg
    10381 gtcgattttt tggtctggtc ggtgtagcgc gccacggatt cggcaatcgc ctggtccatc
    10441 gcttcattga accggatgag gtcgtgcatg tcgtttcggt gatcctgtcc gccgcccgcc
    10501 agggtccaga gccggacgac actggcccgc agggcgcggt attcggtgat catggcgtcg
    10561 atggcgaagc cggagaccat gcgtgtgtcg gcgtgggtct cggccgctgt ctcgagctct
    10621 ccctggggct gcctgctttc gccccgggat ttttcttcct gcgcggcagc ggtctggggc
    10681 tgctccagat cattggcaat cgccgcgagg atttcacctg cgtgatcgcg caaggctttc
    10741 tggtccagcc tggttccgtc atgcaccacc gtggcggcga actcgtccca cgaggtgagg
    10801 atttcttccg cgttggatcg gagaaatgtg gcaagtcgca tgagggagcc tctctgatgg
    10861 gggaacgata ttcaggctat cgcgggtgct tgcggcagct tgtctgcgtg ccggcgcacc
    10921 ctggaataaa aaaaaccctc cgggacaccg gagggttttt ttcagccggg acaggcgctt
    10981 agcgggcaac agcctgcacg ggctcggcca cataaatctc gacgcggcgg tttttggcgc
    11041 ggccttcgtt ggtgttgttg tcggcgatcg gctcgcgcga accgcggccg tcggtggcca
    11101 cgcggttgcc tgcaacacca cgggccacca ggtaattgcg cgtgctgttg gcgcggtcga
    11161 tggacagcgg gttgttgatg gcgtcgctac cggtgctgtc ggtgtgaccg atgatggtca
    11221 ccgtcgtcac cgggttctgg ttcaggctgg tggcaaactg gttcaggatg ggtgcgaagg
    11281 tggtactgac atcagagcgg ccggttgcaa acgagatgtc cgaggggatg tcgagcttga
    11341 ggcggttgtc ggcggtctgg ctcacagcca cgccggtgcc gaccgtggcc tgttccatgg
    11401 cagccttttg ttgctgcatg cgctgcgacc acagataacc gccgccagcg ccgacagcgc
    11461 cgcccagcac agcgccggta gccgcgcctt tggagccgcc ggtggctgcg cccagcacag
    11521 cgccggcaac agcgcctatg cccgcacctt tggccgtacc ctgctgggtt tcgctcatgt
    11581 tggcgcaacc gctcagaaag agggcggcac aggtcaggga agtgaggacg agtttcatgg
    11641 aaggctcctg ggaagttgtt gtagaagaaa gatttagagt ggattaacgc tttagtgtgt
    11701 gaatcgtatg aggtcttctg gtatgtattt gtaagcgaat cgcccgaaat cctgcccgcc
    11761 agcagcgatg ctggtgaccc cttaaactag ccgcggcctt ccattcaacc catttcatct
    11821 tccatttcaa tgcaaaaagt acagctaggc agcagcgacc tgcaagtcac cccggtttgt
    11881 ctgggcacca tgactttcgg cgagcaggtg ggcgaagcca cggcacattc gattcttgac
    11941 cgttccctgg agcgcggcgt caatttcctc gacgcggcgg aaatgtattc ggtgccggcc
    12001 cgcgccgaaa ccttcggcgc caccgaaacc atcatcggca actggctggc ccggcgcccg
    12061 gacgcccgtg accaactggt ggtggccacc aaggtcgccg gccccgcacg cggcatgccc
    12121 tggatacgca acggcagccc cgacctgacg ccgcaggaca tcgtccaggc ctgcaacgac
    12181 agcctgaagc gcctgcaaac cgatgtcatc gacctgtacc agatccactg gccggcgcgc
    12241 aatgtgccga tgtttggctt ggcgtatttc gagcccgcca aagacaaggt ggtttcttcg
    12301 attcatcagc aactcgaagc gctggccggc ctggtgaaag ccggcaaggt gcgtgccatc
    12361 ggcctgtcca acgagacccc gtatggcgtg catgaatttg tgcgcctggc ggagcaacac
    12421 ggcctgccca aggtggcgac cgtgcagaac ccgttctgcc tgatcaaccg cacggtggaa
    12481 aacgggctcg acgaaaccat gcaccggctc ggcgtgtcgc tgctggccta ttcgccgctg
    12541 ggttttggcc tgctgaccgg caagtacgac accagcggca ccaccggcgc cggcgcgccc
    12601 ggggacgccc gcattgcccg ctacgagtcg gtgcgcaagc agcgctgggg ccggcccgaa
    12661 gcgctggccg ccgcgcgccg ctacaacgcg ctggcccgcg aacacggcct gacgcccacg
    12721 cagctggcac tggctttttg ctacaccaag tggcaggtgg ccagcaccat catcggggtg
    12781 acctcggtgg cccagctcga cgaagacctc gacgcctggg gtacgcagct gcctgccgaa
    12841 ctgctggcag cgatcgacgc gatccgctgg gacatgcgag atccggccct ctgatcgagt
    12901 gcgggaaggc aatcaatgac cagctacccg ttcccaaatc agctggggcc gcggaaccgg
    12961 cttcgccggg ccgcaggcgc agcggccccc tcggggggc
//
LOCUS       DAIDFG010000055        12803 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_55_length_12803_cov_17.315893, whole genome shotgun sequence.
ACCESSION   DAIDFG010000055 DAIDFG010000000
VERSION     DAIDFG010000055.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 12803)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12803)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12803
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55_length_12803_cov_17.315893"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2330 a         4088 c         4094 g         2291 t
ORIGIN      
        1 ccggtgccca cttcctgctg gtgcgacaca aaggtgtagc cgcgatcgcg tgctgcgaac
       61 tcgggctcct gcaccttctc gacataggcc gacatgccgc gcttgacgta gtcctgggcc
      121 aaatcgaaca tgttgtacca catggagtgg atgccggcga gcgtgatgaa ctggtacttg
      181 taccccatgg cgcccagttc tttctggaac ttggcaatgg ttgcatcgtc gaggtttttc
      241 ttccagttga acgatggcga gcagttgtag gccagcatct tgccggggtg caccttgtgc
      301 acggcgtcgg cgaacttctt ggcaaatgcc agatccggcg ttccggtttc gcaccacacc
      361 aggtcggcgt aatgggcgta ggcaatcgcg cggctgatcg cctggtcgat gccctttttg
      421 gtcttgtaga aaccttcaga agtgcgctcg ccggtcagga aaggcttgtc gttctcgtca
      481 tagtcgctgg tcagcaggtc ggcggcttcc gcatcggtgc gggcaatcac cagcgtgggc
      541 acgccgcaga cgtcggcagc catgcgcgca gcgatcagct tctggttggc ttccaccgtc
      601 ggcaccagca ccttgccgcc catgtggccg catttcttga ccgacgccag ctggtcttcc
      661 cagtgcacgc cggcagcgcc ggacttgatc atggccttca tcagttcgaa ggcgttgagc
      721 acgccgccaa aaccggcttc agcatcggcc acgatgggcg cgaagttgtc gatgtagcct
      781 ttgtcgccgg catcaatgcc cttggagtgc tggatttcgt cggcacggcg gaaggtgttg
      841 ttgatgcgct caaccacctg cggcacggag tcgaccgggt acagcgactg gtcggggtac
      901 atggacgagt agctgttgtt gtcggcagcg acttgccagc ccgacaggta gatggctttc
      961 acgccggctt tcacctgctg catggcctgg ccccccgtca gcgcgcccag gcagttgacg
     1021 tagggctcgg tgttgaccag gtcccagagc ttttcggcac cgcggcgggc cagcgtgtac
     1081 tcgatcggga aggagccgcg caggcggatc acgtcggcag cgctgtaacc gcgcttgatg
     1141 cccttccagc gggggttggt cgcccagtct ttttcgaggg cggcaatctg ctgttcgcgg
     1201 ctcagttgtt cggtgagggt ctgtggcatg aggtcactcc ggaggttgaa aaatgggggg
     1261 acaaacagcg ctgcactatc catgcctttg ccgtttgctc tgggatcaac tttaagtctt
     1321 ctacaagact ttgtgaagct cttatgtctt atataagaga aaatttattt ccaacaaaat
     1381 caatggcctt tttacaatat ttcaatatat gaaaagtatt ttcttgtatt gagaaatatt
     1441 actgcgctgc agcattttga aatttcgtaa tacggaaggc ttttaccgga tcatgaaatg
     1501 cggggttgct accttccagc tggctcatcg ctggtggcaa acggcggatc aagccaagca
     1561 gaccatcccg caaacggctg cagtcctccg tctcacgtca cccgccaccc gctgcctcag
     1621 ggacgctgcc gaagatggtc gagccagtcg ccaccggtga tcaccgattg gcccaccgcg
     1681 cgggccgggt tgatttcata aacgagacag tccagttcca ccgcgccaga cagcgcctgc
     1741 ggctgatcca gccgcaccct caccgcctgc ttgcggtact cgccggtctg ctgcggccag
     1801 acctcctcga tgacgtcgag ctggcgctcc agttcggcgc tgatggtgta aacctcgccg
     1861 gtcacgcgag ccgggccgcc cagcaccacg cccgggtaag gcccgaggtc gtagagcaca
     1921 ccggccacgc tggccaggcc gacaaactgc ggcgccggtc gcagccgcgt gatgtcgcgc
     1981 acatcgccgc ggcgcagcgt gccgtacaca aagacatggc ggctggcgga aggcggcgtc
     2041 ggttcaggca tgaaagtaag gcatgatttc gggcaagcag aaaaagcgac ctggaagaac
     2101 aagcatcccg gactctgcgg cgcacagcag ccaacagcgc tgatggcagc cgcaggggcc
     2161 actcaaccct caacggaaaa cccggtggaa tgaaccgcat tgtggcctac gcctgcctgg
     2221 cgctcagcat gtccctggtc ggcagttatg tcgcgctgtc caaaccgctg gtcgcggtct
     2281 tcccggtctt tctgctggcc tggctgcgtt ttggcatcgg cggggcggcc atgctgcact
     2341 ggctcaaaaa gccgcccggg gaagtcccga tgacgcggca aacccggcaa ctggtgtttc
     2401 ttgaatcctt tctcggcaat ttcctgtttt cgatctgcat gctgttcggg gtgagcctga
     2461 gcagcgcggt ggctgccggc gtcatcatgg ccgccatccc ggccgcggtg gcggtgctga
     2521 gccggctctt tctcggcgag cgcatccgcc cccgggtcgc gctggccatt gcctgcgccg
     2581 tcttcggcat catgctggtt tcgctatcaa aaacagagct gtctgcgcaa gtacagaaag
     2641 ggctggaggc cgatttggca tccagccggg catggctggg caacctgctg ctggtcggcg
     2701 ccgtgctctg cgaagccgct tatgcggtga tcggcaaaaa actcaccggc gcgcttggcc
     2761 ccaaacgcat caccgcgctg atcaacctct ggggttttgc gctggtgaca ccgctgggcc
     2821 tgtggatggc ctgggggttt gactttgccg ccgtggcagc gggcagctgg ctgttgctgc
     2881 tgttttatgc gctggccgcc agcgtctgga ccgtgtggct gtggatgacg ggcctgaaag
     2941 gtgtgcccgc cagccaggcc ggggtgttca ccgtgatgct gccggtcagc gcggcgctgg
     3001 ttggcgttgg tgtgctcggc gaatccgtgg gcgcggcgca gctggccgcg tttgcactgg
     3061 cgctggccgg cgtggcactg gccacgctgc cggagaaaat gcgcccctga acgcagcagc
     3121 cccagggagc gtcagacttc aggcaaacag ggccttgtgc tgctcgcgca ggacgttttt
     3181 ctggaccttg cccatggtgt tgcgcggcag ctcgccaacg atgaagcagc gcttgggaat
     3241 cttgaagttg gcaagctgcg ccttgagccg cgcgatgatc tgctcggcgt ccagcgtcgc
     3301 gccggccttg gcgatcacga tggccacgcc gacctcgccg aagtccgggt gcggcacgcc
     3361 gatcaccgcg ctttcagcca cgcccggcat gtcgttgatg tagccctcga tctcggccgg
     3421 gtaaacgttg tagccgccgc tgatgatcag gtccttgctg cgcccgacga tggtgatgta
     3481 gccacgctcg tcgatcttgc cgacatcgcc ggtcttgaag taaccatcgg cggtgaactc
     3541 ttccttggtc ttctcgggca tgcgccagta gcccttgaag acgttcggcc ccttgacctg
     3601 gatgccgcct atctcgcccg tgagcagcgg ctggccggca tcgtcctgca cccgcaggct
     3661 cacgccgggc aaggcaaacc cgacggtgcc gccgcgccgc tcgccgtcgt aggggttgga
     3721 agtcagcatg gcagtctcgc tcatgccgta acgttcgaga atcgtgtggc cggtacgctc
     3781 ctgccattcg gtgaaggttt caatcaagag cggcgccgag cccgcgacga acaggcgcat
     3841 gtggaggcag gcctcgcggg tcagcccggg ctcggccagc aggcgcacat acagcgtggg
     3901 cacgcccatg aacaccgtgg cctcgggcag cttctgcacc accagcttgg ggtcgaactt
     3961 cgccagccag atcatcttgc tgccgttgat cagcgcaccg tggatcgcca cgaacagacc
     4021 gtgcacgtgg aagatgggca gcgcatggat cagcacgtcg ccgggttgcc aggcccagta
     4081 gtccttgagc accagcgcat tgctcagcat gttgccgtgc gagagcatgg cgcccttgct
     4141 gcggcccgtg gtgccgctgg tgtagaggat ggccgccaag tcgtcggcgc cagactgtgc
     4201 gatttcgtgc cggtcgctgc aatgtgcggc gcgggccaag agcgagccgg tgcggtcgtc
     4261 gtccagcgtg aatacgttct gcgtgcccgc cttgaaggcg atcttgctga cccagccgaa
     4321 gttctttgag ctgcacacca ccaccgaggg ctcggcgttg ccgatgaaat actcgatctc
     4381 gccgctctgg taggcggtgt tgagcggcaa aaacacgtaa ccggcccgca gcgtggccag
     4441 gtacagcatc atcgcttcga ccgatttttc aacctgcacg gcaacgcgcg cacccgccgg
     4501 caggtccagc gactgcagca ggttggccat catggcggtc gatcgttcga ggtcgcgcca
     4561 ggagtagtgc aggccggtgt cggtttccac cgccaccgca tcgaggtctt tcggaaaggc
     4621 tgcgcgcagg gcggcaaaca ggttgttgtt tttcatcgcg tggtacctgg gaatcgaaaa
     4681 agtcagaggt cgcccgccca actaaaaatt tggcggacgt ttttcaacaa aggccgacac
     4741 accttcgcgg tgttcggcgg agtcggcgta ggcatacgcc tttgctatca gttcagcagc
     4801 aggctgcgcc cgcccttcct gggccagagc ccgaaaagtc tgtttattca ggcgtgccgc
     4861 cgcgggcgcc agcctggcca cacggccggc cgtcgccagg gcctgcgccg ccacctcgcc
     4921 gtccggcaac acgcggttca gaaagccgcg ctgcagcaag gtggacgcgt cgagcaccgc
     4981 cgctgccagc agcatctcgc gcgccgtcaa ctcgcccgcg gcgcgcagca cgagcgcggc
     5041 ctcacgcggc gccatcggaa aaccgagctt gccgatcggt gcaccaaagg tggcggacac
     5101 gccggccagc cgcatgtcgc aacagctggc gatctccatg cctgcgccca tgcaggcgcc
     5161 ctcgatctgc gccacgatgg gcaggtcgca gtcgagcatg gcctgcaggc cgccccagac
     5221 ctcgttttca tggaagcctt gcagggcggc ttcttcaaag cggaaggccg ggtactcggc
     5281 gatgtcgccg cccgcacaga aactggcgcc ctccccgcgc agcagcacgc agcgcacatc
     5341 gccgcgctgc tgcaggccgg tgaacacctc gcgcagctgc tgccacatgg cacgcgacat
     5401 ggcgttgagc ttgcccggat gggacagggt gaccatggcg atggggccgt ccgtggccag
     5461 cagaacctgt cctctcatga atactttgga aaggtcaatc gagtttggcg cctgacgcct
     5521 tcactacctg cgcccatttc ttgacctctg cgctgatgaa gctgccgaac tgcggacccg
     5581 acagcccggt gaactcggca ccctgacgcg cccagacggc cttggcttcg tcggtggtgc
     5641 aggccttgcg gatttcctcg atcgcgcgcg cctgtgcatc ggccggcatg cccttggggc
     5701 cccagacacc gtaccaggtc gtcacgttgt agtcgggcaa gcccatctcg gtggcgctcg
     5761 gcacgtcggg aatggccggg ttgcgcttgt tgcccgacac catcagcgcc ttgatgcggc
     5821 cgccggcaat gtggctggcc gatgagccca ggccgtcgaa catcatttcg acgtcgccgg
     5881 caatcaggcc ctgcagggcc gggcccgcac cgcggtaggg aatgtgggtg atgaaggttt
     5941 tggtctgctg cttgaacagc tcgccggcaa ggtggtggga ggtaccagcg ccggccgagc
     6001 cgtagttgag cttgcccgga tttttgcggg cgttgtccag aaactccttg aagttggcca
     6061 cctgcagctt ttgcgggttg accaccacca cctgcggcac gctggccacc aggatcagcg
     6121 gcaccaggtc tttctcgatg tcgtagtcga gcctggggta catgctgggc gcgatggtgt
     6181 ggtgcaccgc gcccatgaac agggtgtagc cgtcggctgg cgccttggct gcaatgctgg
     6241 cccccagggt gccgccggcg ccgccgcggt tgtcaatgat catctgcttg ccggtcaggc
     6301 gtgcaaactg cgccgacatg gggcgcgcaa acgcatcggt accgccgcct gcgggaaagg
     6361 gaacaatcat ggtcaccggc ttggcaggcc agcccgattg cgcgtggctg cccagggaaa
     6421 acgcggcaac gccggcggct gctgcacgca gcacattgcg cctggacgcg ttgatgtcaa
     6481 agctcgatgt catgtctgtc tcctgtcgtt gttgaaatag ggtttggtta gtgcgcttac
     6541 gaaatataca aatcttcgat gtcgcccgat acggggattt tcccctgtga cagcagcgcc
     6601 ctgtgcttgt ccaggcgttt gaggtcatag aggtagttga ccatcagccc gcaggactgc
     6661 ttgagtccct tggccgacgg atcgcccatc cagttgagcc gctcgatgcg cgcaccgttg
     6721 cccaggtgaa agcgcgccac cgcatcgagc ggcttgccgt cgtcgagctc gcgccccagg
     6781 taatgcgccg cgcaacgcag caacatgcgg cgcaccggcg atttgtcgtc cagggccggc
     6841 gcattgtcgg cagcagtgag gaaatgctgc gcggtggggg gttcaaaccc gatggccctg
     6901 cccagcgcgg tgcgttcctt gtcatcgagc agcgccagca tcgcgccggc atgtttcccc
     6961 agccagtgac ggaaaccggg aatcggcgag agggtcgcaa agtgtttgag ccgcggaaat
     7021 tcggcacgca gggtttccac cacgcgcttg atcagcgagt cgccgaaact cacgccgcgc
     7081 aggcccgcct gcgtgttgct gatggagtag aaaatcgcgg tggtggcctt ggccagatcc
     7141 gccggtgcgg ccgactcgtc gagcagcggt gtaatgccgg cggccatctc gtccatcagg
     7201 gccacttcga caaaaatcag cggctcatca ggtatgcgcg ggtgaaaaaa cccataacag
     7261 cgccggtcgc tgtcgagccg gtttttcaca tcggcccagc tcttgatgtc gtgcaccgcc
     7321 tcgtacttga tcagcttttc gatcagcgat gccggtgagt cccagctgat gcggcgcagg
     7381 tccagaaaac ccacgtcaaa ccaggtcgag aacatgtact ccatctcgac atcgagcgcc
     7441 tgcaggcgcc tgtccgcctt gaggtaaggc agcatgtcgg cacgcatgtc gaccagaaag
     7501 cgtatgcctt ccggaaacgc gctgaagcgc tgcagcaggc gcctgcgcgg cgacacggtg
     7561 gcgcggcggt agcgcacctc ggccacagcc tcgtcgggcg ttcccaccgc ggcggcaaac
     7621 tgggcttgcg ccagtttgac cttctgcgca tcggcgacaa actgctcact catcagcagc
     7681 cacatgtcgc gccgctcgtt cagcgcggcg ccggcatacc agccggccac gcctttggcg
     7741 cggcggccgc cttcgacttc gctgacctgc gggtccacga tggccttgag ctcctccagc
     7801 gtgcggcgca ggacgcgcgg cgacagcgcc tcctcctttt gccgcaaggt ggccttcagg
     7861 cgctcgcggg tggagcgggc cggcgccggt gcctccggcg aagcgccgga cgctaccttt
     7921 ttgggagcat ctgacgcaga caccacggtg gcttcagccg gttttgatgc tacttttttg
     7981 tcacccgaga agcgcgacac attgcggttg agccagtcgg cggcggtcat gaggccaccc
     8041 tcgtgcgctg gtggcgggcc agttcgcgca gggcttcgcg ctggcgcgtc aggtgggtgc
     8101 gcatggccgc gtcggcgcct tcgctgtcgc gggccttgag cgcggcaaat accgccatgt
     8161 gttccgacag actttgcgcc agccggccgg gcgcatgcag ctgctgcgca cgcgccagct
     8221 tgaggatctt gcgcaagtcg gcgattgccg tggcgagcca gcggttgtcg gccagcgtga
     8281 tgatggcttc gtggatcgcg aaattggtgt cgtaatactc gttgatgcgg ccggccctgg
     8341 catggccggc cagccgctcg tgcagcgtgg tcagcgccgc caggtcggcg ttgctcgcat
     8401 tgcgcgcagc ctcgtaggcg cagcgccctt ccagcagggc aatcaccgga aagatatcgt
     8461 ccaggtcctt ctcggtgacc tcgctgataa agcagccgcg ccgcggctcg tggcgcagca
     8521 gcccctccgc ggtcagcacc ttgagcgcct cgcgcagcgg cgtacgggaa atctgcagcc
     8581 gctcgcacag gaccagctcg tccagaaaac tgccgggcgc ataggtaccg gcaaaaatct
     8641 gttcacgcag ggtggcagcc acttcctggt gcaatgaatt ctggacgagt cgcatgatgg
     8701 tatccgttgg tcgtcgtatg gcggaaaaat gcctgggcgg gtctggcggt ggcggtgggc
     8761 atgtgcctgg cttttgccgg ccctctgcgc ctccgcgttc tcattcatcg ctaccgtaat
     8821 tttatgtaat tacacataat tatgacgagc aagcctgtgg agtgcaagcc gcttcagcga
     8881 caaaccaaca ggtgaaaacc cgcggtttcc ggggcctaaa aaagggaggc taccgttatt
     8941 gcaagtggat cgggtcggaa gtaagctatc aaaacgatag ctgctgacgc ccgaccagca
     9001 aggactgagg ccactttcag gctctttttt cagcccacag ccagagccag acaccggccg
     9061 cgatcatcgg caggcacagc cactgaccca tgctcatgcc cagcgccagc acgccgagga
     9121 agtcatccgg ctcgcggaaa aattcggcga tgaaacggaa cacgccgtaa ccgacgagaa
     9181 acgcccccga cacctgcccg gtcttgcgcg gcttgcgcgc atacagccac agcagcacga
     9241 agagcagcag cccttccatc aggaactggt aaacctgcga ggggtggcgc ggcagcatgg
     9301 agccgctgtg tttgaacacc atgccccagg gcaaatcggg gctgctgaag cggccccaca
     9361 actcgccgtt gatgaagttg ccgacccgcc ctgccgccag cccggtgggc acgcagggcg
     9421 caatcaggtc catcacctgc agccagggct tttgccgcgt gcgggcaaac cagacctgcg
     9481 agacaagcac gccgatcagg ccgccatgaa aactcatgcc gccctgccag acgtagaaaa
     9541 tttccagcgg atgggccagg taataaccgg gcttgtagaa caggcagtag ccgatgcgcc
     9601 cgcccaccac cacacccatc acccccagaa acaggatgtc ctcaatgtcc ttgcgtcccc
     9661 aggcggccgc cccggtcatc gacgcataag gttcatgccg caggcggcgc aaaccaagaa
     9721 acaggaacag gccgaaggca gccaggtagg tcaggccata ccagtggatg gccagcggcc
     9781 ccaactgcag ggccacagga tcgatggtgg ggtgaatcag catgcggctt attgtgccag
     9841 cgtgcgcccg cggcagccga tgcggccggg ccgtcagtcc ggcagcgccg gggcaaacgc
     9901 aaccaggcgc tgaaggcagg gattggccgc gtctgcaggc cagaacaggc tggtttcgca
     9961 aaccggcacg cggcgacctt gtttacctgc cacgctgcgg tacaccacac cgggccgctg
    10021 gaactgccgc acactgtcgg gcacccaggc cacgcccagg ccggccgaca cgaggttcac
    10081 gatggtctgc atctggatgg cctcctgcgc cacctgcggc ggctgcgcgc cggcatggta
    10141 catgccgaag atcgcgtcgt acagcgaagg cagaatgcgg cgcggaaaaa tcaccagcgg
    10201 ctgcgccagc acctcgtcga gcgccaggga cgccttcgca gccagcggat gctgttcggg
    10261 cagcgccagc accagcggct cccgggcgac cagccggcgc gtcaggccag gcggcgcaaa
    10321 acccggggaa tgcagcatga ccccggcgtc gatgtcatgg cgttcaaaag cctgcaactg
    10381 catgtcgccg gtggcctcga gcagttccag ctggacctgc ggcgcatgtt cgcggaaagc
    10441 ccgcacccag gcgggcagca gggaaaatcc ggcggtcgac acgaacgcca gccgcaggcg
    10501 gccgacttct ccggccgccg ccgccctcac ctgcccgggc agggcctgcg cgcgcgtcag
    10561 caggtccagc acctgcggca gcagcgccag cgccgccgcc gtcatgtgca cgctgcgttt
    10621 ggtgcgatcg aacaaccgca cgcccagcag cttttccaga tgggcaatcg cctgggtcag
    10681 cggcggctgc gtcatgtgca ggcgcgctgc ggcacggccg aagtgcagct cttcggccac
    10741 agccacaaac tggcgccaaa gccggagttc tatggttgga tcagtcatat gccatacata
    10801 ttaatacagc ctgaaaaata tattggaagc gctggatgag gcggcgcata ctctggcttt
    10861 tgctccacct ttcccatctt ccccaccgca gagacatccc atgacccaac aggacaaaga
    10921 cagcgtcggc gacgccatca accgccgcag caaaaacatc accgaaggca cctcgcgcgc
    10981 accgaaccgt tcgatgtact acgcgatggg ttacgaggcc ggggacttca agaaacccat
    11041 ggtcggtgtc gccaacgggc acagcaccat cacgccctgc aacagcggcc tgcaaaaatt
    11101 ggccgatgcc gcgattgccg gcatcgagga agccggcggc aatgcgcagg tgttcggcac
    11161 gccgaccatc tccgacggca tggccatggg caccgagggc atgaagtact cgctggtctc
    11221 cagggaagtg atttccgact gcatcgagac ctgcgtgcag ggccagtgga tggacggcgt
    11281 gctggtggtc ggcggctgcg acaagaacat gcccggtggc ctgatgggca tgctgcgcgc
    11341 caatgtgccg gccatctatg tgtacggcgg caccattctt ccgggccact acaagggcaa
    11401 ggatctcaac atcgtcagcg tcttcgaggc cgtcggcgaa cacgcggccg gccgcatgag
    11461 cgacgaggac ctgctgcaga tcgagcgccg cgcgattccc ggcaccggca gctgcggcgg
    11521 catgtacacg gccaacacca tgtcgtccgc cttcgaggcg ctgggcatct ccctgcccta
    11581 ctcgtcgacc atggccaacc cgcacgacga aaaggccaac tccgcgaaag agtcggccaa
    11641 ggtgctggtc gaagcgatac gcaagaacat caagccgcgc gacatcgtga ccaaaaagtc
    11701 gattgaaaac gccgtcgccg tgatcatggc caccggcggc tcgaccaatg ccgtgctgca
    11761 tttcctggcg atcgcgcatg cggccggcgt ggactggacg attgacgact tcgagcgcgt
    11821 gcgcgtcaag acaccggtgc tgtgcgacct caagccctcg ggcaaatacc tggcggtgga
    11881 cctgcaccag gccggcggca tcccgcaggt catgaagatg ctgctcaagg cgggcctgct
    11941 gcatggcgac tgcctgacca tcgaaggaaa gacgattgcc gaggtactca aggatgtccc
    12001 ggacgcgccg cgtgccgacc aggacgtgat ccgcccgatc gacaaaccca tgtatgcgca
    12061 gggccacctg gccattctca agggcaatct ttctcccgaa ggctgcgtcg ccaagatcac
    12121 cggcctgaaa aacccggtga tgacgggccc ggcccgcgtg tttgacgatg agcagtcggc
    12181 gctcgaagcc attctggccg gcaagattgt cgccggcgac gtgatggtgt tgcgttacct
    12241 gggccccaaa ggcggccccg gcatgccgga aatgctggca ccgaccggcg cgctgatagg
    12301 cgcgggcctg ggtgaaagcg tgggcctgat caccgacggc cgcttctcag gcggcacctg
    12361 gggcatggtg gtgggccacg tcgcgccgga agcggcggcc ggcggcaaca ttgcctttat
    12421 ccgggaaggc gattccatca ccatcgacgc caaacaattg ctgctgcaac tgaacatcag
    12481 cgacgccgaa ctggccaaac gcaaggaggg ctggaaagcc ccgctgccgc gttacatgcg
    12541 cggcgtgcag gccaagttcg ccttcaacgc ctcgagcgcc agcaagggcg cggtgctcga
    12601 cgactattga aagcgcagca accaacagcc gagcatcaaa aaagccccgg caggcaactg
    12661 caggggcttt ttttgatggc gtgaacggcg ccggagcaca gcgagcggcg gtccccttcc
    12721 agcaggggcc gcgggtccgg ctctgccggc ccgcaggcgc agcgccccct cggggggcag
    12781 agagctacac gcagtgagcg aac
//
LOCUS       DAIDFG010000056        12762 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_56_length_12762_cov_20.517904, whole genome shotgun sequence.
ACCESSION   DAIDFG010000056 DAIDFG010000000
VERSION     DAIDFG010000056.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 12762)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12762)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12762
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56_length_12762_cov_20.517904"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2088 a         4236 c         4020 g         2418 t
ORIGIN      
        1 cccagcgtgt gctgctggcc ctcggccacc tggtcgaggc tggccatcca gctggttttc
       61 ggtgcatagc gatgcgggtt ggcgacgcaa tggtcgatcg cctcgcgcca gacccgcgtc
      121 acctgcgcta cataagcgag gctgcgctgg cgcccctcga tcttgatggc gcgcacaccc
      181 atcttcatga gctggggcag aagttcgagc gtgttcaggc tggcgggctc ctcgatggcg
      241 taataactct cgtcatcacc cacatcgaag cgccccttgc aaagcgtggg gtagccggca
      301 ttttccccgc tggcataacg gtcgatcaac acaccgccca gacgcgattc cagaccctgc
      361 ggtgtttcct gccaacgcac ggacttggcc ggcgaacaca cgccgtgtgt attgggcgac
      421 tcgcccgtca cataggacga cagcgcgcag cgcccctcca ccatcacaca caggctgccg
      481 aaaccgaaca cctcgatttc caccggcgtc ttgtcgatga cctgccgcac ctgttccagc
      541 gacaacacac gcggcagcac cgcgcgaacg acgccgaact gctcgcggta gaagttgatc
      601 gcctcgtaat tggtggccga accctgtacc gacaggtgca ggcgcagcgc cggataacgg
      661 gaagcggcgt actgcatgag cccggggtcg gcgaggatca cggcatccac ccccaagtcc
      721 acagcctggt cgagcgccgc gcgccaggga ccggggttgg cggcctgcgg ataggtgttg
      781 agcgccatga agaccttgca gccgcgctcg tgggcataac ggatgcccgc ggcaatggcg
      841 gcctcgtcga aattgaggcc ggcaaagttg cgtgcgttgg ttgcgtcgcg caggcccagg
      901 tagacacagc tggcgccgtt gtccaccgcg gccttcaggg ccggcaggct gccggccggg
      961 cagaccagtt ccggaggggc tgaaggggac agggcaggtt cggcaagagg tgtagaggtg
     1021 gtcatgcggg tggcgatcgg acgtcgggcc ccgaacggag ccgtcacagc gcacgaccat
     1081 acgccgatcc atccggatac tttctgatcc ccgtcaagaa atgggctcaa ggacctcggg
     1141 gccgatcgtt gcaagtgggc tctccaacgc ctgaccttcc ggcgtgaagc agagtcgact
     1201 gccagagcaa ggcatggggg gcgtcatcaa ggcaattgac ctggatcaat aggggcattg
     1261 tcgtctggcg ttcttataga tgtattatat atacatctta taaatcatcc ttgtccttgt
     1321 tattgaatgg agtctccatg agccatgcct ctgcccccgc cgcgctcgat atccgcgccc
     1381 ttgcgccccg cgagcgacat cccctgatct tctcgacctt cagccaactc cgcgctggcc
     1441 aggccatgga attgatcaac gaccatgacc cgcagccgct gcatcaccag ttccaggccg
     1501 aatggccggg caagttcagc tgggcttacc tggaacaggg accggcgacc tggcgcgtgg
     1561 ccatcacccg actgtcatcc gctcacagcg acggccaatg ctgcggctcc tgcggcggcg
     1621 cctgaccccc ctttaaagaa accagaagga attcgcgatg aaaaacaaca acatgctgtg
     1681 gcgctggctc ggcttcatct ttgtcctgtc gttcggagca ctgggttacc tcggcgtgca
     1741 gatctacctg acagccccgc ccatccccaa ggccgtcgtc accacgtcgg gcgaggttct
     1801 tttcaccggc gagcaggtcc agaatggcca gcaggtctgg ctggccagcg gcggccagca
     1861 gcaaggcagt gtctggggcc acggcgccta tctggcgccg gactggtcgg ccgactggct
     1921 gcaccgggaa gcaacggcgc tccagaccat ccatacgcag gccttgcgca aggatccctc
     1981 ggccatcaca gcatccgacc gcggcgcggt cgatgctgcg ctgaaggagg aaatgcggcg
     2041 caacacctat gaccccacca gcgaaaccgt caccgtgtca cgcgaccgtg cacaggcgat
     2101 ccgcgaagtg gcgagtcact acaacgccct gttcggcagt gacgtgcagc tggacaaact
     2161 gcgtgaccag tacgcgatga caggcaacct gctgccggat gcggcggatc gccaggccct
     2221 cgcagccttc ttcttctgga cgtcctgggc cgcgaccacc gaccggcccg gcgaggaaaa
     2281 cctgtcttac accagcaact ggccgcatga gcccctggtg ggcaacaaga tgaccgactc
     2341 ggccgcgatc tggtcgatgg tcagcatcgt cttgctgctc ggtgccatcg ccgccatgtt
     2401 gtggctgcac ggcagccagc ggcatgagcc ggaggccctg ccgcccaagg ccgacccttt
     2461 gctgggcgcg gtcgccaccc cctcgatgaa ggccacgcgc aagtacttct tcgccgtggt
     2521 cggtctcatg ctgctgcaga tcgccatggg cgtggtcacg gcgcactacg ccgtcgaagg
     2581 ccagtccttc ttcggcctgc cgctggccca ggtgttgcct tatgtggtga gccggacggt
     2641 gcatacccaa gtcggcatct tctggatcgc caccgcctgg ctggccaccg gcctgtacat
     2701 cgcccccctg ctgtcgggcc gcgaacccca gctgcaaaag ctcggcgtgg acgttctgtt
     2761 ctgggcgctg attgccatcg tggtcggctc caccgtgacc ggctggctgg gcacgctgca
     2821 gcaccgcggt gtggacttca gcttctgggt cggaaaccag gggctggaat tcaccagcat
     2881 gggccgtgtc tggcaaatcc tgctgttcgt gggtctgctg ttctgggtgt ttctgctcgg
     2941 ccgcgccctc tggccggcgc tgaagaaacc ttcggaaacc cgcggcctga tcgccatggt
     3001 cttcctgtcg gccacctgca tcggcggctt ctacgcgaca tcgctgacct ggggccagaa
     3061 cacgcactac tcgatggtcg agtactggcg ctggtggctg gtccacctgt gggtggaagg
     3121 tttcttcgag gtgttcgcca ccgccgtggt cgcgctgatc ttcaccaagc tgggactggt
     3181 tcgcactgaa agtgccaacc gcgccatcat cgccgagacc atcgtgttcc tgttcggcgg
     3241 catcctgggc acgctgcatc acctctattt caccggtaca cccacctcgg tgatcgccgt
     3301 cggtgcggtg ttctctgcgc tggaagtcgt gcccctgacg ctgatcggcc tggaagccct
     3361 gcagacctac cggcgttcca aagccctgcc ctggctggct gcctacaaat ggcctgtcat
     3421 gtgttttgtc gcggtcggtt tctggaacac cgtcggtgcc ggcctgctcg gctttgccat
     3481 caacccgccg gcctcgctct actatgtgca gggcctgaac atgaccgcag cccatggtca
     3541 cgccgccctg ttcggtgtct acggcatgct cggcatcggc ctgatgctgt tctgcctgcg
     3601 cggcctgtat gcgcgccacc tccatgccga caacatcctg aagcctgcct tctggagcct
     3661 gaacatcggc ttggcgatga tggtcttcct gtccctgctg ccagccggca tttaccaggc
     3721 ttgggccagc gtgagcaaag gcctgtggta cgcccgctcg ccggaaatca tccactcgcc
     3781 cgtgatggaa acgctggtct ggatgcgggt acccggcgac atcctgttcg ccatcggggc
     3841 ggccctgctg gcctggtatg ccctgcgcct gttgcggcgc cccgcggttc aacccgcgag
     3901 cgccctcacc gccaaggcgg tggccggcct ctgacctccc tcgcaaggcc ggccgcgtcg
     3961 gccttgcgtc cctttccccg attttttatt ggagttttca tcatgagcga gatccagccc
     4021 ctccacaacg tccacccctt cgacgcccgc ggtgttgcca aacgcttccg ccacgcggcc
     4081 atcttcggcg ccctggaaag cctgaacccc ggggaaacca tgcgtttcgt caacgaccac
     4141 aaccccctgc cgctgctggg ccaactgcag caggccttcg gcgaaaagct gggcatcacc
     4201 tatgtgcggc gcgaaccggg ggaaatcgtg atcgacttcc aggtacgcgg ctgaggcctt
     4261 acattcaccc catgaaactc accgccttta ccgactacag cttgcgtgtg ctgatctacc
     4321 tggccgcgca gccccaaaaa cgcgccacga ttgccgaaat cgcgacctca ttcgccgtgt
     4381 cggaaaatca cttgaccaag gtggtgcacc tgctcggcaa aaacggctgg ctggccaatg
     4441 tacgcggcaa gggcggcggg ctcgaactgg ccatgccgcc cgaactggtc ggcgtcggcg
     4501 cggtggtgcg ccagaccgag ggcgcggcgg tggtagccga atgtttttct gatgaggcca
     4561 accattgcgc gatcacacgc atctgccggc tgcgcggtgt actcggtgag gcggtcgagg
     4621 ccttctacac cgtgctggac cgctacaccc tggccgacct ggtccagaac cgccaggcgc
     4681 tggccaaggt gctgtttctc gacagaactc cgcccacgcc ggcggcaaaa cacgccgcat
     4741 gagtgctgat gccggagctg ccatgactga gcccagagcg ccattcccct atgaaggccc
     4801 tgaggcgctg cgccagcccc tggtggcggc actgacgcgg gtggtcgatc cggaggttgc
     4861 catgagcatc gtcgatgtcg ggctcatcta tggcgtcacc gtgagcgccg agaaactgca
     4921 tgtccggctg accatgacat ccgccgcgtg cccggtcgcc gatctcatca tcgacgaggt
     4981 cgaaaccgag ctggaccgcg tggcaccgcc ggaattgctg attgaagtgg aactggtgtg
     5041 ggaaccgccc tggtccgccg accggatgag cgagcgcgcc aggcgcttca tgcagtggtg
     5101 aaaacatcgc accgacccag gcccccgcaa cgccagacgg caccgcggtg gatcaccctg
     5161 gccgtgcggc cactgctgct ggcaggggtc gctctgtcgc tgctggccgg catcacaggc
     5221 ggcctgctgc gcgctggtgt ggcccttccc gccctgccgg atgccacctg gcccggccag
     5281 gcggcgctgg cccatgcggc tttgatgatc tgcggttttc tcggcaccgt catcggcatc
     5341 gaacgggccg tggccgtcaa gctgcgcgcc gctttccttg cgccgctggc ctcgggtgca
     5401 ggtggcgcgc tcctgctgct gggtcaaccc gggccgggcg cgtggctgat cgtggccgca
     5461 gccgccatct tcaccgcggt caacatcgtg atcgtgcggc gccagcgcgc cgcccacacc
     5521 tggctgctgc tggtcgctgc aggcgcgtgg ctggccggca acctgctgtt cgccacgggc
     5581 caaggcagcg ttgcgacgct gccctggtgg ttcgcctttc tcgtgatgac catcgccgcc
     5641 gagcgcctgg aaatgacgcg gctgatgcgc cggcgacccg ccgcagaact gtcgctgcag
     5701 gccgtgctgg cggcgctgct cgcgggcgcg gcctggtccg ccctggcacc ggtcgccggc
     5761 gggctgttgt acggcacagc gctgaccctg ctggcgctgt ggctggcggt cttcgacatt
     5821 gcccgtcaca ccgtaagggc gcacggactg agccgttaca tggcggtttg cctgctaggc
     5881 ggctacgcct ggctggccgt ggcgggcgtt gcgtgggctg ccaccgccct gggcctgccc
     5941 ctgcgcgacg ccgcactgca cgccctcggc ctgggcttca tcgtcagcat gatgatgggc
     6001 catgcaccgg tcatcctgcc ggccattgca cgcgtcaagc tgcagttcgg cgcctttttc
     6061 tacgtgccgc tggccgtgtt gcacctctcg cttctgctgc gcctggtcgc tggaatggca
     6121 ggtgacccgc tgcacagcac cggtgccctc ctcaatgctg ccgcaatcgc gctgtttgcg
     6181 gccaccatcg ccggcgctgc cgtcgcctgg cgcatccagc acggcgggcc cgacgccccg
     6241 cccacccgaa agacatcctc atgacaccat tgagcaaaat cgcagaacct ccccgccctt
     6301 caccagcacc tgccggctgg gccctctggg acctcggctt tcgtccgttt tacctgctgg
     6361 ccagcacctt cgccgccttg tccatcggcc tgtgggcgct gcaattcacc ggctggctgg
     6421 gcctgcccta cctgcagggg ccgatgtggc atgcccacga gatgctgttc ggcttcgccc
     6481 tggccgtggt cgttggtttc ctgttcacgg ccggtcgcaa ctggtcgaac cggccgacgc
     6541 ccaccggctg gccgctggcc gcgctggcgg ccctgtgggt ggcgggccgg gtgttggtcc
     6601 tcacgccatt cggctgggcg gcgctacttg ccaatgtcgc tttcccgctt gcagccgcca
     6661 tcgcgctggc gattcccttc atcgccgcgc gcaatcgccg caactacttt tttgtcggct
     6721 tgctgctgct gatgtcggca gccgtgctcg gcattcacct ggcacaactc ggcgtcgtgc
     6781 agcttccggg ctgggtcggc atccagctcg ccctcgacgt catgctgttc atcatggccg
     6841 tgatgggcgg ccgtgtgatt cccatgttca ccaacaacgg cgtcccgggc gcccatgcca
     6901 cacgtcagcc cctgctggag aaagccgcgc tgggcagcgt gctggtgctg ctgctggccg
     6961 acgccgtgca actgcagggc gcgtggctgg ccgccgtggc gggcgcatgc gcgatggccc
     7021 accttgcccg ctggagcctg tggcgcccct ggaagaccgg gcgtgcgcca ctggtctggg
     7081 tgttgcaggt cgcctacttc tggatcccgg tgcatctcgt gctgcgcgcc ctggctgcag
     7141 cgggatgggt aaccccttcg ctggccacac acgcgctgac ggtgggcgcc atcggcggcc
     7201 tcattatcgg catgatgacg cgtaccgctc gcggccacac cggccggccg ttacgggccg
     7261 accgcagcga tgtggcctgt tatgtccttg tcctgttcgc ggcgctggtc cgcgtgttca
     7321 tgccgctcgc tgtcccggcc tggatcatcg aggccacgct gttgtcggcc gcactgtggt
     7381 cggccggctt tgcgctctat gccgtgcgct actggccggt gctgacgcga ccgcgtattg
     7441 acgggcaggc aggctgactg caggcgcttt acccgcgggg cgcaaaatgg ggtcatgaaa
     7501 ccacccaagc gcctgcagtc attgatcgaa gaaggcctga tcgacaccgt ggtgcggcag
     7561 ttgatgagcg gcaaggaggc catggtctat gtcgtgcgct gcggcgacga tacgcgctgc
     7621 gccaagattt acaaggaagc cgaccagcgc agcttccgcc aggcggtgga ctataccgaa
     7681 aaccgcaagg tcaagaacac gcgccaggcg cgtgcaatgg ccaaggggac ccgctttggc
     7741 cggcaggcag ccgaggcggc ctggcaaagc gccgaagtcg atgcacttta ccgcctggcc
     7801 gccgccggtg ttcgcgttcc caagccgcac aacttctgcg acggggtact gctgatggaa
     7861 ctggtgaccg acgaacatgg cgatgcggcg ccgcgcctga atgatgtgga attcacgccc
     7921 gaagaagcgc gcctgcacca tgccacactg ctcacggaag tggtgcgcat gttgtgcgcc
     7981 ggcgtcgtgc acggcgacct gtccgagttc aatgtgctgc tggccgcgga cggcccggtc
     8041 atcatcgacc tgccgcaggc ggtcgatgcc gcaggcaaca accacgccag ccgcatgctg
     8101 ctgcgcgatg tcgccaacct gcgcggcttt ttcggccgtt tcgcgcctga cctgctgacc
     8161 accgactacg gccatgagat ctgggacctg taccagcgcg gcgtgttgcg gcccgatacg
     8221 gtgctcaccg gtcgtttcgc gcacaaacgc gggccggtgg acatgggcgc cgtgatgggc
     8281 gaaatcgatg atgcgcgggc cgaagaagcg gcgcgccgcc tgcgcatgca gaccctgtag
     8341 ggacacgcgg cctggtgccc gacagacaga cgcacttctt tcgtgtacgg tggtccggaa
     8401 ggctgcacat cccgtgcatg cgaaggctcc gatgaacaat ccccactaca caccccgcac
     8461 ctttggcgac cgctttgcct acgcctttgt ccgcttcctg cgcttttttg ccgacctgtt
     8521 ttttgccaag cgctacggcc accgggcgat cgtgctggaa accgtcgccg cggttcccgg
     8581 catggtgggg gccacgctga cacacctgcg ctgcctgcgc cgcattgaag acgacaaggg
     8641 ctggatacgc aagctgatgg atgaggccga gaacgagcgc atgcacctga tgacgttcat
     8701 caaaatcgca cagcccaacg ggttcgagcg cgcactggtc atccttgtgc agggcttttt
     8761 ctacaacgcc tacttcctgc tgtacctgat cgccccgggc gtggcgcacc gggtggtggg
     8821 ctactttgag gaggaggcct acaccagcta cacccactac ctcgaactca tcgactcggg
     8881 cgcccatgcc aatgtggccg cccctcagat tgcgaaggac tactggaagc tgcccgccga
     8941 cgcaaccctg cgggatgtgg tgctggccgt gcgggaagac gaagcggggc accgcgatgt
     9001 gaaccacggc ttcgccaatg acctgcatgg gggcggcgcg cagcagcacg ccgccagctg
     9061 acaatggttc tccgggcgtg tttcagcgca ccgggttttc caggctgccg atcccgtcga
     9121 tctccacccg caccacatca ccggcctgca ggtacttcgg cggtttgaaa ccaatcccga
     9181 caccgaccgg cgtgccggtg gcgatcacgt caccgggata gagcgtgatg ccggccgaga
     9241 gggtttcgat cagtttggga atgtcgaaaa tcaggtccac ggtcgaggcg ttctgacgtt
     9301 cctcgccatt gacgtaacaa cgcaccctgg tcttggtgcc gtcgagttcg tcggcgctca
     9361 ccagccacgg gcccatggga cagaaggtgt cgaacgattt acccatgtgc cattgctggt
     9421 gacggctttg ccagtcacgc gcagtgatgt cgttgacgat ggtgtagccc cagacatggg
     9481 ccatcgcgtc tttcgctgca atgcccttgc cgccacgtcc gatgatgacg gccagttcgg
     9541 cctcgtaatc aatcgcggtg gaaaccgccg gcatctcaat ggcggcgccg ggaccgacca
     9601 cggactcggg caccttggtg aagatgatgg ggtccgcagg aatgtcgccg cccgatttgg
     9661 cgctgctgtc aaagccgctg ccggcaaact ccttcgcgtg cgcaaagtag ttttttccga
     9721 cgcagaaaat gttgcggcgc ggcttgggca gcggggcgat gatctgcact tcactgaggg
     9781 cgatggccgg cagcagggcc ggcaaggtgt cgccgcgcgc ctgcatctcg atcagcgcct
     9841 gcgcccccag gtcgctctgc gcttcgggca gatcaaaggg ctggacctgc tggaggtcgg
     9901 cggacaccag gcccacaccg gagcggccct tcaggcggta ggcaacaatt ttcatggcgc
     9961 tgtctctctt tgtttttgcc cggcaggaaa gcgccggggc ggttgtggaa gcaggagcct
    10021 ggagacagac tcctagggat tgcgataacc ccaaaatttc aggcgaatgt tagcatgcaa
    10081 accataaaaa gtgcgccggt ttttcgactg ccgccgacca accctcagga gacaaacatg
    10141 aaacgcagat ccgtattgct gggtgccttg ctcatttcag cgaccacctg ggtggccgca
    10201 caaaacgcgt ggcctgcgcg ccccatcacg atgatcgtgc ccttcccgcc cggcggcctg
    10261 gccgacctcg tcgcgcgccc tgtggccgaa gccatgtcgc gcaacctggg tcaaccggtg
    10321 gtgattgaaa acaaggccgg cgccggtggc ggcatcggca tgggcgtggc tgccaaagca
    10381 aaacccgatg gctacaccgt gcttctggcc ctgtcctcgc tgaccgtcat tcccgaggcg
    10441 gacaccctgc gggggcgccc gccgatgttt gcgctgagcg acctgcgtcc catcgcacgg
    10501 tttaccgccg acccgaccgt gctcgcggtg cgcgccgaat cgccgtggaa aaccgtgaag
    10561 gattttgtcg aggatgccag gaagcgcccg ggcgcgatca actacggctc atcgggcaac
    10621 tacggcacca tgcatgtgcc catggaaatc ctctcgcaga acgccggcgt gaaaatgacg
    10681 cacatcccct tcaccggagc aggccccgcg gtggtggccc tgctcggcgg gcaaatcgac
    10741 gcggtgtcct ccgggcccgc aaccgtgctg cagcacgtca aggccggcaa gttgcgggta
    10801 ctggcccatt ggggcaatgg caagctcgct gccctgccgg aggcgcccag cctgaaagac
    10861 gcgggttaca acgccgaata cgcgcagtgg tccggcctgt tcattcccaa agacacaccc
    10921 gaacccattg cccagcgcct gcgcgccgcc gccaacgctg ccgcgcagga cagcaaggtc
    10981 aaggacgtca tcctcaacgc cggcagcccg gtgctgtacc aggacgctgc cgatttcgag
    11041 aactacgtgc aggccgacgc caaacgcatg gccgatgtcg tcaagcgcat cggcaagctg
    11101 gattagggcc tgatcaccct atttatgcaa gatgcgttgc gagtgtgaac aggccctagg
    11161 tctttccgtc gcccggtttg aacgccccaa taaaaaaggg ccccgcgggg cccttttttt
    11221 gttgccggcg ccgataaaaa ctcacaccag caaggcgttc acccgcttca cgtaggccga
    11281 aggatcatcc ggcaacccgc cttcggccag caaggcctgg tcgaacagga tatgggcgag
    11341 atcgtcgaag tggtcgctgc cctccagctt tttgaccagc gggtgctgtg cattgatttc
    11401 cagaatcggc ttgacctcgg gcgcggcctg gccggcctgt ttgagcatgc gggcgagctg
    11461 cgtggacatg tcgccttcct gcaccaccag acaggccggt gaatcaacca gccgtgtggt
    11521 agcgcgcacg tctgaggcct tgtccttcaa ggcttccttg agtctgtcga gcatgggctt
    11581 gaactgggtc tgcgcttcct cggcggcctt tttctcgtcc tcgtcctgca gcttgcccag
    11641 atcgaccgcg cccttggcca ccgactgcag cggcgtgccg tcgaactcgt tgaggtagtt
    11701 cagcgcccat tcgtcgacgc ggtcggtcat gagcagaacc tcgatgccct tcttgcggaa
    11761 tatctcgagc tgcgggctgt tcttgccggc ggcctgggtg tcggccgtga tgtagtagat
    11821 cgcgtcctgg ccttccttca tgcgcgcctt gtagtcggca aaaccgacac tgacggtgtc
    11881 ggtggtggag ctggcaaaac gcagcagctt ggccaggcgc tcgcggttgg cgaagtcttc
    11941 gcccaggcct tccttgagca ccgcgccgaa ctcggcgtaa aagcttgcaa acttatccgg
    12001 ctcgttggtg gccaggtcct cgatcatgga caacacacgt ttggtgcagc cttcgcggat
    12061 ggccttgacg gcgcggcttt cctgcagcag ctcgcgcgag acgttgagcg gcaggtccga
    12121 cgaatcgacc acccctttga caaaacgcag gtaggtcggc agcagtgcct gggcgtcgtc
    12181 catgatgaag acgcgcttga cgtagagctt gacgccggcg gccttgtcgc ggttgaacaa
    12241 atccatcggc gccttggcgg ggatgaagag cagctgcgtg tattcggtcg cgccttcgac
    12301 gcggttgtgc gtgctggcca gcggggcctg gctgtcgtag ctgatctgtt tgtagaactc
    12361 gtcgtactgc tcgggcgtga tgtctttctt ggcgcgcgtc cacagggccg cagcctggtt
    12421 cacggtttcc cactcgtcgc tggtcaccat ttcgccgggc tggtcgttct cgccttcctt
    12481 ccattcttcc ttccgcatca ggatgggcag ggagatatgg tcggagtact tgctgatgat
    12541 gcttttaagt ttccaggtgt tgaggtactc gctggcgtcg tccttgaggt gcaggatgac
    12601 gctggtgccg cgctctgcac gctctatcgt ctcgacctca aagtcgccgg tgccggcgct
    12661 ggtccagcgc acgccttcgg ccgccggcag gccagcgcgg cgactctcga cggtgatctt
    12721 gtcggccacg atgaagcccg aataaaaacc gacgccgaac tg
//
LOCUS       DAIDFG010000057        12523 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_57_length_12574_cov_17.061507, whole genome shotgun sequence.
ACCESSION   DAIDFG010000057 DAIDFG010000000
VERSION     DAIDFG010000057.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 12523)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12523)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12523
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57_length_12574_cov_17.061507"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2336 a         3897 c         4062 g         2228 t
ORIGIN      
        1 tgcaggtgcc agatgtcgaa ctgacgatcg ttctcgcgcg cgatcaggca gttgtgctgc
       61 gccagttccg ccggcgtggc gggtttgccg tgctggcgga cataggccgg ggcggccacg
      121 accacgcgct ggttgctggc cagcttgcgt gccgtccact cgctcgcgcg ttgcggccgg
      181 ggtgaccaga gccagacggc accgtcaaaa ccttgggcgg ccaggtcggg cagttgttca
      241 taaagctgca gctggatctg gatggccggg tgcctggcct ggaattcggc cagcaccggc
      301 cccagccaga tgcggccgaa gccgaaggtc gccgccaggc ggatcagccc cgaggcttcc
      361 gacttgcgtt cctgcaggga ctgctccagc gcatcgaact ggtccagcat caggcgcgca
      421 cgttcgcaga gcaactcgcc ttcgagggtg ggggccaccc ggcgagtggt gcgctgaaac
      481 agcagcaggc cgagctcggc ctccagttgg gccaggcgtt tggtcacggc ggagggcgcc
      541 atctgcagcg cctcggctgt ggccacgagg ctgccgtgct gttgaatcgc caccaccagg
      601 gccaggtcgg cacgttccat aatttccatt ttgagaataa tcaattcttg gattatttct
      661 tgattcaagg tggtctggca atcacaatcg ctgcatgttt gaagcattcg agaccttcga
      721 aaccagccgc ggggcggtcg ccctgcatgg ccgcaagggc gggctgggcg cgccgctgtt
      781 gctgctgcat ggccacccgc aaacccacgt gatgtggcac gcggtggccc ccgcgctggc
      841 caggcacttc accgtggtca tgatggacct gcgcggttat ggcgattccg gccggcccgc
      901 agcggatccc gggcacctgg cttattcgaa gcgcgaaatg gcgcgggacg cgctggcggt
      961 gatgcagcac cacgggttcg acactttcca ggtgctggcc cacgaccgcg gtgcgcgcgt
     1021 ggcgcaccgg ctggccgccg accaccccgc ggccgtgcag cgcctgatgc tgctcgacat
     1081 cgcgcccacg ctggccatgt acgagaacac ctcggatgcg tttgcgcgcg cctactggca
     1141 ctggttcttc ctgatccagc cgccgcccct gcccgaagcc ctgattgaat ccggcccggt
     1201 gcgttacctg cgcagcgtga tgggcaagcg ccatgccggc ctggccgctt ttgcgccggc
     1261 cgcgctggcc gagtacgagc gctgcatcca gattcccggc acggccgaga gcatttgcgc
     1321 ggactaccgc gcgtctgcca cggtggatct ggcccatgac cgtgccgatg tggccgcggc
     1381 aaagcggctg acgcagccgc tgcatgtcct ctggggcgag catggcgcgg tgggccagtg
     1441 tttcgacgtg atgcgcctct ggcgcgatcg cgccgctgac gtctcgggcc tgagcctgcc
     1501 ctgtgggcat tacattgcgg aagaggcgcc ggcattgctg ctggcgcaag cccagaattt
     1561 tttcaaaccc tgaactccaa ggaaaacaaa catgagcaag accaccggaa agaaaagaat
     1621 tgcagtcatc gctggcgacg gcattggcaa ggaagtgatg cctgaaggca ttcgcgtgat
     1681 ggaggcggct gcggtcaagt tcggcatagc cctgcagttt gatcatttcg atttttcgag
     1741 ctgggactac ttcgagaaac acggcaagat gctgcccgac gactggaaag accagatcgg
     1801 cggacatgat gcgatttatt tcggcgcggt gggctggccc gacaagattg ccgaccacgt
     1861 gtccctgtgg ggatcgctgc tgctgtttcg ccgcgagttc gatcagtaca tcaacctgcg
     1921 gcccgcgcgc ctgatgcccg gcatcatcgc tcccgtcgtg cggcgcgatg gctcggcccg
     1981 cgcgcccggt gaaattgatt tttacatcgt gcgtgaaaac accgaaggcg agtactcgag
     2041 cattggcggc aaggcttttc ccggaacgga ccgcgagttt gtgctgcagg agtcggtgtt
     2101 cacgcgcatc ggcgtggacc gcgtgctcaa gttcgccttc gaactggcgc aatcgcgtcc
     2161 gaagaaacac ctgaccagcg cgaccaagtc caacggcatc tccatttcca tgccttactg
     2221 ggatgaacgt gtcgtcgaaa tggccaaggg gtatccgggc atcaagctcg acaagttcca
     2281 catcgacatc ctgacggcgc attttgtcca gcgtcctgat ttctttgatg tggtggttgc
     2341 cagcaatctg ttcggcgaca tcctgagcga tctcggccct gcgtgcaccg gcaccatcgg
     2401 catcgccccc agcgccaacc tcaatcccga gcggaagttt ccttcgctgt tcgaaccggt
     2461 gcatggctcg gcgccggaca ttgcgggccg tgccatcgca aacccgatcg ggcagatctg
     2521 gtgcggtgcc atgatgctgg agttcctggg gcacaaggat gcgcatgatg cggtgcttgc
     2581 cgcgattgaa aaagtcctgg ctccccacag tggtgcgccg cgcacgcccg acctgggagg
     2641 cacggccagc acggccgatg tgggcaaggc cattgcggcg gcgctggctt gaagcgcgcg
     2701 gtgcagtctg cggcagactg cccctcaaat gcagggtgtg cttacgaata gggcatgaaa
     2761 gcgtgaaaaa taaggctaga atccgccacc gcacgttgta aaaatctgat gttgtattga
     2821 cacgatggga gtccatggct ctaattggaa tcagcagacg atctgcacat gccgcctccc
     2881 gctgccatct ttgtgacagg tgtccagggg agatatccaa gagacaacca gattaacttt
     2941 caacaacgag gggctccttg tgaacaagac tgaactgatc gagcacattg ccaagaacgc
     3001 tgacatttcc aaagctgccg ccacgcgcgc gctggagtca accattggcg ctgtcaaatc
     3061 aaccctgaaa aaaggaggct ccgtgtctct ggtgggtttt ggcactttcg cagtgggcaa
     3121 acgtgctgcc cgcaccggcc gcaacccgcg taccggggat gcgattaaaa tcaaggcagc
     3181 caaggtgccc aagttccgtc ctggcaaagc gctcaaggac gctctgaact gatgggcatg
     3241 aagtaaaagt tgaggtgggg tgcttagctc agttggtaga gcagcgccct tacacggcgt
     3301 aggtcggcgg ttcgagcccg tcagcaccca ccaccgactg gacaaaaagg cgaacagatg
     3361 ttcgcctttt ttattgatgg cttgacgcaa gttttaaatg gccgattgac agccgcacag
     3421 gaaattcgag atgtttgatt tcattcgcaa gcacaccaag gtcacgatgg cattgctgtt
     3481 tttgctgatt gttccgtcct tcatattggt cggcatgaac aacaacggtc ccaccgctgg
     3541 cgctggcggc gttgttgccg tcgtcgatgg caaggacatc acgcagcccg aatgggatgc
     3601 ggcgcaccag aacgaagtga gccgcctcag cgcatcgatg ccgacgctcg atgtcaagct
     3661 gctggactcg cccgaagcgc gttacggcac gctggaacgc ctggtgcgcg atcgcgtgct
     3721 ggcggccgcg gcggcgaaga gcggcctggt ggttgcggac cagaaattgc agaggctgta
     3781 tgccgaggac aaggccatcg ccgccttcag gctgccggac gggaagtttg accgcgcggg
     3841 cttcatcgca caaacgggca tggcgccgga gcagtacgaa gcgggcctgc gcgccgacct
     3901 ggcctcgcgg caggtcatgc tgggtgtctc cggcacgggc tttgcctcga aggccgtggc
     3961 ggacgtttca ctcaacgctt ttttcgagaa gcgcgaggcg caggtggcac gtttcaacac
     4021 ggccgactat gcggccaagg tgaaccccag cgatgtagac ctggagcagt tctacaagga
     4081 caaccaggct ttgtttcagg cgcccgaaga agccaacatc gaatacgtgg tgctggatct
     4141 cgacgccgtg aaaaaaggca tcacggtcaa tgaagtcgac ctgaagactt atttcgaaca
     4201 gaacgcgccg caactcgctg gcactgaaga gcgccgcgcc agtcacatcc tggtcgaggc
     4261 gccagcctcc gcacccgcgg ctgaccgcga aaaagccaag gcgaaagcag agcagctgct
     4321 cgcggccgcc aggaaagcgc cggacacgtt cgccgagctg gcccgcaaaa actcgcagga
     4381 caccggttct gccgcagccg gcggcgacct ggacttcttc gcgcgcaatg ccatggtcaa
     4441 gcctttcgcc gatgccgcgt ttgcaatgaa gaagggcgac atcagcgagg tggtggaaac
     4501 cgagttcggc taccacatca tcaaactgac cgacgtcaag gtccccaaac agaagaccta
     4561 cgaagagatg aaacccgcgc tggaagcgga actcaggcag cagcaggcgc agaagaaatt
     4621 tgccgagacg gcagaaacct tcagcaacgc ggtgtacgag cagtctgaca ccctgcgccc
     4681 gatcgccgag cgcctcaagc tcgaggtcaa gaccgcggcc aagctgacgc gtaccccggt
     4741 ggcgggcgcg accggcgtgc tggccaaccc gaagttcctg gcggccctgt tctcgcccga
     4801 ttcggtggaa aaaaagcgca acaccgaagc cctggaagcc ggccccagcc agctggtgtc
     4861 tggccgcatc acccagtaca ccccggcccg gacgctgccg attgcagagg tcaaggaccg
     4921 cgtgcgcgcc cgcctgcagc agcagcgcgg cgccgagatg gcccgcaagg acggtgccga
     4981 aaaacttgcg gcctggaaag ccagccctgc agccgccact ttccctgcag cggtggtgat
     5041 ctcgcgcgac cagacgcagc agttgcctgc ggcagttgtc agcgccgcac tcagcgcaga
     5101 cgcaggtgct gcgcccgcct tggtgggcgt ggacctgggc gcgcagggct acgccattgt
     5161 ccgggtgacc aaggtactgc cgcgcccggc tcaggccgcc gacatcgcca agcaggacca
     5221 ggcccagtac acccagtggt ggacttccgc ggaaagcctg gcgtactaca acatcctgaa
     5281 agagcgtttc aaggccgaca tcaaggtggc caggccggcc gaacagaaag cgggcgcata
     5341 aagcagagcg tgcggcgcat aaggcgcgca gttcccgcta taatgtgagg cttcggtggc
     5401 tgtagctcag ttggtagagt ccaggattgt gattcctgtt gtcgtgggtt cgagccccat
     5461 cagccacccc agtatttcaa gaaaaaagcc cctttgcagg ggcttttttc tttgggcgaa
     5521 acgcaagcgt ggttcaggcg ctcaaggggg ccagaccaga caggcgccag tattccctga
     5581 cttcctcgag cctggcttca aagtcgcgaa aatcgagcgg cttgctgacg tagctgttga
     5641 gtccgccttt cagggccaga atcgcttcgg actgctcgct cgccgaggtc agcatgacca
     5701 ccgggacaaa aaaagtacgg ggatcttcac gcatgctctg cagcacctgc aggccgttca
     5761 gtttgggcag gccgaggtcc agcaggacga gttcgggctg ttcgtcgggg ttgcggtcgc
     5821 tgtaggggcc ctggcagaac aggtagtcca gggcttcctg accgtcgcgg gccaccacga
     5881 tgtggtggga tgccccgttt tcctcgagga tggtcagggt cagttcaagg tgatcgggat
     5941 tgtcttccac caccagcaag gttggcatgc ccattcgctc ccccgattgc taaggaactg
     6001 tcaatatacc cccaaagtac gcgatgtccg gggaggtatt tcctcgattg ctgtgcaaaa
     6061 gttccacgtt tgggcccccc ggggtctccg tggggaccga gtgggtctct gctcagttca
     6121 ccatcaccag tttgcccttg acgccgcgcg agcccatgtg ggcgtacgcc gccttgagtt
     6181 cggccatggg catggtgcgg tcgatgaccg gcttgatctt gccttgcgca taccactgcg
     6241 ccagttcggc catggcggcc gcactggcct tcggctcgtt tttggcaaag ccgccccaga
     6301 acacgccgac gatggacgcc cccttgagca gggtcaggtt cagtggcaat gaggggatgg
     6361 ggccggacgc aaaacccacc accaggtagc ggccgcgcca ggcaatggag cgaaaggccg
     6421 gctctgcaaa atcaccgccc acggggtcgt aaatcacgtc ggggcccttg ccgtcggtca
     6481 gcgtcttgat ggcctcgcgc aggttctccg tgctgtagtt gatggtggca tcggcgccta
     6541 tggatttgca cagcgcgcat ttttcgtcgg tggaggcagc cgcaatcact ttggcgccca
     6601 tggccttggc gatctggatg gccgaggtgc cgacgccgcc ggccgcgccc agcaccagca
     6661 cggtttcacc ggccttgagc tgtgcgcggt ccaccagcgc gtggtgcgag gtggcgtaaa
     6721 tcatgatgaa ggctgcggca tcaacatacg gaaagccgtc cggcagcggc atgcacaggg
     6781 ctgccggcgc gatggtatgg gtgccgaaac cgccggtgcc ggagagacag gccacgttct
     6841 ggccgacctt caggtgtttg acaccttcac ccacggcctc gaccacaccg gcgtattcgg
     6901 agccgggcac aaagggcagc ggcggcttga tctggtactt gttctgcacg atcagcaggt
     6961 ccgggaagtt caggctggcc gccttgatgg cgatacgcac ctcgccggct ttgggctcgg
     7021 gggtgggcag ttctttccag gtcagggcgt caacgccggt ggggtcttca caaagccagg
     7081 catgcatggg gagtctccgt tggggatcaa gtggacccat cataggcagg ttgcgggacc
     7141 aaatagacgc ggcatggtcc cggtttgtca cccttgagac cttaaaatcg gctcatgaaa
     7201 atcctgatct gtaacgacga cggctaccag gcctccggca tcatcgccct gtacgaggcc
     7261 ctgaaggaca ttgccgacgt ggaggtcgtt gcacccgagc agaacaacag cgccaaatcg
     7321 aacgcgctga ccctgcattc gccgatgtac gtgcagacgg cagccaacgg gtttcgctac
     7381 atcaacggca cgccggccga ctgtgtgcat attgcgctga ccgggctgct cggttaccgg
     7441 cccgacctgg tggtctcggg catcaacaac ggtgccaaca tgggcgacga cacgatttat
     7501 tcgggcactg tcggggccgc catggaaggt tatctttttg gcattccctc gattgcgttt
     7561 tcacagaccg aaaagggctg ggcccatatc gacgtggccg ggcggcgcgc gcgcgatctg
     7621 gtgcagcagc tgatgccttc gctcgagaag atcagcgaag gcgcgcagcc gacgctggcg
     7681 ccctggctgc tcaatgtgaa cattcccaac ctgccggacg cgcagctcca gggctacaag
     7741 gtctgccgcc tgggccggcg ccacgcggcc gagcgcgtga tcacccagac cagtccgcgc
     7801 ggcgaaacca tgtactggat aggcggtgcc ggcgcggcca aggaagaggg cgagggcacg
     7861 gacttttttg ccacccggca cggctttgtt tcgatcacgc cgctgcaggt cgacctgacg
     7921 gaccatgagc ggctgcccta ctgggcgcag acggccgccc agctgagcca ggccgtccgc
     7981 agcggtgcgg tggctgaacc cgcccaggcg gcggcatgaa gtccccgctg aagccttcct
     8041 tcaagccgac cgctgcggcg gccaatgcgg ccggcgttac gccggcccgg cggccgggct
     8101 ttccggtgcg tctcgagccc gatgccggca cgccatctgc taccaaaaag atagctggcc
     8161 gcgcccatcc ggaaagggat tcaggcattt ttgactccaa aaaaccggcc aagaccgtgc
     8221 tgacgagcgg tgtcggtctg gactccaggg cggtgcgtac gcgcatggtc agcaagttgg
     8281 cggcacaggg catctcggac gcccaggtgc tcgccgccat gggccatgtc gagcgccacc
     8341 gctttgtcga cacggcgctg gtcaaccagg cttatgaaga caccagcctg ccgatcgggc
     8401 tgggccagac gatttccaag cccaatgtgg tggcccgcat gctcgagctg ctgcgccagg
     8461 gcgctggcgg caccgacggc aagctgggcc gcgtgctgga aatcggcacc ggctgcggct
     8521 accaggcggc attgctggga cagctcgccg ccgaggtcta cagcatcgag cgcctgcgcg
     8581 ggctgcacga caaggcgcgc gagaatttgc gggcctttcg cctggccaat gtgcacctgc
     8641 tgtttggtga cggcatggcc ggttatgcca agggcgcgcc ttacgcggcc atcattgcgg
     8701 cggctggcgg cgaggcgatt cccccggcct gggtcgaaca gctcgcggtc ggcgggcgcc
     8761 tggtggcgcc catgcatgtg gccggcggtg cccaggcgct ggtggtgctt gacaggacgg
     8821 cgaacggtgt tacacagact gttctggagg ccgtgcactt tgtaccttta aaatcgggaa
     8881 ccagctgagc aggcatgaac ccaggtacgt tcatggtgtc tccccgcgtc atgaacgcag
     8941 aattgacaag cagctttgca aaaagggtga atcaagcaat gaatgaacaa gccggatcgg
     9001 ccatgacagc gtctttttcc accgctcctg aaagccaccc cggcggacag cgtgctggcc
     9061 gtctgctgac gacctgtttt ctggccgccg cagtcctgct gctgtccgcc tgcggaacgc
     9121 gcagcctgag ccgcgcgccg gtcgaggacc gcggcgccgg catggggcgt gcgccggtgg
     9181 gcgcggttga tcccgcgggc gtcaagcagc tgcctggttt tgagaatgcc ggcaagcctg
     9241 gctactacac ggtcaagccg ggcgacaccc tgatccgcat cgggctggac accggccaga
     9301 actggcgcga catcgggcgc tggagcaatc ttgaaaatcc caacctgatc gaagtcggcc
     9361 aggtgttgcg ggtgacgccg ccggtcgaga gttcggtggc cgtgtcccgg cccgtgacgt
     9421 ccggctctgt cacgccaact gccccgacat ctgccagcag cgctccggcg accgcgagcg
     9481 ccagcgccgc caaaccggcg gcatcggcac ctgcctccgc gtcgggcggc gaggacgatg
     9541 tggcctggat ctggccggcg cagggtcctg tgctggccgg ctttgacgag gccaagaaca
     9601 agggcatgga catcgcgggc aaaaccggcg acccggtgat ggcgtctgcc gacggccgcg
     9661 tggtctacgc cggcgccggc ctgcggggtt acggcaacct gatcatcctc aagcacaaca
     9721 acaccttcct gaccgcctac gcgcacaacc agaccctgct ggtcaaggaa gaccagtcgg
     9781 tgcgcaaggg ccagaagatt gccgaaatgg gcgccagcga caccgaccgc gtgaaactgc
     9841 attttgaaat ccgcaagcag ggcaagccgg tggatccggc caagtttctg cccgccaaat
     9901 aagcggacca ccgccgcgaa ccaagaagcc ctgcatgcag ggcttttttc atgacccggt
     9961 ggcagcccgt tgatgcatca aattggccgc cagcccttgt tcggcgggcg caagctgcta
    10021 tcagcttggt agcgaatcct ggccggtggg gatctcgtca taaccgcggc cggtgaactg
    10081 caacaggtca atgccgtggc caaacgggtc ggccagcccg gcgatgcggc cccagacgcg
    10141 gtctgtcacc ggctgctcga gtacgacgcc gtgctgcagc agccggctca cggcggcgtc
    10201 gatgtcgtcc accacaaagt ccaggtgcac cggtgtccag tggcgctggt agctgcgcgt
    10261 ggcggcgctg ccgggcaacg ggcggctgcc ttcggtttgc agcagcaggt cgatcgggct
    10321 gcccgcgccc agcaactcga caaagccgct ctggaagcgc cggccgcagt gcaggccgag
    10381 tccttcggtg tagaacgcga cggcgcgttc catgtcgttc acgtcgatgc agatgcgtat
    10441 ctccatgagg tcctccttgt ccgtctgttg gctcagccga aaatggtaac ttggtactgg
    10501 aacccgaaaa tatcgaaaca tgaaagcgcg ccttcgcacc catggcggcg ttgcaatcct
    10561 tgcgaaggcg tacagccttc gctgcggctt gcgccttgcc ctgggcacga agtcatcact
    10621 ttcattatgt ttcgctactt ccgggctgca gtacaggcct gagacaatgg gggatgtcca
    10681 gaagaaaaca ccagcagccg cagcaccagc cggcccccgc gccaaccgac tacccgcccg
    10741 atgtgctcac cgtcgagtcg ctcgacatcg aagcccaggg cattgcgcac cgggacgacg
    10801 gcaaggtggt gttcatcgaa ggcgccttgc ccttcgagac cgtcagcgcg agcgtttacc
    10861 gcaagaaacc gagcttcgag aaagccacgc tgatggcggt gctgcacgag tcttcgcagc
    10921 gcgtgcgtcc gcagtgcccg cacttcggcc tgcacaccgg cgcctgcggc ggctgcaaga
    10981 tgcagcacct gcaggtgggc gcgcaggtgg ccgtcaagca gcgtgtgctg gaagacaacc
    11041 tctggcacat cggcaagctc aaggccgaca tggtgctcag gccgattgaa ggcccggcct
    11101 ggaattaccg ctaccgggcc cgcctgtcgg tgcgttttgt gcgcaagaag ggagaggtgc
    11161 tggtgggctt tcatgagcgc aagagtggtt atgtcgccga catgcgcgag tgccacgtgt
    11221 tgccccggca tgtcagcgac ctgctgctgc cgctgcgcgc gctgatcgcc ggcatggacg
    11281 ccaaggaaac cctgccgcag atcgagctgg cctgtggcga cacggtgacg gccatggtgc
    11341 tgcgccacat ggagccgctg tccggcggcg acctggacag gctgcgcgct tttgcgctgg
    11401 cccatcccgg cctgcagtgg tggttgcagc ccggcgggct ggagacggtc aggctgctcg
    11461 acgaggtgag cgatccttcg cagcagctgg cctacggcct gcccgactac ggcatcacca
    11521 tgcccttcaa gccgaccgac ttcacccagg tcaacccgca gatcaaccag gtgctggtgt
    11581 cgcgcgcgct gcgcctgctg ggcgtgcagg cgcacgagcg cgtgatcgac tggttttgcg
    11641 ggctcggcaa tttcaccttg ccgctggcca cccaggcgcg cgaggtgctg ggcatcgagg
    11701 gcagcgaggt gctggtcgcc cgctcgcgcg aaaactatgc atcaaacaag gcctcagccc
    11761 agaaggggcg ggcgctggca gctaccaaat ttgtagcgcg caacctgttc gagatcagcc
    11821 ccggcatgct ggtcaaggac ggcgccgccg acaagtggct ggtcgatcca cctcgcgaag
    11881 gtgcgttcga gctgttcaag gcgctggccg cgctgcacca gcagatcgtc acgggcgtgc
    11941 cttgtgatga cgccggcggg caacaaaacc tgcagatcgg cgactggatg ccaccgcagc
    12001 gcatcgtgta tgtcagctgc aatccggcga cgctggcgcg tgatgcgggc gtgctggtga
    12061 atgacgcggg ttaccgctgc acggcggccg gtgtggtcaa catgtttccg cacaccgcgc
    12121 atgtggaatc gatggccgtg ttcgagcggc tcccggcaag cgccggttag cagggggcgt
    12181 aaaaaggctg tccgccatga cagcggacag cccgtcccaa gccgttgacc ggcgactcag
    12241 tgcttggcaa ccgtcttgcc tgtggtgtgt gccttgtaga tccccgcgtc tgccgcaccc
    12301 gtgacttcca gcatgccgac caggcccttg ccggcccgcg acagcgcgtg gtccaccagc
    12361 aggtagctgc cggggtagtc caccttgaac tcgacgatgg tggcgccgcc gggtgccacc
    12421 agtgtggtct gcacatcacg tttgggcgag ctgatcgagc cttcgctata gaccttgtcg
    12481 aagatttcgc cgatcacgtg gaaggacgag atcttgttcg ggc
//
LOCUS       DAIDFG010000058        12371 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_58_length_12371_cov_19.186749, whole genome shotgun sequence.
ACCESSION   DAIDFG010000058 DAIDFG010000000
VERSION     DAIDFG010000058.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 12371)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12371)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12371
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58_length_12371_cov_19.186749"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2455 a         3850 c         3833 g         2233 t
ORIGIN      
        1 ccagccggga acccacgcgg tcgtccacga agtggacccg aaaaccgcca acgctgacat
       61 cgtccatggc atcgatcgcc ttgtggagtt tggcccgcgt caggtccttg ccggcccgcc
      121 gcagcgcttc ggtgtacacg cgggcggcga tgtagccttc gagcatgtac acattgggct
      181 tgcccagctt ggcggcaagc gcatcggcct gcatgtcacg caccagggcg accttgctgc
      241 tgtcgctctt gggaaccacc cgcgccacca ccacaccagc ccccgtcttg cccagctgct
      301 cggccagcag gctttcgccg gtgttggaga aaccgtaaat cggcccgcga aagcccttgg
      361 tgcgcaggtc acgcacggcg ttggcggcac ccgccgcgtc gctgatcacc agcaccgact
      421 ccgggcgcgc ggccagcgcc ttgtccacgc tgccggccag gctgccgctg gcaaaggggg
      481 ccgtggccac caggttggcg cccaggctcg tcatggtgcg ctgggccgag tcgagggcgg
      541 ccatggattc gccatcaccc gcatgcagga tggccagctt gcgcgcgccc agggtggccg
      601 catgtttggt aatggccgcg gcctcttccg aataaccggg acgcagggag tagacattgc
      661 catacagcgc cccgcgaaac tcgtcggccg ccgccatggg cgcaaacagc aacacgtcgc
      721 cgtccttgat cagggggtag gccgcggtca cctgcggcga gccgtaaaaa ccgaacagcg
      781 aaagcacacc ctggtccagc agctttttcg tgttggccgc cgtgttggcg gggttgccga
      841 ggtcgtccag cgtgacgagc tcgatcttgc ggccgttgat gccgccggcc gcgttcacgc
      901 tgtcgaaata caacttcgcg ccctggtgaa aaggctgggc cagcgaggag cctgggccgg
      961 tcagcgccag cgactggccc agcacaatgc cggtcttgct cacgccgtcc tggctgtgcg
     1021 ccactgcacc ggcgagcgcc aggacagcta cgataagggt ctgtcccaga gtgtgtaatt
     1081 ttttcatatt gattgccctg gctgaaacgc aagcagggtc ggctcctagc aagaaaatgt
     1141 agttccattt atgtctgcct ccatcagcat ctcccctagt gccgctcccg cgaaaaacac
     1201 cgtcaaggtg cggggcgcct gcccgcatga ctgcccggac acctgcgccc tgctcaccac
     1261 ggtggaggat ggcgtggcga tcaaagtcca gggcaacccc gaccaccccc agaccggcgg
     1321 cgtgctctgc accaaggtct cgcgttacac cgagcgcagc taccacccgg aacgcatcct
     1381 gcagccgctc aagcgggtgg ggcccaaggg ttcgggacag ttcgagcctg tcagctggga
     1441 caccgcgctc ggcgagattg ccgcgcggct acaaggcatt gctcagcgcg acccgcaggc
     1501 catcgcaccc tacagctacg ccggcaccat ggggctggtg cagagtgaaa gcatggcggc
     1561 gcgctttttc aacaagctgg gcgcctcgct gctggaccgc accatctgtt cggccgccgg
     1621 cggcgagggc ctgacgcaaa cgctgggccg caaggccggc atgaaggttc agttttttgc
     1681 cgaagccaaa ctgatcctca tctggggcag caactcgatc gccagcaacc tgcacttctg
     1741 gcgttacgtg caggaggcca agcgccaggg tgcaaagatc atctgcatcg atcctcgcaa
     1801 gaccgaaact gctgaaaagt gccacgaaca catcgccctg cagcccggca ccgacgccgc
     1861 gctggcgctg ggcatcatgc atgagctgat cgtccacaac tggctggacc acgcctacct
     1921 tgagagccat accatcggct ggcccttgct gagagagcgt gcactgctat ggaatccgga
     1981 gcgcgtggcg caggtctgcg gtgtacccgt ggagcaggtg cgctctctgg cgcgcgacta
     2041 cggcgagtgc tttgtgaacg ggcagccggc ggcaatccgc ctgaactacg gcatgcagcg
     2101 cgtgcgcggc ggcggcaacg ccacacgcgc ggtggcctgc ctgcccgcgc tgaccggggc
     2161 ctggcggcac cgcggcggtg gtttgctgct gtcgagttcg ggcatgttcc cggtcaacca
     2221 ggccgcgctg cagcggcccg agctgcgcgc cggccggcag ccgcgcaccc tcaacatgat
     2281 caccatcggc gacgacctgc tcagaagcag ttcggccgcc ttcggcccga agatcgaggc
     2341 gctgatcgtc tacaacagca acccggtggc ggtggcgccg gagtctggca aggtggtgca
     2401 gggttttgca cgcgaggacc tgttcaccgt ggtgctggag catttccaga ccgacagcgc
     2461 cgactacgcc gatttcatcc tgccggccac cacccagctc gaacactggg acgtgcacag
     2521 cgcctatggc cacaccgatg tgctgctgaa ccggccggca attgcccccg tgggccaggc
     2581 ccggcccaac accgagatct tccgcggcct ggcggcacgc atgggcttca ccgagccctg
     2641 ctttgcggat gacgacgaaa ccctgtgccg cgcggccttc agccacgcgg acggcgacaa
     2701 aatcgatttc aacgaactgc tggacaaggg ttttgccacg ctgcccggcc ccgacgcgcc
     2761 gtttgccgag ggccgctttt tgaccccgtc gggcaaatgc gaatttttca gcgaacggct
     2821 cgccgcacag ggactggacg gcctgcccga ccatgtgccc aatcacgagc tggcaggcag
     2881 ctcggccagc tacccgctgg cgatgatctc gccgccggcg cgcaacttcc tgaactccac
     2941 cttcgtcaac gtcaaaagcc tgcgcgacat cgagggcgaa ccgctgctgg aaatcaacgc
     3001 gcaggacgcc gccgcgcgcg gtatcagcac cggcagcgtg gtgaaggtgt tcaaccagcg
     3061 cggcacctac cgctgcaagg ccagggtgtc tgacagggcg cgcgccggcg tggtccacgg
     3121 cctgggcatc tggtggcgca agttcggcct ggatggcacc aatgtcaacg aactgaccag
     3181 ccagcacctg accgacctgg ggcgcggccc ggtgttttac gactgcctgg tcgaggtgca
     3241 acttgacgcc gcgccagcac tctgatctgg atgccctcct tccggtgctc ccccaaaaca
     3301 acggccctgc ttgcgggcgt gctgctgggc ctgtccggtt gtgccagcct cggttattac
     3361 tggcagtcgg tcagcggcca tctgcagctg atgaacgccg cccggccgat tgatgactgg
     3421 ctgcaggaca gcgacacccc cgacaaactc aaggccaggc tggccctgac ccagcagatc
     3481 cgccgctttg cggtaagcga actgaatctg cccgacaacg ccagctacca acgctacgcc
     3541 gacctgcggc gcaaggccgt ggtgtggaat gtgtcggccg cgccggagtt ttcgctgacc
     3601 ctgcatacct ggtgttttcc ggtcaccggc tgtgtaggtt acaagggtta ttttgacgag
     3661 gcccaggcac gcaccgaagc cgacaagctg agcgcgcaag ggctggaagt cagcgtttac
     3721 ggcgtgccgg cctactcgac cctgggctgg atgaactggg ccggcggcga cccgctgctg
     3781 agcaccttca tcggctaccc cgagggcgaa ctggcgcggc tgatttttca tgagctggcg
     3841 catcaggtgg tgtatgccgc cgacgacatg gacttcaacg aatcctttgc caccgccgtg
     3901 gaacgcctgg gcagcgcccg ctggctggcc acccacggca gcgccgccgt ccgcaaagag
     3961 tacgcgctgt acagcgaacg ccgccgccag ttccgcgcac tgacgctggg gctgcgccgt
     4021 gagctcagcg agatttatga gccaaaaacg gccccagccc ccgaccgccg tgcgcaagca
     4081 gctatgaaaa acatagcaat tcagaccttc cacacgcgtt acgccgccct caaggccggc
     4141 tgggacggct acgccggtta cgacccctgg gtggcccgca ccaacaacgc ctcgctgggc
     4201 gcgctggccg cctatgatga actggtgccg ggcttcgagg cgctgtttgc gcgcgaaggc
     4261 agcaactggc cgcggtttta tgatgcggtc agacgcctgg ccgccctgcc caaacaggag
     4321 cggcacgacg ccctccgccc ttccatcacc acaaaaaccg aggaaacacc ccatggctga
     4381 catccatatc gaacgcgagc acagcctggg cctggcccag gcccgcaaga tcgccttcca
     4441 gtgggccgaa caggtcgagg aaaagttcga catgagctgc acctacgagg aaggcgacag
     4501 ctgtgacgaa gtcagcttca cccgctccgg cgtcagcggc acgctcaagg tgaccaagga
     4561 ccagttcgag ctcgacgcca aactgggctt tctgctcggc gccttcaagg acaggatcga
     4621 gggcgagatc gtgaaaaacc tcgacacgct gctggcccag aagaagcccg ccgccaaaaa
     4681 gaaacctgcg ccgaaatgac ggcaacggcc catccgggcc cgctggcggg cctgaaggtg
     4741 gtggaactgg gccagctcat cgccggaccc ttcgccgcca agacgctggc cgacttcggc
     4801 gccgacgtca tcaagatcga agccccccgc gcaggcgacc cgctgcgcaa atggcggctg
     4861 ctcaaagacg gcacctcggt ctggtggcag gtgcagtcgc gcaacaaacg ttcggtggca
     4921 ctcgatttgc gacaggcgga agcgcaggag atcgtccgca aactcgcgct ggacgccgac
     4981 gtgctgatcg agaacttccg gcccggcgcc atggaaggct ggggcctggg ccctgacgag
     5041 ctgctggccc tgaacccccg gctcatcatg ctgcgcatca gcggctacgg ccagaccggg
     5101 ccctaccgcg accggcccgg tttcggtgtg gtggccgaag ccatgagcgg cctgcgccac
     5161 ctgaccggcg aaccgggccg cgtgccggtg cgcgtcggcg tcagcatagg cgacacactg
     5221 gccgcgctac acggcgtgat cggcatcctg ctggcgctgc aacaccgcca tgtctcaggc
     5281 aaaggccagg tcgtggatgt ggcactgtat gaggctgtct tcaactgcat ggaaagcctg
     5341 ctgcccgaat acagcgcctt cggcgccgtg cgcggcccgg ccggcagcgc cctgcccggc
     5401 atcgcgccca gcaacgccta tgcctgcaag gacggcggct acgcgctcat cgccggcaac
     5461 ggcgacagca tcttcaaacg cctgatgacg gtgatgaatc ggcaggacct gggcaacgac
     5521 cccgcgctgt ccgacaacgc cggccgcgtg gcgcgcgtgc aggagatcga cgccgcgatc
     5581 ggccaatgga cggccggcct gacggtggaa gaagtgctga cggcgctgga aagtgccgag
     5641 gtgcccgcgg ggaaaatcta caccgtcgcc gacatcgccg ccgacccgca ctacgccgcg
     5701 cgcggcatgc tgcaaaaagt gcagatggac gatggctcgg aaatcgccgt gcccggcttc
     5761 gtgcccaagc tctcaaccac gcccggcagc cagcggcgca atgcaccggc cctgggtcag
     5821 gataccgatg cggtgctgcg tgaggtgggg ctgacggagg cacagatcgg tgcgctgagg
     5881 cagcgtgggg ttgtggggtg aacggcaggg caatcgcatc aaaataaagg acagcaaaag
     5941 gtcaaacaag ggaggtgatt gatgcgagag gcaaactacg ggacttcaaa acagcctgta
     6001 gaaactctcc tgcatgacag acaaagcgca cctgagcctg atggactgct ttggccaagt
     6061 gccagatccc cgcattgagc gcagccagcg gcatgaactg ctcgatatat tggccgttgc
     6121 cctgtgcgca gtcataggcg gggcagatca ctggacggaa gtggtcgaat tcggtgaatc
     6181 caaactggcg tggttctcca ccttcttgaa gctgcccaat ggcattgctt cgcatgacac
     6241 ctttgctcga gtgtttcgcc tgatcaacac cgatgcccta gagagcgcct gccgccaatg
     6301 gcttgccagc ctggtcgggc gcgtggctgg ggtggttgcc attgatggca aaagcgtgcg
     6361 cggctcgcgt gatggcaaaa agcacccact gcatatcgtc agcgcctggg ccagccagca
     6421 cagcgtgctg ctcgggcagg tcaggacggc ggagaagtcc aatgaaatca cagccatccc
     6481 ggagttactc aagctcttga gcattgaagg ctgcatagtc accatagatg ccatggggtg
     6541 ccaaaagagt attgccaagg acatcatggc cagcggagcc gactatgtgc tgagccttaa
     6601 aaccaatcac gcccacctgt gccttggcgt ggctgcctgg tttgacaagg gcctggttgg
     6661 cggctttggt aaacatgcct gcagtcatta cctggagccc gcccaaagcg gtcatgggcg
     6721 cattgagaag cgagagcact gggtcacgac ggtgccccag caccttaggc gggccaccaa
     6781 ggcttgggat ggacttcaaa ccatagcgat ggtgcgaagg cagcgaaaaa taggcgacaa
     6841 ggccagcagt gaggacagct actacatcag cagcttgccc atgagcgcag gggcacaagt
     6901 gttagcccat gcggtgcgaa gtcactgggc ggtagaaaac gagttgcact ggtcgctgga
     6961 cgtgggcttt cgggaagatg cctgtcaggt gcgcaaagac aatgcgcctg ccaaccttgc
     7021 ctgcatcagg cgcatggcgc tgacccagct caagcaggag acaactaaaa agctgggcat
     7081 tcagggcaag cgccgacgcg ctggatggga taccgcatat atgcagactg tgctcaaaat
     7141 ggtaccggtt tagatgcgat tgccctgggg tgaacggccg gtcgctacgt tattgatagc
     7201 tgatggcgca tatggcgtat gggctggagc tcgatttggc tacaagaccc tgccgctcct
     7261 agaatcatgc ggtgtttggt gttcggttta actacatgac agggagcatc catgcataaa
     7321 aattcctggt gcgacaagga cttggcagca aatccgttgg ccgagttatg cggcaggccg
     7381 tgcggtttac attacgttgg acatcacaga tgatggtgac tgaggctctc ggcattgccc
     7441 ttgcagtagt gggtctagca tttgcctttg aaacgccccg ccactggttt cttcggacaa
     7501 ctcggctggt caagcctcgt ccgagtaaaa ggaacgcggc cgaacctgca atcctcactc
     7561 caaagccggc gccagctgtt gccccaccat tcgcggtatc gtccatcaca gttatggaaa
     7621 tcgttgacac gataaatgca gcgccaccat ttcagcgcaa gcagctggcc cagaactact
     7681 ccgggattca cattagctgg gagggatacc tcaggcgagt cgatgaggac cccgtcaatg
     7741 tctcccgcgt ccgagtcaat ctggctgttg atgaatcgga gatccttgga tactcaatct
     7801 ggtttagcgt atcacccgac aggcttcccg agattaaaac catgccgcga aactccaggc
     7861 tccgagttgt cggaaagatt ttgtcggcga gtggctcggg tttgtgtgtc gatgtggcca
     7921 ccgaagaaat tgaggtcgtt gatcgtgccg gttagttgcc tcgtcctcgg agcggtgaaa
     7981 gtgacgccta gctccgacgt tggaaaatca ccatgaacgt atttctccga actcttttgc
     8041 ctcttacgtt tgctgcgctc atcgtcggtt gcgattcgaa cggcgacacc ttcactcttt
     8101 atcgcaacag cgtcacggac gaaaacatgc gaatccatgt tgcttcgttt aacgcttcgg
     8161 atggtgagtc ttataaccgg ggcaactgcg agcaagctca attgctcttt caagctcagc
     8221 ccggagtaaa aacgaaattc tggtgtgaga agggcgtgtt cagaaaatga gccaattgcc
     8281 taacgcagcg cttatgctcg ttcccttcag tcgctggacg ctgcgcgaaa tagtcgcgca
     8341 gcgccagtga cttctacgtc aggcccaacg tcagccaccc cacccatccc acatgcgctt
     8401 gcccattctt ccggaacgac cccacgccgg ccccatgaaa cctgtggctt ctgcgaagac
     8461 aacgcaggcc atcctcccca gcggccaact tgagctcacg atagcgcacg acaccgtccg
     8521 aggcgtcaca ccaaccatgc tttgctggtg gttcgagacg ctcggtcaga cgatgacctg
     8581 ggaaggacgg acctatcccc ggtacctgct gtggcatccc cgcgaccaca tccagtggga
     8641 gctggccgcc aggtcgccca ccggtggaag cggacagggc gcacactttc gcatcgtcga
     8701 agccttcgcg gccaaccccg cgtactacgt ggactcggcc gagtgggtcg agaagctcga
     8761 tgaagagggg atcgcgctcg tgcggcgaat cgccggcgtg gaggtgttcc ggctggagca
     8821 ccgtttcggg caggcagcag gcggtgcgag ctaccggtcc cgcatgacgg taggcgtggc
     8881 gtccggcctg tcccggcgcc ccttcaactt cttcgtccgg ccaaaggtct tttcagacgt
     8941 aatgggcaca gcctggctca cgcacaacgt cgaggaggtc agcatgctcg agaatattct
     9001 tcccgcgttg tatgcggcgc gatgaggcct ggaacgatgc tgaacatgcc tccaagtttt
     9061 gccgtgtaaa ttacgtcaaa agacgcaatt catggaacac tatctcgaac tggcagccgt
     9121 ggcactgtcg atcatcgcgt ttgtcccggc aacgttcttt ttcctgcgcg ccagttacca
     9181 attcgtcctg atgcttggac actaccgcag cggaaaacat cggctcgggg caaacctctt
     9241 gccattcctg gtgccgttca tgccccaagt ctttaccgag caaggcaatc tgcatcggca
     9301 ggcgttcttg tccaatttgg gatggtcact gtgttgcatg gcgttcatcg ccattatgta
     9361 cacggtcctt ggtgtcgcta atgcgtagct cagcgtgtct caagtggtcg tttgccgtga
     9421 acacgcagca ggaaaactca tgcagaaacc gccaggtgca gtaagggctt tgacatcaga
     9481 tctgaaaact gagctgcacc gaagcgccca agttttacct cagtatgtgc gtcatacatt
     9541 cagtgaggcg ttatgaaact atctacaagc ggccgtagaa atattctcgt cgctctatgt
     9601 tttctgttgc tgatggggtt gatggcgtta tccaccttta ttccttcaac cgaacagcgg
     9661 tgcaaatcga cctgccgttc caaaggattg gacggtcgca tggtctatct gtatccaaaa
     9721 gaaatgacag ccggtatgag aggtagagga ccatcggaat gtcagtgttc tcgcccctga
     9781 cattcattgc agccagcggg attgggcgtt gaatgcaact gctagccaga acagtcggca
     9841 cggattaagg ccggccgacc accgcttgca gcaactcagc tcaacgactc aatcaaatcc
     9901 acatactgct gcatcgcatc ttcctgcgag gtgcccttca ggttgttcca ggcgtcccac
     9961 ttggcgcgcg ccaccatgtc gccaaagccg ggcttcttgt cggcgttgtc accgctgctg
    10021 ccctgcttgt agagcgcgta gatcttcagc agcgtggcat tgtcaggccg ctcgctgagg
    10081 ttcttggaat cggccatggc cttgtcgaaa gaggctttga ggtctgacat gaagaggctc
    10141 cgtgagggtt ggaagtggaa atgaattcgg gtaactgccc ggtatcttag ccactgcctt
    10201 gactgcaaaa tagggaattc accccatccg gggtgcttgg tacacggagt tttcatggtc
    10261 acacgcgttt cggcccaggc cgcaggcaaa aaggttgcga agaacgtcca gaaaaacgtc
    10321 aagcgcgcgg tctcgggcac cttgggcctg tcgggtgaac gcatgagcaa ggtcgacacg
    10381 gcctggctgc gcatggacac gccgtccaac ctgatgatga ttgtcggcgt ctggcaactt
    10441 gcgccgggcg tcagccacgc ggcggtctgt gaacgcatgg agtccagcct gctcaaatac
    10501 gcgcgctttc gccagcgggt gatggaagac gccgccggcg cgacctgggt cgaggaccgc
    10561 tacttcgacc tgcagaacca cgtgattgcc gacaagctgc cgaaggtggc gaagggcaag
    10621 gaacaggaag cgctgcagga ccgtgtggcc gaacttgcca tgcagccgct ggaccgcaag
    10681 cgtccgcttt ggcaaatcca cctggtcgag gactacaccg gtcccgacgg cgtcaagggc
    10741 agcgccatga ttgtgcgcat ccaccactgc attgccgacg gcattgcact gatttcggtg
    10801 accatgtcgc tggtggatgg tggcgcggcg ccgcccgagc gacgcaagaa ggaagccggt
    10861 ggcggcgccg aagactggat cgccgagacc ctgctcaaac ccttcaccga cctgacggtc
    10921 aaggcgctgg gcgcggccgg cgaaggcgcc gccaggtcgc tgaacctgct gagtgacccg
    10981 caaaaaggca tggctggctc gctcgacatg gccaaggtga tgctgcaggt gctcaaggac
    11041 agcgcggcgc tggcgctgat gcccgacgat tcgaaaaccc ggctcaaggg caagccgggc
    11101 accaccaaga aagtggcctg gtgccagccg ataccgctgg acgaagtcag ggccatcggc
    11161 aaggcgctga actgctcgat caacgacgtg ttgctcagtt gcgtggccgg tgcgattggc
    11221 gaatacctca agtcgcacgg cgacgacgtg aaaggccagg agatacgcgc catggtgccg
    11281 gtgaacctgc gccccatcga gcacgcctac aaactcggca accgctttgg cctggtgccg
    11341 ctggtgctgc ccatcggcgt ggacaacccg gtggagcggg tctacgaggt gcggcggcgc
    11401 atggcagccc tcaagggcag ctaccagccg ctgctggcct tcagcctgct ggcgattgcc
    11461 ggcctgctga tcaagccggc gcaggacgcg atgctcaacc tgttctcgaa aaaaaccacg
    11521 gcggtgatga ccaacgtgcc gggaccgcgc gaaaaactca agttctgcgg cgccacgctg
    11581 gagcaaagca tgttctgggt gccgcagtcg ggcgatgtcg ggctgggcgt gtccatcctg
    11641 agttatggcg ggggcgtgca gttcggtgtg attaccgaca gcaccttgtg cccggaaccg
    11701 cagcgcatca tcgacgagtt tgtgcccgag ttcgccaagc tgttgatggt gacaatgatg
    11761 cttccctggg gcgaataacg ctggacctgt aagcgtttta tgccgtttgc cctttggatt
    11821 cgggcgcgag cagctattta attgatagca aatcgaaagc atcacgctgc gtccgtggtc
    11881 tgccatgaat agcaggcgta attccccact ccctcccccg ctgggcgatg gcggagagct
    11941 tcatttggcc gcgtgtgctg cgctcgtgtg catgaatgac cgctgcgtgt tccgacgatg
    12001 acgcgcaaag cgcgtcatcg tctccccgaa tccgctcccg gttttatccc ccacccttct
    12061 ggctgcgccg aggagcggag ggccagacgg atcagggcga gcgattgtct gagccgaagg
    12121 cgagttcgag ctcgaccccg gctggaccga gcaccgcagg ctgcccggag cgaagcgcag
    12181 ggacgcagac agcagggtcg ccttctcttt gcttactttc tcttggcgac acaagagaaa
    12241 gtgagtcggc tgccgggccg agacccggct tgctggccac cccgagaagt tggaatcgaa
    12301 gacggcttcg acaggctcag cccgaacggg agaaggaacg ccggcctcag cccttatgga
    12361 acaagcgcaa t
//
LOCUS       DAIDFG010000059        12351 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_59_length_12351_cov_18.541233, whole genome shotgun sequence.
ACCESSION   DAIDFG010000059 DAIDFG010000000
VERSION     DAIDFG010000059.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 12351)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12351)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12351
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59_length_12351_cov_18.541233"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2176 a         3835 c         4321 g         2019 t
ORIGIN      
        1 ggtgccactg tggcgcccat cgcctgcagg atggaaattt tggaaaacgc caacaggcca
       61 aaacctgcga cggtggtgag attggccagc gccatcgaag ccagggtgcg gtgtgagatg
      121 ggcgtgagcg ggtcgggccg gtcgaaaaac agggcgtagt tcgaacccac cgccaccacc
      181 agcagcaagc ccaccagatg caggatgatc agcggctggc cggccagggc caggcctgcc
      241 gtgacggtga tgaccgaggc gaccaggggc gcgatgacgc gcaggacacg catgggcgag
      301 cggaacacca ccagcagcag cacgacgatg gagagcagcc cgcccagcga cagcagcgtg
      361 gcttcgcgca ggtagccggc gtacaggcgg tcggattcgg ctttcatgtc cacaaacagc
      421 acatccggcg agttgctgcc cagggctgca cgaatccggt cggcctggat gcccgcgcct
      481 tccggcgccc gaagcggcag cagcgcggtc cagccgccac gcgatgtctg cagcagcagc
      541 gcatcgaccg ccatggctag ggaggtctgt tcgaggtcgg acgcctgcag caagggccgg
      601 ctgcggctgg tttccacgtc ggccagaaag ggcgtgaaca cttgggcgcg caccggcagg
      661 ccctgcaccg cctgcgccag gcgcggctgc aacacctccg ccggcggcag gctggcctgg
      721 cgcgcacgct gcgtcgccat gccgggaagg tagcggctag ggctttcaaa cccggcgagt
      781 tcgccctggt ccacctgcgt ctgcagcaat gcggacacct gttcggcgga caccagcacc
      841 gattcccggc tgctgccgga gatgaccacg acgtaacgca catcaggggc gcccatgtcg
      901 gcgcgcaggc tttcgtcgag tgcgatgtcg gcttgcgaca ccgggctcag cgaggagatt
      961 ttttcgttga gcagcgtgtc gcggtgggtc gcgaggacgg cgcaggcggc cagcagcagc
     1021 aaggccgccg gccagcgcag cgctgcggcg cgctgcgtca gccggctgag cacgcggccg
     1081 atggccgaga tgtcgtggat gcggaactgc gccggcaaca ggtgtggcag gacaaaacgc
     1141 gtcatgaccg cggcggccac cagtccggca atggcgtaca ggcccagctg cgccaagccc
     1201 ggaaagcccg acagcagcag cgaggcaaag ccgaagatcg aggtcagcac gcccagccgg
     1261 atcgtcggcc agaagcgctt gacccagctg tcctgtccgg cgtccgcctg ttcggattgc
     1321 acgaacagat agatggaata gtccaccgcc tcgccgatca gtgcggtgcc gaagcccagc
     1381 gtgatgccgt gcaccgcgcc aaagcccagg ctgacggcgg ccacgcctgc cagcacgccg
     1441 ctgaggaccg gcaggaaacc cagcgccagc gcggtcgccg agcggtacac cagcagcagc
     1501 agcgtggcaa tgatcaccac gctgatcagc gagaggcggc tgacctggct tttgatggct
     1561 tcgcgcgagg tgaccgaaaa gacgccgggc ccggtcatca ccagcttcgc ggcggtgctg
     1621 tcctggcgtg ccgtgtcgaa ggcctggcgg atggcggcca tggcgctttg ctgggcgtcg
     1681 gtgtcggacc cgtcggcgcg tgtctgcatc agcagcaggg cgcgcgcacc gtcgcgcgag
     1741 gcccaggcgc cgtcgatcag gcgcggctgc gtgccggcgt cgagctgccc gagcaattgc
     1801 accatttcgc cggtcggatc gcgcggcagc agggacttga ccatcagccc ggccggcgag
     1861 gccagcaggt ctatgctgtc ggccagggct gcgtgcaggc cgtcggcgct gaagcggtcg
     1921 ggtgtgacaa gcgggctgag caggtagcgg ttgttgaaca ggtaagtccg gtcgcgctcc
     1981 ttgttgaccg gctctccgtt gttgacggcg acaaaagccg ggtcggcgcg cagggtgcgg
     2041 gcgacctctt tggacaggcg ggcgcgggct ggcccgtcct ccccctcgat gccgaccagg
     2101 atcaggcgcg aggccaggcc gtccttgagc tggtccatca gcagctgctg ttcggccgtc
     2161 gggttgcgcg gcatgaaggc cgacaggtcg gtggtgatct gggtgcggct gatgatggcg
     2221 ccgcaggcca gcaaaaaggc cagccacaac cagaccggca gaagccgacg ctggagaaac
     2281 cggctcattt cgcggccagc ttgtcgatca ccatgaccga gcggtccttg tcggtctgct
     2341 cgatttcaat ggtcctgacg tcggcttggc tgccggcgat gcggacgcgc tgcaccactt
     2401 ccagcatttt tgtctcgatg gggcgcaggg tcagcgtcca gcgctcctcg gaaccttcga
     2461 ggtcgaggtt gtagacgcgc cgcagggcga ccatgtcgcc ggccagggtg ccgcgtatgc
     2521 tttcggtgaa ggccaccagt tccgggtagt cctgcaggcg cagcaccatg cggcgcttgc
     2581 cgcgttcgac ggtgaggctg ccgccttcga tcagcagcga ctccggccgg ggcttgagtg
     2641 tgcgtttttc cagccggtcg ggcgcggtgt agagcagttc gccggacgac tccacggggg
     2701 cctcgagcag ggcgatgtat ttcttttcga caaagctggc gcggccggac ttgttttttg
     2761 ccaagccctg catcagctgg gtcacatccc aggcggcgaa ggcggggctg ccactgaggg
     2821 ccagcagcag gccggcgccg gccagccagg tttttcgtga aggggtggtc aggcggggta
     2881 catcagtcat ggggtgggac cgggttgggg cgcagctgcc ggtttgccgg cctgccagaa
     2941 attgaaaaaa ttgaaccagt tgtagggcgc cgcgcgggag tgctgctcca gcaactcggc
     3001 gtaacgcacc atcgcggcct gcaccgccgc ttcgcgcccg cctgcaggaa tggtggaaaa
     3061 atcggccagc gtttcaaaat ggatatcgta gcgattgccg ccgccataca ggccgaccat
     3121 gaacagcacc ggccgccgca ggatggccgc catgcgaaac ggtcccacgg gaagctcggc
     3181 cggctcgccg agaaagggca ccggtcgcgt ggtgtcccgg ttcaggcagc ggtcggccag
     3241 catgccgacc acgctgccgg cctcgatcag ttcatggacg cgcaacatcg aatccgcgtg
     3301 ccccagtgcc acgatgtcct gctgcgccgc cgggttgatg gcgctcaggg cggcattgat
     3361 cttgcgggcg ttttcctcgt acatcaccat ggccacgcgc aggcccggtt gccgccggcc
     3421 gaccgcgcgc aacacctcga aactgcccac atgcgcgccc atcagaaaca cgcctttgcg
     3481 gtccgcggtc aattcggcca cgaggtgttc ctggtggatg cggatgtcga aaaggttgaa
     3541 gcggtcgttg atcagataga cccggtcatg gatgacggag gcgaagctga aaaaatgccg
     3601 gaacacatgg cgccagccgg cccgtgctcc tgccggcagt gccagcgcgc ggcgcaggta
     3661 gtcgcgtgat gcgcggcggg cggacgcgtt ggccaccaga aaataggcgg caatgccgta
     3721 cagcacgcag cggcccgcgc tgcgccccag gcgcagggaa atccaggtca tcacgcgcag
     3781 catcagcatg ttgctgcgct ccgggcgatc ccgccaggcg gcctggcgac ccgcgctgga
     3841 cggacctgtg ggcgcctggc tcatggggca ctttcgaaga taaaggtgcc ggtggcgacc
     3901 tgccggccat cgccggaaat gtcaaaacgg gtgtggcccg caggggtcgg cgcgcaggaa
     3961 atcgtcagtg tctcccccgg cagcaccggg ctgaggaatt tggccgaggc gatctggcag
     4021 cgcagcgcca ggtcccggtc gtcccctgcg cccatgcgcg cctgtccggc ggcgtgcagg
     4081 gcggcatcga gcagcagcac gcccggcacc atgggcctgc ccgggaagtg gccggcaaaa
     4141 gcggggtggt cggcggcaaa acagaccgtg gtggcttcac tctgcacggg acgggccttc
     4201 ctgggcggcg ggactctggt ggaagcgggc cagcgcctcg aggacatcgc gcggcagctt
     4261 gccggtggcg ttgcgcggca gggcgtcgag caggaccagc gggcgcggca ggaagatgct
     4321 gtctatccgt tccttcagcg cggcctgcac ggcggctgcc gtcatgtcgg gggccacgac
     4381 aaacgccacc agccgcacca cggcttcgtg gccgctgtcg tcaggcatga aaaacgcgcc
     4441 gtcgagcacg ccgggaatgc tgttgagatg atggttcagg taggccagtg agttgcgctt
     4501 gccggcgata ttgaccaggt cctcgaggcg accgtgcaac agaaaatggc ggttgtcgat
     4561 ggcctgcagc acatcgttga gcggcaccac ctgttcgaca tggccgccgc aggcccacat
     4621 gcggtcatcg tgcggtgtca gggttaccag gggaaaaagc tcccactcgg cggtttgcgc
     4681 ggttcgccgc gatgcgatct ggccggtttc ggtggaaccg taaatctcga acagcggggc
     4741 gtcaaaacgg gcttcgcaac tttgcgcgag ctgcggggcc atgggtgccg tggccgacac
     4801 cacgaggtcc agcgcgggca ggtcgatggc cgaggccagc agcacgcgca gatgcacggg
     4861 ggtgctgacc agcatgcgcg ggcgcgtgac ggacttgacg gcgctgacga tgtcggccgg
     4921 gtaaaacggc tggccggcgt gtaatgcgcc gccgctttgc atgggcagca ggacggtgga
     4981 ttcgaagccg aacatgtgct ggggcgggac cgtgccgacg atggtgcagg gcgcggcttc
     5041 gtccaggccc agccgcagcg cctcaccgcg ggcgctgcgc accagcgagc cccatgtctt
     5101 gcggtgtggc agcggcatgc cggtcgaacc cgaggtgaat acgtcggcga ctgcctgttc
     5161 gccggggagc agggggatgt cgcaaggggc ttcaggatcc gtgtgggacg gtccgtgggc
     5221 aaacgcatcc tggtaggcaa aaccgggcag ttcgatcgcg tggggggcat cggccaggca
     5281 gaacacatcg ggcgccaggc tgcgcagctg gcgcatcatt tccggggtgt gggtgggggg
     5341 cagcaggctg atcttctgtg tcagcaaggc ggcaccgagg ccgaccgcga aatggtagcg
     5401 gtcgctgcag gcgttgagca tgtgtttgcc cggcggcagc gatgcggcca gctgtttcac
     5461 atcgcccagg tactggcgga ccgtgatgat ctccccatag cgccaggcca ggatgtcgtc
     5521 accacaggtg tggctgatga gcgcgtaggt ggtcatgggc tgggatgaag ggctgggagc
     5581 ttgggcgggc aaaggtagtg caggagcgcg gcgggccgtc aggacgaggg cggcagcccg
     5641 cccgcgctgg cctggcggta ggcgcgaatt gcctccagcg gaccggcccg gtcggacgga
     5701 gccagcagca acaggcgcgc cgcgtattcg gcggaaaaca tcacgaccag cagcggcagg
     5761 ttcagcaggt tggcaaaagc cgaccagacg ctgaccggcg cgagccagaa cagcagcagc
     5821 gacagcgtgg tcatgacggt gaaatacagg gtccaggccc aggtcacctg ccgggtgtag
     5881 cgggccaggg cgggcgacag gctgccgtgg atgatggcgg caaaacgcga gaccatgggc
     5941 tgctggccgg cgcgcagggt acgcgcgaag gccaggccca gcagcgcctg tatgccggca
     6001 tgctgcagca gaaacaccca gttgtagtgc aacacgagcc agccgctcac cgcatacagc
     6061 agggtgcagc cggccaggca cagcatcagc atccagggcc gctgcggcga gcgccaggcc
     6121 agcacgaggg ccagcgcgat cagcggcgcg atcgccaccg ctgcgtccag caggtcgggg
     6181 tgggggtcgg ctgacgcaag atgcgccagc accgggtagg cggcgcagac ggcggcaatg
     6241 gctgcccagc ggacgccgcg cagcactttt gccgtcaagc caggtttcac ttcgttcttt
     6301 gctgcgcgat gtggtcgctc aggctgcgca gcgactggaa gatggcggtg ttgtcctcgt
     6361 ggtctgcgcg cagctgcagg ccataacgct tggacaggac cagcgcgatt tccaggatat
     6421 cgatcgagtc gagccccagg ccctcgccat acagcggctc atcgggcagg atgtccgcgg
     6481 cagcggtttc cagattcagg gaagtgacca ccagttcggc cacttcacgc atcagcgcag
     6541 catcggctgc aggcgcggca gagggttgga gggaggagtc tggcatcgtg gttgtaggca
     6601 tggattttca gggtgtcggg gcgttgcgcc ggtgctgcca acccccgggg ttcagtgatg
     6661 ttttgatggt aactgacatt gttttttacg cgctgcatgg ctgtaggtgg ccgcccctgg
     6721 agtgtgtcag accgaaacga aacaagacgt aaaaaatgcc cccgaaaacc cgatggcagc
     6781 cggccttggt ctagccggcc tcgctgtcac cggatttgac ggcccgcggg gcgcggggca
     6841 ccgacagggt ttgggccggg gtgggcgcgg cggtctcggc aggcagggcg gcatcaagca
     6901 cggcctggcg cacgctggcc ttgtcgccgg cgcctgcaaa tttgctgtac ttgccaagcg
     6961 tggtcaccag ctggccgtaa atgcgcgggt tgccggccac acattcgccg tggtccatga
     7021 agtcggattc gccggtgaag ttgccaatca ggccaccggc ctcggtgacc agcagcgagc
     7081 cggccgccac gtcccagggc tgcaggccgc tttcaaaaaa gccgtcgctg aaaccggcgg
     7141 ccacataggc caggtccagg gcggctgcgc ccgggcggcg cacgccggcg cactggctca
     7201 tgacttcgcc gagcgcgttg aggtaggtct tgagcttgtc gccgggacgg taagggaaac
     7261 cggtggagat caggcattcc tgcaggcgcg tgcgtttggc cacccggatg cggcggtcgt
     7321 tgacgtaggc gccgcggccc ttgctggcgc tgtagatgtc gttgcgggtg gggtcgtaca
     7381 ccaccgcctg ctcgaccttg ccgcgcacgg tcagggcaat gctcacgcag taaaacggga
     7441 aaccgtggat gaagttggtg gtgccgtcca gcggatcgat gacccagacg aattcggaat
     7501 ccctggcgcc gtgttcgctg cccgactctt cggccaggat gccgtggccg gggtaggcag
     7561 tcagcagcgt ctcgatgatg atcgcttcgg ccgcatggtc gacctcggtg acgaagtcgt
     7621 tggtctgctt ggccgagaca cgaaccgcct ccacatcaag cgcagcacgg ttgatgatgg
     7681 cgccggcggc gcgtgcagcc ttgacggcca cattgagcat ggggtggaga ttgctggaca
     7741 tatagggcta agaaagagcg gaagggctgc ggcccggtaa ccggggcagg cgacaataac
     7801 gccattttac cggctcgggc cggttacccg gctttttatc catgaaaacg cgctttatcc
     7861 tgatcaacac cagccacgcc ggcaacgtcg gcgccaccgc ccgggccatg aaagtcatgg
     7921 gcttcggcga cctggtgctg gtggcgccgc gctgggacaa tgtgctcagg cgcgaggaaa
     7981 ccatacagcg ttcgagcggg gcgctggacg tgctgaagaa cgcccgcatc gtcgcaacgc
     8041 tggacgaggc gctggacggc atgacccatt tgtgcgccac ggccatgacg ccgcgcgact
     8101 tcgggccgcc cacgcgtgcg ccgcgcgagc acttcgcggc cctgctgggc ggcgccggaa
     8161 tgctatcaaa tcaggagctg actgcgcccg tcgataaggc gttggaggcc gatttggctt
     8221 caaaacccga aggcgtggcc tttttgttcg gctccgagcg ttttggcatg cagaacgagg
     8281 acgtctaccg ctgccatgtg gcgctgagca ttccgaccga tccgaagttc ggctcgctca
     8341 acctggcggc ggcagtgcag ctgattgcct acgactggcg cgaggcgctg ggcggttttg
     8401 gcgtgcaggc ggccacgccg gaaccccggc tggccgatgc cgccgccgtg gccggcatgc
     8461 tcgggcactg ggagcaggcg ctggccgaca tcggctttct ggacccggcc tcacccaaaa
     8521 aactgatgcc acggctgaac cagctgttca accgcgcgca gctgagcccc gaggagattc
     8581 acatcctgcg cggcgtggcc aaggccatga gccagaaagc ccacgaagct ggcgccagcc
     8641 tgggtgcgcc agcagagtca gccgccaagc cataaactcg cagcatgttc tccagactcc
     8701 gctccgacat ccagtgcatt cttgagcgcg acccggccgc gcgcagcggc tgggaagtgc
     8761 tgacctgtta ccccggcctg cacgcgatta tttttcaccg gggcgcacac tggtgctgga
     8821 cccacgagct caaatggctg gggcgcttca tctcgcagat cgcgcgctgg ctgaccggca
     8881 tcgagattca ccccggggcc atcatcggcg agcgcgtgtt tttcgaccat gccatgggtg
     8941 tggtggtcgg cgaaaccgcc gaaatcggtg acggttgcac gatttaccag ggcgtgaccc
     9001 tgggcggcac ctcgctctac aagggcgaga agcgccaccc gaccctgggc aaaaacgtgg
     9061 tggtgagcgc cggcgccaag gtgctgggcg gttttctggt cggcgacggc gccaagatcg
     9121 gcagcaacgc ggtggtgatc aagccggtgc cggccggcgc caccgcggtc ggtattcccg
     9181 cgcgcatcat cccgtccaaa accggggaga gtgcggatgt gacgacggcc cagaacaagt
     9241 tcgcggctta cggcatcacg ctcgatgacg acccggtgtc ccaggccatg aagggtctga
     9301 tcgacaatgc ttcctcgcag gagcaccaga ttgcgctgct ctggcaggcg atcgagaagc
     9361 tctccgacaa cgggaaggcg caccgggact gcgtgccgga cgatgcggct ttgcaggaga
     9421 cctttgaggc tgagaagctc aatcagttga tggggaagta agaggcagac agtcatgttt
     9481 gcacgtgagt gcagcacatg cggccagatg aggctccctt ctctcgccca tcggggcgag
     9541 ggaagggcgg ggggttggga gtggggagtt acgcctactg tccgggacaa gccgcagaag
     9601 cagcgcaatg caaatagtgc tattaagttg atagctgcct gcgcctgcca gacgggagct
     9661 gaaggctaat ttgatgctaa atcaggcgga agtcgcccga atcgccgact tggggcggaa
     9721 ggccttgcag accgcctcgt cggtttccag gtaaggtccg ccaatcaggt cgacgcaata
     9781 cggcacggca gcaaaaatac cggcgactgc ctgcgagccg tcggcatttt tcaggccttc
     9841 gagtgtttcg gcaatcgcct tgggctggcc gggcaggttc aggatcaggc tctggccgcg
     9901 gatcacggcg acctggcgcg acaggatggc ggtgggcacg aatttcaggc tgatctggcg
     9961 catctgctcg ccaaaccccg gcatttcctt gtggccgact gccagcgtcg cttcgggcgt
    10021 cacgtcgcgc agcgccgggc cggtgccgcc ggtggtcagc accagcgagc agccggcatc
    10081 gaccagctcg atcagggtgg cgctgattcc cgcctgctcg tcgggaatca ggcgctccgc
    10141 aaattcgatc gggttgtgca gcgcgcggcc cagccactct ttcagcgcgg gcaggccctt
    10201 gtcttcatag acgccgctgg acgcccggtc gctgaccgag acgatgccga tcttcactgc
    10261 atcgtgggcc atggtcaagg cgcctccggc gccagcgtct gccgcaccag ctggaagatc
    10321 tcgcggtagg acttgccgtg gcgaggcgct tcgcccggtt tttcgggctt ggcgtccttg
    10381 cgggcctggc gcaccagggc gcgcagctgc tgcgaatcgg tgtccgggtg ttctgccagc
    10441 cagtcgccca gcgcgtcgtc ggaggcgatc agcttgtcgc gccactgctc ggccaggtgc
    10501 agcgccagcg tgagctgggc ggagccgttt ttctgctcgt caatcgccgc gcgaatcggc
    10561 tcggtgtcca gcgggcgcat cagcttgccg atgaactgca tctggcgccg tttgccctcg
    10621 aaattggtga tttttttggc ctggacgatg gcgtcgagca gtttttcggg caggtccagc
    10681 ctcgccatca ggtcggcgcg cagggtgagc agggcttcgc cgagggcctg tttttcgtcg
    10741 gcttcggcct tgagctgggt cttgctgggc gggcccactt cttccagggc aatttcttcg
    10801 ggtttcacga agcggccgtg ggaccagtag cccttgatag ccttgggagt cttgttgttc
    10861 atagcagtca gtatcatagc tacccatatg accctcaaga cttccaagac atcctccggc
    10921 gccaaacagg cggtccggcc ttcttcccaa ccggaaaagg gcttcagtta ccaccgcgat
    10981 tttttcgagg acctggtgga cagcgccctc gcgcacgcca agaaaatcgg tgccaccgat
    11041 gccgccgccg aagcttccga aggctgcggc ctgagtgtca gcgtgcgcaa gggtgaactt
    11101 gaaaatgtcg agcgcaaccg cgacaagtcg ctgggcatca cggtctatgt gggccagaaa
    11161 cgcggcaacg cctcgaccag cgatttttca aaggcagcga tcgagcagac cgtgaaggcg
    11221 gcctacgaca tcgcgcgttt caccgccgaa gacaagacct ccggcctgcc ggccgaccgc
    11281 gacatcgcgc gtgtgcaccc tgagctggac ctgttccacc cctgggccat cgactcggaa
    11341 ggcgcggcgc aactggccct gcaatgcgag gcggccgccc tgggcaccag caaacgcatc
    11401 accaatagcg aaggcgcggg tgtgtcggcc cagcaaagcc attttttcag cgcgcataca
    11461 cgcggtttcc gcggtggtta tgccagctcg cgccacagca tctccgtcgc gcctatcgcg
    11521 ggcaagggca gccagatgca gcgtgatgcc tggtacagct ccatgcgcga cgccagcgaa
    11581 ctggccagcc ccgaggccgt gggccgttac gccgccgaac gcgcgctggc gcgcctgaac
    11641 agccgcaaga tcaagaccgt gcagtgcccg gtgctgttcg agtcgccgct ggccgccggt
    11701 ctgctgggcg gttttgtgca ggccgtcagc ggcggcgcgc tgtaccgcaa aagcacgttt
    11761 ttgctgaaca gcctgggcaa gccggcgttt cctgcgcaca tcgacatcag cgaagacccg
    11821 cacctgcgca agggcaaggg tagcgcgccg tttgacgaag aaggtgtgaa aacgcgcgca
    11881 cgccaggtgg tcgaggccgg catcgtgcgc ggctactttt taagcacgta ttcggcgcgc
    11941 aagctcggca tgcgtaccac cggcaatgcc ggcggatcac acaacctggt gctgagcagc
    12001 cgccagacga aagccggcga cgacctcgac gccatgctga agaagctggg aaccggcctg
    12061 ttcgtgaccg agctgatggg ccagggcgtg aactacgtga ccggcgacta ctcgcgcggc
    12121 gcctcgggtt tctgggtcga gaaagggcag cttgcctttc cggtggaaga aatcacgatt
    12181 gccggcaacc tcaaggacat gctgatgggc atccaggcgg tgggcgccga tgtctacaac
    12241 tacggtgcca agagtgtggg ctccatcctc attaaccgga tgaagatcgc cgggagttaa
    12301 agcccccacg gttgctcact gcgtgtagcg ccctgccccc cgagggggcc g
//
LOCUS       DAIDFG010000060        12050 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_60_length_12050_cov_18.864360, whole genome shotgun sequence.
ACCESSION   DAIDFG010000060 DAIDFG010000000
VERSION     DAIDFG010000060.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 12050)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12050)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12050
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60_length_12050_cov_18.864360"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1984 a         4062 c         3830 g         2174 t
ORIGIN      
        1 cgagctatgc gcaggaacag gaaaaaatca ccgaacgctg gccggccgcg gtgaagttca
       61 tccagcagca ccagctcaac gaattttttg ccgagggcgc caaagacttc ggcatcgtca
      121 tgcagggcgg cctgtacaac agcgtgctgc gctcgctcga gctgctggga ctggccgatg
      181 cgttcgggca gtcgcaggtg ccgctgtatg tgttgaacct gacctacccg ctgatcgacg
      241 acgagttcaa gcgtttttgc gccggcaagc gcggcatctt gctggtcgag gaaggccagc
      301 ccgacttcat cgagcaaaac gtcaacacca tcctgcgccg ggccgacatc cagacccgcg
      361 tgcacggcaa ggacgtgttg cccatggccg gcgaatacac cggcggcgtg gtgctcaagg
      421 gcctggcccg cttcataaac atctacgcac ccgagctgct ggagggccgg gagctgccgc
      481 tggcggcgcg ccccgcggcc accggcctgg cggtccttag cggcaacccc aaggcctttg
      541 cgatccaggc ggcctccgcc gaggtgcagg cccgcccgcc ctcgttttgc accggctgcc
      601 ccgagcgccc catcttcacc gcgatgaagc tgatcgagcg cgagctcggc cagcaccatg
      661 tgagctgcga catcggctgc cacctgtttt ccatcctgcc gcccttcaac atcggcgcca
      721 ccaccatggg ttacggcctg ggctgggccg gcgcctcggc cttcaacacc actgaaacct
      781 ccaaacgcac gatcgccatg atgggcgacg gcggcttctg gcacaacggc ctgaccagcg
      841 gtgtcggcaa cgcggtgttc aaccagaccg acaacgtgct gctggtggtg gacaacggct
      901 actcggccgc caccggcggg caggacgtgc tgtcgtccaa ggccgtcaac ccgctgcgca
      961 gcaccaacaa cccgatcgag aaagccgtgc gcggcgtcgg cgtgaaatgg gccaagacgg
     1021 tgaccaacac ctacagcctg gcgcagatgc gcgacaccct gcgcgaagca ctcaccacga
     1081 aagaaaaagg ccccaaggtc atcattgcgc aaagcgaatg catgctgaac cgccagcgcc
     1141 gcgtcaagcc gcagatccgc aagatgattg ccggcggcga acgtgtggtg cgcgaacgtt
     1201 tcggcatcga ccccgacacc tgcaccggcg accactcgtg catccggctc tcgggctgcc
     1261 cctcgctgac catcaagccc aacccggacc cgctgcggcg cgacccgatc gccacggtga
     1321 tcgacagctg cgtgggctgc ggcttgtgcg gcgaagtggc acacgccgcg gtgttgtgcc
     1381 cgtctttcta tcgcgccgag atcatcaaca acccgaccgc gtgggacggc ttcaagctga
     1441 agctgcgcac ggcggtgatc ggctggctgc aaaaccgcat tgagcgccgg ctcgaaggga
     1501 ttgcggcatg agtacaccaa tgaacagtga gcgagccatc acgatcgcca tcctcgcgat
     1561 gggcggcgaa ggcggcggtg tgctggccga ctggctggtg gatctggccg aacagaacgg
     1621 ttacttcgcg cagaccacct cggtgcccgg cgtagcacag cgcaccggcg ccaccgtgta
     1681 ttacctcgag atgttccccg aagccgccgt tcccgccggc cgcatgccgg tcatggcgct
     1741 gagcccggtg cccggcgagg tcgacatcgt gattgcctcg gaactgatgg aggccggccg
     1801 cgcactgcag cgcggcctgg tcacggccaa ccgcaccacc tttgtggcct cgacgcaccg
     1861 cgtgtattcc atgaccgaac gcaccgccat gggtgacggc cgcgtcagct cggacaggct
     1921 gcttgaaggc gggcgtgccg cggcccggca tttcatcagc gccgactttg cccagctggc
     1981 cgaagacacc ggcagcctga tcagccccac gctgtatggc gcgctggccg ccaccggcca
     2041 cctgcccttt acccgcgaac agttcgaagc caccatcacg cgcggcggcg tcggcgtcac
     2101 ctccagcctc aaggcctttg ccgcgggttt tgccgtggcg cagcaaacgc tggcgccacc
     2161 agcggcggcg gccctgccag ccgctgcgcc catgccgccg gtcggcgcgc ggctcgcggc
     2221 gctggcgcag cgtattgaac aaaactttcc ggccagcagc cacgccacgc tggccgccgg
     2281 cgttatgcgt gcggccgact accaggacgt gccctacgcc gcaacctacc tggaccgcct
     2341 gcagccgctg ctggcccgcc tgccggccga ccctgcgctg gccgccgagc tgctcgacga
     2401 aaccgcgcgc cacctggcgc tgtggatgtc ctacgaagac accgtgcgtg tggccgacct
     2461 gaaaacccgg cgctcgcgtt ttgcgcgcgt cggtggcgaa gtcaagctga gcaatacgca
     2521 gcacctgggc atcaacgagt ttttgcatcc gcgcgtcgag gaaattgccg acacgctgcc
     2581 ggccggcctc gggcgctggc tgctggccac gccctgggcg cgcgcctgcg tcgagccttt
     2641 cacgcgcaag ggccgcatcg tgcagaccag ctcgatacgc ggctacctgc tgctgtacac
     2701 gattgccgcg ctgcgtccgc tacgtccgcg ctcgctgcgc ttccaggtgg agcagcagcg
     2761 catcgacggc tggctggcca ccatcacaag cctggcgagc acgcagccgg cgctggcgct
     2821 cgaagtggcg cgctcgcagc ggctggtcaa aggttatggc gacacccatg tgcgcggctg
     2881 gcgcagtttc gaacgcgtca tgagcaaact gccgcaactg caggccggac ccgatggggc
     2941 ccggaaattg caggcactga gccaggccgc gctggccgac gacagcggca aggcgctgga
     3001 ccagatgctg gccacggtgt aggtcatcaa cgtgagagtg gtagtcgccc gcattgacgg
     3061 gtgtttgtgc agagcggatc atcaatagca aatccaacag gagacagacc ccatgaaagt
     3121 cagcttttta aacaccgtca aaacggtttc ggccgtgctg gtcgccgcca gcttttcggg
     3181 cggcgtcgcc gcgcaggaag tcacgctgcg cctcgtaagt gcgtttgccg aaaacggcat
     3241 ttacgtgcag cgcctgcaac cctggatcgc caaggtcaac gccgaaggca agggcgtgct
     3301 gcagatcaac ttcctcggtg gcccgaaagc catcccgacc tttgaagccg gcaacgcggt
     3361 gaaaaccggc gtggttgaca tggccatgaa caccggcgcg ttctacacca acgtcatgcc
     3421 cgaggccgac ttcctcaagc tcacgcagat ccacgtggcc gagcagcgca agaacggcgc
     3481 cttcgacgcg atcaacaagg tctggaacga gaagggcaac atgcagtacc tggcccgcat
     3541 ggtcgagaat cagccctttc acatctacac caacaagaag gtcgacaagc ccgacctgac
     3601 cggccagaag atccgcatca ccccggtgta ccgcgacttc ttccagtcac tcaacgccaa
     3661 cgtcatcacc acgccaccgg gcgaggtcta caccgcgctc gagcgcggcg tggttgatgg
     3721 ttacggctgg cccatcggcg gcatttttga cttgaactgg cacgaaaaaa ccaaattccg
     3781 catcgacccc ggtttctacg acgccgaagt ctcgctgatc atgaacctgc cggcgtacaa
     3841 aaagctcacc gacacgcagc gcaactacct gcaaaagcag ctgctcgtgc tcgaagccga
     3901 aaacaccttc tgggccaagt acaccacctc ggaaaccgca cgccagcagg ccgccggcat
     3961 ccagaccatc acgtttgatg ccgccacggc caaggccttc cgcgacaagg cttacgacat
     4021 cggctgggcc ggtgcgacca agcagagccc ggagattgcc gcccgcttca aggcgctgtt
     4081 ttccaaatga ggtgccagcc gcagccggcg catggccgcc tcacccgcct gtgccttcgc
     4141 tgcggtggct tgattcatga ttgaccgcct gtcgtcccac tacggcaggg cgctcagcgc
     4201 gctggccatg ctcggctgcg ccatcctgat ggccatgatg ctgatcatcg tggccgatgt
     4261 ggcgctgcgc aacctgccca ttcccggcct gccgcaaggc ctggcctgga gcaatgaaat
     4321 ctccgagctc atgctctacc tgatcaccat gtgtgtggcg ccctggctgc tgcgccaggg
     4381 ccagcacatc cgggtggaca tcctgctgca ggcgctgccg ccgcggctgg cctggaccct
     4441 tgaatgggtc ggcgacctga tcggtctcgc ctgctgcgtg gtgattgcct ggtacggcac
     4501 gcaggccgcg tggtcgagct acatctcggg cgccgtcaac atcaagaccc tggtcacccc
     4561 cgagtggtgg gcgctggcgc cgctgccgct ggtttttgtt cttcttggaa ttgaaatgat
     4621 ttttcgcatg atccggctgc aacgcggccc gcgcggtccg cgccacgacg cggtgagcgc
     4681 agcatgagcg gctggggcct ggccgcctgg ctgatgctgg gcggctccac ggtgctgctg
     4741 ttcatcggca tgccggtggc gctgaccttc atcaccatca acatcattgg cgcctggctc
     4801 tacatgggcg gcgagcccgg actcatgcag ctggcgcgca gcagcgtgac ctcggtcacg
     4861 gctttttcac tgacgccgat tccgctgttt gtgctgatgg gcgagatcct gttccacacc
     4921 ggcctggcac tcaaggtcat cgaaggcgtg gagcgcctga tcaaacgtgt gcccggccgt
     4981 cttgccgtgg tggccgtggt cgcaggcacg gtgttctcgg cgatctcggg ctcgacgatt
     5041 gccaccaccg ccatgctggg ctcgctgatg ctgcccatca tgctggcgcg cggctaccac
     5101 ccgacgattg ccaccggccc catcatggcc atcggcgcgg tcgacatgct gattccgccc
     5161 tcggcgctga ccgtgttgct cggctccctg tcgggcatct cgatttccaa gctgctcata
     5221 ggcggcgtga ttccgggtgt gattctgtcg gtggcctttg tgctgtggat catcatccgc
     5281 gtcagcatca gccccaagct cgcgccggcc gacagggacg acgtggtgca acaccgcggc
     5341 tgggcccgct ggagcctgtt ttttggctat gtgctgccca cgctgtcgat cttcggcgtg
     5401 gtggtgggcg cgctggccgc cggctgggcc accccgaccg aatgcgcggc gctgggcgct
     5461 tttgccacca tgctgctggc cctgtgttac cgcgcgctga cctgggatgc ggtgatcaag
     5521 gcgctcaagg gcacggccgg catctcgggc atgattctgt tcatcatcct gggcgccacg
     5581 acctttgcgc agatcctgtc gttctcgggc gccagcagcg gcctggtgca gttcatcacc
     5641 ggccagggcc tgtccaccaa catgattgtg gtcggcatga tgctgatgct gattttcctc
     5701 ggcatctttg tcgaccaggt cagcatgatg atgatcacgc tgcccatctt catgcccatc
     5761 gtgcaggcgc tcggcatcga cccgatctgg ttcggcgtga tgttcctgat ctgcatgcag
     5821 ctgggcctgc tgctgccgcc gcacggcctg ctcctgatga ccatgcgcgg cgtggcgccg
     5881 ccgtcggtca ccatgagtca cattttcatc gccgtcgtcc cttatgtact catgagcctg
     5941 atcctgctcg cggcggtgtt ctggatgccg gcaattgcga cctggctgcc caaactgatt
     6001 ggatagcccg cctccaggat tgctactttt ttcatagctt tgagcgcccg tcagtattgg
     6061 gccggagccc tgttttgctc cagacgcagg ccctgcgcga cctgtgggac agcgcctaca
     6121 ggccaaggcg tcccgggccc acaggtgcgg ccgccgcagt gacgtacggt taccccttca
     6181 tcaaaagggg agccatgctg ctcagcactt tccttgtcga agacaagccc gagatacgcg
     6241 acaccgtggt cttggccatg gaagaagtgg cgccgctcaa atttgtcggc catgccgcca
     6301 gcgaggctgc ggcccgccaa tggctgcgca ccttcaatga cgactggcag ctggccattg
     6361 ttgacctttt cctctcggaa ggttccggct ttggcgtgtt gcgcgactgc caggcgcgca
     6421 agccgaacca gaaggtggtg gtgctcacca gttattgcca tcggaacatc acggaccgtt
     6481 gcctgcagct gggcgccgac cgggtgttcg acaaatccgc cgacctggaa gaactggtgg
     6541 cgttttgcaa ggcgcacgcc aacaacctgg cccgcgtggt accgcaggtg gaatccaacg
     6601 gcacgctgca ctgagcgccc cgccccctgc gggtgaaggc ctgccatgac gtcgcaggtc
     6661 gtgtaacctc accggcatga ccgccgccgc accgccaccc cacgccgccc acgcgtcgcc
     6721 cgcccttccc gacccccgcg ccgatcccac gcgcgccatc tgggtcatgc tgtttgcgct
     6781 gatcaccgcg tttgcgctaa gccaggcctg ccgcaccgtg gccgccatca tggccacacc
     6841 gctgcaggcc gactttggcc tgagcgccca ggcgctgggc ctgtttgccg gcgcctttca
     6901 ctttgccttt ggcgccatgc agctgttcat gggcataggc attgacctgc acggcgtgcg
     6961 gcgcaccgtg ctcaccgcgt ttccggtggc gattgccggc gcggccgtgt cggcgctgtc
     7021 caccagcttc ggcatgctga tggccggcca ggtgttgatc ggcatcggct gcgcgccggc
     7081 ctttctggtg tgcacggtgt tcatcgcgcg gcacatgccg gcgcaccgct ttgccgccat
     7141 ctcgggcctg gccatgagcc tgggcggcat cggcctgctg ctgaccggca cgccgctggc
     7201 ctggctgatc gaggccagtt cgtggcgcat gggtttctgg gtgctgggcg cgtggggcgt
     7261 gctcgcctgg gggctgatct gggcgctggt gcatgagccg ccgcacccga tggatgcgct
     7321 gaaaaagaag gagtccgtgg gccaggccct ggccggcttt ggcgcgctgt tcacgctgcc
     7381 gcacacctgg ggcatcttgc tgctggccag cgtggggtat gcctcttttg tctcgctgcg
     7441 cggcctgtgg ctgggcccca tgctgatcga gcgtcatggt ttttcgctgg tgcaaagcgg
     7501 caacgtggcg ctggtgatgt cactggtgtc gctggccggc ccgccgctgt tcggccgctt
     7561 cgaccccggg cccgccacgc ggcgccgctg gatcaccggc ttcaccttgc tggtggccgg
     7621 cctgtttgcc acgtttgcct tcatacacag cccgctcatc gacgtggcgg cgcccatcgt
     7681 catcggcctg ctgtcgggtt acatgattct tcaatattcc gacgtgcgcg ccgcctaccc
     7741 ggcggccatg acgggccgcg ccctgtcggt cttcaccatg gcgatgtttc tgggcgtggc
     7801 caccatgcag tggttcaccg gcgttgtggc ctcggtggcg tcggctcagg gcgtggagcc
     7861 tttcaccgcc gtgctggcca gcattgccgc gctgctgacg ctgggggcat tggccttcac
     7921 cgtgctgcca cagccggcgg cgccggccac ccacgcctga gctagccggc cggcgctgcg
     7981 tcaggcgcca ccaccggcag cgcgaaataa aaacggcgct cggggccctc ggaagccggc
     8041 cgaccgacgc tcctgtattc atagctgctg gcgcacgctg cataaaggct gcagcctgtt
     8101 ttaaccatca ccgtcgcggg gccacggccc ggggtcatgg cagcgtggct ttgggctggt
     8161 tggagcgcgc attgacctgc ggcgcgctgg tttcctggcc cccgggtggc gttctcgtgg
     8221 gcgtgctggc tgccggtggg gtcgggaatc tgaaccacct tgccctgctg ccaggcgtcg
     8281 gcccccgggt ccatggtcgg tgacgcgatg tcctcgcggc tctttttgcg ggctgcgctg
     8341 tcgatgccga agcgcttgtc gcgcgcggcg accatggcgg ggtcggcctg cccgtctgcg
     8401 gtgaaaccca tcatcagttg cggcacaccc agcggcaggg ttttctgcgc gtcggtgtgc
     8461 caggtgtgcc aggtcttgcc gtaggtgccc accagctttt tcatcaactc cttctcggcg
     8521 atctccggaa tgccgggcgc cagcagctgg ccggacttca cctcgtgcac atggctgtgc
     8581 cacagcgcct tttcttcggg cggcagcccg gcaaaaagcc cggcgctgac gatgtattcc
     8641 acacccatga tcttggcgtc cttctggttg ccgtcgtaga tcacgcactg ggtcacgtcc
     8701 tcgttgagca gcgaacagta gtggtgcgcc tcgatctgca tgtccgggcg gccgttgtag
     8761 aagtgaaagc cgtccaggta ggcattgagt gcctcgaccg gcggcttgtt ctgcagggcg
     8821 atggcccccg cttccaggac ctgggtcttg atggtttcgg gctgtcccgg cgcctggaca
     8881 ctggacttgg tcctgttgtc accacatgcc cccagcgtga agccaagcaa ggtcatggcg
     8941 cttgtcagaa ccatccgttt catgggttct ccagtggcgg cacggtgatg acgaccctca
     9001 ggcctgcgcc gccatttcgc ggcaggcctc ggcgcagtgt ttgcacgccc gcgcacaggc
     9061 ctggcagtgg tccatgtcgt gttgcacgca ttccgcggcg caggcctcgc agatctctgc
     9121 gcacagcgcg cacacggccc gggcgtgctc gctgtcgcgc gccatcagcg cagcggcggt
     9181 ttcgcagatc gccgcgcagt ccatgtcgag ggcgatgcag cgcgccatcg ccttcacatc
     9241 cggctcgcgc aggcaggacg ccgcgcaggt attgcaggcg gcagcgcagc tgtagcaggc
     9301 atcgatacag cgctggtagt tctggtcagt catgggggtc tccttgggtg ggggcaaagc
     9361 gtttcggcgc aggggccgcg atgggtcttt ggtcacatta ggagcctgcg gcccaaggaa
     9421 ctgtcagcga tggtccggcg tccttgtagg acgacccggt ggcgcgaccc ggtggtgctc
     9481 tgcgccggcg gcggatgtaa aaacgcgttt catcgtttgc ccccggccac cgcccgcgct
     9541 aaggtgttct ttccctcaac catgcttcag cgcggcgcag cagcagcccg ccgtgcagct
     9601 tcagccatga cccaacagga aaagaaacag tcgctcaaca tcggcgccgg cgtgatgatc
     9661 ggcgtcatca tcggcgccat catcgaaaac ctcaccatgg gcataggcat cgggattgcc
     9721 atcggcatcg cgctgtcact ggtgaacaag ggcggcagcg cgaagtagcc cggcccggcc
     9781 ggcgcgggca aaaccgccgc cggcatcagc agcaagcctt gacctacaga cgtgtgcacg
     9841 gctgaggggt aaggtgggca agtcgttcca gcccacacgc cacccttcgc acctgccccg
     9901 ccatgaaact caaagccacc tgcaccctga ccgtgaacac ggccgccgac tgtcccatcg
     9961 tcgccatgct gcgcccgcgc agcggccagg cgcagtggat cgtcagcgac cgttatgagc
    10021 tgcagccctg ggtgccgacc accgagtatg tcgacagcta cggcaacctg tgccagcgcc
    10081 tggtgctgca ggccggcgaa atgcgcatcg aggtcgaagt ggtgatggag gtcgaggatc
    10141 agatcgccgt gcagcccgac gcgccgcaca cgccggtggc cgagctgccc gacgacgtat
    10201 tgctctacct gctgcaaagc cgctactgcc cgtcggacaa gatgtccgag aaggcccgcg
    10261 agatcgtcgg cgacacgcca cccggttatg cgcaggcgga aaccattcgc ggctggatca
    10321 acgccaacct ggactaccag tacggcgtga gcgacgccag caccgacgcg atggactcgc
    10381 tggaccacgg cgccggcgtg tgccgcgact ttgcgcatgt gggcattgcg ctgtgccgca
    10441 gcctgcagat tcccgcgcgc atggtcgtgg gttacctgca cgaactggac ccgatggacc
    10501 tgcacgcctg gttcgaggcc tttgtgggcg gacgctggta caccttcgac gccacccaga
    10561 aggagccgcg cggcggccgc atcgtgctgg cctatggccg cgatgccgcc gacgtggcct
    10621 tcatctccga ctacggcccg cagccgctgg aagtcagcga catgaaggtg caggtcagcg
    10681 aggtgaccga cactgttgcc gcctgacggc agcctgtggg cgcctcaaac gctctgtttt
    10741 tgatagctgc ctgcgcacac tccataaggg ctggaggcca atttcatgca caaaccgcaa
    10801 acaaacggga aagggagcgc ggctcagtcc ttgtagctgg tgtcgagccg gtccagcttg
    10861 cgcagcagcg ccggccagac aaaggggccg cccatgccac cgccgcgcac ataggtggca
    10921 gcgcggtcgt cgccggtgta gatctcttct tcaaaatgaa tcagctcgcc gccggcctgt
    10981 gcgctgagct ggatctggca ggtgctttca aagcggtaca tgttcatgaa ggcgtcggca
    11041 atcgtttcgc ccaccgtcag caggccgtgg ttgcgcagca ccagatggcg cgcgctaccc
    11101 aggtcttgct gcaggcgcgg cttctcgtcg tcgcggaagg ccacaccttc gtagtcgtgg
    11161 taacccagcg aggccagcac aaaggccgac tgctgcgaga tcggcaagag accgcacttc
    11221 tgcgcgctga cggccacacc cgcgcgggtg tgcgtgtgca gcacacacgt catgtcgtgc
    11281 cgcgcctcgt gcaccgcgct gtgaatcaca aagccggccg ggttcaccgg atgcggcgag
    11341 tcgatcacct tgttgcattg ctggtccacc ttgaccagcg acgaggccgt gatctcgtca
    11401 aacatcaggc cgtaggggtt gatcagaaag tgatgctccg gccccggcac ccgcgcgctg
    11461 atgtgcgtga acaccaggtc gctccagcca tacagcgcca ccaggcggta acaggcggcc
    11521 aggtccaaac gtaattgcca ttcttcggcg ctgacaactt ccttgagcga ggcgatgttc
    11581 atgatgatga tgatgcggtt ttcaatagat ggggaatgaa ctgactttaa tcggggcaga
    11641 caactcgcgc tggctggcta tcgcggccgg tacatgcgaa acagctgctc cggcccgaca
    11701 gtgaagtagt cgcccggccc gccgccgcgc aacacgtggc cggcgttggc cgtgtcgtac
    11761 acgccgtctt tgagcagcgc cttggcaatg tgcaccgcca ccacctcgcc cagcaccagc
    11821 caggtgtcga ccttgctgcc gtccacgcct tcgagctgca gcacctgcgt gcgccggcac
    11881 tcgaacgaga ccgggctctc gagcacacgc ggcaccgcca ccacgcgcga gggcgcgggc
    11941 gtgaggccgg cgagctcgaa ctcattgacc tcgggcggca ccgcggcgca ggtctggttc
    12001 atctgctcgg ccagcgcgcg cgtggtgagg ttccacacaa actcgccggt
//
LOCUS       DAIDFG010000061        11931 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_61_length_11931_cov_17.515914, whole genome shotgun sequence.
ACCESSION   DAIDFG010000061 DAIDFG010000000
VERSION     DAIDFG010000061.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 11931)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11931)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11931
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61_length_11931_cov_17.515914"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2234 a         3821 c         3701 g         2175 t
ORIGIN      
        1 ctggcggccg cccgggcgat cgcggccagc cgggtgtcgt cgtcgccggc aaagcgctgg
       61 tggctgctca gcgcggcctc gtccagttcg acctcgtaac ccagcgccgt caggcgccgc
      121 acgccgcgtt tgaaagcggc cttgtcgcgc acggcgctgg agggggagta gatgtagata
      181 tgttttttca cgagacgaag tatcccactg cccggaccag cccttccaaa gccgccggtc
      241 agccccccag agaacgcacc gcctgccgcg caatgctttc cccatgctcc tgcacaaaat
      301 gccctgcctg aggcaacacg aggggctcgg ggcagccgcg gatcatggcc tgcaattgca
      361 gcatgaccgg caggccgagc acggggtcct gcgcaccgac ggccatcagg gtccggcctt
      421 gccagcgttc gcgccagaaa ctgcgtgcct cccgtgaaat cgcggcaccg tccgatttct
      481 cggcctccgg caccatggcc ggaaaggcac gcagggcggc gcggtggccc cggtcgggga
      541 aaggcgcgtt gtaggcggcg cattcttcgg cacgcatctg ggggttgccg cgcgcgaaca
      601 gccgcgccac atcgaactcg gggtttttcg cgcacatctc gcgccaggcc agaaaacccg
      661 gcgacaaggg cacgtcgcca gtcgccagcg tcgtgttcat gaccagcaga ccccgatagc
      721 gctcaggtgc ggccatcggc agggtcaggc ccagcaggcc gccccagtcc tgcaccacga
      781 gaacgatgtt cttcaggtcc agccgctcca ccagctccag caggttctgc cggtgcccgg
      841 tgaagctgtg aaagctgtcc tttttgggct tgtcgctttt gccgaaaccg atcaggtcgg
      901 gcgccaccac ccggtgcccg gccgccagga acaccgggat catcttgcgg tacagatagc
      961 tccaggccgg attgccgtgc aggcacagcc aggtgaggcc ctcacggggc ccctcgtcga
     1021 ggtaatgcat gcgcaggccg gccagggacg gcaggtcgct gaggtagcgg ggtgtccagg
     1081 ggtagtccgg caggccggca aatgcgctgt cgggcgtgcg gagggcgtca tcgcgcaggg
     1141 gatggttcag ttttgtctcc tggcgctgtt cgctgcgccg ctgctggaaa aaatcctgca
     1201 gcatcgcacc gcattccccg gcaagcaccc caccttgcac ctgcgtctgg tgattgagct
     1261 gcggatgggc aaacaggtcg atgacggagc cggcggcccc ggtttttgga tccggcgcac
     1321 cgaacaccac gcgcttgagc cgcgcatgca gcatggcacc ggcgcacatg gcgcagggtt
     1381 ccagcgtgac aaacagttcg cactcgtcga gccggtagtt gcccccggcc tgggcggcag
     1441 accgcagggc ctgcatttcg gcatgggccg tcgggtcatg cccgttgatg ggcttattgc
     1501 agccggtggc gatcacctga ccatgccgca ccagcacggc accgaccggc acctcacccg
     1561 cctcggcggc ttgccgggcc tgcgccagcg ccagttgcat gtagtcggcg tcgctcatga
     1621 ttgcttcgtt tttaatagca gcttgcgcaa gtaccacggg ggctgaagcc cgatttgatg
     1681 cagattattt gtcttccggc atgggccggg cctgctgcat cgtgttttca atcgactggc
     1741 gcacctgatt ctggatctgc tggctttgct gctgcggtgt agcgccggcc gccggcgcgg
     1801 gcaaaaccag accagcgtca gccggcgcaa ctttcacagg cgccggggag agctgttttt
     1861 ttgccagtac gccgatgatg gcgaccacga tcagcaggct cagaacgcca aagatggcac
     1921 gcatgtcagg cctccttgcg gggggcggca gcgcccccca tgcccagccg ctccatccag
     1981 tcggccagcg cctgttcgtc gggcgtgccg gcggcataca tggctttcat cgcagcgtgc
     2041 gactcggtcc ggtgctggtt gagtttgagc ttgcactgca ggtctgtgac ctgcaactcg
     2101 aaagccacaa tgccggccag catcttgtgg gcaaactcct cgcccaggcc gcgccactgc
     2161 tcggcatacg gcggctcatg gtcgccaatc agttttttga gcagccggtc cttggcctgc
     2221 gggtcttcga tcaggctggc ctgcacggtg cagtgcacgg ccaggtaatt ccaggtcggc
     2281 acccgcgcca ggtcggggta gaccttgggc gacaggtagg catggggtcc gagaaaggtc
     2341 gccacggcct tcggcctggc ttctaggtag cgccaatgcg gattgggttt tgcgacgtgc
     2401 cccagcagca ccagctgatc accgcgttct tccagatgca gcggcaggtg cgtcacgtaa
     2461 ggcagccccg cgtcgtccac gctgatcagg ctggcgaagg gataggtccg aatcaggtcc
     2521 ggcgcatgaa cgccgctgtc atcattgaac tggcgaggca gatacataat ctgcgtactt
     2581 taaccgttca cggacgtgcc ggaacagggg cgatgcctga ttcttcctcg acgcagtcct
     2641 ccgcgcgttc cacccggttg cgcccttttt ccttggcgcg atacatggca cggtcagcag
     2701 cgttggtcag ttcagcggct gaggcaagct gttccgagga cgttgccacg ccgatgctga
     2761 ccgtgacatg aatgctctcc ttgtcttcga aatcaaaagc atggccctca atgatcttgc
     2821 gtaaacgctc ggcggggatc attgccgcat cagccccggt ttccggcagg atgagcgtga
     2881 tttcttcgcc gccataacga caggcaatat ccacctcacg acaggtaccc accaccagat
     2941 caccaatgct tttaaggacc agatcgccgg caaggtggcc gtagcgatcg ttgatctgct
     3001 tgaagttgtc aatatccagc agcagcaggg aaatcggttg gcccgtgcgc ccgctgcgga
     3061 caaactcttc cctgagccgc aggtaatgca tgcggtgatt gaacagtccg gtcagaccgt
     3121 cgtggtcggc gagttcgcgc agacgctgct gagcctgatc cagttgccgc ccgaccttgc
     3181 ccaccagcca gtagaaaaag cccagcaacg tcatgcaggc caaaccgcca acccacgcgc
     3241 tctcgcgagc cagtgcatcg agcgtctttg cctggagagt ttttgcctgg ggcgagatat
     3301 caaccagcgt caccattctt cccacgctgc gcccggccac atcttcgaga ggctgaatca
     3361 ccgcccgata ctcagatgtc ccggccacca cgggcaccac caccttctgc tcgccgataa
     3421 gaccgcgctc caggagccga aaaagttccg gaggtgtgtt ccctttcgac tgaatgctca
     3481 ccaccatatc gggaaaccgg ttccagtccg gcacacgtcc cagtatgcgc atgcccgatt
     3541 cccatgcctg gcggtcgagg aatttcttgt caatcaagat ggacatcttg acgttcgcct
     3601 gtcgctgtac ggccctcagc acatgatcaa tttccatccc caactcgacg aaaccgagca
     3661 gttgcccatc aggatcgaac cacggtgtga ccaaacgcag cgtgaaagta cccagcggcc
     3721 ccaactcgac accgtagctg gttttattct gctgctgtgc ctgcagggtg gtgttgcggt
     3781 ttatggtgtc gccaaagcgt tccggctgat gcacgcgcag gatattcacc cggttcgggc
     3841 cggaaaaata gagatgggtg atatcgtatt tttttctcag ttcggcaaac atgccggccg
     3901 agctctgcag caattgttca cggtcacgtc gcgccaggct ctggcgcaac gatcggtcat
     3961 tggccagcgc ctccatggcc gcccccagca tgttggccga gagttcgatc tgatcccggt
     4021 agacatgctc ggcgagcgca gaggtttcct ctgtataccg gttgatatca ccggtcgctt
     4081 tgaggcggat cagcgtcacg atgagcagcg tagcaaacac cagcacgctg gcgagcggga
     4141 gcaagaacca catccgcagc cgctgcgttt caccgcccgt ttttctcaga aagaccatat
     4201 atccccccaa gacagcaaaa aaaagccctg cgaaagctga aaccagccag ccccccgacg
     4261 gcaagactta tccctatgct atatcaccgc caggctcgct agggggctga gcgggggcaa
     4321 aagcgaaatt gagctgcaga ttcgaggaag atgcctgctt gcatcatcgc cgcactcgtc
     4381 aatcgcccca tggaagcaat ggactcgccg gacgccgcgc cctactccca ctcgatcgtg
     4441 gccgggggct tgctgctgac atcgtaggtg acgcggttga tgccgcgcac ttcgttgatg
     4501 atgcggctgg aaaccttttt gagcagcgca tagggcagct cggcccagtc ggcggtcatg
     4561 aagtcgctgg tctgcactgc gcgcagggcc acgacgtagt cgtaggtgcg gccgtcgccc
     4621 atgacgccca cgcttttcac cggcagaaac accgcaaacg cctggctggt caggtcgtac
     4681 cagctcttgc cggtggcgtc gtcggtgaag ttgcgcagct cttcaatgaa gatggcgtcg
     4741 gcgcggcgca gcaagtcggc gtattccttt ttgacttcgc ccagaatccg aacccccagg
     4801 cccgggcccg ggaaggggtg gcggtacacc atgtgaaaag gcaggcccaa cgccacgccc
     4861 agctcgcgca cttcgtcctt gaacagctcg cgcaggggtt cgagcagctt caggcccagc
     4921 tgctcgggca ggccgcccac gttgtggtgg ctcttgatcg tgatggcctt tttgttcttg
     4981 gagccgccgc tctcgatcac gtccggataa atcgtgccct gggccagcca ggccacatgg
     5041 cgcgaatggt cgttcttgag cttgccggct tcggccttga acacttccac gaattcgcgg
     5101 ccaatgatct tgcgcttggc ttcggggtcg ctgacgccct tgagatggcc gaggaactgg
     5161 tcggcggcat cgacgcgaat gactttcgcg tgcagcttgc cgacaaacat gtccatcacc
     5221 atgtcgccct cgttcaggcg cagcaggccg tggtcgacaa acacacaggt cagctggtcg
     5281 ccgatggcgc ggtgaatcag cgccgccgca acggatgaat cgacgccgcc ggacaggccg
     5341 aggatcactt cctcgctgcc gacctgggcg cggattttct ccaccgcctc gctgatgtag
     5401 tcgcccatga tccagtcggc ccgcgtaccg cagatatcca ggacaaaacg ttcgaggatg
     5461 gccgcaccgc gcttggtgtg cgtgacttca gggtggaact gcacggcgta aaaccggcgc
     5521 tcttcgtcgg ccatgccggc gatcgggcag ctgtcggtgc tggccatcag cttgaagccg
     5581 ggcggcaact cggtgacctt gtcgccgtgg ctcatccata cctccagcat gccgtggcct
     5641 tcggaggtcg cgtagtcctg gatgccttcg agcagctggg tatgaccatg cgcccgtaca
     5701 tcggcagggc cgaattcgcg cttgtgaccg ctttgcacga tgccgccgag ctggtgcgcc
     5761 atggtctgca tgccgtaaca gatgcccagc acgggcacgc ccaggtcgaa caccgcctgt
     5821 ggcgccttgt cggtgctgtc ctcgtagacg ctggcgtggc tgcccgacag gatgatgccc
     5881 ttgagcttgc cgtcctgggc gtagtcgcgg acccagtcgc tggtcacatc gcagggatgg
     5941 acttcgcaga acacatgggc ttcacggatg cgccgtgcaa tcagctgggt gacctgggag
     6001 ccgaaatcga gaatgaggat tttttggtgc tgcatgagag gcgtttcagg caaaaaaaga
     6061 aaaggcgtcc ttcacggacg cctcgggcca tcgggatgat cagtctgccc gatagttcgg
     6121 cgcttccttg gtgatctgca cgtcgtggac atggctttcg cgaatgccgg ccgtcgtgat
     6181 ctcgacaaac tcggcctggt tgcgcatgtc ttcaatggtg gcacaaccgc agtaacccat
     6241 gctggcccgc aggccgccgg ccatctggta aatgatggca accatggagc ccttgtaggg
     6301 aacgcggcct tcaatgcctt cgggcaccag cttgtcggcg ttggggttgc cggtggtggc
     6361 ttcctggaaa tagcggtcgg cactgccctg ctgcatcgcg ccaatgctgc ccatgccgcg
     6421 gtagctcttg tagctgcggc cctgaaacag aatcacttcg ccaggggctt cctcggtgcc
     6481 ggcaaacatg ccgcccatca tgacggtgcc tgcgccggct gcgatggcct tggcgatatc
     6541 gccgctgtaa cggatgccgc cgtcggcaat cagcggcacg ccggtgccgc gcaatgccat
     6601 ggccacactg tcaatcgcca tgatctgcgg cacgcccaca cccgcgacga tgcgggtggt
     6661 gcagatggag ccgggaccaa tgccgacctt gaccgcatcg gcacccgctt ccaccagggc
     6721 cagcgccgcg gcgccggtgg caatgttgcc gccgatcaca tcgacctgcg gatagttctt
     6781 cttgacccag cgcacgcgct cgatcacgcc gtggctgtgg ccgtgggcgg tgtcgaccac
     6841 gatggcgtcc acaccggcct tgaccagggc ttcaacacgc tcttctgtgc cttcgccaac
     6901 acccacggcc gcgcccacgc gcaggcggcc cgaggcgtcg cgtgcggcat tggggaaatt
     6961 ggtctgtttg gtgatgtcct tgaccgtgat caggcccttg agttcaaagt cgtcgttgac
     7021 caccagcagg cgttcaagct tgtatttgtt gagcagggcc ttggcctcga cggcagacgt
     7081 gccttccttg accgtgatca gcttctcgct cggcgtcatg atctggtgcg ccttgacgtc
     7141 gtagcgggtc tcgaagcgca ggtcgcggct ggtcacgatg cccaccacct tgccgccgtc
     7201 gcagaccgga aagcccgaga tgccgagctg ctcggacagt tccagtatct gcagcacggt
     7261 gtgttcgggc gtgatgacca ccgggtcgcg cacgacgccc gactcataac gcttgacctt
     7321 ggccacatgg gcggcctgtt cctgcggcgt cagattttta tgaacaatgc cgattccgcc
     7381 ctcctgcgca atggcaatgg ccagccgggc ttcggtgacc gtgtccatcg cagccgaaac
     7441 caggggcagg ttcaggcgga tgtttcttga gaactgggtg gaaagcgagg tgtccttggg
     7501 caggacctgg gagaacgctg gcaccaacaa tacgtcgtcg aaggtgagcg ctttgccgag
     7561 taggcgcata gggatggctc caaaaacacg attgtacccg cgccggtccg tacaaaccct
     7621 agcgctgaaa ctcctgcctg ccgcgttaca aaaaggccac gcccaacagc gttcatcttc
     7681 gcccgcagga gcgcaaccgg tacactgatc ccatggaact gaaaatcacg cctaaacacc
     7741 ttcttctgct acttgccggc tggacttttg cgctgtcggc cgccgcccaa tggcagtgga
     7801 taggcaagga cgggcgcaag gtgttcagtg accgcccgcc gccaaccgac atccaggaca
     7861 aggacatcct caagcagccc ggcggccggg gacgtgccaa ggcgccagta gccgccaccg
     7921 agccaaccga gggcacccca tccgctgcag cagcgacggc ggcgccggca gcaaaggccg
     7981 cgaagaccaa taccccgaaa atttccggca aggatgcgga gctggaagcc aagaagaaaa
     8041 agctggaaga ggaagaagcg gccaagaaaa aggccgacga tgaaaaagtg gccaaagcca
     8101 gagccgacaa ctgcgaacgc accaaaaagg ccatggtgac catccagtcg ggcgcgcgga
     8161 tatcgaccac caacaccaaa ggcgagcgcg agatcatgga cgacagcggc cgtgcagcgg
     8221 aaagcaagcg cctgcaggca atcatcgaca gcgactgcaa gtaggcagcc gttcgctctc
     8281 agtaccctgc cttggcccct tccctttttt tggcaaacaa ccctgatttt ttcatgccct
     8341 gtttattgaa gcgctcgcgc cgcgcgatct tggggtccac cctcagcggg cgatagatct
     8401 ccagccggtc ctgctggtgc agttcctggc gcaaactggt tttgcgcccc cagatgccca
     8461 ccgacagcct gcctgccgcc agatccggaa actcggccag taggccgctg gctgcaatgg
     8521 cctgcgccac ggtgctgccg gtcgggagtg tcacctgcca ctcgcgcacc tcgcgtggtc
     8581 cgggcgaata cagcacaaaa atgtccagcg ccgtcatggc gctcagacct cggcctcgcc
     8641 atacacctgg gcggcccttt tgacaaaggc atcgaccagg ctgccggcaa tcttgtcaaa
     8701 caccgggccc accagcgctg ccagcgccgc gttgtcgaag tcgtagcgca gggtcaggtc
     8761 gaccttgcag gcgcgctgac tgccgtcgcc caggggggtg aagtcccagt cgccgtcgag
     8821 ctttgaaaaa gggccgtcca ccagcttgag cgagacctgt cggtccggca catgggtgtt
     8881 gcgggtggtg aaactctgat gaatgccggc aaaggcgatg cccaccttgg ccgtcatgcc
     8941 ctgcgcgtct tcttcgacca cactggcctg atcgcaccag ggcaaaaact gcgggtagct
     9001 ggcgacatcg gtgaccaggg caaacatttc ggctgcgctg taccagatca aaacggactt
     9061 gtgaacggtt ttcatacaat catgggctgc gctcgattgt attagccgat gccgccctta
     9121 ccttttaccc atggccacca aagaagcatc ctccaaaaaa ccggccgccg cgtcatccgg
     9181 aaaatccaca ggcaagcccg ccatcaacct gacacccaag gctgccgctg ctgccgtgcg
     9241 cattgccgac aacaagaaag ccacgttcaa ctaccacatt gaagagcgct tcgaggccgg
     9301 catggtgctc gagggctggg aggtgaagtc gctgcgcgaa ggcaaggtgc agctgaccga
     9361 cggttatgtg gtgatccgca acggcgagct gttcctgatc ggctgccaga tcaatccact
     9421 cggaacggca tccacccatg tccggccgga ctcggtgcgc accaaaaaac tgctgatgca
     9481 caaggacgag atccggcgcc tgatcggcaa gatcgagcaa aagggtttca cgctggtgcc
     9541 cctgaacctg cactggaagg ccggcaaggt caaatgtgag gtaggcctgg ccaaaggcaa
     9601 gggtgaacac gacaagcgcg acaccatcaa ggaccgcgaa ggcaagcgcg aggtcgaacg
     9661 cgcgatgaag tcgcgcagcc gctgacttcc gtgcgaggct gaaccctgtc ttgcagaaat
     9721 atttgacgag acagggaata aactggctcc aataggtgtt tgtgtggatg cagagctgct
     9781 ctgcctcacc cccctcacct accggagttc accatgaaat acctgaccat cgccgtcctc
     9841 accgccgccc ttctcggcgg ctgcgcttcc atgtccagcc atcccgccaa agtcgccgac
     9901 ggcgtgctgg tcggcccgaa cggcatgacg ctgtacacgt ttgaccgcga tgtgacgggc
     9961 agtggcaagt ccgtctgcaa tggcccttgc gccaccaact ggccgccgct gatggccgcc
    10021 gcgacggaca aggcctcggg cgactacacg gtcatcacgc gcgacgacgg caaaaagcag
    10081 tgggccatga aaggcaagcc gctctactac tgggtcaagg acaccaaggc cggcgacagg
    10141 accggtgacg gcgtgcagaa cgtctggcac atcgtcaagc cctgaacctt cttcagtcgc
    10201 acgactgcag cccatggacc gttccgagct gattgcacag attcccgggt tgcggcgtta
    10261 tgcccgcgcg ctgaccggca atgcctgggc cgccgacgac ctggtgcagg acacgctgga
    10321 gcgggcctgc agcaaatggc gtctgtggac ggccgggtcc gatctgcggg cctggttgtt
    10381 caccctgatg cacaacctgt tcatgaacca gcggcgcagt gcggcgcggc aatggcaggc
    10441 tggccccgcg ctcgacgtgg atgagctggc ccacacgctg gccgcacccg acgccaggca
    10501 ggatcaggcc ctggacctgc agcgttgcct gctgcggctg ccggacgacc agcgcgccgt
    10561 gctgctgctc gtcagcttgg aagacatgag ttacgagcag gtcgcgcgcg tggtcggcgt
    10621 cccgctgggc accgtgatgt cgcgcctgtc gcgggctcgc agccgcctgc gtgacctgat
    10681 ggacaagcct gcgcaagctc aggcggcaac cgacagcgcc cgaaacgttt ccgccttgcg
    10741 gcgcctcccc tgacaagccc acaccgccat ggatgcttca agaacatcgc ccctgaccca
    10801 cgacgaactg aatgcgctgg tcgatgggca actgacgccc gggcaacggg ccgccgccat
    10861 ggcgcaggtg gccgacgacc cgctggctgg cgaaaccttg acagcctggc aggatcagcg
    10921 cgaagcgctg cgcggcctgc accagaagct gctggacgac cccattcccg aaaccctgct
    10981 ggcggcggtg caacgcagca cgcgcgagcg gcagacgctg gacccgtggt ggcgctgggg
    11041 cggcatggcc gccggcgtgc tgctggcatt tgccgtcggc tggacggcgc acggtcagtt
    11101 cggcaacagg ggttcttatg ccgcaggcca ggcagcacgc gccagccagg cgtttgcacg
    11161 ccaggccacg ctggcccatg cggcgtttgc gccggaagta cggcatccgg tggaagtcgg
    11221 cgcggcgcag caggagcacc tggtgcaatg gctgtccaaa cgcctgggca agccgctcaa
    11281 ggtgcccgtg ttgtccagcc agggatatga actggtcggc ggccggctgc tgcccggtga
    11341 tggcggcgcc cgggcgcagt tcatgttcca gaacgctgcg ggcgtgcgcg tgaccctgta
    11401 cctgggggcg ctcgatggca gtcccgtggc aggcaagccg gtggacgcgg aaaccgcttt
    11461 tcgttacagc aacgacggcc cagtgccagc gttttactgg atggaccagg gcttcggtta
    11521 tgccatgagc ggcccggtcg cgcgcgagga gctgatgcgg ctggccgagc tggtctacca
    11581 gcagctttag aacggtttta ttgaaggtcg cgcaatggat gcgcgtcggc cgcccaggga
    11641 ctggcgaaaa acgcctgcac catctgcggc ttgacgtctt caatgcgggc cgggttccac
    11701 ttcggcgtgt agtccttgtc gacggccagc gcacgtatgc cctccaccgt ctcgctggcc
    11761 gcgcctggcc tgaggtgaaa gcaatggtgc accatgtcgc gctccatgcg caaatcgtcc
    11821 gccaggttca tgcttctggc gcgacggatc tgctccaggg tcacatgcag catcaacggt
    11881 gaacgtttgc gcagcacgac ggcggtcttt tgcgcccatt catcgtcctt c
//
LOCUS       DAIDFG010000062        11925 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_62_length_11925_cov_16.981803, whole genome shotgun sequence.
ACCESSION   DAIDFG010000062 DAIDFG010000000
VERSION     DAIDFG010000062.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 11925)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11925)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11925
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62_length_11925_cov_16.981803"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2218 a         3968 c         3676 g         2063 t
ORIGIN      
        1 gtgacctggt agccactgac cctgagccag cgctggatca cgtcgaaggc catcatcatg
       61 cgggcatggc cgatgtggca caggtcgtaa atggtcatgc cgcagacata catgcgcacc
      121 tggccgggaa ccaggggcga aaaatcttct acggcacgcg tcaacgtgtt gtaaatgcga
      181 aggctcatga aaagtctggg tgttcagtct ggctggaatc gccggaaccg gaagggggcg
      241 ctggagcggg ggcgggtcat caggtgcatc agcaaggggg aaaagctgct ccgaaaggtc
      301 tggcaacggc atgccaaggg tgatttttcg caggagctta aactataccc ctcagataca
      361 atcatccagt atagccagcc aggaagagcc catgcagcac gctgcacagc cggcttcttc
      421 gtttcctgtt tgataccagc cactccatgc gttccagagg aaatatgtcc aagtcagctt
      481 atttttcaaa ccaggcgctg ccgactgccc tgcgcttgct ggctctggtc gcggccctgt
      541 gtgtgccgcc cgctcatgcc gacgattaca ccgaggtgaa cgccctcgtg cgcgccgggc
      601 agctggggga agccatggcc agggccgaca aatacctggc caccaagccg cgcgacccac
      661 agatgcgttt catcaagggc gtgatccaga ccgaagccgg caaaccgggt gacgcgatca
      721 ccaccttcac caagatcaca caggactacc cggagctgcc cgagccttac aacaacctgg
      781 ccgtgcttta cgccggccag aaccagttcg acaaggccag ggccgccctc gaaatggcca
      841 tacgcaccaa cccgagctac gccaccgcgc atgaaaacct gggcgacgtg tatgccaagc
      901 tggccagcca ggcgtacagc aaggcgttgc agctcgacgg cggcaacagt gccgtgcccc
      961 ccaagctggc actgatccgc accctgttca cgccggaagc caaaacccag aagccggccg
     1021 cagcgacacc aaccgcgccc gccgtgacgg cccagacacc tgccgccccg gccaaaccgg
     1081 cgccagcacc tgctgcaccg ccaacaccgg ccgcagcccc cgcccctgcc gcgcccgccg
     1141 caccggtatc ggcgcccgca ccggccgccg ccacaccggt cagcgccgcc gaaaaggaag
     1201 ttgaaacagc cgtcagggcc tgggccagcg cctgggccgg caaggacatg accgcttacc
     1261 tggccgccta cgccaaggac tttgacacac cgggcaacca gtcgcgcaag gcctgggaag
     1321 aagagcggcg caaccgcatc gtcggcaagt caaagatcag cgtgaagatc aacaacctgg
     1381 cggtcgacgt caagggcagc aaggccaccg ccaaattcag gcaggagtac agcgccgatg
     1441 cgctcaacat ctccagccgc aagacgctgg acatgaccaa ggccggcgaa cgctggctca
     1501 tcgtcaggga atccaccggc ggctgatggc agggacctgc gtgcgcaccc ctggcacttg
     1561 cctgacattt ttcacggcac ctgacccctc acccatgcaa cgggcatccg ccacgctcct
     1621 cgtcacggca ctggcggtga tgtgcggctc cgccctggcc cagagcgtgg acaagagcaa
     1681 gccccccccc tccaccacca cttcccccgg cagcgcagca gccgacaaac gccgtgcagc
     1741 gccctcccgc gcagccgtgc ggccggcggt caaggacggc gaagccgaag cccgcctgat
     1801 cgagatctac aaactcgccg gccaggccag gggacgcgag gcactggtca aggccgagcg
     1861 cctggtccat gaccacccca acttccagct cgcccagctc gtttatggcg acctgctggc
     1921 cgcccggtcc cgtccgctgc gcgcgctggg cgatgtgtct gagggaacgg ccaaggccgg
     1981 tgccagcacg ctggccgagt tgcgccagga atccctgatg cgactcaagg cgctgcgcga
     2041 gcggccggcg ccgggcacca ttccctcgca gttcctggca ctgtcgccgc gcaacaaaca
     2101 tgcgatcgcg gtcgacgcct cgcgggcccg gctctacctg tttgaaaaca acgacaccgg
     2161 tctcaagctg gtgggcgact actacatctc ggtggggaaa tccggcatcg aaaaatctgt
     2221 cgagggtgac tcgcgcaccc cgctggggat ttatttcgtc accagcaacc tggacccgaa
     2281 gtcgctgaaa gacttttacg gctcgggcgc cctgccgatc aactacccca acgcgctgga
     2341 cctcaagcgc ggcaagaccg gcagcggcat ctggctgcac ggcacgccgc cgacccagtt
     2401 ttcgcgcgcg cccctggcca gcgacggctg cgtggtgctg gccaatccgg acctggagcg
     2461 catcatccgt accgtggaag tgcgcaccac gccggtggtg attgcacaaa gcctcacatg
     2521 ggttgcgccg cacagcatca aggccgacag caagtcgttt gaagaggcac tgaacagctg
     2581 gcaccaggcc aaatcagtcg gtgatctcgc caccctgacc agcctgtaca gcagcgactt
     2641 cagcagctac ggcaagacac gcgatgactg gacggcccag ttgcgttccg aggccgacaa
     2701 acggcgcggc cgcaccgtgc aactcaagga cgtgtcctac ctgcgctgga ccgacagcac
     2761 cgataccatg gtggtaacct ttggcgaggt gctcagcggc tcacgcaccg gtccggtccg
     2821 gcgccagtac tggatgcgcc agggcaagga ctggaaaata ttctttgaag gagtgattgg
     2881 atgactttca gcaacaaggc catcggccgg cgtgcctgcc tggcgatcgc aacgctggcc
     2941 ctgctggccg gtcaggcctg ggcgcagggc gcggccagcg cgcccagggt caggtttgcg
     3001 accagcgccg gcgatttcgt cgtcgaggtc tatcccgaca aggcgccaaa aacggtcgcc
     3061 aattttcttc agtacgtcag ggacaagcac tacgacggca cggtgtttca ccgcgtgatc
     3121 gacaacttca tggtccaggg cggcggcttc gatgccagct acgcgcaaaa acccacacgg
     3181 ccgccggtgg tgcatgaagg gcgtgaagcg ctggccaaag gcggcgacaa aaacgtcgtc
     3241 ggcacgctgg ccatggcgcg caccaacgat ccgaactccg cctcgtcgca gtttttcatc
     3301 aacgtcaggg acaacgactt cctgaacccg agcgcccagt ctccgggcta caccgtgttc
     3361 ggcaaggtgg tcagcggcat ggacgtggtc ggcaagatca aggccacgcc caccggcccg
     3421 ggcggcccct tcccgagcga cgtccccaga acacccatcc tcatcaactc tgcaaccctg
     3481 gtcaaataaa tccccatgag caacccacaa gtcgaactcc acattgccgg ccacggcgtc
     3541 atcacccttg aactcgacca ggacaaggca ccgaaatcgg ttgccaactt cctcagctac
     3601 gtcaacaagg gccactacaa caacacggtg ttccaccgcg tgatcccggg cttcatggtc
     3661 cagggcggcg gttttgaagt cggcatgaaa caaaagccca ccgacggcga gatcgaaaac
     3721 gaagccaaca acggcctgaa aaacgaccac tacaccgtgg ccatggcgcg caccaatgca
     3781 ccgcactcgg ccagctcgca gtttttcatc aacgtgtccg acaacgcttt cctgaaccac
     3841 accgcgccca acgcttcggg ctggggttat gcggtgttcg gcaaggtggt gggcggcacc
     3901 gacgtggtcg acaagatcgc cgccgtcaag accggccgca agggctttca cgacgacgtg
     3961 ccacaaaccg acgtggtgat tgaaaaagcc gtcctgctgg cctgatctcc gtctggactg
     4021 ggcccgttca agttcagccc gtgaaaatcc cgtcgaccgt tccccgggtt gcggagctcc
     4081 atgcgccgcc ttcctggggc acggtcgatt tcatttccga cctgcacctg caggcggccg
     4141 atgccgccac gtttgccgcc tggcgcgact acatggaaag caccaccgcc gacgcggtgt
     4201 tcattctggg cgacctgttt gaagtctggg tcggtgacga cgccgtcgat gtgacggcag
     4261 gcgctcaaga caacttcgaa gaccgctgcg cccgcctgct cggccagacg gccggccggc
     4321 tggcgctgtt cttcatgcac ggcaaccgcg acttcctggt cggcccgcac ctcatggcgt
     4381 tgtgcaacac caccctgctg gccgacccga ccgtgctggg cttcaataac cagcgctggc
     4441 tgctctcgca cggcgacgcc ctgtgcctgg gcgatgtgga ctacctgcaa tttcgccgcg
     4501 aggtgcgcag ccccgcctgg caacgcagct ttctggcccg gccgctggcc gagcggcagg
     4561 ccattgcccg cggcctgcgc gagcaaagcg aggcgcgcaa gcagtccggc gccccttatg
     4621 ccgacgtgga cagccgcgcg gcccgcgagc aactcaaagc cgccaacgcc cagatcctga
     4681 tccacggcca cacccaccgg ccggcggtgc acgacctcgg tcacggctac tcacgcgtgg
     4741 tgctcagcga ctgggacgcc gcagcagccc tgccgcgcgc ggaagtgctg cgcctgacgg
     4801 ccaatggcct gcgccgcatc ccgttgttgt aggcggtcgc catgctgaac tggttgcgcc
     4861 agctgttcct acgcacgcca cgcgccgaca ccattcccga cgccctgtgg cgggccacgc
     4921 tggctgccca ttcttttctg gccgagcggc cggcggcgga gcggctgcaa ctgcgccgcc
     4981 tggtcggcga gtttctggca cacaaggaat ttaccggtgc gcacggcctg gtggtcaccg
     5041 acgagatggc ggtggcgatt gccgcgcagg cctgcctgcc ggtgctgcac ctgggactgg
     5101 gctggtacag cgacttcgtc ggcattgtgg ttcaccccgg gcagatgatc gcgcgccgcg
     5161 aaatgcagga cgccagcggc gtcgtgcacc gctacagcga ggtgctgtcc ggtgaagcca
     5221 tggagcgcgg cccggtcacg ctgagctggc aggacgtggc ctcggccggc gcgctggccg
     5281 gccacggtta caacgtcgtg atccacgagt tcgtccacaa gatcgacatg aaggatggcg
     5341 ccgccgatgg ctgcccgccc ctgccctcgc gtgccgcgca caaggcctgg catgacgtga
     5401 tgcagccggc ctacgacgcc tttcgtgaac aggtcacgat ggccgaacgt ttcggcggcg
     5461 agccaccctg gctggacgcc tatggcgcca cggcaccggc ggaatttttt gcggtgagct
     5521 gtgaagccta cttcgtgaac cgcgagcgct tcacgcagga ctttgcggcc ctgaccgcgc
     5581 tgttcgacca gttcttcaag gtcaatcggc ctgcagccct tgaaggacgg gcgcagtaag
     5641 ctatttaatt gatagcgatt gacgactgtt ccacgcggcg cgccggggtg gccccgggcg
     5701 acaccgcaag gatcccgcct ctcgtcgctt gatcggcgct gatcagcgct tgatcagcgc
     5761 cttcagcaca ttctgcagac gccgcacatg cgccgcgtca tacggcgcgg ccagcacttt
     5821 ggctgcgtcc tcagcccagg tctgcgccgg cgccgggtcg ttgcgtttgg tcagcagcac
     5881 cagctgcaac atgcggcgcg cgctgatgtg ttcggccggg gtcggcagct cggcggcaat
     5941 ttccaggcgc agcagggttt ctgcggcatc ccccttgggc gccgacgaca gggccttcat
     6001 ccacgcgctg cgtgtggccg gcgtcaccag cttgcccaag gcctgggcat ccggcagcag
     6061 cgcagggtcg cgcttttccc aggccgtcag caggtgggtc aacacttcgc cgtgcgcctg
     6121 ttcggagagt ttgcgcagcg cctgctgcgc gtgttcgaaa gcttcgcgct gcgcgcggaa
     6181 ggcggcgtcg cccagccgcg ggccacgttc ttcgtaggcc ggcctgtcgc tgaaacggct
     6241 ttcccgctcg gggcggtagg gttcgaactt gttgtcggct ggccggttgc cgcggccgtc
     6301 gggcttgccg ccgccgggtc gcgcgtcctt gcggtcgcca aacttgccgc cgcggcctgc
     6361 gggcgcggct tcggccttgc gcatgccagg gcggtcatcg ccgcgcatgg ccaccaccgg
     6421 tttggcgggt ttgggcggcg gcgcggccgg tgccggcgca gcttcttctg cttccgcagc
     6481 gggctcggcc gccgcttcag cttcagcctc ggccggggcg tcctctttcg ctattgtttc
     6541 aggagcggct ggcgcagacg catcgggcgc tgacgcctga tcgggctcag aaccctgcgg
     6601 tgcttcggtc gctgcaggtg caggcgctgc gacggcagcc ggtgcggccg gcttgaactg
     6661 cccgcgcaaa ccagcttcca gatcggccat cgcggtgcgg attttctgca cgtcgccggt
     6721 ggcgttggca gcgtccaggg ctttggacaa ctccagcacc aggcggtcat gctcgcccaa
     6781 ggccgacgcg gctttttcac gctcggcggt cttgcgctgg aaggcgtcgt cgatcggctt
     6841 gcggaaagcg tcccagagtt tttgctcctg cttgcgctcg atcggcaccg cctgcgcttc
     6901 gtgttgccag cgctgctgca gcgccttgac cgcgtcgatg tgcagttgcg gctcggcgcc
     6961 cagcacctgc gcttcctcga tcatggcctt gcggtgcgcc aggctctggg cgcgtgcggc
     7021 tttcagcggc gcatcggcgg catccatcgc ggccttccac tgcggctgga tctcggcaaa
     7081 ggctttttcg cccaggtggc cggcggccgt ccacttctcg acaaaaccat tgaggctgcg
     7141 gatctggaac ttccagtcag tgttgccctt gttggcctcg gcccaggcca acacctcgtc
     7201 gatcagcgcg cggcgctcgg ccttgacggc ttcgctttgc tccttgacct tctcgagcca
     7261 gacctcgacc accttgtggg cttcgttgca ggcgtcgtca aaacgcttcc acagcgcgtg
     7321 gttggggatg ccgccctggt cgctggtttt ccagagttcg cgcatggcgc gcagcgcctc
     7381 ctgctgcttg cgcccgccca ggggcttggc cactagcgct tcggccttgg ccaccagttc
     7441 ttcacggatc tggtcggcac gccagcgctg ccagccttcg agttcgccgg ccgccgtcaa
     7501 tgcggcatgt gccgcggctt cgagtgcgct gtcgatgttg cggatgtttt ccttcagcgc
     7561 ctggcgcagc tcggccgcga ccttgggcgt ggatttgcca tggccttcgg tgacttcatg
     7621 ctcgagcttg gccagcgcag cctgcacgat ggccgccgat ttttcacgca tgtccttgag
     7681 cacggcggga tcgatcttga ccttgggcgc gcgcgcgggc tgctcaccgg cagcggcgtc
     7741 gccgccacgc ggcgtgccgc gtacgctacg cagctcatcg gcccagaccg gcaccgccgg
     7801 cagcggtgcc gcggcgtctt cggcggccgc gacggccagc gacagcgcgc cctgaaaggc
     7861 ttcccacacc gccagcagct ggccgcgcga ggcgtcgagc agcggcggga acttggcgtc
     7921 cacgctgggc cagttcgcgt cctgcgcgag ttcgtcggct tgggcctgcc agtgcgtcac
     7981 atcagaacgc agggcttccc acacgccttg tgcatcgcgc cagggtttgg tcgacagcac
     8041 ttcaatgcgc tgggcaatca gcacggccgc ctcgcgctgc acctgggcgc ggtgctgcag
     8101 gtcttcaatc gtcttgacgc gctcggccag ctgggctttc agcccggcca gcggctcgcg
     8161 cgacagcgga gcaccggcct tggcggcatc acgctgccag gccagcgcat cggccatgtt
     8221 gagctttttc agggccagca gggcatggcc tttttcagcc cactcggcgg ccacggtctc
     8281 ctggcttttc aggcgcttgg cttcgtcgag cttttccttg agcggcttgg ccgcaccctt
     8341 gtcccggttg gccaggtcct tgtagacctc ggccatctgc tcggccgtgg gtccggtggc
     8401 gagccactcg cgaatgcgcg cggtgcgttc acccgacgtg ggcgctgaga aagccccgcc
     8461 cgtcaggcca tcgagcggat ggctgtcggc gctcttggtg gaaggcgttt tcacggcggg
     8521 tgtttcgggc ttggcagatt tggacatggg gaaacgcagc atatggataa gtaggacggg
     8581 cgaagcgggg atcttcaggg atcaggacat tgcaggtctg ctccttgggc gctgtcaccg
     8641 gttggcgtga acaggcacgg ggggcgggac cggcgtaaaa aagaggagcc gcaggacaga
     8701 tatccgcgac cccgacccaa gcttcagaaa taaagcaatg cggctatttt cgctgttttt
     8761 agggcatcgc caaattaagt tcggcacggg gtgtccattt ggcgtctgaa ggaacacttt
     8821 tgggtgcacc caaaagagcc tgccgggcgc aataccactt gtaccgaggt agaccctgag
     8881 cgccacggcg cgctgcacgg ccagttcgcg catggcgttg tcgcccactg ccatgctggc
     8941 cgtcgctgag cacgaggttg cagagggcaa aacgcagcgg actgctggcc ggttcatcgg
     9001 ccggaggctt caggcgtgcg aggatgtcgt caacgtcata acggccctga cccagatggg
     9061 tgagcgacaa cgtcaggcca tcgacctcga tggcgccggc aaccggtgca aggcgcagga
     9121 gcgactgcat ttctgcatct atatagagac gtgcaatttt cagctggggt accccttgga
     9181 aaagcaggct gctttccggc cccgcagcag gcgccggcgg gctgccggct tttgcgatgc
     9241 gatggtcaga tcacgggggc cagctccagc aaccacggac gaaagtccac tgcgcccacc
     9301 gtgagctgcc ggcccagctt ctgagaaacc gcttttcaag ttgcgacttc agcaccaccg
     9361 gcaccaggat atagaccagc gcccagagcc atagcaagcc acccacggcc cgcgctgcgc
     9421 ggcgatgcca gagcggtgaa cgcagcacgg ccactgcttt gccgggagga gactggagag
     9481 tcgggaggga tcgcgtttca gttcacggtc gggcataggc cttgagtctt gaacgctcgg
     9541 ggaaaaggtc aatggctgat tgggctaatt tggccaaaaa ggccggcctc aaaataaaaa
     9601 ccccggacag caaaggccga ccggggttga cggcgggcgt gatgctcatg ccgtcgggct
     9661 tcgcaaagaa gagaagatcg tctccagtgc gatgaaagca agtgattgct ctcagtttga
     9721 ccgggattgg ttacccggct gatgcttgag gtactcaagc aattgcagat taaacctccg
     9781 gcacccgagg cgcaagtcga aatttgtaat caaaaatcag cccgcgattg ccattttctt
     9841 cggagttaca tctttgcgcc cttctcctac atcgccatga ggtattcgcc gcatcgcctc
     9901 caaaaaacgt cctgcttgaa tgctccggca atttctctga aaaacgctgc aagcacttga
     9961 gttaaaagcc cttactggca aaacatacag cttttctctc aaaataccgc gccaacttca
    10021 cgacctagag cgaaacccca aaaaaccgct caaatgggag aaatgggggg tcttgacccc
    10081 ttatttcagc ttttaatgca ttattttgca tatgaaatag cttttatatt attgacaagt
    10141 tatttagcgc cctcataaaa tccgccgtcc catccagaac tacgggacaa cccgaatccg
    10201 ctgccgaacc cggcaaagtc agacaagggg tcaacacccc caaggccagg ccttggtttg
    10261 atggcgaccc aatcaaagcg tacggctcaa gaagtcgatg cgcatagaaa ggagtgctat
    10321 gacagcgcca ggacaatcca cccccgcatc ggtaatgcgg acacttcaga aaaacgtgag
    10381 caacagtctg gcaaacattg ccgacgacat tgcccagggc ttttttgaaa ttacccacaa
    10441 cagcttcgca ctcgtcggtc tggccgtcgt gttcgccgtg atcacgctga ccgcccgtcc
    10501 cgacctgcgt caggccggcg aatcccggct gatgacctgg ctgcaggagc gccaggaagc
    10561 cgcgattggc ttcaccgaac ccgatgccat cgaccgggcc accgcagcca accccaaaga
    10621 cctgccccgg caacaggcgg cggtagcctt ctggctcagc aaaaaataca acgtggcgcc
    10681 cgaacccctg agtgtgctgg ttgccgaagc cttcgagatt ggccagaagg ccaagctcga
    10741 ccccacgctg atcctggcca tcatggccat tgagtcgggc ttcaacccgt ttgcgcaaag
    10801 cccggtgggt gcccagggcc tgatgcaggt catgaccaac atccaccacg ccaagtacga
    10861 aaacttcggc ggcaaactcg ccgccttcga cccggtcacc aacctgcggg taggtgtcaa
    10921 ggtgctgcag gaatgcattg cccgcgccgg ttcggtcgaa gcgggcctga ggttctacgt
    10981 gggcgcagcc aacctggaaa gcgacggcgg ttatgccgcc aaggtgatgg ccgaacgcgc
    11041 acgcctgctg gctgttgtgg gcggccgcgc accggcctac atgccgacgg tgcgcccgcc
    11101 gctggccatc ccagcgtccc tgcccgcaac cacagacgcg gaagacctgc ccggcggcaa
    11161 ggccggcgag aaagtcgcca gcctcgccag ctcctgagcg cggcccggcc tttcacaggc
    11221 cggtcgtcag ccagacgccg ggtccgttac actaggggtg tgcgcgactg gcgataggca
    11281 gcccctgttt tgcagggcgg ttgctgacgt gggataccac tgggaagcgc accgctgggc
    11341 tcaagccatg accacagcgt tcagccgttc gcctgggcag cccttgtttc attcatccga
    11401 tgacagggac cacgtcttga aggactgcca tgtacgaccg caccattctt gtggaacaaa
    11461 ccgaccccga actgtgggct gccatcgttg ctgaaaacgc gcgccaggaa caccacatcg
    11521 agctgatcgc cagcgaaaac tatgcctcgc ccgccgtcat ggccgcgcag ggttcgcaac
    11581 tgaccaacaa atacgctgaa ggctaccccg gccgccgcta ctacggtggc tgcgagcatg
    11641 tagacgtggc agaacagctg gccatcgacc gcatcaagaa aatcttcggt gccgaagccg
    11701 ccaacgtgca accgcattgc ggcgcctctg ccaacgaggc tgtcttcctg gcctttctca
    11761 agcccggcga caccatcatg ggcatgagcc tggccgaagg cggccacctc acccacggca
    11821 tggcactcaa catgtcgggc aagtggttca acgtcgtcgc ctacggcttg aacgaaaaag
    11881 aagaaatcga ctacgacaag atggaagcgc tggcacgcga gcaca
//
LOCUS       DAIDFG010000063        11833 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_63_length_11833_cov_18.251995, whole genome shotgun sequence.
ACCESSION   DAIDFG010000063 DAIDFG010000000
VERSION     DAIDFG010000063.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 11833)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11833)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11833
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63_length_11833_cov_18.251995"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2206 a         3740 c         3778 g         2109 t
ORIGIN      
        1 gccgctgcgc cttgcggccg gcggagccgg accgcggccc ctgcttgtct ggcgagtcag
       61 ttaatttatg aaccaactcc aactttttct cccgtgcgcc gccggcgtcg aagaacttct
      121 tgccgccgag gtcaaacgca tcaccggcag caccggcaag gcctggcgcg ccggcgtgca
      181 ggtgacgggc tcttggcgcg atgcgctgca gctcaacctg cacagccggc tggcgcagcg
      241 cgtgctgatc gagctgcagc acaacagcta ccgcagcgag caggacctgt acaacgccgc
      301 agccggcgtg gcctgggaga tctggttcac gcccaaacag accttcaagg tcgagatcac
      361 cgcacagcac agcccgctga ccagcctgaa ctttgccgcg ctgaaaatca aggacgccat
      421 cgccgaccgc ttccgcgaca aatacgatgt gcgccccgac gttgacacac gctggcccga
      481 tgtgcgcgtt tacgcccacc tgaccaccga caccgtcacg ctctacatcg acacctcggg
      541 cgagccgctg ttcaaacgcg gctggcgcga ggacaagggc gatgcgccgc tcaaggaaac
      601 cctggcggcc gcgatgctgg ccgccagcgg ctggagcggc gaggcaggcc tgtgcaagca
      661 gggtgtgccg ctgtacgacc cctgctgcgg ctccggcacc atcgtcatcg aggcggcgca
      721 gatcgcctgc aacatcgccg ccggcatggg ccggcacttt gcgtttgaaa aattcgtgcc
      781 cttccagccc cacgtctggg aaggcctgct ggaccaggcc gagcaggcca tcacgccgcc
      841 gacggcgccg gtgttcggca gcgacgtgtc cttccgcatg gtcgacttcg ccgaacgcaa
      901 cgccgaacgc gccggcgtct cgcacgcggt gcagctgcgc ggcggcgacg cgctgcagcg
      961 catgccgccc gcgccctcgg gcgtgatgct ggtcaatccg ccctacggcg agcgtattga
     1021 cgtggccggt gtggccggca ttcccgcgct gcagggacgc gaccagcgcc gccagcaggg
     1081 cggccaggcg caacaggacc accgcaacca gcccatcgac gagttcggcc agcccttgag
     1141 ccgtgacgcc ggcgacagcc gcgacacccg ggacacgcgc gaagagcccg ccttcgaaac
     1201 ctcgcgcgac cgttcggcca acccgggccg cgagcaggcc gtcaatgcct ggggcgaaga
     1261 ggcctcggac ttctttccgc aactggccag ccactggaag aaaaactacg ccggctggac
     1321 cgcccatgtg ctcacgcccg acatgaagct gccgcaaaaa atgcggctca aggagtcgcg
     1381 ccgcgtgccg atgtggaacg gccccatcga atgccggctg ttccgcttcg acatggtggc
     1441 cggctcggcg cgcgagaaaa agcccgccgc cacaccttga gcccggccgc catggacacc
     1501 caggcgcggg ccggcgactg cgtggtgctg gacaccaaca tcgtgctgga cctgtttgtg
     1561 ttcggcgacc tggcggcgca ggcgctcaaa agcgccgtga tcaccgggca gctgcaatgg
     1621 ctggccaccg aggccatgcg taccgaactg gcgcgcgtgc ttgactaccc gcaggtcgcc
     1681 aaacgcatga cgcgcgacgg cctggttgct gcgcaagtgc tgctggcctt cgacacccat
     1741 gcgcgcaccg tcgcagccgg ccccaaggcg ccggtgacct gcagcgacgc cgacgaccag
     1801 atcttcatcg acctggccgt ggcgcacaaa gcctggctgc tgagcaagga tcaggccgtg
     1861 ctttgcatga aaaaaaggct gctggccctt gatgtacgtg cgcaatcagc tattgatttg
     1921 atagctgttt gaggttttcc gccggagaac gtacgccgga agaacggctt cagcccagcg
     1981 cctgcacgac cgcagcgaag aagctcgcag gctgaatcgg tatggttcct ttgggtttgg
     2041 gcatggcgct ccactgggcg atgaccacct gttttgcggg gtggatgtac aggtactggc
     2101 caaagatgcc gatggcaaga taagcgccct ggtgcaccgc gccggccgag gcggccaggg
     2161 gccaccacat atagccgtag ggcgtctggc tgtcatggcc gctgttgctg caggcctcct
     2221 tgatccagtc ctggggcagg atctgcgtac caccggccac gccgccgccc agcatgaaca
     2281 ggccaaaacg cgcgaagtcg cgcaggcgtg cgctcaggcc gctgcccgcc acctccattc
     2341 cgcccggcga ctccagccac cagctggcat cggcctccat gccgaagcgg gcccagatgc
     2401 gttcggacaa atagtcggac agcggctgcc cgaccgccgc gcgcagtacg gcgccggcca
     2461 cctgggtttc gccggtgctg taattccata ccgagcccgg cgggccggcg cgcggcaacg
     2521 cggccatgca gtccatgagg gcgcccgggc gctggctcag ttgcgcttcc agcaggcggc
     2581 gccggtcgga atgggggtcg gtgtaggttt cattccagcg cacgccggag ctcatctgca
     2641 ggacctgccg gatcgtcacg ccctcatagg ccgagcctgc aagcgtcggg acgtagcggc
     2701 agacctgctc gtccaggctg ccgatgtggc cgtcgtggat ggccgcaccg accagggtgg
     2761 cggtgatgga cttggccatg gacatcgagg tccagcgcgt ctgcggtgta ttgcccagcg
     2821 cgtaatgctc gaaggcgacc tcaccgttct ggagaaccag caagccgctg acacggttca
     2881 gggccagata gtcgaaaaga tcccagcggt ggcccccatc gtcgaacccg atcgcgggcc
     2941 agggccggga actcacgggc aaggcatgaa cgttggcacc gcgcgccacg acgcgggtgg
     3001 ggaacaggtg gtccacatgg cgaagcgtgc tgacctgcag gtcgggcagc agggcgccat
     3061 cgtaaagatc gcggacggag ccgatggcct ccaggccgga agctgcgccg gccgcggctg
     3121 gctggtcagg catcgggcgt tgcatcgaag ccgctggcag gtcggaatgg acgatggagg
     3181 cagccggagg cgtcatgccg ggacgcccag acccagcgca agggcggtgt agcggatggg
     3241 atacaccgcg ttgatacggt caagcatgca ggcctctgta aaaggagctg ggtggtgcga
     3301 aagcgccggg ccggcccgcg tggggcctgc ggctacactg cccatcatac gagttaaacc
     3361 accacggaca ccccatgaca gacgccgccg cccctgccga ttcgaccttc ccgggcgccc
     3421 tgagccccga ggatttcgaa acactggaca ccatcctcga cgacctgcgc aagcgcgagg
     3481 aagaaacgcc gcagtgggag ttctgcgagg gtttcctggc ggcgctcgcc tgcacgcggc
     3541 gcgagattcc gcccgacgaa tacatggccg tgctgctcga cttcgagggt gccgaagacg
     3601 aggccgctgc ccggcagctg tttgccagtg acgagcagcg cgcgcagttc atggacctgt
     3661 gggagcgccg ctacaaagac atctgccgcg cgctggatgc cgacgtggaa aacctgcagg
     3721 acgagcgcgc cttttacccg caggtcggcg acatgcgcgg catgctggcc cggctgcccg
     3781 aggaagagcg caaggacatc gacatggaca gcgtgccctc gttcgcccag gtctgggcgc
     3841 tgggtttcat gtacgcggtc gagtcctggc ccgacgagtg ggcgctctca cgacaggaag
     3901 ccaaggacaa gcaggccgtc gaggacctcg acgccgcgct gggcgccatc atcgagctga
     3961 ccgacgacga cccgggcccg atggagatgc ccatgctcgg cgaagacgcc gcgcccagtg
     4021 ccagcgaagt gcgcctcgaa caattcggcg aagccttatg ggcggtgtac gacctgcgcg
     4081 gcttctggcg cagcttcggc gccaaggtgg cgaccattca caaagaggcc acgcccggcc
     4141 gcaatgattt gtgcccctgc ggcagcggca agaaatacaa gaagtgccac ggggcggggt
     4201 gagcccgggt ctctggtttg accagagagc gtgaaatcag cgcggtgcgg gccgctgccg
     4261 catcaagcgg gacttcgccg agaacaggag cgcacgggaa tacagcttgt gaaagccaag
     4321 cagcaggcga aaggtcaaac ccaagtcagg cgtctgggcg ccggcccttg cggcgcgcgc
     4381 cggcaccaca gcggacccaa agtacaagcg cgtgcctctg gagttctcgc cttccatagg
     4441 aatgaccatc aaccatgagc gcgtacgtcc ctggaagtcg cacatgagca actgattttc
     4501 acgccgttcc tccacgctcc acgcggcaaa cgaatcaact gctcctgaag ccagttgacc
     4561 ggcctggacg tccgtcgagg gtttcgaaac gagccatgca agcagcagcc tttcaagcct
     4621 gaacaggggc gttgtgtaaa aggcctggac atactcggcc tgggaaacgt gccgcgctat
     4681 ctccgtggtg tagcaatccg cgtacgcccc cccgtattgg tattttctca ggagcgcgcc
     4741 gtgcgggagt tcagatcgtt gaatggatgg catgtagtgg atgcttggag tgcgaagcct
     4801 gacgtgaaaa tgaccggacc accacccttt gcggcgacgc cgcctccggc agttgcgggt
     4861 gcgtgtcgag cgacatgtta tgcgtctgga ctctcatcac gcgatgacac actccactac
     4921 catccaaaga tactcgcggt tgcgaggtcc tacaagttga gacgtttttg atcgagccgc
     4981 cccagatgga cagagtttcc gagcgataac ttccctctca gcgaacagcg caagctccgc
     5041 cctgatactc tccgatcccc cacgtttcaa gatttcgaag tattgtttat tgccgacctt
     5101 cactcggaaa gcgcgtgcat taaccgcctc gaccggcagc catccaaacg aggagtttgg
     5161 aactgaggtc gcaggaggag gaacataggc gactggttct acgatcacaa agtctgtatt
     5221 cggtggagac gaggtgcacc cgatcaaggc ttcgagcagc aatgccaggg tcaatgaagt
     5281 gaccgacgcg gacaagctag gacgcataac gtcggcgctg tacggcgggc agcgtacgcc
     5341 gcgcagcggc cttctgctgc tgcatgtccg cgacgagcaa caggttaaac atcattgtgt
     5401 gcccatgact catcaaccag cactttgaag taagaccaga gattgtcttc tgctccgttt
     5461 gtcagacgct tattcacttt tgccggtgga aagacccaac tctctccgcg cacaatcccc
     5521 aacgcttggt actcttgatt aattgcccag tctccgcctt tagagcgccg atagacctca
     5581 ccgagataac caccaagcat tttgcaaacc gtttgaattt cctcgtccga cggagacttc
     5641 cgtagtagct tagcaattac gcctttaggc cgcgccttct caagttgttc ggctacaaat
     5701 tcaacctgtt ggacgcttgc agttgaatag tctagcgaaa cagcaaattt ttcacgcgca
     5761 aagtccactg catcctcagc gtacgcagcc atcatttcgt tgattgtcgg ggcagccatg
     5821 tttgatgttt aacattgatt ggacgccaac ggcacccagt ggtccgaagg aaaactaaga
     5881 cgttgcctgc cgttggcggt ccaatcgatc aaccagttaa acttcatttt tttacctgcg
     5941 atctgactga ccgatgcgct cgttcaactc tttgaaatcg gcgatgagtt tgtcacatgc
     6001 tgctttttcc gcttggcaca aggcagacat ctcaatgaat atgccaccat cggtcttcat
     6061 ttttatggct cgacgaacta cggcgggata ggcactgtgg ctacttggtg tgaatgacca
     6121 ttgaacatcg cctggctcat ttgcaatcgt ccaggcatca ggctttgtag tttggatttg
     6181 aacccccggt ttggcctgca gcgcttcaag cgcagcttga acagttggat aaccaatgcc
     6241 tggactcaaa gcggacgggg ctggttcggc cggaacaaca gattgagcaa aagctgaggc
     6301 tccgacaaag cagaacagca gcgaggcaag gtaacgcatt tgagcaaccc tcggtgaggt
     6361 ttaacgctgg agatgaccgg tggacagtag gtcggcgaag ccggccaata ggacgtccgt
     6421 gtcgatcaac ctgttagatt gcggggcgct cactttccct cgcccgccca cgagggacgt
     6481 ttcttcacag gcttcgattt ccattcgtgc ccattcgaat cgctaacttg ggccacgatt
     6541 ttgtcccagt acttagcgta tacgtccatg gcgtcctggg tcacgacgta gatttccgac
     6601 ggtgcggcct cggagagtgt tttgcctcga atgccgctac tgatcagaag gtgggtgtcg
     6661 tccagtccct tcggcaggcg aagagcgacg tggctcacgt ggatgggacg gcgtccgacg
     6721 ttcgtgacag ttacgacacc ccagaacttg ttctcgtcgt actccggcgc gccaaaactc
     6781 ttcatgtccc attgaagcga tacctgaaca cttgcacgat cgcgcaggta gttcatcacg
     6841 ccaaggacgg tgcccgtaac gccagatgca agacccagga ctgcgactac ttcggtgatg
     6901 gtcatagagc aatctaacgt gatgtcgacc gcaaaccctg cggtataact tggcgaatgc
     6961 ggttcaactt ggtcatcaaa atttcctgca aatggcgcgg ctgttgggtt ttaccgcaat
     7021 acgctgcaaa ttcatacagg gataaaaaat ttgcgaaaca ccgcaaattc acattttgtg
     7081 cttcctgagg tgccggtcag gcaaattcaa tagtcttttt ggcctccagc ccaatggaga
     7141 cgggcgcaag cagctattta tttggtagcg aattgccatg gttcgccgga ctcaatccgg
     7201 actttgtcaa acatcatgcg aataaacgat gaaccccatg tgttgggcca ccttgtcata
     7261 aagaagccgg ccggctgaat tggtggtgtg tgtttgccag tacacccgtt tgatgccggc
     7321 ggccttcgcg cgctcgtaca cgccttcgat cagcgcgcgc ccgacgccct ggccgcgcgc
     7381 ggccggatcg gtgaagaggt cctgcaggta acaggtgagc tcgatgcggg tcgtgctgcg
     7441 gtgatacagg tagtgggtca gcccgagcaa ttgcccatca agctcggcca ccagcgcaaa
     7501 gacaggctcg ttagggtcga agaaacgctg ccaggtggtt tgcgtgatct ccggcgccag
     7561 cgcggtcttg ccctcgcggc cgtaaaaggc gttgtagccg tcccagagcg gtgtccaggc
     7621 cggaaagtcg gtttgttcaa tcggacgtat gcgaagggga gaggtcatgt ctgaagggtg
     7681 gtgagtttca ggttcagtgc agtgagcggg tcttcaaggc atccggagca accacgctgt
     7741 cgtcctggta gacccgcgcg agcagcaggc tgagggcgta ccggcgtgcc ggcggcgtgg
     7801 cgcggagaat gccctcgtcc tgcaggatct tgatcagttt tgtcggggcc acgcgtttgt
     7861 cgcccacgat ctcgacatgg cccgcatcga cttccacccg gcgcgcccgc gtgtcggttt
     7921 ctatccgggc gttgcgctgc tccgccagcg agaaaatttc gtcaccttcc gcgcagcgcc
     7981 cgccctcttc gcgccggcag gtgccacgct cgttggtgtc tatcttcagg tagttggcga
     8041 cctgttgcgc cagagcgccg ggctcgcgga tgaacagcga ggaataggtg atgtcggccc
     8101 atttcgggcc tgtcaccagg taacagctca gcggcaccat gcccagcgtg agcgggtaga
     8161 gccaggtctt gccgcaggtc ctggccgcgt cgatgccggc aaaaggcccg gagatggtca
     8221 ccagacgcac atcgacagcg cggccggagg gcgccgtggc ggaagattcc gtcatcgacc
     8281 tgcgtgcgat cagcgcgccc tggctgtggc ctatcaccgt gatctggggg acccgggatt
     8341 gcgccgagag ctggctgacg gcctgcttca gctcgtcggc aacttcggcg agcctggcac
     8401 ggtcgtcata ggtaaaacaa gccgtctgct ggccatgaaa agccagcacc tgggccaggc
     8461 cccgaaactg gccggaggag ccgaagcagc cgtgcaccag gatgtggacc ggctggctcg
     8521 cgtcgaggtg caaggtgcga tccgggttgt ccgtgcaagg ccccaggccg ggaatgttga
     8581 ggctgatgtc aggcgacggc aggcggcccg gctccaggtc cacggtgttc atcgcctgtt
     8641 ttgaagcgca gccggccagc aggccgagaa gggcgatgag aatggcgatg tgtttcatgt
     8701 ctttctttca agcatggcgc cggctcatgc gagcgccccg tacaccggct cgcagcgcac
     8761 gtcggtcttg accgagttgg cgatgaagca ggcgtcgtgt gcctcgtggt gcagactgtc
     8821 cagctcttcg cgggtcggct ggcgcgcgcc ggaaaactcc acgtcgggcc gcagggtcac
     8881 gacagtcatg gccatttttc catcggcatt tttttccatg accccttccg cggcgtcgaa
     8941 ataacggtcc acgcagaatt tgcgtttggc cgcgatcgac agaaaccaca acatgtggca
     9001 gctcgacagc gaggacacaa aggcttcctc cgggtccagc gccgaggcgt cggacatcgg
     9061 cagcggcacc acatgcggtg acgacgaacc gggcacctcc aggccgccgt caaaacgcag
     9121 caggtgccgg cggctgtagc ggttgcccgc aaagtcgcct tcgttgcgca gccataaaac
     9181 ttcagcggtg tagtgagcca tggttatttg aagaaggtgt gcagggcgtg cgccgtttcc
     9241 agcggcagct cttcgggaat gaaatgcccg gccggcaagg cctggccgct gacgatgccg
     9301 gcgcattgcg cctgccagag gtccagcggc tggaacagct gctgcaccac accgcgctca
     9361 ccccagagca cctgggtgtc gcaggcgatc ttgtcgccgc gcgcccggct ttcgcggtca
     9421 tgcaccaggt cgatgccggc gctggcacgg tagtcctcgc aggcggcgtg gatcgcctcg
     9481 ggcgtgcaga agcaacgttc gtactcgatc aacgcctcgg gttcgatgta gctcaggccc
     9541 cggctgcccc agccgccgag cttggcgtgc aggtaggcca aggcgcagcc gccaatcatg
     9601 gtttcgggca gcgggttggg ctggatcagg tggaaccagt ggtaatacgc ggtggcaaaa
     9661 cgcatgtcgg tggcgttgta catgtccagc gtgggcgcaa tgtcgatcag gcacagcttg
     9721 tccacgcgcc cggggtggtc cacggccagg cggtgcgcca cgcggccgcc gcggtcgtgg
     9781 ccgcacaggt agaagctgcc gcggcccagc gcgtccatca cggccacggc gtcctgcgcc
     9841 atgttgcgtt tgctgtagtt gctgtggtcg ggcaggccgg ccaccttgga cgagtcgccg
     9901 tagccgcgca ggtcgggcag gaccaggaaa aaatgcgcct gcagcgccag cgccacgcgg
     9961 tgccacatca catggctttg cggaaagccg tgcagcagca atagcgtcgg gccggcggca
    10021 ttgccgccgt agcgggcgaa gatggaggtg ccgttgactt cgaagtcgcg cgcctcgaag
    10081 ccttcaaacc aggcctgcgg cagggtggag gccatgctca gtgcccgccc tgtttccggc
    10141 gcgccaggta ggtgtgcagg ttggccgccg ccgttttgcg taccttgctg cgcagaaagg
    10201 gcgtccagcc cagcaacagg cccggcgtgc ccagcgcctg gcgcgcccag gcccagaagt
    10261 cgaagcggtc acggtgggtg gcgatcagcc cctgcggcgt gaaggtgaag cgcccgtcga
    10321 tcacgttgtg caccatgcgc ccggtggcac tgaagcggta gtgggcctcc cagtgcgcct
    10381 ggccaccggc cgcgtcggcc ctgacgtcgc tgtagtcgag tttccacaca tcgcgcccct
    10441 tggcccgggt ggcgtcgcac aacatgtgcc acatgcccgt gacctgctca tgcccgcgca
    10501 gcgaaaaggc ctcgtcgtca aaggacgcgt cgggcgcata acactgggcc atggtgtccg
    10561 ggtccaggcg cgcaaaggcg ctgtagaact tctccagggt ctgctggttg gggtgcataa
    10621 acactttcgg acgggagggc tcgcggggat ctactcgcga aatcgtacac cctccggctg
    10681 cgcctgcgtc gtgacctgtc gccgacggca ggcacccggg cgacaggccg ggcattgata
    10741 atgcgccaac gcgacccatc tgctccacca tgatcccttt ttccgttctg gacctctccc
    10801 ccatcgtgga gggcagcgac gccgcgcagt ccctgcgcaa taccctggac ttggcgcaac
    10861 acgccgaacg ctggggcttc cagcgctact ggctggccga gcaccacggc atggccggca
    10921 ttgccagtgc cgccacggcc gtcgtcatgg cgcatgtggc cggcggcacc tcgcgcatcc
    10981 gcgtcggtgc cggcggcatc atgctgccca accattcccc gctggtgatt gccgagcagt
    11041 tcggcacgct cgaatcgctg tttcccggcc gcatcgacct cgggctgggc cgggcgccgg
    11101 gttccgacca ggcgacggca cgcgcgctgc ggcgcgacct gaacgccgac cccaacgagt
    11161 ttccgcagga cgtggtcgaa ctcatgcatt acttcgagcc ggtgtccgag ggccagcgcg
    11221 tgcgcgccgt gcccggcgcg ggcctgaagg tgccgctctg gattctgggt tccagcctgt
    11281 tcggcgcgca gctggcggcc cagctcggcc tgccgtttgc ctttgcctcg cactttgcgc
    11341 cggcgcagat gatgcaggcc atcgaggttt accgcagccg cttccgcccc tcggaacagc
    11401 tggcgcgtcc ctatgtgatg ctgggtttca atgtgtttgc ggccgacagc gacgagcagg
    11461 cgcagctgct gtcgtcgtcg atgcagcagg cctttgtcaa cctgcgcagc ggccggccgt
    11521 cgcgcctgca gccgccggtg gccggttacg aggagcgcct cacgccgccc gagcgcgcca
    11581 ttctggaaca aacgctgtca tgctcggcca taggcgcccc gacgacggtg cgccatgcgc
    11641 tggaggcctt cgtggcccgt accggcgccg acgagctgat gatcacctca cagatcttcg
    11701 accacaaggc gcgcctgcgg tcttacgaga tcacggcggg cctgcaggac taagcgcact
    11761 ttcaagagtt gaaatgcaag ccagggccgc gggtccggct ttgccggccc gcaggcgcag
    11821 cggccccctc ggg
//
LOCUS       DAIDFG010000064        11757 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_64_length_11757_cov_19.807298, whole genome shotgun sequence.
ACCESSION   DAIDFG010000064 DAIDFG010000000
VERSION     DAIDFG010000064.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 11757)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11757)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11757
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64_length_11757_cov_19.807298"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2370 a         3440 c         3602 g         2345 t
ORIGIN      
        1 acaaactcgc cggtggcctc gatgttgcgc accgtgtcct tgtagccgat gctggcaaag
       61 cccacgatgg gcggcacgta gttgaaggcg ttgaaaaagc tgtagggcgc caggttcagc
      121 gcaccggccg cgttgtgcga cgagatccag ccaatcggcc gcggcccgac gatggcattg
      181 aacgggtcat gcggcaggcc gtggccgtgg cggggttcgt agaagtggat ggcgtctggc
      241 aaggggaaac cctgaagaaa tgaaaagatg gaacaacgat acctgaagcc acgcgccttc
      301 caggcctgcg ggccatgcgg cggccgcagg ggaatagccc tacgccagtg cggcgatatg
      361 gcaaaaagtc actgaaaaac ccggaactcc tgcgcacgat gccgggcatg attctcgtaa
      421 ccaagtgtta gggacggcag caatctccaa tggcaactct tattccggca atcagcagct
      481 gcggttcccg catgaccagc ggcgagcgca ggctggccga gcggctggag cagaagctcg
      541 acgacgacta cctgctctgg tacgacgtgc cggtgggtcc ccgccatttg caccctgact
      601 ttgtggtgat gcatccacga cgcggcatcc tgatcctgga ggtcaaggac tggagcccca
      661 agacgatcca gcacgcggac aagcaaagct ggcgggtctt tgtcgacggg atgctcaaaa
      721 cccagcccaa tcccgccgaa caggcccggc aatatgcaca cgcggtcgtt gatgcgctca
      781 agcgcgaccc gcaactggta caggcagagg gcaagtacca gggcagcttg agctttccgt
      841 ggagctatgg cgtggtgttc cccaacatga cgcgcaggca attcaccgac ggcctgctgg
      901 atgaggtcat tgaaccgcat tacgtgatct gcagcgatga aatgctggag aaggttgacg
      961 ccgaagactt gcagagccgc ctgtggggca tgttcccctt catgatgagc ggcgtcatgt
     1021 cgctgccgca actggaccgg gtgcgctgga tcatgtttcc cgaagtgcgc gtgcagccgc
     1081 agggcgcgct gttcgacgac agcgatgaaa gcgccgagat gcccagcatc atgcgcgtga
     1141 tggacatcca gcaggaacaa ctcgcccgca gcctgggcga cggccaccgg gtgattcatg
     1201 gcgtggcggg ttcgggcaaa accatgatcc tcggctaccg cgccgaatac ctggcgcagg
     1261 ccagcaccgc caacagcaag cccatcctcg tcctgtgttt caacgaaccg ctgggcgtga
     1321 agctgcacag cgtgatggac gccaagggct tgagcggcaa ggtgcatgtg cggcattttc
     1381 ataaatggtg ccgcgaccag ctggtggctt ttgggcagcc cctgccgggg cagatggggg
     1441 cccagcagtt tgtggaagag ctggtgcagc gcgtcattgc cgccgtcgac cgcaagcaca
     1501 tccccggcgg ccagtatcag gccgtgctga ttgacgaagg ccacgacttt gcgcccgagt
     1561 ggctcaagct ggtgacgcaa atggtggacc cgaccaccaa caggctgctg cttctgtatg
     1621 acgatgcgca aagcatttac gaacgcagcc gcagcaaaca gttcagcttc aaaagcgtgg
     1681 gcgtgcaggc tcagggccgc accaccatcc tgaaaatcaa ctaccgcaac accaagcaaa
     1741 tcctgcaaac cgccaacctg attgcggcag acctgctgac ggcggacgac aaagacgacg
     1801 acggcattcc gctggtcaaa cccatcagct gcgggcgcga agggcaagcg cccatcatca
     1861 tcaagctgcc cagcctgcgc gaagaagcat ttgccattgc cgaccagctg agccacgccc
     1921 accaggaagg ccacgcctgg ggcgacatgg ccattttgtg cgcagactgg aaaaccatgg
     1981 acatttgcgc cgacgccctg caccagcgca agctgccctt caatgtgcgc aagtactcag
     2041 gcgactacaa ccccgcagca gacgccatcc agatcatgac catgaaggtc agcaagggcc
     2101 tggagttccc cgtggtggcg ctgcccggcg tgggacacat gcccgcgcct ggggaagacg
     2161 agcaggaggc cgcgcgggtg ttttatgtgg cggcgacgcg ggcgacgcac atgttggtga
     2221 tgggggtgtc tggcaatagt gggtttgggc ggcggctgtc gacttaaatc cttgcgcctc
     2281 ttcagaagat tgcctaacta aaaaatcaaa tgtcatctac tcgcattact tctgtacatt
     2341 tcaagagata caaggcattc gccaatttca gcatttcgct gcaagatttc aacgtccttg
     2401 taggccccaa caacgcgggg aaatccacga tcatcggtgt ttttcgaata ctctcggaag
     2461 gcttgcgtag ggcgagatcg cgaaaagccg agcagttgat cgccaatgga attagaagat
     2521 ggggacatcg ctttaacatt gctgatttgc cgatttcatc agagaatatt tttcatgact
     2581 acgatgactc agaacctgca gaagttacgt tcaaactttc aaacggcaat aagttaattc
     2641 ttcacttccc agaacaagga tcatgctttt tacttccgga ttcggaaggc ggaaaagtag
     2701 attccccaac tattttcaag aagaaatttg acattagtgt cgggtttgtt ccaattcttg
     2761 gcccggtcga gcaacacgag ttgttgtatc aagaagaggc tgctcgacgc gcattaatga
     2821 cgaacggcgc gtcgagaaat tttaggaata tctggcagca ctacccggac ggatttgatc
     2881 gttttagaag cctcatccag agcacatggc ccggcatgga catagaacgc ccggagattc
     2941 agcagggcag cacatctcaa tcgagagttc taaaaatgtt ttgtccagag gagagatttc
     3001 cccgtgagat ctgttgggca ggttatggct tccaagtctg gtgccaaatg cttacgcaca
     3061 ttgtgaagtc tgctggcgcc gacctactag taatcgacga acccgacatc taccttcatg
     3121 cagatctgca aaggcagtta gttgctttgc tccgagatct agggccagac attttgatcg
     3181 caacgcactc aacagaaata atctcagaaa cagaacccag tagcttgctg acgataaaca
     3241 aaaagaaacg ctccgcaacg agggtcaaag acgtcagtca aattaaacgc gtcttctttg
     3301 cgttgggctc aaatctgaat ccgacattga cgcaaattac gaaaacacgc cgagtaattt
     3361 ttgttgaagg acttgatttt gacatcctcg ctcgcttcgc tcggacaatg acatttgatc
     3421 gtctggcaaa tagaactgac tttgcattag ttaagcttga cgggttcaat cctaggcgga
     3481 tggaggatct ggccgcaggt atagagctaa ctatagggtc gagaatgatg cgcgcggtag
     3541 ttcttgaccg tgactatcga tccaaagaag aagttcaaca aattgcaagt gaactgaaga
     3601 agcacgcgtc acttatatac atccaccagt gcaaagagat cgaaaactat ttattgagct
     3661 cgaaggtatt atctagcgcc atctcctcta ggcttaacga gcgagtacgg cggaccggtt
     3721 gtgcgttgcc tccgctccca gatgtttctg cgctacttga cgaaattacc gaggggctta
     3781 aaggagaagt actgtcgcaa attgtggccc gtaatttgga catggcgaag aaaagccatg
     3841 ctagtttgga cgcggcgacg gtaacacgtg cagcgattga gggctttgag gaggtttgga
     3901 agactccttc gtcacgccta accattgtcc ctgggaagga agtcctggcg cagttaaata
     3961 cgcgcttgca aaaaatgttg tctatatcga ttagcgacgt tcaaatcgca tcgcattttt
     4021 cggtcgacga tatcccccat gatttgcagg agttgatatg tactttggag gaatttagga
     4081 acaccgccgt ctccggggat acataacgct ggatcttttt ggcatctatt accatctccc
     4141 agttccaaac cgcccacggg caccccacgg agacaaataa ttttcagtca tgtcatgatc
     4201 ggcgttaact acctcgaagc ctcaaagaag ttttatgacg ctgtgctcgg caccttgggc
     4261 attggccccg gcgtcgccaa caagaaccgc tatttctacc gcagccccgg cggctcgttt
     4321 gacatcacca cgccgagcaa tggctagacg gcttgcaata gcaacacgat cgggttttag
     4381 gtggcctttg catgaacagg gcgatgccct ccacccagca gaaaagcaat gcgcatgaaa
     4441 tctgcaagcg accaatggtt tgacgaggcg tactaccagc gctactactt cgacgagaaa
     4501 accggcgtgg tggacccggc gcatgtagag cgcctgggca cctttgtgtg cagctacctg
     4561 aagtacctgc gcgtgcccgt gaagcgggtg ctggacatgg gctgcggcat tggcgtgtgg
     4621 cgcgagattg tggccacgca ctttcctgag gcgagttacc acggcgtgga gtacagcgcc
     4681 tggctgtgcg aacgttatgg ctgggagcgt gggtcggtgg tggactacac ctctagcgag
     4741 cccttcgacc tggtgatctg ccaggccgtg ctgacctacc tgaacccggc ggacctgaag
     4801 cgggccctgc acaacctggg ccgcctgtgc cggggcgcgc tgtatgtgga agccgcgacg
     4861 cgcgaggact acgagcgcga cgtcatcgac gatgaactgg ccgacccgcg cctgcaccgc
     4921 cacccggcgc agctgtaccg gcgcggcctg cgcgagggcc tgaccgaaat tggcggcggc
     4981 gtgtggctaa gccgcaagct tgatgtgccg ctgtttgaac ttgaatgcgt ggacagccgc
     5041 tgagcgcggc tgagaaccgt ccagctaccc cgacggcaac aagcgctgcg ccttgcaccg
     5101 gccgagcgcc tgacccggcg accggttgac cccggccagc gcaggggcgc cgtgacgcca
     5161 ggcgcgctcg cagcctatgg tttcgcgggt tgcaggccct tggcttcaac ctgaagcaag
     5221 ccgctgctgg tggctctcag cttgaaggcg ttgtcgatgc cgccaatgaa gaagcccgtg
     5281 ccaacaaaca cgacatcggt tctggacacg gtgacctgtt cgggctcgag gttcaaggcc
     5341 ccgtaaatca gcaggtagcg ttcgttctgg ttggaggggt tgatgaatat cgtcttctca
     5401 tacccatggc cgcgcttctt catgccggtg tgggggtaca ccctgagggg cgaaaagtcc
     5461 gcatccagcg tttcgatgcg catggcgacg tgcgtgagtt cgttgccttt acctgcgccg
     5521 ccagccaggg gaaggctggc gatgctgacg gcatatgttt tcgtgtaggc cggaagctcc
     5581 agcaataaca gtgcctgtgc gccttgtcca aagtcatgag cgggggattt ttcgtccagc
     5641 ctcaactcgg ttttttcaag cggaaggggt accctggcca gcgttttcag ttgctggcaa
     5701 cagggcgccg tgccctgcgc ggcgggtgcc gcgttctggg tggcaagcgg cgcagggtct
     5761 tgcgcgttgc ccaccagatg aacagcggcc aggcaacctg ccgccgccag acggatgaaa
     5821 agatggcgca tgtgttgagg cgtcagtgag cgaacttact ttttcaggtc cgcaaccatc
     5881 tgcttgacca gaatgtcgac actggccgtc aggcaggttc tggttttgtc gatgttggct
     5941 acgagcttgt caatgtcggc aaatttgcaa tcggctgcgg cttcgataaa cacaacatcc
     6001 ttgttgccgc tgttgtaacc gtagtggtag gcctctttgt acaaaacagc ctgagatttg
     6061 ctgtcattga gctcggcagt catggccaca aaaggatagt aggcggttga tgcgcctgcc
     6121 gcgagatatc cagcctcaaa ggtcagaaga agaaacgcgt cctggccttt catgactcga
     6181 acggtttctt tggcttcggc gggctttgcg ccccgcttta ccggcacggc gaccacttca
     6241 taccccgcct gccggaggcc cggcaatgct tttgcataga agtcggcggt aagcttggtg
     6301 ttcttggggt ccagcacgtc gttgagcttt ttggtcttgc tggcggtatc cgccgccgcg
     6361 acgagcccac cgatcaaccc aaaactcatg cccggatgat tgaggacgcg gacgccatac
     6421 tcgtccagat cgttccattg ctggacagcg attttcttga tgcctgcgga ggcctcattg
     6481 ttaaaagcct gcttctcaaa tctggtggcg cagccagtga gcagcaacgc gccgaacaag
     6541 acggccaggc acgcacagcg gtttatcaac gagtttgtca tacggtccct caaagaatag
     6601 ttatacgatt tgtgacctga gcagtttagg cgtgcaaggg acacacaggc cctggcatcg
     6661 agcgctgaag agcactcaat gatccaaatg tcttcgttgc acaacacctc cgtgtgcctc
     6721 gtctacttgt ccaacttcat gaccgatgcc ttctacacat tccggcatcg cagcatgctg
     6781 cacctgcata cgccaggccc ttgaagttga cgcgcaaact tgcagaactt tttgcttaac
     6841 gatggccaaa tcccttacca tccaccgata cctttcgcct actcagcacc ccacggagaa
     6901 aaatcaatgt tcagtcatgt catggtcggc gtcagcgacc tggaagcctc gaggaagttt
     6961 tatgacgcgg tgctcggcac cctgggcata ggccccggcg ttgccaacaa gaaccgctac
     7021 ttctaccgca gcccgggcgg cagcttcggc atcaccatac ccattaacgg cgagccggcc
     7081 tgccatggca acggcagcac catcggtttc aagctggagt ccgcggaaca gggcgacgcc
     7141 tttcatgcgg caggcgttgc caacggcggc accacgtgtg aagacccacc gggcattcgc
     7201 gaaagtcacc cgggcaagcc ctacctggcc tacctgcgcg acccggacgg caacaagctg
     7261 tgcgtgattt accggccggc tgcctgatcc gtcaagctgg ggcaggccga gctgccgctg
     7321 aatgaatcac ctgccccaag ctccccacgc tttccctcat gcggtcagcc ttttgcgcag
     7381 cgcgcgcctg gcgcgcagct ccaaaccctt tctggcacgc ggcggcccgc gcggggagcg
     7441 ctgcgccggc tgccggctga ttcccagcca ctgcctgtgc gcgctgcgcc cggtgctgcc
     7501 ggtgcggtcg ggcgtgtgtt tgatcatggc cgacatcgag cccctcaagc ccagcaacac
     7561 gggctggctg attgccgatg tggtggccga cacctttgcc ttcggctggg cgcgcaccga
     7621 ggtggacccg gcgctgctgg ccctgctggc cgatccgcag tggcagccct acctggtgtt
     7681 tccgggggag tttgtggcgc aggagcgggt ggtgacggcg ctgttgcccg ccgaaaccac
     7741 gggcggcacg ccggcgaagc gcccgctgtt tgtgctgctc gacgccacct ggcccgaggc
     7801 gcgcaagatg tttcgcaaaa gcccttacct cgacaagctg ccggtgctga gcctgcagag
     7861 cgagcagctg tcacgctacc gcctgcgccg ctcgcagcgc gacgcgcatt tctgcacctc
     7921 ggaggtgggc gcgctgtgcc tggagctggc cggcgaaacc catgcggcgc agacgctgga
     7981 ggcttacctg gacgtgttca ccaaccacta cctgcgcgcc aagcagcagt tgccagtgga
     8041 catggaagat gcggcgcatc tgcggttgct ggctttgtca aacctgtagt actctttttt
     8101 tatagctgtt tgcgcccacc ccaaaagggc cagaggccga tttggcgtga aggttggagg
     8161 caaacggcca aggcgcgaca atggagccca tcacgcccgg ccttgtaccc gcaccggccc
     8221 ggcgtcaccc ccacctcaag agccccacca tgaccgacga cctgaaccgg aaaatcgaac
     8281 tgaaaaaaat ggagctgtgc gactcgtgcg ccggcatcca gcgcaactgg cgcaaggccc
     8341 cggggcaccc ggagctggtg cagggcgcca accgccagga aaggcgcggc ggcgcgctgg
     8401 tcaccgtcac caagtaccgc tgcgaccgct gcggcacggc gtgggaatac gagaacgaca
     8461 aggccaacct gaaggcgggc tggtcggtgg tgggtcggta agcggcgccc tgcgttactt
     8521 ctgttttgat agctggttgc gcaggcagca caagggctgg cagccgattt ggcttgaagc
     8581 ttgcgctcca gagaccattc gccatgaact cggcctgacc ctcttcagac gcccataaaa
     8641 aaagacaggc caggcctgtc ttttttgttt tggcgtgcag cagcgaagag ctgctgcgtt
     8701 gctatttact tgctgccggc ggctttcacg tcggccttgg cttctgcctt gacggcgccg
     8761 gtttcgactt tggcggtggc cttgtcggct gcagcgttgg ccttgacggt ttccactttg
     8821 gcgtcggcct tgatgatggt cttgtcggct ttggcgtcag cattcacgat ggttttttca
     8881 gctttggcgg tggccttggc ctggtcatct gcgtcggcct tggcggcttt ggcttgtgcc
     8941 ttgactttgg tcgtggcgga cttggtcacg gccttggttt tcttttcagc agccttgact
     9001 tctgcctttt caaccttggt gtcggccttg gcgtccacct tggcttcgct ttgcacagcg
     9061 gctgccacgg acttgtcggc cttggtttct gccttgacca ctgcaggcgt cgcaggcacg
     9121 gcgggggtag cgggcgcagc ggcttgcgca aatgcagcgc cggtcacgag ggtagcaatc
     9181 agggtggcga gtaatttgtt catgagagtc ctttgagtga gcgagttgtg tgaccagatg
     9241 aagtcatgat gatcccgtgt acaacgtgtg gcgcggccac cccgacgacg ccatacacat
     9301 gctgtttcaa gacagcacgc gcgctttaca tggtgttgcc ggtggcggga ttgtcagcgc
     9361 gcggtcggcg caggctgcag ctcgaagcgt ggtgcagacc gggtcaacag gcgggccttg
     9421 gccgtggcgg cgggccaggc caaaggcact tcgtgcatga gcgcggcgat cagcgtgcgc
     9481 atgtcttcgt aggccgactc gtgcagggct tcggcgacga tcagcaccgt gctggtgtcg
     9541 gcgctgacgg ccgacaggct gctctgccgg ttggtccagc ggcgcgcgtg ggcgttgtcg
     9601 gcctcgtcgg caaaaatgat ttcatgggcc tcggggtgct cgacctcgcc ggagaagggc
     9661 tggtaatgct cgaggccggt ggcgtaacgc acctgcaggt cgcccaccac tttggccagg
     9721 tcgaacaccg ccaccggaat ggcaaaggcc gccgagaccg cgttgcacaa gtccaccagc
     9781 ggatgcagcc gcggcagggc gccttccttg cgcaggcgac gcaggagcgc ctcggccgca
     9841 cagcggtact tggtgggctt gagccccatt ttggagaaca cctggcgcca ggccttgatc
     9901 tccggcaact cgccttcgcc gctgctggcc agccgcgccg cggcgatgcc gctgaagcgc
     9961 gccacctgcg ggccgaccgc cgccttgctg ctgatgcccc ggacaaacat ggcacccggt
    10021 accagctcgg ggcactgggt ccagatctcg ttggaatggc tgaaaaacat gcgcttgcct
    10081 ctttgctgcc gatgatgggc cagtcggccg attcccgcaa cgccgcgctg acactcctgt
    10141 ttttatagct gcttccgccc gtgttcattg ggctggcggc cactttcatg cttgacccgt
    10201 cagcgcgtac tgtgcgcggc gaaacgggtg acgtgcaaaa aaaacacaac gtatccattg
    10261 gttccacggc cgctgcaccg cgcgttttgt cctccatttg ggggatgcca aatcgccgcc
    10321 gggccgctac aaccgggagc ataacaactc tcaatagcac gcctgctttt tcaggaccgc
    10381 atcgttcctg catcacgggc ggcagaaagg aaatgggctc atggtattta caggcaaggc
    10441 gtcaccgctg tcgcaacagg gaattgaaac ggtgctcggc aggctggggg tggacgcccc
    10501 tgcgctctgg gcggtgctga cggttgaaac cagtggctgc ggcttcctgc cggaccggcg
    10561 acccaagatc ctgttcgagc gccactggtt cagccggctc acgcatgggc gcttcgatgc
    10621 acaggcgccc gacatcagca gccgcagcgc ggggggttac ggcaatggcg gcgcttccca
    10681 gtatgagcgg ctcacccggg ccatgatgct cgaccgcgag gcggcgctga aaagcacgtc
    10741 atggggcctg ggtcaggtca tggggttcaa cgccggcaag gtcggcgctg ccagcgtgga
    10801 tgcactggtc agcgccagca aggcatcaga agatgcgcag ctcgacgcca tggcggggtt
    10861 catcgagcag ggcgatcttg cgcggcatct gcgcaacgcc gactgggtgc agtttgcctt
    10921 cagctacaac gggcaggatt tccagaagaa ccgctacgac gcgaagctgg cgctggccca
    10981 caaacgttac cagctcgggc ctttgcccga cctcgcggtg cgcaccgtgc agctggcctt
    11041 gatgctgctg ggttatggcg gcgcgcggtt tgtggatggg tggtacggag aggccacgca
    11101 gaaggcgctg gtcaggtacc agcagcgcgc cgggctgccg gcctccggcg tgcccgacga
    11161 cgccaccatg gcgcgcctgt cggcgaacct cggctggtag ggcaagcgtt ttacctgcgg
    11221 cgcgggtcgt tgggccaacg gtctgcgcgg tcgcgcgttc cgtcgccatc acggtcatga
    11281 ccgcgtgcgc cgtcgcggtc ccggtcgcca tggccacggt cgtggtgcga cgcatgcggc
    11341 ggcggtctgt gcggaaaggc gcgcggaacc ggaaccggca ccaccacaac aggcgcaggc
    11401 cggctgtagc cccgcgggta gtcataagca cggcgctggt agccgccttc gatgtacacc
    11461 ggctgctcca ccaccatggg cgggccaccg ccatgggtcg ggtaagtcgg gtaggccggc
    11521 accgggtaaa cggcgcagcc cgacaggccg gccacggtca aaaccagaag agtcgttttc
    11581 atgatgtgtc ctgacacccg tcctagggcc tgttcacact gtttatacaa gtgcgaacgt
    11641 ggttaaaaca acgccaatct aggcgcgcga cgacgctcag cctggacggc tgggctagga
    11701 gtgcaacgac gagtggcgtt gttttaacca cgttccctcc tggttgcggc caaaaag
//
LOCUS       DAIDFG010000065        11617 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_65_length_11617_cov_17.786715, whole genome shotgun sequence.
ACCESSION   DAIDFG010000065 DAIDFG010000000
VERSION     DAIDFG010000065.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 11617)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11617)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11617
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65_length_11617_cov_17.786715"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2000 a         3755 c         3708 g         2154 t
ORIGIN      
        1 ggcttgtcga aaatgcgcga gaggatgtcg ccgggccgca tgccgccgcg cgccaggcac
       61 agaatggtgt cgaactgcca gtcggactgg tggatcttga tcgccagttt ttcaatcagg
      121 ttgtggtact cgtcataact gacataaagg tgcttgccgt cttcggtcaa catgaaaatg
      181 ctcctgattc aatagctggt tgcgcccgcc ccgactgggc tggagcacga tttaactgaa
      241 aaccctgcaa aaaacggtca ggccacgaac ggatggcgca acaagatcgt gtggtcgcgg
      301 tcggggctgg tcgagatcat gtggatcggc acgccggtga cttcctcgat gcggtgcagg
      361 tagcgctggg ccgcggccgg cagcttgtcg tagtcggtca cgccgaccgt gctttgtgtc
      421 cagcccggca gggtttcgta aatcggcttg cagcgggcaa tttcttctgc gcccatgggc
      481 aggatgtcgg taatgtggcc gtcgagttcg taaccggtgc acagttcgag ttgttcaatg
      541 ccgtcgagca catcgagctt ggtgatgcac aggccggaca ggccgttgac ctgcgccgag
      601 cgcttgagca gcgccgcgtc gaaccagccg cagcggcggc tgcgcccggt ggtcacgcct
      661 ttttccgcac cgacggtgct catgtggtaa cccggcgtgc ccggtgtttc ccaatcgagc
      721 tcggtcggga aaggcccgcc gcccacacgg gtgcagtagg ccttggtgat gcccaggatg
      781 tagtgcagca tgccggggcc gacaccggcg ccggcggccg cattgccggc cacgcaattg
      841 ctggaagtca caaacgggta ggtgccgtgg tccacgtcga gcagcgtgcc ctgcgcgcct
      901 tcgaacagca ggttcgcgcc gtcacggtga gcgtcgttga gttcgcgcga gacgtcggcc
      961 atcatcggct tgagcagttc ggcgtggcgc atggcctcgt catagacggg ctgaaactcg
     1021 atggccggcg catggaggta gccggtcagc acgaagttgt gcaggtccag cagctcgcgc
     1081 agcttggcgg caaaacgctc ggggtacttg aggtcctgca cacgcagggc gcggcgggcg
     1141 atcttgtctt cataggccgg gccgatgcca cggccggtgg tgccgatctt gacggtgccg
     1201 ccggtttcgc ggcgggtctc gcgcgcaatg tcgagtgccg cgtggaacgg caggatcagc
     1261 gggcaggctt cgctgatgcg caggcgcgaa cgcacctcga caccggcttt ttccagccct
     1321 tcgatttctt caaacagctt ggcggccgac agcaccacgc cattgccgat gtaacacttg
     1381 acgccgggac gcatgatgcc gctggggatc aggtgcaggg ccgtcttgat gccgttgatg
     1441 accagcgtgt ggccggcatt gtggccgccc tggaaacgca caaccccctg cgagctttcg
     1501 gtcagccagt ccaccagctt gcccttgcct tcgtcacccc actgggtacc cacgaccacc
     1561 acgttgcggc cggcggttgc ggcgattgtc tttgtcatct tcagtctgct ttcaggaggg
     1621 gttggttatg cggacggcgc ggcttcaggc cggcttgttg cccagcggct ggacctgcca
     1681 ctggccggcc acgctggcca gttcgcggtc acagtcaaat tcatcaactt cgtgctcatg
     1741 gccgggcaac acgcaggcca cggtttcacc gccggcgcgc agtgcggcaa tcgcgctgcg
     1801 cagcccggct tcttcgcccc agggggcgcg gatggcggga cgcaagggtt tgggtgacag
     1861 cacgctgacc agttctttca ggtccaggct gaacccggcg gccggacggt tgcgcccgaa
     1921 cacagcgccg acctcgtcat accggccgcc gcgcaccagc tcggcgccct gggcgccgcc
     1981 ctgctggccg tggccataaa tcgcaaaacg ggtgccgctg tagtaggcat aaccgcgcag
     2041 gtcagccagg tcaaaaccga tgctgacatt gctcgccagg ctgctgacat gggtggcgag
     2101 ccacttcaaa ttctgcagcg ccttgagcgc ttcgggcaag gccttgaacg ctttttcggc
     2161 ctcaagcaat accttctcgt cgccataaaa ctgcagcagg gcgagcagtc cctggcgtgc
     2221 cgcggcgggc aactcgccgt tgaggctggc ggtcaggctg gccagctcac tggcgtcctt
     2281 gctcgccagc gcggcatgaa tgcgtgccag catggcggca ctgactttgg cccctgccag
     2341 caggctgctc acgatacgca catcggccat atccaccgac agcttcgtga cgccggcggc
     2401 actcaagcct tccagcgcca gcagctgggc ttcgaggtcg gcttccagcc cggcatggcc
     2461 gtagatttca gcgccgaact gcagcggctc gcgtgtggcg tgcgggcggt cggcgcgggt
     2521 gtgcagcacc gggccgcagt aacacaggcg ggccacgccc tggcgattga gcaggtgggc
     2581 gtcgatgcgg gcgacctgag gcgtggtgtc ggcacgcagg cccaaggtgc ggcccgagag
     2641 ctggtccacc agcttgaagg tctgcaggtc cagcgcctca ccggtgccgg tcagcaggga
     2701 ttcgagatgt tcgagcagcg gcggcatgac cagctcgtag ccgtagctgc gcgccgtgtc
     2761 gagaaacagg cgacgcagct cttcgatgtg acgcgcctcg gagggcagga catcggcaat
     2821 ttgatccggc aattgccatg atgacgacca ggcgggcatg tatttatcgt gggctgttaa
     2881 aaacaggatt ttaccgggct tggggcctgt tttcggcccg cgtcggcaca cagatgcccg
     2941 gcgggctcaa atacaggcgc cagaccgccg tgtcagcggc tgctggtcag agcagaaagg
     3001 ccatccacgc caggcctgca acgatgctgc ccagcccgaa aaagcggatc tgcccgtccg
     3061 tgagttggag gatcttctcg aacatgccgc gccagccgcg cggcgagaaa aacggcagca
     3121 gcccctcgat caccagcatc aacgccagtg cctgccagat cgtgctgccg tccaagaggg
     3181 accgttattt tttgccggcc ggggccgtgc cgctgccgga gctgcgcatg gccttgaaga
     3241 agtcggacga aggatcgatc accatcacgt cgcccttttt gtcgaagctg gccttgtagg
     3301 cgtccagact gcgatagaac tgggcaaact gcgggtcacg gccaaaggcg tcgctgtaag
     3361 ccgcggcggc ctgcgcatcg ccctcaccct tgattttctg cgcttcgcgg taggcattgg
     3421 cgatggtgac ctcgcgctgc cggtcggcgt ccgcgcggat tttctcgccc tcggcagcac
     3481 cggtggagcg caactcattg gcgacccgct tgcgctcggc ctccatgcgg cggtacaccg
     3541 attcggtgat ggcttccaca tagtcgacac gcgtgatgcg cacgtcgatc acgtcaacgc
     3601 cccagggctt ggcgccgcgc acgacttcca gcacctcggc tttcacgtcg gccatcaggg
     3661 cttcgcgttt gagcgacagc aggtccttga cggtgcgctt gttgatggct tcctggaaag
     3721 cattgcgcac gacccggttc agctggttgg caccggcacg ctcgtccagg cccacattac
     3781 ggatgtactc cgagggctgg atgatgcgcc atttgacaaa ccagtcgatc accacgcgct
     3841 gcttctcggc agtcagcatc ggctcggcat cggtgctgtc cagggtcagc agccgcctgt
     3901 cgatgtacga cacgttctgg aaaggcggcg gcaacttgaa gttcaggccg ggctccagga
     3961 tgacttcctt gatctggccc agcgcgtaca ccacgccgaa ctgccgttgg tcgaccacaa
     4021 acagcatgga actgagcaat gccagcgcca ccagcatcga agaagcaata aatccaactc
     4081 tattcacggc agttccttag cgtgtttcgc ggtcgcgggt gcgtgaggtg tcacgggccc
     4141 gcgtatcgag ggtcgaagtg gacggagcga ccgggggcaa agccgccgag ccggccggct
     4201 ccacggtggc ggtcttggct gccgtggtgg cctgcatcag cttgtcgagc ggcagataca
     4261 gcaggttcga gccctggcgt gactccacca gcaccttggt cacattgctg tagacctgtt
     4321 gcatggcgtc ggtgtacatg cggtcgcgtg tcacctgggg cgccttctgg tactccgcca
     4381 gcaccgagcg gaagcgctgc gcatcaccct gggcctgtgc caccaccctg gccttgtagg
     4441 cctccgactc ttccttgagg cgcgatgccg aaccgaccgc gcgcggaatc acgtcgttgg
     4501 cataggcctc ggcttcgttt ttcgcgcgtt cgcgctcctg cccggccttg agcacgtcgt
     4561 cgaacgaggc ctgcacctgc tcgggcggac gcacaccgct ttgctgcaag ttgatgccaa
     4621 ccacttcaac cccgaccttg tagcggtcca ggatggtctg catcagggtg cgcacgcgcg
     4681 gcgcgatctg gtcgcgttcc tcggccagcg cagagtccat tttcatcttg ccgaccactt
     4741 cgcgcaccgc ggtttcagcc gcctgcacca ccgtggcagc tggatcacgc gtctcgaaaa
     4801 gataggcacg cgcgtcgttc aggcggtact ggaccgcaaa cttgatctcg acaatgttct
     4861 cgtcttccgt gagcatggcc gactcacgca ggccggtggc cttgatgatg gtatctcgac
     4921 ccacgtcaac ggagcggatc tgggtcacca cgacgacttc atggcgctgc acagggtaag
     4981 gcaggcgcca gttgaagccg gcgcccacgg tcgagttgta cttgccgaac tgggtgatga
     5041 cagcctgctg gccctcctgc acgatgaaaa agccggtacc cagccagatc agcacagcca
     5101 cgccggcaat caggccgaca ccgatacccg cacttttcat gtcgggctgg aacccgccgg
     5161 ggccgccccc gcctccgccg cccagtccac cggtaccgcg gccgcgttgc ccggcatttt
     5221 tggcgccgcc aaaaagaccg ccgagcttgc ggttgaagtc acgccacaat tcatcgaggt
     5281 cgggcgggcc ctggttgggg ccctgaccct ggccctgcgg acggtttggc gccggcggcc
     5341 gcacaggcgg cacgtcatcg cggccggcct ccggcttgga ctcgtcgggc ggggccttgt
     5401 tgtcgtcgtc gcggccccag cgcggatcat tcaaattgaa cattcctcgg gcgcgttgcc
     5461 gcagccttgc cggcagggtc gtcagcgtct gtaacacagg gttcaggttc atgaaatcga
     5521 gctcactttt ttatgattcc cacattgtgc ctaatcaggc cacggcctca tcgattgggg
     5581 ggatatcttc gacgggcgcc tgcgacgcat gccgggccag caactcgcgc aacaggggta
     5641 caccctcgcc cgtctgtgcg ctgacaaaaa cccggtccac acgcctgccc gtgtccgaaa
     5701 aggcgtcctt gatctcgaaa ctgtcactta gctgcagtgg ccggcgcgat ttttcaaggg
     5761 cgtcgagctt gttgaagacc agaatctgcg gcacgtcggc cgcgccgatc tcggccagca
     5821 cgcgttgcac ctgctcgatc tgctcgagat ggtccggatt tgcgccatcg accacgtgca
     5881 gcagcaaatc ggcgtcagcg gcctcctgca gggtggcggc aaacgcatcg accaggccgt
     5941 gcggcaggtc acggatgaag ccgaccgtgt ccgacagcga caccgatcgg gcagcgtcgc
     6001 caaggtacag ctgccgtgtt gtggtgtcca gcgtcgcaaa caactggtcg gccgcgtagg
     6061 cgcgcgcctt gacgagggca ttgaacagcg tcgacttgcc ggcattggtg tagccgacca
     6121 gggaaatcgt gaaggagttg cgccgctcac gctggcgccg ctgggtctgg cgctgcttct
     6181 tgaccttggt caggcgctcc ttggtgcgct tgatggactc gccgatcatg cgtttgtcga
     6241 gctcgatctg cttctcgccc ggaccgccgc gcacgccggc gccgccggtc tgacgctcca
     6301 ggtgcgacca gcgccgtacc agacgcgtcg acaggtactg caggcgcgcc agctcgacct
     6361 gcagcttgcc tttgtggctg cgcgcgcgct gggcaaaaat ctcgagaatc agcaaggtgc
     6421 ggtcattgac cggcaggcca agcacccgtt ccagattgcg ctgctgggcc gggctcaggg
     6481 actggtcaaa caggatttcg ctggcgcccg tttccagcgc gagcatcttg atctcgtcgg
     6541 ccttgccgct gccaatgaac agcgctgcat ccggcgcccg gcgcttgcag accacacggg
     6601 caaccggctg caggccggcc gtctgggcca gcaggcccag ctcttccagg ccggcatcaa
     6661 aattggggag gcccagatca accccgacca gaatggccag aggcgctgcc gccggggaga
     6721 ggtcctggga actcagctgg ctgggctttc ttgggattct gctgcagcaa aattgactgc
     6781 acgaccagga acaatggtcg aaatggcgtg tttgtagacc atctgggtga cggtgttgcg
     6841 cagcaggacc acatactgat cgaaagattc gatctgaccc tgaagcttga tgccgttgac
     6901 gaggtaaatc gacaccggca catgctcgcg gcgcaaagta ttgaggaaag ggtcctgcaa
     6961 aagctgacct ttgttgttgc tcacgatatt ttccgtgttc aagagttggt aggacttcac
     7021 cttaccacag ccaaacggcg gcggctgtca tcctaaggcg aatctttgcc cgcgaagggg
     7081 ttgctggacg acttcatctg gatgcgcagc ggcgtgccca ccagatcgaa ctccttgcgg
     7141 aagcgccctt ccagaaagcg cttgtaggcg tcggtgacgt gttccagcga attgccgtga
     7201 acaatgatga tgggcgggtt catgccgccc tggtgcgcgt aacgcagctt gggtcggaac
     7261 atgccggccc gttgcgggct ctggaactgc accgcctcga gcagcaggcg ggtcagcact
     7321 ggcgtcgaca tcttgcggtt ggccgaagca tgggcctgga caatcgattt ccagaccggg
     7381 cccagaccct ggcgcttgat cgctgaaata ttgtggatgg tggcgaactt caggaaggac
     7441 agccgcgttt cgatggagcg ctgcaggatt tccttctgat aggcgtccac cgcatcccac
     7501 ttgttgacgg ccagcaccac cgcgcgcccg ctttccagga tgtagccggc aatgtgggcg
     7561 tcctggtcgg tcacgccctg ggtcgcatcc agcagcagca acaccacatt ggcgctttcg
     7621 atggcctgca gggtcttgac caccgagaac ttttcaatcg cctcgaacac cttgcccttg
     7681 cggcgcagac cggcggtgtc gatcagctcg aatttctgcc cgccgcgctc gaagggcacg
     7741 gaaatggcgt cgcgcgtggt gccgggctgg tcaaacgcca ccaggcgctc ctcgcccagc
     7801 caggtgttga tcagcgttga tttgcccaca ttggggcggc ccgccaccgc cagcttgatc
     7861 accgtcgggt cgccaggctc gccctcctcg tccggatccg gcaggttcag cggcttcagc
     7921 gccatctcga ccagggtgcg catgccctga ccgtgcgagg cggacaccgg caggacctca
     7981 cccagcccaa gctcgaaaaa ctcggacagg tgcacgccct ccgtcatgcc ttcggccttg
     8041 ttggcggtca acacggtcgg cttgcccagt ttgcgcaggt atttggcgat ctcgtggtcc
     8101 tgggcattga ccccggcgcg cgcatcgacc acaaacacca cgacgtcggc ttcagccacc
     8161 gcctgccggg tctgcttggc catttccttg tagatgccat cgacggcctt gggctcgaag
     8221 ccgccggtgt cgatgacgat gaactcctgc gcgcccagat ggccattgcc gtagtggcgg
     8281 tcccgcgtca ggccggcata gtcggccacg atcgcatcac gcgacttggt caggcggtta
     8341 aaaatcgtcg atttgcccac attggggcga ccgaccaggg caataacagg tttcacaaga
     8401 gagcattcag ggcggagaaa caagcctcct gggagggcaa cgaagtgaca agcgtagagg
     8461 ccacctctct accagccggg gccgcggaac cggcttctcc gggccgcagg cgcagcgccc
     8521 cctcgggggg cagcgacgac acgtcagtgc ggagcgtggg ggccaccatc tactcgggcg
     8581 cgaagccgaa aacagcgcct gaacgcgtga ccgcaatcag gatattgccg gccagcaccg
     8641 gtgaggccgc caccggcgag ccgtcggtca cgaggcggtt cagcggcgag ccgtccgcac
     8701 gggacaacag gtgtacaaac cctgcggcgt cgccgatggc gatggagcgg cccacgacca
     8761 gcggcgcgct caggctgcgg taacgcaggc tgtccgaact ccaggccctt tccccgtcgc
     8821 tacggcgcca ggccagaacc ttgccgtcgg cttcggtgcc aaacaccagg ctgtcgtcgc
     8881 catcgatgcc ctgcgcgcca ttggcgggct tggtccacag caggacgccg cgctgggcat
     8941 cgacacagcc gacgctggcc tggaaagccc gggcgcagac cgtgttgccg gtgcggctca
     9001 cggtgcccac caagtccacc aggcgctcca catcattgat gccgcgcggc gaggcaatcg
     9061 gggcctccca gcggatgctg ccgttgagcg gattgagtcc tgtcaggcgg ccggccaggc
     9121 ccaccaccag tgtgtcgccg acggcgagca tgacacccga ctggcgcagc accagcggtt
     9181 cgctggggcg ctgctgggac cagagcttgc gcccggtctg accgtcaaag gcattgaccg
     9241 accggtcggc cgcgagcaca aacacccggc cgccggccac aaacggtgcg gtgaaggcct
     9301 gggccgccag tttctggcgc cagatttcct tgccgcccga cacggccacc acttcgttgg
     9361 tccgcgtgac cacagcggcc accttgccgt cgctgcccac gccggctgcg atcggcgcgc
     9421 cgacattcgt gcgccagaag tcgcggccac tgcgtgcgtc cagcgccacc acggtgccgt
     9481 cggtgctcgc cgccaccacg gtatcgcccg agacgttcac ctggagcggg aaagaaacct
     9541 cgccgatacg ggcggtccag acctgtttgg ccggtaccag cgccaccacc ggctgcagtt
     9601 cagccggctg cggcttcttg acggagctgc tgaacatcga gcagcctgcc aaggatgcta
     9661 ccaaaaagat agctgcaggc gctttcagcg tatgggccag aggccgaaaa gacttgaaaa
     9721 tcactgcttg acctccggtg cggacgcggg agaaactgca gcggcggctg cagcaggggc
     9781 caatgccgcc gacgggttca acccgagcgc attgagtttg acttcgacca ggcgccggta
     9841 ctcggtgcgc tcatccatgc ctttccaggc tttttcgtat tcggcccggg cttcgttttt
     9901 cttgccctgt gcggcgagaa tgtcgccgcg gcgatccgcc gccagcgcaa caaagtcctt
     9961 ggggaaggca ccggtgagct gctgcagggc ctcgtcgtag gctttggctt ccagcagcaa
    10021 acccgccaga cgcagcttgg caatcgcctg gtaaccttca tcggcgcttt tgccggccac
    10081 ccagctcagg gaaaccttgg cggcatcgat gttgcccttg tcgtaatagg tcctggccgc
    10141 cagcaggccg gcctgctggg caaaggccgt cccggcaaaa cgctccttca tgtcggccat
    10201 cgcgcggtcc agccggctgg tgtctcccga ctgggcggca cgttcgacct cgtcgtacat
    10261 cacggccgcc ttggcggcct gatcgcgctg ccagtagttg tagccgttcc aggctgcaaa
    10321 agcgccaaaa atgacaatca agccccaggt aatcaggtca ccgtattgct tccagaagtg
    10381 tttcagctcg tcgagctgct cctgttcttc caaatcgaga tgttttgcca tagagctctg
    10441 ctggttccgc caaatgaatt gtgttgattg taggggtcgc tgtcagacgc cagcgtgcag
    10501 gctgtgcgcc cacggggcca cggtctccag cggctgcagg gtttgtgcct gggcggcctc
    10561 gaccttttcg ccgcgcaggg gcttgacggc cacgctgccc tgcgcgagtt cagcctcgcc
    10621 aaagatcagg gcgaaccggg cgccgctggc gtcggccttc ttgaactggg atttcatgct
    10681 gcccatgccg tctgcggagg cgccggaggc cgcatgcatc tgcacgctga cgcccagggt
    10741 acgcaaggcc tgcagggttt tcagcaccac cggcaaagcc gatgcatcgg gaacaatcgc
    10801 ataagcatcc ggcaccggca gatccgccgc cccgccctgc tccctgatca gctcgagcac
    10861 ccgctccacg cccagcgccc agcctacggc gggcgcggcc ttgccgccga tctgctcgat
    10921 caggtagtcg taacggccgc cgccgcagat cgtgccctgc gagcccaggc tctcggtgat
    10981 gaactcgaac accgtcaggt tgtagtagtc catgccgcgc accagccgcg ggttgatgcg
    11041 gtaggcgatg ccgttggcct cgagaatggc cttgacgctg ctgaaatgcg ccagcgatgc
    11101 ttcgcccaga aaatcgatca gccgcggcgc ggcctccacc agggcctgca ttgtcgggtt
    11161 tttggtgtcc aggatgcgca gcgggttgct gtgcagccgg cgtttggctt cttcgtcaag
    11221 ctggtccgcg ttggcctcga aatagctgat cagcgccccg cggtgcaact tgcgttcttc
    11281 gggctgcccc aggctgttga gttccagccg cacgtccttc aggcccagtt ctttccacaa
    11341 ggcatcggcc agcaggatca gctcggcgtc gacttcggcg ccgggaaagc ccagcgcttc
    11401 ggcaccgatc tggtgaaact gccggtagcg gccccgctgc ggacgctcgt gccggaacat
    11461 cgggcccatg tagtacaggc gcttgccgcc gtcgtacagc atgctgtgct cggccacggc
    11521 ccgcaccaca ccggcggtgt tttcgggccg aagtgtcaag gcttcgccgt tgagcctgtc
    11581 ctcaaacgag tacatctctt tttcgacaat gtcggtc
//
LOCUS       DAIDFG010000066        11495 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_66_length_11517_cov_17.216280, whole genome shotgun sequence.
ACCESSION   DAIDFG010000066 DAIDFG010000000
VERSION     DAIDFG010000066.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 11495)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11495)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11495
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66_length_11517_cov_17.216280"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2516 a         3432 c         3297 g         2250 t
ORIGIN      
        1 tctgctcgac gccggtcagt tcatcgcgac actggagcat cgtcagggtc tgaaaatcgc
       61 ttgccctgag gaaattgcct ggcgcaatgg ttttatcggt gatgcccagc tcgagaagct
      121 ggcgcagcca ctcgccaaga acggctacgg gcaatacctg ctgcgcctgc tgaaggagcg
      181 cggccagtca tgaaggtgac ttctaccgtt attcctgatg tcctgatcat cgaaccccgg
      241 gtattcggcg acccgcgcgg tttctttttt gaaagcttca accagcgcga attcaatgcc
      301 gcgactggca ccgatttcag cttcgtgcag gacaaccaca gccggtcggc gaaaggcgtg
      361 ctgcgcggcc tgcattacca agtgacccag ccgcagggca agctggtgcg cgtggtgcgc
      421 ggcgccgtct tcgacgtggc cgtcgacatc cgccccgcat cgccgacgtt tggacaatgg
      481 gccgggttgg aattgaatga agacgatcac aggcagttgt ggatgccacc tggctttgct
      541 cacggttttc tggtgcttgg tgagagtgct gacgtcctgt acaagacgac agagtactac
      601 tcgccaactg atgaagcctg tcttgcatgg gatgatcccg gcattggcat tcagtggcct
      661 ctacgatcgc cccccattct ttctgccaaa gatcgccaag gccgaaagct caaaccagtc
      721 tgatttcctg cgactggcga tagcaattcg tctgcatggg tgtgcgcagg aactcctcga
      781 cgcgtttgat ctgtatccgg cggttgcacc gattcattcg gactttaagt ctctcaactg
      841 caccatgttg gccacgccgg ttccagcatg gtaacatccg tgttcatcga ctgaacagca
      901 agaaattttg ccatcggact gtcgaatacg gtcgttgaaa gagcttcaga atcatgtccg
      961 agtcaaagcg attgctgcct tccccgctcc aggaagaaaa tcatctcaag gttttgcgtc
     1021 tgcttgaggg caatccgcac atgaatcagc gcgacctggc tgacgcactc ggggtgagtc
     1081 ttgggaaaac taattactgc ttgaaggctt tgttggacaa gggcttcatc aagatgcagc
     1141 gctttcgtaa aagccagcga aagttggctt atgcctacct gttgacgcct gtgggaattg
     1201 ctgagaaagc aggtctgacg gcccggtttt tggagagaaa ggtcgctgaa tacgaaggtc
     1261 tcaaagtcga aattgaagct cttaagtttg aagttaacga agcacaagaa aaatcaggcc
     1321 tgacatgacc aacctaacaa tcacccccgt cattctttgt ggcggctcgg gtacgaggct
     1381 gtggccactc tcgcgcaaga gttttcccaa gcagttcgtt cctctgattg acaacaaaag
     1441 cctgcttcag ctgacgctgg agcgtgtagc gcaagccggt agcgacggca cagcagcccc
     1501 cgaggtgatc tgcgttgccg ccgaagagca ccggtttctg gtcagcgagg ccatgcaggc
     1561 ggcaaaagtc aaagggcaag tcattctgga gccggttgcg cgcaatacgg ctgccgcgat
     1621 ggcgctggcg gcgctgacgg ccgcgccgca ggacctgctg ctgttttgcc cctcggatca
     1681 ccacattcct gattcccagg ctttcatggc catggttcag caaggcgtcg tggcggcccg
     1741 tcagggcgcc gtcgtgacct ttggcgtcgt gccgagcttt cccagcacgg cttatggcta
     1801 tattgaacag ggcgctgcca gcggcgatgg cagctttggc gtggcccgtt ttattgaaaa
     1861 acctgccgcc gatcgggcgc aagctttgat tttgcaaggt aatgtgctct ggaatgcggg
     1921 gatttttctg gctcaggccg gcaccttgat cgaagcactg cagcagcatg caggcgatat
     1981 attgcaaagt tgccggctgg ccatggcaca agccaggccg gaccagcaat ttgttcggcc
     2041 tgagcctgag gttttcgccg cttgccgctc cgacagcatc gactacgccg ttctggaaca
     2101 tcataaaaac gtggcggtgg tgccgttttc cggggcctgg agcgacgtgg gcagctggaa
     2161 tgccgtggcg gatttaacgc cagctgacga ccagggcaac cgcattgacg gcttgggctt
     2221 ggctgtccag agccagcgaa catatattca cgcgccacac cgcaccgtcg tcgcgctagg
     2281 cacgcgggac ctgctgatca tagacacgcc cgatgcgctg ctggtggcag ccagcagcca
     2341 tgccgagcag gtcaagcacg tggtggcacg gctggagacg caaaaaagcc cggaagccag
     2401 catgcatcgc aaggtggcgc gcccttgggg ttggtatgac agcattgacc gcggccagcg
     2461 ctttcaggtc aaacgcattg ctgtcaagcc tggcgcatcg ctgagcctgc aaaagcacca
     2521 ccaccgcgca gagcactgga ttgtggtttc cggcaccgcg ctggtgacca aaggcagtga
     2581 gactttcctc ctcacggaga atcagtcaac ttacattcca attggtgaaa cccaccgcct
     2641 ggaaaacccc ggcaagaccg acctcgagat gatcgaagtc cagtccggca gctatcttgg
     2701 cgaagacgac atcgttcgat tcacagacaa ctacggcagg gcctaaagaa tgaaaaaagc
     2761 acttatcacc ggcgtcaccg gacaagacgg atcctatctc gctgaatttt tgctcagcaa
     2821 aggctacatc gtgcacggca tcaagcgccg cacgtcgctg ttcaacaccg accgcatcga
     2881 ccatctgttc caggacccgc atgaacccaa ccagcgcttc gttctgcatc acggagacat
     2941 gacggattcg tccagcctga cacgcatcat ccagcagacg cagcccgacg aaatttacaa
     3001 cttggcggcg cagagccatg tggctgtgag ctttgaggag cccgagtaca ccgccaacag
     3061 cgacggcctg ggtgcactgc gcatcctgga ggccatgcgt attctgggcc tggaaaaaac
     3121 aactcgcttc taccaggctt cgacctcgga gctgtacggt ctcgttcagg aaattccgca
     3181 gaaggaaacg acgcccttct atccgcgcag cccctatgcc gttgccaagc tctatgccta
     3241 ctggatcaca gtcaactacc gcgaagccta tggcatgtat gcttgcaacg ggattttgtt
     3301 caaccacgaa agcccggttc gcggtgaaac ctttgtcacc cgcaaaatca cccgggcgct
     3361 ggcccgtatc aaacttggca tccaggactg cttgtacctg ggcaacctgg actccaagcg
     3421 cgactggggt catgccaagg attacgtcga gatgcaatgg ctgatgctgc agcaggacaa
     3481 acccgaagac ttcgtgattg ccaccggtgt gcagtacacc gtgcgtgagt ttgtcgatgc
     3541 cgcagccaaa gagctgggcc tgcagattcg ctgggaaggc agcaatgagg cggaaaaagg
     3601 ctttgacgcc agcagcggca aatgcatcgt ggctgtcgat ccgcgctact ttcgtcccac
     3661 cgaagttgag accctgttgg gcgatgccac caaggcgcgc gagaagctgg gctgggtgcc
     3721 caaaatcagt tttgcggaac tggttgccga gatggcccag tccgacatga cggaggcaga
     3781 gtttgacctg aaggtcaagc atagcggtca caaagccctg cgcaaccagg gctgagcacg
     3841 gtggcaaaac ctcgcatttt gctcacaggc ggccgcggca tggtgggcca gaacattctg
     3901 gagcaccccg acgccggtaa atgggaattc gttgcaccga cgagccagca gctggacctg
     3961 acggatttca gggccacccg gcagttcgtg gccgacctgc gccccgatgc ggtcatacac
     4021 tcagcgggca gggtcggtgg catccaggcc aacatcgctc acccggtgga ctttctggtg
     4081 accaacgtgg acctggggcg caacgtcatc cttgctgcgc gcgaagcggg cgtcagcaag
     4141 ctgctcaatc tggcaagctc gtgcatgtac ccgcgcaacg cacccaaccc cctaggcgag
     4201 gacttggttc tcaaggggga gctggagccc accaacgagg gttacgcact cgccaagatc
     4261 tttgccacgc gtctttgcga atacatccat cgcgaaacgc cgagcctgca ttacaaaacg
     4321 ctgattccct gcaacctgta tgggcggcac gacaagtttt caccacagca ttcgcacctg
     4381 atccccgcca tcatccacaa ggtgcatcaa gccaaggtcc agaatctggc agaggtggag
     4441 atctggggtg acggctcggc gcgccgcgag ttcatgtatg cgggcgactt ggccgacgcc
     4501 gtgcttcgcg ccatggaaga cttcgaccag ttgccggatc tgatcaacat cggcctgggc
     4561 cacgactaca gcatcaatga atactacgtt gctgcggcgc aagtcattgg ctggcagggg
     4621 aaatttgtgc acgacaccac caaacccgtc ggcatgaagc agaaactggt cgacattggc
     4681 aggcaacgcg cctggggatg gatgccggcc accgccctga atgagggcat agaaaaagcg
     4741 tatcactact acctgcagga caagcacgca tgagcttcaa atatccccta gccaccgcca
     4801 cgtgggacca ggctgaatac gatgcgctcc agcgcgtcat cacatcgggc atgttcacga
     4861 tgggcccgaa ggtattcgaa ttcgaaaagc agtttgcgag ctacctgggc gccaaacact
     4921 gcgtgatggt gaattcgggc tcgtcggcca atctgctgat ggtggccgcg ttgttctatt
     4981 cgaaaaaccc cgacttggcg ctcaagccgg gcgacgaagt catcgtgcct gccgtttcat
     5041 ggagcacgac ctattacccg ctccatcagt acggtttgaa gatcaagttc gtcgacatcg
     5101 acctcgaaac gctgaacttc gatctggacg cgctcgaaaa agccgtgacc aacaagaccc
     5161 gcctgatcat ggcagtgaac ttgctgggca accccaacga cttcgaccgc atcaagtcca
     5221 tcattggcga caagaaaatc acgctgattg aagacaactg cgagtccctg ggcgcgcagt
     5281 tcaagggcaa gcaggccggc acttttggcg tgatgggcag ctatagctcg ttcttctcgc
     5341 atcacatttc caccatggaa ggtggactca tcagtaccga tgacgaagag ctgcaccaca
     5401 tcctgctgtc gctgcgcgcc cacggctgga cccgcaacct gcccaagttc aaccatgtct
     5461 gcggtgaaaa gagcgatgac cccttcaagg agtctttccg cttcgtgctg cccggctaca
     5521 acgtcaggcc gctcgaaatg tcgggcgccc tcggcatcga gcagatcaag aaactgcctt
     5581 cgctggtggc agagcgccga aaaaatgcgg ccatgctgca gacagtcatg caagaccacc
     5641 cggcggtttt gatccagaaa gaaattggcg caagcagctg gttcggcttc agcctagtga
     5701 tacgtcccgg cgtgagcctg actcgcgatg cgctgttgag caagctcaac gcgcttggct
     5761 ttgaagtacg tccgattgtg gcgggcaact ttgccaagaa tgaagtggtg aagtacttca
     5821 attcggaaat ttccggcgta ctcacgaatg ccgagcatat cgacagcaac gggctcttcg
     5881 tcggtaacca ccactattcc attgaggatg cggtcaggca actgaaaggc atttgaacca
     5941 tgaaggcagt cattcttgca ggaggtttgg gtactcgcat atctgaagaa acctatgcca
     6001 agcccaagcc catgatcgag gtaggtggca agcccattct gtggcacatc atgaagatct
     6061 attcagccca tggcgtcaac gatttcgtga tctgctgcgg ctacaagggc tacctgatca
     6121 aggaatattt cgcgaactac tttctgcaca tgtccgacgt caccttcgat atgagcaaaa
     6181 acagcatgga agtgcatcag cgaagcgctg agccctggcg tgtcacgctc attgacacgg
     6241 gcgaggaaac catgacgggc ggacggctca agcgtgtggc atcctatgtc caggacgaag
     6301 aggccttctg ctttacctat ggtgatggcg tgagcgacgt gaacatcacc gaacttgttg
     6361 cttttcacca atcccagaag gtcaaggcaa cgctgaccgc cacgctgccg cccgggcgct
     6421 tcggcgcgct cgacttcgat ggtcacaagg tgcgcagttt caaggaaaag cccaagggcg
     6481 acggcgccat gatcaacggc ggcttttttg tcttgtcgcc agaggtgctc aagtacattg
     6541 acgatgacag cagcgtctgg gagcgcacgc cgctggaaac actggccgat gaaggccagc
     6601 tggccgccta ccagcatgga ggtttctggc aacccatgga cacgctgcgc gacaagactc
     6661 acctggaaga actctgggcc tcgggcaagg caccgtggaa ggtctggaaa tgacacccgc
     6721 attctggcag ggcaaacgcg tcttcatgac tggccacacc ggcttcaagg gcagctggct
     6781 gtcgctctgg ttgcaacagt tgggcgcctc tgtcaccggc tatgccctga gtccgccgac
     6841 atcacccagc ttgtttgtgc aagcccgggt agccgaaggc atgcaatctg tgcacgggga
     6901 cattcgcaat ctgccggacc tgatcaaagc catgcgtgct gcgcagcctg aaattgtcat
     6961 ccacatggcc gcgcagtcgc tggtgcggga atcctacgcc gaaccggtcg cgacctatgc
     7021 caccaatgtc atgggcacag tacacctgtt ggaagccgta cgccagacag caggcgtaaa
     7081 agcagtggtc aacgtgacca cagacaaatg ctacgagaac aaggaatggg tctggggtta
     7141 ccgtgaaaat gagcccatgg gcggctacga cccctacagc aacagcaaag gctgcgccga
     7201 gctggtgagc gcggcctacc ggtcttcttt ttttagttcc cacacccatg cgcagcacgg
     7261 cgttgcgctg gcaacggctc gcgcgggcaa cgtgatcggc ggcggcgact gggccaagga
     7321 ccgactgatt cccgacattc tggcggcgtt tgaaaaagcc cagcctgtgg tgatacgtaa
     7381 cccgcatgca acccgaccat ggcaacatgt gctggagccg ttgcgcgggt atttgacgct
     7441 ggccgaaaag ctctacacgg caggcccggc gtttgccgag ggctggaatt tcggaccgca
     7501 cagcgacgac gcacgaccgg tggagtggat tgtcaggaga ctcgcggatc agtggcctga
     7561 tggcgctagc tggcaaatcg atgctggcga gcacccacac gaggccaact atctcaaact
     7621 cgatatctcc aaagccgccc accggctcaa ctggcatcca gtcatgcgac ttgagcaggc
     7681 actgggcctg atcgttgact gggcccaggc gagacaggcc ggcgctgatc tgcgcgcgct
     7741 cacgcaagga cagatccagg cctaccaggc acaagcattg atttccaacc ccacttgacc
     7801 cccgcggatt ttcaaattat gcagaacgca caaacccaag cccttcgcga ccagatttcc
     7861 gcgctggtgg aacagtacgc cgctcttgcg ctggcgcccg agcctttttt ccccggcgtt
     7921 actgcggtcc cgccttcggg caagctgctg gatggcgcag agctcaaaaa tatggtcgag
     7981 gcctcgctcg acggctggct caccaccggc cgcttcaaca ccgagttcga gaagaagctc
     8041 gccgctttca tcggcgtcaa gcacctgatc accgtcaatt cgggctcgtc ggccaacctt
     8101 gtggcgttca gcacactgac gtcgcccaag ctcggtgaca gggcgatcaa gcccggcgat
     8161 gaagtcattg gtgtggccgc cggttttcca accaccgtca atcccatctt gcagtttggc
     8221 gccgtgccgg tgtttgttga tgtggaccgc ctcacccaca acatcgacgc cagcaagatc
     8281 gaagcagcca ttggccccaa gaccaaggcc atcatgctgg cgcactcgct gggcaaccct
     8341 ttcaacctcg atgtcgtcgc ggccctgtgc aagaagcaca agctgtggct ggtggaagac
     8401 tgctgcgacg cgctgggcac aacctaccgc ggtcagatgg tcggcacttt tggcgatatc
     8461 gccaccctga gtttttaccc ggcccaccac atcacgatgg gtgaaggggg cgcggtcttt
     8521 accaaccacg acgaactcaa aaccattgct gaaagcttcc gcgactgggg gcgcgactgc
     8581 tactgcccac ccggcaagga caacacctgt ggcaagcgct ttggctggaa gctaggcgac
     8641 ctgcccgaag gttacgatca caagtacacc tacagccacc tgggctacaa cctcaaaatc
     8701 accgacatgc aggccgcctg cgggctggcc caactggaca aggcagcgca attcatccag
     8761 gcccgcaagg acaactttgc attcctgaaa aagcgcctga aagactgcga agaatttgtc
     8821 aacctgcccg aggcgaccga acattccgat ccctcatggt tcggctttcc gatcaccttg
     8881 aaagaaaact gtccggtcac gcggcttgat ctgctgactt atctggacca gaacaaggta
     8941 ggaacgcggt tgctgtttgc cggcaatctg acccgccagc cctacatggc aggagcaaag
     9001 taccgcatca gcggtgatct cgccaatacc gacaatgtga tgaacaacac cttctggatt
     9061 ggtgtccaac cttcgctgac accggctatg atggaattcg cagcgcgcaa gatcgagacc
     9121 tatctcggcg tgaacttctg atttgtcatg cgtgctgtct tgccccacga cgatcttgac
     9181 ctggtgttat cgctgacacc ggagttctgg tcgcgctttg gcggagcgag gctgttcatg
     9241 accggcggta ccggcttcat cggcacctgg ctgctgcagg ccatgcagcg cgccaacgac
     9301 aggctcggca gcaaaattga acttgtcgta ctgactcgtg agccagagcg tgcccggcag
     9361 caggctcccc aggtcttcag cagggcagac acctctctgc tggctggcga cgtcagcacc
     9421 tttgccgacc ctgtagggaa gctggacctc tgcatccacg ccgccactga cgtgggcgac
     9481 ctgctcaagg ctggcaaccc catcaaggtg tttgactcga ttgtgctggg aacgcggcgc
     9541 gtgctcgatc tggctcatgc aagtggcgtc tcgcgcttcc tgctgacttc aagcggcgcc
     9601 gtgtatgggc cgcagccagc cagcctggag cgcgtgcccg aaacttataa cggggcgcct
     9661 gacccactgc agtcaaacgc cgcctatggc aacggaaaac gtgcggccga gtggctggca
     9721 gcggctcatg cggcccaggc ttcacaagcc ggattcgagt cgtgcatagc acgcatcttc
     9781 gcgctcatcg ggcccggctt gccgctcaac ggaccttttg cggccggcaa cttcatgcgt
     9841 gacgcgctag ccgggcaatc catcaatgtg cagggagacg gacgcccgct acgctcttac
     9901 ctttacatgg ccgatctctg catctggctc ttgcgcctcc ttggttctgg cgcatcgggt
     9961 caggcttaca acgtcggctc ggaaaatgca gtatcgattg cagcactggc ccggcaagtc
    10021 gctgatgcgg cgggtacgga tgccgccatc aaggtgcaga cgacagccgc gcaagatgcg
    10081 ccgacaccgc gttatgtacc cgacaccttc aaggcaaggc atgagctggg cctggccgag
    10141 tacacagcgc tccataccgc cctgcttaaa accatccact ggagtcgttc ggcgacggca
    10201 ctatgaacaa gtacaacgca aatcaaatcc gcaaagccat tctcgatatg gcttactccg
    10261 gcgccaccgt tcatatcggc tgtgcgtttt ccatcgtgga gctgctggcc gtgttgtatc
    10321 gcaagcacct gcagctcgat cccgccaacc cggctatggc gacccgcgac tacatggtgc
    10381 tcagcaaggg ccacggtgtc atggcccagt acgcctgcct gcatgagctg ggctggctca
    10441 cggacgacga cattggcaac tatttcaagg acggaacccg cctgaaaggc ctggccgacg
    10501 cccatgtgcc cggtgtggaa gcgaccactg gctcgctggg gcacggcttg tcggtcgccg
    10561 caggtctggc gctggcggcc aaactgaata agacggagca gacctgctat gcgctggtcg
    10621 gcgacggcga actcaatgaa ggggccatct gggaagcagc cctgtttgcc gcgcacttca
    10681 agctggacaa cctcgtggtg attgtcgaca agaatggttt tcaggccatg ggcacgaccg
    10741 acgacgtcat cgcgctggga aacatcgagg aaaaattcag ggctttcggc tttgatgcgg
    10801 tcactatcga cgggcatgac gaagcagcca tcgacgcggc ctatagcaac atgaaggccg
    10861 cccgcaacgg caagcccaag gcaattgtgg ctactagcgt caaaggcaaa ggtgtctcgt
    10921 tcatggaaca caacaacatc tggcactaca cacgccttac acccgaaacc tacgcagccg
    10981 ccatcaccga gctggggtac acagcatgag aaacgcattt tccgacgcac tggtagccgc
    11041 tgcgctgtcc gaccccaaag tgttgctgct cacgggcgac cacggttatg cactgtttga
    11101 cgccttccgc aaagcccggc ctgaacagta catcaactgc ggcatcgccg agcaaaacat
    11161 ggtgggcgtg gctgccggcc tggccaaggc cggcttcaaa cccattgttt acgggctggc
    11221 ggcctttgtc cctgtgcgcg tgctcgagca gataaaaatt gacgtgtgtt acgagaacct
    11281 gcccgtcatt ttgatcggcg acggtgccgg cctggtttac ggtcaactgg gcaccagcca
    11341 ccagtccacc gaagacatcg cctgcacgcg ggccatccct tcgctgactg tgctttcacc
    11401 ggccgaccgt tttgaaatga cggcgaccat ggagctcgcc ctgaaactcg cctcaccggt
    11461 ttacctgcgt gtgggaaaaa gtgaccgcgg cgatg
//
LOCUS       DAIDFG010000067        11511 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_67_length_11511_cov_18.928858, whole genome shotgun sequence.
ACCESSION   DAIDFG010000067 DAIDFG010000000
VERSION     DAIDFG010000067.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 11511)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11511)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11511
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67_length_11511_cov_18.928858"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
     assembly_gap    1791..1890
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2161 a         3567 c         3641 g         2042 t
ORIGIN      
        1 gcagcagtag tgggcaaagg acaggtcggc ggccatgggg aaagtctaac gccggtgccg
       61 tgcttcacaa tagcgcatgg cccgtcccaa atccgccacc cacgagctcc agcgcgaagc
      121 catcctcgac gtggccgcgc agtgttttgc acagcgcagc tacatggccg ccagcatgaa
      181 cgacatcgcc gcggcctgcg gcacctccaa ggccaggctg taccactact acggcagcaa
      241 ggaagccatc ctgttcgacc tgctggaccg ctacacccag cacctgctgg ccatcatcgg
      301 tcagaccgag gccagcgccc agcgcaagaa cctggacgac cgcgctgccc tgcacgaact
      361 gatccgcaac ttcctggacc agtacgaaag ttcggccacg cgtcacagcg ccttgctggg
      421 cgacaccaag ttcctcgggg acgtgcagcg cgagctgatc ctggaccggc agcgcgacgt
      481 ggtgtcggcc atgacgcgtt ttctcaagcg cgcctacccg cagcgcatca cgcccgccaa
      541 ccagacggct gtgaccatga tgctgttcgg catgatcaac tggaccttca cctggatgcg
      601 tcccgacggg cccatgagtt accgggacta tgcccaggag gtggttgcca tgcttgagcg
      661 cggactgtcc tgactgctga ctgcggcgca gaagggttgg gggcttttgg gggttttact
      721 attcgtaata agttacgtat agtaaaattt cagcgattca ggccgtgctc tcggcccttc
      781 cccaacggag acttccccca tgtccaaagc tttcgcctcg cagtccgacc tcacggacaa
      841 aaaaatcacc ttcgagcagc tcagtgccca ctgctgggcc tacaccgccg agggcgaccc
      901 caactccggc gtgatcattg gcgacaagtt catcatggtc agcgatgcga cggccacccc
      961 ggccatggcg caggacctga ttgcgcgcat ccgcgccgtc agcgacaagc ccatcaagta
     1021 cgtgctgctg acgcactacc acgcagtgcg ggtgctgggc gccagcgcct acatcgccga
     1081 aggcgcgacc gaggttatcg ccagccaggg cacctacgag ctgatcgtcg agcgcggcgc
     1141 ccaggacatg caaagcgaga tggagcgttt cccgcgcctg ttccgcaatg ccgaaagtgt
     1201 gcccggcctg acctggccga cgatggtgat tgccggcggc aacccggtca agggcgaagt
     1261 ccccggcaag ctggtcatcg acctcggcgg cgtgaaggtg caggtctggc atccgggccc
     1321 gggccacacg cgcggcgaca cgattgcctg ggtggaagag gaaaaagtgc tgttttccgg
     1381 tgacctggtc gagtaccagg ccggcgttta caccggtgac gcccagctcg aagagtggcc
     1441 cgccaccctg gaagcgttgc gtgcgctggg ggccgaggcc atcgtgcccg gccgcggcga
     1501 ggcgatgaaa ggggctgccg aggtgaacaa ggccatcgac tacaccaagc agtgggttga
     1561 aacgctgttt cgttgcggca aggaagcagc tgccgcgaag atggacctca aggccgcgat
     1621 ggatcacacc cgcaagagca tggacccgat ttttggccag gtcttcattt acgaacactg
     1681 cctgccgttt gacgtgagcc gggcttacga cgaagccagc gggatcaaga atccgcgcat
     1741 ctggacggcc gagcgtgaca aggaaatgtg ggccgcgctg caagcctgat nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgaaaaactg ttcgccacgc atctggacgg
     1921 ccgagcgtga caaggaaatg tgggcggcgc tgcaacaagc ctgattccat tcaggcggcg
     1981 gtgtcaggcg tccaggtcaa ggcttgaaag cagggccagg aaggcggtga tggcgacctg
     2041 gccggtcagt gcttccagcc ggcccagtgc atcgtcatcg tccagcgtgg tgtagcccag
     2101 gctgaagcgc cggaacctgc gcacggcaag aggcccctgg tgcacgaccc gcaggttgat
     2161 gtgacgcgtg tcctgccgga tgcgcgccag ttgcgccaga acgtctttct ggctgccttc
     2221 catctgctcg caaaaacgca tgccgtcaaa gatgagcagg cccgtgatgc ccagccaggg
     2281 gttgtgcagg cgggctttcc cgacaatgtc cgcaatgacg ctggtgggcg cgtcgggggc
     2341 gagggtgctg acgtagatga cttcgtgcaa ctcggcggtg ggatcggtat gaggcatggg
     2401 tgcatgcaat atagcggtct gcagtggttg cggcaatgtg cctggccggc ggcggtttca
     2461 gctgtccata tccagggtcg ataacagtgc cagaaaatgt gccagcgctg cttcggcgcc
     2521 ggcgtcccgt agccgggcca gtacatccac gtcctcgaca gcggtgtaag cgaggccaaa
     2581 atgcctgaag cggcgctgtg cgagcgggcc gtggtgcagg atgtcgacat cgacatgccg
     2641 cggatcctgg cggatgcgtt cggtcagcgc cagcaccgcc tcgcggggcc cctcgatgtg
     2701 ctggcaaaac tgcatgccgt caaacaccag caggccggtg atgcctgcgg cctggttggc
     2761 cctgcgtgcc ttggaggcga tgtcggaaat gaccttgaga ggcgcgcccg gggcgagggt
     2821 gctgaggtac agcacttcat gcaattctgc tactttgttc atgggtggcg tgtaatgtag
     2881 ccagcgtgcc gcgacgcgac aacgtcaaaa gacacagggt ttcggtatta aaattttccg
     2941 gtgatcatca cgccgctctc cagttccagc ctgtgtcccc ggcgttgtgc agactgcggg
     3001 ttgcgcagga tgcaggcgtt ccagccgaac tccgcggagg aactggcttt tattgagtca
     3061 ttccgcagcg gcgcggcctt tgccccggcc ggcagcgcca tcatttcgga gcagaacccc
     3121 aacggcaagc tgttcaccct gtatgccggc tgggccttcc gctacaaaac cctgagtgac
     3181 gggcggcggc agattctcaa tttcctgctg cccggcgact tcatcggcct gcagcaggag
     3241 tttgccgacg gggcctcgca cggtgtggag gcgctgaccg acgccgccct gtgcgtgttt
     3301 ccgcgggacg gcctgtggga ccttttccgg caattcccca gcctggggta tgacgtgacc
     3361 tggctgtcgg cacgcgaaga aggcatggtc gatgaaggcc tgttgacgac gggccggcgc
     3421 aatgccacgg agcgggtggc gatgctgctg atccatctct accggcgggc cgagcagttg
     3481 gggctggtgc aggacagggc catggctttc cccatcaacc agcagcacat cgccgatgcg
     3541 ctgggcctgt cgctggtgca caccaacaag accttgcggc gcctgcacca gctggggctg
     3601 catgagctca agggcgggca gctgtctctg ctcaacccca aggcattgca ccggattgca
     3661 gactattacg acctgccgat gcgcaaagtg cccctgatct agtgtccttg taggccgggt
     3721 ctgacagcaa gcggatgggc ttgatgacag gtctcgccca cccgtctttt tacgctaaac
     3781 cccaaggtgt cgtcgatgtg ttgctgtgac acagggacgt tctgcttcag gagtttgcct
     3841 tgaatagcaa aagcatttcc ccgccacgcc gtctgcagca acagccgcag taccccgcga
     3901 accggcagca gcagcaaagc agtggttccg gtgaatttca gccacgtcgg ctcaggcagg
     3961 cgggtgagtc ctcttctgca gccttggcca acagcaaccc cccgcagcgg tgacggtcgc
     4021 tgccctgcca tcaaggatct atcatgacaa gcgaaaaatc catattgccc cggcccgaca
     4081 cgtccgcgaa gccagcgtca agggagcagc ggcaggtgcc gcccatcggc gaaggcagct
     4141 acgagggcac acgtgattac cagaaaagca tccagaccta cctggaagac gccgatgtgg
     4201 cggccgacgc acaggccgcg cgtcctgcgg acgccgccga agccgaagcg ctgaagcagg
     4261 ctgagcaaga aggcctgtcg cacgccaagt ccggggccaa taagggcgac gaagggcggc
     4321 ggccctgacg ggcgcctggg cgtcagcgcc cggcgaccag aatgccgaag tcctgcgcgt
     4381 agccctgcag ggtttgcacc gccctcgcgc gttgtgccgc cgtggtgctg ttgtgcaggg
     4441 ccgcaaaggt tttgcagctg tcgcgtgtca gccgctcttg gtagctgcgg taagcggggt
     4501 ccggcgaatt gacggctcgg tcaaagtagg cctgcatgag cggcttggct tgctcggacg
     4561 tggcgcggcc ggcaatcagc ggccccaggc tctgcaaggc gtcccgctgg cggcgctggt
     4621 attcggccag cgaaagccgc gcgtcaaaca ccgactgcga aatgcggctg cgcagcaccg
     4681 ccagttgcct gtcgtccagc gtaccgtaca gcatctcggc acggctgacc gctttcttct
     4741 gccgccggtt gatcaggtct tcggggctgc cctccagaaa atcgtcgcgg tactccgtat
     4801 tgagtttcga gaatttccgc ttcagggcct ccagctgctc cggccccagc ctacccacca
     4861 cgccgcccat ggcaggcgcg gcgcggtccg aaatggcttg caggcgggtt ttcacctcct
     4921 caaagatgcc gcaggcccgc gcctcgtcga tctcggcggg cagccaggcc ttccatttct
     4981 ccagtgtttc gacataggct ggcagctgtg tttgccggtg ccattgatgc aggcgtgcca
     5041 gttcttcccg gaccaggggc ttttgggtgt cgtcgaattc gaagtagctg ttgagctgcc
     5101 agtacgccag ctcggtcgcc tggttgtagg cgaatttgac cgcactgcag gccagcagtg
     5161 tcccgctcag cagcagcacc ccgataatct gggtgctgcg tttcagttcc ggcctggccg
     5221 gacgccactt tcgagacccc caattcacag gaaacttcat ggccactcca gttccagttg
     5281 atgtcgtcat catggccgcg ggcaaaggca cgcggatgaa aagcgcattg cccaaagtgt
     5341 tgcaccgatt gggcggccgt gccttgctgg cgcatgtgat tgactgcgca acgcggttat
     5401 cggcccggca ggcggtggtc atcaccggcc acggtgctgc cgaagtggag tccgcatgca
     5461 acagaatggc gcaggccgtc gccgccgtga atgccatgcc caaactgcag tttgcgcgcc
     5521 aggagcccca gctgggcacc ggccacgcgg tgcagcaggc gctgccactg ctggcggacg
     5581 acggtgtcac gctggtgctg tcgggcgacg tgcccctgac ccaggccgcc accctgcagg
     5641 ctctgctggc gcagtgcgac ggccagcgcc tggccctgct cacgctcagc atggctgacc
     5701 cgaccggtta cgggcgtatc gtccgtgccg gtgcagaacc ctccgcccag gtgcgcgcca
     5761 ttgtcgaaca caaggatgca acggatgccc agcgcgagat ccgcgaaatc tacagcggca
     5821 tcatggccgt gcccacgcgt ctgctgcgca gctggctggc ccggctggac aacaaaaacg
     5881 cgcagaacga gtattacctg accgacatcg tgaagtttgc cgttgccgat ggcgtggccg
     5941 tggtcgcgca ccagatcacg gacgctgcgc aggtcgccgg tgtcaacagc ccggtgcaac
     6001 tggcggaact cgagcgtgtt taccagctgc gccaggccac ggccctgatg gagcagggcg
     6061 tgcgcatcag cgacccggcg cggtttgacg tgcgtggctc gctggcctgc gggcaggacg
     6121 tggaaatcga tgtcaactgc gtgttcgaag gccgggtgag ccttggtgac gacgtccgca
     6181 ttggcgccaa ctgctgcatt gccaacgcca cgattgccgc cggcgcggtg atccatccct
     6241 tcacccacat cgacggtgaa aagctcggcg tgcaggtcgg cgcgggcgcg ctgattggcc
     6301 cgtttgcccg cctgcgcccc ggcgcgcagc tgggcgccga ggtgcacatc ggcaattttg
     6361 tcgaagtgaa aaactccacg ctggccaaag gcgccaaggc caaccacctg gcctacctcg
     6421 gtgacgccac ggtgggggaa cgcgtcaact acggcgccgg cagcatcacc gcgaactacg
     6481 acggcgccaa caaacaccgc accgtgatcg aagccgacgt gcacatcggc agcaactgcg
     6541 tgctggtggc ccccgtcacg ataggcgcag gcggcaccgt cggcggcggc tcgacgatca
     6601 ccaaggacac gccgcccggc gccctgagcg tggcgcgcgg caagcaggtc aacattgcca
     6661 actggcagcg gccggtcaaa aacaagcccg gcgcctaggc ggagcaggcc gatctggcgg
     6721 acgtggcgat ggctaccggc ccgcagggtc ctgtaagtat gctatttaat tcatagctgc
     6781 ttacgcacgt atctattggg ctagaagcag ttttggctca aacttcgagc gtttgatgct
     6841 gaaaaaggcc ttcacattgc gcacattgat gtcgctggtg aacaggcgct gcgcgagcgc
     6901 caggtaagcc ggcatgtcac gcacatgcac caccaggacg aagtccggac ccggcgagac
     6961 gcggtaacac tgctggacgg cgccgtcgag caccacccgg gcttcaaata cttcctgctg
     7021 ctcggcgccc tgccggtcca gggtgatttc caccagcgcc gtcaggccgt ggcccagggt
     7081 gccggccagc ttgtcggcgt ggaggatggc gacttcgcgt tcgatcaggc cggcgtcacg
     7141 cagccgcttg atacgccgca ggcaggtggg cggtgagata tgggcgcgtg cggccagggc
     7201 ctggttgctc tgcgaggcat cgtcctgcaa ggcttcaagc agtttgacgt caatcccgtc
     7261 gatggaaata ttcattttta ttccatatat ctaatgatta aggaatatta ttccataaat
     7321 gtattcttat gaaatttaat ttcattatta tttgaatttt gacgatcatg gaaaattaaa
     7381 aaggcatagc atccgcctat tgttttttca ggagcggttt atgtgcggca ttgtggcgtc
     7441 ggtttcgaca agaaatattg ttcccatcct tgtacagggc ctgcaaaggc tggagtaccg
     7501 gggatatgac tcctgcggtg tggcggtcta tgccgacggc ctgaagaggg cgcgcagcac
     7561 atcccgcgtg gccgagttga tggagcaggt gagcgctgac aagctcgaaa gcggcaccgg
     7621 cattgcccac acgcgctggg ccacgcatgg cgcgccagcc gtgcacaacg cccaccccca
     7681 cttcagccat ggcagcgggg acgctgcgca aaagccgggc cgggtggcgc tggtgcacaa
     7741 cggcatcatc gaaaaccacg acgaattgcg tgcagagctg caggccaggg gttatgtgtt
     7801 tgccagccag accgacaccg aagtgatcgt gcacctgatg gacagcctct acgacggtga
     7861 tctgttcgag gccctgcagg ccaccaccgc tcgcctgcat ggcgcctatg cgattgccgc
     7921 tttttgcaag gacgagccgc accgtgtggt gggcgcccgt tcgggctcgc cgctgatcct
     7981 gggggtcggc aaggacggca gcgagacctt tctggccagc gacgcgatgg ccctggccgg
     8041 cgtgaccgac cagatcctct acctggaaga aggcgacatg gtcgacatcc agctgggcaa
     8101 atactgggtc atcaaccgtg accacaaggc cgtccagcgc gaagtcaaaa ccgtgcaggc
     8161 gcacagcggc gcggccgagc tcggccctta ccgccactac atgcaaaagg aaattttcga
     8221 gcagccgcgc gccatcgccg acacgctcga aggcgtcgag ggcatcgttc ccgaactctt
     8281 cggcgacgac gcctaccgcg tattcaagga catcgactcc gtactcatcc tggcctgcgg
     8341 caccagctac tacagcggct gcgcggccaa gtactggctg gagtccatcg cccggatacc
     8401 gacgcaggtg gaagtggcca gcgaataccg ttaccgcacc tcggtgccca accccaggac
     8461 gctggtggtc accatcacgc aaagcggcga aaccgccgac acgctggccg cgttgcgcca
     8521 cgcgcaatca ttgggcatga cgcagacact gaccatctgc aatgtgtcca cctccgccat
     8581 ggtgcgcgag tgcaagctgg cctacatcac gcgcgccggc gtcgaaatcg gtgtggcatc
     8641 gacaaaagcc ttcacgaccc agttggcagg gctgttcctt ctcacgctgg cactggccca
     8701 gagcaaaggc cggttgagtg agcaggagga agagggccac ctcaaggcca tgcgccactt
     8761 gccggccgcc ctgcaagccg tgctggcgct cgagccgcag gtcatcagct gggccgaaga
     8821 ttttgcaaag atggaaaacg ccctgttcct ggggcgcggc ctgcattacc cgattgcgct
     8881 cgaaggtgcg ctcaagctca aggaaatcag ctacatccac gccgaggcct acccggccgg
     8941 tgaactcaag cacggcccgc tggcgctcgt caccagcgcc atgccggtgg tcaccgtcgc
     9001 gcccaacgac accttgctgg aaaaactcaa gagcaacatg caggaagtgc gcgcccgcgg
     9061 cggcgtgctc tacgtgctgg ccgacgccga caccaaaatc gagagcagcg acggcatcca
     9121 cgtcatccgc atgccggagc actacggcgc cctgagcccg ctgctgcatg tggtgccgct
     9181 gcagttgctg gcgtatcaca ccgccgtggc ccgcggcacg gacgtggaca aaccccgcaa
     9241 tctcgcaaag agcgtgactg tcgaatgaca gtcgcggtgc gcgcaggcgc actgcggggt
     9301 tttgggggag cgctcatgtc gcgtagcgat gtgatgtgat caccaccgcc gcacaatctt
     9361 gcgaagagcg tcaccgtaga atgatgggtt tggggcggca tgaaatgccg cccggcgcgg
     9421 ccgccaggtt tttggtttgg gggagctctc gtgtcgcgca gcgacgtgat gtgatcacca
     9481 caagccgcgc aatcttccca aaagcgtgac ggtggagtga acatgcccaa tacaccagcc
     9541 tgcccccagt gcggccagga aaatacctac cctgatggcg acaaccttgt gtgcgccgat
     9601 tgcggctacg aatggccgat ggtcgaaagc gcggctgccg acgacgacac aagcgccgtg
     9661 gtcagggaca gcaacggcaa ggtgctggcc gacggcgatg ccgtggtgct gatcaaggac
     9721 ctcaaggtca aggggtcgtc catcaccctc aagatgggca ccaaggtcaa gagcatccgc
     9781 ctggtcggcg gcgaccatga ggtggattgc aagatggacg ccggcagctt catgctcaag
     9841 gcgtgttacc tcaggaaggc ttgaggccac aggcctgacg ggctgcctgg ccgtttcgtt
     9901 cagtgatggt gcccgtgctc gccgtgcaca tggcggtggg caatttcttc ttcagtggcc
     9961 ttgcgcacgt cggtcaccgt caggctgaaa cgcagggcct tgccggccag cgggtggttg
    10021 ccgtccatca ggaccgtgtc gcccttgatt tttgtcacgg tgaaggcatg caccttgccg
    10081 tcgttgtcgc ggccttcgag ctggccgccg actttcacgc cgggcgggaa ctggcttttg
    10141 ggaatggtgc gcagcagcgc ctcatcacgc aggccaaagg cgtcttcggg ctgcagttcc
    10201 agcgtggttt ggtagccgcg cgtttttcct tccagcgctt cctcgatcct gggcaaggtg
    10261 ttgccataac ctccgtgcag gtaaaccatg ggctccttgc tttcttcaat cagcttgccc
    10321 ccgatttcgg agaccttgaa gcgcagggtg acggcggtgt ctttttcgat gttcatggcg
    10381 ggtcttggtg ggatgacaaa ccgccatttt cgcgcaaaga cggccgggct cggcttctca
    10441 gtgcagcagc ttgtcggctt cggacgccag cgcggcggaa atcgagtcca gcacggccga
    10501 ctccaggttc cagcagtgcc agaacagctg caccggcagc gtgtgctggg gcgcgacatt
    10561 gaccagctcg ccgtcgtcga tcaagccgcg tacgagaagc tccggcaaca cgctgacacc
    10621 ccatcctgcc agggcggcac gcagttgccc ctcggagctg ggaacaaaca gctggctcag
    10681 cgaaacccgc ttgaggccga cagcccgggc gacaaactcg ctttgcatgt cgtccttgcg
    10741 gttgaaagcc acataggtga gggcgcggaa attcgaagcg tccagcccgc cgggcagatg
    10801 gttttttgca taagccggct cggccacggc gacatagttc atggcaccga gctgggccac
    10861 cttgcagccg cgcagggcct gcttcagggt ggtgacgcaa cccagcacat ggccctggcg
    10921 cagccagtcg tgcgtgaaat cctggtcgtc gacgatgatc tcgaccggca gaccctgcct
    10981 gaccaggccg gtcagggcgt cgagcgccca gctggcaatg ctgtccgcat tgaccgcaat
    11041 cgagatgcgc tcttcctcgc gcacgccgcc cgacaagccc ggcgccagct ccttgaggtc
    11101 gcgttccagg tcggcgcgta tcaggcgcag ctgccgggtg tgtttgagca gcagcttgcc
    11161 ggccgaggtg gccttcagcg gccggctgcg cacgatcagg atggcgccga cctggccctc
    11221 cagcacccgc aggcgctgcg agatggccga ttgcgtgacg ttcaggcgct gggcggcgcg
    11281 ctcgaaaccg ccttcttcaa caatggcggc caggcattcg agggcaaggg gatcgtacat
    11341 aggcttatta gccttcctaa tgatttgcca aagagtttaa cgaggctgtt aatttgcggc
    11401 aagctcatcc agacttgggc ctgctcacac tatttatgca aggtgcgttg cgagtcaaaa
    11461 aagtcatgcg cgaggcgcaa aacgcagccg ggctgacgcc cggccaggct t
//
LOCUS       DAIDFG010000068        11448 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_68_length_11448_cov_17.459405, whole genome shotgun sequence.
ACCESSION   DAIDFG010000068 DAIDFG010000000
VERSION     DAIDFG010000068.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 11448)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11448)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11448
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68_length_11448_cov_17.459405"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2252 a         3737 c         3487 g         1972 t
ORIGIN      
        1 ttttcgaaag ccggtttggc cgcctgcaga taagccggat acccctcagg gttggtcacg
       61 gaaacccgaa caatccagta gccttttgac atgttttctc cttgaatgaa ggcgccccgg
      121 cagcgccgga atgaatgaac ttcgggaatc ttaggtttga ttttccatct ataaaaatga
      181 ataataaaga tgaagaacat cacaagaaga gatcatgaat ctaagcgatc tgcagatatt
      241 ccggacggtg gttgaagccg gtggcatcac gcgcgcagct gaacgcctgc accgcgtgca
      301 gtcaaacgtc accacgcgca ttcaccagct tgaagaaaag ctggaggttg aactgttcgt
      361 gcgccagggg aaaacactga ccctttcccc ggccgggcgt acgctgctgc cctacgctga
      421 ccggctgctg gcgctcgccg acgaggcgcg caacgccgta cgtgacggtg cgccccgcgg
      481 gccctttgtg ctgggtgcca tggaaagcac ggccgccgtt cgcctgcccg ccccactgag
      541 ccggtttgtt ggcgaccacc ccgacgtcaa gctcaccctc aaaaccggca acccgcagca
      601 gctcgcaacg gcgttgctgg caggccggct ggacgcagct ctggtgaccg ggcccttccc
      661 cgacggcccg ttcgaaagaa cgcctgtttt cagcgaagag ctggtcatcg ttgccgccgc
      721 cggacaagcg cctttgggtg gcaaaaacgg tcccctgccg ccagccatgg tggcgttcga
      781 aatggggtgc ccccatcgca cgagacttga aaaatggtat gcgcaaaaag gtggcgcacc
      841 cacccagacg attgaaattt cgtcctacca cgccatgctc ggctgcgtgg tggtgggtat
      901 gggtatttcg ctggtaccgc tcagcgtgct caccaccttt ccggaacgca agcacctcag
      961 cgtgcatccg cttcccaagg gcgacaacca ctacggcatt gacctgatct ggcgcaagga
     1021 agcggtggcc ccgcaaatga tccaggcgct ggtcaaagcg ctcaagccgg tcacggcaac
     1081 aaaatcgcca gcgcggcgcc ggctcagcgc tggcgcttga gcggctgcca gcctgcaaac
     1141 ttcgggtaat ggcgctgcac cacctggccg tcgaaatccc aggcgatgca cttgcccagg
     1201 tggtaaggcg gctcggcgtt ggcggactcc gcgaggccgg cctgcgcgcg cagatggccg
     1261 cggtacaccg gcaccgtctg gtaggccgcc gtcgccatgt tgaacagcat ctcgcgcagg
     1321 tgggtgcagc cgcgaatgcc gcccagcgcc ttttcgatgg cctggcgcca gcccggcccc
     1381 atggtggcgc ccaccatctg ctgcatcggg tcgatgccct gctggcattc gtcaaacggc
     1441 gcgtggtcac tggcgctggc gatgtcgcga atgaccagac cgtcgtccac cgtgacgcgt
     1501 atggccatgc cgtggatggg tgtgcccggc ggcacctcgc gctcggagcg gacatgttca
     1561 taggtacgca catcggtcat ttcggcttcg atgtcccaca gcccgtcctc gcgccggtag
     1621 ccgcggtaga cgacggcgcg tgtgtgcagg tgggttcggg cttggggtgc gggcagtggc
     1681 atgggcagaa agagttcggg caaaagagag cattttccca gcaacacggg cgccgtgccg
     1741 ccgggtgcgc cgcctgtggc agatcaaggc accaaatcgg ccctcagccc aattgggacg
     1801 ggcgcaagca gctcctgttt tgatagtacg ccaccaccag cggacccaga ccagaccggc
     1861 atactcgcgc cggggtcagt gctcattccg tcgaagacat cttgtagatg taggtaaacg
     1921 catgcccaaa tctcttgtcc aggctgtgga aaatctccat ggcgtgtgca ctgccgcaga
     1981 cgacgtaaac aagatcacgg ccatggttgg tgaacgcatc cagaatcatg tcggcgatat
     2041 agctgcttcg ggctttggcg ctgagtacat gcatgggctc aacccgggtg tcggcgaaag
     2101 ccggactcgg gatataagcg gcaacgttgt ccagaccccg ttcaaagatc actcgggtca
     2161 gaccggcttt catgacaggt ggattcaaca gtaccgcaga ggtgacggcc tggtccagcg
     2221 ggtcattgtg cctttcgccc agaaaaatga caccaaacct gcgcatcgga atcttgggaa
     2281 gcttctttcc caggaaagtc accgtgttgg cagcactctt cttcacactc gaaaaatcga
     2341 ccttcacgtc cttacctaaa ccgggcatgg cagctcctca aaatttgaac gggcacacag
     2401 cacccctggc ctttggccgc gcagcctccc aggcgacacg ccatggctcc atgcatcgta
     2461 gggagcttgc ggtcatgccg catcctgcaa aaggaggaga aggtccgtga cgccgggcct
     2521 gcaaaagtgc gatggacctg cgtcggcagc tgctgttttg acgatgctct gcagccggtg
     2581 cgcagggctt gaagctcccg cgctgctgcg ccagaatgga ccgcatgacc acctccgccc
     2641 cccttctcct gcaggacact ccgacgctgc gcatcggcga actcgcgcgc ttgagcggac
     2701 gcagcgtgta caccatccgt tggtacgagg cccagcacct catgcccggc gccgcgcgcg
     2761 acgcgggcgg gcggcgcgtg ttctcgcagg cgcatgtgga gtggctgggc ctgctgagcc
     2821 gcctgcgcgc ctcgggcatg agcgtacgcg acatgcaggc ctatgcgcgc ctggtgcgcc
     2881 agggcaaggc cacactggcc gactgccgcg agatgctgct gcagcaccgc ctcaaggtgc
     2941 agcagcgcgc cgaggaactg agccaggcca tggcgctggt ggaccacaag atcgcgctgt
     3001 acgacaagtg gctggcccgc gcggcaaccg gagacaggaa ggccagggcc ggttgacgcc
     3061 tcaggccggc gcgtccagcg cggcgtccac cagttgcgtg tccaggtact ccaccagttc
     3121 cctgagctgg ccgccttcga agcggcagac ataacagtag gtgttgttgt agggcttgcc
     3181 ttttttcgtg ttcacctggc cgcagcactc caccaccacc atgtcgccct cggcgatgaa
     3241 acgcgtggcc tggttggtgt aggtatcggc aaactgcgca aacagcgggc gaagcagctc
     3301 gtcccgcacc gcctgcttgc cctcgtagcg ccgcgaccac gccgtccggc ccggcaggat
     3361 ccagcagaag tcatcggcca tggcgtccac aaatggccgg ccattgccct gcgcgagttc
     3421 agcaaagatg gcctgcatca gttgcttgtt ggtttgggat tcggtggtca tgtcaggggc
     3481 cttttcatgc aaaaagttga agaggtcctg attggaaaag ctcgagtgca ctcgaagtca
     3541 agcgcaccct cgcgcccagc taccggacgg ccgtcatggc ggcccccgga tcaagtccgg
     3601 ggcaggccct ggtacgccgt ccatgggggc atcaccgaaa cgctggtcaa tgcagcatgg
     3661 atcccggatc ggcgtccggg atgacaaacc agggttcagg atgacaaatc aggctgcagc
     3721 ccttctgcgc agtgcgcagc cagctctgtt ttcaatagca gacagcgccc agccggcgca
     3781 tcaatcccag acgagattgt tgcaagcctc gcactcatcc ttgagaacgc ccatccggac
     3841 cagcagcgcg tggaccttgc aacccacgca aacgcccagg acggcttcca tccacatgaa
     3901 gcccaggcag acccagacca gcaccagcgg aatccagcgc ggcatgaagt tggacgtcgt
     3961 ggtcaggagg gcgtggccgg tcagcccatt gacccagccg gcaaaaacat ccggattgaa
     4021 gaagaccagg caggtcgcga tcatcgtggc accgatcgac caggcaaagc gtttgggcag
     4081 caaaggcttc cagacgggct ggctgccttt ggccaacagg ctggcgatga aaatcgtggg
     4141 ggacagccgc gagctccata cgaacatgcc cgcgatcatt tcaaacaggg cataaaacag
     4201 cacataggtc tgtttcgagt attcaaaggt acggcggacc gcttcgacgt tgtagagaat
     4261 gcgcccgtca aaatcggtgt cgaaggtgtc cttgatgacg ttgcccgtga ccacccagtg
     4321 tgatgagaac aacacgtcat aaagcgtcag gcccatgtag agcgggatca gcagcatcag
     4381 tccggcacgt attcgcaccg ccgtgtcgtt gatgaagaca cttttttcgc ccgggtcgcg
     4441 gtaccaaagc ttttgaaaga cgtttcccaa actctttaac gcattcatca tgatcatgag
     4501 ccttccatgg gtatttataa ccgacatctt atatagattt ctgtccattt caagcatggc
     4561 aaacccggag ccgcagacgc cagcgcagcc gctggtttgt ctgccacaat cccacaaggt
     4621 acacgccaca tgcccgtgcg gtacccggga atcgcaacgc tgttactcgg ccgctacacg
     4681 cgcaaaggtg atgatcatgg caagaatgtc gatgtttgca ctgaaacctg catgggacgc
     4741 gatgacggcg aaacgccgca gcctcctcta cggcgccgcc ttcgcgccgg ccctgttgct
     4801 cgccggatgc gccgtggaag tggagaacac gcaggctgcc caggaggtcg cacggctctc
     4861 caggccgccc ggttcggtcg ccatcgggtg gcgcgtcttc cagaacaagt gcgcaagctg
     4921 tcatggtgcc gctgcgacag gaacggcgaa cgggcccgac ctgttgccca gggttcggga
     4981 tatgggctca cgccagttcg tgagcctcgt actcaagcgc tacgactgga atctccctgc
     5041 ggcgcaagcc ggcagcgagg gcgccgcgcg ggaagccctg atcgacgata tcctggagcg
     5101 caggaggaac ttcaagccca ccatgcccgc gtgggatggc gaacccatcg tgaccaccca
     5161 tatcgtggat atccatgcct atctttcggc gcgcgcgcaa ggcacgcagg gcccgggtcg
     5221 tccgctaccc tagcagcctg tctgtcattg cgggccccgg atcaagtccg gagcaggctc
     5281 tgacccgcaa tccatggagg cataaccagg actgcggtca acgaggcatg gatcccgggt
     5341 cgagcccggg atgacaagcc agtgcccggg atgacaagcc agggtccgag atgacaacga
     5401 cgcgctgcgc cccctgtctg tcattgcggg cctgacccgc aatccatgtc ggcgtacccc
     5461 ggactgtggt caaccaggca tggatcccgg gtccagcccg ggatgacaag tcagggttac
     5521 ctatgtttcc accaccgaat gccaccagat cctgaagtcg cggcacaggc cgccggcttc
     5581 aaactcggcg accgccacgc cgtccagcgc caggcgcggc aaggggtcgc ccggctggcg
     5641 cgcgagctgg ctggtgccgg tggaggcagc ccacatcgca aacatttctt ccgaggtgac
     5701 ctggtaggtg gtgtgccagt gcgcaatacc gcccagcgcc tcggcatgca ggatgtcgaa
     5761 ggtgagcacg atgtcggtct gcagcttgac gcgctgccag tactgcttga tctgcgtgtg
     5821 cccggtctgc accgcaaacg gcgtgttgtg gtactggccg tcttcggtga acagcgaggc
     5881 gagaagaaat tcgtcgcggg tttcccacgc ctttttgtag ttttccatga agcgctgcat
     5941 cagcatctgg gggcctccgg tggttgatat tctggtcact cgtaacgcac tccgtcgcgg
     6001 cgaaacgtta cgtagcagcc aggccaaagg atagttcatc gaagaagaac ttccaggccc
     6061 gcccaatcat gtccacccac gccaggaatg aagccatcct gaagttcgca ctgggccggg
     6121 tgggcgcagg ggtgcaacag gtggtgcggc atgcaagtcg atctgccgcc tgaccgcctt
     6181 ttgagtcaaa tcatgctcca gcccttattc cacgggcgca agcagctacg ttttttatag
     6241 cgtaaccctt ccacacgccg ccttgcccgg tcacgcacga aggccgcagc ggtcaggcac
     6301 ccgcgactct ggcctacacc cctgccgccc tgccggcact aggctgaagg cacaaaccct
     6361 caggagttag tcatgaccca acccggcgta gaacccgaac gcatcaccat gaacaacctg
     6421 gacaactgtg cgcagtgggc caaaaagctc aacgtgaccg acgaacagct gcgcgaagcg
     6481 attcaggtcg tgggcgacca ggccacagac gttgaaatgc atctcaaggg cacgcgcagc
     6541 accaccaacg acgagcgtat gcatgatctc cgcaatgcat gactgccccc ccactccgcc
     6601 cacgcttttg atgccgacac ccgcctgacg ggatggaatc tttccttggc agcgcgctgc
     6661 acggcgcggc ggcggttttt ctgggtctgg ctgccttgct ggggctggtc atctggtcca
     6721 tcaagcggca caacagcccg aaactgaaga tcgaatcctg cgagtccatc gacgagctga
     6781 ttccctcact ggcgggcctg accctgagct cggccgtggc cggcaactcg gtggcgctgc
     6841 ttgaaaacgg cgccttcttc gacgtgctga tcgagcgcat aggcgccgcg cagcaaaacg
     6901 tgcactttga aacctttctc tggaaagacg gcgtgctggg ccagcgcatg gccgacgcgc
     6961 tgtccgagcg ggccagggcc ggcgtgaaag tgcgcgtgct gctggacgcc accggctcca
     7021 aaggcgtcgg caaggcaacc cgccggcaga tgaaagatgc cggctgcaag ctggtgttct
     7081 ttcatgaaaa ggcgcttcgc aacattggcg tattgaacga gcgcgaccac cgcaagctgg
     7141 cggtgattga cgggcgcgaa gcctttgtgg gcggccactg cgtggtggac gcatggctgg
     7201 gcaacgccga agacggccaa cactatgccg acctcagcgt gcacctgcac ggccccatcg
     7261 tgcatagcgt gcaggccgcc ttcagcgaaa actggggcgg gcaaaccggc gagctgtttt
     7321 tgggcaagga cgtgtttccc gagctggcgc ccgccggtaa cgtgaccatt cacgcggcct
     7381 acgccaagcc ggagggttcg gcccccgccg tcaagattct tcaccacacc gtgatctgcc
     7441 tggcacaaaa gcgcatctgg atccagaacc cctacttcat ccccgaaccc gaggcgattg
     7501 atgcgttcgg cgccgccgtc aagcgcggcg tggatgtgcg ggtgatgatg ccgtccacca
     7561 gcggctcgga caaccccatg gtgcaacacg ccggccaccg caactttgaa aaactgctca
     7621 agtgcggcgt gcgcctgttc gagtacccgc acactctgct gcaccagaag gtcatgacgg
     7681 tggacggcgt gtggagcgcc atcggctcca gcaacttcga cgaccgctct tttgacacca
     7741 acgacgagct caccctgggc atcaaggacg ccgccctgac gcagcaactg gacgcggtgt
     7801 ttgaaaagta cgtgcagcgc tgcgaagaga tagacctcaa gcaatggcaa cagcgcgggc
     7861 tgtggcacaa gttcaaggac aacatgttct acgtgttcaa cgaaatcctt tagcacggac
     7921 ctttgcggag tgatcgggtc ttgtggcaga ttcttatagt caaatttgcc actagccctt
     7981 tatccacggg cgccaccagc tacattttat atagcaaagg cacatccttt ccgcgctgcc
     8041 cgcagagcgt cacggcggtt cagggccatg cagcacaatg cagggcatcc accaaaccgg
     8101 gctccgcagc cccgcctgaa catgcccagg gacatccaca gtgaagccat cctgcagttc
     8161 gccttgcaag ccaagtgggc gcgatggctg gccgcgcatc atggccaaac ggagggggtg
     8221 tggttacgcc acgccaaaaa gggcgcaccg accgccaccg tcagctacac cgaggcactg
     8281 gaagtcgccc tgtgtttcgg ctggatcgac agccagaaaa aagccgagag cgaacatttc
     8341 ttcattcagc gctggagccc gcgcggcgcg cgcagcatct ggtccaaggt caaccgcgac
     8401 aaggcgctgg gctacatcca gaccggctac atgcagccgg cgggcctggc ggaaattgaa
     8461 cgtgcgcggc gcgacgggcg ctgggacgcg gcttacgacc ctgccagccg gtcagagatc
     8521 ccggccgacc tgcaggcggc gctggatgcg ctgcccggca ctgcggagtt ttttgccacc
     8581 gtgagcgcgc agaaccgcta tgccgtgctg ttccgcctgc agaccgcgaa gaagcccgaa
     8641 acgcgcgccc ggcgggtggc ccagtttgcg cagatgctgg cccgcggcga aaccttttac
     8701 ccgcacaacc cgcataaccc gaataacccg aataacccgc gcaagacaaa agcctgaggg
     8761 ccgtcagcgc cccggcgtct tgcgctgctg gcgcgccaca taccagcgcg ccatgcgctc
     8821 catcagcacg tccaccctgc ccttgagccc gggcgccacc ggcggcgcgg cgtgcaggtc
     8881 gatctgctgc ccgcccgcct tgtacatctg cacaaacatg tcgagcacct tctggcgcac
     8941 catgccgcgc aggggaatgt ggctcatcat gccgtaggtg gcggcgctgc cctcctgcgc
     9001 cagcgcgggg ttggccctga cggtggccac ggcgtccttc aggtcgcgca aataagcatc
     9061 caccacggcc aggtggccgg cggtgatcat ggcgtgcagg ccgtcgggtt tctgcaggcg
     9121 gttgaccgtc cagcccttcg catccatctg gtcgcccacc gcaaagatgt tgaccgccgc
     9181 atcacgcgag ccatacgcca gcagcggtcc gctgggttcg cccatcacat acagctcggg
     9241 catggcttcg acgccttcac gcatggtgtc aaaggcctgc agggtctgcg cggccagcgc
     9301 gcggtagccc gactggccga agtgctgcat ggccgcccag gccgccgcat aggcgccgcc
     9361 cggccgcgtg cccagcagcg ccggcgaggc aaacacgccg ccgggccagt cttcatacac
     9421 aaacatctgg tggcgcaggt agtccaggtt gcggtaggtg atggtggagg cgcccttggc
     9481 cgcaaagccg tatttgtgaa tgtcggccga gatcgaggtg acgccgggca cccggtagtc
     9541 ccacagcggc agcgtgcgcc cgttcatggc catgaacggc aggatgaagc cgcccacaca
     9601 ggcgtccaca tgcaggggaa tgccgcgctt gagtgcgatg gcgcccatgg ccgctatcgg
     9661 gtcgatgctg ccgtgcgggt actcgggcgc cgagccaaga atcatcaccg tgcggcggtt
     9721 cagcaggccg ggcagcttgc gcacgtcggc gtgcaattgg tcgtccagcg gcagcaggcg
     9781 caccttcaca ttgaagtatt ccgccgcctt gaaccaggcc acatgcgccg tgacgggcag
     9841 caccatctcg ggccgcgtca cgccgcgctg gctgcgtgcc atgtcgcggt aggtcttgac
     9901 ggccagcagg cagctttcgg tgccgccgga ggtgaccacg ccgcacacct ccggcgtgcc
     9961 gtggaacagc tcggccacgg ccgcgatgat ttcggtctcg aaacgcttga ggcttttgaa
    10021 tgccgtgggg ttgagcccgt tggccgacga aaaggcctgg taagcctcgc ccatgaaggc
    10081 gttgtgttct tcgcccaggt aatacaccag gctccacagc cggttgccct tgtagtcggg
    10141 gtcctgcgcg ccgtagccct tgagctcggc cagcacctcg gcctgggggc ggccctgttc
    10201 aggcagcttg cctctgcccg cgctgtctct gtcatcccgg acttgaggag cctgccccgg
    10261 actccgatcc ggggggatcc atgtctgctt accctcgccg ctcatggctg ccccgcctct
    10321 ttgccgtcaa tcttgacttt ggcacgacgc cgcgtcagca gccagacgcc gatcaccagc
    10381 aagatgaaca ccggcagcaa cacgccctga aagcgctgca tcagcgccag aatctcctgc
    10441 cagcgcccgc ccacataacg cacgccgtac accaacaggg gcagctccac cagcgccgcc
    10501 gcgccgtcgt acacaaagag gtggcggtac ggcatcttga gcgaacccgc cgcaaaaaac
    10561 agcggcgtgc gcacgcccgg catgaaacgc accacaaaga tgtaggccgg cccgtagcgg
    10621 cgcatggtgt cctgcatggc ggccagccgc gcctcgggta ccacgcgcgc aaagtagcgg
    10681 tgccgcagca gccgcgcccc gaagcggtgg ccccagtgaa acaccagcgc atcgcccgcc
    10741 accagcccga accacgccac caccatcagc ggcaccggct ccatcacccc cttgagcgtg
    10801 agcgccgccg ccgccagcag caggatgtct tcgttgatgg gtgcgcctat gccgcagctg
    10861 aacagcagcg cgaacagcag cgcataagcg ggaaagccct gcagcgactg caggaaggcc
    10921 atggcggaat caaacatggg cgctattctg atgcctcgga aaaaggagga acagctggtt
    10981 tcccccactg caggccatcg accggacccg actgccaggc cacttccacc ccatgcggca
    11041 gcgcgctaca gttgcttgat gcacctcaag cccgtcagcc caagcccggc gcagcataac
    11101 cacccccact gaaggaacca ccatgactga caacgctctt gccacccttc caccaccccg
    11161 caagctctgg gtgcgcgcgc tgctcatgtt gctgatgtgc gcagccttcc agctggctgc
    11221 ctgggtgctg gtggccgtcg ccctgctgca gctgggcttt gccctcttca ccgaccagcc
    11281 caacccgcgc ctgcaaggct tcggccgcag cgtgggccgc tacctggcgc agatcgcggg
    11341 cttcgtctcc ttcgccaccg agaccctgcc cttccccttc accgactggc cggccgaaga
    11401 cttgtgagca gcatgcaggc atagcccttc accaaggatt gtcatgag
//
LOCUS       DAIDFG010000069        11420 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_69_length_11420_cov_17.759613, whole genome shotgun sequence.
ACCESSION   DAIDFG010000069 DAIDFG010000000
VERSION     DAIDFG010000069.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 11420)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11420)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11420
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69_length_11420_cov_17.759613"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2175 a         3709 c         3585 g         1951 t
ORIGIN      
        1 acaccgtctt ccacgatgat gttgcaggct gtgacggtgg tgggcgcgtc cggcaggccc
       61 accagcgcgt tggccccgat caggatgtcg ctttcggaca ccaccggccc ccaggcccgg
      121 gcattcttgt aaacgtacac gcgcttttcg caggtcacgg cgccctgcac gctggcgcgg
      181 tggccgatgc tcactccttc gcgcgccttg atgtcgccga tgagggtggt cgccgcccca
      241 atggtcagaa aaccggtcac gaccagcgat ccgcggtaaa tgctggcagg cggcagcgcg
      301 caatcccccc ggatcaggaa aagcaggggc gtttgctgga cggcgcccgg caggtcggcg
      361 taactggccg gcgcctgctc ggcgccggca ggcggcagca catcgctgac gcgcgagcca
      421 aaacgcaggg caggtgcatg cagacgctca aaccaggtct cgttgccgag ctcgatggca
      481 ctgcccgcgg acacccgtct gagggccaca ctgcgcggcc ccatgcgcag cacgccgtcg
      541 gcatgggccc agtcatcgac ggtgctgtgt tcacccaggt caatgtcacc ggtggcatac
      601 agcgccggaa aggccgattc ggcgccagcc tgcaagcttc cctgcacata aaccggctgt
      661 gcactgcgaa tgggcaaggg gctggttatg ctgctgcgcg cgatcagccg cttgtctgcc
      721 ttgcgccagt ccaggtccgt gccggccgcc gacgccggat cgccgacaaa ttcgaagttc
      781 tcatacccgg tgggctcgcc cagcccgagc ctggccgaca catcggcatg cagtcgccgg
      841 gcaaagtgat caatgtcggt ggtgtagttc gccgaaatcg gcaaggatgc aaagtcggtg
      901 ggacgcagcc attcccggaa tgccggcaca aacggcagcg tcagcagcag caggcagacc
      961 agcgcgaagg tggcataggt ggtgtagttt tcagtcatta ttcagcagcc ttggtgcggt
     1021 agcggatggt cttgtcccag accatctcgc gtttgaaaat cctgtcgagc agcaggctcc
     1081 agacagcgcc agaaatggca aacaggctga ccagaaagcc cagcatgttg aaaggcagca
     1141 gcctcagccg ccgccggttg ccgtcaatca gcaccgcgac cacaatttca aaaaatgcgg
     1201 cgaagttgcc cagcgttcca taaatcatca gtgcaaaaat gggaatcagt gacgacagca
     1261 gggaacccgc attgaggaaa tacagtccca gcgccaggca ccagccgagc agcatgacca
     1321 gcgggatgac aaacacaaac agcagcaaca ggccgtcaaa acgctggccc ggtgtcaggt
     1381 aggcgctggt ggcgaacttc caccaatacc gcgtcatgac ctggttgtgg cccttggccc
     1441 agcgcgtgac ctgcttgatg cgcacgctcc actcttcggg cacttcttca taacattccg
     1501 agcggttggt gtacaccgtc ttccagccgt tgagcatgag ccggaacgtg atgtcagtgt
     1561 cttcggccag cgtatcgtcg tgccagccac ccaccgcctc gactgccgac aggcgcacgc
     1621 caccgaccgt gccgccgtac tggggcagca ggttcaggtt catgcgcgcc tgctgatcca
     1681 cctggtagcc gccggatcgc tccaggtcca gcatgcgggt cagcaggttg gcgccgctgt
     1741 tgacgggcac cacacgtccc atcacagcgc cgatctccgg gtcgaaaaac ggcgccacca
     1801 gctgcttgag caggccgcgg ccgggcacgt agtcggcgtc aaaaataatg gcgatgtcgc
     1861 cttcggcaaa gtgcagggcg tccttcaggg cggccgactt gccggccttg ccggtgctgc
     1921 ggtgaaacgg cgaaatgcgc gaggggaaac gcgccacata actgtcgatg atctcgcccg
     1981 tgccgtcctt cgaccggtca ttgacgggaa tgatcttgag ccggtctgcc ggatagtcgg
     2041 tattgagaag cgcctcgata cagccagcga tgactttttc ctcgttgtgg gccgcaatga
     2101 agacggtgat catgggccag cgcgccacgg caatgtcgat gtaggggtgg cgctgcacac
     2161 cggtcagccg gttcatcgtg aacatgaagt ggcgcacgcc gtaaatcatc attgccacca
     2221 cgatcaggaa aagcagcgag gccatgacgg cggccaccgg gtagtccttg aacgagggaa
     2281 tcagcgcacc ggtcgcctgg gcttgcgcca gggccggccc ggcccccatg aaggaagtga
     2341 ccgcgagggc gagcgcgaca cggggccggc ccggccgccg ggtggcgtga acgagccctt
     2401 cggctgaaag gccgcaagag gaaaatgaac aagaggaagg cagcatgggc gtgactccag
     2461 taagaagggg atcggtcagg ccagcgcggg gatggccgcg ggcgcatgca ccaggctgcg
     2521 ctcgagcaca gccacgatct gcgcggaggc cgtgccgtcg ccgaagggat tgccggcctg
     2581 cgccatccgt gtgtaggcct gggcatcctg cgaaagccgg gtgacttcgg cgacgatcgc
     2641 gctgcgagtc gcgccgatca gcagggcgca gcccgcttca atcgcctcgg gccgttcggt
     2701 ctcgtcgcgc agcaccagca ccggcacgcc aaaggtcggg gcttcttcct gcaggccgcc
     2761 ggaatcggtc agcaccatcc acgcatcggc cagcgcctgc tgcatgccgg tgtagtccag
     2821 cggcgccgtc aatgtcacgt tggcgatgtc gcccagcagt gcgttgacag gaccctggat
     2881 cacgggattg aggtgaaccg gaaacagcac cttcaggtcc ggctgctggc gagcaatgtc
     2941 cgcaatggcg cggcagattt cctccacgtc gttaccccag ttttcacgcc ggtgtacggt
     3001 caccagcaaa tggccgcggc ccgcgctcgt gcgcttgacg ccgcggtggg cgcaggtcca
     3061 ttgctgggca tcaatcacgg tattgcccgt gagatgaatg aactcgtcgg caatgccttc
     3121 ggtctgcagg gccaccatgg cgcgctgcgt gggcgcaaag tgaaaccgcg tcaggcggct
     3181 gatcatctgc cgcaggccct cctcgggaaa cggccgggcc aggttgtagg tgcgcagacc
     3241 ggcttccaca tgggccagcg gtacgcggtt gtagaaggcg gccagcgcgc cctggaaggc
     3301 actttcggtg tcaccctgca cgatgacaag cgaccagtcc tgcgcggtca ttaccgcgtc
     3361 gagctgctgc cggcacccca tgctgaaatc cagcaggctg ctgccgccgc gctccagcga
     3421 gatgtcgggc gtcacatcga aacattcgaa gatctggctc gccatgtcgc catgctgtcc
     3481 cgtgtgcagc cattgcacac gcgcccagtc ggtcgcacgc agcgcgtggt aaaccggcgc
     3541 cagcttgatg atttcagggc gcgtgcccga gacgatgaga atgttttgca tggatgtttt
     3601 cctgaaaaag gaggagaacc gacggcgacc tcgcacacgc aggtgaaggt gcagggggcc
     3661 gaaggcatga gtcaagccgg agtgcgcggg cggttcaccg ccccgggtca ggccagttga
     3721 atgtgagcgc cgtggcccgt catggcggcg ggagtcaaca agtcaacaat cacgaccagg
     3781 tcgccgccga aacgccccac gccgagttcg cgcatgagtt gctgctcctg cgcggccgag
     3841 tccatgcgca aaaagcacag ggcttgtcgc ttgccggcct gactggcggc gaggatgtcc
     3901 agccgggcga cggagccata acgcgaacac agcgagtgaa gtgccggtcg cagtgcgctg
     3961 atgtcaggga agttctttag ctgatcgaat tcgttgtgca taggtattgg agtcctgagt
     4021 tctttttccc tctggaatgg gtgttaactt aagttctcag tcaatataaa aaacccgaag
     4081 cgccgggcac ctgagggaat gccgtccctg gctgtaggac gctgcgccgg tccttctgtg
     4141 cgctggcgca cacaggcaca gccaaacacc cccgcttttg ccacccggca gcgggccacc
     4201 gaggcggcca gcgcacgctg gctgcaccgt cctacaaccc cgcgcgccga cggacggctt
     4261 tttttttaat ttcccggcgc ctcatggaag aagccgcttc attccgccgc ccggcaagca
     4321 gttcaactcc tcacaggaca accaccatgg cactcatcac ctacctcgcc gaagacaaca
     4381 agatcattcg ggacaacctg attgacaccc tggaggagat cgcccatgtg aaggtcgtgg
     4441 cgcacgcggt cacccaggcc gaagccagcc actggctgac gctgcatgac ggcacctggc
     4501 acctggccat cgtggatctt ttcctcaagg aaggcagcgg gctgggcgtg ctggcggggt
     4561 gccgcaaccg cgagccctac cagaaggtgg tggtgctgac caactacgcc acacctgaaa
     4621 tccgccggcg tgcggccgaa cttggcgccg acgcggtgtt tgacaagacc accgagcttg
     4681 acgcgctgtt ggcttactgc gccgaacaaa cggcccaggc ccacacggag gatgtgcagg
     4741 aggcgcagcg tgaagccgtc gcgcagcacg atcaggccac gcccacctga ggaaacctgc
     4801 gttcgctccg cccaacaggc cgcctgtcgg tttgtgcgct agcgtacata caaagcatcc
     4861 ggaacggcct aatatggcag actgactcga aacctctgcc agaggcgccc ctcgaagcca
     4921 gataccgctt cggagctttg ttgatggccg tttcccccga tccccgaaaa aacgagttgc
     4981 ttgcagcctt gcctgacgcc gagtttcagc gctggttgcc gcaactcgag tgggtcgaca
     5041 tgccgctggg ccaggtggtt tatgaatcgg gcagcaccct gagccatgtg tacttcccga
     5101 ccacctccat cgtctccctg ctgtatgtga tggaaaacgg cgcctcggcc gaaatcgccg
     5161 tggtcggcca cgaaggcgtg gtcggcattt cgctgttcat gggcggcgaa tcgacgccca
     5221 gccggggcgt ggtgcaaagc gccggccagg gcttccggct cccggcgcac gtcatcaagg
     5281 aagaattcaa gcgtgcgccc gtgttgcacc tgctgctgcg ctatacccag gcgctgatca
     5341 cgcagatggc gcaaacggcg gtgtgcaacc gccaccactc gctggaccag cagctgtgcc
     5401 gttggctgct gctgagcctg gaccggctgc agggcaatga gctggtgatg acgcaggagc
     5461 tgattgccaa catgctcggc gtgcgccgcg aaggcgtaac cgagagtgca ctcaagctgc
     5521 aaaacctcgg cctgatccgt tattcgcgcg gccgcatcac ggtgctggac cggcccggcc
     5581 tggaaaaccg cacctgcgag tgttatgccg tggtcaagaa ggaatacgac cgtctgctcc
     5641 ccgacaagct ggcgatctga cccctgtccc gtcggctcgc attgcccctg attcaaagac
     5701 agatagatta tctagcgcat actgggccac cgactggaac tggatctgac agtggcgcgg
     5761 ccttgtaatt gcccccgtga cggggccgca acccaccgga gagccgcttg cccgcagtcg
     5821 aaaaccacct cattgaacgc cttccccgtc aggaccgcat ccgcctgctg gctgcttgcg
     5881 aacccgtcga actggcgctg tccgaagtcc tgtgcgagcc cgaccagccg gtgcgccacg
     5941 tgtactttcc cgtccagggc tttatctcgc tggtcacgcc ggtcgacggc agtccgggcg
     6001 tggaggtggg catgataggc cgcgaaggca tgctgggtgc gcagctggcg ctgggtgtcg
     6061 ccacagcgcc ctggcgcgcg ctggtgcagg gcgccggtgg cgcctggcgc atccgcacgg
     6121 ccgcgttcaa gcgcgagctg gcgcgcagcc cggcgctgca gcgcggcctc aaccgctacc
     6181 tgtacgtgct gatggcgcag cacgccgcgt ctgccggttg cctgcgtttt cacctgaccg
     6241 gccagcgcct ggcgcgctgg ctgctgatga gccaggaccg cgcccactcc gacagctttc
     6301 aagtcacgca cgagttcctc gcctacatgc tgggcatgcg ccgcgtcggc atcacggccg
     6361 ccgccagcgc cctgcaaaag gaaggcctga tcgagtacca ccgcggcaaa ctcaccgtgc
     6421 tggaccgcca agggctggaa gccgcgacct gctgttgtta cgccgccgac cgccggacct
     6481 acgccgactt gctcggctag ccgcccgcta ttcgcttgcc cgttcgccct gagccggtcg
     6541 aaggggcgaa cggttgcaac cggcgcgtcc gtacggtagc gcaccgacga tagcaaaaga
     6601 atcatttaca acaagcagta tcggaccaca aactacttgt agctgcagcc tcacatgcaa
     6661 tcacggcaac cgccccttgg cacacagggc acatgacatc gagaggctaa aaaattgacc
     6721 tcgtcctcct ttcctccgcg tacgccaagg cagaccgctg gcatgcagcc cgacatggac
     6781 tccccaccac cagcccaggt gtcgggccag aacgggctcg gaatcccccc tgaggcagcc
     6841 gccccccatg caccgtatga cgcgcacctg gccattgagg tcgaggactg ggatgtcctg
     6901 ttcgaggcgg taaaaaccac gctgcggcgc attgtgggtg agcggctggg agacatgccc
     6961 gaggtgcccc cccattcggc tgcgctgtcg gccagcctgg tgcaggccgt cgtgcttgat
     7021 tgcgtcagct cgctggaccg tctgcacgcc gcgctgcaac aggaacgcag ccagcgtccg
     7081 acaccctgac gggcactttg cgaagcccgg gccgacactg cgtctgttag cgtggtcagc
     7141 atgccggtca cacgccccct tcccccgccc cagaacctgc ccccccgccc cgcgccggac
     7201 gccgccccgg acgccgtccc gccgatgcgg gccaccggtg cgggcgcgtg gatgctggcg
     7261 ctgccgtcgc accagctgac atggacggcg cacctggctg ccatgctgga aatgacatcg
     7321 gagcaggcac cgtcatgggc caacgccctg gatatgtatg cgcccgagtc gcgcgcctgc
     7381 atgaccgcgg ccctggagcg ctgcctcgca gacggcacgg cgtttgacga agaggtgcag
     7441 gtcatcaccg cgcgcggcca gcggctgtgg atgcgttcgc tgggcgaggc cctgcgcgat
     7501 gcatcgggcg ccatcgtgca attgatcggc gttctgaaag acctcacgac ccgaaaacgt
     7561 gccgagcagg aaagcgaaag cctgaccatg cggctggcga ccacgctggc gagcatcacc
     7621 gacgctttcg ccacgctgga ccgcgagggc cggctgactt acgtcaaccg cgagagcgaa
     7681 cgcctgctgc gtgctccggt cagggcgctg ctgggtcgca cgctctggga cgtgctggac
     7741 ggcaacggca caggcccggt gcgccaggag atcctggcca tgcttggcgg cggcgccagc
     7801 cgcgagttcg aggttttctg tgccattccc gaacgctggc tggagctgcg cgcctaccct
     7861 tttgaagagg gcgtggccat gtacctgcgc gatgtcagcg acaagcgcca gtcgcaggag
     7921 cagttgctgc tgctgcaaac ctgcatatcg cacctgaacg acgtcgtgat gatcaccgaa
     7981 gccggcccgg tggacgcgcc cggcccgcgc atcgtgtttg tgaacgatgc cttcgagcgg
     8041 cagaccggct acagccccaa cgaagtgctg ggacagacgc cgcgcattct gcaggggcag
     8101 cgcacgcagc gcgtcgagct ggaccgcatc cggcaggcgc tgcagcgggc ccagccggtg
     8161 cgcgcggaac tcatcaacta caaaaaaaac ggcgagcagt tctggctgga actcgacatc
     8221 gtgccggtgg actatttcag ccgggggctg acgcactggg tggcggtggc tcgcgacatc
     8281 acccagcgca aggccgccga ggaagaaatc gagcacctgg cgttttacga cacgctgacc
     8341 cagctgccca accgccagct gctgatggaa agcctgcgca ctgcactgac tcacacggac
     8401 aaccccgaca aaaccggcgc gctgatgttc attgaccttg acaacttcaa gatgctcaac
     8461 gacacccggg gccatgccac cggcgacctg ctgctgcaaa aggtggcggc gcgactgaac
     8521 agctgcgtgc ggctgcgcga caccgtggcc cgcctgggcg gcgatgaatt tgtggtcctg
     8581 ctcgaagacc tgggtgacga cccgacgctg gcccgccaga aggcaaaagt cgtggcatac
     8641 aaaattctgg ccaccctgtg cgagccctgc gacctgtccg gccacgacta tgacgggacc
     8701 tgcagcatcg gcatcaccct gatcacgcag cacgaaaaaa acgtgggcga cctgctcaag
     8761 caggccgacc tggcgatgta ccaggccaag ggcgcggggc gcaacaccat ctgttttttc
     8821 gaccccggca tgcaggcggt ggcgacggcc aacgcggcgc tgacctacga gctgcgccag
     8881 ggcctgcgca acgaggaatt cgtgctgcac taccagccgc aggtcggtcc caagggtttc
     8941 atggtgggcg tcgaggccct ggtccgctgg cagcacccgc agcgcggcct ggtgtttccc
     9001 gatggtttca ttccccaggc cgaggaaagc gggctgatcc tgccgctggg caagtgggta
     9061 ctggaaaccg cctgcgccca gctggcgcgc tgggccgcct gtccggaaac cgacagcttc
     9121 agcatcgcgg tcaacgtcag cgtgcgccag tttcgccacc ccgagtttgt cgagcaggtc
     9181 atggcggcca tcgccagcgc cggcatcagc gcgcaccggc tcaagctgga gctgaccgaa
     9241 agcctgctgg ccaacgacat ggacgtgacg attgccaaga tgggcatcct caaggacgcc
     9301 ggcgtgacgc tgtccatcga cgacttcggc atcggctact cggcgctgtc ttacctgaaa
     9361 cacctgccgc tggatcagct caagatcgac cgcgcgtttg tgaaggacgt gctgaccgac
     9421 cccaacgacg cggcgatcgc gcgcaccatc atcggcctgg cgcaaagcct ggggctgggc
     9481 gtgatggccg agggagtgga gaccgaagcc cagcgcgact tcctggcccg ccacggctgc
     9541 cactgcttcc agggctacct gttctgcaag ccgctgccga tcgacgaact cgaagtgttc
     9601 atgcagggca tggccaagtc gccggtgggc acgaagggcg gctgagcccc gctgcgcagc
     9661 gccatcaaac cgctactgaa ttcatagcta ctggcgcagc agccacaggg gctggaggca
     9721 cttttttctt gcgcctgccg ataacggcat ttttctcgac cagctaacca acctcgctgc
     9781 cgggtttggg cacgaccagc aactcgcaca gatcagtgaa gtaactttgc gcgccctgct
     9841 cttcgttgaa gtgacaggcc gggccaccgg ggccccattt ggcgctaaag ggaacggtca
     9901 tggttttctc agtagccgtg cagggcgcag tcaagggccg accagatgcg tggggcttcg
     9961 ctgcgcaagc tggtgacctc gccccgcaga tcagcggatg gaaacgcgag caagcccaag
    10021 gtgtccagaa acagcgccac gccttcgacc tggaccacgg gggccaggcg ttcggtgggc
    10081 gactccaccc aaacgtgcgt caggggaatg gtcaagcggt tgcgcacgcc gcgcacgagg
    10141 tcactttgaa tatcgaccac atagggcgac tgatctcgcc actcttccac gggatgaggg
    10201 taaacgtcga attgcgccat cagccgggct ccgcgcccgg ggctttgaag gccgcctgct
    10261 ttttgcgcca ttcgtaaatg cgccagccca tgctgccttc ccgctcgatg cggcggttgt
    10321 aggcagcaat cgtgtccttg ttctcggcct cccactgcac ggccaatcgc tcgatttcct
    10381 cggggctacg ctcctgcgcc gccttgcgcg ccgccagacg ctcaggcgtg agccagccgt
    10441 cgccaacgcg gtagctgccg gcttcttcct gcacgccggc aggtgcagag gcgtccgtgg
    10501 ggtcctgcac ggcaggcaca gccttcatgg gcaccagttc cacccggccg tcggggtaaa
    10561 acaccacgtc aaacaaccgg cccgcgtatt ttttacccag cgagatctgg ccgctggcgc
    10621 cgacttcctt gagcatggcg catcccttta aacgttcatg ccatcatgcc gcatgaaatt
    10681 tggggcgtca aattctgccc agactgaagc gcttatgtgc atggcaaggt caattcaagt
    10741 ccttattgtt tccaatatgg caatattaaa ttttattgaa tgctattttt ctttgttatc
    10801 ggcaacagta aagttcatac atcgatgagc cgcaaccaat aaacgactca ccgaggctgg
    10861 acagacgggg atcaaaaagc ccgccatgcc cggaaatgtt tattcactct cacaaggaaa
    10921 tttcatcatg aatgcatcca aattcttcgc tgttgccgct cttgccactg ttgcctcttt
    10981 cggcgcccac gctgacgaag ccgacggttc gcagtttggc atccagttca actcgacccg
    11041 cgctgtggcc gaagtgcgcg ccgaagccgt cgccaaagtg aaaaaccaca gccaggagcc
    11101 tgccggctcc cgcgttgcgg ccaccgtgca gtcttcgacc gaccgcgcca ccgtgcgtgc
    11161 acaagcggct gacgcggtcc gcctgggcca gatcccgcgc ggcgaagcca gctacatgta
    11221 atggctgcag gctgaccggg tttccggccg gctgatcccc caccacccgg gctccggctt
    11281 tggcagtggg tgaatctttt gaaatcgggc tcttcagagc ccgattttct tttcgggaca
    11341 ggaagctaag gaagcgctga ataaatcctt gcggaggcgc ggcagccgga tcgggatggg
    11401 atgcaaggcg gatttcgcag
//
LOCUS       DAIDFG010000070        11383 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_70_length_11383_cov_17.399011, whole genome shotgun sequence.
ACCESSION   DAIDFG010000070 DAIDFG010000000
VERSION     DAIDFG010000070.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 11383)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11383)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11383
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70_length_11383_cov_17.399011"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2031 a         3724 c         3502 g         2126 t
ORIGIN      
        1 cattctggcc ggccacagcg ccggcatcgt gctggaccgc gaggtggacg tgtcgcgcgg
       61 cgactggctg ctggctgccg acacctttga acccacacgc gaactgtcgg tcaccatcgc
      121 ctggatggac gacgaaccgc tggtcgccgg acgcgtctac tgggccttgc acggccaccg
      181 ctgggtgaag gccaaggtca aacgcatcgt gcacaagctc gacgtcaata ccctggccga
      241 ggaagacgcc agcgagctgc cgcccaacgc catcggccac atcgagctga gcctgcaggc
      301 cccgctggcc accctgccat atgcccgctc gcgggtcctg ggctcgctgg tgctggtgga
      361 cacggcatcg aacaagacct cgggcgccgc gctgattctt tgatcaggat cagccccgat
      421 caagcacccg acttaaccct gctcacggaa tcctcttaaa attgagggtt tccaccgatc
      481 ccgcaagaac aacatgactc acatcgttac cgaatcctgc atccgctgta aatacaccga
      541 ctgtgtggat gtctgtcccg tggactgctt ccgcgaaggc cccaacatgc tggtgatcga
      601 tccggacgag tgcatcgatt gcgcggtctg catcccggag tgcccggtca atgccattta
      661 tgccgaggaa gacgtgccgg ccgaccaggt caacttcatc aagatcaatg tcgagctgag
      721 caacgcgccg ggctggaaaa gcatcaccaa gcgcaaggaa gccttgcccg atgccgaaga
      781 gtggaaagac gtcaccagca aactggacca gctcgttcgc tgagacgtac ccgccgcgga
      841 ttacgcgcct gcctgccctc ttcaaactgc aacccccaaa aaagccccat ggaacctcaa
      901 ctgaaccagg aagtgatcaa cacggctgtc gcgcacgatg gcccgataga gaccgatgcg
      961 gtcatcgtcg gtgcgggacc ggtcgggctg ttccaggtgt ttgaactcgg cctgctggaa
     1021 atcaaggccc atgtcatcga ctcgctggcc taccccggcg gccagtgcat cgagctctac
     1081 ccggacaagc caatttacga cattcccgcc gtgcccatgt gcaccggcaa ggaactgacc
     1141 gactccctgc tcaagcagat cgagcccttt ggcgcgactt tccacttcgg ccaggaagtc
     1201 agcaccgtcg aaaggcagga cgacggccgc tttttcgtcc agactgccaa aggcacgcag
     1261 ttcctgacca aaaccatctt tattgcggcc ggcgtggggg ctttccagcc gcgcctgctg
     1321 aaggtcgacg gcctggagaa gtttgacaac acgcagctgt tttaccgcgt caagtcaccg
     1381 gctgactttg ccggcaaaaa cctggtgatc gtcggtggcg gcgactccgc gctcgactgg
     1441 gcgctcaact tcgccaagga aggcccgaac aaggccgaaa gcgtgatcct gatccatcgc
     1501 cgcgacggtt tcaaggccgc cccggccagc gtcgcaaaga tgaaggagct gtgcgacaac
     1561 tacgagatgc agttcatcgt cggccaggtc acaggttacg aggaaaagga cggcaagctc
     1621 accggcgcca aggtcaccgg tgccgacggc gtgacccgcg tggtgccact cgacgtgatg
     1681 ctggtcttct tcggcctgtc gcccaagctc ggaccgatcg ccgagtgggg cctggacatc
     1741 gagcgcaagc agctcaaggt cgacaccgaa aaattctcta ccaatgtgcc cggcattttt
     1801 gcggtcggcg acatcaacac ctaccctggc aagaaaaagc tgatcctcag cggctttcat
     1861 gaatgcgcgc tggcggcttt cggcgcggcg cctttcatct tcccggacaa gaaaatccac
     1921 ctgcagtaca ccaccaccag cccgaaactg cacaaggtac tcggggtgga gacgcccgtt
     1981 ttcgactgaa caccgggcta caaccccctg tcaggcccgc caagtgcggg ccttttcttt
     2041 gtctgtcgtt ttttcgatgc aattttatcc gggtaatatt gacagcctat ttttacccgg
     2101 gtaatatcca gtccattgaa aaagaggaca ggccggcgac cacggcaggt caggcatctc
     2161 ttccatctgg tcctgtcagg agagaacatc atgggaaccc ttcatctggt cttcggacca
     2221 caaggcgccg gcaagtcaag ttatgcgcgc caactcgcag cgagcttcaa gggcgtccgc
     2281 ctgtcgatcg acgactggat gggccagctg tatggccccg acctgcccac acccatgaac
     2341 tttgcgtgga tcatggaacg cgtccagagg tgcgagaagc gcatctgggc caccgcggca
     2401 gaaattgccg gcacgggcgg ccacgtggtg ctggacctcg gattcatgaa ggtcagaaac
     2461 cggactgaat tcctgtcgct ggccagcgcc ggccagcatc cggcgcagct tcactttgtc
     2521 aacgccccgc tcgatgtcag gcgccagcgc gtgctccaga ggaacagtga caagggcgac
     2581 accttttctt tcgaggtcag cgcgcccatg ttcgatttca tggaacgcga gttcgagccg
     2641 cccacggagc aggagctggc cagtgccacc gtgttccatt cacactgaaa gaaaccatgc
     2701 ccccatcgtc cggccccatg gccagcacct ccccgcagcg gtgttgcacg gccttcgagg
     2761 aaagccgccg tatcgccacc ggccccttgc gcgaggtcgc cctgaaggtc aaggccgtcg
     2821 ccgaccaggg ccggcagaaa accattctgg tgtttgacga cctgacgagc gagctggtcg
     2881 agctggacct gcgcggcagc gacgccgatg tgctggaccg cctgcaagcg cccgggcttg
     2941 cggagggcgg cgatgtcccg gccacgccgg agccggagga cgcaccgcgc ggccccggcc
     3001 ggccccggct gggcgtgatc gcacgcgagg tgaccctgct gccgcggcac tgggagtggc
     3061 tgggcagcca gcccggcggc gcttcggtgg ccctgcgcaa gctggtcgag gaagcacgcc
     3121 gcacccacga gggcaaggac cgcgcgcgcc gggcgcagga ggtggcctac cgtttcatgt
     3181 cggccatcgc cagcagtctt ccagggtttg aagaggccac ccgcgccctg ttcgcgggag
     3241 acagcgaccg ctttgatgcg caaacccaag cctggccgcc cgacctgcgc agccacgcgc
     3301 aaggcctggc ccggcaggcg ttcatggcac cggaagaaaa aaagggcgtg gcatgacaac
     3361 acgacctccg ctgatgtgga aggccttcct cgtcttcctg ggtcccatga tggccagcaa
     3421 catcctgcag gggctgtcgg gcacgatcaa caacgtctac ctcggccaga tgcttggtgt
     3481 cagcgccatg gcgtcggtgt cggccttctt tcccgtgatg ttcttcttca ttgcctttgt
     3541 catcggactc ggcgccgggg cctcggtgct gatcggccag gcctggggtg cccaggagcc
     3601 ggaccgggtc aaggccattg caggcaccgc gctgagcgtc ggcctgctgg ccgggctggc
     3661 cgtcgcggtg ttcggcggaa ccttcaccgg ccggctgctc accctgctgg gaacaccggc
     3721 tgatatcctg cccggttcca tcgcctatgc ccgcgtgatg ctgatcgcca tgcccggcct
     3781 gtttgtgttc ctgctggtca cctccatgat gcgcggcgtg ggcgacacgt tgacgccatt
     3841 gctggcactg ctgctgtcca ccgcggtagg cctggtcgtg acgccggcgc tgatccgtgg
     3901 ctggggcggc ctgccgcaat tgggggtcgc cagcggcgcc tggggtgcgg tgctgtcctt
     3961 cacagtggcg ctggcctggc tggcctggta cctgcggcgc aagggccacg cgctggcacc
     4021 cgacgcccgc tttctgcagc acatgaagat cgaccgcgcc atcctcggga aagtcctgcg
     4081 catcggcctg cccaccggca tgcagatggt catcgtctcg cttgccgaag tggcggtgct
     4141 gttcctggtc aaccgcttcg gctccgatgc caccgccgcc tacggcgccg tgaaccagat
     4201 cgtggcctat gtgcagtttc cggcgatctc catcgccatc acggcctcca tcctcggggc
     4261 gcacgcgata ggcgcgggcc atgccgagcg cctgggcacc atcgcgcgca cggccatcat
     4321 gctcaatctg gtggtgaccg gcggtctggt gttgctggcc tacctgttct cgcgcagcat
     4381 catcggcctg ttcatcacga gcgcaccggt catcgaaacg gcccagaccc tgctgcacat
     4441 catgctgtgg agcacggtca tcttcggcat gtcggcggcc atctcgggtg tgatgcgggc
     4501 cagcggctcc gtgctggtgc ccaccgcgat ttccatcacc gccattctgg caatcgaggt
     4561 gcccgtcgcc tggctgctga gccgctccat cggcctcaac ggcatctggg tggcctaccc
     4621 cgtagcgttc accgccatgc tggtgatgca gaccgcttac taccggctgg tgtggcaaca
     4681 caagccgatc cgccggctgt gagccggcgg cgcgtcatgc gggtcctgcc gtagaatcca
     4741 actcgcgttc ccggaggagc gcattcgctg ggggtgttgc cacgcaagtg gcgactgaga
     4801 aagtcccttt gaacctgact gaggtaatcc tcgcgcaggg aagcatgtcc aaggcgcgct
     4861 gcaggttttt cgaccgcgca cgcgccctcg caagccgacc tgccattgac acaaggaaaa
     4921 gttgatggcc accctacccg ctacccccaa caccgcactc acgcccctgg cccccggaga
     4981 ccgcctgttc ggctggcgcg aacacgccgc actctggctc agcctgggcg tcggcctgct
     5041 ggtcatgcag atcggcgcct acctggtccc ggcgctcggc gccaaagccg ccatggccgt
     5101 gattgtggtc ggatccatcc tcggcgccgg cctgctggcc tggaccgcga agatcggctg
     5161 cgacagcggc ctgtcgagtg ccggcctgat gcatgccacc tacggcagca gctttgcgcg
     5221 cctgccggtg ctgctcaaca tcgtgcagct gatcggctgg accacgtttg aactggtggt
     5281 catgcgcgac ggtacggtcg ccatcgcccg gcagtcgggc tcgtttgtcg cgtccttctg
     5341 gccggtgctg gccaccctgc tctggggcgg cgtgctgatt gccctgctct cgggctccat
     5401 ggtcaagctg gtgcgcaaat tcataggccg ttatggcctg ccgctggtga ttgcctcgct
     5461 gctgtggctg acctggcagt tcctcggacg cgcccacgcg caaggctggc aggacatctg
     5521 gaatcgcccc ggcaccggcg gcatgagcac gctgtcggcc ctggacctgg tgattgccat
     5581 gccagtgtcg tggttgccgc tggtggccga tttcgcacgc catggccgcg acggcaaaag
     5641 cgcgctgcgc ggcacctggc tgggttacgc gattgccaac acctggtgtt acggcctggg
     5701 cgtgctgatt gcgctgacca caccaagcac cgacctggtt gccgccctgc tgctggcgca
     5761 aggcgggctg atcgccctgg gcctgatcct gatcgacgag gtggacaacg cctatggcga
     5821 cctgtactcc ggttcggtcg cgggccacag cctgaaaccc ggctggagcc tgcgccgctg
     5881 gggcatggcc ctcgccgtcc tgtgcaccgg cctggcgctg gtgctgccca tgcacagcct
     5941 ggagccgttt ctgctgatgc tcagttcggt gtttgtgccg ctctacggcg tgatcctggc
     6001 gcgcctgggc ggtgcgcaga atgtggccgc gctggtggga acgaaaaaag tcaatgtcag
     6061 cgcggtcgtg atctgggtgc tgggcgtggc cacctaccac ctgtgcgcca aactcgcgcc
     6121 cgagtggggc gcggcgctgc ccacgctggc gctgaccttt ttgctcgccc gtgtgacccg
     6181 tgcccattcc gggccggcta ccggagaacg cgtactcagc cccgcggctt gagcggcttc
     6241 aagggcttga gccgcatggc cagcggcgca tgcccgtggt tcagcggccc gctgccggcg
     6301 ccggttttta ccttggcgcc ggccagcagt gccgcacgca cgtaggcccg ggccatttcc
     6361 acggcttccc gcaagggctg ccccaacgcc agtccggccg caatggcgga agaaagcgtg
     6421 cagcccgtcc cgtgcgtgtt ggcactgtga atgcgcggcg catgcatcca gtgcctttcg
     6481 cccgctgccg tcagcagcag gtccatcacc gtgtcgcccg gcagatgccc gcccttgagc
     6541 aacaccgcgc gtgcgccctt acccaaaagt tcgtgggcgg ccgcttccat gtcatctgca
     6601 ttgttcagcg gccgcccgac cagcagggca gcttcgtcca gattcggcgt gaccagcacc
     6661 gcgcgcggaa acagctcccg caccagcact tcaatggccg ggttgtcaat cagcaccgcg
     6721 ccgctggtgg ccaccatcac cgggtcgagc acgatctttt caagcgcatg gcggtcaatg
     6781 gcttcggcca cggccagcac gatctcgggc gcatgcagca tgcctatctt cacggcgtcc
     6841 acaccgatgt cctcgaccac cgcatcgatc tggtcgcgca gcatggccgg cggcacgcca
     6901 tgaatggccc gcacgccctg ggtgttctgc gccgtcagcg cggtgatggc cgtcatgccg
     6961 taacagccga gcgcggcaaa agtcttcagg tccgcctgga tgcccgcccc gccgccgctg
     7021 tccgagcccg caatggacaa cacgcgcggg tactcgaatt caaaagtgtc gttgctcatg
     7081 gagaaaagtg tttcccggtg gttcaggccc tgcgaaggcg atgccggatt atcggggaag
     7141 ccgcttggcg ccttgcgccc cggggatcag cgcctgaatt ctcggcccag agttcaaagc
     7201 tgcgccagaa gtccgaaaaa gtcaggctat aatcttaggc tgtattcctc aatagctcag
     7261 ttggtagagc gccggactgt taatccgtag gtccctggtt cgagcccagg ttgaggagcc
     7321 aagatttgaa gcctggacgt gcacaacgtc caggcttttt tcgttggcgg ggttaccatt
     7381 tgtgggtgcg gttcttgcgc caccggcgca tgcgctcagc acgccaaatc acacccttgc
     7441 cccaccctcc tgccatgcgc aagtcacccc accccctgcc ctggctggat gcggacgaac
     7501 tttttccgcc ggtggaaacg gcgtgggccg agggtgatcc ggcgcccggc ctgctggctg
     7561 cggggcgctc gctcgatgtg gcaaccttgc gccaggcgta tgcgctgggc atcttcccgt
     7621 ggttcggggt cggccagccc atcctgtggt ggagccccga cccgcgcatg gtgttgcgta
     7681 ccgacaattt caagctgcac cgctcgttgc gcaaaagcct gctgcgtttc atcggcgcag
     7741 ccggtcatga gattcgtttt gacacggctt ttccggaggt gatccacgcc tgtgccagca
     7801 cgccgcgcga cggccagccg ggcacctgga tcgtgccgga catggttcag gcctataccg
     7861 ccctgcaccg cgcgggccat gcccacagcg tggaaacctg ggtgaacggc cggctcgtcg
     7921 gcgggctgta ctgcgtcaac ctgggtggca tggtgtttgg cgagtcgatg tttgcccacc
     7981 agaccgatgc gtccaagatg gcgctggcgg cactggtggc tttctgccgg gccaagggcc
     8041 tgcccctgat tgactgccag caaaacacgc ggcacctggc ctcgctgggc gctgcggaaa
     8101 ttccccgcgc ggaatttgcc cggcaggtgg ccaccctcgt ggctcaagcg ccaccccgct
     8161 gggagtttga cgccctatac tggcagcagc tattgacggc cagaccgcag aatgacttga
     8221 cgacgtgacg cacctcaaag acctgcccct ccagacgctg cagttttatg cgacagcgcc
     8281 ctacccctgc agctatctgc cgggcaggca ggcccgctcg caagtcgcca cccccagcca
     8341 cctgattcac aacgccgcct actcggagct ggtgaccaac ggctttcgcc gcagcggcat
     8401 gttcacttac cgcccctatt gctacggctg ccaggcctgc gtgcccctgc gcgtgccggt
     8461 ggcaactttc cagccggacc gcagccagcg ccgcgcccgc aagcagcatc agacgctgca
     8521 ggttcgcgtg ctcaagctgt gtttcatgcc cgagcactac cagctctacc tgcgctacca
     8581 gaatggccgg cacgcgggcg gcggcatgga ccacgacagc atcgaccagt acacgcagtt
     8641 tctgctgcaa agccgggtca actcgcgcct cgtggagttt cgcgacaccc tgcccaatgg
     8701 cgaggccggg gcgctgaaaa tggtgtccat cctcgacgtg ctggaagacg gtgtgtcggc
     8761 ggtgtatacg ttttacgagc ccgacgtgca caccagttat ggcacctaca gcgtgctctg
     8821 gcagattgag caggcccggc agctgggctt gccgcatgtg tatctcggct actggatcgc
     8881 cgaaagcccg aagatgagct acaaggccaa gtttttgccg aaccagatcc tggtcgatgg
     8941 gcaatgggtg aactctgcca ccccgggccc agcgagcgcc gcctgagagc accgtctgtg
     9001 aggcaggtca agacgaagcg gcgtccaata gcggcgtgct gatttcacca gctaaaatct
     9061 ggtgaaggtt acccccatga aaaagcaaga ttccctcgca tcacccgcca aacccacggt
     9121 tcaggtcatt gaacgcatgt tcaccctgct ggagattctg gcgggccggg aggaggccgt
     9181 ctcgctcaag gaaatcagcg aaaaatccgg tctgcacccc tcgacagcgc accgcatcct
     9241 gaacgacctg gccaccggcc ggtttgtaga ccggccggtg gcgggcagct accggctggg
     9301 catgcgcctg ctggaactcg gcaacctggt gaaggcgcgc ctgagcgtgc gggacgcggc
     9361 gctgacgccc atgcgcgagc tccaccggct gatccagcaa cccgtcaacc tgagcatgcg
     9421 ccagggcgat gaaatcattt atgtcgaacg cgcctacagc gaacgctccg gcatgcaggt
     9481 ggtacgcgcc atcggtgggc gcgcaccgct gcacctgacc tcggtaggca aactgtttct
     9541 ggcttttgac gatccccagc gattgcgcag ttatgccacc cgcaccggtt tgccgggcca
     9601 gaccaaaaac agcatcaccc agttggcggt tctggagcgc gaactgtcca aggcacgcca
     9661 gtacggcatt gcccgcgaca acgaggaact ggagctgggg gtgcgctgca tggccgccgg
     9721 catttacgac gaccagagca agctggtggc gggactgtcg atttctgcac cggccgaccg
     9781 gctggatgaa aactggctgc ccaaactgca gtccaccgcg agcgagatct cggcggcgct
     9841 gggctacaag cccaacaatt tcaagtaaaa acgggcccta gcccttgtcc ctattgcgct
     9901 ggtagctacg ttttttatag caattcagcg gaccggccgg tctaccgatc agtgaaaaaa
     9961 gtgggcctca gccgttattc ggctgggtgc ttgcgctgat tttgggggcg ttgaccggtt
    10021 gggactccac ccagcggcgg acgcgctctg cgtcaccgag gcggctcaat ttgccggccg
    10081 aatcaagaaa aaccatgatc agcttgcgcc ctgcgatttt ggtctgcatc acgaggcact
    10141 ggccggcttc ggagatgtag ccggtttttt gcaggccgat ttcccactcg ggatttttca
    10201 cgagccggtt ggtgttgttg tactgcaggg tctggcggcc gacagcaacc tggtagccgg
    10261 tcgaggtgga caactcgcgc agaaccggat cggcatgggc cgcgttgacc agcgtcgcca
    10321 ggtcctgagc gctcgactgg ttctggctgg acagccctgt gggctccaca taacgggtgt
    10381 ctttcatgcc cagcattctg gccttggcgt tcatcaagcc cacgaaggta gccaggccgc
    10441 ccgggtaagt gcggccaagc gcatgcgcgg cgcggttttc gctggacatc agcgccagat
    10501 gcagcagctc gccacgtgtc aggctggcgc ccacgcgcag gcgggagctg ctgccctttt
    10561 cggtgtcgac gtcgtcctgg gtgatggtga tggtttcgtc catcggcaga tgcgcgccgc
    10621 tgacgatcac gcccgtcatc aacttggtca gcgaagcgat gggaagcacc gcatggtcgt
    10681 ttttgctgaa cagcacttcc tgggtgtcct ggtcaatcac cagggccacg ctggatttca
    10741 ggtcaagcgg gtcctgtgca ccatgcaggc ccgccagctg gccatatgac agccggggcg
    10801 gcgaggccac gatcaccacg gacctgcgcg aggatttgga gacgaagcgg gcacttttcc
    10861 ggctggaaat cacctttttc gacaccgtct tggcggcgtg tgacttttta acgatctggc
    10921 gcttcgccgt ggcggcgtct gccgcgggcg aaatcaaggc cgccgaaaag gtaagaattc
    10981 caacgacctg taaggtctgt ttgaaaatcg aattcagcgt gatggacatt gattcgtctc
    11041 cagatgcgat tagtacgaaa ccgcagtgta tacaaataga aaaagtcacg caatatcaaa
    11101 agcttgcgtg actttcctaa acgcaacagt gaagagatcg gtccatgcgg cttttcagcc
    11161 cacgccaacc cacaaaaact tactaaactg ttgaatataa aaggtttttt tgaaaccttc
    11221 cggcctcaac cctgggcagc aacccgatca gctttacttt gtaacttgct caaggcgctc
    11281 aaataagcct tggcagaggc gaccacgatg tccggatcgg ccccgacgcc gttgaccaca
    11341 cggccgctgt tttgcagacg caccgtgact tctccctggc tct
//
LOCUS       DAIDFG010000071        11262 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_71_length_11262_cov_17.183457, whole genome shotgun sequence.
ACCESSION   DAIDFG010000071 DAIDFG010000000
VERSION     DAIDFG010000071.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 11262)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11262)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11262
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71_length_11262_cov_17.183457"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2255 a         3331 c         3391 g         2285 t
ORIGIN      
        1 gttgactgcg caaaggcagg taccgatata aaaaacagtg tgcaggccag cgccagcgtt
       61 ttcggtcgca tcgaattctc ctagttgcga aaaccaaggg gggagaatcc ggcgagaagc
      121 cagctaccga agggcagcta aaaagaaaag gagcgttctc gccggactaa gcggcgaggg
      181 tccaattagg tcgttcgatg acgtccggca ccctgaactg ggtcggcaag ggatcgggtg
      241 gttcgaaaac cgctggcgac gcaacaggaa cactcaaggc attcccggga agcggctgag
      301 cgcatcctat gtggcagtag ttgcaatccg ggtcgtccgt cccgcccaac gcacccgtca
      361 aggcactctc gggtattgaa accgtatgca cgagtgcgtg atgaccaaaa tggctcgtct
      421 tgtcgatatt ttcgtgcacg caataccttg ccgcagcgcc ccaggcgaac tggagcggca
      481 gaaatacgat gaagaaaatg atcagataac ggcgcacgat gagagtgtac aaggcaaatg
      541 tttcagggac ttgcaggtgt ggtcattacg gtatagtttt ctggaaaaag ccgctcaatc
      601 aactccagcg ggctgcgaca tctcttggct cgttaatgca atcacggtca gacttcgttc
      661 catagccgcg acggcagcac taacgacatt ggtgcatttg ccgcttgtgc taatagccag
      721 ctatcagtct cccggtcggt cgtgtgcatc caggtatctt gagaaaacgt gttcacaccc
      781 aaagtcattt gcggcgcagg tcaagcgacc aacgtcgcta atttgagggt ggccagaata
      841 gtttcacgca tggctggaat ggtgggctgg ttcatcctgt agatttatgt tttgggagtg
      901 gtggcggaat ttttcgcacc gcgcagcaaa cgaaggccat tgccgacaac cagcaggctt
      961 gcacccatat cggcaaagac cgccatccac atggtcgcgg atccacagtc gcattgcgca
     1021 cagccatgcg cattttcctg atgcccacca tgagggttga caggtagcct gatccaatca
     1081 gcaaagttca acgagagttc cccataggga cggatcgagt tggtaaaaaa tggctggcgg
     1141 aaaacctggg tctatgagtg gtcaacctca gcaaggtggt caacgaataa cggcgacaag
     1201 ctcggatcca tcgatgacct tacgatcgaa aaacgttccg gccaaccttg gccaccacgc
     1261 agcgggaatc tgtcacaacc cgtaaaaccc tctccggtgg tcagtgccgg tccaggttcc
     1321 acaatttgac tctccccagc gcttcacctg gcgtgaccga gaggtcgatg gcacgggtga
     1381 tcactgcatc acggcggaat tgctcgcgca ctgctcggta gcggtggagg atggtggagc
     1441 ggtcaactgt cacgaaaagc tccgcgtcgg cctccagggc cgcctccatg tgctcaacgt
     1501 cgaattccct cttgcgtgct agcagctcaa ataaatgcag gtctgggctc agggttgcct
     1561 tcgcctgggc cagccgatcg tcgtgctgat acctacaggg gcaacatcca gccaagcttg
     1621 ttcctcgacg gatcaccaca agagccttcc gtgaaaacgg cgatcaaaca atgtggaaat
     1681 gcggcgctta ttggttcggg tacgcatgga aacttcccac tggcaggact gcccggtcag
     1741 gcacatcatt gcaggctcaa tggtagacat gggtaagtct tggcacacat ggtagccgct
     1801 cccgatttgg ctttaggcga acgtgcattt accgctgtcg ctgccggtaa gcgcgccggg
     1861 cgccgtctca agcatccacc tcgcgaccgc tgagccgtga cacctcccct gacctcgatg
     1921 catcaattca gcgccgaatt ggcgtgtgcc cttgagactg gacctcattc aaaacatgga
     1981 tatttcaata cttcaacaat agttgtacta ttgatctatg gaagagctag acgcaatcaa
     2041 ttccctggcc gccctggccc agacccaacg tgtgcggaca ttccgcgcct tggtggtggc
     2101 cggacctgca gggctaaccc caggagccat tgccgaacaa cttaacgtgc caggcaatgc
     2161 gctctcgttc cacctcaagg aactgtcgca tgccgacttg gtgagcgttg agcaacgtgg
     2221 ccgcaatctg atttatcggg cgaacttcgc ccaaatgaat gccctcctgg gctacctgac
     2281 cgagcattgc tgccaaggcg cggcttgtga ggttgttgac tccgcctctt gtgttaactg
     2341 ttgaaaggaa ccagcatgaa acgctttcat gtccacgttc acgtggatga cctcccgaga
     2401 aacatcgcct tctattcggc gatgttcgat cagcaaccgg cacgcaccga gaccgactat
     2461 gcgaaatgga tgctgaccga tcctccggtg aatttcgcga tttcgacgcg aggcaatgct
     2521 gcaggtgttg atcacctggg tatccaggtg gacaacacgg acgacctgaa ggttttgcgc
     2581 agctgtgccg ctaaggccga catggggatt cttgacgaag gcgctaccag ctgctgctat
     2641 gcgcgaagcg agaaatactg ggtgacggat ccccagggca ttgcgtggga gcagttccat
     2701 acgctggact cgattcctgt ctatggcgag agccgggcaa cggacgatgc tgcagccgga
     2761 gcctgctgcg tgggtgagct cactgcagcg cccgtcccgt ttgtcaacgc acgccccggc
     2821 gttggcgccg gcaagtcctg cggctaattg gatgcgccct atgagcactg tgaaatctat
     2881 caacgttttg tttctctgca cgcacaattc ggcgcgcagc attcttgctg aggcgacgtt
     2941 gaaccacctg gggaaaggcc gcttcaaggc cttttctgca ggcagcaacc cgcgtgaaaa
     3001 tcagcaaccg aatcctctgg cgctaaagat tcttgagaag gccggtattt cgaccgaagg
     3061 tcttcgcagc aagagctggg acgagttcgg caagctggat gcaccgcaca tggacctggt
     3121 catcacggtc tgcgacaacg ctgccggcga ggtgtgcccc tattggcctg gccaaccggc
     3181 gacggcgcac tggggctacg ctgatccgtc cgagacgtcg ggcacggatg accagaagct
     3241 ggaagctttt cggcagactc tgcacgctat tcgtcggcgc ctggacctgc tagtgaactt
     3301 gccagctgcg ggtgtggaca agctgatgct gcaaaccaag gccagggata tcgccaagtc
     3361 ctgaagagtt gacgaacgag cgtctgggaa ttatttcact gtcgtcatga cgtaatcgtc
     3421 gccttctttg acgaacccaa agtcaacctt cttgtccacg gccaatttgg cgagcagatc
     3481 cttgtccttg accttaaaac tcatggtcat cggcggccag ttcaggctgt tcactggccc
     3541 atggacgatc aggacgtagc ctgtcttggc attgatcttc ctgatcgtcc cgttggcctc
     3601 atgcgtggtc tgggcgttgg cagcgggttt cttggccatg tccatgctct tcatatcgcc
     3661 cattttcgac tgagcgaacg cggcggatgc ggcgacttaa acgagagaag cgattgcgat
     3721 ttttttcatg aaaaactcct cttgcaagag gtgtaatggt taaatccggt tgtccggcga
     3781 gataaatccg tcatgggctg catcttcgtg cagtgcatga cggaaggagc gtgaccaatc
     3841 ccatctcaaa agttacgtgg tactggtttc tttggcggca tctttgcagg ccttgacttc
     3901 ggcctcataa cgctctttgc gcagtttgtt caaatatggc gttgtctgcg cgcgcgggcc
     3961 atggtcgagt tgcaatacga gtttttttgg cggacagacc ttgacctggt ctggggcagc
     4021 actggcaggc aaactgatgc caagtgtcgc ggcgctaaca agggcgataa gtagggattt
     4081 catgatggtc tccttggata aggaaaggaa ctggatgggt aattgagcgg gacgcacggc
     4141 tggtgactaa ttgcgccagc aacagtacgc gacgcgcaaa ggcaggcccc cctctcgctt
     4201 tggcagatcc aggaggtttt tcgttgacgc agaagactgc gccagcaact gccatacagc
     4261 ttgtttgggc tcctcttgct cgccagtgaa cccatgcacg gctggtgcgg gggccttgta
     4321 gcagctcgcg caaggcaagc cgttcattgc acctgacatc ggattctttt gtgcgccagc
     4381 ttgtgtcacc tccggcacgg ctttcatggt caagctcgcg gcctggactg ggttcgcaag
     4441 cccagccgca aacagcacaa gcgccgccat gaggagcgcg cgtcgcagcg cttgatcgag
     4501 gtgatgctgg cgttcaggcc gcttcacgat gccttctcca aaagaaccac tgtcgggtat
     4561 ggctcgtgcg cggccggcgt atcagcagat acaccgctgg aatcacgaac atcgacaaca
     4621 gcggcgcggt gatcatgccg cccaccatgg gcgcggcgat gcgctgcatc acctcggagc
     4681 ccgtccctgt gccccacatg atgggaaaca ggccggccag aatgaccgcc accgtcatgg
     4741 ccttgggccg aacgcgcaat accgcacctt cgcgaatggc gtccaacaag tcagagttac
     4801 tggttttccc ttgttccagc cgatcgtccc aggcagactt caggtagagc agcatgatca
     4861 cgccgaattc cgccgagaca ccggccagcg caataaatcc aaccgcgccc gcgaccgaca
     4921 ggttgtagct cagcaggtag agcagccaga tcccccccac cagcgcaaag ggcagcgtgg
     4981 ccaagatcag cagggcctcg tcgaagcgct tgaaggtcag gtacagcagg acaaagatga
     5041 tcagcagcgt gaacggcacc acaaccttga gcttggccgt ggcacgctcc aggaattcga
     5101 actgacccga ccaggagatc gaatagcccg gcggcagctt gaccttgtcc gccacggcct
     5161 tttgcatgtc ctgcacggcc gagcgcagat cacgtccgcg gatgtccacg tagacccagc
     5221 ccgagaggcg agcgttttcg ctgcgcagca tgggtgggcc gtcggtgatg cggatatcag
     5281 ccacgtccga cagcaccagg cgctggccgc gttccgtcac aaagggcagg ctgcgcactt
     5341 tctccacaga atcacgcaac tctctggggt aacgcatgtt gatggggaag cgctgcaggc
     5401 cctcgaccgt ctcgccgatg ttctcgccgc ccacggccga actaatgatc gactgcacgt
     5461 cggcaatgtt caggccataa cgggatgctg catcgcgctt gatattgacg tccacataac
     5521 gcccaccggt caagcgctcg gccaaggcgc tcgacacgcc gggaacatct ttgagcgcac
     5581 gttcgatctc gccggtcagc cggtctatcg tgatcaagtc ggtaccggct accttgacgc
     5641 ctaccgggct cttgatgccg gtagccagca tgtcgatgcg gttgcggatg ggcggcaccc
     5701 agatattgga cagaccggga accttgacga tgcgatccag ttcatcgacc agcttgtcct
     5761 gcgtcattcc cggacgccac tggtccattg gcttgaactg gatggtggtt tcaaacatct
     5821 ccatgggcgc cgggtcggtc gccgtttcgg cgcggccggc cttgccgtac acgctggcga
     5881 cttcgggcac ggtcttgatc agccggtcag tctgctgcag caattcactg gccttgccgg
     5941 ctgacaagcc tggtaacgcg gagggcatgt agagcaaatc accttcgtcc atcctcggca
     6001 tgaactcccc gccgatatgc tgcagcggcc agaggctgat caccagaatg atgccggcaa
     6061 ccagtaatgt gagcttgggc gcgcgcagca ccacttccag catcggccgg tagatggcga
     6121 tcagcaagcg gttcaggggg ttggacttct catcgggaat ccggccacgg atcaggtagc
     6181 ccatgagcac gggaatcaag gtcaccgaca gtccggccgc cgctgccatg gcataggtct
     6241 tggtaaacgc caacggagag aacaagcggc cctcctgcgc ctccagcgta aacaccggga
     6301 taaacgacaa ggtgatgatc agcagcgaga agaacagggc cggcccgact tcggcggccg
     6361 cgtcgccgat gacccgccag cgcgtctcgc cttgcagcgt ttcaccagga tgatcgtgtc
     6421 cccaatgctc cagatgcttg tgggcgttct ctatcatgac cacggcggca tccaccatgg
     6481 caccgatcgc aatcgcgatg ccgcccagcg acatgatgtt cgcgttcacc ccctggtagt
     6541 gcatcacgat gaaggcgacc agaatgccca gcggcaacga aatgatggcg acgaaagccg
     6601 agcgcaggtg aaacaggaag atgaaacaca cgaccgcgac gaccaggaat tcctccatca
     6661 gcttgtacgt gaggttgtcc acggcccgtt cgatcagtcc cgagcggtcg taagtcggca
     6721 cgatctccac ccccttgggt aggctggcct gcaaggtctg aagcttggcc ttgacggcgg
     6781 cgatggtttc cagggcattc ttgccggaac gcatgatgat cacgccgccg gcagcttccc
     6841 cttctccgtc gagctcacca atgccgcggc gcatttcagg gccaatctgg atgcgtgcca
     6901 cgtcacccag gcgcaccgaa acgccgccag gcgtcgttat tagcggtaca gcacgaaaat
     6961 cgtccaggct ttgcagatag cccgaggccc gcaccatgta ttcggcctcg ccaagctcca
     7021 gcaccgaacc gccggcttcc tggttggcct tctggatggc ttcgaccacc ttggtatgcg
     7081 gaatcgcata ggcggccagc ttgtctgggt cgagcacgat ctgatattgc cgaaccatgc
     7141 cgcctatgga tgcgacctcg gccacattgg gcacggtttt gagttcgtac ttgagaaacc
     7201 agtcctgcaa ggcgcgtagt tgcccggcgt cctgggtgcc gctgcggtct atcagggcgt
     7261 actggtaaat ccagcccaca ccggtggcgt ccggccccag cgaggctttg gctgccgccg
     7321 gcagacgcga ctggacctga ttcaggtact ccagcacgcg ggagcgagcc caatagaggt
     7381 ccgtaccgtc ttcaaacagg acgtagacaa atgagtcacc gaagaatgaa tacccgcgta
     7441 cggttttggc cccgggcacc gacagcatgg tcgtggtcag cgggtaggtg acctggtttt
     7501 cgacgatgcg cggcgcctga ccgggatagg tggtgcggat gatgacctgc acatcggaca
     7561 ggtcaggtag cgcgtccagc ggcgtccgca tgaccgagta cacgccccag gcgctgatca
     7621 tgagggtggc caacagcacc aaaaagcggt tggcgatgga ccagcgtatc agccgggcga
     7681 tcacttttta ctccctgtgg ccgccggtgt ggcagcagac ggtgcacttg agggcgagat
     7741 ccgggttaac tgcggtagtc cctccgcgtc catgtagaac tcaaaactca cctggtcgcc
     7801 ggctgccagg ttgcgcggca ggtctttggc gggcggcgat ttgaactcca tggtcatggc
     7861 tccccacttg agcgagggaa tcggcccatg tgatagcgtg atcgcgtcct tgccgatggc
     7921 ttcgatcttg gcttcgcctt catgacgcgt cgcggtattg gctgcgactg gcttgggcgc
     7981 ctcattgagt cgcgcttcaa tccctttgag gctggcttcc gagtcgatca gaaactgcga
     8041 tgacaccacc acacgctgcc ccgcttgcag gccacgtttg atttcagtct ggccaccgct
     8101 ttcaatgccg gcttcaacat cgacgggccg gaatcggccg ttctcctcgg ccagcatcac
     8161 caccgtgcgt ttgcccgtct ggatcacggc ttcggtgggg atcagcaagg ccttctcggc
     8221 ccgcatgtcc atgaactgca tggacacaaa catccctggc accaggtgtg agcctgggtt
     8281 ggccagctcc agccgcgcct tgagcgtgcg cgtcgccggg ttgacctcag gcaggatgga
     8341 ctggaccttg ccgtcaaagg tggtgccagg cgctgccggg ctgcgcgcct gcactttcgc
     8401 ccctggtcgc agcaaggccg cctgactctc cggtacttct gcgttggccc acacagtgga
     8461 caaaccgttg atgcggaaca tggtggcgcc gggcatcacg gtcatgcctt cgcgtgccag
     8521 caactcgacc accaccccgc caatcggtgc tgtcagtgtg atgcgggctt gcgtcttgcc
     8581 actggcctca acccggccaa tctgttcgtc gctcatgcct gcctggcgca tgcgctgccg
     8641 ggcaccgtca accagcgagg ccagatcatt gcccttcata cgcttgatgg cgagaaactc
     8701 ctcctgcgct gcaacccagt ctggaacgta caggtccacc agcggttgtc ccttggagac
     8761 gcggtccagt gtggcacgca cgtacaggcg ctcgacatag ccggtcgctc tggcctggac
     8821 aatggcttgg tcgcgttcgt taaatgcgat gctgccgacc gcagagactt gaggcgacag
     8881 cgtgccctca gtgacggctg cggtacgcac gcccaggttc tgctggacgc gcgggctgac
     8941 agtgaccttg ccctggtcgg aatcgccatc ggcatagacc ggcaccagca tcatgtccat
     9001 aaaaggcgac ttacccggtt tgtcgaactt gttccccggc accatggggt cctggtagta
     9061 caggatcttg cggccgctga ccggatcggt atcgccggcc ttgaggccag cgctgatgtg
     9121 gcggcgggtg gcgtcttcgc cttcggcgat gctttggggt ccggctgtgg acgcggccgc
     9181 tggactcgac ggtgcgccac ccggtggggc ggctgcagcc atgctgctgc cgtttttcat
     9241 gcccagggcg tacaggccat aacctgcggc gccgagaacg ccggcggtga tcagcgcggt
     9301 tataaggtat ttcttgttca tggctgttcc ttggctggtg ccggcatgtt gtgttcgggg
     9361 acgaggaagt taagctgggc ccaggaccgc gcggtttcca gttctagcgt cagggcttgc
     9421 attcgggtgt caatctcatc acggcgtgcg gccaatgcca cagccaggtc gctcttgccg
     9481 gtgcgatagg ccgtgagcgt ggcctcggtc cgctgccgtg cggtgggaat cagctcactg
     9541 cggtaacggg ctacccggtc cttacccgtt tgccagtcgt tcagccagcc acgcacctcg
     9601 gcttcatggc tgcgcagcat gtcctcgtag cgcgctttgg cttcgtcaac catggccagc
     9661 ttcgcggcca gttcacggtt ttggcggttc ttctggtccc actggagggg gatggagacc
     9721 ccgatggaaa ccatgttgga gtaagccgaa ccccgctgag cgtagctggc ctcgacgctc
     9781 cagtcggctt gtttgttggc ttgtgccagc ttggcatcgg tttccacggc atcgacttct
     9841 gcgctcaggg ccagcagatc cggatgctgc ttgaggtgct cgctcgacac ttttccctgc
     9901 agcggtgtgg tttgccaggg cggtgaacct gaaaacggtc gctctgcgtc tggagcacct
     9961 atccagcggg cgagcgcaat gccagcaccg cgcgcctgcc ggtcgatttg gctgaggcgg
    10021 tcttccagtc cgatgacagc ggcgcgggcg gcaaacacat ccgcctggct gccccggccg
    10081 gtacgaaagg cgctatcggc cgcttgtacc tgcagccggg tttcctcgat ttgctggagc
    10141 aacagttcac gctgggcttg ggtgtagtaa cggtccagcc aggccagcgc cgtgtcgcgc
    10201 tgtaggttcg ccagtgtcag ttgccgctgc gcctgcaccc gctgtgcgtc gcgttcaaag
    10261 cgttccgacc gcagttggcg tttctcggag cgggtgattt cctgcgaaag accaatgcgg
    10321 cgctgcgtca tgaagtcgcg cgtcaggctg ccgcggtccg gaccattggc cggcaggttg
    10381 tcgatgccga atttgagcac cgggtccggc aactggtcgg ccgccacggc ctgttcacga
    10441 gcggcggtgg tcagcgcgcc ttgggcgacc aattgctgag accggccgac ggcgatgcgc
    10501 tgcgcctcaa tcagggtcaa agggtcaatg gcccatgacg cggacgccat ggccaccacc
    10561 aaggcggcag cggcagtccg ataaatcgga aagggaataa acataaaacc tccagagcac
    10621 aaagaatgac ctgtcgatgc acgaaaaatg catcaggcag gagctaagtg gtctgggagg
    10681 tcagaggcgg aagcagcagt tccggatggc tacagggggc gccgtggtgc gctgtaaatg
    10741 gggcgcctgc agcggcgctt ccaaaaatgg cggaatgacg atttgtagac tgaaatctgc
    10801 aggcaatgca tcaatggcta ccggcgacgg caattgatat ttgtcggcag actgctgtcc
    10861 cgcctggcag tgcgcgtggc acagaccagg ctgctcgtcg tccatgttta gtgccatgga
    10921 ttcggcgcag ggcatgcccg cctcggccat ggcagcgact tcggcgacct ttgtcccgct
    10981 gcctggacag gcgtagccag ccaccgccag ctgcataaac agcaggctga ccagtgcaaa
    11041 aagcacagtg atcaggcggg tgcggcggtt ccgggtcatg gcttgagtgt aatgaatctg
    11101 tgatctcaag cgctgaaaaa agttctcagg cgcaacagcc ggacccaagg cctttatcct
    11161 ggatggaggg acatggcatc gttccgtagg aacaaaacac gcagcaatcc ccctgtttcg
    11221 gacgcagcac cgtgtggcat tgctcgcact cgtaaaacca tt
//
LOCUS       DAIDFG010000072        11230 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_72_length_11230_cov_17.592125, whole genome shotgun sequence.
ACCESSION   DAIDFG010000072 DAIDFG010000000
VERSION     DAIDFG010000072.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 11230)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11230)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11230
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72_length_11230_cov_17.592125"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1945 a         3615 c         3738 g         1932 t
ORIGIN      
        1 aaacgtgtca gcgaactcgc cagccagtcg gtcaaacgcg tggcgctgga actcggcggc
       61 aagtcggcca gcgtcatcct cgacgatgcc gacatggaag ccgcggtcaa gggaacgatc
      121 tcggcctgca tgctcaacag cggccagacc tgctcggccc acacccgcat gctggtaccg
      181 gccggccgtt atgacgaggt caaggcgctg gcgaaaaccg tgatcgccag gttcagcgtt
      241 ggcccgagcc ttgaggaaac cagcaagctc ggaccgctgg tcagcgcgaa ccagcgcgac
      301 cgtgtgctgg ggttcatccg caccggcatc gaagagggcg cggacgtgat tgccggcggt
      361 gaccagccgc ccgcctttga gaagggctac tttgtgcagc ccaccatcct gggcatcaaa
      421 gctacagaca ccctggccca ggaagaaatt ttcggccccg tgctggtggt gattccgtac
      481 aaggacgagg aagaagccgt ggcgattgcc aacagcacca tttacggtct gggcggcggc
      541 gtctggagcg gcagcgacga acatgcgatg aaagtcgcac gccgcatccg caccgggcag
      601 gtcgacatca acggcggccc cttcaatgcc aatgcacctt tcggcggcta caagcagtcg
      661 ggcaacggcc gcgaggcggg tgtgtacggg tttgaggagt ttctggagct caagtcgctg
      721 cagctcaagc cggtcgcggc cgcctgatcg cctgcacctg atggtggggg cctgattcgg
      781 ttaggctatc gcccacaaca agcaggaggc gatcatggtc tgggtcaaac ggtttttctg
      841 gggggtactg gttctggtcg cgctgctggc agctgcgctg gctgtgtacg ccgcacgggc
      901 ctttccggcg ctggacggcg aactcaagct gcccgccctg gcagcgccgg tgaccgtggc
      961 gcgcgacggc gccgacgtca cccacatcaa ggcccagtcc ccgcgcgacg cgtggatggc
     1021 gctcggttat gtgcatgcgc aggagcgtgg ctggcaactc gagttcaatc gccgcgtcat
     1081 gcacggcgaa ctctccgagg tgctgggcga ggccacgctg gacaccgaca agctcatgcg
     1141 cacgctgggc atcatggaag cggccgcgcg ccagtggcag gtgttgccgg ccgacggcaa
     1201 ggcggcgctg caggcctaca gcgacggcat caacgccttt tacgccggca gcgcgcaggc
     1261 gctgtcgccg gaattccaca tccttggcgt caagccgggc ggtcgctcgg gccggccctg
     1321 gacaccgcag gacagcgtcg gctggtcact gatgatggcg ctggatctgg gcggcaactg
     1381 gggcaccgaa tttgcacgcc tgtccgcggc caaagtactg acgaccgaac agctgtggca
     1441 gctgttcacg ccttacccgg gcgaggcacc ggcttccaga accgacttcg ccaagctgta
     1501 cgccgggctc ggcatttaca aaaccgtgcc ggatgctatt aaaacaggag ctgctggcgc
     1561 aggtatggaa agggctgaag cccgatttga cgcaaaaact cagcaaggga tgcgggatct
     1621 gatggcgcag gacatcaatg gctgggcgag cgacatcggc aacgtcgaag gcaagggctc
     1681 caacaactgg gtgcttcccg gcagccgcac acgcagcggc aaacccctgc tggcgaacga
     1741 cccgcacctg ggcctgtcgg cgccggccat ctggtacttt gcgcacctgc gggcacccgc
     1801 cggcaaggcc gcggatggaa gcacgctgcc gggcatggac gtgatcggtg ccaccttgcc
     1861 cggcctgccg tttgtggtgc tgggccgcac cgacaaggtc gcctggggct ttaccaacac
     1921 cggtcccgac gtgcaggacc tttacctgga gcagatcaac cccgccaatc cgcagcagta
     1981 ccgggtgccg ggggccgcgg gccggacgtc atgggccgag ttcaagacac gccttgaaac
     2041 catcgccgtc aagggcaagc cggacgtcag cctgacggtg cgtgccagcc gccacggacc
     2101 ggtgctcagc gacgtccaaa aagcgcatgg cgaggtgctg gacaccggca agtacgtgct
     2161 tgcgctgcgc tggagcgccc tcgagacgga caacctgacc gtggtggccg gcatgcgtgg
     2221 caaccaggcg cagtccgtgg acgacctgct ggctgcctac gcgaattacc actcgcccat
     2281 gcagaacctc gtgatggccg atgtggccgg caaggtggcc tacaaggccg tgggcaaggt
     2341 acccctgcgt cagcccgaca acgacatacg gggtattgca ccttcaccgg gctgggaggc
     2401 gaagtacgac tgggccggct ggttgccgta tgcccagacg ccgcagaccg acgcggtggc
     2461 catcggcaag aaaggctggt ttgccaatgc caaccagagc gtgaccccgc ccggttatga
     2521 cttcttcatg ggccaggact gggtggctcc ttaccgcttt gaccgtatcg agaagctgct
     2581 gggtgacacc ccccggcatg atctggcgtc catgcagaag atccagggcg accccttgtc
     2641 gttggccacg ctgacgctgc tgccgttgtt gcagaagacc gcatctcaac atgtgctggc
     2701 ccagcaggcg caagccgtcc tggccggctt cgacggccgc atgcgcgccg acagcgcggc
     2761 cccgctgatt ttctcggtct gggccgatga actcacgcgt ggcgtgatag gcgccaggct
     2821 gggcgaagag cgtttcaagg ccatgtatgg caagcgccat tttcgcagtg ccatcgagga
     2881 gattgtccag cgcaacgacg ccttctggtg tggcgccgca ggttgcgctg cccagtccag
     2941 cgccgcgctg gaccgtgcgc tcacgcgcct gcaggccagc catggccagg taccggccca
     3001 atggaagtgg ggcgatgtcc actatgcgct gtcggtgcac cggcccttcg gcagcgtgcc
     3061 ggcgctggcc cgggtgttcg acgtgcgggt gccgacggac ggggactcct tcaccatcaa
     3121 tgtcggccag tactggccca acgacgccaa aatcccgttt gccaaccggc acgcggcgtc
     3181 gctgcgcgcc gtgtacgacc tggccgatct ggagaactcg cagttcatct accagaccgg
     3241 gcagagcggc ctggtgtttt caagccgcta ccgcgacatg cgccagacct gggcggcggt
     3301 gggttaccgc ccgctgcaaa tgaagccggc ggcgtttgtc cacgagcttc agctcaagcc
     3361 ctgacccgtg ccggcttcat gcggggacag cgtccacgtt gagccggtcc caatggcggt
     3421 ggcgggccat ggcggcgatc agggcctgca aaatctggcc gtcggccgcc ttgcgcgagt
     3481 cgcccagcag aatgcccgcg gtgggctcca tcagcgcgcc gtccctggtg tcgagcgcaa
     3541 aacccagcgt accgagcaac tgcgcgcctt cgttgagggc gcagacggtc ttgcagtgtt
     3601 tgtaggcctc cagcacgaaa tggaccgcgt cgcccagctg tgccagcgtg gcggcactgg
     3661 ccgcgccacc cgggatcacg accgcgtcga acatgatgga cgaggtgttg gaaaacgtgt
     3721 ggtccgcggg aagctgcttg cctgacgaag tggtgacggg gccgagctgg ggcgcgacca
     3781 ccttgcactg ggcgccggcg tccagcagct cctgctgcag gcgcttgaag ctcagcaggt
     3841 ccacaccgtc ggccaccagg atggcgacct tgcgtgtccg gatgttgtcg gtggccggcc
     3901 ggccggccat gctcagcgcc gcatcttcat cgacggtctg cgcaatccgg tagtcgcgaa
     3961 agcccagtcg cccggccgcg gccttggcgt ctggcgcgtt gatgcccagc ggcgcagcca
     4021 cccgggcggc cagcttggca tccacatgcg ccaggttgtc gaccatgcgc tggcggatgt
     4081 cgggcacctc gaccttggac agttcaaacc ggaaggcggc cacgatgtgg tctttttcgg
     4141 ccgggctctg gctgttccag aacagcgtgg catgggaaaa atggtcgtcg aacgaagggc
     4201 tgcgcctgcg gatttttgga gactccagcg cctgcgggtg ggtgtgaaag ccctgggcgc
     4261 cgccatcgac acgaaattcc gcgcctgtcg ccagggtgtt gggttcgtaa gcgacccggc
     4321 ccttgctgat gccctggcgg tgcatgccgt cgcgctggaa attgtggaaa gggcagaccg
     4381 ccctgttgat cgggatctcg tgaaagttag ccccgcccag gcgtgacagc tgggtgtccg
     4441 tgtaggaaaa caggcgccct tgcagcagcg ggtcatcggt gaagtcgatg cccggcacca
     4501 gatggccggg gtgaaacgcc acctgttcgg tctcggcaaa gaagttgtcg gggttgcggt
     4561 tgagcaccat cttgccgatg agctgcaccg gcaccaccga ctcggggatc agcttggtcg
     4621 ggtccaggat gtcaaagccg agcttgtctt cctggccctc ctcgatgatc tgcacgccga
     4681 gctcccactc cggaaaatcg cccctgtcga tggcttccca gaggtcgcgg cgatggtagt
     4741 ccgcgtcttt gccggcaatt ttctgggctt cgtcccaggc caggccatgc acgccgagtt
     4801 tgggcttcca gtggaatttc acaaaatgcg cgtcgccgtg agcattgatg aaacggaagg
     4861 tgtgcacgcc aaaaccttcc atcatgcggt agctgcgcgg aatggcgcgg tccgacatcg
     4921 cccacatcag catgtgggtc gattcgggca tcagcgagac aaagtcccag aaggtgtcgt
     4981 gcgcgctcgc ggcctgcggc atctcgttgt ggggctcggg cttgagggca tggacgaggt
     5041 cggggaactt gatcgcgtcc tggatgaaga agaccgggat gttgttgccc accaggtcgt
     5101 agttgccttc gtcggtgtag aacttgacgg caaaaccacg cacgtcgcgc accgtgtctg
     5161 ccgatccccg tgagccggcg acggtggaaa agcgcacgaa caccggggtt tccagctcgg
     5221 gattctgcag aaaaccggcg caggtgaact gcgtcatcgg cttgtagagc ttgaaaaaac
     5281 cgtgcgccgc ggagccgcgt gcatgcacgg cgcgttcggg aatgcgttcg tggtcgaagt
     5341 gggtgatttt ttcgcggagg atgaagtcct ccatcagcgt cggaccgcgc acgccggcct
     5401 tgagcgaatt gtggttgtcc gggatgtgca gaccctggtt ggtcgtcagc gtgctggcgg
     5461 cgtcggcagt aaaagcctga agctgttttt gcttttcggt ggccccgttg gaaagcttgg
     5521 gggcgctggc tgtactgcgc ttgggttgcg tcatctgggt tccttggaaa cggttcaaaa
     5581 aaaaggaggg gagtgccggc cggacaggcc gcccgtggcg gctgcagggg tgctagctgg
     5641 actcgcagtc gagcaggcgc tccagcccct tggcgacaag catcacggcc aggatcgcca
     5701 ccacggcgac catcagccag gccataacgg tttgcagcgg ttcggccgtg ccttcgggca
     5761 acatcgccag cagggcgccc agagcgacca gggccgccac cagccaggcc gcgggtccga
     5821 aaggggaatg ccgctggcgc gtgcccgctg tgccgggggc gtcggtgggg gaggatgttg
     5881 cgagtgccat cggatgtcct catgggaagg gggcggtgga agaaaccgcg gcggaaaaca
     5941 cggagggaaa cactggaggg agtagcagtc ccaccttact gggcggggcg gccaggggtc
     6001 gtagttgccg ggcgccagtg gctgtaaggt cagtcgcgag cccttgtaag acctgtctta
     6061 cttcgatgcg tcgcactgcc tgaccgccgc gtgggccgca caaacgttgt gcgaggcccc
     6121 gccgcaggcg cggccgcgcc ggggcttcct cctacagggc gacagtgggc tttcccgcat
     6181 gccgggtcag ccaggccttt taaggttgga ggactgcgag aaaagcacgc ggcccggttg
     6241 gggtccgcca ttttttttgc tgaaccggaa ctgacccgga gaaagcgtcc tgatgatcac
     6301 gccccgcatt gaagaagaac gcacactgcc agccccgcca tcgcggccgc tgaatgagga
     6361 cgtgctgcag tcggccgaac aggcggtgct gagcaacccc gcttcggacg ggattctggc
     6421 ccctgccgac catgacgcgg cagcaccggg tgtgctgaca cgctaccgcg ggtcgctggc
     6481 cagccaggtg gcccgcaaac caggccagtc cgcgctgctc gccctggcgg cgggtgccct
     6541 gaccgccgcg ctgttgcggt ccctggtcag ccgccgcagg cgccacccac ggtcatgact
     6601 cttcatgcca cccctcccgg ctggagggcc cacgtgccgc ccggcagtcc gccaccggtg
     6661 atgccgccgt caccggagct accgcccgag gtgatcgact tgccgcccgg cgagcccgtc
     6721 cagccggtgc gcgagcctgg ccaggtgaat ccggctcagg ccttcgccgg tcgcgacgac
     6781 aggtcgcgga tggcgtttcg cgcctcatcc ccttcgggaa tcggctcgcc gctgtcacgc
     6841 cactgcaccg cggccttgaa accttcggcc tgggcataac ggcgaaacca catgccctcg
     6901 gggttgtggc gcgtgatgcc gtcgaacacg gtggccatca tctgcgtctg ctccagcccc
     6961 atggtcagca tcacctggtt gaccaccatc ttgtgcatgg ccagatgtga acgcggcacg
     7021 ccggcgatgc gctgcgcgag ctttagggtg gccgcttcca gctgatcggg cggcacactt
     7081 tcgttggcca gaccccagtc ggcggctttt tttccgtcga tggtgtcgcc ggtgaacatc
     7141 agctgcttgg cacgggtggc gcccagccgg taggtccaca tcgccgtggt ggggcagccc
     7201 cagacgcggg tcggcatgta gccgatgcgt gcatcgtcgg ccattaccag caagtcgcag
     7261 cacagcgcaa tatcgctgcc gccggccgct gcgtaaccgt ggaccttggc aatcgtcggt
     7321 ttcggactgc gccacaggct catgaaatct tcggtgttgc gcttcatgta ggcgtagtcc
     7381 accatcgggt cccaggggaa ctgctcctgc tggcaggggt ggtcgatgtt gccttcgccg
     7441 aagtgattca ggtcgtaccc gccacaaaac cccttgcccg cgccttcgac aatgatcacg
     7501 tggacctcat cctcggccgc agcccagtcg atggcggccc ggatctcgcg cggcatggcg
     7561 tcgttgatgg cgttgagccg ctcgggacgg ttgagcagca ggcgtgcgat gcgcgggtgg
     7621 gcggcgtcct tggctatgcg cagggtgctg aattcgggca tgtcttgtct ccggtgttgt
     7681 gatgaaaacc atcatagcca agggtgggac gggcaccatg acgggcgggg gttgtcaatt
     7741 ctccaatctg gttgcgcctg caaccagttc gccttatagt tgtgtccgca accaaataag
     7801 tacctgccca tgacttccat gaattccccg ttcccggacc gtgttccggt gctgattgcc
     7861 ggcggcggac cggtcggtct caccctggcc gctttgcttt cgcgttacgg catcgactgc
     7921 ctggtggtgg aagccgatga ggcttattgc agcggcagcc gcgccatctg catgtcgcgc
     7981 cgctcgcagg aaatcctcgg ctgggtcgct gccgacaagc cgctggtgga caagggcctg
     8041 tcctgggtcg gcgggcgcag ctactggcgc gacgccgagg tgctgcattt ccagatgccc
     8101 agcgagccga cccagcgttt tgcgcccatg gtcaacatcc agcagtttta tgtggaggag
     8161 tttgcgcacc gcgcaggcac cgccgccatg gacgtgcgct ggtccagccg cgtgacggcc
     8221 gtacgcaatg ttgccgatgg cgccgagatc gaggtgcagg gcccggcggg cccgcatacc
     8281 gtgcacgccg actgggtggt ggcttgcgac ggcgggcgca gcactgtgcg cgagcagttg
     8341 ggcctgcagt tgcagggcac gcagtacgac ggcaaatacg tgatcgtcga catcgtgcaa
     8401 aaaacaaaaa gggcggtcga acgcctggcc tggttcgacc cgccgtccaa ccccggctcc
     8461 accatcctga tgcaccgcca gcctgacgat gtctggcgca tcgactacca gatccgcgac
     8521 gacgaagacc cggtcgaggc ggtcaaaccc gaaaacgtct tgccgcgtgt gcaaagccat
     8581 ttgcgcatga tcggcgagga cgaaccctgg gagccgctgt ggatctcgat ctacaacgcc
     8641 aaatgcctga gcctggacag ctaccgccac ggccgcgtgt tgttcgccgg tgacgcggcg
     8701 cacctggtgc ccatcttcgg cgtgcgcggg ctcaactccg ggctggacga tgccggcaac
     8761 ctggcctgga agctggccgc cgtgatcgag ggcagggcgg gcgagaacct gctcgattcg
     8821 tattccaccg agcgggtcca tgccgcgcgc gagaacatcg cctatggcgc caaaagcacc
     8881 gagttcatgg cgccgcccaa ctttgcgttc aagctgatgc gtgaagccac cttgcggctg
     8941 gccctcagcg acgacaaggt gcggccgctg atcaacccgc gccagtcctc gcccatcacc
     9001 tatgtcgact caccgctcaa cgtaccgcaa gccggtgcgt gggccagcga cttggccgca
     9061 cccggcgcgc ccgcgcctga ggccctgctg gccggcccgc acggcccgct gcacctcacg
     9121 caatgttttg gccgcgacat ggtgtgcctg gtgttcgccg acggcgcttt gcccgatgcg
     9181 ctgcgggact tgccggacca cggcgtggcg gtgctggaga ttgagcccga ggccgacacc
     9241 ctgggccagg cgcgccagcg ttacggcctg cccgatgcca gcgcagaggc cctggtgctg
     9301 gtacgccccg acggttatgt gatgggccgc tggcgcggcc tggacccggc gccgctgctg
     9361 gccgctttgg aactcaaagg attcgctgta tgaccgatgc cgacctcgac cggagttatt
     9421 ccgccctgtg cgaagccctt gcgcaggcgg gcgaggccag ggcgccgctc ctgctggcga
     9481 tgctgagcct gtcgctgatg agccgcttcg agcgcgccga ccaggtcttg cccttgatcg
     9541 ccaacgcgca gacacagtgc gccgccgggg ccgctgatgc cgcataaatc ccccggcatc
     9601 ccgcggctgg acgagttcct gacctaccgg ctgcacctgg tcaacaagct gaccgacaag
     9661 ttcagcagtg atgcctatgc cgacgagttc aacctgccgg tcggcgaggc gcgctgcctg
     9721 gcggccatcg gcaactttgc gccgctctcg gtggtggaac tcgcggccaa ggccaacctc
     9781 aacaagggcc aggccagccg tgcggcgcag tcgctggtgg agcgcggcct ggtgtccaag
     9841 gaggccagta cctccgacgg ccgcggcgtg gtgttgacgc tgaccagggc aggccagcgc
     9901 ttgtggagcc gcgtgatggc actgattgcg cggcgcaacg aagagatttt tggctgcctg
     9961 tcggcaacgc agcagcacca gctgggccgc atgctggacc gcctgattgc ccatgcccgg
    10021 gcctgaatcg ggcatttaaa cgccgaaacg ggttgatttg ggttgattcg gggcgcgaag
    10081 tacccatgtg aggagcacta gcgggcttaa aaccttaaaa taaaaggttg ccctttcagg
    10141 tcctctttag gcccccatga acgcccccat cgacgtctct tttttcgcgc gcgccgccaa
    10201 acccctgacc agctaccgca agtactgggc cagccgcttc ggcacgtcga aattcctgcc
    10261 catgagccgg gccgagatgg accagctcgg ctgggacagc tgcgacatca tcatcgtcac
    10321 cggcgatgcc tatgtggacc acccgagctt cggcatggcg gtcatcggcc gcatgctcga
    10381 agcgcagggc tttcgcgtcg gcatcatcgc gcagcctgac tggcaaagcg ccgacccatt
    10441 caaggccctg ggcaaaccga acctgttttt cggcgtgacc gccggcaaca tggattcgat
    10501 gatcaaccgc tacacggcgg accgcaagat ccgcagcgac gacgcctaca cccccggcga
    10561 catcggcggc aaacggcccg accgcgccgc catcgtctat tcgcagcgct gccgcgaagc
    10621 gtacaaggac gtgcccatca tcctgggcgg tatcgaaggc tcgctgcgcc gcattgccca
    10681 ttacgactac tggtccgaca aggtgcgccg ctccatcgcg gtggatgcca agtgcgacct
    10741 gctgctgtac ggcaacgccg aacgcgcaat tgtggaaatt gcccaccgca tcgccgccaa
    10801 ggagccggtc gcgaccatga ccgacatccg cggcacggcc ttcatccgcc gcacgggtga
    10861 cgagaccgcc cagggctggt tcgagatcga ctccaccagc gtggacgcgc cgggccgggt
    10921 cgaggcgcac gtcaaccctt acctgatgat ttcggagcag gccaaggagc agggcgcaag
    10981 ttgcgcacgt gaggacgaag cggctgaggt tgcggcacag gcggaatcgg caggaagcac
    11041 cgccgtccac gcaaccgctc gccctgagcc tgtcgaagga gctcccaagg cttcgacaag
    11101 ctcagcccga acggacatgg gctccaccat caaaccgctg accttcgtgc ccaaccccgc
    11161 tttgtcgcac atgcagggca ggatcaaggt gccgccgcgc gaccgctcgg tgatccgcct
    11221 gccgtcctac
//
LOCUS       DAIDFG010000073        11154 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_73_length_11154_cov_18.755654, whole genome shotgun sequence.
ACCESSION   DAIDFG010000073 DAIDFG010000000
VERSION     DAIDFG010000073.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 11154)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11154)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11154
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73_length_11154_cov_18.755654"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2088 a         3516 c         3667 g         1883 t
ORIGIN      
        1 tcaacgcagc aggccgcccg agcccggctg atcgcgcgcc gcaggggact tgttcggcgc
       61 ttccctaagc gccattgcaa gcgggcgcgc ctgatgcggg tcaagccgct cagcgcgccg
      121 cggcgtgaca tgaggtcaaa cagggcctca cgccaatgcg ccatgccggc gccacccgtc
      181 ccaaagcacc atcccacaaa tggaggatgc ggcaacaagc gagtccggcg aaaattgacc
      241 aggaatactt tttctgggga cctccatgca aatcctggtc gtggcaatca gctccagcct
      301 ccgtcttctt gggtcaggcc tccttgcatt cgcaattgtt ggctgcagca ccatcacgca
      361 caagcaggca gtgggtatac caaaagacga taagccccag catattgcga tcttcttcga
      421 cggcacgcac aacgatgaag cgtcggatac aaatatcaaa cgactgcact cactggtgag
      481 cttgcaaggg cgcgcggacc tcactacctt gtacatcgaa ggcgttggga cacgcgccga
      541 cattgcgggc atggccactg gctacgggct cggtgagcgg gttcgcatcg cctatggttt
      601 tcttttaaag caccaccgcc cgggagacaa ggtgtacctg tttggcttca gccgcggggc
      661 gtatgccgcg cgggtactga actcgctgct gtaccacgct ggcttgcccg acaaacccaa
      721 caaccggacc cacgtggacg tggcggaaga ggtctacgag ctggtcaagg tcgagagcga
      781 ctaccctgcc cagtacgagc cattgcggcg tcaacgtgtg caagaagatc ttcagacacg
      841 aaagttgggg aaagtggatc ctcggacacg caagttgggt ccggcgccgg ctgtggaagt
      901 gcaagtgctt ggactttggg acaccgtgga agcacttggc gcacccaact ggctgagccg
      961 gatcagccat aaagcccaga ttcagcagca cctcgcggac gtcgataacg cgaattcgcg
     1021 gtacggcgac aagctgtgca atgttcacta tgcgtttcac gccatgtcaa ttgatgacaa
     1081 tcgcgaatgg atttttaccc cgcagctcct gacgcgccgg caccttgtgg ccggatgttc
     1141 acccgccgag cgccctccca ttctcgacga gcgcggcaac gtgcggcctg gtcgacttcg
     1201 cgaggtgtgg tttgcgggtg cccattctga cgtgggtggt gggtacgcag acgcacgcat
     1261 gagcggggtg agcttgaact ggatgcttga ggaaatgcgc aaggccaacg tggatctggt
     1321 gccaacagga accaaagtgc cggaggatcg ctttggcaca agtcacaacc ccgaggccgg
     1381 ggctttctcg gccatctatc attcgatcaa ccgcgacctg gtggcgtacg tgcgcagcga
     1441 cgccagcctg gtgaaccggc tgtgcgtgca cccatcggtg tttgaaaggc ggaaatttgt
     1501 tcccgtgaga tcgcatgaaa acagtcagtt gcagttgcga aagcctggcc gcgcatgcct
     1561 gaagatggcg aagccgcaac tcaacagcct ggacgcgcac ggccgactca cacaatctgt
     1621 cgaaacggga gatcaatgcc cgacaggcac cgcggccatc gtggacgtac gtgaagatcc
     1681 agattgccag gggaaatagc catgaaagtt cgcaatatct tgctccccgc ggtcctggta
     1741 gttaccgcga ccctgaccgg ctgtgctgcg gttcggtatg aaccgacgtc ctatgactca
     1801 cccaagcccg tcgaggccgg ggtgccgggc tgcttgtcgg cgggcgccct tgccgccccg
     1861 ggcgatgagt gcctcgcgct tgtacgcgcc gaccagtggt ggtccgacac aggcgtaact
     1921 gtgaagaaac aggcagccta caggctgacc gttcccccgc atcaagtctg gttcgacaag
     1981 ggcatcgtca ataaaccgcc ttacggtgac cccgggaacc gactcatgaa tctttttgag
     2041 agctacaagc gacatgaggg gggcttgtgg ttcagcctga ttgcggcggt cgtcgagcgg
     2101 ggtcctggcc tggacagacg ggaataccaa agcgtcgata tcggagcggc catcaagaaa
     2161 gagtgcgaag agtgcgacag cgttattttc gtggcgaagg aggacggcgc cctgtcgctc
     2221 taccccaatg atgcaaaagg acctgcctcg caccctactt ttttctacga gaacaacagc
     2281 ggtcagatct gggtgcgagt gcgctggcta acagataaag agaaagaggc gtcggcggcg
     2341 acagaaacgg gggtggccga tggctgcagg ccgcggacta agccgacggt cgcccaataa
     2401 cccagcggcg ggcgcctgtg tttccgtgtt gacgatcaaa aattccaggc ggcgagcgcc
     2461 tggcgtgatg cgtggcgcac gcaagctatc ggacatcgaa cattcccgat aaatagaaag
     2521 tcgccatgac aagttccgaa tgcgatatgg aacgttttag gccttcagcc ctttgatggc
     2581 gggcgcaacc agctatcaaa taaatagcat ctccgtacgc gctggatgga aactctgcca
     2641 tccctacccc gccggcacgt gcactggctg gcccatctgg tccttcagcg cgacacccga
     2701 gcgttgttga acacgcatct ggttcagcag ccaggcgatc ttggccgctg cggcctgcgg
     2761 ggtcaggccc tgggtccgga tgttggagac gcagttgcgg tcggcgtcgc tggtggctgc
     2821 gccgggcgcc caggtgatgt aggcgcccag gctgtcgggc gacgacaggc ccggcctttc
     2881 accgatcagc accagcaccg cctcggcctg cagggcgcgc gccacggcat cgcccgccgc
     2941 aacacggccg tggtgcagca gcaccactgg tgccagcacc cagccctgcg tttgcagcag
     3001 cggcagcagc gcttcaagca ccggcagggc atggcactgc gtggcctggg cggacaggcc
     3061 gtcggccagc accacgacca agccgccgcg caagccgctg gcttgcagca gcgccagcga
     3121 atcggcatcg agctggcgcc cgaggtcagg ccgcatcagg tagctgcgcc tgtcgggtgc
     3181 ggcgctgcgg cagaccagcg cgggctgacc cagcagcgac aggccggcca acaagggacc
     3241 ggtatccagc gcggcgccta ccgcatcacg cgcccgagcg tgggccaggc ggaagtccag
     3301 cagtgcgggt gtggccagcg aggcgccggt gcgcggcagg ccgatgcgtg cggccgtcag
     3361 cggccgcagc gccgcccaca actcggccgt gccggacggc gtatcgagag gtgccggggg
     3421 ctggggttgc ttcttgtcgt tcatggcttg gcgctgttgc ccagcagttg ccggaaaggc
     3481 agcggcacgg cgcccggccg cacgcggccc tggccgtcca ccaggccctg cgcggccagc
     3541 cacaactcga actcgggcgc gcaccgcttg cccagcaaat cgcgcatggc cagcgcgtcg
     3601 tggaaagaag tgctctggta acccagcatg atgtcgtcgg cgccgggcac acccatgaca
     3661 aagttgacac ccgaggccac cagcagggcc agcagcgcgt ccatgtcgtc ctggtcggcc
     3721 tccgcgtggt tcgtgtaaca gacgtcgcag cccatgggca ggccgagcag cttgccgcag
     3781 aactggtctt cgagcgcggc gcgcgtgatc tgtttgccgt cgtacagata ctcggggccg
     3841 atgaagccga ccaccgtgtt gacgagaaaa ggcttgaagt gccgcgccac ggcgtaggcg
     3901 cgcgcctcca tggtctgctg gtccacgccg tggtgctgcc cggccgacag cgcgctgccc
     3961 tgccctgtct cgaaatacat gacattgctg ccgaccgtgc cgcgcgccag cgactgcgcc
     4021 gcgtcgtgcg cctcctgcag cagcgcgaga ttgatgccga agctgcgatt cgcgccttcg
     4081 ctgccggcga tggactggaa caccaggtcc accggcgtgc cgcgggcgat caggtccagc
     4141 gtggtggtga catgcgcgag cacgcagctt tgcgtgggaa tggcgagttt gctgcgcact
     4201 tcgtcgagca ggcacagcag cgcgtgcgcg ctgtctgggc tgtcggtggc ggggttgatg
     4261 cctatcatcg cgtcgcccgc gcccagcagg aggccgtcga gaatgctggc ggcaatgccg
     4321 cgcaagtcat cggtcgggtg gttcggctgc agccgcaccg agaagcgccc gggcaggccg
     4381 acggtgttgc gaaaacgcgt gaccacgctg catttggcgg cgacgatcat cagatcctgc
     4441 aggcgcatga gtttgctgac ggcggccacc atctcgggcg tcaggccggg cgccagggcg
     4501 ctcaagacgg cggtgtcggc agtgtcactc agcagccagt cgcgcaggcc gcccaccgtg
     4561 agctgcgcca cgggcgcaaa ggcggcgctg tcatgcgtgt cgatgatgag ccgggtgacc
     4621 tcgtcggcct catacggcac gagggcgtgg ctgagaaagt ctttcagcgg cacgtcggcc
     4681 agcgcaaact gcgctgccac ccgctgctcg gccgaggtgg cggccagccc ggccagcacg
     4741 tcgccgctgc gcagcggcga ggcacacgcc atcaaggtgg cgaggtcgct gaagacatgg
     4801 ttctcttgac caaccggcat acggtacatc gctgtgggcc caggttaagc ctctatcgta
     4861 ggggaagcgg ccttgccgtg cggcagaacg cacggggctt cgccggcgcg caacgccgga
     4921 aaaacacgcc cgggtgaagt tcatgcatgt tttatgcctc gagcccttgc tgtacctgcg
     4981 caagcagcta ttaaatacat agcaaggcaa tacgcccttt gaggggcgag cagctggaac
     5041 tgccggagcg ggcggcggcg ttgcggcttc aggggcgcag gaatgggctg gcagcgctac
     5101 agaggtgctg attcacttgc gctctttgct ggcgcgggca tgggcctcca acccgccgcg
     5161 ggctttccat tccccataac cgccatgcag gatgcgcacg ttgtcgtagc cggccatcct
     5221 cagcgccatc gccacctgac ctgaaaacga accggtattg cagtaggcca ggatggtcct
     5281 gtccttcggc agcttgccgc gctcggcaaa caccttgcgc cattcgatgt tgcttgcgcc
     5341 cggaatgtgc tctttcgcga attgccccgc gtcgcgcacg tcgagaacgt agaacctctt
     5401 gtagtcctcg gccggaatct gttcggcagc gatggtgccg ctgttgtagt cggaaaattc
     5461 gaagtaggtc tccagcgctt cggtggtggc cttgtcgagc tgggcatgcg caacgaccgg
     5521 cagcgctgcc aacgcggcca aggcggcgga tgcggccagg cggcgcgaac agagggcgag
     5581 gaacttcagt gtcatggatg gtctccgggt tttgcaaaag cttgggggaa tcaatgcgtc
     5641 agtgcgcggg actgagccgg gggtgggttt cttcacccca gtgttggagc acagcacccg
     5701 acagcaacat cgccacggcg acgaaccaga aggcggtctc gatgcgcccg cccagggctg
     5761 cggccgcacc caggcccagc gcgcctatgc cgtaaccgag gtcacgccag aagcgataga
     5821 tgccaatggc cgatgcgcgc cagttggggt gggcgatgtc ggccaccgcc gcgctgaggt
     5881 tggggtacag catggccatg cccagaccgg ccatggcggc cgaggcgctc caccacgcag
     5941 aaccctggcc cagcggcaac aacgcgacgc cggcgccgca cagccacatg ccccagacat
     6001 tcaggcggtg ccggccgacg cggtcggaca acttgccggt gaagaactgc gccccgcccc
     6061 aggtgaagcc gtagacaccc acgatccagc cgatgccggg caggctgaca ccctgctggt
     6121 gcagatacac cggccagaac gcccagacca gggcatcaac aaacttttcg accatgcccg
     6181 cctggcacag cgcggccatg cggcggtcac cccaactcat gagggcaaac atttcccgcg
     6241 tggtcggctg ctcgctgacg cccgtcgggt agcgcggccg cagcacttgc gccgaagagg
     6301 ccgcagcgtg gcgcttgacc tcggcatggg cccagggcag cgtctccgcc acgcccagcc
     6361 aggccagcag cgtggccagg ccgatgacgg cagcaccaaa ccacaacaaa ccttcgcgcg
     6421 ggcccagcag cgaggcggca taacccgtca ccacaccggc gatggcgacg cccacataac
     6481 cggcaaactc gttgagtccg atgaccagtc cgcgctggtc ggcacgcgtg atgtcgagct
     6541 tggccgtctg tgtcatcgac caggtcaggc cctggttgac acccagcagg atggtcgcca
     6601 gcacaatcca gctccacgac gtggcgaagt agacgatcag cggaatcggc agcgccacca
     6661 gccagcccag caacagcaca cgccggcggc ccaggcgttc agccaggcga ccggcggcga
     6721 agttcatggc gcccttgacg aaaccgaagg cgacgacaaa ggccgccagc agcatgaaag
     6781 agccgcgcgg cacaccaaac tcggtttcgg ccagcgccgg aaccacgttg cgcatcatcc
     6841 cgatggtcat gccgacaagc agcacctgca gcagttgctg cagcacctgg ccgagattgg
     6901 cgcgaatgcc gaattccatg ttcattcagc tcaggcgtgc gccggctccg gtgctgttcc
     6961 acgcaggttg acagcgacga tgcgggccat gtcctgcggc tgcggtggaa tgtctgccgt
     7021 cagcgcatcg acgaagtcct cgcgcgacat ggcgagcatg gggttgaaac gtttctcgaa
     7081 acccagcgtc gatgcgggtt tgccggacag gccggcgccg caggcgctgc cggcctggtg
     7141 gccgggatag atttcgaggt cggcgggcag ggtcagcagc ttggcatgca gggtatcgtg
     7201 cagctggccc gccatttccc gctcgcggcc cgccaggtcg gggcgcccga cggcgccgac
     7261 aaacagggtg tcgccggtga tgacgaacca gggctcgtcg ccccggcgca ggtcgcgcac
     7321 cagcaggcag atgctgtctg gcgtgtgccc gggcgtatgc agcacatcca ccaccacgtt
     7381 gccagcctca agacgctggc cgtccgtgag gggcgcgaat tcgaacaaga cccgtccctg
     7441 gttgctctcg tgcaggtaat acggcgcgcc ggcccgcctg gccagctcgg ggccacccga
     7501 gtagtggtcg gcatgcacat gggtgtcgat gacgtgggcg atcctgacgc cggccttctc
     7561 ggcctcggcc atgaaccagt cctcgtcgcc ggcgaccacg tcaacggcca cggctttcgc
     7621 caggcccgcg cagccaaaaa aataggacag ggtggcattt tccgccggtc gttgttggaa
     7681 gaacatgttt gtctcctgaa tcagaacacc aggaccttgt cggcccaggc cgtccattgg
     7741 gtgagctcgt ccagggtgct gcggtgcgag ccggggagga ggtcctcgac gctgatgccg
     7801 cgcgcgtcca tgcaggtgcc gcagacgccg atcaggccgg cgtggctcac caccgagccg
     7861 agcatcactt cgaggttgta aaagccggta ggcactttct ggaggcgatg cgcggcagac
     7921 gcggcgtcac caatcagaaa aacccggacc tcgttgcccg gctgcctggc cagtgcgccg
     7981 gccaggcgcg ccccgttgta cgtgcgctca ctgccgtagg gcgcgtcgtt caggatgagc
     8041 agtgtgttca tgccggtgct tcagcttgtc tgttcgatgg gcaggccacg ggcgcgccat
     8101 tcggccacgc cgtcggtcag gtgaaaagcg cggtagccct gcttgcgcag caactcgacg
     8161 gcgtctctgg ccatcaggca aaacgggccg cggcaatagg cgacaacggg aacatccttg
     8221 gggagctctt tcagccgctt cctgagctcg tccaccggca gtgagcgcgc gtgcggcaaa
     8281 tgggcgctgg cgaattcctc ttccggccgc acgtccagca ccaggacctc accggacttg
     8341 gcctttttca tgatggccgc ccggtcgatg ccctcgagtt catcgccgtg ctcgacgatc
     8401 gaggccaacg cgacctgcaa ctcgaccagc cgctcctcgg cttcggcgcg caagttcacc
     8461 cagaggtcag ccacgctggt gctggccagg cggtacagga cgtacttgcc ttccttgcgc
     8521 gtatcgacga gtcgcgccat gcgcaactgt ttcaggtggg cgctcgcaag cttgacgctg
     8581 atttcagcct gcgccgcgag cacttcgacg gtcttctcac cctggcaaag caactcgatc
     8641 agctccaggc gcttggggct ggcgacggcc ttgccgatgc gggcgacttg ctcgtaaaga
     8701 tggtccttga tgtgtctcat gccatcaatc taacgtttgt tagattgttt gtcaacctcc
     8761 gacggctgac tcgcgggagg aaagcctcag agccatcggc gcacccgtgt ctggtaggcg
     8821 gcatagtcgg tgccgaagag cgatgaaagc gcgcgctcct cgggtccgat ctggaaacgg
     8881 ttcatgtaca gcacgaacac agccaggtag agcaaggccg gcgggctcgc caggaaggcg
     8941 acccacccaa agagcgtgag caacaggccg atgtacatgg gattgcgcgt gaagcgatag
     9001 acaccgttgg tgaccaacga cgatgtcgcg tctggcttga acggatggat ggtggttccc
     9061 gctcgccgga acagaaggat gccgcagata ctgatgctct ggccgatggc aacaagagcc
     9121 agcgccgcgc ccagtcgaaa tccgtacggc acgggccatg gctcgaaaag cagggacgtg
     9181 agccacatga gaagcgcaag gcccaatgcc agcaccggtg ggggaatttt gagttcgagc
     9241 gtgttcatgg cagttggcgt gcccgcaggg aaaatgtcaa agtgtgcggt gagatggcga
     9301 tgcggggggt ttgatcctcc ttcgatgcga gggctttgga ttcgcagcca cgacgcgcgg
     9361 cacgtgcctg gtaaagccgg ccggccagcg cccagagcct gtcctggtag ccgggatgct
     9421 cggtgccgca ccagcggtcc caccagacaa aatataggcc atagttcgca tgcccatggg
     9481 catgatgcaa ttcatggtgc agcgtggtgg tcagccagcg cccccaccag cctgcgagcc
     9541 aggcccttgg caccagttcg atgccggcat ggcccgccac gttgcgcacg atcatgtgcg
     9601 ccatccacag aaagaccacg aactggtgtg ccgggacaag cagcaggaaa aggggcagaa
     9661 aaatggcctg cacgaaggct tcagcaggcg cgaacgaata cgccgcccag ggtgtaggcg
     9721 caagcgattg gtggtgcgtg cgatgcaccc agcggaacag gcggcgatga tgcatgagcc
     9781 ggtgggtcca gtagaaccac gcgtcgtgca ggatgaccat gacaaagaaa ctggccaaca
     9841 gccagggcca gccgaattcc cggggaacaa gatagatttt catcaccccc gcgtggtacc
     9901 ccagcaaaac actggcaccc accagggaaa agacaagcac agtcagcatg gaatggccga
     9961 actcgcgcaa tggctgtcca ccggcgacct ggcggatacg aaccgttcgg cgttcccgcc
    10021 aggcggcggg tgcgcatgcc agggcgacaa ccagaccgcc cgcgacgacc agatagcgca
    10081 acaggtcagc cagccagatg cctggccagg ccgcgacgac cgcggcccaa taatcctgga
    10141 aacaggtcat gaagatctcc ttgacgttga agtgacgcca aatttagaaa aattgcggtc
    10201 aggcgtctgc ccgattccgg cgaatgcttg ccgttttatc gaaaaggcca gacattgaag
    10261 gttttggcga gatcggacgc cgcccttgtg acatgctcgg cactgccatg aacctgatcg
    10321 aacctttgct gcgcggtgca ggcatcgcca tggcgatcct gctgctcctg gtgactgctg
    10381 cgcgtggcgg ctggcgcctg cgcgccgact tgctgctgat gctgtcttgc gtgaccgctt
    10441 atctggtctg cgcctctccc gcccggctgt gcggcgccga gcccggcgcc ctgcccctgg
    10501 tgctggcagc cctggcgttt cctttcgcgt tttggcggct ggcctcggtg gtgctggagg
    10561 acgaccggca cgtccccagg gtggcttggg ctgctgccac ggccatgatt gttggcggcc
    10621 tgctggccgg ggtggactac ctgcctgtcc cgccgcactg gagggtggtg ctgggtggcc
    10681 tgagcaaggt gatggcaatt ggttttctgg gttcggccgg tgtcagggcc tggcgcagcc
    10741 acgagggtga tctgatcgat gcgcgccgtc gcctgcgctg gattgtgatg ggcgcgctgg
    10801 ggtgctacag cctggtcgtc gtgatggtgg agatctacct ccagcgctcg gccccgcccc
    10861 cctggctgga tctgctcaac atcacgctga tcgtcctggc gctgctggcc agcgcggtgt
    10921 tcctgctggg cgtgaggccg caggcccagg aagcgctttt cgaaccggcc gcgctacccg
    10981 gcaaggcaag cgagcccgcg tccttgcccg gcgccagggt ggacggtgat gcggcgctgg
    11041 ttgaccggct ggccgtcctg atgcgtgatc agcatcttta ccgcgacccg gaactttcgg
    11101 tcaggcgtct ggcctctgcc ctggaggtgc cggaatacgt gttgcggcgg ctga
//
LOCUS       DAIDFG010000074        11136 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_74_length_11136_cov_18.798574, whole genome shotgun sequence.
ACCESSION   DAIDFG010000074 DAIDFG010000000
VERSION     DAIDFG010000074.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 11136)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11136)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11136
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74_length_11136_cov_18.798574"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2049 a         3469 c         3584 g         2034 t
ORIGIN      
        1 ccggcttgcg gatgctgcgg atctgctccc agttgcgcgt gatgtagtcg cccttgtaga
       61 cgtcggcaaa gctgtagctc gccatcgcgc cgatgtcggc gccggctgca aaggcttttt
      121 cgctgccggt caggatcatg cagccgatgt tgtcgtccgc gtcgaacgcc ttgagcgcgg
      181 cgcccagctc gtccatcagc tgatcgttga gcgcattgag ttgcttgggc cggttcaggg
      241 tgacgatggc cactcggcct tcggtgcggg tctggatcaa ttcataagtc atggcgggtt
      301 cctttgaggc tgaatcatgc cacgatcttt ttgcatggac agcgcgccgt gaggtggaaa
      361 agcaagggaa aacattaacc gacctcacgg tcggttaatg ctagattcga cacaggagat
      421 gcttatgacc gatgtccctt ccgaacctgc cgtgctgttg tccacacggg gcgccgtggc
      481 gctcgtgacg ctgaaccgcc cgcaggccct gaacagcttt acccgccaga tgcaccagca
      541 gctctggacg gcgctggacg cagtggaggc cgatccagcg ctgcgtgccc tcgtgatcac
      601 cggcgccggc cgcggattct gcgccggtgc cgatctggcc gagttcgact ttgcgcccgg
      661 ccccaacctt gtcgaacgtg ccgatcccgg gcccatcatc gaccaggcct tcaacccgac
      721 ggtgcggcgc ctgcagaagc tgcgcgtgcc gacgattgcc gccgtgaacg gcgtggccgc
      781 tggtgcgggg gcctcgctgg ccatgacctg cgacctggcg attgccgcgc cgggcgccag
      841 cttcatccag gccttcagca agatcggcct ggtgcccgat gcaggcggct cctggtttct
      901 ggtcaagcgc ctgggcctgg cgcgggctct gggactggcc atgctgggcg acaagctcag
      961 cgcgaaggac gcgaaagagt ggggaatgat ctgggacgtg gccgccgagg gcgaagactg
     1021 cctggacaag gccatggcgc tggccgcacg cctggccgtc atgccgaccc gcgcccttgt
     1081 cgaaacgcgg cgcctgctca gcgaaggcgc cagccggggc ctggatgccc agctggatca
     1141 ggaacgggac acccagtcgg cgctgggccg cagccacgat tacatcgaag gcgtgatggc
     1201 cttcctcgag aagcgcccgc cagcgttcag gggcaactag atggatgccc agcaactggc
     1261 ccaggctgtg ggcgaatcca tgtttgccgc cgacagtgcc tcgcgcgact tcatgcagat
     1321 ggagctggtg tcctgcgcgc cgggccgcgc cgtcatgcgc atggccgtgc gtgagcccat
     1381 gctcaatggc cacaagatct gccacggcgg actgattttc acgctggccg actcgacttt
     1441 tgcctttgcc tgcaacagct acaacaaggt cgcggtggcg gcgggctgca gcatcgagtt
     1501 cctcaaaccc ggccagctcg gcgacgtgtt gacctgcgaa ggggtggagc agacgctgca
     1561 gggccggcac ggcatttacg acatgatggt cagcaaccag cgcggtgagg tggtggcgat
     1621 gttccgtggc aagagcgctc agattgcagg taccgtcatt ccggtgaccg gcgcggaggc
     1681 cgcatgaaaa gttttccact cgagccgatt gaaaaggcca gccttgacga gctgcgcgcc
     1741 ctgcagctca agcgcctgca gtccacgctg cagcatgcgt accggcattc cccggtgtac
     1801 aaggccaagt tcgatgctgc gggcgtgcat ccgggggact gcaagtcgct ggccgacctg
     1861 gcgaagtttc ccttcaccac caaacacgac ctgcgcgcca gctacccctt cggcatgttc
     1921 gcggtgccgc gcgagcagtg tgtgcgtatt cacgcgtcca gcggcaccac cggcaaaccg
     1981 accgtggtgg cttacacaag aaatgacgtg gacatgtggg ccggctgtgt cgcccgcagc
     2041 atacgcgcgg ccggtgcgcg ccccggcgac ctggtgcaca tcagctacgg ctacggcctg
     2101 ttcaccggcg ggctgggcgc gcactacggt gccgagaaac tgggcctgac cgtggtgccg
     2161 tttggcggcg ggcagaccga acggcaggtg cagctgatca ccgatttcag gcccgacatc
     2221 atcatggtca cacccagcta catgctggcg atcgccgacg agtttgaaaa gcagggactg
     2281 tccgccgccg acgccagcct gcgcaccggc atcttcggtg ccgaaccctg gaccaacgac
     2341 atgcgccttg cgattgaaaa acgcatgggc atcgatgccg tggacatcta tggcctgagc
     2401 gaggtcatcg gtccgggcgt ggccagcgag tgtgtggaaa ccaaggacgg cccgaccatc
     2461 tgggaggacc atttctatcc agagatcatc gatcccgtca gcggtgaact gctgcccgac
     2521 ggcgagatcg gcgagctggt gttcaccagc ctgagcaagg aagcgctgcc catcatccgc
     2581 taccgcacac gcgacctcac gcgcctgttg cccggcacag cgcgcagcat gcgccgcatg
     2641 gagaagatca cgggccgcag cgacgacatg atgattgtgc gcggcgtgaa cgtatttcca
     2701 acgcagattg aggagctgat tctcaagcgc catgagcttg cgccgcatta ccagtgcatt
     2761 ctgacccgcg agggcaacat ggacagcctg cgtgtggcgg tggagacccg ccccggcctg
     2821 gcgcccgaca gcccgtccgc gcaggcagcg gcccatgtgc tgcagcatga gatcaaggcc
     2881 tacattggga ccagtgtgga catcgagttg cgcgccgagg gcggtgtgga gcgcagcctg
     2941 ggcaaggcca aacgggtgct ggatcgccgg aactgagcgc cctctgtttc cgggtttttc
     3001 cggggtaagc accctcgacg ttgtactgtt ttggttttag actgacttaa caactagtta
     3061 caggaggttg ccatgcccga caagcaagtg atttatcaca agacacagaa ggggacggag
     3121 gcgattgcag accggcattc aggcctggcc ccccgactgc gctccctgtt gatcatggtt
     3181 gatggcaaaa gaacctacgg agaactgacc accatggccg cagcgctggg tgatccggag
     3241 cgtctttcag aacttgaaac cgggggcctg gtcgagccgg cagtggtcga ggaaaaaacc
     3301 atgccggcgg cgctggagcc ggcaggcgcc ccgcagctgc cgatcgcggc cacggccgca
     3361 gcgccgcagc gcgcggccaa cctggcgcag gcgcagcgtt tctcctcgca tttgctcgag
     3421 cacctgctgg gcccgatggc cgagccgctg tgtatcaaga tcgagggtgc gcgcgacctc
     3481 gccgattttg tcaccgtgat caagcgggcc cgcgagcttg ttcgcgagat caagggcagt
     3541 gccgaagccg agcgttttgt agcccagatc gaggcgcaga tgcctgctgg ctgaaccccg
     3601 ggttcaggcg gcggacacga gccacttttt gagcagcgtt gcgtccttgg ccagcaatcg
     3661 ccaggtggtt gtgtctggcg gcatcgtcaa ctcgagccga agcaggcgct tgtcgcgcgc
     3721 gaccagcgcc gtgatctttt tgctggggcc tgcgtaaaac ggcaactcgt cgaccttgcc
     3781 caggcgccag cccgggttgg acggcggggc cgatttccgg ctcttgccgg tcacaggggt
     3841 ttcaatgccc agccactcat cgccggcggc aaaaccggca cgttcggccg caccgccgcg
     3901 caatactgtc ttgatctgga tgccgcccgt ttcctgcacc cgcaggccca gcgcctgcgc
     3961 cagttgcgcc ggctcctcga gcacggcgac gccgtggcgc tgcagcaggt ctttcagcgg
     4021 cagctcgccc gtgccatgca cccagctggc gatttccgtc gcgtacgagc gaccgccgat
     4081 ctccagcagc gcggcggcga catccgcttc gctcatcgcg ccgccgcggc aacgcagcca
     4141 caggctgcgc atcacgtcgt ccagggtggc cggtatggcg ctcttgcttt cggcgcgcag
     4201 gctcaggtca agacacaagg cgaccaaggc gcccttggtg tagtagctga ccgtggcatt
     4261 cggcgtgttt tcatcctggc ggtaatactt gacccaggcg tcaaaactgg cctgggccac
     4321 cgactgcacc agccgccccg gggtctgcat gacctggttg atggtcctgt tcaggagttt
     4381 cagataagcc gcttcgtcga tcaggccggc gcggcacagg atcaggtcgt cgtaataact
     4441 ggtgaagcct tcaaaaaacc agagcagctg cgtgtaattt tcactgccgt agtcgtagct
     4501 gccgaactcg gcggggcgca ggcgcttgac gttccaggtg tggaaatatt catggctgat
     4561 cagcccgcgc agcgtggtgt acccctcggg cagcttgctg ctgcccaggc gcggcaagtc
     4621 ctggcgcttg cagatcagcg cggtggaatt gcggtgctcc agcccgccgt agccgtcgtc
     4681 cacggcgttg agcatgaaaa cgtaatgttt gtgcggcggc tttttgccgc cgtgccagaa
     4741 gcgtatttgt gcttcgcaga tcttccgggt atcggcaagc agccgctcac cgtcaaagct
     4801 ggccgtggcg ccggccacca caaagcggtg cggcacaccg caggcgacaa aagcgccgct
     4861 ccagaagctt cccatctcga ccgggcaatc caccagttcg tcgtagtcgg cggccagata
     4921 gctgccgaaa ccctgtttgt cgaccttcag cggactcagg ccggtggcca cggaccagcc
     4981 ggtgatggct ggcgcggcca tcacttcaag ccggtgcggt gccttgtcct ggccgtcgac
     5041 ccgcaggcat agggagctgc cgttgaagaa gccgcgaccg gcgtccagcg aggcggtgcg
     5101 taccgagctg tcgttggcat agacctcgta gaccagcacc agcggcttgt ccgcgttgca
     5161 gcggatctgc cagctgcatt tgtcgagctg cgcgatctgg cctgcaggca gcgcccgggc
     5221 gccttgccgc gcccgcaggc cctgcaggtt tttcgagaac tcgcgcacca ggtaactgcc
     5281 gggaatccac accggcagcg ccacccgctg cagcgcggcg ggctcggcaa tggtcagggt
     5341 gacccgaaac agatgggcat ggaggtcggc gatctcgacg cggtaatgga ctccgccgga
     5401 aggtgttccg gagggtttgg cagaaggttt gggcataggc tcaaagaata ccgcatcggg
     5461 cggagcgtgg cctggcgcgc tggcagctgg atgctggttt gttgctactg aaaacagagc
     5521 agttagcgac cgcctgcaaa gggcaggagc ccttttttat gcatgaacgc aggcggcacg
     5581 gaccgggttt actgggcctt ggcgtcggtc aggtatttct cgacctgctg ggcgctgatg
     5641 gcgccgggca cacgtgagcc gtcggcaaaa atcagcgtcg gcgtgccggt gatgcggtgt
     5701 tttttgccga attcgatatt gcgcgccagc gcgccggcgt cacaactggc attggccacc
     5761 ggctggtcac gcaccatcag gtcctgccag gccttggcct tgtccttggc acaccagatg
     5821 tttttggact tttccgtcga atccgcactc aggatgggat acaggaacat gtagacggtc
     5881 acgtcgctga ccttttgcat gtcgcgttca aaacgcttgc aatagccgca attcgggtct
     5941 tcaaacaccg ccaccttgcg cttgccgttg ccgcgcacaa tggtgaaggc gtccttcagc
     6001 ggaagcgcat caaacgacac ggcgttgagc ttgtcgacgc gttcttccgt caggttgcgc
     6061 ctgaccttgg tgtcgatcag gttgccctgg atcaggtagt tgccttcggc atcggtgtag
     6121 aaaatatcgc tgccgttgac gcgcacttca tacaggccgg ccatgggcgt tttgttgatc
     6181 tcgtcaatcc tgggcaggtt cggcaggcgc tcggccaggt tcttgcggat ggccgcttcc
     6241 tgcgcaaagg cgccaaggct cagggccagc agtgtgccgg tcatgatggt tttgaacagt
     6301 gtcattgttt tccagtctgt gtgattaacc ctcacgcctt tcacagcgtg tcttgccgta
     6361 tcttgcgccg cctcgccctg gggccgctca ccccggccgg tgcggcggcg aacccatggc
     6421 ctggcgcgtg acccattctt tcagcggtcc gctgcgtgca aagcccttca tgccccaatt
     6481 gcgcaaagcc tgccagcgcg cgtcgttttg cgcgaacaga ctgtgcagtc catccgtcac
     6541 cgcgcccatg gccgccacat cggctttgcg cgaacgttca taacgccgca acagcttttc
     6601 gtcgcccaga ccgcgccagt attcgcgccc gtgaatcacg gccgcgagtc cggccgcatc
     6661 ggccaggccc aggttcaggc cctggcccgc cagcggatgc acggcatggg cggcgtcccc
     6721 cgccagcgcc cagcccggac cgacccagcg ttcggcccgt gacagcgcca atggccagcc
     6781 cgacaggctg ctcgccagct gcagacaacc tgcttcggcg ccgcagacgg tttgcaccgc
     6841 cgcggaaaac tcttccgggg tcaacttctc cagcgcggct gcgcgctcca cagagacaga
     6901 ccagaccagc gccagagagt tcccgtcggt gccgccaatc ggcaaaagac ccagaatttc
     6961 gccgttgtgg aaccactgac gggccacacc ccggtgcgcc acgtcgctct gcagacgggc
     7021 ggccatggct ttttgcggat agggcttgac gatccattgc acgccgaact cgttgcggct
     7081 ggagcttttc tggccttcgc agatcaccgt gagcgcggcc ttgacaggcg cctcaacggt
     7141 ttctatctgc ggctggtaat gcacggcttc ggccaactgc tgctccaggg ctggtacatc
     7201 gacgatccag gccagggccg cgtcggcagt gccttgcggg gctgcaaaat cgagccggcc
     7261 cccgtcgtcg ccccagacct gcatttccag caccggcgtg gctgcagggg catcgggcca
     7321 ggccctcagc gaagtcagca gttgccgcga ggcgctgttc agggcgtagg cccgcacatc
     7381 ttgcgggaca gcggcttccg ccgggttgcc ggccggcgcg ggccgggcga ccaacgctac
     7441 gtgcaggcgc tcgcgtgcca gcagcagcgc cagcgtacgg ccgaccacac cgccaccgcg
     7501 gatacacaca tcaaaaggct tggacatggc tcattttaga aggcagggca aaatcgtccc
     7561 cggacagtgc atcttgaact gaatttttga agggtttgac acgtgcgtga atcaatgagc
     7621 gaaacatcgg accccgcgcc cggcgacgtc ggggcggtca ttaccgggct gtgggtttat
     7681 cccgtcaagt cctgcgccgg ggtgcaggtg caggaggcga tattgaccga aacggggctc
     7741 gaatttgacc gggcctggat ggtggtggat gaacatggcg ccttcctgac gcagcgtgag
     7801 ctgcctcgca tggcgctgat ccagccccag ctcaagaccc acgacatggt cctgcgcgca
     7861 ccgggcatgc tggcgctgca tattgcgctc gaccaggtgc aggagccggt gcgtgtgacg
     7921 atctgggacg acgaggtcaa ggcttacgac atggggccca tcgcggccca gtggttcagt
     7981 gatttcctcg gcgtgaaggc gcggctggta cgttttgatc ccgaggaaaa gcgcgcgtcc
     8041 agccggcaat ggaccggtga cgtggaggct ctcaaccagt tcagcgacgg ctacccgttg
     8101 ctggtgctca gcgaagcttc gttggcaggg ttgaatgaaa aactgcaggc cgcgggcgct
     8161 acggctgtcg gcatggcgcg tttcaggccc aacatcgtgc tgggcgacct gtcaggcgac
     8221 aacgggctcg cgccgcatga tgaagaccgc cttgagctgc tgcagattgc cacggaacag
     8281 ggcgtggtgc aactcaagct ggtcaagccc tgtccgcgct gcccgatacc caatatcgac
     8341 ccggtcacag ccttgagcag ccccgaagtc ggcgacgccc tgcagaccta ccggcaggac
     8401 ccgcgcctca agggagcggt gagttttggc atgaatgcca tcgtgctgca gggcaacgac
     8461 cacctgctca gggtggggca gagcgtcacg gccaactaca acttcgggta attccggacg
     8521 ggcttgctat cgaggtcgcg gttgctgtca tccctggaat ccccatccag cggcatgggc
     8581 cgggtcagct cgggttccag gaaacggatg ggccggcgtg tgcgcgccgc catgctgtcg
     8641 agcaggtggg ccagtgcgtg cgagagttcc ggcccgtctg ccagcttttc gctgagcagc
     8701 acgccggcga cgtgaaactg caggacaacg tcgggctgga gtcctttgag cggcatcagt
     8761 cccgcggcga tcagccgcgc ggccatttca ttgaccggct cacgcgatga cacgccttcc
     8821 aggatcattc cgaatctggc ttcatccacc cgtccgacgg tgtcgacatc ccgcaggatg
     8881 cgacgcaact tgatcacgct gcgcagcagg ctttgttcgg ccacggctgt gccgtaggtg
     8941 cgcttgacat aatcgtagtt ggccagctcc acataaacca cggcagccgg ttccccgtgg
     9001 cgcatggcgc gagagaccac ccggtgaagc cggtcgttga acagatgcgg ggtcagcagg
     9061 ccggtcagcg catcctgggt tgccatggcc tgctccctgg cttccatgct gtggcgctcg
     9121 cgtgagcgca ggttcaacgc gaccagcagc aaaggcacct ggagggtcag gccggccagg
     9181 agcccgtatt gcgatagcga tgagaccgga agcaggccca tggtgctggc cagcatcagc
     9241 agggcagcca gcgccaatgg catgaaggcc gcaagtaccc agaaccccac catgtccctg
     9301 cctttccaga cgatgatggc tctgcgcatg cccagcacca caaccgcaat cacataggtg
     9361 ccagcaatga tgacaccggt gccgcgttcc accatggcat aagtgattgc cagcggcagg
     9421 ctggccgcgc caagccagta cgcgagcggc tcaaaccact tttggccggc gccggggcca
     9481 cacaagtgat gcacgaccat gggcgccgtg ccacccacca gaatgctcag gcagcccggt
     9541 gccaggtcgg cccagacacc ggaatcgctc cacaacaaat ggctggccac gccagtccag
     9601 gcggccgcca ccagggtcat gaccgacaca tacagggcat accagccgta ggcgcggtcg
     9661 cggtagacca ggctctgggt ggcaccggca acaatcagca aaagcagggc gcccagcacc
     9721 acacctgtgg acagagcctc gacctgctgg cgatgactgc ggactttgcc ggagatggcc
     9781 ttgatcggga tgttgacacg tgtcgcatgc tggatgcgaa catacacgtc gtggacgctg
     9841 ccgctcctca atgccagctg gaattgcgga tagcggccag gctccggcca gagggaaacg
     9901 gcgacggtgt cgccagccgt ctggccgatc cagtcgcctg tggggctccg ctggaagacg
     9961 gtgaccaggt ccagcagggg caggggaaac tgcagcaccc aatcttgccg cgagtcggct
    10021 gatttcatga agcgatagtg ttgccacagc gcggcctggg agcccagcgc gtaaacagta
    10081 tgggcttgcg cgggactcat gggcggacgg gttttgctgg acgccagctg agtgatggtg
    10141 gcctgggcgc cgctgtcgat ccaggtcagg ccactggtct gcaggtctgt gacgggacgc
    10201 tgatcgtcga gaatgacgat gggctgggca aaactggcgg cgccgagcaa accggacaga
    10261 accgccagca acagggctgc cagaaagccg atcgcgtatc gcatacactt cacagcctgt
    10321 ttttgaaggt ttttcatgag cctgaagtgc ggtattgtag gctggcccag ctcggtaaat
    10381 cgaccctgtt caacgccttg acccaggcgg gcattgcagc gtaaaactac cccggcaggt
    10441 tgtacgatct gaacaggcca agggcctcaa gcacttcctg gaactcgcaa agctcaggcg
    10501 acaaaaccag gacaaaggca cccctcgaaa acgcatcaac tggatcagac catcatggaa
    10561 gagaggctgt ttttttggga cttgccggcc ccgggtggca tgccgaaaca actggtcctc
    10621 aaggggcaaa tgcacgccgc cggacttcgc ttatccactg ctacttcatc aattcagtcg
    10681 aacaaggatt tatatggatt ttctgggtct ttttgaaaag tactggccat ttatggctct
    10741 ggcgctttgg ttttcgtaca agtggtggaa ctcaaaaaga gttatcgcca tgctgccgga
    10801 actcaggaag agcggcgcga cctttgttga tgtcaggtca gccggggagt tcgcaaatgg
    10861 gaacgcccct ggaacaatca acattcccct gcaggagttg ggtagcaggc tcggtgagat
    10921 acccaagtcc accccggttg ttctttgctg tgccagtggg acacgaagtg gaatggcaaa
    10981 actgatgctg aggaagaagg gctaccggaa cgttcacaac gtcgggacct ggagcaagtt
    11041 gttgggctga tctgcttttt cgaagccgca aaggcccccg atctccgggg atgcgtccag
    11101 cgggtgcgag cgtgctaagt agccaggatt tgctgg
//
LOCUS       DAIDFG010000075        11037 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_75_length_11037_cov_19.311328, whole genome shotgun sequence.
ACCESSION   DAIDFG010000075 DAIDFG010000000
VERSION     DAIDFG010000075.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 11037)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11037)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11037
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75_length_11037_cov_19.311328"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1819 a         3618 c         3743 g         1857 t
ORIGIN      
        1 ccgcaggcgc agcggccccc tcggggggca gcgaaccaca cgaagtgggg agcgtggggg
       61 cctatctaac ggaccggcag gaactgcaga aaggaagcgc ccgccaggct ctgcacatag
      121 gtgatcacgg cgcggcggta tttctcggtc aggacttcgg agagcaggtc gagccggcct
      181 atcaccttgc cgaactcgcg ctcgaaactg aggttgcgct cgcgctcgga gatctcgtct
      241 gccagcagca ggggctggcc ggccgggttg cgccgcgagg ccagcagcca gacggcggac
      301 tcgatgttgc gcgcggcgtt gtagatgtat tgcgcatcca ggctgtcgac caggtagaag
      361 tcggtcttgc cgttgtgcgc cacgatgagc atgtcggcgc tggcgtacac aaaagcggcc
      421 acccggtcgc cccggaagtc ggggctcagg gccagcgaca gggcggcgat gtcgcgtttg
      481 ccctgcagct ctggccaggg cgtgcggctg tcgatggcgc tgcgcacgcg ctggaccgcc
      541 gcctcgcggc tggtgctgcc ggtcttgcgc cattcggcgg ggttgcgccg gtaaagcttg
      601 tcgagcaggc ggtacaggct gcccaggttg tcgcgcatgc ccagggtggc catgcggttc
      661 acgtccgatt gcgccagctc gcccgaggtc agcggcgcca ccggcgtctc gccgcggtgg
      721 gtcggggccg tctggaggca gccgcccagc agcagcaagg ccggcagaca gaacagggac
      781 aacaaggaca acagcagagg ccggcgcggg gagggcatgc ggcgattatc ccggagccgc
      841 cgcagggcgc aagcgccgcg gacccgtcag ccccccggca gcaggccgcg cgacttcagc
      901 accctgtggt caaccatgag gcagtggtcc tgcaccacca gcaggcccgc cgcttcggcc
      961 tgggcggtgg ccgccgggtg gcggatgccg agctgcatcc agacggcctt ggcgccaatc
     1021 gcgatggcct cgtcgacgat gggcggaatg tcctcgctgt tgcgaaagca gttcaccaag
     1081 tcgatgggtt cgtgctgcgc ggcttccgtc aggctggcat aggctttttc cccgagcact
     1141 tcggtggcat tggggttgac cgggatgatg cggtaaccct ggccctgcat gtaacgcgcc
     1201 acgtcgaaac tggcgcgatg cggtttgggc gacaggccca ccaccgcaat cgtgcggcag
     1261 tgggtgagga tgtagggcag gcgttcggcc ggcgggaggg cgttcacgga gggactcatg
     1321 ccttgtactt gatcgagcag ccataggcgc ggctgcttgc cacgctgagg gccttgccgg
     1381 ccagcgcttc gttcaggccc tggcgcacat aattggttgc ggtccgtatg tcgtccacac
     1441 gggcggaggg cacgctgtcg atggccccgg catacaccag ctggccctgc gggctgatga
     1501 tgtacatgtg cggcgtggtc cgggcgctgt agagctggcc gatgctgccg gactcgtcca
     1561 tcagggtggc gctggccgcc gcctgcttgt tgcgcatcca gccggcaagc tgggcgggcg
     1621 ccaggtagtc gccgctgccg gtttcggtcg agttgatggc cagccagacc acccctttgc
     1681 cgaccgcctc tttctgcagg ccctgcatgt tgccgctgta gtgtttttgc acaaacgggc
     1741 agccggggtt ggtccattcc agcaccacga gcttgccctt gtagtcgctg agtctatggg
     1801 gttttccggc ggtatccacc gcagaaaagt ccggcgcggc ctggccaacg gccgcatggg
     1861 cggtctgcgt gagggtcgca gcccacgggg cggtgagcag agcgctggaa aagatccggc
     1921 gattgacgcg gtgattcatc atggcttgat ctccttcaga aaaatgaagt gacagatgaa
     1981 gtgacaggca gtactacagc ttggccagtt cggcccgcac atcggcaacc gacagaattt
     2041 ccgacagcac cacaggcgcc cggccgggct tgtagatcac atacaccggc acgccgttgc
     2101 ggcccagttg cgccagcgcg gcggtcacgg ccgggtcgcg gcgcgtccag tcggcgcgca
     2161 gcagggcgac gtttttggca tccatgtcgg ccagcacatc ggcatcggcc agcgtggttt
     2221 tcttgttgta ctggcaggtc acgcaccagg cggcggtgaa atcgacaaac acgttctggc
     2281 cttgggctgc gagctggtcc acgcggcccg gttcccaggc ctgccagcgg cccgaggctg
     2341 ttgcggcgac gctggatgcc tccagcggtt tgatgacatt ttggcccaca gtccacgccc
     2401 cgaaagcgcc aagcgctatc gaaagcgtgg cggccacagt ccgggtccgg ccccgcagtg
     2461 tcagcgacca caacaccagc gccatcagca ccagcagcgc cagcagggcg ccggcaccgt
     2521 cgatgccgct ttgctggccc agtacccaga ccagccaggc gacggtggca aacatcggaa
     2581 acgccatcag ctgcttgaag gtcaccatcc acgcgcccgg tcgcggcagg gcgcgcgcca
     2641 ccgccggcac cgcactggcc gccaggtagg gcagggccat gcccaggccg atggcaccaa
     2701 acaccgccag cgcctggacg gccggcaggc ccacggcgta accgagcgac gcccccatga
     2761 aaggcgcggt gcagggggag gcaatcgcgg tggccagcac gccggtcaga aaggcattga
     2821 gcgtcgggtt tttggcctgc aggccggcga ccttgccggg cacaaagctg ccaaattcaa
     2881 acaggcccgc gaggttcagg ccgatcaacg tgaacaacac ggccagtccg gccaccacgg
     2941 ccgggctttg cagctgaaag ccccagccga gctgttcgcc ggtggcgcgc aggcccagca
     3001 gcagggcgcc cagcgccagg aaggacaaca ccacgccggc cgtgtaggcc agcccgccgg
     3061 cgaggcgtgc cgcgcggttt ttttccttgg cgaaactcat caccttgatg gccagcaccg
     3121 gaaacacgca gggcatgagg ttgagaatca gcccgcccag cagcgcaccg agcagggcgg
     3181 cgaccagcgt gagcggcggc gcgctgttgc cggtgggcac tgcgcccagc gccgcattgg
     3241 ccttgagcgc tgcttccagc gcgggcgaca ccgtggccgc ggcggccacg gccggccagt
     3301 cgccctggac cggcagctcg atgcggtagg cggtcttgtc cagcgccacc accaccggca
     3361 tcacgcgcgg gctttcgctg cgctggctcg agagcggcag ctgcgcggtc cacagcgcgc
     3421 cctgccattt ctgctgccag gcgccggcgg gctcgatcac gctgccggtc tcagggaaaa
     3481 agtccagggt cttgccctgc agacgcacgg gcaggcctgc gagcgtcacc tggagggtgt
     3541 tggcgctgac ctgcacctgg ctgccggtgg cggccagcgg ctgggggctg gcggcaaagg
     3601 cggcggcaaa caggccgctg ctgctgccgg tcgaactccg gaccgggatc tgcagggaaa
     3661 aatcaccttc ctgcggaata cattcctttt tgcagaccag ccagctggcc ttgagccgga
     3721 tgtccaggtt ggaggcattg aagccgggcg ccacggtcag cggcaccggc agcagcaccg
     3781 tgctttcgta gccgtagttg gcgagggtgc caatcggtat ctttttcggc gtgggccagg
     3841 cgatgtcgcc agccgtgacg ccggccggca gcgtccattc gagctgggtc ggcaggccgg
     3901 aatcgccgga gtttttccaa taggtgtgcc actcggggat gtgcgccagc tgcaggccca
     3961 cccagaccgg ttttcccggc tccacgccat cgggcgccca ggccagcagt tcggcacgca
     4021 cctggtcggt gctgaccacg gcattcgccg cggccttgtt ggtgaagttg atttgggcgg
     4081 cggcgcccgt cgttactgcg caagcagcta tcaaaaacag agcggacgcg aagcggtgaa
     4141 aaatcatggt catggtgtga gcggcacgct ggcctttagt tcctgggacg gtgcccgcag
     4201 gccttattgt ccggcagagc ccgtgggcag gcctgaacag ggttcaatcc ggcccgggca
     4261 gcagcgaggg ctgcgcaaac accgccggca gctttttcgg cgcacggatg cgcagctgct
     4321 tgttgacgtt ttcccggccg aaaaccactt cgatgtcgct gaccgccacg ccgaacagcg
     4381 gcgccaggaa acgcagcatg tggtcggtgg ccttgccggc ctgcggcgcc gcggtcacgc
     4441 tgaccttgag ctgggtgccc ttgggtttgc cgatcgcgtc acggctggcc gacggtttgc
     4501 ccaggatgtt gaccaccagc acctcgccgt cccaggcaaa aaaggagtcg ccggtgatgt
     4561 ttctcggtcg catcggagaa agatcaggcg gcgaagccca gcaccacgcg gcgcgtggtg
     4621 gcgctttttt tctcgatttt ggtgaccacc accgcgccga tctcggacgt gttggccaca
     4681 tgggtgccgc cgcagggctg gaagtcgatc atggcgtctg ccccggcgcc gcctatgcgg
     4741 atggtgcgca cctggccgct gccgcgcggc ggctgcacac tcatgctttt gaccagggcc
     4801 gggttcgcgt cgagttccgc gtcgctgatg ctgcccacgg ccaccgggtg cgcggccgcc
     4861 accaggcggg caatgccggc gctcaggacg tccttgtcca gcgggtcggt catgttgaag
     4921 tccagccgcg catagtcggg cgtgatcgag cagccgttga cgagctgcgg caccaggtgg
     4981 cacagcaggt gggtggtggt gtgaaaacgc atgagccggt gccggcgcgc ccagtcgacg
     5041 cgggcggtca ccacatcacc gggtttcacg cccatgcgca aaaggtggtc gagcaccgac
     5101 gtggccgggt cgggcaaggc cggcaggtgg cagatgtccg cggtgaactg gccttctgcg
     5161 tttttgccct tgcgcgtgtc ggcgatgggg atttcacgcc catcggccag caccagcaca
     5221 ccggtgtcgc cggcctggcc gccgcccagc gggtaaaaca ccgtgcggtc cagcaccagg
     5281 ccttgttcgg tcaccgcggt cacggttgcg ctgcattcag ccagataagc gtcatcacga
     5341 aacattgttt gtgtcatggt ttgataatac cggcatgaac tccctcgcgc cttcgcccct
     5401 tccccctgag ccccggggcg gggcgcgtat tgtcctggcc acgctcaacg ccaaatacat
     5461 ccacgcctcg ctgggcctgc gttacctgct ggcgaacatg gacaagcatg gcggcgcggg
     5521 cctgcgcgcg cagacacagc tgcgcgagtt tgtcatcggc cagcccgaga cccgcatggt
     5581 ggaagagctg ctggcgctgc agcccgaggt gatcggcctg ggcgtgtaca tctggaacgt
     5641 ggtggaaacc acgcagctgg tgcgcctgct gaaaaccctg aggcccgaca tcaagattgt
     5701 gctgggcgga cccgaggtca gccatgaggt gggggagcag gagatctgcc gcctggccga
     5761 ccatgtggtg accggctggg gcgacgtgag ttttcctaaa ctctgccgag cgctgcttaa
     5821 attgccaggt gggggcccga agccgctgat gaaggtcatc gccggcgagc aaccgccgct
     5881 cgatgaactg gccctgccct acaaggagta cacggccgag gacctggcgc agcgcctgct
     5941 gtatgtcgag gcctcgcgcg gctgcccgtt caagtgcgag ttctgcctga gctcgctcga
     6001 caaaaccgct tgggcctttg acctggacag cttcatggcg gaaatggacg cgctgtaccg
     6061 ccgtggcgcg cgcaacttca agtttgtgga ccgcaccttc aacctgaaaa tcgacaactc
     6121 ggtgcgcatc ctgcagtttt tcctggaccg catcgccgaa gcacccgatg tgttcgtgca
     6181 tttcgaagtg gtgcccgaca gcctgccgga tcgcctgaaa gaactgattg cgcagttccc
     6241 cgacggcgcg ctgcagttcg aggtcggtat ccagagtttc aaccccgagg tgcagcaacg
     6301 catctcgcgc aagcaggaca atgacaagac cgccgacaac ctgcgctggc tggtcacgca
     6361 aagccgtgcc cacgtgcatg ccgacctgat cttcggcctg cccggtgaga cgctgcagag
     6421 ttttgccgag ggcttcgacc gcctgcacgc tctcgcgccg cacgagatcc agttcggcgt
     6481 gctcaagcgc ctgcgcggca ccccgatcac gcggcacacg ctgaactttg cgatgatcta
     6541 tgacccgcac acgccctaca ccatcttgca aaactcgacg gtggattttg cggccatgca
     6601 gcgcatcaag cgttttgcgc gctactggga gatggtggcc aattcggggc ggtttgcctt
     6661 gagcctcgat ctgctgctgg cgcccggctc ggccttcggg catttcctgg ccttcagcga
     6721 ctggctctgg cagaccacgg gcaagaccca tgagtttgcg ctggaaaaac tggtggacct
     6781 gttgtatggg catttgacgc aagtgcgttt gctcgacccg caggctgtgt gcgctgcctt
     6841 gctggcggat taccaggcca gcggtgcgcg cgggcggccg cagtgtctgg cggcgttgct
     6901 gggcggtccg cagaaagcgc tttcggcagc ggcggagcta cacggggctc ggcggcaggg
     6961 tcggcatctg agccagcagc agaatttgcg ggtggagatt cagaaggcag cggcggcggc
     7021 ttagttttga accttcgaac ggcagaacac ccccagcgca gaatggccca gaatacgccc
     7081 atgaaccccg cgcccccctc cccggcatcc tttccccatt tctgggccga cctcaaaagc
     7141 cccgacttcg cccgcctcga tgtcgccgcg accatcgccg tgttgccggt cgccgcgatc
     7201 gagcagcatg gtccgcacct gccgctcagt gtggacaccg acctggtcaa tggcgtgatt
     7261 gcacactgcc tgccgcatct ggaacaaggt ggcccggtgc tcttcctgcc gacccagacc
     7321 gtgggccgca gcatcgaaca catcgccttt cccggcaccc tgaccctggg cgcggccacg
     7381 ctgatccaga gctggatcga cctcggtgaa tcggtcgcgc gcaccggcat caaaaagctg
     7441 gtatttctca acgcgcacgg cggcaatgtc tccacgctgg acatcgtggg ccgcgagctg
     7501 cgcgcgcgtt tcggcatgac ggtgttcacc gtcaacacct tcagcctgcc gctgccggct
     7561 gccgtgaagc agctgttcgg ccctgacgag gaacgcttcg gcgtgcatgg cggcgacgtg
     7621 gaaacctcca tgatgctggc cttgcatccg cacaaggtgg acctggcgct ggcgcagaac
     7681 tttcactcca gcagccagga ccgggcgcag caacacgcca tccttggcaa cggtagcagc
     7741 accaaactgg cctgggccgc gcaggacctg aacccgctgg gcgcggccgg caatgcggcg
     7801 gcggccacgg ccgaaaaagg cctggcggtg ttgcaggcca gcgggcaggc gctggcgcag
     7861 ctgctgcacg aggtccgcca actcccgccg ctggcctgaa gcgacgcgcg gccgtcctga
     7921 caaaaactgg ggacatcagc tgacagagca cagtagcctg aagggtcaac aatcagcagg
     7981 acgcgcgacc cacaagcgcc gcccatcacc gaacaaggat cgaccatgcc cgccctgcct
     8041 gttgcccgcc ccgcctcccg tgtttcccgt ttcgtgctgt tgccgctgtg cgcagcgctg
     8101 gcgctgggcc tgagcgcctg tggcgacacg accaagctgc ctgccggcgc cgatgtgggc
     8161 atcacaccca cactgcccca gccggtcaag tcgctgatcc ccaccgtgaa catcgctccg
     8221 gcccagggct ggccccaagg cgtgatgcca cagcccatgg ccggaaccac ggtgacagcc
     8281 ctggccaccg gcctggacca tccgcgctgg gtctacaccc tgcccaacgg tgacgtgctg
     8341 gtggccgaga gcaacaagcc gccccagccc gacgccggtt tcagcatcaa gggctggatc
     8401 atgggggtgg tcatgaagcg ggccggcgcc ggcgtgccga gtgccaaccg catcacgctg
     8461 ctgcgcgatg tggacggcga cggcaaggca gaaacccgcg cggtgttcat gcagggcctg
     8521 aactcgccat ttggcatggc gctggtcggc aaggagctgt tcattgccaa tgcggacgcg
     8581 ctggtcaagg tgccgtatga agagggccag accgcgatca cggccacgcc ggtcaaggtg
     8641 gtggacctgc cggcgggcct gaaccaccac tggaccaaaa acgtcatcgc cagccgcgac
     8701 ggcagcaagc tgtatgtcac tgtcgggtcc aacagcaatg tcggtgaaaa cggcatggac
     8761 atcgaggtgg accgggcggc catccacgag gtcgacccca caaccaacac gcgccggctg
     8821 tttgcttccg gcctgcgcaa tcccaacggg atgggctggg cgcccgacgg cacgctctgg
     8881 accgtggtca acgagcgcga cgagatcggc agtgacctgg tgcccgacta cctgacctcg
     8941 gtgaaggacg gcgcgtttta cggctggccc tacagctatt acgggcagac ggttgatgtg
     9001 cgcgtgacgc cgcagcggcc ggacctggtg gccagggcga ttgcacccga ttacgcgctg
     9061 ggctcccacg tcgcgccgct gggcatggtg tttgccgaag gcaacacgct gcctacgtcg
     9121 ctggccagcg gcgtgtttgt cggccagcac ggctcgtgga accgcaagcc gctgagcggc
     9181 tacaaggtgg tgtttgtgcc cttcatcggc agcaaaccct cggacaaacc cattgatgta
     9241 ttgaccggtt ttgtcagccc cgacggaaag gcctatggcc ggcccgtggg tgtcgccctc
     9301 gacaaacgcg gcgcgctgct ggtggccgac gacgtcggca atgtgatctg gcgggtggct
     9361 ggaacgccgg tgcgctagtc caggggcgat cccggtctgc gccagtgatg ttgcataaaa
     9421 tcgaccttcg gcccacgctg gaaaagcgcg atcagctctt tacttgatag cagattgccg
     9481 gcgccggttt atcgcaacgt ggccagatag gccgccatgt cgcgcgcgtc aagctcgctc
     9541 acgcccatgt cgggcatggc cgtctgcggc ttgaccgctt tcgggtcacg gacccacaac
     9601 accaggttgt cgggcgtgtt gaccagcttg ccggcgatca ggctgcggct ggccagaccc
     9661 tgcagcggcg gcccgacatt cggcaaggcg ctggtgatgc cggggatggt gtggcaagcc
     9721 tggcaggcgt attggcccag cgccagcttg ccgcgttcca catcgccggc gcgcaggggt
     9781 gcggcggcag gcgagcgctg cggcgcggca ccagcggcct gggtcatctg cgcataggcc
     9841 tgcgggctca gggtgggcaa ggtggccaca aaggccacca cgtcccatag cgcttcatcg
     9901 tccagccgga actgccaggc cggcattccg ctcatcttga tgccgtggcg cgtgatccag
     9961 tagatctcgc gcgggcgcca gcgctgcagc gcgtccacca gcggacccgg caccggctgc
    10021 atgcttttgc cgatgtccga ctgcgccaca cctggcgcac cgtggcactg cacacagtgg
    10081 tcgcggtaca gcgcggcgcc gcgctgccgc cgcggcgcat cgtccagcgc cggcgtctcg
    10141 atgtggcggg cgcgccagcg taccgagttg cgcatggtgg tttcaagcag cgaatgcaca
    10201 ggttgcaggt gttgcgctgt agcccccacg ttgtacacgc cgctgtagac cacgaagccc
    10261 gcgccggcca gcgcagcgcc cagcagggcg gtgacggtgg tgagcaccag cgtggtgttt
    10321 ttcatcgcta ctattttgcg ttgaccgcgt atgggagatg gcaagtgtcg gcgctgtcct
    10381 gcaaggattt gcgcgcgcgt ggagaagaat cgggcatgcc gatttttgac ccgctgtccc
    10441 gtgccacctc gctgacgctg ttggcgctgg ggcttgcggg cctggccgcc tgcggcgccg
    10501 atgccgccga tcccgcaccc ccgctcaagc cggtggcggc cggtgacgcc gcccggggtc
    10561 tgcgcctgct cacgcagtac cagtgcggca gttgccatgc gattcccggc gtgccggcgg
    10621 cgcgcggcat ccacggaccg accctgcagg ccttcggcaa acgcagctac atcgccggcc
    10681 gcgtgccgaa tttccccgaa acactggcgc agtggatggt tgcgccggcg tcgctggtgc
    10741 cggacacgcc catgcccagc atgggtgtct cgcccgagga tgcgcgccac atggcggctt
    10801 acctggggca gctcgagtga gcgcgccctt gctgccgctt gcgccgcaac tccagtcggt
    10861 gctggcgccg gccggcgagg ccgccgcccg catcaccagc ctgagctggc tgctgatcat
    10921 cggtgcgacg ctgatttttg tttttgtcat ggcgctgctg gccgggtctc tccgcagacg
    10981 cgatggcgcg cgaccggtgt cctcccggct gtggatcctg ggcggaggcg tggtgtt
//
LOCUS       DAIDFG010000076        10982 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_76_length_10982_cov_17.773497, whole genome shotgun sequence.
ACCESSION   DAIDFG010000076 DAIDFG010000000
VERSION     DAIDFG010000076.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 10982)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10982)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10982
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76_length_10982_cov_17.773497"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1866 a         3564 c         3717 g         1835 t
ORIGIN      
        1 ggatgtggtc gatggcgagc gcatccgcac cgatgggcgg gcacagctca acctgtcgga
       61 aagcctggcg ctggtgcccg gcctgctggc gcgtgaccgg cagaaccagg cgcaggacct
      121 gcagctgtcg gtgcgcggct ttggcgcacg ttcgaccttt ggcgtgcgcg gcgtgcgcct
      181 gtatgtggac ggcattcccg cgaccatgcc cgacggccag ggccagctct cgcacatcga
      241 tctggcctcg gtcggacgcc tggaagtatt gcgcggcccg ttttcggcgc tgtacggtaa
      301 ctcgtcgggc ggtgttttgc aggtcttcac cgaagaaggc cagggccgcc cggtggtgag
      361 cagcagcgcg gcgacggggt cccatggcct tcggcgtttc gacgtcaagg ccggcggtgc
      421 cgccggggtc ctggggtacg ttctgagcgc cagccgcctg agcaccgggg gttatcgtgc
      481 gcacagcgcc gccgaacgcg agggggccaa cctgcggctg gactgggcgc tggacagcga
      541 tcgcaagctg accctgctgg ccaacacggt gtcggtgcag gcgcaggacc cgctgggcct
      601 gagccgcgcg cagttcgatg tcgcgccaca cagtgccgat gcctcggcga ccaccttcaa
      661 cacccgcaag accgtgcgcc agagccagct cggcgcggtc tacgagcagc gggtcgatgc
      721 tgaccaccag ctgcggctga tggtgtacgg cggcgagcgc gccaccacgc agttccaggc
      781 catccccacg ggcgtgcagg ccaatccgtt gcaccccggc ggcgtgatcg gcctggcgcg
      841 cgactacggc gggctggacc tgcgctggac cagccgcacc ctgttggcgc agcgcccgct
      901 ggagatcgtc ggcggcctgg cgtatgacgc gatgaaggaa acgcggcgcg gctggcagaa
      961 cttccaggga acaacaccag gcgtgcaggg cgccgtgcgg cgcgaggaag acaaccgcgt
     1021 caccagcctg gacccctacc tgcaagccac ctggaagccc gctgcgcact ggaccgtgaa
     1081 tgccggggtg cgccacagcc tggtgcgttt cagttccgcc gaccgctaca tcgtcgggac
     1141 caatggcgac gacagcggca gcgtgcgcta tagcgccacg ctgccggcgg ccggtatcac
     1201 gtatgcgccg tcggcgcagt ggcgtgtgta cgtggccgcc ggccgcggtt tcgaaacgcc
     1261 gaccttcaac gagctggctt accggccggc cggcaccggg ctgaactttg cgctgcgccc
     1321 gtcacgcagc gacaacctcg aagccggcgc caagtggcgc agcgccgccg gagcaacgcc
     1381 acgcatcgag gccacggcgg ctgtctttca tacggcgacg caggacgaga tcgttacgca
     1441 gtccaatgtc ggcggccgca gcaccttccg caacgccggt gccacgcgcc ggcgcgggct
     1501 ggagctggca ggcagcatcg tgcccgcacg cgactggctg gcgcagctgt cctacacctt
     1561 gctggacgcc agctaccgcg acgcctttgc ctgcccggtc aacgccagcc tctgtccagg
     1621 aggcgtggtg gcggcaggca accgtctccc cggcaccgct cgccacgcac tggccggcga
     1681 gtttggctgg cggccgtcca ccggctggcg tgccggtatt gacgcgcgtt acctcggcag
     1741 cgtggccgtc aacgatgtca acagcgatgc agcggcgtct tttgtcacgc tgagcgccca
     1801 tgtcggctat gtggccgaga tccagcgctg gaccctgtcg gctgcggccc gcatcgacaa
     1861 cctgaccgac cgccgctacg cgggctccgt catcgtcaat gaaggcaatg gccgctattt
     1921 cgaaccggcc gccggccgca atcacgtgtt gagcgtgtcg gcgaattacc ggttttaggc
     1981 ggcgcggcac aatagccgct cccgcacaac gcaccgactt ttccccatgc acaccaccgc
     2041 cctcgtcctg ttctccggtg ggcaggactc caccacctgc ctggcacagg cgctgtcgaa
     2101 atacgagcgt gtggaaaccg tgggtttcga ctacggccag cgccatagcg tggagctgca
     2161 ggcgcgcctg gtggtgctgc gcgagatcag ggcgcagttt ccgcagtggg cgtccaggct
     2221 tggcgaggat cattttctcg accttgccgt gctggggcag gtcagcgaga cctcactgac
     2281 acgcgacacg gctttcaaga tggagagttc gggcctgccc aacacctttg tgccggggcg
     2341 caacctgctg ttcctgacac tggcggcggc gctggcctat cggcgcgacc tgcaggtact
     2401 ggtcaccggg gtctgcgaga ccgatttttc aggctacccc gactgccgcg acgacaccat
     2461 gaaagccatg caactcgcct tgtcgctggg catggacaaa cgtttcctga tcgagacgcc
     2521 gctgatgtgg atcgacaagg cggccacctg ggcgctggcc gaggcgctgg gcggccaggc
     2581 gctggtgcaa ctcatcatcg aacacaccca cacctgctac ctcggcgacc gggagcaccg
     2641 ccatgcctgg ggttatggct gcggccagtg cccggcctgc gagctgcgcc tgcggggtta
     2701 tgcggcctat gcggccgcgc tgtcgaagcc gtagtcgcgt tcaacgcggg cgatgcgcgt
     2761 cttgtaccgg cggtaccagt cactgcgccc cgtcttttgc gccaccaaat gctcggcgtt
     2821 ggtcttccag gcccgtatgg cctccaggct ctcccagtaa ctgacggtga tgccgacacc
     2881 ttcccgcgcc gattccacgc ccagaaaacc cggctgctgc gcggccagtt cgaccatgtg
     2941 ttcggccatg gcgccgtagc ctgcgtccac ttcggtgcgc agggaggtga agatcacggc
     3001 gtagtagggg gctggcggtg tggcggcgat catgcggatg tctccttgag ctggtacagc
     3061 gctgtcgcgc cttcgggttg tttctccagt tcccagcgct tgccgtgaaa cgcggcgacc
     3121 gtggggcggt gcgctatgga aacaatagcg cctcccgctt gcctggtttg ggccagcagc
     3181 cgttcataca gtgttttttc ggcgtcctcg tcgagcgcgc tggtggcttc gtcggcaaac
     3241 agccaggccg gctttttcag cagcacgcgg gcaatcgcca ggcgctgctg ctccccgcca
     3301 gagagtttct ggctccaggc gtcttcatcg tccagccggt gcatcaactt cggcagcagc
     3361 gcgtcttcca gcgcctgccg cagttgtgcg tcgctgtagc tcgcggccgg ctgcgggtag
     3421 gccagcgcgt cgcgcaggct gccgttcggg aagtaggggc gctgaggaat gaacatggtg
     3481 ttggccggca gacgggtaga gccgcggctg aagggccaga tgccggccag cgcgcgaaac
     3541 agcgtcgatt tgccgctgcc cgacgggccc ttgatcaaca cgttctcgcc ggcttcggca
     3601 tgcagcgaaa caccttccag caacaccgct ccggtgggca gcgccaaggt caggtcctgc
     3661 gtgtccaggc tggtggcgga ttcgtcggtt gctactgaat tcatagctgt ctgcgcttgc
     3721 cgcgcctggg ccagggccgt aaaactctct tcaaaactgg tgagacggtc ggttgtggcg
     3781 cgccaggcgg ccaggctgct gtagttgtcg acaaaccagc tcagcgagtc ctgcacacgg
     3841 ccgaaggccg acgagatctg catcaactgg ccgagctgga tggcaccgct gaaaaagcgc
     3901 ggggcggcca ccacaaaggg aaacaccacg gcggcctggc cgaagaaatt ggtgaaccag
     3961 atcaggtttt tctgtttctt gatcaaggtc aggtagttgc ccagcacggt ggaaaaacgg
     4021 ccttccaggt gcacgcgctc gaccgcctcg cccttgtcga gcgcaatcga ttcgctgtat
     4081 tcgcgcaccc gcaccaggtg gtggcggaag tcggcctcgt aacgctgctg caggaagttg
     4141 agccggatct gcggccggcc gatgtagtgc gtgatgatgc tgccggcggc gcagtacagc
     4201 accgccatcc agaccatgaa gccgggaatg ttgaagctgg tgccgcccag cgtgaagcca
     4261 aacggccccg acaggctcca caggatgccg acaaaactca gcagcgtcac cacggcgttg
     4321 agcaggccca tgctcaggga gatggtgtag gtggtgaaca ggttcaggtc ttcctggatg
     4381 cgctggtcgg ggttgtccgg tgcggccgtg ccattgccgc cattgccatt cgcgccatga
     4441 ctcgcataac gggccagctc catccggtag aaggcatggt cggccagcca gcgctgcagg
     4501 tagtgggccg tcatccaggc gcgccagcgt atctccagca gctgcgtcag gtaaaagcga
     4561 tagaccgcga tgatgatgaa ggcaaaggcc aggtaggtga agcgcccgag ctgctcccag
     4621 aacaccggct ggttcttgtc ctgcagcgcg tcgtagaaca cccggttcca ctcattgatc
     4681 agcaccagca tgtacaccgc gccgaggttg agcagcacga tggcgaccag cagcccgcgc
     4741 gccttccatt tgtcctcgga ttcgaagtag ggccgtgtca gcgcccaggc cttgcggcag
     4801 aaatccttga aggcggctaa cttgccgtgc acggcgggga ccaggggcgt caggggcatg
     4861 ggagtcatcc ttgggtgcgg aaatggaagc cagtgtagtg ccgcctgtcg cacgagtgaa
     4921 tggccgtagc gcgggaattc gctaggatgc cggcatgaac acctccacgc ccccttcccc
     4981 cgaaggcagc atagattgcg ccgtgcgcgg ccccttgctg ctgatcgcca tcaaccgccc
     5041 ggccaagcgc aacggcttca cgccgaagat gtttcgcgag ctgggccagg catacacccg
     5101 cctcgatgac gaccccgcct tgcgcgtggg cgtgctgcat gcgctgggcg atcacttcac
     5161 cgccgggctg gacctgccga cgattgcgcc gctgatgcag cgcggcgaaa aagccgtgcc
     5221 gctgggcctg gtcgatccgc tggacctggg catggaaggg taccggcgcc gcagcaaacc
     5281 catggtggtc gctgtgcagg gcatcaccta caccctgggc atcgagctga tgctggcggc
     5341 cgacattgtg gtggccgcag acgactgccg gttttcacag ctcgaggtca agcgcggcat
     5401 catggccacc ggcggcgcca cgctgcgcat ggccgaacgt gccggcctgg gcaatgcgct
     5461 gctgcacctg ttgaccggcg acgagtttgg cagtgccgaa gctctgcggc tgaacttcgt
     5521 gcagaaggtg gtgcccgccg gtcaggtcct ggacgaggca ttgcagattg ccgaggccat
     5581 tgccgcccaa gcgccgctgg cggtggtggc cacgcgaaaa aatgcactca aggcggtgga
     5641 acacggcccg gtgctggcca tgcaggactt catcgcggtg cagcaaggcc tgtcgcgcag
     5701 cgaagacgcg gctgaaggtg tgcgttcgtt tgtggaaaag cggccggcga agtttgtggg
     5761 gcgttaggct ttatctgtca tcccgggcgt gacccgggat ccatgccgca tgaactaccg
     5821 gcctgtcagt ctgcaggcat ggattgcgga tcaagtccgc aatgacaaac ctttggcaat
     5881 gcggtctcac gacagctggt tggacacgcc gctgaccaca tggccggaag gcacatgctg
     5941 cgaggctgaa tcgacatggc cggtctggtc gtcgaagaaa aagtcgggct cgaactcgcg
     6001 caaaaactcg cccttggcca gtccgccgag gaacatggct tcgtcaacct cgatgttcca
     6061 gtccatcagt gtgcggattg cgcgctcgtg cgccggcgcg ctgcgcgccg tgaccagcgc
     6121 cgtgcgtatg cgcatgcgcg aggtgccgac ctgctgcagg cggtgcagcg cttcgagcag
     6181 cggcttgaaa ggcccggcca gcagcggctg cgtggccttg tcgcgttcat gcgcctggaa
     6241 ggccgacagg ccctgcgcct ggaacacacg ttcggcctcg tcggaaaaaa tcaccgcgtc
     6301 gccgtcgaat gcgatgcgca cttcatgggg gtgctggtcg ctggcatggg ccgactgcgg
     6361 atagacctgc gcggccggca cgccggccgc cagcgcctcg cgcacatcgc tgaggtgggt
     6421 cgacaaaaac aggttggcat gcaggggttt gaggtagcgc cacggcggct cgccacggtt
     6481 gaaggtgccg cgctggatgg ccaggccgta atgctgggcc gagcggaaca cacgcatgcc
     6541 ggagaccggg tcgttgcgcg agaggatcac cacttcgacg cgttgtgcca gcttggccga
     6601 aggcgaggcg tcgtccaggc tgtcgggcgc gtcgttgaaa gccagcagct ttttgaccag
     6661 cgaaaaagcc acgccgggtt tggccggcac gtcgagccgg tccagctgca gctgcatgta
     6721 ggcggcgtca cgcttggtgc cgggcgcggg cggctggccc aggccgtcct cgaacacccg
     6781 gttttcttcc tcgaaatcaa acagcgcgcg cgatgaaatc gcgaccacca gttgtccgtc
     6841 cagagttgct gccatgggtt tacctgttta ctgcgggggt ggagtcaacg atagctcaag
     6901 tgtgcgccat tgtccgcagg ccacgctctc aagcggccac tggtcggccc gcgtgacctc
     6961 gcgcacgccg caggccagca gctgcgcggc gcggatgatg ccggcgtggg tgatccagac
     7021 ggtgtccggc ccggccggca gttcgtcgaa aacctgggcc acgcgcgcca tcacggcgcg
     7081 cacgctttcg ccgtggccgc cgacggcgtg gccggcaaag tcggccatcc aggcgtcgaa
     7141 gtcgctgcgc atgatgtcgc tccagcgccg gccttcccag gcaccgaagt tcatctcctg
     7201 cagccgggcg tcggtttgaa aggtcaaatc cggccgcagc ccaatcagcg cctgcgcaag
     7261 ttgctcacat ctttgtagcg gcgaggtgct gatctgcgcg ccagcgggaa gcacgtcggc
     7321 cagggaggca gcgctttgct ccgtcgcctc ccggtccgcc gccacatcca gcgcgccgta
     7381 acaggtgccc ggcgccagca ggacctgcgc gtggcgcacc agccagaggg tcatgccgtt
     7441 acaccgttcg ggctgagctt gtcgaagcct gtcctgagct cgacgcaggg cccttcgact
     7501 ggctcagggc gaacgggaaa aatgcctgcc aagctgacta caaggcgatc gccaggccga
     7561 ggtaaaaagc gatctcgcag acctgctgcg tggcgcccag gcagtcgccg gtgaaaccct
     7621 gcaggcggcg cgagaacagg cggcccatcc acagcaggcc gagcacggag aagctgcagg
     7681 ccactatcaa agtcatagct gaaagcgcat aactggcaag ggccagggcc gaaaatgacc
     7741 atacaaacgc caccgccacg ctggccagcg tgatctggtc ggccaggggt ttggacttgg
     7801 agcccgcggc atcgccgaca tgcggcagca cgcggatcag cagcagcggc agggtgcgcg
     7861 agaccacatg cgcggcaaac agggccgcgg cgaccagcca gccgtccagg ggcaggtcgg
     7921 gagcttcttc catgccggac agggcgcttt cgaccgagcc gagcagcgcc accagcgcca
     7981 ccttggccag cagtgcgagc accagcgcca tcgcgccgaa agcgccgacg cgtgagtcct
     8041 tcatgatctc cagggcgcgg tcccggtcgt aactgccacc cagcccgtcc gtcacgtcgg
     8101 ccagcccgtc ttcgtgaaag ccgccggtca gcagcaccgt tgccaccgtg gacaggacgg
     8161 cggccaccag cggtgcgaag tcggtgggtg gcagcagcgc catcagcagg ctgtacacgc
     8221 cggccgccac ggcgccgacc agccagccga tgccggggaa atgcgcggca ctggcgcgca
     8281 gcatggccgg gctgtagccg gtccacgccg ccagcgcgcc ggtgaccgga atgcgggtga
     8341 aaaactggac ggccagcagg tagtggcgaa tgaactggag catgtctaac tcttcccgct
     8401 gaccccggcc gaatcaaaac tcgccatctc tgccatgaac agcgctgccg attgcagcag
     8461 tggccaggcg agcagcgcgc ccgtgccttc gcccaggcgc atgtccaggt tgagcagcgg
     8521 cacagccttc aggtgggcca gcagcaggtg gtggccgcgc tcggccgagc ggtggcaaaa
     8581 caccaggtag tcctgcacct gcggcacgag ggcgcgcgcc accagcgcgg cggcaccggc
     8641 gataaagccg tcgaccagca ccacgcggcg ctcgctggcc gcctgcagca tggcgccggc
     8701 catcatggcg acttcgaaac cgcccagcgc cgccagcacg gccagcggct catgcacatc
     8761 ggcgtggcgg gccagggcgc gcgtgagcac cgcctgtttg tgggccagct gcgcatcgtc
     8821 caggccggtg ccgcgcccga ccacgtcgcc gatcggcagg ccggccagcc gggccagcag
     8881 cagggcggcg ggcgaggtgt tggcaatgcc catctcgcca aaggccacga cgttgccgga
     8941 caggccctgc atgacatcca tgccggcctg caacgcggcg tgggcctgcg cgagcgacat
     9001 ggcgggaccc tcgcacatgt tgttggtgcc cggggcaatt tttcgctgct gcagctgcgg
     9061 gtggtcggcc aggtcggcgg ccacgccggc gtccaccacc tgcagggcaa aaccgtgctg
     9121 gcgcgcaaac acattgatcg cggcgccgcc ggccagcatg ttgcccacca tctgcagcgt
     9181 cacggcctgc ggaaaggccg atacgccctc gctggcaatg ccgtggtcgg cggcaaacac
     9241 caccatttgc ggcgactgga acgtgatggc ctcgctgcgc tggatcaggc cgagctgcag
     9301 cgccagggct tcgagctggc cgagcgcgcc cagcggcttg gttttctggt cgatcttgtg
     9361 ctgtagccgg gcggccagcg cggcgttggc ggtcggttca atcgcgggca ggaaaaatgt
     9421 cttgtggtcg gtcatcggtg attttctgga ggcaggcggg gcaaaagcag gaggctcccg
     9481 ccgtgccggt tgtggatgat ggggtggacg gcaccggcat gatgtgaggc agcttgacgc
     9541 accagcattc cgtgtcccct cccaccatgc cgcagcgaaa agcggcaccg caacgggggc
     9601 atgatgaatt ggagggagcg gatgcgcctg ccgcagtcat ggccaggcct gtcagctttg
     9661 cagaaacagc cggtagaccg ggttgtcggt ctcttccaca tgcgggtagc ccagggtgtc
     9721 gagaaaggcc ttgaaagcct tgttgtcggc cttggggacc tgcaggccga ccaggatgcg
     9781 gccatagtcc gcgccctggt tgcggtagtg gaacagggag atgttccagc ccggtttcat
     9841 ggcgccgagg aacttcatca atgcccccgg ccgctcggga aatacaaaac gcagcaggcg
     9901 ttcgtttttg gacagcgcgg tgtgcccgcc caccatgtgg cggatgtgtt ccttggcaag
     9961 gtcgtcatgg gtcaggtcca gcgacttgaa tccgtgtttg ctgaagttgc cggcgatttt
    10021 tgtgctctcg cccttgccgt gggtggtcag gccgacaaac acatgggcct gactggcgtc
    10081 gctgatgcgg tagttgaact cggtcacgct gcgcgggccg cccggcagct cgccaatcag
    10141 ttcgcaaaag cgccggaagc tgccgcgctc ctcgggaatc gtcaccgcga acagcgcctc
    10201 gcgttcctcg ccgacctcgg cacgctcggc gacaaaacgc aggcggtcga aattcatgtt
    10261 ggcgccgcac aggatggccg cgtaggtctc gcctttggtc ttgtgggtag ccacgtactg
    10321 cttgatagcg gccacactca tggcgcccga gggctcaaca atgctgcgcg tgtcgatgaa
    10381 gacgtccttg atcgcggcac acacggcatc ggtgtccacc gtgatgaatt catcgaccag
    10441 ttcgcggctg atgcggaagg tttcctcgcc caccagcttg acggccgtgc cgtcggcgaa
    10501 caggccgacg tcgggcagcg tgacacgttt gccggcgccg accgattggg ccatcgcgtt
    10561 ggaatcgttc atctgcacgc cgatgatcct gacctcggga cgcacggcct tgatgtagtt
    10621 ggcgacgccg gaaatcagcc cgccgccgcc gatggccaca aacaccgcgt cgagcgagcc
    10681 tttgccgatg gtctggatct ggcgcaggat ttccatggcg atcgtgccct ggccggcaat
    10741 cacgtccggg tcgtcgaacg ggtgcacaaa ggtcaggccg ttttttttct ccagcgtgac
    10801 ggcatgcagg tgggcgtccg agtagctgtc gccgtgcagg atcacctcgc cgccgagcgc
    10861 cctgacggca tcgaccttga cctgcggcgt ggtcaccggc atcacgatca tcgcgcgtgc
    10921 gcccagcttg tgcgcgccga gtgccacgcc ctgcgcatgg ttgccggccg aggcgcagat
    10981 ga
//
LOCUS       DAIDFG010000077        10938 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_77_length_10938_cov_17.269687, whole genome shotgun sequence.
ACCESSION   DAIDFG010000077 DAIDFG010000000
VERSION     DAIDFG010000077.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 10938)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10938)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10938
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77_length_10938_cov_17.269687"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1989 a         3661 c         3462 g         1826 t
ORIGIN      
        1 ccaccgtgca gtcttcgacc gaccgcgcca ccgtgcgtgc acaagcggct gacgctgtcc
       61 gtctgggcca gatcccgcgc ggtgaagcca gctacatgta atagcggcgc tatctgcata
      121 actgagaatg ggccgggttt cggcccatcc aaaaccgtcc gggggcttgc tccggacggt
      181 ttttttgttc ttgcggaaac ccgccacccg gcgcgcaggg cgcggtgccg gcaccgtgca
      241 acaacgttgc ggcaaatgtc acaacgggtt aggtcggccg gcctactcaa tcacagggta
      301 atccctagaa ttcgataaca cgctcacgct tgaggaccgc gatccggtcc gtcagcgttt
      361 cctccgccct cccctgcggt ctacccgacc tcaggaatcc acaggttccc catggctctc
      421 ccctccattt ttgccgcgat gcggacacct cctgctgcgc cggacacccc ggaccggctg
      481 ccgcttgagc agatcaggca tcagctgcac caaaccctgc atgactgcaa gagtattgac
      541 gcgcagcgca tcatcttcag gatcaacacg gccgacaccc cggccgatct ctggctgttg
      601 cgcagtgacc tgtaccagtg catcgcgcgc gcacgcgacc agacagaagc tgccaggcgc
      661 atcaacgccc tgctgggcac tttcagcggc tggctgccag ctgcccagct caccccgatt
      721 taggacgacc ggtcccatcc cgcggaccat actcggctac gtgtaagcct gctgcccggg
      781 aggccatgtg gttctgaaaa aaaagcccgc tgcatggttg cagcgggctt ttttcgtggt
      841 tcaggcggtt cgcgcctttg agcccgtcac gggtaacgcc gcaccagaga ctcggccaca
      901 cacaccggcc tggtcgcacc ctcccgctcg accgtcactt cccaggtcat ctgctgcccc
      961 tcgttgtcaa tcgactcgca ggccagcagc ttcaggcggg cgcgcaagcg gctgccaaca
     1021 ggcaccggcg ccgtgaaacg cacccggttg agcccgtaat tgacgcccat gcgggattga
     1081 acgatctcca gggacgattc aaaaaatgcc ggcaacagcg acagggtcag gaagccgtgc
     1141 gcaatcggcc cgccgaacgg accggccctg gctttttccg ggtccacatg gatccactgg
     1201 tggtcgccgg tggcctgggc aaacaggttg acctgctcct gggtgatggt cagccagtcg
     1261 ctgatcgcga cttcctggcc gacacaggcc gccagttcgg agagggtctg gaaggttttc
     1321 atgcccggac tttaacgccc gcaccgatgg cggcctgtcc agccagcgac atggccgacc
     1381 gcggacgcag ccctgtcact ggtccagcca gcggcagatg ccctgccatt gctgcaggtc
     1441 tttttccacc cgccccgggg cgacgtcgaa cacggtcaag ccgcgggccg ccagatggat
     1501 gtagttctgg gtatcacgca cataaccgag taccggcaag cccaggcctt cgacaaaagc
     1561 acgcagtttg tcggccgcga tggtgcgggc atcgacgcgc atgccgacga tgccgacctg
     1621 catcttgccg gcgtggcggt gttcggcgag ctggtccaga aagctgcggg tggcaaaaat
     1681 atcaaacacg ctgggctgaa gcggcacgat caccttgtcg gccagcttga ggacgtcgtt
     1741 aaagcgccag ccgtgcaggc cggcgggggt gtcgagcacc acgtgggtcg tgcccttggg
     1801 cggtttgaca atcaggtcgg cgttgacatc ccaggtcgaa atcggccggg ctgcgggggg
     1861 ccgcagcccc agccagagcc gggacgactg ctggcggtcc gcatcgccga gcatgaccgc
     1921 atggccctgg ctggcgaaat aacctgcaat attggtcgac agcgtcgttt taccgacgcc
     1981 gcccttcgga ttggcaatca caaccacagg catgcgaatc tcccggaatt ttgacactat
     2041 ggtaacggct ggcaccccat cggccaaacc gcagacgggg cgggcgggtc gccatggggc
     2101 cgccccctct gcctgcgctg ctgccatgcc tgttcgtggc gccttgccat ttcagtcaaa
     2161 caacccctcc gcccttgata cgcgggcgga gaatgctcat gttcttatag caaaaaagcg
     2221 ggcacggcga tgctgcaagc cggcagcctc tcaaaacacc tcaattgcgc tggtcctgcg
     2281 cctcccgccg cagcttttcc atcgcgcgcc gttcgacaca gaccggatgc ctgctgtagg
     2341 caggtttggc gcattgttca ttcatgcaga tctgataccc aagcaacatg cggccttcac
     2401 aggcttgcgc cgggctggac ggctgcgccg gggcagcagc aggtggcgta acggcaaccg
     2461 tcgctgcagc gctcgtgccg gcgtccgggc ctgccgaggc cggcctggat ggcgccgcct
     2521 tgtccgtgcg cacgggggcg gcgcgtttgg gtgccggatg gacggcggca ggcgccgtgc
     2581 ccgccttggc tgaaggtgag ggttccgagg atcgcgccgc cgggctggca ggcgcggcgc
     2641 cggcagccgt ggccggcagg gccgcatcgg ctgtcgccgc aaccaccggc accggttcgc
     2701 cggacaagcc cttgaacacc acgccagcca gcaccagtac cgccgccccg gccagcgcga
     2761 acaggggcat ccgggagcgg cgtaccggcg ccgcaggcgt gcctgcctcg cccggcgcaa
     2821 ccggtgcacc cactggcggc gcaatcgtgc tggcagcagc gctggccggc gccggactgg
     2881 aaagcggcac cgtcgaaggc gcaccccagg tttcgtcagg acccccggcg tccggcccgg
     2941 ccggaacact tgctgcagga gcgggccggg tatctgtggc cggaatcggg gccgcccggg
     3001 aattgagaac ctgcaaacgc gtccattcgc tgacctcttc ggaaaaagcc cccgtggtgt
     3061 caggcccgct gccggtcccc gaaccatcga cctctaccgt gccgcccagt ttccgcaggc
     3121 gccgccgcgc gagatcggcg taaatgccgg acggaaacct gtcgagaaac ccttccaggt
     3181 cttccaggtc ctcggagtcc ttgatgtcct tccagtagac cagctcgagt tcctgcgtca
     3241 cggtcgagcc ggtgtcggtc ccgctcgtgc tgagcttgac ggtggagccg cccgcgcgtg
     3301 agcgcccggt gtccgtcgcc gtacctgcgc gcccgccggg cgggctcacg ggcggcacaa
     3361 tggtcgggtc cgaagcatgg ccgctggctt cctgcagcgc cgtcgaaaac tcctgcgccg
     3421 tcgcgaaacg ctggtcgcgt tttttggcca gtgcccgcgc catcaccgcg tcgatggcgg
     3481 gcggcaacaa cgtgttgaag ctgctgggct cggccggggt gtgacccaca atctgctgga
     3541 tgatcgcaaa gtcggtatcg ccgtcgaagg gccgcttgcc cgtgagcagc tggtacagca
     3601 ccacccccac cgcaaacagg tcggcgcgcg cgtcgatcgg caggccctgc acctgttcgg
     3661 gcgacatgta tttcagggta cccaccatgg ttccctgcgt gcgtgtggct tcaccggagt
     3721 cctgaatgcg ggccacgcca aaatcggtga gcttgacccg gccatcggcc tcgatcagca
     3781 ggttggccgg cttgacgtcg cgatgcacga tgttctgccg gtgcgcatag tccagcgcag
     3841 acaacaactc ccggaccatg cgcagtgttt cctccagcga ataacgccgg cccgcgtcca
     3901 gatggtgttt gaggtcttcg ccctggatga attccatgac cagaaaagcc atgtcaccat
     3961 ggcggtccga gtcatacaca ctgacgatat ggggatgctg caggcgggcc gccgaatggg
     4021 cctcggtgcg aaaacgcgct tcgtactcgg cggcggcctc ttccgacagg ttttccacct
     4081 tgatggtctt gatcgccacg cggcggttca ggttggggtc gcgcgcctcg tagaccagcc
     4141 ccatcgcgcc tttgcccagc acgcgaatga tgtcgtagcg gcccagcttg ttaagactca
     4201 tgggttaaaa aaagggggaa agaccagttg gatacgtttg gaaacagcaa ttctatcggc
     4261 ggccgcctca gctcctgata gaaaaactga ccaacgggct gccttccttg cgttcgcagc
     4321 cggcgctgat ctggccgctg ccgacgagat aacactcggt gcggcgcaac accacggctt
     4381 cgttctggtt gccgaagtag acccaggtgc cataacttga cgcatcgctg agcacgaact
     4441 gcccgccgcg ccattccagc gtggcgtgca tgcgggacac ccgcggatcg ttcaacgcca
     4501 gcgacgcccc ggcgccgcgg ccgatggaca tcttcgcgct gccggcattg acttgcacgg
     4561 cctgctcgcc ggccaccagt tcgagaacca tgggccgggc atcggaggcc atcgaccgcc
     4621 ccatcatggt ggcatcgtcg tcgcggccgc tttgccattc cacccggtac accaggctgg
     4681 cttccgcctt gccgcgcagg tgcatgggcc ccaggctgcg caatgatgcc cgctgcacag
     4741 gccccagggc cgaccagacg ttctgcgtgg tgagaatctg cgccgcacca gccaggtcgg
     4801 ccaggcgcgc cgcagaattg acggtatcgc caaaacagtc gccgtcgatc tcgatcacat
     4861 cacccgactc catgcccatc tgcatctgca ccggcgcgcc agtgccgccg ggccggatgg
     4921 gtttgtcgag gaagcgcttc tggatggcga cacaggcggc cagcgcatca ccctcctgcg
     4981 gaaacacgac aaacaacccg tcgcccagca gcttgacgac acggccgttg tgctgctcga
     5041 agacctggct cagcgccccc acgagctgcg tgacaaagcg cgaggcggtt tcatccccca
     5101 gccgctcgaa catgctggtg ctaccgacga gatcggcaaa aacgacggtg gtcatgaaga
     5161 cattctggaa aaccggaggg cagaacagac cggtacgcac gtggtgtcaa acggtaacac
     5221 gtaacctttt gttctcatat tggcgatttt tacagaaaat cacccgcccc cgcgtgtgaa
     5281 gtttttacca gcgaagcgcg ccacccgcag ggcggtgtcg cctggcgtca gcgcaaaccg
     5341 tcccagagca gcttgcagcc tgtgaggaac atgccgccgt acagcacccg gtaaaacagc
     5401 agctggctga tgcgcctggc catgcgcaca ccagcccaca cgcccacggg cgccagcggc
     5461 agcagggcca gcgaggtcgc catgttgcgc aggtcgagca gccccagcca ggcataggga
     5521 atccatttgg agaggttgat cacaaagaag aaaaacgcca gggtggcggt gaatttgagc
     5581 gggctcaggc gcaggggaat gacataggca ctgatgggcg ggccgccggc atgggcgaca
     5641 aaactggtga agcccgaggt cgcggtcaac aacgcaccca gccacttcgg cggcggcggg
     5701 ctgtcgggcc ggggcggaaa aagcaggcgc tgcgccagaa acagcagggt gaaaaaaccc
     5761 acgatgccgg ccacgacctt gccgtcgagc aggctgaaca aaagggcgcc gatcagcgtg
     5821 cccaccaggc cgcaaggcag caggaacttc agcagcttga ggtcgaagtc cttgcggaac
     5881 gccgccatgc cgagcaggtc catcagcagc aacaccggca tcaggatggc cgccgcctgc
     5941 ggcacggtca ccgccagcgc catcatgggc acggccagcg agccgaaacc cgccccgaac
     6001 ccgcttttgc tgacccccag caacagcacg gcgggcaccg ccacggcgta aaagaagggg
     6061 tcggtgatga gcggaaaggt cacgggtttg caggtgaaag ctgccgatta tcaggcctgc
     6121 atggatgacg gcgccaccgg gctgcatcgc ccgcagatcc gccgcgggtc agtgttgatg
     6181 cagagaatcg gcctccagcc cttgagggac aggcgcaagc agctattatt tcagtagcat
     6241 cgcccgactg caggggccgg cgatttcatt cgtgcgtctc caggcccaga caggctttct
     6301 ggatttccgg gcgtgtcagc agctcgccgt ccacatccat gttgtcattc atgggcggtc
     6361 tcctactgtt gcggggtggc gggcggactt tcgtagtcga cctggtggcg cgcgctgcgc
     6421 agaccggcga gttcgccctt cacgcgcagg tgttcggggt ggccggcata ggcatcaagc
     6481 gccgcctggc tgtcgaactc cgaataaagc accacgtcgc aggcgtaatc gacctggctg
     6541 gcatcgacac cgatttccag gtgcgcgagg ccgggaattt tcccgcgcag gctttcaaaa
     6601 ctgcgctgca gcctggcgat gcccgccgcc ctggcctcgg gcgtgtcgcc gcggatgttc
     6661 cacatgacga tgtgtttgat catgcgggca acccggcctt gtgctcgggc cggacgcgct
     6721 ggcgcagttc gccactgtcg ggcttgtcga tcagtgaaac gatgtcaatc acgtcatgcg
     6781 gccgggttga ggacaaaatc aaaatcaagg cggtacacac cgtcgggctg cttgacccag
     6841 tcggccacca gcgactggcg cacgccgaac accgcatcgg agtcgaggta agggtccccc
     6901 ttgcgaaaca cgtgggtcac cagcgtttca taagccgggg ccttgatcat gaaatgcagg
     6961 tgcgctggcc gccaggggtg gcactttgtc gcccgcaaca tgtcgccgac cgggccgtcg
     7021 acgggaatcg ggtaaggctc ggccacgatg gttttaaagt tgaaactgcc gtcggcaccg
     7081 gacctgagca cgccgcgcgc ctgaaacttg tccaggtcgg cgtactgcac atcgtagtgg
     7141 ccctcggcat cggcttgcca gacctcgatt tcggcgccgg ccaccgcctc gccgcccagg
     7201 cccagcaccc ggccgcgcac cacgcagggc tcaccgaccg cgccgttggc cacatcggcg
     7261 ccgttgtcga aatgcggcgc gccctcgaca tgaaaggggc cgaacaccgt ggcctcggtg
     7321 cagccctgcg gcttgtcgtt gttcatggcc acggtcagca tggacaggcc cagcgtgtcg
     7381 ctgagcagaa tgaactcctg acgcttgtcg tcacatttcc ggccggtggc ggtgaggaag
     7441 tcgacgcctg cgagccactc ggcttccgtc aatttcacct cgcgcgcaaa tgcgtgcagg
     7501 tgctgcacca ggctggtcat gagttccttc aggcgcgcat cgggcgtgcc ggcgaatcgc
     7561 gcaatcacgg cctgggtgat ggtgtcctgg ttcaggttgc gcatgacggg gccggggcaa
     7621 ggagggggaa atggatatac atactttcct tcgtgaacgc tctggtccaa atgtaggtca
     7681 aggcgcccct ggagaaaagc ggactccgca aatgactttt ccgcaccgcg gaaagacggc
     7741 agcagcggct gggccacaat gcaacgacct ttaaaaaaca caagcggcaa ctgcaggaga
     7801 catggcgtgg atcgctggac ggagattgaa ctgtttgtgc aggtggccga aaccggcagt
     7861 ctgggccgtg ccgccgaagt gctggagctg tccaatgccg ccgcgagccg gcacctgtcc
     7921 gcgctggaag agcgcctggg cgcccggctg gtggaacgca acacgcgccg gctctacctc
     7981 accgacaccg gccaggagtt tttcagccgc gccaaaacca tcctgtcgga cctcaaggat
     8041 gccgagtcgg ccgtcaacgc caccgcgctg aacccgaccg gcatgctgcg catcacggcc
     8101 tcgctgtcgt tttctctgca ccacgtggcg ccgctgctgc gcgagtacac gcagcgctac
     8161 cccaacgtga cggtgcatgt cgaggccgcc aaccgctacc tggacatcat cgacaacaac
     8221 atcgacgtgg ccatccgcac ccgcgagacc gagcccgact ccaacatcac gattcggcgc
     8281 ctggccgaaa cgcgtcgcat cctgacggcg tcgccgcgtt attttgcgca gcacggtttt
     8341 ccgaaaaccc tggaagacct gcacaaacac aagctgctga tctacaccta cgccaacaac
     8401 cccaacgagc tgcgcttcac gcgcgacggt aaaaccacca ccgtcacggt caagggcctg
     8461 ctggagtcca atgacggtca gatcctgcgc accgcggcgc tcgacggcat gggcatcctg
     8521 gtgcagccca gctacatcgt ctacaacgac atcgtggccg gccgcctggt gccggtgctc
     8581 gacgactggg acctgccgca cctgaccatc aacctcgcct accccagccg caaacacctg
     8641 tccgccaagg tgcgcacctt catcgacttc atggccgaac atttcgcgaa gcaggactac
     8701 gagcgcaaat ggaccggacg cttcggggtg tattgagatg gcaaacgttt tgcgtaccga
     8761 cgtgctgatc gccggcggcg gcccctgcgg cctgatgctg gccaacgagc tcggccgccg
     8821 cggcatagcc tgcctgctgg tggaccccaa acccggcacc gccttcaacc cgcaggccaa
     8881 cgccacgcag gcgcgcacca tggagcattt tcgccgcctg gggtttgccg gcgagatccg
     8941 tgcccagggc ctgccggacg accacccgac cgacattgcc tacttcacgc gttacaccgg
     9001 ccatgagctg gcccgcctgc acctgcccac cgccgcgcag gcggtgaaaa acatcaaggc
     9061 catgagcggc tcctggagcg cggccgagct gccgcatcga atatcgcaga agtttgtcga
     9121 ggccagcttg cgccgccacg ccgaagctct gcccggcgtc gatgtgcgtt acggctgggg
     9181 ccttgaaagt ttcgaggacc gcggcagcca tgtgcaggcg caggtgcgcg ccaccgccgg
     9241 cggcgagtcc ctggacgtgc aggccgcgta cctggtcggc gccgacggcc cccgcagcct
     9301 ggtgcgcagc acgctgggca tcgcctggga cggcgccagc ggtgtcaggc gcgagttcat
     9361 gggcggcaag atgtttgcgg tgtatctgcg cgcgccggat ttttacagcg tcttcccgca
     9421 cgcccgcgcc tggatgtacg tgtcggtcaa cccgcagcgc cgcgccttca tggcctcggt
     9481 ggatgggcag tcggagtttg cctttcacgc cgccgtgcac gagggtgaag atgccgacgc
     9541 ctggaccgcc gccgacgcac agcggattgt tgatgaagcc atgggcatgc atctggccat
     9601 cgaggtcttg tcggtcggca cctggacggc cgggcattcg ctggtggcgc aaagcttcca
     9661 gcgcggccgg gtcttcatag gcggcgacgc ggcccacctg ttcacgccga ccggcggcct
     9721 cggctacaac accgcggtgg aagacgccgt caacctcggc tggaaactgg ccgccgtgct
     9781 gcgcgaccag gcaccgccgc agttgctgga cagttacggg ccggagcgcc aaccgctggc
     9841 cgaacgcaac accgcctatg cccggcgttt cgccgattcg gtcggcagct tccgtgcctc
     9901 cccgctgctg gagcaagagg atgccgacgg ccgggccgag cgcgaacgcg cctccagcta
     9961 tctgaaccag cacgtgcgca tggagttcaa cattcccggc gtgaccttcg gcgggcgcta
    10021 cgacgcctcg cccctgatcg tgaaagacgg cagcacagcg ccgccggacg aagccggcag
    10081 ctaccagccc agcgccagcc ccggtggccg cgcgccgcac gcgtggctgg ccgacggccg
    10141 ctcgctgtac gacagtttca acttcgaatg gacgctgctg gccctggggc cggatgcgcc
    10201 cggcacccgc ggttttgtgg acgcggcggc ggcgcttggc ctggacctcc aggttgtcgc
    10261 gcacgtatcg gaaacactgc gggcgctgta cgaagcgccg ctggccctga tccgccccga
    10321 ccagatcgtg gcctggcgcg gcagcaacag ccggcaggcc ctgcaggtgc tgaagcaagc
    10381 cagcgggcac gccgccggca ccttctgaca gcatcgacta ctcctgattt gatagctggt
    10441 tgcgcccatc tgataagggc tggcgctcga tttgactgaa cctttttggc agcgccaccg
    10501 gttcaccggc ctatcaccgc aagataagct tggcgtgtct ccgggtccac ggatgccaac
    10561 atctgctggt aaggcgtctg gtgccgggcc agcatgcggg ccgaggcgac cgccctggag
    10621 tggcaatacc agtggcaggt gtgctgcatc aacaacagct cggccgacag ggtgtaggcc
    10681 ttgtcacgcg gcgcgcggcc ctgtgcattt tttgccgcgc gctccatccc ctcggcatga
    10741 acgaccagga tgcgccgcat gtcgaaggtg ttgtcgggga aaaatcggtc ggtctcaggc
    10801 agaaacgccg gcagcttgct gacgcgggca tcggcttcgt ccaccgccga aaaatccgcg
    10861 gtaaaacgcc ggaactgttc acacaactgc tcctcggaga aagccagcgt ggcccagagc
    10921 cgggtctggc gctccggg
//
LOCUS       DAIDFG010000078        10701 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_78_length_10701_cov_17.311854, whole genome shotgun sequence.
ACCESSION   DAIDFG010000078 DAIDFG010000000
VERSION     DAIDFG010000078.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 10701)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10701)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10701
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78_length_10701_cov_17.311854"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1912 a         3320 c         3503 g         1966 t
ORIGIN      
        1 gccgaccagg gcgcgacagc catggcccag gccatcgagt cggtggaggc cgtgcagggc
       61 agcgccaaac gcatgggcga catcgtgggg gtgatcgacg ggttggcgtt ccagaccaac
      121 atcctggcgc tcaatgcagc ggtggaggcg gcgcgtgccg gtgagtccgg ccgcggcttt
      181 gcggtggtgg ccggcgaagt ccgttcgctg gcgcaacgtt cggccgactc ggccagggaa
      241 atccgccagc tgatcggcgt gtcgtcgggc caggtggcgg cgagtgcgga aaaaatccgt
      301 gcggcgggtt cgaacatgaa ccagatcgtc tcgggcatcc gcgacgtggc ggccaacatg
      361 gcgcagatct cggcctccag tgccgagcag agcaccgggc tgaccgaaat cacctcggcc
      421 gtgcaccagc tcgacgagat cacccagagc aatgcccgga tggtggggcg cgcagtcacc
      481 caggcgggca acctggaggc gcgggccggc acgctggtgc agtcggtggc gtctttcaag
      541 ctgcagcagg ggtcggccga agaggcggtg gcgcttgtgg cgcgcgccgt ggcgcatcgc
      601 cggcacacct cgcgagacgc cttcctgcgc gacctgaccc tgcccgccag ggggttccac
      661 gatcgcgaca tgtatgtttt tgcgctcgac agccagggca cctacctggc cttcggcggc
      721 aacccgtcca agattggcac acgggtgcag gacatccccg gcatcgatgg cgggcaattg
      781 ctccagtcca tcatcgacca ggccagccat gagccgggct gggtggagta cggcatcacc
      841 aacccgacca cgggcaaggt gcaggccaag atgtcctttg tccagcaggt ggacgatgtg
      901 ttcgtgggct gcggcgtcta taaaaacctg gtggcgagct gataacgaca aaacggaact
      961 gagggattca cccgagggcg ctgtgcgccg ggccgggcgt aaactggtcc ggcctcctga
     1021 cccggtggtg ttgcagcggg gcgaaggaga cagcgttttg cagccaacca acgtagccca
     1081 tccgtcctac ttccacaagg tcgtcgattg ccaatgggcc tgtcccgccc acacccccgt
     1141 tcctgaatac atacgcctga tcggccaggg tcgttacacc gacgcctaca tggtcaactg
     1201 ggtgtccaac gtcttccccg gcattctggg ccgcacctgc gaccggccct gcgagccggc
     1261 ttgccgcagg ggccgcgtcg aggagaacaa cggtgagcag ccggagccgg tggccatctg
     1321 ccgcctcaaa cgggtggccg ccgacatgaa aaacgatgtc cgccagcgca tgcccccgct
     1381 ggcgcccaaa aatggcaagc gcatcgcctg catcggtgcc ggcccggcct cgctgacggt
     1441 ggcgcgtgat ctggcgcccc tcggttatga ggttacggtg tttgacggcg aggccaaggc
     1501 cggtgggttc atccgcacgc agatcccgcg ttttcgcctg cccgaatcgg tgatcgacga
     1561 agaaaccggc tacatcctgg acctgggcgt gaacttcaaa agcggccagc gcatcgagtc
     1621 catgaaaggc ctgctggccc agggctacga cgcggtgttt gtcggctgcg gcgcgccgcg
     1681 cgggcgcgac ctgaagctgc cgggccgtca ggagatcacg gaaaacatcc acatcggcat
     1741 cgactggctg gcctcggtct ctttcggcca catcaccacg attggacaac gcgtgattgt
     1801 gcttggcggc ggcaacacgg ccatggactg ctgccgttcg gccaggcgcc tgggcggcac
     1861 cgacgtgaag gtgatcgtgc gcagcggctt cgacgaaatg aaggcctccc cctgggagaa
     1921 agaagacgcc cagcacgagg gcattcccat catcaacttc catgtgccca ggtcttttga
     1981 acaccgccag ggcaagctca ccggcatgac ttttgaagtg gtccaggcgg tgtacgacgg
     2041 caacggcaaa cgcagcctgg tgccgacagg cgagcccgat gtgctggtcg aatgcgactc
     2101 ggtgctgatc gccgtgggcc aggaaaacgc ctttccctgg atagagcgcg agagcggcat
     2161 cacgtttgac gagtggggct tgccggtcct gaacaaggag actttccagt ccagcttgcc
     2221 gtctgtcttt ttcggcggcg attccgcctt cggtcccaaa aacatcatca cggcggtggc
     2281 gcacgggcat gaggctgcgg tgtcgatcga ccgctttctg aacggcgagg acatccgccg
     2341 ccggcccgac ccgatgacca atctgatgtc caccaagatg ggcatccatg agtggagtta
     2401 cgacaacgac acctccaacg atgcccgttt caaggtgccc tgggccaagg cggaaattgc
     2461 gctggcgagc atacgggtcg aggtggagct cggtttcgac gccgcaacgg ccttcaagga
     2521 agccgggcgc tgcctgaact gtgatgtaca gacggtgttc gctgcgccgg cctgcatcga
     2581 atgtgacgcc tgtgtcgata tctgcccgat ggactgcatc agcttcaccg tcaatggcga
     2641 cgaggccgat ttgcgcacgc ggctgaaggc accggcgctg aacctggcgc aggacctcta
     2701 tgtgtcgggc gaactcaaga ccgggcgggt gatggtcaag gacgaggatg tctgcctgca
     2761 ttgcggcctg tgtgccgagc gctgcccgac cggggcctgg gacatgcaga aatttttgct
     2821 gcagaccacg caggccgggc catcctgtcg cgatgccaac aaggctgcca gccgcgccgc
     2881 aggagtgccc gcatgaaaaa acgaattgaa gccgtcaacg actttgtgat caagttcgcc
     2941 aacgtcaacg gttcgggctc ggcctcggcc aacgagctgt ttgccaaggc cattttgcgc
     3001 atgggggtgc cggtgagccc gcgcaatatt ttccccagca acatccaggg cctgccgacc
     3061 tggtacgagg cgcgtgtgag tgacaagggc taccagggcc ggcgcggtgg cgtcgacatg
     3121 atggtggcca tgaacccgca gacctgggac gcggatgtgg ccgagctgga accgggcggc
     3181 tacctgtttt acgacagcac gcgggccctg ccggcgtcca gcttccgcga cgacatccat
     3241 gtcatcggca tgccgctgac cgagatctgc aacgcggtct acagcgaccc gcgccagcgc
     3301 cagctgttca agaacatcgt gtatgtgggc gcgctgtcgg tgctgctgga gatggagccc
     3361 gaggtgcttg aaaagctgtt cggtgagcag tacaagggca aggaaaagct gctcgcctcc
     3421 aacgtgcagg ccctgcacct gggccgggac ttcgccagcg agcacctgca ggcgccgctg
     3481 ggcatacgcg tgcagcgccg cgacagggtc ggcaacagga tttttgtcga tggcaacagc
     3541 gccgcggcgc tgggctgcgt ttatggcggc gccaccgtgg ccgcctggta cccgatcaca
     3601 ccgtcctcct cggtggccga ggctttccag tcctattgcg ccaaataccg ggtggacccc
     3661 gagaccggcc gacacaacta cgccgtggtg caggccgaag acgaaattgc ctccatcggc
     3721 atggtggtgg gcgcgggctg gaacggcgcg cgcgccttca ccgccacctc ggggcccggc
     3781 gtgtcgctga tgaccgagtt cctcgggctg gcctattttg ccgagatccc tgtcaccatc
     3841 atcaatgtgc agcgcggcgg gccgtccacc ggcatgccga cccgcaccca gcaggccgac
     3901 ctgatctcct gcgcctatgc ctcgcacggc gacaccaaac atgtgctgct gctgccggaa
     3961 gacccgcacg agtgttttga acatgcggcg gcggcgctgg acttggccga ccggctgcag
     4021 acgccggtgt ttgtgatgac cgacctcgac atcggcatga accagcgcct gtgcgaaccc
     4081 tttgcctggg acgacagccg cagctatgac cgcggcaagg tgatgacggc cgaggagctg
     4141 gaggccggca aggacttcgg ccgctacaag gatgtggacg gcgacggcat cccctggcgc
     4201 accttgccgg gcacgcaccc cagcaagggc agctatttca cgcgcggcac cacgcgcaat
     4261 gcctatgccc agtattccga gcgcggcccg gactacatct acaacgtgca gcggctgctg
     4321 aaaaagttcg agaccgcggc cacgctggtg ccgcagccgg tgttgcggcc cgcggcgcgc
     4381 ccgacccggc tgggcgtgat ttatttcgga tccaccagcc cggccatgaa cgaggcgctc
     4441 gatgtactga ccgaggccgg catccacatt gatgcgctgc gtctgcgggc ctttccgttt
     4501 cctgcatctg tggcgcagtt catcgccgcg catgacaagg tgtttgtggt cgagcagaac
     4561 cgcgatgcac agatgcattg cctgctggtc aacgaactcg atgtcgatcc ggcgcgcctg
     4621 gtgcgggtgc tgcattacga cggcacgccg atcaccgcgc gtttcatcac cggtgccatc
     4681 acgcagcagg tgcaggccgg ggcggcggca caacaagtat ccagggagct gtcatgacct
     4741 atctggccaa accgcggctg catcatcctt cgctgaccgt caacaaggtc ggctacaccc
     4801 ggcgggacta cgaaggcaag atttccacgc tgtgcgccgg ctgcgggcac gactcgatct
     4861 cggcggccat catccaggcc tgttgggaac tcgacattgc accgcaccgc gtcgccaaac
     4921 tttcgggcat aggctgcagc tccaagacgc cggactattt tctcggtgcg tcgcacggct
     4981 tcaacaccgt gcatggccgc atgccctcag tgctgaccgg cgccaacctg gccaaccgcg
     5041 acctgctgta cctcggtgtc tcaggggacg gtgactcggc gtcgatcggc ctgggccagt
     5101 tcgcccacgc gatgcggcgc ggcgtcaaca tggtgtatct cgtcgagaac aacggcgtgt
     5161 acggcctgac caaggggcag ttttcggcga cggcggacca gggctccaaa agcaagaaag
     5221 gcgtggtcaa cagcgacagc ccggtggacc tggtagccct ggcgctgcag ttgggcgcga
     5281 cctacgtggc gcgcgggttt tcgggtgaca aggcgcagct ggtgccgctg atcaagggag
     5341 ccatcggtca tggcggtgcc gccttcatcg acatcatcag tccctgcgtg gccttcaaca
     5401 accatgccgg cagcaccaag agttacgact acgtgcgcga acacaacgaa gccgtcagcc
     5461 gcatcgactt catcagcgcg cgcgaggaaa tcaccaccag ctacgctccc ggcgaggtga
     5521 tcgacgtggt gcagcacgac ggctccagcc tgcgttttcg caagctgcac gccgactacg
     5581 atcccaccga ccgttatgcg gccctgagtt acatgaacgc gcatcaggcg cgtggcgaag
     5641 tcgtgaccgg gctgctttat gtcgacccgc tggcgaccga cctgcatgtg gcgctcaaca
     5701 ccagcgatac agcgctgaac aagctggatg ctccggccct gtgtccgggg gcgcgtgtgc
     5761 tggacaaggt caattcatcc ctgcgttaaa ccgactcgct ggacaggagg accctcatgt
     5821 ctgaacgtac cgtgtttcaa tccatcccgc gccggcatgt ggtgagttgc ccgccgcagg
     5881 ccagcgtctg gcaggctgcc tgtgtgatga ccaaggccaa ctgcggcagc gtgctgatca
     5941 ttgacgctgc cggcgtcatg cagggcatcc tgaccgagcg tgacctgatg acccgcgtgc
     6001 tggccaaggc cctgaatgcc aaaaaaacgc cggtgtccga ggtgatgaca cacaacccgt
     6061 tgtgtgtggt gcccgagctg accgtgtcgg atgccgtgtt gatcctgctg gagcgcggat
     6121 tccggcattt gccgattgtt tcgtcgggcg gaaaaatcct gggggtgttc tcgatccgtg
     6181 atgccttgcc gcgcgagatt gacagcgctg tcagcctggc cgagttccgg gactcggtca
     6241 atgacgcgct gggctgagag agcaggctag ctgttgcctg cgtcgcgtct ggcgcgcgag
     6301 cgtgccagcg cggcttcaat gatggcgcgt ttctggtcca gcaccaccgg gtccgtgtgt
     6361 tgtgaatgcc cgggccagtc tgccagcgcg gcctcggcct tttcttcaag ccttttacgg
     6421 ttttttccct cgtccagaat gcgtcgatag ctatgaattt catagcgttt gcgggccagc
     6481 ccggcgtcgg cttccgacca tgcagcccag ccggtacgct cccccgtgac gttttccagg
     6541 ctgatgcaat ccacggggca gaccggaatg cataactcac aaccggtgca gcaggactcg
     6601 atcaccgtgt gcatgagttt gttgctgccc atgatggcgt ccaccgggca ggcgtccagg
     6661 cacagggtgc agcctatgca ccagtcctcg tcgatgacgg ccatctggcg cgggccttcg
     6721 ctgccgttgt cggggttgag ggggagggcg ggccggccgg tgatcagggc caggcgcgca
     6781 atgccttcgg cgccgccggg cgggcactgg ttgatcccgg cctcgccatc ggccagggcc
     6841 tgcgcgtagg ccgcgcagtc gggatagccg cagcgtgtgc actgcgtttg cggcagtgcg
     6901 gcattgagcc gctcggcgag caggttcacg acacggcgct caggatcgat ggtcgcttac
     6961 tttttggcag cgctcttttt ggcgacgctg cgggtagctg gcttgggcgc caccttgacg
     7021 gctggtttgg ctacaggctt ggctgccttc tttggagttg ccttgacctg tgcggaaagg
     7081 gtgggcccct ggttgtgctc aagaatgaaa gccttgactt gcggatacac gatgtcgcgc
     7141 cagcggcggc cgctgaaaat gccgtagtgg cccgcgccct tgacttccag gtgcttctgg
     7201 cgtgccttgg gcacgttgta gcaaaggccg tgggccgctt cggtctggcc ggagccggaa
     7261 atgtcgtcga gttcgccttc gaccgtgaag tgggccgtgg tggtgatgtc ctcggggcgc
     7321 acgcgctcga gcttgccttg cctggacttc acgtcccagg tgccgttgac cagcttgaac
     7381 tcctggaaca cggtcttgat ggtgtccagg tagtagtcgg cgtccatgtc cagcacggcg
     7441 ttgtattcgt cgtagaaccg ggtgtgggct tcggtgctgg cgtcgtcgcc cttgatcagg
     7501 tccttgaagt agtcatagtg gctggtggcg tggcggtcgg ggttcattgc gacaaagccg
     7561 gtgtgctgca aaaagcccgg gtagacacgg cgaccggcgc ctgcgaagtt gctcggcacg
     7621 cgatagatca cgttgttttc gaaccattcg taactcttgt tcatggccag gttgttgacg
     7681 gccgtcggcg atttgcgcgc gtcgatgggc ccgcccatca tggtcatgga cagcggcgtg
     7741 gtttcgcccc ggctggccat cagcgaaatg gcggccagca ccggcacggt cggctggcac
     7801 acgctgatca cgtggcagtt gccgtagatg ccctgcacgt gggtgatgaa ttcctgcacg
     7861 tagttgacgt agtcgtcgag cgcgaaggca ccgtcggaca gcggcacggt gcgggcgttt
     7921 ttccagtcgg tgatgtagac cttgtggtcc ttgagcatgg tcttgacggt gtcgcgcagc
     7981 aaggtggcgt agtggcccga cagcggcgcc acgatcagca cggccggctg gcccttgagt
     8041 ttggtcagag tggcaggatc atcggtgaaa cgcttgaagc ggcgcagctc gcaaaagggc
     8101 ttgtccacct cgatgcgttc atgcacggcc acgccgacgc cgtcgacatc caccgtggtc
     8161 aggccgaagg ccggtttttc gtagtccttg ccgaggcggt aaatcaggct gtaaccggcg
     8221 gccgcccgct gcgccagcgg gctttggctc aagggtgacg tcgggttgtt gtagagcttg
     8281 gcggcggcct gggcgaagtc gacaaacggc tccatcagcg aacgctgggt ttcataaact
     8341 tgataaagca tgggctgacc tttgtacaac gatatgctgc agtgcagcaa ataataacag
     8401 gtgtgcttac gattaaatgg agggcaaaca ccaatacgga agggcctgaa gacacttctt
     8461 tttgccgttc aatggaaaat ctcacatgtc tggccgctgg atgcctgaag attgtcccgg
     8521 caggccggcg aaaaaacctc aacaaagcca cgcaaagcgc cagggccgcc ggccctggct
     8581 tgtacctgac ggatgtttat gaccgagacc accagcgccc tggcgcttga cgacttcact
     8641 tcactggaaa aatcggaccg gatgccggtg ttgtttgtcg gccatggcag ccccatgaac
     8701 gcgattggcg acaacgaata ccgccgtagc tggcaggaac tgggcgcagc gttcggcagc
     8761 cagtacccgc cgccgcaact ggtgttgtgc atttcagccc attggctgac cgaaggctgg
     8821 tgggtgaccg gcatggcgca acccaggacc atccacgatt tcggcggttt tccgcaggag
     8881 ctgttcgatc agcagtaccc ggcgccgggc gcacccgccg ccgcacgcga gatcagccgt
     8941 tttgtcaggc agcgcgcttc ggccccgctg ggggtggacc tcgacgcctg ggggctggac
     9001 catggcgcct ggtcggtgct ccagcccatg tttccggcag cggacattcc cgtggtgcag
     9061 ctgagcatgg actacagccg cccgccctcc gaccactacg cgctggcgaa acagctcaag
     9121 ggcctgcgcg agcgtggtgt gctgatcgtg ggcagcggca acatcgtgca caacctgcgc
     9181 caggcgcagc gcggcgcggc cggccaccag gcctatgatt gggcgctgga gtttgaccag
     9241 accaccgggg gctacattca acagggcaac ctggacgcgc tgcagaactt ccagaagctg
     9301 ggccagctgg cgctgatggc gcacccgacc catgaacatt acctgccgct gctgtatgcg
     9361 gcagcggcgg tggacagcag tgaagcaccg cgttttttca acaccagttt ccagttcggc
     9421 tcgatctcca tgcgctccgt gatctggggc tgaaccagga acctacccac accatgccaa
     9481 gccgccaaac tgtccaggac ttcatcacac tggtggagca gggccaatac gtcgaggcca
     9541 tcgagcagtt ttatgcgccc cacgccagca tgcaggaaaa cagccagccg ccccggcgcg
     9601 gccgcgacgc gctggtgacc tacgagcggg ccgtcatggc cgcctgcagt gcgatgcgca
     9661 ccttgccggt ggactccttc ctggtcgacg gcgacttcgt cgtgatccac tgggtctttg
     9721 agttcacgcg tgtctcaggc tcgatgctgc gcatcgacga gctggcgcac cagcgctggg
     9781 agggcgacaa gattgtggaa gagcgctttt tctacgatcc gacaacggtt ggcgcctgat
     9841 acctggctga aatggctgcc acggctgtca ctatcaaata gatagctgct tgcgcttgtc
     9901 ccataagggt tacaggcact tttttcataa attattgaag gctgccgcga gtggcgccgg
     9961 gtcgggccgg ctcagccatt gccagcgtgc ctggtagtcc cgcaccttgt tttcatacag
    10021 gggcccatgg tccacacgtg ggttggggct caagatgccc cggtacatgt cgtccagccc
    10081 atagggcgcg taaacctgta tgtagccggc ttcccggcgc aagccgacgc tggtgctgcc
    10141 caccagaaaa cggtccacgc cttcgcaggc gtttttcagc ggcaggcagg gccgcccgaa
    10201 atacgccggg taccaggtgt gcacacgcgc ctggttcctg acgtcgagct ggacgtccag
    10261 gcccgaaaaa agcgcctgcg cctgccggtt gacggcttgc tccgcctcac cggaaaggtc
    10321 cagcgggtcg aagtagaaaa catcgtagtc gcggatacct gcgccggggg ctcggccgtc
    10381 cagcaggttc cagacggtct ggaacaggca acctccgacc agccaggcgt cgggcaactg
    10441 cagctcggcc aggccgtcca gcagggcctg gttgcagggg ttgcgcaggg catcctgcac
    10501 caaccgctgc ggcaggcctg ctccgccgat ttacatcacc ttggcgatgg acgcgcagac
    10561 gtgggcgatg ttgtggctgt tcagcgcggc cacacacatg cggccggtgt cggtgccgta
    10621 cacgccgaat tcattgcgca ggcgcaccat ctggtctttc gtcaggccgg agtagctgaa
    10681 catgccgatc tgcttggtga t
//
LOCUS       DAIDFG010000079        10620 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_79_length_10620_cov_16.970469, whole genome shotgun sequence.
ACCESSION   DAIDFG010000079 DAIDFG010000000
VERSION     DAIDFG010000079.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 10620)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10620)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10620
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79_length_10620_cov_16.970469"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1938 a         3216 c         3417 g         2049 t
ORIGIN      
        1 aataaacgca gcaggtcgca aagtcgggct gtgttcgggc tataatattc gactagtcgg
       61 agtgtggcgc agtctggtag cgcacctggt ttgggaccag ggggtccaag gttcgaatcc
      121 ttgtactccg accaagaatc caatgaaaaa gctcactctg tgggcttttt tcatatctgc
      181 ccgtagctca gttggataga gcatcagcct tctaagctga gggtcggggg ttcgatcccc
      241 tccgggcaga ccagtgctga atgcgctgcg tccgccaacc ctccccattt ttattgttct
      301 gaccgtctca ggcgcagcct tgcgtccgtg cgcggtgcct tgccgcgccc atgcgttgcg
      361 aaatcacgtc gctgtgctcg cttggctcga cgtggatata ggcccaaagt catagagcca
      421 ccccagcgaa ttgtcaaaaa ttcatccttt agcataaaaa aagttaactt gttaaaaagt
      481 caccaatgga atcgcaattt tttctacaaa agtcacattt agataaatag aatcgcataa
      541 aagatacaaa aaactctgcc ggagggacaa aaagggcagt ccggcatcct ggaaatgcag
      601 ctttcccact tccgccatac ggcgggcgtc tggtcaggct gatgcatgtt gacgttgatt
      661 cattaacaaa ggggtgatca tgaaatcaga gcatttttcg acattcaagc ggcgtgcatt
      721 gcacacaaca ctggcatccg ccctggttct gggaatggcg gggcacggca tcaactcgca
      781 ggccgcaacg acctcgaacg atgccaccgc aacggtggtg gcgccgatcg ccatcgccgc
      841 cggcacaacc ctgcgtttcg gatcgttttc caccagcgca gccgcacaga cggtgaccat
      901 caacgcaacg agtggtgcgc gaaccaacag cggtacgctg ctcgtcacct cgacggtggg
      961 tcgggcaacg ttcaacgtca ccggcgaagg aacgctgact tatgcgatta ccctgccgac
     1021 caacggcacg gtgaacatca cgaccggtgc agcgtctgca cccgaaacca tggcggtcag
     1081 caacttcacc agcgacccgt ccggtacagg cgcattgact gccggcgcac agacgctggg
     1141 ggtaggggca accatcacca ccgtcggcag tcaggtggct gggtcgtaca ccggaagctt
     1201 caacgtttcg gttgattaca actaagcagt ctgcgattcc cggcaggccg ggccagcccg
     1261 cgaacgttcc aggcgactga aagcggtgtg gccggcaagc cggtctggtt tccgggaccg
     1321 tggtcccaca acacttggcc tcgtattcca acggggttgt cgagaaatgc ctgcgatgaa
     1381 aacgctccgc ctcctgtccg cgctggcctg cgcggccgct atcgcgtcca cgggttgggc
     1441 gcagacgctg ggggccaacc aggcactggc ctttggcagc tttgctcccg gcgccagcag
     1501 tggaacggtg accatcagcg ccgccggtgc ccgcagttca gggggcggcg tttttctggt
     1561 gccttccggt gccggatccg ctgcgtcgta caccgtgagc ggtaccagca gtgcgaccta
     1621 cgccattacc ttgccatcga acggtgtggt ggtgttgacg agtggcccga attccatggc
     1681 cgtcaacacc ttcacgagca atccgggggg aaccggcctg attggtggcg gtggaactca
     1741 gacattgacc gtcggggcca cgctcaccgt tggaagcaac caggccaccg gcagctacag
     1801 cggcagtttc gacgtcatgg ttgagtacaa ctgacaagcc acgcttcatg aaaaaacgag
     1861 gaaccccggc atgacaccct ccacttttat tgcccgttca gtgccagcag gtgccgcagg
     1921 ggtgttggtg ctgcggatcc gcttcaccct gcgatgcctt ggctggctcg cgggggtgtg
     1981 cttcattttc ctgactgcgc cgcctgcatc ggcagacctg atgctgtttc ccacacgtgt
     2041 cgtgtttgaa aaaaaccagc gggcggccca gttggagttg atcaacaatg gacaggagag
     2101 caccacgtac cgtgtccatc tggtgaatcg gcgcatgaac gaaaccggtg agttcaccgc
     2161 ggcagaacct gctttgcccg gtgagcagtt tgccgatgcc ttgttgcgtt attcgccgcg
     2221 gcaggtggtg ctggcccctg gtcgcggcca gaccatccgc atcctgttgc gcaaaccggc
     2281 agacctgccg gtgggtgaat accgctcaca tctgcagttt gatctggttc ccgaggccac
     2341 cggcgccagc agcattgacg cccaggcgac caagccgggg ccgggcgaag tcggtgtcca
     2401 gctgagggcg ctcgtgggcg tatcgattcc tgtgatcgtg cggcacggtg aaacgtcggc
     2461 catcgtctcg ctgtccgggc tggaactgcc caggcccgtg gctggccagc ctgcgatgct
     2521 ggccgtggtg ttgcagcgca gcggcaaccg ttctgtgtat ggggatctga ttgcaacgtt
     2581 cactccacag ggcggcgtgc cgcaggaagt cggaaaagcc ggtggcgtgg ccgtttacac
     2641 gcccaatgcc ttgcgccgcg tcaggctggc gctacagccg ccagccggag tggtgctcgc
     2701 acgcggcacc ttgcgtgtga gctatcggga gcgtcctgac gcgggtggca agttgctggc
     2761 ggaagccgcc atcgaattgc cctgaccggg acagcggttg cgcccaccca tgacgcagca
     2821 gctttcatca tgaggggcca cgctttattt cgacgccttg ccggcggggt ttcgatgcgt
     2881 agctgcagga tccggctgct tctcccggtg gcgctggtgc tggcgccggg catggcgttc
     2941 gccagcatct tggtcagcgc agcatcagtg gaaaggggtg atgcccggac gcgcatcgtg
     3001 cttgagtccc ggacggagct gcgcttcagt ctgtatatcc tgcgccatcc cgggcgggtg
     3061 gtgctggagc tcgaagaggt ggcgctcaat gcggcgctga ccggactgac gggccagatc
     3121 gctgccgatc acccctacat gcaacctatc caggtcaggt catccccttc cggtaccgga
     3181 ccggttctgc tggaattggg actcaaggca gaggccgaac ccaacatttt ttcgctcaag
     3241 cccgaagcgg gcaggggtta caggctggtg ctggacatcg caccatccgt ggcacctgca
     3301 cctgcacctg cggcgcctcc ggcagcgccc ccggcccgcc agcagccggt gctggcggcc
     3361 gcaacggcgc ccccagcgcc tccagcggca attcccgggc ccctgcgccg cgccgatgag
     3421 ttgaacctgg tattgctcga ggtgcaactc gatgaacatg tgctgaccga tgccatcacg
     3481 acctaccagg ctggccgcga gacatttttg cccctgggcg aactggccag gctgctgacg
     3541 ctggcgatcc ggacgcaggc cgaacagggc acggccgacg gttatgtgct gagcgaggct
     3601 cggggtttca gcctggatac ggcccagggc aggatcacgc tggccgacca gacccagctg
     3661 tttgaccgga cgctggtccg tcagcagggc gacgacatct acgtcgcggc ttcgctgctt
     3721 tcccggtggt tgccgcttga tttcgaggtg gacctgtcca gcctgacact gcgggtcaag
     3781 cccagggaac gccttccctt gcaggagcgc ctggagcgtg agcgccgcgc aggcaagctg
     3841 ggcgcccgcg ccggttacga agacccgggc tacccacgcg tggacatgcc ttaccgcgtg
     3901 ctgggcgtgc cttttatcga ccagaccttc gggatgggcc tgcgtaccgg caatggcagc
     3961 cggcagacca gcgccagcta caccgcctac ctgacgggcg acctgctcgg cctggaatcg
     4021 gcattttttg tcagcagcac ccgccaggat ccgtcgccgg acgtgcgttt cacactggca
     4081 cgccacgatc cggatgcggg cctgcttggc cccatgcgcg cccggtcggt gatgttcggc
     4141 agcggtgttt ccgccccgag tgtgacccac atcgccggcc gcagtgcaac cggcaagggt
     4201 tatgggctga cgctcagcaa ccgaccgatc acccagccga caagttttga tcgccatacg
     4261 ctgcagggtg atttgccgcc cggttgggat gtggagctgt atttcaacga ggcgctggtc
     4321 ggttttcaga gctcgcgtgc ggacggccgc tacagctttg acgaccagcc cctggcctat
     4381 ggtctcaatg aatttcgcct ggtgtttcac ggaccgctcg gccagcaacg ggtggagcgc
     4441 cagagttttt tgctcgagca atccagcacg ccgcccggtg cgttttatta cagcctcgcc
     4501 cagcaccagg acagcgccgg ccagtcgcgt tcggtcgccc agtttgagtg gggcctgaac
     4561 aagtacctga ccgggaccgc tggttttgtg cgcttgccct catcggtgac ggccggccca
     4621 accaccggca tcaacactgc ggatggacag ctgtacagca acctggggct gcgtggtttc
     4681 tggaaatccg ccattctcag caatgatttt ttccgctcgc ccaatggcgg ctggctcaat
     4741 gagtcaggcc tgaagacccg tgtcgggcct gtctcactga gttacagcca cctgcggctc
     4801 aaggacttct acagcgagct gtttttaccc actaccgatc ccttgcgcac acgcgacaag
     4861 ttgcggctgg acggggccat tccggcaggc tggttgccgc gcttgccggt gaccgtggaa
     4921 gtccagcgcg agcagtttca atccggtctc cgcaacgtgg ccctgaccgg ccgcgtcgcc
     4981 gcctatgtca agggcacctc ggtggccaat cagttgacct ggcagaactc gggtggtacg
     5041 accacggcca gcggtgccct gcaactgagc tatcgcgtcg gggtcaccgg tctgagtggc
     5101 cagttgggct acctgctcaa acccgaggca aaactcgata ccgttgtgct ttcgggcgac
     5161 aggcggctgg gcgaaggcta cctgctcaat ctcggcgtgg tgcgctccat cagcagccgg
     5221 gagacactgt acacggcagg cctgaacaaa agcctgggca gttacgggct gggactgagc
     5281 accagctatt ccagcacggg ggtgttgacc ttgggcgtgc aactctttat tgccatgggc
     5341 agggaaccac gccagggaca gtggcgtttt gacgcgctgc ccaaagccga cagcggtgca
     5401 gcatcggcgc gtgtttttgt ggacagcaat gccaacggag ccatggacgc tggcgaagaa
     5461 gcggttgaaa acgccgcgat caccgtcaac ggcagccggg cgccggtgcg gaccgatgcc
     5521 gcggggattg cctggctgga ccgtttgccg acgcggcaac atgtggacct ggcggtcgac
     5581 acgcagacac tggaggatcc ctactgggcg ccgcagcgca agggggtgcg tgtggtgcca
     5641 cgccccgggc atgtggcgga aattgatttc cccatcattt tgaccagcga aatagatggc
     5701 acggtgtacc tggtcgagaa caatacacgg cgcggcatcg gcgatgttgt gatcgaattg
     5761 cttgacagcc accggcgcgt ggtgaataca atcaaaacca gttcagacgg ttattacatt
     5821 gttccggcca tcccccaggg ccgctaccat gtgcaggtct ctctggagca actgaggcgg
     5881 ttcggtctgg tcgatccggg tgtgcgtgag atcaacatcc tgcccgacgg aaaattcatc
     5941 aacggcgtgg attttctgct gcgaaaaatg ccggccggag cggagtccgg tcttccccgc
     6001 gaggagcacc atgaaaaaaa atgattggcg gccaaaaaaa cctcagccgg gtattgcctg
     6061 cgtgttcatg atcggtgggt tgatcctgat gaccggcagc agccatgcgg tgacggcggc
     6121 aggcatcgcc agcgcaacgg tggtcggctc ggtggcggtc attgtcacgg tgcccgcgcc
     6181 tgcacctgta gcgacacccg tagtaacacc cgccccagcg cctgcggtgg ttgaaacgcc
     6241 cctggccttg ccggggacgg tcttcacggt cgaaagtttc acaagcagcc tgtccaccag
     6301 tggccccttg ctgcgtgtcg ggggggcgcc tcccgccggc gccggtggaa gcggcgctca
     6361 gggaggcggg gcggcggaag gtggagccgc agcacagggc gccacggctg cacagggcag
     6421 ctcgggcgca ggtgccgcgc cgccagcggc cggtaccggg gccgtcaatg tgacgcgcaa
     6481 ggccgatggc tccctggccg tcagcggtgg ttcagggttg acctttgccg tgtcgcaacc
     6541 ggtcaacggc gcggtcaaca tcgagtacaa ctgactcggc tgcagctgcg cgtctagcgt
     6601 ttctggtatt gcgtcttgcc aaacagcact tcgcgtgcct tgtcgtcggt gatggggcgg
     6661 cgcagatcgg caagcacctt gacgccgcgc tgcacggcag gccgggccgc gatgacgtca
     6721 aaccatttct tcaggtgcgg gtagtcggcc cagtcgatgc cgtggttttc ccagttgcgc
     6781 agccacggaa agatggcgat gtcggcaatc gtgtactggt tgcaggcgat gaacttgctg
     6841 cccgccagct gctggttcat cacaccgtaa agccgtcggg tctcgttggt gtagcggttg
     6901 atcgcgtaat cgattttctc gggcgcgtac tggcggaagt ggtgggtctg tccgatcatg
     6961 gggccgacgc cgcccatctg gaacatcagc cattgcagca cctggaactt caaccggtca
     7021 cttttcggca ggaatctgcc ggttttggcc gccaggtaca gcaggatggc accggattcg
     7081 aacagtgcca tgggtttgcc gtctggcccc tcggggtcca ccagcgcggg aatcttgttg
     7141 ttcgggctga tggccagaaa ctcgggctgg aactggtcgc cggtgccgat gttgaccgga
     7201 atcgcttccc agtcgcgacc gaggcggaag ccgcactctt ccagcatgat gtggaccttg
     7261 tggccgttgg gcgtgggcca ggaatagact ttgagcatga acgcaacctt tggaactatg
     7321 atggcaaagt gcttgtccgc gccccttgcg ggcaggcaga caagttctca agcactttaa
     7381 gagaaaagcc ggaatcatgt ttgagcgcct gaaaaaggtc tttgtgcgag gcgagactca
     7441 gccgctgcaa gcggcaaggc ccgacgcggt gtcggagtgg gccagcacgc atggcttcag
     7501 ttactccagc cagggcaagg gcgccggttg tgtgctgacc ggcaaggtga aaggcaagcc
     7561 ctggaagctg gagagcgggg cggcatcacg cgactacatc gtcggcgagg aactccgagc
     7621 ccgtgcagcg ctggacatca acgaagatgc ggcggtgctc atcatgaacc ggccgctcaa
     7681 ggagtcgctg gaaaaacgcg cctacgcgat ttacaccgac acgctgcaga ccacggccga
     7741 ccccagcctg cccgaggaga tgcgctggct cgcgatgtat gacgaagttg gctggagcag
     7801 cgtggtgcca gaattctggg aacgttacac cgtgctggcg gaccagcgcg aacatgccct
     7861 ggcatggctg gacggcagcc tggccgaact gctgctccac tggcccgagc cgggcccgga
     7921 tgcgcagacc cccttcatcc tgatgctgct ccgcggcaaa gcctacctgc gcatgcagta
     7981 cagcccattc agcatcgcca cgctgcaaca tgcagccatc gtgtacacca gcgcctgtga
     8041 atcggcgctg gccggcctgt cgacggacat tcccctttga ttcaagccag aaatggcctg
     8101 agcccaataa ccgcggacgc gagcagctat attttttata gctgatcagc gccgggtttt
     8161 tcctgtcagc tgccgcgggt gatcggcatc tcgacctcgc cgggcatggc caggtgtttg
     8221 gccagttcca gcttggctag cgaagcgcgg tgcacttcat cagggccgtc ggccagacgc
     8281 agcgtgcgct ggtgggcgta ggcataggcc agcgggaagt catcggacac gccgccaccg
     8341 ccatgcgcct ggatggccca gtcaatgatc tggcaagcca tgttgggcgc gaccaccttg
     8401 atcatggcga tttcggcctt ggcgaccttg ttgccgaccg tgtccatcat gtaggcggct
     8461 ttcagtgtca gcaggcgggc ctggtcgatc atgcagcgtg cgtcggcgat gcgctcgtgc
     8521 cagaccgact ggcgtgccac cggcttgccg aaagcgatgc gggtgttcag gcgcttgcac
     8581 atcagttcaa gtgcgcgttc ggcaatgccg atgctgcgca tgcagtggtg aatgcgtccg
     8641 gggccaaggc ggccttgcgc aatttcaaaa ccgcggcctt cgccgagcag caggttgccc
     8701 accggcacgc gcacattggt cagccgcact tccatgtggc cgtgcggtgc gtcgtcgtag
     8761 ccgaacacgc tcagggggcg gatcacggtg atgccgggcg cattggccgg caccaggatc
     8821 atggactgct gctcatggcg gcccgcgtcg gggttggttt tgcccatgac gatgtaaatt
     8881 gcgcagcgtg ggtcaccggc acccgacgac caccatttca ggccgttgat cacatagtcg
     8941 tcaccgtcgc ggacgatgct gcactggatg ttggtggcgt cggacgaagc cacttcgggc
     9001 tcggtcatca ggaaggccga acggatctcg cccttgagca ggggttccag ccagcggtcc
     9061 ttgttggctt ccgagccata gcgggcgatc gtttccatgt tgccggtgtc gggcgccgag
     9121 cagttgaaga tttcgggcgc aaaaggcacg cggcccatga tttcgcacag cggcgcgtat
     9181 tcgaggttgg acaggccctc gggtgcccgc ggcgagtgcg gcaggaacag gttccacaag
     9241 cccgccgcgc gggccttggg tttgagttcc tcgatgatgg ttgtcggaat ccacgggttg
     9301 cccttggcgc ggttctctgc aatctcgcgg aagaaacgcg cctcattcgg gtaaacatgc
     9361 tggtccatga aggccagcag gcgggcctgc atgtcctgga ccttgggggt gaagttgaat
     9421 tccatgtagc tatctcctgg gtaaaggttt cttttagccg ggcttgtggc ccgactggct
     9481 tttttgcgca aagtcccagg ccatgcgcgc cagcgcgggc gcgcctttgg cagaggcgac
     9541 ggcctgtgcg ctggccgcgg tgccggcttc gacgcgcttg gcgatgccct gcaaaatggc
     9601 cgcgagccgg aacaggttgt aggcgaggta aaaattccag tcggccttga tcagttccgg
     9661 gctggcaaag ccagtgcgtt cgcaatacag gcggatgtac tcagcctcgg tcgggatgcc
     9721 gaggctggcc acgtccaggc cgccgatgcc gcggaaagca ccgggcggaa tgtgccaggc
     9781 catgcagtga tagctgaagt cggccagcgg atggcccaag gtcgagagtt cccagtccag
     9841 cacggccagc acacgaggct cggtcgggtg aaacatcagg ttgtcgagcc ggaagtcgcc
     9901 atgcacgatg gaaaccatgg attcatcgcg ggcgctggcc ggaatgttct cgggcagcca
     9961 tttcatcagc gcgtccatct cggggatggg ctcggtgatg gaggcggtgt actgcttgct
    10021 ccagcgaccg atctggcgct cgaaataatt gccgggcttg ccgtagtcgg ccagcccgcg
    10081 ctcggcgaac ttcaccttgt gcagcgcggc aatgacgcgg ttcatctccc ggtaaatatc
    10141 gccgcgctgg gcattcgtca tgcccggcaa ggcctggtcc cacaggacac ggccctcgac
    10201 aaactccatc acgtagaagg cgcggcctat cacggcctcg tcttcacaca ggcagtacat
    10261 tttggcgacc ggcacgtcgg tgccctgcag gccggtcatg accttgaact cgcgctcgac
    10321 cgcatgggcc gatggcagca gtttggcgac cggccccggc ttggcgcgca tcacgtacga
    10381 gcgctggggc gtgatcagct tgtaggtcgg gttggactgg ccgcccttga aaatttcggc
    10441 gctcagcgga ccctggaaac ccggcagatg cttctcgagg taggcactca gcgcttccat
    10501 gtcgaaagcg tgctggcccg acaccgcgcg tgtgcccagg aagtggtcaa agttgctcat
    10561 cgctttattt ttccaggtcg gcttcagaaa tgcgcatcag cgagatcgga agagcgtcgt
//
LOCUS       DAIDFG010000080        10540 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_80_length_10540_cov_16.863424, whole genome shotgun sequence.
ACCESSION   DAIDFG010000080 DAIDFG010000000
VERSION     DAIDFG010000080.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 10540)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10540)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10540
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80_length_10540_cov_16.863424"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1880 a         3536 c         3307 g         1817 t
ORIGIN      
        1 gcaggtagtg gcggatcttg gtggccacat ccccttcgac gatctggcag tggtacaggt
       61 ccatctgcac cttgaggttg gacgcgccga cctcggtcac gatctcgtgc gcatggtcct
      121 gccggttcaa aaagaaacac ggcacatcgc gcgtgttgat cggctcgatc aacacgtcgc
      181 gcccgtgttt cgccgcttcg gccgccgccc agcgcaggtt ggccacatag accggccgca
      241 ggtcctcgcg ctcccgccct gcggcgacca ggccggccat caggtgaata cgcgggcaat
      301 ccagcgcctc ggcataggcc agggccagca acacaccggc gcggaactcc gcctcgcggc
      361 ccggcacggc ggccgtgccc ttgtcgccgg cctggtccca ggcctgggcg atggatgctg
      421 catcggtgcc gcccggtggc gcgttgaaca gcacctgctg caggccgttg cccttgaggc
      481 gcgcggccag ttcatccttg ctgtaggcat aaggaaaaag aaactccacg gccttgaaac
      541 cgtctttcgc ggccgcctcg aagcggtcca gaaacggcaa ctcggggtac atcatggaca
      601 ggttggcggc gaatttaggc atggtggtgt tttaaaatac tatgaaatag atagctgcca
      661 gcgcttatga ataaagggct acaggccaat ttgacaataa aaagcctacc agcgggcgcc
      721 gaaggtctgg cgcagttcgt cgatctgcgc agcgcttaaa gcttgcaccg gggcgtcgtc
      781 caggcacagc agccgggcgg tttcctcgag ttcttccagc acggccatgg ccgccgccgg
      841 actgtggtgc cagacattgg gccccagccg cgccagcatc accgcgcgta gcggcgtgcc
      901 ctgccggccg tagcgcgtga tggcctgggc caccagcgcc gccgccgccg ggtcgcccgg
      961 gcggtggtag tcgatgaccg gcacatggcc aaccttcatg acgaaataag gcgtgagcgc
     1021 cggcagcaac tcctcgcccg tggcgcgcag gctcagcgcc acgcaatggg tgctgtgggt
     1081 gtggatcacg cagcgcgtcg ccgggtcaaa gacggaggct gccgcgtaaa tgccggcatg
     1141 cagcgcgatg gtcttgctcg cggtgtcgcc gctgagctgc tttgcatcgg cgtccagtcg
     1201 cgccagccgc gccgggtcca gaaaacccag gcaggcatcg gtcggcgtga tcaggaaacc
     1261 accgccctcg gaagcgtcga gccgcacgct gatgttgccg gcggtggcgt gtacataacc
     1321 acgctcgaac aggcttttgc cgacgcggca gatctcttcc cgggcctggt tttcggtcaa
     1381 tgcagtcatg ccagcatcgt aaaggctttg gtgaagaagt cgtcggtgcc gaagttgccg
     1441 gatttcaggg tcaggtgcaa accatcgctg cctgtgattt caggcagtgc atgacaccag
     1501 ggcacaccag gatcgatctg cgggccgatc tgcatctgcg tgatgcccag tgcctgcacg
     1561 caggcgcccg aggtttcacc gccggccacc accagttgcc gcacacccag ctgcaccaga
     1621 ccgcgcgcca ctgcggccag tgtggcttcg accatggcgc cggcttcttc caccccgagc
     1681 ctgccctgca cggccttcac atcgccgctg tcggcggtgg aatacaccag caccggcccc
     1741 tgcgccagca gcggccgggc ccaggccagc acctcggccg ccacatcggc acccgaggca
     1801 atgcgcagcg gatcaatcgc cagcgccgga tggccctgct tcatgaagtc cagcacctgc
     1861 cgattcgtcg ccagcgagca gctgcccgac accaccgcct gcaggccgcc ggcggccggc
     1921 aaacggctgg cctgcagcga cggcgtgatg ccgaagttgg ccggcaggcc aatggccacg
     1981 ccggaaccgg cagtcaccag tggcatgccc ttgagcgcag ggcccaggcg cagcaggtcc
     2041 tcgttggaca ccgcgtccac aaccgccacg cccacccctt ctgcctgcag ttgcgccatg
     2101 cgctcgcgca ccgccgccgc gccgcgcgcc acgaccttgt ggtcgatcag gccgacacgc
     2161 cgctccgtct gcgactgcag cacccgcacc agattcgcgt cgaccatcgg cgtcagcggg
     2221 tggttctgca tgccgctttc gctcagcagc acgtcgccca caaacagata acccttgaac
     2281 accgtgcgct ggttgtcggg aaaggccggt gtggcgatcg tgaagccggt gttcagcgcg
     2341 tccatcagcg cctcggtcac cgggccaatg ttgccgcggg ccgtgctgtc gaaggtcgag
     2401 cagtacttga aataaatctg ctgcgcgccc tgcgcctgca gccaggccag ggcggccagc
     2461 gattcggcaa tggcctggac tgccgacaca gtgcgtgatt tgagtgcgac caccacggcg
     2521 tcgacctcgg cgcccagcgg cccggagggc acgccaatcg tctgcaccac acgcatgccg
     2581 gcgcgcacga ggttgttggc cagatcggtc gcaccggtga aatcgtcggc aatacatccg
     2641 aggctgatct tgctcatggc tttttcggca gctcgatgcc cgggaaaatt ttgatcaccg
     2701 cgctgtcgtc ctctttcgca aaaccggccg tcgaggcctg catgaacatc tggtgcgccg
     2761 tcgaggacag tggcaaagga aacttgctgg cgcgcgccat gtcgagcacg atgccgaggt
     2821 ccttcacgaa aatgtcgacc gccgacagcg gcgtgtagtc ggccgccagc acatgagcca
     2881 tgcggttttc gaacatccag ctgttgccgg cgctgtgcgt gatgacctcg tagagcgcgg
     2941 ccgggtccac cccttcacgc aggcccagcg ccatggcttc ggcagccgcg gcaatgtgta
     3001 cgcccgccag cagctggttg atgatcttga ccttgctgcc ggcgcccgcc gcttcaccga
     3061 gcttgtagac cttggcagcc atggcgttga gcacaggttc ggccttcgca tacgctgccg
     3121 gcgcaccgga ggtcatcatg gtcatctcgc cgctggcggc tttggcggca ccaccggaaa
     3181 tcggggcatc gatgtaaagc aggcccaggt cggccaggcg tttttcctgg gccatggacc
     3241 agttcgcgtc caccgtggag cacatcacaa acacgctgcc gggtttcatg gccgccgcac
     3301 aaccgccggg gccgtacaac acggactcgg tctgcgccgc attgaccacc accgacacga
     3361 tcacgtcaca ggccgccgcc agctcggcca gcgaggcgca ggccacaccg ccaccttcgg
     3421 caaaagcctg cgccaccccg ggccgcacgt caaacacaaa cacccgataa ccggcgcggc
     3481 gcagcgattg cgccatgccg ctgcccatgg caccgagacc gatcacacct gctacaagac
     3541 tcatcccctt gctccttcag cccaggtcat ttttttgaaa tccgccacac ccattcacgt
     3601 cgccacgccg gccgccatca agcagcccat ggcttgcaga tgccataagg ccgacatgac
     3661 ccagaatgaa cgacttattt atatggtcat catacaactt gcacaccatc catcacctca
     3721 ggttttcccg aatcgagaca aggcgaacgg ctcagtgccg gatggcgtcg gaaagaaggg
     3781 cactggccag ctgcacaccc tcctgctgcc agaaagccgg gtcggcctgc tcgatgcgcc
     3841 ggatcgcgtt gtccatgtgg cgggcggcag cctggcgcgc cagcagggcg tcgccggcct
     3901 cgatggcctg ggtgatggct tcgtgctcct cgcgcacctg cccggcaaag tcggcgcggc
     3961 gcgcctcgtt ggcgcgggtg acccgcgtga cgccgcgcag gaactggcgc aggtagtcca
     4021 gcgtgctgat cagaaacggg ttttgcgccg cgtcggcaat cgcgcggtgg tagttcacgt
     4081 cttcgtcggc accgtcgccg ccggccttga ccgccttgtc gagcgcggac atcgcctggc
     4141 ggatgcactt gatatcggcc gccgtgcggc gctgcgcggc caacgcggcg acctcggcct
     4201 ccagcgcgcg gcgcacctcc accatctgga tgaccgcctg catggacgcg gcagaacggg
     4261 tgtcgaaatt cagcggcgca aaggcggccc gcttcacgaa caccccgctg ccctggcgcg
     4321 actccaccag gcccagcgac ttcaggcggg agacagcctc gcgcaccacc gtgcggctga
     4381 ccgcgaactg ctcgaccagc gcggcctcgg tcggcagctt gtcgcccgcc gccggccggc
     4441 cggcgcggat ttcagccgac agggcctcgg ccacctgttc cgagaggtga acgccgggcg
     4501 tgatggactg gaagcggtgg ctcatgatga tccggaaaat ttgtcgtaca actctacaac
     4561 atccggcagc cgcttccggg ggttaaaaca ccagggtctt gacgccgacg tccgtgccca
     4621 gcaggcagac atccgccttc tggaaggcaa acaccccgac cgtcaccacg ccgggccact
     4681 ggttgacctc ggactcgaag gccagcggct cgctgatctt caggccggtc acgtccagaa
     4741 tgtgctggcc gttgtcggtg accagcggcg caccgttttt caggcgcagg gtcgccgtgc
     4801 cgccgagggc ttcaaactgg cgacggatgc gctgcgccgc catcggaatg acttccaccg
     4861 gcaaggggaa ctggcccagc acctgcacca gcttggactc gtcggcgatg cagacaaact
     4921 tgcgtgactg ggcggccacg attttttcgc gcgtcagcgc tgcgccgcca cccttgatca
     4981 tgtagcccgc gtggtcgatc tcgtcggcgc cgtcgatgta gaccgccagt tcctcgacat
     5041 cggccgcctc aaacaccgtg atgcctagcg ctttcaggcg ctcggtggag gccaccgagc
     5101 tcgacaccgc gccggcaatc gtgtccttga tgccggccag cgcgtcgatg aacttgttga
     5161 cggtggagcc ggtgccgaca ccgacaatgc tgccgggttc gacataggcc agggcagcct
     5221 ggccaaccag tgttttgagt tcgtcttggg tcatgtcggt ccgcaatgaa agggggtgga
     5281 tggggtttgc gacaatgggc tgtcgcacag aacaactagg gcctgtttac actatttata
     5341 caagatgcgt tgcgagtcaa aaaggtcatg cgtgaggcgc aaaatgcagc cgggctggag
     5401 cccggcaagg ctttgcaacg ctacgcatgg cctttttggc cgcaacccgg agggaaggtg
     5461 gtgaaaacaa cgccactcgt tgttgcactc ctagcccagc ccgccaggct gagcgtcgtc
     5521 gcgcgcctag attggcgctg ttttcaccac cttcgcactt gcataaatag tgcaaacagg
     5581 ccctgaatta tccaatgtcc ctgctcccct acgccctcgc ccgccccttt ttatttggtc
     5641 tggacccgga aaccgcccat gaactgacca tggcctcgct ggccggcacg cagcgcacgc
     5701 cgctggcact ggcctactgt tcgggccggg tggacgatcc cgtcacgctg gccggcctga
     5761 catttcccaa ccgtgtcgga ctggcggccg ggctcgacaa aaacgcacgc tgcatcgacg
     5821 ggctggccgc catgggtttc ggctttgtcg aagtcggcac cgtcacgccg aaagcacagc
     5881 cgggcaaccc caagccgcgc atgttccgcc tgccgcaggc caacgcactg atcaaccggc
     5941 tcggcttcaa caacgacggg ctggacgcct tcctcaccaa tgtgcagaag tccggttttc
     6001 gcaagcagaa ggtcaagcac cctctgctgc tgggcctgaa catcggcaag aatgccgcca
     6061 caccgatcga gaacgcggtg gacgactacc tgatctgcct cgatggcgtg tacccgcatg
     6121 ccgactacgt gacggtcaac atctccagcc ccaacaccaa gaacctgcgt gcgctgcaaa
     6181 gcgacgaggc gctggacgcc ttgctgggcc gcattgccga acgccgcgaa accctcgccc
     6241 ggcagcatgg caaacacgtg ccgatttttg tgaagattgc gcccgacctc gacgaggcgc
     6301 aggtcggggt gattgccgcc accctgaaac gccacggcat ggacggtgtg gtcgccacca
     6361 acacgacgct gagccgggag gccgtcaagg gcatgccgca cgccgaagaa gccggcggcc
     6421 tcagcggtgc gccggtgctg gccgccagca accgcgtcat cagccagctg cgcgcggcgc
     6481 tcggcaaaag ttttcccatc atcggcgtgg gcggcatcat gagcggccgg gacgccgtct
     6541 ccaagatcaa ggccggcgcc gacgtggtgc agatctacac cggactgatc tacaaggggc
     6601 ctgacctggt gaccgaggct gcgctggcga tcaagaaagg ccgctagggc ctgttcacac
     6661 ttgcataaac agtgtgaaca ggccccagcc caacagggat gggcgcaaca cgctcatgaa
     6721 ttgatagcgt ctagtccggc aaaccatcca ggatttttgc cgcttcgagc ttgcccattt
     6781 tccggagcct cgcaatcgcc ggcagctggc tggcgcgggg acgggtcttg ccgtcttccc
     6841 acttgtagac ggactgcacc gacacgttga gaagttttgc ggtcgcggca gcggtcagcc
     6901 cgagccggcg ccgctgggcg gccagtccct tggcgctgaa gcgcaatccg ctgccggcgc
     6961 cctcttcggc ttcctcgggt gcagccgcct tcctgccgga cttgagcaca cggctcagtt
     7021 gcttctccag ggccagcaca cgccttttca gggcggcgat atcgctgcgg tattgcgacg
     7081 aagctttttt cagcggctgg gtttccgcgc gggcgtcctt gcgggcaacc cgggatattt
     7141 cggctttgag aacagtagcg atattgggca cgtcgggaat ctccttgctg gatggacgat
     7201 gacttctcct ccgattgtag gaggcccgaa aaaccagccg tattgtgggc tatcccccgg
     7261 accaggtggg gacaggcgcc cgggcaccgc agcggccgct tgccagcctc agccggcgtg
     7321 cggggcgacg ggctggcggc tgcatgatcg ctatatattc gatagctgcc tgcgaccgtc
     7381 caccatgggc ttaagagcaa ttcgatcatg aaggacgata ccaccacagc caacgcgcaa
     7441 ggctggcggc ttaccttcag caggcgctgc ggcgcgtaga tagctgttcc atccaccgtc
     7501 cccggcgtgc gtctgcgccc gactcaatcg ggcacaaact gttgcttgaa ccgcaactcg
     7561 tcctcgggca attccagcgt gaccaactgg cccatcttgt tgtcggacgt ccggcaggcc
     7621 gcgaacaggc tggccttgcc catgaagttg tagctgtcca tgtcgatcag catgtagggg
     7681 taaggcacaa acacctgatg caggaagatg ctgcggtgcc ggttgcgcgg cgacggaaaa
     7741 agcttgtagt cgtcggtggt gatgcttttt gcggtggcca tgcggtgtgt ttcagtagtg
     7801 cggtggcagc tcgtcgccgg cgcgggtcag ggtggtggcg ccggcctcgg gcagttgctg
     7861 gcgcagctgg ctgacttcgc gcatcagcag gtcgatctgc tgctgctggc ggacgatgac
     7921 ctggttgagt tcgtcgagca ggtcttcgcc gaagctggcc ttgatctcca gatcggtcag
     7981 tcgtttttca atctcggtgg cggtttgcat accccctatt ggacacgagt tcggggcccg
     8041 aatagcagcc gatttcctac acgcagatac gccggcgtcc tctgctgcct gaggggtacc
     8101 cgggccatca tgaacgcttc acttcatcga ctggaaccat catgagcatt ctcggcaaga
     8161 tcttcagcaa aattttcccg tcggcacagg ctgcccagcc gccagcctcc tctggtccct
     8221 ccagcgatga cgtcgcggca gccgcagcgg ccgccggagc ggccagagca ggcgccgcca
     8281 ccgccgcacc ggccccgctg gcgcaggtcg atgtggaggc actgctcaac gacatggcgc
     8341 agaaaaaccc gcaaaaactc aactggaaaa cctccattgt tgacctgatg aaactgctgg
     8401 acctcgacag cagcctgggc gagcgcaagg aactggccaa agaactgggc tacaccggcg
     8461 acagcagcga cagcgccgcc atgaacatct ggctgcaccg ccaggtcatg aacaagctgg
     8521 ccgccaacgg cggaaccgtg ccggccgacc tgcgggactg aacgggcgtt tcgcgtcagc
     8581 cgtcccttca actttttttt ctggaacaca cactcatggg cattctctgg accattctta
     8641 tcggcttcat cgttggcctg gtcgccaaat tcctgatgcc cgggcgtgac ccgtccggct
     8701 tcatcatcac ggtgctgata ggtatcgtcg gttcggtgct tgccagctac ctgggcgggg
     8761 cgctgggcct gtaccaggtc ggcgagtcgg ccggtttcat cgcggccgtg ctgggcgcca
     8821 tgctgctgct gttcatctac cgcatggtga gcggcaaacg cggctgatcg ttacaagcaa
     8881 aaaatggctg cagcgcccgt gtaccgggcg tgtgcagcta tttttttggg agcaattcgc
     8941 cgacctggcg gccgatcgcc agcgagctgg tcaggcccgg cgactcgatg ccgaacaggt
     9001 tgaccaggcc cgccacgccg tgctccgccg gtccctggat caggaagtcc ctggccggct
     9061 cgtcgggcgc ctggatcttg ggccgtatgc cggcataacc agcgagtagc gcgccatcgg
     9121 gcaggcccgg ccagtacttg cgcacctcgg cgtaaaacgc gtcgccgcgg cgcgggtcca
     9181 ccaccaggtc ttcgggcgag tccacccact gcacgtcggg gccgaacttg gcctgaccgc
     9241 cgaggtccag ggtcaggtgc acaccgagcc cggccgcttc cggcaccggg taaatcaggc
     9301 ggctgaacgg cgaacggccc gacagcgtga agtagttgcc cttggcgtaa tggctgggcg
     9361 gcacatgctg cgcgtccagc cccgcaaagc ggctggccag cgcctgcgcg tgcagaccgg
     9421 cggcattgac cacgctttgc gccgccaatt cggtgccgtc ttctgctacc agaacaatag
     9481 ctgatggcgc acagtgcgcc tgggccagcg gcgaattgag caccaccatg ccgccggcgt
     9541 tctcgaggtc gccctgcagc gccagcatca gcgcgtggct gtccacaatg ccggtgctgg
     9601 gcgacaacac cgccgccacg cagtccagcg caggttcgag cgcctgcgcc tgctcgcgcg
     9661 tgagcagcac caggtcaggc acgccattgg cggcggcctt ggcgatgatg gcctgcagtt
     9721 ggggaatctg cgacgcatgg gtggcgacga tcagcttgcc gcagcggctg tggccgatgc
     9781 cgcgctgcgc gcagtagtcg tacagcagcg ctttgccctc cacacacagc cgcgccttga
     9841 gcgagccctg cgggtaataa atgccggcgt ggatcacctc gctgttgcgc gagctggtgc
     9901 cggtgccgat ggcgtccgcc gcttccagca ccatcacctc gcgcccctgc agcgccagcg
     9961 cacgggccac cgccagcccg accacacccg cgcctatcac caccacatcc acgttgtcca
    10021 tgaacctaga aaccgcagtt gaacgcgccc gagtgggccg tcatggggtg ggctggcaag
    10081 gcgcgacaac gctgcgggca gatgcccgcg aggaggagca acgccgccag cgcgccccag
    10141 ggcggctcaa tcgggtgcgt agcgctgtca ccccaatggc ggcggttttc gggtctgaaa
    10201 aagtaagtgt tcatgcggtg tagccagcga caagcaagcg atcaactgtg gattctaggt
    10261 tcaatgctcc tgttcaaaac cgtcgatgac ctggaccgca ttgctgccca gcgcctcggc
    10321 cgcataaccg ccttcgagca caaacaccgt cggcaggccg agcgccgcca ggcgccggcc
    10381 gagccggccg aagtcggccg cctgcagcgc aaaggtggaa atcgggtcat ccgcgaaggt
    10441 gtccagcccc agcgacacca ccagcgcctg cgcgccgtgc cgagtgatgc gccggcacgc
    10501 ctgctccagc gcctcgaacc aggcgctggc cggcgcgccc
//
LOCUS       DAIDFG010000081        10534 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_81_length_10534_cov_17.529249, whole genome shotgun sequence.
ACCESSION   DAIDFG010000081 DAIDFG010000000
VERSION     DAIDFG010000081.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 10534)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10534)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10534
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81_length_10534_cov_17.529249"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1990 a         3379 c         3309 g         1856 t
ORIGIN      
        1 ttcaagcagg ggccgcgggt ccggctttgc cggcccgcag gcgcagcggc ccccttgggg
       61 ggcagaaagc tacacgcagt gagcgaacgt gggggccacg tcctaacgtg cctcgaactc
      121 cggttcggac cattgcgaag gtgactcggc gccgggcagc ccgaagtcca tcggcgcgct
      181 gggcagggaa tccggtcccc gttgttgccc ggccgtgatg gcttcatgca tgcggtgcgc
      241 cacataccag ctggagcgaa ggcgcgcttc ccgggtatgg gaccccaggc ggggcgtgat
      301 gaacaggttc ctcaatccct ggacgggtga gccggcagcc gtgaagttgc tctcggcgcc
      361 gtccagaatg caggcttcga tgcggccatc acacaaggcg gcagccaatg cgttttcatc
      421 aaacagtgcg ctgcggctga tacccaccca caactggccg tgcttgcagg cggccagcag
      481 cttctcgttg acaaagcttc ggaagcgctc ggcataaagc atctggaccg acaccgcatc
      541 cgcctggctg accagttcgg tcagggtcac cggctggatg cgcagacgct cccagatcgg
      601 cgcggtatgg tgaaccgcag ggtcataacc gatcaggcgc acgcccaggc ccgtgagcat
      661 gccggccagc gtgtgcgcgg tcggggccag cccgaggatg cccaccgtgc tgccgtgcaa
      721 ctcgcgaccc atctgggtgc tgggatggcg gcgccccatc agggccgcca ccacaccccg
      781 acggtacagc agcagcaggc tgctcagcag gtactcggca ttggagcgca cattggcgct
      841 gctggcgtgg atgaccttga taccgcgctc cttgcaggcc tccaggtcgg tgttgtcggt
      901 gcccacatgc aggcggccga cggccttgag cttgggcatg aagtcaagaa actcgcgggt
      961 caccaccgtt tgacgcggga acacgatgcc gcggcttttg taagccgcct tgcgcaacac
     1021 caccatgtcg tccgcgagtt cgggacggta ttgcacgtta tgccgggttt cgagccaggc
     1081 catggcttct ggaaccaatg catcaagaag taagatgtcc acgtcgtgtc ctgtttgctg
     1141 ttattctttt tcgtcatgct tctgactctg tgtcagtaac gcaacactct agttccattc
     1201 ccaagccaag taaatgaaaa aagtcacaac tttaaagaag gcggttttcc ccgtcgcagg
     1261 cctcggaaca cggtttttgc ccgctaccaa agctcagccc aaggaaatgc tgcccgttgt
     1321 ggacaagccg ctgatccagt acgcggtcga agaagcctac gcggcgggca tacgccacat
     1381 gatttttgtt acaggacgca ataaacgggc catcgaagac cacttcgata ctgcctatga
     1441 actagagacc gaacttgaag ccgccaagaa gcaccatctg ctggaaatcg tccgctcgat
     1501 gaaccctggc gacatggatt tttcctatgt acgccagccg cgctcgctgg gtttgggcca
     1561 tgcggtgcta tgcgcagaac acctggtcgg cgatgaacct tttgcagtgt tgctggccga
     1621 tgatctgatg gtgggcgagc ccccggtcct ggcgcagatg gccgcccgat tcgagcaagt
     1681 ccagcaatcg attctggcgg tgcaggaagt gccgctggag cacacccggc gctacggcat
     1741 cgtggccacc gagcctgcgg tcgacggcct gatgaaagtc aacaagatgg tggaaaagcc
     1801 agcgccggaa gtggcgccct cacgcatggg ggtggcagga cgctacatcc tgacgcctgc
     1861 cgtgttccgg cacatacgca gccagcctac cggcgttggt ggagagattc agctgaccga
     1921 tggcattgcc ggcctgatgg gtgaagagag cgtctacgct tttcagtacg ccggcaaacg
     1981 gtatgactgc ggcagcaagg aaggtttcct cgaggccacc gtcgagctgg ccctgcagca
     2041 ccctgaagtg ggcgcccatt tcagaaacta cctggaaaac ctgcagatcg gctggtcggg
     2101 ccaggaacgc agaaaaccgc attgagcgct tgactgcgat tcactgatat tcagcggcgc
     2161 ttgagcacgt gggtgaagtc gccgccttcg gcctgctgct ccaccagctc gttgccggtc
     2221 tgcttggcaa acgccatgaa gtcgcgcgtc gagccggggt cggtcgaaac cacccgtagc
     2281 aactggccgc tgctcatctc ggccagggcc tttttggcct tcaggatggg cagggggcaa
     2341 ttcaggccgc gggtgtctaa ctctttgtcg atgtgcatgc gtttgtcctt ggatgcactg
     2401 attttaagca attcctgcgc tggcacgggc ttgtcacgcc gtatacccac ggccggcccg
     2461 cgcattgccc aattgctact ataaagatag ctaacaacgc ttgatcagca agggctcaag
     2521 gccaattcct tgtaaatccc aaaaacaaac ccccggtcct gcagggcagg ccgggggtct
     2581 ggcgccggaa aaaaaccctt cgtctggctt atttcatgcc gaattttgcc ctggcatcgt
     2641 tctggcaggc atccttggca ttgccggtca tggcatcgca acgctccttc gcagccttgt
     2701 actgggcatc atttttgtca gcagatgcgt cttttttggc atctgccatg gcgccggcct
     2761 tgtccttgtt ggcctcggaa ctgaccttgc tgaccttggc atcggctctg gctgtcgtgc
     2821 gggcggcttt ggcgtctttc acacagacat cctttgcatt gccgttcaag tcatcgcatt
     2881 tttccttggc gatgtcgtaa gcggcatcgg ccttggcctc tgccacctgt tgctcctggc
     2941 gagggctggg tttgtactgg gcttcaagtt cggctttggc gactttttcc gcgcccttgg
     3001 cttcggcctg gcagatatcc ctggcgttgg ccgtcaggga cttgcatttg tcccggctgg
     3061 ccttgtagtc agactcaacg cggtctttct gcgctttgta ttcgtccttg gtcagtgcca
     3121 tggcgtttcc ggcgaaggca caggacacgg cgagcgtgag catgatcagt ttcgatttca
     3181 tgggtgattt ccttttcgat gaagtgatgt atttttccaa gcgggtccgg ggctgaagat
     3241 cggccggcac gccataacgt caataacgct caaaaaaacc ggttgggttg cgttcctgaa
     3301 aggccgccag ctgtttttca gcctcgtcct cggcaatgcc gtgccgctcc tggatgcggc
     3361 ccagcaactg gtcgcgcttg ccggcaatga tgtcgaggtc gtcatcggtg agcttgcccc
     3421 actgctcctt gaccttgccc ttgaactgcg tccagttccc ctcgatgcga tcctggttca
     3481 tggtgactcc tttggtgaat gtgtgtcacg cggctgctag gccatgtgac agggaaattc
     3541 actgtaaaag tactgcctgc agcgtcaggt aggcacacgc gggcgatggg cgtaggtgcc
     3601 tgcctacacc ccaagctgaa cgggacagtg cctgaagcgc aggcggccta acgcgcgcgc
     3661 agcgagcggc ccagcagcag gaccggaaac agaccgacca gtaccaggac cagcgccggc
     3721 agcgctgcct cgcccaggcg ttcgtcgcgg gccagctggt aggtcaccac ggccagcgtg
     3781 tcgctgttga agggacgcag caccagcgtg gccggcaatt ccttcatcac atcgacaaac
     3841 accagcaggg cagccgcccc gaccgaccgt ttgagcagtg gccagtgcac tctcgccatc
     3901 agtgctgcac cggtggtacc cagcatgcgc gcactgtcgt cgaaactggc cggtatgcgc
     3961 gagtagccgc tttgcacgga ctgcaacgcc acggcggtga aacgcaccag gtaagcccag
     4021 acaatgccca gcgccgtccc cgtgatccag taccccgcgg aggtttgcgg ggccacggcc
     4081 tgcagccagc ccacgggcag cagcaggccg accacaatca cggcgccggg cacggcataa
     4141 cccaggcttg ccagttgcgc agcgaagcgt gtcagccagt ggggctggcg gcgcagcgca
     4201 aagctcaggc tgagtgccac accggtggcc agcacagcgc tcaagccggc caggctcacg
     4261 ctgttgaggg accagcgcac aaactgcgtc cacggcaact cgctccagcc ggccaccagg
     4321 gggcgcagca tgaacagcac gggcagcaca aacccgagtg tgatgggcag tgcacacaag
     4381 gcccacgcca aaccggcccg caggccgtgc aagggcgtgg gcgcagcgtc ggcagccccg
     4441 gcgcggccac cgcggctgcc ggcaaaacgc atgcgttttt gcgcatggtg ttcgattctc
     4501 agcagcacag ccaccaccag caacaagacc gtggccagct gcgctgccgc cagccggttg
     4561 tccatggcca gccaggcctt gtagatgccg gcggtgaagg tctggatccc gaaatagctg
     4621 gacacgccga agtcggccag cgtctccatc aaggccagcg ccacacccgc cgccacggca
     4681 ggacgggcca gcggcagggc cacctcccga atgcggcgct ccagcggtgc gccgagcaag
     4741 cgggcggctt ccatcaaatg cacggcccgc tcggacagcg cggtgcgggc cagcagatag
     4801 acgtaggggt acagcgtgac cgtgaaaacc agcaccgccc cgcccaggct gcgaatttca
     4861 ggcagcagac gcccctgcca gccaaagctc gcgcgcagcc agctttgcag gggcccggcg
     4921 tattgcagga agtcggtgta cgcataggcc accacgtagg ccggcatggc cagtggcagg
     4981 agcagcgccc aggcaaaaaa ccgccgcccc ggaaactcga acagcgtcac cgcacaggcc
     5041 gtgcccatgc cgatcagcgc aacgcccccc gctacggaca aacacagcag cagcgacgtg
     5101 aaggcgtatt ccggcaggac ggttcgcagc atctcccgca ggatgggcag ggactggctg
     5161 tcgagttcaa accacgacag catcaccgcc agcaccggca gcgtgagcac caccatcagc
     5221 aggaacaggg gcacggatga aaaaaagcga cgaagcatga agtcccgaaa aaaagcctct
     5281 gctcacgccg catggcggat acctgatgca gacggcgggc cgcatatcag gcgagtgcaa
     5341 atgagaattg taagcatcta caattgcacc catgttcctg gaactttccc acctggtggt
     5401 gcattacgcg ggccgctcca gcccggccgt cggcggcgtg tccctgggac tgcgcaccgg
     5461 agagattggt gtgctgatcg ggccctccgg ctgcggaaaa accaccttgc tgcgggccgt
     5521 cgccggcctg gagcaggccc aagccggcag catctcgctg gcacaggaaa tcgtcagcag
     5581 cggcaagcaa catgtgcctg ccgaacagcg gcgtatcggc atggtgtttc aggactacgc
     5641 cctgttcccg cacctcgacg tgggccacaa cgtggccttc gggttgacgc acctcaaacc
     5701 ggcggagcgc cagcagcgtg tcagcgaagt gctggacatg gtcggtctgg gcaaggccca
     5761 gaaacgctac ccgcatgaac tgtcaggcgg gcagcagcaa cgtgtggcgc tggcgcgcgc
     5821 gctggccccc cggccgcgcc tgctgttgct ggacgagcct ttttccaacc tcgatgtgga
     5881 tttgcgcgaa cgcctggcgc atgaggtgcg cgccatcctg aagctggccg gcgccacggc
     5941 gctgtttgtc acccacgacc agctcgaagc ctttgccatc ggcgaccgta tcggcgtcat
     6001 gcatgagggc cagctacacc agtgggatga ggcttacgag ctgtaccacc ggcccgcgac
     6061 ccgttttgtg gccgaattca tcggccacgg cgtgttcacc cacgcccagc tgacacagcg
     6121 ggaccacaac gtggtggtcc acacggcact gggagacctc agcggtttgc tggagtgccc
     6181 cctgcccagc gcctaccccg acggcatctg cgacgtgttg ctgcgcgccg acgacatcgt
     6241 gcatgacgat tcagcgccgg tgaaagcgca aatcctgcgc aaggcttttc gcggctcgga
     6301 atttctctac accctgcagt tgcaaaccgg cgaaaccgtg atggcgcatg tgccttcaca
     6361 ccacgaccat gcactcggtg aatggattgg cattcgcgcc gaagtcgacc acgtcgtgac
     6421 gttccagcgc gacgaacacg tgtccgctgc cgcctcagat taagaatcct cgcgcagcag
     6481 gcgctccact tcgcgctgca gatcggcagc ccaggtacgc cggtcccggc catcgcaggg
     6541 ctggagctcc ccgaaggtga cctcggcgcg aagatgccgc acgttcaggg tttcccagat
     6601 cgacacgaaa atcgaatcgt cgtcgatgta acgcggcgca aaacaggtct gccccgtatc
     6661 ggcgtccaca aagcgcaggg ccaccggttg cacaggtgcg tccgccgcaa tcgccgcctg
     6721 cagcaggttg gcatgaaagg gcttgaggcg ggtgctgtcg cccgtcgttc cctcggggaa
     6781 cacggcgagc acgtccccga gcttgagctg ccgggtcatg tggtgcacca cgcggtgggc
     6841 atcccgcctg gactcccgct ctatgtacag ggtgccggca ccgccggcca gcgtaccgac
     6901 cagcggccag tgcttgatat ccgacttgga aacaaaacgg cagtaacgcg aggcatgcag
     6961 caccacgatg tccagccagg aaaggtggtt ggccacaagg agggccggcc cctctgccac
     7021 gggtgacccc ctgaccacca gctcgatgcc gacgatcttc aacatggcca tggcccagac
     7081 ctggaccctg gcttctttcg ccggctgccc gagccgggga aacagcgtca ggatggtcca
     7141 catgccgacc gcgatatgcg ccagggcgcg caacaggcgc cagacggcct gcatgccgtt
     7201 caggcctcgt atgcgacttg cccgcccacc agcgtccagc acacgcgagc cggcagctgg
     7261 tagccggaaa acggcgtgtg tttgccctgg ctgcgcaagg tgtcaggctg caccgtccat
     7321 tcggtctgcg cgttgaacac acagacatcg gccacgccac ccttgaccag ttgcccggca
     7381 ctggcctgaa aggtccccag tgcgccgccc aggacgcggg ccggctcact ggtaagcacc
     7441 gccagcgcgc gggacaggcc caccccgctg tccgtacccc atttgcaggc caggcccagc
     7501 aacagttcca gcccggtggc acccggttca gcctcggcaa aaggcagagt cttcgcatcg
     7561 gcctccaccg gtgtgtggtc ggacaccagc gcgtcgatga cgccgtcggc cagcgcctga
     7621 cgcaaggcat cgcggtcacg ctgctggcgc agcggcgggt tcaggcgcat gcggctgtcg
     7681 aagtagccaa tatcggtgtc cgtcagatgc aggctgttga tgctgacgtc cgccgtcaca
     7741 gccaggccat cggccttggc cttggccagc aaggccacgc cggccgcgct gctgatgcgg
     7801 cagacatgca ctcgcgcgcc agtggcacgc atcagctcga acagcgtgtg cagcgcgatg
     7861 gtctcggccg ccaccggcac accgctcagg cccagccggg tcgccagcgc accgctcgcg
     7921 gccacgcccc cgcccagcca gctgtcctgc ggacgcagcc agacggtgta gccaaagctg
     7981 gcagcatatt gcagggcgcg cagcaacacc tgggtgttgg ccagcggcac ctcggcctgg
     8041 ctgaagccga cacagccggc ctccgtcagt tcgaccattt ccgtcaatgc ttccccctgc
     8101 agggcccgcg tcaacgcgcc cagcggaaac acgcgcgact ggtgcaattt ttctgcacgg
     8161 aacttgagca tctcgaccag gccgggctca tcgagcaccg ggtcggtgtc gggcggacag
     8221 accaggctgg tgacgccgcc ggcgacggct gccgccagtt cgctttcgag catgccctca
     8281 tgttcgtagc ccggttcgcg caggcgcgcc gacaggtcga tcaggcccgg cgcaacaatg
     8341 cagcccgtgg catcaatcgt gcggttcggc gtgaaatctg caaccgtggt tttcagcgac
     8401 agaatgcgcc cggcggcaat cgccacatcg cccacttcgt cgaggccgga agccggatca
     8461 atcacccgac cgttcttgat cagtatcttc attgtgtagc ttccagaaaa ggcgcaacgc
     8521 gacacatgca gaggcatgca tggaggctct tcaatccaag caggggccgc ggaaccggct
     8581 tcgccgggcc gcaggcgcag cggcctcctc ggggggcagc gcagtacgcg aagcgacaag
     8641 cgtgggggct catgttcatg cttcgttccc ggcaacaatg ctcatcaccg ccatgcgcac
     8701 ggcgatgccg aaagtgacct gcggcaggat cacgctttgt gcgccatcaa ccacggcgga
     8761 atcgatttcg acgccgcggt tgatcgggcc gggatgcatg acgatggcat cgggtttggc
     8821 atgcttgagc ttctcgttgg tcaggccgaa gctcttgaaa aactcatggc tgctgggcaa
     8881 cagggcgccg gacatgcgtt cgttctgcag cctgagcatg atgatcacgt cggccccctt
     8941 gatgccctct tccagggtgt ggcagacgcg cacccccatt tgcgccatgt cggcaggcac
     9001 cagggttttc ggacctacca ctcgtacctc ggggcaaccc agcgtggtca gcgcatggat
     9061 gtcggagcgg gccacacgcg agtgcagcac atcgccgacg atcgccacgg tgaggttgct
     9121 gaagtccttc ttgtagtggc ggatggtgta catgtccagc agcccctgcg tcgggtgtgc
     9181 atggcggccg tcgccggcgt tgatcacatg cacgtgtggt gcgacgtgtt gcgcgatcag
     9241 gtaaggcgca cccgattcgc tgtggcgcac cacgaacagg tctgccgcca tcgcgctcag
     9301 gttggagatc gtatcgagca gcgactcgcc tttggaggcc gacgagcgcg caatatcaag
     9361 gttgatcaca tcggccgaca gccgcgtcgc cgcgatttcg aaggtcgtgc gggtgcgggt
     9421 ggagttttca aagaagaggt tgaagacact cttgccgcgc agcagcggga cctttttgac
     9481 ctcgcggtcg ctgacgctga caaagctggc cgcggtatcg aggatgtggg tcagcgtcgc
     9541 tttgggcaga ccttcaatgg acagcaggtg aatcagctca ccattcttgt tgagttgcgg
     9601 gtttcgtcgg tacaacacaa tcaggccttt cgcgggacag caggaatact gacgggaaaa
     9661 ccgtcggggc aggtggtcat ggtgtcaccc cctgcacctg gaagctgaac cggccgtcgt
     9721 cactgcgcgc cagtgccagc gactgggacg ccggcagggc cacccgggca gccgcaaagt
     9781 cggcctgtat cggcagttcc ctgtcaccac ggtccaccag caccacaagc ttgacgctgg
     9841 ctggccggcc gtaatcaaac acctcgttga gaatggcacg aatggtgcgc ccggtgtaga
     9901 gcacgtcgtc gagcacgatg aggtgggcgc cgttgacatc aaaaggcagc accgtctggc
     9961 cgctggccga caggccgcgc tgggaaaaat cgtcacggtg catcgccgag gacaaggtac
    10021 cgatgtcgtc agctaggccc aggtcggcgc gcaggcgttc ggccagccag acgccgccgg
    10081 aagccacacc taccagccgc ggcgggcggt cataggtcgc cagcagctgc tgcatgccgc
    10141 gcaggacttc gcggtacaag gcctcggcgt ccagggtcag gctggtcatg aaaaactcct
    10201 taaaaactgc tcgaggatta tgcaggcaga cgctgcgtcc gcatcaggcg caccctgcga
    10261 cagcgcttcg gtggtgctgt agcgctcgtc aacctcaaac acctgcagac cgaaacgccc
    10321 gcgcaactgg cgggcgaatt tccgggcgcg tgcggtgttg tcgtggctgg cgccgtcggg
    10381 atggaaagga atccccacca ccagggcgtc gggttgccac tcccggattt tcagggcgat
    10441 cgccgcgaaa cgggcgtcac cctcggcggc gatgctgcct tgcggtgtcg ccgtacgcgt
    10501 cagccggttg ccggtggcga caccggtgcg cttg
//
LOCUS       DAIDFG010000082        10468 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_82_length_10468_cov_17.827811, whole genome shotgun sequence.
ACCESSION   DAIDFG010000082 DAIDFG010000000
VERSION     DAIDFG010000082.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 10468)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10468)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10468
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82_length_10468_cov_17.827811"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1937 a         3181 c         3248 g         2102 t
ORIGIN      
        1 ggcgtcaagg aactgctggc gcaaaacggg attgcgtgtg ctcagggcct gcgaggcgta
       61 gagcgcctgc gcgcagacct cgccgacatg gttgacccgc atcagtgcgc cggccagcgc
      121 cttgtccgcg acgctcagtt cggtgtcctg tgcgggcacg gtcgggcatg tccggctggc
      181 acggggtttg gcaaacaggg tgcgcagggc gctgtcggcg gaagtgagca gggcatccat
      241 gatggtattt atcgcttgtc agtgggcgga agtgcagatg aaagtttatc cagtcggacc
      301 tcaagctggc ggacacgttc cccgagggat gcgtccggtg cggctatttc ggccggcaac
      361 cggtcttgta tgccagacaa cctcccctga ggcgcaggcg tgggcgaggg ggtcagccgc
      421 gccacttcct gtccacgccg ggtgatgcag aaggcctcgc cacgttcaac cgcggcaatg
      481 agcctcgaaa aattctggtt ggcgtcgcgc agcgaaatca gctggaatgt cattgggaat
      541 ggtgggccgg aaagttatcg aacgcggcta tctttttgtc cagatgattt ttgtaaatgt
      601 agtacaaatt gtagtacatg agtatcgacg ccagccagct tttgaaagac gaataaatgc
      661 cgcgcaagcg gtttttgcgt ttgttgcagg ggtgcaacga aattgaattt atcgcgccaa
      721 ttcctttgcg aaagctctca gcctctggtg caatacatac aacttcctaa atggaggttg
      781 gcccggggat ccaaaatcct ggagccctat gtttaacctt ggagaaactc gcaatgaaaa
      841 aatccctagt cgctttggct atcctggctg ctgccggcgt ggcttcggcc cagtcttccg
      901 tgaccctgtt cggcgttgtt gacgtgaact acgctcacgg ctccggttcc ggcgttggtt
      961 cttccagcaa gagccaactg acgagcggtg gttacaacac cagccgtctg ggcttccgtg
     1021 gtacggaaga cctcggcggt ggcatgtctg ccagcttctg gctggaagcc ggcataaacg
     1081 ccgacaatgg cacctttcaa gccacgaaca ccaacaacca ggctaatggc gcttccgtgg
     1141 gcagcggcct gaccttcaac cgtcgttcga cggtcagcct gtccagcggc ctgggtgaac
     1201 tgcgtctggg ccgtgactac acgccacagt tctggaatca gaccgtgttt gatccgttcg
     1261 gtacggttgg cgtgggcggc tcccaggcca acgtttccag ccttggtggg ccagtcagcg
     1321 ttcgcgcttc caacagcgtt ggctacttcc tgccaggcaa tctgggcggc ttctacggcc
     1381 agttccagca ctacctcggt gagaacaaca aaaccggcgt ggcgactgaa aaagacggca
     1441 ccggttctgc catccgcctc ggcttcgcca acggcccgat caacgtggct ctggcaacgt
     1501 ccagcaccaa gtacgctgcg accgccacca cgggcaagat ccagtccacc aacattggcg
     1561 gttcgtatga cttcggtgtc gcccaagcca tggctttggt gacccgtgac aaagttgact
     1621 ctgtccccac caatgtgacg ggcaagggcg ctttgatcgg tgctttggtc ccaatgggtg
     1681 ccggccagat ccgtgtggcc tactccacct acaaaaccga cgctgcgacc acgcctaagt
     1741 ccaaaaaatg ggctctgggt tatgtgcaca acctgtccaa gcgtacggct ctgtacacca
     1801 cgatcgctcg cgtgaccaac agcggtggtg cttcccaggc tctgaacggc gccgtgactg
     1861 cagcgaacgc acgttcgacc ggttacgact tcggtatcaa gcactccttc taattgatgg
     1921 ctggtcttgt gccagcttca atcaagagag tttaaaaagc cgccttcggg cggctttttt
     1981 catggaaaat gtaaaagcgt gccactttgg gggagatgct gatgacgcag gttgaccggg
     2041 cgcaacggtg gggtaggtcg ggtggtggcg tgatgttctt ggccttgctg cagcttgtgg
     2101 gctgtgcatc tttcatgccg ccggctaccc cggctgatcc cgcacggctc agcgccaccc
     2161 cttgggtgac tgcgtccacc ggtgaccggg tcacgccagg ttggcggcac tacccttttc
     2221 cgggcaagaa accgagccac tttcgttatg agcgcaagga cgggcgcgag gcgatggctg
     2281 caacttccag ctcttctgcg agcatggtgc gccaggcgct gcgcgtggag ccagatgatc
     2341 tggccagcgt gcgcttttcc tggaaagtgc ctgagctgat tgcgcaagcc gacatggcgc
     2401 tgcgcgacgc tgatgattca ccggtacgcg tggtgctggt gtttgaaggc gaccgcagca
     2461 agttctccag caagaatgcc atgttgtccg aactggcacg cgcgttgacc ggcgaggagt
     2521 tgccttatgc aacgttgatg tacgtgtggt gcaacacccg cccgccgggc acggtgattg
     2581 tcaatccgcg taccgacagg atacgcaagc ttgtcgtcga atccggcgcc ggcaagctgc
     2641 gccagtgggt cgactacgag cgcgatattc gcgcggacta tgaaatgact ttcggcgaag
     2701 cgccgggcgc gctggtgggc ataggcatca tgaccgacag tgacaacacg cgttcgaata
     2761 cctccgcctg gtacgggccg gtcacgctcg acaaggttgc gcacgcgcac tagctgattg
     2821 cctggcctga gagttgcaag cgtctgacgg gcgtgcaccc cgggcgcatc gccttggccc
     2881 tcgcgcaggt ttcagggggt cccgttcagg ggaaatccct tgctttgtcc tgaggagcag
     2941 gttgtatatc tggtgcttac tttccagggg aaaaccatgc aacttcaccc gcttttttta
     3001 cagcctgcag tcgctggtcc gcagcgtggg cgccggcggt gatggcaact acaacgttgg
     3061 ccgctacagc aacccgcgca tggactacat cgtggaccgt gtgaaggtgg agaccgattt
     3121 gccggtgcgt aaccgcctgc tgaccgaggc cttgcagctg tcgaacgaca cggtttcgca
     3181 tattccgctg cacaaccaga tcattccgtg ggcgatgaag aagaacattg atgtcgtgca
     3241 ccgcgccgac aaccggctcg actggcgact gatcaaggtc aactgagcgt gtaatccaag
     3301 agggcttttc ggggcccttt tttgtagttc ttttctttag gcctaaagga aatcccggtg
     3361 tcggcacagt gcttgctctg ctagcatggg ctctcgctca aaaccatacc cccgaatgtt
     3421 tgcatttatt ctccgtcgcc tgctccaggc tgtgatcgtg atggtcgctg tggcttttat
     3481 tgcattcatg ctgttccagt atgttggcga cccggtggtg tttctgcttg ggcaggatgc
     3541 gcagcctgac cagattgcgc agctgcgcgc cgatctgggg ctggacaagc cctttttcgt
     3601 gcagttcggc cactttctgg tcaatgccgt gcagggcgag tttggtctca gcctgcgtca
     3661 gggggccaag gtctcacgcc tgattgcgga gcgttttccg gccacgctgg agctggctct
     3721 ggtggccgcc gtcatggccc tggtgattgg tgtaccgatg ggtgtttatg cggccctgcg
     3781 ccgcggaact tttctgagcc agctgctcat gacggtgtcg ctggtgggcg tgtcgcttcc
     3841 gaccttcctg atcgggatcc tgctgatcct ggctttctcc gtaggcctcg gctggtttcc
     3901 cagtttcggg cgcggtaacg tggtgcagat gggctggtgg agcagcggcc tgttgactgc
     3961 caagggctgg caccatatcg tgcttccggc cgtgacgctg gcgatcttcc agcttacgct
     4021 gatcatgcgg ctggtccgcg ccgaaatgct ggaggtgctg cgcaccgact acatcaagtt
     4081 tgcgcgcgca cgtggcctgt ccaaccgcgc catccatttt gggcacgcgc tcaaaaacac
     4141 cctggtgcct gtcatgacca tcaccggcct gcagctgggc ggactgatcg cttttgcgat
     4201 catcactgaa accgttttcc agtggccggg catgggcctg ctcttcatcc aggcggtgac
     4261 ttttgccgac atccccgtga tggccgccta cctgtgtctg attgcactga tttttgtcgt
     4321 gatcaacctg gtggttgacc tgctgtattt tgtggtggac ccacgtttgc gcgtgggttc
     4381 ggcggggggg cactgatgtt gtcgccccgg cctgctccgg tgctcaaggc caacggccgc
     4441 gccttcgcgc aaatcagcga gttccatccc gaaatcacgg cctttcgccg cgacctgcat
     4501 gcccatccgg agctcggctt cgaagaggtc tacaccagcg cccgcgtgaa ggaagcgctg
     4561 cagcgctgtg gcgtggacga ggtgcatacc ggcatcggca agaccgggct ggtggccgtc
     4621 atcaagggcc agcagagtga cagcggaaaa atgatcggcc tgcgtgccga catggacgcg
     4681 ttgccgatga cggaacacaa cgactttgcc tggaaatcct cgcggaccgg catgatgcac
     4741 ggttgcggcc atgacgggca tgttgccatg ctggtgggcg cagcccgtta cctggcggcc
     4801 acgcgcaatt ttgacgggac ggccgtgctg atttttcagc ccggcgaaga gggctttgcc
     4861 ggcgcgcggg tcatgattga agacggcctg tttgaccggt ttccggtgca ggcggtttac
     4921 ggcatgcaca actggccgtc catgaagccg ggcacggtgg gcatcaaccc ggggcccatg
     4981 atggcagccg ctgaccgcat caccatcgac atcaccggcc gcggcgggca tggcgcccac
     5041 gcctacatga cggtcgaccc ggtgctggtg gcggggcaca tcatcacggc ggtgcaaagc
     5101 attgtctcgc gcaacgtcaa gccgattgac agcgccgtga tcagcctgtg cgcaatgcac
     5161 gccggcgaca tgggcgcctt cagcgtggtg ccgggcaagg ccaccctggt gggcacggtc
     5221 cgcaccttca acccggacgt gcaggacctg atagagcgtc gtctcaacga actctgcacc
     5281 gcagtcgcgc tcggctttgg tgccaccgcc gccgtgcatt acgagcgcat ttatccggcc
     5341 accatcaacg cgttcgatga agccatgttt gccgccgatg tggcggaaac cttgctgggc
     5401 gaggaccacg tcgtgcgcgg catggagccc agcatgggcg ccgaagactt ttccttcatg
     5461 ctgcaggtca aacccggcgc gtacatgcgc atcggccagg gggccgaaaa cggtgtcggc
     5521 agctgttttc tgcacaacag ccgttacgat ttcaacgacg acatcctgcc gctgggtgcc
     5581 gccctgcatg ccgggctggt ggagcaatcc atgcctttgt ccgtgccgcg ctgattattt
     5641 ttttgttcaa caggagagtc ccatgaattt cacgaaaaaa gccgcttttg cccttgtggg
     5701 tacagcgctg gcagctacaa cttttgtagc gtccgcagtg accctgcggg tcggaaacca
     5761 gggtgatgcc ctctcgctgg atccgcattc gctcaacgaa tcgctgcaac tgacggtggt
     5821 gggcaatgtg tacgaaccgc tggtcacgcg cgatcgcagc tacaagctgg ccccctcgct
     5881 ggcaaccgac tggaagcaga cctcgccgac ggtctggcgc ttcaatctgc gcaagggcgt
     5941 gcagttccat gacggctcgc cgttcaccgc ggacgatgtg atcttcagct atgaacgcgc
     6001 caagggtgac ggctccgaca tgaagagtta tgtcggccag atcaaggaaa tcaagaagat
     6061 caacgatcac acgatcgact tcgtgctgaa caaccccttt ccgatcctgc ccgagctgtt
     6121 cacgcagtgg cacatcatga gcaagaagtg gtgcgaaacg aaccaggcca ccaagccagt
     6181 ggaccgccgc aaaggcattg aaaacgccgc gtctttccgt gccaacggca ccggcccctt
     6241 ccgcgtgcgt gagcgccagc ccaatgtccg caccaccttt atccgcaacg gcagctactg
     6301 gggcaagatc gacggcaatg tggacgaagt ggtcttcaac gtcatcggca acaacagcac
     6361 ccgcgtcgcg gcacttctgt ccggcgaaat tgacgtgatg gagccggtgc cggtgcagga
     6421 cgtggcccgc atcaaggcca atgccgagct caaggtgctg caaggccccg agctgcgcat
     6481 cattttcctc ggcatggacc agaagcgcga cgaactgcag ttttccagcg tcaagggcaa
     6541 aaacccgttc aaggacaagc gcgtccgcca ggctttctac caggccatcg acatcgaagc
     6601 catcaagaac cgtgtgatgc gcggtgcggc cacgccggtg gccacactgc tggcacccga
     6661 gatcaagggt tacgcccccg acctcgccaa acgcctgccg tttgacgtgg aagccgccaa
     6721 gaagctgatg gctgacgccg gttatggggc agggttcgag ttgaagatga actgcccgaa
     6781 cgaccgttat gtcaacgacg gtgaaatctg ccaggccgtg gccgcaaatc tgtcgcgcat
     6841 cggggtcaag atcaatctgg aagccgaaac caagggcacc tactttccga agatcttgcg
     6901 ccgtgacacc agcttctaca tgctgggctg gacgccgagc acggtggacg cccacaacgt
     6961 gctttacccc atcctatcca cgccaggtga gggtggccgc ggccagttca atctgggtgc
     7021 ctacagcaat gccaggattg accagctgac caacctggtg gcttccgaga ccgatgacaa
     7081 gaagcgcaac gagatgatcc acgaagcgct gaagatccat caggacgagg tgggccatct
     7141 gccgctgcac cagcaggcgc tcaactgggc tgccaaaaag aacattgaaa tggttcagtg
     7201 gcccgacaac gggatgccct ggaagtacgt caaagtcaac aagtaacaag ggttgatgaa
     7261 aagcgcgcta ttccgatggt gggatggcga cattggctac agctttcgca cctctccggt
     7321 ggcgatggct gcggctgcag tcgccctcat ctgcatcttc tgttcggtgt ttgcgggctt
     7381 tgtggcgccg cacaacccgt ttgacctgac gacgctcgag ctgagcgatg cccggctgcc
     7441 accggcctgg tcggccgagg gctcgagcaa gtacctgctg ggcaccgatg accaggggcg
     7501 ggacatcctc tcggccctga tgtacggtgc ccgcgtttcc ctggcggtgg gttttgcctc
     7561 ggtgatcctg tcggtcgtgt tcggcgtggg catgggtttg ctggccggtt tttatggcgg
     7621 ctggattgac tccgccctga tgcgcctgtg tgacgtgatg ctgtcttttc cggccattct
     7681 ggtggctctg ttgattgccg gggtggggcg ggccctgttt cccaatgcgc aggactccct
     7741 ggcgctgggg gtgctgatca tatcgatcac gcttaccgga tgggtgcagt acgccagaac
     7801 ggtgcgtggc tcgacgctgg tcgaacgcaa caaggagtac gtgcaggcag cacgtgtgac
     7861 cggtgtgaca ccgatgcgca tcatgcgccg ccacgtgttg ccgaatgtga tggggccggt
     7921 gctggtgctg gcgaccatcc aggtggccac ggccatcatc accgaagcca ccctgtcctt
     7981 tctgggcgta ggcgcgccgc ccacgtcgcc gtcgctgggc accctgatcc gcatcggcaa
     8041 cgactacctg ttttccggcg agtggtggat cacggtgttt cccggcctga tgctggtgtt
     8101 gatttcgctg agcgtgaacc tgctgggcga ctggctgcgc gatgccctga acccgcgcct
     8161 gcgctaaaca gccatcgcgc tgaaactgac tgcatttcaa catgagcctc ttacaagtca
     8221 agaacctgat cgtcgagttt cccggccggc gcggtacgct gcgggcgctc gacgacattt
     8281 cgtttgatat cgcgcccggt gaaatcctcg gcgtggtcgg cgagtcgggc gccggcaagt
     8341 cgctgaccgg cgctgccatc atcggcctgc tggagccgcc gggccgcatc ggcggcgggc
     8401 agatcctgct cgaaggcgag cgcatcgaca acctgcccta cgagcagatg cgccacattc
     8461 gcggccgcaa gatcggcgct attttccagg accccctgac ctcgttgaac ccgttgtaca
     8521 cggtgggccg ccagttgatc gagacgatac aggcgcacct gccggtcagc aacgccgaag
     8581 cgcgccagcg cgccatttcc ctgctgcagg acaccggcat tccggcggcc gagcagcgca
     8641 ttgaacattt tccgcaccag ttctcgggcg gcatgcgcca gcgcgtggtg atcgccctgg
     8701 cgctggccgc cgaacccaag ctgattgtgg ccgacgagcc gaccacggcg ctcgatgtgt
     8761 cgatccaggc gcagatcatc atgctgctca aaagcatctg caagaagcgt ggtgccgcgg
     8821 tgatgctgat cacccacgac atgggcgtga ttgccgaaac ctgcgaccgg gtggccgtga
     8881 tgtacgccgg ccggattgcc gaaattggcc cggtgcatga ggtgatcaat cacccgtccc
     8941 atccctatac cgcaggcctg atggaggcga tccccgacat cacgctggac cgcgaacgac
     9001 tcaaccagat tgacggcgcc atgccgcgtc tgaacgccat tccacagggc tgcgccttca
     9061 atccgcgctg ccccaaggtg tttgaccgct gcacgcagga caggccggac ctgctgcctg
     9121 ccggtgccac acgcgccgcc tgctggctgc acgatgccac ggtgggagcg accgcatgag
     9181 caacagtgaa acgacaagcg tgggcgaccg ttttcccgca gggccgcccc aagggaaaac
     9241 agcccccccg gggggcagcg acgtacgcgc agcgacaagc gtgggggctc ctctggttca
     9301 ggcttttgac ctggccaaga cctttgatgt atccccgccc tggctgaacc gcgtgctcga
     9361 aggcaagccc cggctgatgc tgcatgccgt cgacggcgtc agcttcgaga ttgaaaaagg
     9421 caagacactg gctctggttg gcgagtcggg ctgcggcaaa agcacggtgg cccgtttgct
     9481 ggtgggcctg tacgagccga cccgtggcgg attgaccttt gatggccagg atgcgcatgc
     9541 cgccttcaag acgccacagg gccggcaact gcgacgccgc atccagatga ttttccagga
     9601 cccgtacgcg agcctgaatc cgcgctggat cgtggaagac atcgtgggtg agccgctcaa
     9661 ggaacacggc ctgatcacca acgaggccga gctcaaggcg cgcgtgggcg aactgctcaa
     9721 gtccgtcggc ctgtcgccgc tggacatggt caagtttccg caccagtttt cgggcggcca
     9781 gcgccagcgc atctcgattg cccgggcact cgccaccgag ccggaattcc tggtctgcga
     9841 cgagccgacc tcggcgctcg acgtgagtgt gcaggcgcag gtgctcaaca tcatgaaaga
     9901 cctgcagcgc gagcgcgggt tgacctacct gttcatctcg cacaatctcg cggtggtgcg
     9961 ccatgtcagt gaccaggtgg gcgtgatgta cctgggacgg ttggtcgaac tgtccgacaa
    10021 gcagacgctg ttcgccagcc cgaagcaccc ctacacacgc atgctgctgg acgcgatccc
    10081 gaaaatgcat gacaccggcc gcgcccgcac cccggtgcag ggcgaggtgc ccaacccatt
    10141 gaacccgccg acaggctgca cctttcaccc gcgctgcccg cacgtcaacg accgctgtcg
    10201 cgccgagcgg ccgcagctcc tgaccatcca gggtgtgaag atcgcctgcc atgcggtgga
    10261 agaaggccgc atctaaccgc tccttttttt gtagctggtg gcgcttgttg tatatgggct
    10321 agaggctgat ttcattccca aatgggcgca aaaagggtgc aagcccttcg cggcatcacg
    10381 ctgcgcctgc ggtctgctcg ggacagtagg cgcaactccc cactcccacc cctaacccct
    10441 ccctcgcccc gctgggcgat ggaggggg
//
LOCUS       DAIDFG010000083        10413 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_83_length_10413_cov_18.875169, whole genome shotgun sequence.
ACCESSION   DAIDFG010000083 DAIDFG010000000
VERSION     DAIDFG010000083.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 10413)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10413)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10413
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83_length_10413_cov_18.875169"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2130 a         3232 c         3519 g         1532 t
ORIGIN      
        1 gacacacacg gtgatgcgcc gggcaagtcg atcagctacg agaaaggccg tgcggttgcg
       61 tcagatgaag gcgtgctgga agctgcattc acgggcaacc acggctggta ctggcgcaat
      121 cggggcaagt caaacgtcaa ggtcatcctg cgcacacgcg gtgactacac cgatatcaaa
      181 aaaatgatgt aaggcaatgg ggccgcggta gctaccgcgg ccccatttca ctgcacgagt
      241 ccacccggcg gtgatctcat ggcatgggcg actttacgga caggctaccg gtgctgccgt
      301 cacacaagaa aagtggctct agcccttgcc tactgtgcgc ggtaagctat caaaaacaga
      361 gcagttttga tgcccagtac ggtcatgagc agcgagccca gcacatgcag cgctgccgtg
      421 cccagcgcca ggccgtaacg ctcttccagc aggaaggtga caacttcagc ggaaaaggag
      481 gaaaaagtgg tgagcccgcc cagaaagccg gtcaccagca tcaggcgcca gaccgggtcg
      541 agctgcggca gggactgaaa aaaggccagg cagatgccga tcaggtagcc gccaatcagg
      601 ttggcggcca gcgtgcccca gggaatgagg ccgccgggcg tcagccacag cgccaggccc
      661 cagcgggcca gtgcgccggc gctggcaccc agacagatgg cgatgactgc atgcatggtg
      721 cgtccgatct tggagaaagt taaagcttat cctgatccgg cgcccgggcc gaggttgcgg
      781 ccggtggcac cgcgccgccc ttttgccgtt cgcggaacag ggtggcgcgc atcaggaaga
      841 tcgtcgtgac gggcgccgtc agggctacaa acagcgtgat cagcacggcg cgcaggaaca
      901 ggctgtcgcc ctggaccgaa aagtagatga tggtcgccag ggtgatgcac cacacgccga
      961 cggttgcgcc cagggtgggt gcgtggatgc ggtggaaaaa ggtcggcagc cgcaccagcc
     1021 cgaaagagcc gatggctgca aaggcggcac cgaccacggc aaacacggcg gtgaggactt
     1081 cggcccacag cggcaagccg gtgctcattc gatcacctcg ccgcgcagca gaaacttgcc
     1141 aatgacggtg gagccgaaaa aaccgaacag cgcgatcagc agggcggttt caaagtagac
     1201 gttgctggcg tacacaatgc ccagcaccag catcatcagc atgccgttga tgtagaggca
     1261 gtccagcgcc atcacgcggt cctgcgcggt cggtccgatc agcagccggg cgaccgcgca
     1321 cagcatggcg acggccagca ggaagagggc gagtttgagt ccccagaaaa gaaagtcgct
     1381 catgattcga aaatctccat caacggggct tcataacgcg tcttgatcag cgtgatgaaa
     1441 gccgcctcgt ctttcacctc aaatacatgc agcaacagcc tggaacggtc aaacgacaac
     1501 tctccccagg cggtgccggg tgtcaggcag cagatcatgg ccagcatggc cagggcattc
     1561 gggtcgcgca gctgcagcgg gatctccacg aagtgggcct cgatgtccgc cgtgcgccgc
     1621 gtcagcagca gccgggcgac cgtcagcgcc gaatgcagca ggtcgcccat cacccgcacg
     1681 accaggcgca gggcgacacc tggtttgcgc atgcgcacgg tggcggggcg caggctgcgg
     1741 gtcagcagag gtaccgccac ggcaaaaaac gccgccatca gcaaggtggc cgcatcgagt
     1801 gtctggttca gcagcagcca gacgacgaac agcgccagcg acagcggcgg cgaaggcaac
     1861 cagcgccgca tcatggcgag accttcgggg tgggcggatt tggcacctgc cgggcgccca
     1921 tcacggcgtt gcggtaggcg gcgggtttga acaggttttg cgccatggcg ttggcgtgct
     1981 gcatgaccgg gccggcctcg atgctcaggg cgacgctggc ggccagcagc gcggcgaccg
     2041 gcagcacctc gagggcgcgc aaggccggca tgctgctcac gggctgcgtc cagaagtagc
     2101 ggatgccggc gcggctcaac gcgaccagcg tcaggaagcc ggacagcagc agcagcgcca
     2161 gaaagctcca tttcgccggt gaaatcacgg gtgtgtccag cagggccgac aacatggcga
     2221 acttgccgac aaagcccgac agcggcggca ggccggccag cagcagcgtg cagaccacga
     2281 aggacaggcc cagaaaggca atgccggccg ggatctcgcg gccgtagagc gcctgctgtt
     2341 cgtcgtcgag gttcacgttg tcgatggcgg tcaggtcttc cgagagaaag ggcgcgttgc
     2401 ctgctttctc atgcggggcg atgctggcgc ccgcattgcg ccagcgttcg atcatgtcga
     2461 tcagcaggaa gaaggcgctg gcggccagcg tcgagctgag caggtaatac agcgcgccgg
     2521 cccagaccgc aggctgtccc aggccgatgg cggtcagcag cgtgccggcg gagatcagca
     2581 cggcataccc ggccaggtgc gacaggcgct gcgtgccggt gatgcccagc gcgctgacga
     2641 acagcgtgga caggccgatg gccagcagca cctcctggcc aaactgcgcc gacgcgccgg
     2701 tgtcgggcgc gaacagcacg gtccacaggc gcagcacggt gtagatgccc agcttggtca
     2761 gcagcgcaaa gaccgcactg acgggggcca ccgccgcgct gtaggccggc accagccaga
     2821 agttgagcgg ccaggcgccg gccttggcaa aaaaggcggt ggccagaatc gccgcggccg
     2881 cgtgcaccag accgcgatcg gccgcatcga gcttggcaat gcgcaggccc aggtcggcca
     2941 tgttgagcgt gccggtcacg ccgtacagca gcgccgcacc gaccagaaac agcgacgagg
     3001 ccgccaggtt gatggcgatg taatgcaggc cggcgcttac ccgcaagcgc cccgagccgt
     3061 gcagcaacag gccgtaagag gccgcgagca tgacctcgaa aaagacgaac aggttgaaca
     3121 ggtcgccggt caggaaggcc ccgttgagcc ccatgagctg cagctgcagc agcgggtgaa
     3181 aatgcacccc cgcacggtcc cagcgcgaag tcgaataaat cgacacggcg aaggcgatca
     3241 cgccggtcag cgccaccatc agcgtggaca ggcggtcggc caccagcaca atgccgaagg
     3301 gtgcgcgcca gttgcccggc aggtagacac cgatggagcc gggtccggtg ccggtgtcca
     3361 ccgcattgac ccagcgcagc agtgccagcg cggccagcag gccgatcagg ccggagacca
     3421 cggtgagcgc tgccttggtg cggcgccggt gttcgcccag cagcagcatc agcgcggcgg
     3481 tcagcatggg aacgagaatg ggcacggcca ccaggtgcgg catggtggcc tcgagcaggc
     3541 ggtccagcca gtgcaggagt tcactcattc gggcgctccc ttcacatgca cgctggtgtc
     3601 atcccccgcg tcgtccttgc cgtccacatg gtcggtgccg gccaggccgc gcgaggccag
     3661 catcaccacc aggaacaggg ccgtcatcgc aaaaccgatg acgatggccg tcagcgccag
     3721 cgcctgcggc atcgggtcgg cggtgttggc cagcgtggcg gtgatgccgg gcaccagcac
     3781 cggctcgctg tcaagcttga ggcggcccat gctgaagatg aagaggttga ccgcgtagga
     3841 gatcagggtc aggcccatga tgacctggaa ggtgcgcggg cgcagcagca ggtacacgcc
     3901 cgagctggtg agcacgccga tgccgagggc gaggacgatt tccatcaggc gggttctcct
     3961 tcggagcttg ccttcgcttc ggcagcggcc tggcgctcgt tttgctcttc gacccagcgg
     4021 tggctgcgca ccgattggtg ggccagtgcc gtgagaatca gcatggtgga ccccagcacg
     4081 atagcgaaca cgccgaggtc gaaaaacagc gcgctgggca cgtgcaggcc gcccagcagc
     4141 ggcaaatgca ggtgggcggt gtgggtggtc atgaaggggt aacccagcac caccgcgccg
     4201 ccgccagtgg ccagcgcaag cagcaggccg agggcgatcc agcggcgcgg gtagatgcgc
     4261 aggtgttctt ccacccactc ggtgccggat acgatgaact gcagcagcag cgcgatcgac
     4321 atcaccaggc cggcgacaaa accgccgccc ggcgcattgt ggccgcgcat gaagaaatac
     4381 gccgacacca gcaccgacac cggcagcagc aaacgcacca gcacggccgg caccatcagg
     4441 tagccgacag cggtgtccac cgcacggcgc gggttcagca ggtcgctgct gccgtcgtcg
     4501 acctgcgcgc gctgctgcgc cggcagggcc atggactcga tcgctgggcg gaagcggcgc
     4561 agcagcgcgt acaccgtcag ggccaccacg ccgagcacgg tgatttcgcc gaaagtgtca
     4621 aagccgcgga agtccaccag catcacgttg accacattgg taccgccgcc ctcggtgagc
     4681 gcgcgcttga gaaagaaggg ggaaatgctt tccggaaaac gccgcgtcat caccgtccat
     4741 gacagggctg ccatgccggc gccggccaaa acggcgatgg tcaggtcgcg cccgcgccgg
     4801 ccccacagcc gaaggcgcgt ctgcatgggc tggatggcca ccttgcgcat ggggagccag
     4861 cgcaggccca gcagcagcag caccgtggtg acggtttcca ccgccagttg ggtcagtgcc
     4921 aggtcgggcg ccgaaaacca gatgtaggtg atgcagcaca ccagaccggc acccgaggcc
     4981 agcatcagcg cggccaggcg gtggaacttg gcctgccagg cggcggcgac ggcacaggcc
     5041 gcgcctatga cccaggtcat ggcaaacatc ggtgaaaagg ccagcagctc gcgtttgccg
     5101 cgtacggcag gcgtcagcca cagcgcggcc gcggcacccg cgagcgcgat caccagcagg
     5161 agcagcagtt gagattgcag gcgccgcgta cccagcaggt agcggctgcg ccggccggcc
     5221 tcgctgacgc gcgccagcac gttctcgaaa atgcgctgac cgtcaaaacg gtgcagcagc
     5281 ggtgtgtgcg ccagatggcc gagcgcgcgg ccgcggcgct gtagcaggta gagcgcggcg
     5341 ccgccggcca tcgcgacaaa actcatcagc agcggcaggt tcaaaccgtg ccagaccgcg
     5401 aggctgtagg ccggcaaggt gccgcctacc accggccggg ccgcttcggc cagcagcggg
     5461 cccaccgacc agtgcggtgc aacacccacg accaggcaga ccagcaccag cagctcgacc
     5521 ggcacacgca tccagtgcgg cggctcgtgc ggtgtgcgcg gcacatcggg tccgcagggc
     5581 gggccgaaaa aaacgtcgaa aacaaaacgc gccgaatagg ccacgctgaa catgcccgcc
     5641 agggtggcga tgacgggcag gccccagttg agccaacctg tggactggat atacaccgtc
     5701 tcggcaaaaa acatttcctt ggacagaaag ccgttgagca gcggcacgcc cgccatcgag
     5761 gcgctggcaa tcaccgccag cgtgccggtg atgggcatca gccgcatcag gccgctgagt
     5821 ttgcggatgt cgcgcgtgcc ggtttcgtgg tcgatgatgc cggccgccat gaacagcgag
     5881 gccttgaagg tcgcgtggtt catgatgtgg aagaccgcgg ccaccgcggc caacggactg
     5941 ttcagcccca gcagcagggt gatgaggccg aggtgggaaa tggtcgaata cgccagcaat
     6001 gatttcaggt cacgctggaa catggcgatg aagccgccca gcaacagcgt gatggcgccg
     6061 gcgccgccga ccagccagaa ccattcactg gtgccggaga gcaccggcca caggcgcgcc
     6121 atcaggaaca ccccgagctt gaccatggtg gccgagtgca ggtaggccga caccggggtc
     6181 ggtgcggcca tcgcgcgcgg cagccagaag tgaaacggga actgggcact tttggtgaag
     6241 gcgcccagca acaccagcac cagcaccacg gtgtacagcg gatgcgcccg gatctcgtcg
     6301 cccgaggcca gcaccaggtc caggtcgtag gtgcctgtga tgcgccccag taccagcaca
     6361 ccggccagca ggcacaggcc gccggcgccg gtgaccgtca gcgccatgcg ggcgccgcgc
     6421 cgcgcgtcgc gccggtggtg ccagtaaccg atcagcagga aggaaaacag gctggtgagc
     6481 tcccagaaca tcaccatctg caccaggttg ccggacagca ccacgcccat catggaaccc
     6541 atgaaggcca ggaaaaacga gaagaagcgg ggcaccgggt ccgatgccga catgtaataa
     6601 cgcgcgtaca gcacgaccag cgcgcctatg ccgagcacca gcatgcagaa cagccaggca
     6661 aaaccgtcca tgcgcagcac gaggttcagg ccaagcgccg gcacccattc gatctcttcc
     6721 cggatcacgt tgccgccggc cagctgcggg aacagccagg ccacctgcaa ggcgcacccc
     6781 agggcgacca ggcctgccag cgtcgattcc cggttgcggg cgttggccgg taacagggtg
     6841 gccagcacgc tggcaataaa gggaagggcg acgagcagga cgaggggcat ctcgcctgat
     6901 tctatagagc ggccctggtg caggccgccg gcaaaccttt gcgctgtcca aggcctttgg
     6961 caggccgatg cctggcctga cgctggcggt gaggttctag cggctcatct gctgcggcag
     7021 ccaggtgacg atgcccggaa agacatagac cagggtgatg gccgcacaca tcaggaaaaa
     7081 catcggcaag gtgaccttgg cgatccaggg cagctcgcgg ccggtcatgc cctgcagcac
     7141 aaacaggttg aagccgaccg gcggggtgat ttgcgccatc tcgacgacca gcacgatgaa
     7201 gatgccgaac cagatcgggt cgatgccggc tttctggatg gtgggcatca gcacgcccat
     7261 ggtcagcacg accatggaga taccgtcgag aaagcagccg agcacgatga agaagaaggt
     7321 cagcgccagg atcagctggc cctggctcag gcccagcgag gcaatccatt cggccaggtg
     7381 gcgcggcagg ccgatgtagc ccatggacag cgtcagaaag gccgcgccgg ccaggatcag
     7441 cgcgatcatg cagtaaagcc gcgtcgcgcc catcagggct tcgcggaagg tgctccagct
     7501 catggagccc tgcagcgcag agatgaccag ggcgcccacc acgccgatgg ctgccgcctc
     7561 ggtggccgtg gcaatgccgg agtagatgga gcccagcacc gaggcgatca gcagcaccac
     7621 cggaatcagc gcgcgtgagg ccgagagctt ctgcagaaac gtcgtttttg cgtcggccgg
     7681 gggaatctgg ccggggttgc gcagcgccca gaccacgatg tagcccgaaa acagcaaggc
     7741 cagcaggagg ccgggcaaca cgcccgcaat gaacagcctg gcaatcgaga cctcggccga
     7801 gacgccatac acaatcatga tgatggacgg cgggatcagg aggcccaggg tgctggcgcc
     7861 ggccagcgtg ccgacaatca tgtgttccgg atagccgcgg cgcttgagtt caggcagcgt
     7921 catcttgccg atggtggcgc aggtggccgc gctggagccc gacaccgctg caaaaatggt
     7981 gcaacccacc acgttggtgt gcagcaggcg tccgggcaag gcctgcatcc agggcgccag
     8041 gcccttgaac atgtcctgcg agagccgggt gcgaaacagg atctcgccca tccagacaaa
     8101 cagcggcagg gcggtcaggg tccagcttga ggcggagccc cagatggtga cggccatggc
     8161 gtcgccggcc acacgggtgg tgaacagctg catgcctatc caggccacgc ccgacagcgt
     8221 caggccgatc cagacgccgg tgcccaggat gaagaagagg gagagcacca gcatggccat
     8281 gatggtcagg tcgttcatgg aatccttatt cgttgcgcag cgcttcgctg ctggtgacgg
     8341 ctgttcggcg gccccggatc tcgagcacca gctcgtcgat gaaggcaatc accaggatga
     8401 ccgcaccaaa ggccatggca agctggggta tccagagtgg cgtggcatcg cttccggtgg
     8461 agatgtcgtt gaacacgcgc gactgccagg ccagcttgca gctgtagacc gcaaacagca
     8521 gggccagcaa ggtggccgcg gccagggccc agagttccag cgcttttttc cagcggcctt
     8581 tgagggcgtt gaccagcagc gtcacgcgga tgtgttcgcc gcgcttgagg gtgtgcgcca
     8641 gcgcgaggaa gccgctgccg gccatcaggt agcccgcgta ggcgtcggtg cccggaacat
     8701 ggaaatgcag ttgccggctc acgatggaca gcagcaccat gaccagcaga cccaccaaaa
     8761 aaagcgccgc cagggcggcg gcgctgttgt aaatgccgtc tagcagcttg cgcatcgcgg
     8821 ctatttattt gcggtaggcg tcaaccagcg cctggccttc agggccggct ttgtccagcc
     8881 attccttgag catcacgtcg ccgacttttt tcatgtcggc cttcagggcc ggcgagggcg
     8941 tgacgatgtt catgccgttg gccttgagcg tgtccagcga cgcggtgttg acacgtttgg
     9001 acgctgccca gccgcgcact tcagcctcgg cggccgcctt gagcacggcc tgtttggtgg
     9061 ccgcgtccag cgcgttaaag gcggccttgt tcacgatgac cgcatttttc ggcaaccagg
     9121 cctgggtgtc gaagaagaac ttgaggtgtt cgtaggtctt gctgtccacg ccggtggaac
     9181 ctgaggacat gtaagactcg accacgccgg ttgccagcgc ctgtgacagc tcggaggcct
     9241 ggatggtcac gggctgggca cccaccagct cggcgatacg cgccgtggcc gggctgtagg
     9301 cgcgccattt cacgcctttg aggtcggcgg cggcgttcag ctgcttcttg gcatagatgc
     9361 cctgcggggg ccaggccacg gcgtaaagca gggccatgcc ctgttcgccg agtttctttt
     9421 cgagcagcgg tttttgcgcc tgatacagct tcatggccga gtcgtagctg tcggccagaa
     9481 agggcagccc gtcggcgcca aacagttgcg actcgttctg gaagttgacc agcaggattt
     9541 cgccggcctg ggcctggccg ccctgtacgg cacgcttgat ctcgggcgcc ttgaacaagg
     9601 atgcgttggc gtggactgtg atcttgagct tgccaccgct ggccttgtcc acgtcattgg
     9661 cgaactgcac cagattttca ctgtggaaat tggtggcggg gtaggcggcg ggcaggtccc
     9721 acttggtctg ggcaaaggtg gcgccggtgg caagggccag gctggtggca agggtcaaga
     9781 ggcgaagctt catggtgact ccattggttg agttgatggg acggacttta cgcggctaaa
     9841 gaggggatga tgatgggtgt ttcccctaag tgtcaacaat tcgttgataa attgtcattc
     9901 atctgcctac aatctgccgc atgcaacatc aatgccaaca ggagtggggt catgtcaaaa
     9961 aagccggtcg ataaggctgc gagcccggag cgctcgccca ttgtttcttc tgcgcacctg
    10021 gtatcgccgc acagttcgga gatgagtgag tttgaattcg ggctgatcgt ggcgggcaac
    10081 gcctttcatc ggtggatcgt gcactgtatg agtgctgccg gcctcaagga cctgacgccg
    10141 ctcgatgttc tggtgctcca ccatgtgacg caccgcgcgc gagacaagcg gctggcggac
    10201 atctgtttca tcatgaacgt cgaagacacg cacctgatca actattcgct caagaagctg
    10261 ctcaacctgg gagtggttga gtcgagcaaa aacggcaagg aggtcaccta cgcatcgacg
    10321 gaagcgggcc gtgcccatgt gcagcgctac cgcgagatcc gtgagtcctg cctgattgat
    10381 gcgatgaatg cagatgacgg attgaaccgc gat
//
LOCUS       DAIDFG010000084        10349 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_84_length_10349_cov_18.321741, whole genome shotgun sequence.
ACCESSION   DAIDFG010000084 DAIDFG010000000
VERSION     DAIDFG010000084.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 10349)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10349)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10349
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84_length_10349_cov_18.321741"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1946 a         3253 c         3257 g         1893 t
ORIGIN      
        1 acgctgccgc ccttgacggc cacgctggag gcggtcactg ccagctggtt ggcctggcgg
       61 gcgttgtcgg cgttttgttt gacggtgctg gtcagctctt ccatcgatgc ggcggtttct
      121 tccaaggagc tggcctgctc ttcggtccgg gacgacaggt cgtggttgcc ggcggcgatc
      181 tggctggagg ctgtggctat cgtgtcggtg ccgctgcgga cttcgccgac caccttggcc
      241 aggctgtcgt tcatgccccc gagggcttgc atgaggtcac tgacttcatc cttgcctgtc
      301 accttgaccc gcgacgtcag gtcgccacca gccactgctt gcgccagcaa cagggcttcg
      361 cgcagcggat gggtgatgct gcgggtaaag acccaggcca acgcggcgcc tatggtcaat
      421 gcggccagac ccaggccaaa cagcagaacc gacagctgct ttccggcagc gacggcctcg
      481 tccagcgttt caacaacgtt ggcttgctcc aattcaacca gcttgttgat ctcgaccatc
      541 gctttctggt gcgccggttc aatgcggccg gcaatgattt ttgccgcgcc ttcgggatcg
      601 aaagccaggg cctgtgcgac agcctcgagg aagggtgcct cgatctcctt gtcgagccgc
      661 gcaatctcgg cgaccatttt cttttcggct tccgagaggt cggttgccag cagcttgtcg
      721 cgcgcctggg cggaacgctt gcgttcagcc ttgccccttg cctcctgcgc ctgcatggca
      781 ccgacgtccg actgaagggc gatgttgcgt atggcgatcc cgccttcgag caaggcgctg
      841 cgcatggtgg cggccaatgc gcttttcgca ttggctttct ccagcccctc gatcattacc
      901 gtcctgtttt gcagaccgat ccaggtgttg gtaacgagca ccaggacaag aatcatcagg
      961 atgatgccga acccgatgcc aagacgcgcg ccgatacgca aactacgtaa attcatgatc
     1021 actccaaagc taacgtactg ttggttcctg cggaaagcag gcatgcaacg cggcaggggc
     1081 ggctgcgaat caaccacgcc cgtccattgt caatttgtaa aaagcctgtg ctgccgcatg
     1141 gcggcctctg ccatccgatg gaatggcgcc gtcatgcggc atggacggtt cttatttgta
     1201 gatgccgccc atgaagatga tgtcgtcgac cttttcgacg taagacgatt ttggctcgac
     1261 ggccttggtg atggggttgg gccacttgta atcgacccag cctttgccct tgccgccggc
     1321 tacttcgaca aagcccttga cgaaatattt gccatcgaca tccttgagtt cgatgaggct
     1381 cttgtcgatg agcttggcgt tcgcgccgtg ggcgagcacc ttgccgttca tgtccaggac
     1441 ggtaatgtac agatcgcggt ccttgaactg gccattcgga ttgctgattt ctgcgaaagc
     1501 cttgtccttg ccgttggctt tcatgtacgc ggctgccttc ttgacgagcg cggtggcttc
     1561 ttcggccgtt cctttgtccg gggcagccac ggccccgatg ttgaatgaca gtgccaacag
     1621 gccgaacacg attccgtgga acagtttttt catgattgaa tttccttgat ctctaaaaaa
     1681 tccggactgc cggacaactt gtaaacccca tttttcgcaa gcactgcaag cagtcaccag
     1741 agcctccacc tcgtctgcct cgttccgaca agaggacggt tcccgtatcg cagccacccg
     1801 cacttgacag cttgttgaca gctgctttca tggataacgg caatcctttt ggaatgttga
     1861 gggtatctac gggttttcct gcatgctcca ggcgccacgc cggccccggg tcacctgaaa
     1921 cacctccagt tcctccgccc cttcgcgggc agcccctaca ctgcagggca tgaaaaccgc
     1981 cgacattgcc ccgttttttg ccacgctcaa agcctccaac cccctgccgg tgaccgaact
     2041 ggaatacagc agcgtgttcg agctgctggc ggccgtgctg ctgagcgccc aggccaccga
     2101 cgtgagcgtg aacaaggcca cacgcaaact tttcccggtg gccaacacgc cgcagaaaat
     2161 tctggacctg ggccaggaag ggctggaggg ctacatcaag accataggcc tgtaccgcag
     2221 caaggccaaa cacctgatgg aaacctgccg catgctggtg gagaaccacg gcggccaggt
     2281 gccgcgcagc cgcgaggccc tgcaggcgct gcccggcgtg gggcgcaaga cggccaacgt
     2341 ggtgctcaac tccgcgtttg gcgaggcgac cctggcggtg gacacgcata tcttccgcat
     2401 gggcaaccgc accgggctgg caccgggcaa gacgccgctg gaagtcgagc tgaaactgct
     2461 caagcgcatt ccgcccgagt acctggtgga cgcgcaccac tggctgatcc tgcacgggcg
     2521 ttatgtgtgt ctggcgcgca agccgctgtg ctggcagtgc gcggtagcgc cgtattgcga
     2581 ctacacgccg aagacgccgc agccgggctg aggccggggt ggccgttgcg gacatgaccc
     2641 gaatggaact tacataattc aaaaaggctg tcatcccggc tggcgggttt cccggtattc
     2701 gcggcttatg caagtgccat tcgacttgac gccgcccaga tctgcacccc agcgcaggtg
     2761 gtctgcgccg catggatccc ggatcaggtc cgggatgaca actccgcaag gcatcccccg
     2821 cctgtcattg cgggcttgac ccgcaatcca tgctacgccc gccagcgacc gtggttcatg
     2881 caacatggat cccggatcga gtccgggatg acaaacggga gccaggatga caggctgggg
     2941 gccgggatca caattccagc aacaatgcgc catggactac ttcgtctatc tcatggccag
     3001 cgggaaaaat ggcaccttgt acatcggcgt tactaacgat ctggttcgcc gcgtgtatga
     3061 acacaaaaat gcaaccgtcg agagcttcac aagccgctac gctgttgatc aactcgtgtg
     3121 gttcgagatc acaccatcca tcgaagcggc catccacaag gaaaagcagc tcaagaactg
     3181 gaagcgagag tggaagttgg cgatgatcga gaagtccaat ccggagtggc gggacctgta
     3241 cgcgacgctg ctttgacgat acatgccctg cgaggctcac ttggctaccg accacgcagc
     3301 tacgaagatt cactcctgtt ttgatagctg ctcgcgcttg ccccgcctgg gctggaggcc
     3361 gatttgatat caagcctcca tgaaaaacgc cgcccagggg gcggcgctca tcggttcacc
     3421 ggcaggccgg gcgcggctca gaacagcatg ttgggcagga acatggtgat ggctggtacg
     3481 taggtgatga tcatcaggaa cagcagcagc accagcgtcc acggcagggc cgcgcgcgcc
     3541 acgccgagaa tggacatttt ggtgatgccc gcggtgacaa acaggttcag tcccaccggc
     3601 ggcgtcacca tgcctatctc caggttcacc accatgatga tgcccaggtg aacggggtct
     3661 atgcccagcc gcgtggcaat gggaaacagg atgggcgcca tgatcaggat gatgccggtc
     3721 ggctccatga acatgccggc gatcagcaac aggatgttga ccaccacgag gaactgccag
     3781 gccggcatgc cccagccgat gatggtttcg gcaatggtct gcggaatgcg ttcggtcgtc
     3841 agcacatgtg caaacagcag cgcattggcc acgatgaaca tgagcatgat ggtgaccttg
     3901 ccggagtcca gcaatacctt gggcacctgt ttcatgccga tatcgcggta cacgaacacg
     3961 gcgatgaaga aggcgtagat ggccgccacg gccgcggctt cagtcggcgt gaagatgccg
     4021 ccgtagatgc cgcccaggat gatgacgatc aacagcaggc cccagagcga gtcgcggccg
     4081 gccttcagga tctcgcccag gggcgcacgc ggctggcgcg gcagattgag catgcgggca
     4141 cgcacgtaga tggccagcat gagcatcagg ccgagcatga tgcccggtat gacgccggcc
     4201 atgaagagct tgccgaccga ggtctcggtg gcggcgccgt agaccaccat cacgatggag
     4261 ggcgggatca ggatgcccaa cgtgccggcg ttgcagatga cccccgccgc aaaattctgc
     4321 gggtagccgg ccttgaccat gccggcgatc acgatggcgc ccaccgcaac gaccgtggcg
     4381 ggcgatgagc ccgacaccgc tgcgaacaac atgcaggcca gcaccgacgc catggccagg
     4441 ccaccccgga agtgcccgat gcaggcgttg gcaaaccgga tcatgcggcg cgccacgccg
     4501 cccgtcgtca tgaagttgcc accgagaatg aaaaacggaa tggcaagcaa ggtgtacagc
     4561 tcggaagtct cgaacagctt gagcgtcagc gaggccagcg agtcctgtgc aaacaccagg
     4621 atggtcagga tggacgacag gccgagtgcc accgcgatcg gcatgcccag cgccatgaag
     4681 gccagcagca ggatgaacag tgcagcgatg gtcatgaacg cacctcgccg acggccggtt
     4741 cttccttgag cttgagcgcg tcctgggcct cgtcaccgag cagatgggct tcctggccgc
     4801 tagccagccg ccacagcacc tgcgcaaagc gaaacgccag cagcaggaaa cccagcggca
     4861 ggatgatgcg cggtatccag gtctggatgg gaatgtcctg catcaggatg ccgacgctgt
     4921 aaatcttgta gacatactgg taacttccga aagcaacgat cagggcatac acgatgcaca
     4981 gcgaggccgc gatcgactcg accacgcggg ccggccggtt gcccagcagc ttgaccgcgg
     5041 catcgacgcc gatatgtgca ccgacgcgca cgccgtagga catgcccaca aaaatcagcc
     5101 acgcaaacat gacgccggtg agttccagcg cccagacaaa gctgtagttg aaaacgtaac
     5161 gcgcaatcac ctgcatgaag gtgaccaggg tcatcgccgc catcaggaag gtaatcaggc
     5221 cttcctccaa atgttccagc caacgcatag atgtacaaag ggcccgcaag gcgggccctt
     5281 tccttgtggt tacttgttgg ccttgagggc gccgtcgatc aggtcacgcc cgatgtcgcc
     5341 ttcgaacttc ttccacaccg gctccatgga cttgcgccag gcggccacat cgtccttgga
     5401 cagcttctgg attttcgcct tgcctgcgtc ggcaattttc ttgcggtcgg cttcgttgat
     5461 gtccagcgcc agcttgttgg catgggcagt ggcttcggtc atggcttcac tcagaccctt
     5521 gcgcacgtcg gccggcaggc cgtcccacca cttgacattg gtgacgacca tgtagtcgat
     5581 cactccgtgg ttggtttcgg caatggtctt ctgcacttca tggaacttct gcgaatagat
     5641 gttcgaccag gtgttttcct ggccatcgac cacgccggtc tgcaaggcct gatagacctc
     5701 ggcgaacgcc attttctgcg ggttggagcc cagcgcgcgg aactgtgcct cgagcacgtc
     5761 ggaggcctga atgcggaact tcaggccctt gacgtcatcg gggcgaacca gcttgtcctt
     5821 gttggtggac agttgcttca tgccgttgtg ccagtagccc aggccaagca ggccacggcc
     5881 cttcatggcc tccagcaaag ccttgccctg cgggccggac tggaagcggt ccaccgcctc
     5941 gatgtcgtca aacaggaaag gcagatcgaa gacctggatc ttcttggtgt agcggtcgaa
     6001 cttggacaac gacggcgcga tgaactgcac gtcgccgagc agcagcgctt caagttcctt
     6061 ggcatcaccg aacagctgcg agctcgggaa cacctgcacc tgcaccttgc ccgggagttt
     6121 tttctcggcg agctccttga acttcagtgc gccctgccct ttgggtgtct gctcggccac
     6181 cacgtggctg aacttgatca cgatgggggc ctgggccgat gcggcgaacg gcagtgcaag
     6241 ggcgccggcg gcaagcgccg tggcaagaag ggtgcgacgc aaacgatgga ttgttttcat
     6301 gatgtctcct ggttaaaaaa aggtgcggat tgagcatccg tctggatggg aatataggct
     6361 tgtcagcgct cgatcaccat acgtacaaac cgatgcctgg catggttttt cagcagcggc
     6421 gcggcgcgtc ccgtcgggtc tcccactggg gaaatgcccc gtgtcgccgc ttggaggtac
     6481 agcgcccatc ttgcggcgag tggctccaga aaacattcgg acaaaccctt ctcaggcacg
     6541 gtcaggccct ccatgcccat gagatacatc ggctgcacgg gccagcgtgc gctgcgcgag
     6601 cagcaacacc ggcgcatcca ccatgcggcc gtcgaggctg accaccgcac cgcctgcggc
     6661 ctcactggca gccaacacac gacgcgccca gtcgatctcg gccgggctgg gggtgaacgc
     6721 cgcattgacc gccgccacct gcgcgggatg aatacacagc ttggcaccaa agccgccgct
     6781 gcggctgcgc agcaaatcgc gcgcgagttg cgccgcgtcc tgcgtgtccg ccgtcacgcc
     6841 gtcaatcggt gaagggagtt gcgcgcggcg cgaagccagc agcaccgcca gccgaaccgc
     6901 cgtcagctcg gcttcgtcgg gtccgcaagc caggccggca tcggcctgga agtcgaggtt
     6961 gccaaaggcc agccgcagca cctgcgggca acgcgccagt gtgtcggcgg catcgaggcc
     7021 ggcaaccgat tcaatcagcg gcaccagcac gcacgcagag cccagcaccg ccgcgacatg
     7081 cgagagctgg gccgcatgtt cggccttggg cagcaccacg cccgccacgc ccagcccgac
     7141 cagcaggttc aggtcgtcgc catgccaggc cgtgttgcct gcattcacac gcaccagcac
     7201 acgtgcgcgc tctgccattg cgaggccagc ccaggcctgc gccagccctt gccgcgcctg
     7261 cgccttgtcg ccgggggcga ccgcgtcttc gaggtcaatg atgacggcgt cggcgcccga
     7321 ggccaatgcc ttggcaaagc gctcgggccg ggtggcgggc acgaacagga aggagcgggc
     7381 cagcgccagc ggctggccgg cggactcgct catgcctcgc cttccagcag caaacccacg
     7441 cgcggcaatg acgcgacctg ccacaaggca tcgatggctg cctgcatctc gcccggtgtg
     7501 gcgccgccgc tgaaggcggc caggcgcagc gccttggcgg tgatttcttc gcggctcagg
     7561 gtgttgccgg ggtcgccctt gggttcgtcc accctgccct gcagcacgcg gccatccagc
     7621 gtggtgacgg tgaccttgcc aatccagcgc tgcggatagg ccgcgtccac ctcggcgtcc
     7681 agcagcatcg tgacccggtc gcgcaggacc gtggtcgcgt cttcgcggaa atgtttgtcg
     7741 aactcgacca ggccggcatg gccgaaccgt gcgaccaagg ccagcaccgt gcccatggag
     7801 aacttgctct ggtgcaccgt ggccgggtcc agcaccgggc ccagcacgtc gatggcgccc
     7861 tggtgtacat gggtgaccac actggcgatg tcggccggtg ccagctgatg gacctgcatg
     7921 acctgcagca gcgcatcggc cgccgggtgg gtgtggcggc aggacgcgtg gtatttgaag
     7981 gaggtctcgg ccgtggccca gcgtgtcccc agggcgtcgg tgagtttggc gggatcagca
     8041 tcgctggaca tgccggcggc catgccttgc gggccttcga gaatctgcgc cgcgccggta
     8101 aaaccgtcca gcgccaggca ggccgacatc aggccggcgg cggccgcgtg agcggtgtgc
     8161 aactgtttgg agtcggcggc cgtgcgcaaa aactcccaga gcccggccga ctgcgtgccg
     8221 gccgaaccga aggcgtgctg catctgcgcc gcactcaggc ccagcaggct gcccgccgtg
     8281 gccgcggccg ccagcgtgcc ggcggtgccg gtggtgtgaa acaccttgta gtgcgagcgc
     8341 cccagaaact cgccgacacg gatgccgacc tcgtaaccgg cgaccgcggc ggtgagcatc
     8401 tgcctgccgc tgctgccaaa ggactgcgcc atcgccagcg ccaccgggaa caccaccgtg
     8461 gcggggtgga acaccgagcc gttgtgcacg tcgtcctgct cagccacatg cgaggcggcc
     8521 gcgttggcca tggcggcaaa gtaggcgctg gtccggctgc ggttgatcag cacttcggcc
     8581 acgccgccgg tcggccccat gctggcggca aaacgcgtga tggtctcgac cggacgcgag
     8641 cccttgcctg caattgccga gccgaaccag tcgaccagca ggtcttcagc cttgcgcacg
     8701 acgggcgcgg gaatggcgtc gaaagtcagg ccggcggcga aggcggcgag ttgggcggtg
     8761 gggctggtag cggttggggt catctctgtt cctcagtctc aaaaatcaaa cgtgacacga
     8821 ttccagactt ctggacttct gccttgtcat cccggacctg atccgggatc catgcaacaa
     8881 gacccacggt cgcgggaaag tgccacatgg attgcgggtc aagcccgcaa tgacagtcaa
     8941 tggaaattca tgcatccaat atggctccag tccttatgga taaagcgcaa gcagctatca
     9001 attaaatagc gcccgtctgc tgcagcgcgg caacggcgtc atcgccctgc cccagctccc
     9061 gcagaatggc gcgggtgtgc tggccgacgg ctgggatagg gtccatgcgg tagtcgaagc
     9121 tgttgatgcg gcccggcggc agcaacgcgg gaatgtcgcc gaccggcgag gccacctggc
     9181 gccagcggtc acgcgccttg agctgcggat gcgcccagag ttcggccatg ctgttcatgc
     9241 gggcgttggc aatctgggct tcgtcgagcc gggcggtcac ctcggcggtg ctcatgccgg
     9301 caaaggccgc aaggatgatg gccttgagcg cctcgcggtg ctcgttgcgc cgcgcgttgc
     9361 tgtcaaaccg cgcatcggtc gccaactccg gctgctgcaa cacgaccttg cagaaaatca
     9421 cccattcgcg ttcgttctgc agcccgagca tgacagtgcc cccgtcaccg gccgcaaacg
     9481 gaccgtaggg atagatggtc gcgtgggatg ccgcgctgcg cggtggcggt ggggcaccgg
     9541 cgtaggcgta atacataggg taacccatcc actcggccag cgactcaagc atggacacgt
     9601 cgatgtgcga accctggccg gtcttgccgc gctgcagcag cgcagccagg atgctgctgt
     9661 aggcatacat gccggcggca atgtcggcca ccgagttgcc cgccttgcag ggctcgtcgg
     9721 gcgtgccggt caccgacaga aaaccggctt cgctctggat cagcaggtcg taggctttct
     9781 tgtcgcgata agggccgtcg tcgccataac ccgaaatgtc gcagacgatc agtttcgggt
     9841 tggcaacacg gaggtcgtca tagcccaggc ccatacgcgc ggccgcaccc ggcgcgaggt
     9901 tttgcaccag cacgtcggcg gtttccagca gctgtttgag cacggccagt gcgccgggtt
     9961 gcttcaagtc cagcgtcagg ctttccttgg agcggttcac ccagacaaaa tgcgaggcca
    10021 tgccgtccac gcgctggtca taggcccgcg caaagtcgcc gacgccgggt cgctcgacct
    10081 tgatcacgcg tgcgcccaag tcggcgagct gcctggtgca gaacggcgcg gcgatcgcgt
    10141 gttcgagcga gatgacggtg atgccgtcga ggggtcttgt ttgtggagtc attgcgtatc
    10201 cgttggggaa ggttgcgctt cggccgcgtg tttgatcggg cgcttgttgt tcgcatgcgt
    10261 ctgttggccg ggggttgccc ggcggcaact cactttcttt tgcgtcgcca aaagaaagta
    10321 agcaaagaaa aggcgaccct gctgtctgc
//
LOCUS       DAIDFG010000085        10230 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_85_length_10230_cov_17.661425, whole genome shotgun sequence.
ACCESSION   DAIDFG010000085 DAIDFG010000000
VERSION     DAIDFG010000085.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 10230)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10230)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10230
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85_length_10230_cov_17.661425"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1917 a         3376 c         3246 g         1691 t
ORIGIN      
        1 accgcagcca gcgccacggc gagcacgccc atggttttgc ccagcggcgt aacggtcagc
       61 gcggcggcca gcatggcgcc gacgatgaca atcgccgaaa tcgcattggt caccgccatc
      121 agcggcgtgt gcagcgcagg cgtgacggtc cagaccacgt ggtaacccac gtagatggcc
      181 agcacgaaga tgatgaggtt gatgatggtg ggtgacacgg catccatggt cttcttctcc
      241 tgatggggtg gcgctgccgc cggcccctga taaatgtttt tttgagatcc gctgcttact
      301 tgcgcttgac ttcgccgccc tgcgtcatca ggcaggccgc cacgatgtcg tcgtcgaggt
      361 cgatgttgag cgtgccttcc ttgctgatga ccagcttgag gaagtccagc aagttgcggg
      421 cgtagagcgc gctggcgtcg gcggcgacca gcgccggcac attggtctcg ccgatcagcg
      481 tgacgccgtg tttgaccacg gttttgccgg cttcggtcag cgggcagttg ccgccttgcg
      541 gcgcagccag gtcgacgatg acgctgccgg gtttcatggc cttgaccatg tcctcggtca
      601 ccagcaccgg tgcggcgcgg cccggaatca gcgcggtggt gatcacgaca tcggcctggg
      661 ccacgcgttt ggcaacctcg gctttttgcc gctccagcca gctgggcggc atcggtttgg
      721 cataaccgcc gacgccaacg gcggcttcgc gctcttcgtc ggtttcatac ggcacatcga
      781 tgaacttgcc gcccagcgac tcgacctgct ccttgacgct gggtcgcaca tcgctggcct
      841 cgatcaccgc gcccaggcgc ttggccgtgg caatcgcctg caggccggcc acgccaacgc
      901 ccagaatcac cacacgcgcc gccttgacgg tgccggcggc ggtcatcagc atcgggaaaa
      961 agcgctggta cttgtcggcc gccatcatca cggccttgta gccggcgatg ttggcctgcg
     1021 acgagagcac gtccatgctt tgcgcgcggc tggtgcgcgg cgcggcttcc agggcaaacg
     1081 ccgtcagctg ggcgcccgcc aggcgctgca ggccggcggc gtcaaacggg ttgagcatgc
     1141 cgaccagcac cgtgccggcc ttggccaggc cgatttcgct gtccagcggg caacgcacct
     1201 tgagcacgat gtcggacgca taggcgccgg cagcgtcggt gatttcggca ccgaccgcca
     1261 cataggcttc gtccggcaca ctggcagcca cccccgcacc cgactggatg cacacggtgt
     1321 ggccctgggc cttgagtttt ttggccgtct cgggcgtgac ggcaacgcgt gtttcccccg
     1381 ccgtgatttc ggccggtacc ccgattcgca tgggtgtctc ctcgttgcgt aagtagttct
     1441 acgtgaaatt gtcagctgtc agctagctta catgagtttc cccggctttt ttttgggtgc
     1501 acccctctgg aagtcccgca ggctacggtt cggacagctt gcccacggat aatgctgcga
     1561 tgaatacacg atggaaaccc agtgtcacgg tcgcggccgt catcgagcgc gagggcaggt
     1621 tcctgctggt ggaggaacac acgcaggatg gcctgcgctt gaacaacccg gccggccacc
     1681 tggacccggg cgaaaacccg atccagggtt gcgcccggga gacgctcgag gaaaccgcgt
     1741 ttcacttcac gccgcagtcg ctggtgggca tctatatgtc gcgcttcgaa agaccggtgc
     1801 ccggccagga caccccgctg gacatcacct acctgcgttt tgccttttgc ggcacgctgg
     1861 gcgaggaaat ggccgggcag gcgctggacg aaggcattgt gcgcaccctg tggctgacgc
     1921 ccgatgagat cagggcgtgc gctcacttgc accgcagccc cttgttgctg acctgcatgg
     1981 aagactacct ggcgggcaag cgttacccgg tggatctgtt gaccaccgac cccagcgtct
     2041 acgacgccag accctgaacc tctcgcgaaa tcgcctcacc acctaaaatg ctgcaatgca
     2101 caagcaacgt atcgttgtcg gtttgagcgg cggcgtggac tccgcggtga ccgcgtacct
     2161 gctgaaacag cagggccatg aagtcatcgg catcttcatg aagaactggg aagacgatga
     2221 cgacagcgag ttctgctcgt ccaacatcga ctttgtcgac gccgcggcag tggctgacgt
     2281 gatcggcata gccatcgaac atgtcaactt tgccgccgac tacaaggacc gggtgtttgc
     2341 cgagttcctg cgcgagtacc aggccggacg cacgcccaac cccgacatcc tgtgcaacgc
     2401 cgagatcaag ttcaaggcct ttctcgacca tgcgatgcgg ctgggcgccg aaaaaatcgc
     2461 caccgggcac tatgcgcgcg tacggaaaaa cgagaccacc ggcctgcacg aactgctcaa
     2521 gggcctggac ccggccaagg accagagcta tttcctgcac cgcctgaacc aggcacagct
     2581 gtccagaacc ttgttcccgg tcggtgaact gaagaagacc gaagtgcgcc gcatcgccga
     2641 cgaaatcggc ctgccgaacg cgaagaagaa ggactcgacc ggcatttgtt tcatcggcga
     2701 gcggccgttc cgcgagtttt tgaaccgcta catcgccaag gcgccgggac cgatcaagaa
     2761 cgaccagggc cgggtgctgg gcgaacatgt gggcctgagc ttttacaccc tgggccagcg
     2821 ccagggcctg ggcataggcg gcgtgaaggc gcgtggcgcc gaactcaagg cggtgcaggc
     2881 gctgggccag cgcggcgtcg gcgagcacga gccctggttt gtggcgcgaa aggacatgga
     2941 cagcaatacc ctgtgggtgg tgcagggcca cgaccatccc tggctgctct caacggaagt
     3001 gcaggtgcag gactgcagct gggtcgccgg cgcgccgccg gctgccggca ccatggcggc
     3061 caaaacgcgc taccggcagg ccgacgcagc ctgcgagctg gtggcgccgt cgttgaccca
     3121 tgccgcgctg cgttttgacg aaccccagtg ggccgtcacc ccaggccagt cggccgtgct
     3181 ctaccagggc gaggtctgcc tgggcggcgg cgtgattgca gcgagcaaca caccggcgct
     3241 gccctgagcc cgtcgtccgg ttttttcaat caaatcaggg cctggccctt gatgaataag
     3301 cgccagcagc tactgattta atagcactca cacggttaac cggtcaagga atccgtattt
     3361 caacgaccgt gcgccccggc cggctttcga tggcgatggc cacgccaatc gcgctcgccc
     3421 gctcacgcat ggccagcaga cccttgcgcg gcgcggcggc ggcgtcgaaa ccgcgcccgt
     3481 tgtcaacgac acgcaggcac acaccggcgc cggccatttc agcctcgatc gccagcgtcg
     3541 tcgcctgtga atgctgcagc acattggaca gcgcctcgaa caccatgaac tgcagctggc
     3601 gcatggcatt ggcgtccacc tgggccaccg gctcgagggc gcccaccacc cactgcagtt
     3661 cgatgtccga cgccgcaaac cgcggctcca gccggtaacg caggttggcc agcagcgagc
     3721 tgatgtcgcc cggcagcaaa tgcatggcgt cgatggacag cttgagctgg tccagcgtgt
     3781 aacgcagcgt ctgcaacacg tcgtcggtac tggccttgcc cgactgcagc tgccggatcg
     3841 cggcgctcag gtgcgcaccc acgccgtcgt gcatgtcgcg caggatgcgc gtgcgctcgc
     3901 tgacccgctc ctgctcgcgc gcgagttgtt cgacctggcg gtaggtctgg cccagctcgc
     3961 gctctttctg cgagatgcgg gaagacaggt tggtcatcag gtcgcgcgcc tgcgcgctga
     4021 cctcgcgaaa acgcgtgatc acgatgtagg ccagcgccag cccgaacagc atcgaggagt
     4081 aacgcagcag ggtgttgtcg gcgtaggtcg ggctgatgcg aaaaacgaac cagtcgcgca
     4141 gacccaccag cacattgaca aaaagcgccg cagccaccac ccggtgcgcc ggcgtggcat
     4201 tgcggagcgc acgccagcag aaaaagccga tgaagcaggt gaacgtcagt gcgtaggcgc
     4261 cgtaccaggc cgtcagtacc tgcggcgatg caaacaccag tgcccatgcg atcagcggca
     4321 ggccactggc aaaaagtgtc caggtcatgc ggttgaacca gatcgccggc cggagttcgg
     4381 accagcccgc cacccgcacg gaaaaacttc ccatgcaggt cagccagcct cccgtcatca
     4441 tgaccgtcac catgccccag gccggccagg gcagcggcgg attttcaatc agcacatcgc
     4501 tgacgcgcac cgtccagaac agttcggcca gcccgcccag caggtacatc gggtcgcgca
     4561 cagcgcggta ggcccgcgtc gggtcggtct gcgtgaacca caaggccagc gccaccatgc
     4621 cgaccatgaa gctgagcacc actaccacca gcgtccccgt tgcgcgccag ttgtagtcgc
     4681 tgctgtaaag ccgcctgacc tcctcgtccg gccccatcac cggcgctgcc agcccgccgc
     4741 gccggcccac gtcagcgcgg acatgaatca aaagccggtt ggacggccgc aggagatccg
     4801 tcgcgacggg cacaaaacgg ggcaccttgg cgtagtcgcc cccattgaac tttgtcaggt
     4861 cgccattttt ttccagcaga ttgccgttga gccagatttc ataggcattg cccagcctcg
     4921 gtatatagat ggcataggat ttggcgctgg tgaccggcgg cccgaagtca atttcaaagg
     4981 tggccgcgcc agccagccct ggatgacgcc ggtcccagtg gtagggcagc tcgacgccgg
     5041 actgcaccgg tgccgcaccc tgcagggtga tgagggccct ggcctgacgc agttcctgca
     5101 cctcggcccg ggcgctgccc cagcaaagca gggcgcatac cagcccacac cagagactgc
     5161 gggcgcgcat gtcttcaggc caccacggct cagcgcgcgt cggccgccga cggcggctgc
     5221 agcaggccca gccgggtcgc ctcgtacacc gcctcgcttt tggagtgcac cgcgagtttg
     5281 gtgtagaggt tcttgatgtg ggactggacg gtgtgcacgc tcacactctt gagttttgaa
     5341 atttccgcat aggaaaagcc gcgcgcaatc agctccagga cttcctgctc ccgcacggat
     5401 aacagcgctc ggcccatacc aacggcaggc gtctcgtccg ggccgcgcac caccgccccg
     5461 gccgcagcag cggggggggc ctggtcgacc aggtgcagcg cgcggtactt ggccagcacc
     5521 cggcgcgcga tcatcggcga gatcggcgag gcgccatcct tcatctccag aatcgtgtgt
     5581 gcaatgttgt ccggcgtgaa atccttctgg atgtacccga gcgccccggc ttcgacgctg
     5641 gacaacacgc tgtcttcgtc gccgaacatc gaaatcacca gcgcctcgca cgagggccgc
     5701 aagcgcccgg ccagccggat caggtccagc ccgttgccat cgggcagcgc caggtccgtc
     5761 agcaggatat cgatgacctg gcccttgtcc tcgatcagcg cccgggcttc ctcgaaggtg
     5821 cccacgccgg cgaccagatg cagctgctca cagctagaga cgctgtccgc gaagaagtcc
     5881 cgcgtgggaa catcgtcttc aacaatcagt acgcgccatt cgggcatggg tgaaatcttt
     5941 ggaaggggct gcacagcagc cgatgataaa ggcacgcctc aggaacagca ggcgtgaaaa
     6001 accgcttgag gcatgcgcgt cagcctccac tgttaacgct gtacctgccg gatgcaatcc
     6061 cccgtttatg gggctggccg cgcgcgatcg cctgcccttt gcggcgactg acaggcaacc
     6121 aagtctttca cccttttcag acgggcgcca tcagctcttt ttttgatagc aaatccgtac
     6181 cttacagcac ggtttcgagg taggtataga tgtagttgga gccgccgtcg acgttgccca
     6241 gcaggcgcga ggcgccaaag atcccggaac ggtgtgacac gccgaagccg agataggtgt
     6301 tcttcagcgc gcgtgaaccc accaggtcgc ccaggctgac gtcgatgctc gggtccaggt
     6361 agttgagcag ccgcgaggtc tggcgcccgc gcgccgcctg gctgctggac tcgctgtagg
     6421 gcacacgttg cgcgagcgac accccgaggc ccaggccgag ccgggtcttg acgcgatcgc
     6481 tccacggaaa gccgtagtaa taagccttca tgaacgcgtc gagctgcagc ccgttggacg
     6541 ccagcccgcg gtcgtcgtgt tgcaacaggc cgacgtaacc gacgatgtcc agcggccagc
     6601 cgttgagctg ctcaatgaaa ggcttgccaa tctgcacgcc ggcgatacgt gtcgagttga
     6661 ccttggccgt cgaagcgcag gtcagcgtga tgatcttggc caggtggcag ccgtcttccg
     6721 tggacttgcc gtacagcacc ttgacatggg tgggggaact ttcttcggcc cagtgctgct
     6781 tgtgggtgcc gaaatcgtag gcggcgccga ggaataccgc ggtctgcgcc gaccttccga
     6841 caatcgggct ggccttgacc ttgccgtcca gcacggtccg ggtgatgccg cccagcaggc
     6901 gccatccctc gctcagttca taggtcccgt acaggcccag ggtggtgttg atgccactgc
     6961 cgggctcgta ggcaggccgg gtggccgtgg cttcgtcggg ccgcacgccg tagtaatagt
     7021 tgttcagcct ggcgttacgc accgacaccg tcaggctggg gcgcagcgcc agccggccgg
     7081 agcgccagtc gtagctgtaa gacaagcgca gttcgctgcc ctttgaaaag cggctgacat
     7141 cctgcagata ctcggcctgc agcgtgcccc aaggctggcg gtagcggtaa gacacgccgg
     7201 cgtccaggcc ggagccgcgc tcggccatgc cggccaggct gggaggaagg cggtcgtcgg
     7261 cataaccttc gaagcgctgg tcaagaaaaa gatcgacgcg ctgcgtttct tcgtcgaaca
     7321 gcttcagccc ggcgcgactg ccatgcagaa acacgcgttt gccttcgtag agatacagcg
     7381 gcagcaggtc ataacgcgtg ccgccgccca catagggcga ctgctcggcc cgcacggcga
     7441 aacccaggcc cgcgctgcca ggagcgccga gcagatcgct gagggggtcg gtgttggcat
     7501 gggccggcag tgacaccaac ccgccggcgg ccagcagggc tgcacccagc agcgattgca
     7561 ggcggatcat tgccggggca ttgttttcaa ggtgcttgga gctcatgacg gcgccctgtg
     7621 gttggcaaag ttcgatccta gccaacaatg cgcacccgcg gcagcccaca cctgcaaaca
     7681 aggcaagcgc ctacacagcg ccaaggcgcc ggttgacaaa aaacgttgtt taaaaatgca
     7741 tctgcccggc ctgcgcgaga agcaggccgg gcagaggatc gggagcaggg tttagaacgg
     7801 aatgtcgtcg tccatgtcgt caaagccgct tgcggccttg gtcggcgcag gcgctggcgc
     7861 acggggggcc ggggccggcg cacgcggcgc ggccgccgga cgggaatact ggcgttcgcc
     7921 gccatcgccg tcaccgtcac cgccgggcga acccatgcct tcgcggctgc cgagcatctg
     7981 catctcgctg gcaatgatgt cggtcgcgta tttctcgacg ccgtccttgt cggtgtactt
     8041 gcgcgttttc aggcgcccct cgatgtagac cgagcggcct ttcttgaggt attcaccgac
     8101 gatctcggcc tggcggccga aaaagctgat gcggtgccat tcggtctcct cgaccatttc
     8161 accggccttg ttcttgtact tgctgctggt ggccagcgca atgttggcga cggcgtcgcc
     8221 gctgggcaaa taacgcactt cggggtcgcg gcccaagttg ccgacgagga tgactttgtt
     8281 gacggatgcc atggcgtgtt ctcctaattt ttcacagttt aactggctgc gcggccaggc
     8341 cggcatccgg agcggccgca tgcctgccgg gcgcccgcat gggccacgcc accagcagcc
     8401 acagcagcat cagcccgccg caagccgcaa acagggcttg cggccctgcg gttttggcca
     8461 gccagccgcc cagggcaccg ccggcaaaaa atccgagcga ctgcagcgtg ttgtagaccc
     8521 cgagcgccgc gccgcgggaa tgggccgggg ctacccgtga cgccaggctg ggctgggagg
     8581 cttcaagcac attgaagccg cagaaaaaca ggaacagcaa ggcgcccagc caggccaggc
     8641 tggggctgcc ctgcatcgcc gcgcccatca gtccgaactg caccagcgtg atcaggccca
     8701 ccgcggccag gaacacggcg cgcaggtaac cgcgtttttc caggggaaac agcgtcaagc
     8761 ccatcaccag gaaggaggcc agcaccgccg gcaggtagac ctgccagtgg tggtccttgt
     8821 ccagcccggc ttgcaccagc aacgcgggaa tggccaccca catggccagc tgcacggcgt
     8881 gcagcacaaa cacgccgaca tcgagccgca gcaaggccgg gtgtttcagc acctcggcga
     8941 ggctgccgcg cggccggttc acatgctgcg ccggctcggg cggcacccac cagatcacca
     9001 ccgcgatgcc ggccagcgcc agcaacccgg tgaaagcaaa cagcccgccc aggccgacct
     9061 gtgcggccag caccggcgcc agcaccagcg acagcgcaaa catcagggcg atgctggcgc
     9121 ccaccagcgc catggccttg gtacgcacct cgtcgcgcgt ggtgtcggcc agcagcgcgg
     9181 tgacggcggc cgagatggca ccggcgccct gcagcgaacg gccaatgatc agccaggtca
     9241 ggtcaggcgc ccaggtggcg acaaaactgc ccagcgcaaa caccagcagg ccgaacacga
     9301 tgaccttttt gcggcccagc cagtccgagg ccatgccaaa gggaatctgc aaaatgccct
     9361 gggtcaggcc gtaaatgccc atggccagac cgacacgcgc cgggtcgtcc ccgcccggat
     9421 aacgcgccgc ctccagtgca aacacaggca gcaccaggaa aagcccgagc atgcgcagcg
     9481 caaaaatgga ggccagcgac gcgctggcgc gccgctcggt cgcggtcatg gggtaggaag
     9541 gcaggtcggg gcgggctgaa gacacagagt agggctggaa atttgaaaaa caaaaaaagg
     9601 gctggcaggg gcacctgcgg aagacagggc ctattgtctt tgaattgcac cgcgtttcat
     9661 ttgcccttgc gcaagcacga actgcgcccg caccgggcga caggagcccg ccccatggcc
     9721 ccggaacccc gaaaaacaca catcggtcta tgatggaggg ttttgcggcg cccttataca
     9781 gtgaactctt ccaccgacgg ccaatacctc gctcccacgg ctgctgaaga ccgtgtggca
     9841 accgagcccg ataccagccg ctacctggcc caggccctcg cagcgcagcg catcagcatc
     9901 cgcggcgcgc gcacgcacaa cctcaagaac atcgacctcg acattccgcg caaccagctg
     9961 gtggtgatca ccgggctgag cggctcgggc aagtcctcgc tggcttttga cacgctgtac
    10021 gccgagggcc agcgccgtta tgtggaaagc ctgagcacgt acgcgcggca gttcctgcag
    10081 ctgatggaca agcccgatgt ggacatgatc gagggcctgt caccggcgat ctccatcgag
    10141 cagaaggcca ccagccacaa cccgcgctcc accgtgggca cggtgaccga aatccacgac
    10201 tacctgcgcc tgctgtttgc gcgcgccggc
//
LOCUS       DAIDFG010000086        10180 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_86_length_10180_cov_18.158222, whole genome shotgun sequence.
ACCESSION   DAIDFG010000086 DAIDFG010000000
VERSION     DAIDFG010000086.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 10180)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10180)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10180
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86_length_10180_cov_18.158222"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         2075 a         2873 c         3158 g         2074 t
ORIGIN      
        1 cagatgctgc acaagctggt gtacctgatt gcgggcctag gccttctgca ttttttctgg
       61 atgcgctcag gcaaaaacga ttttgccgag gtgtcggttt atgcggccat catcgcggcg
      121 ctgctgggct ggcgcgtctg gcagcgctgg ggccggcgca agcgttcggc cggtccccat
      181 cctgccgccg caaggcgcct gcagtgaagc aaatgctcct gaatcaggag ctgaagacgc
      241 atgatagata agggctggag catgtttttg cctgaaacct agcgattcac ggcacgcagt
      301 gccggccggg tctggcgtgc gaccgggtcg atggttgccg tcggcaactg gtaagcgcga
      361 tcgtcaaaca ggtcgatgcc gcacatcagg ttttcaatcc agtcgaagaa ttcgttcacc
      421 gcctgcttgc cgacgatcgg tgcgccgtgt tgcggcacca gcatggcgat gtccatgggg
      481 cgcaccatgt tgacccacag ccgcaggatc ttgttcgaga ccatgtagcg ccggtggaaa
      541 ccttccatca ggggtatgtg cggcttgagg ctggtgaccg gctggcgcgc ctgggcgccg
      601 gacatcatcg acacgccgag atcgccggtg aacaggatgc ggctgatcgg atcgtagaag
      661 tgaaaattgc cttcggagtg catgaagtgc gccgggatca accatagttc attggcaccc
      721 agaggcaggc gtccgccacc gtcgggcacg ccgatcacac ggttttcggt cttgccgacc
      781 ttggtgaaat gcggagcaaa acgctcccac acccttgaaa ttaccagcat ggcctgggtg
      841 ctgcttagcc agcgatccag tgaagcgatg atgtcggggt cggcatgtga agcgatgatg
      901 taggacagct tgtgcggcgg aaaatgctgc gtcatgccca tgaacagttc gttgtaggcc
      961 aggttgccgc cgggatcaat gatggccccc gtgccgtggt ccacgatgag aaactggttg
     1021 gcctgaaccg cttgtccatc ttcctcaatg aggtcggtga acatcaggca ggcgtgattt
     1081 ttgtcgcggt aaagttccag agccatgtca gtgcttaacg tcgatttgaa gtaaatcgac
     1141 tctaccccca cccctgcaag ggtcatttga tgagtatcaa gtgacgttgt cctcagggca
     1201 acaatttttc gccgcggaag gtgtcggccg ctgtttcgcg cttgcgtatg agatgggctt
     1261 gttcaccatc gaccagtacc tcggcggcac ggcccctcgt gttgtagttg ctcgccatgc
     1321 tcatgcagta ggcgcccgcc gaaagcacgg ccagacgatc gcctgcggcc acagccagcg
     1381 tgcggtcacg gcctatccag tcgccgcttt cgcagaccgg gccaaccacg tcgtaggacg
     1441 actcctgcct gtcctggtgc gtctcaagcg gcacgatggc atgaaacgcc tggtacatgg
     1501 cgggacgcgg caggtcgttc attgcggcgt cgatcacgca gaaattcttc tgctcaccgg
     1561 gcttgaggta aagcacctcg gtcaggcaga cgccggcatt gcccaccagc gagcgacccg
     1621 gctcgaccat cagttttttg ccgccatggc cgcgtgcatc cagtttggcc agcagttcct
     1681 gccagagcac atctgccgcg ggcggtgcgt cctggttgta attgatgccc aggcccccgc
     1741 caaagtcgat gtgttcaatc gtgatgcccg ccgcttctat gctgtcgacc aggtccagca
     1801 cccggtccat cgcgtccagg taaggggtgg tttcggtgat ttgcgaaccg atgtggcagt
     1861 cgatgccgac tacctgcagt ccttcaagcg acgccgcgta tcggtaaatc cgcagtgcat
     1921 cctcgtgcgc cacaccgaac ttgttgccct tgagaccggt cgagatatag gggtgggttt
     1981 tcgggtcgac attcgggttg acgcgaatgc tgatcggcgc gatgcggccc atggccagcg
     2041 ccaccccgga cagtacatcc agttcggcct cgctctcgac attgaagcag ccgatgcctg
     2101 cggtcagggc ctgctgcatt tcggagcgtg ttttgccgac cccggaaaaa atgactttgc
     2161 gcacatcgcc gcctgccgcc agcacccgct caagctcacc gccggagaca atgtcaaagc
     2221 cacagcctgc acgggcaaac acctgcagca cgcccaagga ggagttggcc ttcatggcat
     2281 aacagatctg cgcattgcgt ccggcaaagc cctgctggta agccgccagg gccgccagca
     2341 tggcagcttt ggaatacacg aacagcggtg tgccgtactg gctggccagg gtatcgaggc
     2401 gggccgcttc gaggtacagg ccgtcctggc ggtaggccag ctggggtgcg cccggcaggg
     2461 cggttgatgc agtggtgctc atgcccggcg ctttcatcgc agcggcaagg tcgcgggacc
     2521 ggatgcgggt ggcggtgccg gcagatcggt cgggacggct gtggcacttt ccggtgcgct
     2581 ggcggcggcg gcgggcgcag gcgccgcgaa aggggcaatg cccgccggac gggctttggg
     2641 cggcggcggg agaaaaaggg ggcccttctg gccgcaagcg gcaagaccca tggcaatggc
     2701 cagggccgcg gcggcggttg aaaggccatg agcgcaggag gcagcctggc ggcggcctag
     2761 aatttgacgc tggatttgaa atcttgcaaa catgatggga ttgtaatgac cgacctggaa
     2821 tatctggacc aagccgaggc cgccctcaag gcgattgaaa tggcgtgtga ccgcatcaac
     2881 gacgaaacgg atgccgacgt cgacaaccag agaaccgggg gcatgatcac gctgactttt
     2941 gccgaccgca gccagatcat catcaatctc cagaagccgc tgcaggaaat ctggatggcc
     3001 gcccgcgctg gcggttttca ttacagattc aaccagggcc actggacaga caccaaggat
     3061 ggcagcgcgc tgtttgctga gctgtcgcgc tacgccagcg cgcaggccgg gcagccgctc
     3121 gtgttcagcg caccgtcagg cgcgcgctcc gctagttctt gaacagatcc agaatccggc
     3181 gacgttcatc gctcggtggc ggagtctgga tggtggcgcc gggggggact ggttggccgg
     3241 tgatcgggtt gtcgaccccg gcgcctgcgg cgggacggtc tcccagtccg acgctgctga
     3301 tgccggaacc tttggcgtat tcatcataaa accactctcc gccgatattg acaacaccct
     3361 cgggggctga aaactcggtc accggaacgc ctttgagcgc gtagtccatg aagttgatcc
     3421 agaccggcag gctcaggccg cccccggtct cccggtcacc gagttttttc ggcgtgtcgt
     3481 agcccatcca tacggctgca gtgagggtcg gctggaagcc cacaaaccag gtgtcgatcg
     3541 aatcgttggt ggtgccggtt tttccataga ggtctggccg tttcagggtc gcctgggcct
     3601 tggcggcggt gccgctgcgg gcaacttcct gcaagaggct ggacatgatg aaggcgttgc
     3661 gcgcttcgat gccacgaacc gactcgtcga gtgttggcac cgggctgtcg accacaacct
     3721 tgcctttctg gtcggtgatt ttggtgatca ggtagggatt gacgcggtag ccgccattgg
     3781 caaacaccga atagccgatg gccatttgca gcggtgtgac cgagccggcg cccagtgcca
     3841 tcgtcaggta ggccgggtgt ttgtcaccgt caaaaccaaa ccgggtcacc cattcctggg
     3901 catatttggg gccgatggcc tgcagtatcc ggatggaaat catgtttttt gacctggcca
     3961 gaccccggcg caggctcatc gggccttcga aggtgccgtc atagtttttt ggctcccaag
     4021 gctggccgcc ggtgacgcct gcgtcaaaaa acaagggcgc gtcgttaacg accgtcgccg
     4081 gggtgaaacc tttctcgagg gcggctgaat agataaacgg cttgaagctg gaaccaggct
     4141 ggcgccaggc ctgcgtcaca tggttgaact tgtttttctc atagtcaaag ccgccgacca
     4201 gcgccctgat ggcgccgttg cgcgggtcca gcgccacaaa agcgccctcg acctccggca
     4261 gttgtgtgat ttcccaagta tttttgggtg ttttggtcac gcggatcagg gcaccgggac
     4321 ggattctgat gttgggtgcc gctttttcgg acaggcccga ctgggccggt ttgaggccgt
     4381 cgccggtgat ttcaatggtt tcgctgttct ggcgaagggc cacgattttt ttcggtccgg
     4441 cctccagcac cacagccgac atcacgtcat cgttgtccgg attctcggcc aatgcgtcgt
     4501 caatcgcgtc ttcggtttcc ttgggatcgc tggacagggc aatgaatttt tcggggccgc
     4561 ggtagatctg ccgcctttca taatccatga tgcctttgcg cagcgccttg taagccatgg
     4621 tctggtcggc agcccgcaag gtggtgaaga cattcaggcc gcgcgtgtag gtctcgtcgc
     4681 catactggct ataaaccagc tggcgcacgg tttctgccac aaattcggca tgcacccgcc
     4741 cagtgtcagg gccggatttg accttgacct cctcgttttt ggccatcacc gcctgttccg
     4801 gcgtgataaa gccgttttct tccatgcgct caatgatgta aagctgacgc gcccgggctc
     4861 gcctggggtt gctgaccggg ttgtaggcgg aaggggcctt cggcagcccc gcgagcatgg
     4921 cggcctcggc aatgctgatg gatttgagag gcttgccgaa ataaatctcg gacgcggcgg
     4981 caaagccata agcgcgttgc cccaggaaga tctggttcat gtagatctcc agaatctggt
     5041 ccttgctcag aaggtgttcc agcttgaagg tcagcaaaat ctcgtagatc ttccgcgtgt
     5101 agctcttttc ggtggacagg tagacgttgc gggcgacctg catggtaata gtcgaggcgc
     5161 cctgactctt ggctcgcccc acattggcga ggccggcccg gatcacaccg atgtaatcaa
     5221 cccccccatg ggagaagaag cgtgcatcct caatggccag cactgcatcc ttcatgacct
     5281 tgggtatctc gcctatcggt gtcaggttgc ggcgctcctc gccgaattca ccaatcagaa
     5341 ccgcatccgc tgaatagacg cgcagaggaa gtttgggtcg gtagtccagc aaatcggaca
     5401 catccggcag gttcgggtag gcgaccgcca gcgcaaccgc aatgatgatc aggagtgaaa
     5461 caaggccagc cagcgccaga cccaagcccc acgcaaaggc cttgagcaac ttgagcggcc
     5521 agggccgctg gctggcaggg gaggggcggg attcggaagt cgaagaattt gacggcatgg
     5581 gtttaacgta agttcgcgcc agcattgtaa aaatccacga tgctggaaga ctttgcaggt
     5641 tgtcagggat ttcacatttt gcaattgtgg tcttgtcgcc gcgctggcat cagcccggtc
     5701 aagccatggg aagcgtctac ttttgacaac ggtgatagta agctaaaacc gaaaaatcct
     5761 ttgttgtaca aggaccttac tgatagcatt caagtgattt cttaagtttg tagggctaca
     5821 tattagccgt catctgggga ccgtcttgat ctctctgggg tctttattaa accgtcaatc
     5881 gccagccgtg ctgggcttgg acatcagttc gtccagcgtc aagctggtag agctcagcca
     5941 ggacaaggcc ggcaacctgg tgcttgaccg gtgtgccatt gagcccctgg aacggggctg
     6001 gatcaccgac ggcaacgtcg agaaatttga cgaagttgcc gaggccgtgc gccgactggt
     6061 caaaaagagc ggaacacgta ccaaaaatgt ggtcatggcc ctgcccgcgt cggcggtgat
     6121 caccaagaaa atcatcctgc ccggcggaat gagcgagcag gagctggaga tccaggtaga
     6181 ggccgaggcc aatcaataca tccccttttc actggatgag gtcagccttg atttttgtgt
     6241 cataggtccc agcgccagct cggcgggtga cgtggaagtg ctgatcgccg catcccgcaa
     6301 ggaaaaagtc aatgaccggc aggggctggc cgaggcggca ggtctcaaac cggtggtggt
     6361 ggatgtggag tcctatgctt cacggttggc gacggcccga ttgattgaaa gcctgccgaa
     6421 caagggcttg gacaccatgg tggccctgtt cgaagtcggc gccctcacca ccagcatgca
     6481 ggtcatcaag aacgacgaag tgctgtatga gcgcgatcag gcttttggcg gggcgcagct
     6541 gacccagctg atcgttcgcc agtatggctt ttctccggaa gaggcggagg gaaaaaaacg
     6601 tagcggcgac ctgccggatg actacgatgc cagcgtgctt aaaccctttg tcgacagcat
     6661 ggctcaggaa attggccgtg cgctccagtt cttttttacc agtacaccgc acaacaaggt
     6721 ggattacgtg atgctggcgg gcggttcgtc cgcattgccg gggttgaccg aggcagtgac
     6781 ccagcagact tcatttgcct gcatggttgc caatcccttt gagggcatgg ttctgggcag
     6841 caatgtaagg gagaacaaga tgaagcgtga agccgcgtct tacctgaccg cttgcgggct
     6901 ggcgttgcgg aggtttttcc agtgattctg gtcaatctgc ttcctcatcg cgaagcggcg
     6961 cgcaaacgcc gccgtgaggc ttttttcgtc gccttgggca ttgctgccct tattggcggt
     7021 ctgctctgcg gagcggtcta ttcctggtac caggcccaaa tttcggggca gcagggcaag
     7081 aatttatttc tgaaaaccga aatcacccgt cttgaaaacc agatcaagga aattgcaggc
     7141 ctgcagcagg aaatcgctgc tttgcgtgcc cggcaaatgg cggtggaaga cctgcagggc
     7201 aaccggaatc tgccggtcta tctgctcgat gagctggtca ggcaactgcc ggacggtgtt
     7261 tacatcacca gcatgaaaca ggacaaccag gtggtgttgt tgactggcgt ggcccagtcc
     7321 aacgagcggg tctccgaatt gctgcgcaat ctgggcaaca acagtccctg gttgacccgg
     7381 cccgaactga ttgaaatcac aggcagaaca atcacgctgg gcgcacgcga ccagcggcgt
     7441 gttgcgaatt tttcgatgcg cgtgggactc aggcgggcca cggatgtgca gaaagcggca
     7501 gcatcagcgg cggggtcttc cggcgccgcc gtgggcaagc ccgcccagcc aggcactcct
     7561 gcggccaaga gctgacgaac cagccccgaa caggcactga ctatggcacg tacaccgaaa
     7621 ttcaatgtta atttttctgc gcttcaggac ggtctgaagg cgcaatttgt cggtctcaac
     7681 ccgaatgacc cttcttcctg gccggcgttt cctcgttttc tcctgtatgt gtttgtgacc
     7741 gccgccgttg tggttgcgct gtggtttgta tggctcagcg attctgacga ggaactcaag
     7801 gcagaacagg ccaaggaagt acagttgcgg gaagatttca agaagaagct ggcgcaggcg
     7861 gtcaacctgg aagccctgaa aaagcagcgt gagcaggtcc agcaatacgt gacccaattg
     7921 gagaaacaac tgcctagcaa ggccgaaatg gatgccttgc tgtcagacat caaccaggct
     7981 ggcttgggcc ggagtctgca gtttgagctg tttcggcccg gacaggtcag cgtcagggag
     8041 tattacgcgg agctgcccat tgcgatcaag gtgtctgggc gttatcacga catcggggct
     8101 tttgcagccg atattgccaa tctgtcccgc attgtgacgc tcaacaacat gacgattgct
     8161 ccggtcaagg acggaatgat gagcatggac acgaccgcca agacattccg ctatctcgat
     8221 cctgaagaag ttgccttgca gcgtaaagcg gcgccagcga aaggccccaa gaaatgatgc
     8281 gaacaactat ccaaaagacg ctcatgctgg tgtccacggc ggtggtttgc atgaatctga
     8341 cggcctgcag ttcttccgag caagaggaat tgcagcagtg gatgaccgag caacgcaatc
     8401 tgacgaggcc caggatcgac cccttgcctg agcccaccag gttttcgccg caggcttata
     8461 cccaggaggg tgctgtcgag cctttcagcc cccagaaatt gacgcaggcg ctgaaccgag
     8521 actccaaccg ggcaactgcc aatgcagccc tgattgcgcc tgagttgagt cgacgcaagg
     8581 agccgctgga agcgatgccg ctcgatgcgg tcgcgatggt gggtagcctg accaagcaag
     8641 ggcagccggt ggcgctggtg cgggtggaca acctgctgta ccaggtgcgg gtgggcaact
     8701 acttgggcca gaactacggg cgcatcacga aaatttctga gactgaggtc gccctgcggg
     8761 aaattgtgca ggacgctgca ggcgaatggt tagagcggac agcgacttta cagcttcaag
     8821 aggggtcaaa atgaacaaaa atgcatggac gacgcaggcc gtcccgacgc ggggtgcatg
     8881 gtcgcggata tcggctggcc tggccatcgg tacggcttgg gtgacgctcg cgctggcaac
     8941 gcaagcatgg gcccagacgc ccaatgccat cgaggcggtg tcgggttccg tccagggtgg
     9001 ctcggaagtc attcgtatcg acctgaaaga agcgctgacc gcagttccca ctggctttac
     9061 gattcaaacg cccgcgcgga ttgcgctcga ttttcctggc gtcagcaatt ccatgggccg
     9121 ctcggccctt gaattgaacc tgggcaatct gcgctctgcc aacgtggtcc aggcgggcga
     9181 aagaacgcgc gttgtcctca atctgaaggc gtcgacggcc tacacggcgc agattcaggg
     9241 gaaatccttg ctgatcacgc tggacccggt ggccgttgcg acgccctcac ccgtcgttgc
     9301 acctgtgttt gccgaaaacc gcaaccgcga caccttgccg ctgaaagaca tcgatttccg
     9361 gcgggggacg gacgcttcgg gccggattgt ggtcgatctt gccaataacc aggtgggcgt
     9421 cgacatccgc cagcaggggc agaccctggt ggtggaattc ctgaaaaccg ccttgcccga
     9481 gggcttgcgt cgccggctgg atgtgtccga cttcggcacg ccggttcaaa gcgtgaccac
     9541 ttcccagctg ggggacaagg tccgcatggt ggtcgaaccc aaaggctcat gggaacacag
     9601 tgcttaccag agcgacaacc agtttgtgct ggaagtccgt cagcaaaaag tggacgaatc
     9661 caagctgtcg caggggcccg gctacacggg tgaaaaactg tccctgaact tccagaatat
     9721 cgagacgcgg tccctgttgc aggtcattgc cgatttcacc aatttcaata tcgtcacatc
     9781 cgacagcgtg tcaggtgccg tgaccctgcg cctcaaggat gtgccatggg atcaggctct
     9841 ggacatcatt ttgcaggcca aaggtctggg aatgcggaaa acaggcaatg tgttgctgat
     9901 tgcacccaag gacgaactgg ccgcaaaaga caagctcgat ctggagtcgc gcaacgcgat
     9961 ccagaccctg gagtcggtac gcacgcagtc cttccagatc aactatgcaa aagcagcgga
    10021 tatcggtgcc caaatcacgg cgggtggcac cggcccaacc agtgcccgca tcctgtccaa
    10081 tcgtggcagt gcaattgcag aggccagaac gaatcagctg ttcgtgaccg acattccctc
    10141 caagctggaa caagtgcaga gcttcattgc aaaaatagac
//
LOCUS       DAIDFG010000087        10154 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_87_length_10154_cov_17.091890, whole genome shotgun sequence.
ACCESSION   DAIDFG010000087 DAIDFG010000000
VERSION     DAIDFG010000087.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 10154)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10154)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10154
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87_length_10154_cov_17.091890"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1763 a         3363 c         3251 g         1777 t
ORIGIN      
        1 gtgtggttgt ggccgacatg ggccactaca gccggttcat catggacacg ctgctcaaac
       61 accccagcgt gcaggactgc aagaccagtt ttgtgctgga ccgcgtgaag tcgaccacgg
      121 cggttccggt ttgatgcgct ggcctgcacg gtgggcctgg tcaggggctg atttaggggc
      181 cggccaccct tttgatgtcg attaatgccg gttcggcagg tgttttttga ggaaatttgc
      241 tacgatattc atagctgtca gcgcccatcc aataagggca aatggctatt ttttgctaaa
      301 aacccgagcg tctccccggg gccgtgcgat taaatagcct tcagggctgg acattctagg
      361 gaaaaccctt atattgaact tatggttacc cccggcacat tcatcaaagc gtccatttct
      421 gcgggcaagg agttcttccg cccgccggtg gacgacgcag cgcgcgcgcc gcacaggccg
      481 cctgtgctgg tgcccatccg ctccatcggt cccacccacg gcgaacgcat cgaaaaacat
      541 ttgctggcgc tggagccgca tgaccgttac ctgcgttttg gctacgccgc ccgcgacgaa
      601 cagatccagc gttacgtggc cggcctgaac ttcgagcgcg acgagatttt cggtatctac
      661 aaccgcaagc tcgaactgat cgccatggcg cacctggcgc tgtcgatcga cccggagctg
      721 aaaagctgcg ccgagtttgg tgtgtcggtg ctcaagcacg cacgcgggcg cggctacggc
      781 gcgcgcctgt tcgaccgcgc cgtgatgcat tcacgcaatg agggggtgag catgatgttc
      841 atccacgcgc tcagcgaaaa caccgccatg ctgaacatcg cccgcaaggc cggcgccgtg
      901 atcgagcgcg acggttcgga aagcgaggcc tacctcaagc tgcccccggc cgacctggac
      961 tcgcgcatga ccgagatcgt cgaggagcag attgcccaga ccgactaccg cctgaaagtc
     1021 caggccaaac agttctggaa catcctggcc atggtccagg aagtgcggca gggcgtgcgc
     1081 gacagccgcg accgcatgcc tatataaaag cccccacggt cgctcgcttc gcgtagctcc
     1141 ctgcccccga gggagcccct gcttgaatgg ggatggcccc agaccgaaaa gtcaatctgc
     1201 cgcgggtttg tagcgccgcg cgctgctccc atggcgccgg tcggccatcg gcaggccgtt
     1261 ctggccggcc atgacgtcag cgcgttgctg caggcgttcc agcatctcgc caagttgagc
     1321 cgtttcctgg ggcgtcaggg ccgcaagcac gtcgcggttg atccggctga ccaagggaaa
     1381 caactcactg tgcagggcaa tgccggctgg cgtgagcgtc agctggatgt gccgcgcgtc
     1441 cccggcgcgg ctggtgcggc tgatcagctt tttgccggcc agcgtcgtca ctgctttgga
     1501 ggtgcgcggg cggtccaggt gggcctgctc cgccagcgcg gacgagccca tgggcccgcg
     1561 cgcagccagc acggccagga cgcgccactc gcgccgggtg atgccaaatt gaccttcgca
     1621 gtaacgcacc acgacacccc cagcggccgc ctgcaggcga ctcagccggt acagcaggag
     1681 gtcgtcgaga gtttctggct gcgccagacg cggcacggca ggaagggagt gaggcatcag
     1741 ggttattacg ggcgatgatt gatttccgca atcaatggac gtgccgggaa aatcgcggct
     1801 ccaaaccgga gaaatcatca tgccacagcg cctcgagctc gcaccacctg ccttcaaccg
     1861 ccgcaaaatc ctgattcaca ctgccgccgc gggagcgctc gccgccggcg ttggcgtgcc
     1921 gcgctgggcg cgtgcccaaa gcgggccgat gcgcattgtg gtgggctatc cgccgggcgg
     1981 cggtaccgac cgcgcggccc ggctggtggc cgaccgcctg cagcagttgc tcaaacgcac
     2041 ggtgatcgtg gagaaccgcg tcggcgccgg tggacggctg gctgcacagc agctggtcaa
     2101 tacgccgccg gaccagacgg tgttgctgct ggccaaccct gccgtgatga gcgtgtcgcc
     2161 cctgatctac aaagacctca aatatgacgt gcaaaaagac tatgtaccgg tcgccgccgt
     2221 cacggattac gatttcggcg tggcggtcgg gtccgccctg ccggtcaaag aagtgcagca
     2281 catgctggcg tggattcgcg ccaacccgac caaggccaat gccggcgtgc ccggcaccgg
     2341 cagcctgccg cattttttct cgctgatgct cagctacatg gcgcaggtaa acatcgaggt
     2401 ggtgggttac cgggggtccg cgccgctgat caccgacctg atcggcgggc agatccccct
     2461 ggcatttgac acgctggact ccctgctacc gcagcatgaa ggcggcaagc tgcgcatcct
     2521 ggccacctcg ggcctcaagc gttcgccgtt ttcgcccacg gtgcctacct tcaaggagca
     2581 gggcctgccg ctcagtgccg tgggctggaa cacgtttttt gccccatcca ccacgccccg
     2641 ggccacggtg gagttgctga gcaaggcggt gttccagatc atgtccgagc ccgaaacgcg
     2701 cgccaaattc actgccgcca aaatggtgcc ggtggccatg acccaggcgc agaccgaggc
     2761 gtcgttcaag gctttcaaca cacagtggac gccggtgatc cggcgatccg gcttcaagcc
     2821 cgagtcctga gcgggtgaac acagctttga ccgcctcgcc acgccccaac atcgtcttca
     2881 tcgtcgcgga cgacctcggt tatgccgacc tcggctgcta cgggggccgg gcgcccgtct
     2941 cgcccgtgct cgacagcctg gccgcggggg gcatgaagtt cacccagggg tattccaact
     3001 ccccggtgtg ttcgcccacc cgctttgccc tgatgaccgc gcgttaccag taccgcctgc
     3061 gtggcgccgc cgaagagccg atcaacaaca aaagccgcgg cagcaccacg ctgggcctgc
     3121 cgcccgaaca ccccaccttg ccctcgctgc tcaaggcaag tggctaccgc acagcgctga
     3181 ttggcaagtg gcatcttggc tatccgccgt ctttcggccc cttgcgttcg ggttatgaag
     3241 agttctttgg cccgatgtcc ggcggggtgg actacttcac gcattgcagt tcgaacggca
     3301 gccacgacct gtggttcggc gaagaggaga aaaaggaaga cggctacctg accgacctga
     3361 tctcgcagcg ctcggtggac tatgtcgagc gtatggccgg cgctgccaaa gccggcacgc
     3421 ccttttttct gagcatgcat tacaccgcac cgcactggcc ctgggagacc cgtgacgacg
     3481 aggcgctggc gcaggacatc agggacaacc tgtttcacct gcacggcggc aacattcaca
     3541 gctaccgccg catgatccac cacatggatg aaggcatcgg tcaggtgatg gacgccctgc
     3601 gccggcacgg cctggccgac aacacgctgg tggtgttcac gagtgacaac ggcggcgaac
     3661 gtttctccga cagctggccg ctggtgggtg gcaagatgga cctcacggag ggtggcattc
     3721 gcgtgccgtg gatcgcccac tggccggcgc agattgcccc cggccaggtc agcgagcagc
     3781 actgcatgag catggactgg tccgccacct tgctggccgc cgccggcgtg gcggccgatc
     3841 ccgattaccc gctggacggc gtttcgctgc tgccggtgct gcagcagccc ggccggcgct
     3901 ttcaccggcc gctttactgg cgcatgaacc accggggcca gcgggcgctg cgcgacggcg
     3961 actggaagta cctgcgggtg gacggcaacg actacctctt caacatcccc gccgacgaac
     4021 gtgagcgagc caacctggcc ggccgcgaag tcgggcgcct ggcggcgatg cgcgaggcat
     4081 gggaccgctg gaacgacgac atgccgccca ttccggcaga tgccacggta agcctgggtt
     4141 attcggtcaa ggacatgccg cagcgctaga tatgccgacc cccacggtcg cgcacttcgt
     4201 gtcgctccct gcccccgagg gggccgctgc gcctgcgggc cggcaaagcc ggccccgcgg
     4261 cccctgcttg acttaagagc ccgaaccgtc gctcacttct tatcccatcg tcctgagcct
     4321 gtcgaaagat gcccccgagc ggctgctgcg cctgatcgaa gtacgttatc cttggcaact
     4381 tgccaactac cgcacacctc cgtgtccgac ttccccccca gtagaccgcc caagtccgaa
     4441 gacaagcgcg gcttcctcca gaagctggcg gaaatgcttc accccgggcc cgattccaaa
     4501 gacgagctga ttgaaaccct ggtcgaggcg caggacaacg atgtcatcgg cgcgcaaagc
     4561 cgggagatgc tcgaaggcgt gatccgcatg gccgacatga cggccggcga cgtgatggtg
     4621 gcaacgcccc gcatggacgt catcaacatc gacgccccct tcgacgagtt gctgcacctg
     4681 gtgatcgaca ccgcgcactc gcgttttccc gtttacgaag acgaaaaaga aaacatcatt
     4741 ggcatcctga tggccaagga tctgctcaag ctgcagcgtg cgcccgagct caacatccgc
     4801 gcgctgctgc gcccggcggt atttgtgccc gagagcaagg gcctgaacga cctgctgcgc
     4861 gaattccgcg gcaaccgcaa ccacctggcc atcgtcatcg acgagttcgg ccgtgtcgct
     4921 ggcctcatca cgattgaaga cgtactcgag cagatcgtcg gcgagatcga ggacgagttc
     4981 gacatcgccg aggacgaggg cgacatcttc ggcctgaccg accacagcta ccgcgtcagc
     5041 ggggacacct cgatcgagcg ggtcaacgag gccttcggcg tgctgctgcc cgaaaccgac
     5101 ttcgaaacca taggcggcct ggtcgcccac gagatgggcc atgtgcccaa acgcggcgaa
     5161 ctctacgcgc tggccggcct gcagttcacc gtgctgcaca ccaaaggggg cgccgtgcgc
     5221 tggttcaagg tgtctcccgt cgccacgggc acctgaggcg cagcggacaa aaagcagcgg
     5281 atggcacgga attcgatcca cagcatgatg tcggcgctgc ccacgttgcc ggccctgctg
     5341 gagcatatcg ggcacccgga gccgcgcaag gggctttcct ttcttgatgt tgctattgtt
     5401 ttaatagctg gatgcgccca tgccttgagc gttgcgtggc ctttttcctc cgggttggcg
     5461 cagggccagc cggtctggtg gctgcaactg ctggccctgg ctgcgcttgc ccggtcgctg
     5521 gaccgctgca cccgcttcaa gcaggggctg ctgctgggct ggctgtttgg cagcgcctgg
     5581 ctatgcggta tcttctggtg gctgttcatc tcgctgcatg tgtacggcgg gctggccgcg
     5641 ccgctgacgg tgctggcagt ggtgggtctg gcgacttttc tggccaccta ttacgcggtg
     5701 gcctgcgcgg cctatgtcac cctggcccct gccggacgaa cacgccgggc gattgttttt
     5761 gcggcactct ggctgctggc cgagctggcc cgggtggagt ttttcaccgg ctttccgtgg
     5821 ggcgcggccg gctacgcgca cctggacggc tggatggacg gatatgcgcg gtggatcggg
     5881 gtgcacggca tcaccttcct ggcggctttt ttggccgcca gcctggccgg cctgggccgg
     5941 tcacggcgcc agctggtcat gttcccggta ctggcggcgc tgatcgtggc aggggcgctg
     6001 gcggcgggaa agcagacctc caccccggcc ggcagcctgc aggtgacgct gctgcagggc
     6061 aacattgccc aggacgaaaa attccagccc gggaccggcc tgccgacggc gctgcgctgg
     6121 tacggcgagc aactgcaggg cgccaaaacc gcgttggtgg tggcgccgga aacttccctt
     6181 ccgctgctgc cgcagcaact gcccgaaggt tactggagcg ccgtgcaggc gcgttttgcc
     6241 tcggggcagc aggcggcgct gatcggcatt ccgctgggca gctacagcgc gggttacgcc
     6301 aattcggcgg tggggctcaa gcccggccag gcccaggctt tccgtttcga caagcaccac
     6361 ctggtcccgt ttggcgagtt cgtcccaccg ggttttcgct ggttcatcaa cctcatgaac
     6421 atcccgctcg gcgacttcaa gcgcggtccg ctgggccagc cttcgtttga ctggcagggc
     6481 cagcggctgg cgcccaacat ctgctacgaa gacctgtttg gagaagagct gggcgccagt
     6541 ttcaccaacg ttgccacggc acccacggtg ctcgtcaacc tcagcaacat aggctggttc
     6601 ggcaacacga ttgccatcga ccagcacctg gccatctcgc gcatgcgggc gctggagttc
     6661 gagcggccca tgctgcgcgc caccaacacc ggcgccacgg tcatcatcga ccaccgcggc
     6721 aaggtcaccc acgaactgcc gcgccacacg cgcggcgcac tggtgggcga ggtggaaggc
     6781 cgcaacggca tcacgcctta cgcctggtgg gtgtcgcgtt acggcctgtg gccatggtgg
     6841 gcgctgggcc tgggtgtcgc gggactggcg ctggcttttc gcttgcgttc aggccgcttg
     6901 ctggcctgaa cgcacgagcc ggcccggccg tgcggccgtg acctcacctt cgcgggccac
     6961 acactggccc gcgacccagg tgccgatgta accctgcgcc ttttgcagaa aacgcttgcc
     7021 gccggcgggc aggtcgcgta ccagctgcgg caggccgacg cttaatcgcg cggggtcgat
     7081 gacgttgatg tcggcacgca ggccgaccgc cagccgcccg cggtcgctca gccccaggta
     7141 acgcgcattg cgcgagctca tcatttccac cgcctgttcc agcggcaggc ggtcctgcgc
     7201 gcggccctgc acccagtgcg tgagcatgaa agtggaaaaa ctcgcgtcgc agacggtgcc
     7261 cacatgtgcg ccggcgtcgc ccaggccgaa cagcgcgcgc gggtggccca gcatctggcg
     7321 cacggtgtcg agtgaaccct ggttgtagtt gaagatcgga aagtacacca ggttgccgcc
     7381 gtcgccttcg ctgaagtagt cgtacagcgc ctccagcgcg gtgatgcccc gctgtttggc
     7441 gcggaccaga aagctctgca tcacgttggg ttcatagttg agcgtggcgt ccagcgggaa
     7501 catgcgtccg gcgatcattt caatacgcgc gagcagcatg tccaccagcg gcggaatcgg
     7561 cgtgccgtcg cccgccaggc gctccgactt ctcgcccagt atgcgggctt ttctgtctgg
     7621 tgcccgcatc gctgctgcgc ggtccgctac agaaagcgta gcaatttcct tgtaccccgg
     7681 aaagcccatg aaagggtgaa aactcgcgtc cagcccgttg atgacgccga tgccgcgcgc
     7741 cgcggtctgc aggtacagcg gcaggccgcg cgccacggcc gcttccacgc gggcctgcat
     7801 ggccaggtac tgctcgccgc ccgggtcgcg ctgcagccag ctcatggaca gcggccgccc
     7861 gctggccgcc gccagctgct cgaccaggtc gaactcggca tcgaagttct gcggcccctg
     7921 catcaggtcg aaatcgctga ccacctgcac cacgccgtgg ctcaggccgt caaaggcgcg
     7981 ggcaatgccg gtcagctcgg ccgcgctggc gatcgaggcc ggcgtgtcat gcccctcggc
     8041 cgtgcggtgg ttgtccgaac ggccggtgga aaagcccacc gccccggccg ccagtgccgt
     8101 gcgcaggtgc tcgcgcatgg cgccaatgtc ctgcgcgctg gcggcttcgc gcgcctcggc
     8161 gcgttcgccc atcaccagca tgcgcagcgg gtcgtgcggc accatggtca tgaagtccag
     8221 gctgtgtggc atggcatcga gcgcgttcat gtagtcgcca aaactctccc atgaaaattt
     8281 cacgccttcg gtcagggcca cgccggggat gtcttccacg ccctccatca gcttgatcag
     8341 gcggtcctcg ctgccgggcc gcagcggcgc gaagccgacg ccgcagttgc ccatgaccac
     8401 ggtcgtcacg ccgtggtgga tgctggggct gaaggtctcg tcccaggtgg cctgtccgtc
     8461 gtagtgggtg tggatgtcca caaaaccggg catgacccag gcgccgctgg cgtccacggt
     8521 gtcgcgcgcg gccgccgtga tgcggccgat ctccacgata cggccgtcct gcacgccgac
     8581 gtcggccaca aacggcgcgg cgccgctgcc gtcgaccacg gtgccgcctt taaaaagcaa
     8641 gtcgagcatc tttttttctc cagaagtaag ccagcgacag gccgctgacg atgtgccaca
     8701 tgccccacag gctggcgatg atgaccatgc cgaggtccga gttgaactgc acggcaatga
     8761 tgcccagggc caggcccgag ttctgcatgc cgccctcgat catcacggcg cggcggtcgc
     8821 gttcgctcac gcccatggcc ttgctggtga cccagccgaa aaacaggccg ctggcattgt
     8881 gcgcgatcac gatgcccagc atgggcagca ggcccagcgt cagcagctgg cgctccttga
     8941 tcagccccag cacgatgaaa gccagcagtg ccagcagcga aaagttgccc agtggtttgc
     9001 ggatgcgcgc cgtcagcgcc ggcaactggt gcgagaacag caggcccagc gccatgggca
     9061 gggccagcag cagaaagagg ctgatccaga tgcccgaggg gtcgatgtcc agtgtcttga
     9121 gccagccggc cgtggccggg ttggccgcca ccatccagct gaagttgaac ggtgtggcaa
     9181 ccagcgcaat gatgctggcc acggccgaca cgctgaccga cagcgccgtg ttgccgcggc
     9241 ccaggtgggt gaccacgttg ctcaggctgc cgccgggaca ggccgccacc aggatcatcg
     9301 cggcctcgac gttgggcggc aggtccagcg ccagggtagc cagccaggtg cccacgggca
     9361 gcaggatgaa ctgcgggatc agcccgcaga ccaccgcttt gggcgtttgc gcgacgcgcc
     9421 tgaagtcgct caccttgagt tccagcgcga ccgaaagcac catggtggcc agcaccaggc
     9481 tcaaaatcaa ttgttgggtt gtcatgacac tttccttgcg tctgagggta acccggtgaa
     9541 agctccgtaa aatcaagggt tttcgcgggt tcaggcccgc tttgtctttc ccatccggcc
     9601 ctgcgtcgcc gcacacccta tttatgttga cctttcagca aatcatcttg aagctccagt
     9661 cctactggga tgcccagggc tgcgccttgt tgcagcccta tgacatggaa gtgggcgccg
     9721 gaacgtcgca cactgctacg tttttaagag cgctgggtcc ggagccctgg aaggccgcct
     9781 acgtgcagcc gagccgccgc ccgaaagacg gccgttacgg cgagaatccg aaccgcctgc
     9841 agcattacta ccagtaccag gtcgtgctca agcccgcgcc cagcaacatc ctggagcttt
     9901 acctcggctc gctcgaggcg ctggggtttg acctgaagaa aaacgacatc cgcttcgtgg
     9961 aagacgactg ggagaacccg acgctgggcg cctggggcct gggctgggag gtctggctca
    10021 acggcatgga agtgacgcag ttcacctact tccagcaggt cggcggcatc gactgcaagc
    10081 ccatcaccgg cgagatcacc tacggcctgg agcgcctggc catgtacctg cagggcgtgg
    10141 acaacgtcta caac
//
LOCUS       DAIDFG010000088         9941 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_88_length_9941_cov_18.955088, whole genome shotgun sequence.
ACCESSION   DAIDFG010000088 DAIDFG010000000
VERSION     DAIDFG010000088.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 9941)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9941)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9941
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88_length_9941_cov_18.955088"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1815 a         2991 c         3112 g         2023 t
ORIGIN      
        1 gtggaggcct cggatacttt cgggaaatca ttgggtgttc gactaggtgg tcggccgttt
       61 acgctcaacg gtgcaagtaa tgcatcattc ggtccgactt acatcaatcc aggtaccaca
      121 cccggagctc ccgttcaggg cgtgaactac ggaactacta caggcgactt tctgaacctt
      181 cctggcgtgg gccaaaacgg attcaatgct gccacttttg cagtttccct cttttcgtca
      241 ggcgccactc gactgctcaa tcttgaaatt tcagccatgg aagccgatgg caaaggcaag
      301 attgtgtcga gcccgcgtat cgtgacggcc gaccaggtca aggccctgat cgagcaaggc
      361 acggagttgc cgtaccagca agcgacttcc agtggcgcga cctccatcgc cttccgcaag
      421 gccaacctga agctggaagt gaccccgcaa atcacgcctg aaggaaacat catcctgaat
      481 gttgacgtga acaaggacag tgtgggccgg tcgacgagca acggctttgc gatcgatacc
      541 aaacacattc agacccaggt gttggttgaa aacggcggca cagtggtgat cggcggcatt
      601 tttgaacaga acgagcgtga ggacgtaacc aaggttccct tgctgggcga tctgcctgca
      661 gtgggcaacc tgtttaaaac caggacacgt acggccaaca agtccgaatt gctggtcttc
      721 atctcgccca aaatgatttc caacggcgcg gccctgcgct agcattgact acgcgacgcg
      781 acgcgacgcg aagccgggat ggcctcccgg ctcgccttgc cgggctggct gttctccgag
      841 ggatacggtg tctgcatgtc ctgttcaggc cgagatatgt tcaacgacac gttctttttt
      901 tgttgccgtg attcatcctg acgggggcat ctgttgagcg agcatgccca cgtggcgctg
      961 atcggtcttc ccggatccgg caagtccacc atcgggcgcc aactggcccg acgtctcggc
     1021 cgacctttca tcgacaccga ccaggtcatt gagcagcgcg tcggtttgtc catccgcgag
     1081 ttttttgagc gtgagggcga ggagtccttt cgtgaccttg aacaaagcgt catcgatgaa
     1141 ttgacgctgg gccagccctg cgttctatcg acgggtggcg gggcggtgtt gcggccggcc
     1201 aatcgccagc atttgaagca gcgcacccag gcggtgtatc tgcattcggc acccgaagag
     1261 gtctttcgcc gcttgcggca cgaccggaat cgcccgctgt tgcaggtgcc tgacccgctg
     1321 gcgcgcttgc gcgagctgta tgcgctgcgc gacccgctgt accgcgaaag cgcccgattt
     1381 gtggtggaaa ccgggcggcc ttcggtggct gcactggtca acatggtgat gatgcagctt
     1441 gagctggcgg gtgttcttcc tgcgcctggc gcctgacgcc ccatctgccg cagacgccag
     1501 gctgatgtgc agcacggtat gacggcgaag cgccgtgatg cgggccgcag agcgccgtac
     1561 ggaccggcag agcctctaca cttgtgtcat ggcttcaaat tccctcctga caacgcagca
     1621 ggttttcatt gacctgggcg agcgcagcta ccccatcacc attggtgccg gcctgctggg
     1681 tgatcctggt acttacctga gtctgccgag tgcaagcacc gcactgattg tgtcgaatga
     1741 cacggtggcc cctctttatg cccagtccct gcagctggca ctgcaggggc attacaaaaa
     1801 ggtgttgcag gtgattcttc ccgacgggga ggcacacaag gactggccca ccctgcaact
     1861 gattttcgac gccctgctgg ccaacggctg tgatcgcaag accgtgcttt ttgcgctggg
     1921 cggtggcgtg gtgggtgaca tgacgggatt tgcggcagct tgctatatgc gcggtgtgcc
     1981 gtttgtgcag gtacccacca ccttgctggc gcaggtggat tcttcggttg gtgggaaaac
     2041 ggccatcaat cacccgctgg gcaaaaacat ggtgggcgcg ttttaccagc cggagcgcgt
     2101 gatctgcgac ctggacgtgc tccagacctt gcccccgcgc gagttgagcg ccgggcttgc
     2161 ggagatcatc aagtacggac cgattgccga catggctttt ctggactgga tcgaggcgaa
     2221 catcggggac ctgctcgcgc gcgaaccggc ggcgctggca cacgccgtcc ggcgcagctg
     2281 cgagatcaag gccgcggtgg tgggtcagga tgaacgtgaa gccgggctgc gcgccattct
     2341 gaattacggg cataccttcg gacatgcgat cgagtcggga ctgggttacg gtcaatggct
     2401 gcatggcgaa ggcgtgggtt gcggcatggt gatggctgcg cggctgtccc aaaggctggg
     2461 cctcgtcgac gtggcgtttg tcgaccgcct gcgtgcgctc gtccagcgcg ccgggctgcc
     2521 ggttgtcggc cccaggctgt ctgccaccga caacgcgggg cgctacctcg aactgatgcg
     2581 tgtggataaa aaggcggaag ctggcgaaat cagatttgtc gtgatcaacg ctcctggcca
     2641 tgcaacggtg cgcagtgcgc cggatgcact ggtgcgcgag gtgatcgacg catgctgcga
     2701 atgaatgctc ctgcgatgga ttgctatcaa aataatagct atttgcgcac gtattgaaag
     2761 ggcggaagcc cgattggatg cttgattgat gactgtactg gctccctacg cctgcgaccc
     2821 ggtccggtcg cgcggccgtc gctatcccga gcccgctgcg ccgacccgtt cggcctttca
     2881 gcgcgaccgc gaccggattg tgcattccac agcatttcgc cgcctggttt acaagaccca
     2941 ggtgtttttg aaccatgaag gcgatttgtt ccgcacccgc ctgacacatt cgctcgaagt
     3001 cgcacagctt ggccgttcca tcgcccgcac gctgcagttg aacgaagacc ttgtcgaagc
     3061 catcgcgctg gcacacgatc tcggccacac gccgtttggc cacgccggtc aggatgcgct
     3121 caacatctgc ctgaaaagcc acaaccctga cagcgccggc ttcgagcaca acctgcaaag
     3181 cctgcgtgtg gtggacacgc tcgaggagcg ttacccggac tacgacggcc tgaacctgtc
     3241 ctttgaaacc cgcgagggca ttctcaagca ttgctcccgc cgcaacgccc ggcggatcga
     3301 agggcgtgaa cctggtggcg tggcgcagcg tttcctggcc ccctccggcg acggcccgca
     3361 cgcgcagcag cccagcctcg aggcccagct tgccaatctc gccgatgaga tcgcctacaa
     3421 cgcccatgac attgacgacg gcgtgcgctc tggcctgctg agcctggcgc agctcaagga
     3481 ggtcgagttg tttgagacgt accaccggga aacgctcgcc aggcaccccg gtttgcaggg
     3541 gcggcgattg ttgtttgaaa ccatacgccg catgctcagt gcccaagtgt acgacgtgat
     3601 ggacgccacc ggcgctgcct tgcgggctgc gatgccggcc gatgtgcagg ccgtgcgccg
     3661 gcatccgccg ctggtctgct tcagtgatga aatggccctg aagtcctcgt ccctcaagcg
     3721 tttcctgctg caaaatttat accgccatcc acaggtggtc gagaccacac agacggccca
     3781 gcaggtggtg cgcgacctgt ttgccgctta catggccgat ccggcgcaga tgccgcaggc
     3841 gcacagcgac cgcttcgatc aactgggcag caggcccgtc agcggcacag agcgccaagc
     3901 caaacccgag cgtgtggtgg ccgactacat cgccggcatg acagaccggt ttgccaccaa
     3961 ggaacacgag cgcctgaacg gccgcgtggt gtttcctgtt tgaaagccgt tcatcccgcc
     4021 agcctggtga tcgccgccgg cgtggtggcg gctctgcatg tgggcaaact gccgccggcg
     4081 ttgccagcgc tgcagcaggt gctaggcgtg tccctggtgc aggcgggttt tttgttggcg
     4141 ctgtttcagc tcgccggaat gacactgggt ttgccggccg ggttgctgat tcagcgctac
     4201 ggtccgcggc gcagcatgct cgccggcttg ctggtgctca gcgtggccag cgccctgggc
     4261 ggcgcggcgc agggtacggc ctggctgctt gcaagccgcg tctttgaagg gctggggttt
     4321 ctgtgtgtgg ttttgcccgc gccggggttg ctgcgtgcgc tggtgccctc cgggcgtttg
     4381 agtcgtgcgt tgggtgcgtg gggggcctac atgccgctgg gcgcagcact ggccatgctc
     4441 gtggggccgt gggtgatggc gcaggccgat gtcgcctggg gatggcgaat tttatggtgg
     4501 ctcctgtccg ccttgtccgt tttgctcgcc atcctgttgt atcgcagcgt ccctgctgac
     4561 cagtctctgg aagagaagtc atcgcgagcc tcatcccctg ggacgtcgct tttttccggt
     4621 agccgtggcc ttctgcgcct gacgctgcgc tcacgcgggg tctggctggt ggccctgtgc
     4681 ttttccatgt attcgggtca gtggctggct gttgtcggat ttttgccgtc gatttacgcc
     4741 caggccggat tgccccccgg cgtcgctgcc tggctcaccg cactggcggc ggccgtcaac
     4801 atcatcggca acttgggggc tggccgcttg ctgcacatgc gtgtccctcc gctggccttg
     4861 cttgttgcag ggtttgccgc catgggcgga ggtgccttgt tggcctttgg ggtggatgcc
     4921 agcccttggc tacggtatct ggcggtgctg gctttttcaa tggtgggagg cttgatcccg
     4981 gggacgctgt tcagccttgc ggttcgtctg gcaccgagca cggccaccgt gcccaccacc
     5041 gtgggctgga tgcagcaatg gtcggccttt ggccagttct gcggaccgcc gctggtggcg
     5101 tgggtggctg gtctggtggg acattggcag ctgacctgga cggtgaccgg tgcctgcgcg
     5161 ctggctggca tggggctggc ctggcagatt cacggcctgc tggagcgcga gccctaggtt
     5221 cgcaccgaca tcgactcccg ggtcggcagt gcgaaagtgg ccatgccatg gccttgtcgg
     5281 gtttacccat ggacggagac tcgcccttgc atagccttat gacgtgttgg cattttccac
     5341 accgcctttt ttcgtcccga acgactactc tgcaggactt gtttaatttg ttacgaagag
     5401 cgcgcctgga ctgtttgccc gcgttcaggc atccatcgtg cttaacgaga tgacatttca
     5461 aaaggtattg catgagaatc aatcgacgtg cgctcgtgat gggcggcctg gcagctggcg
     5521 tggcgacgct taccccgctg gcgcattcac agacgggcaa gggtggccgc aaactggtca
     5581 ttggccaatc ggttcctttg acgggtgcgg ccgatcagat cggactggcc tacctcaatg
     5641 gtgcgaagct ctactttgat gcgatcaacg ccaaaaacgg tgcggcgggc tacaggatcg
     5701 aagtcaagac gctggatgat ggctacaacc cggccaaggc ggcggccaac gccagacagt
     5761 tgattgacga aggcgtggat gcactgttcg gttttgtggg aacggccagt tgtgatgctg
     5821 cttttgccgt cgccaagccg gccaacacgc tgttttttgc cccgttcgcc gcatcggatg
     5881 gcctgcgcga agccgcgctg agcaatgcat ttcatatccg tccggccctg gctgacgagg
     5941 cttacaaaat ggtgcgccat tgcgctaccc tgagccagga ccgcatcgct gtttttgcag
     6001 aagacgacgc catgggccgt gccgggctgg cagcggtgaa ccaggcgctg gttgatctga
     6061 agcgtccgcc tcttgtggcg agcgccttcg cgccggtcaa cagcgacaag gtcgaagcgg
     6121 cagtggcctt gctggccaag gcccagcccc aggccatcat tcaggtcagc ctgttcaaca
     6181 cctcggcggc ctttatccgc gccatgcgca aaaccggctt cgccggtcag ttcctgaact
     6241 tctcggtggt cgggattgat ccgctgttca cggcgctggg caaggaaatc ggcggcgtgg
     6301 tcatctcgca agtcgtgcct tcgccgcgta gcgcgggaac ccccatcatc aaggagtacc
     6361 tggacgtgct gaacgtgacc gaccagacgc ccagttacga aagtgttgaa gggtatatcg
     6421 ccgcacgtac ctttgcggag ggcgtgcggc gcagcgccgc tggcggtgga aaagcagatc
     6481 gcgccgggct ccagaaagcc tttgaatcca tgaccgacta tgacgtgggc ggctttcgcg
     6541 tcaatctgcg tgccaaaaaa tacgaatcgg tccgtctggt ggatctggtg accatcaccc
     6601 cggacggcaa ggtggtccgc taggcgtaaa gcgcaccgca tccgcaagtg cataaaaggg
     6661 agaggggagg agggttcccc tatgatggcg ctgaaccaca gcgtacattg tcatggcccg
     6721 ccttccccgt ctgagccttc ccggctaccc gcaccacgtc atcctgcgcg gcaacaaccg
     6781 gcaggctatt ttttcgtgcc ccgccgatta ccagaccttg ctggatctga ttgaggaaca
     6841 cgccagaaaa tcaggcgtcg ccgtgcatgc ttatgtgttg atgagcaacc acgttcattt
     6901 gctggttacg ccggaggccg ccgattccct gccaagcatg atgcaggccg tgggcaggcg
     6961 gtatgtgcgc tatttcaacg acaggcaggg gcgatcgggc accctgtggg agggtcgcta
     7021 ccgttccacg ccgatccaga ccgaaaccta tctgctggcc tgcatggcct atatcgatct
     7081 caatccggtg cgggcagggc tggtcgccga ggcacgcgat tacccctggt ccagccacgg
     7141 ccactacgtc ggattgcgca gcgaccggct ggtaacacca caccccctgt tctggacttt
     7201 ggggaacaca ccttttgcgc gcgaagcggc ctacgccgac atggtgcgtg gcggtgtgtc
     7261 cgccagtcag caggcagcac tgacggaatc ggccctgagt ggctgggctc tggggaatgc
     7321 tgattttctg acggacctgc aaaaaataac agaacgccgg gtcgtcaaaa cgcgggtggg
     7381 acgcccggcg cgaaaaccag acggcccgtg agcggttttc caaattgggg ctgccacgct
     7441 aatttgcagt ttaatctgtc cccaattaat gcaatgagat tgcctgttcg ttttaattag
     7501 aatctgaccc caatttttct gcttggcatt gttgttgcac tgcactatgc tgtcagtcct
     7561 tccgatttat tacgaaactt ggcagacagg ctggtgtgca atgtgcactc cggcgctgtt
     7621 ctcccttacc gacccaggag taagccatga ccacggctgc tgaaatcacg cattttgaac
     7681 aaaacggtct ttatagcccg gccaacgagc atgacgcctg cggcgttggc tttgttgcgc
     7741 acatcaaggg tcacaaaagc catgccatcg tgcagcaggg tctgaagatt ctcgagaatc
     7801 tggatcatcg gggcgcggtg ggggcggaca aactgatggg tgacggcgcc ggcatcctga
     7861 tccagttgcc cgacgcgctt taccgtgaag agatggccag gcagggcttc aatctcccgc
     7921 ctccgggtga atatggcgtg ggcatggtgt ttctgcccaa agaacatgcc tcgcggctgg
     7981 cctgtgaaca ggcgcttgag cgtgccgtgc gcatggaggg ccaggtgctg ctgggctggc
     8041 gtgatgtacc ggtcaatcgg gagatgccga tgtcgccgac ggtgcgcgag aaagaaccca
     8101 tcctgcgcca gatttttatt ggccgcggca atgatgtgat cgtgcaggac gcactggagc
     8161 gcaagctcta cgtgatccgc aaaaccgcca gtgccgcgat ccagcgcctg aagctcaccc
     8221 acagcaagga atattacgtc cccagcatgt ccagccgtac cgtggtctac aagggcctgc
     8281 tgctggccga ccaggtgggc acttattacc tggacctgac cgacccgcgt tgcgtgtctg
     8341 cactgggcct ggtgcaccag cggttttcga ccaacacctt tcccgagtgg ccgctggccc
     8401 acccctaccg ttatgttgcc cacaacggcg agatcaatac cgtcaagggc aactacaact
     8461 ggatgaaggc gcgcgaaggc gtgatgtcct cgccggtgct gggcgccgat ctgcaaaagc
     8521 tttacccgat cagctttgcc gaccagtccg acaccgcgac tttcgacaac tgcctggaac
     8581 tgctgaccat ggccggctac ccgatcagcc aggccgtgat gatgatgatt cccgagccct
     8641 gggaacagca caccatgatg gatgagcgcc gcaaggcctt ttatgagtac cacgcggcca
     8701 tgctggagcc ttgggacggt ccggcgtcca tcgtgttcac cgacggccgc cagatcggcg
     8761 cgacgctgga ccgcaatggc ttgcgtccgt cccgctattg cgtgaccgac gacgacctgg
     8821 tcatcatggc atccgagtcg ggcgtgctcc cgattccgga aaacaagatc gtgcgcaaat
     8881 ggcgtctgca gcccggcaag atgttcctga tcgacctgga acagggccgc atgatcgacg
     8941 atgaggagat caaggccacg ctggcccaca gcaagcccta caagcaatgg attgaaaacc
     9001 tgcgcatccg gctcgacgat gtgcaggcga cgacggtgcc tgcgccgacg gacggcctga
     9061 agatgggttc cgacgaagcg agcgcgacct cggcgtcgag ctccggcttg accgagtcgc
     9121 actccttgct ggaccgccag caggccttcg gatacaccca ggaagacatc aagttcctga
     9181 tgtcgcccat ggccgtcaat ggcgaggaag gcaccggctc catgggcaat gacagcccgc
     9241 tggccgtgct gtccaacaag aacaagccgc tgtacaacta cttcaagcag ctgttcgcgc
     9301 aggtgaccaa cccgcccatc gacccgattc gcgaggcgat tgtgatgtcg ctggtgtcct
     9361 ttgtgggccc caagcccaac ctgctcgata tcaaccaggt caacccgcca atgcggctgg
     9421 aggtcggtca gccggtcctc aatgtcgccg acatgcgcaa gctgcgcgac atcgaaaccc
     9481 acacccaggg caaattcaag agctatacgc tggacatcac ctacccgctg gcctggggcc
     9541 acgaaggggt tgaagccaag ctcgcctcgc tgtgtgcgga agcggtcgac gcgatcaaga
     9601 gcggcaagaa catcctgatc gtcagcgacc gtggcatcag cccgacccag gtcgcaattc
     9661 cggccttgct ggcgctgtcg gccattcacc agcacctggt caaggagggc ttgcgcacga
     9721 cggcaggtct ggtggtggaa accggctcgg cccgtgaagt ccatcatttt gccgtgctcg
     9781 ccggttatgg cgcggaagcc gtgcacccgt atctggccat ggaaacgctg gccgaactcg
     9841 ccagggattt gcccggggac ctgagtgccg acaaggcgat ctacaactac accaaagcgg
     9901 ttggcaaggg cctgtccaag atcatgtcca agatgggcgt g
//
LOCUS       DAIDFG010000089         9937 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_89_length_9937_cov_19.247521, whole genome shotgun sequence.
ACCESSION   DAIDFG010000089 DAIDFG010000000
VERSION     DAIDFG010000089.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9937)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89_length_9937_cov_19.247521"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1686 a         3223 c         3285 g         1743 t
ORIGIN      
        1 ttcttcaagc ccagtccgcg catcatcatc tgcgtgccgt gcggctcgac ccaggtcgag
       61 cgccgcgcga tccgcgaaag cgccctgggc gccggcgcca gcgacgtcta cctgattgaa
      121 gaaccgatgg ccgcagccat cggggccggc ctgccggtct ccgaagccag cggctccatg
      181 gtcgtcgaca tcggcggcgg cacgaccgag gtcggcgtca tctcgctggg cggcatggtc
      241 tacaagggca gcgtgcgtgt gggcggcgac cgtttcgacg aagccatcat cagctatatc
      301 cgccgcaact acggcatgct gatcggcgag ccgacggccg aggccatcaa gaaaaacatc
      361 ggttcggcct tccccggctc cgaggtcaag gaaatggaag tcaagggacg caacctgtcc
      421 gaaggcgtgc cgcgcagctt cacgatctcc agcaacgaga tcctcgaagc cctgactgac
      481 ccgctcaaca acatcgtcag cgccgtgaaa accgcgctcg aacacacgcc gcccgagctc
      541 ggcgccgaca ttgccgaccg cggcatgatg ctgaccggcg gcggcgcgct gctgcgtgat
      601 ctggaccgcc tgctggcgga agaaaccggg ctgccggtgc tggtggctga agacccgctg
      661 acctgcgtgg tgcgcggctg cggcatcgcg ctggagcgca tggaccgcct cggctccatc
      721 ttcacctcgg aataaacctg gcgcatcctc ctcgatgcag ccggacaggc gcatgattga
      781 ccgcagacca tgcccttagg aaccctcgac agaagcccgc cgcccttttt caagcaggga
      841 ccgtcggccc tgtccaagct gatgtttttc agcgcattgg ccctgttcct gatggtggcc
      901 gacctgcgtt tcaagatcac ccagcccatc cgggcggccg tggcgacggt gctttacccc
      961 gtgcagtggc tggccctgca gccggtccgc gcggcccggt atggcggcgg ttatttcacc
     1021 gcgctgagcc aggcccgagc cggcgaagaa gcggcccacc agaaactggc gacgctgtcg
     1081 ctgcgcgcca gccaggttga gcagctggca ctcgaaaatt cgcgtctgcg aaagctgctt
     1141 gatctgcgcg aacaggcggc cctcacaggg caggccgcac aggtgcttta cgacgccccc
     1201 gacccctaca cccgcaaggt catcatcgac aaaggcctgc ttcagggtgt ggcgcttggt
     1261 tcgccggtgg tggacgagtc cggtgtgctg ggccaggtca cccgtgtcca tcccctgatc
     1321 agcgaggtca cgctggtcat tgaccgcgac cttgccatcc ccgtgctcaa cacccggacc
     1381 ggtgcgcgca gtgtggcctt cggcgagccg accacccatg gcggcgggct ggagctgcgt
     1441 ttcatgggca gcaattcgga tgtgcagcag ggcgacctgc tgacgaccag cggtgttgac
     1501 ggcgtttatc cgccggggct gccggtggcc aaaatcgacc ggatagagcg gcgtgccgaa
     1561 tcggcgtttg cccggattta ctgcacaccg caggcccagg tcaatgccgc tttccatgtc
     1621 atggtggtca agcccttgtc cggcgaaaag ctgccagctg ttgccgcaga gcccgcgccc
     1681 gtacccgtca aaaaaggagc cggcaaatga tcatgccctc tggccagcag ctgctgctgc
     1741 ccgccaaccc ggtgttcatc tggaccagcc tgatcgtggc cctgttgctg gacatgcttc
     1801 cgctcgggcg ggtgccctgg atgccggatt tcctggcact ggtgctggtg ttctggaaca
     1861 ttcaccagcc gcagcgggtg gggattggcc tggccttcat gttcggcctg ggcatggatg
     1921 tgcaccagtc ggccctgctc ggccagcacg cgctggccta caccgccctg agctttttcg
     1981 ccaccatgat ccaccgccgc ctcctgtggt ttaccgtgcc ctcccaggca ctgcagatcc
     2041 tgccgctgtt tgcggtggcg catggtgtgg aaatcaccat ccgcatgatg agcggcggtg
     2101 tttttcccgg ctggacgctg ctgctggcgc cgctggccga gtccctgctg tggccggtga
     2161 tcagcgtgat gctgctggcg ccgcagcgtc gggcgcccaa tcccgaccag aaccggccgc
     2221 tgtaggcatg cccccacggt tgctcactgc gtgtagcgcc ctgccccccg ggggggccgc
     2281 tgcgcctgcg gactggcaaa gccagatccg cggcccctgc tggtctgaag agtccccgca
     2341 gtcgctgcgc cagcgggctg gccaagccga ctccggagcg gagcttggtc tggagatggg
     2401 acgcattttg caagtggggg cactttttcc gccggccctg agtcctactt gccatgactg
     2461 aactcagaaa cgtcgaggct gatctttcac gtttccgtgc gcgggtgctg gtggccgggc
     2521 tggtcatcct gttcgccttc gtgctgctgg ccgcccgcct ggtctttttg caggtggtgc
     2581 ggcacgacga cctcaacgaa caggccgaga gcaaccgtac cgcggtcgtt cccatcgtgc
     2641 ccaaccgggg cctgatcctc gaccgcaacg gcattgtgct ggccaccaac tattcggcgt
     2701 atacgctgga gatcacgccg tccagggtgg ccgacctgga ggaaaccatc gaggcgctgg
     2761 ggcggctggt ggatattcat ccgcgcgaca agcgccgttt caagaagctg cgcgaggagt
     2821 cccgcaattt cgagtcactg ccgatccgca cgcggttgac ggacgaagaa gtggcccgtt
     2881 ttgcagccca gcgtttccgc ttccccggtg tcgacatcaa ggcgcggctg tttcgcagct
     2941 acccgaatgg tgagctggcc agccacgtgg tcggctacat cgggcgcatc aaccggtcgg
     3001 aaaaggaacg gatcgaggaa aacgacgagg aaggcaacta ccgcggtacc gactacatcg
     3061 gcaaactcgg cgttgaacag agctttgaag cccagctgca cggcaacacg ggcgtcgagc
     3121 aggtggagac gtcggccggt ggccgcgcgg tgcgcaagct cgccagtacg gcggcggtgc
     3181 cgggcgatac ggtgatgctg tccatcgaca tcaagctgca gaagctggtg gaagacctgt
     3241 ttggcgacag gcgcggcgcg ctggtggcgc tggacccgtc caccggggaa gtgctggcct
     3301 tcgtcagcaa gccgacattc gatcccaacc tgtttgtcga tggcattgac agcgaaagct
     3361 ggcaggccct caacgagtca atcgacaagc cgctgctgaa ccgggcactg cgcgggacct
     3421 acccgcccgg ctccacctac aagcccttca tggcgctggc ggcgctggag tccggcaagc
     3481 gccagcccgg cagcatcacc aacgacaccg ggtcttggat gtttggcggc caccagttcc
     3541 gcagccatgg ggccgggctc ggcccggtcg acatgcacca gagcatcgtc aagtcgagca
     3601 atgtctacta ctactcgctg gccaatgacc tcggtgtgga tgccatgcat gacttcatga
     3661 agccgctcgg cttcgggcag atcacgggga tcgacatcca cggtgaagtg cgcggcgtgc
     3721 tgcccagcca ggaatggaag cgcaagacct acaagcgggc cgatcagcag aaatggtacc
     3781 caggcgaaac catttcgctg ggcatcggcc agggttacaa cgcgttcacc atgctgcagc
     3841 ttgcgcaggc caccgccacg gtggcgaaca acggcgtccg gcacaaaccg cagctggtga
     3901 ttgccacgca ggacccgatg acgcgggcca tcgaacccat tgctccggag ccgccgcaaa
     3961 acctgggcta caagccggaa aacgtggcgg tgatccgcaa ggcgctggtc ggtgtcacgc
     4021 aggagggcac gtcgacccgg gtgtttgcgg gggccggcta tctcagcggc ggcaagaccg
     4081 gtacggccca agcggtgggt gtcggccaga aggacaagta cgacgcccgc aaacttgaag
     4141 agcaccagcg ggaccatgcc ctgtacatgg cttttgcgcc ggcagaccat ccgaaaattg
     4201 cgctggccgt ggtggtggaa aacgccggct tcggtgcagc ccatgcggcg cccattgccc
     4261 gacgcgtgtt tgactacgtg ctgctcggcc agtaccccag cgaggtggac atggcggccg
     4321 tgcaaaaggg gctggcggct gcgcccatcg gcaagccgcg cctggcggcc gaaatggcgg
     4381 gcttgctggg gagcggcacg accacagcca ccaatccggc caaggccgcg gcgattgccg
     4441 aagccctcag caagctgccg ccgcctgtgc ctgtgcctgt gcctttgccc gacgccgatt
     4501 agcgccgttc ttgcgctatt tcaggaaagc gtcgaatccg gttttgagaa tcagtgcgct
     4561 gaccaccacg ataaacacca cacgtacaaa acctgcgcca tgcttgagcg ccaggcgtgt
     4621 gcccagcagg ctgcccacca cgttggccag tgccatggtc aacgcgaaat gccaccagac
     4681 gtggcccttg tacgcaaaca gcagcagggc ggccaggttg gtggccgtgt tgagcagctt
     4741 ggcggacgcc gaggcgctca ggaagtcgta acccagccag cgcacgaaca ggaagacaaa
     4801 aaagctgccg gtgcccgggc cgaaaaaccc gtcgtaaaac cccagcagga taccgatggt
     4861 gcaggccgcc caggtttcgg cgcgaccgga aaaccgaggt gcgtgggtgc gccccaggtc
     4921 cttgcggacc agggtgtaga gcagcacggc cagcagcaca aacggcagga tcttgcgcag
     4981 aaaccccggg gaaatcacgg tgaccagcca ggcgccggcg aacgagccgg caaaaccggc
     5041 cagcgcggcc ggcagcaggg cggcccagcg catgtccacg cgccgggcgt attgcacggt
     5101 ggccacagcg gtgccccaga ccgatgcgcc cttgttcacg ccaaacagcg tcgccggatg
     5161 ggtggtggga aacacggcga acagggcggg caccagaatc agcccgccgc cacccacgat
     5221 ggaatccaca aaacccgcca gcagcgaggc caatgacacg atcagtaatt ccatgggtga
     5281 attgtctcat tggccccgtc ggcggcaggc tgccggtccc gacctcgcac ctcggatgat
     5341 ccggatcggg cgggcaaaaa aaagcgccgg gttcaccggc gctttgtaaa aaacatctct
     5401 ctggatgcca attctgttgt ctgttgtgct tgtgcgagtt tgtctcctcg gccctctgcg
     5461 agcggcttgg ctcgtatgca gatcactgaa tgtagtgtgc gcacccgcat agtgcatcgg
     5521 gactttccct tccccgtgtg gggggggctg ttccctgctg gctgggcgcc agggtgcggt
     5581 gaaacagggc gtcggcagct tggcacgact tatgcctgtt acgagatgtt cgatccattc
     5641 agcacacatc ggaaatcacc atgcaccaca cgcggttcgt ttcattgggt tgggggctcg
     5701 ggctgctgtt ggccgccacg ctggccggag cacagacgcc ggctcctgca gaggccgcgt
     5761 ctgccgtgcc agcagccagc gcgcctgtcg aaccggtggc gcaggtggtg gttgcgccgc
     5821 ctgccacaac agcggctcca gccccagctc ctgtccctgc ggtcccggtc cccgcccctc
     5881 tgacccgccc gggtctggtg gccgccgata ccctcaatgg cgccgacacc gcctggatga
     5941 tgacatcgac ggcgctggtt ctcctcatga ccttgccggg catcgccctg ttctacggcg
     6001 gcatggtgcg caagaaaaac gtgctcaaca ccatggcctg cgtggtggcc atttgcgcaa
     6061 tcgtcagcct gctgtggttt gccgtcggtt actccctggc cttcacgccg ggcagcggcg
     6121 cgctgggcca ctacattggc ggtggcgagc ggctctggtt tgccggtctg gactatgtca
     6181 aggacgcgca gaaagtcgcg gtcagccata ttgcaccgaa tatccccgag tcggtgtacg
     6241 cgatgttcca gctgaccttt gccatcatta ccgcggcgct ggtggtgggg gcgtttgtcg
     6301 agcgcatgcg gttttccgcg atgctgtgtt tcatcagcct gtggaccctg gtcgtatatg
     6361 caccgattgc gcactgggtc tgggaaccca atggctggct ggcccaactt ggggcgctcg
     6421 attttgccgg tggctccgtc gtccatatca atgccggcgt agcggggctg gtctgtgcct
     6481 acgtgctcgg accgcgccgc ggctacggcc gtgaggcctt cacgcccttc aacctcgggc
     6541 tcaccatggc cggtgccggc atgctctggg ttggctggtt cggattcaat gccggttccg
     6601 ccgtggcctc cgacggccgg gccggcctgg cgatggcggt aacgcacatt gccgccgctg
     6661 ccggcgccct gtcatggatg gcggccgagt ggatggtccg caagcggccc accttgcttg
     6721 gactgtgttc ggggctggtg gccgggctgg tggccatcac cccggcggct ggctttgtct
     6781 cgcctcgcgc ggccctggcc atcgggctga ttgccggcgt cgcctgctac tggggcgcga
     6841 ccggcctcaa acgcctgctc aatgccgacg attccctcga cgtgttcggc gtgcatggcg
     6901 tgggcggcat cgtcggctcg ctgctgaccg gcgtgtttgc cagcaaaacc atctcgggcg
     6961 tggaaggcag cgtgttgacc caggccatcg gtgcaggcgc cgtgctgtgt tacagcctgc
     7021 tcatgacggc cgtggtgctc tggatcacgt cgctggtttt cagcctgcgg gtcagtgaca
     7081 gctcggagtc cgatggcctg gacattgccc agcatgccga gcggatgggc acctgatgga
     7141 ggatcccgac atgccttcca gcgaaaaaat tgcgattgct gcccacttgc atgtattgct
     7201 gcgcagaaag accgggcggg tgaccgacac cgagtggatg gcgaccaatg cggaatacgc
     7261 ggccgaaatc gtccgtttct cgcgcgagaa ggccgtcgaa gatggccata tcgatctcgg
     7321 cgtctgggcc gaccggctcg aggaaatcat ggcgtctccg gaggccaggc accgcgtgcc
     7381 gctggtcagg atgatggccg atgcggtcaa ggagcgcagt gccgcgccac cagcgggcgc
     7441 cgcgccggtg ccggatcagc agggggcccg ctcggtcaac accgaggaag aggtctaccc
     7501 cgagtcgggc ttcatcgagt cgacgttcgg caactttttt ggtgacagcg aaaaacaccg
     7561 cgagcgcaag ccgcgcgatc cggatgcccc ccgttatgtg aagggactgc gctgagcggc
     7621 tctcgcctgc ggcacctgtt tcacatgcct agcgtcgaag gggaacggct ggagctgcgc
     7681 gtccagcgtt tggcgccagc cagtgtgaaa taaaaacagg tgccctgccc gggtgccgat
     7741 gtggcccaga tccgtccgcc atggcgcgtg acaatccggc gtgcggtcgc caggccaatg
     7801 ccggcaccgg caaattcgcc tggggcgtgc agtcgctgga aggcggtaaa cagcttgtcg
     7861 gcatacgtca tgtcgaaccc gacgccgttg tccctcacga aaaaaatcat ctcgccatcg
     7921 gtacctggct cgccgcccac cgtgatctcg gcgtccgccc gcgtagacgt gaacttccag
     7981 gcattgccca gcaggatgtt cagcaaatgc agcgccagcc cggcgtcggc ctgcacggcc
     8041 ggcaaggcct ggacccgggt ggccacaagg cgttccgggt gctgttcctg gagtgatttc
     8101 agaaccgcgc cggctatggc gcccaggtcc acctgctcgc ggcgggactg cgtgcgcgac
     8161 agctgcgcca gcgccagcag gctgtcggtt cgttcgttca tctggctgac cccggcacgg
     8221 atgcgctgga ggtagtgcct gccgcgctcg ctgatgccgg ccgccatttc cttttccagc
     8281 agccgggtca tgccgttgat ggcaatgtag ggctggcgca ggtcatgggc cagcgcctgc
     8341 gagaaagcct gcagctccag ggtggctgcc gtcagttcgg ccgtgcgttt ttccacgcgg
     8401 gtttccagct ccgcattgag ctgcaggatc tgctgctcgg caagcttgcg ttcggtgatg
     8461 tcgcgcagga tcacgctgac ctgcacgtcg ccgtcgctgt tcacatgctg cgacgacgag
     8521 gcctcggccg gaaaacgcgt cccatccccg cgcacacaga cgagttcgcc gcggaacctg
     8581 gcggagcggc tgatctccat taccagtccg gccatgcgtg gatccgtgct gtccaccaga
     8641 ccggcaatgc ctgccgccct gatttgctgt tcgctcatcc cgaacatggt gcaggcggcc
     8701 tggttggccg cgaccaccgt gccatcgggc aggctgtgca ggaagccgtc caggctggtt
     8761 tcgaccacca gccggaaccg ctcctcgctg atgcgcaggg tgtcctcggc tgcgagcagg
     8821 ctgcggtggc ggcgtgcccg gctctgcgcc gcagagacca gcgtttccgg ggagacgggc
     8881 ttggtgatga agtcatcacc accctgccgg agcgcttggg cctgctggtc caggctggtc
     8941 tcggcagaca ggaagatgat gggcacccgg acgaactcgg gctgctgccg gatcagggtc
     9001 gccagctcga tcccgttgca ttgcggcatg tggatgtcgc aggtgatcag gtcgggctgg
     9061 aaatcggcca gcggcgccat gacctgacgg ggctcggtga cgacgcacgt ttgcatgcct
     9121 gcggactgca acacgccggc ataaaagctc gccagtgttt cgtcgtcctc gatgatcagt
     9181 acccggtagg gctgcgtcgg ctccgcagag gtgagcaggc ccagcagggc ggtgatggcc
     9241 cgcacatcca cggggtgcgg gaaataaccg gctgccccgg cccgcacggc ggccaggcgg
     9301 gtctggaagt ccgcgccggc gcccatgaga aggaggggcg tccggccttc tgtggccggg
     9361 ccgggcgcgg ccagtacggg ccacgccacc agggggtctg cggaaaacgt gatgtcggca
     9421 agaatggccg ccggcgcttc ctgccgtgcc gccaggtctg cggcagcagg ctgggtgaac
     9481 acgcggatct ggtagccgcc atggctcagc tgatcggcaa gctggccagc cgctaccccg
     9541 tcgggatcga tgatgtgaag cagccgtgct cgcccggtgg gctggggcac gacggcatga
     9601 ctgctggccg tgagaaccgc agcgggagtc tcaacggcag gctggtccgc ggccgccgca
     9661 gctttcagcg cctgcaggcc ggcttccagc gagagacgat actcaacgct gggaaaaccc
     9721 gggacaccgg cgtttttcga gagaagtttc tcgatcgccg ccgccgcttc gctgacggcg
     9781 ggaaaaccaa aggtgccgcc agagcccgcc agcttgtggg cctgcgccag cacggacagc
     9841 gccgcctcct ggcttgcggg ttcgtcggtg ccgccgtcca gttgtcgcag cgctgcttcc
     9901 agcgcactga tcttttccgg caacccggcc gcataca
//
LOCUS       DAIDFG010000090         9912 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_90_length_9912_cov_17.714112, whole genome shotgun sequence.
ACCESSION   DAIDFG010000090 DAIDFG010000000
VERSION     DAIDFG010000090.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 9912)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9912)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9912
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90_length_9912_cov_17.714112"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1446 a         3400 c         3305 g         1761 t
ORIGIN      
        1 gcggcagcaa cacgcggtcg cgctcgtcgg tcaggccgtc gccattgagg tcgtcgggtt
       61 tgccgtcgcc gttggcgtcc acctgccgcg cgggcaacag gtaggccttc caggggccga
      121 tgccgtcggc gtaggttttg atctccttca ggccggcggg cgtcagcagg tcggcaaaag
      181 tgcggctgtt gttggcaagg gcgaagtcgt aaggcttgtc gaacggcggc ttgagcgtga
      241 cctggccctg ggcgttcacc ccgtcgccgt cgatcagctg caccaaacgc acggacgtct
      301 tgctgcgcag gtatttcagg ttggccactt caaacgactg cacgatgacc ggcgaacttt
      361 tcgtggtgta gccgtactcg gccagcagcg cgagcaagcg gtcttccagc gccagcccgg
      421 cgttcacgtg gtaggtcggg tgtttggtct cggggtagac accgaccgtg cggccggcgc
      481 gtgcgccttc ggtgcgtgcc agttcgagca cctcgcgcag cgtcgggatc gcgtatttac
      541 cgttgtggga ttggtcgcgc tcggccatgg gctgccgggt acgcagggtc ttgatctcgg
      601 ccagcgtcag gtcggtggtg aaccagccgg tttccgccac cccatcgacc tgccgggtgg
      661 tcttgcggct cgcaaactcg ggccgggtcg acacgtcggt ggtggccgtg atgttgggct
      721 cgtgccgcac cacgagttca ccgtctttgg tggccaccac atcgggctcg atgaaatcag
      781 cgcccagctc gatggcgcgt ttgtacgatt ccagcgtgtg ctccggcaga taaccgctgg
      841 cgccgcgatg cccgatgacc agcggcgcct tgccgtccag cgtgggataa cgacccgttg
      901 cagcggtggt cgagccgccg gcacaggcgg ccaggcccag gaggccgaca gcggccagac
      961 cggccgcgag gatgcgcaat gaaaccatgg gtcgtctccg tgaatgaatg tcccactatg
     1021 gtgcatgcaa gtgacggttt gatgaagcgc tgttgcaagg gcttatcccg caccttgccg
     1081 ccggctttgc gggagcttca cacgatagac acggcagaaa gcctgcttgc cgtagaaaat
     1141 tcctgataac gcgaactttt tacctctctg gccactcagt catgcttatg aaacgctgga
     1201 ccctgctcgc cgccaccgta ctggctctcg gcattgccgt tctcgcgggc tgcgccacgc
     1261 tcgacgagcg gcagcgcgcc tggatcttcc agcccagcga ccgcagctgg agcggcggcc
     1321 tggccgctgc cgaaggcatg gaagacgtct ggatagggtt caaggccaaa gccagcggtg
     1381 acgaacaggc caccctgcac gggctgtggc tgcccgccga acagccaaag gcccccgtgc
     1441 tgctttatct gcacggtgcc cgcttcaatg tgaccggctc ggccccgcgc atgcgccgca
     1501 tgcaggagct gggcttttcg gtgttgggcg tggactaccg tggcttcggc aaaagcaccc
     1561 aggccctgcc ctcggaagag tccgcctacg aggatgcccg cgccgcctgg gactggctcg
     1621 ccaagaaata tcccgaccgc ccccgttatg tctttggcca ctcgctgggc ggcccgattg
     1681 ccatcaacct cgccaccgag gtcaacgacg aaagcggcac gattgtcgaa ggcaccttca
     1741 cctccattgc cgacgtggtc agcacctcca aatggggctg gatgccggtg tcgctgctga
     1801 tcacccagcg tttcgaggcc gcccgcaagg tcggcaagat cggctcgccg ctgctggtgg
     1861 tgcacggcgg tgaagaccgg ctgatcgccc ccgacctcgg gcgcaagctg tacgaggccg
     1921 ccgcgcagcc caagctgttc atgctggtcg agggcggatc gcaccacaac accaactccg
     1981 tcggccaggc gcagtaccgc caggcgctga cgcagctgtt cagcctgaaa tagcccagcg
     2041 ctggcaaggc acccgctccg cgcagccacc cggcgcgcag cgatctgata cgctcagatc
     2101 aatgtccgcc acaccccctg ctcctgactc cgccgccccc gcggtgaccg ccgaaccgcc
     2161 agccatgtcg cccgcgctga ttgtgctggt gctgaccctg ctgctcggca tccagcccat
     2221 caccaccgac ctctatttgc cggcgctgcc cgccctgacg gaaggttttg gggcgccgat
     2281 ggcgcaggcc cagctcacgc tcaccgggct gctgctggcg tttggcgttt cccagctttt
     2341 ctggggcccg ctgtccgacc gttttggccg ccggcccatc ctgctgtggg gcctgggcgc
     2401 ctacgtgctc gcggccatag gcgccgcgct cgcgccgtcc atggcgctgc tgatcacctg
     2461 gcgcatcgtg cagggcgcgg ccatgggggc ggccgtcatg tgcgcgcgcg ccatcgtgcg
     2521 cgacctctac acgccggtgg ccgccgcacg cgccatgtcc cgcggcctga gcggcctggg
     2581 cgtgattgcc tgcctgagcg cgccgcttgg cggcctgctc tcggacgttt tcaactggcg
     2641 cgcggcgctg ctggcactgg ccgtgttcgg cgccgtagcg ctggccatga ttgcgctgcg
     2701 cttcaaggaa accctgacgc tgaaaaaccc gcgcgcgctg gagccggcca cgctggtgcg
     2761 cacctggtgg acgattgtgc gcaacccgac ctttgtggcc ttttcggcgc tgtccacggc
     2821 ttcctacggc gggttgttca cctttctggc cgcctcgtcc tttgtgttta tcggcgtgtt
     2881 ggggctgagc aagacgcagt acggcctggt gatgttttcg atgtcctttg tctatgtgct
     2941 gggcaccttc tggtgccgcc ggttgctgcc gcgttttggg gtgcggcgct cggtggcgat
     3001 tgccggcgtc ctgacgctca gcggcggcct gctgatcagt accctggcct gggccggcgt
     3061 ccataacgtc tgggccctgc tgttgcccta ttacctgttt gtgtttgcgc acggtgtgca
     3121 ccagccctgc agccagagcg gcgccgtcag cccctttccg ggtgcggccg gcgcagcttc
     3181 ggcactgaac ggctttctga tgatgctggc ggcctttgcc atgggcggct ggctgggcag
     3241 ccacctggac ggcacggtgt tcccgctggc ttatggcgtg ttgttctgga gcgtgtgcat
     3301 ttccgcggtc gcctggacac tggttcaaaa acacggcgac cctgcaacgc cgggcctgcc
     3361 ccagcggcca tgaccaaacc ccgctggatc ggccttgccg gacccaccgc gtcgggcaaa
     3421 accgccgcgg ccttcgccat tgccgggcgc cacccggtgg aaatcatcag cgtcgattca
     3481 gcgctggtct accgcggcat ggacatcggc acggccaagc caacacccgt cgaactcgct
     3541 accgtgccgc accacctgat caacatccgc gacccgctgc aggcctacag cgcggccgag
     3601 tttgttgccg acgccacgcg cctgatcgac gacatcgcag cgcgcggcaa gctgccgctg
     3661 ctggtgggcg gcaccatgct gtatttcaag gccctgctcg acgggctcga cgacatgccc
     3721 aaagccgacc cggcgattcg cgccgcgctg ctggccgaag cgggcgagaa aggctggccc
     3781 gcgctgcacg cggagctggc cgcggtggat gctgtgaccg ccgcgcggct ggcgcccaat
     3841 gactcgcagc gtatttcccg cgcgctggag gtgtttcgcc tgaccgggct gccgctgtcg
     3901 tcgttccaga aaaaaaatgc tatcgaaaaa gtagctggtg gcgcacatcc cataagggct
     3961 ggagccctga tttcccttga accgcaagac cgcgcctggt tgcacgaacg catcgcccag
     4021 cgctttgaca ccatgctggc cgccggtttt ctcgacgagg tgcgggccct gcgtgcgcgc
     4081 ggcgacctgc accccgacct gccgtccatg cgctgcgtcg gttaccgcca ggcctgggaa
     4141 gccctggacg gcgccttccc catgagcgaa ctgcgcgagc ggggcattgc cgccacgcgc
     4201 cagctcgcca agcgccagat cacctggctg cgctccatgc ccgagcgcca ggtggtcgcc
     4261 tgtgacgcag cagacgcggt gcagcaggtg cttgatacgg tggccgcgtt cagtgcaact
     4321 tgatgccagc aactttttca gacctgcagc gcctgcgctg tcatccggac tcgatccggg
     4381 atccatgccg catgaaccac gtcagcagct tgcgcagcat ggattgcggg tcaggcccgc
     4441 aatgacaaat ctacattgca tgggagcgtg tgcttgagcc tcgtcgtcca gaacctcagc
     4501 aaacactacg gcgacgtcgt ggtgttcagc aacgtctctc tgcaggtcgc acccggcgag
     4561 tttgtcgcca tcgtcggcga gtcgggtgtc ggcaaatcga cgctgctcaa ctgcatggcc
     4621 gggctggaca gctgggacgc gggttcggtg tcgctcgatg gcgtggacct gggcgcgctc
     4681 agcgacgacc agcgcgccct gctgcgccgc caaaaagtcg gcttcgtctt ccaggccttt
     4741 catgtgttgc cgcacctgga cgtcgcgcaa aacgtcgccc tgcccctgtt gctgctcggc
     4801 cagcacgacg acgatcgggt gcaggccatg ctggccgccg tcgggctgga aggcctgggc
     4861 acgcgcctgc cgcagcagct ttcgggcgga cagttgcagc gcgtggcgat cgcgcgcgcc
     4921 ctgatccacc ggcccagcct gctgctggcc gacgaaccga ccggcaacct tgaccccacc
     4981 accgccgcca gggtcatgga tgcgctggtg gcgcaaaccc gtgaacacgg cgccgcgctg
     5041 gtgctggtca cccactcgga aaccgctgcc gcgcgcgccg accgtgtgtt gcatctgagc
     5101 agcgaaggca tcacgggctg acgccaggca gaccttcccg aagacaatga cttgtcaaag
     5161 tctccatcag ccccaactga atcctgatga acgcctcatc ctttgaaacc ctggagttgc
     5221 tccccgcaac ggcgccgcgc cagttgtttg tgctgctgca cgacgctggc ggttcggcca
     5281 tggacatgat ggagctggcc acactgctcg gcgagtcctt cggtgaagcg gccgtcgtga
     5341 tgccggaagg cgtcgaatcc atccacctgg gcaagaccga gcggcagtgg ttcccgacct
     5401 ttgaactgac cgacgccaac cacccggaac gcgtggcccg ggcggtgccg accctggcgg
     5461 ccttcattcg ccagcagcag gagcgttttg gcatcctgca gtccgacacc gcgctggccg
     5521 ggttttcgca gggcgccacc ctggcgctgg cactcagcga tcagcacgac ggcctggtcg
     5581 gtcgcgtgct ggctttttca gggccttatg cggcctggcc cgacaaggcc cctgcgctca
     5641 cgaccattca tttgctgcat ggccagctcg acaccgtggt accggcgcgg ctggcccagc
     5701 aggcctatgc cgaactgatg gcgctgggcg ccgacgccac ctgcgacatc gcttcgagca
     5761 tcggccacga gctgcacccc gcactgatgg cgcaggccgt gcaccggttg cagacctgcg
     5821 tgccgctgcg cttctggcgt tcggcctgac cctgcttaac gcccaaccag cagatcggcg
     5881 taacgcgcca gatccacatt gcccccgctc acgatcacgc cgacgcgagc acccttgatc
     5941 gccgccgcgt ccgtgcaggc gccggcaaac gccaggcagc cggtcggctc caccaccatc
     6001 ttcatgcgct ctgcaaaaaa gcgcattgct tccaccaact gcgcatcact gacggtcagg
     6061 atgtcgtcca catcgcggcg gatgatgccg aaggtgtact cacccaggtg ctgggtctgc
     6121 gcgccgtcgg caatggtctg gggcacggga atgtgcacga tgcggccggc acgcagcgac
     6181 tgctgcccgt cgttgccggc ttcgggctcc acgccataga ccttgcactg cggcgccagc
     6241 gcgcgggcgg caagcgccga gccgctggtc aacccgccgc cgccggtgca cacgtacagg
     6301 cagtccagcg gtccggtttc ctcgaacagt tccttcgccg ccgtgccctg gccggcaatc
     6361 acatcggggt ggtcgtaggg cggaatcaag gtcatgccgc gctccgccgc cagccgcagc
     6421 gtcaatgcct cgcggtcttc ggtgaagcgg tcgtacagca ccatctcggc gccgtagccc
     6481 cgtgtggccg ccagcttggc tgccggcgcg tcctggggca tcacaatcag cgccggcatg
     6541 cccagcagcc gtgccgacag cgcgaccgcc tgcgcatggt tgcccgacga gaaggcaatg
     6601 gcaccggcct tgcgctgcgc ggcatcaaac cgcgacagcg cattgaaggc cccgcggaac
     6661 ttgaaagcac ccatgcgctg gaagttctcg cacttgaaga aaaactgcgc accccagcgc
     6721 gcatcggcag tggccgaccg cagcaccggt gtgcggtgcg catggcctgc tatccgcgcg
     6781 gcggcggcaa caacatcgtc gtaggtaggg agctgcatgg cgagggggct ttcattcaag
     6841 ggttggtgaa caacgatagc ctagaacctg accgtcatca ccgccagcac cccgaaaggc
     6901 acaatcaggc gcatgctcgc ccttctttcc accttctcct ggcaggagct gctgcaccac
     6961 aagtggcgca acgcggcggc cgtggtggcc gtcatgctgg gtgtggcgct ggcgttttcg
     7021 gtgcagctga tcaatgcctc ggcactcagc gagttctcgc aggccgtgcg ttcggtcaac
     7081 ggccagcccg acctggagct gcgcgcggtg cgcggcgggt ttgacgaagc ggtttacgcg
     7141 cgcgtggcca accacccgca ggtggcgctg gccagcccgg tgctggagtt cagcaccttc
     7201 gccgtcgccc ccggcggcca gcgggtcgcc ttgcgcgtgg tgggtgtcga tgcgctggtg
     7261 gtcgcctcgc tggcgccggc cctgatgccc gtgccctcgg ccggggccga acgcctggcc
     7321 ctgctcgccc ccggtgcggt tttcctcaac gcggccgcgc ggcaaagcct gcccggcgat
     7381 cggctgcagt tgcaaagcgg gctgcagttg ctggacgcgc gggtggccgg cagagtcagc
     7441 gccgagggcg ccgcgctggc ggtgatggac ataggcgcgg cgcaggaact gttcggccgg
     7501 ctcggccagc tctcgcgcat cgacatccgc ctgcaggccg gcgccgaccg cgccgccttc
     7561 atcagctcgc tgcaactgcc ggccggcatc accgccaccg agccgggcga cgacgcgcag
     7621 cgcgtgagca acctgtcgcg ggcctaccgc gtcaacctca cggtgctggc gctggtggcg
     7681 ctgttcaccg gcgccttcct ggtgttttca gtgctgtcgc tgagcgtggc caaacgcgcg
     7741 cagcagtttg ccctgctcgg cgtgctgggc ctgaccggcc gcgaacgcct gcgcctggtg
     7801 ctctgggagt cggcgctgct gggcacggtc ggcagcgtcg ccggcattgc gctgggcacg
     7861 gcactggccg cgctggcgtt gagggtgctg ggtggcgacc tgggcggcgg ctacttttcc
     7921 ggcgtggccc cggccctgca gtggaatgcg ggcgcggccg cggtgtatgg cctgctcggc
     7981 gtggccgcgg cgctggtcgg cggctggtgg ccagcgcgcg ccgcgcaaaa gttgccgcca
     8041 gcgcaaaccc tcaagggcct gggcgcgccg ccggccggcg gtacgcctta ccggctcagc
     8101 ctgctgctga tggtcgctgc cggcctgctg gccttgacgc cgccggtctt tggcatcgca
     8161 ctcggcgcct acctgtcggt cgggctgctg ctggtcggcg gcatcagtgc gctgccctgg
     8221 ctgatcgccc tgctgtacga ccgcatcgcc ccgcacgtgt cgcaccgcct gttgccgctg
     8281 ctggcggtgg aacgggcgcg gcgtgtgcgc ggcagtgcgg cggtggccgt cagcggcgtg
     8341 gtcgcctcgc tgagcctggc cgtggcgctg acggtgatgg tggcgagttt tcgcgactcg
     8401 gtcacccact ggctggacgt ggtgctgccg gccgacctgt acgtgcgcac cgccggcacc
     8461 accgccgcca gcgacacggt gtttttctcg cccgcgtttg tgcaggcgct cgcgcagctg
     8521 cccggcgtgg cgcgtctgaa cacgctgcgc gtgacttccc tgctgctgga ccccaaccag
     8581 cccgcggtgg cgctgattgc gcgcgacctg caggaccccg ccggcagcct gccgctggtg
     8641 ggcagcgcgc tgccggtacc ggccggtcac gtggcgattt atgtgagtga acccatggtc
     8701 gatctgtacg gtgcccgccc gggcaccgtg ttttcccaac tttcagggtc ttttgaggct
     8761 ctggcccttg atggacgggc gcgaggagct atgaattcag gagcatccca ggctaacccc
     8821 aagttctacg tggccggtgt ctggcgggac tacgcgcgcc agttcggcgc catcgccatg
     8881 gacaggcgcg actttgaagc cctcaccggc gaccggcgcg tgaacgacat cgccttgtgg
     8941 cgcgcccccg gcgcgggcga cgcgcaactg cagcaggccg tgcgcgcgct ggcgcaggcg
     9001 cagacgggct cgggcgagct gatcgagttt gcgtcgagtg gccagatccg cgccacctcg
     9061 ctgcgcattt tcgaccgcag cttcgccgtg acctactggc tgcaggcggt ggcgattgcg
     9121 atcggcctgt tcggcgtggc ggccagcttc agcgcgcagg tgctggcgcg gcgcaaggag
     9181 tttggcctgc tggcccacct ggggctgacg cggcggcaaa tcctcacgct ggtcgccggc
     9241 gaaggcgccg cctggacgct ggccggcgcg ctggccggcc tgggcctggg gctggccgtg
     9301 tcgctggtgc tggtgcacgt ggtcaacccg cagagttttc actggaccat ggacctggcc
     9361 ctgccctggg cgcggctgct ggcgctttgc gcggcggtca ttctggcggg caccgtcacc
     9421 gcctggctgg ccgggcgcgc agcggcgggc aaggacgcgg tgcttgccgt caaggaagac
     9481 tggtaagccg attcgccagc tttgcggccg gcggtttgct gccggctatg cagcgacagt
     9541 ggacgatgcg cgctttcttt tgcttaccaa gatgacgctt tgaatgcggc attgtcatca
     9601 caacgccgca tgatctgcct acgatgggca catcacttca acaaggcatt caccatggcg
     9661 cccgctcaat accgcattga aagcaggaac acgctgcggg gcagcctgct cccgaacaac
     9721 gccctcaggc agctctacac agaccgctct ctggccgtgg ccgtggcggt caaaagcctt
     9781 gccgacccga cctgccagga catccgcgtg gtgcacgtcc ccagcggcga aattgttttc
     9841 cagactgcgc cgacgcacta aggaagcgct gaacaagtcc cctgcggcgc gcgatcagcc
     9901 gggctcgggc gg
//
LOCUS       DAIDFG010000091         9808 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_91_length_9808_cov_16.680406, whole genome shotgun sequence.
ACCESSION   DAIDFG010000091 DAIDFG010000000
VERSION     DAIDFG010000091.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 9808)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9808)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9808
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91_length_9808_cov_16.680406"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1645 a         2992 c         3269 g         1902 t
ORIGIN      
        1 cggtgctggt cgaaggcggt accgcaccgc agcggcccct gtccgtgccg gccgagttgc
       61 cgcgcgccgc ggcggcagac gctgtgcatg ccggcgccgc ggtgctggcc gcggaggctg
      121 gcagcccgcc tgcggcggcc aacggcagca gctgggaaac cgggctcgag gccgaggcca
      181 tggccttgct ggcgaccggg cgcagtgacg tgtgggatgt gctgacccgg cgtttcgagt
      241 ccaagctgat ccagacggcg ctgctcaaca cccgtggccg ccgcatcgaa gctgcgcaga
      301 agctgggcat aggccgcaac accatcacgc gcaagattca ggaactgaat ttagagtgaa
      361 tgtggcctcc acggtcacga tttctcatcc gatcgtcctg agcttgtcga aggatgcatc
      421 atccagcgtg gaccgtgttg ctctggttta aagtccagcc atgagccgct tatccctgac
      481 ccgcgacaag atctacaaaa ccgtggcgcg ccagctgcac ggccaggtgc cgtgctgggt
      541 ctgcggtgaa catgtgttgc cggcggccgc cacgctggaa cacatccggc ccctgagcga
      601 gggcggcaac agccacgcgg agaatctggc catcagccac gaggtctgca acggccagcg
      661 ccacgccaaa ggcgtgatcg ccgagtccgc cgaatcaccc gggcgggtgg cccgcaacta
      721 aagagtgaaa tcggccttca gcccaatcgg aacgggcgcg agcagctctg ttatttatag
      781 caaatcgaga tccgccacga ccggcacatg gtcgctgggc cgttcgtttt tgcgcggcag
      841 cttgtcgatc acgcagctct tcaccgcagg tttgagcgtg tcgctgacca ggatgtagtc
      901 aatacgcagg ccgcggttgc gccggaaggc gaactcgcgg tagtcccacc acgaataact
      961 tttttccggc tgctcgaaca ggcgaaacgc atcgtgcagg cccagtccca gcaacgcttt
     1021 caggtgttca cgctcttcgg tcgtgtggtg gatggtttcg cgcaggccct cggcgtcgta
     1081 ggtgtcgcgg tcgtccatcg tgatgttgaa gtcacccacc accaccagcc tggggtgggc
     1141 cgccagttca gccttgagcc agctgcgcag gccgtccagc cagcgcatct tgtactcgaa
     1201 cttgtcggtg cccggcgcct ggccgttgac gaaatagccg ttgaccagcc gcagttctcc
     1261 ctgcgacgtg tccagcgtcg cggcaatcac gcgcgactgc tcgtcgctga agccgttgat
     1321 gtttttgaca atatcgcgcg gtggcgtgcg gctcaggatg gccacgccgt tgtaggtctt
     1381 ctgcccgaag acggcgcatt ggtagccggc ggcagacaga gcctccagcg gaaacttgtc
     1441 gtcgctgagc ttgagttcct gcaggcacaa ggcatcgacc gggttggcgg ccagccagtc
     1501 gagcacctgc ggcagacgca ccgccagcga attgacattc caggtcgcga ttttcatgga
     1561 cgctccagcg tgacaaaact gtagggcagg ccactcgcgg ccacatggac atggcgggcg
     1621 atttcgcgcc agcctgggcc aagtgccggg gcatgggcat cgccgtcaaa atccctggca
     1681 atctcggtga cttcgatacg actggccagc ggctcggcct gcgcatatat ctgcgcaccg
     1741 cctatcaccc acacctcggg cgccgatccg gccagctgca aggcttcttc aaggctgccg
     1801 gcgcgctgcg cgccttcggc ctgccagtcc gcttgccgcg tgatcacgat gttggtccgt
     1861 cccggcagcg ggcggaattt aggcggcagc gagtcccagg tcttgcggcc catgaggacc
     1921 gggcagccct gggtcagctg cttgaagtgc gccaggtctt ccggcaaatg ccagggcatg
     1981 gtgccgtcct tgccgatgac gccgttggcc gcgcgggcgt agatgaggtt gatgcgggaa
     2041 gaggtcatga gttgtactgc ctgagccgtt cttcaagtgt tcctgtgtgc agctcaaaca
     2101 ggtggttgtc gaaatcgtaa aataaagcga ttggccttcc ccggggacgc gcggtcgctc
     2161 cggtctaacc tccgcgccta tggcgcgcag ccgggtttca aagccgggca gatcgcttgc
     2221 ttccaccttc aatgccaggt gccggtagct gcgctcggcc ggtggttcgc cctccatggc
     2281 tgccagccag gtgtcgccaa gcatgaaaaa tttttcccgt gacagcgaaa aattcctggc
     2341 cttgctgtcg tagacctctt tcgcacccag gccctcgcaa agaaacagcg ccatacgatc
     2401 aaggtctcgc acaatgaagg tgatgtggct gacaccctgg acaggcataa acgtccctac
     2461 accgccacag gcgccttgat cgccggatgg tgttcgtacc cgaggaactc gaagtcgtcg
     2521 aactcgtagt cgaagatcga tgcgggcttg cgcttgatgt tgagcgtcgg gtaggggaag
     2581 ggggcgcgac tcagctgcag tgccacctgg tcggcgtggt tgctgtagat gtggcagtcg
     2641 ccgcctgtcc agatgaagtc gccgacgtcc aggtcgcact gctgcgccac catgtgggtc
     2701 agcagggcgt agctggcgat gttgaaaggc acgcccagga aaatgtcggc gctgcgctgg
     2761 tagagctggc agctgagttt gcccttgccg cccggctcgg tggccggtgc cacgtaaaac
     2821 tggaaaaacg catggcaggg catcagcgcc atcctggaaa gatcggccac gttccaggcg
     2881 ctgacgatga tgcggcgcga gtcggggttg gttttcaggg tctggatgac ttcggcgatc
     2941 tggtcaatgt ggccaccatc aggggtgggc cagctgcgcc actgcacgcc atagaccggg
     3001 cccaggctgc cgtcttcgcg cgcccactcg tcccagatgg tgacgccgcg ctccttgagc
     3061 cagttgttgt cgctcgatcc ctgcagaaac cacagcagct cgtgcacgat ggacttgagg
     3121 tggaccttct tggtggtcac cagcgggaag ccttcgttga ggtcaaaacg catctggtag
     3181 ccaaacacgc ttctggtgcc ggtgccggtg cggtcggcct tgaacacgcc gtgggtgtcc
     3241 acatggcgca tgaaatgttc gtactgggag cggatagggc gaggtgacac gttaataagc
     3301 tccaaacagg cagtccatgg cggcaacgat gagttcactc tggtcttgca gaccggtcac
     3361 gcgctttttc aattcccgcg ccggaaacgc ggcgagggcg gcggtatcca gtaccaccgc
     3421 tttaccatcg acttcgaata cggggttcaa gtcacgcatg ggatgcgggt acagcgcggc
     3481 cggacgcatg gggatgacaa cgcgtgaatc cagactgttg atgtaatcgt tctggacatc
     3541 cagcagaaag ggcgcgatct tgcgcagcgc ggcatcgggg ttggcataaa catcgtaacg
     3601 agccatcgct agaaggtccg gtatttcgcc agcggcaggc cttcactggc aatgcgggcg
     3661 ttgtaagcgg cgacggcttc ccggttttcc tcgttccatt gctcccagta acggcgtcgc
     3721 acctcggcag ccagcaaggt atcaacggtc tgggaaacat tcatgcccat ctcgcgcgcc
     3781 atttccagca ccttggcgtt gagcgacagg ttggtggcct ttttggccgc gttgtcaaat
     3841 ttcagcatgg cgcatctcca tgagtcatgc gcatattata tgcgcagcac ccgccccggg
     3901 ttcatcaggt tctcggggtc cagcccgcgc ttgatggccc gcatcaggcc cagtgcgacc
     3961 ggcgatttgt gcttttcgag cttgtcgagt ttgaggctgc cgacgccgtg ttcggccgaa
     4021 atcgagccac cgaaggcctg caccgagtcg tagaccaggg cattgacgtt ttcttcctgc
     4081 tcgcgaagaa aggtcgccgc gtcgccttcg gccggagcct gcacgttgta gtgcaggttg
     4141 ccgtcgccga ggtggccgaa gttgacgagg cgcacgcccg ggatggcctt ggcgagcaac
     4201 tggtcggtga cgcgcacaaa ctcgggaatc agcgagaccg gcaccgagat gtcgtgtttg
     4261 atgttcaggc cttcctcggc ctgcgccagc gggatgcttt cgcgcacatg ccagagctgg
     4321 tgcgcctgct gcaggttttc cgcgaccacg gcatcggtca cgcaaccttc ttccatggca
     4381 gtttccagca ggcgctcgaa ctgctcgcgc gcatggcttt ccgactcgtg gtcggagttt
     4441 tccagcagca cgcaaaatgc cgtgtcttcg tacagcggca cgcgctgctg cttgaagtgt
     4501 ttggccacca ggccgagcgc gaactggccc atgacttcaa aacccgtcag gcccgcaccc
     4561 aggtggcggt gcgccagccc aagcagggtg acggcgtctt ccatcgacgg cacggccgcc
     4621 caggcggtga gctgcgcggc cggcatggga tagagcttca tggtagcggc cgtgatgatg
     4681 cccagcgtgc cttcgctgcc gatgaacagg tcgcgcaggt cgtagcctgt gttgtccttg
     4741 cgcaggcccg agaggccttc ccagacctcg ccctgcgcgg tgacaacttc cagcccgaga
     4801 cagaggtcgc gtgtattgcc gtagcgcacg acctgcgtgc cgccggcgtt gctgccgagg
     4861 ttgccaccaa tcgtgcagct gccttcagcg gccaggctga gcggaaacag gaagccggcc
     4921 ttttcggcgg tttcctgcag ggtctgcaaa atgcagccgg cttcgaccgt catggtcagg
     4981 ttggcgctgt cgatggcgcg caccgcgttc aggcgctgca ggctcagcac gacctggcgg
     5041 ccggaggcgt cgggcgttga gccgccgacc aggccggtgt tgccgccctg cggcaccatg
     5101 ggaacgccgg ccgcggcgca ggcgctgacg acggccgcca cctgcgcggt attggcgggg
     5161 cgaaccacgg ccagcgcctt gccgcgcgcg cgtttgcgcc agtccagctc ccaggccgag
     5221 aggtcgcccc cggtcagcac gtgggcgtcg ccgacgatgg cgcgcaaggc atcgagaatc
     5281 ttgtccatag gtttttcagg cagcaggttg aatggttgtt gcgccttttt tcaggcggat
     5341 gcgcacatgc agggcgcagg cggtgaacag cagcaggcac agcgcgactt cggccatggc
     5401 caggtagttg ccgggcggct tgtcgctcca ggcgcgcacc gcgccctcgg tgaaatacag
     5461 ccagaccatc aggctgagcc agcggtaggt gtacatgcgg tttttcagca ggccggccag
     5521 cggaaaacac agcggcagca ccttcagcgc cagcagcgag ccacccggtc gcagcggcgc
     5581 cagccacatc tcccaggcca ggccgagaat gatcagggcg gtcaggctgc ccacggccag
     5641 ccagcgggtg gcgtctatct gtgttgtttt gttgatggtc atgttgatgc ttgtgtcgat
     5701 ggcatgatac cggggatgaa attccagcag tccctgcagc agttattgac ggacctgtcc
     5761 cgcttcccct ggcgcgacac cgccatcacg ctgcgccagc gtttccgcga cgaccacatg
     5821 ggcctgacgg ccagcagcct gacctttacc accagcatcg cgctggtgcc gtttttcacg
     5881 gtggcgctgg ccatcttcac cgcctttccg atgttttcca agctgcaggg cgctctgcag
     5941 gtatggctgg tcaacagcct gattcccgac aacatctcgc gccaggtgct cggttacctg
     6001 acgcagtttg ccaaacaggc caacaagctc ggtgttgccg gcctggcggt gctgctggtc
     6061 acggcgatcg cgctgatcct gacgatagac cgcacgctca acggcatctg gcgtgtgaag
     6121 aagccgcggc cgctggcgca gcgcgtgctg atttactggg ccgccatcac gctgggcccg
     6181 ctggtgctgg gcgccagcct ggcggtgacg tcttatgtca tttccggctc gcgcggtctg
     6241 gtgggcaaca tgcccggcgg cgtgcgcgtg ttgctggacg tgctgcagtt tttcctgctc
     6301 gccggcggca tggcggccct gtaccgctat gtgcccaaca cctttgtacg ctggggccac
     6361 gcctgggccg gcggcctgtt tgtcgcggcg ggcatcgagc tggccaagaa ggtgctgacg
     6421 ctgtacctga gcagcgtgcc gacctattcg ctggtgtatg gcgcttttgc caccttgccg
     6481 attttgctgg tctggattta cgtggcctgg gtcatcgtgc tgatgggcgc ggtgattgcc
     6541 gcctacctgc ccagcctgct ggccggcgtg gcgcgccgcg gtagcgcgca cggctggcag
     6601 ttccagcttg cggtggaact gctgcagcaa ctgcaccggg cgcgcagcac cacgcgcatc
     6661 gggctggagg cttccgagct ggccagggcg ctgcaggtgg acgtgttgca gctcgaaccg
     6721 gtgctggaaa cgctgatggc gctggactgg ataggccagc tgaccgaccc ggaggacgaa
     6781 aacgaggcgc gttatctgct gctggccgat ccggcctcca ccgcgctgga gccgctgatg
     6841 gcgcagctgc tgctgagcca ggacccttcc atggaaaatt tatgggaaaa cgggcatttc
     6901 acggcgctgc gattgcgcga cgtgttatga aaatgatagc ggcccgggca tccatctgca
     6961 tgtggatgcc gtggctggtt gccatcagat cctgaccttg ccggtccaga tgtccttgta
     7021 catgacccag tcgcccatga agctgtagag cgggcgcttg aaggacgcgg gcttgttctt
     7081 ttcaaacacg aagtggccga cccaggcaaa gccgtagccg cacagcaggc cggccagcag
     7141 ccaccacggg ttgcgcgtgg cgatcattgt ggccaggcac agcagcgaga ggctggagcc
     7201 ggcgaagtgc aggcggcggc aggtgcggtt gctgtgctcg ctcaggtaga agggataaaa
     7261 ctcggcaaaa cttttgaagg ctttggcgtc tgccgtgggc ggtggcgtgc tggaagtggt
     7321 gctgtccatg gggtgtctcc ggaatagtcc ttgtttcgcc atgatagcca cgctcgcgca
     7381 caatggcggg catgcctcat tctttgcagt ttcctgccgg gcgctgggtc gcctgtcttt
     7441 gtgccgactg gtgcggcacc tgtcgggatt accggcccgt gtttgaccag gtggccgcgg
     7501 cgcaccccgg catgcgtttt ctctggctgg acatcgaaga agatgccgaa cgggtgggcg
     7561 aactcgacat cgagactttt ccgaccttgc tgattgccga tggcgcggcg cttcgcttcg
     7621 cggggccctt gttgccgcat gccggaacct tgtcgcgttt gctggcgtcg ctggaaaaca
     7681 aagccgtggc cgccggttcg gtcgatccgg cgctgacgga gctggtgcaa gcgctgcacc
     7741 gacagctcag ctcagcttga acccacggca aatggcgcgt gcagaaaacc cctgaaacgt
     7801 gcgcgcccgc cgcgggtcgg cgcagcggtc aggcggtagt cggggaaacg ctgcagaaag
     7861 cgcccgacgg cgatacgccc ctccaaccgc gccaggctca ggccggcgca ctggtggatg
     7921 ccgaaaccaa aggccagatg ccggttgttt tcgcgcgtca ggtccagctg ttcggggtgg
     7981 tcaaactgcg ccgggtcgcg gtttgccgca cctatgcaca gcgtgagcaa cgcgccttcg
     8041 ggcaggtcca cgccgccgac ctggcaggcc ttgagtgcgc ggcggttgcc cagctggttg
     8101 gacgactcga aacgcaggaa ctcgtcaatt tcaacagaca aatcggcctg cagccattgc
     8161 tgatcatgcg cgggcagctt cacttttgat agcagatgtg cccgggcgcc gggccactcc
     8221 tgcagggcga ccagggcgtt gccgatcagg ttggtcgtgg tttcatggcc ggcattgagg
     8281 atgaaaacgc agttttgcag cagctcggtt tcgctgagtt tttcaccgtt ctcctcgccc
     8341 tggatcaggc gcgtcagcac atcatgctcc gggtcgcccg gatttttccg gcgctcggcc
     8401 accagcgtct gcagataagc cgcgaactcg ctgacgctct ggttgcccag cgcttcctgt
     8461 tcgggtgtga gtttgggttc gagcgcgccc aggatggtca gcgaccagtc gcgcagcggg
     8521 ccgcggtcgg catgcggcac gcccagcagg ttgccgatga tctccaccgg aatcgccgag
     8581 gcaaagtcct cgatcaggtc gccgccgccc ctggcttcga tggcatcgag caggctgtcc
     8641 accagggtga ccaggcccgg ctccatgtcg gcaatggccc ggcgcgtgag ggcgcccatg
     8701 atgagcttgc gcacccgtgt gtgcagcggc gggtcgttga agaccaggct ggtcgtgtgg
     8761 tgctcgagca gcggcgcgtc cgcgccgtac ttgggcgtga actccacctt tttgtccgag
     8821 ctgaaggtct gcgcatcgcg gtagaccgcc acgaggtcgg cgtaacgcgt gaggaaatac
     8881 gagccgtccg gcatgcgctt gatgggttcg tgttcacgca accaggcata gacggggtag
     8941 gggttggcat agaagtcggg cggcagggcg cgcagatcga agctggcggc aatttcccgc
     9001 acacgttggg gcgacagggg cggtgtgatg gcgaagggcg tggcgtcggt catgcgccga
     9061 ttttggcatg cagacagccg caatgccggc ctgtttcagg ttgggtgcgt gggggtggcg
     9121 cgatgcgtgc gcggagcgcc accttcccgc gtcatgtcca tggggtgcgc gggcgatctc
     9181 gccggcgccg gacagcgtca ggcggctttt ttcgcccagg cgctgcacca gcccttggcc
     9241 gcgacttgct tgccggcgaa cagggggcag gctgcggccg cgtcggtcgc cttgccctgg
     9301 tacagcgcgc aattggcgca gttctggctg gcggcgtact tggggtattt cttggtgtcg
     9361 gtcttgctgc cgtcggcctt gtagcccagg gcgacggcat tggcgtcggt atccttgacc
     9421 atgggcgcgg ccgccgtctg ggacagcgcc aggctgctgg ccgccagcgc tccgctggcg
     9481 gtgagggcgt gaatgacaaa ggtgcggcgt gaagtggaag tcatggcaat cgtggaggta
     9541 gttgtgttgt cgtgcgtgat ggcactgcta ctctaccggc cggtcacgat cgggacttga
     9601 tggtaatcaa cagatgaacg cattccccgt gcattccccg gtgcctggtg cggcagcatg
     9661 gcgtggcggc tggtcgtgcc cttcatgtca cgaggtgcta gggcctgttc acactattta
     9721 tgcaagatgc gttgcgagtc aaaaaggcca tgcgcgaggc gcaaaatgca gccgggctgg
     9781 cgcccggcaa ggctttgcaa cgctgcgc
//
LOCUS       DAIDFG010000092         9704 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_92_length_9704_cov_20.213286, whole genome shotgun sequence.
ACCESSION   DAIDFG010000092 DAIDFG010000000
VERSION     DAIDFG010000092.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 9704)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9704)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9704
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92_length_9704_cov_20.213286"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1766 a         3153 c         3179 g         1606 t
ORIGIN      
        1 ggctccaaac ctgcctgcgc aggtttggac aggcgacaga agcgaagcct ctggcgagcg
       61 gcggcctgca aggtgcagag agctacacgc agtgagcgaa cgtggggggc cctatttcac
      121 ctcaacacac ccagcgcaaa cggcaggctg aacatgccga gcagggtcga caaggtgacc
      181 aggccggcca cataggcgcc gtcgtaaccc atgcgcgccg ccagcacgta acagctggag
      241 gcggtgggca gggccgaaaa cgccagcagc acggtggtct gcaccggcga caggcgaaac
      301 acccaggaca ggcccagggc gatcagcgga atgaagaggt ggcggatggc cagcagcgat
      361 accgccagga ttttggcttt ggccaaccgg ccgaactgca tgccggcgcc ggcagccatc
      421 aggcccagcg ccagtgaggc cgcaccgata cgggtcacgg tgggctccag ccagaccgga
      481 atctggaaac ccgccaggtt ggaggccagg ccggtggcgg tggcgatgat caggggattg
      541 cgcaccagcg caccgagaaa gccgctgttg gcgtggcgcg ccatgggcca gaccgcaccg
      601 acattgaaca gcggcacgca gacgccgatc agcacggcaa tcaggagcag gccttcggcg
      661 ccggccagcc gttcggccag cgccagcccg atgaaggagt tgaagcggaa agccacctgc
      721 gcactggccg cgtgctcccg cctgtcgatg tgcgccccaa gccagggcag gtgcggcagc
      781 gaataagcca gtgcaatacc gagcaccccc atgctgagcc cggccgcaat caggctggaa
      841 gccgcgccca ggtccagcgg gcttttgacg atggactgga acagcaggac cggaaacagg
      901 aagtaataca ccaggctttc cacctgctgc cacaccgtgc ggttgagggc ggtgtagcgg
      961 cacaccaggt agccgcagag aatgagggaa aaatccggaa agagaagttg cgcgtaattc
     1021 accccggcaa gaataaccgc aacaggcgca aagcctaggg accctctgcc tgactcgcgc
     1081 cgagcgcggc ggggcttttg cgggatgagc cgtgaggcgc aaaacccaag gcaaggctga
     1141 acgccttgcc tggttttgta acgaagcgga tcgcctgcaa aaccacgccc cttcgggttc
     1201 ggaccaaaac gggcgctttc ctcaccaaac cccttgcgtg tgcttcagca cacgctgcgt
     1261 ggtgttggcg agcaaatcat cccgttgtgg acccgaacgc gcatggcggg agtcaggcag
     1321 agggtcccta ggggattgcc cttatgcgta atccacaaca aggtggcacg gtgcttgcgc
     1381 ttacgatagg ggctgtccac tttctattgc aaggaaaatc gttatgcaac gtcgttatct
     1441 gcttaccctt tctgcactga cgttggccac ggccgcggca ggttccgccc tggcccagtc
     1501 ctacccgaac aaggtcgtca agctgcaggt gccttttgca ccgggcggca ccaccgacat
     1561 cgtggcccgt gtcatcagcg agccgttggg caaggccctt ggccaaaacg tgatcgtcga
     1621 gaacaaggcc ggcggcggcg gggtggtggg tgccctggaa accgcccgcg ccacaccgga
     1681 cggctattcg ctgggcatgg ccaccgtgtc caccacagcg gccaacccgg ccatcaaccc
     1741 gaaaatcccg tacaaccctg ccaccgactt cacgcccatc atcaacattg ccgccacgcc
     1801 caacatcatc gcggtgcacc cgagctttcc ggcgcgcaac tacaaggagt tcattgccga
     1861 actcaagaag aatccgggca agtactctta cgcctcgtcg ggcacgggcg gcattggcca
     1921 cctgcagacc gagctgttca agaacctgag cggcaccttc atcacccaca ttccttaccg
     1981 cggggctggg ccggccctga acgacaccgt ggccggccag gtgcagatca ttttcgacaa
     2041 catcccctcg gccctgcctt tcatccagca aaagcgcctg atccccatcg tggtcgccgc
     2101 gccccagcgt ctggcggtga tgcctgacgt gccgactttc aaggaagtgg gcctggagcc
     2161 tgtcaaccgc atggcgtatt acggcatcat gggccccaaa ggcctgccga aagaggtggt
     2221 cgacaaggtc agcgcggccg cgctcaaggc cttgcaggac ccggcggtgc gcaagcgcat
     2281 tgaagacacc ggctccatca tcgttggcaa cacgcccgag cagttcgcgg cgcagatgaa
     2341 ggccgagttc gaggtgtata aaaaggtcgt caccagcgcc gggctcaagc tcgactaacc
     2401 cggagcgggc cctgggctcg cgccgcaacg tacaccccgc aggctgcacg cctggcgggg
     2461 tgtttttttt gcgggctgcc cggcgggcgc aaccacttct ttcgcatttg ttatcgtcac
     2521 ggtatgaatc cccagagcca gtccggcatt gatgcctttg ttgacgcctt gtggctggaa
     2581 gacggcttgt ccaaaaacac gctggccgcc taccggcgcg acctgtcgct gtatgcgctc
     2641 tggctgggcg caaaagagca gggcggccgc gcactggatg aaacgctgga aagcgacatc
     2701 catggctatt tcgcggcgcg ccatagtgcc accaaggcca cctcggccaa ccgccggttg
     2761 acggtgttca aacgctattt ccactgggcc ctgcgtgagc gcctgatcac ggccgacccg
     2821 accctcaagt tgcagtcggc gaaacaggcc ctgcgtgtgc ccaagctcat gtcggaagcc
     2881 caggtcgagt tgctgctggc cgcgccggac atcgacacgc cgctgggcct gcgcgaccgt
     2941 gccatgctgg aactgatgta cgccagcggc ctgcgcgtga gcgagctggt cggactcaag
     3001 acctttcatg tcggcatgaa cgaaggggtg ctgcgcgtca tgggcaaggg cagcaaggag
     3061 cgcctggtgc cctttggcca ggttgcgcgg caggcgatcg aacactacct tgccgaagcg
     3121 cgccccgtga tcctgggcgg ccagcagacc gacgacctgt ttgtcaccgg ccggggcagc
     3181 ggcatgagcc gcgtgatgtt ctggatgctg gtcaagaaac atgcgttgcg ggccggcatc
     3241 acctcgccgc tgtcgccgca caccctgcgc cacgcctttg ccacccacct gctcaaccac
     3301 ggcgccgacc tgcgcgcggt gcagatgttg ctgggccacg ccgacatctc caccaccacc
     3361 atctacaccc atgtggcgcg cgaacggctg aaggcgctgc acgcgcacca tcaccccaga
     3421 ggctgattga gcccccacgc tcccccactt cgtgtgggtc gctgcccccc gagggggccg
     3481 ctgcgcctgc gggccggcga agccggaccc gcggcccctg cttgaacgga gagcctccac
     3541 ggtcgcttat tgagttcccg ttcgtcctga gcttgtcgaa ggatctcccc cgaaggacag
     3601 tgcttacccg gcgcggggca aaatgcttgc atgttcacct caggaaaatt catgtcattg
     3661 accccatcgc gccgccgcct gctgaccctg cctttgctcg ccctgtgcgc tgccgcgctt
     3721 ccttcgtttt caatcgccca ggccgcctgg ccggcacgcc ccattcgcat cgtcgtggcc
     3781 tacccgccgg gtggcctgag cgacaacatc gcgcgggcgc tggccgacaa gctggccgtg
     3841 cagctgggca cgccggtggt ggtggaaaac cgggccggtg caggcggcag catcggcatg
     3901 gacgttgtgg ccaaggccgc gccggacggc tacaccctcg gcttttcctc gatcagcccg
     3961 ctggcgctga acccgcacct gggcaagctg ccctatgacc cggcccggga cattgccccg
     4021 gtcgccagcg tgatgtattc gccggtggtg ctgctggcca cttctgcgtt tgagggcaag
     4081 gacttcaagg acctgctggg agcggccaag gccaaacccc gcagcgtgcg ctggtccacc
     4141 tcgggcctgg ccacggtcgg ccacatcgtg ctcgagcagg tccagcacgg tgccaaagtg
     4201 gagatcaccc acatccccta caaaggcggc ggccagcaac tgaccgacgc cctgagcggg
     4261 cagtttgaag tcatcaccac caacctcagc ggcgcggtga tgccgcacat ccagtccggc
     4321 aagctgcggc cgctggccgt cggtgcgccg gcgcgggtgg aggccctgcc gcaggtgccg
     4381 acctttgccg agctcggttt tgcgccggcc aacctgagct cgctgttcgg cgtgtttgca
     4441 cccggaaaaa cacccgaggc cgtgctgacg cgcctgaaca ccgaaatcaa tctggccctg
     4501 aagacgcccg agctgcgcaa gcgcctgctg ggcgccgaca acgtgccgac gggtggcagc
     4561 gccgccgagt tcggccggca gattgcgcag gagtcggaca acaacgcccg catcatcaag
     4621 gcggcgcaga tcaaggtcga ctgaagcccg gggtgtggag tgctattgat tcaatagctg
     4681 cctgcgaccg ccacataagg gctggaggct gattttattg ataattttcg gaaggcgacc
     4741 ggttcaaccc ggtgcgctgt ccgcgccggc agtcggcgca aacatgtagt cgcgccggaa
     4801 ggggtagcgg ctttgcgcgc ccttgatggc ttcgtctgcc atggagtcag ccgggcgggt
     4861 ggccagcatc tggtgcagcg agagccagcc ctgctcgaaa ctcatggcgc tgccggccag
     4921 gtagaggcgg taggcgcgca ggattttttc gccgcgctcg ctgccgccgc tgcccgtggc
     4981 cagcacctgc tgcgcggcgt ccagccgggc ttccagcgca tcggaccagt cccacaaggt
     5041 gcgcgcatag tggggccgca ggttttccgt atcgaccatc tccagcccgc cgcgcgccat
     5101 gtggttgagc acgtcgctga cgtgcagcaa ttgcccgccg gggaaaatgt atttctcgat
     5161 gaagttgccc atgcccgcgc cgagctggtc gttgtccggc ccgccggcgg tgatgccgtg
     5221 gttcatgacc agcccgcccg gtgccagcag gcgaccgatc ttgctgaaat aagccgtcat
     5281 gttggcgcgg cccacatgtt cgaacatgcc aaccgacgag atcttgtcga agggctggct
     5341 ttcatccagg tggcggtagt cgcgcaacgc catgcggacc cgcccctgca ggcccttgtc
     5401 ttcaatgatt ttctgcacat gggcatgctg gtttttcgac agcgtgatgc cggtggcatc
     5461 cacaccgtaa tgttcggcgg cccacagcag caggccaccc cagcccgcgc ctatgtcgag
     5521 gaaacgctca cccggctgca gcatcaactt gcggcagatg tggtcgagct tggcttcctg
     5581 cgcctgggcc agggtcatgg cgtcgtcccg gtagtaggcg caggaataaa cgcggcgcgg
     5641 gtccagccac agggcgtaga agtcgtcaga aacatcgtag tgaaactgga tctgctcggc
     5701 gtctttttcc ggcgtatgcg cccggagtgc ccggatctgg cgctgcaggg tgttccacca
     5761 gccggcgtcg atgtggaccg ggttggccgg cagcaggctg gcggccacgg ccatcacgtc
     5821 gcgcatgcgg ccttccagat gcagcttgcc ctcgacaaac tcctcggcca gcgcgccgat
     5881 ctggccggcg cgcagccggg tcaggctgga ccagtgggaa aacgacatct tgaccggcgc
     5941 gccgggcggg ccgagcacct ggcccgcggg caactgcacg gccatcggcg acggcaggcg
     6001 cacgagttgc gactcaatct ggggaacgag atgccggatc attgcaaacc ctcctcggag
     6061 acttgacggt ctgaagctga aatattactc aatcctgcgc cagcttgccg gtgtcccgtg
     6121 ccctcgcgcc gtgggaaaaa ttcccgattc agtgtcagcc gccccggagc ctggggtatt
     6181 gacatgtatc aagctggcgg gcttgcgtgc cacgcgcggc tgccgtttcc cggttcaggt
     6241 ccccagccgg gccagctcgg cctcgtcaaa cccggcggcg cgccgggcgg ccaggttgaa
     6301 cgggcctctc agcgcgggcg cttcatacct ggcggcaagt tcagcataag tcgccacggg
     6361 atcgagttgg cgctgcccgc acagatgccg gtaccaatgg ttgccgatgg cgacatggcc
     6421 gatctcgtcg cgcaggatga tgtccagaat cgccaccgcg cgcagggcgg cgggactgcc
     6481 gacccggcgc agtttcgcct gcatcgcggg ggtggcgtcc agcccccggg cttccagcgt
     6541 gcgcggcacc agcgccatgc gcgcgagcac gtcgccggcg gttttttcgg tcatgtccca
     6601 caggccggtg tgggcgggaa agtcaccgta gtcataaccg agggtctgca ggtgctcgcg
     6661 cagcaggctg aagtgcaagg cttcttccgc ggcgactttc agccagtcca ggtaataggc
     6721 gggcggcatg ccgtcaaagc gccaggcggc gtcgagtgcc aggttgatcg cgttgaattc
     6781 gatgtgggtc accgcgtgca gcagggcggc cagcccctgc ggggtgaagg gagaacgctt
     6841 gggcacatcg aggtgggagc gcagctcggg ccgggccggg cagccgggca ggccggtggg
     6901 cgcctcgggt gggtcttgat acgctatcga aagagtagct gcctgcgccc acagggattg
     6961 ggccagcagc actttctgct gtggatcggg ttccatcagc gcctgcaggg cctgttggcg
     7021 cagttcgggg ggtgcgggtg tcgccgtcat ggctggattg tcggcgatag cgcgcaacag
     7081 gcgggcagcg ccgggcaaag acagggtgcg gatgcctgcc tacaattggg gctcttcccg
     7141 ccgcgccctg ccgcgccagc ggcccctttc ccccgacttc acaaagaacc atggccctct
     7201 accaactcga cgacctgacc cctgccgttc atgaatccgc ctgggtcgcc gacagtgcgc
     7261 aggtcatggg caatgtgtcg ctggccgagg acagcagtgt ctggttcggc gtggtgattc
     7321 gcggcgacac tgaaaccatc cgcgtgggca agggctccaa catccaggac aacagcgtcc
     7381 tgcatgccga tatgggcatg ccgctgacta tcggcgacaa cgtcaccgtg ggccaccagg
     7441 tcatgctgca cggctgcacc atcggcgacg gttcgctggt cggcatccag gccgtggtgc
     7501 tcaatggcgc aaaaatcggc aagaactgcc tggtgggcgc cggttcgctg gtgaccgaag
     7561 gcaaggagtt tcccgacggc tccatgattc tcggcagccc ggccaaggcc gtgcggcaac
     7621 tgagccccga acagatcgag ggtttaaaaa tgagcgcgca gcactatgta gagaatgcgc
     7681 ggcgttacag agccggcttc aaaaagattg attgatgtct gaattacaca agtttatttt
     7741 cgacggcctg ccggtgcgcg gcatgctggt gcgcctgacg gacgcctggc aggaaatcct
     7801 caaacggcgc gaggccggcg gcggttaccc gcccgaagtc atgcagctgc tcggtgaaat
     7861 gacggccgcc ggggcgctga tgcaaggcaa catcaagttc aatggggcgc tggtcctgca
     7921 gatcttcggg gacggcccgg tcaagctggc cgtggctgaa gtgcagcccg acctgagcct
     7981 gcgcgccacc gccaccgtga ccggtgaggt ggtgccgggc gacaccttga gccgtctcgt
     8041 caacgtcaac aaccagggcc gctgcgcgat caccttggac ccgaaagacc ggctgccggg
     8101 ccagcagccc taccagggcg tggtgccgct gtttggcgac cagcatgaaa agctcgaaaa
     8161 cctcagcgag gtgctggagc actacatgct gcaaagcgag cagctcgaca cccggctgat
     8221 tctggcggcc gacggccagg tcgctgccgg cttgctgatc cagcgcctgc ccgtgcaggg
     8281 gcagggcaac ctgcagggcg gccagcgcgc cgacgaagac gagatcggcc gcaacgaaga
     8341 ctacaaacgc attgccatgc tggccggcac gctcaagcgc gaggagttgc tgggcctgga
     8401 cgccgacacc atcctgcacc gcctgttctg ggaagaaaaa gtcacgcgtt ttgtgcccag
     8461 cacgccgacc tttgcctgca gctgctcgca ggagcgtgtc agcggcatgc tccgcagcct
     8521 gggcgcggcc gaggtggaaa gcatcatcgc cgagcagggg cgcatcgacg tgggctgcga
     8581 gttctgcggc gcgcagtacg gtttcgatcc ggtcgatgcc gcgcagattt tcacccgcca
     8641 ggaaagccag ctgccgcccg cgcctaccgt gcagtagttg ccgtgcctgc tgccgtcagc
     8701 gcaaccggcg ttccagcagg tcgctgagca ggcggcgttc gcaacccggg tcgctgcagg
     8761 tgtcgcaggc ccagacccag ggcttgcgct cccggctatc gtccgcaagc ggatgcggct
     8821 tgccgccgtc cagcagcccc tgcagcgcgc ccagcgcatg ggccaggcgc tgcggcccct
     8881 ggccgtaaac cacggtgtag ggcagctccg cgttggcaag cgtcaggcgc agctgctggt
     8941 cgaccgcctc ctgcgctgcc gcgagaccgg ctggcaccgg cagatccaga cccatgagca
     9001 ggacctgatc gaaaccgaag ccacgtgggc cggtcggtgc gccggcgcgg gcctgccgaa
     9061 cagtcgccag cggcgggttg tcggcaatca ggatggaaag gagggccggg tgcggggagg
     9121 ggcggctgtc cagcgccgcc ctgagttcgc cagccagccg ggtcttgccg gtacctgctg
     9181 cgcccagcaa ggcgatgtgg cgcgaagcag gcccgtgggc aagcggggcg gtcaaataat
     9241 gagccaaatc aggctacagc ccttataggt aaagcgccgg cagctatcaa aagcagagca
     9301 aaagggaggc gagcgagcgg gctgcggtca gcgcgcaatc tgcacaaaaa tttcattggg
     9361 cttgaccatg cccagctcct ggcgcgcttt ttcctcgacc atctccagtc cttcccgcag
     9421 gtccttgacc tcggcggcca gccgttcgtt gtcggcctgt gcctgggcgt ttttgacggt
     9481 ctgctcgctg agcctgcgct gcatctcgtt gacgctggtc aggctgcccc ggccaatcca
     9541 gagttgcgca tgcacgatga ccagcagtgc aatcaggagg gcggggacaa cgcggttacc
     9601 cacgatcaac taagggtgtt cagcgcaagt tgtaaaaagc gtcgcggcca ggataagtcg
     9661 cgatgtcacc caggtcttct tcgatgcgca gcagctggtt gtac
//
LOCUS       DAIDFG010000093         9663 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_93_length_9663_cov_17.987510, whole genome shotgun sequence.
ACCESSION   DAIDFG010000093 DAIDFG010000000
VERSION     DAIDFG010000093.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 9663)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9663)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9663
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93_length_9663_cov_17.987510"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1642 a         3231 c         3168 g         1622 t
ORIGIN      
        1 gcagaatcgg gccccagccc aatcaccacc tgcgccacca gctatcaaaa cagtagtggc
       61 ggccggccca cccaccggcc ctcaccgcgg tggcggcagc ggctcgcccc acatgcgccg
      121 ccacagccag ggcagggccg tcgccacgtc gggccgcgcg cttttgaggg tgggtacatg
      181 gctttgcgcg gcgcgagcgg cggcggtgcc caggtcgaag tggaaggtgt aggccggctc
      241 ctggcccatg cgcaggtcgg tgacaaacag gcgcccgccc gcttccgaca ggcggaaaaa
      301 cccgtggctg aaggccgcga tgcgcgccac cggcgggtcc tgaccgtgca gcgtgatcag
      361 ctcggcgccg cggtcgtgcg cggtccaggt gatgcgcggt gatacgtcga gcagcgagta
      421 gtggccttca aaatactgcg tgggggtggt agccaccagg cgccacagca gcgtgttgaa
      481 gggcgcgggc gtcaccagca gcgcattggc ggtgatgccc tcggcctgca gcgagctgcg
      541 cgccaccccg gtcgcctgct gctgcgccag cacgctccag cccaggtaaa gcgtgctcag
      601 caccagcgcc gccgcgttcc agcgcaggcc gcgtgcgctt tggagccgca gcgcggccac
      661 cacgccgacg atcagcggca cggtgtaaag cgggtcgatg atgaacacgc tgcccacgcc
      721 gaacgggtag ttggtgaacg gcaacagcag ctgggtgccg tacaccgtca tggcgtcgag
      781 cagggggtgg gtgatgagca ccagccagag cgccagccac cagcgcttga acagcgcgcc
      841 ctcgccgtgc aggcgcgaga ccccccaggc cagcggcggc gcggccagcg tgaggtagaa
      901 cagcgcatgg ctttcggcgc ggtgcagcgt catgttgagg atggcgttgc cgtggtcgat
      961 gaaggcgtcc atgtccggca aggtgccggc caccgcgccc cacagcgcgg ccttccacac
     1021 ggcggtgcgc cggcccatga cggccacgct cacggccgaa cccagtgcaa tttgcgtcaa
     1081 tgaatccatg gcgggcagct taacgcagcg gcccgctggc aggcgcagcg tgtggccctt
     1141 gccccctgac cgggccgtgg cgttgggcgc gcagcggacg actgccccgt gcgcgtacgc
     1201 cccgttacaa tctcgctgcc tgatcgccgg catgtgacca ccggcggcct ctggtgcggc
     1261 atcacggtgc ggccagaaat gtgtcaccgc ctaccgggtt ccaaccatga cgtccatcaa
     1321 gaccctgctc gtgctctcca ccaccttcgc gctgctggcc tgcagctcca tgggcagcac
     1381 gcccatgagc agcgacagca cggtgaccga ggccacgtcc aaacaactgg cgggggacgt
     1441 gccgctgccg cagggtgcgg tcatcaagca gcaggacaca ctggtcatgg gctcgggcaa
     1501 cacctggatg gggcggctga gcctgacggt gccgggcgag gcgcaaccgg cgtttgcctg
     1561 gttccgcgac aacctgcccg ccgccggctg gacgctgacc tcgagttcgt tttccaaact
     1621 cagcctgctg accttcacca aggcagaacg tgtggccacg gtgcagatgc agggcagcaa
     1681 ctttggcggc aacgaggtgc tgatcacggt ggcgccggcg gtgcggccgg ccagtcggcc
     1741 ataagctggc cctacgccgg ccgtcaaccg gactgctatt gaatcaatag cttcctgcgc
     1801 ccgtcctgct tgggctggag tccgaaaacg ctgtaaaaat ttaactactc agatggtctg
     1861 ggctggtttt gctccctctc cctctgggag agggcggggg tgagggatgg cggtctgtgc
     1921 agggaggtat cgtgggttgt ggcttgctgg ccctcacccc aaccctctcc caaagggcga
     1981 ggggacaaga aaagcgtgct ccagccagcg acttgagtcg ttacgtaaaa ataaggcctg
     2041 cggcccgata actctttaaa gcaggctctt tcaggccgtc tcatccggct ggccggcaat
     2101 ctggctgacc agcaccttgt cgatgcgttt gccgtccagg tccagcactt caaaacgcca
     2161 gcccgcggcg tccaccagat cgccggtgcc cagcaggcgg cctgacacgc tttgcagcag
     2221 gccggccacg gtgttgtagc ggcccctgtc ctcgtcgggc agctcccgga tgtcgagcct
     2281 ggccttgagc tcggacaccg gcatcacgcc gtcaatcagc cagctgccgt cggcgcggcg
     2341 tgtggcccag gcatccacct gcgcgccggg ctgcagctcg ccggtgatgg cttcgagcat
     2401 gtcgatcggg gtcatcaggc cctgcaccac gccatattcg tcgaccacaa acaccatgcg
     2461 ggtggactgg gtgcgaaact gctccagcag ttccatgccg gtcagcgttt caggcacaaa
     2521 catggccgga atcgcgtagg tgtcgatgcg gtcggccagt gtcggtgtga tgccggtacc
     2581 ggtttcctcg ccgaccggct ggatctggcc gcgcagggcc agcagcttgg caatgttcac
     2641 cacacccacc acgtcgtcga ggctgccgcg gcacaccggg taccacgagt ggccggtggc
     2701 gccggccttg acaatcgcct gcgccacggt gtcgctggca tccagccact cgatgtccga
     2761 acgcggccgc atcagcgagg tgagcagccg gtcgtcgagc agaaaaacgt tctgcaccat
     2821 ctggtgctcg tgctcctcga tcagcccggc atcgacgccc tcttccaggc tggcggcaat
     2881 ttcctcgtcg gtcacggtgc gctcgccggc gttgtccacc cgcagcagct tgagcacgct
     2941 gtgggtgctg aaggacagca gccgcacaaa aggcttggcg ccggtggcca cccacatcat
     3001 ggggcgcgac accagccgcg ccacggtttc ggggtaaagc tggccgatgc gtttgggcac
     3061 cagttcgcca aacacaatgg tgatgaaggt gatgacggtg accaccagcg cggtggccgt
     3121 gatgtcggcc gcgcgcgtgg aggcgccgaa ggtctggatc cagcttgaca ggctgccgct
     3181 gaaggccgcc tcgccgatga tgccgttgag catgccgatg gaggtgatgc cgacctgtac
     3241 cgacgagaga aactgcgtcg ggttctccag cagtgtcagc gccgcccccg cacccttgtc
     3301 gcccgactcc gccatggcgt tgagcctggc cttgcggctg gaagcgagcg ccagctcgga
     3361 catggcaaaa acgccgttga gcagggtcaa aaaggcgatg aggatgaaat ccatggatcc
     3421 agaggcagaa tgaacacatg gcactttact tgattggcga cctgcagggt tgcaacgagc
     3481 cgctgcagcg cctgctgcgc agcgtggatt tttcccccag ccgcgacacg ctgtacctgc
     3541 tgggcgacct ggtcaaccgc ggcccggact cgctgggcgt gttgcgcagc ctgtccgccc
     3601 tgggtgatgc ggcgcagtgc ctgctgggca accacgacct gaacctgctc ggcatccgcc
     3661 acgggctgcg caagccgcac cgcagcgaca cggtgcagca ggtgctggac gcacccgact
     3721 gcgagcccct gcttgactgg ctgcggcacc gccgcatggc catgctggaa cacggctggc
     3781 tcatggtgca tgccggcgta ctgccgcagt ggagcgccgc gcaaaccatg gcgctggccg
     3841 gcgaggtcga gcaggcactg cgcggccccg atctggcggg atttctgaaa gcaatgtacg
     3901 gcaacacacc ggaccagtgg caggacacgc tggccggcgc cgagcggctg cgcgtgattg
     3961 tcaatgccct gacacgcatg cgtttttgta ccgccgacgg cgccatggaa tttgccacca
     4021 ccggcagcac gcagacggcg ctccccggtt acctgccgtg gtttgaagtg ccgggccgga
     4081 aaacggccgg tctgcccatc gccttcggcc actggtccac gctgggcgcc gagaccgtgg
     4141 ccagcgggtc cggcctgctg gccaacacct tgccgctgga cacgggctgt gtctggggcg
     4201 gctgcctgac ggcggcgcgg ctgggcagca agcccggtca gttcgagttg atccgtgtgc
     4261 aatgtccgca ggtccaggca ccgggctgag tgctgccgga aattcaagga aatccaagca
     4321 tttcaggctt ccagcccaat gactgcggac gccatctgct atcaaaaaag cagcaaacca
     4381 gcggcctacc gggcactcag gcgggggccg ggccggcttt cctgagcgcc gcctcgtcgg
     4441 acagggcgcg cgccacactg gccgagagca gcagcacggc ggacgagaag tagatccaca
     4501 tcagtagcac caccagcgag ccggccgcgc cgtaggcaga caccacggcc gcggtggaca
     4561 ggtaaatcgc catcaggtac ttgcccagcg caaacagaat gccgccggcg atgccgccca
     4621 tcaccaagta acgcatggca ggtttgggcc cggcgctcat gcgcatcagc gcgacgaaca
     4681 gccccgcgga aaacgccatg gacaccagct cgttgatcac caccgccacg cgctccagcg
     4741 gcatgtagct gccggcccag gtggtcagga aactcagcac agcggagatc accagcgaca
     4801 ccagcaacag aaaaccaaag gccagaatgt aggcaatgcc gcgcacgcgc agcgacgcgc
     4861 cgtaccacca cttctgctgc ggcggcacgc cggtgtcctg gcgccagagc cgctcgaagg
     4921 cgtcctgcag ttcggcaaac acgccggtgg cacccgacaa cagcaacaca aacgcgatgc
     4981 ccgatgccag caacccctcc gacggctcct gcgcgctggc cagcgcctgc ttgatcatct
     5041 gcgcgccctg ctcgccgatc acgccgccaa tctcccggat caggctgctt tcgatgtagc
     5101 tgcggtccag ccaccagccc agcacggcca ccagcagcac cagcaggggc gcgaggctga
     5161 gaatgccgta gaaggacatg gcggcactca tgcgcaggcc gtccgcgttc atccacaggt
     5221 cgacagcgcg gcctaccggg cggaacaggg tgggaaagga agagcgctgg taggcgcggc
     5281 gcaggccagc gtaggagggc atcatggccc cagcttagcc cggctgacgg tgcaaaacca
     5341 tcggacgcct gcgcgggaaa cagtaggccc cggctgactg ccggcggcct cctgctgcct
     5401 cagctgcctg tcttgaacag cgcagcgact tttttcttag gcttgatgtt ggccgacacg
     5461 ccgcgtgcgg cgccgccctt ggcggccgct tcccaggccg gctgcgcttc ggccggcagg
     5521 ctggattcgt aaggcttgtc aaagaagggg tcgcgcgagg ccgactggcg ttgcggcggt
     5581 gcctcgcggc gttcccggcg ttccggcctg tcgcgctggg ccccggcctc ctcgcgcacg
     5641 ggctggtcgc cgctgtcgcg gtacatgcgc cggccgtcgt tgatgcggcc ctgctccttg
     5701 atgcgcggct ggtcttcctc gaactccatg ggttcgagct cgatcttggt cttgatgagt
     5761 ttttcgatat cggtgaccag gcgctggtcg ctggcggccg caaaactcac cgccaggccg
     5821 gaggcgccgg cacggccggt gcggccgatg cggtgcacat agtcttccgc gttgaaaggc
     5881 acgtcgaagt tgaacaccgc cggcacgtcc ttgatgtcca ggccgcgcgc ggccacatcg
     5941 gtacacacca gcaaatcgac ttcgccgctt ttgaaggcct cgagcgcttt gagccgctcg
     6001 tcctggcttt tgtcaccgtg cagtgcggtg gtcttcaggc cttcgcgctc cagcgagcgg
     6061 gccaggcgcg cacaaccgag cttgctgttg acaaacacaa aggcctgctt gatgccgcgg
     6121 gtcttgagca cctggtgcag cgcgcggcgc ttgtcgtcgg cactgacggc gtagaaatgc
     6181 tgctccaccg tggacgccgt ggcgttggaa cgcgccacct cgatggtcac cgggtcctgc
     6241 aggaagctgg atgccaggcg cttgatctcg ggcgagaagg tggcggagaa cagcagggtg
     6301 atgcgttgct tgggcaggta gctcaggatg cgctgcaggt cgggcagaaa gccgatgtcc
     6361 agcatgcggt cggcctcatc gagcaccacg tactcgacct ggttgagcac ggtgttcttg
     6421 gcttcgatgt ggtcgagcag gcggcccggc gtggcgacca gcacctccac gcctttcttc
     6481 agctcggcgg tttgcggctt catgtccatg ccgccgaaca ccaccgcgct gttcaggttg
     6541 gtgtacttgg cgtagaactt cacctgctcg gcgacctgca ccgccagttc gcgcgtgggc
     6601 agcagcacca gcgcacgcac cggatggcgc gcgggcgagg tcgaagcgtt ctcgtgtttc
     6661 atcatgcgct gcagcagcgg caaggcgaaa gcggcggttt tgccggtgcc ggtctgggcg
     6721 gcgcccatca cgtccctgcc ctggagaacg accggaatgg cctgctcctg gatgggggtc
     6781 atggtttcgt agcccatttc ggctacggcg cgggccagcg gttctgccag cgaaaggttc
     6841 gaaaaagatg atgtcattta gcctgctatt gtcgcacttt gggtgacaaa ggcatttcag
     6901 ccggcccgcc gcccggtcgg gcctgcggat gaccgggctc ccgtcgcctg gatgctattc
     6961 atttaatagc tgcttgcgca tacaggacgt gggctgaaag ccttttttct ataaattccg
     7021 ggcggagaag gtgtcgcacg ccttgacatt gccctgctgc aagccgttgt tgaaccagcg
     7081 cgcacgctgg gcgctgctgc catgggtaaa actctcgggc acgatggcgc cgccgtttcc
     7141 gcgctgcaac gcgtcgtcgc cgatcttcgc ggcggcattc atggcctcct cgacgtcgcc
     7201 ctgctccagg atggcacggg cgcgctgtgc gtggtgcgcc cacaagccgg caaaacagtc
     7261 ggcctgcaac tccagccgca cgctcatggc gttgtattcg gtcttgctga cacggccgcg
     7321 catctggtcc atcttgccgg tgatgcccag ctggttctgc acatggtggc cgacctcgtg
     7381 cgcaatcaca taggcctgcg caaagtcgcc gggcgcgccc agctttttct gcagggtttc
     7441 ataaaagccg aggtcgatgt agaccttctg gtcggccggg caataaaacg gccccatggc
     7501 cgcctgcccc tggccgcagg ccgtggcggt ggcgccgcga aacagcacca gccgcggctc
     7561 ctggtaggtg ctcccgccct gggtgaacac cactttccag acgtcttcgg tgtcggccag
     7621 cacggtggat acaaaactgg ccatgcggtc atccgccggc ggacgctggg cggggccctg
     7681 ctgcacctgc gccgtcgggg tgccgccgcc gctcagcagg ctcaaaatgg tcagcgggtt
     7741 gatgccgaaa atccagccgc ccaccagcgc gatcacaatc gtgccgatgc cgatgctccg
     7801 gccgcccagc aacccgcccc ctccgcctcc actgcggcgg tcctcgacgt tgtcggattc
     7861 gcggttgcct tcccatttca tgattgactt tcgttggcgt cggcgccgca gaggcggcgc
     7921 aaactgtgcg gattacttta cttgagcttt caattcagag acggaacatt ttgttcgtcg
     7981 cagcatccca ttgaacatga acagccttct tgcacgcgcg cgtctttttc ccgtcctgct
     8041 gtcagcctta ttgctgagcg cctgcgcgag cccgctggtg acacaggtca ccagcttcaa
     8101 ccagtggccc actgacgctg ccggcgccag cttcagcttc atcaggccgc cacccaaccg
     8161 cctgggcgcg ccgcaggagc tggagcaggc cacctatgaa cggtatgcgc agctggaact
     8221 ggaaaaactg gggctgcggc ctgccgccgc gggccagcag gcgcgcctgc tggccgagct
     8281 gagcacggtc aaccagaccc agcaacggac ctatctgcag ccggtgtatg aagaccccct
     8341 ggtgttttac ccgccgcgcc gcaacgccgc cggccagtat ttcccgggct tctgggcgcc
     8401 cagccgcttc ggtccgacct atgtgggcga ccgggtcgtg ccctacaccg tgcagttcag
     8461 ccagctcaac ctgcgtcttc tcgatacccg cagcggcccg ccggggcagc cccgcgccgt
     8521 gttcgaatcc cgcgcggtgt atgaaggcag cgccagcctg cctgcggtca tgccttatct
     8581 ggtgcgcgcc gcgctggacg gctttcccgg ccagaacggg caggtgcgcc aggtgcgttt
     8641 cgaccgggaa accggcgcac tgatcagaaa ataagcgcct tgcacggccc cgggatcgac
     8701 gctgccgagc gcagggtccg gcctgcttca caatggtggc atgccacgcc cgacgacctc
     8761 cccgcccctg ccacgcatcc tgctgaccgg ttttgacgcc ttcggcggcg acaccctgaa
     8821 tccgagctgg ctggcggtcc aggcgctgca cggccggcgc atcagcggcc atcaggtggt
     8881 ggccgcccaa ctgccgaccg tgtttgaggc ctcgctggac gaactgcggc gattgctgcg
     8941 cacgcacaag ccggcgctgg tcatttgtgt cggtcaggcc ggcgggcgcg ccgccctctc
     9001 gctggagcgt gtggccatca atgtgaacga cgcacgcatt gccgacaatg ccgccgccca
     9061 gccggtggac acgcccgtcg tcgccggcgg gccggccgcg tatttcagca cgctgcccat
     9121 caaggccatg ctgaaggcgc tgcaaaaaca aggcattgcc gccgaggtct cgcagaccgc
     9181 cggcaccttt gtctgcaacc atgtgtttta cggactcatg cacatgctgg ccacagggcg
     9241 cggcttcaag cgcacgcgcg gcggctttgt gcatgttccc tttctgcccg gccagggcca
     9301 gccccacatg gcgctggagg acgtggtgcg cgggctgcgt gtggccgtgc gcacggcaat
     9361 cacaacgcag cgcgacatca cgcagggcgc gggagcgatc agttgatttt ttggggagcg
     9421 ggctcgtccc gcagaagccg ccagaccagc caactgccga tcagcgcgtt gctgagggcg
     9481 aaaccgccga cgatcagggt ggcccaggtg tccagcaccc ggttgtgaac aatccaggcc
     9541 agcgcggccg acagggcatt gagcgccatg gccatccaga acaggggatt gcggggctga
     9601 aacaggcggg ataacttcat gcagggaatg ctaaccgctg ccggcgcccc ttcctggaac
     9661 cgc
//
LOCUS       DAIDFG010000094         9627 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_94_length_9627_cov_18.021208, whole genome shotgun sequence.
ACCESSION   DAIDFG010000094 DAIDFG010000000
VERSION     DAIDFG010000094.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 9627)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9627)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9627
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94_length_9627_cov_18.021208"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1634 a         3078 c         3264 g         1651 t
ORIGIN      
        1 cgcaatgcgc gcaccgtgga aatgcaggac ttcgaaaaag ccaaggacaa gatcctgatg
       61 ggccccgagc gcaagagcat ggtcatgccc gagtcggagc gcaggaacac ggcctaccac
      121 gagtcgggcc acgccctgct cggcaagctg ctgcccaagt gcgacccggt gcacaaggtc
      181 accatcattc cgcgcggccg cgcgctgggc gtcaccatga gcctgccggc ccaggaccgc
      241 tacagctacg accgcgagta catgctcagc cagatcagca tgctgtttgg cggccggatt
      301 gccgaggaag tgttcatgca ccagatgacc accggggcca gcaacgactt tgaacgcgcc
      361 acggcactgg ccagggacat ggtcacgcgt tacggcatga gcgaggcgct gggccccatg
      421 gtttacgccg agaacgaagg cgaagtgttc ctgggccgtt cggtcaccaa aaccaacaac
      481 atgagcgagt cgaccctgaa aaaggtcgac gacgaggtgc gccgcatcat tgaccagcag
      541 tacgcccagg cgcgcacgct gatcgaggac aaccaggaca agattcacgc gatggccaag
      601 gccttgctgg agtgggagac cattgacgtc gaacagctcg acgacatcat ggccggcaag
      661 gagccgcgtc caccgaaaga ctggacaccg cgcaacccgt cggtgggcgg tagcggtggc
      721 cccagcggcg gtacgccggc ggtcacgacc gacccggcac ccacggtggc ctgagcgcct
      781 ccatccacat gacaaccggg gcaagtcccc ggtttttctt tggctcgaca ataccccatg
      841 atctggcaaa cctcgcgctt tcaaatcgac ctgagccagc cccgcgtgat gggcattgtc
      901 aacgtcacac ctgattcatt ctcggacggc ggcaaatatt tctcggtgga cggcgcgtcg
      961 cgcgccgcat ttgcgcattg cgagcagctt gtcaaggacg gcgccgacat gctggatatc
     1021 ggcggggagt ccacccgtcc cggcgcgccg ccggtgccgc tggacgagga actcgcgcgt
     1081 gtgctgccgg tcgtgcgcca tgcggtgacc ctgggcgtgc cggtgtcggt ggacacctac
     1141 aagcccgaag tcatggccgc ggtgctggac ctgggggccg acatcgtcaa cgacatctgg
     1201 gcgctgcgcc agcctggcgc agccgccgtc gtggccgcgc atcccgcgtg cggcgtctgc
     1261 ctgatgcaca tgcaccgcga gccgcagacc atgcaggccg cgcccatgga gggcgacgtg
     1321 gtgccgcagg tgctggcttt tctggcccag gccgcaacag agctgcgcgc gctgggcgtg
     1381 gctgccggcc gcatcactct ggatccgggc atcggtttcg gcaagaccgt ggcccagaat
     1441 ttcgccttgc tggcgcgcca gcacgagctg ctggcggccg gttacccgct gctggccggc
     1501 tggtcgcgca agtcctcact ggccgccgtg agcagcagtt ccctggccca ggtggcccag
     1561 ctcgacatca tcgaccgcat ggtgcccagc gtcacggccg ccgtgctggc cgtggaccgc
     1621 ggtgcgcggg tggtgcgtgt gcacgatgtg tgcgaaaccg tgcaggccct gaaggtctgg
     1681 gtggctgcca cggcgccgca gcctacaagc gggcaggtcg gcgcaccgta gaatttccgc
     1741 tggtattgaa atcgaatcca taaccaccag ggacaaacaa caatgacaag aaaatacttc
     1801 ggcaccgacg gcattcgcgg caccgtgggc caggcgccca tcacgcccga ttttgtgctg
     1861 cgcctggcgc atgcggtcgg ccgcgtgctg aaaaagaccg aggcccgacc caccgtgctg
     1921 attggcaagg acacccgcat ttccggctac atgctcgaaa gtgcgctgga gtcgggtttc
     1981 aactccgccg gcgtcgatgt ggtgctgctc gggcccttgc cgacaccggg cgtggcctac
     2041 ctcacgcgca cgcagcgcgc cagcctgggc gtggtgatca gtgccagcca caaccccttt
     2101 cccgacaacg gcatcaagtt tttcagcgcc cagggcgcca agctgccgga cgcgtgggaa
     2161 gaggaagtcg aagccgtgct ggaagaagcg ccggtgtggg cggactcgcc caatctcggc
     2221 aagacgcgcc ggctcgacga cgccgccggc cgctacatcg agttctgcaa aagcaccttt
     2281 gccaatgacc tgacgctcaa gggcatgaag atcgtcgtcg atgcggcgca cggtgcggcc
     2341 taccacattg caccggaagt ctttcatgag ctgggggccg aggtcattgc catcggctgc
     2401 gcgcccgacg gcatgaacat caaccacgag gtcggcgcga cccatcccga ggcgctgatc
     2461 aaggctgtca aggcctatgg ggcggattac ggtgtggcgc tggacggcga cgccgaccgg
     2521 ctgcaactgg tggacgccca gggtcgcctt ttcaatggcg acgaagtgct gtacctgatg
     2581 gtggccgaac gcctggcgcg cggcgagaac gtgcctggcg cggtcggtac cctgatgacc
     2641 aacatggccg tcgaagtggc gctgaaggcc cggggcgtcg agtttgtgcg cgccaaggtc
     2701 ggtgaccgct atgtgctgga agagcttgaa aaacacggct ggctgctcgg cggcgaaggc
     2761 tccggccacc tgctggcact ggacaaacac agcaccggtg acggcctgat cagcgcgctg
     2821 caggttttgc aggcctgtgt gcgcagcggc aagtcgctgg cccagctgtt gtccgatgtg
     2881 acgctgtttc cccagaccct gatcaacgtg cgcttgcagc ccggccagga ctggaaaagc
     2941 agcgcggcgc tggcagacat gagccgcacc gtcgaagccg aactcggcga cagcggccgg
     3001 ctgctgatcc gcgccagcgg caccgagccg ctgctgcgtg tcatggtaga ggcgcgcgac
     3061 gcagcgcagg ccctggcctg tgccgagcgc gtggcggaca cgatcaggcg ctgacgccgt
     3121 tttttctttg aatgaatagt gcctctggcc cagagtggac gggcgcgagc agctatcaaa
     3181 aaaagagcgt ctgtgcgggg tggcattggc agcgcgccca tgctctctgg cggatgaatt
     3241 catgacgtgg atcaagtttt ttcaggcagg tttgcgtaag cttgcacatt acctgaggag
     3301 acttgaacat gccgaatctt gaatggtctg acgcgctcgc gctcgacctg cccgcgatgg
     3361 atgacaccca ccgggaattt gtcgacctgc tggcgcaggt ggaaaacgcc gcagaccacg
     3421 cgctgctggc ccactggcgc gtcttgctgg accacaccga tgaacatttc agccgggaag
     3481 accgctggat gcaggacacg cggttttcat cgtccaactg ccacagcgtg cagcacaagg
     3541 tggtcctgca ggtcatgcgt gaaggcctga ccggcggcga agccggcaac ctgaaaatgc
     3601 tgcgcgacat ggcgcgtgaa ctcgcgctct ggtttcccca gcatgcccag accatggatg
     3661 ccgcgctggc cctgcatttg cgcggcatcg gttacgaccc ggtcaccggc gtggtggcca
     3721 tgcctgcggc cttgcccagg gacatcattc acggttgcgg cggcgccagc tgcaccgata
     3781 cccaggcgcc cgcagccacc gcgcaggcgg catgagtacg gcgccagccg ttgtggccgg
     3841 cgccatgggt gtgccacacc accgcctgca cgaggatgtg caggccctga ccacgggcac
     3901 cctgtttgtg gcgctgggcg tggtcatgtt cggtcatgcc ggactgttga cgggtggcac
     3961 ggccggcatc gctttcctga ttcactacgc caccggcctg agtttcagcc tcgcgttttt
     4021 tatggtgaac ctgccctttt atttctttgc ctggcggcgc atggggctgg ccttcaccct
     4081 caagaccttt gcggccgtgg gcctgctgtc gctgttcact tgggtcctgc cgcaggggct
     4141 ggccttcggc tacctgtcac ccgcgctggc ggcggtgctc ggtggcctgc tctgcggcgc
     4201 aggcatgctc atcgtgtttc gccatggcgc cagtctgggc gggctgaaca tcgtggtgct
     4261 ttatctgcag gatcgcctgg gctggcgcgc cggcaaggtg cagatggcca tggacggcct
     4321 gatcgtgctg ggtgcgctgt gggtgaccga ctggcagcgg gtcgcgctgt cggtgttggg
     4381 cgcggtggtg ctgaacctgg ccctggccat caaccaccgg cccggccgct acatggcgat
     4441 gtagcggctt ttcttcaggg ccggacctcg gccccaaggt ctatttaccg gcgcgtttgg
     4501 cgtcctgatc catcttgccc atgaacagca tgaagaaaaa cacgcccatg ccaatcatga
     4561 aggcaatgcc gatgacgctc atgaggccat agtcggtgga gaacaggtca gtcaacagtt
     4621 tcatggtggt cgctccttgg taaggcttcc actatggcct gtctgcctga aacacatact
     4681 gatgcaggtc aacaatgtat gcctgaagcc gccctccggg cctgggaacc gggttgattt
     4741 gtacaagatt cagggctgca gcccaatgaa tacgtgcgct gggcgctact gaaaacgaag
     4801 catggccgga tcgctcaggg cgacggctcc gcctgccagc cgccgcccag cgactggatc
     4861 agcgccaccg ctgtggtctg gcggtccgcc atggcctgca ccagcgcgcg gcgcgcgctc
     4921 aatgcggtgg cctgcgctgc aatgacttcg gtgtagccga cctggccgct gcggtagcgg
     4981 ttgagcacct gctgctcgac ctggtcggcc gatgcggagg cctggcggcg cagctcctgc
     5041 tgggtgagca gcacgcgggt ggcggccagc tggtcctcga catcctggaa ggctgccagc
     5101 acggtttgcc ggtaacgtgc cacggtttgt gcatgggccg cttcgctgcc ttccacgcgg
     5161 gcaccggtgg caccggcatt gaacagcacc tgtgcggccg acaggcccag cgaccagacc
     5221 gtgctggacg cgctgaacag gtccgccacc cggctggcgc cgaagccata ggaggcactg
     5281 agggacagat tggggtaata cgcgcttttg gcgatgccga tctgctcgtt ggccgccgcc
     5341 acgcgacgtt cggccgcggc gatgtcgggc cggcgctgca gcagcgttga cggcacaccc
     5401 accggaacct cgggcacgga aggtttccac gccgcccggg cgagcgagaa attgcccggc
     5461 gcctcgccga tcagcaccgc tatcgcatgc tccagctgcg cacgctgccg caccaggcca
     5521 gcatcgtcag cctgggcgtt ggccagctgg gtttgcgcct gcagcacatc ggtcttaccg
     5581 acaatgccgg cgttgtagcg gttctgtgca atttccagtg cgcgttggta gccggtgagg
     5641 gtgtcctcga gcaggccttt ttgcgcgtcc agctggcgca gtgaaaagta gttgatggcc
     5701 agctcgcctt gcgccgacag tttggccgag gccaggtcgg cggcgctggc ctgggcgccc
     5761 gcggtggcac catcgaccgc gcggccgagc cggccccaga cgtcgggctc ccagctgccg
     5821 cctatgctga gctggtagtt gttgccggtg cgcccgcccg agctgcctgt cgtgcttgtg
     5881 ctgctactgc tgccaccgct gccaccgctg ccggagcggg tggcaccgcc gcccagcgtg
     5941 accgcaggaa agagggaagc gcgctgctcg cgcaccagcg cacgcgcctg ggcgtaggcg
     6001 gccacggcgg cggccacatt ctggttggat acctccaccc gcgcggccag ccggtcgagc
     6061 accgggtcgc cgaacagcga ccaccagggg ccgcgttcca gcgcatcggc cggtgcagcg
     6121 ggcacccagc cttcggcttc cttgtagctg ctgacctctg gcgtgagcgg ccgctggtag
     6181 gtggggccaa cggcgcagcc gccgagcaga ataacaagcg cggcggcgat ggggtaaagc
     6241 gtgagtctgg atcgcatggc gggcctcatt gcagttgcag cgccgcaccg ggtagtggcg
     6301 cgctggcatc atcgggatga cggctcaggt ggcgctcgga cgggctgcgg cggcgcagct
     6361 tgtcgagcag gatgtaaacc accggcgtgg tcagcagcgt gagcagctgg ctggcaatca
     6421 ggccgccgat gatggagatg cccagcggcc ggcgcaactc ggcaccttcg ccaaaaccaa
     6481 tggccagcgg caacgcgccc aggatggcgg ccagcgtggt catcaggatg ggccggaagc
     6541 gcagcaggca ggcctcgcgc accgcctcga cggccgacag gccgcgtgaa cgctcggcct
     6601 cgatcgcgaa gtcgatgatc aggatggcgt ttttcttgac gatgccgatc aacaggaaca
     6661 cgccgatcag cgcgatgatg gaaaactcca tcttgaacag catcagcgcc agcaccgccc
     6721 ccacgccggc cgagggcagg gtggacagca ccgtgatcgg atgcaccagg ctttcgtaga
     6781 ggatgcccag cacgatgtag atcacgatca gcgccgccag gatcagcagg ggctgctcgt
     6841 tttgcgattg ctgggcgctg agcgccgttc ctgaaaaact gccgcgcaca ttgctgggca
     6901 ggcggatctc ggcctcggcc tgtttgacgg cgtcctgccc ggcgctgagc gggatgccct
     6961 cggccaggtt gaaagacacc gtggtcgaaa gctcgccgtc ttcatggctg atcgatgcag
     7021 ccgtcgggtt ttcgctgaag ctggcgattg cggacagcgg caccatggtg gtgaccgctg
     7081 cgctcagcgc ctggccagag gaggggtcgc gcagcgccgg gttggcgacg ctggtgctgg
     7141 tggacggggt tgcagcggcc agggtcgtgc cgctaccgcc gcgtgcggga acataaatgt
     7201 ctttcagcga ttccgggctc tggatgtaac gcggcgccac gcccatgatg accttgtact
     7261 ggttcaggtc ggcataaatc gtggcgacct ggcgctggcc gaagccgttg tacagcgcgt
     7321 tgtccacatc gcgcgaactg atgcccagcc gcgccgcgct gtccttgtcc acggtgacca
     7381 tggtctccac gccgttttcc tgctggtcgg tgtccacgtc ggtcagcgcc ggttgcagtt
     7441 tcatctggtc ggccaggcgc acggcccaga ttttcaggtc gttgttgttg tcactcttca
     7501 gggtgtactg gtaggtggag ttgctggccc gcccgccccc gcgcaggtcc tgcaccgggt
     7561 tgaggaagat gctgatgccg gtgatctgcg ccagttgcgg acgcagccgg gtgatcaccg
     7621 cctggccctt gtccgtgcgc tgattcaccg gcttgaggtt gatgaacatg aagccgccgc
     7681 cggcgcgcga gccgccggta aaaccgacca cggtatcgac cgccgggtcg gcccggatga
     7741 tgtccacgat ggccttgagc ttgctctgca tggcctggaa ggaaatgctg cggtccgcgc
     7801 gtatgccgcc gctgatctgc ccgctgtcct gctgaggaaa gtagcccttg gggatggcga
     7861 cgaacagata gacattgagc gcaatgacgg ccagcaggct cagcatcacg atcactcggc
     7921 tggccagcgc ccagtcgagg ctgatttcgt agccgcggtg caggcgcctg aacgccgcct
     7981 cgaaccagcg cgccacccgc cctggcggct tggtgtgggc gtcgggcttg agcagccagg
     8041 cgcacatcat gggggtggtg gtcagcgaga tcagcagcga gatcagcacc gccgccgaca
     8101 gcgtgaccgc aaactcccgg aacagccggc cggtttgccc gcccatgaac agcaggggaa
     8161 tgaacaccgc caccagtgac aggctgatgg acagcacggt gaagcccacc tcgcgcgcgc
     8221 cgagaagggc tgccttgaag cggtccatgc cggcctcgat gtgccggctg gtgttttcca
     8281 gcaccacgat ggcgtcgtcc accacaaaac cggtggccac ggtgagcgcc atcaggctca
     8341 ggttgttcag gctgaagccc aggaaataca tcacgccgaa ggtgcccagc agcgacacca
     8401 cggtggccac ggccggaatc agggtggcgc gcgcgctgcg taaaaacgcg ctgaccacca
     8461 gcaccaccag cgcgatcgag atcagcagcg tgatctcgat ctccttgagc gaggcacgga
     8521 tcgagttggt gcggtccgag gcgatgtgca gcaccacgtc ctggggcaac tgggcctgca
     8581 gctcgggcag cagggcgcgc acgccgtcca ccgtggcgat cacgttggca tcgggctggc
     8641 gcgtgaccag cacgatcacg gccggctcgc cattgaacag gcccagggtg ttgatgtttt
     8701 ccacgccatc gaccacctcg gccacgtcgc gcaggcggat cgccgcattg ttgcgccagg
     8761 ccaccaccag atcgcggtag tcggccgcct tgcgcccgcc gctggccgtg ctggcacccg
     8821 aatagatctg caggcgctgg ccgttgcctt caatcgcgcc cttgggccgg ttggcgttgg
     8881 acgcttgcag ggcggcgcgc acatcttcca tgctgacgcc gtaacggttc agtgcaaacg
     8941 gcagcaggtc cacccgcacc gcaggcagcg agccgccgcc gagctcgacg tcgccgacgc
     9001 ccggcacctg cgcaattttc tgctgcacga ggttggagac ttcgtcgtag atctggccgg
     9061 gcgcgcgggt tttggacgtc agcgccagga tgaggaccgg agccgacgcc gggttcgcct
     9121 tgcggtaggt cgggttgctg cgcaaggtgg cgggcaggtc ggcacgtgat gcgttgatgg
     9181 cggcttgcac ttcgcgcgcc gctgcgtcga tcttgcggtt caggtcgaac tgcaggctga
     9241 tgcgggtcga gcccgtgccg ctgctggacg tcatttcatt gacccccgca atcgtgccca
     9301 ggcgccgctc cagcggcgtc gccacactgc tggccatggt ggacgggctg gcgccgggca
     9361 gcgaggcact gaccgagatc gtcgggaaat cgacctgcgg cagtggcgag accggcagca
     9421 cgaaaaacgc gcctatgccg gcgagggcca ggccgatggt cagcagcact gtggctaccg
     9481 ggcggcggac gaagggttcg gagaggttca cttgccggtt cctcgtgtcg ccggcagggc
     9541 ggttgcgcat tgagccagtt tcagtggcga tgcacaagct ggttggccgg gaggtgtccc
     9601 ttcaaccgtt cgggctgagc ctgtcga
//
LOCUS       DAIDFG010000095         9595 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_95_length_9595_cov_16.762788, whole genome shotgun sequence.
ACCESSION   DAIDFG010000095 DAIDFG010000000
VERSION     DAIDFG010000095.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9595)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95_length_9595_cov_16.762788"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1810 a         3072 c         3053 g         1660 t
ORIGIN      
        1 ccagatgcgc cgtcagcgcg acttgcgacg tgcccatcgc ccagatgtgc aggtcgtgca
       61 cccgtgtcac gcctggcagc gccgccagac atgcctgaac ggcctgcggg tccacgctgt
      121 cggggacgcc gtcaaacagc atgtgaagcg actgcttgaa aaggctcgcg gttcccgcca
      181 ggatgacgcc cgcaatcagc aggctgacca ccgggtccag ccacacccag cccatccaca
      241 atgtcagcgc ccccgcgacc accaccccgg cagacaccag ggcgtcggcg gccatgtgca
      301 tgaaggcgcc gcggatattg aggtcgtgct cgcgcccgcg catgaacagc agggcggtgg
      361 cggtgttgat cacaatgccg atgccggcca ccaccatgat ggtcacgccc tcggcaccgg
      421 ccagcggctc gggcgagaaa aggcggccca ccgcttccca ggccagtgca cccatcgcca
      481 tcaggagcag cagggcattg gcaaaagccg ccagaatggt gccgcgcttc cagccgtagg
      541 tgtggcgcgc gttggggcgc agcttgctgg ccagtgcgcc gccccacgcc aggaccagac
      601 ccgccacgtc gctcaggttg tgcccggcgt cggccagcag ggccagcgag ttgattttcc
      661 agccgtaaaa cgcctcgatc gccacaaaga cgatgttgag cacaatgccg atggcaaaag
      721 cccggttgaa gttggcgggc gcgtgcccat ggtcgtggtc gtggtcagcg ctcatgcggc
      781 gggggcctcc ggcatcaccg cgacactgcc cggcgggaaa taggcgggtg ggtccgacgc
      841 atcgaaggat tcctcgaatg cacggcccca ggcggccatc atggactcgc gttgactgcc
      901 ggcttcctca cacatccacg ccctgatttc ttttgcgtat cccatgcaca tgtcgcttgt
      961 cctctcgttg cgatgtcgat gttgcttgcg gttcagcgcc gccgcaggac accactcaag
     1021 tcagttcccg gcgcaacggt attgaacacc gtgccgtatt tcttcacatt ttcatgcagc
     1081 aagtccagtc gctggtccga ctcgtcggta tccacgatga tttcgtaacg gatggactcc
     1141 attcggggcg gcacgtcctg ccgcacgcca tcgacgatga cttcaatacc gcgcatcctg
     1201 aacttcagaa taggcgccac ccgctccatt cccttgatca tgcaggccga gagggccgcc
     1261 agcaacagct ctgccgggtt gaatgcatcc gggttgcccg ccaggtcggt atccagggcg
     1321 atttcggcgt ttttgcaatt cgacaggcta ccgtgcgcgt cgacgcggcg cgacgccaca
     1381 tgaaaagtca ttttggcggc gggctggctc atgaagggtg atccttgtcg gttgcgttcg
     1441 tactcagggc aggcgcggca caagcgtcaa ggtgcccccg acgacagggt aacccttttg
     1501 cagcggggga acgcgctttc gttgctcaag cggtggcagt aagccactgc tcgatcttgt
     1561 cacggctggg cacgccgccc gcatggacca ccttgccatt gatgacaaca ccgggtgtgc
     1621 tcatcacgcc gtagcccatg atgtcgcgca attcctccac tttttccaga tggatggtga
     1681 cgcctttggc ttttgccacc tggtcgatga gggcgatggt gttcttgcag ttggcgcagc
     1741 cggtgccgag taccttgatg tccatggtgt ttccttgaga ttgaagttga tgaaataaag
     1801 ctgtgcctac agcaccgcgt tgaatacaaa acctaccagc aggatgccgc cggacaccac
     1861 cgccacaaag gtgacaatca gccgcagctt gagcaccttg cgcaggatga tcagctcggg
     1921 cagggacagc gcaatcacgc tcatcatgaa agccagcacg gtgcccaggg ccgcgccctt
     1981 ggccagcagc gcctgcacga tgggaatcac gcctgctgca ttggtgtaca tcggcacacc
     2041 catcagcacc gccagcggca ccgaccacca ggcttccttg cccatgaagc tggccatgaa
     2101 cgcttccggc acatagccat ggatcaaagc gcccagtgtt atgccgccca ggatgtaggg
     2161 ccagaccttg cccacgatct cgcgtaccgc tgaaaagccg gcttgaatgc ggtcagccag
     2221 cgtggtctgc gcgtcttcaa actgggccga agttttgggc atgtcgcgca cccagtcttc
     2281 cagccaggct tccatcttga ggcggccaat gacccagccc gcgacgatgg cgaccgtcag
     2341 gcccaggccc aggtacaaca gcgccacttt ccagccgaac aggccaaaca gcaacgtcag
     2401 cgccacttcg ttgaccatgg gggcagagat caggaaggag aacgtgaccc ccagcggcac
     2461 ccccgcctgc acaaaaccga tgaacagcgg caccgcggag catgaacaaa acggcgtgac
     2521 gatgcccagg gacgcggcca tcacgttggc cacgccctcg ctgcgaccgg ccagcagggc
     2581 gcgggtgcgc tccggcgtga agtagctgtt gatcatgccc atgacaaaca ccacgccggt
     2641 cagcaacatc agcaccttgg gcgtgtcgta gaagaaaaac tgcagcgcac cacccaggtg
     2701 gctggcccgg tctaccggca aggcggcgac cagtgcttca gagccgggaa gcagtgtctg
     2761 gtaaaggccc agccagacca gagcggccag gatcaaaaag atgaccggct ggcgcagggg
     2821 ccaggatttt tgcggcgtcg tgacggtgtt catacccatg aagacgaacg gaatcggcaa
     2881 aagacgcaca aatcgcagaa ataatgatga aatatgcctc cagcccttat gcagaaagcg
     2941 caagaagcta tcaaaatagt agcaaaagag ggtgtaggca cggctgcggg gccggtgctc
     3001 agcgttcttt caagccgcca gctctacgga cgcggcacaa aatccgcgac gtgcccggta
     3061 tcgtcgattt gcacctggca agccacggtc atgcgctgct tcaggcgctg gcgtgcgcgc
     3121 tgcaggcgcg atttggcggc gctgaggttc agccccctgg cggcggcgaa ctcggcttgg
     3181 ggcatgcctt gcaggtcaca gagcgtgatg acctcgcggt cctcggccgt cagctcagag
     3241 agcacgcgcg gcaggcaggc cgtgagggtg tccaccgtgt cggcttcgtc ggcaggtgcg
     3301 gccaggtcgt cgggtacttc cacggtcgcg cgggccacgc gcagccgatc ggccagtgtg
     3361 ttgcgcgcca cctcgaacag ccaggcgcgg gcgttttgca ccgagcagaa gcgttcaccc
     3421 tggcgcaggg ccttgatgaa caggtcctgc agcaggtcgt cggcctcggc agggttgccc
     3481 agccgatggc gcagccagcc gcgcaactcg ggcgcgtggg tggcccaggc ggtggtcaga
     3541 caattcggtg cggctccgtt caacatggtt tcctcaggca ctgatggcca gccgcttcat
     3601 tttcgcccaa ggtgccatgg ggttggtgac gtcattgctt ttcctgctgc agggcaggag
     3661 cgatgccgga acggacagtt cactgaaatc gcctgaattt caagtcagtc agcccccgcc
     3721 cacgaaggac gggcgcaggc agctacgcaa ttcatagtct gcgcagtcac caactgactg
     3781 tcatcaaact gccaccaaac cgacatgaac acgtcaccct cgacttccag aatcaccgca
     3841 tcagtcaaaa acacgatgga gagtgattca tgggtcaagg tttcggcagg gattatgagc
     3901 agggtgaagg cgtcaacacg acgtgcaatc cgttcattca tgaagtagac ggtgagcgtc
     3961 gccatgtgct gcgttgcacg acggcggcgg tggcgctgct gatggggtcg cacacgctga
     4021 cggccagcgc cgcggcgccc gccaaaggca ggccgctcgg ttttaccggc gtatcgactt
     4081 ccagcgccga cgcgctggtg gtgccgcccg agtacgaagc ccaggtgctg taccgctggg
     4141 gcgacgcgac cggtattgcc ggggctgcgg caccggcctt cagtgcggat gcgtcgaaca
     4201 gctggcagga gcagctgctg cagtcgggca tgcaccacga cggcatggag tttttcccgc
     4261 tggacaagaa cccgcgccac ggcctgctgg ccatcaacca cgaatacacc gacgacggcc
     4321 tgctgcacag cgacggcatg aagacctgga ccgccgacaa ggtgcgcaag tcgcaggcgg
     4381 cgcacggcgt cagcgtgatc gaggtggtgg aacgcgccgg tgtctggcgc gtgatgccca
     4441 actcgcgtta tgcgcgccgc atcaccggca acacacccat gcagttcagc ggcccggccg
     4501 ccgggcatgc gctgctgaaa acttcggccg atcccgaggg catgcgcccg ctgggcacgc
     4561 tgaacaactg cgccgccggc cagacgccct ggggcacctt cctgacctgc gaggaaaact
     4621 ggaacggtta cttctcggcg caggacaagc cgaacccgga cgaggcgcgt tacggcctgc
     4681 gtgccaaggg ctttggttac cgctggcacg agtttgacga acgttttgat gtggccaaac
     4741 acccgaacga gccacaccgc ttcggctggg tggtggagat cgatccgcag gacccgacac
     4801 agactccgat caagcgcacg gcgctgggcc gcatcaaaca tgaaggggca acggcgacgc
     4861 tgtcccgcga tggccgcgcg gtggtctaca tgggtgatga cgagcgtttt gagtacatct
     4921 acaagtttgt cagtcgcgac aaggtcaagc ccggtggtta ccgcgccaac aaggacattc
     4981 tggaccacgg tacgctgtat gtggcgcgct ttgatgcaca gggttacggc cgctggctgc
     5041 cgctggtgca tggcgaagac ggcctcaatg ccgccagcgg ctttgccagc caggccgacg
     5101 tggtggtgaa gtgccgtcag gcggcagacg tggtgggtgc caccaaaatg gaccgccccg
     5161 agtgggtggc ggtgcacccc aacaccggcg aggtgtttgt cacgctgacc aacaacagcc
     5221 gccgcggcca gggcggccgc atggccgacg ctgccaatcc gcgcaacgac aacatcatgg
     5281 gccacatcat ccgctggcgc gaaggcaaga gccgtcaggg cgacgctgtc agccggctcg
     5341 acggcgacgc cagcgccacc gagttccgct ggatgcactt tgccctggca ggtgacccga
     5401 atgccgacaa ggccgaatac cgcggcacca tcaagggcga catgtacggc agccccgacg
     5461 gcctgcagtt cgatgcgggt ggcctgctct ggatacaaac cgacatttcg ggatcggcca
     5521 tgcacaaggg tgcctacaaa ggcataggca acaacatgat gctgtgcgcc gacccggcga
     5581 ccggcgagac caaacgcttt ttgaccggcc cggccggttg cgaggtcacc gggatgagct
     5641 tcacgccgga ccgccgcgcg gtcttcatca acatccagca ccccggtgaa accgccggcg
     5701 cgcgcggcaa tccggcccag ccgaaggcgg tgagccattg gccggacggc ccggcgggcg
     5761 gacggccgcg ctcggccacc gtcgtcatcc ggcgcaagga tggcggcatc gtcgggacct
     5821 agtttccaag cagcaacagc cccgacctcc ggggcttttt tacagaattt ttgagtgccg
     5881 gtatcgagag ttgagtgtcg agagtcattt gcgagaagac aaccaggagt ccatcatgaa
     5941 agaactgccc aatcccacct ttgcattgtc gccagtcgca atgccacggg ggtcacttca
     6001 tcaaagctct catgctgcca atccggtcgc tgttcaaggg gctgcgccgg ggaaaatcca
     6061 ggtaggctgg gcccggcacc aggacgaggt ccggcaggcg cagcggctga ggtaccaggt
     6121 cttcgcgctg gagatggggg cgacgctgcc cagcacggtg ccgggccacg acatcgacct
     6181 gttcgacgat ttctgcgaac acctgctggt gcgcgacgaa gccagccagg aagtcatagg
     6241 cacctaccgc gtgctgacgc cgacccaggc caaacgcgtg ggcagcacct acagtgatac
     6301 ggagttcgac ctcacgcgcc tgcgcgccct gcgcgagcgc atggttgagc tggggcgcag
     6361 ctgtgtgcat gccgactacc gccacggcgg tgtgatcctg gccttgtggg gcgcgctggc
     6421 cgagttcatg gcgcgcaacc agctcgacac catgattggc tgcgccagca ttccgatgca
     6481 gcacagcggc catccggaat acggcagcgg gcatgccgcg gccagcatct ggcgccaggt
     6541 gcggcaaagc cacctcgccg ccatcgaata ccacgtgacg ccgcgcctgg ccctgccggt
     6601 tgagcagctc gacgactcgc tggacgtcga gccgcccgcg ctgatcaagg gttacctgcg
     6661 cctgggcgcc agggtgctgg gcgcgccggc ctgggacccg gacttcaatt cagccgacct
     6721 gcccatgatg atgcgtattg ccgacctgcc gccgcgttat cgcaagcatt tttccgcgag
     6781 ggaggcctga tgcgtacgct gttcgacaag cttggttcgc tcaaccccgg tctcccgcaa
     6841 ggtggggacg atgcgcatga cgacgccgca ctgcgcaagc gttaccgcag catcttcatc
     6901 tccgacctgc atctgggcac gccaggctgc caggccgcgg cgctgctgga tttcctcaag
     6961 gcgcacccga gcgactacct ctacctggtg ggcgacatcg tggacggctg gcaactgcgg
     7021 cgcaaatggt actggccgca gtcgcacaac gacgtggtgc aaaagctgct gcggcgcgca
     7081 cgcaagggct gccggattgt ctacgtgccc ggcaaccatg atgagtttgc gcgtgaattt
     7141 atcggccacc agttcggcgg catcgaggtc atggaagaag cggtccacgt cacggccgat
     7201 ggccgccacc tgtggatcgt ccacggcgac tatttcgatg ctgtcgtgca gtgcgcccgc
     7261 tggctggcct atgtgggcga caacctgtat gaattcacgc tcaagctcaa ccgccacctc
     7321 aacagcctgc gcgcgcgcat gggcctgccg tactggtcgc tctcggccta cctcaaacac
     7381 aaggtcaaga gcgcgctgaa ctacgtcacc gactttgaag tcgccgtggc caacgaggcg
     7441 cgccggcgcg gccaccaggg ggtggtctgc ggccacattc accgcgccga aatgcgcgac
     7501 atcaacggcg tgctgtattg caacgacggc gactgggtcg aaagccgcac cgcgctggtg
     7561 gaacactacg acggcaagct ggagctgctg cactgggcgc cgtcccatga aacccatcga
     7621 agggagccac tgaaaagtgg aattagcgga gaggtctccc ctctgttcga gccggggccg
     7681 cggaactggc ttggccaggc cgcaggcgca gcggcccccc cggggggcag ggcgctacac
     7741 gaagtgagca accgtggggg ccagttacag tcatgaagat agcccttgtt accgacgcct
     7801 ggcagccgca ggtcaatggc gtggtgacca cgctggtgga actggtgcgt gaactgcagg
     7861 ccctgggcca cacggtggaa gtcatccatc ccggcctgtt caaaacgcgg ccttgcccgg
     7921 gttatgccgg catcgacctg gccgtgcgcc caaaagcctt gctgaccggg cggctggatg
     7981 cctttgcgcc cgaagccatc cacatcgcca ccgaaggccc gctgggctgg gcagcgcgca
     8041 gctattgccg caagcggggc ctgcgtttta ccactgcgtt ccacaccaaa tttccggaaa
     8101 tcctccatgc cgcgttgaaa ataccgctgt cctggggtta tgcgctgttc cggcacttcc
     8161 acaaggcctc ctccggggtc atggttccca cccacggtgt gttgcgcatg ctggagcagc
     8221 gcggttttcg ccagctgcgc agctggacgc atggggtgga caccgccttg ttcccgtttc
     8281 atcccgaggc gcggctctac ccccgcatgg gcacgctggt gcggccggtg tcgctgtttg
     8341 tcggccgcgt gtcgtatgag aaaaacatcg aggccttcct ggcactggac attccgggga
     8401 ccaaggtggt ctgcggtgtc gggccgctgg aggcgtcgct caaggagcgt tatccgcagg
     8461 tacgctggct tggcgtgctg ccgcgcgacg agctggccaa ggtgtacgcc gccgccgatg
     8521 tgtttgtttt ccccagccgg tcggagactt tcggcctggt gatgctcgaa gccatggcca
     8581 gcggcacgcc ggttgcggcc tacccggtcg acggtccgct ggaggtgctg ggcaagccgg
     8641 acggcagccg ccacggcggt gtgctggatg aggacctgaa gcacgcctgt tacagcgcga
     8701 tggcggtgcc gcgccatgaa gcgcgcgccc gttcgctgga cttcagctgg gcacatgccg
     8761 cccagctgtt cgaagggtat ctggtgccgg tacgtggcca ggccaccagt cccctggtga
     8821 cggcgcaagc agccgcctga aacatttgac atgatgttgt cacgcatttg tcatcaaacc
     8881 agcgcacact gacaagtcag attcatgaca attaccagtt cggaccgcat gtttcaaact
     8941 tctgccaatt cgctcccggc tgtgagcgcc cctgccgcgg ctgcagccgt ccagtttctg
     9001 gaccgggatc acagcatcct ggctttcaat gagcgggtgc tggactgggc caggcggccc
     9061 gacgtgccgc tgctggagcg gctgcgcttc ctgtgcattg tttcgtccaa cctggacgag
     9121 tttttcgagg tgcgcgccgc actgcacctg acggctgcgc tcaatcagga gcagaaggcg
     9181 ccttactcgg ccgagtcttt caagaccctg tctgccgccg cccacgacat ggtggcgcgt
     9241 cagtacgcgc tgtacaacga cgagttgatg cctgaatttg cgaagcaggg catccggatc
     9301 gtttcccacg gcgaccgcag tgcggcgcag cgccgctggg tcaaggccta cttcgagcgt
     9361 gaggtccggc cgctgttgat tccggtcggg ctggaccctt cgcatccgtt tccgcaggtg
     9421 gccaacaagt cgctcaattt cattgtccgg ctcggcgggc gcgatgcctt cggccgcgag
     9481 aacgacatcg cgatcgtcaa ggtgccgcgg gtactgccgc gcctgatcca catgcccgac
     9541 aagggctcgc agaaaaagct gctgtttgtc tcgttgtcga gcatcatccg ggcgc
//
LOCUS       DAIDFG010000096         9532 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_96_length_9532_cov_18.010763, whole genome shotgun sequence.
ACCESSION   DAIDFG010000096 DAIDFG010000000
VERSION     DAIDFG010000096.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 9532)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9532)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9532
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96_length_9532_cov_18.010763"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1731 a         3185 c         2915 g         1701 t
ORIGIN      
        1 tcctgagcct gtcgaaggac gccccccgag ggccgctgcg cctgcgggcc ggcggagccg
       61 gacccgcggc ccctgcttga aaagagatgc ctgcgcggtc gcttcttttt atccgttcgc
      121 cctgagcttg tcgaaggctg ccagtcgtga agccgcaagc tcagggcttg gtattaccag
      181 ccgttttgag gcccgccacc gtcgcacgcg gatgcgcggc gtcgtaaacc ttggccaggt
      241 gctggaagtc gagccgggtg tagacctgcg tggtggtgat gttggcgtgg cccagcagtt
      301 cctgcacggc acgcaggtcg ccgctggact gcagcacatg gctggcaaac gaatgccgca
      361 gcatgtgcgg atgcaccggc gtggccagac cggcctgcag ggagcgctgc ttgagccgca
      421 cccggatgtg ttggggcgtc aggcgggtgc cgcgctggtt gatgaacagc gccgcgtctt
      481 ctttggccca ggcgctgcgc agtaccagcc agtcctgcag cgcctccagg gccttgctgc
      541 ccaccggcac gctgcgccgc ttggacccct tgccgagtac atgcgcctcg gcagcctgca
      601 tgtcgatcca gccgcgcgcc tggctgctgg ccacgacatc cagcccggtc aactcgccga
      661 tacgcaggcc gcagccgtac agcagttcgg tgatgcagcg gtcccgggcc tgcagggccg
      721 cgtcgccagc ggtgccgtcg ctgcgccagg cggccagctg caccgcttca tcaacactca
      781 gcgccttggg cagcggtttg gcggccttgg gcgcgcgcag gtcctgcacc gggttgctgc
      841 tgaccaggcc ttcgcggccc agccaggtgt aaaagccgcg ccagcccgag aggatgagcg
      901 caatgccacg cccgctgcgc ccgccgctgt gcatctgggc cacccagcgc cgcatgtgga
      961 tgttttgcgc ctgggtcagc gccacgccgg cctgccggca gttgacctgc agtttttgca
     1021 ggtccagcgc gtacagctcg acggtgcgct gcgccagccg cttttcaacc cgtacgtgtt
     1081 cgaggtaacg ctcgaccagc gggtcgttgt cgggaaccgg catcgcggtc ccgtcaacgc
     1141 tggcgtccgg cacggccatg ccttatgggc tagtcggcca tggggcgcag gcgcgacagc
     1201 gccgcgccgg ccagttcgcc gatgcgttcg aggaagtcgg tgcccatgct ggcgctgaaa
     1261 cgctcggggt cgggtgaagc cagcaccagc atgccgaagg cctgcggcga cttgccggga
     1321 cgcagcggaa tcagcgcgac cgacagggcc tgcgccggat cgggcagcca gctgaccgcc
     1381 tcgaaaccgg aattgacacc gcagtaaggt gcggtcagcg aggacgcaaa ggtctgcgtg
     1441 tcttcgctga cgcctgttga aaagtcttcc atcgaaaaaa tgccgttgac gtgccagacc
     1501 ttgatggccg cctgcggcac ggcgaactgc gtctggatct cgcgcacgat ggtgtcgggc
     1561 aggtcgcgcg catgggcggt cagcagcagg ttcagcgtgc actgctgcag cttgtcggca
     1621 atgcccacgt tttcctggcc gtggcggatc atgtcgacaa cccgggactc cagcaccttg
     1681 atcttgtcgc gcaacatgcc ggcctggcgc tcctgcaggc tcaccgcccg gctgccgtgg
     1741 ccgctggaca gctgcacggt ggccagcagc tcggcatggc gttcgaaaaa gtccggcgtg
     1801 ttggccagga agttggcgat gtcgtcttcg gtgatcgggt tctggatgga gtgttcgctt
     1861 ggattcatgt tcttgtggtt tccgtaaatc agtatcggtc agtctacagg tggtcgggaa
     1921 cgtcgatggc ggcttcaaac accgtggtcg ccggcccggt catcagcact ggggcggtcg
     1981 cggcgtccag cccgccctgc cactcgatgg tcagggtgcc gccatgggtc aacacgtcca
     2041 cgcgcgcatc cagcaggccc aagcgtatgc cagcgaccac cgccgcgcaa gccccgctgc
     2101 cacaggccag ggtttcgccg gcgccgcgtt catacacacg cagggcgatc cggctgcgcg
     2161 agatgacctg catgaagccg gcgttgacgc ggcgcgggaa acgcacgtgg ttctcgatca
     2221 acgggccgtc ttgcagcacc ggtgcggtct ccacatcgtc caccagctgc accgcatgcg
     2281 ggttgcccat ggacagaatt gctacaaaaa caggagctgc tagcgcccgc ccctcaaggg
     2341 ccagaggcca tttctgccat gaatgtacgg tttcgggcgt caaaccggtg gcatcaaaag
     2401 gcacccgcgc caggtcgaag atgggcgcac ccatgtccac cgtgacgcgg ccgtccggct
     2461 gcatgcgcgg ctcgatgacg ccggccaggg tccggaccct gaccacgtcc ttgtcggtga
     2521 ggccctgctc gcgcacaaag cgcaagaagc agcgtgcacc gttgccgcat tgctcgacct
     2581 cgccgccgtc ggcgttgtgg atcacgtatt cgaaatctat accttcgccc ggcgcaggcc
     2641 gcaccgacag gatctggtcg gcgcccacgc caaagtggcg gtccgccaga aagcggtact
     2701 ggcctggcgt caggttcagg cgctgctgcg tctcgttgag catgacaaaa tcgttgcccg
     2761 caccctgcat tttggtaaat cggattagca tccgcgcatt atccgctgag gctgcgccgc
     2821 cctccttccc atccttcccc cacttgcaca acttccggaa agacccacca tgcgcattcc
     2881 cgactggaca cccgccctca agccccaacc ccaggacctc gtcgaggcca tcctgaaacg
     2941 ccgcggcggc accctgatca acctcgacaa ggccctgctc tggagcgagc cgctggcgcg
     3001 cgcctggaac gtctacctgg gcgcggtacg caccggcctg ccgaccagcc gcaagctgcg
     3061 cgagctgggc atctgcacgg tcgcgctgct gaccggtgcc gcctatgaat acaaacacca
     3121 cgcgccggac tttctggccg ccggcggcac ccaggccgaa ctcgacgccc tcaatagtct
     3181 ggtgaaagcc gatgcccgcg cctcggcagc gcatccggcg ctgggcgcgg tcgagcaact
     3241 ggtgattcgt tacgcggcgc agatgacgct ggacgtcaag gtcagcgacg acgtgttcga
     3301 ggccttgcgc aaacatttcg atacgactga gctggttgaa ctgacaagcg cgatcgccac
     3361 ctacaacatg gtggcgcgct ttctggtggc gctgggcgtc tcgccagagg agcatgaata
     3421 gctgaaggtg caacaggaca tccgtttttc atggcgcgtc ctacaccccc gcatccagaa
     3481 aaggcgacat gaaaaccctg cgaacatcct cccgtttttt tgcttcttcc cgtccccggt
     3541 tggctaccct ggtgggaatc ctggcgctgg cggcgggtac tgccgcgcct tcggccatcg
     3601 ctcaagccat gcccgcaggc ggcagtccgg cgcaagccgc gcccgatgac gcagaactca
     3661 atcagcgctt tgcacgctgg atcgccgact tcagcaccag cgcacgcgca gcaggcatca
     3721 ccgaggagac gctgcagagc gccttcgatg gcgttcgctt cattccgcgc gtgaccgagc
     3781 tggacagcgc ccagcccgag ttcacccgcg ccgtctggga ctacctcgac agcgcggtgt
     3841 cgccgcagcg catcgtgcgc ggccaggaca ggctgctgca atcgcgaccc gccatcgatg
     3901 ccgccgcagc gcgctacggc gttccggcag aaatcctggt ggcgatctgg ggcatggaga
     3961 gcaacttcgg cagcaatacc ggcgacatcc cgaccatcga cgccctggca acgctcggct
     4021 tcgaaggccg gcgcgaggcc tgggcgcgta cccagctgct cgcagcactc aagattcttc
     4081 aaaaccggga catagaccgc gcacagatgc tgggttcgtg ggctggcgcg atgggccaga
     4141 cgcagttcct gccaacgaac tttctggcct atgcggtcga tgccgatggc gacagccggc
     4201 gcgacatctg gggcagcctg cccgatgtga tggcctcgac cgccaacttc ctggcccgtt
     4261 ccggctggca ggccggccag ccctggggcc tggaggtgcg cctgccaccg ggattcgacc
     4321 tcgcgctcgc cgatgccgac gtgcgccagc cggcctcggc ctgggcggcc cagggcgtgc
     4381 agtccatgga cggcgcgccg ctgcccgacc tggccgacag tgcccttctt ctgccggccg
     4441 gcgcgcgtgg gccggccttc ctggtcggcc ccaacttccg caccgtcctg cgctacaaca
     4501 actcgaccag ttatgcgctc gccatcggcc tgctggggca aggactggcc ggtggccccg
     4561 cagtgcaggc cgcctggccg cgtgatctgc aaccgctaac gcgcagccag ttgctggcgc
     4621 tgcagacggc gctgaacgcg cgcggcttcg acagcggcac gcccgacggc acgatgggtc
     4681 cggccacgcg gcgcggcata cgtctctacc agcgtagcct tagcctgccg gcggatgggt
     4741 atccgacttt ggagttgctg caaggcctgc agtgacggcg gcagagccca cgctgaatac
     4801 tggatacacg actgcaggcg cttcccacac agcccatcta tcggtcagtt tcttgccttg
     4861 gtgggggagt ccatatacga cctgttaacc gtcattcgcc accacctcaa tgatttgttc
     4921 gccagcgagc agcactgcag ctaagagcga ctcgacgtct ccaggagtga caacgagctg
     4981 ggttcccact ggctgagggg ccccggtttt gtccaagtac tggcggtcca cgataccccg
     5041 tcggtgcacg atgaggttgc gcttttggaa cagtgtccaa agccgctcgt cgcctaacgc
     5101 acgcgccatt tccgcagcat caggaaaaag ggctccatag gtctcgcgaa tagtctgaat
     5161 gtcgtcgagg tcagcccgct gagcaaacag cgtgcccatg ctagaggata ggtcatatga
     5221 gtagctcgca agggtctgcc aatcgacctt ctccccgctg aaacgcttcc tcgtattggg
     5281 atgcgcgaag agcctctcac tcagttgcgg cttctcgttc agaagccgca cgaagatatc
     5341 gcgagccagc acttcgaacg cactccacat caggacaatg ccctgccggg tgagttcgcg
     5401 ggcggtagca agcgattcct cgctggacgc taggcggtgc agccttgcaa gtagctgttc
     5461 gcgaagcacg gcgccgtcgt cttcggccaa gaacttcttt agcttggctt tggcctttgc
     5521 gagagcaacg tcttcgcgtt ccgcttcgtt ctcgtgcccc aaggagcgaa tccgttcggc
     5581 catcagaatt ctctgccagt gcagcccatg cacttccgtg tacgcgaagt cgaatggcat
     5641 gctcaaggtc cgcatcatcc ccttcaggct atcgaggaaa gagagcgacg tgatcctgtg
     5701 cggctcagtg atccggcctg tgaccgtcag caatgacgct gcagtgtgcg agaaatcctc
     5761 cggcaccagg aacacctcgc gcacgaactc agcctgatcc tttgatatct cgtccatgac
     5821 ggctaccgtt ggaggccagg cggggccgta ggccgtcgcc tctgggccga ccagttaaat
     5881 tgcttgcgcg ccaacatgac ttggaaagac ccgccgccaa aatacatacg gtcgcaaatg
     5941 aggtcaattt gagaaacttt catttggctc ttttccgtcc ggcgatgact gctaccgaac
     6001 ccacgaccac aagccaacca gctaccgaaa atacgataag tgagagtggc gcgttgtgtc
     6061 cacaccagac gcccagcaac tgccaaggcc cagcgcaacc cacggccaac acgacaaacg
     6121 caaaaatggg accgctaaac agcaacccca atatcctgat ggcgcctaca accatgattg
     6181 caatttaacg ttgaaggcaa ccggaccgca acctctgcgg cgaagccgct tcctgcagtt
     6241 gtggttccgt gttggccgac cagttaaaca acatgctcat tgcttcgcct ccccttggca
     6301 gaattccagc aaccacagcg cgtggccccg agcgggctcc acagctgttg cgatataact
     6361 tgcaagcgat gccctcactt ggtcgggaaa tagagcggga atttccaaca gcgcggccgt
     6421 aaatccatcg ttgtcgtgac cctcgtcgaa gccgcgctga tacgctttga gcagttcagc
     6481 aagtacagtc ggaccggcca attcggcgag cggccaagca gcggcttcgc gcgcaaagaa
     6541 atcgccggtc tctaacaatg ccagcagctt cggaacgtcg tcggcgtcag cttccgcatg
     6601 cagcctttca gctgccgcta cacacgtctc aacgctttgt gcgtctaagg cagacatgat
     6661 ctcggtccaa gcggtggctt ccatgctgtt taactttgat atatcccgca aaactgcgga
     6721 ataacatggc gaccagtcag ataacatggc acctgcttgt tgaaaaacac gtttgaattc
     6781 atgaagttac atcaatagct gtatccatgc gcaggtatat tcaatccgac aaagccgccg
     6841 accctgtttg acttgcctct gcttgccaga ccaggtaccc gaccgagcgc acgcctgatt
     6901 tcactatagc cttcaaaccg ataaaaaccg tgtgtacccg acgcacatct catccccatg
     6961 catggtcggg gcgacaccat gcagcctcca cgcaacatgg gtaactttgg tcttacagtc
     7021 aggcgtgccc tttcatccgg cacagacgta aaaaaaggcc cgcaggccct tttcttattt
     7081 gcaccaagcc ctcaatccgc cttgatcccg ttgtccttca ccaccttggc ccaagtgtcg
     7141 atctgcttgt cgatgaagac acgggctgac tcggaagaac cgccgacgat ctcgatgccc
     7201 tgggcgctga gcttcttggc gatctcggga tttttcagcg ccttgttgac ttcctcgttc
     7261 atgcgtttga ggacctcggg cggtgtcttg gccggagcca ggatggccca ccaggcgggt
     7321 gcgtcaaagc cggcaaaacc gctttcggcc actgtcggta cgttcggcag ttcaggtgcg
     7381 cgtttgctgg tcgtcacggc cagcggacgc aggcggccgc tgtcgatgtg cggcttggtc
     7441 acaaacaccg aaccgatggc cagcggcaca tggccggcca cggcgtcgct catcagcggg
     7501 ccgccgcctt tgtaggggac gtgctgccag tcgatgccgc cacttttgga gagaagcaac
     7561 atggccaggt ggcccaggct gccggaaccg atgctgccgt agttcacatt ctttttggcc
     7621 ttggccgcag ccaccacatc gccaaaggtc ttgtactcgg agcctgaaaa agtcgcgagc
     7681 accatggccg aggtgccgat cagcgtgacc ggtgccaggt ccttgcgcgt gtcgaacggc
     7741 aggccgggaa tcaggctggg gttgacgccg tgggtgtcga acacaaaggc aaaggtgtag
     7801 ccgtcggccg gcgcggcagc cacggcggcg gtgccgatgg agcccgaggc gccgcccttg
     7861 ttttcgacaa tgatgttctg gccgagctgc tgcgacagcg gcacggcgag aatgcgcgcg
     7921 acctggtcga cagagccgcc gggcgggaat acggcgacaa acttgattgg ttgtttcgcg
     7981 ggccacgttt gggccatcgc gctcatcgag gtggcgcaca gcgtggcggc cgtcatggcc
     8041 agggcggttc tggaaatcca ctgcttcatg attcacatcc tgcaaataaa gtgatggcca
     8101 gtatcctcac gctgccgcca gaacgccatt gcggttaaca cttagcatgc taccaatcgt
     8161 ggcccgccat gggctgccac agccgccgcc cgccgtttca ctgcaaaatc aggccatggt
     8221 ccgacccacc caactccttt acccgcagcg cgaacccgcg cccacccgcc ctgccgccac
     8281 cgtgctgctg ctgcgcgata gcgccagggg catcgaaatc ctgatgacac ggcgttcgat
     8341 gacggccagt tttgcgccgg gcgcctacgt gttccccggc ggcggcatcg acgcggccga
     8401 tgcggcagcg cacgcgcaat ccacccgccg cgcgacgcag accgacctgc acctgacgca
     8461 ggcgattgcc gccatccgcg aaagttttga agaactcggc gtgctgctgg cccgccacgc
     8521 tgatggcagc cacgccacca ccgccgacat tgcagcgctg gaccgcaagg cgccgtttgc
     8581 cgcgcagtgc cgcgaacgcg gcctgacact ggccggcgac caggtgtttg tgctggcgca
     8641 ctggatcacc gaccgcgacc tgccgcgccg ttttgacgtg ccctttctgg tggcgcgcat
     8701 gcccgaaggc caggagccag tggccgacga aagcgaacag ttcgagccgg tctgggtgcg
     8761 gccggccgat gccctggcgc ggcatgaagc cggcagcttt ttcatcatct tcccgaccat
     8821 acgcacgctg cagcgcctgc agcatttcga gacggtggac gccgtgctgc aggcctgcgc
     8881 cgcgtccgag cagccgctgt ggacctcgtg cccgcgcgcc ggttttctgg ctggcgccga
     8941 agcgcgctac atggagcacg aggggcctta tggcgaactg gccctgacca gccccgacgg
     9001 gcagatggtg cacacgctgg actggcagac cgcgcagccg gtggccctgc tgaaaaacct
     9061 gatgcgcctg accgcgccca accccggcgt gatgaccggg ccgggtacca acagctacct
     9121 ggtcggcgac ccggccaccg gctacatcgc catcgacccg ggcccggccg acaccgacca
     9181 cctgcagcgt ttgtggcgcg cagcgggcgg caacatccgc atgattgtgt gtacccactc
     9241 acacgccgac cattcgccgg gcgccaaacc gctgcaggcc atgtgcagca acacgccccc
     9301 catcctgggc ctgccgtcag cgccgacggc gcgtgcggcc agcaccttca cccctgaccg
     9361 ggcgctacga aatcaggagc tgctcgcgct tgcaggacaa gggcccgaag gcgaaatgat
     9421 gcataccctg aaggtcatcc acacccccgg ccacgccgcc aaccacctgt gcctggtgat
     9481 gctcgaagac ggcatcctgt tcagcggcga ccacatcctc aacggcagca cc
//
LOCUS       DAIDFG010000097         9444 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_97_length_9444_cov_17.672915, whole genome shotgun sequence.
ACCESSION   DAIDFG010000097 DAIDFG010000000
VERSION     DAIDFG010000097.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 9444)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9444)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9444
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97_length_9444_cov_17.672915"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1562 a         3061 c         3188 g         1633 t
ORIGIN      
        1 gcggctcctt gtcttcctgg ttgtccttgt tgtaggccag cggctggcct ttcatcaggg
       61 tgatcaaacc catcaggtgg ccgaccacgc ggccggtctt gccgcgtgcg agttcgggca
      121 cgtcggggtt tttcttctgc ggcatgatgg acgagccggt ggtgaagcgg tcggcaattt
      181 caatgaagcc gaagttctgg ctcatccaca gaatcagctc ctcgctcatg cggctgatgt
      241 ggaccatggc cagcgaggcg gcggccgtga attcaatcgc gaagtcgcgg tcgctgaccg
      301 cgtccaggct gttctggcag acctgcggct ggcccttttc atcgaccatg cccaaggtgg
      361 tggcgacacg ttcgcggtcc agcgggtagc tggtgccggc cagtgcggcg gcgcccagcg
      421 gcaggcggtt cacgcgtttg cgcacgtcct gcaggcgctc ggcgtcgcgc gcaaacatct
      481 ccacgtaggc cagcatgtgg tggccaaagc tcaccggctg cgccacctgc agatgcgtga
      541 aaccgggcag gatgacctcg acgtttttct cggcaatatc gaccagcgcc ttttgcaggt
      601 cggtcagcag cgcatcaatc aggtcgatct cgccgcgcag ccacaggcgc acgtcggtgg
      661 cgacctggtc gttgcgagag cggccggtgt gcaggcgttt gccggcgtcg ccgacgagct
      721 gggtcaggcg cgcttcgata ttgaggtgca cgtcttcgag gtccagcttc cattcaaagg
      781 cgccggattc gatttcggct gtgatctgcg ccatgccttt ctggatggcc gcatggtcgg
      841 cggcgccgag gattttttgc gcggccagca tctcggcgtg cgccagcgaa ccggtgatgt
      901 cggcttgagc cagccgcttg tcaaaaaaca cgctggcggt gtaacgcttg accaggtcgc
      961 tcatgggttc cgagaacagg gcggaccagg cttgggattt tttgtcgagt tggctcacgg
     1021 gaggctttct gtggaggacc gtctggcggc agcgatggcg tgtcgcggcg acaacattat
     1081 tgggggaagt acgggtttga cgccggtttc aagcgggttg ccggtgcaat actagggcct
     1141 gattttatct gcgtgcagga aagccccgtc aagcggggcg cccgggacct ccgggccggc
     1201 ccgcgcgcag aaccggacac ggaacaagcg agcctttttc atgcagttca agcccgacct
     1261 ttcgtccttc ggcgagtcga ccgtgctggg tggttacagc cagcccgggg atgcgcctgc
     1321 gcccctgccg ccccgtgtca atgtgtttga cgcctgcaat gtcggtgtgg tgctgcgcgc
     1381 cgtgttgttt gtcgaggcgg tggtgggcgt cggcgcgatg ttcggcgccg gcgtctttgt
     1441 cgactggctg ctgcgttttt cgctgctgac cggcggcgtg ttgcccgcca cgctggcctg
     1501 gctgatcgcc gcctgtgtgc tcaaggggcg gctggcccgg ctgggcacgg cgcagcagtg
     1561 gatggctggc gtgtcgcttg gcgcgctggc gggcctgtat ggctgcggcc tgctggtgct
     1621 gctgaacctg cttgatccgg cgccctggtg ggccagcgct gcggccggcg ccctgctgtc
     1681 ggcggtgctg gtggccgggc tggtctggcg cgccaaggcg cgcacaccgg cggccacggc
     1741 ggcgcgcctg gccgagctgc agtcgcgcat ccggccgcat ttcctgttca acacgctcaa
     1801 cagcgccatc gccctggtgc gcgctgaacc ggccaaggcc gaagccgtgc tggaagacct
     1861 cagcgagctg tttcgccatg cgctggccga ccagaccgag tcggtcacgc tggggcagga
     1921 aattgcactg gcgcagcgtt acctggccat cgagcagatc cgttttggcg agcgcctgca
     1981 ggtgcagtgg agtctggacc ccggggccga cccggccaaa ttgccgccgc tgctgctgca
     2041 gccgctggtg gaaaatgcgg tgcgccacgg cgtggagccc agcgcccagg gcgcgcagat
     2101 caagatcacc accgaacggc gcggcggcgt ggtggtgctc aaggtgacca attccacccc
     2161 ggccggcgtc ggcgagcggg ggcacgggct ggcgctggcc aatgtgcgcg agcgcctggc
     2221 gctgctgcat gatgtgcagg cgcaattcaa gaccgtgttc aaggacggtg tttttcaagt
     2281 gaggctggag attccgctat gacaggcaag ttgagggcgc tgattgtgga tgacgaggcg
     2341 ctggcgcgtt cgcgcctgcg cacgctgctg ggcgactgca cggcgccgcc cgcctgcgtg
     2401 gccgccgaag ccgcgcatgc ggcgcaggcc atggacgcgc tgcgccgcga aacctttgat
     2461 gtggtcctgc tcgacatccg catgcccggc gccgacggca tcgccctggc gcagaccatt
     2521 gcccgtttgc cgcagccgcc ggcggtggtg tttgtcaccg cgcattccga acacgcggtg
     2581 caggccttcg atctcgaggc ggtggactac ctgaccaagc ccgtgcggct cgagcgcctg
     2641 cagcaggcgc tacaaaaagt agagcggctt gcgcatacgg gtaaagggct ggaggccgat
     2701 tcgaatggtg attttctgat catccaggac cgcggccgga ccgagcgtgt gccgctggcc
     2761 gaggtgctgt acctcaaggc cgaactcaag tacatcacgg tgcgcacggc gggcaaaagc
     2821 tacatcctcg acggcagcct caacgaactg gaggaaaaat acccccagca gttcatgcgc
     2881 atccaccgta acgcgctggt ggcccgccgc gcggtgcgcg cgcttgaaaa acactttgat
     2941 cccgaagaag gggagggctg ggccgtgcgg ctcaacggca tcgacgagtt gctggcggtg
     3001 tcgcggcgcc agctcagcgc ggtgcgcgag gccatcgccg cttgagcacc ccacctggct
     3061 tgaaacccta cctgttctgg cgacggcgtc ctgcaccgcc cgcccgcctg ccgcggtgaa
     3121 atggggccgg tacaggaatg gagacttcgt cccagggcgg ttttgccagt gacaccccgc
     3181 ggagcgcccc acaaggaaca ccaatgagcg aagaacacgt gcccgaaata ctgccggcag
     3241 aacctgcggc gcctgccgat cccgaggtga cgaccttccc ggagccggat cgcctgctgc
     3301 tgcacatgcc ggtggacatc cgcaacatgt cgctgatggt gcttgccggc ctggccattt
     3361 tgttcgtgct gcactgggcc aaggcagtgt tcattccggt gatgctcggc gtgttggcca
     3421 gttatgcgct ctcgccggtg gtcaactggc tggaactcaa gcgtgtgccg cgctggctca
     3481 gttctgccgt gctgctgctg gcgattttcg gtgccatcgc ctcgaccacc tggtccctca
     3541 gggacaacgc ggcgcaactg gtggagtcgc tgccggtggc cgcgcaaaaa ttccgcaacg
     3601 ccatgaaggt caaggccgga ggtgcggaca gcaccctgga cagcgtgcag aaagccgcat
     3661 cgcaaatcga gcaggccacc caggaaggcg ccagaccggg caacacccgt ggcgccatgc
     3721 gggtggtggt cgagcgggca agtttcaacg tcaaggacta tctctggacc ggcaccatcg
     3781 gcctggtcac gctggtcggt caggccgttg cggtcatttt cctgacctac ttcctgctgc
     3841 tctcgggcga cagcttccgg cgcaagctgc tcaagctggc cggccccagc ttcagcaaga
     3901 aaaaagtgac gctgcaggcc ctgcatgaaa tcaccggcca gatccagcgc tacctgcagg
     3961 tgcaactgct caccagcgcg ctggtcgggg tgctgacctg gctggccctg ctggccatcg
     4021 gactggagaa tgcggcggtc tggggcattg tcgccttcgt gctcaacttc gtgccttatg
     4081 tgggctcgct gatcaccgcg gccgccacgg cgctggtcgg tttcctgcag ttcggcacgc
     4141 tgaacatggc gctggtggtc ggcggcgcct cgctggtcat ccacaccatc gtcggcaacc
     4201 tggtcacgcc ctggctcacc agccgggcca gccgcctgag cccggtggcg gtttttgtcg
     4261 gccttctggc ctggggctgg ctctggggcg tgtggggact gctgctgggc gttcccatca
     4321 tgatgatggt caagtcggtc tgcgaccggg tggacgacct caagcccctg ggtgagtttc
     4381 tgggcccctg agtcgggccg gatctttttt aaccattttg gcctccagcc ctttcataga
     4441 ctgcgcaaag tgctattgat ttgatagtga atgcatcgcc gccctgccgc ccggattgtg
     4501 cagtctgcct gcgtccgcaa agcgcctgca tctgtgcctg ggtcacgccg gtggcgcatg
     4561 aggtcgaggt gctgatcctg cagcacccgc tggaggtcga tcaccccaag ggcagcgcgc
     4621 ggctgctgca cctgagcctg ccgcgcagcc gcctggtgac gggcgagggg tttgccgacg
     4681 cggccctgtt ggcagcacct ttcgaggcgg ccggggccgt ggcgggcact ccccggcaca
     4741 accttctttt gtacccggac atgccgcagg atcaggcgct gggcgaaccg gccccgcccg
     4801 ccttgccggc cgggtggctg cgcgagcccg ccaggctgcg cctgatcgtg ctcgacggca
     4861 cctggcgcaa gagccgcaag atgctttacc tcagcccgcc gctgcaggcc ttgccgcgcc
     4921 tgtcgctgca gggcttgccg ccgtcgcgtt acagcatccg cgaagcgcac cggcccgacc
     4981 agctgtcgac gctggaggca acctgtgcgg cgctggcgca gcttgaaggc gatgccgaga
     5041 agtttgaacc gctgttgacg gcctttgacg gtttcgtggc gcagcagctc ggctaccttt
     5101 ctagtcaccc aggtcgataa cacccgccag cggcacaccg gccggtgccg catagtcagc
     5161 gaccacgaag gcgcgcaggg ggtgggctgc ggcatcttcc agcagtgcca gaacaaaacc
     5221 gatcgacgcc ttgtccagcg cggcaaacgg catgtccagc agggtgagtg ccgcacccga
     5281 ggcaaaggcc gccgccagcc agaccttgcg ctttgagccg gtagacaaca tgaaaatctg
     5341 cttggtcagc tccggcgcca gcgccaggcc gtccacacag gtggccagca cgccatcgtc
     5401 aaagccggca taggctcggc gctgcacatc gaagtagtgc gccggtgtca tctggtcgaa
     5461 tgcctccgtg cgtggctctg tccagaacac ctgccggcgg tagtccgcag cctgatcctg
     5521 cagggacacg ctgttgatct tcaaggagcc tgctgcagcc ggtagttgcc ctgccagcag
     5581 acgcagcagc gtggtcttgc cacgcccgtc gccgccatgc acgaaggtga cgcccggtga
     5641 aatggcaacc gacaagccgg cgaacagttc gcgctcggga taacaaaaac gcaggccttc
     5701 cgcctggaga accatggggg aggccgccgc acgcggggag ggaaggcttt gacgattcgt
     5761 ctggtcaggc tgtgtcatgc ctgtattgga catgatccgc catggcccgc atgccggtgc
     5821 caaccaccgt ttccatcggc gcgtgccaaa ggccagccgg gtcagggttg gtgggctgtg
     5881 caaaaaaaag atcccggccc ttcatttttc ctgcccgcag ccgttatgca tgaaggcacg
     5941 cgtcactgcg cgcctgccgt gctcatccgg cattccggat tcagaaaata aaggaacagt
     6001 catcatgaaa aaacttctcc aggtacttgt gttcggtttg gcggccctga gcttcaacat
     6061 gacggccatg gctgccggcg aacaggggtc ggccgcagaa gccaccgcgc tcgtcaagaa
     6121 agccattgcc tacatcaaag ccaacggcag ggaaaaggcc ttcgccgaat tcagcaatcc
     6181 gaacggcccg ttcagggatc gcgaccttta catgaccacc gttgacacgg aaggaaagat
     6241 gctggcacat ggcgtcaatc ccaagctcgt cggcaagtcg ctgatcgaac tcaaggacat
     6301 cgacggcaaa tatttcgtca aggaatacgt cgacctggcg aaaaccaagg gcaatggctg
     6361 ggtcgatttc aaatggccca acccgatcac caaagtcgtc cagcaaaaat ccatgtatgt
     6421 cgagaaaatg gacgacctgg tcgttggctg cggcatttac aaataaagcg gctccggcgt
     6481 ggtctgcgtg acaagtccgt ctcgcgccag cactgcgaat gaaaaaggcc gttggccctt
     6541 atccggcaag cgcaacatgc tcccaaaaaa atagcattcc agttgccgct cgcctcaggg
     6601 gccggcctgc accagcaggc tggccgacat cggcaggtcc gccagccgca ctacttcgcc
     6661 gggcgccagc acacggtccg gcttgaagct ttcttcctgc gccacatcgg cacgctgcca
     6721 gcgcaaggtc accacgcgtc cgtcgagccg cagcgtgatg ccaaacgatg gccagtgcgc
     6781 gggcacacgc ggcgtcagcg acagctcgcc ctggcgcacc gacaggccca gcagggtttc
     6841 cagcgccgcg cggtgcagcc aggccgccga gccggtgtac cagctccagc cgccgcgccc
     6901 gacatagggc ggggcgctgt agatgtcgcc ggccagcaca tagggttcaa tttcataggc
     6961 cgggccgcgg gtggggtggg cgctgcggtg cgccgggctc agggcttcaa agctgcgcca
     7021 ggcggtttcg atgtcgccgc ccagggcctg cgccatcatg gcccagaccc cggcgtggga
     7081 gtactggccg ccgttttcgc gcacgccggg cgggtaggcc tggatatagc ccgggttgtt
     7141 ttcagaatgc tgcagcggcg ggtccagcag gcgcagcaac ccggcgtcgg ggtcggtcag
     7201 ctgctgcttg agggccagca tggccggcac ggtgaaggcc tcggtggagg cgccggacag
     7261 cacggaccag gcctgcgcga tcaggtcgat ccggcattcg ctgttggcgc tggagccgag
     7321 cggcgagcca ttgtcaaaaa acgcgcggcg gaaccaggcg ccgtcccagc cggcgtcgtg
     7381 cagcgcggcg atccagcctt gccgcgcgtg cagccatgtg tcggcgcgtg cggtctcgcc
     7441 tctggcccgg gccagcggcg caaagtcctg caccacgcgg cacaggaacc agcccagcca
     7501 gacggactcg cccttgccct cgtggccgac acggttcatg ccgtcgttcc agtcgccggt
     7561 gcccatcagc ggcagaccgt gcgtcccgac ggccaggctg cggtcgatgg tgcgcgcgca
     7621 gtgttcgtaa acgctggccg actgcgtgct ggtttgcggc gggtagtagg cgtcttcggc
     7681 gccgtccggg atggccggtc cgtcgatgaa ggccacggat tcgtcgagca gggccaggtc
     7741 gcccgtgatg tcgatgtagt gcgcgcaggc atggggcagc cagagcaggt cgtcggaaaa
     7801 atgcgtgcgc acgccctcgc cgccgggcgc gtgccaccag tgctgcacgt cgccttcggg
     7861 gaactggcgc gaggcattga ggacaatctg cgcacgcagc cgtgccgggt cggccaggcc
     7921 cagcgccatg gcgtcctgca gctggtcacg aaagccgaag gcgccaccgg cctggtagaa
     7981 gccggccttg gaccacagcc ggcagaccac ggtctggtag atcagccagc ggttgaccag
     8041 cgcgtcgaac agcgggtcgg gcgtgctgac ctcgaccttg cccagcagtt gcgaccacca
     8101 ggactgaacc gcctgcaggg cctggccggt gtcccgctgt gcccactgcc tggccagttg
     8161 ctcggcgccg gccccgtcgg ccgcatgacc gaggaggaag ctgaacgtca ggtcttcggt
     8221 gcccgccagc gagaactcgg tggcgagggc tgcgcacggg tcgacggcgc cgccgctgcg
     8281 ccgtccgagg atggcgggca gcaccagttg gcccagcgtg tcgaaaaatt cgctgcggtc
     8341 gcaggtccat tgcgtgccgc ctgccgcacc cgacagggcc aggaaggccg tgctgccgcc
     8401 aaaaccatcg cggctttcac gctgctgggc aaacacggcc gcgcgttcgg cgccgttcca
     8461 ggtgtgaagc gtgcgccggc tgcccctggc ttcgcccatc tgccactcga ccatggccac
     8521 ggcgcgcagc cgccgccggg cgctgccttc gtccagccgc acgcgcacct ggaccacctt
     8581 gaccgcttca tgcaggtcgg caaaaaaagt ggtctccagc tgcaggccgg cgtgccggcc
     8641 ctcaaacacc gaatacccct ggccatggcg cacgtgccag ctgctgccgg gtgcggccag
     8701 cagcgaaggc gccagcgcga acacttcgcc gctgtccagg tcctgcagca gccagtgctc
     8761 aaacgcgggg tcacgcaccg ggtcgttcga ccagggggtg atctgatgca tgcggctgtt
     8821 gccggcccag ctgtagccac tgccggtttc cgacacctgg aaaccaaaag acggattgcc
     8881 gatgacgttg acccagggcc ggggaaggcg ctggccgcca gccagctcga accggtactc
     8941 gccgctggcg gcatcgaagc ggcccggggc aagcccgtct gccacggcag gcgcgtggcc
     9001 ggccagcgcg gttggtgcca cggtgccgga ccgcctcggc aaccagcttt cgcgcagggc
     9061 cgcgacctgc atttccagcg gccgtccgtc ggcgttgagc accacccgtg ccagcccggt
     9121 cagcgcactt ttttcgccgg aagacatttc ctgctcgcgc agcaggaaaa agcccgcagc
     9181 cgacgatgcg gcttcatcgc gcgggaaact gtgttgcacc gcctgcgtga gacggtcgcg
     9241 cagcgccagg atgtcgcgct gcagcggcat gaggtaggag ttgacttcgc tgttgaccac
     9301 caccacgtcg accgccagcc cgccgaaggc ccaccagggc tgggcgcgca gcagtgcatg
     9361 caccagcggc aggccgctgg gtgaatggat gcgcagcagc acgatgggct tgtcgccgga
     9421 gatggcgaag cgccagagct ggcg
//
LOCUS       DAIDFG010000098         9421 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_98_length_9421_cov_18.955904, whole genome shotgun sequence.
ACCESSION   DAIDFG010000098 DAIDFG010000000
VERSION     DAIDFG010000098.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 9421)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9421)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9421
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98_length_9421_cov_18.955904"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1772 a         3092 c         2982 g         1575 t
ORIGIN      
        1 gagaaagtga gtcgcccgcc ggggcgagac ccggcttgtc tgaagcaccg ccaacgcagg
       61 aggaaaaggc ggcttcgaca ggctcagccc gaacggtgcc tcgaaacgct actaattcaa
      121 tagctgcttg cgcttatggg aaaagggctg cagccacttt tcatccagac agtccacgac
      181 aaccggccgc cacaaaggga cgcaaaggaa cacagaggga tttaccatag ccccatgccc
      241 accacccacc tcctctacct ccacggcttc cgctcctccc cccagtccgc caaagcccgg
      301 caggtcgcag cgtggatgca aggccaccac cccggagtgg tgtggtggtg cccgcaactg
      361 ccgccttcac cgcgggaagc aatggaaaag ctgcgccagg gcatcgccgg ctggccccgt
      421 gacaccatgg cagtgatggg ttcgtcgctg ggcggttttt atgcgacgca tgtggccgaa
      481 acctgcacct gcaaggccgt gttgctcaac ccggcggtga atccggcgcg cgatctggcg
      541 aagtacatcg gcgaacagac cgcctggcac gacccgaacg aacacttttt cttcgagccg
      601 cggtttgtcg acgaactgcg cgcgctggcc cagggacccg tcgcgcgacc ggaaaactat
      661 tttgcggtga tcgccaaggg cgatgaggtg ctggactggc gcgaaatgac cggccgctac
      721 ccgggcgcgc acatccggct gctcgaaggc ggcgaccacg ccatcagcga cttcgagcgc
      781 cacctgccgg aggtgatgga cttcctggaa cttgcctgag gccggcccgg ggcggctgat
      841 tacactagcc agtaccgcag ctcggacgta gcgagatgtt cggtttgcag taccagagta
      901 accaaaggat gtcgttttgt ttgtattgtt tgaagaggcc ggcaagtttc tggccggccg
      961 catcttgtcc gaagcggaca cctcgctgca ggtggagctc gactcgggca aacgcgtcaa
     1021 ggtcaaggcc gccaacgcgc tgctgaaatt cgaaaaaccc gcgccggccg agctggtcgc
     1081 cgccggccag cgcctgagcg cggagatcga gctggacctg gcctgggaat ttgcgagcga
     1141 cgaggaattc ggctttgccg atctggcgcg cgactacttc agcgccgacc cggccaaacc
     1201 ggcctccatc gaacagcagg ccgcggccct gttccgcctg ttcgaggcgc cgcattattt
     1261 ccgccgcgcc ggcaaagggc gctttcgcaa ggcgccggtc gagatcctcc agcaggcgct
     1321 ggcggccatc gagaagaaaa agcaggtcgt cgcgcaaatc agcgcctggg ccgaagaact
     1381 cgtccagggc cagtgcccgg cgccagtgcg cgagcagctc tacaagatcc tgttcaagcc
     1441 cgacaagaac agccccgaat acaaggcggt ggtggaggcc tcgcgctcgg cgcacaaggc
     1501 gccgctcgac ctgctgcagc aatccggcgc gattgcctcg ccctaccagt ttcactggaa
     1561 acgttttctg tttgaaaact tcccgaaagg cacgggtttt ccggccgtgc aggcccccgc
     1621 catcagcgac gagttgccgc ttgcggacgt ccgggcgttt tccatcgacg actccagcac
     1681 caccgagatc gacgacgcgc tgtcggtgca gggcctggcg agtcagggag gcaatggcac
     1741 ggtcacgctg ggcatccaca tcgccgcgcc gggcctcgcg gtgttgccgg gcagtccggt
     1801 ggacgctttg ggacgtgcgc gcctgtccac ggtgtacatg ccgggttaca agctgaccat
     1861 gctgcccgac gaggtggtgc aaagctacac gctgcaggaa gggcgcaact gcccggccct
     1921 gtcgctgtat gtgacgttcg acgaagccac gctggcgatc cgtgagtcgg tgacacggct
     1981 cgagcaggtg cccatcgtga gcaacctgcg ccacgacaag ctcgacggaa ccttcaacga
     2041 agctttcttt gaagcggctc aggccacgcc cccggctggc gttacctggg gcgtggaact
     2101 ggcctttttg caccggctgg ccatgcacct gaaggcccag cgcgaagtgg tgcgcggcaa
     2161 gccggaaaac ttcaaccgac ccgactacaa cttctcgctc gacatcgccc cgggcaagga
     2221 gccggaaggc aatgagacgg tcagcatcag catccgcaaa cgcggcgcgc cgctggacct
     2281 gatcgtcgcc gaggccatga tcctggccaa cagcacctgg ggcgagtggc tggccacgca
     2341 tggcgtgccc ggcatttacc gcagccaggc cagcctgctg ccgggcgtca aggtgcgcat
     2401 gggcaccaaa gccctgccgc atgccggcat cggcgtcaaa agttacgcct gggccacatc
     2461 gccgctgcgc cgctacaccg atctggtcaa ccagtggcag atcatcgcct gcgtcaaaca
     2521 cggccgcacg gccgcgctgg ccgccccgtt caagccgaaa gacgcggaac tgttttccat
     2581 catctcgggc tttgatggtg cctacagcgc ctacaacggt ttccaggccg gcatcgagcg
     2641 ttactggacg ctcaaatacc tgcagcagaa ccagatcacc gagctcacgg cgacagcctt
     2701 caaggacaac ctggtgcgcg ccgacgacct gccgctggtg ttgccggccg ccggtgccca
     2761 ggggctgccg cgcggcgcga aggtgcgcat ccggctgggc gacatcgacg agatcacgct
     2821 cgacatccac agcacggtga ttgaacgcct ggacgccgtg gtggacgaag ccgacaccga
     2881 gcaggtggcc gaggaagaca acgaatcgga aatcgccgcc ggtccgatct ccatcgccgt
     2941 cgatctgaac gagccggccg acgacgcggc cgctgcggca gcttagcccc gataatccag
     3001 tcctgtgaag tcccccagca ccctgcaaat cgccctgggc gtatcgctcg ccgtccatgc
     3061 ggtcctcctg accgtgcgtt ttgtcgatcc ggaacgtttc aaccgcattt ttcaggacac
     3121 gccgctggag gtgatcctcg tcaacgcgaa gtccagcgaa cgcccggacc aggccaaggc
     3181 gatcgcccag gcctcgctgg ccggcggtgg cgaggcggaa aaaggccggg ccaccagccc
     3241 gctgccgcct tcggcgttga ccgaactcgg cgacgccagc gaagatgcgc agcgcaaggt
     3301 cgagaccatg caggagcagc agaccatgct gctggcacag atcaagaagc agctcgcggc
     3361 catgccgccg cctgacccga aagtggcgtc cgccgacccg gcccagaccg agcgtgagga
     3421 aaagcgcaaa cagctgatca agatcctggc cgaaatcgaa cggcgcatca acgaagaaaa
     3481 cgcgcgcccc aaaaagcgct acatcagccc cgccacccgc gaggaggtgt atgccgtgta
     3541 ctacgacacg ctgcgccgca agatcgagga aaagggcacg gtaaatttcc cggaaatggc
     3601 cggcaagaag ctgtatggtg aactcaccat gattctcacc gtgaaccacg acgggcgcgt
     3661 cctgtccacc gaagtggccg aaacctcggg caacctgacg ctggaccgtc gcgcgcaaag
     3721 cattgcgcgc agcaccggcc catttgcccg tttcagcgag gccatgcgcc gcaaggccga
     3781 ccagatcgtg gtggtctcgc gtttcagatt cactcgcgac gagacactgg aaaccaggct
     3841 caccgagcga tgagcgtctc catggatcac tatggcgtcc tcggcaaccc ggtggaacac
     3901 agcaagtcgc ccgccatcca tgcgcgtttt gccgagctga ccggccaggc catggattac
     3961 cgcaagctgc tggcaccatt ggaccacttc gccgagacgc tgcagcaatg gatcaccagt
     4021 ggcggacgcg gctgcaacgt caccgtgccc ttcaagttcg aggctttcca gctggccacc
     4081 agcgccaccg aacgcgccca gctggcgcag gccgccaaca cgctgaagtt cgacgccggc
     4141 cgcatcgaag ccgacaacac cgacggcatt ggcctggtca atgacatcca gaacaatgca
     4201 ggcgtccggc tcgccggtcg tgacctgctg ctgcttggcg ccggtggcgc cgcagcgggc
     4261 gtgctggggc cgctgctggc ggccgggccg cgccgcctgg tggtcgccaa ccgcacacac
     4321 gcgcgcgcgg ccctgctggt ggcgcgccac accagccatc cctcgctgca gcaactgcta
     4381 caaaaaacag agctgacagc gcaggagctg caagggctgg aggccgattt tgatgtgatt
     4441 gtcaacgcca cggcaagcag cctcagcggc gcggccatca cgctggcgcc acgcctgctc
     4501 aagcccggcg cgctggccta cgacatgatg tacggcgcgg ctgcccaggg gtttaccgac
     4561 tgggcacggg cgcacggcgc ggtgccgcgc gacggtctcg gcatgctggt ggagcaggcc
     4621 gccgagtcct tcctgttctg gcgcggcgtg cgcccgcctt ctgcccaggt tctccaggaa
     4681 atgcagcaac agcaaaaggc cccgccatga cagccttgct gcgctggatc ctgctggtgc
     4741 tgctggccgc cgtggcgctg cagctttatt ttgtcggccg catcgcgctg atggccgtgg
     4801 tcgatccgca gtccaccgcc tttcagcgct ccgaagcgta ccgcctggcc acggaaaagg
     4861 gcgagctcaa atggcgccag cagtgggtgc cttatgccga gttgtctgcc aacctcaagc
     4921 gtgcggtcat cgcgtccgaa gacgccacct ttgtcgagca tgaaggggtg gacctggaag
     4981 ccctcgaaaa ggcctgggag aaaaacaccc aggccgagca gcgcgccgaa aaggccgccg
     5041 gcaggctgtc cagccgcgcc gcgccggcgg gcgctgccag ggccgccaaa ccacccaaaa
     5101 ttgtgggcgg ctccaccatc acccagcagc tggccaaaaa cctgtttctc tcgggcgaac
     5161 gcaccctgat gcgcaaaggg caggaactgg tgctgaccct gctgctcgaa gccctgctga
     5221 gcaagcagcg cattctggaa atctacctca acaatgttga atggggcgaa ggcgtgttcg
     5281 gcgccgaagc cgcggcccag cactatttcc gcaagcccgc cgccaaactc agcgcctacg
     5341 aggccgcgcg cctggccgtc atgctgccgc gtcccaagta cttcgagacg cgtccgggct
     5401 cgggctacct cgcgtcacgc gcttccacca tcgccgcgcg catgggaggg gtggaggtgc
     5461 cgtgaacata tccgcaaaaa tcatgagcct gtttctcctg ggcctgatcc gggcgctgac
     5521 aggcgcacag gcacgctggc agggttgccc gcccaaggcc gaacagcgca tttatttcgc
     5581 caaccaccag agccacgccg atctggtgtt gatctgggcc gccctgccca gcgagctgcg
     5641 cagcatcaca cggcccatcg ccgccaagga ctactggacc aaaacgccgt tcaagcagtg
     5701 gctcaccacg gccgtgttca acgcgatcta cgtggaccgc gccaagaccg gcgaccagga
     5761 cccgctggag ccgctgatcg atgcgctcga aagcggcgac tccatcatcc tgtttccgga
     5821 gggtacgcgc ggccaccagg aagagccgca ggccttcaag tcgggcctgt acaacctcgc
     5881 gcagaaattt ccgcaggtgg tgctggtgcc agcgtggatc aacaacgtgc agcgcgtcat
     5941 gcccaagggc gaagtggtgc ccgtgcccat cctgtgctcg gtcaccttcg gtgcgcccat
     6001 ggcgttgcag gaaggagagg accgcggcgc cttcctggcg cgtgcgcgtg atgccgtgct
     6061 ggcgctgagg gaaagctgat gagcagcttt ctgcgcaacc tgagcccttc ccatcaagtg
     6121 ggcctgctgt ttgtcgtggt gttcggcctg ctgatcgtgg ccggcatcgc ggcgctcatc
     6181 atgtcactgc gcgacagcgg cgaggacgag gtccgaacgc aggacatcaa ggagttcagc
     6241 agcctgctca acacctcgtg gatcatgtgt accgtgttct gggtcggctg ggccctgggc
     6301 gagacgatgg ccaccttgct gtttgcactg gtggcttttt tcgccctgcg cgaattcatc
     6361 accctgtcgc cgacccgccg cggcgaccac cgcagcctgg tgctggcctt ctttgtggtg
     6421 ctgccggtcc agttctggct ggtcatgacc gagcgctttg acctggtaac cgtgttcatc
     6481 ccggtgtatg tgttcctggc gatcccggtg gtcagcgccc tggccaacga cccgcagcgc
     6541 tttctggagc gcaatgccaa gctgcagtgg ggcatcatgg tctgtgtcta tggcatcagc
     6601 catgtgccgg ccctgctgct gctggacttt ccgggctacg gcggcaaaaa cgccttcctg
     6661 gtgttttttc tggtgtttgt cgtacagacc ggcatgctgg tgcagcacct ggctgcgcgc
     6721 aagtggccgc gcccgcccac ggcgccccag gtcagccaga gctttcactg gagcagctgg
     6781 ctgatcgggc tgatggtcgg ggggtttgtc ggcggcctga tgtcgggcct gacgcccttc
     6841 aagccggcgc aggccgttgc catggcgctg atcgcctgcg tggccggctc gctcggccac
     6901 ctggtgatga aagccctcaa acgcgaccgc ggcatcacca actggggcag cgaaggccgg
     6961 tcggtcaccg gcgccagtgg cctgctggac cgcgtcgacg ccctgtgttt tgccgcgcct
     7021 gtgtttttcc actcggttcg gtggtatttt ggcctatgag tctgcgtttc gagattttgt
     7081 atttatcagt cagggccgcg gaaccggctt tgccgggccg caagggccgc ggaaccggct
     7141 ttgccgggcc gcaggcgcag tgccccctcg gggggcagcg tagtacacga agtgacaagc
     7201 gtgggggccc atgagaattc ttggcattga tccgggcctg cagatcaccg gcttcggcgt
     7261 gatcgacgtg gagggccacg cgctgagcta tgtcgccagc ggcaccatca agaccaccca
     7321 cctggatcgc ggcaacctgc cagcgcggct caaggtgttg ttcgacggca tctgcgaaat
     7381 caccgcccgc tacacacccg acagctcggc ctgcgagatc gtgtttgtca acgtcaaccc
     7441 gcaggccacc ctgatgctgg gccaggcgcg cggcgcctgt gtcacggccc tggtcagctg
     7501 caacatcgag gtggccgaat acacaccact gcagatgaaa aaggcggtgg cgggttacgg
     7561 caaggccggc aagcccgagg tgcaggagat ggtgatgaga ttgctcaaac tgccgtcact
     7621 gccgggcaag gacgccgccg acgcgctggg cctggcgatc acccatgccc atgtgggagg
     7681 ctccgccgcc atcctggcgc gcgccgcgac gctgcagcgt aaatccaccg cgtccttcaa
     7741 ggacggccgg cattactgag gcgcctggct cagatcaccc ggtctgcaat caggatggca
     7801 aaaaagacga aggcggccac cagcgtccat ttgaggatgg cgagccccgc gcgcttgtag
     7861 cggacctggg aagtgatcgc atacagcgca aaggacgcgc cggccgcgaa cagcaaaagg
     7921 aaaatggcga cacgaaaaac cggcatgcat cctcctgcgt tcggcccggc agctaccagg
     7981 cccgcgccag ggtcgcaaaa ccctggggcg cttttttttc atcgtcgaag gtgacgactt
     8041 cccaggcatc ttcgtgcgac agcagctcgc gcagcagctt gttgttcagc gcatggccgg
     8101 aacggaaggc cgtgtaggcg gccagcagcg gcttgccgag caggtacagg tcgcccatcg
     8161 catcgaggat cttgtgtttg acgaactcgt cgttgtaacg caggccgtca gtgttgagca
     8221 ccttgtagtc atccatcacg atggcattgt ccaggccgcc accaagcgcc agcccgttgg
     8281 cgcgcatcat ctccacgtcc ttggtaaaac cgaaggtgcg cgcgcgcgcg atgtcacggc
     8341 tgtaggagcc gctgcccatg tcgaactcca cacgctggcc ggtggaatcc accgcagggt
     8401 ggtcgaagtc gatttcaaac ccaagcttgt agccgttgta gggcgacagg cgcgcccatt
     8461 tctcgctggc gccctgccct tcccgtacct ccaccggctt gagcacgcgg ataaaacgtt
     8521 tcggtgctgc ctgcagctca atgccggccg actgcagcaa aaacacaaac gaggaggccg
     8581 aaccgtcgag aatcggcacc tcttcggccg tgatgtccac atacaggttg tcgatgccca
     8641 ggccggcgca ggccgacatc aggtgctcga ccgtgtgcac cttggcactg ccgcttgaaa
     8701 tcgttgaagc caggcgcgtg tcggtcaccg cctgcgcgga aatgggaatg tccaccggtt
     8761 gcggcagatc cacgcgccta aacacaatgc cggtgtccgg cgccgccggc cgcaaagtca
     8821 gctcgacccg ctgaccgctg tgcaggccca cacccaccgc cttggtcagg gatttgaggg
     8881 ttctttgggc gagcatggcg ttattttagg ttggggccgg acaagacgca cctggtcatg
     8941 cgcaatgacg cacatggcct gtgtgattag cggtcccgcg atcgaatcac tgattgatcg
     9001 aatggaaagc gtagcgagca gcccgagctt gcgttgagga gcggagccgt acccaggtac
     9061 ggtgagcacc gcagatgcaa gatcggattg cgcagtagct ttacaccgat caatccgcct
     9121 gcttgcgcag gaaagccgga atctcgaagt cgtccatacc gcccgacgcc agcgcatcaa
     9181 ccttggccgc cgcctgggta cggttggtgc gccatacgct gggcacgctc atgccgccgt
     9241 aatccggctg cgcgtggccc atcgagcccg gcgcagagga tgcgccagcc accgaattga
     9301 gcgtcggcag gttgaagggc acgttgtcgg tgccagtgcg cagcacctgc agcggtggtg
     9361 catgacggcg tacgccctgg cggctgaggc cggtggccac cacggtcacg cggatgtcat
     9421 c
//
LOCUS       DAIDFG010000099         9421 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_99_length_9421_cov_16.958040, whole genome shotgun sequence.
ACCESSION   DAIDFG010000099 DAIDFG010000000
VERSION     DAIDFG010000099.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 9421)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9421)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9421
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99_length_9421_cov_16.958040"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1696 a         3151 c         2922 g         1652 t
ORIGIN      
        1 aaaccggccg cgccggccgc gacggcctgc cggccaacgc ctggatggcc tacggcctgc
       61 aggacgtggt gaaccagcgc cgcatgatag acaccagtga ggcggccagc gaggagttca
      121 agcaggtgat gcgcggcaag ctcgacgcgc tcctgagcct ggccgaagac acacgttgcc
      181 ggcgcgtgag cctgctgcat tatttcggtg aagacagcca gccttgcggc aactgcgaca
      241 actgcctggc cccgcccaaa gtctgggacg cgaccgaagc ggcgcgcaag atgctcagct
      301 gcatctaccg cgtgcagcag gccagcggca tcagcttcgg cgccggccac ctgatggaca
      361 tcctgcgcgg caaggcgacc gaaaaagtgc tgcagcacag ccacgacaag ctcagcacct
      421 ttggcatcgg tgccgacctg gccgaggtgc agtggcgcgg cgtgttgcgc cagctgatcg
      481 ccaagggcat ggtgcgggtc gaccccgacg ccttcaacac cctgcagctg ctgcccgacg
      541 cgcgccaggt gctcaagggc gaagtcagcg tgatgctgcg cgaacaggcg ttgggcggct
      601 cggccgaccg ccgcaagggc agcaaaaagg gcaccaagac ctcggccagg ggcctggccg
      661 aggcgtcgct caacgccggc gcgctggagc gcctggcccg gctcaaggcc tggcgcgccg
      721 aggtggcgcg cgagcacaac ctgccggcct tcgtcatctt ccacgacgcg accctgcgcg
      781 cgattgccga gcgggcgccg caaagcacgg cggacctgga gggcatcagc ggcatcgggg
      841 tgaaaaagct cgaagcctat ggcagcgagg tattgcgcgt ctgcaccact gaagcctgac
      901 cggccggcgc cgcagctgca gcgaaccgcc ggtcaagcca gcgaccccga cagtccatgt
      961 caacaccctc tgctgcaggg cttgctgcac aataccggcc tgtgggagcc aggaaaaact
     1021 tcactgccgc aggagtgctg accgcctttg tccgggcgtt gctggccttt gtctgcctgc
     1081 aatctgccct gctggccggc cctgtggggg caggcgagtt gctgacactg gaccagcagc
     1141 gcgccccgct caacacgctg caggcgctgg agttctggct cgatgtgaaa gcccaggcct
     1201 cggtggaaca agtcgaggcc ggtgcccaca ccctcgagtt tgcgccggtt cagcgcggcc
     1261 ggccctacga gcttgacaac gcggcgcttt ggctgcgttt taacgccgtg gtccagaatc
     1321 cgctgacaca ctggaaactg gagttgccca tgtccggtgt cgacaaggtc actctgtatt
     1381 accgcgacag tctgggcaga tgggtggttc aacaagccgg cgacagcctg cccatgagcg
     1441 catggccgca gccggggcgc tatccggtgt tctcgctgag ccatgaggtc gggcagctcg
     1501 tcacttatta ccttcgcgtc gagcatgccc gcatcccctt ctccgcgctg ccgcgcgtga
     1561 caagcgacgc gcaggtcacc acggcgcgtc agctggaaca cctgctgctg ggcgcctatt
     1621 ttggcctggc ggcgctggtc accgtattgg ccctgatcaa tgccacggcc tatcgggatt
     1681 gggggtttgc cacgtatgca ctgtatgtgg caacctttgc gggctcacag ggcgccttct
     1741 ccggcgtggc tggcctgtac ttgtggccgg agctgccgaa gctgaacaat atttccattg
     1801 tgctgctgcc attgacggcc gccgccgcag ccctctggtt tgtccggacc gtcaccaccc
     1861 ccaagcgctt ttcgcgggcg ctggactggt tcatcgtggc actgatggga ttgctgccgg
     1921 cggtcgcact catcgatgcg gcctttccga ccatcgaaag ttttgccctc ctcaatatcc
     1981 tggtcagcgc cggcatggtg gttgtgttga tggtggtcgg ggtgtcactg cttgaaggcg
     2041 atcgccatgc acgctggata gccctggggt ttttgcccat cattttggcc acccttttcc
     2101 ccttgttgcg caattacggc gtgctcgcgt ccagttttct cacccagaac gcgctgatgc
     2161 tgggctccgc cgtggaagcg cccatcctgt tttacggcct gttgcgccgc gtcacattgc
     2221 ggcgcgagtc cgaaacgcgt gcaacggcct tgcccacgac cgaccccctg accgggctgg
     2281 gtacggccag gctgctgctc ggcaggctgc gccaggccct gcgcactgcc gagcgttaca
     2341 agcaaccctg cgcgctgctg attgtcaacc tgagcaacct ccccagcctg cagcagcagc
     2401 acggccgcga gacgggcgac cgcgccatgg tgatggcggc cgcacgccta cgcgccgccg
     2461 cccagcccgc ggacacggtg gcgcgggtcg gcgacagcct gttcgccttg ctgatggaag
     2521 gccccatgag cagcgattcg gtgacccagg tggccaccaa gatcctggcc agcggcctgc
     2581 ggccctccag aaagctgccc gaggccgacc cgctgctttt ccatatcgcc atcggttatg
     2641 tggccgagtc ggcacggctg gggccggatc aggcccaggc ttgcctcaat cgcatgctgc
     2701 aggcggtcaa ggagatgaac gacgggtcgc gcaaggcgat ccgcctgatc catctctgat
     2761 ttaccttgcg gagaaatcgg ccgttagcgc gcgcccatgt tgcgtgagca gctatccatt
     2821 tcgcagcatt cgcaactcac tgaatgaacc ccatgccgcg agcgtcacgc acgtcgggct
     2881 tgatgcggtg ctcggcttcg agctgctcca ggaattcttc cacgctgaac tcgaccgaca
     2941 aaatatcggt ctggcgcttg acggcggcaa agtcacccgg gcagagctgg ttcaggcggg
     3001 ccagccgttc gcgcaaggca gcggtcagcg ccgcagtgtc accggccagc gcttccgtga
     3061 cgaacatctg ctcgcgctgg agcggcgtca gcggcatgaa cttgatcttg aaggtaaaac
     3121 gccgcagcgc cgcctgatcc aggctctcga gcaggttggt ggtgcagaca aaaatgccgc
     3181 tgaagcgctc catgccctgc agcatctcgt tgacctcggt gacctcgtag gtgcgttgcg
     3241 cgccgcgcct gtcctgcagg aagctgtcgg cttcgtcgag cagcagcacg gctttctcct
     3301 gctcggcctc ggcaaacatg gcggccatgt tctgctcggt ctcgcccacg tacttgctca
     3361 tcaggtcgct ggcctgcttg atgatcagcg ggcagtccag cgcccgggcc atgtgttcgg
     3421 ccagggccgt cttgccggtg cccggtgcgc cataaaaaca cagcgtcccg tgaccgcgcg
     3481 ccttgagggc ctcgacgatg cgcgggacgt cgaagcgcga ctccacatga agcatggaca
     3541 ggtcgtaggt ggtcacgcta tgccgtccgc cggcgcctgc cctgttgccc agcgcctggt
     3601 cggcgttttt gagctggcgc tcgatcaggg attccatgag cgccgtgtgg ccgccggaca
     3661 ggacggccgc catgccggcg gcatcggtgc tggcacccgg gctgcttgcc agcttggcaa
     3721 aacgcaccgc cgtgcggatc tgcgccggcg tcaggccctt gcggccggtc agcctggcca
     3781 ccaaggcgtc gctgacgggc acgccttcga gcgtcttgcg caccaggatc tcgcgtgcgc
     3841 ccggcggcgg cgacttgagt tccaggtggt aggcaaagcg gcgccggaag gcggggtcga
     3901 tctgctcgat gcggttggtc acccacaggg tgggcaccgt gttggactcc agaatctggt
     3961 taacccaggc cttgccgttg actgacgtgg ccgccggcgc ggacagctgc tcggagcgcg
     4021 ccatcagctg cgccgcttcg ctggagatcg gcgggaacac atcctcaacc tcgtcaaaca
     4081 gcagggcggc atgggcactg cccttgagga agacctgggc gatctgcagc gagcggtagc
     4141 ggtcgcgccc gcttagcgag ttgccgtccc ggtcggcata ttcaacttcg aacagatcca
     4201 ggccggccgc ctgcgcgacc accttggtca gctcggtctt gcccgtgccc ggcggcccgt
     4261 aaagcagcac gttgaccccc agctccttgc cggccacggc attgcgcagc aacgcgacga
     4321 gcacctggac atcctccagc gcgaaggaga aatcggccag ccccaggctg ctgcgctcca
     4381 ccggccgggt gaacaccgcc atcaactcat tctggtcgcg gtactcgcgc atcaacaccg
     4441 gtgggagttt ttcactgacc ttcatgaggt cggccaggtc ggtgatgttg tgctcggaaa
     4501 tcaggttttc gaccatgccg atgcgctcga gccgagagcc ggcgcgcagg gcctcggcca
     4561 cctcgttggc tttcacgccg gccacctcgg ccagcgcggc ataggcttca ggcgcattgc
     4621 tgaccttgaa ctccaccagc atggagcgca ggtcgcgctg gtaacgcgcc agcgtgccat
     4681 acagcagcaa ggcgcgttcg gcctggttca gctgcagcag attggccagg gcgtcgatgt
     4741 ttttctcgac cagcgtggac tgctttttga gcgcgtgggt cagccagtcg ccagtggccg
     4801 acagcaccga cagcaggtcc ttgggctggt ccttggcgta ttcgtcgagg tagaagaaca
     4861 gcgtgccttc ctcatacggt ccgcgccatg cgccatggcg actcatgaac gcggcatcac
     4921 ccagagcctc gtggccctgc cagaactcgt tgcccctgca gcggcggccc aggaattcac
     4981 gcaggcgctg caaggccggc aagggccaca ccaggtgccg gcccgacagc gacagaagac
     5041 tgttgaggtc gcgccgcaca ttgaacttgg caccctgccg ggccgccagc gtcagcacaa
     5101 aatgcgaaca catcaggtcc agcaccggcg cctctttcag gccgggagaa agcagcagcg
     5161 gaccatgcct gtcagcacta cccgaacgct tgctcattga gacacctccg gcctggaaga
     5221 aggccacagc agttacacat gcaccctgat gacgtatgcc taaccataac gcagcttcat
     5281 gcccgatgga agacgctaat tgaatacccc cgtgtgaatt gcgccacaaa accgcgccac
     5341 aatcgacgct tctcccggag gcctttccca tgcttgcttt gaccgatatt gaggctgcgg
     5401 cgcttcgcct gcaaggccag ttgcttgata cgccctgcgt ggcgtccaaa accctgtcgc
     5461 aaatcaccgg ggcggacgtt ttcctgaagt tcgagaacct gcaatacacc gcctccttca
     5521 aggagcgcgg cgcctgcaac aagctggtgc agctctcggg cgaagaaggc cgccgcggcg
     5581 tggtggcgat gagcgcgggc aaccatgccc agggcgtcgc ctaccatgcg cagcgcctcg
     5641 gcctgcgcgc tgtgatcgtg atgccgcgtt tcacgcccgg tgtcaagatc gagcgcacgc
     5701 gcggctttgg cgctgaagtc gtgctgcacg gcgacacgct ggaggagtcg cgcagccacg
     5761 ccctggccct ggccgaacgc gaaggcctgg tgttcatcca cccctatgac gacgaagcca
     5821 tcattgccgg ccagggcacg gtgggcctgg agatgctgca cgccgtcccc gacctcgata
     5881 cgctgatcgt ggccgtcggc ggcggcggcc tgatcgctgg catggcaacg gccgccaagg
     5941 cgatcaggcc cggtatcgaa gtcatcggcg tgcagacctc gcgcttcccg gcgatggtca
     6001 atgcgatcaa gggcacacac tacccccagg gcagcagcac gattgccgag ggcattgccg
     6061 ttggcacgcc cggcgtgatc acgcaagcca tcattgcgcg cctggtggac gacctcgttc
     6121 tggtggaaga aggcgacatc gagcaggcca tcgtcatgct gctcgaaatc gaaaaaaccc
     6181 tggtcgaagg ggcgggcgcg gccggtctcg cggccttgct gaaatacccg gagcgtttca
     6241 tggggaaaaa ggtcggcctg gtgctgtgcg gaggcaacat cgatccgctg ctgctggcag
     6301 ccatcatcga gcgcggcatg gtgcgcgccg gccggctggc gcgcatccgc gtgagcgcgc
     6361 gcgacgtgcc cggctcactg gccagaatca ccgccacggt cgccgatgcc ggtgccaaca
     6421 tcgacgaggt gcatcaccag cgggcattta ccatgctggc cgcgcagaac gtcgacattg
     6481 aactggtgat ccagacgcgc gggcgcgcgc atattgcgca agtgctggac gtgctgaaaa
     6541 ccgccggctt tgaagccatg gagcaacaat gatgagtatc gaaaccctgc aagcctccct
     6601 gcccgactat tttgcaccct gggtacaggc cctgggcctg caagtcgcct cgtttgacag
     6661 ccagggcgta accttgcgcc tcccgcagaa cccgcaactc tcgcgggtcg gtggcatgct
     6721 ttgcggccag gccatgatgg ccgccgccga cacggccatg gtgctggcgc tgatcaacca
     6781 ttttggcgcg ttcaaaccct gcaccacggt ccagatgaac agcagttttc tcaaacccct
     6841 gtcgaaccag gacgccctga tcgaagcgcg cgtgatacgc gccggcaaat cgctggcctt
     6901 tggggagatc gacattcgcg gcgccgacga cggcaaaagt gtctgccgcg ccacgacaac
     6961 ctacgctttg ctctaggggc gtcttctagc gcagatgttt gcccattttg gcggcaaaca
     7021 gctgggtgat gctcacgctg cgccggcccg gcacaagttc gaagccttcc cccgcgctca
     7081 gggtgcctgg cgtcaggacc gacaggtaaa agccgcaaaa gccgctctgc gccatcagcc
     7141 tggaagcccg cgcaaaaccc atggcggcat tgaatttgta gcagggctcg cgcggaatac
     7201 gcacctgaag ttcgcagtgt gggaacctca gcacgtcccc ggcccacacc tcgctttcca
     7261 gcagcccctg gagcgtgagg ttttcaccca gactgccaaa aggcaggctg tcgtccatct
     7321 ggtccaggcc ctgttcgcgg cgcgcagcct gccagaaggg gtagtgttct gccgggtagg
     7381 cataaacggc tttttcgagc ccgccatgca ccgaaagatc ggcctgctca tcaccgagca
     7441 ggcccagggg caacacaggc accgggccgg tcaaggcctg cttgcccatg gccgtgagca
     7501 cttggcgccc gcccatgctg acgcgccggg cggcggcaag ttgcacggat agcacctgga
     7561 gcgagagggc caagttcagt tccagccaat cacatcgaac cccttgtcgg ccaggcagcg
     7621 ctgcacatag gtgcggtagg tactgctggg ccggtcgttt cggaaagcgc cctgaaccgc
     7681 accggccgaa ccgccaacgg cggcgccgcc tgcaccggca cggagcaccc cttccgtgcc
     7741 ccttccggtg atcagggcac cgatagcggc ggccacgccg ccagtggccg cgccgacccc
     7801 ggcgccccgc gccaccgcat tggtgttctc ggcgggtgtc aatcctgctg ctgctgcccg
     7861 ggccatgcag gcatcggcct cggcacggcc ctgcgcgtca cctacgcgat ggagcgtggc
     7921 gttgggatac aggacgggcc gcgcagacgg gctggccgag ccggtgctgg cgcagcccgg
     7981 caagatgccc agcatcagcg caagaacaaa accgccgccc cggcgcgtca gatcacattg
     8041 catgcttcat aactccagag tagaactgca ataacgctgc ccgaatgcca ccggacgact
     8101 ggagaacggc tttccgtccg ctatgcggac cgcgtccatc cgggcggctt gcggccgtcc
     8161 gggccaaaaa ggtaaaatca tccaacttac tttcccccgt ttgcaggaac caccatgtca
     8221 agcccagccg atcgctccac caacgccgcc cacagcagcc ccacgccagc ccaggaacag
     8281 gacgccgtag aagccattgt gccgctgatg cccatcgtgc tgccggtcgc tggcggtgtc
     8341 ttgatgttcc tgctggcctt catcgccatt tacatggctt gaggcgattc gcggccaggc
     8401 gcgcattgtt aaacctgcgc acctcggagc acttggccaa taccgaagct cttgtcagac
     8461 ttcagttcac cgcactgaat caagaaaatt gatcccgatg tcgaggcttt cggcgtaagc
     8521 gcgacctaca gcgcctcgcc tgtatacatg cggttcaggt ctacgcctcc gcctccgcgg
     8581 caaacttacc caccctaaca cctgccaacc caacgccact gggttcggcc tgatggcttc
     8641 gaccaggcag caacggcgtc ccaggaccag cggccacacc gtcgagagcc acgtaacagc
     8701 gcccgcttta ggcgcgcatc gcgtatttca tccctccagg tcacgactgc ccgaaccctt
     8761 ccgtcctata tgcataaagt catgcaagtt ctgcatatct tttgcctgta acgtccatgt
     8821 aacccgtgcc gatgttctta aaatggcttt tccggcaggc cgtggaagcc tgaaaggcat
     8881 gccacaaact gctttttcga ctttgacccc acggcacatc cagactgtgg cccgcctgca
     8941 gaccaggact tgccgatcgc aggccgtgcc ttttgtccgg ctggtcgaag ccttgtgtga
     9001 tgcgcgcccg caagccttat ttcgacaaac gcctcgcggc cacagccctg ctgtctgcgg
     9061 gccggcgttt tccgaaatga cgctttttaa ctttggagct tttcgatgaa ccaccccgcg
     9121 atgcaagggc tgaacctgaa cacacccggt tatgtcaaga acgccaggct cattgcctgg
     9181 gtagccgaca tggctgcgct gtgcaaaccg gacaatgtgt actggtgcga cggcagcgac
     9241 gccgagtacc agcgcctgtg ccagcaactg gtcgacgccg gcaccttcaa aaagctcaac
     9301 gaagccaagc ggcccaacag cttcctggcc tgttccgacc cgagcgacgt cgcacgcgtg
     9361 gaagaccgca cctacatctg ctcggccgac aaggaaaacg ccggcccgac caacaactgg
     9421 a
//
LOCUS       DAIDFG010000100         9392 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_100_length_9392_cov_18.955018, whole genome shotgun sequence.
ACCESSION   DAIDFG010000100 DAIDFG010000000
VERSION     DAIDFG010000100.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 9392)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9392)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9392
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100_length_9392_cov_18.955018"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1622 a         3051 c         2960 g         1759 t
ORIGIN      
        1 cggggtccag gcccatgacc acacgtgggc cggccggcgc ggccagcagc aggtcgcgca
       61 ggttgtcggc aaacaccttg atggcgacct tctcggcttc ctcgcgcagg cggctgaaca
      121 aatcgcgctc gatggacagg ctgagcttga cgcgccaggt ccaggccaca catttgcgca
      181 acaggtcatc ggccgccctg cccttgtggc tccagcccag gtgcacggca atttttcctt
      241 cggcgatgct gggtttgccg ggctcgggaa tttcgggcaa cagcagcttg gcgtcgagga
      301 tttcgagacc gcggccgcgg aacaccgcca gcgccctgtg tgaaggcacg cggccgatcg
      361 gttcgtcgta gtcgaagtag tcgcggaact tgctgttctc cagattgttt tcatccttgc
      421 cggtgaccag cttggacgag agcaggcctt cgctccagag ccactggcgc agcgactgca
      481 gcagcgcggc gtcttcagcc cagcgctcgg acaggatgtc gcgcacgccg tcgagcacgg
      541 cctgcacggt ggtgaagtcg tcgccgtttt cgcttttttc ggctttcacg aaagccaccg
      601 cctcgtccgc cggcaccagc gccggatcgg caaacagctt gtcggccagt ggttcgatgc
      661 cggcctcgcg tgcgatctgg cccttggtgc ggcgtttctg cttgaagggc aggtacaggt
      721 cttcgagctc ctgcttggtc ggtgcagccg caatcgcggc ggccagttcg ggcgtcagct
      781 tgccctgctc ttcaatgctt ttgagcacgg actcgcgccg ctcctgcagc tcacgcaggt
      841 aaccgaggcg gaattcgagc tcgcgcagct ggatgtcgtc gagaccgccg gtgacttcct
      901 tgcggtaacg cgcgataaac ggcacggtgg cgccgccatc gagcagctcg acggcggtct
      961 tgacctggtg ttcctgaacc ttgatttccg ccgcgatctg gcgaatgatt ttttgcatgg
     1021 aggggagtga ttcgtcgtag gctgtgaatg ggaggacggg ctggcgccgg gcaagaggcc
     1081 ggcctcaaac cgcggcgtca agggtcggca ttgtcccaca gcgggaagcc ctggtcctga
     1141 cggctttccc aggttcctag acgtcggcag gccccaccgg tgcgcagagt tccggatcca
     1201 gcggcaaggt cagctgcacg cgggtcccgc tgccgaactt gctcgagatg gtcagttctc
     1261 ccccaatcag ccgtgcgcgc tggcgcatgt tgtccaggcc cttgccggca gcctctgaga
     1321 ccatgtcatg cgcgacgcct gcgccgttgt cccgcacctc aaactccatg cagcccatgt
     1381 ccgcggcgta gctgcaggac acgaccacct tggaggcctt tgcgtgacgc atcacattgg
     1441 acaggcactc ctgcgcaata cgaagaacct gccgcgcctc ctcgccgcga acggtttcca
     1501 gggcactgca tatctcgacg tcccagacca tgtggatgcc cagcttgtcg agggggcgtt
     1561 gcaccctgta gcgcagggag cccagcgctt cgacaatgtt ctggtcctcc gactcgatgc
     1621 cgtccaccgt cagtttgatg tccagcaaac attgttcaag cgccagaccc agggcttgct
     1681 gttcgggcgc gcggtggtcc agtgacgaca ggatgctgac aatctgggag gccacaccat
     1741 cgtgcaggtc ccgcgcaatg cggcgacgct cgtggcggac ggctgaggcc gtctcccctg
     1801 tttcctggct gctgccggcc ctggaggcgt cgcccgtgcc cagcagtctg aggcgcccgc
     1861 gccgggtcac caactgccag accaccagca ggagcagcac ataaaagtgc ctgaaaagga
     1921 tggcgctgtc ttctgtggca tctgccagcg tcacttgctg gatgcaaatg cccaggccaa
     1981 acatgccgcc ggccagcgcc aaccaacccg gggagctgcg cggcaggtag acgcaggccg
     2041 ccatggcgat ggtcagcagc atggccgaca gcaggttcat caccatccag ggggcgggca
     2101 cctctgcgcc gttggcgttg ccgttgtaat acagcgcaat cgctcccgcc aggacctgta
     2161 ccccgaggat ccagtaaatc ctcgcgctgg tcacatgcac cacagtgaac aggaaacagg
     2221 cggcactgag gagcaggaac tgaaacccga ggtgggcgac gtaagcgaca tgtgacgact
     2281 ctcccttgaa gcccagcccc atgctgacca gaaagtgggt cagccatagc agcagagcca
     2341 gccgcaaagc cccggcgagg gccgcgctgg aaaacccgcg ctgcaacgga cccgggtgag
     2401 gggattcaaa ccgtgccggc gatggacgca cacgctgggc gtgcgggggg accagaccat
     2461 cggagaatat gcgcgtggac atgatggtcc cctaaaacag gccgcgatgc gccgcataac
     2521 tgaccgcctg agcccggctg cgcacatgca gcttgcggta gatgttcttg atgtgggagt
     2581 tgatcgtctg gcaactgacg gcaagccgcg cgccaatttc gttgctggtg tagcccgcag
     2641 ccaccatttt cagcacttcc ttctcgcgat ccgacagcct cttgttgctc gttccgttgg
     2701 aagaatgccc ggggtcctgg ccattttgca gcttgtgcaa cagtcgccgc gcgaggtggg
     2761 gcgtgatgga ggcgccgcca ttggccacct gcagcacggc ttcgggaaag ttgccgaacc
     2821 aggagttctt cacgaagtag cctgtggcgc ccagtgtgaa ggcgcgcaag gcatgttctt
     2881 cgtcttccag agcggagatc accactgcct cggccatggg cctgacagtt ttcatgtggt
     2941 cgatcaggtc gaatccggtg ccgtcgccca gattaaggtc caccagcatc acgtcgaact
     3001 cgagctgcga aatcaggcgc cggccctctc gcatgttgct ggcctcccct accacatcgg
     3061 tgcgcaggtc acccagcaac tcctgcgaga tcaccttgcg gatgtggggg tcatcatcga
     3121 tcagcaggac ccgcaccggc tgcccgggct ggccgttgag gaagttgggc cacagcgagg
     3181 gggggccctc gtgtgctgcc tgcaggcctg gttgaagtcc gcgaatatcg cggggcgtgg
     3241 gctgcaacgc agccgtggtc tggatgatgt tttgcataac ttccctgtac tgttttttac
     3301 ctactcttca acggaaccgg tgcaagtgtg gagagtggag agtggagagt ggaaatctgt
     3361 tgcgttatga ctctcattaa acaaatcctg gatggcgccg gtggcaccac cccgtggcct
     3421 cactgaacac ccttgccctt gactgcttgc ccactcccaa aatcttgctg aaaaccttcg
     3481 ctgattcgtt acatcaggtc acctggttcg cgaggcagaa acatcgaaca tccgtgattt
     3541 ttttgcgcaa tggagcaatt aggtcgcaaa tgggtgaccc cattgatgtt tatttctgcg
     3601 tgtttccttg gatacagctt gttactgact atttcccttc gtcgaggcat ttcacaaatt
     3661 gatagcgctg tccccttgca gtgccgtcgt tgccacatcg attgctgaaa aggggagacg
     3721 gcttttctcc cccgattgaa ctactcacga ctggttattg gcgtaaccct gagtcatacc
     3781 caaactccac ctcgacaacg ccgaaagagc tgcaatttcc acctgaaaga gctgctctcc
     3841 accgcgaagt caatgtggct ggtcgccgga ctccctggcg accagaaggt cttaagtcac
     3901 tttttaagga aactgtcatg aaaaaacttg tactcctcgc cttgtccgct gttgctaccg
     3961 ctgcgatggc caccggcccg acacctgcca gcgaaatcac gatcaacggc aactccgccc
     4021 agatcgctgt gctatcaagc tccggcgtcg tcaacaccgc caacggtgaa gactccctgg
     4081 cccagcaaaa cctggccagc aatgccggtg atgtgacggt caatggcaac tcgctgcaga
     4141 tcgttggtgc acgcagcagc tatgtgggca atacctcgaa cgccaacacg gtggcccagc
     4201 agaacctgtc gtccaacatc ggtgaagtca ctgtcaacgg caactcgctg caggccacgc
     4261 tgatggacgc cagctatgtg ctcaacagcg cgaccggccg tgatgcgaca gcggtgcaga
     4321 acattgcctc gaacaatgcc tgcttcgcct gcaccacgac cagctctggc cacggccacg
     4381 gccactgaga gcgccgttcc agggcctctt tggtcgtcga atccgctgtc agcccaggct
     4441 gatgcaacgg tttcgcggcg cgaaggccct ggtggggcgt gccgggtcag gcggcgcctg
     4501 atccggcact tttccacttt ccttttcctt aacgtgtgga gattccgtca tgaaaacatc
     4561 atcgcaggta gtgggcgcag ccaccatggc cgccctgttt ctgatggggt ccgcgtgggc
     4621 acagaatgcg cccggcgagg atccggtcat tctgaccaaa cgcatctcgg tgcaaatggc
     4681 cccgatcggc ggcgcacgtc cgagctcgcc gctggagtcc atgaacaaca tgccggcatg
     4741 gatgaaggcc aaggtcgcac gttacgaggc caaggccaat tccgatcaga gcaacggcat
     4801 ctcgaccgag gaggacgcaa cccgctcggc cagttccgac ggattcaaga aaacctgcat
     4861 ccaggaagtt ggcagcacga cggtggccag tgctgcgccc ggtgcccggc aaaacggtct
     4921 ttccagcaac cagcagatcg tggtgctcaa gggtgatctc gtgaatatct gcaagtagcc
     4981 gtcgccactg ccgcgcggcc gaccaggcat gccttcaggc cgtcatttca aaattttcac
     5041 aatcgtcgcg gggagaacaa catgaaacca tccatccggc ttgcgcctgc caggtcccgc
     5101 ctcagctcgc tggccctcgt gctgggcctc gcgggcgccg tcctgctggc gggttgcgcg
     5161 acgccggccg atccgcgcga cgatgccaaa ttccagtcct atgccagcgc caccaaccgg
     5221 ccggtggttc ggcccgtgcg atcagtctcg agtttttccg attcgctgat gtgcatggac
     5281 tacctgttcc gtgaggcgga gatctccacc acgctgatca ccagcaagca gatccccgat
     5341 ttttcgaccc gggttccggt tgcgacgaaa gacatgatca tcacggcgct ctcgcagatg
     5401 tcccggctca gcaatgcctt ccgttatgtc gactacgaag tcgacatcgc gcgccaggac
     5461 acggtgcaga acctgaccac catcctgctc aacaacaacc agatccagct gcagcgccct
     5521 gccctctatt tctccggcgc gatcgcgttt gtcgaccaga acgtcatcaa caaccgcttc
     5581 gaggtgggca ccgctgcctc gcggctggac accggctaca gcaagaaccg caacgccacc
     5641 atcatcgggc tggagttgca catcggcgac ttccgttcgc gcaccttgat ccctggactc
     5701 gactccgcca atgaagtcat catcgcgtcc ggcgggcagg gcctggacct ggccggccgg
     5761 atcggcagct acggcgtgca gttcaatgtc ggtcgcgact atgcgcaggg ggccggtgcc
     5821 gccatccgta cgctggtgga gctcgccacc atcgaactgg tcggcaaatg ggcgcgggtg
     5881 ccttactggc aatgcctgac cctggaacag acccatcccg acttccagcg gcagttgcgc
     5941 gactggtacg accagggctc gccgctgctg cacaacaagc tggtacagcg ctcgctcatc
     6001 agccatggct atctggggcc aaacggcacg caactcgatg aaaacagccc ggagttccgg
     6061 acagcgctgg gacgcttcca ggccgatgcc ggcatggtgg tgtccggcag agtggatttt
     6121 ccgacctacg agcgtgccct gcgccacttc gtctcgctcg gcaaggacgg caaactcgtg
     6181 cagatcggct ggtccgcgac cagcgactcg ccgcaggcca acgcggctga ctaccagcgc
     6241 ccgggcggca cgaaccccta cggcccctcg gcctatggca gcgcagcgcc gtccagaaag
     6301 atcagcctgc agatcgaaaa cgtgctggtg ggacgctccg ctttcgaggt gggcgagcag
     6361 atcttcctgt cggcgagcct gtcacgcgcg tcctacctgt actgcttcct gcatgaagcc
     6421 ggcggcaagg tgatgcgcct gctgcccaat gccacgaatc cgaatgcctt gttgagcgcc
     6481 aaccaggcga tccgcattcc cgactggatg agccccacgc ccggcttcat catggatgcg
     6541 accagcgcgg gcaccgagcg cgcgggctgt tttgccaccg atgccgacgt cgccggaaaa
     6601 ctgccgcagt tcctgcaggc cccggcactg gtaccgttgc ggcaggccac cagcctcgag
     6661 gacatcaaca aggcgttcgc cagcagtctg ggaccggatg gctacacctt tgcgggtgtg
     6721 cagtggcagg tcgggcccag gcgcgccgcc gcgccacctg cagccgcgcg ttagtccgct
     6781 gcagaagccg gccatgttgc ggttcatcat ccgtgtcacc tgctgctgcc tgtggctggg
     6841 agcggtccaa gctgccggca gcatcgcgcc ggagccggct ttcgacggat caccgccgcc
     6901 tgcgccaccg ctgcgcgcgg cggatccgac gcccgaaacg ctcagcgcca ccgaattgcc
     6961 gccgcctgcg ctgcaggccg gggagcgacc ctcggcgctg gaggcggaag tcgcgcgcct
     7021 gcgctcacgg gcgctgtcgg cggagccgca accgggccgc gttgcgcggg agcaggcccg
     7081 cgcctcctgg ctgcttggcc tgctgtatct gcacggcatc ggggtgacag ccagtccggc
     7141 ggaggccgcc gtgtggtttg agcgcgcgcg tgcgctgggt gagccgctgg ccgccgctgg
     7201 cctggcctgg tgcgagatcg atggctgccg cacggcgccc aaccctgcag aggcgcagcg
     7261 ctggatcacg ctgctgcgcc gggtggacct gccgcgtgcc cagtacctgc agtggctggt
     7321 cgacgagcgg ctgtcgccac tgcacctggc aaccccgggc ttgcgcggcg aacccgcttc
     7381 cgcgcccccc gcgcctgacc ggcaactgct gctgagcgcg gcgcgcggtg gcaacgtgca
     7441 tgcccggata gaactcggct tcgaaagcat cactgccaac cggcccgcgg aagcgctgga
     7501 gcattttcgc gcggtcgcgt ccaggtcgca ggcggcttct gccaatgcaa ccttgctgtc
     7561 ggaacggctg cgcatcacac cgcccgcccg cagtccggcc tcggccgccg acgcctttgc
     7621 acgggcgcag cgcaaccacc gcggcgaagg ccagccggcc aatttcgtgg aggccatccg
     7681 cctttaccgc ctggcgcaaa gccagggcag cgagccggcc agaaggatgc tggagctgat
     7741 cttttcccgg cccggccccg acgggcaggt cgatgtcgcc tggatgcagc agctggccta
     7801 cctgaacctg gtcagcggcg tgcctgcgct ggacagcacc acggtccgcc gcgcgctgcg
     7861 ccgcgagccg acgccgctgt ttgacatgct gcccgcctac cttcgcaagt agccaggaag
     7921 ggaaagcaaa gaggcccgcc ggcccgatgg cgtgcaggcc ttgtgccgcc aaactccgct
     7981 accatttcgg gcatcagccc cggcggggcg cgtccacggg aatgtgatgg cagtcgaaca
     8041 aggcagtgag ctggtcaagg tggtggtgtt gctggctgcc ggcgtgctgg ccgtgccgct
     8101 gttcaaacgg ctgggtctgg gttcggtgct cggttatctg gcggccgggc tggccatcgg
     8161 ccccttcggc ctgggtttgt tcagcgaagc aggcacgatt ttgcacctgg ctgaactcgg
     8221 cgtcgtgatg ttcctgtttg tcatcgggct ggagatgcaa ccgacccggc tctggacgct
     8281 gcggcgccag atcttcggcc tgggcttggc gcaggtggtg gtttgcggcg ccctgctcac
     8341 ggcgctgggc atgggcgccg ggttgaaacc ggtggtggcg ttcatcgcgg ccatgggttt
     8401 tgtgctgtcg tccaccgcca tcgtgatgca gctgctcgaa gaacgcggcg acacctccac
     8461 cccgcgcggc cagcgcatgg tgtccatcct gctgctggaa gacctcgcca tcgtgccctt
     8521 gctggcgatc gtggcttttc tggcgccggc tgcgaacgac ggcgcgggtg tgtcgcgctg
     8581 ggtcagcatc ggcgtggcgc tggcggccat catcggcctg gtggcggtca gccgctggct
     8641 gctcaatcca ctgtttcgcg tactggcgcg ggccaaggca cgagaagtca tgacggccgc
     8701 agccctgctg gtggtgctgg gcgcggccct ggccatgcag tggggtggcc tgtcgatggc
     8761 gcttggcgcc tttgccgccg gcgtgatgct gtccgaatcc accttccgcc accagctgga
     8821 agccgacatc gagccctttc gcggcatttt gctgggcctg tttttcatgg gtgtgggcat
     8881 gtcgctggac ctcaagctgg tgctggctga ctggccgctg attcttggcg gtgtgctggc
     8941 ctacatggcg ttcaagggca tgggcattta cgccgtggcc cgcgtcacca aatcgaacca
     9001 tggcgacgcc ctgcaacgta ccgccatgat ggcccagggc ggcgagtttg cctttgtgtt
     9061 gtattcggcg gcggctgcgg caggcatcgt ggaggccagt gtcaacgccg tgctgacggc
     9121 cgccatcatc atctcgatgg cgctgacccc gctggcggtc atgtcgcttc gctggctcct
     9181 gcccgccaag ccggagcagg acatggacgg tgttgacgtt gcccaggacc tgcggggcag
     9241 cgtgctcatc atcggtttcg gccgtttcgg gcagattgcc agccagtcgc tgctggcgcg
     9301 cggcatcgac attgccatca tcgaaaacga caccgacatg atccgcgcgg cggcccagtt
     9361 cggcttcaag gtgtattacg gcgacggcac gc
//
LOCUS       DAIDFG010000101         9233 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_101_length_9233_cov_16.512966, whole genome shotgun sequence.
ACCESSION   DAIDFG010000101 DAIDFG010000000
VERSION     DAIDFG010000101.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 9233)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9233)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9233
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101_length_9233_cov_16.512966"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1726 a         3045 c         2939 g         1523 t
ORIGIN      
        1 gggcaggttg gccagcttgt agtgcgcgcg caggaaacgg ttgcacaggc cgtggaaggt
       61 gccaatccac atgccgcgca cattgaccgg cagcatggcc gacaggcgtg tcagcatctc
      121 cttggccgcc ttgttggtaa aggtcacggc cagaatgccg ccgggcgaca cctgcccggt
      181 ctgcagcagc caggcgatgc gggtggtcaa cacacgggtc ttgccggaac cggcgccagc
      241 caggatcagc gcgtgcgtgt tgggcagggt gacggccgcg agttgctccg gattaagatt
      301 ctgtaagagt ggggagcttg ggacgacttc tgagaacatc tccccattgt agaaagcctc
      361 cgatgcccca tccccttgcc gcccgagtaa ccgtgttatc ggcccttctg ctggccgccg
      421 gcctggccca gccggccctg gcccagtctt cctgcgccag cgatggccag ccgcgcccgg
      481 tggccctggt ggagcgtttc atcaatgccg actgcgccac ctgctggacc gacccggcca
      541 ccccgcccgc cgcccgcggc gccgtggcgc tggactgggt cttgcccggc ggccagggcg
      601 aggacgcccc gctgtctgcg gtggccacgc gtgatggact caagcgcctg ggggcgctgg
      661 gcacggcgct gccggcggcc agccagaccc tccgcacgcc ggtgcagggc cgcgcgggcg
      721 cgctgcgtgt ggcgcacggc ctgccggttt ccggctacat cggcgcgtcc atcgcgttca
      781 agggggcggc agcacgggcc accggccagc cctggacggg ctggctggcc ctggtggaaa
      841 cgctcccggc cggtaccgaa ggctcacccg tacctcgcca tcttgtacga aacctgcttc
      901 aagtccaatg ggatgggcgc aaccagctat caaaacagga gcaactgcga ttcatcgagt
      961 cgcgctccat ggatgtgccg gcgggcacga acaccgaccg gctgcgggtc atcggctggg
     1021 tgcaggacgc cagtgggcgc atcgtcgcgg cggcacaatc gcgctgcgcc ggaaaatagc
     1081 gccctccctc tctccatctg cctggccgta taacggctat acgccgccgc aactccgcgc
     1141 cccccgcaca agcgcctaga atcaatcacc gggcccaagc aattgcgccc ggttttagct
     1201 tcggcatgac aagccctgcg ggcacgtcag cctccgctga aactctcttg cgagagtttt
     1261 ttgttgccgt ccaggcagcg aaagccggtc cagtccaagt gccttctgtt cccaacagtt
     1321 tttaaaggct tggaatccat ggaaatcttc gactacgaca acatcctgct gctgccgcgc
     1381 aaatgccgcg tggaaagccg ctccgaatgc gacgccggcg ttacgctggg caaccggcgc
     1441 tttcgcatcc cggtggtgcc ggccaacatg aagacggtga tcgacgaatc gatcagtgtg
     1501 tggatggcgc aaaacggcta tttctacgtg atgcaccgct tcgacctgga caacctggct
     1561 tttgtcagaa gcatggcggc ccgggggctt tacgcatcga tctcgctggg tgtcaaacag
     1621 gccgactacg acacggtgga ccagctggtg gccgaaggcc tggcgcccga ctacatcacc
     1681 atcgacatcg cccatggcca cgccgacacg gtgcagcgca tggtggcttg tctgaaagaa
     1741 aaactgccca catcgtttgt gattgccggc aatgtggcca cgcccgaggc ggtgattgac
     1801 ctggagaact ggggcgcgga cgccaccaag gtcggcatcg gccccggcaa ggtctgcatc
     1861 accaagatga aaaccggctt tggcaccggc ggctggcagc tgtcggcgct caagtggtgc
     1921 gcgcgtgtgg cgaccaaacc catcattgcc gacggcggca tccgcgaaca cggcgacatc
     1981 gccaagtcca tccgtttcgg cgcgaccatg gtcatgatcg gctccatgct ggccggcctg
     2041 gaggaaagcc cgggcaaaac cgtggaggtc gatggccggc ttttcaagga gtattacggc
     2101 agcgcctcgg acttcaacaa gggcgagtac aaacacgtcg aaggcaaacg catcctcgag
     2161 ccggtcaagg gcagcctcaa ggacacgctg cgcgagatgg aggaagacgt gcagagcgcc
     2221 atcagttatt caggcggcac ccggttgatg gacatccgca aggtcaacta cgtcatcctg
     2281 ggcggcgaca acgccggcga gcacctgctg atgtaagact ttgtgcggca aggttgcggg
     2341 cagtacagtg gtcactcccc attgaaaacc agcatccgtg cccactgccg ccattgaaat
     2401 ccgtcgcaac tattccccgg acgaagaaac cgcgctgatt gccgccgtgc aaaccgcggt
     2461 gatcgaggcc ttgaaggtcc cgccctggac gatttcggtc cggctatttg tccacgaagc
     2521 gcggcgcttt gccggcccgg cagacaaggg cgagcgctac accctggtta ccctggactg
     2581 tttcaccggg cgttcgctgg caaccaaacg cgtgttgtac caggccatca cacacaggct
     2641 gggcctgtgc ggtattccag ccgaccatct caaggttttg ctgcgcgagg cgccgcgtga
     2701 gaactgggcc attcgcggcg gccaaccggc atctgatgtg gcgctcggtt acgacgtggc
     2761 gatctagccg cgccggttgg tatcaggctg tcgcggcaac cagttgcagc gccagctggt
     2821 gcaaccggtg gccgggctgc accagcgcct cgacgatgct gaaatggttg agccccagca
     2881 gggcttcgcg caccggcacc acgcgcggcc cccaggcctt ctcgatcagc gcgttgtggc
     2941 gcaggaattc ttcgctttca tcggcaccgg ccacggtata gagcacgccc tgtttgggcg
     3001 gcggcagcca ggccgggctg gcctgcgcga cctgcgcggg tgtcagctga agcgcgtctt
     3061 tgaggaaagg cgtgtgcatg acggagtcga gctcgtacag gcccgaaatc gacagcgcgt
     3121 ttttcaccag atcggccggc aggtccttcg catgcaccgg ccacaggcag gccagcagca
     3181 tggccgcgag gtggccgccc gccgaatgcc cgatcaccgt gatgcgtttc ggatcgccgc
     3241 cgaaacgcgc cacgttgcgg tacgtccagg ccagcgccct gaccatttgc agcgtgatct
     3301 cgggaatcgt caccgccggg cacaggtcgt aattgggaac caccacacag gcgcccgccg
     3361 ccgtgaaagc cggcgccaca aacgaatgct cggacttgtc gagtgagcgc cagtagccgc
     3421 cgtgcacaaa caccagcacc ggcgcgccgc ccggcgtggc ccgggctgca ggaaacacat
     3481 cgagtttctc gccggggctc gcgccgtaag ccaggtccag cgtacagggc tgactgctgc
     3541 gcgcctgcgc ggacgtctgg gcccagcggg cgaaatgcgc cgcgtgttcg gggaccaggg
     3601 cgcggttgtt gtacatgcgg tccagccagg cgggatcggg tcgggtcatg agggctcctg
     3661 aagaatcggc ataaagaact tgttccgccc gtcatcttgg cacagtcgga cgcgcccatc
     3721 aggccctcgg gtaaacccgc ttcagcttga ccgtcaatca tcgtattctg tatacagaat
     3781 taaaaccatg acaccccagc tcatcaaaat cgaatccagc cccgacctgg tggaccaggt
     3841 ctaccgaagc ctgctcgacg cgatcagtga gggctcgctg gcgccgggcg cccgcatcac
     3901 gcaggaggac attgccgagc agctggccgt gtcgcgccag ccggtgctgc aggccttgcg
     3961 gctactgaaa aaagatggct ttgtgctcga cgcccccggc cgcggcgtgc tggtcgcacc
     4021 gctggatgtg ggctggctga tgcaggtgta ccaggtgcgc agcgcgctcg atgcactggc
     4081 cgcccggctg gccgcgcggt cccgcttcca gatcgcgccg gcgctgatca gccaggggcg
     4141 cgccgccgca cgcagccacg atgtcaaggc catgatggcc gccgacgcgg aatttcacgg
     4201 cgccatttat gcatcctccg gcaaccccct gatcgcccag agcgcgcaac tgcactggca
     4261 ccacatccgc cgtgccatgg gcgcggtgct gcaggtgtcc accatgcgcg aatcgatctg
     4321 ggacgaacac gaggccatcg cccaagccat aggcgctggc gatgaggcca ccgccgaaaa
     4381 tctgatccgc cagcacggtg aagacgccag ccacaacctc tcgagcctgc tgtccagcgc
     4441 cctgcacccg acgcccccgt ccaccgccct gcccgcctga gaacaccgcc tgaccaccac
     4501 cccaaggaga cacccgtgaa cctgactgct gaacaactcg cccaattcga ccgcgacggt
     4561 tacctgtttt tccccggcct gttcacgccg gaagaaacac agaccctcaa cgacgccgtg
     4621 cccgagctct acagccggcg cgaggactac aacgtgcgcg aaaaaggcaa ggacgcggtg
     4681 cgtaccaact tcgcggccca catgtacagc gagccttttg ccaaactggg ccggcacccg
     4741 cgcatgatcg agccggtcga agacctgctc ggtgaaaagc tctacatgca ccagttcaag
     4801 atcaacggca agatggcttt tgaaggcgac gtctggcagt ggcaccagga ctacggcacc
     4861 tggctcaacg acgacatgat gccgaccgaa cgcgcgatga acgtggcgat ttttctcgac
     4921 gatgtgaccg aacacaacgg cccgctgatg ttcatccccg gcagccacaa aaaaggcgtg
     4981 gtcgatgcca aacacgacct gaccaccacc agctacccgc tgtggacggt ggacaacgac
     5041 ctgatccgcc agctggtgca gcgtgccggc ggcaagcaag gcggcattgt cagccccaag
     5101 ggcccggccg gctccatgat cctgttccac tcctgcctgg tccacgcttc gggcagcaat
     5161 ctgtcgccct tcaaccgggt cagtgtctac ctgagcctgt gtgcggtcag caaccacatc
     5221 cgccgccaca agcgcccgga atacatcgcg caccgcgact tcacacccat cgaatgcctg
     5281 ccggacgact gcctgctgaa ggactacccg atggacctgc cgtggaaaga cggcgtgccg
     5341 gccaccgcgc tgcagacctc gatggaagaa atcgtgccgg agcgcaaggc ggcctgaaat
     5401 cgacgacttc gggtgcttcc ggaaattcag aatttcggga tgaagtgcaa ggcgcccgga
     5461 gaacagcaac cggaatgtac ttcctgtaca tgaggattgc gagcaccgca caacgacgca
     5521 attcgcccga aaaatggatt tacggaagta cccaatgaat ctctatacaa aactccagca
     5581 acgcgcggcc gacaacaaac ccatccgcat cggcctcata ggcgccggca agttcggttc
     5641 catgtacctg gcgcagattc cgcgcacgcc cggcgtgcac ttggtcgcga ttgccgacct
     5701 gtcgccagcc gccgcccgca ccaacctcgc ccgtgtaggc tggaatccgg cccttaccca
     5761 ggctgcttct gcgcaggaag ctatcaaaac aggagcgacc tggatcaccg acgactggaa
     5821 tgcagtggcc acccatccgc agatcgacat catcgtcgag tgcaccggca acccggtcgc
     5881 cgcggtcgaa cactgcctgc aggcgtttgc gcacggcaaa catgtggtca acgtcacggt
     5941 ggaagccgac gccttttgcg gcccgctgct ggcgcgcaag gcggcgcaag ccggtgtcgt
     6001 gtattcgctg gcattcggcg accagccggc gctgatctgc gacctggtgg actgggcgcg
     6061 cacctgcggt tttccggtgg tggcggcagg ccgcggtcac aagtggctgc cgcatttttc
     6121 cgaatcgaca cccgagacgg tctggggcaa ctacggcctc acgccggagc aggccgagcg
     6181 cggcggactc aaccccaaga tgttcaacag ctttctcgac ggctccaaac cctcgatcga
     6241 atcgaccgct gtggccaacg ccaccggcct gacggtgccc agcaatggcc tgctgtatcc
     6301 gccggccagc gtcgaggaca ttcccttcgt cacccgaccg atcagcgaag gcggcgtgct
     6361 tgagcgcaag ggcatggtgg aagtgatctc ctcgctggaa gccaacggtc gcaagatccc
     6421 ctacgacatc cgcatgggcg tctgggtcac ggtcgaagcc gagaccgact acatcaagaa
     6481 ttgctttgaa gagtacaacg cgcacaccga cccgagcgga cgctacttca cgctctacaa
     6541 gcgttggcac ctgatcgggc tcgaagtcgg cgtgtcggtg gccagcgtcg cgctgcgcgg
     6601 cgaacccacc ggcgtcgcga cctgctggaa cgccgacgtg gtggccaccg ccaaacgcga
     6661 tctgaaaccc ggcgacatgc tggacggcga aggcggctac accgtctggg gcaagctgct
     6721 gccggccgac acctcggtga agatgggcgg cctgccgctg ggcctggcgc acaacgtcaa
     6781 ggtcgtgcgc gccgtcagga aaggccagag cctgagctgg gccgatgtgg ccatggacac
     6841 ctccaccccg gcgtacaagg tgcgcaacga gatggaaacc atgttctcgc cggccgtgaa
     6901 gaaagctgcc tgacagcctt ttttctgccg tgtgccgcat gatggtggcc accccacagc
     6961 ccggaacatt tcatgagccc cagcagcaag acagtcatcc cccgcatcgc ctttctgggc
     7021 accggcagca tgggcctgcc catggccagg cgcctgtgtg aggccggcca tgccctgcag
     7081 gtgtggaacc gcacgccggc caaggccgct tcactgacgg cattgggcgc gaccgttcat
     7141 gcacaggcca gcgcggcggt aacgggcgcc gacatcgtgg tcggcatgct ggaaaacggc
     7201 ctggtggtgg aagaggtgct gttcacccag ggggtcgctg cagcgatgaa gccgggcgcc
     7261 ctgttcatcg acatggcctc gatcaagccg cgcgaggcgc gcgaccatgc cgcacgcctg
     7321 ggcgcgctcg gtgtcgcgca cctcgatgcg ccggtgtccg gcggcacggg cggcgccgac
     7381 agcggcacgc tggtgatcat ggtgggcggc aaggcggcag attttgaacg tgccctgccg
     7441 gtgctccagg tcttcgggcg cgccacccac gtcggttccc atggtgcggg ccagctgacc
     7501 aagctggcca accagatgat tgtcggcatc acgataggcg ccgtggccga ggccctgctg
     7561 tttgccacca agggcggcgc cgacatggcc aaggtcaagg aagccatcac cggcggtttt
     7621 gccgacagcc gcatcctgca ggtccacggt cagcgcatgg tcgagcgcga cttcacctcc
     7681 cgcgcccgac tggcgatcca gctcaaggac atgcgtaatg cgctggccac cgcggaggaa
     7741 atcggcttcg aggcgccggt caccaggctg ttcgagcagc tgtacgccga aggcatagcc
     7801 cacggcctcg gcgagctgga ccattcgggc ctgtttgtcg agctggccag ccgcaacggc
     7861 atgagttgaa gcctcaagct gacgcccata aaaaaaggcc cgcaaagcgg gccttttttg
     7921 ttgcctgaac aacgatcagc gtgcagggcg tgccggacgc ttgtgggcgt cacgcggaac
     7981 aaacaccttg ccggcaggtt tggcaaacgc cggtttgcgg tctgcgaaat cgccgcgggg
     8041 ggctgatgca aaaccttccg gacggccgcc gaaactgcgc tcgccgccac gctcgtcgcg
     8101 ctgggcaaaa ccaccgttgt tgttgttgcc gaagccgcgg tcgtcacgac gcgagccgcg
     8161 gttgtcacgg tcgttgaagg caccaccagc cggtgcctgg aaaggcgcac ggtcattgcg
     8221 gttgttggag aaaccgccgc ggtcaccgcc acggtcatca cggttgccgc caaaaccacc
     8281 gcgatcggcg ccgaaggctg gcttcttgcc ggcgtaaccg ccgccagcgc cgccaccgaa
     8341 accgcccgaa ggcgcacggt cgttgccgaa acggccgccg ccaccgccgc cgaagctgcg
     8401 cggaccgcgg tcattgcggt caccaccgaa attgccgcgc gggcgatcgg aggtgggtgc
     8461 aaaacgctgc tgcggctcca ggccggcgat cacgctgggc ttgaggttct gctgggtgta
     8521 atgctcgatg tcctggatct tgcggcggtc gcggaactcg gcaatcgtga ttgcgtggcc
     8581 ttcgcggccc gcacggccgg tacggccaat gcggtgcaca taatcttcgg ctttcatcgg
     8641 caggccgtag ttgatcacgt gggtgatggt cggaacatcc agaccacggg cagcgacgtc
     8701 ggtcgcgacc aggatctgca cgcggccgtc acggaaagcc atcaggcggc ggttgcgcag
     8761 gccctggccc aaggcgccgt gcagcgccac ggcgctgaag ccctcttgca ccagatcgtt
     8821 ggccaggcca tcacactcga cctgcgtgca tgcaaacacg atggcctggt tgatggtggt
     8881 gtcgcgcagc cagtggtcca gcagcttgcg cttgtgggtc atgttgtcgg cccacagcag
     8941 cgattgcgtg atggacgcat gtttttcgtg cggggagtcg atctccacgc gctggggctg
     9001 gcgcatcacg cgcgtggcca gctgcatgat gcgcggcgca aaggtggcgc tgaacatcat
     9061 ggtctgcttg cgctcgatgg tgagctggtt gacttcggtc aggtcgtcgg caaagccgag
     9121 gtccagcatg cggtcggctt cgtcgaccac gaggaattgc acctgatcca gcttgatctg
     9181 catgctgcgc tgcaggtcca gcaggcggcc aggggtggcc accacgaggt cgg
//
LOCUS       DAIDFG010000102         9228 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_102_length_9228_cov_16.918892, whole genome shotgun sequence.
ACCESSION   DAIDFG010000102 DAIDFG010000000
VERSION     DAIDFG010000102.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 9228)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9228)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9228
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102_length_9228_cov_16.918892"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1748 a         2978 c         2840 g         1662 t
ORIGIN      
        1 acctgcgacg cgctcatcat cgccaccggc gcttccgcca aatacctggg cctggactcc
       61 gagaccgcct tcatgggccg gggcgtatcg ggttgcgcca cctgcgacgg ctttttttac
      121 cgcgaccagg aagtctgtgt ggtcggcggc ggcaatacgg cggtggaaga ggccttgtac
      181 ctgtccaaca ttgccagcaa ggtccatctg gtccaccggc gcgacaagtt caaggccgaa
      241 gccatcctga tcgacaagct ccacgaaaag gtggctgccg gcaagatcgt gctgaagctg
      301 caccagacac tcgatgaagt gttgggagat gccgccggcg tcaacggcgt gcggctcaag
      361 aacacggaaa ccggtgccac cgaagatctt cccgttcagg gctgcttcat tgccatcggc
      421 caccagccga acaccgacat ttttgccggc cagctggaca tgaaggaagg ctacatcgtc
      481 acccgcaccg gcttgcaggg ctttgccacc atgaccagcg tgccgggtgt ctttgccgcc
      541 ggcgacgtgc aggaccacgt ctatcgccag gcgatcacca gtgccggcac cggctgcatg
      601 gcggccctcg acgcccagcg ttttctggaa cagagttaga gcccccacac ttgtcgcttc
      661 gcgtactgcg ctgccccccg agggggcgct gcgcctgcgg accggcgaag ccggatccgc
      721 ggcccctgct ggtgaatacg gggagcctcg cccgtttcgt gacttcgacg acttcctgat
      781 gtcgctgagc caggaaaaac gcaagaaaat ccgccaggag cggcgcaagg tgctggaggc
      841 cggcgtgcgc tttcgatggt cgctgggcaa aggcatcagc actgccgact gggacttttt
      901 ctaccgctgt tacgagcgca cctactacga acacggcaac gcgccctacc tgagccgtga
      961 cttcttccag cgcatgcagg acaccatgcc ggagaactgg ctgctgtttg tggcagagcg
     1021 ggacggacgc ccaattgcta caagtttgat agctgtcagc gcaggcggaa caagggctgg
     1081 aggccaattt aactccataa catcgacccg ggtcgcctac ggccgctact ggggggcgct
     1141 ggaacgggtc gactgcctgc acttcgaggc ctgttattac cagcctctgc aatggtgcat
     1201 ggaacacggc taccagcgct tcgaaggcgg cgcccagggc gagcacaaaa tggcgcgcgc
     1261 cctgctgccg gtcaagacca ccagcgccca ctggctggcg cacccggcct ttgccgacgc
     1321 ggtggagcgc ttcctggaac gcgagggcga aggcatggcc ggctacatgg aagagctgga
     1381 gcggcgcaat ccgttccggc agatcgagcc cggcggcaat gccccctcgt gacgctacag
     1441 tattcatagc tgccagcgct tgccctcatt gggctggagg ctgatttggc gtataaggac
     1501 agttgattca agctttttct gacatcctgc tccacaccct tttttgacgg tttttttcat
     1561 gctcgatgcc accgacgccc ccggccccac aacgccggcc gacccgcccg cttcgctggg
     1621 ccgcgcccat gcgcggcgac tgcgcgagat ctaccgctcc gccggctggc cctgccagga
     1681 cctgctggaa atcgagttgc tggccgccgg cttgctgcag cgtgtcgccg gcccgtcagg
     1741 ccacgaaacc ctgcgcgtca ccgatgccgg cgtcacctgc ctggccgccg cactggtgcg
     1801 caaccgcgca gccctgtcgg cgcacgaggc gctggtcgaa cgcgtggcct gcgagatggc
     1861 gcgtgccggc cgcatcacct ggcgggcgtt gagcctgcgc gcgcagctgc cgccgccggc
     1921 cgacagcgcc gccgcccgct ggtgtttggt gcggcccgac gtgttttcga tccgcaatac
     1981 ctcggtggaa gcctatgtcg aacccgttgt ccacgagatc aaggtgcgac gctccgacct
     2041 gctgggcgac ctgcgccggc cagaaaaacg cggcgcctac ctcgatctgg gcggcgagtg
     2101 ctggtacgtc ctgggctgcg acgccaaagg caggccgatt gccaccgccg atgaagttcc
     2161 gccgaaatgc ggcgtgatgc aggtcgaagg cgagcggctg gtggtggccc gcgctgcgcc
     2221 gcggcggccc cggcaggcat tgcctttcgg cgtgtgggtc gcgctggcca aggccacgcc
     2281 cgtcgccggc ctgcgcgatg acgaacaggg cctgctgggc gatgccgaca tccaccctgg
     2341 ggagaaaaaa ccatcatgaa caaacaaccc gccggccatt accaggtcaa gctcgtcgag
     2401 tgccccagcc tcagccccga cgaacgcgct gcggcggagc tgcgttttcg catgtcgctt
     2461 gaattttccc tgggcggccc cgaccaggta ctcccttccc tgcaagccta cctgctggtg
     2521 caatcgctca acgaggactt gccgctgggc ggggatgcgt cggaagcgga ggaacaggtc
     2581 atcgcgctgt gggagaacgc cgaagaagac gcgatcctcg cggcatttcg ccccttgaac
     2641 cgggacatgg gtgatgcccg cttcgaaatc gttccgctat agtccaccgc cttcagcgcg
     2701 caagcagcag gaccaggctg gccagcgccg ccatgccgac gagcaccagc gcgcccgcca
     2761 gccattgcat gcggcgacgc tgcgtctcga tgcgcagaat cagctgcgtg ttctgctcgg
     2821 ccagcgcctt gatcagctcc gaggaagcga gcatctgttc ctgcagttct ttcgtcgccg
     2881 tttccagttc actgacatgc gcggtcaggg tggcgatggc ccgggcctcg ggcgacagcg
     2941 ccgcctggct ggcatcgtcc acctgcgcgc cggcggaagg cttcctgccc accgcgttcc
     3001 acaatttttt cgcgccagcc gcgaccttgg gtgcgttgct caccacctcg gcccacggga
     3061 cactttgcaa cacagtcaac cagcctatcg ccataaagcc acctttcggg aattctccac
     3121 acagaccgcc tgcaccaggc agcgtgcgaa catcaggtgt tcaccctaac atggcgggaa
     3181 aagccaccgg ggatccatgg caataatgcc catcgaagac ttcctgaggc ctgcccgacg
     3241 gggacaaaac ctgacaagcc cctacactga ggcatcaccg tttatgcaag gtctgttctc
     3301 gccaaagccg caaggccgcc gcccaggaaa ggagccatga tcaatttcac gccgcaacaa
     3361 tggcaaatgc tggtcgaggc cggtgcccgg gatccccagc cattgcacct tgccgacctg
     3421 agccagcctt ttgtgtacga caggatgatt ggcgtgattc actgcgcggc cggtcgccat
     3481 cgccttgcca tgtcactgct gctggccttc aggcacggct gcgacagcgg cattgaggtc
     3541 ggcgacaagc ttggactgga atttccggac gacacggccg accggtggct ggaagaaacg
     3601 ccgggagtgg cttttcgcag ttccgtgggt cgcaaggttc aggccggcaa gcgggccagc
     3661 ctgaccatca tcgaaaaacg ctggctgggc gaggtggagt acctgttcga agactagggt
     3721 tcaccctcgc acaggcgccg gctccagcgt aaagcggaaa accacacctt gtccgggcac
     3781 cggctccgcc cacaccgtgc cgccgtggcg gccaatgatc ctgcgcacac tggccaggac
     3841 aacaccagca cgtggggatt ccgtcgggct ccgcggagtc ccggactcgt ccgccgccgt
     3901 ctcgacagcg ccggacgtca tgccggagcc gttgtccctg acgaagtaaa ccacccgtcc
     3961 ttgcgccgat ggctccacgc caacgacgat ttcggcctcc ggattgcgtg cggtgtgttt
     4021 ccaggcgttg ccaatcaggt ttcccatgac ggtggcgatc aggcgcgcat cgccctggcc
     4081 gagcagcgag ttttcgacca cggccttgac ctgtcgctgc gggtcgcgct gccggcaggc
     4141 tgacaacgcc ttgtcggcaa tcgcggacag gtccagggtc tgggtattca ggggcgcccg
     4201 ggacacgtcg gcgagttcga gaagggcatc gatcatctca gccatgcggg ccacgccggc
     4261 ctggatgcgg tccaggtagt gcttctgctt gtcgtccatc gactcgccgc agccgcgtct
     4321 gagcaggccg gcaaagccct cgatcgccac cagcggagaa cgcaggtcat gcgccatcac
     4381 atgcgagaaa cgctcgagat cctcgttggc cgtctgcagt tctgccgtgc gatcacgcac
     4441 acggtgctcc agcgtcgcat tgagcctgag gacttcttct tccgcttgcc ttttcgctgt
     4501 gatgtcgcga ataaaggtac tgctcatctg ccggccccgg ctgtccgtgt aagacgatga
     4561 agaaagctcg gccaggaaag tggtcccgtc accgcgccgc aggaccaact ccccgcgggc
     4621 aaaaccggtc ctgctgcgct cctccaggaa gcctgccagc cgggggtcgc tggttacatc
     4681 gttgcgccca atccgccaca ggtcctggcg cgtgatgccg aacatggcgg aagctgccgg
     4741 gttggccgtg tgaaaggagc cgtctgcatt ggccatcagg taggcatccg gactgttctc
     4801 aaacagcagc ctgtactttt cctcgctttc caccagatca gcacgtgcac tttccagctg
     4861 ccggtaaggc ccctcgagga actcgatgaa gatggcccga aaaacgaaga ggtaggcctc
     4921 caccttgatg acatggccca ccatcgagta ctggtcgtgg aacgttgcat acagggtgaa
     4981 acacagttcc gacagggcca tcaggcaggc ggcggcaaaa agcggaatca gcggataggg
     5041 ctgcggcttg cgcatgcgca ggtaaaaacc cgcggccgcc gcgaggttga gaacaatgac
     5101 aaagtattcc accgtaatct tggtggtcgt cagcccggaa ccgggtacaa aaaaccaggt
     5161 tttgatggcc ggctcgacca gcccgtacac cgagaccccc agcgcaaaaa ccaggctcag
     5221 gcccagcagc cagtggcgtt cggtgtttcc aatacggcgg ttccagggct ggaacgcaat
     5281 cgccagcaag gcgcccgcca ccagcaggcg tgctgccagg aaaaacgaga ttgacttgcc
     5341 gtctttggcc gggatgaaga acgccggcat gccctcgtag gacagcaggt ggacaaaatc
     5401 aagaatggcc acgccgaaga agatggtgca aagcagcgct acattgctgc ggccctcccg
     5461 cgagaaagtg aaccagccgg cggaaaaaac cagcatcgac acaatgatgg acaccgtctc
     5521 gaacagggtg tgcaaattcg tgtgaaccgc ggcaggcagc tcaaaatgca gcgaggggac
     5581 cagcacaaac gcgagaagca gggcagcgtt gaccagccag acgccggaaa ccagcatcag
     5641 tggtcgccgg tataaagcgt ggggaatcat caagaaccag cctccatgcg cctgcgcctt
     5701 tttacaattt gtagcttcag tttaaggcac agtcaaccct cgtctgtccg cagaaacgag
     5761 gaaacactcg gtctgcctgg ggggacgcca tcccacccgt caaagctggc tgtcctggtt
     5821 gggcgaactg gccggcggcc gtgctcgcaa gacctccccg gggaacagca cttccacggt
     5881 gtaccccggc tcgcgtgacc acttctgttc cttgatcagc cactgcatga cgaactcccg
     5941 caccgtctgg cgcgccggct cgatcaccag ttgctggtag ccggctgata acgcgcgttt
     6001 ttccagctcg ggcgtgatcg actgcgccag cgccgccaga ttgtcctgct tgttcaggcg
     6061 cgcccagccg ttgtgggtgt acatctgcat ggccgaggtg tcgaaggcca cgggcaggct
     6121 gggcttgatg gcgggcgcct ggacaataca ggtcttgccg cggatcacca catgccactc
     6181 ccgggccagc gcgatgtgat aacggtaatg cacgggtgcc tggatgtgcg acaccgtcgt
     6241 gccaaggtgg atgccccaga actcccgagt gtccgagcgt gtgaaacgct cctgggcaat
     6301 caccgtggcc acttccagca aaccaccctc ggtacggatg acgaccggaa cctcgggcgc
     6361 gatcaccgtg gtctcgatgc gggtcgttgt tgcaggatag aagtgccagc cggcccaagc
     6421 catcagcgct gccaccacca cgatgagcaa ggcggcgccg ggaaggcgga tatgggttgg
     6481 catggtttct ggctttcttg cggacgctgc gaatgaattc aattatgggc cgcatccggc
     6541 gggccagaca gcccgggccc cggacgccga caacaacagg gggcaagctc aagcaagcac
     6601 caaggctgct gcgccggtga aaatccgcca aaccccgtct ctatttatcc tcctttgtca
     6661 cgcaggagac gactttaggg aagtctccct ttgtgaagcc gatctgccgc cccaatactg
     6721 gaacaatccg gaacaacaac gccaatatca atactcaccc catggcagta ccctcacccc
     6781 ggtctccagc acagaaaaag tcgcccgaaa aagcacgaca aagcccgcac gaaggcccag
     6841 cgcctagcgc ctggctgctg gcgctggaat ttcgcgcttt ctgggaattc ggcgcgttgc
     6901 tgcccgcgtg gcccgtgctg gcccgggcgc cccgcggaga cggccacagc gtggtggtgt
     6961 tccccggcct gtcggccagc gacggctcga ccatgccgct gcgccgctac ctcgattcgc
     7021 tgggctacca gaccagcggc tgggaacaag gcttcaactt cggcccgcgc gcgggcgtgc
     7081 tggaggcggc acgcgcccag gtgcggcgcg cgttcgagga cagcggccgc aaggtcagcc
     7141 tgatcggctg gagcctgggc ggcgtgtatg cgcgcgaact ggccaaggag ctgcagcata
     7201 tggtgcgtgg cgtgatcacc ctgggcacgc cgtttgccgg ctcgcaccgc tctaccaacg
     7261 cctggcgtat ctacgagctg gccagcgggc gcaaggtggc gcgggaaagc gagaactacg
     7321 acctgcccgc cgcaccaccg gtgcccacca ccagcattta ttcacgcagc gacggcatcg
     7381 tggcctggca aggcagtatt cagcaatcct cgccggacaa ccagcagaca gaaaacgtcg
     7441 aagtggtggc cagccacgtc ggcctgggcc tgaaccccag cgcctggtgg gccgtggccg
     7501 accggctggc acaacccgaa ggccgctggc aagcctttga gcgcaagggc acgttctggc
     7561 acgggctgat ttacccggac ccggcccgcg ccgcctgagc gtcgcccatc aaaaagaaaa
     7621 agagcgcctg cagcatgaac tgcaggcgct ctttttttga tagctacctg cgcttgactg
     7681 gcaagggctg gaggcaaaaa acgctttaaa tcaggtggct tcgggatgct ctttctggta
     7741 gttggcaata ccgtccatga tttccttgcg cgccgcgtcc ggaccttccc agccctggat
     7801 cttgacccac ttgccgtctt cgaggtcctt gtagtgctca aagaaatggg caatggtttt
     7861 caggcgcagc gggttcatgt cctcgggctt ttgccactgg gtgtacagcg agcatttctt
     7921 gtccaccggc acggccaaca ccttgccgtc ttcgccggct tcgtcggtca tcttcaggat
     7981 gccgatggcg cggcaggtta ccaccacacc agggatcagc ggcaccggcg tgatcaccag
     8041 cacatcgacc ggatcgccgt caccgctgag cgtcttgggc acataaccgt agttggtcgg
     8101 gtaatgcatg gacgtgctca tgaagcggtc cacaaaaatg gcgccggact ccttgtccac
     8161 ctcgtacttg accgggtcgg cattcatcgg gatttcgatg atcacgttga aagcgtcggg
     8221 ggcgttttta ccgggggtaa ctttatcgag ggacatggtc tctttgtgtt gaagtaatga
     8281 aaaggtggat ggctcagtca gtatttaccc tcattttact gattcggacc tgacgcttcg
     8341 cgaacttgtc acgatttggc gttaaatttc aaaggttggc caatcgtctt gtctctacgt
     8401 aggacacgag caacgaggaa gcaacgcagc gggggcaccg ctagcgtggc gcctcctcca
     8461 agcgaaaaac aacgtaggag atctctctat gacaggcaac ccagcgctta ttcttgcgct
     8521 cgtctgcgga ctggccgccg tggcttacgg cttctgggcc cgcagctgga ttctttccca
     8581 ggacgcaggc aacgcgcgca tgcaggaaat tgcagccgcg attcaaaccg gcgccgccgc
     8641 ctacctggcg cgtcaataca aaaccattgc catcgtcggc gtcgtgctgg cgattttgat
     8701 cggcatcttc ctcgatgtgc cgagtgccat cggttttgtc gtcggcgccg tgctttcggg
     8761 cgcttgcggc ttcatcggca tgaacgtctc ggtgcgtgcc aacgtgcgca ccgcacaggc
     8821 ggccacccgc ggcattggcc ccgccctcga tgtcgccttc cgtggcggcg cgatcaccgg
     8881 catgctggtg gtcggcctgg gcctgctggg cgtgacggcc ttttactggt tcctggtggg
     8941 caacggcaac tacacgccca atgccaacct ggccaacctg ctcaatccgc tgatcggttt
     9001 tgccttcggc tcctcgctga tctcgatttt tgcgcgcctg ggcggcggca ttttcaccaa
     9061 gggcgctgac gtcggcgccg acctggtggg caaggtcgaa gccggcattc cggaagacga
     9121 cccgcgcaac ccggcggtga tcgccgacaa cgtgggcgac aacgtgggcg actgcgccgg
     9181 catggctgcc gacctgtttg aaacctacgc ggtgacgctg atcgccac
//
LOCUS       DAIDFG010000103         9160 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_103_length_9160_cov_19.215157, whole genome shotgun sequence.
ACCESSION   DAIDFG010000103 DAIDFG010000000
VERSION     DAIDFG010000103.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 9160)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9160)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9160
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103_length_9160_cov_19.215157"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1666 a         3014 c         2927 g         1553 t
ORIGIN      
        1 atagaaaatg gcaaagccgt acatggcgtt ttccggcgtg ccggccttga tctgtgcgcc
       61 tatgaccacc ggggcgatga aggcgccgta ggcggcaatg gccgaggtcc agcccagcac
      121 cgggccggcc tgctggcggt cgaagatgac gccaatcgtg cggaaggtgg agccgttgcc
      181 gatgccgctg gcggcaaaca gcacgacgaa cagcaccatg aagatcagga agtactgctc
      241 gggcgcggcc gacttgtagg ccagcagcat gacgtaacct acggccacgg aggccaccac
      301 catgatggcg gagatgatct gcgtcacgat ggagccgccg accttgtccg agacccagcc
      361 gcccacgggc cggatcagcg cgcccacaaa cgggcctatc caggcaaagg tcagcgccga
      421 cggtgcgttc gggtttttca gtgtgtgctg catgacgccg gcggcgtccg gcacgtggct
      481 gatgccgaag atcaccgtga tcgccagcgg cagggccatc gaaaaaccga tgaaggaacc
      541 gaaggtcacg atgtacagca tggtcagcga ccaggtgtgc ttgttgctga aaatggagaa
      601 ctgtttggca atggcttcct tcatcggacc gaaggccgtc accttcaaca ccatcagggt
      661 gctcagcacg atcagtggca tggcgatcca catgttgagc aagcccaacc cggtaggttt
      721 gggcaggtag aggtacagcc cgacaccggc cggcacaaag gacagggtgt acagccagat
      781 gatcttgagg aaggccggga tcgtgccgcc gatggcgggc gagacggtgg tcaggttgtt
      841 catgccgaag aagcagatga ccgacagcgg tatcagagac agcagccagg caaaaccggc
      901 gttctggatc caggtcggcg ttccggccgt gatttttccg agaatccagc cgctgtcctt
      961 gaccagcgtc atcgattcgc ctccgaaact gccgaagatg ccgacggtca tcaccagggg
     1021 aatgacgacc tgcatggcgg tgacgccgaa gttgcccagg cccgcattaa gcccaagcgc
     1081 cgtgccctgc agccgcttgg gaaagaaggt gctgatgttg gacatcgaac tggcgaagtt
     1141 gccgccaccc acgcccgacc acagggccat cagctggaag gaccacagcg gccattcggg
     1201 gtgctgcagc acgatgccgg tgccgatggc cggcgccagc agcatcgaag tcgtcaggaa
     1261 gatggtgttg cgtccgccgg ccagccggat caggaaggag gccggaatgc gcatggtggc
     1321 gccggccagg cctgcaatcg ccgtcagcgt gaagagttcg gcctgggtga agggaaagcc
     1381 caggttgagc atctgcaccg tgatgatgcc ccacatgccc cagacggcaa agccgcacag
     1441 caggctgggc acggagatcc agaggttgcg gtaggcaata cgtttgccgg tgctttccca
     1501 gaactgctcg tcctcgggac gccagtcggt aatgtcaacg ccggaactgg ttttggtatt
     1561 catggtgttt cctcgaaaag ggtctgtttc aggcctgggc gccggacgcg ttcctgccca
     1621 tgacttcagt gcggcgcacc tcggtccagt acatccagat cagcgagacc cagacgacgc
     1681 cgtacatgaa catgaaagcg ctggaacgga tgcccgtcag gtccatcagg ccgccgaaga
     1741 tgatgggcag cacgaagccg cccaggccgc cggccagccc gacaatgccg ctgatcgcgc
     1801 cgatgttggc gccgtagtcg tcactgatgt acttgaaaac gctggccttg ccgaaggccc
     1861 aggcgatgcc caggatgaac atcagcgcgg tgaaggtgta gacgttcagg ccgaggtgaa
     1921 aggtcgccgg gccattgacc gtgagaatgg tgaagtcggt ctgcgggtag gacagcagga
     1981 acaggcacac ccagctcacc cacatcaccc accaggtcac gctgtgcgcg ccgtacttgt
     2041 cggacaggat gccgccaaag gcccgcaaca cgccgcccgg cagcgaaaaa cacgccgcca
     2101 acagcgctgc cacgcggatg tcgaggccga attcgcccac gtagtactga accatccaga
     2161 gggacagggc cacataacca ccgaacacga tgctgtagta ctggcagtac ttgagaactt
     2221 tcgggtcctt caaggccttg agctggtcac tgaacttgac gtggctgggc accaggtgcg
     2281 aagggtcgct gtagctgaac agccagaaca gcaccagcgc agccagcatg atgaccgcat
     2341 acacctgcgg caccatggcc cagccaaagg ccaccagcaa caccggcgcc acgaacttgt
     2401 tgacggcggc acccgagttt ccggcgccgt acacacccat cgccatgccc tggcggtttt
     2461 tcgggaacca gcgcgccaca taaggcgtgc ccaccgagaa ggagccgccg gccaggccga
     2521 caaacaggcc gatcaccaga aaatgccagt aggccgtggc gtaactcatg agccagatcg
     2581 cgggcacggt gatggccatc aggatcgcca tgacgatgcg cccgccgtag cggtcggtcc
     2641 agatgcccag cggcacgcgg atcagcgagc ccgtcagcac cggcgtggcc gcgagcaggc
     2701 cgaactcggt ggcattgagg ttgagcatct tcttgatcgg gatgccgatc acgccgaaca
     2761 tcatccagac catgaagcag acggtgaatg ccagcgtgct gacaatcagc accgaccatg
     2821 cttttcttga attgcccagt tcagaggcca tgttgtttct ccagacacat ggcatgactg
     2881 tcggcctttt gcaccccctt gaacattctc ctttggaacg gggcggcgtc agttaaaacc
     2941 gatggcggcc cgcgcgacca tcgttctgaa gaactactac cgggtcaagc tgccccggac
     3001 ttgatctttg tcaaccaagc tcccggccgg ccgcgtatac ggctgcctgg acccgcgaac
     3061 tgagctgcag cttgcgcagg atgtgctgga cgtgaatctt caccgtcgtc tccgcgatat
     3121 cgagcttgcg ggcaatcacc ttgttgctgt cgccgcgtgc gatcagcgcc agcacctcgc
     3181 gctcgcgcgc cgacagcagc gcgatggccg gctcatcctg caggcccggc accggggcgt
     3241 ccccgttgcg gccgccattg accgcagcga cctcgccatt ggaggtcggc ggggccttgg
     3301 accggaaagc ggccaccagc ttggtcatca tttccgggct gaccaccgac tccccgtcca
     3361 gcaccttgat gatggattcc gacaggcgat cgagttccac ggttttcagc aggtagccgt
     3421 cggcgccggc ctgcagcgct gcggacaggt cgtcctcgtt ctcgctcacc gtcaacatca
     3481 ggatgcgggt gccgggtgcc gcgtccttga gcgtcgcgat gccgtccacg cccagaacgc
     3541 ccggcaggtg gttgtccagc aggatcaggt ccggcttgct gcgttcgacg cagcgcagtg
     3601 cctcaccgat gtcgccggcc tcgcccgcca catggaaacg cccgtcctgc gagagcagcg
     3661 cgacgagacc gcggcgaaac agggtgtggt catcgaccac cagcagggca atgggcatcg
     3721 tcatgtcctc agtcgccgtg cgcgcccgcg gtgagggccg cgacggccgg ggtcttggcg
     3781 gcgacgccgc tgccgacctc cggatggggt ggcagtgtca gtgtcacggt ggttccctgg
     3841 ccagtccggg aaacaatatc gaccacagcg cctatgcgcc cggcacgttc ccgcatgatg
     3901 ttcaggccga catgggactg cccggggtgc ttgccggtgt cgaagccggt gccgttgtcg
     3961 cgcaccacaa aacgccagtg ctcacccttg atcacttcaa gtcccacttg cgtagcgccc
     4021 gcatgtttgc ggatattgga cagagcctcc tggagcacat gcagcacctg cacctgcacg
     4081 tccgaaggca gcggcagccc ctgcccctgc acctgcaact gggcaggaag gcccgtctgg
     4141 ctctggaact tctgcagggt ttcctgcagg gcctgctcaa tgtcgtcggt attggtgcgc
     4201 gtgcggaaat gcaccagcag ctcccggaca tcgctgatgc tttcgcgcag cccgacgtcc
     4261 agttcatcga gcgcaccctg cacctggggc gcctgcccct tgtgcaccgc gcctctgagc
     4321 aattgcacct gaattttcag aaaagccagc gactgggcaa tcgagtcatg caggttgcgc
     4381 gccagcagcg cgcgctcctc cgccaccgcc gcctcccgat ccagggcatc ggcgcgcaac
     4441 ccttccagcg aactggccag gtggctggcc agcgcatcca gcagttcgat ctcgtcggca
     4501 ctcagcagga ccggcgtgcg aaagaacagg ttgaactcgc cgagcaggcg ctgttgcagg
     4561 cgaatcggca ccgtgaccac actttcatag cccgccttgg cgcagtggcg caccggcgct
     4621 tcgtcgtggc tgaggatggg aatcacgcgg gtacgtgcgt ccggccgcag gttgccgcag
     4681 gcgcaggcgc cggccagcag gctgcgctct tcctccacca gctcctgcgg gaagcagtcg
     4741 gaagccagca tcaggtagcg ctggttggca tcatcggacc agcggacagc ggcagcgtcg
     4801 gccttcatca cggcgcgcac gcgctgggca aagccgcgtg ccagctcgtc aatgctgccg
     4861 gcctgggcca gaaaggcgct gaccttgtac agcgcctcca gccgcgcgcg ttgcgcctcg
     4921 atgcgccgcg tcttggcctc gaccttggcc tccagccctt catagagcga ctggagggtc
     4981 gccgtcatgt ggttgaagcc ggccgccacc tggccgaact cgtccacggt gtcgacctcg
     5041 atgcgcgttc tgaaatcccc ggactccagc ttgcgcaagc cctcccgcag ctgtgccagg
     5101 ggattgatca cgtacatgta gccggtgaaa agcatgatga ccgccccgcc gatggccagc
     5161 accatcatca ggaactggaa caggttcagg atggcggtca gccgggacag gtgctgctcg
     5221 atcaccagca ccagccggtc gctggcgtcc acaaacgcgc cccccgcctt cagcaggtcc
     5281 tgcgccgccg gcgccggctc ggccagccac aacggccgct gggtgcgcca cagggactcg
     5341 acatcgttga aacgcgcccg cacctggtcg tcccagggca cgaacagcgg cctggaaggg
     5401 tcgccctcgc gcagcagggc caggctctca tcgaaccggg ccaccagcgc ctgcagttcg
     5461 gtgcgttcca cgccggcctg cacggcgctg gccagacgcc aggtctgcat gcgcatgcgc
     5521 ccggcttcgt tgacggcggc ggcgccgcct tcgagctgcc aggtcaccca cagggtcagc
     5581 ccgatggaga ccagcgccac caccagcagg ctggccccga tgcgcatgag ttttttggag
     5641 agcgaggcgg tgtgctgcat gcgctagggc ctgttcacag gcaggcgcgc gccgcgggcg
     5701 gcagcggccg gcgccgcaca ggcgctgcac accatggcgg ccaggcgctg cagcgcctgc
     5761 cagccctcgg ccggccagtc gggctgcttc aggcctttga cgatgccgtc gaccttgtgg
     5821 gcggcatgca gcaaattggc cagcgtggtg tcgcccatgt ggggcaacac ccgctcgaac
     5881 agtttttcct tgacacccca gacgcggttt tcgcgcaggg ccatcggcat gggccggccc
     5941 gcgcccaggg cgtccttgac ccgcttcatg ctgcgaatgt cctcgctcag ggcccagtgc
     6001 accagcacct ccgactcacc ctcggactgc aaaccgtcga gcatgcgttg cacgcgcagg
     6061 gcctggccac cgagcaccgc ttcggacagc ttgaacacgt cgtaacgcgc gacattgagc
     6121 acggcgtttt cgacctggtc gaaactcagt tctgccgacg cctccccgcc gggggggtac
     6181 agcaggccca gcttctggat ttcctggtgc gcagccagca ggttgccttc gacacggtcg
     6241 gcaaaaaact gcagtgtgcg ctgcccctcc tcgccctgcg ccacgcgctg gccctgctgc
     6301 tgcaggcgct gcgcgatcca ctgcggcagg ttacggcggt caatcgggtc gaatttgacc
     6361 gtggtgccaa aactttccag cgcgccaaac cacgcgccct tggtcgtcat gctgtccagg
     6421 cgcggcagca gcaccagggt cagcgtggtg tcatcgccct gcgcgcgttc ggcaatctgc
     6481 tgcagcgcca ccgagccgtc cttgccgggc ttgccactgg gaatgcggat ctccacgatc
     6541 tgcttgtcgg caaacaggct cagggagccg ccgctggcca gcacctcgct ccagtcgaaa
     6601 tgggcaccgg ccacggtgtg caccgtgcgt tcggtgtaac cccgctcccg cgcggcggcg
     6661 cgcagcgcgt cggcgaactc ctgcaccagc agcggctcgt cgccgtgcag ggtgtacagg
     6721 ctcttgagcc cgcgcttgag gtggtcggaa agttgggcgg gcgccaggtt catggtggcc
     6781 caagtttagc ggttgggggt ttggcagcaa gccctcctga cctggcaggc gtggtctgct
     6841 ggcggcatac tccttcccat gacacaacgc attcgcctga ttggcgcacc caccgacatc
     6901 ggcgccggca cccgcggcgc gtcaatgggg cccgaggcgc tgcgcgtggc tggcctgcag
     6961 gccaccctgg aaggccgcgg cctgcaggtg aaggacggcg gcaacctgag cggcccgccc
     7021 aacccgtggc aggcgcccgt ccagggttac cgccacctgg ccgaggtggc cgcctggaac
     7081 cgctcggtgc acgaggccgt gttttcggcc ctcagcgaag gcgacctgcc cctcctgctg
     7141 ggcggcgacc actgcctggc gatcggctcc ataagcgccg tggcgcgccg ctgccgccag
     7201 accggccaga ccttgcgtgt gttgtggctg gacgcgcatg ccgacttcaa taccgaccgc
     7261 ctgaccccca ccggcaacac ccacggcatg ccggtggccg tgttgtgcgg gcacggcccg
     7321 gccgagctga ccggcatagg cggtgtggtt ccggcgatcc aggcttcggc cgtgcggcag
     7381 atcggcattc gcagcgtcga tgagggcgaa aagcacttca tccggcagtc gggcctggaa
     7441 gtgttcgaca tgcgttacat cgacgagcag ggcatgcggc agaccatggc agcggcgctg
     7501 gccggcgtgg atgcacagac tcacctgcat gtgagcttcg acgtggattt cctcgatgcg
     7561 atgattgcgc ccggtgtggg cacggccgtc aagggcggcc cgacttaccg cgaagcccag
     7621 ctgtgcatgg aaatgatcgc cgacaccggc cgcctggcct cgctggacgt gatggaactc
     7681 aacccggcgc tggatgtgcg caacggcact gccgaagtgg tgctggaact ggttgaaagc
     7741 ctgttcggca aaagcacgct gatgcgctga gcttcaagag accggccaca tcggcgctac
     7801 caaattaata gctgcttgca tccgtcctta ttgagctaat gcccgatttc atcatctatt
     7861 ccgccctgca gcgcagggcc gccgcgtcag atctccttga cggctgccag acggcgcagg
     7921 atctgctgga cgatgtcgct ctgcatgtcg cggtagagga gcgcctcctc ggtttccttg
     7981 gccagcaccg cggactcgtt gaaactgatg tcgcgctgca gcacgatttc ggcgggcgga
     8041 atcagcaact tgcccttgag cgtgcgcagc tggaacctga aacgcgtgcg cagctggaac
     8101 tcacgcacct ggcccgaggc gttgatgccc accacggttt tttcacgctg gtcgagcaac
     8161 acgtcgagaa tcacatccgc ctggccgatc aggcgcgcgt cgcggatcac cttcaccgtg
     8221 ccgccggctt ccaggttgcg gttgagctcg gtacccagcg tcgaggtttc ggccgcacca
     8281 ctgtaaatgg tcttgaaggc gtagttgggc gccttgcgca aggcaaatcc gcaggcactc
     8341 aggccccaag ccgccgcagc ggacaacagg aaaacgcgac gttgcatggt ggcttccttc
     8401 agacgacgat gttgaccaag cggcccggca caacgatgac ttttttggcc ggggcgccgg
     8461 cggcctgctt gacaaacgca tcggacgcca gcgccgccgc ctcgatcgcg gccttgtcag
     8521 cgccggcggg cacggtgacc gacccgcgca gcttgccgtt gatctgcagc acgagttcga
     8581 tctcgtcctg caccagggca gattcatcga cctccggcca ggacgcatcg agcaggccgc
     8641 catggtcttt gacaaaaccg agatcctccc acagggcgtg cgccacatgc ggcgtcgcgg
     8701 ggtagaggca gcgcagcagg atgcccatgc cttcgcgcag cgcaaacgcc gcgccggtcg
     8761 aggtctggcc cttgaagtct tccagcgcat tgagcagctt catgctgccc gacaccacgg
     8821 tgttgtactg catgcgctgg tagtcgtagt cgatctgctt gagcaccgtg tggatctcgc
     8881 ggcgcagcgc ctgcgtttcc ttatcaaatg tggttccagc cctttgcggg cgggcaccac
     8941 cagctcccaa ttgcatagca tcgagcttga cgccgaagtt ccagacccgg cgcaggaaac
     9001 ggtaggagcc ttcgacaccg gcgtcgttcc attccagcgt ggcttccggc ggcgaggtga
     9061 acatggtgta caggcgcgcg gtatcggcgc cgtatttctc gatcagatcc tgcggatcga
     9121 cgccgttgtt cttgctcttg ctcatggtgc ccacgccttc
//
LOCUS       DAIDFG010000104         9152 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_104_length_9152_cov_19.811147, whole genome shotgun sequence.
ACCESSION   DAIDFG010000104 DAIDFG010000000
VERSION     DAIDFG010000104.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 9152)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9152)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9152
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104_length_9152_cov_19.811147"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1821 a         2840 c         2815 g         1676 t
ORIGIN      
        1 ggcgtacgaa ctcaacatcg gttttgtgcc gatccgcaaa aaaggcaagc tgccgttcac
       61 caccgtgcag gaaacctatg aactggaata cggcagcgcc accgtcgagc tgcacacaga
      121 tgccgtcaag accggcgaca gggtgctgct gatcgacgac ctcatcgcca ccggcggcac
      181 catgatggcc ggtaaaaaac tgctggaaaa gctgggcgcc cgcgtcatgg aaggcgctgc
      241 gattgtggat ctgccagagc tgggcggctc cgccaagctg cgtgacgccg gtctgccgct
      301 gttcacgctg gtggatttcg ccggccattg agctcggtca atcgctatca attcaatagc
      361 cgtcggcgcc catctacaaa gggctggagg tcgatttgat tcaatattcc agaaaatgcg
      421 ggcagctgcg cccgcagttc acatcaggtc gccgtactgc gttgcactga gcgccggcgc
      481 cgagacaggc atctccggca tctcggtgtc ttcaaagccg gtgagcaggg tgtaggagtg
      541 cgggccgctg cgggttgaca cgccttttgc cacctgcgcc gcgccgccgt tggcggacgc
      601 ggcagccagg gcctgcttga aagcggtcac ttcgtcgtcc tgaatgggtt caaaacggga
      661 ggtgatcggc gccgcaacag ccgtctcgac ggcggcaacg gcagcgacag ccggctcggg
      721 taacgcggct ggcgcgctgt gcggctgggc gccgtgggtg ctgggccggg ggcggcccac
      781 cgacaccagc tcgttcatgc gccagtacac ggccgtcaca cggatgttgt agcgcgactt
      841 ggcgctctgg gcaatcatca cctcgatttc cgccagacga tccgtctggc cgccgaactc
      901 ctgcgccagg tccatcatga ccatgaactg gtcaccgcgc gggtccaggg acaacacctt
      961 gaatttgtag ctggccgaca ggacgccggc ccggatcatg gcctcgcgca cggcggcata
     1021 cagcagctcc ctcttggcat gccgctggga tttctgctcg ccggcgtgtt cgccggcttt
     1081 caggggtgtt ggcaccgaag ccctggcccg cgcgcctgcc accggccggg tccgctcatc
     1141 gcgcgtcagc cccgaactgt cgccgccagg ccgcgccttg ccggagacgc gctggtcgtt
     1201 gctggaggta cccgaaaacc aactgaaaaa tgacatcgtc tggcgcctgg aaaatgatgg
     1261 ctgcgcatgc agcgatcggg tggtgttgca ggaggcctgc aactttcatc ggtctagttt
     1321 actgattccc cttgtcaagg cagcccgcgc cgggcggatg caaggtaccg ccggtaagtc
     1381 ctggcgccca tccctgcaat aaatcacgtt atcgccgcct gaatgcacag ccagcgcctt
     1441 gtttacacct gcctttgtgg tgacgccacc actgaccacg acccagcgcc ctcaggtgac
     1501 cgccacacgt ttgggacggt tgtacaggcg cctggccatc accccgccca tggccagaca
     1561 cagttcgagc gcgatcgggc tgtgccggtt ggacagctcc ttgaaacgaa tcgcggtgag
     1621 acaccagagc tggcaggcgt tggtggcctg cacggtggcg ttgcgcggca ggcgcgagaa
     1681 aaaagcgcct tcgcccacca tggaacccgc agccaccatg gccagccgta cgcggccctt
     1741 ttcgtcctgg tagtgaacgc tcagcgtccc gctctcgatg aaatacaggg tctggtccgc
     1801 cgaaccctgc tcgatcagcg actgcccctg ttgcagcgcg aaaggctgta tgtagccggc
     1861 cagcacgtcc cactgctgcg gggtgagctg acagcgcagc gcgtcgctgg catggctgtg
     1921 cgccatcgca ttgatcagcc cctggatgtc aaagcggacc gaagtgggaa ggggtgcgtt
     1981 catgatgggt ggaagatacc cttcgcgggg accttgccgc aagggacgtt aacgcccgtt
     2041 tacacctgat acaccggcac gccacacgta atctgtccgc tgtcaccgtc ggcccaggcc
     2101 ggggtcaccc atcgttagcg tgctatttgc cgatgcagaa tttcgaaaaa atctcgccga
     2161 gcaggtcatc ggtcgtgaac tggccggtga tgtcgcccag ggcattctgg gcctggcgca
     2221 ggtcttccgc cagcaggtcc agcgcaggcg ccggtgcatg cagctgggcg gcggctcgcg
     2281 cgagttgcgc ctcgacctgg cgtagcgccc gtacatggcg ctcccgggcc atgaacaccc
     2341 cttcgggcgc ggcctgccag cccaccacct gcagcaactg ctgacgcagc tgctgcaggc
     2401 cagcgccggt tttggcggag atgagcacgc ctgaagaaac cgtggccagc acgtcggcac
     2461 cggccatgtc ggacttgttc cagacatcga tcaccggcgt gttggcaggc aacctgtcgg
     2521 tgatggcgtc agcgatttgg gcgtctgccg cgaggtagtt catggcaatg cggagatcct
     2581 cgggcgtgtg ctggcgcgtc aggtcgtgca ggaacagcac ggcatcggca ctctggattt
     2641 ccgtccaggc gcgctcgatg ccgatttttt ctacttcatc gacctcgggc agcaggctgt
     2701 cgcgcaggcc tgcggtgtcc accacatgca gggggacacc atcgatctgg atcagttcgc
     2761 tgaccttgtc gcgcgtggtg ccggccaccg gcgtcacgat ggccagctcg gcacccgcca
     2821 gcgcattgag cagcgagctt ttgccggcgt tgggctggcc ggcaatgacg accttgatgc
     2881 cttcccgcag gatggcgccc tgctgcgtgc gctgcagtac cgtggccagc gtctgctgca
     2941 gacgggtcag ctggccctgg gcatcggcct tctgcaggaa gtcaatatct tcctcgggga
     3001 aatcgagcgt ggcttccacc agcatgcgca ggtgcaccag ctggtcgagc agcgcatgaa
     3061 cctctttgga aaactccccg gacagcgaac gtgccgccga tcgtgcggcc gtttcggtgc
     3121 tggcgtcgat caggtcggca atggcctcgg cctgcgccag atcgatcttg tcgttcagaa
     3181 aagctcgctc ggtgaattca ccgggctggg ccaggcgcag gccggcgagc cgcggccgcc
     3241 ccgtggcggc atcattttct gccgccgcct ccaggcagcg tgccagcagc aactgaagca
     3301 ccaccggccc gccatgggcc tgcaactcga gcacatcttc gccggtgtag gaatgcggcg
     3361 ccggaaaaaa aatcgcgagc ccctggtcga tcggctggcc ggccgcgtcg ccaaaaggca
     3421 ggtaggtggc ctgccggggt gtcagcgcgc gcccgcacaa ggcctcgacc agcggtgcca
     3481 tggccttgcc tgaaatgcgg acgatgccca ccgcaccgcg gccgggcgca gtggtgatgg
     3541 cagcgatggg gtcgtggtga cgggccagca tgagggcacc atgttaaagg gccgcgaagc
     3601 ggcccttttt ttgtgtttac ttgaacttcg gcaaattgaa ctgcggcggc acacccatgc
     3661 gggtgttgat cacccactgc tgcgcgatcg acagcacgtt gttggtgatc cagtagagca
     3721 ccaggccggc cgggaagaag aagaacatca cgctgaagat cagcggcatg aaccacatca
     3781 gtttggcctg catcggatcg ggtggcgtcg ggttcagggc cgtctgcagc atggtggtca
     3841 gggtcatcac gatgggcagg atgaagtaag gatccggcga ggacaaatcc ttgatccaca
     3901 aaatccaggg tgcgttgcgc atctcgaccg agctgagcaa cacccagtac agggcaatga
     3961 acacgggaat ctggatcagg atggggaagc agccccccat ggggttgacc ttttcgtcgc
     4021 ggtacatctt catcatggcc tgctgcattt cctgcggctt gtctttcagc cgctcgcgca
     4081 tttccatgat cttcggattg accgccttca tcttggccat gctggcgtaa gccttggcgt
     4141 tgagccagta gaacgcggcc ttgagcagca gcaccagcgc cacgatggac cagccccagt
     4201 tctgcaggaa gccgtgcagc ttgtcgagca gccagtacag cggtttggcc agaatcgtca
     4261 gccagccgta gtccttgacc agctccaggc cgggcgcaat ggcttcgagc tgcttttcaa
     4321 tctgggggcc cacaaaaaac cgggcctcca ccgttttggt ggtgccgggc gccacagccc
     4381 ccagcggcgt gatcatgccc accgcataca ggttggtgtc gaccttgcgc aggaagatat
     4441 cgcgctggac gccgtcggcc agcatccagg ccgacgcaaa ataatgctgc accatggcca
     4501 cgtagccatt gctcgcctgt ttctcgacgt ccaccttgtt tttctcgatg tcgctgaact
     4561 cgaccttctg gtatttcttg gcctcggtat aaagcgccgg gccggtgaag gtggaataaa
     4621 acgaagactc gccgggcggc ttgttgccgt cgcgcaccag ctgcaggtaa agctgcggcg
     4681 aagccgcgct gctgcccgtg ttgaccacgt cgtgacgcac ggtcatgtca tagacaccgc
     4741 gcttgagcgt gtaggtcttg accagcttga caccgccgac ctcggccgac tcgaacttga
     4801 cttccagggt gtcctgcccg tccttgagcg tacgctcgcc gggaactgcg gtcatcaatg
     4861 tcttgtgggt cgggaaactg ccgccctcgg cgccggcaat caatccggac tgcgccacat
     4921 agacacgcgc cgcactttca tcaagcagca caaagcccga ggactggtcg accatgtcct
     4981 tgtgcttgag gaaggaggag tggaccagcg tgccgccttc ggtgtcgaaa gtcaggctca
     5041 gcacatcggt gttgacgacc agacgctcct tggtggccga cggcgtggtg gcaggcagcg
     5101 cacccggcgc cgaagccgcc gcggcaccgg ccaccggggc cggcacggca ctgccgccga
     5161 ccgggccggt gggcacactg gcggcactgg ccggcttgtt cgccggtgcc gtgctgctca
     5221 cggcgggggc gggcgacggg aaaaaggtgg ccttgttgcc attgaaaagc tgccactggt
     5281 cccacagcag gaccatggaa aaaccaaaaa tcacccacaa aatggtacgg cgaatgtcgt
     5341 tcatgacggc ttctttttag aggggatcga ggccacgggc gtgaccagac gggtgaaaag
     5401 ggaaggacgg ccggaaggcg gcggcaccgg atcgtaaccg ccgctgcacc aggggtggca
     5461 acgcgccacc cggtaaagcg tcaggtagct gccccgggcg gcgccatgct gctgcaaggc
     5521 ctgcagtgaa taagctgaac aagtcggctc aaagcggcac gacgatccca gccaggggct
     5581 gagcaggagg cggtaaccct tgaccagggc caccagtgtt ttctgcggca accggctcag
     5641 cgtcaccgcc gcacggcgaa gcgtggacag cgcgctcatg cggcctgcgc cccggcctgc
     5701 atcaaggcct gcacttcgct gcgtacggcc tgcttgagcg cctccgagct ggcgctgaca
     5761 aattccttgc gcgaaaagtc acgacgcaaa cggatcaccc acccggaacg ggacaggcga
     5821 ggcgaaaaat cagcgctcac ggtgtagatc tgtcttttga tggcgttgcg cgtgacggcc
     5881 cgtttggccc agcgtttggg caccatcgcc ccgacccaga catcctgaac gggaaacagg
     5941 tccggcgtgt catgggtctt gctgggcacg gtttgcgcgt tcatggcatc gagcgcattg
     6001 cggtgcaatg cgaaatgcgt tgttcttgca acaatgacgc cagcaaggac tgcctggaac
     6061 tggggtcggg ttttaagccg ctgcactagc gccaggcgtc cgcgaagcaa taggcctgct
     6121 ggacccggcg cttagacagc caggcgcttg cggcccttgg cgcgacgggc cgcaatcact
     6181 gcgcggccgc cacgtgtttt catgcgtgac aggaaaccgt gcgtacgggc gcgcttgact
     6241 ttggaaggtt ggtatgtgcg tttcatgatg ggtcctacaa gctacaaaaa gctcccttgt
     6301 gcaaaatctg aaaattctgc gattcgatcc tggcggctgt acaacacaac caactctgca
     6361 agtcagcagg gcccgcaagg cgtaaacggt gcttgaaaac aagcatcgcc acggcgcgcc
     6421 gacccgctat gaatcaggga aacccacgat tatcgcagat ttcccaacgc tggcaacaag
     6481 ttaggggccg cattgccggt aaatgctgag atccccctgt caacgccggc gccagggccg
     6541 cgcagcgacc gcccggcgct acctaggtta aaacccgaag tcctttttgt gtggataact
     6601 acgccacacg actacaataa cgggtgccaa aaattaataa tatccacaag caaaagcatg
     6661 ctggccaatg cgactaccca gcccccggac accatgcgtg agggagccgt tcacagttca
     6721 agccaggact tgtggcaaag ctgcgtggac catctggcgc aggaactgcc cgagcagcag
     6781 ttcaacacct ggatccgtcc gctggtggcc gaggtcgccg gcgacctgtc caaagtcacc
     6841 attcaagttg ccaaccgctt caagctggac tggatacgcg cgcaatatgc cggccgtatt
     6901 tctgccctgc tggagaaggt ttccggccac agcattcatc ttgagttagc gctcgctcag
     6961 cgcgaacccg gcaccaggtc tgcacctttg acgcgcagct tcgaaggtaa cggcgtcacc
     7021 gagccggctg gcttgggcgt cgcagaagac gctgccggtg cgccgttcaa gagccggctc
     7081 aacaccgcgc tgaccttcga caccctgatc gagggcacgg ccaaccgcat gggccgtgcc
     7141 gccgcgatgc atgtggccag cagtctgggc cagctttaca acccgctgtt catctacggc
     7201 ggcgtcggcc tgggcaagac ccacctgatg cacgccatcg gcaacaagct gatggtcgac
     7261 aaccccgccg ccaaagttct ctacatccac gccgagcagt ttgtgtcgga tgtggttaaa
     7321 gcctaccagc gcaagacttt tgacgagttc aaggagcgtt atcactcgct cgatctgctg
     7381 ctgatcgacg acgtgcagtt cctcgccaac aaggaccgca cccaggaaga gtttttcaac
     7441 gcctttgagg cgctgctgac caagaagtcg cacatcgtga tgaccagcga cacctatccc
     7501 aaaggcctga cggacatcca tgagcgcctg atctcgcgtt ttgactcggg tttgacggtt
     7561 gccatcgagc cgcccgagct ggagatgcgc gtggccatcc tgatccgcaa ggccgaggcc
     7621 gaaggcacgg tcatgcccga agaggtcgcg ttttttgtgg ccaagaacgt gcgctccaac
     7681 gtgcgtgagc ttgaaggcgc tctgcgcaag atcctcgcct actcccgctt caacctcaag
     7741 gaaatctcca tccagctggc ccgggaagcc ctgcgcgacc tgttgtccat ccagaaccgc
     7801 cagatatcgg tcgagaacat ccagaagacc gtggccgact attacaagat taaagtcgcc
     7861 gacatgtatt cgaagaagcg gcccgccagc attgcccggc cgcgccagat tgccatgtac
     7921 ctggccaagg aactgaccca gaagagcctt cccgaaattg gcgagctgtt tggcggccgc
     7981 gaccacacca ccgtgctgca cgccgtgcgc aagatggcgg ccgagcgcca gcagatgacc
     8041 gaactcaacc agcagctgca cgtgctggaa caaacattga aaggctgaat tgaatacttt
     8101 ttcagcaatt tcccaccggt ttcaggcttg cctgacaacc atggggacaa cttttgaaca
     8161 actgatgact tatccacagg ggaagtggat ttccggaagt tgttcatgtc gagcggcaga
     8221 gtagagggtg gtctgaccac aaggttaaac aagcgccaag tagttgatca aaaagaagaa
     8281 aatagggctg tccacagaaa cgtgcttccc ttatctacta ctactaattt gaattaaaga
     8341 aataagaggt taagacagtg atcgttctca aagccaccca ggataaagtt ttgtcggttc
     8401 tgcaatcggt cgcgggcatt gttgagcgtc ggcacacgtt gccgattctg gccaacgtgc
     8461 tgatccgcaa gaccggcgcg cagctgcagc tgacgaccag cgacctggaa atccagatcc
     8521 gcacgacagc cgagctggac ggtgacacgg gcaacttcac gaccaccgtt ggtgcacgca
     8581 agctcatcga catcctgcgt tccatgccca gcgaccagac ggtgtcgctg gaatcggcgc
     8641 agaacaagct gatcctcaag ggtggcaaga gccgcttcac gctgcagacg ctgccggccg
     8701 aagactttcc gctggtgcag gaagccgcca acttcggccc cagcttttcg gtgccgcaaa
     8761 aggtgctcaa ggagctgctg aaccaggtct cgtttgccat ggctgtgcat gacatccgct
     8821 actacctcaa cggcatcctg ttcgtcgccg aaggcaagca gctcagcctg gtggccaccg
     8881 atggccaccg cctggcgttt tcgtcggcca cgctcgatgt cgaggtgccg cgccaggaag
     8941 tgatcctgcc gcgcaagacg gtgctggaga tgcagcgcct gctcagcgac aaggaaggtg
     9001 cgatcgagat gcagttcgcc ggcaaccagg ccaagttctc gttcgaaggc atggagttcg
     9061 tcaccaagct ggtcgagggc aagttccccg actacaaccg cgtgatcccg aagaaccaca
     9121 agaacatcat caccctgggc cgcgtgccgc tg
//
LOCUS       DAIDFG010000105         9101 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_105_length_9101_cov_17.317599, whole genome shotgun sequence.
ACCESSION   DAIDFG010000105 DAIDFG010000000
VERSION     DAIDFG010000105.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 9101)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9101)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9101
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105_length_9101_cov_17.317599"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1691 a         2792 c         2843 g         1775 t
ORIGIN      
        1 gtgatgtgct gggcctgtat tccaacatcg cgattgcctg gatgatggcg gtggtggctg
       61 acctcgtcat caacaaaccg ctgggcctgt cgcccaaggg catcgagttc aagcgtgcgc
      121 acctctacga catcaacccg gtcggtgtgg gggcgatggc actggcctcg ctgctgtcgg
      181 tcaccgcgca cctgggcctg ttcggcccgc tggcccaggc tttttctgcg gtcattgcgc
      241 tgggcacggc ctttgtcacc tcgccgctga ttgcctgggc cacgaaaggg cgctattaca
      301 ttgcccgtca gggggaagcg cccgaggccg atgcccaggg caattacaaa ccctatgccc
      361 tcaagcgctg cgtgatctgc gagcgcgact acgaaggccc ggacatggcg cattgcccgg
      421 cctaccaggg gcccatctgt tcgctgtgct gcacgctgga tgcgcgttgc ggcgatttgt
      481 gcaagccgca ggccagcctg tcggcgcaat ggtcggccac gctgcgctgg ctgctgccgc
      541 gccgtatctg gccgtatctg gacaccgggc tcgggcactt cctgctcttg atgctgatca
      601 tcgtgcccct gctggccagc gtgttcggcc tgctttacca ccaggaaatg cgcgccttgc
      661 tggacctgca ggccgcctcc gaatcggcgc tgcgctccgg cttcctgaag gcctacatgg
      721 cgctgctggt gattgccggg ctggtggcct ggtggctggt gctggcgcac aagagccggc
      781 aggtggcgca ggaagagtcg aaccgccaga cgcacttgct gatgagggaa atcgagtcgc
      841 accgccagac cgacgaggcc ctgcagcagg ccaaacgggt ggcggatctg gccaaccagg
      901 ccaagagccg ctacatcagc gccatcagcc acgagttgcg cacgccgctc aacagcatcc
      961 tcggttatgc gcaactgatg ggcgaagaca cgtcgattcc gctgcaccgc cagcaggcgg
     1021 taggcgtcat caagcgcggc ggcgaacacc tgctctcgct gatcgaggga acgctggaca
     1081 ttgcgcgcat cgaaagcggc aaactgacgc tcaatgtcag gcccatgcac tttgccgact
     1141 ttgtgcagga gatggccggc atgttcgagc ttgaggcgca agccaagggg ctgggctttc
     1201 acttcgagtc cgaaggcacg ctgccggtgg tggtgcgtgc cgacgaaaaa cgtgttcggc
     1261 aaatcctgat caacctgctg ggcaacgccg tcaagttcac ggccaggggg cgcatcaacc
     1321 tgcgcctgaa atatgcgcgc gagatggcca gcatcgagat agaggacacc ggcccgggcc
     1381 tgtcggcgca ggaaatcgag cgcatcttcg agcccttcac gcgggcgtca acacccggcc
     1441 ccgctgcacc gggcgcgggc ctggggctga cgattgccaa gatgctgacc gacctgatgg
     1501 gcggcgaact cacggtggcc agcacgcccg atgtcggctc ggtgtttcgc gtcaagctgt
     1561 ttttgcccga agtgcggctg gcgcccggcg cggcgctgcc ggcgccatcg tcgcgtgcgc
     1621 acaaggcacg ttacggttat gcggggccgc gccggcgcgt gctggtggtg gacaacgagg
     1681 aggccgaccg cgaactgctg gtcaatgtgt tgcagccgct cggtttcgaa atacgcacgg
     1741 ccgccagcgg tcacgactgc ctggacctgc tggccgccgg ctaccggccc gaggtcatcc
     1801 tgatggacct ggccatgccc ggcatcgacg gctgggagac cctgcgccgc ctgcgtgcgg
     1861 cgggccatgc cgatattcac ctggcgatcg tgtcggccaa cgccttcgac aaggggctgg
     1921 acaacgacgt cggcatccgt ctcgaagact tcatcctcaa gccggtgcgc cacagtgagt
     1981 tgatcgactg gctggagcgg cgcctggcgc tggtctggct ggaagcgccg ccgcaggcgc
     2041 cggccgcacc ggcgccggct gcgctgccgc gcatctaccc gcccgcggcg cagctcgacg
     2101 ccttgcgcga ggtggtcaac ctcggttatt ttcgcggcat catgaatacc ctcgacgaga
     2161 tcgaaaaatc acagccgcaa agcgccgggt ttgccgccga aatgcgcggc ctggcgcgcc
     2221 agttccagtt tgaaaccatg agccagcagc tcagcgaggt gccgacatga acgaccgcac
     2281 actcgcgcac acgctggacc gcgccaacag cgacgtggtg ctgatcgtcg acgacgtgcc
     2341 cgacaacctg tcagtgctgc acgacgcgct cgatgaatcg ggctacaccg tgctggtggc
     2401 cacccacggc gaagccgcct tgcagcgcgc tgcccaggcc ctgcccgaca tcgtgctgct
     2461 cgacgcgatg atgccgggca tggatggctt cgaggtggcc aagcgcctga aggccgatgc
     2521 gcgtaccgcg cacattccca tcatcttcat gaccgggctg accgagaccg agtacctggt
     2581 cgccgcgctg gaatcgggtg gtgtcgacta tgtgaccaaa ccgatcaagc ccaaggaagt
     2641 gttggcgcgc atcggtgtgc atttgcagag cgcgcgcgaa aagcgccagg cgcgcaacgc
     2701 cctggacgcc tttggctacg ccaccatcac ggtgcgagcc aatgacggca agctgatgtg
     2761 gcagacaccg ctggcgcgcg agctgctgca gcagtattac ggcaccagcg cgccgcagac
     2821 gcctgcagcc gtgctgacct ggctacggcg caacctggcc gatgccgagc ggcagatcga
     2881 gccgccgcgc ctggtgtgcg agagcgctgc ctcggtcgcg cgccggctga cctttcgact
     2941 gcaccagcag actggcgacg acgactggct gatcatcatg cgcgaggagt ccgacgacac
     3001 cgtgatccag gccatgagcc tgagcttcaa gctcacggcg cgcgaggctg aggtgttgta
     3061 ttgggtggtc aaaggcaaga tcaaccgcga catcggcgac atcctgggca ccagcccgat
     3121 gacggtgaaa aaacacctgg agcgggtgtt tgccaagctt ggcgtggaaa cccgcacggc
     3181 cgcggccggc atggccatga gccggatccg gctgctgcac ccgcagtttg agcaatagat
     3241 agagccccca cgttcgctcg ctgcgcgtag ctctctgccc cccgagggtg cgctgcgcct
     3301 gcggactggc agagccagat ccgcggcccc tgcttgaaaa tacctcctcg gagggtgttc
     3361 tgcgccttgc ttcctgcgtc tcagtaatgg ctttgtgcgc gcaggcgtgg taacggttac
     3421 aataagcgat tcggtaaagc atcagctttt tacatcgaag cccccttacc acgcggcttc
     3481 cttctcaaat gcggcgcttc caagagcccg aattcacagt gttttcagtg cccctacagc
     3541 gctgaaccct ctcaaacact gttccatttc cggcgagcac ctcccccaac ggtgcccacg
     3601 caaggctcaa ccagccagtc ctttatgttg taaagcttgg cggtcaggac ccgaacacgc
     3661 gcgggcttgc ttgcaagccg ttatttcctt atttatcaag ccgggaaaaa tacccggcat
     3721 ggactttatg aacatcgatc acaccaacat cgcggtgggc aagtaccttg tctccccgct
     3781 cgccaaaccg cagggcgaag gcctttacgc ggcgtcggtc tccatccgct ccgggcgcgg
     3841 cagtgccacg catgaccgcg tgctgcggtt cagcgggctt tttgacagcg ccgcggctgc
     3901 cgtgcagtac gccacggaac acgccctcgg ctggattcac gagcgcacgt cgccggtttg
     3961 cgcctgaacg gctttgcccg atcacctgca cgacagattt ttttcaaatt cccaaggagt
     4021 tttcatcatg gccaaagaag aactgattga aatgcacgga ctggtcaacg aagtgctgcc
     4081 ggactcgcgc tttcgcgtga cgctggacaa cggccacaag ctggtggcct acacctccgg
     4141 caagatgcgc aagaaccata tccgcattct ggcgggcgac caggtgtcgc tggagctgtc
     4201 gccttatgac ctgagcaagg ggcgcatcac gttccggcac attgccggcc gtggcccagg
     4261 cccttcgcgc acccactagt cgcaagccac tcttttatag atcattttgg ctgccagtcc
     4321 ttatcgggcg ggcggcaata gctattttat tgatagcaat agaacgccga ctcagggccg
     4381 gggcgcgttg tccgcagaac tcggtggccg cgccatccag gcgaacatcg acgagcgcat
     4441 cgcttcctca accgtcatcg caatcggttg cacccattcg acaggcacaa aaacggtgta
     4501 gccgccgatc atgtagccca tcggcaaata caccgccaca cgttcccccg gcagaaagcc
     4561 cgcgggcaga tccgaaaaac tgcggcgcgt gatcagaccc acaatttcga gcccgttgtc
     4621 cggcatgcgc aggatcacca cgctttgttc gccctgtttg cccgtgggcg aaaagtagtc
     4681 ggcaaaactc ttgagcgacg agtaaatgct tttgacgaca ggcaggttgg tgaacggcag
     4741 ctcgacaacc agcagcgcct tgcgcacgcg cggtttggac accaggtagc cgatcaacac
     4801 aatgcccagc atgccgacca ccagccccat gccaggaatg tagaagtcgc cgaacagcgg
     4861 ccgcaggatc cacaacgtca gggtctccat ccatgcgagc atgatgaaga gaaggtacag
     4921 ggtcagggcc agcggcagga tggtgatcag cccgcggaag aaatatctgt agagcttttt
     4981 catggtggtt tcaaaaaggg aaaaacgggc cgggcagggt ggtgagcgaa gccccccaat
     5041 ataccgaagc tctgtggctc gtcccgcgac caggtgtggc gttcaacgct gccgtgtagg
     5101 tctggcccta cctttgctgg ggtccgcttc cggcactcgc tcctgcgtga ggtgcttatc
     5161 tttgggtttc gtacttaaca aggagaaatc acatgcccat cattgcctgg ttactcggtg
     5221 taccgatcag cgtcatcatt ctcttgatgt tgttcggcgt tttttaaatc cgggaggcac
     5281 ttcatgcttc gcaaactgct tgttttcgcc atcacctccg ggctggcggc caggctgtat
     5341 cgccgctacg ccagcaggcg caacccgcct gtaacgccgc tcaggccagc tgcgacgcgg
     5401 cgtcggcgtg ccggggcgca gagcgattcg aattgacacc ctgccccggc ctcacccgcc
     5461 ggggcatttc catatagcac ccaccacggc gcgctgctca ttccatcttc gacaccggtc
     5521 caaagcaggc ctggagagag gccttctcgc agaccagcat cttgtcgcgc tgcggactga
     5581 actgaatgaa gcgggtgatc tgttcacccg gcccctccag cagcgccccg gaacaatcct
     5641 tcttgtcgtt ttgccggacc acggtctcca caagtttgta gaaaccgttg gcgtccggca
     5701 gcttcgcgac ctcgtatgtt ttttcggcca cttcctgccc gctggtgctg agcaccgtgc
     5761 ggttgctgcg gtactgccac gtctcggtac agctgccccc gaagagcgac caggaccagg
     5821 tgcctgtcag cgggggagcg ttcgccggcg cggattctcc ggcggcctgc agcaggggag
     5881 cggccatcag caggcaggca gtaaaaagca gttttcgcaa tcggggcttc ctcataggca
     5941 agggatggag ctgagtggcc aatgctactc ccttgcagcc ggccccttgt caggcgccgg
     6001 ccggacaacc cgcgcagcta ttgaggaaag gcgtggctat agtggggtat gttcacgctg
     6061 aaggagaccc catgaaaaag ttcaccctct ccccgttgtt gttgtcgttg ttgctcggcg
     6121 tgttgtcacc cgtccacgcc cagagcatcg gcgaggtgga caccgtgttc aagttcatcg
     6181 ggcccgatca caagatcgtg gtcgacgccc atgatgaccc caaggtcagg ggcatcacct
     6241 gttacgtttc gcgggccaaa acggggggca tcaagggggc cgtgggtttg gccgaagaca
     6301 aatcggaggc gtccattgat tgccgccagg tggggccgat ttcgtttgtc aaaccgctgc
     6361 cgcagcagga agaggttttc agcgagagga tatcgctggt tttcaagagg atacgcatcg
     6421 tacgcatggt cgatgccgcc cgcaacaccc tggtgtacct gacgtactcg gaccgggtga
     6481 ttgaaggctc gccgcaaaac agtgtcgcag cggttcctgt tgatctcgcc atgaaaattc
     6541 cgttgcagcc ttgatgcctc ccggcggtgc gagggggggc acgcgactgc ggccttttgt
     6601 cagcgggcgt tttccaggta ccagtaacgg cgtttgcgca ccggtgtgat ggttggcgtc
     6661 accttcgagc cttcaatgcc cgagcctttg ctgtttggcg cgccgatgat gaaaggcact
     6721 cgcttggtag ctgtttcacc ggtgatgggg gaggtgtagg aaaccgtcac ggtgccagtg
     6781 actggactcg gcggcaggcc gccgccgacc agtgtctgcg cggccgctgc cttgcagaac
     6841 agcggggccg agtagacctt ggcaatgccc aggttggcgc tgcacgcgtt ggtactcact
     6901 ggcgccggct gattggtact gaagtaagtg atgccgccca cggagaccgg cgcattcaca
     6961 atcttctcgc cagtggttgc catgggaatg tagcaaccat tggtcaggct ttcgctcgaa
     7021 ccgaccaagc cgagactgct cgaagagatg gctgtggggg cgctgctggg cgtgcccttc
     7081 ccggtcagtg tgtcgtagag ggtgaaaaac gcatcggtgc tggtggtggc cagcggcttc
     7141 tcacggtcgc ctgagcccac cagaaccgcc gtgtagttgt tcgtgacaac aacatcaggc
     7201 ggatagaaaa attttcgcgt accactgcca gagagcgccg cgagctggta gatgccccaa
     7261 tcgctgacgg ccgaggttgt ggttgcactg accgttttct ccaggtcaat cctgtataca
     7321 ttgccaccca tgtccacggc gtaggcgcgg tccacataac cgtcaaagtc ggcgtccacc
     7381 agggaaacgt ctgcgggaac actgcgcgtg gtatcgaatt tcttcagcag ggcgcctgtg
     7441 atggcatcaa gtaccagaac agcattgccc atggtcgtcg tgccctgagg tgaaacatct
     7501 tcggcagccg cgtcataccc tgcgcccatg atgaggactg ggttggcgtt gccccgtatc
     7561 ctggcgacct tgggctcgga ccatgtctgg ccgagaacag aaatgtccgc ctcggttttt
     7621 ttccagagaa atttcggcgc ggcaggcgtg gtgacgtcca gggcatagag aatgcgtccg
     7681 ccgcggcgca tggcgatgta aagatagacc ttgtcgttgg tgccgtcggc cagtaccttc
     7741 tggtaaatgc cgataggccc atcaacaaaa tagtcccgcg atgtaggcgt cagggacgtg
     7801 gtcggtgttc cagccacagt ggtcgacagg cgtatttttg gcgtattgac gcgcagcctg
     7861 ttcagttttg aaaagtgttc ttccggtatg aaactccaca gttcattgcc tgcgcccgtg
     7921 ccggtctggt tgccattgac ggcacgcaag gtgccatcgt tggaaccgta gaacaccacg
     7981 acaccggtgg tgccgccgta gttcaccacc gctggacggg aatgcaggac gtcgccatgg
     8041 gcgcttggac gaaccgtgac tgtcccgccg gggccctgtt catcgcccgc gttgtccgcg
     8101 cccctcaccc agttgatcag gttgtcccgg tccgtggtgc tggatactcc cagcagggac
     8161 gtggtgatgc cgctcgtcgc tgtactgaac tgtgcggcag cggtgctggc gagattggtg
     8221 ttggctgcac aagcctgaca ggtgtagaca ttgcgggcag tctgggtcgt ggcataggcc
     8281 gaacggatcc gctgggcgac accgcctttt tccacgacct ctccgtcggg cgagtcggag
     8341 cttgacagcg gcgtccccag ttgctggttc acccagaagg tactgtcggt ggtccagtac
     8401 gaaacagctg ctggcgacag aaaaccggtt gcaccgctga tcgccggatt gccgtttttg
     8461 tcgctcagga acaggctgtc agtggtgggg tcgtagccga actggtattg tttcaggttg
     8521 ccgcgccaac gcggaagggc gtcgccatcc ggtcgaaaca tgcccatgaa gatctgattc
     8581 aggaaagtgc cgcgagcgtt cacgctcacg ggcaggctgg cagatgaaaa cacactgtct
     8641 accgcctgga tctggttgaa tatctgggtc aaggcattgc tgatttccgc gccggcaccg
     8701 gtgcctgaac tgacggagaa atactcgcca ccgctggcgc tcgccatgct cctgagcatg
     8761 gctgaccagc ccgggccctg tccgttcgtg atcttgtcca cgtccagcgt ataggtggca
     8821 atgttttgcg gacttttctt catgaagcgc gcccattcat ccgatacacc ggactgggag
     8881 ccgctgggag agaggttcag gtccgcaggc cgggtcagcc ccagggcagt gtaggcagcc
     8941 gacagcctgg tggatgcctc gctggtatca gagttattgt cctggactgc gccgttgctg
     9001 atgtaaatga tgtagttgcg ctggcagtca ttgagcaccg ggctgacgta gggtgttccg
     9061 gacttggacg tcaacgcgtt gcccgtgatc gcatagacgg c
//
LOCUS       DAIDFG010000106         9076 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_106_length_9076_cov_17.658020, whole genome shotgun sequence.
ACCESSION   DAIDFG010000106 DAIDFG010000000
VERSION     DAIDFG010000106.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 9076)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9076)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9076
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106_length_9076_cov_17.658020"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1420 a         2981 c         3074 g         1601 t
ORIGIN      
        1 gaaggcctgg ccatgttcgg cgacgcgaat tttgacgtgg tgctggagat cgacaccctg
       61 cagcacctgc gcaatgccga ggtcatgctg cgcgagacgg cgcgcgtggg ccgcaccggc
      121 atcgtcgcct ttcccaattt cggccactgg ccgaaccgcc tggcggtgct gcgcggccgc
      181 atgccggtca ccagggtgtt gccctaccag tggtacgaca caccgaacat ccgggtcggc
      241 accctgcgcg attttgcggc gctggcgctg aaaaaccggc tccacattct ggacgccttt
      301 ggcctgcagg atggccggga agtccgcttc tggccgaacg cccgcgccag ccgcgcggtg
      361 ttcaagttcc agcacggctg aaaatccggg tccgctcatg accgctgacg ccgacgcccg
      421 gattgcatcg cctgccggcg tcccgtttgc cgaagccttc cggttctggc tcaagctcgg
      481 tttcatcagt ttcggcgggc cggccgggca gattgccatc atgcaccgcg agctggtcga
      541 gcagaaacgc tggatctcgg agaagcgttt tctgcacgca ctcaactact gcatgctgct
      601 gccggggccg gaggcgcagc agctggctac ctacatcggc tggctgatgc accggacctg
      661 gggcggcgtg gtggcgggtg cgctgttcgt gctgccgtcg ctgtttatcc tgatcgcgct
      721 gagctggatt tacgtggcgt ttggcgacgt gccgctggtc gccgggatgt ttgacggcat
      781 caagccggcc gtggcggcga ttgtgctgca ggcggtgcac cgcatcggca gcaagtcatt
      841 gagaaatccg gccaaagccc ccgttccttg ggcggtagct gctatcagtt tcgtagcgat
      901 tgcgtttttg catgtgcctt tcccctgggt ggtgctgggc gccgcgctga ccggctggct
      961 gtgcgcgcgt tttgcgcccg ggcagtttgc tgtcggtggt ggacatggcg gcacggtgct
     1021 ggtccatgtg cctgcgctga tcgacgacca cacgccgacg cccggccacg cgcgcttcag
     1081 catggggcgg ctggcccggg tgctgacggt gggcgccgcg ctctggctgt tgcccatggg
     1141 ggcactggtc gcctggcagg gctggcacgg cgccttgacg cagatgagct ggttttttac
     1201 caaggcggcg ctgctgacct ttggcggtgc ctatgcggtg ctgccctatg tctaccaggg
     1261 cgcggtcgag cagttcggct ggctcagcgg gccgcagatg atcgatgggc tggcgctggg
     1321 cgaaaccacg ccgggcccgc tgatcatggt ggtggctttt gtggcttttg tcggtggctg
     1381 gaccaaggag gtgctggggc cggacgcatt gttccagggc gcggcgctgg cggcgctgct
     1441 ggtcacctgg ttcacctttt tgccatcgtt catttttatc ctggcgggcg gaccgctggt
     1501 ggagtccacc cacggcaagc tccatttcac cgcgccgctg gcggccatca cggccgccgt
     1561 ggtcggcgtg attgccagtc ttgctctgtt ttttatagcg catgtcgctt tcccgacggg
     1621 cgcgacgggc ggttttcctt ccaatgtggc atggccggcg ctggccatca tggccgcggc
     1681 cagcgtggcg ctgctgcgct acaaggtggg tgtgattccg gtcatcgccg ccagcgcggt
     1741 ggccggcttg ctgctgcgcc tgggcggcct ggcctgaggg cggcgtgcca cacgcgccgc
     1801 gttatgctcc agcccatgca aggccctccg gaaaacacac acacagcccc ggcgctgaag
     1861 ctgtttgagc ggctgttcgg ccactgggtg caccaggtga cgccggcgag cctgcgcgcc
     1921 gacctgatgg cgggcctgct gggcgcggtg ctggtgttgc cgcagggcat tgcctttgcc
     1981 acgctggccg gcctgccgcc cgaatacggc ctgtacaccg ccatcatccc ctgcatcatt
     2041 gccgcgctgt tcggctcgag ctggcatgtg atgtcgggtc cgaccaatgc caattcgctg
     2101 gcgctgtttg ccatgctttc gccgctggcg ctggccttca gcccgggcta catccagatg
     2161 gcgctggcgg tcacggtgat ggtgggcgtc atgcaatggc tgattggtgc gctgcggctg
     2221 ggttccctgg ccaacttcat ctcgcctgcg gcgctgttcg gtttcacctc gggggcggcg
     2281 gtgttgatcg cggtgtacgc cctgcctgat ctgctgggcc tgccggcggc gaccgaccat
     2341 ggggcggccg cggtgctgct gcatgtggcc acgatggtcc ggtcggtgca gccggcggcg
     2401 ctcgccgtgg ccggcgtgac ggtggccgtg gccctgctgg tgcggcggct gcgccccagc
     2461 tggccgtcca tgctggccgg gctggtggcg gcgacggccc tggcctgggt ctggacgacg
     2521 tatgtcagcg caacgccgct gccgctgcgc acagtcgggc agatcaccac gccgtggccg
     2581 aaatttgcca tccccagcgt gagctggtcg gcgctgtccg agctgctggg gctggcgttt
     2641 gcgctgacca tcgtggcgct gggtcagtcg atctcgatcg ccaaggcggt ggccgcgcgc
     2701 tccggccagc gcatcgacgc caaccgcgag ttccgcggcc agggcctgtc caacatcatc
     2761 ggcggcttct tttcgtctta cgtgtcctgc ggttcgctga accggtccat gccgaatctg
     2821 gaggccggcg cgcgcacgcc gctggcggcg gtgttctcgg ccctgctgct gctgggcctg
     2881 gtcgccgtca gtgcggcgct gctggcgcag atcccgatgg cggcgctcgc ggcgctgctg
     2941 gtgctggtgg ccgtctcgct gctggacgtc gggcgctggc ggcagttgtt ccgcctgagc
     3001 cgaaccgaat tcggcgtggc gctggccacg ctggtggcga ccgtcagcat ccggctggag
     3061 atcgcgatcc tgctgggcac gctgttgtcg ctgatgtcct ttctgtaccg cacgtccaaa
     3121 cccgccatgc gcaccatggg cttcgacagc cgggcgccgg accggcagtt tgtggtgctg
     3181 gccgacagcg caacgccact gccggaatgc ccgcagctca agctgctgcg catggagggc
     3241 gaggtgtatt tcggcgcgac ccagcatgtg gccgacaccc tgcacgcctt gcgtcatgcg
     3301 cgccatccgc aaaaacacct gctggtgatg ggcaaaagca tgaacttcat cgacctggcc
     3361 ggtgcccagc tgtgggaagc ggaactcaac gcacgccggg cgatgggggg cgatttgtac
     3421 tttcaccgcc cgcgacccga agtcatccag atgtggcaga ccaccgggtt cacgcggctg
     3481 ctcggacccg agcaccagtt ccccgacaag cgcagcgcga tcgccacgat tttcacgcgc
     3541 ctggacccgg ccatctgccg gtcatgcacg gcgcgtattt tcgaggagtg ccgcagcgtg
     3601 cccgggccgg atcccgtggg cgacgcagcg cgccctatgc cggtggcgtg aagcccaggc
     3661 gctgcagcag accggtggcg gccgccggcg ccttctcctt ggcgccgctg atgaaaaaca
     3721 caaacacgtc tttgggcgcg cccttgtctt ttgcggcctc cacccgcggc aggtccgacg
     3781 gctcgccgcc ttgcgcccaa gcctgggccg ccgctgtcca ggcgtcaagg gcgttgggtg
     3841 cataaccgtt cttgagcctg gcatcactgc gcatcagccg ggcatacacg aaatcagacg
     3901 tcaggtccgc aaaggacgga aagtcgggcg aatcggtgaa caccgtcgcg cagtggtgct
     3961 tgcgcgccag tgccaggtag tccggcgtca tgaagctggg gtggcgcaca tcgagcacgt
     4021 ggcgcagctg gcggccatcg acggtcttcg gtagcagcgc caaaaaagcc ccgaaatcct
     4081 ccgcatcaaa caccttggtt ggcatgaact gccagacgat ggggccgagc ttgtcaccga
     4141 gttcgctgat gccgctgccg acaaaccgtt cgatggaatc accggcttcg gccagaaccc
     4201 ggcgattcgt ggtgaagcgg ctggccttga gcgagaacac aaagccgtcc ggcgtttcat
     4261 cgcgccattt ggcaaaggtc ggcggcttga aggtgctgta gtaggtgccg ttgacctcga
     4321 tggcggtcag ctgccggctg gcgtaaccca gctcctggct ctgcggcagt ctggccgggt
     4381 agaagttgtc gcgccagggc gcgtaggtcc agccgccaat gccgacgcga atggcgggaa
     4441 gaggatttcc ggatgttttc aaggcagata ctcccgcaaa caggggatga cgttttcgcg
     4501 atggtcccgg acaatgccct gatgtgcggc gttccccgaa ggacagtctg gcacgatgtg
     4561 cgcagcacgg ttacagtgac agttcagccg gaccgtcccg ctgaacccca tttcccggag
     4621 tgtattcatg aacgcccccc tgcatcgaga aattcccgcc agcgtgcttg acgccgccca
     4681 ggccctgtcc caggtcagcg cccagtggga cggcgacatc gtcaagcaga tcaccgacta
     4741 catcaccatt cccgccaagt cgccgtcctt tgatgccgac tgggccggcc acggccacat
     4801 cgaaaccgtg ctgcgcaatg ccgccgcctg ggtggaagcg caaaaggtcc agggactgac
     4861 gctcgagatc gtccggctgg aaggccgcac gccggtgctg tttttcgagg tgccggcgac
     4921 taaagcgggt gccacggaaa ccgtcttgat gtatggtcac ctcgacaagc agcccgagtt
     4981 caccggctgg cgcaacgacc tgggcccctg gacgcccaag tacgaagacg gcaagctgtt
     5041 cggccgcggc ggcgccgacg acggttatgc ggtgtacgcc agcatcgccg cgatccaggc
     5101 cttgaaggcg cagaacgtgc cgcacccgcg catcgtcggt ctcgtggaaa cctgcgagga
     5161 aagcggctcc tacgacctgc tgccttatgt cgatgccttg cgcacgcggc tcggtgacgt
     5221 gggcctggtg atctgcctgg actcgggtgc cggcaactac gaccagctct ggctgaccac
     5281 ctcgctgcgc ggcatggcca gcggcgtgct caaggtcgag atcctcaccg aaggtgtgca
     5341 ttcgggcgat gccagcggcc tggtgccctc gagctttcgc atcatgcgcc aggtgctgga
     5401 ccggcttgag gacagcaaga cggggcgcct gctgccgcaa agttttcact gcgagatccc
     5461 ggccgagcgc ctgcagcagg ccaaggccac cgcgcaaatc ctcggcgacc agatctacaa
     5521 gcgtttcccg tgggcgcatt acgactgcgg cggcgccacc acgtttgcgc tgccgaccac
     5581 caccgacccg gtcgaggccc tgctcaaccg cacctggaag ccgacgctga gcgtgaccgg
     5641 tgccgacggt tttcccgaaa tgggcaacgc cggcaatgtg ctgcggcctt acaccgcctt
     5701 caagctgtcg ctgcgcctgc cgccgctggt ggatgccgcc agcgcagtgc aggagctcaa
     5761 gaccctgctc gaagacaacg cgccctacca ggccaaagtc acttttgaaa gtgccggcgg
     5821 cgccaccggc tggaatgcgc ctaccacctc accctggttc gagcaggcgc tcaacgaagc
     5881 ctcacagact ttttatgggg cgccttgcgg caccatcggc cagggcggca cgattccgct
     5941 gatgaacatg ctgagccagg gttttccgaa ggcacagatg atggtctgcg gcgtgttggg
     6001 tccgaagagc aatgcacatg gccccaacga attcctgcat gtgccgtatg ccaagaaaat
     6061 caccgcagcc gtcgcgtccg tcatcgcgcg catgccttga accggcaagt catggatcat
     6121 cccctgctgg cgccgttcac ggcccgcgac ggcgaaaacc tggcgatcta cgagtggccg
     6181 atggaggcct ggcaggccga caacgccctg ccgccgcgcg cggtggtgct gctggtgcat
     6241 ggcctgggcg aacacgcgag ccgctatgac catgtggcgc agcacctgct cgactggggt
     6301 tttgcggtgc gcgcctacga tcagcgcggt cacggtgaat cgggcggcga gcgcggcggc
     6361 ctgtccagcg agaccctgct gctcgaggac ctggccgagg tggtcgatga cacccgccag
     6421 cggtcgctgc gcctgccgcg cgcggacggt gcggcgccgg cggacgctcc cctgccgctg
     6481 atcctgctgg gccacagcct gggcggtctg gtggcgggcc gttttgtgtc gctcgggctg
     6541 cggccggtcg atggcctggt gatgtcctcg ccggcgctgg atcccgggct caatgcgatc
     6601 cagaaacttt tgctggccac cctgccggcg attgcaccca atctgcgcgt caacaacggc
     6661 ctcaagcccg agttcatttc gcatgacccg caggtggtca gcaagtacct ggccgatccg
     6721 ctggtgcatg ccaagatttc cgcgcggctg gccaagttca tcgcgaccgc cgggccggca
     6781 acggtggccg ccgcatccac ctggcgcacg ccgacgctgc tgatgtatgc cggtgccgac
     6841 aggctggtca acccggccgg cagccgtgct tttgccgagg ctgccgcgag caacggcggc
     6901 gccaaggtcg tgaccacgcg ctgttttgac gagctgtacc acgagatctt caacgagctg
     6961 gacgcggcgc cggtgtttgc cagcctgaag gcctggctgg acgcccggtt ttaagccaaa
     7021 taagcctgca gccctcgccc ttcctgcgca gacagctcct gaatttatag cgaatcaggc
     7081 gctgcgcaag gcccgattcc ggagtgcgag ccaggccagc gcaaaccccg tcaggcccag
     7141 cggcagcagc gagcccaggt tcagcagcgt ccagccctgc gtggtgacca gcacgccgga
     7201 ggccagtgag gacagcgcca gcaccgcgaa cacgcagaaa ttgagcgcgc cctgcgcctt
     7261 gtctttttct tccggccggt aggcggtcag ggccagcgtg gtgctgccgg tgaacaggaa
     7321 gttccacccc accccgagca aaaacagcgc gatgaggaac tggtggagat cgacccccga
     7381 cagtgccacc agcacacagg ccacattgag cgccaggccc accgccatga tgggcagcgt
     7441 gccgaagcgc ttgatcaggt ggccggtgaa aaaaccgggc gcgaacatgc cgatcacatg
     7501 ccactcgagc accagcgcag cgtcggaaaa cggcaagccg cagacctgca tggcgatcgg
     7561 cgtagctgcc atcaggaggt tcatcacccc gaagcccagc gcgccggccg cggccgcgac
     7621 gatgaacacc ggctggcgca tgatctcgcc caggggccga ccgccgctgg cggcttgctg
     7681 gggcggcgtt ggcgggaagt ggatgaatgc cagcaaggcc atggccagca gggccacgcc
     7741 ggccagcgcc aggtaagccc cggcaaaagg aacttcggtc aggctgcgtg tgcccgctgc
     7801 gagattggga ccggcgatgg ccccgatcag gccgccggcc atcaccagcg agacggcctt
     7861 ttcgcggaag gccggcaacg ccagctcggc ggcggcaaaa cggtagagct gcgcattggc
     7921 gttgtaataa ccggccacca cggtggcggt caccagcagc cagaagttcc ggctgtaggc
     7981 cgcataacag cacagcagcg ccgacaggac cgccacccct agcccgaact ggaacgaggc
     8041 cttgcggccg aaacgcttct gcgtgcgcgc gacgatgccg gttgacagtg ccccgcccac
     8101 cacataaccc atcaccggca gagtagccat ccagcccagc ggtgccagtg ccagcccgac
     8161 caggccgttg atggcgatga aggtgacgtt gttggtgaga aacaggccct ggcagacagc
     8221 cagcagccag agattgcggt tcatcgttca gttttccagt gtaagcaacg ccaggcaggc
     8281 cagcggcgcg gtttcggcgc gcagaacgcg cgggcccagg gtcaccggca gaaagccgcg
     8341 ggccagcgcg gtgtcttctt cggccgtgct caggccgcct tcggggccgc tgagcagggt
     8401 cacgggggtc gtgctgtcat acgcagccag cgcttgcgcc aggggctgcg tgccggcgcg
     8461 cagcgacagc agcagccgcg gttcggccga cgcgacgggc ggcagggcgg ccagccagtc
     8521 gctcagtgtg tcgaccgcat gcacaggcgg caggcggttg cgcccgcact gttcgcaggc
     8581 ggcgatggcg acgccctgcc agtgcgcgac cttcttgtcg gcgcgttcac ccttgaggcg
     8641 cagcaccgaa cgttcggcca tcagcggcgt gatgctggct gcgcccagtt cgctggcttt
     8701 ttcgaccagc cagtccatgc gttcattggc cggcatgccc acggccaggt gcacggcgcg
     8761 gcgggtttca cgctccacgt cgctgtgggt gtcgaccttc acctgcacgt cgctgcggcc
     8821 catgcgttca atcgtcgcct ggaactcgcc gccctggctg ttgaacagcg tgatcacgtc
     8881 gccgggctgc aggcgcagta cctgcacgtg gcgcgcggcg cgctcgggca gggccagcgc
     8941 atcgccgctg gccagcggca ggtcggcata gaagcgggct gtcacggccg gtcctcgggt
     9001 gcttgcatgt tgctatgaaa tatgtagctg tccgtgcttg gtcccaaagg gaccgggccg
     9061 gattcgacgt tggaga
//
LOCUS       DAIDFG010000107         9024 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_107_length_9024_cov_15.711339, whole genome shotgun sequence.
ACCESSION   DAIDFG010000107 DAIDFG010000000
VERSION     DAIDFG010000107.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 9024)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9024)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9024
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107_length_9024_cov_15.711339"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1675 a         2805 c         2875 g         1669 t
ORIGIN      
        1 ggtgcaacgg ggtgaactcc gccgcccagc accacaatcg cctgcacgcc gctgcttttc
       61 agcgcggcga ccgtcaccgg cgcatgctgc ggcaggaggg tctgcgccag ccacaccgcg
      121 accgcgtggc aactcaggaa ccagagcagg gccacaccgg caaaggccag cagcaggcca
      181 ggcgcctttt tgcggatcgc cagcaacagg cccagcaggg cgagcagcag cggtgccgcg
      241 ggcggcagca ccagcgtggc aagaatcggc ttgagttcac ccagtgacat cattcaaaat
      301 gctctcaaat aaatagctgc ttgcgcaggt acagaaaggc ctggaggcca atttgactca
      361 tgaacttcag gaaacaggtt gtcgggcgac ggcaggacgg tcaaggggtt tttctgtttg
      421 ccggatgatc gacgcctgaa ttcaatacca ttcggtcggg gccgcgaaaa caagccggaa
      481 ccgaccgatc gtaaccaaac aaggaccacc acccatgaca caagacccat cgaacagcac
      541 cagcccgcag accatcacgc tgggcggcgg ctgcttctgg tgcaccgaag cggtgtttgt
      601 gcaggtgcgc ggcatcgtgg acgtcgagtc gggctacagc aacggccatg tcaacaaccc
      661 gacttacgag caggtgtgta ccggcaccac cgggcacaac gaagtcgtca agctggtcta
      721 tgacccggca caaatcggcc tgcgcgccat tcttgaaatt ttcttcgtga tccacgaccc
      781 gaccacgctg aaccgccagg gcaacgacag cggcacgcaa taccgcagcg gcatttacta
      841 ctcgacgccc gggcagaagc aggtggccga ggacatggtc catgagctga acgcgcaaaa
      901 gctgttcggc aagccggtgg tcaccgaggt attgccggtc agcaattact ccgcggccga
      961 ggagtaccac caggacttct tcgagaaaaa tcccaaccag ggttattgca tggcggtcgc
     1021 cggtcccaag gtggccaagt tccgcaagac gttttcggaa tacgccaaga cctgagcgtc
     1081 ccgtgccgag cacatcctcc gccccgacgt catgaccatc cccgccgaat accagcgcgc
     1141 ctccgtcgag ttcgagaagt tcatggtcga cgcgcgtgac ctggccgggc tgtccaccac
     1201 gaacatggct tacaacatgg tggtcggcgt gctgcatgtg tttcgtcgcc ggctcagcgt
     1261 gccggaggcg cttcggtttg caggtgttct tccggcagtg ctgcgcgcta tttttgtgtc
     1321 cgactgggac accgaacagc cccggctggc cttcagcgat cgcgcgtcca tgaccgggga
     1381 agtgaagcaa ctgcgcaagg cgcacaactt ctcgcccgac acggccatcc acgacgtggc
     1441 cctggccctg cgccgccatg tggacccgca ggaactggag catgtgcttg catcgctccc
     1501 gccggaagcg caggcatatt ggcgtgttta gctgagcttg tgttgcacag ctgcctgccc
     1561 ggcgaagagt ctgtgatgct ccctacaggg tgtggatgct cctgatgtga tagctgatgg
     1621 tgcccgttat tattgggcta caggcatttt ttgcttgaaa tcagccccgg cgccgcgcca
     1681 tgtaaaacca ctcctgcccc ggctcggcgc gtgggttggg aaacaccaca aaaccgtcgt
     1741 gcggcgcttt tacctcggcg ccgctttggc gcataccgat cagctcgccg gcctgcaccg
     1801 catcaaagct gcgccactcg cggctgaagc tgtcgcccgc gtgttcgcgg tccgtcacat
     1861 cgaccaggcg gaggatttcg cgctcggcag ttgaggcttc catcggctgc gacaccatgc
     1921 ccagcagcgc cagcgtctgc aagatcgcat ggcgcgccac ctgcacgccc tgcggggcgt
     1981 catgctggcc gcattccagc gtcacgccgt agccgccgcg tgaacgcatg tattcggtgg
     2041 tgccgacgcc gtagttcggg ttgatcgcca gcgtcagcgc gcgcgccgca ccagcaacag
     2101 gaggcgccgc agcgcggcgc gacacaccac gcgcgtaggt gtccagccag ccttccacga
     2161 tgcgcggggc gccggtgcac agggcgagct gcatttctgc ttcggcctgg ctgaaaggtt
     2221 ccagcgtgcc catgttgttc tgcgggccga tcatcacaaa cggtgcgccg ccggtgtgga
     2281 aggagtgcag gtccagcagc acgtcatgcg cgtccagcag tgggcacaac acattcgcta
     2341 tgcggtcttc aaaatcctgc ggaatcgcgc tcggcgccat gttgcggttc aggttgcgct
     2401 cgccggtgcg ctgcttgagc tggtaggcca gcgggttggt gacgggcaca aaggtgaccg
     2461 tgccgcgttc aatgctgagc tggccgctgt cgatgtcctg cagcaactgc gcggtggctt
     2521 tcgcgccgca gacctcgttg ccgtgaacgg cgcccaggac cagcagcttg gggccggggg
     2581 ccaggccgtg gaagctgtgg gtgcgcaggt gggtggggcg aggttcggaa acgacgggcg
     2641 tttgtttgtc ggtcatgagg attgttttgt gcggtacctg gactgggttt gaccggtgtt
     2701 gaggtttcgg tctctcctgc atttgcagga tgaagggttc cagtttcgcg cctatgatct
     2761 gcgatgtgcg gttttcattt tatcgactta actgggagcc gcaatgtgcc ggaactcctt
     2821 ggctgacata gacttaaaaa tcgacctgat gttctcgtta acccgcattc accaagctac
     2881 accattggtt tggtcgtgca catacatagt tagacctcgc ccaatttgcc catctacatg
     2941 agcaccaaca aaaccatcag agccgactgc agggcgtgct ctcgcagcac aaggcatgag
     3001 gtcctgtctc agcatgtgga cgagtccagt ccagacgttt accacgagaa ggacacttgg
     3061 caaatcattc gttgcctcgg atgccacaca tgcggcttca ggcaccgcaa cgatgactac
     3121 gaaatggtgg aggaagatga cgaagggagt tactcccatc aagtcacaac tcacttatat
     3181 ccaagcgtac tatctgggca tcgcccgctc tccgacacct actttcttcc acgactgatt
     3241 cagcgggtct ataagcagac attgagtgcg cttagccaac gagcctatgt attagcaagt
     3301 gttggacttc gtgcctgcat cgaggcggtg tgcaaccatc tcaaggtttc tggaacgaat
     3361 cttgaaaagc gcattgatca gctatacaaa gcagggcatg tgtccaacgg cgataagcgt
     3421 cgcctacatg ccattaggtt tttgggtaac gatgcggccc atgaaattaa ggaacctaag
     3481 gaatctgaca ttcgcgttgc acttgagatc gtggaacatt tattgaattc cgtcttcatt
     3541 cttgagaaaa aagcgaaggc gctcgacacc atcgctgaga gttttgacga cttcttgaag
     3601 attttgagta cctctgcaaa gacttttacg ggctcaactg cagttagtct ttcaggtctg
     3661 cttggaccga agcgtagact ggtgaatcaa aatattgatg acttcgagac aaaactcaag
     3721 caagagattg aggcaggatc ggtggccttc ttgaaactat cgcagtctcc attagtcgga
     3781 ggcaaagagg tacagttgta cgaggtagac tccgcgaagg ccgctgacgc cgacgacgac
     3841 atcccctttt agcgcacgac gatgaggctc ctgattccgc gcaccttggt tcgctgtgcg
     3901 cggcaacggc gccgttggcc ctagaacaac tttgagccgt acctgcacat gcccttgcgc
     3961 gacattcgta gagaattcgg cattcgggtt gccccatgcc gccgtgcctg ggacaccccc
     4021 tgatttttta cagtgttcgc ctggccagcc tccggggttt cggtgataat gcaaccaaat
     4081 tgcgttatgt atcaccactt gcagcgcatg ttgcccgata tcccatggcc actgtcctcc
     4141 accacaaaga agcagacccg atcgacgcct tgctgtcagc ccacggcctg cgccgcacga
     4201 ttgctgcgcg ccgcgtgctg gggtggctac tggcgcatcc ggacacgagt tacacgcatg
     4261 cccagttgca ggcggcgctg accgagggag aagaacggcc tggattgcgg atccagtccg
     4321 caatgacaaa gacgggaaag aaggcgaagg cggcggaaat tgcgccgctg gaccgcgtca
     4381 cgctctaccg cctgatcgac cgcctcacac aagtcggcct gctgctttgc cgggtggatg
     4441 cgagccgggt gcgccgctac caggcgatgc cggcgggtgt gcatgcgatg ccgcactttg
     4501 aatgccagag ctgccaccgc gacagcccgc tggagaacgc cttgcaggcc agcgccggcg
     4561 acctcgaccg tgcggcgcaa tcggcgctcg aagccctgaa ggcgctgggt taccagggca
     4621 tgagcctgga ctttgcggtg cggggcgtgt gcgccgattg cgccaccagc gccaagggcc
     4681 tgccagcgtg atccgtacgc ggcaactggc ctaccagtac cccggcgggc cggcgcttcg
     4741 ctttgacgac atcgatgttg tgcagggcgg ggtgcttctg ctgcgcggcg catcgggttc
     4801 gggcaagtcc acgtggctgg cactcgcggc tgggctgctg acgccgaccg ctggcgacat
     4861 cacggtagcc gggcagtcgc tgaccgcgct gggcaaagtg gcgagtgatg cctggcgcgc
     4921 gaagacgatt ggttttctgc cgcagaaact tcatttgagc agcgcgctga cggtgcacgc
     4981 caacctggcg atcgcgcagt gggccgccgg tcaggcgcag gacgatggac gcatccgcga
     5041 ggcgctcacg gcgctcggtg tgaatgagct cgccatgcgc aagccggcgc agctctcggg
     5101 cgggcaggcg cagcgtgtgg cgctggcgcg ggcggtgttg ttgcagccgc aggtgatctt
     5161 ggcagacgaa ccgacggcca gtctggacga cgacgcggcg cggcaagctg tggaattgct
     5221 gcagaccacg gcgaggcagc agggcgccac gctggtgatt gcaacgcatg attcgcgtgt
     5281 ttcagccagt cttgatagtc aaatcggctt ccagcccttg tacctcgggc gcgagcagct
     5341 atgaatacca tagcatttgc ctggcgtttc ctctggtccc gaccgcttgc ggcggggctc
     5401 aacgtgctgc tgctcagcct cgggctggcg tcgctgagct ttgtgctgtt ggtcagccac
     5461 cagatcgacc aggcgtttga acgcgacctg gccggcatcg acgtggtggt gggcgccaag
     5521 ggcagcccga tgcagctgat cctctcgggc gtgttccaca tcgacgcgcc caccggcaac
     5581 gtgccgctgg cggcggtgaa ggaactcgaa caacacccgc atgtggcgaa gctgatcccg
     5641 ctcagcctgg gcgacagctt gcgcggcttc cgcattgtgg gtaccacgcc ggactacctc
     5701 acccattacc aggcgacgtt tgcgcagggc agcgtctgga cggcgcccat gcaggcggtg
     5761 ttgggcgcgc aggtggccaa gcaaaccggc ctgaaggtgg gcgaccgctt cgccggcagc
     5821 cacggcctgg ccggcggcgg tgacagccat ggacagacgc cctacgtcgt cagcggcgtg
     5881 ctggcgcccg gcggctcggt gctggaccgg ctggtgctga cggcgctgga ttccgtctgg
     5941 caggtccatg aagatcacac ggccctcgac gccgacgacc gccaggtgct ggaagacgag
     6001 cgcgaaatca cgctggcgct gattcagtac cgcacgccgc tggcggcggt gacctttccg
     6061 cgctttgtga acagcagcac cgagatgcag gcggccgcgc cggcgctgga gatcacgcgt
     6121 ctgctgggca tgatcggcgt gggcaccgac gtgttgcgcg cgctggctgg cgtgttgctg
     6181 ctgacggcgg gcctgagcgt gttcatcgcg ctgtggagcg ccgtgcgcga gcgccgcgcc
     6241 gacctggcgc tgctgcgttt gctgggcgcg ccgccgcgca aggtggcggc gctgttgctg
     6301 tgcgaggcgc tgtggctggc gctgctggcg accgtgatcg gcgtgctggc ggggcagggg
     6361 ctgatggcgc tgctcgcctg gctgctgcag ctggacaagt cggtcctggt cggtgcgttg
     6421 tcgtggccgg tcgggttggt gagtgtgccg gttctggccg tcggtgtggc gctgctgtca
     6481 gcgctgctgc ccgcgtacga ggcctaccgg gtccctgtgt ttgaactttt acagtcgagg
     6541 tgaaccccat gatcaagaaa tatatgaaaa aaatgcttcc tgcccttgcc acgcttgcgc
     6601 tgacagctac gttttcaata gcgcatgcgc aacacaagga cgcagaaacc aaggaagaca
     6661 tccagcgcca ccgcgccatg gcggccgcgc atgaggccgc ggccaaatgc ctggagtcgg
     6721 gcaagaagga agatgtctgc atcaaagagt tgcaggcttc ctgcaagggg ctggcggtgg
     6781 gaaaatactg cggcatgaaa cacgcgcatt gacgcgccgc cactgcaacg tttctggaga
     6841 acccatgagc ccctctgctt ccaccacttt caagacggca ccgctgttgc tggccctgct
     6901 gttcgccgca ggcgccgcac gcgcgcagct gagctcgccc ctgcctgccg gcagcggcaa
     6961 gcccgcggcg gccaaggcgg cgcctgccgc accggacggc ctggcgctgc cggccgggca
     7021 gggcgcgggt gtgcacgggg ccaacagccc gtatgcgccg ctggccccgc gcggcgacgt
     7081 ggtgccctgg tcggtgctga ccgacgtgaa aaccaaaaac gagaagaacc gcatcctgcc
     7141 ggtgttcaat gaaagccagc tcggcatgaa cggcaagacc cagcgcatcc agggcttcat
     7201 gatgccgctg gagccgggtg aaaagcagcg ccacttcctg ctgtcgtcgg tgccgctgac
     7261 ctgctccttc tgcgtgccgg gcgggccgga aagcatggtc gaggtcaaga ccaaaacccc
     7321 ggtgaaatac agcatggagg tcgtggtggt ggaaggccgc ctgcaggtgc tgcacgacga
     7381 ctcctatggc ctgtattacc gcatcaacga tgcggtgacg gtgaagtaaa cacccatggt
     7441 tttcaggcgc atggtgtggc gggcagcctg gggctatctg gtcctggtgc tcctgcttgc
     7501 gccggtgctc ggccagatgc acggcctggt tcacggcctg gttcacggcg ctggtggcga
     7561 ccgtccggct gcgcaacaac tccttgaggt cgatgctcat gcgcacgacc acgcgcatga
     7621 acgcgcgggt ggacatggct ggctcgcgga tcttttctcc gtccatcttg acgattcgga
     7681 ttgccgcgtt ttcgatcagc tgtgccacag cgatgtgctg ccggctgcgc cgcccttggc
     7741 gctgcccatg gtgctttccc cgtttgtttt tcaatttctg gaaggcgaag tactcgcccg
     7801 ccgggccgcg cttttcgaag cacgcggccc accccgggct cgttgaactc ccgaagttgt
     7861 cgagcgcggc cgcatggtcg ttgcttgacg ttttctgttt ccgattcaac gagccctcat
     7921 gaaaaaacca ttcccccgcc ctgccatcgg cacggccacg ttcgccctgt tcgcgctcgg
     7981 ctgctcgctg gcccacgccc agacagcggc gcccacactg aaagaaatca ccgtcaccgg
     8041 caacccgctg ggtgcgacgg acctgatcac ccccgccacg cagtattcag gcgccggcct
     8101 gctgttgcgc tccaaaacca cgctcggtga aacgctggat ggcatgcctg gcgtcagcag
     8161 cacctacttc ggccccaacg ccagccggcc cgtgattcgc gggctggacg gcgaccgcat
     8221 ccgcatcctg gccaacggtg gcggcgcgct cgatgcctcc agcctgagtt atgaccacgc
     8281 ggtcacggcc gatcccatca gcatcgaacg catcgaggtg ctgcgcggcc ccggcgccct
     8341 gctgtacggc ggcagcgccg tcggcggcgt ggtcaacgtg atcgacaacc gcatcccgcg
     8401 cgagccgcag ttcgacgaga agggtgggat cagcggcaag gccgacctga gcctggccag
     8461 cggcaactcg gagcgcggca aggctgtgct gctcgaaggc ggcaacaagc gctacagcct
     8521 gcacgtcgat gccttcgacc gcgagaccag cgacgcgcgc gtaccgcgcg acctgaactg
     8581 caccaaggcc ggggtgacca ccagctcccg ccgcatctgc aactcggcca gcgacgtgca
     8641 tggcggtgcc gtcggcggct cggtgttttt cgacaacggc tacctcggcg cgtcggccag
     8701 caattaccgc agcacctacg gcacggtggc ggaagacgaa gtcaccctcg gcatgaagtc
     8761 ggaccgttat gcgatcgaag gcgaagtgcg caacctgggc gggcccatcc agagcatcaa
     8821 gggccagtac agccagactg actacggcca caccgagttc gaaggcgcga ccgccggaac
     8881 ggtgttcaag aacaagggca gcgatttccg gctggaagcg cgtcacgcga aactcggcaa
     8941 gctcgacggc gtgctgggtt tccagaccga gtccaaccgt ttttccgccg acggcacgga
     9001 agcctttgcc ccctacagca agac
//
LOCUS       DAIDFG010000108         9006 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_108_length_9006_cov_18.899117, whole genome shotgun sequence.
ACCESSION   DAIDFG010000108 DAIDFG010000000
VERSION     DAIDFG010000108.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 9006)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9006)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9006
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108_length_9006_cov_18.899117"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1721 a         2777 c         2979 g         1529 t
ORIGIN      
        1 cctggatctt ccatgtgcgt gccggctatg ccgacgagac agaacacatg gtgcagcagg
       61 tgaccacgct gggcatgagc cgcatcgccg tgatgtacca ggatgacggc ttcggcaagg
      121 ccggcctgct gggcgtcgaa aacgcgctgg ccaaacgcaa gctgaagctg gtcgtggcgg
      181 ccggttatga gcgcaacacc gacaaggtcg aagaggcggt caagaagatc aaggcgtcgg
      241 acgcgcaggc catcatcatg atttcggtca acaagtccac ggcggccttt gtcaagcttt
      301 accgcgagac tggcggcggc gcgcagctct acaacatctc ggtggtggat ccggccgaac
      361 tcgtcaggct cgccggcctg aaaaacgccc acggactggg catcagccag gtggtgcctt
      421 accccttcat gccgaacacg ccggtggtgc gcgaatacca tgcgctgctg aaaaaatacg
      481 cgcccggcga agtcgtcaac tacacgagtt ttgaagaatt tgtcggcgcc aaggtgctgg
      541 tcgaagccct gcggcgcgcc ggccccaacc ccacgcgcgc cagggtggtc aaggcgctgg
      601 agtccatgaa cagcttcgac accggcggtg tcatcgtcaa ctattcacct gccaaccgca
      661 tcggctcacg ttatgtggaa gtgaccgtga tcggcagctc cggcaagctg ctgaagtagc
      721 cactgcggcg cgcgcccaga gacgaacaga ttaaacaaga gagtccgctt catgcaattc
      781 acgtctgagt atttgacctg cgccgggcgc gagatccact ataccgaatg gggagcgcag
      841 cacagcgcta ccgtcatcgc ctggcacggg ctggcccgca ccggacgcga catggacgaa
      901 ctggcggcgc acctgagcga ccgctaccgc gtgatctgcc ccgacaccat cggacgcggc
      961 ctgagtcagt ggagccccga cccgaaaggc gaataccagc tggcgtttta cgcgcgtatt
     1021 gcggctgatc tgtttgagca actgcacatc gacaaggcgc actgggtcgg cacctccatg
     1081 ggcggcgcca tcggcacggt ctgcgcgtcc ggcctgttcg agcccgccat gaaaggccgc
     1141 atccagagcc tggtgctcaa cgacacggcg ccgcagattg ccgatccagc cgtcgagcgg
     1201 atcaagtcct acgccggcaa cccgccggcc tttgacacca tgctggcgct cgaagctttc
     1261 ttccggcagg tgtacaagcc ctatggctgg ctcagcgacg tccagtggcg ccgcatgacc
     1321 gagacctcgg cgcgccgcct gcccgacggc cgcctgacac cgcactacga cccggccatg
     1381 gtgcagcagt tcacccacca cccgggcgac tacctgatct gggagcatta cgacgcgctc
     1441 aagattccgg tcctgtgcct gcgtggcgcg gagtccgacc tcgtgctgcg cgacaccacc
     1501 gccgagatgc tgacgcgggg gccgggtgcg gccggcttga cgcaagtggt ggagattgag
     1561 ggctgcggcc atgcgccggc gctcaacgtg cccgatcagc tggagcgggt ggcggcattc
     1621 atcgacgcgg cgggctgagt tgctattgat tcgatagctg gttgcgcccg tggagaaagg
     1681 gctggagagc aattttatgc atcttcccgg cgccaacgcg ctggcgcgga tcacgtttca
     1741 tcgagatagc cgcgctccgt ggccaaggcc tggttttcaa aaatcgccat ggctgtgcgc
     1801 atgcgcctga accccagttt tcggtagaag ccttccttgc cgggaaccgc gtagaggatg
     1861 attttccggt ggccacggga caggtccacg agtttgccga cgatctcttt acccaggccg
     1921 gtgccctgat gactcggcaa gacagcgatg tcgcagatgt aggcgcagtc cacgccatcg
     1981 gccagcgccc ggccaacccc gaccagcttg ccgtcctcgc gaacgaagca ccggaacatg
     2041 ctgttcgtga agacggtttt caggtcggcc ggctttttgt tccccagcgg cgccgcgagg
     2101 taaagggcgg agagttcgtt ccagtcgatg ttgtcgacga ggtgggtcca gaccagcggc
     2161 acattgttct cctgttcaat caagggtcgc catcttaggg ggcccagtgg taggtggccg
     2221 ccgcatgcag cgtccagctg gcgttaccgg gcagctccag cacctggtgg tggtgcttca
     2281 aaagtgcctg gtggtggctg acgctgacag gggtgatcga caggccggcg agctggccgt
     2341 acaggcgctc ctcgttggtc gcgtccagcg cgctggtggc ctcgtcgagc agcaggtagc
     2401 gcggcttggc gagcagggcg cgggcgaagg ccagccgctg ttgctcgccg acggacagca
     2461 ccttggccca gtccagctcg gcgtccaggc cgccgaaacg ttccgccagc gtcggcaggt
     2521 tcacccggtc cagccatgac agcaactctt ggtccgagac ttcgcgatcg gtctgggggt
     2581 aggtcagctg gcagcgcaga tcgcccagcg gcaggtaggg ctgctggggc aggaacaaca
     2641 tgtccggccc ggccgggcgc accacctcgc ccgagcccgt gttccacaag ccggccatca
     2701 cccgcagcag cgagcttttg cctgcgccgc tggcgccgac gatcagcagc ccttcgcccg
     2761 gggggaccgc cagcgtgagc ccctgaacca gcaggtgctc ccggttgggc gtgagcaccg
     2821 tcaggcggtc cagggccagc gcaaagccgg gtgtggtgct gatctgtggc ggggcgtcat
     2881 cgccgggccc ggggggctgg ttggcctggg cgtccagcgc ctgcgagaag gcatgcagcc
     2941 ggtccacccc ggccgagaag cggctcaggc cctcgaagtg ttcgacgatc accgtcagcg
     3001 cactcaggat ggcggcgaag gcgccggcgg cctggatagc ccggccgacc tccagcttcc
     3061 cggagagcac atcgcctgca atgatcacgg taggcagcac caccgtcaga aagctgtggg
     3121 cgaactggaa gaggttgagc ttgaattgcc agcgaatcac ctgctggaag ttggcataag
     3181 ccgcctggaa gacacgctgc agcgccgaca tctcgcgcgc ttctccgtcg taaaaggcga
     3241 tcggctctgc atgttcgcgc acccgcacca ggctgaaacg gaaatctgcc tcgcgctgca
     3301 gctggcggaa attcaggccg atcagccgct tgccgaacac cgagccggtg aaccaggtgc
     3361 tgaacacggc ataaccgatc aggaaataaa ccagcccttg ggaaatggac cacaacacac
     3421 cggaaaaggc gatcagctgg atgaaggagc ccagcacgat catcgagaag tacagcgact
     3481 gctgggtgaa ggtgttgatg tcctcggcaa tgcgctggtc ggggttgtcg atgccggcga
     3541 cggcgttgag ccggtagtag gcgcgctgat gaaaatagcg cgccaggaag tggtgtgtca
     3601 gccagcggcg ccagcgcagg cccagggtgt cgcgcacgaa gtagtacagg ccatagagcg
     3661 gaaccgccgc gacgaggatg gccgtgtagc gccagatcga ggcccagaag cgttcggtgt
     3721 ctccggcggc cagggccgag gtgaactcgc cggtctcgtg gttgaacagc acgctgacgg
     3781 ccgtctgcgc cagcaacagc aaaatcagca gggccagcaa cccccgggaa cgccagcgtt
     3841 cattcgcgag ccagtagggc cgggcgatcg cgacgaagcg tcgccacagg cgggcattga
     3901 agccgggtgc tgcttccggg gtcatgggtg caaggcctgc gggccgatgt cggaggggag
     3961 gtccattccc ctattggaca tgaattcagg gcggggtcag cacggcggtg atcacggcct
     4021 cgtcgggcgc cgcgaaggtg gtgttgtgcc gctcggtcag ggggccgccg ccgggaaaaa
     4081 cttcaccctg ggcgccgtaa ccaatggcct tgaacttcca ctgcccccca atcagcttga
     4141 ggttgcccac gtggaggtcc ggaccggcga gcacgcggta cacgccgtca ttgaccctgc
     4201 tgagcgtcag ggctggcaag gccttcatct gcgcttgccc ggacatgccc ggccaccggc
     4261 catcgtcagg ccctgatggc cgcgaagagc tcgcgcttgt cgaggccgta catgtcggca
     4321 aattcctcgg cggtcttgcc actgctttca aacgtgcggc gcagtgcttc gcgtttggcc
     4381 agcttctggt cgcgctcggc aaaggcttcc tcgagcaggg cggtggcctc caggtcattg
     4441 gtctggaccc gggcctggta gtcctggcgg gtcaggccgc tggcctcgaa agcggccatc
     4501 aactgtgcac ggaatttggg ccgcaggtcc tcgcgggcgc gggcctggcg gcggcggaac
     4561 agttcaagca gtgacaggta gacatgttcg ggcgccacgt cgtcacccgc attgccctgc
     4621 aggtcgtggc gcttgttgcc ggcggtcacg cattgcaggt aacgcgtgga gcgtgtgtgg
     4681 atgcccagcg ccgccttgag gctgtcgcgc tggaagtgtt cggggtgcgc ggccagcagt
     4741 tcctgaaaaa tgccgagctt gagcggcagg aaattggcgc cgaagaggtg cgggtacagc
     4801 tcgaacagct tttccagcac gggctgcacc gaacgcgaac gacccgctgc aggggtgggc
     4861 tcgcctgccg gcgcgctgga gggctgcccg gccggcgtga tctcgtccgc caggacggtt
     4921 tcaccggggg caacgcttgt ttctgaaggg gtcatggtca tgggggtcat cttggggtca
     4981 acccgtgatt gtcaaccaaa cggcccgccc gctgtgtaaa ccccatgttc atgggggctg
     5041 aattgtcgtc agcctctggt ttttggctgc tccgggcatc cataatttga cctgtcatgc
     5101 gggaagggat cgccgcatgc caggcctgcc gcgcacgccg gtttgtttgc gctttgtgtt
     5161 caaaatattt ctgacaggaa acaaccatga cttctggaat caaggctgtc gcagccagac
     5221 tcgcttttgc cttgctgctt tcatttgccg gggcccaggc catggccgcg ggcgcgctgg
     5281 ccattgacag cctgcagggt gaaaaatacg gattctcctt caaccacccg acgtccgacc
     5341 aggccgagaa gcgtgcgatg agggagtgtg gttcagactg cgccgtggtg ctgcgcttcc
     5401 gcgccgaatg cggcgcctac gccgcagacc aggccaaggg ctccaatgcc tacggttggg
     5461 gaacgggtgc gacgagttcc atcgtccagc agcgcgcatt gtcggaatgc cgggccaagg
     5521 gcggctcaag ctgcaaggtc cgttcatggg gctgtgacgc cacgaaggcc tcggccgacc
     5581 aggattccgg gtccagggac aacaacgcgg ccagcgcgga attccagatg cagggtgact
     5641 gggaggtcga ctatgccgga tcagccggat ttccttaccg gggaaccttg cgcgtcaacg
     5701 aaaagtccgg caacggcgtc tatcggggcg tgatggttct ggcttacacc aacaatcagg
     5761 gccaatccaa gaaggttcag cagaacacgc tgatcacggt tcgcggaaac atcgtcacga
     5821 tcaagggcag caatcctgtc tacctgcagg gttcgggcaa ctacgatccc gacacttaca
     5881 ccatgaccat aggcagttcc aatgtcatgc gcggccagaa caacgatgcc gggggcgctg
     5941 gcggttccgt ggtgatcacc cggcgataaa cccctccggg gggcggtcgg gtgtcccgct
     6001 tgggccagcg cctgcacatc gggcccgacc taaaatccgg gtgaatgaaa caccgcagag
     6061 ttccctcacc gcagcccggg cctgcgcttg cgcccatggt ggcccagccc ctgggcgtga
     6121 agctgcaagc ggctgcggcc ctgcatcagc aggggcagct cgatcaggcg gaagtgctgt
     6181 acaaggaaat cctgcaggcg cagccgcggc actttgatgc actgcaattg ttggcaacgc
     6241 tggccgtgca acgaaaccat gcggcgcaag ccatggttct gttcgagctg gcactgaaga
     6301 tccatcccgc gaatgccagc gtgctcagca accgggggtc cgccttgctg aacctgaacc
     6361 ggccgcagga agcgctggac agctgtgatc gcgcgttgca ggtcaggcct gactatgcag
     6421 aggccttgag caaccgcggc aatgccttgt gggcgctgaa gcgcccggcg gaggcgctgc
     6481 agagtttcga ccatgcactg aaactcaagc cggactacgc tgaagcgctg agcaaccgcg
     6541 gtgcggcctt gcgggccctg aaccgccatg ccgaggcgct ggacagttat gaccgcgcgc
     6601 tccggatccg tccggccttt gcccaggcac tgaacaaccg gggcgccgcc ttgcaggacc
     6661 tcaaccgcca tgcggaggcg ctggaggatt ttggccgcgc cctgcaggtc cagcaggacc
     6721 atgcagaagc ccacgtgaac gaagcgctgt gccgactgat actgggcgac tttgcgcagg
     6781 gctggcaaaa ctacgaatgg cgctggaagc gaaagctcaa ccaggacaag cttcgcaact
     6841 tcagccaacc cctgtggctg ggcaaagagt cgttggcggg caagacgata cttctgcacg
     6901 ccgagcaagg cctgggcgac acccttcaat tctgccgcta cgccaggcag gtggcggccc
     6961 tgggcgccac agtggtgctc gaagtgcagc cagcgctcaa gacactgctt caagacctgg
     7021 aaggtgtcag cctggtgctg gggcgcggcg agcgtctgcc cgacgttgac taccactgcc
     7081 cgctgctgag cctgccgctg gcgttcaagg tcgatctggg caccatcagc ggcgagccct
     7141 acctgcacag cgacccggac aaacgccagg cctggcaaga gcgactgggc cctggccgca
     7201 acaagcgcat cggcctggtg tggagcggca gcaccggcca ccagaacgac cacacccgat
     7261 ccctggcgct gacggacatc cagggactgc tcagcgagca ggccgattgc tactgcctgc
     7321 aaaaagagct gcgcccggcc gaccgggagg cgctggcaaa cacgcccgga ctgaccttcc
     7381 tgggcgattc gtttcaggac ttcagcgaca cggccgcagt ggtcgccctg atggacctgg
     7441 tgatcacggt ggacaccagc gtggcgcatc tggccggggc gatgggcaaa gaagtgtggc
     7501 tgctgctggc ctacagcccg gactggcgct ggttgcttga acgcagcgac agcccgtggt
     7561 acggctcagt gcggctgttc aggcagagtg ccccgggcga ctgggccggt gtgttgactc
     7621 aggtgtgggc ggcgttgaag ctgcgcttgg gtcgacacaa agacaacgcg gctgcagaca
     7681 cgcgtggcgt ggcgggcaag ctgaccagcg ctttggccct gcatcaaaaa ggcgatctga
     7741 ccaaggctga ggttttgtac aaggagattc tgactgcgca gccccggcac tttgatgcgc
     7801 tgcagatgct ggcgaccatt gctgcgcatc gcaaggacaa cgctgatgca gttgttttgt
     7861 ttgagcaggc gctcaggacc cggcctgacc atgtgggcgt actcaatgat ttgggaaatg
     7921 ctttgcaagc ccagggaaaa atggaggagg cgcttcagag ttacggtcgc gcgttggaca
     7981 ttcagccgga ctttgtggat gcccttgtca atcgtggcaa tgcctggcga aggcttcaac
     8041 gtgttgaaga cgcgctggca agttatgagc gcgcactgca ggtcagtccc gaccatgcag
     8101 aagttttgaa caatcggggc gtggccttgc tggcgctggg gcgtccgcag gaagcgctgg
     8161 caagttatca aaaagcatgg cgggccaagc ccgactatgc cgaagcgcac tggaacgaag
     8221 cgctgtgcca actgctgctg ggtgacttta cgcagggctg gaagaactac gaatggcgct
     8281 ggaagcgaaa gctcaaccag gacaagcttc gcaacttctc ccaacccctg tggctgggca
     8341 aagagccgct gcagggcaag acgatactgc tgcacgccga acaaggcttg ggcgacaccc
     8401 ttcagttctg ccgctacgcc aaacaggtgg cggcgctggg cgccagagtg gtgctcgaag
     8461 tgcagcccgc actcaagaca ctgcttcaag gccttgaagg tgtcagcctg gtgctggggc
     8521 ggggagaacg cctgcccgac tttgactacc actgcccact gctaagcctg ccgctggcgt
     8581 tcaaggtcga tctgggcaac atcagcggcg cgccctacct gcacagcgag cccaaaaaac
     8641 gagcccaatg gcaagaacga ctgggcgcca gcacccaacc ccgcatcggc ctggtatgga
     8701 gcggtagcac cggccaccag aacgaccaca accgatcctt gggtcttcag cagatcaccc
     8761 ccctgatcag cgacgatgcc cagtactact gcctgcaaaa agagctgcgc ccggacgacc
     8821 gggcggcact tgcaaacaca cccgggctga ccttcctggg tgattcgttc gaagacttca
     8881 gcgacacggc cgcggtggtg gagttgatgg acctggtgat cacggtggac accagcgtgg
     8941 cgcatctggc aggggcgatg ggcaaagaag tgtggctgct gctggcctac agcccggact
     9001 ggcgct
//
LOCUS       DAIDFG010000109         8882 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_109_length_8882_cov_19.059250, whole genome shotgun sequence.
ACCESSION   DAIDFG010000109 DAIDFG010000000
VERSION     DAIDFG010000109.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 8882)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8882)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8882
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109_length_8882_cov_19.059250"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1758 a         2810 c         2689 g         1625 t
ORIGIN      
        1 cccggcccat catttttgcg ctggccaacc ccaaccccga aatcacaccc gaagacgtca
       61 agtcagtgcg tgatgacgcc atcatggcca ccggccgcac cgactacccg aaccaggtca
      121 acaatgtcct gtgtttcccc tacatcttcc gcggcgcgct ggacgccggc gcctccacca
      181 tcacggtcga gatggaaatt gccgcggtgc atgccattgc cgagctggca caggccgagc
      241 agagcgaggt ggtggcagcg gcctacaccg gcgagcagct ggcgttcgga ccggaatacc
      301 tgattcccaa gccgtttgac ccgcgcctga tgatgaaaat cgcgccggcc gtggcgcagg
      361 cggcggccga tagcggtgtg gcgctgcgcc ccatcaccga catggacgcc tatcgggaga
      421 agctccagag ttttgtctac gcctcgggca cgaccatgaa gccgattttt gcggctgcca
      481 aaaaagccgt caaaaaacgc gtggcctatg ccgagggcga ggaagaacgc gtgctgcggg
      541 cttgccagat cgtcgtggac gaaggtttgg cccggccgac actgataggc cggcccgcca
      601 tcattgccca gcgcatcgaa aaattcggcc tgcggctgag ggaggagctc gattacgacg
      661 tggtcaacgt cgagcaggat caccgctacc gcgacttctg gcaaacctac catcgcatca
      721 tggagcgcca gggcgtgacc gtgcagatgg ccaagatcga gatgcgccgc cgcctgacgc
      781 tgattgcggg catgctgctg cacaaaggcg atgtcgacgg cctgatctgc ggcacctggg
      841 gcaccaccgc ccaccacctg aactacctgg accaagtgat cggcaagcgc ccgggcgtct
      901 gtacctacgc ctgcatgaac ggcctgatgc tgccggaccg ccaggtgttt ctggtcgaca
      961 cccatgtgaa ctacgacccc acggccgagc agctggccga gatcacgaca ctggctgcag
     1021 aggaaatgat gcgctttggc atcaagccca aggccgcgct gctgtcgcac tcgaattttg
     1081 gctcgtccaa tctacccagc gcactgaaaa tgcgccacac gctgcaattg ctgcgagacg
     1141 aagccccgtg gctggaagtc gacggcgaaa tgcacggtga tgtggcactg gacgctgcgg
     1201 cgcgggcggc ggtcatgccc aacagcaccc tgtccggtga agccaatttg ctggttctgc
     1261 ccaacatcga tgcagccaac attgcgtaca acctgctcaa gacggcagca ggcggcaaca
     1321 tcgccatcgg cccggtcctg ctcggtgccg ccaaacccat gcacatcctg accgccagca
     1381 ccacggtgcg ccggattgtc aacatgacag ccttgaccgt ggcagacgcc aacgcatccc
     1441 gataagcttt ttttagacag ctagcactaa cttataaccg cacccaacgt ggcgatagac
     1501 tgcctgaatt ttgggcaggc ccttgcgttt ttctgcggga tgcgtcaaac tctacctctt
     1561 gaaatttaag ggttaacccg gcaactgcta aacaattggc ggttggctct ggttcaagta
     1621 aaacggattt ttccatgttg cgaaaaaatg gagccgggtg gcggctagtc gcagcagtcg
     1681 ttctggctgc tttgagcacg gggttcagcc cgtccggcgt agaagccaaa gggccagtcc
     1741 cccaggccga agtcagcacg atttcagtcg cggccctgcc ggagcagggg cgccacatgg
     1801 tccgcttgat ttatcagggg ggcccgtaca ggtacgacaa ggacggttcg gtgtttggca
     1861 atcgggaaag aatacttccc gccaaaaatc gcggttacta ccgcgaatac accgtcagaa
     1921 ccccaggtga gcgcagtcgg ggcgcccggc gcatcgtttg tggtggttta gtacccaccg
     1981 cgcccgatgc ttgttattac accgatgatc actacgcgag ttttcgcaga atcgctcaat
     2041 aaggttcaac agtgatcgtc aactttgaat tattgactta agaaagagac gcggagatgg
     2101 aaacaccact tcgtactgtt agacccaata tcgtgcaatc catacgcgcc ttccgcgtgc
     2161 aggacttgca ggatgctgcc acccagcttg gccaccactt cctctacgcc aacctgggca
     2221 acgcccagag caagcaggac gtgctggaca tgatcgcggc acagtacaca ttcccggcgc
     2281 attttggcaa aaactttgat gcgctgtacg actgcatgac ggacctggtt cacaagtctg
     2341 gaccgcagcc cggcttcatt gtcctgctgg agcagatccc tgccaatgca aaatttgaca
     2401 aggaagcgcg cgaacagctg ctggatatct tcagggatgc ggcggactac tgggcggatc
     2461 gaaaaatccc gttcagatgt ttctattctt ttctgtagcc cgctccccag acattggcca
     2521 aggggatcgg gcgaatgagg cccagcaagc aaacagcgca gacggcgaag agccgatgcc
     2581 gaccgacaaa ctgctcgacg tgtcgccgct ggcgttgcgc atgagcagcc cgttcaacgc
     2641 gggttactgg ttggccgcag cctgatactt ccaaacatgc tgcaaaaaag cccgttgttc
     2701 aacgggcttt tttcatgccg aaccgccgtc tggtcggtcg ccaccggatt gactgcaaaa
     2761 cggctgcgag cgctcaatgg atgagtgcca ttagctatca attaaatagc aaaatgacgg
     2821 tccggccagg actcaggcaa ccgccccgtt ttccagcgcc tgggccagcg ccacatattc
     2881 agccaccggc acttcctcgg cgcggcgctg cacatcgaac ggcgggctgt ggggccgctg
     2941 ctccagccat tggcccaggg agtggcgcag cagcttgcgg cgctggctga aagccacacg
     3001 cacgacctcg ctcagcagct tgacatccac cttctccggc gcagcccggg ggaccatgcg
     3061 aaccaccgcg ctgtccacgc gcggcggcgg atcaaaactc tgcggtggca caaacagcac
     3121 gttctccatg gcataacgcc actgcagcat cacgctgagc cggccatagt cgctggtcgc
     3181 ggggcgggcc accatgcggt cgatcacttc tttttgcagc atgaaatgct ggtcttcaat
     3241 gacctccacc gcgtcgagca ggtggaacag gatgggcgtg gaaatgttgt aaggcaaatt
     3301 gccgaccaca cggatttttc cggaaggccc tgcgctttcg gaagcgagct ggacaaaatc
     3361 aaccttgaga acatcggcct ggaccacccg gagctgcgga tgagcccgca gttgctgcgc
     3421 caggtccctg tccagttcaa tgaccgtcag cttgcccagg cgctctgcca gaggctgggt
     3481 cagggccgcc aagccgggcc caatttcaac catcgcctga ccccccgcgg gtgcaatcgc
     3541 gtccacgatg gcgtcaatga tgctgcgatc agtcaggaaa tgctggccga aacgcttgcg
     3601 cgggatatgt ttcactgcgc cagaccacgc acgcgatctc cgcgtgagac ggaccccgaa
     3661 atgccagtcc aagccgcgga accggctttg ccgggccgca ggcggggcgc ccccccgggg
     3721 ggcagcgagc tacacgcagt gagcgagcgt gggggcgcca ttactgaggc ggatcccggt
     3781 attcgacata ggcgcggccg cgcacttcct gcgcccaggc gctgtaggcc tcttcaaact
     3841 ttttctcgcg cagcgaagca cgcacaattt cacgctgctc gcgcggggat agcttggtgt
     3901 tgcgccgctc cagcacctgg atcaggtgaa cgccgaaacg cgacacgacg gggtccccga
     3961 cctggccggg cgccaggctg ttcatgcgct cctcgaactc gggcacaaac tggccggggc
     4021 cggcccagcc gagatcaccg ccgcccttgg cagaggcgtc ctggctgttc tcgcgggcca
     4081 gcgcggcaaa atcggcctgt ccggcagcaa tgcgcttctg gaaatcggcc agccgcgcga
     4141 gggcggccgc ctcgccaagc tgggccgtgg ggcgcagcag gatatgacga gcgcgggttt
     4201 ccaccaccgt ggcacccggc atgccggcgc gttttcgctc cagcaccttg agcacgtgaa
     4261 aaccggcacc gctgcgcacc ggaccggcaa ttccgccgat cggtacattt ttcgtggtgt
     4321 cgataaaaag ctccgggtag cggtcggcag ggcgcaagcc catctggccg tcggcgcttc
     4381 gccccggcgc attggaaaac tcgcccacca gcgcggtgaa gtccgtcccg ctgcgcgcgc
     4441 gttctgccgc cagttgtgcc ctggcctgga ggctggccac ctgggccgga gtggcgtttt
     4501 cgggaacgac caccaggatc tgcgcgaggt tgagctcctg cgaggcgggg tcgcccccgg
     4561 cttcctgctc gcggatgaac tgctccacct cctggtcgct gaccttcgcc tgggactcga
     4621 gttcacgctc ccggatccgg gtcagcagga tttcattgcg aagatcggtg cggaactggg
     4681 aaaagaccaa cccctgggca gtcacgcgct ggcgcaactc gtcaacggac aactggtttt
     4741 gccgggcgac attgagcacc gcctcatcca ccatggcctc gctgacctgc acccccagtt
     4801 ccctggccag ctgcagttgt gcacgctcca cgatgatgcg ttcgagcagc agccgtgcca
     4861 gctcgttttt gggcggcaac gccgtgcctt gctgcgccat ttgctgctcg gtgcgcagca
     4921 tgcgcgcgcg cacctcgttg ttggtaatcg gctcggagtt gaccacggcc acgatgtagt
     4981 cggcctgccg ttgcacaccc gccaccttac ccagggtgcc cagacttggc acccgcaact
     5041 ggccaggcgg cctcaagacc tgggcctgcg cggaaagggc gggcaagccc agcaccagcg
     5101 ccagcgcggc gacgcagcga aagccaggac ggggggggaa actggattta gtcatagttg
     5161 gtaaagcggc tgggtgtcgt gctctgctca cgcaggtact ggtaacgcgg gatgttggct
     5221 ttgagggttt tcaggggatt ggaaccaatg cgcgagaaac cgacgaactc caattggaac
     5281 aggatgcgcg tgttgttgga tgagcttcct gccagaacct gccccagttg ggtacgctcc
     5341 aacacaacgc ggccgatcca gcaacagccg tcgtattcaa tgcccagcac catgtccaca
     5401 aatttcttgt cgggcacact gtagttcagg cgaccgacac tgtaccagcg cccggcgccc
     5461 tgaccacgac cggcgcccag atccttgccc ttgtcacccc acaaatcatt gatcggccat
     5521 tgccaaccca catcaaactg ttcgctgaca ttgcgctggc gacggtaggc agcgctgatg
     5581 acgcggtagt tgcccgggtt gtagcgggca cccaccgtcg aacgctggga aatattggtt
     5641 ttcgggttgt actggaccgt gctgtccagt gaccaggcct tcgtcaagtt caccgagcca
     5701 ccgaacagca ggtcgctgaa acggtccgtc accggtaccc ccccaggcaa ggtgacctgc
     5761 tggtccttga agcgcaggcg ctgggccacg cccaaacgca aggcctcggc accggtgtca
     5821 ggatccagca ggcgcgaggt cagacccagc gtcagcaggt tggcgtctga aatgcgatca
     5881 ttgccgacaa atgcgttctc ggtgaacaca gtcgcgaaat tgaaatcgtt cgctcccgag
     5941 tcatagttgg gcagagaact ctgatcacgg tagggcgtgt tcacataaaa cgcgcgcggc
     6001 tccagcgttt gtgtataggc gcgtccgaaa taaccggcct cgcgctcgaa ggtcagcccg
     6061 ctgtccaggc tgaaggttgg taccgtgcgc gaagccgagc gtgctccatt aaccaacgca
     6121 ccgtcaaact ggtaggtggt ggtattgagc atcagcttgg gcgtgatgta acctccaggc
     6181 cggatccagg gtcggctcac ctgcgcgagt gcaaacgcac ggctggcgtt gggctgcccg
     6241 gtcaatcgcc gatccgactc aaaccgcgtg tagtccgtgc tgattgaata gtcgaggccg
     6301 cccaatcctc ccacaggcac attggcacgg gtgtaggccg cagtgagctg cggcctgcgg
     6361 tcataaggtg gcacgatggg cgaatttacg tcctgcaggg tctggtaggt cagggtgcgc
     6421 agggaagtcg aaaaaaagcc acgcccccag gtcaggctgg cgtcgctggg cagcagtcgc
     6481 tgggtcaaca aggtactcgc gcccgcactg ccaaaccccg aaaaatcgcg ccagtaattg
     6541 tcgtcgctga cccggttcaa attgaggttg atgcctacac cgccgattgc cggtatgccc
     6601 gtgttgaacc caccactatg caagtagttg taggaccagc ggttgctgtt gcgcaactgg
     6661 tcattgggca tcaggctcgc gcgcaattca cccttgtagc cgggctccag ataacgaaac
     6721 tcgccggcca gatccacccc gcgcttgctc atcaaggtcg gcgagaaggt ggcatctcga
     6781 ttaggcgcaa tatcgaaata atagggcacg gtcacggccg tgccactgag actgtcgatc
     6841 gcaagcgtcg gcggcaacac gcccgacttg cgcttgtcgc tgagcggaaa gctgaaggta
     6901 ggtgccgcca gaatcggcac attcatgaag cgcaggacac cgcccgaagc ctcgccgaca
     6961 tccgcttccc tgtcgagctt gagtgtggtg gcattgacga tccacgccgg catccagctc
     7021 ggaccctcgc cgcgctggca ggtggtgaag gtggcgttgt gcgccaccag acgcttgtcg
     7081 tccagaaaat cgatgcggtc ggctgcgcca taaccgtcgt tctgcaagaa ccgatagtcc
     7141 ggcgtcagga aaaaaccctc gaaggtgtcg agcttgattt ccagctccgg cccgttgtac
     7201 aggttgcccg agctgttgac gcgcacattg ccccgggcct tgacaacatc ggtcggctgg
     7261 tcgtactcga tccggtcggc gcgtatcgcc gagccgccgc ggcgtagctc ggcctccccg
     7321 tcaaccacgg tttcgagatc cggacggccc gacacgcggt tgccaaaaac aaaggtcggc
     7381 agttcgatgc ccggaccgcc cggcggctgc tcggccagca tggaagtgct cctgagcagc
     7441 accggcgcac catcgctggt ttgaacaccc ggcagttcct gcgcctgccc cggggctgcc
     7501 gccgggcttt gggcataggc cggcccgcca tgccccaagc ccagcacggt gcaggacacc
     7561 aggctgagaa cggcatgagc gaccggagaa tgaataaaac gcgagcggga tgcgcgatgc
     7621 atcggaagta tgagataaac ccgtttgtga aagctggccc gattatccta tgcccacccg
     7681 accctttccc catcttttga tgtatccatt taaggcaggc cgaaagaccg catgaccgaa
     7741 tcccccattg tctggcctga tgccacccgc gccggtgctt tttccgcctg gctgcaggcc
     7801 catgcctcta cccacggcct gctgccagaa acggtgcaac cagcatcggc cgacgccagt
     7861 ttccgccgct atttccgggt gcgtaccgcc gacggcggca gttgcatcat catggacgcc
     7921 ccacccgcgc aggaggactg cgcgcctttt gtcaaggtcg ccgggctgat ggcgggcgcc
     7981 gggctgaacg cgccgcgcgt gctggcctgg gaccaggccc agggcttcat gctgctgagc
     8041 gacctgggcg cccggaccat gctcgaggtg atcgagcagc aacccgaggc tggcaaggcc
     8101 cagcgcgcct acggccttta ccgggaggcg gttgatgcgc tggtccgctg gcagctggcc
     8161 tcgaagcccg atgtcctgcc gccctacgac gaagccctgc tgtcgcgtga gctggcgttg
     8221 tttcccgact ggtatatgga aaaacaccgc ggcatcacca tcgacagtac cctgcgcggc
     8281 aagctcgacg gcctgtttgc gcagatcaag gacagcaacc tgaattcgct ggggggcgca
     8341 cgggtgtttg tgcaccgcga cttcatgcca cgcaacctga tggttccgga aagcggtgag
     8401 ccacggttag gcgtgctcga ctttcaggac gcggtttacg ggcccatcac ctacgacatc
     8461 gccagcctga tgcgcgacgc cttcatcagc tggccggaag acttcgtgct cgagatcacc
     8521 gtgcgttact gggagcaggc gcgcaaggcc ggccttccgg tgggggatga tttcggggag
     8581 ttttaccgcg cggtggaatg gatggggctg caacgccacc tcaagattct tggcattttt
     8641 gcgcgcctga ccctgcgcga cggcaagcct aagtacctgg ccgacacgcc gcgtttcatc
     8701 acctacgcac gttcgacctg tgcgcgttac cgcgcactcg gaccgctgat ggtgctgctc
     8761 gacgagatcg aaggcaacga gacccgggtg ggctacacgt tttgagcctt ctctccaacg
     8821 tcgaatccgg cccggtccct ttggtaccaa gcacggacag ctacatattt catagcaaca
     8881 tg
//
LOCUS       DAIDFG010000110         8817 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_110_length_8817_cov_19.346040, whole genome shotgun sequence.
ACCESSION   DAIDFG010000110 DAIDFG010000000
VERSION     DAIDFG010000110.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8817)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8817
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110_length_8817_cov_19.346040"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1671 a         2989 c         2742 g         1415 t
ORIGIN      
        1 cgcggtcggc cacggccagc gctttcttca cgttctgttc gaccatcagc accgtggtgc
       61 cggcgtcggc gatgcggcgt gtgatcgcca gcagctcatc gaccatgcgc ggcgccaggc
      121 ccagcgaggg ctcatcgagc atgagcaggc gcggcgcgct catcatcgcg cgcgccaccg
      181 ccaccatctg ctgctcgccg ccgctcatgg tgccggccag ctgctgcgcg cgttcgcgca
      241 gcttgggaaa atcgttgaag gcctgctcca ggcgccgggt gcgttcggcc ttggcgacca
      301 gccagccgcc gagctccagg ttttcagtca ccgtcatctg ggggaacaac tgccggcctt
      361 ccggtaccag cgccatgccg cccttgacga tgtcactggt tttttcgggc agcgcttcac
      421 cgttgagcag cacctcgccg ccgcgcggaa tcaggccgga cagcgccttg agcaaggtcg
      481 tcttgccggc gccattgggg ccgaggatga cggtcaggcc gggtttgatc tccagcgtca
      541 gatcgcgcag caccaggaag gcgccgtaac ctgcgctcag gccggcgact ttgagatgcg
      601 catgcgcgga gccgccgagc acgaaggaag gggcatgcca catcatgatg cagctgcctc
      661 cctaaatatt gtggtcaagg ccacaccgcg gcgtcggcag caggcagcat tgcgctggtc
      721 gatcccgctg cgcggggccc tcgcccttcg cttcatacag cctccccctc ggcttccacc
      781 atctcatccc ccagataggc ttcaatcacc tcttcattgc gcaccacctc ggccggcgtg
      841 ccgtcggcaa tcttgcggcc ctggtgcagc acgatcacgc gctccacatg gcgcagcaag
      901 gttgtcacgg cgtgctcaat ccacaccacc gtcaggtcca gttcgtcacg catgcggcgt
      961 atcagctgcg ccatgttttc aatctcggct tcggtcaggc cggccgccac ctcgtcgagc
     1021 agcagcaaac gcggccgcgt ggccagcgcc atgccgacct cgagcaggcg ctgctgcgac
     1081 ggggtgatgg cgcccgccgg caaggccgcc tgggcctgca ggccgatcag ttcaagaatg
     1141 ctggtttcgt cgcgcagatg ccaggagccc ttgcgcgttc gcccggcaaa cttcagtccg
     1201 aagtcgatgt tggcggcaac gctcatgtcg ggaaacacgc gcggcgtctg gaaggtgcgc
     1261 gccacaccgg ctgccgcata acggtgcggc ccctgccccg cgagatcgcg cccggccgcc
     1321 agcagctgtc cgccggtcgg ggggatcacg cccgaaatcg cattgaaaaa ggtggtcttt
     1381 ccggcgccgt tggggccgat gatgccgacc agttcgccgg catggaagtc ggcgctgacg
     1441 gcatcgaccg ccgtcaggcc gccaaaacgc accgtgatgt cacgcgcctg cagcagcaat
     1501 tcagccattc ttgcgctcct tgcggctcag gccacgcgcc cagccgcggc tgtcacgcca
     1561 ccagccgctg aaggtggccc agcgcagcga ggccaacccg cccggcaggt aaagcacgct
     1621 caaaatcagc agcagcccca gcgacatcat gtagacctgc ggcgcctgca ggcgcagcgt
     1681 ctcggccagc acgctgaaca caatcgcggc gatcagcggg ccccacaggg tcatcgcacc
     1741 gccgatcagc gcaatcagca cggtctggaa accgatgaag gggttgaaca ccgtgtgcgg
     1801 gtcaatgtag gtccagcgca ccgccatggc cgcacccacg gcgccggcaa acgcggcggt
     1861 catcgcaaag ccggccacct tgatcagccg cgtgttcacg cccagggtct gcgcgcgctg
     1921 ctcgtcggcg cctatgccca tcagcgccag gccgaagcgg ctgcgccgca cgatgatgga
     1981 gaccagcacc gcgagcacgg cgagtgccag caccgtcagg taaatcgtgt cgcggtcggg
     2041 taccacggtc agcacccggc ccaccgtgcc ggtgactttc ttttcaaagt aggtcaccgc
     2101 atggcggatc agctcggtca tgccgaaggt cagcaccgca aagtaagtgc cgcgcaaatg
     2161 cagcacagcc gcgcccatga tcacagccac caccgtcgca atgccggcgc ccagcgcaat
     2221 cgactcgagc caggccattt tttccagcgc aatcgcactg gtgtaggcgc cgatgccgaa
     2281 gaaggccgag gtcgccagcg agaggtaacg cgtgctgccg cagaacaggc cccagctcga
     2341 ggacagcgcc acgtacatca ggcaggtcag cgccatcgag acgacaaact cgctgccgta
     2401 atgcggcagc gccagtgccc agccggtaaa ggccagcaac agcagcaggt cgcgcaccag
     2461 aatctgtttg tctttcattc ttcttctact tcgcaaacag gccgcgcggc cgcaacaaca
     2521 ggaccgcaat aaacacgctg taggacagca gcgccttcag cgaggggttc gtgaaatgca
     2581 tgcccagcgc ttccaccacg cccagcagca aaccgccggc cagcgcgccg ctcatgctgc
     2641 cgaagccgcc cagcgtgatg acgatgagcg cggtgacggt atagggctca cccatggaag
     2701 gcgtgatggt gtaggccatg gacaagaggc agccggccac acccgacaac cccaggccga
     2761 tgccgaacat cagcggatgc agacgctgcg tgtcgatgcc caccagctgc gcgccggtcg
     2821 gcgactgcat caacgcacgc acacccttgc cgagcagcgt gaagcgcagc aacgcaatca
     2881 gcgcgccact gaacagcaac gccaggccga acaccagcac cttgttgccg gcaaactgcg
     2941 cctcgccgat cttcaccggc tcggccaggt agtcgtagcc gcgcaggtcg ccgccccaca
     3001 tgaaagaggc gaagttctgc accaggaaca tcaggccgaa ggccaccatc aggccgcgcg
     3061 cctcgaagat gtcgaggttc ggcgatgtga tggtcaggcg ccggaagcac agcttgtgaa
     3121 tcaccaggcc cagcgccatc agcgccagaa aggacaccgg caccatcagc agcggcgaga
     3181 tgccgagcgt ggtgtgtgcc atccaggtca ggtaggcgcc cagcatcagg aattcgccgt
     3241 gcgcgatgtt caggatgcgc atcagcccgt actgcaggtt cagcccgagc gccaccagcg
     3301 cgtagatgcc gccggtgatc agcccggaag cgatcagttc aagccaggaa aaaagtgaca
     3361 tggtgaaaaa ggcagccccg ttcgcaccga acctgtcgaa gtgcgcgggg cggcttcgac
     3421 aggctcagcc tgaacggggt ggtgttggca ggtcggagaa tttatttcca ggcgggcttg
     3481 acgaggctaa gcttggcagt ggccaccttc tgcggccaca ccacctcgaa ctcgccgttt
     3541 tgccactggc ccacggtgcc cggcgtggcg gtgttttcac tgccggtgaa cttgatgtcg
     3601 ccgatgatgg tcttgtgcgt gttgccggcg atgtagtcgc ggatcgcctt gcggtccaga
     3661 ccggacttgg ccacggccgc ctgcaggatc tccaggccgg cccagcagtg gccgctggcc
     3721 cagcggtccg gctccttgcc gccgaatttc ttggtgtgtg catcgaagta ggccttcgcg
     3781 cccgggctgg tcttgctgtt ccacgagccc atgccgagca cgccttcggc gccggccggt
     3841 gtcatcacgt tgcggtaaag ctggaaagcc gtgccgaccg aggcatagta aaagcgcggg
     3901 ttgaagccga tttccttgga ctgcttgctg gccaggatgg tgtcgggcgg gtagctcagg
     3961 ccgatgaagg cctccggctt cttgtccttg atggagcgca gcaccggcga caagtccttg
     4021 atgtcgccgg ggtagctttt ttcttccacc acctgaatgc cggtgctgcg caacgcgacc
     4081 ttgagcgccg agaaattctc cagcccaaac aggtcgtcca catagatgac ggccaccgtc
     4141 ttcacgccat tggccaccag catgtccacc agtgcgccga ccatgcggtc gggctgctgc
     4201 agcagcaaaa agaagttcgg cagcttgagc tcgaccagct tgcgcgacag cgccgtcggc
     4261 gccagcaccg ggtaacccat gcggttggcc agcggcgcga tcgcgaagtt ggcattgctg
     4321 ccccagggtg gcaggatcaa atcaaccttg tcgctgccca tcagtttttc atacgaacgc
     4381 acgcaggtct cgatgtcgct gcggtcgtcg gagctgatca gctcgatcgg gcgtttcgtg
     4441 cccttgacgt ccagcccacc ggccgcgttg acctgctcgg cccagagcag gtagttgggc
     4501 tcctggctga cctgcgcgcc gccggtccac gggccggtgc gggcaatggc gtagccgatg
     4561 cgcaccgggc cggcttgggc aaacagggag ggggcaaagg cggcggcgcc gacggcggcg
     4621 gcagcgccct tgagcaattg acgcttggat gtctggacca tggtgtctcc tgtgtagtcg
     4681 ccctgaaggg ctgttggaac ccgcatgata ggcagacacc ggaccgggcg ctttgctttg
     4741 cgcgcacaaa gcagttgacg gtcagcgcac ggcgatccgg gttaaccctt gacccgtaac
     4801 tgcaacagga cagggcacaa cacccataat ctttgccatg ggccgagccc acaaaaaccg
     4861 gagacaagca tgaccgcggc caccctggta tcgaccaacg acttcgcccc gcgcgagcgc
     4921 gcaccggtgt ggcgcgactg gatctggaaa catttcggcg gcctggaatc cgacctctac
     4981 ggcgacaccg atttcgacgg cgaaatgatg tctgcccagg cgggggacgt ggtgctgacc
     5041 cggctggaag ccaaccgcca ccgcgtcgtg cgcaccccgg acatggtgcg cgccagcgat
     5101 gcgggttacc tcaagatcgt ggcgccgctg cagggccgcg ccggtgtcga gcagatgggc
     5161 cggcgcgcct gggtcagccc cggtgcctgg accatttacg acaccaccgg ctcctacgcc
     5221 gtggacaacc ccgaacgggt ggaacacctg atcgtgatgc tgcccaaggc gcagctcatc
     5281 gagcgcggcc tgccgctgga gaacctgatg gcacgccacg tgggcggcgc cagcggcatc
     5341 gcccgcgtgg cgctcaccac catgcgcagc acctaccagg agctgcccaa catgagcgcg
     5401 gccgccgccc gcggtgccgg cgaactcatc gcccagctgg tgcgcctgtc gctgatcgaa
     5461 ctggccggcc aggccacagc cgtgacgcag cgcgaagccc tgaaggaccg catccgcgcc
     5521 tacgtcagca cccggctgcg cgacccggcc ctgtccatcg agcagattgc ccacgcgctc
     5581 aactgcagca agcgccacct gcacaacgcc ttcgccggcg aaaccgacac cctggccagc
     5641 tacatcctgc acctgcgcct gcaggcctgc atccgcgagc tgcagcaaag cgggccccag
     5701 gcgcgcccca tcaccgacat tgcgctgtcc tggggtttca gcaacctctc gcatttcagc
     5761 cgcgtgttcc gcgggcacac cggcctgagc cccagcgagt tccgccaagc ctggctgctc
     5821 aacgcggctg ccgggctgct gcgccactga accgggccag attcagcatc aaatgggccc
     5881 ttagccctta ggggacgtgc gctgacagct cttcttttga tagcattcca ggatgacccc
     5941 ggaccggtca ggcgcttgtc aaacgcgtga ccgtcgccgc cccgccgctg gcgtatcgtg
     6001 cgacatcacc ccattcagga gacccctcat gcatattccc tccctcaagg aagtcgtcag
     6061 cgccgaagaa tggcaattgc gctgcgacct ggccgcctgc taccggctgg tggcgctgta
     6121 cggatggagc gacctggtct tcacgcacat cagcgccaag ctgcccgaat ccgtcagcgg
     6181 cgccggcgcg caccacttcc tgatcaatcc ctacggcctg atgttcgacg agatcaccgc
     6241 gtccagcctg gtcaaggtcg atgaacaatg caacaaggtc atcgactccg ccttcccggt
     6301 gaacccggcc ggcttcgtga ttcacagcgc ggtgcatgaa gcccgacccg acgccggctg
     6361 cgtgctgcac acccacaccc gcgccggtgt cgccgtcagc gcgcagaaat gcggggtgtt
     6421 gccgatcagc cagcagtcca cctttgtgct gggctcgctg gcctaccacg cgtacgaagg
     6481 cgtggccttt cgcgacgacg agaagccgcg cctgcaggcg gacctgagca cggccaactt
     6541 cctgatgctg cgcaaccacg gcctgctggt ggtcggcaag acgattgccg acgccttcct
     6601 cagcatgtac accttcgaga acacctgccg catccagctc gacgcccagg cgggtggcga
     6661 gctgatcgcg gtgaacccac agatcgtcaa gggggtggcc gaagccatgc gggtgcagac
     6721 cggcggcctg ggcggcgcct ttgcctggcc ctcgctgatc cgcaagctcg accggatcga
     6781 cgagggttac aagtcctgaa gacttgacca gagacgctgg ctgttcaggc agccatggac
     6841 aaggccgacg gcgagttatc ggccgcctcg cccgcaggcc tgtcgccctg gtccaccacc
     6901 agctccttgc gcaacatctg cacgttgccg aaaattgtgg caaaggccac gtccttggca
     6961 tcgcgcccgg tgccgacgcg caccaggcgg tcgttgggcg ccatgcgtgt cgcatcaaac
     7021 agcacccagc ggccatcgag ccaggcctcg aacaccgcgt ggaaatcctg cggcggctcg
     7081 tcaaaataga cataaccgac caccagccgc gccggaatgt tcagtgcgcg gcacaaggtg
     7141 atgcccaggt gcgcaaagtc gcggcagaca ccggcacgct gcacaaacac ctcctgcgcg
     7201 gtggtggtgg aattgctgaa gcccggcagg taatggatgg aatcatggat ccagctttcg
     7261 atggcccgca cccgcgacag gcccggcgag aggtggccga acagctgctg cgcggcgcgg
     7321 ctcatgatgt cggactcgca gtagcgactc ggcatcaggt aacgtaaaac gtcgtcgggc
     7381 acggccgtca ccgcggcctc ttccaggtgc aagggcactg gcgcatggtg aagctccacc
     7441 tgcgcgtggt agttcaccga caactggccg cgcgaggcgt ccagccggaa aaaacggttg
     7501 ccgctacgct cgtcgcagaa atggcgcacc ttgacgctgg gcgtcaccgt cagtcgctcc
     7561 tccaccacgc gatgcgcctc gtgaaaagcc gcctcgatgc tgaagcagaa atgcgtgggg
     7621 ctggcgacgt cgtagtccag caggcagtca atcgtggcgg tgtgcatcaa ggtccttttt
     7681 cttgttgtag tcggcgggcg gtgaaaccgg ggcgccggtg gccaccgggg gcacctgccc
     7741 gtcccggtcc ggcgacggca gaaaccagtt cttgcaggtc acgaaaatca catgggcggc
     7801 gcgttccagc aaatccaccg tcaggctgcg ccgggtgagg gcgcgcagtc gcgtcaccag
     7861 cagcagttgc gcaatgctgg cccgcaggcg atccacccgc cgcactgcta catggcgact
     7921 ctggtcggaa ggcggggtcg ggggcacctg ggacatgaag ccgatatcca ctgacagcgg
     7981 catttacttg agcgccttcc tggccgcacc cacaccgagc gccgccaaca cgataaacac
     8041 cacggccaga atcaggaaca ggccgaacag gatcttggca atgccggccg agccagccgc
     8101 aatgccgcca aagccgaggg ctccggcaat cagcgaaatg atggcgaaaa caatggcgta
     8161 cttcaacatg gtgtttctcc ctgaaaagga tgaaaaggcg ccaggcaaga cggtgccagg
     8221 caagtcttgc gacaatcagg acaatggtag gcggcaggcc gcgcgcgtcg aatcagcagc
     8281 cggcgcacgc cgctgtagga caaaccggac aggccgccga actcctcttt tcatagctgc
     8341 cggcgcaggc aggaaaaggg ctgatgccgg ttttaaccac aagcccggcg gccgggcgtg
     8401 gcgccagccc tgcttgcaga agagttcgtt caggccctcg ggcagggaaa tcttcatggt
     8461 gctcatggac tgaagcctgg cactttgagc aaatttggca caaacaggcc gcatcggtcg
     8521 gcgggcctgt tttcccctga tcaacctctc tttgggccag atcaaacgcc cgcgacagga
     8581 atacgctaac ttgcctgaac cagcttgcgc ggataacacg aaaccagcag ccccgcggac
     8641 ccccggcaaa ttcaaaacag cccacactgc aggttgttgt ccctttttca tcggaaccct
     8701 ttcatgccta ctttgttcga ccccgtccag gccggtgacc tgcaccttgc caaccgcatc
     8761 gtcatggcgc cgctcacgcg caaccgcgcg cccgacgcca tccccacgcc gctgatg
//
LOCUS       DAIDFG010000111         8738 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_111_length_8788_cov_18.422993, whole genome shotgun sequence.
ACCESSION   DAIDFG010000111 DAIDFG010000000
VERSION     DAIDFG010000111.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 8738)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8738)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8738
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111_length_8788_cov_18.422993"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1794 a         2703 c         2609 g         1632 t
ORIGIN      
        1 aagaagtcgt tcaacactga aacaacatcg cgcggcgcag caggggcgcc acagccattg
       61 aatggtcgtc gggacggttt cctcctacaa aatgaggagg taaaattttg ttacaccgtc
      121 tacgatgagg ctcccattga ttgaactccc tgctgccatg acaagaaaca cgaaagtgtt
      181 gctggtcatg gacgtggtgg agtcggtcag gatcatggag caggaccagg atggctttgt
      241 acggcggtgg caacaactcg tggaagaagc ggagcagaac gtgctgccgc agcatggcgg
      301 gcgcattgtc aaaagcctgg gtgacggcct gatgctggaa tttgccaacg cccaaagttg
      361 cgttaaagcc gccttcgccc tgcacagttt cacgcggcag accaataacg gcttgcggcc
      421 cgagcaccag atgcacctgc gcatgggcgg ccacctcgca agcttcgtca ccgaccagca
      481 cgacatctac ggcaccgacg tcaacctgac ttcgcgcgtg agcacgctgg cgggtccggg
      541 ggaaaccgtg gtgacggccg acatgcggga tcaactcgcg ccgctgctgg acgcagacgt
      601 cgaagacctc ggcgagtgcc acctcaaaca cgtcaaggag ccggtgcgtg cctaccgcgt
      661 cggcccgccc ggtgatgcac ccatcgtccc gccgggcaac gcggtcgagc tgaacctgcg
      721 gcccacgatt gcggtgatcc cgtttgcgat gcgcagcaac gagcccggcc atgaactgct
      781 cggtgaagca ttggctgacg aggtgatcgc ggcgctgtcg cggacctcgg aattgcatgt
      841 gatttcgcgg ttgtcgacca cggtgtttcg cgagcggcag gaggctgtcg aagatattcg
      901 gacgcatctg ggagccagtt acattctctc tgggacctgc cgcagcatgg gcgcccaatt
      961 ggcgctgttt gtcgagttaa tcgatgcaaa gaccggccac gtggcgtggg ccgacaacct
     1021 gaaaggccag gtacacggac tgtttggtgc agacgacgca ctgatcgctc gcctcatctc
     1081 tgcaataagc actgcggtta tgtctcaaga attacagcgg gcgcgcagcc atgcattgcc
     1141 gacacttgaa ggctacacct tgcttctggg cgcggtatcg cttatgcatc gcaattcgtt
     1201 cagcgacttc gaccgggcac ggcagatgct tgaacacctg attgaacgca gccgccggca
     1261 cccggtcacc tacgcatggc tggcaaaatg gcatgttctc aaagtgcagc agggatggac
     1321 gcaaaatccg gcacaggaaa ctcagctcgc gcagcgctgc atccagcagg cactggaaaa
     1381 tgatcctggc tgcgctttgg cctggacagt cgacggcttt atccacacca acctgttgcg
     1441 ccaactggac gccgcagacc accgctacgg ccaggcctta tcgctgaatc cgaacgactc
     1501 gcttgcatgg ttgttgaagg gcatgctgcg agcatttgct ggcgagggcg cccctgccat
     1561 ggagagcgtt acgcgtgcaa catcgctgtc cccgctggat cctctgcgct atttctatga
     1621 ttcattaacc gcgtcggctt gcatttccaa tggccaatac gaagagtcga ttcggagcgc
     1681 caagcgatca ctgcagctga acatgatgca cacgtcgacc tatcgggcgc tcgcgatggc
     1741 ccaggctctt acgggcagac tggcggacgc gaaacaaacc gtcaaccgcc ttctcgatct
     1801 ggaaccaact ttcactgtcg caaagttttc gcaacgctat ccgggcaaag acatcgcgcc
     1861 ggactacgcc gcaaagctgg ccgatgcctt gctcatggca ggtgcccccg cgcactaaat
     1921 tcaagattta tctaacccaa caatcaggag gggcactatg tctattatgg gtggctacgg
     1981 tggttatggc ggctatggcg gctacggtgg ttatggcggg tacggaggct atggcgggta
     2041 cggaggctac ggtggttatg gcggtcccgc tccggaagcc ggccccttgc tcgcggaagc
     2101 aatgaccaaa agccggggtg cccttgtggg accttttcaa ggtgcgaccg taccctttac
     2161 cctgccggtt cccttggaca aaccagaccg gctggcggag tggagtgttg taccgcgctt
     2221 gcaagcgtgt gaactggagt ttctctccgg cctgtacttt gatgtcaacc agtcacgtga
     2281 tgcgatgacg gttggttttg ttgatctcaa caaaaaatct ctcaagagag tgcagttggc
     2341 tcgactcgtc cggccaacgg catccatatt ccgccagcaa gtcgatctgg tggcaaatta
     2401 cgcagaactt cgcgaggaca ggggtagcga agtcttggcc caaacctcac cttcgcatgc
     2461 gcctttttgg ggctccatcg tcggtcttca ggatcaccgc cataagtgga cacaggagct
     2521 gatcactctt gcgttgatgt tggtggtcga cgtggagatg cggttcaaac acgccttcgc
     2581 atgtccccgc ccaatggcat tgtcacctca gattcaacca atgattccga ctcccgggca
     2641 cggaagctgg cccagcggcc attcgaccga ggcttttgcg gttgtcacag tgttggaggc
     2701 cctgatcaac agcgccaacg gaacgggggc caaatataac gaacagttgc agagacaagc
     2761 agcacgcatt gcggtgaatc gcacggtcgc cggtgtgcac tatcccgtcg acagcgcggt
     2821 cggacgaatg cttggtaccg cactggcaga cttttttgtt gctcgctgca caggcaagat
     2881 caagcttcac gaacgcggat tcaacggccc caggtttcac ggccccaaag accaggtgct
     2941 ggacttcgat ccccgcgtgt ccatgatcga caacaagagc ggctattacg agtacgggtc
     3001 aacggcatcg gccattgccg cctcaccgct gctcgaattc atgtggacca aagccgccaa
     3061 agagtggcag ccgctcaagt aagccgccat gagctggacc aaactgccca agggcgagtt
     3121 cacgggcatc gattgggcca agcccagtgc caccaacggt tttgaccctt acctggtatg
     3181 ggccgaggct gacaactttt cgggctacgg caacaagcgg ccaaagtggt tgccggttgc
     3241 catcgaactc aagtcgggta cgacggtgca acagcttatt gctgccgcgt cgcccaaatg
     3301 gctgcatgtg ccgcccgctt acaccagccg cgctgcgccc aagggcttgc gcttttgcac
     3361 ggcgcgcgtc aggccggcgt ttttcaaaca gctgcagccg ggcggcagtt tgcatgcgct
     3421 ggtgctgcgg cttgagttgg gcttgcctgc ggcggagtat gccgacgact cggcggtgaa
     3481 tccgccggca aacccgcccg tgcattcgag cggcccagcc tggcaggccg agactcaggc
     3541 cgaaggtctg ctgtccggca acgtcctggc gctgatcgac gacagcctgg cgctggctca
     3601 tgccaatttt cttcatcagg gcaaggcgcg cacccgctac ttctggcggc aggacgccca
     3661 aggcaagggc cgcacaccgc aaagcatggg ctatggccac gagttgaccg ccgccgacat
     3721 caatgccgcc atgcaggcca acacctacaa cggtttggtg gacgaaagtg ccgtctacgt
     3781 tgcgctgggg ctgtccaccc tgggcagaaa aatgcccagg cgcgagcact tctttcatgc
     3841 gctggatacg tccgtcagtc acggcaccca tgtcatggat ctggcggccg gtccgcgcac
     3901 gctgctggcg caagttgcca atttgccgcc cacgtttgac gcaccgccca gctgggcgct
     3961 ggccgatgac gatgccagcc gggcggcctt gattgccgtg caactggact atgacacggt
     4021 gcgcgacacc tcgggcggct caatgaacgt gcatgtgctc gacgggttga tgtatgtcct
     4081 gtcgcgctgc gcggacgacg ccaaggtgac ggccaacatc agcttcggca cgctggccgg
     4141 cccgcatgac gggacatcgc tgctggaagc tgccatggat gagctgattg gcttgctgca
     4201 ggggcgtctg cagatcgcac tggctgccgg caacagctac cagctgcgca cgcacgccaa
     4261 tgcaacgcta ttaaaaaaag agcgagctgc gcttcattgg cgggcgctac ccgacgattc
     4321 gacccaaagt tttgtggagt tatgggttga ggaagaccgc gacggtctgg agatcaccat
     4381 caccccgccg gggcgcgcgg ccttgccccc gctgaagttt ggcgagtcga gaatgtggac
     4441 gggcggcggc aagcaaccgt tgtgcgcgct gatataccca aagacagtcg ctaccggccg
     4501 acgcggcact tgcgccctgc tcgcggtcgc gcccacgttc agttttgacg acaagacggc
     4561 gaccgcaccc agcggggtgt ggcaaatcac gctgaccaat accggcaaga aggcggtcac
     4621 cgtggacgct tatgtggagc gggacgacgt ggtcatcggc acccgcggtg gcgcccgaca
     4681 gtcacacttc gaggacgcgt ggtatgacac cagcgggaac ccggactcct tcgtggacca
     4741 tcccgacaac ccaagcctga tccggcgcag cggaagcttc aacagcatcg ccacaggcag
     4801 caagaccgtg tcggtaggcg gtacgcggat caccgggccg cgctgggccc actattcccc
     4861 gcagaaacct gaccccgatg cagggcgggt tcagcgcccg ggggtaacca ggattcccga
     4921 tgcgcaagcg ttcagcgacg agaacccggc aacgctgggc gtgaatgcgg ccggaacgcg
     4981 cagtggcgcg gcggtacgcc tgatgggcac cagtgatgcg tcaccccagg aagccaggcg
     5041 gattctgaac ggcatgtagg cttggcagga gatgcagcga aaccgggtcg gtgtgaatca
     5101 cgggcctgtg aaggatccgc aagcggacgg gtactttcgg caaggccgca gctgttgaca
     5161 acaacgcaac actttgcacc gtggcgtcgc tcgctgatgg cctaaagctc ctgcccccgg
     5221 ttcaccctct tttcacggga gagagttggc cctacactca gcgtggattt acagtacgtt
     5281 gcaattttta cgccgaacca gcaatttcat caaccgtaac cgcaggagag atttatgggc
     5341 tatttcatga aagagcatga catccacgtt ggaagtctgc ttgaccaatt gaatgtacgt
     5401 ttcggaccaa cgcctgacaa gaaaacgcat tttggtggca tcgacgaaat ggtggcactg
     5461 caaaaagagt tcaagatttt caaaaagggc cgctcgttca agagcagcgt ggcagtcctc
     5521 aatatcggtg cgtccaatgc cgaggccaag aaccgcatgc acgactattt caccaatctc
     5581 agcaagcatg actcgaatgt cgctggccag aacggcgacg tggccattgt caacgccatg
     5641 gcgcgcaacc ttgcttcaaa aagcccgctg ccggtctatt tcacatttca tgacatgcgg
     5701 gcagaaaagg gcaacacacg tgtgctgatc aaggagaagg accggccggt cgcctatttc
     5761 aaccaggatt acctgaccat ctccctgccc accatggcgc tcaaggcagc aaaaccaaca
     5821 gccaaacctg cggcaaaagc ggccaagaaa acggccaagt gatgaaagcg ggggcgggca
     5881 ccatcgccat caccagggcg tcctcccccg atccgcggct tgtccaggtg cactcgggca
     5941 ttgagcgtta ttactcacaa aaagttctcg cacatggcgc gactgccctc ggggtggact
     6001 ggacttgcca gccaacccag gaacttcgct ttgtgcaact catcaagttg tgcgacttcc
     6061 gccagccgtt ctcgctaaac gatgtgggct gcggttatgg cgcgctgctg gcctttctgg
     6121 ccaaacggca tcggggcgca aaggtggact acctcggcac cgacctctct caggccatga
     6181 tcgaccaggc caaaccgtcc ccgcgcccct ccatgcgctc tcagtttgtg gtgaccggtg
     6241 acattccccg cgtggccgac tactcggtcg ccagcggcat ttttaatgtg aagctgaacc
     6301 agcccacggt ccgctggacg cttttcatcc agcagacgct ggccgggatg catgccgcaa
     6361 gccgtcttgg ttttgcagtc aacttcctgg cgcctcctgg gccggacatg caggccatcc
     6421 cagagctcta ccgtgtgtca ccggatgtgt gggcaagcca ctgcgagcgg cagttcggag
     6481 caaaagtgga gctgatcgaa ggctacggca tgcgcgaatt cacgctgctt gtccgacgct
     6541 gaccccagtg ttttcggggg gctgatggat cagcatggcc agacgggccc gttcgcgcag
     6601 caaggtcgac tcataaatcg aggcaccggt cagctgcacc agcgcctcgg cctccgcaaa
     6661 cagggcctgc gcatcggcca cccgcgccag cccgtgggta tccagcgttg ccgatgccag
     6721 ggtgatcagg gcgcgacact ccggcagccg cgtactgcgc tggcgcgaaa tggcgatcgc
     6781 ctcactggca gcggccaggg cttgctcaaa actccccaag ccccgataac actcggcgag
     6841 gctggccagg atttcggttt caaactccat cgcaacctgg tcactcctga caagtgccaa
     6901 tgcctccgaa taggtacgtg ccgcagtgtc aaagtcgcca ccaatgcttg ccaccgtagc
     6961 gcgacaactc aacgcaaaaa cccgcaggta aggactttcg tattttgcag cgatctcggc
     7021 gacataagcg taatgtgcct ctgccagcgc ggcatcgtgg cgacaccaag ccagttctat
     7081 atagccgaag cgggcgaact gcaggatcac cgggtcattt gatgattgcc ccatgtccag
     7141 caactccttc aggcactgct cggcttcatc cagacgcccc agccgcgtca gcaaccggcc
     7201 ccgcatcccc atgacccagt gttcgatgct gaagccaatc cattcgcggt cgaacctgtc
     7261 aatctccccg atgtcacgaa gcgcgctgct gtttgcctcc agggcttcgc ccagcatgcc
     7321 ggcccaggca taggcctgac tcaatgcggc attgatcatc gcagcgcgcc ccgggtcggc
     7381 accaggcgcc atcagtgatt gcgctttctg aagacgttca acataaccgt ccgcagggcc
     7441 tccgctggcc tggagcattc tgccttcagc catcaccagc aattgaatca gtctgccgtc
     7501 cacctcaccg gcaagttcca tggcttcgtc gataaagggc tgcacctcct gcagggtcaa
     7561 cccctcgcgc cagccgagca cgcagatctg gccgccggcc cgaacacgca ggttgtcggt
     7621 tctggcagac cggggttgct cctgcaggag cgcccgcacc ttgtgccaat gcttgatcgc
     7681 cagcgccgag ttggtggagc ccacccactg cgcggcacgt gcggcatggc tcgcagcctc
     7741 gaggaattgc cctgccgact cgtagtggta ggcaatcagg cccgcatact catcgagctg
     7801 atcgcggtaa tacacttcca tcgccaccgc caccgcgccg tggagcgggc cgcggcgcac
     7861 cttgagctgc gcgctgtagg ccacttcctg gatcaggggg tggcgaaagg tgaagcggcg
     7921 gccacccacg gcaggctgcg gcaggataag ttcagcgtga cacaggccat cgagcccttt
     7981 ttcgatcacc ttggccagcg ggctggccat gtgttccagc acggcgagcg ggatctcctt
     8041 gccgatgatg gcgcacatct gcagcagggt tttttcgggc tcgcccagac ggtcaaggcg
     8101 ggcgccaatc acagcttgca cggtagcggg caaggcccgc tctatcgcct ccagccctcc
     8161 ggcgcgaaga tcggagccct ccagaaaata gccgcggtcg accagcgaac gcaccagctc
     8221 ttctgcaaaa aaaggattgc cgccgcttcg cgctgccacc agttcacaga tgtcctggag
     8281 ttcggcacgg ggcccgatca gcaccctgac gagatgctct gtttccctga ggctcaattg
     8341 cccgaggtcg attttttcga agttttcaaa ctgggcccaa ggcaggcggt acgacggccg
     8401 gaaattgagg accaccatgg tctgggtgcc ggcggccgcc tcaaccagtg tcgcaacgaa
     8461 ctcttcgctg gcctcgtcca gccagtggag gtcttcgatg agaatcaccg acgtcgtggc
     8521 cgcatcatgc ttgaccagct cgcgcaccac attgagcaac ctggtgtggc gcgccttggg
     8581 actgagcgcg gacggcgcgc tgccggcctg cgccaccccg agaaattcgt acagcaaggc
     8641 caggtcggct tccgccgggg agccgagctc ggccatgcgc tcgacaatcc ggcttctggc
     8701 ggcggcggca tcgtcggcag gcagaatgcg gaaaaaga
//
LOCUS       DAIDFG010000112         8777 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_112_length_8777_cov_16.924903, whole genome shotgun sequence.
ACCESSION   DAIDFG010000112 DAIDFG010000000
VERSION     DAIDFG010000112.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 8777)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8777)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8777
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112_length_8777_cov_16.924903"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1552 a         2971 c         2783 g         1471 t
ORIGIN      
        1 aatgctgccg aacagatcgc cctcgacgcg gcgtgcctgc ggcagcaggg ccgcgatacg
       61 cgagcgcagc agcggcacgc tgctcgaacc accggtaaaa aacaccgtgt ccaccgcacc
      121 ggccggcacg ccggcatcac gcagcagacc gctcacgctg cgctccaccg tgccgaccag
      181 ctcgccgatg gcggtgtcga aatccgcgcg ctgcaacacc agtgtttcac cgggggcgat
      241 gcggcccagg tccatggacg ttcggggcgc gtccgacagc gcgatcttgg cttcctccac
      301 ctgcatcgcc agccagtgcc cggcgcgttc gctcaccagg ttttgcaggc gcgcgagttt
      361 gcccttctcc tgcgcctcgc ggtgcagctc gccgatctgc gaccccgcct gccgggtgta
      421 ggcgaagttg atggtgtgcc aggtcgccag gttgaagtag atggacgacg gcatcagcag
      481 gccattccgg agccggctgc ccaggcccag catgggcatc acgctggtca ggctcaggac
      541 cttgtcgaag tcggtaccgc ctatgtgcac gccgctgctg gccaggatgt cgtcacgccg
      601 atcggcccgg cccgcgcgtt cgggcgccag gcgcaccagc gcaaagtccg aggtgccgcc
      661 gccgatgtcc accaccagca ccagctcttc gcgcgtgacc tgcgactcgt agtcaaaggc
      721 cgcggcaatc ggttcgtact ggaaggcaat gtcgcgcaga cccacggcgc gcgcgatctc
      781 ggccagcgtg ttttcggcct gccggtcggc cgcggcgtcg ccgtcgacaa aaaaaaccgg
      841 ccgcccgaga acggcgcggg tgaagtcacg gcccgccgcg tgttctgcgc ggcgcttgag
      901 ttcgccgatg aactgcgcca gcagcgcacg aaacggcagt gcgcgaccgg ccacctcggt
      961 ctgctcgtcg atcagcgagg tgccgagcag gcttttgagc gaacgcatca gtcgcccttc
     1021 atcacccgcc aggtaatcgg ccagcgcggc gcggccgtaa ctgatgtgcc cgtcttcggc
     1081 gtgaaaaaac acgaccgatg gcagggtggc cttgccgtcc tccagcggca gcaggatcac
     1141 ctgccccggg ccagcaccgc cgcagcccgc ggtggagttg gaggttccga agtcgatacc
     1201 gcaggcatcg atcaatgcag gaaaagcgcc cgccatcagg gcttgaggaa gccgccgtcg
     1261 gcccaggccg tggcgctggc gcggctcagt ttgaaggccg gcgacacacg cacctgtgcg
     1321 agttgttcgc ctgcctcgtc atgcgcgctc agcatggcag acgggttgtc ttcatcgggc
     1381 acgatgtcca gtgacacggt gatacgcgca tcgcgcgagt ccacttccag gctctggtgc
     1441 agggtggcat gcagctgctg ctcatggcgg cgcacaaact ccgacgcggt cttgaccagg
     1501 gtgctgaagg ccgggccgtc gagcggcttg gggtttttct tgtcgcgccc catggtccag
     1561 gggcccacca gcgcgggctc gggttcgcca tccttgatca tggccaccgc ccagccgtcg
     1621 tcgtcttcgt tcttgagcac gcgggcggtc cagccgtcgc cgcaccacag gcgcggctgc
     1681 tggacagggg tgtcgggatc gggaagatgg tcggaggaag aagggtcgga cgggaagctg
     1741 gcggtatcgg tcacaggggg tcgtttcagt caaaaaaaaa gcgctgcggt gcgctgtctg
     1801 caggcgtcca ttttaccgcc tgcatcccgc agcccgaccc cgcgccggtc ggctgttctg
     1861 ccttgcagaa tacgctcccc gcgtaaacct tgatgacacg ccgacagcgg gccgctacgc
     1921 ttgaaacttc agatgttctg caacctccgg agacttatca atgaccaacc gccgcgcgtt
     1981 tattgccagc accacagcgg ccgtggccgg tgtggccctg cccaccctgg cccgtgcgca
     2041 ggcctatccc tcgcgcccca tcaaatacat ctgcccctgg ccggcaggcg gctccaccga
     2101 cgccgtcatc cgttcgctgg ccgaaagcgc cggcaagacc ctgggccaga ccatcattgt
     2161 cgaaaacaag ccgggcgccg gtggcatgct gggcgccatc gagatgctgg gcgccaaacc
     2221 cgacggctac acgctgacgc agataccgca cggcgttttc cgcattccgc acatgcagaa
     2281 agtcgcttac gacccgctca aggatttcac ctggatcgcc tgcctgaccg gctacacctt
     2341 tggcctggtc gtgcccgccg actcaccgat caaaagcatc aaggacctgg tggcctacgc
     2401 caaggccaac cccgaaaaat tcacctacgg ctcgaccggc accggcacca gtccgcacct
     2461 ggcggtcgag gagtttgcgc agcgcgcagg catcaagctc acccatgtgc cgttcaaggg
     2521 caacgccgac aacatgcagg ccatcctcgg cggccacgtg atgggtgcga gcgacgccac
     2581 cggctggggc ccgcacgtgg agtcgggcaa gcttcgcctg ctcgccacct acggcagcaa
     2641 acgtaccaag cgctggccca acgtgcccac gctgaccgaa ctcggctacg acaccgtgtc
     2701 ggactcgccc ttcggtgtgt gcggccccaa gggcatggac ccggccatcg tggcgacgct
     2761 gcacgaagcc ttccggaaaa cgttgaacga cccggccgtg ctggccacct tcgacaaata
     2821 cgaccagtcg gtgatctaca tgaacaccgc gcaatacacc aagttcgccc gcgaaacctt
     2881 tgtcgctgaa aaagcgacga tagagcggct gggcatgagc ttcaagggct gaccccggcc
     2941 ggcccgccgg cgcttaattg gcaggcaaac tgtcgcaagc cacgactggc aagcctttcg
     3001 tggctttttt gcgtgcttat ccacagattc gtgcacagga tgggtgggca acctgggtac
     3061 cgcattggcg ggagcacccg gcgcagggca tgcctgcggg taaaaatacc gcccttggag
     3121 aaagccacct cacctcaaga tttatggctt tatgttcact ttgtttcaat ttgccctgca
     3181 tcgagcatga accccttgtc cacagaaggc ttgcaacggc gagcccctgc ccagggccgg
     3241 cctgcgttat gctgaccatc tccctttttc cggtgccgac atgaccttcc aacctttcca
     3301 gatcctcccg gccatgcttg tgactgccac gctgctgttg accgcctgcc aagacacgcc
     3361 cgcgcccgac gcaaaggcca aagccggcac caccgctgtg tccaccgccg tcgtggccgc
     3421 cgaggccaag ggcttcagcg tcggctccac catgagcacc cgcacggttt atgttttttt
     3481 tgatccccag tgcccgcatt gcaacgcgtt gtgggtggcc gccaaaccgc tgaagtcgca
     3541 ggcgaaattt gtctggattc ccgtgggcat cctcaatgcg tccagcacgg cccagggcgc
     3601 aaccttgctg gccgcgaaag atccggctgc cgccatggat gaacacgaag cgtccatgca
     3661 agccaaaggc ggcggcatca gtgccggcag cgacatcgcg gcgcagaaag agatcgtggc
     3721 gaaaaacacc gcactgctga cccgcctcgg ttttggctcg atcccgacta tcatcggcac
     3781 ccacgcccaa agcggcgcgc tggtgacgca ggaaggctcc atgccgaccg cggccctggc
     3841 cgccctgctc gggctgcagg tgccggccgc gcagtaggca agctttttta taacaaaaag
     3901 tggctccagc ccaataacga cgggcgcaag cagctctctt attgatagct tctcgaccgc
     3961 tgcgcctaaa acagaacgcg gctacggatg gtgcccggca ccccggccag tttgaccagc
     4021 gccatgtccg aagaagcggc gtcgatgtcg atcaccacat aaccgatggc ttcgttggtc
     4081 tgcaggtact gcgacgcgat gttgatgttg ttgtccgaaa acacgcggtt gatctcggcc
     4141 agcacacccg gcacgttgcg gtggatgtgc agcagccggt gtttgccggg atgcgccggc
     4201 agcgccacct cggggaagtt gaccgaagag gtcgaggtgc cgttgtcgct gtacttgacc
     4261 agtttttccg ccacttcgac gccgatgttt tcctgggcct ccatggtgga gccgccgacg
     4321 tgcggcgtca ggatcacgtt gtccaggccg cgcagcggcg actcgaacac gtccttgttg
     4381 ctcttcggct cgacgggaaa cacgtcgatc gccgcaccga gcagtttttt ctggcgcagg
     4441 gcttgggcca gcggctcgat ctcgaccacg gtgccgcgcg aggcattgat cagcacgctg
     4501 ccgtgcttca tcgcggcaat ctcggccgca ccgatcatcc actgcgtggc ctgggtctcg
     4561 ggcacatgca ggctgaccac gtcggactgc gccagcagat ccgtgagctt gccggcctgg
     4621 cgcgcattgc ccagcggcag cttggtgacc acgtcgaaaa acaccacctg catgcccagc
     4681 gcctcggcca gcaccgacag ctgcgtgccg atggatccgt aaccgatgat gcccagcgtc
     4741 ttgccgcgga tctcgaaagc gttctcggcc gacttgagcc agccgccgcg gtgggcgacg
     4801 gcgcttttct cgggcacacc gcgcagcagc aggatggcct cggccagcac cagctcggcc
     4861 accgaacggg tgttggaaaa cggcgcgttg aacaccgcaa tgccgcgctc gcgcgccgca
     4921 ttcaggtcaa cctggttggt gcctatgcaa aagcagccga ccgccgccag tttgattgca
     4981 tgggcaaaca cgtcggcagt cagctgggtg cgcgaacgga tgccgacaaa atgcgcgtcg
     5041 gcgatcttgc gcttgagctc gtcgccttcc agggcccccg tgaggctttc gatctgcgtg
     5101 taacccgccg cctgcagcac cgccacggcc gagggatgga tgccctccag caggagaaac
     5161 ttgatctttt tcttgtcggt cgaggttttg ctcatggcgc tcaggcgggt tgtggagcac
     5221 cgaagtatga accaaggcct gcgtgcgagt cgcccggggc gcttttgccc gcaccgcaaa
     5281 aaggttgtcg caaccgcgcg gtcgcggttt gtcagtccac cggtgcgggc gcctcgcgcg
     5341 ccagcgcctg ccgccggctg gtgaactgtg ccagcgccac acccaccagt gtcagcgcgg
     5401 caccggccag cttgatgctc gtgtactgct cgccggcaaa cagccaggcc accaggccgg
     5461 cgaccggcgg catcaaatac atcagcggcg cggtgcgggc cacgccgcgc accgcgttga
     5521 cccagcccca gaccagccag cccagaaagg ccgacaccgt gaccgaccag accaggctgg
     5581 cccacaccgc cagcgaggcg tcgccccacg gcgccgacag gccggccggc agcgacagca
     5641 acaacaccgg cacgctgccg agcagggttg cataggccat ggtcacgacg ccgccatggc
     5701 gctcgatcag cggtttggcg gccaccgtgt accaggagaa aaaactcgcc gcgacaagca
     5761 ggaacagatc gccgccggtg gcacgccagt tgccgcccag cagtttgtcg gacaggaaaa
     5821 tcagcacgcc ggcaaacgcc acggccacgc cgacgatctg cgccttgtgc agtttctcca
     5881 cccccatgaa acgcaggatc agcagcgtga agatggggcc gcaggccagg atcagcgaac
     5941 tcgaaaacgc cgtggaccag tggatgccgt aagtcaccat gcccacgtgc aggaaatgcc
     6001 cggcaaaacc caggcgcgcg agctgccaga attcgcggcg cgtgatgcgc gggaaacgcg
     6061 tgccgaaccg ccaggccagc aaggccagtg cacacagcgg catgatgagg tagcgggcaa
     6121 acaggaagcc gccggggccg accacgttga agacaaactt ctgcagcgaa aagttgatgc
     6181 cccagatcac caccagcacc agcgcagcgg ccagggcgat ggacgcacgg cggagttttt
     6241 tctgttcggg ggtcatcgcg ccgtgttccg tgaaagtttg gggtcaaaca ggcctgtagc
     6301 ccaatggatg cgggcgcaag cagctatcaa tttggaagca caccagcttc cggccaaagc
     6361 tgcagggccg ccgccccggc gccggacggg ggcttcagaa gttgctgccg accagcgcct
     6421 ccagccgggc cggatctacc ggcttggtga aatggtggtc aaagcccgcc tcctgcgcct
     6481 tgcgtttgtc ttcggcctgg ccccagccgg tcagggcgac gatgaccggg gcaggcgcct
     6541 ggcggagcgc gcgaatcctg cggcaggctt cgtagccgtc catgcgcggc atgccgatgt
     6601 ccagaaaaat gatgtctggc ctgaaggtcg cggccgcctc gatggcctcg gcgccgtcat
     6661 gggcggtgcg cacctcgtgc cccaacatgc ccagcaggct cgccagcgtc actgcagcat
     6721 cgacattgtc gtcggtgacc agaatgcggc gcggctcgaa aacgcctgcg gggtgccgga
     6781 ccggcgccag cgccgcagct tgggccgtgc ctgactctgc cgcggccggc gtggctgcgg
     6841 tgtcggccat gggcagccag acgatgaagc ggctgccggg gccttcactg cggctttcgg
     6901 cccgaatgct gccgccgtgc atctcgacaa tcttgcgcac cagcgtcagg ccgatgccca
     6961 gcccgccctg ggcctgcggc gtggccgggc tgggctcgcc ctgaacgaac atgtcaaaaa
     7021 tgcgcgtgct caggtgctca ggcaccccga tgccgttgtc ctcgatgctg accgtcacgc
     7081 cctgcgcgtc ggcgcttacg tgcaactcca cggcgccgcc ttcgggcgtg tatttggcgg
     7141 cgttgttgag caggttggtg aaggcctgcg acaggcgcac caggtcggcc gtcagcacaa
     7201 tcggctggtc cggcatggtg acggtgaaca cgtgctgccg ctcgaggagg accggccggc
     7261 tggtctccgc cgcactgctg atggcgtccg cgagccggat cggctcgagg tgcagcccca
     7321 gcttgccgcg gctgatgcgg ctgacatcga gcaggtcgtc gaccaggcgt gtcaggtgcg
     7381 cgatctggcg ctccatgatg gcccgggtgc gctccaggac agccacgtcg tccgctccca
     7441 gccgcaggat ttccgcgctg gcgcgcagcg gtgccaaggg gttgcgcaac tcatgggcca
     7501 gcatggcgag gaactcgtcc ttcatgcggc tcatctgttc ggcctcgctg cgcgcggcgc
     7561 gctcgttgtc gagcagcttc tcgcgttcct gcgcggcctg cctggccact tcatacagcc
     7621 gggcgttgtc gatggacacg ccggcctgcg ccgcaatgcc agtcaccagc ctttcggcac
     7681 gttcgtcgaa aacaccggca tccggatggc cgaaaaacag gccccctatc acctccgcag
     7741 aacccgacgc cacaggcacg gccaggtaac tgcggatcgg caccgtcccc ggctgggccg
     7801 cggcctcata gcgcgcgtcc ttcgtcagat cgtcgagatg gaccagcggc gcgccgctga
     7861 gcgtgaggtc gaacagtgca ccggcctgcg gcaggccgca gctgtcgaag tcctgctcgg
     7921 cgatgccggc caccgttcgc agaaaagggc tgtcgccgct ccgggccgcg gtgttgtaga
     7981 aaaagacacc tactttggcg ccgctgatct ccgtggccgc gtcaatgacc gcctgtgcca
     8041 gcatgcgcac gtcgagcttg gccgtcagga tggtgccggt cttgttgagc agttccagca
     8101 cccgggcctt cttgcgcagg gcatcctcgc tgcgccgctg ttccgtgatg tcgcggaaaa
     8161 cccagacccg cccgacatgc tggccttcca cgatctggat cttggaagaa cgttcgtaga
     8221 cccggccatc gcgcagctgg agcaggtcaa aactttccgc cggcgagtgg cggtagatct
     8281 catggacccg gtccagatag tgctgcgcat cgtcgacctg ccggctcgcc acggccagca
     8341 gccgttcgtg gtcgcgaaac gccatgagtt catcggacag cccccacatc cggacgtaca
     8401 gctggttgaa gcccgtgacg tgggcgttgt catcggtgac caggatgccg tcgcggctgg
     8461 cctccagggt ggcccgaacc atggacagcg acagcgccag ctccgtcgtt ttctgctcca
     8521 gcccgtccct ggcggccacc agcacacctt cggcctgctg gcgcgcccgc agaatgctct
     8581 gcgcgttctg cagggcaacc gagcgcagca ggcggtcttc gtcgtcggag gcctgcatca
     8641 tcgtccttct tcaggcatct ctaccccggg ccggattggt gacagcacgc cggcgaacac
     8701 acgtgggtcc ggtttgaggc gcaagcgccg tgaatgactg ccaatcggcg gggtcggaat
     8761 atgcatggtg ctttact
//
LOCUS       DAIDFG010000113         8673 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_113_length_8673_cov_18.297981, whole genome shotgun sequence.
ACCESSION   DAIDFG010000113 DAIDFG010000000
VERSION     DAIDFG010000113.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8673)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8673
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113_length_8673_cov_18.297981"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1668 a         2679 c         2787 g         1539 t
ORIGIN      
        1 gaatagggcg cacttttcgt gtggaagtcg gcggcaaggt gcatgccggc tggattcacg
       61 ccagcttcga cgaaagccgc tgccacgtcg cgctgcgcat cagcgactcg ctgcgcgagg
      121 tgttgccgct ggtgatacgg cggctgcgcg ccatgctgga cctcgacgcc gacccgcagg
      181 ccatcaacag cgtgctgcac gccgcttttc cgggcggcga cggcctgcgc gtgcccggtg
      241 cgatggacgg ttatgaactc gcggtgcgcg cggtgctggg ccagcagatc acggtggcgg
      301 ccgcgcgcac gctcgcgcag cgcatggtgg acaagttcgg cgagccgata gaaacgccct
      361 ggcccgccct gacgcggctt tttcctgcgg cggcggtgct ggcgcaggcc agcggcgacg
      421 cgctgggcca gctcggcatt gtcaggcagc ggcagaccgc catcgtcggc attgcccagg
      481 ccgtggccga aaagcgcctg caactgcatg gcggcgccga tgtcaacgcc accatcgccg
      541 ccttgaaagc cctgcgcggc attggcgact ggacggcgga gtacatcgcg atgcgggcac
      601 tgcgctggcc cgacgccttc ccgtccggcg acgtggcgct gcacaaggcg ctcggtgtgc
      661 aggggctgaa aaacccggcc aaggaagcca ggctcgcctc gctggcctgg aagccctggc
      721 gaagttatgc cgtcatccgg gcctggagcg gggcgatgca gcccagcggc tgacggcgcc
      781 tgggatccat gcgttcaggg aatgaatcag gtgcctcgat ttcattggac gaaaatgcgc
      841 cgtcgcgcga ggatccatgg cgaatgatcg aggttctgtt tttcctgaat gcccgcaagg
      901 cgaacgacgt ctgagaagaa agttgttttg ctactgaatt tatagctgga cacgcccgcg
      961 cttaaagggg aaaaacgcaa taacagcctg aatgacaggc agaacccgaa accattcaaa
     1021 ggtgaacatc atgaaattta cccgctccgt cgttcaaacc actttccaga gccctctggg
     1081 caagatgatc attgccgcaa ccgacaaggg cctggccggg ctctggttcg agggccagcg
     1141 ccacctgccg ccggaactgg tggaccctcc ggtctggccc agcgatccgg cacatcccgt
     1201 tctccagcag gtgatgaccc aactgaccga atactttacg ggacagcgca cgcaattcga
     1261 tgtgccactg gacctggcct atggcacggc tttccagcaa tccgtgtggc aggcgctgct
     1321 gaaaatcccg caaggcggca ccgccagtta cggcgaagtg ggccggcgca tcggcaaacc
     1381 ggctgccgtg cgcgccgtgg gcgccgccgt gggccgcaac ccggtcagca tcatcgtgcc
     1441 ctgccatcgc gtgatgggtg ccaacggcgc gctgaccggt tacgccggcg ggctggaccg
     1501 caaaaccgcc ctgctcaagc tcgaaggcgt tcttcaggag caccggtctt gagttcaaca
     1561 tccgcaagtg caagtgcaag cgccggcgcc cgcccgaaac actgggccat cgacttcgtg
     1621 ctgctggccg ccatctgggg ctcgtccttc ctgttcatgc gcataggcac ggtcgagttc
     1681 ggcccgttgc ccaccgccgc cgtgcgcgtc gctattgcgg cggccttttt gctgccgctg
     1741 gtgctgctgc gcggtctcgg cacgaccctg gtgaaaaact ggcgccatgt gttcctgatc
     1801 ggcatgttca attccggcat tccgtttgcc tgcttttcgt ttgcgctgct gtcgatcacg
     1861 accggactgt cggccatcct caatgccacc gtgcccatgt tcggcgcact cgtggcctgg
     1921 ctctggctca aggacaagcc caaccactcg cgcctgctgg gcctggtggt cggttttgcc
     1981 ggggtcgcca tgctggcctg ggacaaggcc accttcaagc ccgacgcctc gggcgtggca
     2041 ccgggctggg ccgtgctggc ctgcctgttt gcctgcattt gttatgcgct gtcagccagc
     2101 tacaccaagc gttacctgac cgggctgccg ccgctggtca ccgccgcggg cagccagatc
     2161 ggcgccaccc tgggcctggc gctgccggcc ctgtggctgt ggccggcaaa gatgcccggc
     2221 agcagcgcct ggctggccct gctggtggtt ggcgtgttat gcaccggcat tgcctacatc
     2281 ctgtacttcc ggctgattgc atccgccggc ccggcgcgcg cgctggccgt gacgtttgtg
     2341 gtgccggtgt ttgccgtgtt ttacggcgtg ctgtttctcg gcgaggccgt cacgctctgg
     2401 atgctgctgt gcgcggtggt tatcgtctgc ggtacggcgc tgtccaccgg gttgcttaaa
     2461 atcgggcgat gagttctgca cgcaaaacca agatcaagct caagatcaag gtcacccggc
     2521 cgcgcaaccc gctggtcgtg ccggccctgc agcgcaaggc gggagcgcac cgcaagtcgc
     2581 gctcggccgc ccggcacgct gacaaaagag ccctcgacaa gcagttgcgc gaagaatgac
     2641 cggcaggccc tggtgcgggg tcagtgcagg gagcccgcca ccacattgat gctgagcgcc
     2701 aggatcagca tgttgaaggc aaaggacaac acggcgtgga ccgtcgtgag ccggcgcatc
     2761 gcgcgcgagg tcacctgcac gtcggacacc tgcgaggtca tgccgaccac atgtgcgtaa
     2821 tacagaaaat caaaatagtc ggggtcctgc ccaccaggaa acagcaggcc ggcacccgct
     2881 ggctgcttgc gtttttcttc ctggtaatag cggtgcgcgt aatgaaacgc aaagatggcc
     2941 tggatgaaca gccaggacgc cgccagcgcc gccatcgaca gggcaatctg cagcgtgcgt
     3001 gccacgcccg agagctcccg gctctgctgc atcatgaaca caatggctgc cacgcaggtc
     3061 agggtggcca gcagcagcat caggaaaatc accacgccgg gctcgtcctg cgtctgggcg
     3121 cgttcacggg tgcctttcgc gtcaaaagcc atggccagcc accaggccag caagaggtag
     3181 acggccacgc ccacacacca gccgagcaag ccgcgcaagg gccaggccag cggcagcggc
     3241 agcgctgccg tgaccaggcc cgccgcgagg ccatacatca ggcgctgcgg acccgtggtt
     3301 tcggaaaagt gtttgcgcat gcgcccattg tgatgccagc gacaagcggc ctcaaggctt
     3361 gagggacgcg gctcaccggc gcgttgcggg agaaagcagg ggctggccgc aagggccgga
     3421 aggccggcga tctatttctt cttcagccct tcaatcacca gcgccgcctc gatgtcggcc
     3481 tcctgcagct tcaggttctg taccaccagg tcaaaggcag cgcccatcat gatggcgcgg
     3541 gtgcgttcgg ggatctgttc ccagttctcg gccagctcgt tgatcagcac ttcggccagt
     3601 gtgcccatgt caaacacccg cccctcggac aggtccgcct ggtccatccg ctcatggagc
     3661 agacgcatga caaaatcccg gttcattttt tctccctgat ggacgattcg gcgaaaagat
     3721 cggccgcctg tgtattttcg gcaataacct gacgaaggtt accgcgtccg cgggcgccct
     3781 gcgcgcggca acctggaaaa cgccgacaat gacaggtcac aaggccgggc gacgactcca
     3841 ccccggccgg tcatctccac cggatcacac atttccatgg aatttttcaa gccatccgcc
     3901 ctctgggtcg tggacttcca ctacgaaggc cgtgcgcgcc gctggttcaa ggccttcggc
     3961 cccaaggacg atccgttcca gaaaatgacg gacaccctgc aagacctgta cggcgaccgc
     4021 gccaaactgg cgacggtgcg caaggcaacc gaggaagagg aattgcagta cctgcgcggc
     4081 gaagaaccga aaaacagcta ctgcccgacg gggcgctgac cgggcgctaa acggggaaat
     4141 tcatgcggca tggcccgccg cgccatggcg cggcttcaca cctgtctgcg cgcgcggccg
     4201 catgaaattt cagggggtca ttcgccgagc cagaaccgtt cgctgctgtc caggaaatcg
     4261 tgcacgtgga ccgtcacaga ctcctcctgg aggaggacca cgctgtactg cggtggctca
     4321 tggctgcctg gaatattcgt tgtttcatcc agcctcaagg ccacctggtg attggtgccc
     4381 cgcatggtcg aaaacgggat gccgcgccag ctgccgaaaa taggccgatg aatgtggccg
     4441 aagaacaaat gcctgatgcg ctgggtatgc ccttgcagcg ccgcctgaaa aggcccggtc
     4501 tccagcaagg caatgcggtc catggaggga atgcccaggg caaacgccgg gtggtgcatg
     4561 aacagcaaca ccggccggtc atcctccgcc agccggtcgg ccagccattg ggcgcgtttt
     4621 tcgcacaaca cgccgtaact cacgccgggt tcgttcgtgt ccaggaagat gccgcggtac
     4681 tcgccgatgg gcgactcata ctggacaaaa ccgttcgcat cagtctgtac ctgcggaaaa
     4741 aattcccgga aattggcgcg gttgtcatgg ttgcccagga tgggcaggac aggcatcggc
     4801 agccgggcca actgctcgga cagctggcgg taggcgtccc tatggcccac atgcgccagg
     4861 tcgcccgtga ccacgcacaa gctggcgtcg gagtggtcct gaatgacact gtccaggcaa
     4921 cgcgccagtc gcttgcaggg gtccagccca tagagggcat ggccctgctc gacgaggtgg
     4981 gtatcggtga tgtgaatgaa cttcagcacg acagctcctg atcagcgtgg cagcaaggcc
     5041 tgcacagcct tgctcatgtc ggccagcgcg gcctcgggct gggccgcttt cgacaccacg
     5101 gtctgcagcc gatccttgat gacgtccgtg atcttgaggc cgttttcgcc ggggaaagcg
     5161 taccagcccg tcaggacggg caactgcttg acggccgtga ggtagttcgg gttttgctca
     5221 taaaacggct tgagcatggt ggcgtcgttc gccggaatca ggttggctgg gaaataaccc
     5281 gttcccttca ccatcgtcgt ggcgcccagg gggcccgtgg tgaatttgat gtattcccac
     5341 gcggctttct gtttggccgg gtccttggtg aacatcatcg cgacattccc gccggcaggc
     5401 aggcgtccgg tgccagtcgg gatggggaaa cgcgccgtgc gcacttcgaa gcgcttgccg
     5461 acttccttca tcacggtacc caggttggac gtggagtgcg accagatggc caggttgccg
     5521 gacacgaagt cctgccgcga ggtggccgac gacacgtcgc gcatggtgcc ctcgttgacc
     5581 atcttcgcca gggtctggat ggatgccttg cccggcgcgc cgtcgaaggc caccttcttt
     5641 tcgtcggcgc tcagcatcgt gccgccgttc gaaaacacca gcgcttgcca catccagttg
     5701 ccggtgatgt cccagtcgaa gtggaacccg tgcgtgccgg cgccactggc cttggccttg
     5761 cgcgccagct cgaagatgcc gtcccaggtg gtggggaact ggtcgacgtt accgccggcc
     5821 ttgcgaatca ggtcggcgtt gaaataaatg atgggcgtgg acagcgcaaa gccgatgccg
     5881 acctgcttgc cgttgacctg acccagggag agcagcgcct tgtcatagcc catttgggac
     5941 caaccttttt ccgcctggat gaagggcgtc atgtccacgg cgatgccacg atccgcgaag
     6001 atgcgctggc ggttcagccc ctggtaggac acgtcggcca gctggttggt gaccgcctgg
     6061 cgcagggact gctgtgttgc ttcctcgtag cccggggtcg gcgcgcggaa gctgaccttg
     6121 tactgtggga atttcgccat gaagtcctgg gcgactttct cgtggacttc cttgaaaagc
     6181 tggggaaatg ggtagtcgac cacgatctca accggctgct gggcttgcac cacgccacag
     6241 gtcagcagcg cagcggcggc gatggatttc agaaaaatgc gtttcatggg gtttactcct
     6301 aaattgatga actaaccttt gacgccagtg agcgtcacac cttcaataaa gcgcttttgc
     6361 gctgcgataa aaaacagaac cagcggcgcg gtgatcagca ccgtgcccgc catcaggggg
     6421 ccgtagtcgg tgccggcctc ctcgttgcga aagaacatgg tcccgagggg cggcgtggcg
     6481 agttcgggcg aagcgatgac cagcagcggc cagaagtagt cattccagtg ccacaccacg
     6541 gaaaatatgg agaaggcaaa cagggccgga atcgcggtcg gcagcatcac ccgccagacc
     6601 agttccagct cgctcatgcc gtccaggcgc gcggcgtgaa tcaggtcgtc gggcaccgta
     6661 cggaagaatt gccgcatcag gaagatgcca aaagccgaga tgatgaacgg cacaatcagg
     6721 gccgacaggg tgttgagcag gcccaggtga tgcagcatca cgtagatggg aatggccgtg
     6781 gcctgatggg gaatcatcag cgccagcagc accagccgcc aggcccaggt cttgccccgg
     6841 aagtcgagct tggcaaaggc gtaagccgcc ggcagcgcca ccacgatctg caggaccaga
     6901 atggccgcgc acacgacaat gccgttccag agataacgca gcaggggcac cttggtcagc
     6961 gcctgtgtgt agttttcaac caatgccagc ctgcttggca acagggagaa ttcccgcgtg
     7021 aaaatctcgt cggccggttt cagggaggtc agcaccatcc agatgaacgg ggcaaggaag
     7081 acggcggcac cggtcatcaa caccagatgg cgaaagctgc gaaagaggat ggatggacgt
     7141 ttcatcagta gtgggtcttt cgttccatgg ccaggtgctg cagctgggtg agaaagaaga
     7201 tgcctgcaat gaacaccacc gtcatcgcac tggcataacc catgcggaga aaactgaagc
     7261 cttccgaata gatggtgtgg agcagaactt cggtggcctt gttggggccg cccttggtca
     7321 gcacatgcac ggtgtcgaac acctgcaggg cccggatggc gcagacggtg acgacaaaaa
     7381 gcgtggtggg ccccagcagc ggccaggtga tgcgccggaa gcggtccagg ggatgttcga
     7441 tgccgtccag ggcgccggcg tcgcgaatgt cctggggaat gctcttgagc cctgccagga
     7501 agaggacaag ggccagtccg gacatctgcc agatgccaat cgcggcgagc gagtaaagcg
     7561 cggaccggcc gtcattgagc cagttgcgtg gctccatgcc cagcaatcca agcacctgat
     7621 tgaccaggcc cacactgggg tgcagcagaa attgccagac caccgccatg gcgatcaggg
     7681 tggaggccac cggcatgaaa aatgccgtcc tgtagaaggt gcggagctgg gtgtccgact
     7741 caatcaacag agccgccccc aggccgatgg tgatcgagcc caatgtcacc acgaccaggt
     7801 acgtgagtgt gttggaaatc gatttctgaa agaccgggtc ggccaccagc tcgcggtagt
     7861 tcgccagccc gacccaatcc agggtcgcgg cgccgaattg ccagttggtc agggacatga
     7921 ccgccacgat ggcaacgggc accacaagca gcatcaggat gagtaccgtc gctggcgtaa
     7981 gcaacagggc ggtgtgtagc ggattgactc gcatggtccg gcctcaggcg gctttgctca
     8041 tgacaggcgg gagggcttca aacggctggc gctggccatc ggcatcgaac agatggattt
     8101 caccaatggg caccgaaacc cgaagcgcct ggcccacccg gatcgccggg ctgttggcgg
     8161 ggaaacagaa tacgcaggcg tcttcgatgc catcgacctg gagcgatgca atcagttcgg
     8221 cgccggtgta ctccacattc accagggtgc cttcaacttc cagtccgtcc acggactcca
     8281 gcgtcccggc ctggggcctg aacgcaatgc ggcaggggcc ggtcttgccg tgggagtgac
     8341 caaccagccg gccttgcgcc cacacgacac catgcgcttt gacttcgccg tcgagcaggt
     8401 tgatgcgcgg cgcgcccacg aagcgggcaa cgtcgaggtt ggcgggccgt gtatagattt
     8461 cctcgggtgt tccgagttgc agaatgcgcc cttcacgcat caccgcgaca cggtccgaca
     8521 tggacatggc ttcatgctgg tcgtgggtga cgtagatgaa ggtcacgccc aactggtcat
     8581 gaagctgacg gatttccccg cgaagtgcct ggcgcaggtt ggcgtcgagg ttggacagcg
     8641 gttcgtcaaa aaggaaaacg gccgggcggc gca
//
LOCUS       DAIDFG010000114         8668 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_114_length_8668_cov_18.538837, whole genome shotgun sequence.
ACCESSION   DAIDFG010000114 DAIDFG010000000
VERSION     DAIDFG010000114.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 8668)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8668)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8668
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114_length_8668_cov_18.538837"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1470 a         2813 c         2900 g         1485 t
ORIGIN      
        1 cgcaagccca tgtcgaagct gaaggggccg aaattgacca cttcctgctc gctcgatggt
       61 gcgcccggca cttcgaccgc ggggcggcgg cgcagcacgg catggatgcg cgcgagcagt
      121 tcgcgcgggt tgaaaggctt ggccaggtag tcgtcggcgc cgacttccag gccgacgatg
      181 cggtccacgt cctcgccctt ggccgtcagc atgatgatgg gggtgcggtc gttggcggcg
      241 cgcaggcgcc ggcagatgga caggccgtct tcaccgggca tcatcaggtc gagcacgatc
      301 aggtccacgg cgtcgcgcat catgatgcgg ttcagggcct tgctgtcctc ggccaggatg
      361 acttcaaagc cttcctgtgt caggtagcgg cgcagcaggt cgcggatgcg ggcatcgtcg
      421 tcaagaatca ggatcttgtc agcgcgggtt gaattgctag cttgtgtcat aaggcgatcg
      481 taatttgtaa cagagccaat tctgcgggct aaaaacgcgg attcgagatt tttcgtgcac
      541 tttgacagac tgttacagaa gttgcagttg cgggcaagcg cgataaggga gagatgcgaa
      601 ccaatggcac gcttgggtct ttggatggca accattggcg gcagatgctg acaagccgga
      661 cctcaaaaac cacttatcgt ttcgccggtg gcgcggcttg tttcgcgtgc cagctgcttt
      721 ttaattcaac tgaaagtgat cccttgaaaa aaaccgccca actctttgcc gcctgcgcgc
      781 tcctggccgc aagcacaggc ctgtttgccc aaaaccagcc tgcaccttat gcgccgctgc
      841 aaaacgttgc tcagctgtcg gccagcgcgg ccgtcgaagt gcagcaggac ctgttgtcca
      901 tcgcaatgac gaccacccgg gacggcagcg atgccgccac ggtgcaaaac cagctcaagc
      961 tggccctgga ggcggcgctg gccgaggcca gaaaaaccgc ccagcctggc ctgatggatg
     1021 tgcgcaccgg caacttcagc ctgtacccgc gttatggcaa ggacggcaag atcaatggct
     1081 ggcagggcag cacggagatg gtgctggagg gccgggattt tgcgcgtatc acggcggcgg
     1141 ccggcaaggt ccagaccctg agcctgggca atgtcggttt tgcgctgagc cgcgaggccc
     1201 gtgcccgtgt cgagggggat gcgcaggcgc aggcgatcga gcgtttcaag gccaaggccg
     1261 gcgagattgc gcggggtttc ggtttttccg gctacacgct gcgtgaagtt tccatcaatg
     1321 ccaatgacca gggccccatc cgtccgcgcg tgatggccat ggaagccaag tccgcgatgt
     1381 cggactcggc gattccggtc gaagccggca aaagcaccgt gctggtcaat gtgtcgggct
     1441 ccgtgcagat gaagtgatga cctgccctcc ggcgggcggc ccgttcggcc gtaccggaca
     1501 cttccggaag ggtgtttgcc tgacagacgc aaaccgggcc ggtccgtaag ctggtgggca
     1561 tgaatgttca ctttctggcg caggacgccg cgcgccgggg cccgctgttg atagtcatca
     1621 atgccggctc gggccaccac aacaccgacg atgaacaggc cgtgatggcc cgcgtattca
     1681 cgcaggccgg ccgcgagttc gagttcctgc cgattgatgc agccgaccag atcgacgagg
     1741 tggcgcgccg cgctgtccgc caggccaagg cccggggcgg cgtggtggtg gctgcgggcg
     1801 gggacggcac catcaacgcc gtggcccgcg ccgtgctggg cagcggttgc cccttcggcg
     1861 tcctgccgca gggcaccttc aattatttcg gacgcgtcca tgcgatcccg caggaaaccg
     1921 aggcggcagc cagggccctg gtcggcgcca gcgtgtcgcc ggtccaggtc ggccaggtca
     1981 atggcaggct gtttctggtc aatgccagtc tgggcctgta cccgcaattg ctggaggaca
     2041 gggaagcctg gaagcagcag ttcggccgca gccgtctggt ggcttttgtt tcgggcatgg
     2101 cctcgctgct gcagacgcgc cgccagcttc agctgcagat cgaatcggcc ggccaggtgg
     2161 ccgccctgcg cacaccgacg ctgtttgtcg gcaacaacca cctgcaactc actcgtgtgg
     2221 gcattgacga aaaagacgcc aatgccgtgg tgcatggcca actggccggg gtggtggtgc
     2281 gtcccatcgg cacgctggcg ctgttcggcc tgctgctgcg cggcctgctg gggcggctcg
     2341 gcgatgcgga caacatccac agcttttcgt ttcgccgcct gaccgtcacc ccgcgcgggc
     2401 gccggcgcgt caaggtggcc accgatgggg aagtcagctg gatggaggca ccgctggtgt
     2461 tcgaggtggc cgaccaaccc ctgttgctga tggttcccgc gccggctgac cgggtggaag
     2521 tggcctgatg aacaagctgc tgcagatctc cgacccccat ttcggcacgg cgcagccgcc
     2581 ggtgatggac gcgctggtgg agctggcgcg gcagcagcgc cccgacgtgc tggtgctgtc
     2641 gggcgacatc acgcaacgtg cgacggcggc gcagtttggc gcggcgaggg cgttttgtga
     2701 acgcctgcag attccccaga tgctggcctt gcccggcaac cacgacatcc cgctgttcaa
     2761 cccctggcag cggctggtca cgccttatgc gcgttacctg caggacttcg ggcccggcct
     2821 ggagccggtg ctggacaccg cctggctgca actgagcacg gtgaagacca cacgccgctg
     2881 gcgacacaaa aatggcgagg tgtcggccag ccagatcgaa cgggtggccg cgcgcctgcg
     2941 ccaggccggg cccgggcagt tgcgcgtggt ggtggtgcac cagccggtgt atgtgctgcg
     3001 tgctgaggac gagcacgacc gcctgcgcgg ctgggagccg gcggtgcgcg cctgggccgc
     3061 tgccggcgcg gacatcgtca tgggcggcca catccacctg ccttatgtct gcgaactgtc
     3121 ctcctgcgtg gacggcctgc aacggcgcct ctggtgtgtg caggccggta ccgcgctgtc
     3181 ctcgcgcata cggcgcgagg cgccgaattc cgtgaacctg ctgctgtgcg atcccgccga
     3241 ccatccgctg cggggtcggc tcgaacgctg ggattaccgc agcgccggtg gcgcggggcg
     3301 ttttgaagtg gcgcacacca gcgagctggt gccggaccgc gcagcctgac cggccgggcg
     3361 catcaggcgg cgggcagcgc tttttttcgc aggtggcggg ccacgacgat gtcgcgcagg
     3421 tggtcgtagg tccagttgaa ggcaaaggtg tagggcagaa aaaacagcac gatgccgata
     3481 tccagcacaa aggcctgcca tagcccgatg tccagccacc aggcggctag cgggaccacg
     3541 gccaggatca ggccgacctc gaacagcacc gaatgcacgg cccgcgcggc cagcgtgcga
     3601 gggaaaccca ggcggcgctg ggcacggtca aacaccgcgt tgaacaccat gttccaggcc
     3661 atggcgatca gcgaaatcat cagcgtcagc agccccatgt gggccagcga gtagccgagc
     3721 agccaggcgc ccagcggcgc gcagagggcg atggccagca cctcgaaacc gagggcgtga
     3781 aaaaaacgtt ccttgacgga cttctggagg ctggggttca tgacggagtg aggggaatgg
     3841 gggagtggaa atggcaagga ctcgattttt atcgctattt ccaatacatt aaagttttct
     3901 aatatctaca aaacagatag ataaccgcca tgcgccattc ccccgaagcc ctgaccgcct
     3961 ttgccgaagc cgccgcactg ggctctttct cggccgctgc gcgcaagctc ggcaaaagcc
     4021 agtccacggt gagcacggcg attgccaacc tcgagatcga cctgggcctc tccctgttcg
     4081 accgcagcag ccgcaaaccc gcgctgaccg aacacggccg ggtgatgctg ggcaaggtgc
     4141 aggagatcct ggccgccagc gaccggctcg acctgtcggc cagccagctg ggcggcggcc
     4201 tcgaagccag gctcagcgtg gtctggtccg acacctacca gtcggaccgc ttcgaggaca
     4261 tgctgaccgc cttcgagcaa cgttttccgg acctggagtt tgagtgcctg attgccgaac
     4321 acggcgacct ggtgtcgctg gtgcagaccg ggcgtgccca catcggcgtg gtcggcgcgc
     4381 agggggcgta cccgcctgac atcggttcgg ccactattgc cgagcagtcc gagatcgcgc
     4441 tgtttgtcgc caccagccat gcgctggccg gcaaaacgga catcacgccc gagctgctgg
     4501 cccggcaccg cgagttgcgg ctggacacct acctggggcg ggctgaccag gtcatgccgg
     4561 cggcgcgccg ttcctggtcg gcgcccagct acctgatgct gctggaaatg gcggtgctgg
     4621 gttttggctg ggccgcgatt ccgcgctgga tggtcacgcg ttttgccgtc ggcaaactca
     4681 ccgagctggc cgcgcgcggc tggccgcaac gggtgccggt ggacgccgtc tggtcgcgcc
     4741 agcggccgct gggccgcgcc ggcacctggt tgctgcagga aatgctggcg ccaccggtgc
     4801 cgcctctttt acaagcaaaa tgaggctcca gcccttatgg ggcgggcgat ggcagctctt
     4861 tatttgatag caagcggggt tcggccgcgc tggcgggcgt cgccttgccg acttcgccca
     4921 gcagccactg acggaaggcg cccagcggct tgaggtggct gcgcgactcg gggtaacaca
     4981 ggtagtaacc catgtcgtcc ctgtagccgt cgtccagcgg cgtggccacc aggccggcgg
     5041 caatgtcgtc ctgcaccagg caatgcggca ccagcgccag acccatgccg gcccgcaccg
     5101 cgcgtatcag gctgtggtac tggtccagct gcgggcccat cagcgtgttg acgccgctga
     5161 cgccgtgtgc tgcgcaccag tgggtccacg cgtgcggcac gctcacatgc tggagcaggg
     5221 tgaagccggt gatgtcctgg gcctggcgca gcggcggttg cgccggcagc cccggcgggg
     5281 cgatcaacac gacttcacgg ccggtcaggt agtcggcccg ggcgccgggc caactgccgt
     5341 tgccgaacag aatcgcgcag tctaggtctt cgcgctggaa gtcgtagccc tgggcatagg
     5401 gcacatagtg cagctcgatc tgcggatgct ggcgctggaa ggccggcagg cggggtatca
     5461 gccatttggc gccgaaggtg ggcagggtgg acaggtgcag ggcgccgccg ccgtcgccgc
     5521 tggtgatcag ctccagcgtg gcggcttcga gctgcgccag caccggccgc acggcggcct
     5581 cgtaacgcac tccggccggg gtcagcgtca ggcgctggcg gatgcgctcg aacagcagca
     5641 cgccggtcag ctgctcgagc tcgctgatct gtttgctgac ggcgctttgc gacaggtgca
     5701 aggcttcgct ggcgcgcgag acgctaccga aacgcgcggt ggtggtgaag gcgcgaagca
     5761 ggtgaatcgg cggctggagg cgtttcatgg cccgatttta aatcaagttc attccaatat
     5821 ggacttaacc aagtcgaaga cttcaccggt ggctccggcc gtctggcacc actcctgcta
     5881 cacaatggat tcacaaatcc attttgaaaa ggttcaccat gcaaagacgt cacctcatcg
     5941 cactcgccgc tacttcagta ctcccgggaa tgggttgggc gcagtcgaac aaaccgctgc
     6001 gcctggtggt gccgtttcct ccgggcggcg cgacggacat caccgcccgt gtgttgtccg
     6061 aaccgctgtc gcgcgttctg ggtcagccgg tcatcgttga gaatcgcggc ggtgccggcg
     6121 gctccatcgg catgagcgag gtggccaagg ccgcgccaga cgggctgaca ctcggtgtgg
     6181 cgaccctgtc cacgcacggc gtcaatccgg ctgtctacaa gaagctgccc tacgacccga
     6241 tcaaggattt cgtcgccgtc accgagctcg tcaaggcgcc cggcgtggtg gtggtcaatg
     6301 ccgccctgcc ggtgaagaac tttgccgaac tggtcgccta cctcaaggcc aacccgggca
     6361 agctcagcta cgcgtcgccc ggcaacggca ccatcggcca catgtggggc gagctgttca
     6421 aaagcacgac caacaccttc atggtgcaca ttccctaccg cggtgcgggc ccggccctca
     6481 acgacgtcgt cggcggacag gtgatggtgt atttcgacca ggtggccgct tcgctacctt
     6541 tcatccagtc gggcaagttg cgcgccattg cggtgtcctg ggacaaacgc ctggacgtgc
     6601 tgcccaacac gccgacctat gcggagctct cgctgttttc cagcaacgac ccgtcctggt
     6661 tcggcctggt tgcaccggca ggtacgccgg cggcggcggt gaaccgcatc cagcaggccg
     6721 tggtcaaggc cctgcaggag ccggccgtgc gcaccaagct ggctgcgcag gggctgtttg
     6781 cctccggcac cacaccggct gagtttgcgg cgcagatcaa gaaggaaatc gacaagatgc
     6841 agcgcatcgc gaagttcgcc aagatcagca tcgagtaaag cggaaagagc cggcccgcag
     6901 cgcctgcgcg ctgtggggcc ggtcccatgc acccccggcc gcaacgtggc cttggccacc
     6961 atggccaccg caggggtgcc gacaggacca caatcaaaac catgacccca tttctccgct
     7021 ctctgcccgg cagcaccgcc ctggtgctcg attcaccgca cagcgggacc gactaccccg
     7081 acgacttcgc ctacgcctgc gcgctgccgg tcctgcgccg cgccgaagac acccatgtcg
     7141 aaaagctcta cgacttcgcc cacgctctgg gcgcgggctg ggtcgaggcc ttttttccgc
     7201 gcagctacct cgacgccaac cgcgacactt cggagatcga cgtcggcctg tttgacgagc
     7261 cctggcccga tgccgtgtcc accgacacca aggtgatgag caaggtgcgc ctgggcaaag
     7321 ggctgatctg gcgcaccacc gatgacggcg aagccatcta cgcccgtccg ctcagcgtgg
     7381 ccgaagtgca ggcgcgcatc gcgcgctgct gggagcccta ccacgcggcc gtggcccagg
     7441 ccatcgacag cgcgcaccag cgccacggct acagcattca cctcaactgc cactcgatgc
     7501 cggccattgc gtccagccac gcgaccgagt tcccgggcga ggcgcatgcc gacttcgtgg
     7561 tcggcaaccg cgatggttcc acctccagcg agccgctggc ccggctcgtg tgcgaacacc
     7621 tccagggcct gggttacagc gtttccttca accaccccta caagggcgtg gagctcgtgc
     7681 gccgttatgg cgagccgctg cagcagcgcc acagcatcca gctcgagatc aaccgcaagc
     7741 tctacatgga cgagcagacg ctggccatgc acgaaggggt tgcgcctctg aaggccagcc
     7801 tgcagtcgct ggtgcacgtg ctgctggcca ccgatccgcg caccctgtag tgccgcgttg
     7861 gcgggcaggg ctcgcggata atcgtctgcc atgatcaccc tgttcagcga ccaacacgcc
     7921 ctgcatgcgc ccgccttcga atttttccgg ggcgagcgtg tgccttgttt tgaaaccccg
     7981 gcgcgtgccg tctttgtcca gcagcgcctg ctgcagggcg gccacacgtt gcgcgagccg
     8041 catgtcgaca gccgcgcggt gctgggccag gtgcacagcg cgcgttacct cgcgtttctc
     8101 gagcatgcct gggacgactg gctgagcctg gacccgggca acgcggaccg ccagcctttt
     8161 ccgtcggtct ggccggtgcg caccctgcgc agcgacatcg aaccggccaa cttcattgca
     8221 cgcctgggcc tgtattcgat ggacaacgga tcgccgctgt cggctggcac ctggcttgct
     8281 gccaaggccg gggccgacgc cgcggccagc gcggcggcgc tgatcggtga aggcgcgggc
     8341 gcggtgtttt gctgcagccg tccgcccggc caccatgcgg gtgccgactt catggggggc
     8401 tactgctttc tgaacaacgc cgccgtgacg gcgcaggctt tgcgcgatgg cggcgcagac
     8461 cgggtggccg tgctcgatgt ggactaccac cacggcaacg gcacacaaag cattttttac
     8521 gatcgcgccg acgtgttttt tgtcagcctg cacggcgacc cgctgaccga gtacccgttt
     8581 tacctcggcc atgccgacga ggcgggcgag ggcgccggtc tgggcttcaa cctgaatctg
     8641 ccactgcccg cgggcgcgcc ggccagcg
//
LOCUS       DAIDFG010000115         8627 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_115_length_8627_cov_17.923938, whole genome shotgun sequence.
ACCESSION   DAIDFG010000115 DAIDFG010000000
VERSION     DAIDFG010000115.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8627)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8627
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115_length_8627_cov_17.923938"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1714 a         2750 c         2561 g         1602 t
ORIGIN      
        1 ggtgcgggcg ggcagcacat caacaagacc gactcggcgg tgcgtatcac ccacctgccg
       61 accggcatcg tggccgagtg ccaggagggc cgcagccagc acggcaacaa ggcccaagcg
      121 ctgaaggtgc tgaccgcccg catccatgaa aaagaccgtt ccgaacgcgc cgccaaggat
      181 gcggccatgc gcaagggcct gatcggaagt ggcgatcgaa gcgaccggat ccggacctac
      241 aacttcccac aggggcggct gaccgaccac cgcatcaacc tgacgctgta caagctgctg
      301 tccatcatgg aaggcgacat ggacgacgtg gtcaccgccc tgcaggtcgc acgcggcgcc
      361 gaactgctgg ccgacctgga aacctcgtcc aatggctaat ggttgatggc tgaattttta
      421 atgccaaatt ggccttcagc ccttgatcaa tgggcgtcac cagctcctgt tttcatagca
      481 gtgcaccgtg actgaatccc ttcccaaaaa caccctcacc tgcgcgcagg cgctgacagc
      541 cgcccgcgga ctcgggctgg agcggctgga cgcccagctg ctgctgctgc acgcactcgg
      601 caaacccggt actgaccgcg cctggctgct cacccatgac acaacccctt tgccggcaga
      661 ggcaacgcag tgtttccgcg aactcagcct gcgccgcgct ggcggtgagc cgctggccta
      721 cctcgtcggc agcaaggagt ttttcggcct ggaactgcag gtggatggcc gcgtgctggt
      781 gccgcgtccg gacacggaaa ccctggtgga atgggccctg caacaactgg cccagacgga
      841 catgccggcg tccgcccggg tgctcgacct gggcaccggc agcggcgcga ttgccctggc
      901 gctgaagaaa acccggccgg cgctcgacgt cacggcggtg gatgccagca gcgatgcgct
      961 ggcggtggcg gctgccaacg cagggcagca ccagttgccg gtccatttcc tccagggcaa
     1021 ctggttcgaa aatgtaagtg cccgctttca cctgattgtc agcaacccgc cttatgtggc
     1081 cgatgccgat ccgcatttgc ccgccttgcg ccacgagccc ccgcaggcac tgacggccgg
     1141 aaaagacggc ctggacgatc tccggcggat catcgaacag gcgcctgcct acctgcagcc
     1201 gcaaggctgg ctgctgctcg agcacggcta cgaccaggcc cgggcggtgt gcgagctgct
     1261 ccggcagcgg ggctttggcg atgtgcaaag ccgggccgac ctggcgggca tcccccgctg
     1321 ctcgggaggg cgctggcccg accctgctcc gggcaccggc agccaaacgg cgggataatc
     1381 acccattgat ttaccgcgtc cgacatccat ttatttgaac aaggcaaagg ccggctttcc
     1441 ttcggccacc caacacttcc atccaaagga cccctcatga acgataccca gcaacgcatt
     1501 gaccagatcg tcaagtccag tgaggttgtg cttttcatga aaggcacggc ccagttcccc
     1561 atgtgtgggt tttccggacg cgcgatccag gtgctcaaag cctgcggcgt gaccaagccc
     1621 gccaccgtga atgtgctcga ggacgaagag atccgccacg gcatcaagga atacagcaac
     1681 tggccgacca ttccccagct gtatgtcaaa ggcgaatttg tcggcggctc ggacatcatg
     1741 atggagatgt accagagcgg cgagttgcag caggtgctgg gcacacccgc cgaataaaac
     1801 ccttcccgga ccttcccgca ggcctgcccg tcaccggcag gtcgtggagc tctcaggtct
     1861 gacgcccttt ttactgggta tacccttcac acgcccccgc attgacacgg cccttgcact
     1921 cgcgcttgag ccttgcagag cgctccctgg cggtttcctg cgagccgggc atgaacttga
     1981 cgctttttcc cggtggggca ctggcctttt tctgggcctt cttgctggcc gccaggctgg
     2041 gcccagccga gaagccaagc agcagggccg ccgccaacac ggccgccgag cggcgtgcca
     2101 atagtgcttg aatcatgtcc ctctcctcct cgttgaccga cggcctgcca tttgcagtcc
     2161 tgccgttttt acacgatgac aggccctatt gcaatctggc gtcgccgtag acctggtcat
     2221 ccggcggcgc atcggctggc aagcgaaaac cctcgctggc ccaggcgccc agatcgatat
     2281 ttttgcaacg ctcgctgcaa aaaggcctga aacggttttc ctgcgcgtaa atgctggggc
     2341 cgccgcaggt ggggcagacc actgttttgg gtgggggctg gtctggattc atggtcgcaa
     2401 gttcaaactc aaaatcaggg gtcaggatcg gcgtccacgg accggcctca ggaacaaagt
     2461 gtcaattcga aggcggcgtc gtcggtacag gggtgtagcc ggtcgtcact ttcgtggcgc
     2521 atgaggcgca cggaaaccat caggcggttg ccgctgatct ccggaatcaa ccccaggctg
     2581 gggtcgatca gcaggcgcag aagctggaag gtacgtccct gcggcagggt ttgctggtac
     2641 tgcccgcccg acgccacgac tttttgcggc agaccggagt cgcgtagcag cttgagcaac
     2701 atccggaccg actcggccag cggcaccagt gtggcaatcc aacgctgcaa gtcctgttgc
     2761 cgcaggtgtg tttcaaagtg ctgccaggcg aaataagccg gcagatcgaa ttcacaggtt
     2821 cccccgggga tgccgatgcg gctgcgaatg ctcatgagcc agtcgttttc agtcagggac
     2881 tgccccgctt ttccgggcag gctgttcaaa gccgcaaaac acccatccaa ctgtccggtg
     2941 acctcgtcga gcaccgcctc ggaaatcgcc gggttgccgc ggtagccgtt gagcgtgtgt
     3001 ttctgccgat ccaggtcctt gagtacatcc gttttcaggt cggcacgggc cgccacgtcc
     3061 atgatttcga aaatcgtcgt gatggcgtaa tggtggtcag tcggatgttc gcgcgcggcc
     3121 agttcgccca agcggtgaaa cagatgctca agtcgcaaat aggtacgaat gcgttcgttg
     3181 aagggatact cgtaaaggat cactgcgata tatctttttc agcgggttgg tggtccatcc
     3241 atcatagccc gaagcgctgc gacagttgcc gtaccatgac cgccagcgcg gccagcgaca
     3301 ggggcgcgtc gttgtaaatg cagatgtcgg cggcggcgag ccgctggtcg cggctggcct
     3361 gcccggccat gacgcgttct accgcttcac gcgtccatcc gttgcgtgcc atcacccggc
     3421 tgacctgggt tgcctcgctg cagtccacca ccagcacctg gtctacacgc tggcgccagc
     3481 gccctgactc gaccagcagg ggaacatcaa atacgataca ggcgctggcg gcctgagctg
     3541 cctggcgggc ggtttcttcg ccgaccagcg gatgcacaat ggcctccagc tgcttttttg
     3601 ccccggcatc cgcaaaaacc agctcgcgca tcgcgtcgcg gttcaagccg ccttccggtg
     3661 tgatgaaccg ggcaccaaac tgcctggcaa gcccgtccat ggcggcgccc cccggtgccg
     3721 tgacctgacg cgagatggca tcggagtcaa tcaccgcggc gccccagtcg gccagcatcg
     3781 cggcaaccgt gctttttccg ctgccaatgc cacccgtcaa gccaacgcgc aacgcccgca
     3841 tccggctaca gcccgatgaa gcgcaggatg gactgggggc caaacaccat ggccgtcagc
     3901 cccgcacctg ccaggaaagg gccaaagggc acataccctc cttcgcgcaa gccgctggag
     3961 aacttcaacg caatgccgac cagggcacca atcaccgacg ccatcaggat catcggcacc
     4021 agcgcgctcc agccgaacca ggcccccaag gccgcaaaaa gcttgaagtc cccgtaaccc
     4081 atgccttcct tgccggtcac cagcttgaaa gcccagtaca ccagccacaa ggacagataa
     4141 ccggccaccg tgccccacag ggcatcggtg agctgcaccg ccgggttcca gcccagcgcg
     4201 gcgacgatca agccggccca cagcagcggc agggtgacgt cgtcgggcaa cagcgtggta
     4261 tcccagtcaa tcagcgccag tgccagcaag gtggcggcaa aaccgctcca ggccaggccg
     4321 gtggcggtca agccccagcg ccaggcgcaa aaaaagaaca aggcgcccgt gaccgcttcc
     4381 accagcggat agcgccatcc gaacttcgtc ccgcagacag aacattttcc cttcaggaaa
     4441 aggtaactca ggagggggat gttctcgtac catgcaatca tatggccgca gttggagcag
     4501 cgggagcggg gcaccatcaa attgaatttt tcggtgccgt ccacagcctt gccggcaagc
     4561 tcggcacatt cggccgccca ctgccgctcc atcattttcg gcaagcggta aatgaccaca
     4621 ttgaggaagc tgccgaccag cagccccagg atacctgcca gcgcggcgtt gagttcggca
     4681 ggcaaaccga gcgcgagccc gggcatcaga ccacttggcc cagtttgaag atcggcaggt
     4741 acatggacac cacgatcccg ccgatcaggc cacccaggaa cacgatgatg atgggctcca
     4801 tcaggctaga caggcccgcc accatctcat cgacctcggc ctcataaaaa tctgccgctt
     4861 tgccaagcat gtgatccaca gagccagact cctcgccgat ggcgcacatc tgcagcacca
     4921 tggtcggaaa caggttggca ttacccatgg ccgccgtcag gctggtgccg gtcgagactt
     4981 cctgctggat cttccgggtg gcgtcctcgt aaagggaatt gccggaggcg ccgcccaccg
     5041 agtccagcgc ctcgaccaac ggcacgccgg cagcgaacat ggtcgacagc gtacgcgtcc
     5101 agcgggcgat gcaggacttt tcgatcaggg cgccaaacac cggcattttc agcaggagcc
     5161 gatccatgaa tcgctgcact ttttcgttgc gcttccaggc ctgcatgaaa aagtaaatcc
     5221 ccccccctac gctgccaaaa ataagccacc agtacgccac gaaaaactcg ctgatggcca
     5281 tcacaaacag tgtcggggcc ggcagatccg cgccgaaaga ggagaacacc tccttgaacg
     5341 cggggatcac gaaaatcatg atcaccgcaa ccaccacaaa agccaccacc accaccgagg
     5401 tcgggtacat cagtgccgac ttgattttcg acttgatggc ctcggttttt tccatataga
     5461 cggcaaggcg atcgagcaac tgctccagga taccggcggc ctcgccggct tccaccaggt
     5521 tgcaatacag cgcattgaag tacaacgggt attttcgaaa ggcggcgctc aaggacgtgc
     5581 cggtttccac atcagtccgg acgtcattga gcaatttggt cacgctggca ttggcgttac
     5641 cgcgtccgac aatgtcaaaa gcctgcagaa gcggcacacc ggccttcatc atggtggcca
     5701 gctgacgcgt gaagatggcg atatcccggg gcttgatgga cttgcccgaa cgcatactgc
     5761 gcttcttgat cttggtcgcc aggacaccct gacggcgcaa ggaggccttg acctggtttt
     5821 caccgaccgc gcgggtttcg cccctcacct gcttgccatt gcgatccttg ccctcccact
     5881 cgaagacaaa ttccttgatg tctctggatg ctgcagttgc catggtgcct tcttcagtag
     5941 gaaagtgatg atggggactc gaaatgtcca gcgacgcgac aaacccgctt gttgaggggc
     6001 aagggcttaa acattggtgc aggccagaat ttcttcgagt gaggtcatgc cgagtttgac
     6061 cttgtgcaga cctgctccgc gcaggctttt gacaccttcg ctttctgcct gcgcagcgat
     6121 ttcgagtgcg ctgccatcac gcaggatgat ccgctggagt tcctcggtga tgggcatgac
     6181 ctggtaaacc cccacccggc ccttgtaacc gttgttgcac atggagcagc ccaccggccg
     6241 gtaaggcgtc caggtgccat ccacatcatc ctccttgaaa cccgcgtcca gcaaggtctc
     6301 gcgcggaatg tcagcggggg ccttgcaatt ggggcaaagg cgccgcgcca ggcgctgggc
     6361 cgtgatcagg atgacgctcg aggcgatgtt gaagggggca atgcccatgt tgcgcagccg
     6421 cgtcagcgtg gccggcgcat cgttggtatg caaggtggat aacaccaggt ggccggtctg
     6481 ggcggccttg atggagattt cggccgtttc cagatcgcgg atctcgccga ccatgatgat
     6541 gtccgggtcc tgtcgcaaaa acgccttcag ggccacggaa aacgtcaggc cagccttgtc
     6601 gttcatgctg acctggttga cgccaggcag gttgatctcg gccgggtcct ctgccgtggc
     6661 gatgttcacg cccggcttgt tcaggatatt caggcaggtg tacagggaca cggttttgcc
     6721 ggaaccggtg gggccggtca ccagcaccat gccgtaggga cgctgcacgg cgttgagcag
     6781 gcgctccttt tcaacaggct cgtaacccag cgcatcaatg cccagtttgg cgctgctcgg
     6841 atccaggata cggatcacga ttttttcacc gaacatcgtg ggcagggtgc tgacccggaa
     6901 atcgatcacc cggtcgggcc cgattttcag cttcatcttc ccgtcctggg gaatccgctt
     6961 ttccgaaata tccattttcg agatgacctt gatccgcgag gccagctttt ccttgatcgc
     7021 cacaggcggc gaggcaatct cgcgcaactc gccgtcgatg cgaaagcgca cccggtaggt
     7081 gtgctcgtaa ggctcgaagt gcaggtccga ggcccgcatg ctgaacgcgt cgagcagcat
     7141 cttgtgcagg aacttgacaa ccggcgcgtc ctcgacttcc gaaacaccgg tgtcgttgct
     7201 gtccgcgctg gccgcctccg cggaggactc atcaaactca aaatcatcac caatgatgct
     7261 gtccatggct tcggtcgcag tggtggcaga cgcctccacc atcttggaca gcttgtcgta
     7321 ctcggcaatg acccagtcca cgcccatctg ggtggaaaac ttgatttttt ctgcggcttc
     7381 ctggtcggac gggtcggcgg tcgcgacgat caggcggttg ctgcgcttgc tcagaaccac
     7441 aatccggtaa gcctgacaga ttttggtgtc cagcaaaccc ttgggcaagc gctgtgtatc
     7501 tatcgcattc agatcaagaa ggggggccgc aaacgcggtg gacatggtgt gggcgaggtc
     7561 cgacggggac accgcgccgg agccggtcaa ttcggcaata aagctggtac gcccgctctg
     7621 ggccttgcga aagatgtcct ccgcggcctt ctggcccagc ttgccggcaa tgaccaacgc
     7681 cctgcccaag ccgggcaatg ccagcgtggc gggtgcattg cttacagaat cgacagctgc
     7741 catggttttg agaatccagc ccgaattgat aaaattgaca tcatcgccga tagatctggc
     7801 ggtgtaaatc taaaagcgtt ctgcctttgc tccggtgttg gaacaaagca aagtcagaca
     7861 tccgcggacg ggatgcgggg ctcggaagcg gaccatgaat ggtcggggtg agaggattcg
     7921 aacctccggc ctctacgtcc cgaacgtagc gctctaccag gctaagctac accccgaaaa
     7981 atgccgcctc aggcgtcagg aacgacgctg cagcagctga gaagccaatt gtagcaaggc
     8041 ctcgctgtag gggttgaccc ccagtgccgc caaggcctgg acagcgcgtt gagcctccgc
     8101 ggccgctgca tcccgggtcg cctgcagggc accggtcttg cggacgatcg ccactatctc
     8161 ggccagctgc cccgtcccgc cggtctcgat ggcctgccgg atggtggcct gctcgtccgc
     8221 ggtgccgcgc tgcatggcga tgatcagcgg cagggtcgcc ttgccctcgc gcaggtcgtc
     8281 ccctaggttt tttcccattt cctcggcgtc gccgtcatag tccagcacat cgtcaatcac
     8341 ctggaaggcg gttcccagcg cctgtccgta gtcggcgcag gcaagctcta tgtcagccgg
     8401 cgcctgcgcc agcaatgcag ccagccgggc gctcgcctcg aacaacttgg cggttttgga
     8461 gcgaatcacg cgcagatagc cttcttcgga cagaccggca tcgtgcatgt tcatcaattg
     8521 cagcacttcg ccttcggcaa tgatgttggt cgcctcggcc agggtttgca tgatgcgcat
     8581 gtcgccggca tcgaccatca tctggaaggc cctggaatag aggaaat
//
LOCUS       DAIDFG010000116         8602 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_116_length_8602_cov_15.417690, whole genome shotgun sequence.
ACCESSION   DAIDFG010000116 DAIDFG010000000
VERSION     DAIDFG010000116.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 8602)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8602)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8602
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116_length_8602_cov_15.417690"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1593 a         2685 c         2665 g         1659 t
ORIGIN      
        1 ggtggacgtg ccattggtca cgaaaaagca gtggtcggca ttgaagatgc gggcggcatt
       61 gcgctccgag gcgcccaccg ggccgtcgtg gtccagcaac tggcccagct cctcgaccgc
      121 gttgcagacg tcggcgcgca gcatgttttc accaaaaaac tggtggaaca tctggccgac
      181 cgggcttttc aggaaagcca cgcccccgga atgccccggg caatgccagg aatacgaacc
      241 gtcttcggcg tagtcgagca acgccttgaa aaacggcggc tgcacgccct ctaggtaact
      301 cttggcctca cggatgatgt ggcgcgccac gaactcgggc gtgtcctcga acatgtggat
      361 gaacccgtgc agttcgcgca ggatgtcatt ggggatgtgg cgcgaggtct tggtctcgcc
      421 atacacgtag atcggcacat ccaggttctt gcgccgcact tcctcgatga agtggcgcag
      481 gttcagcacc gcggggtcca gatccggccc cggcgtgaac tcttcgtcgt caatcgacaa
      541 aatgaaggcg ctcgcacggc tttgctgctg cgcaaactgc gacaggtcac cgtagctggt
      601 cacgcccagc acctcgaaac cctcggtttc tatggcttgc gccagcgcgc gaatgcccag
      661 cccggaggtg ttttcggagc ggaagtcttc gtcaatgatg acgatgggaa agcggaattt
      721 catcaaaagg cctttgcctg gagctgccaa aaaaaacgga tcagggccga agtgtaagga
      781 tatttggtga aggcagagtc ggtccggcct ttgaagcgcc cggttaagct tgccaagggg
      841 cttttgtggc tcaaaatgtg gcttttagga aactaaggaa acgcgacatg acaagcttgg
      901 ccgattcgac gatttggtgg ctgctggccg gtggcgccgt ggcgctggag ctggtcacgg
      961 ggacgtttta cctgctgatg ctggccgtcg gcatgacggc tgcggccctg gcggcccatc
     1021 tgggggcctc cgtcatggtg cagattgtgg ttgcggccgt ggtcggcggc ggcgccgtgg
     1081 tggcctggca ctggaaacgc agcaaaagcc ccaggcccgc ccaggccaat gccaaccggg
     1141 acgtgcagat ggacatcggc gagaccgtgc atgtgcagca gtggaacgac gatgccaccg
     1201 ccactgtgaa ataccgcggt gcccaatgga cggtggagct cgccgaccat gacgccgcac
     1261 cccagcccgg cctcttcaag gtcagaaaac tcaacggcaa ccgcctggtg gtcggcaaac
     1321 tgtgagaaag tgcacggtct gatgcccggc agctgatgct gcccctgttt tttgtttttt
     1381 acggagaaac tcatggaaat tgcactcgtc atcctggtga ttgccgtcat ttttatcgcg
     1441 cgcagcatca aggtcgtgcc ccaacaaaac tcgtgggtgg tcgagagact gggcaaatac
     1501 cacgccagcc tgacaccggg cctcaatttc ctgatcccct tcattgaccg cgtggcctac
     1561 aagcactcgc tcaaggaaat cccgctcgac gttcccagcc aggtctgcat cacgcgcgac
     1621 aacacccagt tgcaggtcga cggcatcctg tatttccagg tcaccgaccc gatgcgcgcc
     1681 agctacggct catcgaacta catcgtggcc gtcacccagc tggcccagac ctcgctgcgc
     1741 agcgtcatcg gcaagcttga gctcgacaaa accttcgagg aacgcgacat catcaacgcc
     1801 caggtcgtgg ccgccatcga cgaggccgcg ctgaactggg gtgtcaaggt gctgcgttac
     1861 gagatcaagg acctgacacc acccaaggaa atcctgcacg ccatgcagtc gcagatcacc
     1921 gccgaacgtg aaaaacgcgc gctgattgcc gcctccgaag gccgccgcca ggaacagatc
     1981 aacatcgcca ccggtgagcg cgaggccttt atcgcccgct ccgaaggcga aaaactcgcc
     2041 gccatcaaca acgcgcaggg tgaagcggcc gcgatcaccg cagtggcaga cgccaccgcc
     2101 caggccatcg aacgtattgc cgcctcgatc cgccagcccg gcggtgaaat ggccgtgcag
     2161 ctcaaggttg ccgaaaaggc cgtggaagcc tactcccgcg tcgcggccga cgccaagacc
     2221 acgctgattg ttcccagcaa catgaccgaa gtctcggccc tgatcacctc ggccatgacc
     2281 atggtgcagt ccacccgggc cgcctgatca gcatcgcaga aggcacccac atgagcgcca
     2341 tgaacgtact cggtacggag ctggtcccct gctcctacga cccgctgacc ggttatttcc
     2401 gcgacggctg ttgcaacacc gacgagtccg actcgggttc gcacctcgtc tgcgtcaagg
     2461 tgaccagcga cttcctggcg ttttcggtag ccaaaggcaa cgacttggtg accccgcggc
     2521 ccgagtaccg ctttgcgggg ctcaagcccg gcgaccggtg gtgcctgtgc gccaaccgct
     2581 ggcgcgaggc actggaagcc ggctttgcgc cgcccgtggt tctggagtcc acccacctca
     2641 gggcgctgga gttcgtgaca cgcgcgcaac tggaaaaaca ccgctttcgc agcgcgcttc
     2701 actgatcagc gccatcttcc gtgccgcgca ctgctacaat gtgaggcttc gcggaaaggt
     2761 ggatgagcgg tttaagtcac acgcctggaa agcgtgcgtg gggttatacc caccgcgggt
     2821 tcgaatcccg ccctttccgc caaaagtata gttgccgtga gttttccaca cggcagtaaa
     2881 agctcctgaa agcccagctt gtctgggctt ttttgtgcct gtcattttcc tgatttccgc
     2941 atagagctcc gcacaccggc ccccgtctgg gcaccgcgca cggtgacggc caccgcagac
     3001 aacttcctgt caccgcgttg aagcaaaaaa aaagccacct tgcggtggct tttttgctgg
     3061 gggcaggaaa acttattcca gggccgtttt cttgccgtcc ttgtaggtgt acaaggtaat
     3121 ggacgggttc ttcagttcgc cgttaggcag gaactcgatg gtggtggtca cgcccttgaa
     3181 gctggttttg agcagctcgg ggatgtagac cttcggctcg ctcgagttgg cgcgtttcat
     3241 cgcgtcgacc agcacgaagg tcgcgtcata ggtgtacggg ctgtagacct ggaactggtt
     3301 cgggtacttc tcgtcgtagc gcttcttcca ctcgacgccg ccaggcatct tggccaggga
     3361 tgcgccgcct tcggcacaaa tcacgttgct cagggtcttg gcgccagcag cgagtttggc
     3421 aatctcggag gtgcagatgc cgtcgccgcc gaagtacttg accttgctca tgcccagctg
     3481 ttccatctgg cgcagcatcg ggccggcttg cgggtccatg ccaccgtaga acacggcgtc
     3541 agggctcttg gccttgatgg cggtcaggat ggccatgaag tcggtggcct tgtcggtggt
     3601 gaactgctcg tcaaccacgg tcatgccctt ttccttggca gtcttcttga agatgtcggc
     3661 aacaccctgg ccgtaagcgg aacggtcgtc gatgacggcc actttcttca gcttgagcgt
     3721 atcggcggca taaaacgcca ggccggcacc cagggccaga tcgttggcaa tgatgcggta
     3781 ggtggtgttg tagcctggct tggtcaggtc agggttggtg gcggcaccag tgacgtgagg
     3841 gatgccgcag tcgttgtaga ccttggaggc aggaatggtc gtgcccgagt tgaggtgacc
     3901 gaccacaccg gcgactttgg agtcgcacag tttttgtgcg gcagccgtgc cctgtttcgg
     3961 atcagcagca tcatcttcag cgacgatttc gaaggtggcc ttcttgccgc cgatcatgac
     4021 gccctgtttg ttcaggtcct caatggccat gcggacgcca ttttcattgt ctttgccgta
     4081 atgggcctgc gcgccggaag tcggggcgac gtgaccgatc ttgacaacga gcgtatcacc
     4141 ggcgggtgcg ggagccgcag cgaccggggc agcaggtgca ggtgcagtgg cggccggcgc
     4201 agcttcttct tttttgccgc aggcaacaac caacatggcg gccaaagcga ccgcagtcca
     4261 tttcatttgc ataaatacac tccaattagg aaattaccgt taatggttga gaactgattt
     4321 ttttactcaa cagccacgaa cataacgcaa tttccgcgcc cgcaccatag caagcagccc
     4381 tagtgttgcg ctatggaggt ggagtaagaa acgaaattga cctgctatta gcgaatctaa
     4441 taaagggcga gaagcagccc cgtcacaagc ggtaacgcgg gctgcccgac atggccagtc
     4501 atcggcaaga accgcccgcc tgaggcaaag acgtcaccca aatgcagccg tctcaggtat
     4561 ttttcaccac ctcgaagaag ccgcccgcat gaacatggaa agccaagggc gcagccagtt
     4621 cagggcttgt ccggcagctg gctggccagt tcctgttcaa aggcctggtt gaccgactgc
     4681 cgcaccacga tctcgatttc gtccctgagg ctgggcgcca gttcctgggt gtgctccagc
     4741 accagacggc ccacggtttc gcgcagcagc cggtccagcg tcaaatcgac gcgctgcatc
     4801 acgcgatgca ccatctgctc ctgaaagtgc tgcatgtcca ttgccgcggg agcgggccgg
     4861 cccccgggcg cggcggcctg gggctgcacc acttccgtca gcgtgggcac aaatcggggt
     4921 ggcgggcggt tcatgttgcg cccgcgctgg ccagatcgtg ccgcttcatg gcataaccgc
     4981 ggtcggcgta atgcttccag cggctgcggg cggccgcgcg gtcgtcgtcg gccagggtga
     5041 cgagttcgat gaagcgctcg aaacgctcaa acccggccgg cacccggtgc ccgaggttga
     5101 ccagcacctc atggtgggga caggcctcca gcgatccggc cagcaccacc ggcgtggcag
     5161 acacggtgga cggggtggcg ctcaggtggc aatgcggcac aaactccagc ggggaaaagg
     5221 tccagagcaa ctggtcgagc cgggccagca tgtccggctc ggccgtcacc accacacgcg
     5281 cgccgctgcc ccaggccttg cgcagcagcc ggcagctgta ggccagcttg tccggcacgt
     5341 tgaaatgaaa agcaacttcc gtcatgcgcg tgaccgggct cccaccttgg gggaacgggt
     5401 tttggctggc gccctggccg gctgaacctg gctcagcaaa aagtcgacca gcaggggcac
     5461 gggccggccg gttgcgcctt tggcggcacc gcttttccag gccgtgccgg caatgtccag
     5521 gtgcgcccag gggaacttgc cggcaaaacg ctgcagaaac ttggccgcgg tgatggcgcc
     5581 gccagcacgt ccggcgacgt tggccacgtc cgcgaaatgg gttttcagcc cgtcggcgta
     5641 ttcgtcgtcg agcggcatgc gccagcacag atcgagcgag ctgtcgccgg ccgcggccag
     5701 ggcctgggcc agcgcgtcgt cactggagaa catgccgctg cgcacgccgc ccagcgcaat
     5761 cacacaggcg ccggtcaggg tggcaatgtc caccacggcg cgcggcttca ggcgttcggc
     5821 atacgtcagc gcatcacaca acaccagccg cccttcggcg tcggtgttga ggatttcgat
     5881 ggtctgcccg ctcatgctgg tcaccacatc gccgggcttg atggcgcggc catcgggcat
     5941 gttttcgcag gccggaatca ggcccaccac attgagcgcg ggctgcagtt cggccagcgc
     6001 gcggaaggta ccgagcacac tggccgcgcc acccatgtcg tatttcatct cgtccatctc
     6061 ggcggcaggc ttgatggaaa tgccgcctgt gtcaaaagtg atgcccttgc cgatcagcac
     6121 caccggcgct tcggccttgg cgccgccgtc ataacgcagc acgatgaacc gcaggggttc
     6181 ttccgagccc tgggcaacgg ccatgaacga gcccatgccg agtttggcga cctctttggg
     6241 gcccagcacc tccaccttga ttccgggcag tttcccgagc tccctggcgg cctgggcaag
     6301 catcgtcggc gtcgcgtggt tggccggccg gttggcccac tccttggcaa gtgcgaggcc
     6361 cgtggccagc ccgacgccct tgtcgaagcc ggcacggctg gccgccagcg aggagacggc
     6421 caccagcacc tgcttgagtt tttgctcggc cggctttgat ttggtggtgc tgtaaaggta
     6481 ggcggcatcg gcgcaggcca gcacggcggc tcgcacggct tcctgaccgg cctgcggcag
     6541 tgcgcagagg ctgagcagca cccgccgcgc attggtgttt ttcagcgcga ccatcgccgc
     6601 gtgcaccgcc gtccggatac ttttggcgct gccgtcgccg gcgcctgcca gcagcacccg
     6661 cgtggccgcg attcccgcgg gccggtaggc gctgagcagc ttgcctgcct tgttttcaaa
     6721 gtcccctgct ttcagggcca acgtcaccat tttcgacacc gcgtcggtgc cggcgctcat
     6781 ctcgccggcc accagcacga tcagggcatc acatttttcg gcacaaattc gggcgggggt
     6841 cagggttttg agttcaaagt ccataatcga gggttaggcg tcacaagtgc aaagtgcagt
     6901 tgtcggttgt tgttaagggc aagtttcagt caatgttatt ccaatcctcg atccgcaaag
     6961 agctggcacg cagttttggg gccacccttg tggttttgat gaccattgtg atcaccatca
     7021 tcctgatccg cacgctcggg caggcctcgc gcggcagcgt caacccgcag gacgtgatgc
     7081 tggtcatggg ttactcggcc ctgggccgca tgccgatcat cctgacgctg tccctgtttg
     7141 tggccatggt cagcaccctc tcacgcatgt accgcgacag cgagatggtc gtctggtttg
     7201 tcagcggccg cggcctgggc ttttttctcg tgccgctgtt ccgcttcgcc tggccggtgc
     7261 tgctggtgat cacgctgatg tcggtgtttg tctggccctg gtccaaccag cagacccagg
     7321 aggtgcggga ccggtacgag cagcgcggcg acctggaacg ggtctccccg ggccagttcc
     7381 aggagtcgtc cagcggcaac cgggtctttt tcatcgaccg cgagaccacc caggaaaaag
     7441 tcagcaacaa cgttttcatc gcctcgaccg aaaaaggcaa gaattcggtg accaccgcca
     7501 gttccggccg cattgagacg gtaggcaagg accagatgct gctgctcgac aacggccagc
     7561 ggcttgaaag cgagatcggc aagtcggccc tgaagatcac cgagtttgcg gaatacggca
     7621 gcaaggccgg cgacagcccg ctgctcaacg gggccaacat ccccgaaaaa tccaagtcca
     7681 cccgcgaact gctgaaatcg cccacgcgga tcaaccaggg cgaactggcc tggcgcctgg
     7741 gcatggcgct ggccgcgatc aattttgtcg tgattgcgct ggcgctggcc agcgtcaacc
     7801 cgcgcgccgg gcgcagtggc aacctgattt ttgtgctgtt cacctttgtc gtttatcaca
     7861 acctgctgaa cctgggtcag agctggatca gcgtgggcgc ggccaatttc gccggctttg
     7921 tgctggccct gcacggcggc gccctgttgc tggcgctggg ctggttgtcc aagcgtcacc
     7981 tgaactggac cttcaagggc gccatgagcc ggcagcgcaa gcggcaggcc agccggcagg
     8041 cgggcaaggt ggccgcatga agaccatacg ccgcctgatt tacggcgagg tgctggcctc
     8101 cattgctttt gtggccgtcg gatttctggc gctgtttttc tttttcgacc tggtcgatga
     8161 gctgcagtac ctgggaaaaa atgcggcagg cgtcgtcagt tccgacgcca acacttacca
     8221 gatccgccat gcgctgctgt atgtggcgct gctgattccc agccacctgt acgagctggt
     8281 gcccatctcg gtgctgatcg gcaccatttt cgtgatggcc cggctggcgc agagttcgga
     8341 atacaccatc ttgcgcacca gcggcctgga accctggcgg gccttgcgcc tgctgctgac
     8401 cctgggggcg attttcgtcg tgctctgttt cgccatcggc gactatgtgg ccccagccgc
     8461 cgaccgtgcc gcccagttgc tcaaggcgcg ttaccagggc cgcatcacca tcggccagac
     8521 cggggcgtgg ctcaaggaaa aacaggccta caacagctac gtggtcaacg tcggcgccct
     8581 gtccgccgac aacgaaatgc ag
//
LOCUS       DAIDFG010000117         8582 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_117_length_8582_cov_17.599859, whole genome shotgun sequence.
ACCESSION   DAIDFG010000117 DAIDFG010000000
VERSION     DAIDFG010000117.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 8582)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8582)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8582
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117_length_8582_cov_17.599859"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1576 a         2671 c         2473 g         1862 t
ORIGIN      
        1 aattgcgcgt caaacggacg gcgatgcacc gcacacatgg tctgcagcgc ccacacaaaa
       61 tcagtttgtg catactcttt ttgcatgact cccttacccg caagctggtt acgggccctc
      121 cctctattcg tatatggcag agcacacagt tgaaacatct cgaaacaccg tcgatgatca
      181 acgaatagga aagcgatgca acaccaaccc ctgtcaatgt tgacagggta ggagttaaaa
      241 caagggtaaa tacttgaagg atctgactgc ggcgccagat tgatggcatg ccaacaaaat
      301 tcagggtgca gcaagacctg tgcgagatgt caagacccat gctgccccac agacggcaac
      361 aaaaaagccc cgtcaacact tggtttacgg ggcttttggg ggtctgtgtt ggactacatc
      421 aaaccggttc ttgggagggt gtcgttccca tcatgggcgc taacccgcat ggatgttagg
      481 ttctgctatg tttgaacact gacgtacccc caaaaatacc cccccgcgac ggcattttgc
      541 gttattcgct gatcaccccg aaaatcgagc gcgcccttct gcttgcggcg taagccaatc
      601 attgccagcc ctataaggaa caccatgagc accataaatc aggccctgaa agatcaccta
      661 gtacgccaat tgaaatttct gacaacctca tgcgtcacgt ttgacgcagg tgatagagaa
      721 gaggcgctac ggatcgcgac cgctatacgt gtcattttct atgacaccac aaacagtcat
      781 tcaatccttt atcagatggg cgtcaaggca tcaattcagt tgatctcgac cactaatcca
      841 acttcaaccg ccaatgccct cgctatgttg ccgaccatcg atttcgggtc attctttccc
      901 atcacacttg ggaaacatct ggactacacg ccaccgagcg tcggcgcttt aacgcagtca
      961 gtcgaagact ggtggaacca gcccgctttg tacaccaacc aagttttatt gacgcgggga
     1021 cgtgtggtgc ttgcagccgc ccacaaagat ggcggagctc acgtcgatct aaaaaaggat
     1081 ttggacgaca tcaacgcctt gaaagatggg caaatgttta accgcatcat taagcaagcc
     1141 gacggcaccc agaaagagga acggatcgcc gatcatcatt ttgctttatt gcgacatttc
     1201 gccgctgaga tcttagcgag tcccgatatt caggcgttac agacctaggg gaatgcgggc
     1261 caattccaca gtccttttcg agggagtttc tttgcgtggc gaccagttcg atttctgccc
     1321 acgcccctcc agacttttta ctccctatat ctatgtgccc cttatagtgc acccgtcacc
     1381 gctaggaatc gcgccgtaaa ttcgcagaac gcatcttcgc gcctttgctc acaggcttcc
     1441 agttgatcta tcagccggtc ataggtgtaa tgcctcatgc ccttgggtcg ccgccaatac
     1501 tcacccagcc gggcctccag ttttgattgc ttgcgccaca tccgatccag cacatcacct
     1561 gactggctgg catagctcac ccgctggcag tgcctgcagg caaaccgccc cgcgcgcatg
     1621 tacagcacac ccgcgcgtcg ctggcagacg gggcaaacaa accagggccg ggacttgccg
     1681 taattgcagg ctgtatgcgc aagccggata gtttggctcc catcgcgccg ctggtcgtca
     1741 ctgcccacca tgtattgcag ggccagcgag tcagtgccat gcaccaaaac cccaatgctg
     1801 cccgtaggtt catcgccccg atgccagctc caggtaaatg actgccctgc gcgcaaatag
     1861 ccgccgcgat gccatcggcc aatttctacg cgtagcaggt gttccgcctt gagcttgtag
     1921 ctgggcctgc cagcgccgta cctcatgccg cctctactca ttcgccacct ccacagaaaa
     1981 cgaaatcaat aggccaactc gccttttttc aatcaaggca acggccatca accgcccccg
     2041 gtcacgcgcg cgcacctttt cgcctttttg cccatgcctg tatctgtggc tttgaatgcc
     2101 tgcaatagat ggtatttcta gatactcccc gcctatcggg ttagcgcatt gactgaaagt
     2161 ggccggatag ccccgatgag cggggcaaag cccgttacac catcgccaga aatcggggct
     2221 gttcataccc ttcctacccc gaccttcggg ttaagcgccg caagtctgta ctgccctgtc
     2281 cgataggttt ttggatcaag gtctatgccg tccgtcacgt ccagggcata ccagcccaat
     2341 gcaaaccagg ccgcacggtt aggccgcata cccatgcggg tttcaatcag cagccccgcc
     2401 tccttgactt ccgtcagcgc gcgcgaaacg gtgtcgtgac ttcgccagcc catcggcttg
     2461 aggtacttcg cgcaagccac cagccgcccg ttattgctgc ccgtgtactg gcgggcaata
     2521 tcaagcagca aggcgcgcgc agcgtacccc aaggcgcgat agctggggct gtcaatcacc
     2581 acatgcggta aggccacgta gcgatcaccg tcccgcttgt ccttactgcg cttgtagctc
     2641 ttgctcacgg cctgcctttg tgcgtttagc caggtactcg cgcgccgcgt ccaggctatc
     2701 caggggcttc caaagcccat agcgggtggc gtagtaaggc gtctggccga tcacctccgg
     2761 gtcgccttta atcagctcta tgccgatcaa ggcgaattgc gccgccagcg ttgcataggc
     2821 tttgcgctca cgctgcccgg taggcaggtc aaggtcgttg tgtaccgcgt caggcgtgtt
     2881 cattgcttgc cctcccgcgt atacaggccc acacaatgcc tcaccccggc ctccgtcacc
     2941 acggtctgcc tgtgcgtcag gattcggtgg ccttccttgc gcagctgcaa tatgcgcgcg
     3001 gggcagtggt aaatgtccag gtagcgcatg gcctcgaacg tggtgatggg aaaccgcgcc
     3061 agggcctcca gcagccgcgc gcattgcgta tcggcactgg tgcccttaaa ctctgccctg
     3121 atagcttcta gggccgcttt cttttcgggg gaggtggcct tatttttttc catggcttgc
     3181 ccgcttactt cgctgcctgc gctttgagaa aagcgtctat ggcgttgatg tcgaacagcg
     3241 tgactttttc cccggccttc aggggctgcg ggaaaccatg gcgggatttc gtccactgcc
     3301 acagggttga ccgggctatc tggaaatgcg cgcaggctgc tttggcgcgt gcgtagtggg
     3361 gcttttggtc ggtgccttga accgaggttt tgctgaccat caaataactc caagtgtgtc
     3421 cagcgccgtg gtggctggca tggagtcgat gatgttggtc taggctgtgg aagtcattac
     3481 ccgcctgcca gctacccggc cggtttgcct gcaggcgggg tatcgcgcag ccagacctcg
     3541 cgcagttgtt tctccgttac cgtcacattc atttcgttcc acacacgtcg cacatagtca
     3601 cgtgcgaatg cggatttgtt gtccccgtag ttctcgcgat gcaaactcca ctccgcttgg
     3661 acgaattctt tcgctttcct gtgtggagca tgttttaaca gtgctttggc ggaatttgtg
     3721 tcactgatga ctatttcaat tacttcgatc atggacaggg ggttagcttt ttgtctaaaa
     3781 acttccatcg cccgcatgtg catatacgca aaggcgtagc gttcactatc accttcaatc
     3841 tcatccaacc tgctcatgtc gggcaacact gcgagcaatg agcgcgcgtc ctctaacgcg
     3901 tcgtctgcca tttggcttgc ttccaaaatt gagcgttcag tcattgcctc aaactcagca
     3961 gccgctaacc ctcgcctccg ataggcaaga actggcctcg attttccctg tagctttcta
     4021 atatgcgaca tgcggccccc gcgcccctga taaggtgcca gtccagcccg tcagggaaac
     4081 gggttttcgg ggatcagcct aggactggca aaaatctttt agttcaagct gccttgcgtt
     4141 tgaaggtcaa cactttgcct gatgtgcagt tctccagata gtcgccccaa tgctgcatca
     4201 tcttggcgcg ttccttgaga taggttgcat ggttgtaggc cgcgctcact tggtcgcgct
     4261 cttgatgcgc caattgcaac tcgatatggg catggttaaa gcccatttca tgcagaacgg
     4321 ttgacgcgat gcccctatac ccgtgcccag tcatcctgcc cttgtagccc atgcgctcca
     4381 gcgccttcaa gatggtgttg ttgctcatgg gcttttcatg gtcgcgctca ccggggaaca
     4441 gcaaagcccc atgccctgat accaattgca gcgtgcgcaa caccgttacc gcctgtgatg
     4501 acaaaggcac gatgtggggg gttttcatct tcatgcgctc aggcggaata tcccagcgcc
     4561 cggcctccag gtcaaactca gcccagcgcg cgcctatcag ctcagaggtg cgtacaaagg
     4621 tcagcgccat caatttcatg gctaggcgcg tgacgggtgc gccttgatag gcttccatgt
     4681 ggcgtagcag gtcgggcagc tctttagcgt ccagccgggc atagttaccc gccttgcgtg
     4741 atgccaatat gtctgcaggc ttgatgtcgg tcgccgggtt gcgctgtgcc agcccatgcg
     4801 cgatggcata gcggaacacc tgccccgatg tctgcaaagc gcgtttggca atatccagag
     4861 cgccgcgcgc cgcgatggcc ttgaccattg ccaccagttc gggggcctga atgtccgaaa
     4921 tgggcctttt gccgatggcc gggaaaacat cggccttcaa acgcttatgc acgtcatcag
     4981 catggcgagg gcttttggcg ctgcgccatt gatcccacca cagcagggcc acggtctgaa
     5041 atgagttttc ggctgcggat tgcttggcca gcttgtcagc cttgcgctgc gccatcgggt
     5101 cgctgcccgt cgccagcagc ttgcgcgccg cgcccatcgc ctcccgcgcc tgcgccaagg
     5161 acacatccgg ataggagccc agggccatca ccttttcctt accgtccgcc cggtacttcc
     5221 agcgccacag ctttgagccg ctttcagtca ccagcaggta catgcccttg ccgtcgctca
     5281 gtttagccgg gctggggccg ggtttggcct tcttgatggc ggtgtctgtc aggggcatgg
     5341 aaggctccaa acggcctttt tggaaaaata cccccaaggt tttgagccga tacccccgaa
     5401 cataccccca aatcatcccg acttgcaata ccccacattg gacgaccgga aacaaaaaag
     5461 ccccgtaaac actgggtttg cggggctttt gggggtctgt gttggactac atcaaaccgg
     5521 ttcttggcgg agagggtggg attcgaaccc acggtacctt gcggtacgcc tgatttcgag
     5581 tcaggtacat tcggccactc tgccacctct cctaaatcga gccgcaattc tatcaggccg
     5641 gcgaacctgt cagcgggcag gcggtgccag caccgtgatg ccgcccatgt agggctgcaa
     5701 ggcagccggc accgcgacgc tgccgtcttc gcgctgataa ttttccagca ccgcgaccag
     5761 cgtgcggccc accgccaggc cggagccgtt gagggtgtgc acaaattcgt ttttgccctg
     5821 cgcgtttttg aagcgggcct gcaggcggcg ggcctggaag gcttcgcagt tggagaccga
     5881 gctgatttcg cggtaggcgt tctggcccgg cagccagact tccaggtcgt aggtcttgct
     5941 ggcgccgaag cccatgtcgc cggtgcacag cagcatcacg cggtacggca ggcccagctt
     6001 ttgcaggatg gcctcggcgt ggccggtcat ggcttcgagt gcgtcgtagc tgtggtcggg
     6061 gtggacgatc tgcaccatct cgaccttgtc gaactggtgc tggcggatca tgccgcgggt
     6121 gtcgcggccg tagctgccgg cctctgaccg gaagcagggt gtgtgcgcgg tgaacttcag
     6181 cggcaactgc gcttcgggca gcacctcgtc acgcacaaag ttggtcagcg gcacttcgga
     6241 agtgggtatc aggtacagcg ccgtgctttc ggcttctgcc gccccttcct gcccgccctt
     6301 tttggcggca aacaggtcct cttcgaactt gggcaattgg ccggtgccgc gcagggaatc
     6361 gccattgacg atgtagggca cataacactc ggtgtagccg tgttcggcgg tctggacgtc
     6421 gagcatgaac tgcgacagcg cccggtgcat gcgtgcgagc tggccctgca tgaccgtgaa
     6481 acgcgagccg gtgagtttgg tgcccaattc aaagtccagc cccaggggct gccccaggtc
     6541 cacatggtcc ttgacctcga aggcatacgc aggtggctca ccgcccaggc cttcaaccgg
     6601 cgtccatttg cgcacctcga cgttgccggc ctcgcccgca ccgtgcggca cgctttcatg
     6661 cggcaggttg ggcacggcca gcagcagggc ctgcatctcg ccctggatct gttcgagccg
     6721 gacgctggag gcctccagct cggtcttgga gcttgtcacc tgcgccatca cggcgctggc
     6781 gtcttcgcct ttggccttga gctggccgat ctgcttggac agggtgttgc gctggttctg
     6841 caactcttct gtcctgatct gcagcgcctt gcgctcggcc tccagcgcct ggaaggcttc
     6901 cacgtcgagg aaagcttgcg ggcttttgcg ggtttccagc cgggcgatgg ccgacggcag
     6961 gtctttacgg agcaggttga tgtctagcat ggggtcattg tattttgtgc cgcggcaggc
     7021 cggccaccgg ggcagggttc agttcaggct ggccgggtcg aggttggcgc cgcagatgat
     7081 caggcagact ttttcgtccg gccgcggcag ataggcgccg ctgtgcagcg ccgccagcgg
     7141 caaggccgcg gcaggctcca ccgccagcct gagctccttc cacagccaca gctgggcggc
     7201 ccggatggcg tcgtcgctga gcagcagcgc atcacgcaca tgcgcctggg tggccgccca
     7261 gcccaagtcc ccaatgcgct tggcgcccag tgaatcggcg gcaatgccac cgacactcac
     7321 atcgaccggc tgcccggctt cgcgcgcccg gaacagcgtg ggcgcgagtt cgggctccag
     7381 cgcgacaacg cgtgcgcgct gctcaaacca gctggcgatg ccggcgatca ggccgccgcc
     7441 gcccacactg accagcacgg catcaggcag gccggcctgc tgctcgatct ccagcgccag
     7501 cgtgccggcg ccgaccacca cctcgggctg gtcgtaggca tgggtcagca acgcaccggt
     7561 ttgctgctgg cgcgccaggc aggcctgcag cgcttccgaa tacgccgcgc cggtgacgac
     7621 gacctcggca cccagtgcag cgagtttggc gcgtttggcc gggctggaca cttcgggcac
     7681 aaacacctcg cagcgcacgc cgagttcacg cgcggcggct gcggtggcaa tgccggcgtt
     7741 gccgcccgac gccacaatca cgccgctggg cgggatggtg ttggacagca ggcggttgag
     7801 catgccgcgc gccttgaagc tgccgctggt ttgcagttgc tcgagcttga gccagacttc
     7861 ggcgcagtcc acaccgaggg ccttgccggg cagcttccac accggggttt gacgaataaa
     7921 gtggctgtag ccctcgtgga acctgcgctg gcagctctcg atttgatagc ggtctggcat
     7981 atctggcata ccgggcctag gactctgcat gcaggtcgct gccgatggcg tccagcgcgg
     8041 ggccgtccag cttgagcccc ttgggcaacg gaaacttgag ggtctcctcg atgccggcca
     8101 tcttgcgtac cgacactgcg cccagcgcct tgatgcggtc tatcacctgc ttgaccagga
     8161 tttcgggcgc cgacgccccg gcggtcaggc cgacgcggct tttaccttca aaccattcgg
     8221 gcttgagctc ggtggcgtcg tccaccatgt aggcctcggt gcccagcttg gcggccagct
     8281 cgcgcaggcg gttgctgttg gagctggtcg ggctgccgac aacgatcagg atgtcgacct
     8341 gcgggctcat gatcttgacg gcgtcctgcc ggttctgcgt ggcgtaacag atgtcctgct
     8401 gcttgggcgc gcgcacgttc gggaaacgcg ctttcacggc ggcgctgatg tcggccgcgt
     8461 cgtccacact gagcgtggtc tgcgtgacca ccgcgagttt ttcggtctgc gccggctgga
     8521 tgcgcgccac gtcggccacg tcttccacca ggtggatgcc gctgtcgagc tggcccatgg
     8581 tg
//
LOCUS       DAIDFG010000118         8582 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_118_length_8582_cov_13.755834, whole genome shotgun sequence.
ACCESSION   DAIDFG010000118 DAIDFG010000000
VERSION     DAIDFG010000118.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 8582)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8582)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8582
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118_length_8582_cov_13.755834"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1745 a         2566 c         2680 g         1591 t
ORIGIN      
        1 gtggtcaccg atccggtgac ctacgccaac atcgtgctgc ccgccgaaaa agccgctgaa
       61 atgacctagt ggagctaagc ggatacccct tatttatgta cagtatcata agcaccatgg
      121 acgagctctc tacttcccat tgggttacca ggtgtgccga gcgattgcac gaccgttggc
      181 acacggtgga gcctgcgcaa ctggaagaag tggcgatgga gctttggcgc gattcacacc
      241 tccgtgcatt gccacctgca gaggcagcag cgctgtggct cagtccggtg accacccagc
      301 aggagtaacg cgggcgggtc gcccgattcc gttagcgaca agtagtggca gcgagcggat
      361 atttttcgcc gaggcaagat ctttctcctc caattagagg gggccgcggc ttgccatatt
      421 cgccacaatt ggaaaccact tcctccgttc ggcgaccaat tcgccagcga atatcctgca
      481 aacctgtcgg aagaaaatag ccactcagca agttctacaa cggggatgac gatgactgga
      541 aatacccaac ggctgaccat actcgcgcaa gatcctgcgg ttaagcaaaa cggtgcgctg
      601 gtcctggccc aggtcgaaat tccctatgaa atgctggccg acggccctgt gggttatcgc
      661 gtcaaggtgg tggatttcga cgcaactacc aatacgcttt accagccgca ccgctatgtc
      721 taccaaccgg acggccagat tgttgatcca tttgcgctac caagtgccaa gctctcccga
      781 gaagcccgtc tcgcgtttga tcagaaactg ctgcaaaacc cgaattttca tgcgcagcat
      841 gtctatgcgg tggtcatgcg aacgcttgcg agatttgaat atgccctcgg ccggcgcgtg
      901 ccctggggtt ttaacggaca tcagatacac atcgcgcccc atgctttttg cgacgcgaat
      961 gccttctatt cggaaaaaga tcgcgggctg atgtttggct acttcgatgg agtgcgtagc
     1021 aagcagcggg tgttcacctg cctgtcgcat gacatcgtgg cccacgaagc gacccacgca
     1081 ttgctggatg gtctaagaac ccgctacacc gacccttccg gtccggacca ggccgccttc
     1141 catgaaggtt tctccgatat cgtggcgctg ctttcggtct tttccctgcg acccatcgtg
     1201 gagctcggac tcacgctcgg cgagggacgg atcatcaagc gagacggact tcggctgatt
     1261 caggaaggca ggctgaccca gcaagccatc gccgactcga ttctttttgg tcttggcgag
     1321 gagtttggcc agcagctcgg tggcgcgcgg gtcaatgcgc tcaggcgttc cgtcgaactg
     1381 gagcccggga aagactatct cgaaaggccc gaatatgaag aaccgcatgc gcgcggcgaa
     1441 ctgttggccg cggcaatgct tcggtcattc ctggccctgt gggttgcccg aatccaacag
     1501 ctcggcactt ttgggcgcca gctttacaac ctcgatatgg tgatcgacga aggtacaaag
     1561 gtggccaacc agctcctgac catggccatc cgggccctcg actattgccc gccggtcgat
     1621 ctggagtttg gcgactacct ggcggccttg ctgacggttg accgggaagt tgcgccggac
     1681 gacgcgcgat atagctaccg tgatgcgatc aaggaaacct tcgccagtta cggcatcacc
     1741 gtaccaggac cagaaaccga tgccgatgcc tgctggaaga cgtttgattt gaacgaagac
     1801 attatttaca agcggaccaa cttcgaatcc atgctcaggg acgaggaaga agtgttccgc
     1861 tttatctggg aaaaccgcca cctcttgaga attgatgatc gcggctatac ccaggtgatc
     1921 tcggtcaggc cgagcatgcg gcagggaccg gacggcttca tgctgcgcga aaccatttgc
     1981 gagtacgtcc agatcgccga tctgtttggc gccgaagtga cggcagttct gaagatcaac
     2041 cgccccgagg ggatgtcgtc ccggcagcgg ctgcgtgcct atggtggcgg tgtgttggtg
     2101 tttgaccagt acgggcgcat caagtaccac attgaacgcc ggttggacga tgcggcaagg
     2161 caaaagcgcc ggctggacta cttgtgggat tcggggggac tggagcaggt gcaagacagt
     2221 cgcaatcaat tcgcgatggc gcaccgcatg cgcgctggcg gacactgaca caccgggaga
     2281 cattatggcg acgagttcaa aaccgattga agcggtcatt tttgcctacc aggtcggctt
     2341 tggcgactgc tttttgttgc gctttgttta tcctggtgaa aagtacaagc atctcctgat
     2401 tgacttcggc accaccgggc tgccagcgga tgtcgcttcc agccacatgg atgaaatcgc
     2461 cagggacatc gtcgacaggt gcggtgggaa gcttgatgct gtcattgcaa cgcaccggca
     2521 cgccgaccac atcagcggct ttgcaaccaa taccaaagga acggcctcgg gcgacgtcat
     2581 cgcaggtctg aaacccaagg ttgtgatcca gccttggacc gaacaacctg acctggccga
     2641 agacgcgacg gcgccggccg ctgccaaaaa acacagcctg gcacttcgcc gaatgggatt
     2701 cactgccgaa aaggtggtcc agttgctcga tgcatttccc aagtccgtct ctccggcact
     2761 caaggaaaag cttcgctttg tcggggaaga caacatttca aataagtccg ccgtccgcaa
     2821 tcttgcaacg atggcaccca aaaccgtcta cacctaccat ggcggccctt ccgggctgac
     2881 ccagtcgatt cttcccggtg tcaccaccca tgtgctggga ccgccaaccc tcagacaaac
     2941 cgatacgatc cgaaagcaac gatcaaggga tccggatgag ttctggcatt tgcagatgtc
     3001 cagcctggat gccgacagcc agctgcaggg caagcccggg gcgctgtttc cgaatcatcc
     3061 ctcggtcgac ggtggcaagc tgcccatgag cgcacgctgg tatgcgctaa ggctaaagga
     3121 cgccaggggt gaacaattgc agcaaatcgt caccctcctg gatgaccaga tgaacaacac
     3181 gagtctgata ctgctgtttg aagccggcaa gaagaaactc ttgtttcccg gtgatgccca
     3241 gctggaaaac tggcaatacg ctttgtcgca aagcagcagt aggacgctcc tgaaggatgt
     3301 tgatctctac aaggtaggtc accatgggag tttgaacgca acgcccaaaa gcttgtggac
     3361 cgacttcagc aagcgcggca aggctggcca aaagaatagg ctgaagacgg tcatgtccac
     3421 cagaccggac aaacatggtc acgaaaaatc caacactgaa gtaccgcgac ggactttgct
     3481 ccaggctttg gacagtgaga gcgaactgca ctcaacgcat cgacttggtg ctggtgtcct
     3541 gtatgaggaa atacatattg atctacggaa atagtcgtcg tacgaatttt ctgtatttcg
     3601 acaatgttcg attgtcgata gtcagccgct acgattgctt tcggcctgtg gtgttgaaaa
     3661 acttgagcgc cgtcctcggg tcgcctcggc taaaagttga acttatggaa ctgagctgcg
     3721 acatcacttg cacggttaca ggttgaacaa acttcatcag ccaggtcccc aataatccgg
     3781 ggtgcttcag gatgcgtctt cggcaatttc cagctgaatg cctttgcagg cgtgggacaa
     3841 gtcgtccgtc aagggacgat ccgtgaccag aacttgaaca tccgaccagg gcaggctggt
     3901 gatgagacca ggggcttcga acttgctggc gtcggctacc acgatcaccg tgcccgcgtg
     3961 cgggcagacc gcctgcttga actcgacttc gaacgggtcg aagtccaggc aacctccacg
     4021 cgcgctgagg gccgccacgg agaccaccag aaagtcgggg tgcagctggg tggcgaagcg
     4081 cagcgcattg gcacccgcaa gggtcatatc actgcttcgc agagtgccgc cgggcagcat
     4141 cacggtgtgg cggtcgccgc tttgagccaa tgcccgtgcc acttcgagcg agggtgtggc
     4201 cacggtcagt ccacggcgct cggccagcgc ttcggccacg aaacaggcgg tgctgctgtt
     4261 gtccagcaca atgctggcgc cgtccggcag gagttcggac acccgccgcg ccagccggcg
     4321 cttggcggcg acgttgctgc gcaggcgcaa ctggaagggt gattcatgga catcgcgatg
     4381 gagactgaca ccgccatgaa acttctccac gatgccgtcg tccgccatcc cctgcaggtc
     4441 acgccgcacc gtctcgcccg acactcccag gcgatttgcc aggtccacgg tggtgatgga
     4501 aggggtggcc cgcaggatct cgacgatgcg ctcctggcgc gcgctcaaca ccggtgtcat
     4561 gttcatcgcg ttactcccgt gccgccagcg tggcgccggt ctgcccgtca aacacgcgca
     4621 ggcgggagag ctcaggtgtc agaaagacgg gtttgctggc ttccagtgcc gcatcggcct
     4681 cggtacgcac cagcacgtcg gcggtgccca cgcgtatctg cgcgtaggcc tcggcgccca
     4741 ggcgttccac ccgctgtacc acgcccggcc agctccatgg tgccgccacc tccacgctgg
     4801 agataagcag ctcttccggc cgcaggccca tcacggtgcc gggctgcaac gaggcgaccc
     4861 cgtagtgctg attgagccag gctcgcccct cggccatatc ttcagacacc ggcagaaagt
     4921 tcatttcggg tgacccgatg aatgaagcga caaaacggtt ggctggccgt cggtagacct
     4981 ccatcggcgc gcctacttgc cgcaggtgcc cacggtccat cacggcgatg cgcgtgccca
     5041 gggtcatggc ttccacctgg tcgtgcgtca catagacgat ggtgataccc agctcggcat
     5101 gcaggcgttc gagctcgccg cgcatgtggg tgcgcagttt ggcatccagg ttggacagcg
     5161 gctcgtcgaa cagaaagacc ttgggcttgc gcaccagggc acgcccaatc gccacgcgct
     5221 ggcgctggcc gccggagagc tcgttgggtc ggcgctgcag cagctcggtg atctgcagcc
     5281 gatttgcggc ctcgttcacg cgttggtcta cttcgctgcg cgtgagcttg ctggcgcgta
     5341 gcaggccgaa ggccatgttc atgtagaccg tcatgtgcgg gtacagcgcg taggactgga
     5401 aaaccatggc gatgtcgcgc tcgtgcggct cggcgtgggt gacatcgcga ccaccgatca
     5461 gcagccggcc ttcgctgatg tcttccagac cggcaatcat gcgcagggtg gtggacttgc
     5521 cacaaccgga ggggccaacc agcaccatga attcgcccgc ctgcacgtcg aggtcgagtc
     5581 gatccactgc aacatggtcg ggataacgtt tgctgatgcc ctggagggag atggcggtca
     5641 taactgggga tggtaagggc aagtggaagg ggtctacttc aggccggcgt gcatgaagct
     5701 gtcaacgaag cggcgctgga agatcaggaa cagcagcatc agcggcgcaa tgaccaccat
     5761 ggtggcggcc atcaggcgcg gccagtcggc accgctgtcg gccttggcca gcagccccag
     5821 cccgatcggc agggtgcgca ccgactcgct gttggtgacc agcagcggcc acatgtagtc
     5881 gttccagtgc gtcaccaccg agatcatgcc gaacgcaatc aacgtgggtt tcaccagcgg
     5941 caggtagacg tgccacagcg tcgcccagtg gccgcagcca tccaggcgcg cggcgtcggc
     6001 caggtcttgc ggcacctggc ggaaggcctg gcgcagcatg aaggtgccgt agccgctggc
     6061 gatgaagggc accatcagcg cccacaggct gttgagcaag cccagctcgc gcacggtgat
     6121 gaagttggtg agaaaggtgg cgtagatcgg gaacatcacc tgcagcagga acaggccgaa
     6181 caacaagtcg cgccccggga agcgcagccg ggcgaacgcg taggctgcca acgtgatcgt
     6241 ggtcagctgc gcgatcagga tgccgccggt gaccatcagc gagttgatga ggtaggtgcc
     6301 gaagggcgcg accgccaggg ccgccgggta gttgccccac tgcagcacct gcggcagcac
     6361 accggcggcg gggtcgaaga tttcggcgcg ggttttgaac gaggtgacca gcatccacgc
     6421 cagcgggctg agccacagca gcatggccgg cagcagcagc cagtgcatcc agcgcgaacg
     6481 ggcgaagaaa aatcgggtgc ccctcatctt cagcgcccca tgtagtgaac gcggcggccg
     6541 agtgtcagcg ccacgatggc aatcagcccg atgacgacca gcagcaggat gttggcgatc
     6601 gccgaggcgg tgccgatgtc ctggaactgg aaggcattct ggtagatgta gaacgtgagc
     6661 acgttggtcg cgtcggcggg cccgccctgc gtcatcacga acacgtagtc aaaaatctgg
     6721 tacgagtgca gaaccccgat gatcaccacg aagtacagcg tgggcgagat cagcggtacg
     6781 gtgatgtgaa tcaactgccg ccagccggaa acgccgtcca gccgggcggc ctcgtacagg
     6841 tcgcggggca ccagttgcag cgccgccagc aggatcagca tgaagtaccc ggcgtacttc
     6901 cagatgctca tgaagatgat ggctggcagg gcccactggc ggtcgtacag ccattccagc
     6961 ttgggcaggc ccaacatctc catcaggccg ttgatggggc cgtagcccgg ggcgtacagg
     7021 aacacccaca ccatgccggc ggccaccgaa ggcaccatca ccgggtagaa gaaaacggag
     7081 cggtagagcc cggtgccgcg cagtttgcgg tccagcgcca cggccagcag cagcgccaga
     7141 ccgatcgacg tgggtaccgt gatcaccatg tagaccacgg tgttgcgcag cgattgccag
     7201 aacagcgcgt cgtgccacaa gcgctgatag ttttcccagc cgacgaagaa gatcgcggac
     7261 gaaccgagct gcacgtcgta gaagccgtag atgagcgacc ggatgatcgg ccagtaggtg
     7321 aaagccgcca ggaacagcag cgacggcgcg agcatcacca ccgcggtgat gacctcgcga
     7381 cgacgcgccc gcttcctgac gccaggcacg gtttccagtg cagcggccag ggtcagtgtg
     7441 gtcatggcgt cgtcggtccc tcagctgcgc ctgcgggaca ggcgctggat ctcaaggttg
     7501 gatttcgcgg tggcctcgcg cagcgcggcg tcgggcttca cctcgccagc cagcgtgcgg
     7561 tcgatcgcgc tctgcaggtg cgcgcgcacc ttgtggtagc ccggcacctg caggaaggcg
     7621 ccagccacct ctgcggtcac gaaagcagtg cgcgctgcgg gcaccttggc cacgtgcgcc
     7681 ttgagcagcg gtgtttccca cgaggacttg cgcgcggcca ggtagccggt gtcgatgcac
     7741 cactgggcct ggttctgggt gttggtcatc cagcgggcga aggtccaggc cgcccgcttg
     7801 tgggcgtcgg gttgtttctt cgcgatcagg atcggaccgc cacccatggt ggagccgagc
     7861 tgcttgccct tgggcatggg cgcaacgccc accgggaacg aggcggactt gaggatgttg
     7921 gtcatcgagc cggtggagtg gtagagcatg gcggtgcggc cagccatgaa gtcgttggcc
     7981 gagctttccc aggtggaggc cgcgggcatc agtctggcct gcgccatgcg ctgccagaac
     8041 tccagcgcgt tcacgctttc cggcgcatcg aaggcgacct tgtccttggt ccagaatacc
     8101 aggccgttct ggcgcacaaa gctctcgaag atccagtcgt gccagccgcc gcctatggtc
     8161 agcccccatt gcgaaaccgt gtcgccctgg cgcaccgaga gggccttggc ggcggcctcc
     8221 agctcggtcc agttggtcgg cgcggctttg atgccggcct tgtcaaaggc gttcttgttg
     8281 aagtacagca ccggcgtgct gcactggaac ggcagtccat acagcttgtt gtcggacatg
     8341 caggtgccca ggaagccggg caggaaatcc tggaacacgt cgtccgcctt ggccaacgcg
     8401 gtcacgtcct ccagcgcatc catgtccagg aaggtgcgga gcatcgagtt gatgggcaac
     8461 cagctggcgg gcggctcgcc gacgcccagc gcggtcatca ccttttgctc ggtgttgtcg
     8521 tagctgcccg cgtagatcgc ttcgacctgg atgttgctgt gggcggcatt gaaactgctg
     8581 at
//
LOCUS       DAIDFG010000119         8527 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_119_length_8527_cov_15.503423, whole genome shotgun sequence.
ACCESSION   DAIDFG010000119 DAIDFG010000000
VERSION     DAIDFG010000119.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 8527)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8527)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8527
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119_length_8527_cov_15.503423"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1708 a         2655 c         2629 g         1535 t
ORIGIN      
        1 tgcagatcga aacccagggc ggcgacaagt tcttcggcga gcccatgctc gacatcagcg
       61 acttcatgca gacagttgac ggcaaaggcg tggtcaacat cctggcggcc gacaagctca
      121 tgaactcgcc gcgcctgtac gcgacctttc tgctgtggat gctgtccgag ctgttcgagc
      181 agctgcccga gatcggcgac cccgacaagc ccaagctggt gtttttcttc gacgaggctc
      241 atctgctgtt cgacgaagcg cccaaggcgc tgatcgagcg tatcgagctg gtcgtgcgcc
      301 tggtgcgttc caagggcgtc ggggtctatt tcgtcacgca gaacccgctg gacattcccg
      361 actcggtgct ggcgcagctg ggcaaccggg tgcagcacgc actgcgcgcc tacaccccgc
      421 gcgaccagaa agccgtcaag gccacggcgc agaccatgcg gcaaaagccc ggcctggaca
      481 tcgagaccgc catcaccgag ctggccgtgg gcgaggccct ggtgagtttt ctcgacagca
      541 agggccgccc cagcgtcacc gaacgcgcct atgtgctgcc gccgggcagc cagatcggcc
      601 cgatcacgcc gcagcagcgc caggccctga tgcagggttc gctggtagcc ggggtgtatg
      661 aaaaacacgt ggaccgcgaa tcggcccacg agatgctcaa ggagcgtgcc gaagttgcca
      721 cggccgaggc cgccaaagcc gccggcggcg gcgcggccgg gcaatcggct tccggcggca
      781 ttctgggcgg cctcaacgac gtgttgttcg gcagcaccgg cccgcgcggc gggcggcgcg
      841 agggcctggc cgaaagcatg gccaagtcgg ccgtgcgcac catgggctcc acggtgggcc
      901 gtgagatcat ccgcggcgtg ctgggcggcc ttttcggcag caagaagcgc tgagcctttt
      961 acctttcaac caaccagaca acagaaaccc aaggtattta tgagcaaggt tcacctgatc
     1021 gaccacccgc tggtgcagca caagctgacc ctgatgcgcc gcaaggacgc ttccaccaac
     1081 acctttcgca ccctgctcaa cgagctcagc agcctgatgg cctatgaagt cacccgcgac
     1141 atgccggtgc aggacgtcga gatcgagacg ccgctggaaa ccaccaccgg caccatgatc
     1201 gacggcaaga agctggtgtt tgtgtccatc ctgcgtgccg gcaacggcat cctggaaggc
     1261 atgctcaacg tggtgcctgg cgcccgcgtc ggccacgtcg ggctgtaccg cgacccgaaa
     1321 accctggagc cggtcgagta ctacttcaag atgccgcaag agatggaaga gcgcgacatc
     1381 gtgatcgtcg accccatgct ggccaccggc aactcggcca ttgccgccgt gagccggctc
     1441 aaaaagctca aacccaagtc catcaagttc gtctgcctgc tgacctgccc cgaaggcatt
     1501 gccgccatgc agaaagccca ccccgacgtg gacatctaca ccgcggcgat tgaccgccag
     1561 ctcgacgaac acggctacat cctgcctggc ctgggtgatg cgggtgaccg catctttgga
     1621 acaaaatagg cctccagccc aataaatacg ggcgtaagca gctacatttt taatagcaaa
     1681 ttcatgtttt atgagtctgc tgggcctgcc cccggccttc ccgcttgggg gcgatgaagc
     1741 cgaaccgcaa gggagaacgt tgatgcgcag ccgcccccag gacaaagccg acccggcttt
     1801 cctcgatgaa aaaaccgcac gcaaacaggc ggaacggcag gttttgctgg acgccttgag
     1861 cggcggcctg gaggctgaag ccatcgaatg gacagccggc agcgccatcg cacccgcact
     1921 gctggccagc tcgcgcatcc agctgcgccc gtggaaagac gccgaccgtg agccctttgc
     1981 cgccatggtg gccgaccctg aagtcatgcg tcactttccg gcaacgctga cccgcgaaga
     2041 aagcgacgcc tgggtgggcc gggccatgca aaagatcaac gagcgcggct gggggttctg
     2101 ggccatcgac tacctggctg acggggcacc ggcgcccacc ttcgccggct ttaccggcct
     2161 caacattccc gaccctgaac tgcctttcgg cccctgcgtg gaagtgggct ggcggctcgc
     2221 gccgcccttc tggggcctgg gcctggccac cgaggccgcg cgccttgccg tgcgggccgg
     2281 cttcgagtcg gtgggcctag aagagatcgt cgcggtaacg acgctgcgca acacccgctc
     2341 gcaagccgtc atgcggcgcc tgggcatgca ggaaagtgtt gccgacgaat tcgaccacta
     2401 tgccttcccg gctgacagcc ccgagaggcg ctgctgcgtg taccgcttgt cccgcgcgcg
     2461 ctggtctgcc aacagcctgg ccgggaacct tcagaaccaa agctgaaagg cgcaaggaat
     2521 gatggatctc ctgattacca acgccgccct gcccgacggc cgcaccggca tgtcgattgc
     2581 cgtgcaaaac ggcaagatca ccgaagtgac tgaaggaccg atcgccggcg cgcaggcgca
     2641 tgaaacagtc gacgccggcg gctacctgct gagcccgccc tttgtcgacc cgcatttcca
     2701 catggacgcc acgctgagct acggcctgcc gcgtgtcaat gaaagcggca cgctgctcga
     2761 aggcattgcc gtgtggggcg aactgaaacc cacactcaag ccagaggcca tcatcgaacg
     2821 cgccctgacc tactgcgact gggccgtggc caagggcctg ctcgccatcc gcagccacgt
     2881 ggacacgagt gatgccagcc tgctggccgt ggacgcgctg ctggaagtga aaaaacaggt
     2941 ggcaccctac atcgacctgc agctcgtcgc cttcccgcaa gacggcgtgt tgcgcagcaa
     3001 gggcggtgtg gagaatctga agcgtgcact cgacaagggc gtggatgtgg tcggcggcat
     3061 tccccacttc gagcgcacca tggccgaagg cgcggccagt gtgaagcttc tttgcgaaat
     3121 cgccgccgaa cgcggcctgc cggtcgacat gcattgcgac gagtccgacg acccgctgag
     3181 ccgccacatc gaaaccctgg ccttcgaggc gcaacgcctg ggcttgcagg gccgcgtcaa
     3241 cggcagccac tgtacctcca tgcacagcat ggacaactac tacgtgagca aactgattcc
     3301 gctgattgcc gagagcggcg tcagcgtgat cgccaacccg ctgatcaaca tcaccttgca
     3361 aggccgccac gacacctacc ccaaacgccg aggcatgacc cgcgtgcccg aactgatggc
     3421 cgccggtgtg aatgtcgcct tcggccacga ctgcgtgatg gacccctggt acggcatggg
     3481 ctcgggcgac atgctggaag tcgcacacat gggcctgcat gtggcgcaga tgacgagcca
     3541 gcaagggatt cgtgcctgtt ttgatgcggt gaccgtcaac gcggcgaagg tgatgcacct
     3601 ggaaggctat ggcatcgcac cggggtgtga tgcgagcttt gtgttgctgc aggcgcggga
     3661 tgtggtggag gcgattcggt tgagggctaa tcggttgaag gtttggagga agggcgtcct
     3721 actcgcggag acactcaaag tcacggttgc tttacggctg acacaacggc catcgacaac
     3781 ctgttttgtg acttgacgtc gggccgggca ctgtccggta gcccgccttc cgggccctta
     3841 ttcatgaaag gcagatagcc tccggcagag cgacctgccg acgacacatc taatttcctc
     3901 atcgaaggac cgtaaatatg acacttaaca tcgtccaaca acgtcttgac ggcctgctca
     3961 gttcctcgaa gcctaaggtt gtgcttctga ctgggggatg gggtgcagga aaaacatttc
     4021 agtggaagca gtccctcaaa aaagctgcgg ccgaccataa caaaccaagg tatgcctatg
     4081 tgtcgctatt cggactaagc agtttggcag agattcgaaa acgagtggca gaggaaatcg
     4141 tttcccaagt gacacttcca ggcaatagcg gaacggtggg cgaagtaatc gaaagagaag
     4201 gttatgcgct gaagccgatg cagttactca agctccttcc agctattcca gtgttaggaa
     4261 aacttgaaag tttggcgaac gaattgtcgt tctctgcggt acgtaaggcc gtaatctgtt
     4321 tcgacgactt ggagcgcgca gctccttccc tgaggctcgc cgatgtattt ggcctagcct
     4381 cgttcctgaa ggaggagaga gactgtcgag tgcttctaat ttcagatcaa gataagctca
     4441 atggcgaaaa gaaggacgac ttgacgctct acactgaaaa ggtcgttgac gagctaattc
     4501 actttgcacc agccgctcag acagcgtgtg agattgcttt gggcgagcaa cctacggcag
     4561 tgcgcctact tctgcaggaa agaatgatat tccttggcgt gtccaacatc cgggttatcg
     4621 ctcgacttgc ggcgatggcc gacgagttgc aagcactcct ggccggactg catgaagctg
     4681 taatcaagga agttgttcac tccttggcct tgtttggggc agcatacttt ttgccaaagg
     4741 atggatttcc acctccgagc ttcattcgag aatatgacac ggactggagg cactacctga
     4801 agagtgatga ccaaacgacg caaacagagg aagaaaaact gcaaagtgtc tggcgagaaa
     4861 aacttggcag ctacggatat tccgagacat cggcactgga tacagtgatt tcccatggcg
     4921 tcgagcgggg ctattttgat caagccaccg tattgaagct cgccaccgaa ttgtcgggaa
     4981 ccgctgaaga gttagcgcaa cgcgcgactt attcgaacag ctggacaaaa ttttggcact
     5041 cagcgagcgg cgacagcgca gcacttctac aaagtcttcg cgtcatcacc gacgattcgc
     5101 ttcgagtgat tagcgcgaat gacctgtaca acgcttgcga agttttcaag gaagcgtctc
     5161 aagaagatgc ggccaacgcg cttttggaaa aattcattgc tgtcaatcag tcccgaccag
     5221 gagttttctc agacgcggat ggccctttcg ggcacattta taacgaggcg ttcgcacaaa
     5281 gacttaaggc ggaagccatc aaacatgcgc agatttccag tgcacaggaa gcgttagatg
     5341 cgatcgattt ttccagcgga tggaattcca aagacgttac gcgggtctct gaagttgatt
     5401 tctctgagct agaagcgctt cttttggcat ccgaaggccg agttttcact aaacgactaa
     5461 aaacccttct gaacatcgga accggatcag atgccagtag caaagaaaag caagtaagag
     5521 aaaacacaat cgattggctt cgaaagttga caacttccga tccaatcctg gctattcgac
     5581 tgcgcaggtt tatcccgacc cggtaagggt gcaccggaac ttttgttgca tttctcctcg
     5641 ttcctgcgtt cccaatagaa ggcgcgggcc agcacagcgc gtttgcatat cgctcgtcaa
     5701 attggagagt tcagcaggta cagtgacctt accaaccaat ctccatggac cccacccacc
     5761 tccaccaact cgctgtcgaa gccatgcgca cccgcggcct cttgcccgag ttctctccgc
     5821 aggcattgct ggaagcagag gccgcgcgcc agaccggccc cgaacgcaat ggcgacatcc
     5881 gcgacctgcg gcacctgacc tggttctcga tcgacaacga cgacacgcga gacctcgacc
     5941 agctgagcgt ggccgagccg ctgcctgcgg gcgctgcgca cctgctggtg gcggtggccg
     6001 atgtcgattg cctggtgcgg cccggtggcg cggtcgatga ccatgcgggt gccaacacca
     6061 cctcggtgta cacggctgcg ggcattttcc ccatgctgcc cgaggtgttg tccaccgacc
     6121 tgacctcgct gcacgaaagc caggagcggc tggcggtggt agtggacatg caggtggccg
     6181 cagacggcac ggtgtcgggg tcagacatct accgcgctgt tgtgttgaac cacgcgaagc
     6241 tcacgtacga cgcggtgtcg gcctggctgg gcggcgacgg gccagcgcct ccgcaaatgg
     6301 ccagcgtgcc gggcctcgaa gcgcaggtgc gcctgcacga caccctggct ggcaagctgc
     6361 ggcagtggcg ccagtcgcgc ggtgcgctca atgtcaacac catttccgcg cggccggtgt
     6421 tcaaggacgg ccagctggtc gacctgcagc ctgacgaaaa aaaccgcgcc aaggatttga
     6481 ttgccgacct gatgattgcg accaacggtg cgacggcgcg ttttctggtg gatcagggtt
     6541 tcccgtcgat acgccggttt ttgcaggcac cgcgccgttg ggaccgcatc gtggccctgg
     6601 ccgcaagcca tgacgtgcag ttacccgaac agcccgatgc actggcgctg gaccgcttcc
     6661 tgagcgcccg gcgcgcggcc gacccggccg gtttttccga cctgtcgctg gcggtggtga
     6721 agatgctggg ctcgggtgaa tacgttgctg caccggcggt ggccggaggc acagccacag
     6781 caggaggcaa tggacagggc cacttcgggc tggccatcaa cgactacgcg cattcgaccg
     6841 cgccgaaccg ccgctttcct gacctggtga cgcagcggct gctgaaggcg gcgctggccg
     6901 gcgagccgcc gccttattcc gccgatcagc ttgacgagat tgcccggcat tgcacgctgc
     6961 aggaagacaa cgccagcaag gtcgagcgcc aggtgctcaa ggccgccggc gcgtatttgc
     7021 tgcacgaccg tatcggcgat gtgttcgacg ccctcgtgac gggcgcggcg gccaagggca
     7081 cgtttgtgcg catcagcagc cctttgctgg agggccgggt cgtgcggggt tttgaagggc
     7141 tggacgtggg cgacacggca cgcgtcaggc tgttggcggt ggatgcggag aagagcttca
     7201 tcgacttcga gcgcgcctga gcgtcattgc cgacctggcg gggaatgtaa cgccctgtga
     7261 agactcttgt agcgggggca agcagcgcca tctgggtcaa agcggcccgc cggccttgcc
     7321 cctcaccacc tcactcaagg agcttccatg ttcaaacgcc tgcccctgct gtgggttctg
     7381 ttgcgcggcg acgcccggca actgtggttc gccttgcgcc acccgcaggc gccgggctgg
     7441 ctcaagtggg gcacggcggc gattgcgctc tacctgatct cgccgatcga cttcatcccg
     7501 gacatgctgc ctttttttgg gattgccgat gacctggtgc tggtgccgct ggccatccgc
     7561 tggctgctca aacgcctgcc gccggaaatc gccgccgcaa ccacggcaca acgcagcgcc
     7621 ggctgagcgc cgccgcgctg gtgtaatggg cggtcgcccc aagaggctca accgctggcc
     7681 atcgccaggc ttccgtttat gcccacccgc tcctcttcgc tgaccatccc cctgccctca
     7741 ggcggcacca cctctggcct gctgcaggtc ccggtcaagg ccgaagcctg ttatgtgttc
     7801 gcccacggtg ccggcgcggg catggaccac gccttcatgg cagccatcgc gcaggggctg
     7861 gcggagcgcg gcatcgccac cttgcgcttc aactttccgt acatggaaca aggctccaaa
     7921 cgcccggacc tgccggccgt ggcgcacgag gcgattcggg cagcggtggc ggaagcgtcg
     7981 cggcaactgc ccaaggtgcc gctgtttgcc ggcggcaagt ctttcggcgg ccgcatgacg
     8041 acgcaggcgc aggcgcttga gccgctgccc ggtgtacagg gcattgtgct ggtgggcttt
     8101 ccgctgcacc cggccggcaa gccctcgacc gaacgcgcgg cgcacctggc cggcgtcaag
     8161 ctgcccatgc tgtttttgca gggcacacgc gacggcctgg cggagaaggg cctcatcacg
     8221 cagaccacgg aaagtcttgg aaaacgcgcc accctgcaca tcgtcgaagg tgccgaccac
     8281 gcctttcatg tgctggtgcg ctcgggccgc aacgatgcgc aggtgcgcga ggaactgctc
     8341 gacaccatgg ctgcgtggat gaagaaacgc tgacttgcgg gcagccgctc acggcacacg
     8401 cacggaaacg cccggttcgc ccgcaagacg tcgctgcgcg gcggtaaaca gccgaccgcg
     8461 gatcagctgc agcgcctgtc ctcggcgtga ggtctctggc gaagccgttg acgtggccgc
     8521 aatgacg
//
LOCUS       DAIDFG010000120         8514 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_120_length_8514_cov_18.236198, whole genome shotgun sequence.
ACCESSION   DAIDFG010000120 DAIDFG010000000
VERSION     DAIDFG010000120.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8514)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120_length_8514_cov_18.236198"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1386 a         2668 c         2824 g         1636 t
ORIGIN      
        1 agcgcaggtc gtcatacacg ccctcggcct tggcgatgcg tttgaggcgt tcgagttcgc
       61 gtttggcgcg gttcacgtcg gcaatgttgg tgcgctcgtg gctgagcagg tgtttggcgt
      121 aagtccagcc cttgttttcc tcgccgatca ggttctccgc aggcacttcg acgttgtcga
      181 accagacctc gttgacctcg gcctcgccgt cgagcagcac gatgggacgc acggtgacac
      241 cgggcgactt catgtcgatc agcaaaaagg aaatgccggt ttgcggcttg ccttcggtgc
      301 tggtgcgcac caggcagaag atccactcgc cgtactggcc cagcgtggtc caggtcttct
      361 ggccgttgac gatgtatttg tcaccgacac gttcggcccg gcatttgacc gaggccaggt
      421 ccgagcccga gcccggctcg ctgtaaccct ggctccacca gacttcgccg ctggcaatac
      481 cgggcagaaa gcgcttttgc tgttcggggc tgccgaacgc catgatcacc ggcgccacca
      541 tcaccgggcc aaacggcacg acgcggggcg caccggcgag ggcgcattct tcctcgaaca
      601 ggtgtttttg caccgcgttc cagcccgggc cgccaaattc cttgggccag ccgtggccga
      661 gccagccttt tttgccgagg attttggccc agcgctgcat gtcgtcgcgg ctcaaacgca
      721 gggcgttgtg caccttgtgc gaaatgtcgg caggcaggtt ggcgtgaacc caggcgcgaa
      781 tgtcttcgcg gaatgtctgt tcttcggggg tgaaagcgag atccataaga tgcgtgtctc
      841 cgtcgggttc gagttactgc tgcattttta gcacggtcgt tcggaatttt atgtcccagt
      901 tgggacaaat agcgagcgat tccgctttgc agaatcgggc gcgggcgagt gttttttccg
      961 gccccggata atcaccccac cttgccttcc taccgcacca tcatcagcgc cctcagtgtc
     1021 ggccagattc ttgtctgggc catcatgtat tacggctttt cttccttcgt gctgcccatg
     1081 cagcgcgaca tgggctggag tcagcccgac atcatggggg cgttcacgct gggcctgatg
     1141 gtctggggcc tggcgaccta tgcggtcggc gcggcgatcg accgcgggcg cggacgcgcc
     1201 gtgctgaccg gcggggcgct gctaggagcg gccggctgcg cgctctggtc gcaggcctac
     1261 agcctgccgc tgctgtatgg cgcctgggcg ctgctcgggt tggcgatggc gatgacgctg
     1321 tatgaaccgg ccttcatgga gatcaccaaa cgttttcccg accgctaccg ccagggcatc
     1381 accaccctca ccctggtcgg tggttttgcc agcacgctgt cgtttccggc ggtggcctgg
     1441 ctgcaggccc gcctgggctg gcgaccggcg ctgctggtga tggcgctgac ccttctgatg
     1501 gtggcgccgc tgcatgcttg ggtgctgcgc ggcggctgga agcggccgcc gctgcccagc
     1561 gccgcgcccg tggtagcggt gcaggaccag gccacgctgc gccaggccat ggagggttcc
     1621 accttctggc tgctgacggc caccttcacg ctttacacct ttgcgctgtc gacggtatgg
     1681 gcgcacctga tgccggcctt cgcgtcgcgc gggttgtcgc aggcagaggc ggtggccgtg
     1741 atggtgtggt tcggcccggc gcaggtggcg ggtcgctttg cctttctgtg gctgggccgc
     1801 tcgctgaacc accgcgcgct cgggctcgtg gtgctggcgc tgtttccgtt cgcgctgatg
     1861 ctgtttgcgc tgggccgcca gcccggggtg ctggtcgctt ttgcggtgct gttcggcgtg
     1921 gccaacgggc tgatcaccat cgtgcgcagc aacatcgtgc cggacttcta cggccgcgaa
     1981 catgtggggc gcatcagcgg cgcgatgtcg ggcattgcgc tgtgtacccg cgcggcggcg
     2041 cctttcctga cggcctgggc cttgctggcg ctgggcggct acaccgggct gctctggctg
     2101 ctggtcggca tgggcgtggc gacgctggcg ctgtttgccc tggccaggcc gccgcagccg
     2161 cgggcgtgac cgcgataatc cctgcattca tgcccaacct gatgccacgc cagtccaaaa
     2221 acatcgtgat cctgatctcg ggcagcggct cgaacatggc ggccatcgtc cgggccgcgc
     2281 aggcgcagca ttggcaacag cagttcggtg cccgggtggc tgcggtgatc agcaaccggc
     2341 ccgacgcggc cgggctgcag ctcgcgcagt cgctgggcgt ggccacgcag gtgctgagcc
     2401 accaggcgtt tgaatcgcgc gaagcttttg acgcggcgct gatggcactg atcgacacgc
     2461 accagcccgc cctggtggtg ctggccgggt tcatgcgcat tctcacgccg gggtttgtca
     2521 ggcattacgc gggacggctg gtcaacatcc acccgtcgtt gctgccggcc ttcaccggcc
     2581 tgaacacgca ccagcgcgcc atcgatgcgg gttgcctgga agccggctgc acggtgcacc
     2641 aggtgacggc cgagctcgac cacggcccca tcctcgcgca ggcggtggtg ccggtgctgc
     2701 cggacgacac ggcggacacg ctggccgcac gcgtgctggt gcaggagcac ctgctgtatc
     2761 cgcaagcggt gaaggcctgg ctgcagagcc agccctgaac cggcctggcg gtgtttcagc
     2821 ccgccttcaa ctgatgcagc gccgcctgga cgatgcttga tgcatcgacc ccgaaaaatc
     2881 cgcgcagcgc cgcacgggtg tcgctgcggc cgaaaccgtc cgtgcccaag gtcaggtagc
     2941 tgcggccggc cggcaggaag gcgcggatgc tttcgggcac ggcgcgtaca tagtcggtgg
     3001 cggcgatgat ggggccttcg ctgtccttga gttgttccgc aatgaagggc gcagacgcgg
     3061 gttcgccctg gagcgcgcgc tgctggcagg ccgcgccgtc acgcgcgagt tcgctccagc
     3121 tggtcacgct gtacaccgtc acgtccagac cctgctcggc cagctgctgc gccgccttga
     3181 tcacctcggt caaaatcgcg ccggagccca gcagcgtcaa tttttcaagc gtttgttggc
     3241 tctggccctt gtctggtgag cgcgagtagc tattgaattt atagcatccg cgaacaatac
     3301 cggcttcgga cccgggccgc aaatcgggtt gcgcatagtt ttcgttcatc agcgtgacgt
     3361 aataaaaaac gtcctgctgc tccaccatca tttcctgcat gccgcggtcg atgatgacgg
     3421 ccagctcgca ggcaaaggcc gggtcgtagg ccttgcagtt gggaatggtc gcggccacca
     3481 gatggctgga gccgtcctgg tgctgcaggc cttcgccgcc cagcgtggtg cggcccgagg
     3541 tggcgcccag cagaaagccg cgggcgcgct ggtcggcagc ggcccagatc tggtcgccca
     3601 cgcgctggaa accgaacatc gagtagtaga tgtagaaggg cagcatcgcc agtccgtgca
     3661 cgctgtagct ggtggcggcg gccgtccagc tggcaagtgc accggcttcg ctgatgcctt
     3721 cctcgagaat ctggccgtcg agcgcttcgc ggtaactgag caccgagccg atgtcttccg
     3781 gcgcgtactt ctggcccaca ctcgaataaa tgccgacctg cttgaacagg ttcgccatgc
     3841 cgaaggtgcg cgcttcgtca gcaacgatgg gcacgatgcg tgggccgagt gacgcgtctt
     3901 tcagcaggcc gcccagcatg cgcacaaagg ccatggtggt gctcatctcc ttgccgtcgg
     3961 cggccagtgc aaagttcgca tacacgggca gggcggggac agggacggtg tcggcgctgg
     4021 tttcgcgttt gggcagataa ccgcccagcg cggcgcgctt ggcgtgcagg tactgcattt
     4081 ccgggctgtc tgcggccggc ttgtaaaacg ccagttgctt cgcctgttcg tcgctgagcg
     4141 gcaggttgaa gcggttgcgg aattcgagca gatcgctttc gtcgaacttc ttctgtgaat
     4201 gcgtggtcat cttgccctgg ccggcgctgc ccatgccgta accttttttg gtgtgcgcca
     4261 ggatcaccgt cggctggccg gtgtggttgg cggcggcggc ataggccgcg tggattttca
     4321 cgaggtcgtg gccgccgcgc ttgagccggt cgatctgctc gtccgtcatg ccctgcgcca
     4381 gtcgtgcgag ctcttcgttc tggccgaaga agttgtcgcg gttgaagcgg ccgtctttcg
     4441 ctgcaaaggt ctgcatctgg ccgtccaccg tgtgcgcaaa ggcctgcgtc agcgcgccgg
     4501 tgatgtcgcg cgcaaacagg ccgtcccagt cgctgcccca caccagcttg atgacattcc
     4561 agccggcgcc ggcaaacagc ttctccagct cgtcgatgat gcggccgttg ccgcgcaccg
     4621 ggccgtccag gcgctgcagg ttgcagttga caacccagac caggttgtcc aggccttcgc
     4681 gtgcggccag cgtcagcgcg ctcatcgact cgggctcgtc catctcgccg tcgccgaaca
     4741 cgccccacac ctttcggccg gtgcagtcga gcaggcgtcg gtgcgtgagg tagtgcatga
     4801 aacgcgcgtg gtaaatcgaa ctgatcggac cgatccccat ggagccggtc ggaaactgcc
     4861 agaagtcggg catcaggtag gggtgcgggt agctcgacag tccctgtgcg ccttgggtcg
     4921 ccgccgagag ttcctggcgg taaaacccca gatctttttc gttcagccgg ctttcgaggt
     4981 aggcgcgggc atacacgccg ggcgcgctgt gcggctggaa aaaaaccagg tcgccgcgat
     5041 gctggttaac accgtcgatt tgaccgagcc cttcgcgtgc atgaaagaaa tggttgaagc
     5101 cggtttcaaa cagatcggcc gcgctggcat aactggcaat gtggccgccg agctcgctcg
     5161 atccacccga ctcggcctgg ttggcgcgca ccaccatggc cagcgcgttc cagcgcatca
     5221 acgaggccag ccgttcttcc atcgccaggt cgcccggaaa aaccggctgt tcgttgacac
     5281 cgacggtgtt gacataaggc gtggcgagtt ccggcttcca gctcacgcgc tgctcgcgcg
     5341 ccagccgcgc cagttcatcg agcaggtaac gtgcgcgggc ctggccttgc gtcgctgcca
     5401 gtgccatgaa agcctcgcgc cattcggcgg tttcctgggg atcggtgtcg ggtatgggca
     5461 tggcagcctg ctgcagggcg tggagggcat ggagtggggc gggcgcattc atgccctcaa
     5521 tataggccga ttgaccccct atgagctgtc aaaatacgtc gccataatcc tgtttggcgg
     5581 cataaaatgc ttcaaattgg agattttagg cgccatgaag ctggatgcac tggatttgcg
     5641 gatactcgac gagttgcagc gcgacggctc gttgtccaac gtggagctgg ccaggcgcgt
     5701 gcatttgagt ccgtccccct gcctggcccg ggtcaaggca ctggaggcgg ccagggtgat
     5761 cgaccgttat gtggcgatcg ccaatgccgc ggcgctgggg ctggggctga gcgtgttcat
     5821 ttccatcagc ctgcgctcgc aaaacaagga ctcgctcgcc gagttcgagc ggcgcattgc
     5881 cgagcacgac gaggtcatgg agtgttacct gatgacgggt gacagcgact acctgatccg
     5941 tgtggccgtg gccgacatcg cggcgctcga aaaatttatt ctcgagcagc tcacgccgat
     6001 tccgggcatt gaaaaaatac gctcgagctt tgcgctcaag caggtgcgct acaaaactgc
     6061 gctgccgctg ccagcgctgt gaagaggtcc ctagcggacg ggtgcctgct cttcgccggt
     6121 gagggctgga tgagaggttt cgagcgcatt gatttttgcc aggagatcct cccggcgtgt
     6181 gctgtcctcc ggcggaaggc tcgcaggttc gccggcagca tcgacaaact ggacgtagtg
     6241 cttcgggcct gaggtcattc gccgcaggcg ggttttgagg ctgtcgatga tttgctgtcc
     6301 ggtctgggcc cccggcgtgc cgtgccagat ggcgatgtgc aggttgaacg cgttggggtc
     6361 gtccagtttt ccggacaggc gtatgcagtc ggccaggatc ttggtggcga tttcggcgac
     6421 ggcctcgcga ttgaccgggc cttcgatgag catcagaaac aggttctcgt cgtaacggac
     6481 cactgtgtcc atgtcgcgcg cggcccggcg caggatttcg ccgatgcgcg aaagcgcggt
     6541 gctgcgcttg tggtcgctga gttcgtcagg gctggggacc tggtcgagcc agctcagcat
     6601 caacaggccg cattccgagt gtttgcggcg cgcgcgcagc agcatttgcg ccagccggtt
     6661 gtcgatcacc cggggcagca tcagcccgct ggccgggtca taagtggcca gggcggcacc
     6721 gcgatgggtg gaaaacaggg cagcccggct gcgccatgcc agcacaaaaa agatccacag
     6781 cagcccgacc atggccgaca acacgccggc cgcctgtgcc tcctcgatgt tctgcaccca
     6841 gcccgcgttg taggacagcc gcgccagcgc tgccagtgcg atgggtgccg tgcccagcag
     6901 gagcagcagg ttccatgcct gcccgcgcag ggacatccac accaggctgc ccaggattgc
     6961 caccgtggcc agcgccagcc acaggttgcg aaggtcgcgc gggatgaagt cccgatcgat
     7021 cgccgccagg ccggccacga gaaggctgcc cgctgcgacg gtggccagca gccggtaaat
     7081 gcgcgggtgg ccgcttttgg cgtagctggc ctgggcgcag aaccatgccc cggcggccat
     7141 gctcagcccg gccgagaccc agcccatgac ctggttgagg tgtgcgctgc ctggccacat
     7201 gcgccagccg ccgtagtcga tcagcaccag ctgcgtgaac atcagcgtca ccaccacggc
     7261 gccaaaccac aaaaacaccc ggttgcgtgc catggagaac gcggcaaggc tcagcgctgc
     7321 cagcacgctg aacatgcccc acatcaggcc aatcaccacg cccacgcggg aggcgccgtc
     7381 aacgtattcg acgggagaca gcagcatggg tcgccccgtg agcgcccggt tgttctcgaa
     7441 gcgcaggtag taggtctgca cgcggtcact gcgtgtctgc aattcaaagc tgggcacgcg
     7501 ggtgcgcaat gaccagttgg ccggcgcaat cgcctcgccg gccgcctgta cgagccagtc
     7561 gccccggggg ccgcgcgaga aaaggctggc cttgacgagc gcctggcccg gcaaacggat
     7621 gaaccaggtt tgaagcgact cggtggccgg tagtgagaag gtggcccaga cgtcctgctt
     7681 gcccgaagtg ggaagaactg tgttgtcggt gtagggctgc aatccccagg gcccggcggc
     7741 agtgaaggcg tcgggcgagg caatcgtgcc ctttggcacg gccacccact gcagcggttc
     7801 ggccagcgtg atggtgacgc tgtcgctgta aaggcggtca aagtcctgcg tgtggcctgc
     7861 ccgcagcacc aggccaagca gcaccagaaa aaaaatgcgc aagttggcct ttctgtacat
     7921 ccgcatagct tattgcataa cctgttgctt aagcgcgtgc tgtccggcag cggatcgccc
     7981 ggccatcacg atgtcacaag aattgacctc tatccgtctt ccttcaacaa cggatgccac
     8041 atgccttacc ccggcctcac cggagccccg acccgaacaa gcggacgcgc aagccgctga
     8101 acaatggcgt gccgaccttc ctcttcttca tgatcgaaaa cgtggacctg gtcgatgccg
     8161 cgtacatcat cgcgggcaat gacaacaagc tcccgttttc gggccgccgc gagccgaacc
     8221 aggctgacga ctacgcgctg atcctgctgg aagtcggcga tttccccaag gcgcagtgag
     8281 ggggcagggt gcaaatgcta ttgaatcaat agctgcttgc gcccgtccct tctgggttgg
     8341 aggccgaaaa gactagattt ttgactggaa gaccaggccc gcgtgttcgc gcagcgcatg
     8401 gaacttgatc ttgggccact gctgctcgac ggcgcgcaag gtggcgctgt gatcgaccag
     8461 cagcgtgggc gcatccaccg cgtcgagtgc cactcggtgc gcattggcgt agat
//
LOCUS       DAIDFG010000121         8420 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_121_length_8420_cov_16.775732, whole genome shotgun sequence.
ACCESSION   DAIDFG010000121 DAIDFG010000000
VERSION     DAIDFG010000121.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8420)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8420
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121_length_8420_cov_16.775732"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1380 a         2796 c         2689 g         1555 t
ORIGIN      
        1 gcccccagca cagccgcgcg cagccagccg atgcggtcgg tacggtgacg ttcaatatgg
       61 cgcgagaatt tcatgatcga gctggatggt ggagggtgga tggagcaagg gaaagcatag
      121 cccaggacca caccggctca ccgcctgcca tgggtacgcg ggctgtcgat ggcctgccgc
      181 aggctcttga gcagccgttg ctcgtcaatc ggtttttcaa gccagtcgga caccacaacc
      241 gtggcgcccg cgtgcagccg ggcctccgcg gccgtcaccg ataaaacgag gaccggcaga
      301 tctgtcgtgc ggtcttcctg gcgcagggag cggatcaatt ccagcccgtt ttcgtagggg
      361 agctggatgt ccacggtcat ggccgcgtag gactccatcc ggaggtagtc gcgggcctgc
      421 gctgccgtgt gggcgatgtc ggcatcaaag ccggccttgt ccagcatcat gccgatcagc
      481 tgggccacat ccgggtcgtc ttcacacacc aggatgcgcg gccggttgat gccgtaaaga
      541 ctcatgggcg ccgtgatcgg gggcgcctcg cgccactccg gcagctcgaa aaagaaggtg
      601 gttccgacgt ctactttgga agtgaagcca aggcttccat ccatccgttc aacaatcgct
      661 tttgaaatgg ccagaccgag gccggtcccg cccttctggc gtgtgtcgga cgagtcggcc
      721 tgcgagaact tctggaaaat gcgctggcgg aattcttcgg ggatacccgg tccgttgtca
      781 ctgacctcga cgcgcacacg gcccgcgtgc cggcccagca acaccagcac cgtgccctgc
      841 gcgggagaaa acttgatggc attggacagc aggttggtca tgacctgaag caggcggtcg
      901 ctgtccacca gggccttcag gggctgcctg ggcgcatgca gggccagctt gacctggtgc
      961 tgcgcggcaa aaccttcgtt gtccgccagc gtctgctgca gcaggggcag cagttcctgc
     1021 ggctgcagtt cgaactgcat cttgccggac tcgatttttt cactgtcgag gatgtcgttg
     1081 atcaggcgga tcagccgctc gcagttgttt ttggcgattt ccaccagact cttggcccgc
     1141 tccggcaacg cacccgccac gccccccgag accagcccca gcgagccgcg tatcgaggtc
     1201 aggggcgtgc gcagttcgtg gctgaccgtg gagacaaact cgctcttcat gcggtcgatg
     1261 cgtttgagtt cggtgacgtc ggtgcccagc gagaaaaatc cgatgacctt gtcctcctcg
     1321 ccttcttcac cgtagcgcgg aaaataactc atcacatatt cgcgccgctc gccgctggcg
     1381 gtgacctgct cccgttcgta acgcaccgca taaccggaca gcgcttcctg cacatggcca
     1441 ctgatctggc tgtaaaactc ggggcctgcg atttcaagca gtgttttgcc gtggattttt
     1501 tcccgcgaca agccaaacac ctcttcatag gccttgttgt gaaactggaa acgctgctcg
     1561 ctgtccacat aggccatcag ggccgggaca ttgtcggtca gctggcgcag ctgggcttcg
     1621 ctgtcgcgca acgcatcagc gatgtgtttg cgctcggaaa tatcgcggat gatggcggtg
     1681 aaaagccgcc gcccgcccac attgacgtcg ccgaaggaca cattgaccgg aaactcgccg
     1741 ccatcgcgac gcacggccat gacctcggta tcggactctt tgaagcctgg cggccggtcg
     1801 gccagggtgg cgaaaaaagc ggaaaaaccc ggctgccagc gcgccggcaa gagcatggcc
     1861 acaggctgcc ccagcaagtc cttgagccgg tagccgaaga ggtgccgggc cgcactgttg
     1921 gcgttgacaa ccaggccatc cggcgtcgcg gtgatgaccc catcggccat gctctcggtg
     1981 atggcacggc tcagggcttc gctgtcggca agctccgcct gcatgcgtcg gctgttggcc
     2041 tgtgcgcgga tcacggcgcg gatgctccag ccgagcagcc cgagcgagag caggccgctc
     2101 agcaggatca gcagccggac cgtctgcgct gtccgccggg cattctgctc ggaacgctcc
     2161 agggtcagca cctgcgcgct cttgatccgg ccagcgagtt cctcgatgtc gtcgtgcagc
     2221 tggcgattga ggtcgctgcc gaccagcgcg gtcgccgcgt ccagccccgc ctgctgccgg
     2281 gcgctgaccg ccctttccat gaggtccaga cgccgggcga tcagcccccg cagggtaccc
     2341 aggtcttcca cgctcgcgcc tgccgtcggc aggccactgg cgcccagcaa cacctgccgc
     2401 agtcccgccc cggctttccg gtaagccgcc aggtccttgt cgatgttggt caggaggtag
     2461 cgccgctgca gggactccgc ggtcttgaac tgcgaaacga gccgatccag cagaaccacc
     2521 gtcacctgga ttttttcttc gctttgctcg tcctccagca gcgcattgat ggtgaggtag
     2581 gacgtgaccg tgctcagtgc catcaggagc agacccaggc ccagggcaat ggtgatgttg
     2641 gtgcgaaagg gcagcttcat ggcggggcct catgcggggg cttggtacgg gagtcggtgc
     2701 cggagggctt ttcccgatcc gcctgtacgg acgatacacc agcggacccc gcacgccagc
     2761 ctgcggccga acgcagccgc gcgctcatgg cgtagccgcc gcaggcaagc cggcctccag
     2821 cgccgcgatc aattcgccca gcgcgccctc cagcggtttc agcaagccat cgagcatggc
     2881 gcgcggctgc cccccgtgca gggcctgctc cagcgcctgg gcatgttcgg gcacctgcgt
     2941 ggcgccaatc tgggcagaca ggccgcgcaa ggagtggacc agcagttcag ccgctttcag
     3001 gtcgcccgcg tccagcgcct cccggatgcg actgggcgtg tccctgcgtc cctccagaaa
     3061 gcggcccagc atggagaaat aaagcgtttt cacacccatc gcccggcgca gcccgagggc
     3121 caggtccagc ccggcgatga cggtgggcca ggcatcggcc ggcggctcac cagccgccgt
     3181 cgcttccagc ctggccagcg ccttgcgcag ctcgctcacg gcctcggcct tttgcgccac
     3241 ggtcaggcgg ccggcatcga tttccaggcg caggcccggc cggtcgcagc ggatgacctc
     3301 cggatcggca ggcattcccg tggcatccgg gcggaccaca tacacgggaa tgggttggga
     3361 aaaccccttg accacgatgg cgtcgcgcgc gtcggactgg acctcgccct gcaccagggc
     3421 gtgggtctca ctggaaatca ggatctcgcc gggctcggcg gcctgctcga gccgggccgc
     3481 caggttgacc tcgcggccga tgatggtgta ttccatgcgc aggctgctgc cgaaattgcc
     3541 gacgtcgcaa aaccccgagt tgatgccgat gcgtatctcg aaattcctgt ggatgcccat
     3601 gtcccgccag cgggcgcgca gcccttccat gcgtttttgc atggcaatcg ccatgcgcac
     3661 gcactgcacc gcatcctcgc gcacgcccag cgtatgcggg tcgccaaaaa agatgacgat
     3721 ggcgtccccg atgaatttgt ccagcgtggc gccgtattcg gcggcaatct gcgacatttc
     3781 cgcgaaatag ctgttgagca gcagcgtgac ctcttcaggc tgccactgcg ccgtggactc
     3841 ggtgaaattc ttgatgtcgg aaaaaaacac cgtcagcttc ttgcgctgcg tccggatttc
     3901 cgcctggcgt gaaccatcaa aaagcgactg gtaaacctgc ggcgccagat aacgcgagag
     3961 ctgctcggac agcacctgca agcgcctggc gttggcactg cgctccagat gcagcttcag
     4021 gcgcgccaga agcaccggcg ggctgatggg cttggtgacg tagtcggtgg cgcccaggtc
     4081 caggccccgg tgctcttcgt cctcgctgcc cagcgccgtc aggaaaatga ccggaatatc
     4141 ggcggttcgt gggtcccggc cgagccgggc cagcacctcg tagccgtcca tgtccggcat
     4201 caccacgtcg agcaggatga tgtcgggccg ctcggccgac cgggccatgt ccaggccggc
     4261 ttcgccggcg gctgccgccg tcacctcgta gctgacctgc aacagggccg tgagcaggta
     4321 acggatgtcc tcgctgtcct caatcaccag gatacggggc ctgtgcagcg gcgcaggcag
     4381 gttcgcacgg ctgctctgct tcagttcctc tgcttccggt ctgcccgcaa tctccatggt
     4441 ccggcccttc caatccagcc agggcacggc gcctggttca cccgcaggct gcgctatcgg
     4501 cctaggtcgc cagcgctgcc cgcatgcccg cgatcaccgc ccgatagtcc ggcttgccga
     4561 aaatcgcact gcctgccaca aaggtatcgg cgccggccgc agccacctgc gcaatgttgt
     4621 ccagcttgat gccgccgtcg acctccagac ggatgtcctt gcccgacgcg tcaatgcgct
     4681 tcctcgcctg ctcgatcttg cgcagggccg agtcgatgaa actctggccg ccaaaaccgg
     4741 ggttgacgct catgatcagg atcaggtcga tgtcgtcgat gacccagtcc agcacgtcca
     4801 gcggcgcggc cgggttgaac accagaccgg ccttgcagcc tttggacttg atggcctgca
     4861 cgctgcggtg cacgtggccg gaagcgtcgg gatggaagct gatcaggtcg gcgccggcat
     4921 cggcaaaggc cgcggccagg ctgtccaccg gctgcaccat caggtgcacg tcgatcggaa
     4981 cggcggtgcc gtcggccttt ttggcgtgcg gcttgagggc gctgcagatc atgggcccga
     5041 aagtcaggtt gggcacgtaa tggttgtcca tcacgtcaaa gtggatccag tcggcgccgg
     5101 cctcgatgac cgaccggagt tcctcaccca ggcgcgcgaa atcggcggac aaaatcgaag
     5161 gggcaatccg gtacaccggc ggcttgtttt ggctcatggc aggattgtcg cagcaagcgc
     5221 gcagcttgcc cactgacact gcaatttccc gtcctgcgaa gggcggttaa catgattgcc
     5281 actcttttcc aatccatgcc gaaatacgaa ttttcctgcg acgtcctgcc gcactacctt
     5341 cccgatcaat ctgcacccga cgacgaggtg tatggctttg cctacaccgt cacgatcacc
     5401 aacaccggcg aagtcgcggc gcagctgatc tcgcgccact ggatcatcag cgacgcacgc
     5461 ggccacaccg aggaggtcaa gggcctgggc gtggtgggcc atcagccgct gctcaagccg
     5521 ggcgagtcct tccagtacag cagcggttcg cgcctgcgca ccccgaccgg caccatgcat
     5581 ggcagctact tctgcgtcgc cgaggatggt gaacgtttcg agtcggtcat tccgatgttt
     5641 gtgctcgacg ctggcgatgc cggcggaccc ggcaacgggg ccggcggcgg ccgcgtgctg
     5701 cattagggcc ctctggtcat gcggacactg agcgagctgc tggaccagct ggagccgcgg
     5761 gccgaattgg ccgtgcggca cctctggctc agcgacctgc tggactggat acgcggagac
     5821 gccagcaatg cccaggcggc gctgtcgcgg gtggggctgt tcctcgacgc ggcgcaggcc
     5881 cggccgggtt ttccggagcg gctgcgtcta tggtggctga ccctggtcga aacggtggat
     5941 gccacgcccc tgtttgccga tttcggtttt gcgccgcgtg ccgcttttct cagcgaactg
     6001 ggcgaacggc tgcgctacaa gctgctgccg ggcacgcccg agaccacgca tgccaccgaa
     6061 ctgttcatgc tgctgttccc ggtgccgttt gacgcgcagt ggctcaaact gctcgacgag
     6121 ggcacgctgg ggcggctggg cgccctgctg gacccgccca tcgccgcacc ggccggcgac
     6181 gaagcccccg tcgaaccctt tgcccccgcc gccagcaccc tgccgcagga ttggcaaatc
     6241 accctgttgg actccctgac ctactgcacc agccaggtct gcgccaccgg ctttgcgccg
     6301 gaactgcgac tgcgcatgag cccccagagc ctgttggccc ggccctttca cggcctgcaa
     6361 accgatcttg aagcgctgcg cgccgcgctg ctgctgcacg ggcccgacag cggcgcgctg
     6421 cacctgacgc tggaccagtt caagagccgg ctggaggctt gccgcagtgc cgcctcgtcg
     6481 gtgtacgccc atctcgacga acacggcatc tcggtcggcc tggtgtttcg cctgcgccag
     6541 ctgcgcgaac gcatcctgcg cattcgtgag ctgctcgact gcctgctctc ggacaagccc
     6601 gccaccagcg ccatgcagct gctggcccgg ctggtgctgc tggccgaaga acggcgcagc
     6661 atacgcgcgc tggttgccag caactccacc ctgctggccg ccaaggtggc cgagcgcggc
     6721 gccgaaaccg gcgagcacta catcacgcgc gaccgtgcag cctataccaa catgctgggc
     6781 acggcggccg gcggcggcgc cgtgacggcc atcacgacgc tgctcaagtt catggtggtg
     6841 gggctggcgc tgtcggcctt ctggagcggc tttctggcca gcatcgtgta tgccgccagc
     6901 tttgtgctga tccagttgct gcacttcacg ctggccacca agcagccggc catgacggca
     6961 ccggccatgg ccgccaagct caaggacctg cgcaccggcg aagcggtcag cgagtttgtc
     7021 gacgaggtca cccacctggt gcgttcccag gtggcggctg tgctgggcaa cgtgcttgtg
     7081 gtggtgccgg tggtgctgct gatcagcctc gccatgcagt atgcgctggg gcagccgatg
     7141 atagacgcga aagaagccgg tcatgtgctg cacaccctga gcctgttcgg ccccacgctg
     7201 ctgtgggccg ccttcaccgg cgtagtgctg tttgtcgcca gcatcatagg cggctgggtg
     7261 gaaaactggt ttgtgctgta ccacctggcg tcggccatgc gttacaaccc gcgcatcacg
     7321 cgggcattgg gcgtggtacg ggcgcgacgc tgggcccatt tcatgcgcga caacatttcg
     7381 ggcttcacgt ccaacatcgc cctgggtttc atgctggggc tgattccgcc catgaccggc
     7441 tttttcgggc tggagctcga agtgcgccac gtcaccttgt cctccggcca gttggccgca
     7501 gccgcggcgg ccctcggctg ggatgcgctg cggcaaccgg ccctgtggtg gtgtgtggcg
     7561 tccatcccgc tgatcggtgc actcaacctg ggggtgagct tttacttcgc cttccggctg
     7621 gcgctgcgcg cccacagcgt cagcggtgtg gaccgccgcc gtatccgttc cgcgatctgg
     7681 gcccgctggc gcagcaagcc ggccagtttt tttctgcctg attgaatttt tcgggcagga
     7741 agtttgcaca ggaactgtca acaccctgac aacaggcgcg caaatcgcgc agcaagtccg
     7801 catgtcagtg attgtcctac gtgcaaagag tgtgaaacgt tgtaaattag gactcccttt
     7861 tccatgggtg cctgcccggc ctgcaaccac cggccgcaac aatgaaccac agcaggcccc
     7921 ggggcctgtc gccgcatcgg cggcttgtcc ccatcgagca cccgcgcatc ctggcgcggc
     7981 gctgcgtgaa tttcaatcaa tcagcggaga cactcatcca tgaatgaagt catggccatc
     8041 tgggccgaat ggatcaaacc ctcggcaggc ctgcccacag ttcagtggtc catcctgctg
     8101 gcgctggccg cgctgaccgg gcacctggtg cagcgttatg ccggcgttcc caaggtggtg
     8161 ggttattcgg cggtcggcgc ctttgccggc ctggccgggt tcaccggcgc ggcctggccg
     8221 ctgcagggaa cgggcctgtt tctgctggag ctcggcgtgg ccgtcgtgct gtttgaagca
     8281 ggcggccgca ttccgctgcg ctggttccgc cacaacccca tggtgctggt gcaaagcctg
     8341 gccgaatcga cgctgacctt tgttgccgtc tactgggcgc tgaccttttt cggcatgcgt
     8401 gaggttgtcg ccgagccgct
//
LOCUS       DAIDFG010000122         8416 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_122_length_8416_cov_16.128693, whole genome shotgun sequence.
ACCESSION   DAIDFG010000122 DAIDFG010000000
VERSION     DAIDFG010000122.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8416)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8416
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122_length_8416_cov_16.128693"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1716 a         2594 c         2525 g         1581 t
ORIGIN      
        1 ctggacctcg gcgcaggttg atgcccatgt acagaaatac gtggcgctgg tgggcctgtc
       61 ggccgccatc gacaagaaac cttcggccct gtcgggcggc atgaagcagc gcgtcggcat
      121 tgcgcgcgcc tttgcgatcg agcccaagat gctgctgctc gacgaaccct tcggcgcgct
      181 ggatgcactg acacgcggca ccatccagga cgagctgctg gccatcgtgc gtgaaacgca
      241 gcaaacggtg ttcatgatca cgcacgacgt ggacgaagcc gtgctgctgg ccgacaagat
      301 cctgctgatg agcaacggcc cgcaggcgcg tgtggccgag atcgtgcgca acaccatgcc
      361 gcgcgaccgc cagcgcgcca ccgtgcacca cgacccgcag tactacccgc tgcgcaacca
      421 cctggtggac ttcctggtgg cgcgctccaa agatttgtcg cacggcaagg cgccggcgca
      481 cccgcccacg gtggaaccgg gacgcggtgc actggccgac gcgccggcct ccgtcacgct
      541 gaaagccgtt gcatgagcac cgcgcaagcc cgcccgccgc tgccgccctt cacgctggaa
      601 agcgccaagg aaaaagtgca gaaggccgaa gacgcctgga acagctgcga cgccgaccgc
      661 gtggcgctgg cctacaccga agactcggtc tggcgcaacc gcgccgactt cttctcgggc
      721 cggcccatga tccgcgagtt tctcaaacgc aaatgggcca aggagctgga ctaccggctc
      781 aagaaggaac tctgggcttt caccggcaac cgcatctcgg tgcgcttcga gtacgagtgg
      841 cacgacgacg ccggcttctg gttccgctcc cacggcaacg agcagtggga gttcaacgag
      901 ctgggtctga tgcagcgccg cgaggccagc atcaacgacg tgcccatcaa acaagccgag
      961 cgcaaattcc actggccgcg ccctgtggcc ccctgatcac cccttttttt tcttttcaac
     1021 acggagaaca acaccatgaa ccgcaacgac atcaccgaaa aaatcatcac cgtcaaagtc
     1081 agcaagggca tcacctggga atcggtcgcc aaaaaggtcg gcctcagcaa ggagtggaca
     1141 accgccggct gcctgggcca gatgaccttt gacgaaaagc aggccaaggt ggttggcaag
     1201 attttcgggc tgacggcgga agagcaaaag tggctgcagg tcgtgcccta caagggctca
     1261 ctgcccacac ccgtgcccac cgacccgctg atctaccgct ggtacgaaat cgtgagtgtc
     1321 tacggtacga cgatcaagga actgatccac gaagagttcg gcgacggcat catgagcgcg
     1381 attgattttt cgatggacat cgtgcgccag ccagatccca agggggatcg cgtgaatgtg
     1441 gtgttgtcgg gtaagttttt gccgtacaag acttactgat tctggaccgg ggtgccgcct
     1501 ggcgccctgg cgtccctcgg agaagtcacg accggtacaa gaccaaacgt ctccggcatg
     1561 tcgaagtccg taaagtcatt ggtcttgaac cgatcaacaa gatgcgccct agccttgatt
     1621 ttgacgtccg cgagctttgc gtatgcacgt cgaagcgttt ctaggcagtt atccgtgcta
     1681 ttcactatgg ttccgaagag gtgtctaccg agaaccagct ctacggcgcc ttgcgtaacc
     1741 actccaccgt tgggaatgac cgatgcggca agtgccggct gtactccctc gtagtggagt
     1801 tcggagcgca tattccactg attcagtgct tcaacatagt tctcttgtgc cagccagaaa
     1861 tcgtgcataa cttcccgccc aaccttctcg tcaagcaaga agcccaaatc ggaaacctga
     1921 agcagattct tttccactca aacctcggag ttgctggaat ggaaatgaat cgacctgggt
     1981 tctcgagatg ctcaaagacg tagtcctttt gaatcaacac aatcgtattg atctgctgat
     2041 gcaaggcaaa catgagtctg tgagctgatg tcagcgcgtg cctctcgtca atccgtgcct
     2101 gcatacgacg ctgaaagata tatgcaagca ttgcaccaac gagtgcgccg acaagcgtag
     2161 ccagtccatt ggcgaaagcg tctggcgtga atggcttcaa tacgccggca aggtccgctg
     2221 cggaggcggt tgcagatgcg gcagccgtag aggcggcttg tgatgcggcg agaacttccg
     2281 acatgtcgtt atgaaggcta acgcagaagt gaccggcggg ccacaggcac gcaacagcgt
     2341 gccgcgagga cgtccgtgtc gtctgccacg ttagaccaga tttggggcga attcatgggg
     2401 aggtgataaa cctaatgaac ctcttgccgc ggtccgcagt tcgcgagcca atcatgcctc
     2461 cgccagtcat cgtcacgtgg agaaactgcc cttgcccaag caagccttcc gactgcaggt
     2521 cacgaacgat ctgatcataa gtttcgcgtt gtccgcgcaa atcaggaagg caatgctcca
     2581 aaaccgtgga aggccccccc gccccccagc cggggttcgc aacaccatgg agttccatcc
     2641 atcgaactgg atcgtccaac acggaaagga ctttcaggtg ccaaggggta agttggtcga
     2701 tcaatcgcaa gaagatcatc tgctcatctt cctgcggggg atttggaagc gctgcgttta
     2761 aaacggcgtt gcgcaaggct tcgagttttg cctgctggtg attccgcaaa gctatctgcg
     2821 acgcctgcat tgccaccgtc acgaatgctt cgttttgcgc cagcttctca ggcgtaagtt
     2881 ccgccacacg accctcaact tccttcacca cgtcggcgag ctgttgaagc cacgcctcct
     2941 ttctcctctc aattggagaa gtgaagaagt tctcgaacac aacctgcagc gagccgccag
     3001 caacgggcac gagcgataca actgcacgcc cgacttcgcg ggcgacatcg ccggcgctct
     3061 tttttgtagg taggtcgtcc ataagtgtga gatctaacgt tggaattgac cggcgaccgg
     3121 gaagcgtgcg cgagaagcgc actgaagcac ccggacgtcc gtgtcgaacg acctgctaga
     3181 cggcaggctc gtcatcgaca aaaatgcctc ctgaggccga atggagatgg tctgcttcag
     3241 ggtacccaaa cagcgcgcct aagtacacca tcaacgcatg aaaccggtcc ttggcactca
     3301 acacccactc gacattgagt gatttgtacc agtctggctg aagcttgtcg cccacaagca
     3361 tcttctcggg ttcgacatgg tccggaagct tcgggccgag cacacgttcg ggcttgccat
     3421 gggccagttt gtctcgcaag cgaaagactt cggtcgcgaa ctgcagcggg tcttggcccc
     3481 agtcggcctc gcgatttgca gtggtgtgaa gcacgcttac ctttgccttc caggggagcc
     3541 gctcaatttc gtcccaaaaa ggaatggctc gcgcaccaag agagttcagg tacgactcta
     3601 tcgagaacgc aaggaacacg accacaggag caagtgggag cttggcaggc tgttccttgt
     3661 ctagaagggc gtacatcttc atcagggcgc caacggacct gctttgcgcg gtgaaatcaa
     3721 agtacgcttg gtaggtcgcc acggtttccc ctgccctcta acgtaatgta tgtcgaataa
     3781 cctgcggtat aacttggcgg ccacggtatt acgtggacac acaatcctct tggaaaatcc
     3841 gccagcattc actttgctag aaattggtca aatttataca ggtataaatc aagccaccaa
     3901 acccggcagc aactgccaac gaattctagg cctccatagc gatcgagcca tccctcaatt
     3961 ggaggaagtg tggcttttgg cacccaccaa ccacgctgat cctgctgcca ctgcctccca
     4021 caccaaggca aaatgccccc atgaactccc tagacggcat ccgcatcctc gacctctccc
     4081 gcgtactcgc gggcccctgg tgtacccaga ccctggccga cctgggcgcc gacgtcatca
     4141 aaatcgaacg gccgggcacc ggtgacgaca cgcgcacctg ggggccgccg tttttgaaag
     4201 acgcgcaggg gcatgaaacg caggaggccg cctactacct gggtaccaac cgcaacaagc
     4261 gttcgctgac gtgcgacatc gcccaacccg agggtgcggg gctgatccgc gagctggtcg
     4321 cgcattgcga tgtgtttgtc gagaacttca aggtcggcga catggcgcgt tatgggctgg
     4381 actatgcctc gctcaaggcc atcaacccgc ggctggtgta ttgctcggtc accggctttg
     4441 gccagaccgg gccgtatgcg gagcgcgcgg ggtatgacta cgcgatccag ggcatgggcg
     4501 ggctgatgag tgtgacgggc gagcgcgacg acctgggcgg tgggccgcag aaggtgggcg
     4561 tggcggtgtc ggacctgatg accggcatgt atgcgacggt ggcgattctc gctgcgctgc
     4621 gccatgccga gaaaaccggc gagggccagc aggtggacat ggcgttgctg gacacgcagg
     4681 tagccatgct ggcgaacctg ggcgccaact acctggtcag cggcaaggtg ccggggcgcg
     4741 cgggcaatgc gcaccagaac atcgtgccct accaggtgtt cgaggtcgcg ccggctgcag
     4801 acggcaaaaa gcaggagggc gccgccgaag cggcgcggga tcatttgatt ctggcggtgg
     4861 gcaatgacag ccagtacgcg aagttctgca cggtggccaa catcccggag ctggcgacca
     4921 acccgctgtt cgccaagaac cgcgaccgcg tgctcaaccg cagcaagctg gtgccgattc
     4981 tggaagccgt gatgaaaacg cgtaccaagg ccgactggct ggcggcgctg gaggcggcca
     5041 aggtgccgtg cggcgcgatc aacaatttag ccgaggtgtt tgatgatccg caagtcgaag
     5101 cgcggcacat ggtcacagac tggcagcacc cggtaaaaaa tcacttgcgg ctggtgtcca
     5161 gcccgatcaa actcagtgtt acaccggtac gcaccgacct gccgccgccc atgctgggcc
     5221 agcacaccga ggaagtcttg cgcagcgtgt tgaactactc cgacgcgcaa ctgtctgacc
     5281 tgaaacacaa agaggtgatc tgatggccaa ttttgttctt gttcatggtg cctggcacgg
     5341 cggctggtgc tggcaacgcg tcaccctggc gctgcagcag cagggccacc gcgtgcacgc
     5401 ggtcacgctg accggcctgg gtgagcgcgc ccacctgctg gcgcccagca tcacgctgga
     5461 cacgcacatc gacgacgtga tcagcgccat cgaggtggaa gagctgcacg acgtgattct
     5521 ggcggtgcat tcctacgccg gcatgattgg cacggccgtg gccgaccgtc tcggcaaaca
     5581 cctcaagcac ctggtctacg ttgacgcggt ggtgcccaag cccggagaga gctggagcag
     5641 cacgcagtcg gcagcgacgc agcagcaacg cctgtcggcg gcgcaggcct cgacgcgttt
     5701 cagctttccg ccgcccgacc ccgaagtgtt tggcctgcat gacgccgacc acgcctgggt
     5761 caagcgccgg cagacgccgc accccggcaa cacttaccag gcgccgctgg actttgacat
     5821 caagcgtgtc gccgcgatcc cgcggacctt tgtcaactgc acacagccgg cgctggccac
     5881 catcgagccc agccgcctgc gcgtgaaaga ccccaaattc tgggacggcg cgtggttgcc
     5941 caactcgaag atcgtggagc tcaagaccgg ccacgacccc atgatcagcg accccgccgg
     6001 cttgacgcgc attcttctgg actgcgcggc atgatgcatt tacgcaggcg ccactcctct
     6061 gccgcgctgg caggcgggcg ggtactgcct gcgccggcgt gagcgacgag gctccccatg
     6121 aacgacgccg actccctgaa cgcggccatc cacgaggaag tggccttgca ggcctacgac
     6181 gccgcctggc cggacaggtt tgtggcggag cgggaccggc tgctggcgct gtttccgctc
     6241 gtttttgtcg acatcgaaca catcggaagc acggccgtgc ccgggctggt ggccaaaccc
     6301 gttgtggacc tgctggccgg tgtcgaaacg atggccatgg ccaaatcgct gaccgaacca
     6361 ctgtgcgaat ccggctacac cacgtcagcg gaattcaacg ccacgctgac agaccgccag
     6421 tggttcatgc gctgggccga cggacaccgc acgcaccacc tgcacattgt tgttcacggc
     6481 ggtgaggtgt ggacccggca tttgcgcttt cgcgatgcac tgcgcgccag ccccctgctt
     6541 gcagcgcgtt atgccgcact caaggccgaa ctggccgtcc ggcacccgac cgaccgcgaa
     6601 gcctatacca gcgccaagac cgcgtttgtg aactcgctgt cggaccccaa ctgagtgacg
     6661 cctcaggtgt gggcggaaaa atccgcggcc actccttttt tgatagcagc ttgcgcccgt
     6721 tgccattggg ctaggggctt tttttactta tagaagacgc ggccgacgca ttttgcgtag
     6781 ccgctaggcc ttcggccacg ggcgttaaag taaaaggggc agcgactgcc agagcggccg
     6841 tcgctgcccc gcctgcgggc gggctgccca cgggttcggg tcctgctggg gaacttagaa
     6901 gcgccagccc aggcccacac cgagcaggac cggatctatt ctgaattttc ccacgccggc
     6961 gccgcccgac aacacctccg tgcggatata gacttttttc acatcaaagt tcaggtacat
     7021 ctttccgccc agaggaatgt ccacgcccgc ctgcagggcc gggccccagc tgttgcggtc
     7081 cacgtccagg ccaggggaca ggttgactga actgaagcgt gtgtagttca ccccggcgcc
     7141 cacgtagggc ttgaagccgg tggattcaaa gtggtactgc gccatcagcg tgggcggcag
     7201 gtgcttgagc gtaccgaggg aggtgccgcg cgacgtcaag gtgtgttttt gcggcacggt
     7261 cagcaccagc tccaccgcaa cgttcttgtt gaagaagtag ctgatgtcca cctcgggcat
     7321 ccacttgtcg ttgagctcca ggcccaggcc ggtgctgtcg ccgttggtgc tgccgagatg
     7381 cacggctctg gcgcggacca tccaggggcc ttcggcgggc tgctgggcca tggcggtacc
     7441 cgccacgagg gaacacaaaa gggcggcggc aagaacttgt tttttcatgg caaacattgg
     7501 taagaacagg gaatattccc agttctcgat tgaatgcctt tggcacgtac gcaactttga
     7561 tgctgcgcag aagtgcttcc caacgccagc caccggatga cgagactttg ccgcagatca
     7621 aaaaatcatg cgccagccgc cccgtgcctg gcgatggcct gcgcgctact tcatggaccc
     7681 gaacaccttc ttcagaatcg cgctacccgt gcccaccggg tcctggcgaa tctttttttc
     7741 ttcctcgccg atgatcaggt agagcccgtc cagcgtcttg ccggtaacgt actgctggat
     7801 gttggcgtct tcctttttga ccaaaccaaa accggcggcc ttgccggcga actcgttgta
     7861 tttgttggcc agccccactt tggacgtggc ctgggtgacg accggcagga acttcacccc
     7921 cagcggggcg cgggttttct cggcaaagaa gttcgtgacc gaggtctcgc cgccggccag
     7981 gatgtttttg gcgtcggtga cattcatgga gcgcacggcg ctgaccagca ggtccttgcc
     8041 catgggcacg gcggcttcgg cggcgcggtt gatggacgtg accagctcgt cgatgcgctt
     8101 gccctggccg aagttcttca ggattttcga cgcgtcttcg aggtatccgg gcaaaggaat
     8161 gcgaactttc gggttgccga ggaatccatc agtcttgcca agcagggcca cggccgccaa
     8221 ggcgcccttt tcgagggcgg ttttcaggcc gatggacgct tctgcgctgc tcaggtcgcc
     8281 cagcgacagg gcctgcgcct gctggtaggt ggtcaggatc agcatgccca gtgcgctgag
     8341 accggcctgg ttaaaattgc gacgttgcat gacgagatcc ttccccatga gttcagtttc
     8401 aagcgcagta tgccgc
//
LOCUS       DAIDFG010000123         8364 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_123_length_8415_cov_18.165909, whole genome shotgun sequence.
ACCESSION   DAIDFG010000123 DAIDFG010000000
VERSION     DAIDFG010000123.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 8364)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8364)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8364
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123_length_8415_cov_18.165909"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1417 a         2738 c         2706 g         1503 t
ORIGIN      
        1 ccgggtcgcg cccgtgcacg ggcgcatcgc tgtcaaacga ccagccaccg aactgggtgc
       61 gctggtagtc ggccatggtc tgcgcaattt cggcctgggt gttcatcacg aaggggccgt
      121 actgcgccac cggctcgccg atcggcctgc cttgcagcag caaaaactcg gcgtcgttgc
      181 tgccgctgtt gaccagttcg acggccctat cggcacgcac ctcgatggcg gccgggctgc
      241 ggatgtcctg gccgcctatg ctgacggact cgcccttgaa gaagtacagg ttgcgccgcg
      301 tgccctggcc gccggcggct ggcagcgtcc agcgcgcgcc cggcgccatg cgtatcgtcc
      361 agatcgccac gtcagcatcg gcctgcgcag cccaagagtc gggcggcggt gccagtggcg
      421 tggcggcgtc ctgcaacctt ccggcaatca cggccacctg ggtagcgcgg ccttcggcgt
      481 ctttggtggt caggcgcgga atgtcctgcg accagaacat ggtgaagtgc gggtcgctca
      541 tcttggctgc cgccggcagg ttcagccaga tctggaacag ctccagcggg ttgtcgtttt
      601 tttcgtccag cagcgggaac atctcggcgt gcacgatacc cttgccggcc gtcagccatt
      661 gcacgtcgcc gcggccgaaa cgcgcggtgg cgcccagcga atccgaatgg tcgatcaggc
      721 ccttgcgcac gatggtgacg gtttcaaaac cgcgatgcgg gtgtggcgga aaacccggca
      781 ctttttcgcc gtggtacatg ctccagccgt ccttgcggct gaagtcctgg ccaatggcac
      841 ggcccgccag cgagaccgcc ggccccatgt cggcgttggc tttgggatag gcgtcgtcgt
      901 gataggcgca gaacaaaaag gggtcgatgg tttcccacgg aaaaccgaga ggctggacct
      961 gaacgatggg gctggacgta ggtgaaaaag aggacatggc gttgctttca ggagtctccc
     1021 tcaagcttac cggcatctgt tttccagtgc ttggcgccgc cacgcgagca cccacgcgag
     1081 ctggggattc cggcaacacc ggccaattcg ggacacgccg ttgtgggttc gtcctacagg
     1141 acactgccag caccgcaggt agattcacgg ttcgtcctgt tgcatctacc cagaaattat
     1201 tttaggcagt acaaggccga ggcaggaaaa actgcctgcg cacaatttcg cgcgtatttt
     1261 tcgctcaaat tccgaaagac ttccatgaaa tattccattc tgcttgccgc tctggtcgct
     1321 acccttggtc tggccgcttg tgaccgcacg gtggtcactc ctcctgccgt ggccgttcct
     1381 ggccctgccg gccctgctgg cgaagctggc tccaccggcg ctacgggttc taccggctcc
     1441 accggttcga cgggttacac cggcgcaaca ggttctaccg gctccaccgg cgcgacgggc
     1501 tccaccggcc ctagcggcgg tgctaccggc gcaacgggtg ccactggcgc taccggctcc
     1561 accggggcaa cgggcgcaac cggcgctacc ggcgacaccg tgattgttgt tccgcaaaag
     1621 taagcttcca gcaacgcgcg gccacccttt ccggggctgg ctggcgtgat gtgtgtgcgc
     1681 cacgagtgca cgtacatgaa aagaaaaggc cccagccacc gaggtgactg gggccttttt
     1741 catggccggg ctcaagcctt catcaggctt tcaatttcca gccggtgcga aagatccacc
     1801 acacggcgcc caggcacagc gccagaaagc ccaggatggc ggcgacgctg atgccgacgt
     1861 tgacgtccgc cacgccgtaa aaactccagc ggaagccgct gatcaggtag accacggggt
     1921 tgaacagggc aattttctgc cacagcggcg gcagcatgtt gatggagtaa aacgcgccgc
     1981 ccagaaaggt cagcggcgtc accaccatca gcggcacgat ctgcagcttc tggaagttgt
     2041 ctgcccagag accgatgatg aagccgaaca ggctgaaggt cagcgccgtc agaagcagga
     2101 aacagagcat ccagaacgga tgcgcaatgc tgtagggaac gaagatgcgc gccgtcgcca
     2161 ggatcagcag gcccagcagc acggacttgc tggccgccgc gcccacgtaa cccagcacca
     2221 cttccatggc cgacaccggc gccgacagca gctcgtaaat cgtgcctgac catttgggca
     2281 tgtagatgcc gaaggcggaa ttggagatgc tttcattgag taacgacagc atgatcaggc
     2341 cggggatgat gaaggcgccg taactgatgc cgtcgatgtc gcccatgcgc gaaccaatcg
     2401 ccgcgccgaa caccacgaag tacagcgagg tcgagatcac cggcgaggcg atggactggg
     2461 tcagtgtgcg gaaagtgcgc gccatttcaa agcggtagat cgcgcggatg gcgtgccagt
     2521 tcatgcagcc tccttctggg tggcgggctt gttggcatgg accaggctca cgaagatgtc
     2581 ttccagcgaa ctctcgctcg aatgcaggtc cttgaagtcg atgcccagtt cggccagccg
     2641 tttgagcagc tcgccgatgc cggtttcctc ctgctgcgtg tcgaaggtgt agaccagcgt
     2701 gtggccgccc tcggtcagct ccactggcag cccggccagc ccttcgggca cggcctgcag
     2761 cggcgcgcgc aattgcagcg tgagctgctt cttgccgagc ttgcgcatca gcacgttctt
     2821 gtcttccacc acgatgagct ggcccttgct gatcacgccg atgcggtcgg ccatctcttc
     2881 ggcttcctcg atgtagtgcg tggtcaggat gatggtcaca ccgctgtcct gcagcttgcg
     2941 caccatctgc cacatgccct ggcgcagttc cacgtcgacg ccggcggtgg gctcgtcgag
     3001 aaacaggatc tgcggttcgt gcgacagcgc cttggcgatc agcacccggc gcttcatgcc
     3061 gcccgacagc gtcatgatct tgctgtcctt cttgtcccac agcgacaggt cgcgcagcac
     3121 tttttcgatg tgggccgggt tggccggctt gccgaacagg ccgcggctga agctcaccgt
     3181 ggaccagacg gtttcaaacg cgtcggtcga cagttcctgc ggcaccaggc cgatcttggc
     3241 gcgcgccgcc cggtagtcgc gcacgatgtc gtggccgtcg gccgtcacgc gacccgaact
     3301 gggcgtgacg atgccgcaga cgatgttgat cagcgtggtc ttgccggcgc cgttgggccc
     3361 gagcagcgcg aaaatttcgc cgcgccggat ggtgaggtcc accgacttca gggcttcaaa
     3421 gcccgaggcg tagaccttgc tgagctgctc gatggagatg atgtctggca aatcggaatt
     3481 ccggttgatg tgggcgctgg tgaagatgca gcggcccgcg tcactgtaac cggatcgcat
     3541 gaaggttgct gacgacgggg ttgttcaccg cgcgcgggtg attcccggca tcccggaccg
     3601 gttgtcaggt cccgggataa ctcagttccg ggtaccagtc ccggccgcgg ccttcgggcg
     3661 tgctgtccag gatggtccac agcggcatca ggtccggcgc gccgcgcggg tcctggccgg
     3721 gatcggccgt ggacacgccc atctcgccgc tccagaagtg ccgcaggctg ccgccctgcc
     3781 gggtgaagac gttgaaggcc ggcacatcag catcctgcgc actcacatag tcgcgggtgt
     3841 agtcgccgct cacatccgaa tagagtttca ggtggcgcca gccgcgttct ttcttgaagg
     3901 ccagcaggcg tgttatcggc gagcgcgcca ccaccaccag cgcgatgcgc tgcgccacat
     3961 cctcggcttc gccgtcccag gccgacagca gcgaggtgca catcgggcag ggccgcttgc
     4021 gctgcggccc gaacatatag ctgtagacgg ccagcgtctg cttgtcggcg aacaggtccg
     4081 aaaaacgcac cgggccgtct tcaccctcga agccgtagtc cttcggcaac acgccgcccg
     4141 gcggcaggtc gcgccgctgc tgcgccacgc gttcgatgtg gcgccgcagc tcgatctcct
     4201 cggccagcag cgcatcgcgc gcacgccggt agtcctcgct ttcattgggg aaacgcaccc
     4261 cgttgttcag ggccagctcc cgggccggct tcagggtcgg tgtgggggtc atgggcgctc
     4321 ctgtatttca gccgttgcgg gccttcaccc ggcggtaggt ggcgctcatg aagcgcttcc
     4381 agctgccgtc ggcctgaatc acctgcgagg ccagctcgta cacctcgggt ccctgcaggg
     4441 tgatgatgtc ctggtaacgc accagcgtgc cgtcgccgct gaacgacggt ccttccgtgt
     4501 ccagcgtcag cactttgccg gccgcgtcca gcgagcccgc gtagtgccac aggtgcgtca
     4561 tcatcgaacc gacgaaggtt ccgacaaaag cgcttttctg cgggtcatag ccgagcgtga
     4621 tcagcatgtg ggccgggctg ccgtcgggca tgctgccctg gctttcgcac agcacccaca
     4681 actcgcccag gctgcgcacg gtttgttggc ccgcgtgttt catggcgggc tggcccgggc
     4741 ccatgtccgc ctggccttca aacgaccatt gacccaccag ctgctgcagc cagcggtgct
     4801 gggccagcgc tggcacggcg gcgcacgggt cttgttcggt attggacatg acagcactcc
     4861 tttcagggca tgggcagcgg gttaatgacc atccagccca ggccgaagcg gtcggtcacc
     4921 atgccgaagg acgaggcgta aaaagtcttg cccagcggca tctgcacctg cccgccgtcg
     4981 ctcagcgcct tgaacaggcg cgcgacttcc gcatccgtgg gggcggtgat cgtcagcgaa
     5041 aaacccttga actccggctt gccgccgaaa gccatgccgt ccgacgccat cacctgcgtt
     5101 tcgccgatgc gcaggttggc gtgcatgatc ttgtcctccg agcccggggg gcacatgtcc
     5161 ggcgactggg gctcggggtt ctccttgaag cgcatcatcg ccgtcacttc ggcgcccagc
     5221 gcggttttgt aaaacgtcag ggcctcttcg cagcggccgt caaaaaacag atagggctgg
     5281 acttgcatgt cggtctcctt gtcagggggt gggtgggtgg gggacgggga cgggggcggg
     5341 ttggcggaag tggcgatgca ggccggcata cagccgcaag gtctccgcca ccatgggtcc
     5401 atgcggcctc gtagacctgc tgcagcttgt tgccgtcgag cttgatcatc tgcatcatgg
     5461 cctgcatcac gcggttgacc ttggccggat ccttgtcggc cagcaccttg cgcatcacgc
     5521 tgggcacgat ctgccaggac acgccgaact tgtcggtcag ccagccacag gcctgcacgc
     5581 tgccgccctc ggacagcctg tcccagagtt catcaacctc ttcctgggtc gggcaatcga
     5641 caaacagcga catgcccggc gcgaagccga agtccgtgcc cgcattgagc acgaagaagg
     5701 tctggccggc aatttcgaaa ctgccggtca tgaagctgcc tttggggacc ggggcgccat
     5761 cgccgtaacg ctggatgtcg ccggccttgg cgttcctgaa caccgaggta tagaacttga
     5821 gcgcatcttc agcttggtct ttgaaggtca ggaaggtggt gattttttgc atgggaatgc
     5881 tccttggagt cgctggccaa ccattgcctg cgttgtcaaa cagggcaaac aggggtattg
     5941 tgaaggtgtt gtgtacaccg tccacaaaag aatagcaaac ataatggacg ctgtccacat
     6001 aaaacgtaac aaaccatgac agcgctgtcc atcacgccgc cgatccctgc gccggcccgc
     6061 agcacctacc gccacggcga cctgcgccgc gcgctgctgg aagccgggat cgagctggcg
     6121 cgcgcgggtg ggcccgaggc cgtggtgctg cgcgaggcca cgcgccgcgc gggggtggcg
     6181 cccaacgccg cctaccggca tttcgccagc cgccaggccc tgttgcaggc ggtgcgtgcg
     6241 gccgcgctgt cggccgtggc ccgggccatg gaagccgaac tggccgccgt gcccgccggt
     6301 ttgccgccag ccgacttcgc ccgcgccagc gtgcgtgccg tcggcaccgg ctacctgcgt
     6361 tttgcccagg ccgagaccgg gctgttccgc accgcctttg ccgcgtccga cgacatgaag
     6421 gatgaagcac cgcgcgaccc cgacagggcc ggcccgggcg gcctgaaccc gttcgagctg
     6481 ctgggcgccg cgctcgacaa gctggtgcag gccggtgtga tgccgcccga gcgccgcccc
     6541 ggcgccgagt tcctggcctg gtccgccgtg cacggcctgg ccatgctggt catcgacggg
     6601 ccgctgggcc cggtgatcgg cccgcagatg cagctggtcg gccagcgcct gctcgatatg
     6661 gtcgaaaaag ggctgtgaag acctcacccc ccatgcccca gcacatcgga atcgtcggct
     6721 gctccgcgga aggtgccgcc ctttgttacc gcaccatttg cgcggaaggt gccgcgctgc
     6781 tcggcccgca cgcgcacccc gaggtttccc tgcacacgcc ctcgctcgcc gcctatgtgg
     6841 actgcctgga ctgcggcgac ctgcagggcg tggccgacct catgctggcc tcggcgcgca
     6901 agctggcggc ggccggagcc gacttcctga tctgccccga caacaccatc caccaggcca
     6961 tgtcgctggt ggtgccgcac tcgccgctgc cctggctgca cattgccgag gtggtggcgg
     7021 acgcggccgt gcagcgcggc tttcgccgtg tgggcatcac cggcacgcgc tggttggtgg
     7081 acagcgcggt ctacccggac aagctggtcg cgcgcggcct gcagtacctg cggccaaacg
     7141 atgccgagcg cgacgaactg ggccgcatca tcatggacga gctggtgtgc ggcatcttcc
     7201 ggccagaagc cgtggccagt ttccagcgcg tgatggaacg aatgaaaggc gaaggctgcg
     7261 atgcggtggt cctcggctgc accgagattc cgctgatcat caacgacggc aactcgccgc
     7321 tgcccacgct ggactcgacg cgcctgctgg cgcgggcagc cttgcagcgc gcggtgcacg
     7381 gcagaactta ggcatcaaat cggcctgtag cccaatcagt acctgcgcaa gctgctatca
     7441 aaagagaagt gtcggacgct gtggcccagg cacagcaccc tcatcggcag cgcgccggct
     7501 cacagcattt tcttgggcac cagggcaatc gtcagccctt tggccgtgac ggtgatgctg
     7561 cccggttcca gccccaggcc gtcggtcagc atcaggtctt gcggcttgag tttgtgcagc
     7621 accacttcct tgagcgcctg gctggccagc gtgggagcgt acgcctgcag cagcgccgcc
     7681 gggccgggcg ccatgtcgtc caggcgcagg gcgttgagcc gaatctggtg cgcgcgcagc
     7741 gtctggtcgc tggcttcata acgcagcgca aagtccacgt cgaaggtgcc ggtcgaggtg
     7801 cgtgccagcg ccgggccggc cgcgtccacc accatctcgg tgcccaggcg gttcagttcg
     7861 ggcagcaggc gcacgcgcgg cgcctgcaca ttcaggttca gcaggctgcc catctcataa
     7921 cgacgcggga agcgctgtgc cacggcctgc tgcagctgtt ccagcgtcac cttgtagccc
     7981 gttccgccgg gcgcgtccgg tgtggcggcg cgcgccgcac ccgccgcagc ggcaaagacg
     8041 atccagccgg gcggggtcaa caacagtctg cgtttcatcg ggccttcatg aaaaagcgca
     8101 tgcaggcatg caatcggtca ttgtgcgccg cgactgttaa ccgccctgtt gatgcggttg
     8161 tcgggcacat cccgttacgt gcggacatgc gccgcctaca agcccacaca cacacgacac
     8221 ctgcgaagcg ctcaatgcat caacccggct gctagggcct gttcacactg tttatgcaag
     8281 atgcgttgcg agtcaaaaag gtcatgcgtg aggcgcaaaa cgcagccggg ctggcgcccg
     8341 gcaaggcttt gcaacgctgc gcat
//
LOCUS       DAIDFG010000124         8366 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_124_length_8366_cov_18.429912, whole genome shotgun sequence.
ACCESSION   DAIDFG010000124 DAIDFG010000000
VERSION     DAIDFG010000124.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 8366)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8366)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8366
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124_length_8366_cov_18.429912"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1514 a         2695 c         2681 g         1476 t
ORIGIN      
        1 gttgtccgag cgcagcgagt tctgcggacc cccgccgcaa tcgagcagcg caggttgcct
       61 ggagcgaagc ggaaggaccc agacagtcgg gtcgcctttt ctttggtgac tttcttttgg
      121 cgacacaaaa gaaagttact tgccgccggg caacccccgg cttgcaggca aactgcagcg
      181 cttcaaaaaa atgagtagtg agcaaacaca tggattccgg atcacgtccg ggatgacagc
      241 ctgaaagatg ggttgctata aacttaatag ctgttcgcgc ccaccgggta tgggctacaa
      301 cccgatttaa tgccaaatcc ggcaagatca gacagtcccc cgagcgaagg actcagtcgc
      361 gcaccaccag aatcggcaag tgcgcatgcg ccagcacctg cgctgcgacg ctgccgagca
      421 cgagcttttc cagtccgcgg cgcccgtgcg aacccagcac gatcaggtcg gcaccgacag
      481 caccggcggt ctccagaatg ccgcggtaaa tcgcgtggcc ttcgatgatg gaggccttga
      541 ccgtcacacc tgcggcttcc agcacctgcg tggcgccctg gagggcttcg ttggcttccg
      601 ccgtggccgc cccgaggtac tgggcctggc cgtaggcgaa atcggtgccg accccgctga
      661 aggcgtaggg atcgatcacg tagatcaccg tcaccgtgct tttgaaagcc tgggccagcc
      721 cgcaggcctt ctcgaccgcc atcatcgagg tggacgagcc gtcgaccggc agcaggatgt
      781 gtttgaacat gatcaggctt ccttcagaaa aacgcactcg ggccgggaca tcagggttcg
      841 atcttcacac ccttccagaa agccacatgg cccttgatgt tgtcggcctc gggtttcggg
      901 tcggggtaat accaggcggc gtcggtgttc atctcgccat tgaccagcag cgagtagtag
      961 ctggcctgcc ctttccacgc acacatggtg tggtggttgc tgaaggtgat gtagtcgcgg
     1021 ttgagcgaac tttcggggaa atagtggttg ccttcgagca ccacggtatc gtcgctttgc
     1081 gcaatcactg cgccgttcca gatagccttc atgtcgcttc tttcatgggt tgccggggtc
     1141 cgccgtgtgc ggagggggaa aaggataaaa aggggaaagg gggttgcctg ccaacaatct
     1201 tcgcacactc tgcagggaat gcgaggccgg ccgggaaaag gaaaaagaca aaaagaaaag
     1261 cgtgatggtc acccatcacg ctttgacgca gttctgtgca ggatcacgcc gccgcagcgg
     1321 cgacccatca ggccatgcgc aggttgttgc cttcaacaat cacgcgggag ccgacgggaa
     1381 tcgagctgga cacttccacg gtgcggacag aaccgtcgtt catgcgcaca cgcacctggt
     1441 agacggtgac cttcttcatg tttttctcga tctggttgcc ggcaaacgca ccgccggcga
     1501 cgcccagcac cgtcatggcc gtcttgccgc tgccgccgcc cacctggttg cccaacaggc
     1561 cgccaaccac gccgcccgca acggtgccca ggccgacggt ggtgttgccc acggcgacac
     1621 cgttgacctc gccctggcgc tgcacggccg tcacggcttc caccgtgccg cagtcggcgc
     1681 aaaccacggg cttgggtgcc ggtgcctggg ccacgaccgc aggccgcggc tggacgaccg
     1741 gtttggccgg cgccggcctg gcagtggtcg tactggtcgt accgttactg gccagcttgg
     1801 ccggggtgct ggtcgttcca ttgctggcca gcttggcagc cgtgctggac tgtgttgtgg
     1861 gggcttgggc ctcggtgccg cccggctgcg catccgagcg attgaccacc agcgcggcgg
     1921 cgagcgccat caccgtgacc ccgagcacgg ccacaatcgc gatcagcgat ttgctgtgct
     1981 gcatgcgttg cgaaaaggcg ggctggctgc cgggggtggc tggtgtctgg ttatttgtat
     2041 ccatgatggg tactccttgg ttgagcctca gttgtaacca actcatgtga acgcggatgt
     2101 aggacgacgt gacattcgta agggaaggtt agcgcgaccc atctcaaagc gtgcgttatt
     2161 gcgcacttgc gaacagcttc acccgcggct caccgcggct tgacctgcgc tttacacgcc
     2221 gccgtgcggg tgggtcggat caatccacaa cacctgctcg ggcttttcgg ccggcgcaat
     2281 atcgaggttg atggccaccg cctcgccgtc gctgcggcaa agcacacact cgagcagttc
     2341 ggtcggactc gcgttgattt cctggtgcgg cacatagggc ggcacataga tgaagtcgcc
     2401 cgggcccgcc tcggcggtga actccagatg ctcgccccag cgcatgcggg cacgacccct
     2461 gacgacataa atcacgcttt ccagatggcc gtggtggtgc gcaccagtct tggcgttggc
     2521 atggatggtg acggtgccgg cccagagttt ttgcgcaccg acgcgggcga aattgatggc
     2581 cgccttgcgg tccatgccgg gcgtggacgg cacattgccg tccagctggc ctccggggat
     2641 cacgcgcaca ccgtcgtgtt tccagtcggt gccttcgggc gcgtggctgt gcccgtgagg
     2701 atggctgtgg ccggtgtcgt ggctcatggt gtgtctccga gaaaaaaacc agtttctagt
     2761 ccagcagggc agcgtacacc aggctgcgaa acagcggccg gtagtggtcg cgctggttgg
     2821 gcgcctggat catcagcacg gcatacaggt cttcggccgg gtccacaaaa aacgtggtgc
     2881 cggcgatacc gccccagtag tacagcccct tcgagccggg cacggacgcg atgccggtgg
     2941 cggtgcgcac ggcaaacccg agaccgaagc catagccggg cggcaacacg tcacccgtga
     3001 cggggatggc gcccagatgg tcggcggtca tgaagtccac ggtacgcgaa cccagcaggc
     3061 gcacgccatc gagttcgccc cggttgcgca tgaactgcag aaaacgcgcg taatccatgg
     3121 ccgtggacaa caggccaccg ccgccgcttt ccatggccgc cgcacggcgc ggctccagca
     3181 cgcgcatcgg cacgccgccg tccgggtcgt gggcaaaggg ctcggcaata cgcgggtgat
     3241 cggtctcggc caccgagaaa ccggtgtcgt gcatgcccag gggaatgaag atgtggcggc
     3301 gcaggtactc gcccagggtc tggccgctga gcacttcgac cagccgcccc agcacgtccg
     3361 tcgcgcggct gtattcccag acgctgccgg gctggtacat caatggcaaa ccggccagca
     3421 ccctggaaaa ctccgcgttg ctgcgctcgc gcgagcccat ctgcagcctg gcgtactggc
     3481 gctgcacgtc ggcgtttccc agaaattcat aggtcaggcc ggccgtgtgc cgcagcaggt
     3541 cctgcacggt ggcgggccgg tggacatcgt ccagcgtcac ctggccgttt tgttccaccg
     3601 cgaccttctg gttggcaaat tccggcaggt gcctggccac cgggtcactg agcagcaacc
     3661 tgccctgctc caccagcatc atcaccgcga ccgacaccac gggtttggtc atggagtaga
     3721 tgcggaaaat cgaatcacgg gtcatcgggc tgcccgcagc cgggttctgc cggcccaggc
     3781 tttcgaacag ggccaccttg ccgcggcgcg ccaccagcac caccgcgccg ggcagtcgcc
     3841 cgcggtcaat gtcggcctgc agcacggcca gcaggcgctg caagcggtca gggcacaggc
     3901 ccacctcggc gggcgcggca aaagccagtt cggtcatgct gcccgcctca ggcgccgtaa
     3961 cgctcgcgcg ccagcgccgc accctgggcc aaggcctgca gcttcttcag ggccacgtcg
     4021 cggtccatgg gcgccaggcc gcagttggtg cagggcagca ggcggttttt cggcacgtac
     4081 ctgagggctt cgccgatggt gtcggcaatc tgctcggggg tttcaatctc gtcgctcgcc
     4141 acgtcgatca caccgaccat cacgtccttg ccttcgagca gggccatcag gtgcggcggt
     4201 acatgcgagt ggtaacactc gaggctgacc tgctggatgc tgcttttcgc cagcgcggga
     4261 aggatttgct catactggcg ccattcttcg cccagtgttt tcttccagtc gtcgttggcc
     4321 ttgatgccgt agccgtagca gatgtgcacc gccgtcgtgc aggtcaggcc ctgcgcggca
     4381 cgctcgagcg ccttcacgcc ccactcggcg gcgtctttca tgtagacgtt gaaggccggc
     4441 tcatcgaact ggatgatgtc cacgccgtcg gcctgcagcg ccagcgcttc ctggttcagc
     4501 agctcggcaa aggccatggc catcttcacg cggtcgccat aaaactggtc ggccacggta
     4561 tcgacgatcg tcatcggtcc gggcagggtg aacttgatct ttttcgtcgt gtgggcgcgc
     4621 agcagctgcg cctcggccgc atgcacacgc cccttgagct tgagcgcgga cacgacctgc
     4681 ggcaccatgg cgtcgtagcg gttgttgcgt atgcccatct tgaccttgtg ctcgaagtcg
     4741 atgccttcga cctgctcgag aaagccgtgc acaaaatgct ggcgcgactg ctcgccatca
     4801 ccaatgacgt ccaggccggc atcctcctgc gccttgatcc agagcagcgt ggcatcggcc
     4861 ttggcctggg ccagttcggc gccttcggcc ttccactgcg gccagagttt gtgggtttcc
     4921 gacagccagg caggtttggg caggctgccg gcaatggtgg cgctgaacag gggtgaagtc
     4981 atggtggggt cctcgggttg ttgtctgatg gggttgttgt ctgatgaagt gatgggttca
     5041 ggcctggccg gccggaggtg gggtgctgcc aaacgcctgc gccagcaact cataggaacg
     5101 gcggcgcgcc accgggtcat gcgtccaggt gatgaccacc agctcctcta cctgcaggct
     5161 gtcggacagc gcacgcagct tgccggccac ctgcccggca ctgccgacca gcgccgtgcg
     5221 ccgcagccgt tcggtctcgg cctgctcggc cgcgctgtag ggccgctgcg cgacttccgt
     5281 cggtgactgc agcggcccca gcgcgccctg ctggcggtcg attttccagc gctcgcggct
     5341 ggtgaaaagc tgccaggctt cggcttcggt ctcagcggcc agtgcccaca cacacagtgc
     5401 ggcctgcggc tcgggatgtt gcgggctggg ccggtacagc tcgcggtaaa tggccagcgc
     5461 ctcttcggcc ccctggccgt cggtgatgaa atacgcaaac gcataaggca ggccgtaatg
     5521 cgccgccagt tgcgcgccgt agttcgagct gcccagcatc cacagcgtgg gcgcgcccgg
     5581 cccggtcggg ttggcagtca cgccgcggcc cgggtggcct tcgggcagat caacgccgct
     5641 gacccaggcc tgcagctcgc gcacctggcg gggaaaatca tcggccgcat ggcgcgcatt
     5701 cgggttgagc agctgggcgg tgcgcccgtc cgaacccggc gcacgaccga caccaaggtc
     5761 gatgcggccc ggggccagcg cctccagcac gcggaattgc tcggcgacct tgagcgccga
     5821 ataatgcggc agcatcacgc cggcactgcc gatgcgaatg cgctgcgtgg tcgcggcgat
     5881 ggcggccatc agcacctcgg gcgcgctgcc gacaatcgtc gggtggctgt ggtgctcgct
     5941 gacccagaaa cgctcgtaac cgaggccctc gcagtgctga gccagcgcca gcgtttcgcg
     6001 gatggaggcg tcttcgctgc ggccggtgac ggcaacggac tggtcgagaa cggacaatcg
     6061 gatcatgcat gaactatagc cagcctgagg tgcccggaca cgcgggcgga caaaggtctg
     6121 ttccaatagg gggttaccac ccgcccctca cccaaccagc agacccccat ggcactcaaa
     6181 gccaccatct acaaagcaca actggccatt gccgacatgg accgcggcgt ctacgccgac
     6241 cacaacgtca cgatcgcgcg ccacccttcc gaggccgatg aacgcatgat gatccgcctg
     6301 ctggcctatg ccctgaacgt gacggccgac gacaacaaag gcaagctgga gttcgccaag
     6361 gacctgtggg atgtcgacga ggcggccctg tggcacaagg actacaccga ccagatcctg
     6421 cactggatcg acgtcggcca gcccgacgac aagcgcctga tgcgcgccgc cggacgcgcc
     6481 gaacgcgtca ccgtctacag ctttgccagc agcacgccgg tttggtggaa gaccatcgaa
     6541 agcaagctca cgcgcgcggc caacctggtg gtctggcaga tcgaggcggc gcaaagccag
     6601 gcgctggcca ggctggccaa ccgcaccatc cagctgcagg tgacgattca ggacggcacg
     6661 gtctggatga gcaccggcag cgagtcggtc gagatcacgc cgatccggct cacggctggc
     6721 ggctagaact cacgccgtca ggatgcctcg ccggcctgct gctggtaagg cggcagccgg
     6781 gcaccgcagt tgaagcagaa attcgcatcg gcggcgtggc cttcggtcag gcattcgtgg
     6841 caggtgcgcg tggtcggcgc ctggccagaa tgcatgcggc gctgcgctgc catctccgcc
     6901 gtcacgatgc cggtcggcac ggccagaatg ccccagccca tcagcatcat gaaagaagcg
     6961 atcaaccggc ccagatcggt cttgggcgcg atgtcgccga aacccacggt agtgacggtg
     7021 ctgatggccc agtaaaccga ggtcggaatg ctggtaaatc cgttggtctg gccctcgacc
     7081 acatacatca gcgtgcccag gaccagtacc accatcagca cggccgacag aaacaccagg
     7141 atcttgcgcc cgctggcggc gagggcactc accaggaact ggtattccgc gacataggcc
     7201 gcgagcttga acacccggaa tatacgcagc aaccgcagca cacgcacatc gatgagggca
     7261 tgcagctcgg ggacaaacag cgccaggtag gtcggcaata cagccagcag atcgatgatg
     7321 ccgaaaaagc tggtcgcgta cttcagcggc cggcgcacac acagcaggcg cgcgatgtac
     7381 tcgacggtga acagcagcgt gaaaacccac tccgcggagg tcagtgcgtg gccataaacc
     7441 aggcgcacgg cctgcacgct gtcagccatc accacggcaa tgctgagcag gatgacggcg
     7501 atcagggcct ggtcgaacag gcgtccggcg cgagtgtccg cttcaaaaat gatggtgtac
     7561 aggcgcagcc gccagccggc cagcggcttg tcgtgcaagg gatcagagat gggcggtgaa
     7621 ggtttttcca tgagcgcaag caacgcctct ggagcgtcgc acctgttcga agtccgggag
     7681 acgaacctta accgatgttg ccgggcatgg gctccggctt ttccagattg ctcttgtttt
     7741 gatagctatc tgcgcccgcc cgacaagggc tacaggcact tttcatgcac aaacttcagg
     7801 cggtggcgac ttccgcctcc tgcagggtcc gccacatcac cttgcccgag ccgcttttcg
     7861 gcagggcatc gacaagctgc acaacacgcg ggtacttgta ggcggccatg ttctccttgc
     7921 accagtcgat gatgtccgcc tcgctgacct gccccttgtg gctttgccgc agcaccacca
     7981 ccgccttgac ggtctcgccg cggtaggcgt cgcgcgtgga gatgatgcag gcctcctgga
     8041 tggccgggtg cttgaacatc agcgcctcca cctcggccgg ccagaccttg aagcccgacg
     8101 cgttgatcat gcgtttgagc cggtcggtga tgaagaagta gccctcttcg tcaacgcgcc
     8161 cgaggtcgcc cgaacggaag aaagatttgc cgtcgatctc gatgaacgcg gcggcggtgg
     8221 cctcgggccg cttccagtag cctgagaaat tctgtggccc gcacatgatg atttcaccct
     8281 gctcgcccag cggcatttcc cccaggctca aggggtcgat cacgcgcgca tccacattga
     8341 gaaaaggcac acccaggcat tgctgc
//
LOCUS       DAIDFG010000125         8362 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_125_length_8362_cov_20.404117, whole genome shotgun sequence.
ACCESSION   DAIDFG010000125 DAIDFG010000000
VERSION     DAIDFG010000125.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 8362)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8362)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8362
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125_length_8362_cov_20.404117"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1583 a         2688 c         2544 g         1547 t
ORIGIN      
        1 tttcatttgg cgggccgcga gcgcgaactg ctgattcgcc cgatagggcg cacggtgtac
       61 ctcatgccac cctacctgct ggacgatagc ctgtcgaact ggctggccgg gcgcctgctg
      121 gccacgctgg acgatgtctt gagccaaact ctgaatgctt gcgatgctga tagaccacct
      181 gaaccgccaa cggcttgagc gcgaagccca gggcctgcag cgcctgcgcc gcatcgccga
      241 gtcgccgtgc gcgccaaggc aatgggtcag ccagggcggc cagccggcgc gcgagctgct
      301 ggtctttggc agcaacgact atcttggctt agccaatcac ccggccctgg tccaggcgct
      361 ggccgagggc gcgcagcggt tcggcgtggg cagcggcgcc tcgcacctga tcagcggcca
      421 ctcgcgcgcc catgccggac tcgaagacga cctggctgcc tggctggaac cctgcattcc
      481 gaatgccgcg gcgctgtatt tctgcaccgg tttcctggcc aatgtggcgc tgcttaccgc
      541 cctgggcgat gccagcgcca ccatctttgc cgacaagctc aaccatgcct cgctgatcga
      601 cggcgccctg ctggccaggg cgaccatgca gcgctacgcc cacaagaatc tcaccgtgct
      661 ggagcgccag ctggaacgct gcacgacacc gatcaagctg atcgtgaccg atgcggtctt
      721 cagcatggac ggcgatctgg ctgatctgcc ggcgctcatg gcgctggccg agcgctttga
      781 cgcctggctc atcgtggacg atgcccatgg ctttggtgtg ctcggcgccc agggccgggg
      841 cagcctgtca catttgggcc tgcgcagcga acgcctgatc tacatgggta cgctgggcaa
      901 ggcagccggt gtaggcggcg cctttgtcgc cgcgcatccg accatcatcg actggctggt
      961 acagtccgcc cgtccttata tctacaccac cgccgcgcca cccgccgtgg cccatgcgtt
     1021 gcgcgagagc ctacgcatca tcagcggcgc cgagggtgat cagcggcgtg cgcaattgct
     1081 gcaacggatt actcagctac gtacacagct cgccgccctc atcgtcgcac acccatcgct
     1141 gggctggcga ctggccgact ccagtaccgc gatccagccg ttgatagtcg gcgacaatcc
     1201 cgccgcgctg gcactgatgg cggccttgga tgcgaaaggt ctgtgggtgc cggccatacg
     1261 cccgcccacg gtgccggtgg gcacggcgcg gctgcgcatt acactcagcg ccagccacag
     1321 cgcccgggac gtgcagcgtc tggtcgatgg cctggcctgc gccgcccggg agttgtcatg
     1381 accgcacccg gcatgagccc tcaagcgctg cacggctgct ttgtcaccgg caccgacacc
     1441 ggcgtaggca agacccatgt cagcgccgcg ctgctgcact ggtgcgcgca gcaaggttgg
     1501 cgcagcgcgg gcttgaagcc cgtggcggcc ggcaccacgc tcatcgacgg gcagcgcgtc
     1561 aatgaggatg tgcaggcctt gcgccaggcc agctcggtgc gactcaccga gacgcaagtc
     1621 gggccgctgc agttcgaggc cgcttgcgcg ccgcagattg ctgccaccct ggagcgccgg
     1681 ttgatcgacc cggcgacgat tctgtccgcc gcccgccatc tggctagatg cgccgatgtc
     1741 gtcgtagttg aaggcgtggg cggcttttgc gtgccgctcg gccccgaatg ggacacggca
     1801 gacctggcgg tagtcatagg ccttccagtg attctggtgg tcggcctgcg cctgggctgc
     1861 atcagccatg cgctgctgac ggccgaggcg attcggacgc gtggcttgcc gctggccggc
     1921 tggattgcca ataccgttga tcccgacatg gcgcacagcg aaggcaacct ggccagcctg
     1981 caccatgaaa taacgcgccg ccaccagacg ccctgcctgg gcgtggtgcc ctggctggac
     2041 acaccaacac ccgccgccgt ggcggcccac ctggacgctg tggccctcaa aagggtgttc
     2101 actgcatccc ttttgtagcg acccctttaa cgacctttcg tacccacctt cttcacctga
     2161 gaaaactgca tgactaccat taccaccatt cctgtttcgt ccctgcgccg cgcgcccgca
     2221 ccttccaacg caccgaaggc cccgctccgc tgggaagtcg ctgacgtggc ggccctgtac
     2281 gagctgccgt tcatggactt gctgtttcgc gcccagcagg tgcatcgcga aaattttgat
     2341 gccaacgagg tgcagctctc gacgctgctg tccatcaaga ccggcggctg cgccgaagac
     2401 tgcggctact gcccgcaagc ggccagcgcc gacagcggcg tggatgccac caaactgatg
     2461 ccgctgaccg aagtgatgga ggccgcccag gccgccaaag accagggcgc cacccgtttt
     2521 tgcatgggcg ccgcctggcg cagcccgaaa gagcgtgaca tggagaacgt gaccgagatg
     2581 gtgcgtggcg tgcgctcgct aggcctggaa acctgcatga cgcttggcat gctgcaggcc
     2641 gagcaggctc aggctttgaa ggaagcgggt ctggattatt acaaccataa cctggacagc
     2701 tcgcccgagt tctatggcga gatcatcact acccgcacct atcaggaccg gctggacacc
     2761 ctgggctatg tgcgcgaagc cggcatcaac gtctgctgcg gcggcattgt cggcatgggt
     2821 gaaagccggc tccagcgcgc aggactcatc gctgagctgg ccaacctcag cccttatccc
     2881 gaatcagttc ccatcaacaa cctggtaccc gtgcccggca cgccgctggc cgatgccccg
     2941 ccgctggacc cgttcgagtt cgtgcgcacc atcgccgtgg cgcgcatcac catgccgctg
     3001 accatggtcc gactgtccgc agggcgcgaa cagatggatg aagccttgca ggtgctgtgt
     3061 tttgctgcag gcgcgaactc gattttctat ggcgacaagc tgctgaccac cagcaacccg
     3121 caagctgacc gtgatcgcca gctgtttgag cgcctgggct tgaaagtcca gggcgcgcgc
     3181 cctgcagcgc ctacagcgca gtgaccatga gcgcagcaaa taggggcgat aggggcgcgg
     3241 agccgagacc gctcgcgctg gctcggcccc tcgtggatgc cgtgttcctg gccaatccct
     3301 acccgaccta ccgggccttg cgggaggccg gaccaattca ctggagcgag gagtttttcg
     3361 gcggcgcctg gctgctcacg cgccatgccg atgtcgaact ggtgctgcgt gatccgcgct
     3421 tttccgcgca gcgcaccggc acctgggtca agggccgcga gggcgccccg ggcgagctga
     3481 gcggctttca gcagctgttt gcccgcgcgc tgctgtttct cgacgcgccc gaccatccgc
     3541 gcatcaggaa ggtgctcaat gccggctttc ggcccgacat cctccaaggg ctggcgccgc
     3601 acattgagca ggtggtcacc gagctgctcg accgggtaga tcaggtagag cacctggaca
     3661 cgtttgactt catcgaggcg gtggcccgtc cgctgccggt gcgggtgatc acgctgctgc
     3721 tgggcatcga gcggccgcag gaagacttca tggcctggtc agaagacctg gccagcttca
     3781 ttggcgagcc taggccttcg cgcgatcagg ccaggcgtgc ccaggccagc ctggtggcca
     3841 tgagccgtta ctttgaatcc ctgctggcgc tcaagcgcca gacgccgggc gacgacctgg
     3901 tcagccggct ggtcgccgcc gaggccaggg gcgagatcca cgatggcgcc gagctgctcg
     3961 cgcaatgcgc aatgctgctg tttgccggcc acgagaccac acgccacctg ctgggcagcg
     4021 gcgtgcaggc gctgctcgcg catccggcgc agtggcagcg ccttcagcgc gaacccgagc
     4081 tgctgcccgg cgcggtgcgc gagctgctgc gctttgacag cccggtgcag tacaccgggc
     4141 ggcgcgtggc caccgatctg gtgctgcatg gccagcagct gcggcgcggc gagctggtgc
     4201 tgcccctgat tggcgcggcc agccgcgatc cggcccgcta ccgggatccc gacacgctgg
     4261 acatcacgcg cggccagggc gcttcgctgg ccttcggctc ggggccgcac gtgtgcatgg
     4321 gcgcggcgct cacgctgatg gaggcccaaa tggtcttttg gcagttgctc aaaagatggc
     4381 ccgggctcag cctggccgat gccgcgccgc gctggggcga caagccagct tatcgcgggc
     4441 tggtggcgct gccgctgcgc tctcgggcaa gtgcttgaaa gtggcccata tggcaggttt
     4501 acagcatggg ggtgcggcgt aatacttgga ctggtttatg cggtgattcc aaggctcgcc
     4561 cggtattcaa gaggactgag tgagccaagg gatattttga tgcgcttttc gttgtaccag
     4621 cggatgcatg agtcaactat ctggatgaat tggtccacag tgatgtcctg ccagcgtcga
     4681 ggatagaaca gttccgtttt caatcgtccg aagaagccct cgcatgcggc gttgtcgggt
     4741 gagcatcctt tgcgcgacat cgaacgaatc agctttgcgt tgtgcatcct tgagagccac
     4801 ccaggccagc gatagtgggc tccacgatca gagtgaacga caggccggtc tcctgcggtg
     4861 gctaccgtct cgatggcagc atccaacatc gtgttcacca ggtcagagtc tggacgggta
     4921 aacacctcca ctttggcaca ttgcgatgcc gtttaggtgg gagcgtccat cccattgctt
     4981 acggccagca cgtaacgctg ctcaatgagc gatcagaaca tggcaccaat gactgccaag
     5041 gcatacgcca cggatcgtag tgccaaacct cccacaaatg gttagccctc tgaatcactg
     5101 acgttcaagg ggccttttct ggttttgtcg cattagccag tcgaggaaaa aatttgggcg
     5161 aattcttcaa gcgcgcgctg gccccgggcg cacttatgct gccagtgcat aaaactgctt
     5221 ggcgaacgaa agtcccattc ctttttcggt gctcatttgg ccttttcgga tcatgtgcaa
     5281 cagctcaata cccgccagga ctgcgctggc agatctgaat gacttgaagc cgttttggcg
     5341 catcgctttg tcgaaaaacc gggtcgccgc agcggtatca cgcctggcac gcaacaggaa
     5401 atcgatggtg gcgccttcct tgtccaccgc acgatacagg tacttccact gaccattaac
     5461 cttgatgtag gtctcgtcca tacgccaact ggcgccgact gcccgcttgt gctttctgaa
     5521 taccttctcc agcaacggca aaaacttgat cgcccagcgg ctgacactgg agtgatccac
     5581 gtacacacca cgttcctgca tcatttcttc caggttgcgg tagctcagtg ggtacgctgc
     5641 gtaccaacgg acgcacagca gaatgatttc cttcggaaat cgcttcccct tcatcttcaa
     5701 catcgcccgt cccaaccatt gaatttcagg ctgtcagcat aacgccatcc ttggctggga
     5761 ctgctaatgc gacaaaaccg gtcggctcgc cgtcataggc accatcggca gtgacctggc
     5821 caattttgtg ttcgatctga tcaagcagtg gacccacttg ggacggatca tcaacatcct
     5881 gttctgtcag tatcgaggct acgatcaacc cagtattggc atccaccgcc aggtgcagct
     5941 ttctccagct acggcgtgac ttctggccat gattttcttg caaccattcg cccgcaccaa
     6001 agaccttcag ccctgtgcta tcgatcaaga tgtgtgcagg ccctgcgggc agtgcgcacc
     6061 gcttggagat cgacttcaag cgcatggcgc gtcgactcac cgtgccgtga tcgggcgcct
     6121 tcaggtccag ctccagcaga tcaaagatcg atgccatcag gccttcggtt tgtcgcagcg
     6181 gttggcgaaa cgccaagcgc aacttcaggc tggttttgat cgccaaatcc gagtagaaac
     6241 cttgcccacc aggtgtgctg tgtggctggg ctgcccaatg atccatcgct tcagccgtga
     6301 cccacaaggt caggctaccg cgggcgcgaa gtgccgcgtc gtactcagcc cagttgcgca
     6361 ccgaaaactt catcttcgga atatggtggc ggcacttggc gttgtgtttg ttgggcatgc
     6421 ggttggtcag atgtcttgat cagggctgac gagcctatca ggccgccgcc gagcatccgt
     6481 gcaacaacgc ccacgccagt gttacccgaa aaccgggggt tttgaaccac gccaacaacg
     6541 cccatgtttt gtctctcttt tgattaatgg caacatggaa cgggaaaggc acgggggtct
     6601 ggcggacgcc gggagtccgc tcaaggtctc aggtaaatgg cagccagcac aaacgcggcc
     6661 atccacaggg tttcaaccag cagcagggtg aacggtttcc atccggcgcg ggccaattgc
     6721 cggaaagacg tcttgatacc cagggcagca atggcgacga ccaggcaaat gcgggacaca
     6781 ttgctcaggc cctcctgaac ggccacaggt actccaccca tcgagttgag agccaccagg
     6841 gccacaaaaa cccacagaaa ccagggcacc agcgcctgcc tggctgcgac cgtgccgtct
     6901 tcagactgct cacgcccctt cttgaacata ctggatacca cgaccacgac cacggccagc
     6961 atggacaccc ggaacagctt gacgatggtg gcgtagtcac ccgtttcatg gttcagcata
     7021 taacccgcgc cgacaacctg ggccacgtca tgaatggtgc cgccaaggaa taggccggcc
     7081 aactccggcg gcagatgcaa tactttggcg atcaggggat aggccaccat ggccagcgtg
     7141 gacagcacag tcaccgtcac caccaccatc agggtgaagc gctcgctttc cttgttgcgt
     7201 ggcagcaccg acgaaattgc cagcgccgcg gaagcgccgc aaatagcaac ggcgccgccc
     7261 gacagcacgc cctgcgcccg cgtcaggccc aggcgccgtc ccagcaggct gccgcacagc
     7321 attgtggtca ccacggcggc aaccacaatg gcggcggtcg accagcccag cccggcaatc
     7381 tgggacgcgg tgatgcgcgc acccagcagg ccgacgccca ggcgcagcac ggtgcgcgaa
     7441 caaaactcga tacctggtct ggtcttggcg tcatggctga ggtagtgaaa agccacgcca
     7501 aagaacagcg catacaagaa ctggggcccg ccgtgcagcg tggacaccag ggtggcggcc
     7561 atcgcaatca cacaagacaa ggcggcaccc gggaaaagtt gcagcaggtg cagcggcaac
     7621 caagcggggc gggccgtgtc ggcggctgac gttggcgaca ggaaattgaa cacagcaaaa
     7681 cctctttcga acagcgcacg tgggctacgc gtgggagggt tatggccctc aatgaattaa
     7741 ataataatta aaagtcatta aagaaaaaag cggataataa cgttaataat gatcgataaa
     7801 tcaaatcagc aatccttcca gcgcctgcgc ctcaacctgc gtcagctcga agtctttctg
     7861 gccacggcgc gcggcggcag cacacgtgcg gcggccgacc ggattgcacg ctcgcaatct
     7921 gcagctagca cctcgctgtc tgacctcgag gccgctcttg gcatcgaact gttcgaccgc
     7981 atcggccggc gcctgctgct caacgaaaac gggcgcgcgc tgttgcccaa ggcccaagcg
     8041 ctggtcgaac aagcggccga agtgcaagct ctcttcatgg acgaacacgc agcacccttg
     8101 cgggtggccg ccagttttac aataggcgaa tacttgttgc cgagcttgat ctcgcattgg
     8161 acgcagctgc accccaaaag ccatgtccat ctgcgcattt gcaatacgag tgaagtgatt
     8221 accgcggtag ctggcttcga cgtcgacatc ggcttcatcg agggggcgca gacgcattct
     8281 gaccttgtcg ttcagccgtg gcttgatgac gaaatggtga tcctcgccgc gccgggccat
     8341 gagttggcga atcgctgcgc aa
//
LOCUS       DAIDFG010000126         8239 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_126_length_8239_cov_17.491813, whole genome shotgun sequence.
ACCESSION   DAIDFG010000126 DAIDFG010000000
VERSION     DAIDFG010000126.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 8239)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8239)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8239
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126_length_8239_cov_17.491813"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1430 a         2759 c         2600 g         1450 t
ORIGIN      
        1 gcgggcaccc tgtggtgctc gacaaaagcg gggtctcgct cgaactcgcc ttcggctcag
       61 acaatcgcga gccctgatcc gcttttgcct gcgctcctcg gcccagcccg acggggaggg
      121 aacgaagaga aataccaaac tccgaattcg gagagacctg acgcgcgcag cgcgtcaggt
      181 cggagaacgt ggctgtgatg tttgcacgcg gacgcaacac acgcagccaa atgaagtccc
      241 cctccatcgc ccagcggggc gagggcaggg gctaggggtg gggagttgcc cctactgtcc
      301 aaggcagacc gcggtcgcag cgtgatgccg gcgaagggag ccgtgcaaag ccgtggaatt
      361 tactcggctg tcaaatgctt cccgacaatc cccgccagct caaacatcgt cacctgcggc
      421 ttgccgaaca cagccagcag cttggcatcg gcgttgatgg cgcgtttgtt ggccgcgtcc
      481 tgcaggcctt cggccttgat gtagtcccag agtttcttga tgacctgggt gcgcgccacc
      541 ggttcggaac caatcaccgc ggccagtgcg tcgctgggtt tgaggccact gcctggcttg
      601 gcaacgcgcg gcactttcgg cttggcggtt ttggcagcgg gggctttttt ggccgcgact
      661 tttttggccg ccgccttttt ggcggcgggt ccggcagtct tcgcggccac tgttttcccg
      721 aacggcgttt tcaccgtgcc ggtccgcggc gggccggcgg tcttgcgcgg cggaaacttg
      781 gatggcgcaa actcgaagtt gaccttgccg gcttcggcgt cccaggccag catggccttg
      841 aaggggcggc gcgtgcgcat ggagacaaac ttgtcgagca aatcggtctt gccggtcgtg
      901 agcagcttga ccatctgctc gcgctccacc ggctgctgca atatcatctg gccggtcttg
      961 aagtcgcagc tcggtgtcgg ttgcgcgttg gtcggcaccg acttttcgca gacgtaattc
     1021 ttgccgtgtt cgaacacgcc cgcaccgcac ttggggcagg cccccagtga ctgctgggcg
     1081 ctgaagtcga cgatctcgcc ggtttcgccg gccttgtcgt cgccgaagtc gaactcgagc
     1141 ttgtagttct tggtctcttc gtcgaacttg atgaccatct cggcggtgaa aggccagccg
     1201 gccttggagc ggaagccgtc cagcgggccg atccgcttgt cgcgcaggaa ctgttcgacc
     1261 tcggccacct cgaaggtgcg gccggccggt gtcttgccga acgaaaagcc gcagccttcc
     1321 gcgccgttct tgccggtgca ggtgtagcgc cggtagtttt ccttgatcac gccgccgcag
     1381 ttggggcagg gcgcgctcag cgtggcgtag tcgccgggaa tcgtgtcctt gtcgtaaccc
     1441 ttggccttgt cgaccaggtt tttggtcatg gcggcaattt cggccatgaa ggtctcgcgg
     1501 ctgagcttgc cgtgttccat ctgcgcgagt ttgtattccc actcgccggt caggtcggcg
     1561 cgagagagct cttccacgcc caggccgcgc agcaaggtca tgagctgaaa ggccttggcc
     1621 gtcggaatca gttcgcggcc ttcgcgcagc atgtatttct cgttgatcag gccctcgatg
     1681 gtggccgcgc gcgtggccgg tgtgcccagc cctttttcct gcatggcttc acgcagctcg
     1741 tcgtcgtcga tcgtcttgcc ggcgccttcc atcgcaccca gcagggtcgc ttccgaatag
     1801 cgggcgggtg gacgggtctt gagtcccttg gcttcgacca cctcggcacg caccatctcg
     1861 ccgggtttca ccggcaccag gctctggccc ttgtcgcctt ccttggcatc cgccacttca
     1921 tcggcggctt ccttgccgta aatcgccagc cagcccggct tgacgagcac cttgccctcg
     1981 gtcttgaagc tgtggccgac ggcctgggaa atccgcgtgg tcaccaggta ttcggcgctc
     2041 gggaaaaaca ccgccatgaa gcggcgcacc accaggtcgt acagcttttg ctcggcgtca
     2101 ctcaggccgc tgggcgcctg cagcgttggg atgatggcaa agtgatcgct caccttgctg
     2161 ttgtcgaaaa tgcgcttgct gggcttgatg tagttgccct tgagcgccgt cgccgcatgg
     2221 ggggccaggt gtttcatgga actggcggcc agcatttcaa aggtctgctt gaccaccggc
     2281 acatagtcct ccggcagtgc acgcgaatcg gtacgcgggt aggtcagcgc cttgtggcgt
     2341 tcatacaggc tttgcgcaat cgacagcgtg gtcttggccg aaaagccgaa cttgccgttg
     2401 gcctcgcgct gcagcgaggt caggtcaaac agcagccccg cggcctgcgt ggtcggcttg
     2461 ctttcttcgg tcaccgtggc ggccttgccg cgcaccgctt cggcaatcgc gagtgcttcg
     2521 cgttcggacc agacgcggtc ggctttcaac tcggcatcgg gctcggtgtt gtcggcattg
     2581 gcggccgctt ttttccactt cgggtcaaac cacttggccg ggtactcgcc ggcctcggcc
     2641 aggaaactcg cgtggatttc ccagtagtcc ctggacacga acttgcggat tttttcctcg
     2701 cgctcgacca ccaccgacaa ggtcggcgtc tgcacgcgcc ccacggtcgt cagaaagaag
     2761 ccgccatcgc gcgaattgaa ggccgtcatg gcgcgcgtgc cgttgatgcc gaccatccag
     2821 tcggcctccg agcggcaacg cgccgcatcg gccagcggca acatctgcgc atcggtgcgc
     2881 aggctgtcaa aaccttcgcg gatcgcggcc ggcgtcatgg actgcagcca caggcgcttg
     2941 accgggtgcg cgctcttggc gtactgctgg atcaggcgga agatcagctc accctcgcgg
     3001 cccgcgtcgc aggcgttgac gagggcgccc acgtccttgc gtttggtcag cttgacgatg
     3061 gcattgagcc gcgtcttggt cttgtccatc ggcttgaggt cgaagtaggg cgggatcacc
     3121 ggcaggttgg caaaactcca tttgccgcgc ttgacgtcga actcctccgg cgcctggatt
     3181 tccaccaggt ggccgacggc cgaggtgacc agccagtcgt cgctctcaaa gtactcatcg
     3241 tgcttttcaa acttgcccgc ggtcggcgtc atcgcccgca cgatgtcctg tgcaaccgag
     3301 ggtttttctg caatcaccag cgttttcata agtacttctt caaaccttct acatcttcag
     3361 ggagcatttc cccggcttcg gcaaacagga cttttccacg cttgccactg actttcacag
     3421 ccaccaccac cggcagaccg tccggtttgg gcagcacttt ggccacttca gggctcagca
     3481 tcccggcggg aaaactgtag cccctgacct tcatatagtt gaccgccgca gcggcctcct
     3541 tatcaatcga cagcgccaga acctccaggc cccgcgcctt ctgcgcacgc cataaagcct
     3601 ccatatgggg cgactgcacc gcacaaaaag ggcaccagct ggcccaccag tacaccacaa
     3661 gcacctttcc ctgcacttgc ccgggcgtcc aggagcgtcc atccagcagg ctcaaggccg
     3721 gcaaggccag ggtgctgccg acctggggca aggggctgtc cgagcccctt gcccacgcgg
     3781 gcagccccag acccgcggcg gccagcgcgg cgacggattc gagtgccttg cggcggttca
     3841 acatggcttg ctccttgcgt gcttcactac aatagcggct tcgcgcgtac gcacgcgcgc
     3901 acccgcatgt gcgcgcacat gcatgaatta accataccaa aaaaatacgt cgtgtcaaaa
     3961 actcaggtct ctgcccccct ttccagcccg tccgtcaagc ccgcgcccga cgccggccgc
     4021 cgtattcaaa cgcgcgtgtc caacgtccac ggcaaggggg tgtttgccgt gcaggacctc
     4081 gccgaaggcg agacgctgat cgaatacgtg ggcgaagtga tcacctggaa agaagccctg
     4141 cgccgccacc cgcacgaccc gaaggacccg aaccacacct tctactttca catcgatgaa
     4201 aaacatgtca ttgacgccaa gtacggcggc aactcctcgc gctggatcaa ccacagctgc
     4261 aagcccaact gcgaggccga cgagcaggac ggccgtgtct tcatcaaggc gctgcgcaat
     4321 atcaaggccg gcgacgagct gttttacgac tacggcctga tcatcgacgc gccctatacc
     4381 aagaagctgc tggccgagta cccgtgctgg tgcggttccc ccgactgccg cggcacactg
     4441 ctcgcgccca aggacgacaa gccgggcaag aaggagaaga agaaaaagtc tgaaaaggca
     4501 gccgcggccg acaagccggt gaagggcaag cagctcagga aagagctcaa caaggaactc
     4561 aagaaagtcc tgaagaaggc cggaaagacc ggtaaagccg ccaaaccggt caaacccgcc
     4621 aaaggcaaaa agccctgagc cgtcatggcg ggcctgacca gccatccatt gccccggctt
     4681 gagcgaccca aacagttcgc ggtcatggat cccggatcga gtccgggatg acacgccctc
     4741 ccgcactgaa gcccacaacc atccccatga acacgccact caccgccccc atccgctggc
     4801 ccgccgaggc catctgggag gccgtggcgc ccgtgctgcc cggcttcacg gtcgaggtgc
     4861 tgccgcagat cgattcgacc aacaccgagc tgatgcggcg tttccggtca gccgggccgg
     4921 acgcttgcgg gcccgtgctg ctggtggccg agcagcaaac cgccggccgc ggccgcatgg
     4981 gccgcagctg gcaaagcagc gtcggcgaca gcctgacctt ttccctgggc ctgccgctgc
     5041 agccggccga ctggtcgggc ctgtcgctgg tggtcggcat cagcatcgcc gagagcctgg
     5101 aacccggcca ccgcgccgca cccgccatcc gtctcaagtg gcccaacgac ctgtggctga
     5161 gcgatgccgg cggcgaatcc aagctggccg gcatcctggt cgaaaccgcc agctggggcg
     5221 agcagcgtta tgtggtcatc ggcgtcggca tcaacgtccg accgatggcg cagccgctgg
     5281 cccctgggga cgccggccag ccggccgcgc tggcgcccgg cgccctgcag acgctgctgc
     5341 ccggcatcga cgtggcgcaa acgctgctgc gcgtggcggc gccgctggtg caggcggtgc
     5401 aggccttcgg cctgtttggt tttgcgccgt ttcaggcccg gtttgcggcg cgtgacgcgc
     5461 tcgccgggcg cgccgtgcag ctgtccgacg gcacccaggg cgtggcccac ggcgtcggcg
     5521 aaaacggcgc gctgctggtg catactgcgg ctggcatgaa agaaatcacc agctccgaag
     5581 tcagcgtccg tcccgtgacg ccccccaccg caggcagcgc gcgcccatgc tgaggctgct
     5641 ggtgttgctg ctggcgctgg ccaacgtcgc ctacttcgcc tggtcccaag gcgcgctggc
     5701 cccgctgggc atggcgcccg gcgtgcagac cgagccccag cgcatggcca cgcagatccg
     5761 gcccgaagcc atccgcctgc tgacggccga cgaagcccaa cagattgaaa acagcggcag
     5821 cgccgcccgg ccgggcaatg gcgccagcgt cgcggcggaa gccgcggccc ccgaatgcct
     5881 gcaggccggc ctgttcaacg agcagcagac cacggcgctg cgggccagcc tggaaaaagc
     5941 gctgcccgcc ggcagctggc aattcgaaag cagcaccgag ccggcgcgct ggattgtcta
     6001 catgggcaaa tacaccaccg aagacgctct ggccaaaaaa cgcagcgagt tgcgccagct
     6061 cggcgtggcc ttccgttcgg tcaacaacga gacgctggcc cccgggctgg ccctggccag
     6121 cttcacccag caggccgacg ccgacaaaga gctcgcgcgt attgccacca agggcgtgcg
     6181 taccgcccgc gtggtccagg agcgcgccga ggctcgcggg cagatcctca agcttgccgc
     6241 ggtggacgcc gccctgcgcg aacagctcga cgccctcaag ccacagttcg aaggcaaggc
     6301 gctgcggccc tgcccttgaa tcgctgcgag cccggctcgc tacatattca atagctgctt
     6361 gcgcccgatg tatatgggct gacggccgat ttgtcttaaa aatccgccaa ctcagcggga
     6421 ctcggcacgt accggccgct tgaacagctt gtgcaccagc gcgcgcatcc actggtgcgc
     6481 cgggtccctg tcggtgctgc tgtgccagac caggcgaacc tcgtaggccg gcacgccggg
     6541 aatgccccag acccggcagt cgtaacgctg gcgcaggttg cgcgcataca tttccggcac
     6601 gatggacagc aggtccgagg tcagcgcgag ctcgggcaac gcgccgaaat gccgggcccg
     6661 cagcacgatg cggtcttcca gcttcatgcg cgccagcatc tgctcgacgc tgccgtgaaa
     6721 ggtcgccgtc ggcgcggcca ccacaaacga ggcctcggcc agttgctgcg cggtcagggt
     6781 gcggccgcta cggccggagg cgggccgcca ctgcggcgcg gcgatggcca cataacgctc
     6841 gcgaaacagc agctcggtgc gtatgccgcg gtgacgcggc tgcaggatgc cgatggccag
     6901 gtcgatctcg cgcgcctcca gcgccgccgc cacgtcgctg gaagccaccg agatattgga
     6961 caggcgcgcc cccggcgcct ggctacgcat cgcgcccgcc agttccggca ggaacagcat
     7021 ttccccgagg tcggacagcg acagccgcca gtgcatgtcg ctgctggccg ggtcgaactc
     7081 ctcgtggccg gtgaccagcg tctccagcgc cagtagctgc gccagcaccg cgggcgccag
     7141 ttgctcgcac aggcgcgtcg gcttcaggcc ggacggcgcg cgcacaaaca gctcgtcttc
     7201 gaaaaagtcg cgcagccggg ccagcgcatt gctggtggcc ggctgcgaca gcgccagagt
     7261 cttgccggcc agcgtcactg agccggtgcg gtgaatggcc gccagcacgc gcagcaggtt
     7321 gaggtcgagt tgacggaaat tcatcgccgc gcctttcgcg aaagagtctg tgaagggttc
     7381 tcagggttgt cccgaattat tgatcaaaca gatgtgctcc attcatacaa tccatttgca
     7441 tgatgccttc aagctgcgta aattgcgctg caaacagaag gccattgatg cagcaagcac
     7501 ctatttttca gaccctggac gccgttgcgg ccagcggggc ggcgcatggc tgaacgttcg
     7561 ttttccgccg aggtggaaaa actgcgcctg ggcgcggggg aagagtttcg cggagaaggc
     7621 atcctggccg tcaccaaggc cctgctgcaa tcgggcgtgt cgtatgtcgc cggctaccag
     7681 ggcgcgccga tttcgcacct gatggatgtg ctgaccgacg ccaacgacat cctgcaggag
     7741 ctcggcgtgc gtttcgagca cagcgcctcc gaagccaccg ccaccgccac gctggcggcg
     7801 tccgtcaatt acccgctgcg gggcgccgtg accttcaagt ccaccgtcgg caccaacgtg
     7861 gcctccgacg cgctggccaa cctggcctcg ggcggcgtga ccggcggcgc gctgctgatc
     7921 gtcggcgagg actacggcga gggctcgagc atcatgcagg agcgctcgca cgcctttgcc
     7981 atgaagtcgc aggtctggct gatggacccg cgccccaacc tggaaagcat tgtgcaggcc
     8041 gtggaaatgg ggtttgaact ctctgaagcc agccgcacac cggtgatgct ggagctgcgc
     8101 atacgcgcct gccatgtgca tgggcgtttt cccaccaagg acaacaagcg atcggccttc
     8161 acgctcaagg acgcgatgga aaacccgcag cgcgacgcca gccgcatcgt gctgccgccg
     8221 gcgagctatg cgcaggaac
//
LOCUS       DAIDFG010000127         8192 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_127_length_8192_cov_16.472779, whole genome shotgun sequence.
ACCESSION   DAIDFG010000127 DAIDFG010000000
VERSION     DAIDFG010000127.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 8192)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8192)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8192
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127_length_8192_cov_16.472779"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1520 a         2770 c         2568 g         1334 t
ORIGIN      
        1 cagccatcgt ccagcgccgc gcaacccgtt ccacatcggg tttgtggacc gcatggaagg
       61 cgccgtccgt catgcgcacc agctgcgcga aaacctcacc ggcggcccgg ccctgcagcg
      121 ccggcggcac cggctcggcc ggcggtcgca catgcagcgc cggatgcgcc agagccgcgc
      181 tgacggcggc atggctgctg gccacccaga ggccgaggcg cgtgtcgaaa aacagcggac
      241 attcctcgcg caggctgcgg tagaagggat agggatcggg gtgggtcacc gcctcaacgg
      301 catcacgggg gaaagcggca tcacggggaa catcatggga aaaaagcatg gtcccagtgt
      361 ccccagcggc gccgtccttg tccagcgcca acagtttggc taacatcgaa ccatgcccgt
      421 ggagcccgac atcacccgca tcgccgccac catcggcgac gccagccgca tccgcatgct
      481 ggtgttgctg atggaaggcc gcgcgctgac ggccaaggag ctcgcgctgg gcgcggacat
      541 cgcgccgggc accgccacca cccatctgca gcgcctgctc gacgacggcc tgctggccgc
      601 caccgcgcag ggccgccaca agtatttccg ctttgcctcc gccgaggcgg cgcagctggt
      661 cgagtcactg atgcgtgtgg cgccaaaacg caaaagcgta cagtctccgg cgctgcttga
      721 gccgatacgc catgcacgtt tctgctacga ccacctcgcc ggcagcctgg gcaccggcct
      781 gctggccatg ctgctgcgca ggcgctggct ggcgggcgac accggtgatg cccgacagct
      841 gctggtgacg gcagcgggcg aaaaagccct gcaggccctg ggtctggacc tgtccgaact
      901 gcgcagcagg cgccggcagt tcgcctgcac ctgcctggac tggtcggaac gccgggatca
      961 cctgggtggc gcgctcggcg cggcgctggc cagccatttc acgcagcagg gctggatcac
     1021 gcgcaagaaa cactcgcgtg tggtgcaggt cacgaccgaa ggcgcggcat gcttcagccg
     1081 cctgggcctg aaactgcccg gctgaacatc cggttgatca ggcctgcagc ggcgccggca
     1141 tcacgacgat gccgtcctcg tcggcataca gccagtcgcc ggggtacacc cacacgccct
     1201 ggatctgcac cgccacaccg cgctggccct cgttgcgttt ttccgtgggc agcggcatgg
     1261 cggcaagtgc gcgtatgccg acctggcact gcgcaagctc ggcgatgtcg cgcacgcagc
     1321 cgtcgatcac caccccggcc cagccgtttt ttgcagccgc tgcaccgagg ttgccgccca
     1381 gcagggcgcg gcgcagcgag gcgccgccat cgaccaccag cacggcgggc acgcgacccg
     1441 cgggtgtttc cacgaggcca ctgccatcga ccgcagcctt gaccagcgaa ttgtcttcaa
     1501 aacacttgag ggtgacgacc ggcccgcaga agcgggaaac accgccaaaa ggccggaaca
     1561 ccgggggcag gacgcgaaat cgccccgagc tgtcgttttt atgagcgtcg cacaaatcgc
     1621 aggtggcaaa gcgtgcagtg gtcgaggggc tggtcatggg cggtgtcctt tcagggtgcg
     1681 ggtgccggcg agatgcagaa ggaaaactgc agcaagcata ccgtgcagcc gggcacctcg
     1741 tggcgcaaat gctgtgtgct ggcgcggtgt taagccagct tcctccggtg ctgggtgagc
     1801 acgtgccgct gtctgcagcg cagcctttgc cacctgcctg gccgcctctg ggatccaccg
     1861 tagcgcgccc ttgcgtactc gctgcattgc cccgggtgca gcgttttcga tcctgtcgaa
     1921 gtaaatctgc aacatccaga cccaaaaaaa ttgcgttttc cctatgaaac cgctgttttc
     1981 tccagtagca tcccccttgc cagtgaagag tagttatctg cattggtgat ttatcaactt
     2041 ctagaaggaa aatcaatcat ggcaactgca aaaaaagcac cggcaaaaaa agcagccgca
     2101 aagaaggctg caccggcaaa gaaagtagct gcgaagaaag cagctccagc gaaaaaagcc
     2161 cctgcgaaga aagcaccggc taaaaaagta gtggcgaaaa aagctcctgc aaaaaaagta
     2221 gtggcaaaaa aagcacctgc gaaaaaagcg cccgccaaaa agcgtacgcc caatgcagcg
     2281 ttcatgaagc cactgacact gagccccgcg ctgtcggccg tggttggcgc tgctgcgctg
     2341 ccccgcaccg agatcgtgcg caagctctgg gtctacatca aggccaaggg tctgcaggac
     2401 aagatcaaca agcgcatggt caacgccgac gaaaaattgc gtgccgtttt cggcaaggcg
     2461 caagtgtcga tgttcgagat ggctggcctg atcggcaagc acgtcaagta actcgacgcc
     2521 aagggcttgc cccgcgttcg cgcgggacag gaccccgtag aaagggccac gcaagtggcc
     2581 cttttttttt acgctcgatt cagaatcaaa acgaggcgca gcccttatgg gacctgcgca
     2641 tccagctact ttttttgtag cgccgcactg gtgatgttgc tcaaggtacc gcgcgtgctg
     2701 atgacctcgg ggtccagcat gatctcgatc agcgcccctt gtgtgccctg cagcgcctgg
     2761 cgcagcgcgg cttcgaactc gtcggtgcgc gtgatgcgtg tcgcgctgta gccgtaggcg
     2821 cgcgccagtg cgcagaaatc ggggttctgc agatgcgagc cgctcacatg ctgcgggtac
     2881 tcgcgctcct ggtgcatgcg gatggtgccg tacatgccgt tgttgagcag cacgatgatg
     2941 cttttggcgc catgctgcac cgccgttgcg agctcctggc cgttcatcag aaagtcgccg
     3001 tcgccggcaa tcgtgaaagc cgtgcgaccc gaaacgatgt tggccgcgat gccggccggc
     3061 acgccgtacc ccatggcacc gacggtcggt gcgagctgcg ttttcaggcc cttgaccaga
     3121 ccgtggtgcg ggaagaagcg gtgcacccag cttgcaaagt tgccggcgcc gttggtcagc
     3181 acggcatctt ccggcagcag tttcttcagc gtggcaacga tggccggcat gtcgatgctg
     3241 ccgggcagca ccacgccgcc gttggccggg tccacgttgg cgaggtaatc cgcgttggcg
     3301 gactcggccc aggcctgcca gggcaccgac acgggcgcgg tcagcacctc cagcgaacgc
     3361 gccgccgcat tcatggtggc gttgatcgcg aggtcggcct ggaagacgcg gttgagttcc
     3421 tcggcgctgg catggatgtg caccagcttc tgcttcggcc gcggcgcttc gatcagcgta
     3481 tagccaccgg tggtcatctc gcccaggcgc gggccgatgg ccaggatcag gtcggcctgt
     3541 ttgatgcgcg cggcgagttt ggggttcagg ccgatgccga catcacccgc atacagcggg
     3601 tggtggttgt cgaaggtgtc ctgaaaacgg aaggcattgc cgaccggcag cgaccagttc
     3661 tcggcaaaac gctgcagggc ctgcgcagcc tgcggcgtcc agccaccgcc gccggcaatc
     3721 accagcgggc gctgcgattg cagcagcagt tcgcgcagct tgcgcagcga gccggggtcg
     3781 ctccaggctt cgaccgcctc gacgcgcggc agcggctcgg cgtccaccat gtgggtcagc
     3841 atgtcttcgg gcagcaccag caccaccggg ccgggccgcc cgttcatcgc ggtcgcaaac
     3901 gcacgcgcca catactcggg aatgcgccgc gcatcgtcga tgcgctccac gcgtttggcc
     3961 atgcctttgg tggacggtcc gaagaagctg ctgtagtcga gctcctggaa agcttcgcgg
     4021 tcgcgcgtgt cgctggccac gtcgccgaca aacaggatca tcggcgtgga atcctggaag
     4081 gcggtgtgca ccccgatgga ggcgttggtg gcgccgggac cgcgcgtcac gaagcagatg
     4141 ccgggacgcc ccgtcagctt gccgtgggct tcggccatga aggcggcgcc gccctcctgc
     4201 cggttggtga tgaagctgat ctgcccgcgg caggcatgaa agccgtcgag caccgcgagg
     4261 taactttcac ccggcacgcc gaaagcatgg gtgacgccct gggccacaag gcattgaaca
     4321 agaagatggc cggcaagttg ttgtgtcatg ccctaattgt gccgccggaa tacacgccgg
     4381 cccaccgcgg caacgtggaa agctctctcc ataccagccg gggccgcggg actggctttg
     4441 ccagaccgca ggcgcagcgg ccccctcggg gggcaggaag ctacacgaag tgagcgaccg
     4501 tgggggccaa ttactccggc gtcttgttgt cgccgtcggc gccgccggcc tccagcgcct
     4561 ggcgggccag ctcttcctcg ctcagcggcg gcgcctcctc cagagcccgc agttcctcga
     4621 gaaactccgg cgagggctgc tcgtgctcga gcaacacttc ttccagcttc tgccggccgg
     4681 tctccacatg cacggagggt gaatgcagct cggtcggggg caccgggaat ttttcgccca
     4741 gcgtgaattt cagcgcctcg gccttctcat caacccagtg tgtgaagtca ccgtctttgg
     4801 gggtgtccga attcgtcatg accatctcct tgcatgtcgg caccagcgcg gatggccggt
     4861 ccctgctcca ttatggaggt acgcttggcg cagcgctgtc agccaatcac ctcatcaccc
     4921 gtgaatcttg cttctccttc gacaagctct gggcaaacgg tcgtaagttg ccagtcatga
     4981 tttaaggaag cctccagagc ttcaggcggc caccagcgcc gtacgcctga cccgccgcac
     5041 attgaaagcg ctggcaatca gcacaccctg catcagcgcc aggcccagca acacgccgcg
     5101 gaactgcccg tggtccatca tcacgccgaa gatcagcggc gagacagcct gcccgatgtc
     5161 caggcccgcg tagaccacgc cgtagacgcg gcccgaggcg ttgtcaggcg tcgaacgttt
     5221 gaccagcagg tcgcgcgacg gcccggcgat ccccgaggca aaccccatgg cgccaaacag
     5281 caccggcacc acccatgccg gataactgcc cagggccagc agcagcgcca ccaccgcggc
     5341 cagcccgaag ccggcgccca cgatgcgctc gcagcgcgcc gggtcggacg cgaggaaacc
     5401 gcccagcacc atgccacccg cgctgcacac catgtaaatc gtcaggcaca tggccaccag
     5461 gcccagcggc acattgtgaa gttgccgtgc ggcttcgggc gcgaaggcct gcaccacgct
     5521 gagcacaatc gcgtaccaga agaaaaaggc gaaacacatc cacaccgccg ggatcttcag
     5581 aaagcccagc gagctgtcgg ccgctggcgc gcccttgacg ggcggggcta tccggatcgt
     5641 cagcttgtca cggtgcagca ccagcacggc cagcacggca aaagccagca caccggcggc
     5701 tgccagcgcc acccgccacg aatacgcaaa cgtcagcgtc accaccagcg ccggcgccag
     5761 cgcccagccc aggctgccgg tgatgccgtg cacgctgtag gcatggccca ggcgcggcgc
     5821 gctgaccttg cggttgagca gcgtgtagtc caccgggtga aacacgccgt tgccgacacc
     5881 ggccagcacc gcgaagccgg ccatcatcca gtagctggtg ctgaccgaat aaccgaaggc
     5941 cgcaatgccc agcaggctca ggcccgcaaa cagaatcggc cgcgggccga agcggtccac
     6001 ccagaacccg gacagcgcct gcaccgcgca cgagacgaca aaaaagatcg tcatcaggaa
     6061 cccgagttcg gcataactga cgctgaactc gtccttgagc cagggaaaca gcggcgccag
     6121 cagcagctgg ctgaaatggc tgatcaggtg cgccagcccc accaggccaa tgacgctggc
     6181 atcgctgcgc aagggaacaa tgccggagcc cgggagggtg cttgatgtat tcatggcgcc
     6241 atcgtagtat cggcgctggc ttctgaaatg cgacaaaaag ccatcattca gcgaatttca
     6301 gacaaaacac gccaaccaac attcagccca tggcccgcaa gccgcgtctt catttcatgc
     6361 cggtcggcga caccgacccc ttcacgccca gccgcgagcg cccggtgcgc gtgcgggcgc
     6421 gctccatgcc ggtggactcg cacttcgagc cgcatgccca tgcctgggcg cagctggcct
     6481 actgcgccag cggcatcgtg caggtcacgg ccgggcagag ccgccacaag gccgatgaag
     6541 tcgcctacat cgtgccgccc tcgcgcgcgg tgtggattgc gccgggcgcg cggcaccaca
     6601 tcaccgtgct cgaagcggcg gaattccgca cgctgtacat cgacgccagc gccacgccgg
     6661 agggctggca cggctgccgc gtgatcgttg tctctgcctt gctgcgtgaa accatccgtg
     6721 ctctcgatgc catcggcccc cacgcttcgc tgccccccga gggggcgcat ttttccttgg
     6781 ggcggcccgg cggaaaaacc ggccccctga acccgcagcg tgagcaattg ctgacccgcc
     6841 tggtactgga cgagcttctc cacgccgaca cccaggtgct gggtgtgccc ctgccgaatg
     6901 cgcaaaccgg cgacaagcgc ctgcgcgcgc tgtgcgaggc ggtgctgcgc gcaccgtccg
     6961 aacgcgccac gctggccgag tgggccggga acataggcgc cagcgaacgc acggtggcgc
     7021 ggctgtttcg cgatgagctg ggcctgagtt accagcagtg gcgccagcag gccgtgctgg
     7081 cccacgccct gcccctgctg gcgcgcggcc agtccgtcag ccaggtggcc gccgccagcg
     7141 gctatgccag cgacagcgcg ttttctgcca tgttcaaggc ggccatgggc cagtcaccga
     7201 gtcattttca gaacaaaaac agcctctagc tcaataaagt cgggcgtaat aagctataaa
     7261 ttcaatagca aactgaccct gagccggccg cgtgccatgc ccttgacatg catcaagtca
     7321 gtgccgcagc caaggcgtag cctgagcctt ttccccgcca acacaacaca cagcacccca
     7381 caacacaaga ggcaggacac catggatttg ctcgactggg cacgcggccc cctgctcgcg
     7441 gtcgcgattg ccgtttttgg ggttggcgtg gcatggcggc ttttttcgct gtggcgcctg
     7501 ccagcgcccg caagccgccc ggcaccggcg cgccaggcct tcggcaccac cgacgccctc
     7561 aaggccagcc tttttcgcat ggcgccacac cgcggctttc atcccagcgc cacgctggtg
     7621 acgatcaacc cctatgtctt tcacatcggc ctggccctgg tcttcttcgg ttatgcgccg
     7681 cacattgcct tcatccgccg cctgaccggc ctgggctggc cggccctgcc cgacatggtg
     7741 atgtacgtcg ccgcggcggc caccatcgtg tctctgttgc tggcattgct gttccggctc
     7801 accgatccgg tgctcaaaaa aatatcccgt gccgacgaca tgctgacctg ggcgctgacc
     7861 atgctgcccc tgatgaccgg catggccgtt gtgggtgagc cctcatcggc gctgctggcc
     7921 cacgccagcc cgctgtaccg cggcccgctg gccgtgcact tgctgagcct ggagctgctg
     7981 ctggtgtggt ttcccttcgg caaactgatg cacgcctttc tggtgctgcc caaccgcatg
     8041 cagctggccc gctttctcgg acgccgtgga gtacgcacat gatcgacatg gccgttttca
     8101 aacaattcca ggaggcgatc cgccacctgg gcgagatggc gcagccggaa cccctcaccg
     8161 acgccgcccg gctgtcacgg gccaaggccg tt
//
LOCUS       DAIDFG010000128         8152 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_128_length_8152_cov_16.277510, whole genome shotgun sequence.
ACCESSION   DAIDFG010000128 DAIDFG010000000
VERSION     DAIDFG010000128.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 8152)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8152)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8152
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128_length_8152_cov_16.277510"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1565 a         2183 c         2487 g         1917 t
ORIGIN      
        1 gcgggacata cgaaacaatg ggtgtaccac ctgttgatgc agatgtggct gcattgaatc
       61 cgtgaagcat gccatctccc gcgcctaccc agacaatgct cttgcggctc ctgtacgtgg
      121 tgacaaaatc cccataccct ggaaacctcg ttccgaaaaa gtctgcgctt ggcggcgagg
      181 tgacggtggg gtttgaactc accaccggac ccatgatgga gttggcgttg cgttttcgga
      241 aacgcaggcc gttgggagtt tcatcggtcc tgctgccgcg cagccaattc agtttggcct
      301 ctgccgtggc gttggtacct ccgaaagcga tcgttttggc ggcatcggac agcgcagccc
      361 atgagaacgc gaaaccactc gagttgccga cattggtgat gatctggcgc gacgcagctg
      421 gcagggcagt caatttggtg tgagccgccc aataatagta ttcgcggtca agcgtgccat
      481 ctgttttgag gcggatatca aatttaccgt caggcttgac gataaaggtc agcaattcgc
      541 cgtgcccatc gtcgccgtca aagctggcca ggtacttcag gctgccagac tggatctggg
      601 ctgacgccac ggccggggca gtgttggagg cacccacgat ggcactgaac gccgctcgaa
      661 gggctttttc aagctcgctg ggtcgaatcg cgaggaaata gttgtccgga agaccgtcag
      721 cgccagctgt tccgtcaagc tttttggtgt cccagttggc tttatcggcc gtaggcttct
      781 caaaatagat cttggggtct gaaggtgggg ttgtgacgtt ttcgttcttg ctgaagccac
      841 cccatttggc agcgtaccag agggggtctt ccagttgtcc ggctgtgctg gtgccaaaag
      901 catattcggc tcgactctcg gcctgactta cctggcaatt cgaacaacct ccagaggcgt
      961 tgacatttgg attggtctcc catgtgccat cggctttctt tcgcaatacg gtcagggccg
     1021 ttgcatctgt atagttgaaa ccttcaatgc ccgagtggta atgcgcgcca tctttggtgc
     1081 ttcctgagat ggtgtaaccg aagccttgcg gctgattagt tgcctcgtag aaaacctggg
     1141 tgtaaacgta gagagtgctt cccaccactt cataacgaag aataccggaa acgtcgctgt
     1201 catagtcgcc gccttgttcg gagtcctccc agatgacaag aaattttcct gaagtggctg
     1261 tcggtgtgcc caccacccga aagtcgacca aggtaccgcc accaatttcg gttgcggatt
     1321 ttttcagctg ataggacggt tgaataacaa tttttcgacc ggcgattgtt ggatgtggaa
     1381 cttcaatacg aggcttgcct ggtgacagcg caaccgaaaa gcttttgact ttgaatgcgt
     1441 ctgcattggc tttgagttgt gggtctgtgg gtgtgcggac cgggttggtc ctggcccagt
     1501 aagcggctcc agccagtgcg taggagcctg aataagcggg cgcgtcgggg cacaaacccg
     1561 ttgctgtggc caaggtggag atggtttttg cggtgcaggt tgcgtcgttc gttgtgccgt
     1621 tgtttccgat gaaaaaactc ttgttgtgaa tgtcttcgtc cgtaccgatg acgttgatca
     1681 tgttcaccag gctggggctg gtcggcaagg tcgcaaacgg atcccattgg gtgccagtat
     1741 tgttgtcgta ggacgtcacg ctggcgttga aattgatggc gttaatggct cggcattgcc
     1801 cttggccaaa tatggcatca gcagccgttt ttgctgccgc gtctgcgcgg ttcaccggat
     1861 caatccacgc aggctttggg agccccatct ccgcgtcctt ggaccccgtt tgggtgtaat
     1921 cgtattggga gctgggttga agcccgccaa ggtagcgcaa ggattccgtg aaaatttcac
     1981 ccatgggatt gccccagctc gtgcactggt tgtcggtcag cgaggtgagt tggaagttgc
     2041 agctgccgtc gcctagataa gtgccatcgc tatagttgta gccatagatt cgtagcttgt
     2101 tcagggtgta gacaattcct tgggtggagg taaagctgcc atccgtactg tgattgatct
     2161 ccgttttgaa agactgcagg ttgctgcgga gtacgccgcc gctgatgttc tttgtaaaag
     2221 aaccggtcat cagaccaaac tcggccaaat ttccttctcc atattcgtgc agcaatcctt
     2281 ggggtttgaa attccccgaa gggtactgct tgcaattttc agtacccaga agggttgagt
     2341 tacaagcagc caccctgaca ataaaatccg ggccggcgcc accgataact acgccctggg
     2401 tggttttgga gggattgttt gagcctgctc ccaagccagt gatgctcacg ttattcccgt
     2461 tcggagcatt tttttcgcta ctccaataac actgccaacg ctcattggca ttccacagtt
     2521 ggtagtcgcc tcgcgcgatg cgcatcaagg gcgggttggc attggtatgg gatttcccat
     2581 tgcccgctgt gttagtgttg aatggggagt ttgcggtcag gttgtcgccg agcgtggtgt
     2641 tacagatggt ggcagaggtt tgcgtcaggt tctggatggt ccattttttg aaattgttgc
     2701 ttgggttatt gggcgtcaca aaaccatcag gagctatgac catagttacc cagccgcttg
     2761 tgctgttgac ctcgagggcg acaccctcca tataccgggc tgaattgtct tggtccaccg
     2821 ccctgacttg gtctcctacc tcaatggtaa aggtcccgtt gagggtgacc gagtgggtga
     2881 tgcagtggta ttgaggggga ggtgtgctgg gaggatcttg ggctgcgtaa taagcatcga
     2941 ccttgctttg acaagtgctg gaaactgccg ccgaaggagc cgatccgccg ttgttcctat
     3001 gaagtttgtt gtccgcagca acaaaccgga cctgtcgcct ggcgttgtcg tcgttgttgt
     3061 tgctgatggt gctggagctt gccagttggg atgcactaaa cggggtaagt tgggcaaggt
     3121 cgtcgccctt gtaatacttg gcgaatgaat gggcatccgt cggcaggctg gcccgctcca
     3181 aaacagtcag tgcggcggtg tcggtggaac ggtagcctcc ataaagcact ttgcggacca
     3241 catccacgcg tgtcatggtg gcccagttca ggaagttgcc gctccagtcg cccgtgcaat
     3301 atttgttggt gttggtgctg accgggtcaa accggttggt ggtgccgctg tagctgtagc
     3361 acttgtagga gtcgaaataa ccgtaatagt taaagctgtg cttataggtg gtttccggca
     3421 gtccgtctcc gtcgagatcg gaaaattcgt cataagccct gtaaaaaagc tggtggtctt
     3481 ttgacatgtt cagcatgatc atcggcctcg gcgtggaggt cacgatgttg ggcggaatcg
     3541 ccggcgacaa gggtgctgcg acagcaggga cttgcgaaaa agcagccgcc aaaaatagcc
     3601 cggcaatggt cagaattttt ttcatggcgt gttccttatc gcagatacgg acggaaatag
     3661 gtctgcatga caacttcagt gttgatgtcg gcaccaaagc cccgcgcggt aatgcgcatt
     3721 accttgtcgt ccgctcgggc aaggtattca atgaggtatt caggctgccg cgctacaccc
     3781 gcaatgcgtg ccacaccggt gaaggtcccc aatgggacgg agccattgaa ggtgcaattg
     3841 gtattggcgc cggtggcatc acctggtttg gcgctggccg cactggcggg gtcgccgtcc
     3901 cagcggcctt gcttggcgtt gatcgtgggc caccagggtt cagggttggt gacagttgtg
     3961 tagttgcttg agtcgtaata agacaactca aaccggcatt ggccgcgcgg gcaattggtg
     4021 tcgaaggcgg gcgaaccaag cgcgctgcct accggcctgt cagtaccgcg cgagcaaagg
     4081 gcgttgggca tgatggccga tgcattcagc aagagatcgc gctcggcatc gcgcagggcg
     4141 gcctcggttg cctgccgggc cacttcgtag tccagctggt ttctggacag ggtttcgcca
     4201 gtggtggcac gccgaatgcc cagcgtaccg aggatggtga tgaccaggag aaacatcagg
     4261 accacaggga gcgcaacgcc gccttcggcg tgacccgatg atgtttggct tcgtcgtcga
     4321 aacatgccag gcttcatcag aaggttccat tcaggttatt gcggatcgca aatacacgct
     4381 catacctttt gtacagagat cggtcagact cggcgtaagt gacggtcgcg cccctgcaat
     4441 tggtataggt gcgcaagctg ccggccttgt cctgggtcct gatgttgtcg gacgcgcgtg
     4501 tcagcacaca cacccgcact gcggtgacgc gttgccaact ggtcaaggtt cccacggtgg
     4561 gcaacgccgt cacttccgtc gccgtgtaat aagtttgtgg agaaaggttt ccggtcccgt
     4621 catgcacccc ataggcaaag gtcatgtctt caatcccctc gaagaggggt tggtacacgg
     4681 tgtcctcggc ggctttcccg ttgccgttac aggcaaggct tttggtggtg acggtttgtg
     4741 tggtgccgcc tggccccggc ttggtgtaat tcgtattggt gagtccaaac cggttggaaa
     4801 cataaaaagg ccgtcccgtc gccgcctgaa cgacgttgct gggatcgacc gcaacgctga
     4861 ctctcagaca atcgaaacca ctaacaaagg tcgctgcatc cagctcggta ttcgcaaaat
     4921 agttaatcac caccgagtca ggtgcgccgg cggtcgcggc ggggcatgcc cccgtggtcg
     4981 gattaaacac gccaccgtca cagccgaaca acccctggtt gtaagccgca aaagccgtat
     5041 tttttgtgtt gctgaaccgg cccatgagat tgaggttgac gttgctcgtg ggaacgatgg
     5101 cggggtaata cccggccatc tgcaactcgc gtccgaccat gtccagcgct agtttcccgg
     5161 tttcgttcat gcccgcaatg ttggccatcg cggtggcagt actgcgcgtt cccagatagg
     5221 cggcggcggc ggccagcgcg atcaccagac tgatgaccag actcaccagc agttcgatca
     5281 ggctcagccc gctttgcgcg ctcaaggatg tcttatggtg atggccggtt ttgatcacgg
     5341 aataatctcc aggttgacac actgaacgcc ggccagggcc gctgcggtcc ctaccgatgc
     5401 cgggcagcag ttacgggcgg caatgcctgt ggtccccacg gcgggcacag gacatatctc
     5461 gggctgccgc aaggtcgtgc cgtccaggtt gtctttgttg aaccaagcga cagtaactcc
     5521 gaaacgcaaa tcaatccctt tcacgcccgt gggcgttaca aaacccacgg cgcctgggag
     5581 ttgttgattc agatcgttgc gccagaccac caggtcatag gcagccagtt cggcgttggt
     5641 gcaagccgcg gcgttgcagt cttttgccgg ggtgaggtag gtgttgggca aaggcgtggc
     5701 ctcgatggag gcgcgttccg cagcgtatgt tctggaaaaa gtgtaggcgt ccgcgtccgc
     5761 actggcgttg gcccggatgc ggtctgcaag actgctgatg ttggaagaaa gcgcagacct
     5821 cacccatccg gtctgttcgt atttgatgga ggccgtttgc atgcccacca tgcccagcag
     5881 gccgatggaa agtacgagga tggcgaccaa ggcctcaatc aggccggcac cccgttgaca
     5941 ctttatggac atgcgctatc acccgcgtat tctttgatgg ttactcggcc tgctgagctg
     6001 atgcaaatgg accggtagtg agggctgctg acactctccg gaacataact cagggtaaag
     6061 ttggactggc cgccattagt caggcctctc gactcaaaca tgaatctccg cacggcagac
     6121 ggattggctg ctaattcaaa tgcgtccgaa cccgcctggc gaactgaaat caggctggag
     6181 ccattggtcg tcggattgtt ttgggcgcca ctgcaactgg ggttggaaaa agtaatccag
     6241 ccggcctgcc agttgctacc agttgttgca caggttggtg ttccccctgt tagcgcattg
     6301 gccgtgttgc ttgacatgca aatggtcaca cagttgtttc ggctgatggc ctcgattcgg
     6361 gctcgggcga tgtcgctgga aaattcggtg gtgatggcgg cagcctggtt gcgcaccaga
     6421 aactctctca aatttggcag gccgaacgcc aggatcaggc ccagaaccac catcgtgacc
     6481 atcagttcaa taatggtgaa acccgccccg gatccccgtg gagcaacccg aacgcggtcg
     6541 ggattggaca gtaaagggtt tgaacaggca gttgagggct gattcatggg ttcagtatag
     6601 gaacccagct tgcaatcact ggctccgacc gtcgggttcc agctaccact aatctgctgc
     6661 gatcaaggcc tgactgcggt cagtggctgc gcttctacta tggcactacc gccgcacctc
     6721 atccaccagc gtcttgaact cgtcaatatc ttcaaagctg cggtagacgc tggcaaaacg
     6781 cacgtaggcg accttgtcga gctttttgag ctcgcgcatg acgagttcgc ccaggcgggc
     6841 cgacgggacc tcgcgcaggc ccaggttgag aagtttttcc tcgatgcgct cgatggccga
     6901 gtccacctgt tcggtgctga ccggtcgctt gcgcagcgcg aggttcatgg agtcgaggat
     6961 cttgccgcgt ttgtattcga tgcggcggcc gtcctttttg acgatggccg gaaagttgac
     7021 gtcgggccgc tcgtaggtgg taaagcgctt ttcacacgcg ccgcactggc ggcggcggcg
     7081 tatgaagtct gcgtcttccg acacccgtgt ttccacgacc tgggtttcaa ggtggccgca
     7141 gaaagggcat ttcatgcagc tttgcctgaa tttttaagca ttattggcat ccagccctta
     7201 cagggcatgc gcaagcagct atcaaaagca tagcggttgc acatgcaccg gtccatcagc
     7261 ggtaaaccgg aagccggctc gtcagtgcat gaaccttggc gcgaaccgct tcgatgttgg
     7321 cagtgtcgcg cgggttgtcg agcacgtcgg cgatcaggtt ggctgtcaaa cgcgcttcct
     7381 cgtccctgaa accacgcgtc gtcatggccg gggtgccgat gcgcacgccg ctggtgacca
     7441 tcggtttttc cgggtcgttg ggaatggcgt tcttgttgat cgtcatgtgg gcgctgccca
     7501 gcacggcctc ggcttccttg ccggtgatgc ccttggcgcg caggtccacc agcatgacgt
     7561 ggctttcggt gcgcccgctg acaatgcgca ggccacgctg cgtcagggtc tccgcgatga
     7621 tctgggcgtt cttcagcacc tgctgctggt aggtcttgaa ctcgggggcc agcgcttcct
     7681 tgaaagcgac cgctttggcc gcaatcacgt gcatcagcgg gccaccctgc aggccgggga
     7741 agatggcgct gttgatggct ttttcgtgtt cggccttcat caggatgatg ccgccgcgcg
     7801 ggccgcgcag gctcttgtgc gtggtcgagg tcaccacgtc ggcgtgcggc accgggttgg
     7861 ggtacacgcc cgcggccacc aggccggcgt agtgggcgat gtccaccatg aagatcgcgc
     7921 cgacagcctt ggccactttc gcgaagcgtt cgaagtcgat gcgcaggctg taggctgagg
     7981 cgcccgcgat gatcagtttg ggtttggact cgtgggcctt gcgctccatc gcgtcgtagt
     8041 cgatctcttc cttgtcgttc agaccgtagg agacgacgtt gaaccacttg ccgctcatgt
     8101 tgagcgccat gccgtgtgtc aggtggccgc cttcggccag gctcatgccc at
//
LOCUS       DAIDFG010000129         8138 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_129_length_8138_cov_20.521094, whole genome shotgun sequence.
ACCESSION   DAIDFG010000129 DAIDFG010000000
VERSION     DAIDFG010000129.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 8138)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8138)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8138
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129_length_8138_cov_20.521094"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1215 a         2736 c         2812 g         1375 t
ORIGIN      
        1 ctggcgcccg gcaaggcttt gcaacgctgc gcatggcctt tttggccgca acccgaaggg
       61 aacgtggtga aaacagcgcc actcgttgtt gcactcctaa cccagacgcc cggtctgggt
      121 gtcgtcgcgc gcctagattg gcactgtttt caccacgttc gcacttgtat aaacagtgtg
      181 aacaggccct agctcatgcc tgccacccct gccgctgccc atctgattga ctccgcgacg
      241 gcctggcgcc gcctgctggt gaccctggcg ctgatgaccg tgggtgccgc cggcatgtat
      301 gtggtgcccg tggtgttgcc ggcggtgcag gccgacttcg gcgtggcgcg tgccgacgct
      361 gccttgcctt acacgctgct gatggtcggc tttggcctgg gcggcatgct catgggccgg
      421 ctggccgacc gtttcggtgt ggcgctgccg ctcagcgtgg gcgctgtcgg catgggcctg
      481 ggtttcggcg cagccgccct gtcgggaaac atctggctgt ttgcgctggc gcacggtttg
      541 ttgatcggcc tgctgggcag ctcggccacg ttttctccct tgctggccga cacctcgctg
      601 tggtttgtgc ggcgccgcgg cattgccgtg gcggtgtgcg ccagcggcaa ctacctgggc
      661 ggcgccctct ggccgcccat catccagcac tttgtggaaa gcgtgggctg gcgccagacc
      721 tactggggac tggccgtgtt ctgcagcctg gcgatgttcg ggctggccca gctgatgcgc
      781 cagcgaccgc cggcgctcgc tctggcgccg gtcagcgtgc gcggtgtcgc cgccagcgac
      841 cggcctttcg gtttgacgct gggtcaggcg caggcgctgc tgtgtgtggc gggcctgtcc
      901 tgctgcgtgg ccatgtccat gccgcaggtg cacatcgtgg cctattgcac cgacctcggc
      961 ttcggcgcgg cgcgtggtgc tgaaatgctg tcgctgatgc tggcctgcgg catcgtgagc
     1021 cgcctgattt ccggcgccat ctgcgaccgc atcggcgggc tgcgcacgct tctgctgggc
     1081 tcggccttgc aaaccgtcgc gctgctgctg ttcctgccgt ttgacggcat ggtctcgctg
     1141 tatgtgatct ccgcgatgtt cggcctgttt cagggcggca ttgtgccgag ctacgcgatc
     1201 atcgtgcgcg agcattttcc cgccgccgaa gccggcgccc gcgtcggcac cgtcatcatg
     1261 ttcaccatgg tgggcatggc actgggtggc tggatgtcgg gcaaggtgtt tgacctcacc
     1321 ggctcctaca acgctgcttt cctcaacggc attgcctgga acctgctgaa cttcggggtg
     1381 gtgctgtttt tgctgcgccg aacgctcagg ccgcgatttg ccgctgccta agcggcggtg
     1441 atcaggtggg actgcaaacc gcgccgtggc gacgggccga cagccggcgc ataacccagc
     1501 cgcgcccaca tctgcaccgt ataacgtggc tgtggcgctt ttagcagggc gtggatgtcg
     1561 gcataaggtc tggcctgttc cggatactcc tgcaaggcct gcgacagcgg ttgcatggcc
     1621 agcccctgcg ccgtcgccgc cagttgcgcg cgcgcatagg cccggccggc gttgacctgg
     1681 gtcttgcggt cattgccttc ggtcaccatc cagaaaaatg ccggcgtcgc ggcgatcttg
     1741 gtattgaaat ccttgatctg gccggtggtg gccgcgtcat ccggccccgg cgccttgctg
     1801 cggtcgaaca ggccgagtga cgtcagcgca cgcaccatgg gcgtgttgat ggtgaggccg
     1861 tcgcggtgct cggcaatttc gcgcgggccg acacgcagca ccttgaacga ttccagcacg
     1921 gtgcgcggcg tcaccagctc gatgcgccag gcctccatgg caatggcgcg atgcttctgc
     1981 atcaggtcag gctggctggt tcccacgaag ccggtgcgca aggcaaaggg cgcgacgctg
     2041 gcggcaatgg cctgcagcgc atctgtcgcg ggctcgcgca tctcataggc ttcgcggttg
     2101 gtgtggcgct tgaggatgtc ggcaaacagc gggtccggct tgacgccggc atctgcggcc
     2161 aaaacgatgc gtgcgacggg tcgcttgtcc agcgtggccg gaccgaactc gccttgcggg
     2221 aacagggtga tgtcggcgcg cagacctttc tgtctggcgg cgaggtccag caattccaga
     2281 aaagtaccct ggctcatcat gatctggcgc gactgcgggt cggtctcggg caacagccgc
     2341 gtgaggtcgc agtacagcat gatctcgccg ggctggcgca ggtccaccag ccaggactgc
     2401 aggttgtgcg agtgcggcgc cagaatcgcg tagctgagga tccagcgccg tacatcggcg
     2461 gtatcggccg cgggaccgtt ccaggcggca atcgcctcgg gcggcagctc ggacgagcag
     2521 ccggcgagtc cggccgtggc gcccagcacc acgccgccac cggccaagcg cacaaagttt
     2581 ctgcggttca ggttcatgag gcgctccgtt caaagactgg tgccgtcacc gtagcgcctt
     2641 ttgccgccac ctccttgcgc gaggtcaaca ggcccagctc tgccagggcc gcacgcagcg
     2701 cctcgggaaa cggtgtgtgg ggctcggcgc cggtcagggc ggccatgcgg gtgttgacca
     2761 gggtgtgcgg cgtgaaccac tggtagcgca tttcacacag cgccgcgatt ttcggcatga
     2821 ccaggcccag cgcccgcatc atcagccagg acagcgattg agtacgcagc ttgccgccgg
     2881 cgggcagcca gccgcgctcc cacgcgatgt cggtcagtgc atcgacccag tcctgccccg
     2941 tgaggctgta gccggcaaag tgcagggtct cgaaggccgg cagctgatgg cgctgcccgg
     3001 ccaccgccac aaagctgcgc gccatgtcgg gcagccaggc ccaggccgtg ggcacgttca
     3061 gggggccagg ataggtgaac ttgccgcgcc ggatgtcctt gaccatcacc tcgtccagcc
     3121 acgagccggt gccgctgcca aagaagtcgc cgccgcgaat caccaccgct ttcatgcggc
     3181 cgtcctgcgt ggccgcgcgc acctgcgctt cgctggcgat gcgcatgcgg cccttgaagg
     3241 tgctggccgc ctgcgccgtg tcttcgcgca gcaggggtgg catggattcg ccgaagttgt
     3301 agacgctggc cggcagcatc agcaaggcgc cgagttcccg cgcgacattg atggcggcct
     3361 gggtcaggcc gggcaggtcc cggcgccagg cccggtgtgt gtacaccggg ctgagcccct
     3421 gcaccaccac cgcggcgccg tgtgcggcgg cggcaagccg ggccgtgtcg ccgggctgcg
     3481 cggccagcca caccaccccg ggcatcaccg ggccgctggc gcccgggcgc agctgggcga
     3541 gcacccgcca gcccgcctgg gcaaaggcgc gtgtggccgc caggccaaac cgcccgcggg
     3601 cgccgaggat aagaacggtg gcaggctgcg tcatggaaat ctcctgaaga aaaggggtgg
     3661 acatggcgtg attgtggaaa tctgccagct gttcgacaat cccctaacgc tccacatcag
     3721 gtattcatcc atgaataaca acttcgactg gcgcctggtg cgctccttcc tggcggcact
     3781 ggaccagggc agcctgctgg gcgcggcgcg tgtgctcagc gccagccagc ccaccatagg
     3841 tcgccacatc gccgagctgg aagcacagct cggcgtgctg ctgtttgagc gcaccgggcg
     3901 tggcctgctg cccaccgcca cggcgctgca gctggccgag tcggcccgcg ccatggacaa
     3961 cgccgccaac gaactcgcgc gcaacgtgtc gggcctggag ccgggcatct cgggcacggt
     4021 acgcatcacc gccagccagc cagtggcctg ttttgtgctg ccacccatcc tggcgcaaat
     4081 gcgcctggcc ctgcccgaca tccaggtcga actggtggcc agcaacgcgg tcagcaacct
     4141 gctgcggcgc gaggccgaca tcgcgctgcg catggtgcag cccgaccagg ccagcctggt
     4201 ggtcaagcgc atcgccaggg tcacgctggg cacttatgcg caccgcgact acctgcgccg
     4261 gcgcggcacg ccgaagcaac cgcaggatct gctgaaccac gagctggtag gcagcgaccg
     4321 cgacgaggcc atcctcaagg gcatggcggg ctttggcctg ccggtcacac gcgaagcatt
     4381 tgcgtttcgc tgcgacgacc tgatcgccta ctgggaggcc gtgcgtgccg gtctgggcat
     4441 aggcttcatc gccgactacc tggccgccac cgacagggac gtcgtcgccg tgctgcccat
     4501 gatcaaggtg ccgccgctgc cgatctggct caccgtgcac cgcgagatcc gcaccagccg
     4561 ccgcatacgc gcggtgtatg actttttgag ccaggcggtg ccgaaggcgc tttagggcct
     4621 gttcacaccg cggcaggcgc tgccagcgcg cccagggccg acacccccac cggcggctcg
     4681 gccagccagg gcagcacaaa ggtgcggcgt gccaggccgc cggcaatacg cgcggtctgg
     4741 cggtgttcgc cggccaggcg ggcctgcagc agcgggtcgg tggtgccggt ggcggccacg
     4801 cttttgttga tgacccaggc ccagggctcg attttggcgc ggcgcaaatc gtcctgcaat
     4861 gccgcggcct ggctcaccgg cgtgacctcg ggcagggtca ccaggatgac gtgggtcatg
     4921 gacgcatcct gcaaccgcat gagcggcgtg atgatgtgca cggcgttgtt gctgccctcg
     4981 tactgctgca tcatctgccg gtggtaggcg ccggtggcgt ccatcagcag cagcgagtgg
     5041 ccggtgggcg cggtgtccag caccacaaag gcgctgcgcg cctcgttgac gacgcggctg
     5101 aaggcatgaa acaccgccac ctcttcggtg cagggcgact gcaggtcttc cagcagcagc
     5161 gcctggcctt gctcgtccag cgtcttgccg cgtgtggcca tgattttggc aatgtaggcc
     5221 tgggtctcgg ctttgggatc gatgcggccg accttcaggt tgggcaggct gccgttcagc
     5281 gtcccggcca catgggcggc cgggtcggtg gtggtcaggt gcacactgtg gccgcgctgc
     5341 actaggccca cggccagtgc ggcggcaatg gtggttttgc cgacgccgcc cttgcccatc
     5401 accatgatca ggccgtggcc cagggcggcc agctcgtcgg ccatgcggga caagggttcg
     5461 gccggcaagg aggccacacc cgtagctgca tcggggaccg tcgccggcat cggaccgccc
     5521 cccaacaacg cacgcagcgc cgacaggccc acggtgtcga aggcgcgcag cggcacctcg
     5581 tcgcgcggca ggcgcgccag cgcgtcgggc atttgcgcga gggcctgcag gcccagcgcc
     5641 tcgacggccc gggcggtggg gtccaccttg ccgcccgcct gcaccgtggc gtgaaacacg
     5701 ccgttgacca ccaggcgctg gttggacagc cccagggtgc gcagctcctc ggcggtgcgc
     5761 gcggcctcct gcatggggcg cggatcgggc cgtgtcacca gcaccacggt ggtgatggcc
     5821 gcattgctga gcgcggccag ggcggcgttg aagcgcgttt cctgcatctt caggcccgag
     5881 tgcgggccca ggcaggaggc gccgcggtcg ttgccggcca gaaaaccgct ccaggccttg
     5941 ggcagactga gcaggcgcaa ggtgtggccg gtgggcgcgg tgtcgaagat gacatggtcc
     6001 cagccgccgc cctcctgggc cagcaaggcg gcaaattcgt cgaacgcggc aatctcggtg
     6061 gtacaggcgc cggacagctg ctcacgcacg gtggcacgtt cgtcctcgct ggcatcggct
     6121 tccagctgcg cgagcacgcg caggcggtag gcctcggcgg ctgcgtcagg gtcgatgttg
     6181 agcacggcca gacccggcac gccgggcacg tccaccggct ggttgcgcag cggcacgccc
     6241 agcatctcgt cgaggttgga cgcggcatcg gtgctgacca gcagcacgcg ccagcccgca
     6301 tcagccagtg cgatggccgc cgcggccgac aaggaggtct tgccgacccc gcccttgccg
     6361 gtgaagaaaa gaaaacgcgt ggcggcagaa agaaaacccg gagcagaggc agtgactgtg
     6421 ttcatggcga caggatgaca caggagcgcc aggctggctt gccgcagatc aacgccaggc
     6481 cggcgacgtt cagggtctcg tggcgaaggg tttttgcggg ctgtgtaaag taggccttgc
     6541 cctgcagcgc cgcagaggcg ggacgtgtaa agttgttttg ttttttggcc agaagcaccc
     6601 acagcgcggc tgacgggttt gacaaggatt gaatgctgat accccgctgg ctccagacgc
     6661 tttggcaccg ccccggtgcg acgggccgcg tgcccggcgc cgcaccgatg gcgctgctgc
     6721 ccctgctggg atccttgctg gcgctgcctt gcgcccacgc gctggagttc agctacagca
     6781 accccttccc ggacggcccg ctggccggtg tggcgcgcag cctgagaatc agcggcgagc
     6841 tggtgccggg cgacacggcc cgcctgaccg aactggtgcg gcgcaaaccg gccgacgcct
     6901 ggcatgccct ggggcgggtc gagctggcca tttcaggcgg cgacaccgcg gaagccctgc
     6961 tgctggccga caccctggcc gccctgtacc cgcacacggt cacgagcgcc gactgcgcgg
     7021 gcgcctgcgc catcgtctgg ctggccggcg cctggcgact gttgcccgcc gggcggattg
     7081 gcctgcaaca agcgccggct tccgcgccgg gcaaggctcc caccgaccag ttgcgcaact
     7141 actttttcaa gcagggcctg cccgcgccgg tgtacgaacg ctggagggcc aaccgcaccg
     7201 acggggtgta ctggttgtcc gggcaggagg tcaacaccac cggcacctgg ccgccgtact
     7261 atttccagag gctcagcgcc cgctgcccgc cactggacag tacggacgag tcctttcacg
     7321 ccgtgcggcg ttgcgcggcc cggctggtga tcagccagaa ggcttttgcc ttcgacaagc
     7381 tgctggaggg catcaacgac ccctggtgga acgacaaccg ggacctgttg cgcaacgcgc
     7441 cgcgctgaat gtggccccca cggttgctca ctgcgtgtag cgccctgccc cccagggggg
     7501 ccgctgcgcc tgcgggccgg cagagccgga cccgcggctc tggctggtaa agaaggcggg
     7561 cggcggcgct gacccgtgcc gcgctaacat gggtgcatca ggccgggccc ggccagtctt
     7621 tctgaacatc acccaaggag tttttgtgcg atccatcctc tgcgccagcg ttttgtcatt
     7681 gtttgttttg cccgccgcct gggcgcaagc cgatgcggcc gcggccgctg ccgccaagga
     7741 ggcgggtgcg tccgtcacgg ccagcgggct ggtctaccgc tcgctgaaag acggcagcgg
     7801 cgccagcccg gccgccaccg acaccgtcaa ggtgcactac cgcggcagct ttccggacgg
     7861 caaggagttc gacagctcgt acaagcgcaa cgaaccgacc gaattcccgc tgaaccgcgt
     7921 catcccctgc tggaccgaag gcgtgcagaa aatgaaggtg ggcggcaagg ccaaactcac
     7981 ctgcccggcg gcaattgcct atggctcgcg cggcgcaggc ggcgtgattc cgccgaacgc
     8041 cacgctacag ttcgagatcg agctcctgtc ggtcaagaaa taggccccca cggtcgctca
     8101 cttcgtgtag ctccctgccc cccgaggggg ccgctgcg
//
LOCUS       DAIDFG010000130         8077 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_130_length_8077_cov_15.976564, whole genome shotgun sequence.
ACCESSION   DAIDFG010000130 DAIDFG010000000
VERSION     DAIDFG010000130.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 8077)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8077)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8077
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130_length_8077_cov_15.976564"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1331 a         2702 c         2656 g         1388 t
ORIGIN      
        1 gcgtactccc ccgcgaggac gccggcgtcg gcatcgccgc cgccatccgc ggccaacccg
       61 gcggcaggca gcaggcggca gccctggacc gcggccagcg cggcagccgc gccggcgtgg
      121 gcgctgtcgc tgaccagcag gctcaccccc gcatgaccaa gaatggccac ctgttcgggt
      181 gcggccaagc ggtaattgag cggcagcaga attgccccga cgcgggaaag cgcaaacagc
      241 agcaccagca gggccgggtc attgaagccc agccaggcca cccgggcgcc cggctgcaca
      301 ccggcagcca gcaacacccc ggtcgcgcgt tcaatacgct gccacaaggc gggataggta
      361 acgtcttccc ccgccgcatg gattgccacc tgctgcggcg aaaactgcgc gtgccgcttt
      421 atgatctgcg cgaggttcat tccgatcccg gcttacagca cggcctgttc gtcctgttca
      481 ccagcctcgt aaaggcattc acgcccgagc cggtcgatgc agagttccgc ggtccagggc
      541 agcatcagcg agccgcaacg cgaatcgcgg tacatgcgct ccagcggcag ggtcttgagc
      601 atggactggc cgccgcaggt gcgcaccgcc aagcggcaga tctcgttggc gttttccatg
      661 atggtgtagt gcgccgcgta cagcccgaga cggatttcct tggaaggatc gacgcgggcg
      721 gcgcgggcat tctgcagaaa gatggcgcgc gtctgctcca ggcgcacctt catttccgcg
      781 accgcgatct gtttggtcgg gtacatgcgg cgcttgaccg gcggcgtgcc gggaacctcg
      841 ccgcgcaggt actgcacggt gaagtcgtag gctgcctggc aaatgcccat gtaggtcggc
      901 gacagggtcg caaacatgtg cggccagcgc agcgcagcct gggcatacag gccttcggga
      961 atcaggcgct cgacctcagg cacaaacacg tcctggaaca ccagtgtgcg cgacaccgtg
     1021 ccgcgcatgc ccagcgggtc ccagtcgccg gtcacactga caccgtccga gtttttgtca
     1081 actgccaggt agagcgaatc gcgctgggtc gcacctggct tgtccagcgt gcacaaaatg
     1141 ccgtagtagt cagccgcacc agccagcgag gcaaaaattt tcttgccgtt gacgatgtat
     1201 ccgccttcga cctttcgggc cagggtgccg aaaggcgcct tgccggcggc tgcggcacca
     1261 ccttcggaaa acggctgcga gtagaccttg ccctgcttca ccacccgctc gtaatgcacc
     1321 ttgcggtgcg cctcgtggtt ggcacgctgc tcgggcgtca tgtcgaggtc gtcggcgatg
     1381 aagccggtcc acatggtgga gctcacatgc atgttcaggg tcagcgcggt ggcgccgcaa
     1441 tgccggccaa tttctgcggc ggtcatcaca taggtggcga agtcggcgcc ccagccgccg
     1501 tgttcttcgg gcacacagat gccaagcagg ccggcttcgc gcaggtcggc gtagttctcg
     1561 aaggggaaga ccgcatcgcg gtcaatctgc tgagcgcgtg gcgcgaattt ttcgcgcccg
     1621 agacgggcgg cgatggccag cagttcggtc tgcttgggcg tgagttcggt gtagtcgccg
     1681 gcaatcgaag gcggcaggta gcgggcggca ggaggtgggg agacaggcag tgcagacatg
     1741 ggtcggttcc tcgggtcggg tcagggcaga aaatgttgtt tcagaaatgt caggacagcg
     1801 tggttgaaca gctcgggctg ctcgatgttg gcgatgtggc ccacctgcgg caggcagacg
     1861 taagccgcgc cgggaatgcg gctggccatt ttttcgacca cggcgggcgg cgcgttgagg
     1921 tccagctcac cggccaggca cagggtgggc acggcgatgt tgcccagcgc agccagccgg
     1981 ttgaaggaca cgatggccga cagcgcggcg cggtaacttt ccgcggggac gcgggccatc
     2041 aacgcggcgg cttcggcgat tcgcgacggc tcggcaccgg gtgcaaacat ggtcggcacc
     2101 agttggttgg ccaggcccgc catgcccagc ccctggtcca gcggcgcaaa acggctctgc
     2161 aaaaactgct gctgccaggc gccatcggcc ttgccgaaag ccggcgaggt gctgtagaga
     2221 atcaggccgt gcaccagcgc aggatgcagg gccaccagtt cctgcgcaat catgccgccc
     2281 atgctgtgcc ccagcagcac attgcgcctg gcgcccacgt gggttatcag gtttttcaag
     2341 gcatcggcca gcagcgcgtt ggtgcagggc gacaggggtg ctgacgcgcc gtaacccggc
     2401 atgtcccagg ctaccgttct gaagccccca ctcgccaaca ccggctggtt gcgcgcccag
     2461 gcttccttgc cgccacccac gccgtgcagc aggaacacgg cggtgtcgcc ctccccctgc
     2521 tcaaaataag ccggcattcc catggctcag gccagctcgc cggtcagttg cccggcgtcc
     2581 acgatcacct gaccgtccag cgctacggtg cagccccgca atggcagatc gaaatgcccc
     2641 agcgtgtgac gccccgccac ctcgttggcg cctgtcgaat acaggaagtt gcccgcaaag
     2701 gcacgcagct cggtgccgtt gaagtccttc ttgtcgtaca gggccatcgc atcccagcgc
     2761 gccttcgggt tcatgcccca gcccacatgc gagacggcgt aggcttcgcg gtcgccccag
     2821 gcggcccaat agctgcgcat caggtcggca tccacatgct ggccggcaat gtcggtcaca
     2881 aagtcgtttt caatcgtcag caccacggct tgttcgagat aacgtttgaa ggtcaggttg
     2941 acatcgccgg gggccagcac cagccggccg ttgacactgc cctgggccgg aaaggccagg
     3001 cacaggccgc ccggccagtg cgagatggtg ccgggccggg ccgtgtaacc ccagccgccg
     3061 ccggcctgcg ccccgaccag cgagatggtc aggtcggtgc ccgcgggcga agttacacgc
     3121 atggccttgg cgttgcgcag ccgcttgatg ccggccttga ccttgggctc gagcgccgga
     3181 tcgggcaggc aacgctcgag gatttcgggg tgttcgttgg acaccatgag cacgcgcgcg
     3241 ccgcccttca gaatgtcggg cagctcggcc gcgtgcagca ggccctccac agtcaggtcg
     3301 gccacaaagg tgctggctgc gagggcctgc acgacaggcg cgagctgggc tatagcgtcc
     3361 gaggcgccgg tcgaacgcac cggcaccggc gcggccagcc ggggcgtggc cagcaccagg
     3421 tgaaaggccc gcgcaccgag gcgcgccagc gccagctcgg caagctgcac attgaccggc
     3481 cgcgactgcg tttcggacag gatggccgcc gtatcgcctg ccttgacgcc acacagcgca
     3541 aaggtctcgg taaagcagtc aatccatttg gcttcgattc gttcctgcag catgaaaact
     3601 ctttcttaaa ggcgtgagcc gagcgcgaaa tacccgccgg catgaaacag cagcggcgcc
     3661 gcctcggaca gcgtaaagcg ctcgacttcg gcaatgaaca ggcaatggtc gccggcgatt
     3721 tgccgcgaga ccgtgcggca ttcgaaccag gcgctggcgc catgcagcag cggcaagccc
     3781 caggcgtttt ccgcatgcgg cactccctcg aacttgtcgg cctggggcga ggcgaaacgg
     3841 cgcgacacct ccacctgggc ttcggccagc acgttgacga caaaacgctc ggcctcttca
     3901 aagaccggca ggctgggcga actgcagcgc agcgaccaga ggatcagcgg cggctccagc
     3961 gacagcgccg aaaacgaatt gaccgtgagc ccgacaaagg gaccgttgcc ggcgcgggtg
     4021 gtcaccaccg tgataccggt ggcgaactgg cccagcgcga tgcgcagggc ccggctgtcc
     4081 ggtgccgcaa cgggaacagg ggtaccggaa tccggggtgg cttgcagggc agcattcatt
     4141 cgtcgcataa tatatgaata ttcatgcaat acgcaagcgc atcaacccgg cctccaaccc
     4201 gacctcttct ttggcgaggc acaccgtgag ccgctcccgc aaaccggccg gtgcgccagc
     4261 ggctgccagc cggtttatcg acggctatct gtcctacctg ctcgcgcggg ccagccacac
     4321 cgtgtacagc gagttccacg cccaggtcca ggccgccggt ttgagccagc tggaatggcg
     4381 gctgctggcc accttgtcgg acggtgacgg cctgacgatc ggcgagctcg cgcgcgaaat
     4441 cctggcccag cagccgacac tgaccaaact cgtgcaacgc atggaaaaag ccggctgggt
     4501 gcggcgtggc gaagacccgg ccgatgcacg gcgcaccctg gtgttcgaga ctgaacaggg
     4561 ccgcgccacc gtggctgcgc tgctggccca ggccaaggcg cacgaaacct cgctgctgca
     4621 ggacttcagc gaacgtgacg tcacggccct gaagaagatc ctgcgcaccc tgatcggccg
     4681 cggcgacggg ggctgacccc cggttgccgg gtcggcgccg attacttgac tttgtcaagt
     4741 aattgataaa tcatggcagc catgacaccg cgtaccgctt ccacttccgt ctccgccgcc
     4801 cacgtcaagg cggttcgccg cttcaatcgc ttctacacgc agcgcatcgg cgtgctcgac
     4861 ccttatctgg gcagcgagtt ttcactgacc gaggtgcgcg tgttgtacga actggcgcat
     4921 cgcgaccagc ccacggccac cgagctgggc cgcgaccttg cgctggacgc gggctacctc
     4981 agccgcatcc tgcgccgctt cgagcgccag ggatggatgg cccgcacgcc ctcgcccgcc
     5041 gatgcccggc aaagcctgct gcaactcacc gaggccggcc accaggcttt tgcaccgctg
     5101 cagcaacgct cgcgcgacga ggcggccgcc ctgctgtcac cgctggccag gccggcgcag
     5161 cagcagctga cccaggcgct ggccaccgtg cagcgcctgc tggcgcctga cacgtcccct
     5221 gccggacaaa acgtcgtgct gcgcgacccg caacccggtg acatgggctg ggtggtgcag
     5281 cagcatggcg aggtctacgc ccgcgagtac ggctgggaca gcacgtttga agcgctggtg
     5341 gcggagatcg tcgcaggcta cgtgcgccag caccagcccg agtgggagcg ctgctggatc
     5401 gctgaggtcg acggagagcg ggtcggttcg gcctttgtcg tgcgcaagtc caaaaccgtg
     5461 gcgcagctgc gcctgctgat cctgacgccc caggcgcgcg gactgggcct gggcgcacgc
     5521 ctgaccgatg aatgcctggc ctttgcgcgc agcaagggtt acaaaaaaat ggtgctctgg
     5581 accaacagct gcctggacag cgcacgcgcc ctgtacgcca ggcgcggctt caagctggtc
     5641 aacagcgagc cctacaccgg tttcggcaaa gacctggtcg gcgagacgtg ggaactacgg
     5701 ctgtgaaccg cccgccgccc cggtgaagct caacctggcc ctgctggccg cggccgccac
     5761 cggcgtgcag gttggcgcag ccatcgtggc ctcgcgtttt gtcgtggccg acgtcccgcc
     5821 gctgaccttg gccctgctgc gttacgcgat tggttttgcc tgcctcctgc cttttttctt
     5881 gaagtctttt ctggcaccag cccttggtgg acaagcgcga gcagctatta aaaatatagc
     5941 aatttcagac tggctggcga tggccgggct cggcaccggc cagtttgcgg tgttgatcgc
     6001 gctgctcaat tacggtctgc agcacatcgg tgccgcccag gcggcgctga ttttcagcct
     6061 gttcccgctg ctgaccctgc tgctgtccag cgccctcggt cgcgaacgca tttcggccgc
     6121 gctgctggcc ggcgtcttgc tgtccatcac cggtgtggcg ctgtcgctgt ggcccaaact
     6181 ccaggcgccg cacccgggcc actggtgggg tgaggcggcg gtgctggcca gtgccggcgt
     6241 cggcgcactg tgcagcgtgt tgtaccgccc ttacctgcag cgttacccga cgctggcggt
     6301 gtccagtttt gcgatgctgg tatcggtgct gtttctcggc ctgctggccc tgaccgaaaa
     6361 ctggcccacg cgggtcatgg cgctcggcct gcaaagctgg accgtggtcg ccctgatcgg
     6421 catctccagc ggcatcggct acttctggtg gctctacgcc ctcaagcacg agtcgccgac
     6481 gcgggtcacc gtgtttctcg cactcaaccc attgacagcg gccctgcttg gcgtgctttt
     6541 tctcggtgaa gccctttccc cgtggtcggc agcagctatc tttttaatag cgtccggttt
     6601 gtggctggcg acccgcggcg cggcgccggc ccggcgataa tcgggccatg aacgcaccgc
     6661 ccggcacgca agaaaccacc cgcccgcagc cgcaatccct gaccgacctg tttgtctcct
     6721 tcaccctgct ggcgctgcag ggtttcggcg gtgtgctcgc cgtcgtgcag cgtgaactgg
     6781 tcgagaaaaa acgctggatg acgcgtgagg agttcgttga ggaatgggcc gtcgcgcaga
     6841 tcatgcccgg acccaacgtc gtcaacctct cgctgatgat tggtcaccgc tacttcggcc
     6901 tgcgcggcgc catggtggcc ctggccggca tgctgactgt gccgctgctg gtggtgttgc
     6961 tgctggcgct ggtgtatgcg cagttcgccg acaaccccgg cgtggccggt gccctgcgcg
     7021 gcatgggggc ggtcgcagcc ggactgatcg ccgccaccgg cctcaagctg atgagcgccc
     7081 tggccaggca cccgctgggc ctgccgctgt gcatcgcgct ggcgctgctg tgtttcaccg
     7141 ccatcgcctt gctgcgcctg ccgctggcct atgtgctgct tggcctcggc gggctggcct
     7201 gcacgctgac ctaccgcaag ctcgcgccat gagcctgaac ctgcaatgga cggactggct
     7261 cggcatgttt gtgcatttcc tgtcgctgtc gctgctgtcg ataggcggcg ccatcaccac
     7321 cgcacccgac atgcaccgct acctggtgga ccggcagcac tggctgaccg atccgcagtt
     7381 caacgcctcg gtggcaattg cgcaggccgc gcccggcccc aacgtgctgt ttgtcgcgct
     7441 gatgggctgg caggtgggtc tgaacgcggg cggcccgctg ctgggcctgt tcggtgtgct
     7501 ggtcaccatg ctgggcatca tgctgcccag caccacgctg acctacctcg ccgccagctg
     7561 gggccaccgc aaccgcaacc tgcgctgggt gcgcgccttc aagcagggca tggcgcccat
     7621 cgtgattgcg ctgctgatcg ccaccggctg gatcctggcc agcgccaacg tcaccggcct
     7681 gaaagactgg ccggtggcgc tgctgaccgt ggtgacggcg ctgattgtct ggcgcacccg
     7741 catgcatttg ctgtggctgc tgggggccgg cgccctgctg ggctggttcg gctggatctg
     7801 aataaaaacg ggcttgcgcc cttgcggact gggcgccaga agctatcaaa aagagagctg
     7861 ctggcgacgc gctcagctgc gctggcgcag cgcctcgtaa aggcagacac cgctggccac
     7921 cgagacgttc aggctctcga cagcgccgcg catcgggatg cggaccagct cgtcgcaggt
     7981 cttgcgtgtc agctggcgca tgccgtcgcc ctcggcgccc agcaccaggg ccacggggcc
     8041 cttgaggtcc acgtcgtaga tggttttttc ggcatcg
//
LOCUS       DAIDFG010000131         7996 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_131_length_7996_cov_18.018386, whole genome shotgun sequence.
ACCESSION   DAIDFG010000131 DAIDFG010000000
VERSION     DAIDFG010000131.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7996)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7996)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7996
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131_length_7996_cov_18.018386"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1616 a         2474 c         2528 g         1378 t
ORIGIN      
        1 gctcactgcg tgtagcgccc tgccccccga gggggccgct gcgcctgcgg gccggcagag
       61 ccggacccgc ggcccctgct tgaaggagcg ggaccccacg cttgcagctt cgtctattgc
      121 aatggcccgg atctcgcttg gctgatgtca tgaacgccct ggactccacc tccacacgcc
      181 cctacctgat tcgggcgctg tacgactggt gtaccgacaa cgggctcacg ccttatgtgg
      241 cggtggctgt ggatgaaacc gtgcaggtgc cgcgtgaata cgtcaagaac aacgagatcg
      301 tgctcaacat cagttttgat gcgacgagct cgctcaagct gggcaatgac ttcatcgagt
      361 tcaaggcgcg ttttgccggg agtgcccgcg agatcatggt gccgattggc cgtgtgattg
      421 ccatttacgc ccgcgagaac ggtcagggaa tggcgtttcc gctggcgaca gggccgtcgg
      481 agggtgttcc ccagtcaccg gttcaggcgg acgcgccgcc tgtcgcaacg gaggtggacc
      541 cgtctccagc ctcggaggcg aagatcgtac aattggttcc agccaacaat tcctcttcgg
      601 cctcttcggt gcgcgacgat gatccggaac cgcccgaacc acccaagccg caacaaggca
      661 agcgcccttc actcaagcgc gtcaagtaaa atagagtttg tgccgcttta gctcagttgg
      721 tagagcaacc gccttgtaag cggtaggtcg tcagttcgaa tccgacaagc ggcaccattt
      781 tcctccatcc tgccccggcc tgctgccagc ggacagtgtt gactccccat ttgatagcag
      841 tccggccaag gcaggctggc cttggccgtg agcaggtcag gctggctact actttcggcc
      901 gattagtatc ttcaaccgaa ttgaggtaac cttccaacct ttgtcgatca ggcgggactg
      961 gatcaggggc agcaactggc gtactttggc cgcagcggca ttgctgctga ccagcaggca
     1021 ccaggtgccg tcttcaatcg gtccagcctg aatcgcgggg cgcaaggctt caggaatcag
     1081 ggactccaca gccttgagcc ggtcgctgga ttcctggata agcccggcca gccgcgccag
     1141 cgatggcgag ttctcgacag cctggctgaa agtgacggcc tggtggagac gagggctgga
     1201 gttcaaggtt atcaggctct gattgtgaag gagacgaaca cgtgcatttg attatcacgg
     1261 atgcctggct ggcgcaaagc cgtgccattt atctgagtgg aaccaagctg gtggtggcgg
     1321 gttttgtcgc ggcactggca ttgatgctgg tggcggcagg catgtaccac tgggttttcc
     1381 tcaagggtgc gcgcgagggc tggccggtga tcggtgccct cgtcaaattg gtggtcaagg
     1441 acgagttcga acaacgggac cgcttcatgc gggaaaacct cgacgccatg gccaaacgcc
     1501 tgggcgaaat gcaggccaag atgatgcagc tcgaatcgct tggtgagcgg gtgtcgggcc
     1561 tggcaggcgt caatcccaac gatatcagga cccagcctgg gcgcggcggt gcgctggtgg
     1621 aaggacgctc gctgagcatg gaagagctgc aggcgaccct ggccgacctc gacaaattga
     1681 cagatcagcg tgtggacctg atgacggtga tggagtccag gctgtttgac cagaagatca
     1741 agaagatgat ggtgcccacc cagcagccgg tggcggccgg cgtgctcggc tccgcttttg
     1801 gctggcggat tgacccgctg aacggtcgct ccgcactcca caccgggctg gatttcccgg
     1861 ccgaacccgg cacctcgatt ctggcggcgg cgggaggcgt tgtggttacg caggaatatc
     1921 acccggcgta cggcaacatg gtcgaaatcg accacggcaa cgacctggtg tcccgttatg
     1981 gtcatgcgtc gagggtgatg gttaaaaagg gcgacctgat caagcggggc cagaaaattg
     2041 ccgaggtggg cacgaccggg cgttcgaccg ggccgcatct gcattttgag gtgctggtgc
     2101 agggcgtgtt ccaggatccc cagaaattcc tgacggcggg ccagaaactg cccgcgccga
     2161 tggcgcttgc cgctgctcct tcggccccgg gaccggccgt ccagaccgtc cggccgaagt
     2221 aagagcgcgg cagaggtaaa atctgcgctt cgcgcgcagt tgaaaccagc tggcagcgcc
     2281 cacacctaag tcgatactca ataaggattt cggccgcact gcccgctcat gggagctgtc
     2341 agcgcggcct cgcatttatg gcttccaata tcctgaccaa aatcttcggc agccgcaacg
     2401 accgcctgct caaaacctac cgcaaatcca tcgagcaaat caatgcactg gaagcgccac
     2461 tggaagccct cgatgatgag cagctgcgtg ccaagacccg ggaattcata gaccgcctgg
     2521 ccaagggcga gactttggac gccatcctcc ccgaagcctt tgccgtggtc cgggaaggca
     2581 gcaagcgtgt catgaagatg cgccatttcg acgtgcagat gctgggcggc atgtcgctgc
     2641 ataacggcaa gatttccgag atgggaaccg gcgaaggcaa aaccctgaca gcgacgctgc
     2701 cggtctacct caatgcgctg accggcaagg gcgtgcatgt ggtcacggtc aacgactacc
     2761 tcgccaaccg cgacgcccaa tggatgggca agctttacaa cttcctgggt ctgacggtcg
     2821 gcatcaacct gccgaacatg cagcgccccg agaagcagga agcctaccgt gccgacatca
     2881 cctacggcac gaacaacgaa ttcggttttg actacctgcg cgacaacatg gtctacgaag
     2941 tggccgaccg tgttcagcgc ggactgaact acgccatcgt cgatgaggtg gactcgatcc
     3001 tgatcgacga agcccgcacg ccgctgatca tcagcggcca ggccgaagac cacaccgaga
     3061 tgtacctggc catcaacaag gtggttcccc tgctgacgcg ccaggaaggc gaggccgacc
     3121 cgcgtaccgg cgagggcatc atcaagcccg gcgacttcac gctcgatgaa aagacgcatc
     3181 aggtgttcct gaccgagcag ggccatgaaa atgccgaacg catcctgttc agcctgggcc
     3241 tgattcccga aggcgccacg ctgtacgacc cggcgaacat ctcgctgatg catcacctgt
     3301 acgcggccct gcgtgccaac cacatctacc accgcgacca gcactatgtg gtgcaggacg
     3361 gcgagatcgt gatcgtcgac gagttcaccg gccgcctgat gtcgggccgg cgctggagcg
     3421 agggcctgca tcaggccgtc gaggcgaaag agggcgtgga gatccaggcc gagaaccaga
     3481 ccctggcatc gatcaccttc cagaattact tccgcctgta cggcaagctg gccggcatga
     3541 ccggaacggc cgacaccgag gcctacgagt tccaggaaat ctacgggctc gagaccatag
     3601 tgatcccgcc gaatcgcccg agccggcgcg aggaccagct cgatcgcgtg tacaaaacca
     3661 cgcgtgaaaa atacgaggcg gccatcaagg acattcgcga gtgccatgaa cgcggccagc
     3721 cggtgctggt gggcaccacg tcgatcgaaa attcggaaat catcgaccag ttgctggaca
     3781 aggaaaagct gccgcaccag gtgctcaacg ccaagcagca tgcgcgcgaa gcggacatcg
     3841 tggcccaggc cggccgcccc ggcatgatca cgatcgccac caacatggcc ggccgcggca
     3901 cggacatcgt gcttggcggc aatgtggaga aacaggttga agcggttcag gcgatggagg
     3961 atgcgcccaa agatgccaag gatgcggaaa tcgcacgcct gcgcgccgaa tgggcgcaag
     4021 agcatgaaaa agtcaaatca ctcggcggtc tgcgcatcat cgcgacggag cggcacgagt
     4081 cccgccgcat cgacaaccag ctgcgcggcc gctcgggccg ccagggtgac cccggctcct
     4141 cgcgttttta cctgagcctg gacgacacgc tgatgcgcat ttttgccggc gaccgggtcc
     4201 gcgccatcat ggagcggctc aagatgccgg acggtgaggc catcgaagca ggcatcgtga
     4261 cgcgcagcat cgaatccgcc cagcgcaagg tggaagcacg caacttcgat attcgcaagc
     4321 agctgctgga atacgacgat gtggccaacg accagcgcaa ggtgatttac cagcagcgca
     4381 acgacatcat ggacgcggcc agcctggaag ggcagatcgc ctccctgcgc gagggttgct
     4441 ttaccgacct ggtgcgccag tacatcccgg ccgaaagcgt ggaagagcag tgggacattg
     4501 cgggcctgga aaaagtgctg cgtgatgagt ggcagatgga gctgtcgctg cagggcgatg
     4561 tggaagcagc gactgccatc accgatgaag acctgctgga aaaagtgatt gccgccgcca
     4621 acgcggcttt tgccgacaag gttgaaaaaa tcggtgagga aaacttcacc cagttcgagc
     4681 gcatcgtgct gctgcagagc attgactcgc actggcgcga gcacctgagc tcgctcgact
     4741 acctgcgcca gggcatccac ctgcgcggtt atgcccagaa gcagcccaag caggagtaca
     4801 agcgggaagc cttcgagctg ttcggccagt tgctcgacag cgtgaaaaac gaagtcacca
     4861 aattgctcat gacggtccag gtccagtcgg gcgaacagct cggtcaggcc gccgaggaaa
     4921 tggaagaccg cgcggagagg atcgccaacg tgacctacac ggcgcccact gaaaccggcg
     4981 aagtggaaac cacggtggac gaggaatcgc tgcgttacaa gcaggccttg ccggacggcg
     5041 ccgtgccacg ggtgggccgt aacgatccgt gcccttgcgg ctcgggaaaa aaatacaaac
     5101 tctgccacgg caaactcaat tgagtttcgc ccgccggcct gcttctggag ccaagacaac
     5161 atgcctgtga acctgcttgc cacgcctgcc gaccagctcc atcccgtgcc cggcgtgcgc
     5221 tggggcattg ccgaggcggg catacgcaag gccggccgca aggacctgtc ggtgctgctg
     5281 ctcgatgagg gcgcttcggt cggtgcggtg ttcacacaaa accgcttctg cgccgcaccg
     5341 gtgcagattt gccgcgagca cctcgccaaa aaaggcggtc agagcctggg catacgggcc
     5401 atgctgatca acaccggcaa cgccaatgcc ggcaccggcg cggacggcct gagccgggcg
     5461 cacgccagct gcattgcgct ggcgcgcgaa ctcaatgtgg ctccggaaca gatcctgccg
     5521 ttttccaccg gtgtgatcat ggagcccctg ccgcacgaac gcatcgccgc aggcctgccc
     5581 gctgcactgg ccgatgccaa ggccgacaac tggggccggg ctgccgaggc catcatgacc
     5641 acggacaccg tggccaaggc gttttccagc cgcatcaccc tgggtggtgt acccgtgacc
     5701 atcaccggca tcagcaaggg cgccggcatg atacggccca acatggccac catgctggga
     5761 tttctggcga ccgacgcctg tgtggcgcaa ggcctgatgg cgcagctcgc caccgaactg
     5821 gccgagggct ctttcaaccg catcacggtc gatggggaca cctccaccaa cgactcgttt
     5881 gtcgtgatcg cgagcaacca ggccaggcat gcccctgtca cttcgctgga cagccccgac
     5941 ggccaggtcc tgaaggctgc catgctggat atcgcgaaaa agctggcaca ggccatcgtg
     6001 cgcgacggag agggtgccac caagttcatc acggtgaagg tcgagggcgg caaaaccgct
     6061 gccgaatgcc gtctcgtggc ttatgcgatt gcgcattcgc cgctggtcaa gacggccttt
     6121 tttgccagcg atcccaacct cggccgcatc ctggccgccg tcggttatgc cggcatcgcc
     6181 gatctcgacc agacaaaaat cgacctttac ctggacgatg tgctggtggc caaaaacggc
     6241 ggacggcatc cgggctatgt cgaggccgac ggccagcgcg tgatgaaaca aagcgaaatc
     6301 accgtgcgcg tgttgctggg tcgcggccag gccgtggaca cggtctggac ctgtgacctg
     6361 tcctacgact acgtcaagat caacgccgac taccgcagtt gagcgtccct gcttttcctt
     6421 cgtacctgat cgaccatgaa cgagcaattt gagcgcctga tggcgcgtgc ggaatccctg
     6481 ctggcgcgga ttgagtccgt gttgccgcaa ccgctgtccg cacccgactg gagccaggcg
     6541 atcgcctggc gctaccgcaa gcgcagctcc ggccacggct cgctggagcc ggtgcgccac
     6601 atcggcagca tgagcctcac ggacctcaag gaaatcgacg gccagaagga aaagatccgg
     6661 cgcaacaccg agcagttcgt gctcggcaag cccgccaaca atgtgctgtt gaccggcgcc
     6721 cgcggcaccg gcaagtcctc gctgatcaag gcctgcctca acgcctattc ggtgcgcggc
     6781 ctgcgcctga ttgaggtcga caagaccgac ctgaccgact tgcccgacat cgtcgatgtg
     6841 gtctccggtc tgagcgaaaa attcatcgtg ttctgcgacg acctgagttt tgaagaaggc
     6901 gagccgggct acaaggcgct caagtccatc ctcgacgggt ccatcgccgc ggccacgccg
     6961 aatgtgctga tttacgcgac cagcaaccgg cgccacctgc tgcccgagta catgacggaa
     7021 aacctcacct actcccgcag tgaagacggc gaggtgcatc ccggcgaggt ggtcgaggaa
     7081 aagatttcgc tgtccgaacg ctttggcctg tgggtcagct tctacccgtt cagccaggac
     7141 gaatacctga tcatcgtcgc gcagtggctg tcatcgttcg gcgtcggcca ggacgcgatc
     7201 accgcggccc aaccggcatc actggtctgg gcgctggagc gcggctcgcg cagcggccgc
     7261 gtggcctacc agttcgcgcg cgactacgcc ggccgtgaca gcgagcagcg gcccaccgcc
     7321 gcagcgccca tcgcggcata cgagcggccc ggcgacgcgg cggctgatca cgcgacatga
     7381 gtgtgctggt tgccgacggc gagcgcccgc gcgagggcgg cgccgaccgc aaggtggtcg
     7441 atgtcgcggt tggggtattg ttgcggccgg acggcgactt cctgctgacc tcgcggcccc
     7501 cgggcaaagt ctatgaacac tactgggagt tccccggcgg caagctggaa cccggcgaga
     7561 cagtggagca ggctttgcgc cgcgaattgc gggaagaaat cggtatcacg attggcgccg
     7621 tccagccctg gaaaatcgag ctggtggact acccacacgc gctggtgcgg ctgcatttct
     7681 gcaaggtgtt cgactggacc ggcgagttgc agatgcacga gggtcagcag tgtgcctggc
     7741 agcgcctgcc ggtggaggtc tggcctgtgt tgccgggtac gctgccggtg ctgggctggt
     7801 tcgcggccga acgccggttt cccggcgcga cgcacgctgc cgattgaccg gttcgtgcac
     7861 cggcggcctt gacgtgctct gttattgata gctgcctgcg cttgctgaat aagggctgta
     7921 ggcacttttg gcttgaaaat tcggcgtatc gtttgggcgc cggcctcagg ccgtgaattc
     7981 ccactgtttt ctcgca
//
LOCUS       DAIDFG010000132         7989 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_132_length_7989_cov_17.358457, whole genome shotgun sequence.
ACCESSION   DAIDFG010000132 DAIDFG010000000
VERSION     DAIDFG010000132.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7989)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7989)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7989
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132_length_7989_cov_17.358457"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1359 a         2768 c         2545 g         1317 t
ORIGIN      
        1 gccccccgag ggggccgctg cgcctgcgga ctggcaaagc cagatccgcg gccccggctg
       61 gtccggatct ggagaactca cgcttgtagc ctatggctga agtgatcgag tggctggccg
      121 acggcacgcc ttacagcccg cgttttggtg accgctaccg cagcgaactc ggtgggctgg
      181 cgcagtcgcg cgaggtgttt ttgcacggtt gcggcctgcc tgccgcctgg gccggccagc
      241 cgcagtggtg catcctggaa accggctttg gcctcgggct gaacttcctg gtgacctggc
      301 aggcctggaa ggccgacccc gcgcgaccac ggctgctgca ttttgtggcc accgaggcgc
      361 acccggccgg cgccgccgac atcctgcgtg ccgccgcggc ccaccctgaa ctcctgcctt
      421 tggccgaaca actgcaggcc cagtgctggg gcctgctgcc cggtacacac cggctggtgt
      481 tcgagggcgg ccatgtgctg ctgaccctgt gtatcggcga cacccaggcc gtgctgcgcg
      541 aacagaccct ggcagccgac gcggtttacc tcgacggctt cagccccgcc aaaaaccccg
      601 gtatgtggga cctccacacg ctcaaggcgg tggcgcgctg ctgccgccgc ggcacccgca
      661 tcgccagctg gacggtggcg cgcagcgtgc tcgacggctt ggcccagtgc ggcttcgtcg
      721 tgcagaaaac cccgggcctg ccgcccaaac gcgacaacct gcagggcgag ttcgcccccc
      781 gctgggtgcc gcgcaaggtc gccggcagtg ctcccctggc gccggtcctg ccatccagct
      841 gtgtcgttat cggcgccggc ctggccggtg cagccacggc cgccagcctg gcgcggcgcg
      901 gctggcaagt gctggtgctg gatgcgcatg agacaccggc cggcggcgcc tccggcctgc
      961 cggccggcgt gctggtgccg catgtttcgc ccgatgacag cctgctgtcg cgcctgtccc
     1021 gctgcggcgt gcgcgccacg ctgcagcagg cccaggcgct gctgcacgat ggcagcgact
     1081 ggcaggccag cggagtgctg gagcggcggc tggagagcag cggtggcctg cccgccgcct
     1141 ggcctgacgc cggccgcgac tggagcgagc cggctagcgc cgcgcaactc gaacaggccg
     1201 ggctggacac ggcatcgccg gccacctggc atcacagggc gggctgggtc aagccggccc
     1261 ggctggtgca ggcgctgctc gcgcagcccg gcatcagctg gcgcggcggc gtacaggtca
     1321 cgcggctgga ccgcgacgcc acgggccaat ggcaggtgtt cgacactcaa gagcaggtca
     1381 tcgcgcgggc cgatctggtg gtgctggccg cggcgcacgc cagcgggccg ctggtggacg
     1441 gcggcctgcc gctgcaggcg attcgcggcc aggtctcctg gggcctgcgg cgtcctggcg
     1501 acgacaaggt gtttcccgcc ttcccggtca acgggcacgg cagcttgctg cccgccgtgc
     1561 ctgtcgatga cggcgtggcg tggatggccg gcgccggttt tcagcgcgac cggaacgaca
     1621 gccggcccga cccggccgac cagcaggcca acctggagcg cctgcagacc ctgctgccgc
     1681 acgcggcgca gcaactggca gcggattttc aggacggtac cccccgggcc tgggccggcg
     1741 tgcgatgcgc ctcaccggac cgattgccgc tggtgggccc gctccagccc gtgcggcaac
     1801 cggggctctg ggtgaacacg gccatgggtt cgcgcgggtt gacgtttgcc gtgctcggcg
     1861 gcgaactgct cgccgcgcgc ctgcatggcg agcctttgcc gctggaaaac cgcatgctgc
     1921 aggcgctggc gccgcagcgc gccagcctgc tcaaacaacg ggtggcaccc gtggaccccg
     1981 tgatttgaag ccttttatag atccagccct tgatgggcct gcgcaggcag ctatatattt
     2041 agtagcaatt tcttgagctg cccggggtgc aggggttttc gcaggtagac ctgcgcaccg
     2101 gaaaaacggg cccgcagccc ctgctgccag gccggcgccg tgccggtcac aaacatgtgg
     2161 gcaatggccg gccgggtgtt gtccaccgcc ttgatcagtt cccaggggtc catgtcggtc
     2221 agccccaagt ccaccgtgac cagcaggtag gcatgggtgt tcagcagaat cagggcctcg
     2281 gtgccggtgg ccgcttcatc gacctcgtgc aggccggcgg cggccagctt ggcacgaaga
     2341 tacaggcggt catcgcgccc ggggtccacc accagcaccc gccgccccac caccggcagg
     2401 gtgcccggct ccagcgacag gggttcggtg gcgtcgccgt cgtcgagcac cacatcgatg
     2461 tcttcatcgt gggcgaggtc cagatcgatc ggcagcgaag cctggggttc aggagaagaa
     2521 ggcaggggcc ggagcccaaa ggcctcgtcc atcgcctcca gcacgcccga ccatttgacc
     2581 ggccccggaa acacccgcca ggcctgggca ggcgcgtcgt ttccgatcca gatgagtttg
     2641 aggccgtcgt gggccgggtt ggccagtgcc agcacggcct cccagctgtc cccgtcgatg
     2701 atcaggacat cgggcgcttc catcgcctcc gcggtttgtg ctgtccaggc cgaatacacc
     2761 acgtcgcgcg actcggacag gcggtacacc agatccagcg cctggcgctc ggtgccgctg
     2821 aaaccgaaga acttgacgaa tatccgcttc actgtcccgc cccttgttgt tattgccgtg
     2881 gatgatacgg ggcttgtgtc cagatgttgc tgatggcgtt gtcccgatga cacggaattc
     2941 acagtgatcc ggcccggaaa gccgcatgaa cactggcgaa taagcttatt cgcataagcg
     3001 aacaacgaat ctgtagtttg ttttcataag ccctcaccct aaagtctgcg gcttatgcat
     3061 gattttgctt ttgtatttgc gggttttgcc gtcggtctcg tggtgggact gaccggcgtg
     3121 ggcgggggct cgctcatgac gcccttgctg attttctttt tcggcgtcaa accgcatctg
     3181 gccatcggca ccgacctgct gtttgccgcc ttcaccaagc tgggcggcac ggttggcctg
     3241 gcccgggccc ggctggtgga ctggaaaatt gtcggccaga cggccgcggg cagcattccg
     3301 gcctcgctgg ccacgctgta tgtgctgcat cttgtcggac cggccagtcc cgcagcccaa
     3361 gccgtgatga ccaccacgct cggcgtggcg ctgctgctca ccgcgagcgc caccttctac
     3421 aaggccgttt atggcaaggc cgcgccgcgc cacattgacc cggctgccct gaccgcggcc
     3481 acgcaggcgc gccactgggc cctgccggtg ctgtttggcg ccgtcattgg cgccctggtg
     3541 acactgactt cggtgggcgc cggcgccatc ggggtgattg tactgatgct gctttacccg
     3601 gccctgccgc tgccgcgcat cgtggccgcc gacattgccc acgccgttcc gctgacgctg
     3661 gtggccggct tcggccacgc gtccatcggc tcggttgact ggctgctgct ggccaaactg
     3721 ctggccggct cgctgcccgg catctggctc ggctcacgcc tggtcaacag cacgcccgac
     3781 cgctggatac gctccctgct ttccgtgctg ctggcttatg ccggcgccaa actcatcgcc
     3841 atctgacccc ctgaatcacc tgatccccga ccgccatgta ccaatacacc gaatttgacc
     3901 gcgagtttgt gaaagcccgc gccgaccagt accgcgacca gctgacccgc tggcaggccg
     3961 gccagctggc cgaagacgac ttccgtccgc tgcgcctgca aaacggctgg tacgtccagc
     4021 gttatgcgcc catgctgcgc gtcgccgtgc cgtatggcga actctccagc gcgcaggtgc
     4081 gtgtgctggc gaaaatcgcc cgcgagtatg accagcccaa cgccgcgctg ttcaacgacg
     4141 cgcaggccac gcaggacaaa ctcggcccgg tcgaactgaa aagcggcctg tcggtggctt
     4201 ccaaactgac cgccggttat gcccacttca cgacgcgtca gaacatccag tacaactgga
     4261 ttccgctgga caagtcggcc gacgtgatgg acctgctggc cagcgtcaac atgcacggca
     4321 tccagaccag cggcaactgc atacgcaaca tcaccagcga cgaacgcgcc ggcattgcgg
     4381 tcgacgaaat cgccgacccg cggccctttg gcgaaatcct gcgccagtgg agcacgctgc
     4441 accccgagtt cgcctttttg ccgcgcaagt tcaagatcgc catcagcggc gccagggaag
     4501 accgcgccgc caccgcctgg cacgacgtgg gcctgcacct gatccgcaat gccgaaggcg
     4561 ggctgggctt caaggtgctg gttggcggcg gcatgggccg cacgcccatc attgccaccg
     4621 tgatccgcga gtttttgccg tggcaccagg tcatgaatta catcgaggcc atcgtgcgtg
     4681 tgtacaaccg ctacggccgc cgcgacaaca tgtacaaggc gcgcatcaag atcctggtga
     4741 aggccgaggg ccagcgctac atcgacgacg tggaagccga gtatcggcag atcatggagc
     4801 acgacggcgg cccgcacacc atcacgcagg ccgagctgga ccgcgtgacg gcctcgtttg
     4861 tgccgcccac actccagtgc gaccgcaaga gcgccgatgc caagatcgcc aacatcctgc
     4921 gcgctgccgc cgaggacgac atcgagttcg gccgctggct caagcagaac gtggcaccgc
     4981 accagaaccc cgacctgcgc gccgtgaccc tgtccttcaa gcgcctgggc caggcgcccg
     5041 gcgatgccac cgccgaccag ctcgacgtgg cggccgacct ggccgaccgt ttcagtgccg
     5101 gcgaggtgcg cgtcacgcac gaccagaacc tgctgctgcc ctgggtgcgc tgcgacgagt
     5161 tgcccgagct gtggcgcgcg gcgcgcaagt ccggttttgc gcgccccaac atccgcctgc
     5221 tgaccgacat gatcgcctgc cccggcggcg atttctgctc gctggccaat gcgcgctcca
     5281 tcccgatcgc cgaagccatc accgagcgtt accaggacat ggacgagctg cacgacctcg
     5341 gcgagatcga cctgcacatc agcggctgca tcaactcctg cggccaccac cacagcggcc
     5401 acatcggcat cctgggcgtg gacaaggacg gcaaggagtg gtaccaggtc acgctgggcg
     5461 gctccgacgg ctccacgctg agcggcgcgc cggtgcccgg caaggtcgtc ggcccttctt
     5521 tctccgcggc agaagtaccc gaagtgattg aagccgtgct cgacacctac cgcagccagc
     5581 gagaggccgg tgagaccttt atttcaacgt tgcgccgcgt cggcatggac cctttcaagg
     5641 aagccgccaa aggtgcgcgt tttgccgcca ctgctgtggc cgcctgagga aaaccgcatg
     5701 aaacttttgc cctttgccga ccacgccgtc attgaaaaca gctccgtcct ggtgctcgcg
     5761 aacgacgccg acccactgca ggcttcgctc gaaggcgtga cacgcatcga cctgcacttc
     5821 ccgaaattca ccgatggccg cgccttcagc caggcctttc tgctgcgccg ccgacgtggc
     5881 ttcgagggtg agatacgcgc caccggcgac gtgctggccg accagctcgc gcagatggag
     5941 cgcagcggct ttgacgttgc cgtgctgcgc gccgaccagg acatggcgac tgccgaacgc
     6001 gtgctggcgg cctatcctgg ttacggcgtt ggcaaatacc agggcgatgc tgtggacgta
     6061 caaccccatt tcaaggaagc agaggaaggg gctacggcct gaacaccatg accctcgacc
     6121 tcgcaaaaat caacgcagaa ctcggccaca acgcacaggg cctggtggac tgggccgtgg
     6181 gactgggccg gccagccatc gtcaccacca atttccgccc cttcgaggca gtcatcctgc
     6241 acatggtgac gcgcgccaac ccgaaagttc cggtggtctg gatggacaac ggctacaaca
     6301 ccgaggccac ctaccgcttc gccgacgaag tgacgaagtt gctcaagctg gacctgcaca
     6361 tctatctgcc gcgtcgctcg cgtgcgcacc gcgaggccgt ggaaggcccg actccggcgc
     6421 tggacgatcc gcgccatgcg gccttcactg aagaagtgaa actcgagcct tttgcccgcg
     6481 ccctgcgcga aactgcaccc caggtctggt tcaccgcctt gcgcgccacc gacaccgccg
     6541 tgcgcgccca gatggatgcg gtcagcgtca atccggatgg gctgatcaag gtcgcacctc
     6601 tcctgcactg gtcgtccaaa gagctttacc agtactgtga aatcaacggc ctgcccaaca
     6661 acttcgacta cgtggacccc accaagggcg aggacaaccg cgaatgcggt ttgcatctcg
     6721 cacactgact tatgaacgcc cgcacaccat ccaatctgct gccagccgac ccggcgacct
     6781 ccatcgctcg gctgtccaac gcccacctcg acgcgctcga ggaggaaacg attttcatcc
     6841 tgcgcgaagt ggccgcggcc ttcgagcgcc cggccttgct gttttcaggt ggcaaggact
     6901 cgctggtcat gctcaagtgc gccgagaagg ccttcggcgt gggccgcatc ccctacccgc
     6961 tgctgatgat cgacaccggc cacaacttcc aggaagtgac cgacttccgc gacctgcgcg
     7021 cgaaagagct cggcgccgag ctgatcgtgc gcaacgtcga agactcgatg aaacgcggca
     7081 cggtgcggct ggcgcatccc ggcgaatcgc gcaatgtgca ccagtcggtg accttgctgg
     7141 aagccatcga cgagttccgc ttcgacgcgc tgatcggcgg cgcgcgccgc gacgaggaga
     7201 aagcccgcgc caaggagcgc atcttctcgc accgcgacag tttcggccag tggcagccca
     7261 aggcccagcg gcccgaactc tggaccctgt tcaacacccg gctgcagccc ggcgaacact
     7321 tccgcgtgtt cccgatctcc aactggaccg aactcgacgt ctggcagtac atcgaacgcg
     7381 aagccattgc cctgccctcg ctgtactacg cgcaccagcg caaggtggtc gaacgcaagg
     7441 gcctcttggt cccggtgacc gaactgacgc cggcccgcga gggtgaggaa gtgatcgaga
     7501 aaaccgtgcg ttttcgcacc gtgggcgaca tcacctgcac ctgcccggtg gacagcccgg
     7561 ccgccaccgc cgccgagatc gtgatcgaga ccctggccgc cgatgtgagc gagcgcggcg
     7621 cgacacgcat ggacgacaag acttccgacg cttcgatgga aaagcgcaaa aaagacgggt
     7681 acttctgatg aacactactg atttgatagc tggcggcgca acactggcgg gcgctgcagg
     7741 ccaaaatgac ctgaaatccg cgctgaagtt catcacctgc ggctcggtcg acgacggcaa
     7801 gagcacgctg attggccgcc tgctggtgga cagcaaggcc gtgctgcagg accacctggc
     7861 cggtgtgcag cgcggcggcg aaaccgacct ggccttgctg accgacggcc tgtcggccga
     7921 acgagaacag ggcatcacca tcgacgtggc ctaccgctat ttcgccaccg agcaccgcaa
     7981 gttcatcat
//
LOCUS       DAIDFG010000133         7978 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_133_length_7978_cov_16.953174, whole genome shotgun sequence.
ACCESSION   DAIDFG010000133 DAIDFG010000000
VERSION     DAIDFG010000133.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7978)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7978)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7978
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133_length_7978_cov_16.953174"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1401 a         2454 c         2587 g         1536 t
ORIGIN      
        1 gcgtagccga acatcaggcc ctggtcgccg gcgccggtgt tcaggtggtc gtcagacgca
       61 tggtccacac cctgggcgat gtcgttgctc tgcttgtcgt aggcgaccag caccgcgcaa
      121 cccttgtagt cgatgccgta ttcggtgttg tcgtaaccga tgcgcttgat ggtgtcgcgg
      181 gcaacctgga tgtagtccac atgcgcattg gtggtgattt caccggccag caccaccaga
      241 ccggtgttgg tcagggtctc ggcggccacg cgcgaaagcg ggtcctgctt gaagatggca
      301 tcgaggatgg catcggaaat ctggtcggct accttgtcgg gatggccttc ggagacggat
      361 tcggaagtaa agagaaaatc gttcgccatt gcgtacactc catgaagtta gtcggcgcgt
      421 tgccggcttg gggtgcctcg gcgaacgctt tagcagtttg gtttaatgtc gccctgcaag
      481 ttgttcagta actcggcgac aacctaaatt atatcgtttt gtccatcgac aggggttccc
      541 gcccatagct gcccttcatg atcctgctgt tccgtttttt ctcccactgg cctttacgcg
      601 cgctgcatgc gctgggcgcg gccctgggct ggctggtctg gctgctcagc cccagctacc
      661 gccggcagtt tcgcgccaat gtggcgcagg cgggcatcac tttcgaggca gcccggccgg
      721 cgattgccga ggctggacgt tttgtggctg aactgcccaa gctctggatg cggccgcaaa
      781 ccgagtcatg cctgggcaat gtgcaggtcg agggccagcc gcatgccgag caggcgtttg
      841 gacgggggcg tggcgtgatt tttttcggac cgcattgcgg cagcttcgag ctgggcccgc
      901 aggcgctggc cgagctgtat ggcccgatca ccgcgattta ccggccggcc cgcaagtcct
      961 ggctggcggc gattgaacag gcgacccggc aacggccgca gctgacggtg gtgcctgcca
     1021 gcctgagcgg catccggttg atgcacaaga cactcaaggc caaccaggcc gtggccctgc
     1081 tgacagacca ggtgccgcct gaggggctgg gggtatgggc gccgtttttc ggccagccgg
     1141 cttacaccat gacgctggcg gcccggctgg cgctgcaaag cggggccgtg ttgctgccgg
     1201 tgagctgcga gcgcctgccg cgcggtgaag gctattttct caagatctgg ccgcccctcg
     1261 ccggcctgca ggcgcaggaa tcgccgccgg ccgaaggcgg cccgcgcgcc gggccattgc
     1321 aggccgagat cctccatgtt gtgacgcgca tcaaccaggc gatcgaggcc atcgtgctga
     1381 gccagccggg gcagtacctg tggggctacg cgcgttacaa gacaccacgc aaggacgcgt
     1441 tttgaagcac tcccgcccat ggcttggccg tctggtcacc cgaaccgtca tctggctgat
     1501 gcaggtgctg gcggtatttc cgctgcccct ggcgcgggcg ttcggtgtgg ccttgggctg
     1561 ggcgctgtat ggcctggtcg gggcgcgccg gcgcgtcgtg caggccaatc tgcagctgtg
     1621 tttcccttca ctcggcgagg ccgagcggcg ccagctgacc cggcagacct ttgtgtattt
     1681 tgcgcaggcc tggctggacc gtgcctggct gtggcacgcg ccgcaggcgt gggtgcagcg
     1741 gcgcgtgcgg ctgaccggcg ctgtgcacga actcgccggc aacgccccca cggtgatttt
     1801 tctgccgcat tttgtcggtc tcgatgcggc ttgggccgcc gccgccctgc attcgccgcg
     1861 ccagtcaacc accatctaca ccgaccagtc gaacaagttg gtggaccgct ggatactgcg
     1921 cggccgccag cgttttggcc acctgcggct gttcggacgg gctgacggcg tcaagcccat
     1981 cgtgacggcc ttgcgcgaag ggcagccgtt gtacctgctg cccgacatgg attttggtcc
     2041 cgacgactcc gtgtttgtgc cgttttacgg tgtgcagaca gcaaccgtgc cttcgctctc
     2101 ccgcttcgcc cgcctgggcc gcgccaaggt ggtgccgctg gtgccgcgcc tcacgtcttg
     2161 gggttacgag gtggaggtgc tgccggcctg gacccacttc ccgtcggagg atccggtggc
     2221 ggataccgca ttcatgaata cccagctaca ggcctacatc gataccatgc cggcgcagta
     2281 ctactgggtg cacaagcggt tcaagacgcg gccggaagag cagccttcgc tttactaggt
     2341 cgaacagccc agtttttcgt gtattttcgg tctccagccc ttatgggaca agcgcagaca
     2401 gctataaaaa caatagctaa cggacctggc ccagaaagtc ctgcagatag ccggccaccc
     2461 gttcgggctg ctcgtgcacg atccagtggg aggcgcccgg cacctgctgc agctgcatgt
     2521 ccggcacgta gtcggccagg ccctcgatca gcgccggcgg caaggcgata tcgtcgagcg
     2581 cccagatcac cagcgttggc agcaggaccg tcagccgctc gcgcgccagc gtgacggcgg
     2641 cagcggccgg gtcgttgtgc ggtggcccga gccggggcgg acgcaacggc gatgcacggt
     2701 acaggttgca gccgcccgtg agcccggcgc gccagacctc gcggtactgg tccctgacgc
     2761 tgtccgtgag ccaagccggc ggatggtcgc tgccgttggt caggaattgc cacaggcggc
     2821 ggaaatcgtc cttcgccagc agcgcctcgg cgtccgggcg gatcaggaaa ttcatgtagg
     2881 cactggcggc ctgctgggcc ggcgagtgct gcagctcgcg caaaaacgtg ccgggatgcg
     2941 gcgagttgat gatggcgagt tttttcatca gctgcggcat ggcggcggcc aggttccaag
     3001 cgatggcgcc gccccagtcg tgcgccacca ggcattcaag ctggggccgg tcggccggac
     3061 agactgccgc caccagcgcg gcgatgtcct gcatcaggtg tttgggccgg taggcgtcca
     3121 cctccggcgg tgcgctcgac gtcccgaaac cgcgcaggtt gggtgcgatg cagaagtaag
     3181 ccccgttctc cggcttggca aaatgcgcca gcagggtgtc ccagacaaaa gccccttccg
     3241 gaaagccgtg caggaacatc aggacgggcc ggcccggttc acccgccatg tggcaggaca
     3301 ggctgatgcc gtggggcagg gggatctggc ggatttcaac catgtcgcta tgtattcgat
     3361 agctgcctgc gcccgtttca tatgggttgc aggcagtttt tgcttgaaaa atgcacaagc
     3421 ggcagatccg gccttgccgg cggggactca ggcctcgccg gactctgatt cttcttccgg
     3481 cggcggggtg gtcaccgcct tggcggcctt gtccggcttg accggcggat gagcccactg
     3541 ccacaggtgc tgggccactt cttccacgcc ttgccgcttg agcgcagaaa acagtttgac
     3601 atcgccgccg ccagcctgca gtcgcacaat ggacagcgcc ttggcggcct cggtcttgtt
     3661 gagcttgtcg gacttggtca gcagcaccag aaacttcagg ccttcctcga cccgcgggcg
     3721 gatcacgtcc agcaggattt cgtcgagctc ggtcagcccc aggcgcgggt cgcacagcag
     3781 taccacgccc ttgaggttgt cgcgcgtctg caggtagttg cccatcacct gctgccagcg
     3841 gtatttggcg gccttgggca cggctgcgta gccgtaaccc ggcaaatcgg ccagcaccgc
     3901 atcggtcacc ccctgtttgc cgagcgagaa caaattgatg ctctgcgtgc ggcccggggt
     3961 cttggaggca aaggccaggc ggttgatctg cgtcagcgtg ttgatgcagg tggacttgcc
     4021 cgcattggag cggccgacaa aggcaatctc gggaaactca tacatcggca ggtgtttgag
     4081 ttcgggggcc gtggtcagaa acttggcggt atgcagccag cccatggcaa ttttcgcctc
     4141 ggcggactgg ggtggctggg cggccagcgc ggcggccatt ttggccgccg cttctttggg
     4201 cggcgcccgt ttggcggtcg gcttctgcgt tttaacgggt gtagatcggg gagatatgct
     4261 catatttggg attgtccccc aagccattgt aaaatcaggg agttttgcgt tggtcggcgc
     4321 cccagcgggc tattaatttc agcccaaggc tgtgcgccga ttgcctggtc caacttagag
     4381 tcaactatta aacccccgat atgaagcttg tttccacttc gttgattact tatttatttg
     4441 ccgccctgct gcttgtaccg gctgtctcgc gggcggccgg cgaggccgct ccggccaagg
     4501 tggccaagcc ggatctggtc aagggggagg ccacttacac cgcagtgtgt gtcgcctgcc
     4561 acgcggcgga cggcaattcc gcgattgccg ccaatccgaa actggcgcag caacaccccg
     4621 aatacctggt caagcagctc caggaattca agtcgggcaa gcgcaacaat gccgtcatga
     4681 aggggtttgc cagcgccctc agcgacgacg acatgaaaaa catcgcttac tgggtgacca
     4741 gcaaaaaagc caagcccggt ttcgcgaagg acaaggcact ggtgacgctg ggtgagcgca
     4801 tctaccgcgg cggcatctcc gaccgccagg tgccagcctg tgccggttgc cacagcccga
     4861 acggcgccgg catgccgtcg cagtacccgc gtctgggcgg ccagcatgcc gaatacaccg
     4921 cagcccagct gacgactttc cgcgacggtg ggcgaaacaa caacctgcag atgacgcagg
     4981 ttgccgccaa actgaacgac aaggaaatcc gcgccgtggc tgactacatc gccggtttgc
     5041 gctgaattcc ctgaaaagcc catggaatcc atggctggag ggaagggtga aacctctttt
     5101 ccgggaaaat acaggggttc gcccggtttg tattgaaccc cagccagaat aatcactcca
     5161 tacagggcgg gtcttttgca gaagacccgc cttttttgtt gccgcccaac cgaacaccca
     5221 cgcatgaccg ttcccagcca cgcagtttcc acccacggcc tgaggctcag ggcgggctca
     5281 cacgccctgc gctccaccgt ggagttgctg tcgtcgatgc gcttttccat ctcgctgctc
     5341 acggtgatct gcatcgcctc ggtgataggc acggtgctca agcagcagga gccgatcagc
     5401 aactacgtca accagttcgg cccgttctgg gcgcaggtgt ttggcgccgt cagcctgtac
     5461 acggtgtaca gcgcctggtg gttcatgctg atcctggcct ttctggtggt ttccacctcg
     5521 ctgtgcatca gccgcaatac cccgaaaatc ctcgctgacc tgaaccagct caaggaaaac
     5581 atccgtgaac aaagcctcaa ggctttcggg caccgcgccg aagcggcgct ggatgaaggg
     5641 cctgaagcgg ccgcgcggcg catcgggcag acgctggtgc agggcggctg gaaggtcaag
     5701 ctgcagcaga gaaccaccgc cggcggcgaa ggctggatgg tggcggccaa ggcgggcgcc
     5761 gccaacaagc tcggttacat tgcggcgcac agtgccatcg tgctggtgtg tgtgggcgga
     5821 ctgctcgatg gcgacctgat cgtaagggcg cagatgtggc ttaacaacaa gaccatctac
     5881 accggcggcg gcttgattgc cgatgtgccg gcccagcacc gcctgagcga gcgcaacccg
     5941 accttccgcg ccaacctgct ggtcgccgag ggcacgcagt cgagcaccgc catcctgaac
     6001 cagcccggcg gcgttttgct gcaggatctg ccctttgcgg tggagctgaa aaaattcatc
     6061 gtcgagtact actcgaccgg catgcccaag ctgtttgcca gcgagatcgt gattcacgac
     6121 aaggaaaccg gcgagaagat cccggcccgt gtcgaggtca atcatccggt ccgccacaaa
     6181 ggcatagaaa tttaccagtc cagctttgat gatggcggct ccagcgtcac gctcaaggcg
     6241 gtgccgctgg ccaccggcag ccagggggcc agcaagcctt ttgaaatcca gggcacgatt
     6301 ggcggctcca gcagcctgac gaatggtcaa ggggaaggcg ccgaaaaaat gacgctggag
     6361 tacaccgcgc tgcgtgtcat caacgtcgag aattttggcg gctcgggcag catgggtgca
     6421 acgtcgggcg cggatgtgcg caaggtcgat ttacgccagg ccctcgattc acgcctgggc
     6481 gcagccaaca agacccgcga aaagaaggaa ctgcgcaacg tcggccccag tgtgagctac
     6541 aagctgcgcg acgcgtctgg ccaggccagg gagtttcaca actacatgct gccggtcaag
     6601 atggacacgg ccgagttcag cccggcgctg tttttgctgg gcgtgcgtga taccccggca
     6661 gagccattcc agtacctgcg catccccgcc gacgaccaag gcagcatgga gggtttcctg
     6721 cgcctgcgtc gtgcgctggc cgacccggcc cagcgcgaca aagccgtcca gcgttatgtg
     6781 cgccaggcca tcgcgacgga ccggcctgat ctggcagcgc agctgtccgc gtccgcgtcg
     6841 cgggcactgg ccctgttcgc cggccccgac cagccggtgg ccggagccca ggccaggccc
     6901 ttggccggac tgcaggcggt ttccgatttc atggaaacca acgtgccgga agacgagcgc
     6961 agccgtgccg gagaagtgct ggtgcgcatt ctgaatggcg tgttgtttga actggtgcag
     7021 ctcacgcgtg agcaaagcgg cctgcagccg ctgccgcgcg acgaaaaaac ccaggctttc
     7081 atggcacagg ccgtgttgtc tctcagtgat gcccaccatt acccggcgcc catggcgttt
     7141 gaactgaagg acttcacgca gctgcaggcc agtgtgttcc aggtggcgcg tgcccccggg
     7201 agaaacattg tctacctcgg ttgcgccttg ctgattctgg gcatctttgc catgctttat
     7261 gtacgcgaac gacgcctctg ggtctggctg gcgccggccg aagacggtgc ggattccatg
     7321 gaacccggaa aagagcttga aaaaaagggc ccagaatctg gcgcaggcag ccaggcgacc
     7381 atggccatgt cgaccaatcg ccagaccatg gacggcggta aggaatttga aatgctgaaa
     7441 acaaaacttt tacaggtgcc ccgatgaaca ccaccaccac cctgaactcg cgcaccacgg
     7501 acgcgaccac catcagcttg cagcaaggct atttcgccaa gcgcaatgcg ctggactgga
     7561 tttttgcggc cattgcgctg gccggcgggc tgtatgccct tgcccgttac ggcgctttca
     7621 tggatgtcta tgaaaaaggc attttgctgg gcgcgattcc tgccgtgatc gcgatgggct
     7681 ggttctggcg gccgctgcgc gcgctgatgc tgctggtagc gggcttttcg ctgctcggta
     7741 tcgtgtccta ccagggtgat ctggcacgcg ccgaacaggt gttctggctc aagtatttcc
     7801 tgtccagcca gtcggccatt ctctggatga gtgtgctgtt tttcatgagc acggcgttct
     7861 actggatggg catgtttgcc cgggggcaga gcggcacgat ggagttgctg ggctcgcgga
     7921 ttgcctgggt ggctgtgggc atggcactgg tcggcaccat ggtgcgctgg tacgaaag
//
LOCUS       DAIDFG010000134         7957 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_134_length_7957_cov_18.003543, whole genome shotgun sequence.
ACCESSION   DAIDFG010000134 DAIDFG010000000
VERSION     DAIDFG010000134.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7957)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7957)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7957
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134_length_7957_cov_18.003543"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1549 a         2610 c         2500 g         1298 t
ORIGIN      
        1 cctgatccag aagaacagac acatcaagct ggccggcaac gacaagctgc gcatcgaacg
       61 cgaactcaag gaacccaagg accgggcgca aatggttcgc gacgtactga aatcactcgg
      121 acaacccaaa accaccacgg aagccacgcc cgcatgaccc ctgctgaaac cctcccaggc
      181 ctagccgtca aactcaccac gcccgacctc aaactcggcg acttcaaact gatcgccttc
      241 gacatggact cgacgctgat caacatcgag tgtgtcgatg aaattgccga cgccgccgac
      301 cgcaaggccg aagtgtccgc catcaccgaa gcggccatgc gcggtgaaat tgccgactac
      361 aaggaaagcc tgcgccggcg tgtggccctg ctcaagggcg tcagcgtcga cagcatggac
      421 gaggtctacc accagcggct caagctcaac cccggcgccg ccgaactggt gcatgcctgc
      481 aaggcggccg gcctgaaggt gctgctggtc agcgggggct tcaccttttt caccgaccgt
      541 gtgcgtgacg agctcggtat cgactacacg cgctcgaacg ttctggaaat cgagaacggc
      601 ctgctgaccg gccgcatggt ggaccagccc tggggggaca tctgcgacgg cgaggaaaag
      661 aaaaaaatgc tgctgcaaac ctgcgccatg ctcgacatcg ctcccgccca ggccatcgcc
      721 atgggcgacg gcgccaacga cctgcccatg atgggggtgg ccggcctctc ggtggcctac
      781 cacgccaagc cgaaggtgcg cgaacaggcc atggtcgcca tcaacgaggg cgggctcgac
      841 cgcctgctgg aagtgttcag ccagccagcc ggcacagcgg ccccgcaaca ggcctgaagc
      901 ggctagccta ttgagcgttt caaaactcag gacagtcgga acgctcaatc cctcaccccg
      961 accctctccc agagggcgag ggtgagagca gtccaattca ctgtcgtgta tttggaaaaa
     1021 atctatagcc tgtttgggct acagcagggc gggctgaacc agcgcgtcct gtgtctggag
     1081 cctcaacggc aggcgcaccg tgaaacaggt gcctgaaccg gccgggccca ccaggctgct
     1141 gacttcaatc gtgccaccgt acaggtccac cgcgttcttg acaatggcca ggcccaggcc
     1201 ggtgccggga atggcgccga cattgctggc gcggtgaaag gactcgaaca ggtgggtgat
     1261 ttcatcggcg ggaatgccga tgccgttgtc cgacacttcc agcaccagtt gccggtccgc
     1321 cagaaaaact cggcacagca ccaccccgcc cagcggcgaa tacttgacgg cgttggacag
     1381 caggttgccc aggatgtgcc tgagcagttt ttcgtcgtgc agccccgccc caccgtcggc
     1441 cgcaaacagc aggcgcagct cgcacgggct gccagggtgc agcgcgcgcg cctcctgcac
     1501 cagctgctgg cagagttttt cgaggtccag gggccgcggt tcgaactcca gcatctgcgc
     1561 ctcggccttg ccgatcagca gcacgcggtc gagcatgcgg gtcatgcgtt gcacactggc
     1621 cgcaatggtg tcgagcactt ccgccttctc ggtggcgggc aggcgctcac cataatgttt
     1681 gaggatttcc cccgacgaca ggatgctggc cagcggcgtg cggaactcgt gactggtcat
     1741 ggccacaaaa cggctgcgca gttcgttgag ttcccgctgc cgctccagcg cctcgcgcgt
     1801 gtcctgttcc gatttcttgc gctcggtgat atccaggaaa gtccagatca ccccggcctc
     1861 cggctcaagc ggccgcaggc agttgccgcc cgcctccacc cagaaaagct cgccgttgcg
     1921 ccgctgaaac tgctgctcgc aggtgtagct gccggtgcct ctcagggctt cgcgcgcacg
     1981 ctgcccgaag ttttcccacg cggctgcgtc cgtgtgcagt cgcagggccg actggccgat
     2041 caggtcctcg cgcggatagc ccatcatctc ggcgaacttg gcgttgaccc actccagcct
     2101 gcgcgacacg gacagcacaa tgcccaccag cgcgctgttc aggatggctt cccgctccga
     2161 cgaggtacgt atcagctcag cttccagctc cttgcgccgg gtgatgtccg tcatcaccca
     2221 caccgtgccc tgcgtcaggt cgcgcgggct gacggccttg ccggacagcg aaatccagat
     2281 gtgtgaaccg gtgtactggc ggacctggat ctcggtttcg taagcccggc cctcccggat
     2341 gcaggctgcc acctcgcccc cgacgcgcag gtagtccgcg cgcgacaggt aaaacggctc
     2401 catcgaggtc agtggctggt cgccggcggc aaaaatttcg agcatggcct ggttggccca
     2461 gcgaaagcgc ccttccggcg tcagaaacgc aatgccgacg atggagttgg ccagtatggt
     2521 ttcgcgctcc tgcagcaccg cgcggtaacg ctcctgcgac tggcgcaggg cttcgaccgt
     2581 ggcctggtcg gcctggctgg cggcctgggc ctgttctttt tcggccagcg ctacattgat
     2641 gcggtcggcc agcgcaaacg aaagcagcac catctcgagc gccgaaccga tcagcagggc
     2701 attggaggtc agcaggttgg tcggcaggat gcccgtgttg tggagcacaa aggtcgccac
     2761 ccccaacaac aacaccgccc acgccgtcag gaaataacgt gcgccgggaa atttctgcag
     2821 catgctgaac acgccggcca gcacaaccac gggaacactg acgacagcca gcccggtcac
     2881 catccacgtc gaaaccgtgt agggcagcag cagcgcgacc agcagcgcca gcagccaggc
     2941 gccggtcagg gccagcatca cgcggttgat gcgcggcatg cgcacggcac tgttgaggaa
     3001 gttgcgtgcg aacaggagcc cgaaagcggc cgccgtggcc atgcccaccg gcggcgacac
     3061 accgttccac caggtctgtc caggccagag caactgcccg cccagtcccg tcaacgcggc
     3121 ctgcgacacg gccatgccac cgacaaaacc gacataaatc aggtaggccc ggtcgcgcac
     3181 cgaaaaatac agcagcaggt tgtacagcag cagaccgatc agcaggccaa agtagagggc
     3241 gagcagcgcg tactcccgct ggtcgtgctg ccacagtgcc ccggcctgcc acaggtgggt
     3301 cggaaccgac agcgttccct gcgactgcac acgaaggtaa agcgtggttc cagcgccggg
     3361 ctgcagcatc accggcaaca catggttgcg gtgcggcacg gcccggctgg caaagggcaa
     3421 caggtccccg ccgcgctggt gttcaaaccc tccgcgggga gtcgccgtgt aaaggtccag
     3481 ctggtcgatc gccggatagg cgacctccag cagccacggc gccggtgtat cggtctgcgc
     3541 ctgcaacgtg atgcgcagcc agaaggccga cgaggtcagg ccgaagttgg tggctgaccc
     3601 gctctgggcc accgttttga agcgcgcctg ctgcgccggc tgcagcacct cgtccagcga
     3661 cagctggccc cctgggtctt ccagcaccat gaaggcgcgg gacagagtgt agctgccctg
     3721 ctggccgatc atcagcgcgc cgtcctgtgc atgcagcagc ggggagatca gcagcgccag
     3781 catcagcggg aaaccgcgca accacgcacg cgggcggaca aggaatcgga agcaagggag
     3841 gtcaggcatg agcgtggcgg aaagcccctt tttggcgccc attatccacc gcacacgcgc
     3901 cagcgagcgc tggcgaatcc tgcctatgga caaaacccct ggggctggtt agtgtgacaa
     3961 cagaccccga aggaaataca ccatggccct ggaccacgcg caaccgctcg acatcatcaa
     4021 tgtgcagccc ctcgccggca agctgcagga tgtcaaaacc cacagcctgc tcaagaccgg
     4081 gaaactgcag ttgatgcgcc tggtgctggc ggccgaccag agcgtgcccg aacaccaggt
     4141 gccgggcgaa atcaccatcc agtgcctgga ggggcaagcc gtggtcagca cccccggact
     4201 gtcgcgcacg ctgggtgccg gtgaactgat ggccttgtcc ggcggcgaac cgcatgcgct
     4261 gaaagccacc acagccgcct cgctgctggt caccattttg ctgaacccat gaatcgctga
     4321 agccaggcgc ccgcgtcgcg aagaccggaa ttctttggaa tgctatttat ttgatagctg
     4381 cttgcgcttg cgggaaaaag gctgcgagga ctttttggca gaaactgtga acatgcgccc
     4441 cgcaagccca ggcattagtc atcgagcctg cgggaacggc acgtcacgga cggttttggc
     4501 catgcagttg gcgaaggtgc aggcgccgcc tctgcaaaaa agtctagttc aacaggccga
     4561 acgccagctg caagtgggcc gctttcaggc gcgcgtgggc cgcgtccagc gcggcgaagg
     4621 ccgcgtcgat ggctcgccgg tcgggcaccg gtgcgcgcag cagcatctcg taggttgccc
     4681 gtagaaaatc gtagtcagcc agggcaacgg tgggacgcat gtgagaagtg gtgaggggtg
     4741 cctgcatttc atctccggct tgctggatcc acagtgcagg gtccgctgat ccggcccctt
     4801 cgtgccattc attgtgagca agccagagca ctgacgacat aggtcaactc aatctccggt
     4861 tgtcggagac cacctacccg ccagccatcg tgcggccacg gcgtcagtcc aggcgaatgt
     4921 cgtcaaagca gtcctgcaca gggggctttc agcaggtgac cggtcggccg tggcgtggtg
     4981 ccacgattgc aggcacccgg ggatcagcgg gtcatgggcc acgggcgcgc caaggctgct
     5041 acagcggacg cagatgggtc ggtgggtcga agtcgcccat cgccagcttg aagcggatat
     5101 gggcttcatc catggccacc atcgcgtcgt tgaccgcctt gtagtcgggc gggtccatgc
     5161 tcagaaaacg catgtagtca gcccgcgcct cgtcatagtc ggccagggcc tgctgggctt
     5221 tcgtgagggg agcagacatg gccggttaaa tggcgggctg aaccgtaccg ggctcttccg
     5281 gatgcgccgg ggcgggcacg ggaccgaggt caggctcgac cggaaattca acaggcgcag
     5341 gcacggtttt gccgccaggc gacaggggct caccattttc ggaaggatgg gggcgatggg
     5401 tggccgacat gggggggggt caaaacggag cgatccaggc gttcaccgga acctccgcag
     5461 tagccagggc gaccacggcg gcgatgccca cgaacaccgc caaaacataa gctaccaacg
     5521 accccagcac acgccggggc ggtgtgctgg agaaccaggc aggctggtga tggtgggtgt
     5581 ctgaatggat gaccatgctg tgatcttcca tgcaacgcac cactcccgcc ataggcgaaa
     5641 tctgaaaagc actgtcagcg ccggctgaca aggcgagccc acggcaagcg ccacgtgccg
     5701 ttcatcgctt cttttttgat cgctgcctgc gcaatcccca caggggctgg aggcggattt
     5761 gtctgcatag acagtaccac cgggcggtga aaacctggga ctagagcttg ggaatacgca
     5821 gcgtcttgct cagcatcagg gtgccggaca acacaaacag cagcgacaag ggatgcaacg
     5881 cccaggggcc agccatccag acaccgccgt agatctggtc acccaggcgg tcctgccagg
     5941 ccgccacggc cagcaaggcg gtcagcagca cgctggtcgg aatcggtgtg ccctcgaaat
     6001 aggccacctt ggcggctccc gccgacagcg tttcggccgt gacgttgtac cgtgccagcc
     6061 ggctcacgcc gcagcagacg aaatagatca gcgcaatcca gtcccagccg ccacgcaggc
     6121 ccgccgcaaa cgccagtgcg gccggcgcca ccccaaagga aatgatgtcc gccagcgaat
     6181 cgagctcccg ccccaacgcc gaatgctggc tgcgccagcg cgccacgcgt ccgtcgagca
     6241 cgtcaaaaat aaatgcggcg ggcgccatgg ccgccgccgt ataaaaccag gccttggccc
     6301 cctcgaccgc atacagcatg gccaggaaaa cacccgcaac gccgcaggcc gcattacaca
     6361 gcgtgaggaa gtcagcgagg tggaactccc gcagcatgga gaaatgtctg ggcttgtcag
     6421 tcaagggtgc ctgctttcat tgggggcatg tcggaggcaa ttctgccgga cttatttctg
     6481 cgggacgggg accgggaccg ggaccagcag caccggcgca ctggtctgcg gctcgatgcg
     6541 aaccgatgtg gcgccagccg gcacgaccac ggtttcgact tcgcgaatca ccccgccgcc
     6601 ggcgacactg ccgctggcgt tgccatagcc gatcacacgg gcttggcaag ccgcgcggtc
     6661 ttcacccgtg agcgcttcgc agcgcaggcg ggcgttggct tcgaactggg cgccgctgtt
     6721 gtcgagcttg ccgctgcgtt tttcggcagc ggcattgttc acttccctga gacaggtggc
     6781 ctggtcctgc tgggaccggc cctccataca ggcggcgcgc tccttctgcg tattgcccga
     6841 ggcgtcaatg cccgtggtgc cggtcgccgt gatgggcgcc tgcgccagcg cagcccccat
     6901 cagcagggac agccccaagc cggcccatgc gttgcgcgcg cgaaatgtgg ggttggtttt
     6961 gatcatgttg ttctcctgaa cggtgaaaga tgccccagtc taggaagacg gacgcacccg
     7021 acctgaggga gatcacgccc gccggacagc gccacgcacg cgactgtctg taggacgggt
     7081 ctttcagcgg ccgaaaaaaa aggcggccat ggccgcctgg acaggctctg gggggtagcc
     7141 cctgccaacg gattacctgg cgcgcgggcc gtcggctggc atggtgaacg agtcttccca
     7201 tgcggtcgcg aaacgacccg gcttgtcact ggtcgtgaag gacccggtgc catccgggtt
     7261 gaagccgaca gggcggctct tttcccgggg gtcggtgtcg gcagcctgtt tgtccttgag
     7321 ctccttgagg gcggcggcct tggcggcgcg caccttttca ctggtgaact cgtcaagcgc
     7381 cgcatgaaag ctgctgtcgc cgccacgggt ctcctcggct tcggcctccg acatcggaac
     7441 ctcgcccaaa aacgccgccc gcatggcacc gcccgcaggc agactgctgc tgcgcaccgt
     7501 ggtgtagccg atgccgcatt ccagcaacag gctttccttc atgtcctttt gcgccctggt
     7561 caggccccgt ttgcctggca catcgacgca gccgaccacc ttgccgtcca gcgtgcagac
     7621 agtgaagctg cagtaaacac cctccagcaa ctcgtgccag tgctggtcct tgttttcggg
     7681 ccggtttttg gtactcggaa tcgtgaagcg catgaccggc accttgatca tcaccgcatg
     7741 gtccgcaaaa gccgtacgca gccacttcca cacctcctgc tcctcgctgg tcatcaggcc
     7801 gcgggcattg agcagccagc gcgccggcaa ccgcagcttg cgctcgacgc ggcggcgtga
     7861 ccagctccaa tgcgccagaa tccccagacc gacgcccagc aaaaggccca gcgcggtcat
     7921 cagccatgtg gataggttca cggtatctcc ttcttgt
//
LOCUS       DAIDFG010000135         7941 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_135_length_7941_cov_15.508369, whole genome shotgun sequence.
ACCESSION   DAIDFG010000135 DAIDFG010000000
VERSION     DAIDFG010000135.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7941)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7941)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7941
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135_length_7941_cov_15.508369"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1416 a         2550 c         2534 g         1441 t
ORIGIN      
        1 gctgcgctgc gcgccgtgga tggcctgcag catggcgggg aagatatggt cgccattgag
       61 cagctcccgg acccggttgc cgcccaccag ggccgggccg agcagggcgc ccatggcgcg
      121 gcggaactgg gggtcctgcg tggcatacag gcgcggcaac tcgcggttga cctttttttc
      181 tccagtgctg aagttcagga cgagtagcac gccggccacg gtgatcagga aggtgagcag
      241 aatggtcagt gcaacgcggt tttttttcat catgggtcgg aaacggggtc gtggtacagg
      301 ggcgggtgcg acacgtgccg gtaagcgctg cgtcgatggt aaaaagcata agcagatgcc
      361 cggccggccc ctgaaagccg atgggctgtt gcgcggtagg ccgggaccct gtccggcccg
      421 gcgacaatag gcccatggca aaaaacatgc aattgcagga cattcttttc tcccagggct
      481 ttggcacacg gcgcgtctgc gccggcctgg ttcaacaggg ttttgtggcc gtggccggcg
      541 tcccctgtac ggagccagct atggattttg tagcggaagg gctgaagttc acggtgcaag
      601 gtgtggactg ggaataccac gaaaaggcct acctgatgct gcacaagccg gccggctacg
      661 agtgctcgca aaagcccagc acctacccga gcatctacac gctgctgccg gcgccgattc
      721 gccagcgcgg cggcggcgcg gctgccggtg tgcaggcggt ggggcggctg gaccaggaca
      781 ccaccggcct gctgctgctg tcggacgacg gcaagttcat ccaccgcatg agttcaccca
      841 agcaccatgt gcccaaggtg tacgaggtca ctgccaaaca cccggtcgat gacaaacagg
      901 tccagaagct gctggcgggc gtggtgctgg acgacgaccc caagccggtg cgggccgcag
      961 cctgcgaaaa aaccggcgag cagaccttgc gcctgacgct caccgagggc aagtaccatc
     1021 aggtcaaacg catgattgcc gcggtgggca accgggtgga agacgtggcg ggcctgcacc
     1081 gctcgcgcat cggttcgctg gatctgccgg gcgacctgaa acccggccag tggcgctggc
     1141 tcacggcgca ggacctggcg gccattgctg gctgagtacc agaaaccccg tgtctgatgg
     1201 agaaaattgc ctccagccct tatatgacgg gcgttaaaag ctattgaatc gatagcgtca
     1261 tggcagcggc tgcaggaaag cgacaaccgc ttccagcgtc cggtcgggtt ggtcgcgctg
     1321 cggcgaatgc ccgcaatcgg gcagccgcaa ctgcacggtc tgcggcgcgg ccaaggccag
     1381 ctcgtcgagc tgcaccaggg tgccgtattc gtcgcccagc ccctggatgg ccagcagcgg
     1441 cgcgctgatg tgccgacagt ccggccggat gtcaaagctg cgaaaaccgg gcgacagcca
     1501 gacgtcgttc cactgccaga aggcgctatc cacatcggca tgatggcggg ccagcttctc
     1561 ccgcaatcct cccgactcaa aggcagccgt tgcctggcca atcgcctgca cggcgatgtc
     1621 ctcgaccatc aggtgcggtg ccatcacgat gacggcgctc accgggtgcc ggctcgcatg
     1681 gagcagggcg atgctcgcgc catccgagtg gcccagcagc acgggcggct ggtggatgcc
     1741 ctggctggcg agcagggccg gcaacacctc ccaggcctcg cggtgcatgt agtcgggcgc
     1801 caggcggccg gtgccgcgca cgtcgggcac ggggtccgag cggccgtagc cgagccgcga
     1861 atacatcagg ccggcacggc ctgtggcctg gcagactgct tcgggccagt tgcggccgcg
     1921 ctgggtccac aggctgaccg agcccaggcc ttcgtgcagg aaaacaagcg gtgccaggct
     1981 ggccgaaccg gtgatgggct gcacttcaag ccgtgtgccg gcaatctcta ccaaattcat
     2041 caccagatcg taagggatgt aagcgtaccg agcctctggc gcgttgataa aagcctgccg
     2101 gtaaacttgc cggctgtccg aagaaagccg ctgcgtgaat ttccccttcc gtttgtttgc
     2161 agccgtcctg tgcacctgcc tgggcggagc cactgtcccg gccctggcgc agaccatgcc
     2221 cctgctggtg ctcaattcgc tcagtgacag cgtcagcgtc atcaatccgg tcgactggac
     2281 ggaaacccgg cgcattccca ccggcaagca gccgcaccac ctgtacctca cacctgacga
     2341 gaagtcggtg atcgtggcca acgccctgtc tgacacgctg actttcatcg acccccggac
     2401 cgccgacgtc cagcgtgtgg tgcgcgacat catcgacccc taccacctgc gcttttcgcc
     2461 cgacatgaag tggtttgtca cggcggccaa ccggctcaac cacatcgaca tctaccgctg
     2521 ggacggcacc gatatcgcac tggtcaagcg tatccccacc ggcaagacgc ccagccattt
     2581 gtggatagac agcaaaagcg ccacggtgta ttcgacgatg caagacagcg atgaactgat
     2641 cgcgatcgac ctgagcaccc agaccatcaa gtggcgcacg ccgacgggcc tgacgcccgc
     2701 cgacgtgttc ggcacggccg acgacaagtt cctgctggtc ggactgaccg gcagcgacgc
     2761 ggtcgaggtt tacgacgtga gcggcaagca gccgcagcac agccgcagca tcaagaccgg
     2821 cgccggtgcc catgcctttc gcgcactggg cgacggccgc catgtgctgg tcagcaaccg
     2881 cgtgggcaac agcatcagca agatcgacct gcagaccatg gccgtggtgg atgagtaccc
     2941 ggcacccggc gggcccgact gcatggatgt ctcgcgcgac ggaaaattca tttatgtgac
     3001 ctcgcgctgg gcgcgcaaac tcacggtgat cgacatggcc agccgcaagg tggtgcgcca
     3061 ggtcaatgtt ggaaagagcc ctcatggtgt ctggacgctg gatcacgcag cccggtgagc
     3121 ccccacgctc cgccgcttcg cgggtcgctg cccctcgagg gggcgctgcg cctgcgggcc
     3181 ggcaaagccg gacccgcggc cccggctggt ctggatacct ccctggggcg cccaacacgg
     3241 ttcggtctct tgtggcattc ctggttgcta catttttcat agctgttggc gcacgtccag
     3301 catgggctga cggctgtttg gatgctcaag gcagggccgg caagccggtt tacctgactt
     3361 tggacaccgg ccacatggag gtggcgccgc tgatcgcgga catcctcaac aaacaccagg
     3421 tcaaggtcac ttttttcgcc gccaacgagc gcacaaaaac cggggacggc agcctgggcg
     3481 cggcctgggc gccctggtgg aaggcgcgcg ccgccgaggg ccatgaattt gcctcccaca
     3541 cctgggacca cagctactgg cgcgccgacg tgcggccggc ggcgggcggg ccgacgcagt
     3601 tcaggatcaa accctcggcg ggcccctccg aaggccggga attcacctgg acggccgcgc
     3661 aatactgcga agaggtcggc cgtgccagcg cacggctgga ggccatcacc ggcaagaagc
     3721 cgctgccgct gttccgggca ccgggcggca agacctcgcc ggctttgctg gcggcggcca
     3781 aaacctgcgg ttatgagcat gtgggctggt cgcctgccgg ctttctgggt gacgaattac
     3841 ccagcggccc ttaccccaac gacctgctgc tgaaaaaggc attgcgcgat atccggcccg
     3901 gcgacatcct gctggcccat ctgggcatct ggtcgcgcca ggacccctgg gcacccgccg
     3961 tgctggagcc gctgatcgtc ggactggaag agcgcggctt ctgttttgcg acgctgcgcg
     4021 agcacccgcg ctaccaggcc tgggtggctg ccagtgggcc ggccatggcg cccgcggcaa
     4081 aatagcacca tggacagctt gatcgacggg gtgggcaacg gcttttctgc cgtgcagcaa
     4141 tggctgttcg aggccgtggt gcagccgacc ctgttcgcac tgggcctggc caactttctg
     4201 gaggacgcct tcctggggac ggcctggttc atggtcggcg ttatccagat actggtgctg
     4261 ctggcggtgt tcggcccgct gcagcgctgg cggccggtgg agccggtgcg ggaccacgcg
     4321 accgtccgca ccgacatgct gtacaccctg atccaccgtc tcggactgtt ccgcattgcg
     4381 ctcttcatga cgctggagcc tgtctttgac gaactgttcg gggtcctgcg cactgccggc
     4441 tatggcacct ttcacctgga ccagctctgg cccggcgtca ccgatggcgc ggtgaccagc
     4501 ttactgatct acctggtggc gtttgacttc ctgggctact ggatccaccg cggccagcac
     4561 cagctggata tctggtggcg cttgcactcg ctgcaccacg cgcagcgcca gatgacgatg
     4621 tggagcgaca accgcaatca cctgctcgac gacatcgcgg tcgactgcct gctggtgctg
     4681 gccgcgcagc tgatcggtgt ggcacccggc cagttcgtcg ccatcgtggc gctgacccag
     4741 ctcagcgaaa gcctgcagca tgccaatgtc cgcctctggt tcgggcgggt gggcgagcgc
     4801 ctgtgggtca gcccgcgctt ccaccggatg caccacagca taggttttgg ccatgagccg
     4861 gcggggaaaa acacggccag cggccacaac ttcggggtct tgctgccctg gtgggacgtg
     4921 cttttcggca ccgccaactt cgagcatcgt tacgacccga ccggcatacg cgaccaagtc
     4981 gagcaggcgc gcgactacgg ccacggtttc tggtcgcagc agtggctggg gctgaagcgg
     5041 ctggtcggca gggactgagc agccgtcgct ccggcgggtt atcctcaggc cctatgagca
     5101 agcttctcga cgccttctgg cgcgcaaccg tgtactgcct gcacccccgg gtgattgcgc
     5161 tgtcgttttt gccgctgatc atcatggtcg tcttctcgct cggggtgggc tacttcttct
     5221 gggaagacgc cctggccgcc gtgcgcagtg gcctggaaag ttatgagctg ctgagcacca
     5281 tggtgcgctg gctcgaaggc ctggggttgg gcaacctgcg ggtggtgctg gcgccggcgc
     5341 tgctgctttt catggcgatt cccgtgatcg tgattgcctc gctgctgttt gtcgcgatgc
     5401 tgatgacgcc ggccatggtg gcgctggtgg ccgagcgccg ctttccgctg ctggagcgga
     5461 aaaaaggcgg ctcgcttgtg gccagcctgt tctggtcgct cagctcgacc ctgctggccg
     5521 ccattgcgct gatcgtctcg atgccgctgt ggctcgttcc gccgctgata ctgatcctgc
     5581 cgccgctgat ctggggctgg ctgacctatc gcgtgatggc ttacgacgcg ctggtcgatc
     5641 atgcgagtgc cgaagaacgg cgcgaactct ttcgccgcca ccgcggcagt ctgctgggca
     5701 tcggcgtgct cagcggctac ctgggcgccg cgcccagcct gatctgggct tcgggggcga
     5761 tgtttgtcgc catggcgccc attctcgtgc cggtggcgat ctggatctac accctggtgt
     5821 ttgccttttc atccctctgg ttttcccact actgcctggc ggcgctggag caacttcgca
     5881 agcagaacaa tgccctggcc cccgaccccc ttgcgcagga tgctattgaa acgatagcgg
     5941 acgaacttct gcctcaaggc cccgcggggc ctgcgactac gctgggggcg ccgggtggcc
     6001 ggcccggttc cctgccatga ctttcctgtt tttcccatga ccacacactt cggactcatc
     6061 atcgtcggcg acgaaatcct gtcgggcaaa cgcgccgaca aacacctgcc caaggccatc
     6121 gagttgctgg gtgcgcgcgg cctgccgctt agttatgccg actacgtggg cgacgacccc
     6181 gaacgcatca cggccacgct ggcgcgcgcc ttcgacgccg cacgggcttc gggtgatgtg
     6241 gttttctgca ccggcggcat tggcgccacg ccggacgacc acacccgcca atgcgccgca
     6301 cgggcgctgg gcgtggaact ggctttgcat cccgaggccg agcggttgat ccgcgagcgc
     6361 atgcaggaca tcgccaggga gcagggcgtg ccctatgagc cggaccggca cgacaacatc
     6421 caccgcctga acatgggcat gtttccggcc ggcgccgcca tcattcccaa cccctacaac
     6481 aagatcccgg gtttcagtgt ccaagccggg gtgggcgccg tccacttcat gccggggttt
     6541 cccgtcatgg cctggccgat gatggagtgg ttgctggata cgtgctatgc cggcttgcac
     6601 caaaaattgg cttattcgga aaaatcggtg attgtgatgg gggccatgga ggcggcgctg
     6661 acaccgttga tgctggacat tgaggcccgc cacagcgggg tcaaagtgtt cagcctgccc
     6721 agcgtggacc accccgacta cgggcgccat atcgagctgg gcgtcaaggg ttcgccggat
     6781 gctgtcaatg aagcttaccc cgcgctgctc gcgggcttgc acacttttaa tgccaagttg
     6841 ggccccgaat tggtgcgata aatcactgca ccagtaaggt gcaaatctat gacgcacttt
     6901 attggtgctt cacttttctc gctgcttttg cgattttgcc ggtccggcgc cacccaaaca
     6961 ggggtcggat gacggcacaa tcaggggctt ggctttaaat caccggcaaa cacccgcgag
     7021 ggaggtttgg cacaaaaagt gcattcgacc acgccgaaac aattttttaa ccaccatcca
     7081 gcaacaggag attctgatgg caaagaccgt cgcagacgtc atgaaaatgg tcaaggaaaa
     7141 cgaagtcaag tttgttgact tccgttttac cgacacccgg ggcaaagaac agcacgtgac
     7201 cgtgcccgtg tcccactttg acgaagacaa attcacttcg ggccacgcct ttgacggctc
     7261 ttctgttgca ggctggaaag gcattgaagc ttccgacatg cagttgatgc ctgacccgaa
     7321 gacggccaac atcgaccctt tcttcgaaga aaccacgctg ttcatgagct gcgacgtgct
     7381 ggaacccagc gacggcaagg cctatgaccg cgatccgcgc tccatcgcac agcgcgccga
     7441 ggcctacctc aaggcgtccg gcctgggcga caccgcctat ttcggtcccg agcctgaatt
     7501 tttcatcttc gacgacgtgc gctggaacac cgacatgtcg ggcaccttct tcaagattga
     7561 agagtacgaa gccccctgga attcaggcgc caagctcgaa ggcggcaacc gcggccaccg
     7621 ccccaccgtc aagggtggtt acttccccgt gcctccggtg gacagcacgc aggacatgcg
     7681 cgccgagatg tccctgattc tcgaatcgct gggcattccg gtcgaggtgt tccaccacga
     7741 agtggccggc gccggccaga acgaaatcgg caccaagttc agcacgctgg tgcagcgcgc
     7801 cgactggacg cagatcctca agtacgtggt gcagaacgtg gccaatgcct acggcaaaac
     7861 cgccaccttc atgcccaagc ccatcgttgg cgacaacggc tccggcatgc acgtgcacca
     7921 gtccatctgg aaagacggca a
//
LOCUS       DAIDFG010000136         7890 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_136_length_7890_cov_16.451053, whole genome shotgun sequence.
ACCESSION   DAIDFG010000136 DAIDFG010000000
VERSION     DAIDFG010000136.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7890)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136_length_7890_cov_16.451053"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1391 a         2485 c         2680 g         1334 t
ORIGIN      
        1 ggcacgtcta tcccgacgcc acgcatgcct gggacaaaag caacgcgagg cgcggttatg
       61 tgagaagcag ctcagtcacc gccgacgcca tgcgacgcac gctcgacttc ttgaaagcga
      121 actagaaacg ttggcgcggc ttgagggcgg ggattgcagt gggccttgaa acagcctggt
      181 ctggctacaa tgctggttat ttatacagcc cggcattcac cacgcctttt ccccatgagc
      241 actcccggca accaggacga caagcgctat gttgtcgccg tgcgtgcgct gtgtgaattc
      301 acggccaaac tcggcgacct cgatgtgcgc ttcacgccct cgccgtcggc cctcgaaggc
      361 atggcgggcc accgcatggt ggcgatgcgc cggccggcgc attaccagac cgaagtcagc
      421 ctgagcggcg tctacaaaga cttgctggtg cgcggccgcg ctgacggcta cgaccccgaa
      481 ctcggccagc tcgaagaggt caagaccttc cgcggcgatc tggaggccat gcgcgccaac
      541 caccggcatc tgcactgggc gcagctcaag gtctacggtc acctgctctg ccagaaggaa
      601 ggcttggccc aggtcaagct ggcgctggtg tatttcaaca tcgccacgca ggatgaaacc
      661 ctgctgaccg aagccttcag cgccgccgag ctggaagttt tttttgaaga acagtgcgac
      721 cattttctcg actgggcccg ccaggagatg gcccaccgcg ccgcgcgcaa cgaagcgctg
      781 cagaccctgg tttttccgca cgagagtttt cgccccggcc agcgcgattt ggccgaagcg
      841 gtctacaagt cggccagcag cgggcgctgc ctgatggcgc aggcgcccac cggcatcggc
      901 aaaaccgtgg gcacgctgtt tccgctgctc aaggcctgtc cgacgcagca gctcgacaag
      961 gtgtttttcc ttgcggccaa gacgccgggc cgccagctgg cgctggacgc cgtggcccgc
     1021 atccagcgca gcgcgccggc gctgccgctg cgtgtgctgg aactggtggc caagggcaag
     1081 gcctgcgaac acccggacaa ggcctgccac ggcgacgcct gcccgctggc caagggcttt
     1141 tacgaccgcc tgccggccgc gcggcgtgct gccgtggctg tggtcggccc gctcgacaag
     1201 ggcgcactgc gcgaggtggc gctggcgcac ggggtctgcc cgtattacct cggccaggac
     1261 ctgctgcact ggagcgatgt ggtcatcggc gactacaact actacttcga catcaacgcc
     1321 atgctgtacg cgcagacgct ggccaaccag tggcgcgtca gcgtgctggt cgacgaggcg
     1381 cacaacctgg tcgagcgcgc gcgcaagatg tattcggccg agctggaccc ggcacgcctc
     1441 gcgttggcgc ggcagggtgc gccggtggcc atcaaacgtg tgctggaccg cctgcagcgc
     1501 agctggtacg agctgcacca ggaccaggcc gaggcctacc aggtgtacga cgaggtgccg
     1561 caggcctttc gcggcgcgct gcagcagacg gccagcgcca tcctggacca cctggccgac
     1621 catccggccg gcatagacgg caggctgcag gactttttct tcgaggtgct gcatttctcg
     1681 cgcatggccg agctgctcga tacccattcg ctgttcgaca tcaccgacgc tgcgggcagt
     1741 gggggcggtg ccgtcttgtg cctgcgcaac gtcgtgccgg cgggttttct gtcgcagagg
     1801 tttgcggcgg cacgttcggc ggcgctgttc tcggccaccc tgagcccggc gcactactac
     1861 agcgacatgc tgggcctgcc cggggacacg gcctggattg atgtggcctc gccgttccgg
     1921 gccgagcagt tgtcggtgca ggtggtggac gacgtgtcca cgcgtttcca gcatcgcggg
     1981 cattcgctgt cacccatggt cgacctgatg gcccggcaat acgcggcggc gcccggcaac
     2041 tacctgagtt ttgtcagcag cttcgactac ctgcagcaac tggccgagtt gttcaaggcg
     2101 cgttacccgg ccattccggt ctgggagcag tcgcgcggca tggaggaaag cgccaagcgc
     2161 ggctttctcg aacgcttcac gaccgagacg catggcatcg ggtttgccgt gctcggcggc
     2221 gcgtttgccg agggcatcga cctgccgggc aaacgcctga tcggcgcctt cattgccacg
     2281 ctggggctgc cgcagatcaa ccccgtgacg gagcagatcc gccagcgcat ggaggccagt
     2341 ttcggcaagg gttacgacta cacctacctc taccccggcc tgcagaaggt ggtgcaggcc
     2401 gcgggccgcg tgatccgcac cgagtccgac agcggcgtgg tttacctgat tgacgaccgt
     2461 tatgcgcagg cgcaggtgcg cgagctgttt ccgggctggt ggaaggtcgg ctaccagcaa
     2521 gcgcgtttgc cgccgcagcg cgccgcgtga gtcggctgct caaaaaattt caataaaaaa
     2581 tgcctgtagc ccagcagggg cgggcgcagc cagctatgaa atgtatagcg gcgatcagcc
     2641 gaacaagcca tgccggcgcg ctgccagcat ggcctgcatg cgtgtggaca cccggagcag
     2701 gccgtacagg atctggacat gcccgcgcac ggtatggtgg gacaggttca gctggcgcgc
     2761 gatcagcttg tcggacaggc cttcacacag gagggccagc acctggcatt ggcgcgccgt
     2821 caggggcgcg gcgagcatgt ggtcagtgcc cgcggccggc gcggcggccg ggctgtctgc
     2881 ctggcacggg ggctgttcat cggaggcgag cgtttgcaaa gaggacatgc ctgaattaaa
     2941 tcggcagcga cgcggcgcgg caagtgccgc aggcgccccg ggccggaaat gttgtcacca
     3001 gaattggtga cagacagaca aggcaaacgg gcggcgctca ggctggcctg cgtttttcgg
     3061 gcagccacaa gttcaggcgc gtgcccggtc cctggccgtc ccagcgcagt tcggcaccga
     3121 tggatcgggc ccggcgcaac tggttgtgca ggcccatgcc gccttttttc aagccgcttt
     3181 ccagcgaaaa ccccgggccg ttgtcggtga tcgtgactgt cacgccaccg ttgccggaac
     3241 cggtggcgac acggatctcg ctggcttgcg tgtgtttgag gatgttggca aaagcctcct
     3301 gcaggatacg caggatgtgc agcgcgttgc gcgggtccag ccagtccagc ggcggcacgt
     3361 ccaccacctc ccagcgcagc gtgatgcccg cgctggccag ccgcggaccc aggcgaaagc
     3421 gcagcgttgc cagtagcagc agcaagtcgg cctcgaccgg ctccatcgag tcgatggtga
     3481 gtttgaggtc gtcgatgcag cccttgagca cttccgccac ttccgcctcg ctgacccgcc
     3541 cgccttccac cacacgcagc gcgctgacca gggaggaacc caggccgtcg tgcatgtcct
     3601 gcgtcaggcg ctggcgctcc tggctcagcg tctggcgttg ctcggcctcg cgcaggcgct
     3661 cgtagctggc cgccagctcc gtctcgcgcg cctgcaggcg ctgctccagt tgggcgttgt
     3721 ttttctccac gtcggcaatc gcgcccacgt agcggcggtt catcaggtac ccgaagacgc
     3781 tgaaggtgat cgcgttggtg taggcgctca ggtaccagcc ttccgggctg atgaagttgt
     3841 tttgcagcag ccagtccacc gcgcccagcg cagtgcacac gccgatgccg acggccacca
     3901 gccgtccctc ggcagagctg cgccagctct tgatgcctcc cgcgaccgcg acagtggtgc
     3961 tcatcagcat cgcaaacgca taaatcagcg gcgtgacctt gggcgtgttc ggcagggtgg
     4021 ccagcgcggg cagcgtcagc agcccgatca ggctggtcga caccaccagg gcgcgggtca
     4081 gccagatcag cggccggcca tgcaactggc ccagcgaaaa gtgcaccacc gtgatcaacc
     4141 acaacaggga gttgaccgtc agccaggcaa accagtcgtt ggccacggga aggtccacat
     4201 aaaaatgcag gctccgcgcg aaggaggtca ccgccaggac aaaaaagagc aggtagccgg
     4261 tcgcctgcct gcgccgcagc cagacaaaaa aagcaaacac accgatcgcc aggaaggcgg
     4321 cgctgaacag cgccggcaga tccagctgca gccacttgcg caagtggtgg cgcccgctca
     4381 gggcctcggc cgggcccagc cacagcgacg acagcgcgac ttgggtggcg cgtgtgtgtt
     4441 ccagccggac caggatctcg cgcggtggct tgccggagcc ttcgccttca agaaggaccc
     4501 aaagcggggt gcgcgtgctg tcccacagcg ggccctgaag ctgtgcccgg tgcaccagct
     4561 gtccgtcggc atacaccgcg acggtgccgt tggtcttgac gcgtgcaccg tagagcgcca
     4621 tggggccggc gccggcggcc agcgcgggca cactcacgcg gtaccaggtg atgcgcgcgg
     4681 tgccggccgg agcaatggcc gaggcggcct ggcgcagcag ggccaccggc agggcatggg
     4741 gaagttcaac cggctgccag gcaccgggca gggcggcgct gtcgagcgtg ggcggtggca
     4801 ccgtgaaccc gggggtgtcc agggcctgcc actgcgcttc ggtgatatgc agggtgcttt
     4861 ccgggccggc ttccttcgca ccggcggcgg tgcaggcgga tgcggccagg aagaagccaa
     4921 gcagcaggcc cgcggcagca cggagaacgg taaaaaaaag catgggggca gttatagcag
     4981 ccctgccggg cgaaacctga accgccaggc cggcactcag gcggccagga tgccctgctg
     5041 cctggcctcg tagatcgctt ccgccttgga ggtgaccttg agcttgccgt acacgcgtcg
     5101 cacataggtc tgcacggtgt gcaccgagac ctgcatgagc cgggcaattt cctcggcggt
     5161 aaaccccttg gtgatcagtt ccagcacttc cttttcgcgg ctggacagct gcacctgggc
     5221 gttgccggtc ggcgcaggcg gtgcggccgg cgccgggaca gaggcctggc gaaaacgcgt
     5281 caggatctgc cgcgcaatca gcgggctgat cgggctgccg ccgccgtgca ggcttcggat
     5341 ctcggccagc atgttgtccg gtgcgctgtc cttgagcagg tagcccgagg cgccggcctc
     5401 gatggattgc agcacatggg cctcgtcgcc aaaggtggtg cagaccatca cggcgcaaag
     5461 cggccactgg cggtgcgccg cctggatcac gtcgatgccc gagccgtccg gcaggcccag
     5521 gtccaccagc agcacgtcgg cggctgcggt ttccagcgcc agcagtcctt cggaacgggt
     5581 actggacacg ctgaccaccg tcatgtcggg cgcagcattg atcgcggtga ggatggcgcc
     5641 ctggaagtgc acatcgtctt ccacgagggc cacgtgaata agcggtgtgc tggcgtcaga
     5701 gggcatgcgt ggatgggggg gcagggatgg ggcgcagggc ggaaggctgc cattttgcgc
     5761 caggatggtc cggcgcgtgt cactaaaact agtgacgccc ggcctggcaa gaccggcgca
     5821 ccggcgggat gccgggatgt gatggattca atggctgccg gcgcagcaag gatgagggcc
     5881 gggggccgcc cgccctactg cgcctgaaat ccgctggcct tgatgatgcg tgtccaggtc
     5941 tgcgtgtaag cgtgttcacg cgttgcgagt tgctgcggcg gcatgtaacc cacgctcagg
     6001 cccatggctg tgagcctgtc gcgcacgtcg ggcatggcga tgaccttggt cacggctgcc
     6061 gcaaagcggt cgatggtggc ccggggtgtg ccggccggcg cgaagatgcc gtagtagggc
     6121 acgtcttcaa agccggccag gcccagctcg gcaaaggtcg gcacgtcggg cagggcagcc
     6181 tggcgcttgc ctccgagcac ggccaccacg cggatcttgc cggccttgtg gttctcgatg
     6241 aaatcgggca ccgagccgac gccggcacca atctggttgc ccagcatgtc ggccatcatg
     6301 ggggcgctgc cccggtaagg cgcggcctgc aggtccagcg catacttctg gccgaccagt
     6361 ttgaccagga actcgggcac cgaggcgggt gcgggcacac ccacgttgcc cttgccgccc
     6421 tgcgtgcgca cccaggccac gtattcgttc atggaggcgg cgggggtgcc gcccgacacg
     6481 gcgaaggcat tcacaaacgt ggcgaatccg gccaccgcga caaaatcctt cgccgggtca
     6541 aagccggggt ttctggtcac cagcggcagg atggaaatgg tgtggtcgtg cgagaggaaa
     6601 aaagtcgtgc cgtccgccgg tgccgccttc agggcctggg ccgcaatctg tccgcccgca
     6661 cccgcgcggt tctcgacaat gacgttgctg ccgagctggt ccttgagctt gtcggccagc
     6721 gtgcgggcaa tcacgtcggt gccgccgccg gccggaaaac cgaccagcag ccggatggtg
     6781 ccggtttgag cctgcgcgat acccgcgagc agaagggcgc cgacggcgac ggtattgcgc
     6841 agcgtgtaca ggacacgccg gcttgttgaa ctgaccatgg aagactccgg ttcgaaggta
     6901 aaaacaaagg tgggtattct gacgcaacaa cggtgccagc cgcgggctgg gaccgggcgc
     6961 ggttccgggg gcggccgcaa gcggcgatgc ggccgtcagg ccgtcaggtc acgaaggtgc
     7021 gcacgaaatc gagtgacgac acgacacccc ggatgtcatt gccctcggcc accaccacgt
     7081 ggtggtggct gaagttgacc attcgcgcgg cccggtatgt ccccgcggcg gttgtccatg
     7141 cgccagacct ggtgggcgtg acttgtcatg cattgaattt cccccagttc agtccagccc
     7201 gcgttcgcgc ctggagccca gccttcgtgc catgccggcc ccgccatggc agtgaggatc
     7261 tgagttaggt cagtaaatac ccatttagtg acgaaacgcg cagttacgcc ggcaatgagc
     7321 cgataaagcg cacatggaat tggttagact gaaagtgtta atttgtaaca aaatgtggta
     7381 attttttgtc ggggaaaatg ggatacaaat cttatgaagc tcagcggctg caatgcttcg
     7441 cttggcggga ctgtcaagac gttcgttgca gattttgatg cccgcccatt actgcccgga
     7501 aaatgcggca actgtgcctc gtatgagttt tgcatcgcgc gcggtctgtc cctcaaaaat
     7561 tcagtcgatg gcggaggagt actgatcgaa gaacacagct tcaggaaggg cgacatatta
     7621 gcgcgggaag gcgagcatgt gcatcacctg agggtcgtca aggtcgggag cgtgtgtctg
     7681 ggccgcaggg gctccgatgg acatatcgat ccagtcgcca ttttgggacg cggcgcagtc
     7741 gttggttttt gcagttactt cgggcaaccg aaccaacagg gaagcgttgc cctgagcagc
     7801 ggtcggtatt gtgaaatatc aacggaggcg ctggcctgtc tggcgcgcag tgcccagaag
     7861 atctgggatc aggtcggccg agcctgcagc
//
LOCUS       DAIDFG010000137         7838 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_137_length_7838_cov_17.557754, whole genome shotgun sequence.
ACCESSION   DAIDFG010000137 DAIDFG010000000
VERSION     DAIDFG010000137.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7838)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7838)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7838
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137_length_7838_cov_17.557754"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1384 a         2417 c         2548 g         1489 t
ORIGIN      
        1 cgcctgctgg aagactccca ccgcgaggtg gacgaccaga cccgccagag cgccattttt
       61 tcccgtaccg aggcactcgt gatggagagc gccgccatgg gccgtgcgcg gggcgcgacc
      121 ctggtggatc tgcaggacgt cgaggcggcg cttcgtgccc gcaactacag gcacgattac
      181 cccgagcagc gccagcagga aaccattgcg gacggggagc ggctgctggt cctgaccggc
      241 gagaaggtgg gtcagctcaa tgccctgacc gtgattgacc tgggcgacca tcgtttcggt
      301 ttcccggtgg gtgtcacggc gcgcacctac gccggcgacg agggcctgct caacgtggag
      361 cgcgaggtgg agctttccgg ccccatccat gacaaggggg tgctgatcct gcacagttac
      421 ctgtcggcgc tgtttgccca cattgcgccg ctggcgctga atgcggccat tgtttttgaa
      481 caggagtacc acggcgtcga gggcgattcc gcgtcctgcg ccgaactgta tgtgctgctg
      541 tcgtcgctgt ccgggttgcc gctgaaacag ggcgtcgccg tgaccggggc gatcaaccag
      601 catggcgaca tgctgccggt gggcgggatc aacgaaaaaa ttgaaggtta tttccgcagc
      661 tgcgaaattg tcggcctgga cggcagccag ggcgtgctga ttccgcaccg caaccggcgc
      721 cacctgatgc tcgagcgcaa ggtggtggag gcggtggccg cgggacggtt tcacatctac
      781 acggccgaac atgccagtga aggcctggaa ctgctcaccg gcacgccttt tggcgagttc
      841 ggcgcgtcgg gcgactaccc ggaaggcagc gtgctgggcc gcgcgcagaa aaccctgcag
      901 gatttccggc gctcctgcca gcgtgcggag cagcacaagc ccgaacgtaa acccggaaaa
      961 ctgcgctgac cgggttgcga cccttgtttc cctatttttg tttcggcatc cggcccatca
     1021 ggtaaaactc ggggttgggc atcatgctgg aaaagctcgc gatacggttg gacaggccga
     1081 agaaggcggt gatcgcggcg atgtcccaga tgtcctcatc gttgaaaccg tgggcatgca
     1141 gcgctgcaaa gtcggcttcc tcgatctcgt cggatttttg gcagaccttc atggcgaagt
     1201 ccagcatggc gcgctggcgc ggcgtgatgt cggccttgcg gtagttcacc gccacctggt
     1261 cggccaccag cggttttttc tcatagattc gcagaagggc gccatgcgcc accacgcaat
     1321 acaggcagtg gttggccgca ctcgtggtgg tcacgatcat ctcgcggtcg cctttgctca
     1381 ggcccgactc ttccctcagc atcagcgcgt cgtggtaggc gaagaaggcg cgccattcgg
     1441 ccggacggcg tgccagcgcg aggaacacgt tgggcacgaa gccggctttt tcctgcacct
     1501 ccagcacttt ggccttgatg tcatcgggca gggtgttgag gtcggggagg gggtatctcg
     1561 tggtcatggc ttgtctcatg gcgggtgaat gttggggcaa tgatacgttc agagtgaggg
     1621 gattttgggg tcggccctgt cggcgcagct tgttaggagc gtcagcggct atgctggtga
     1681 tttttccagg agcacctgac ccatggattc cccattcgac aaaggtctcg ccacccgcaa
     1741 gcaggtgatg ggcgaagact tcgtaagcaa tgctttcgcc aatgtcaccg atttcacccg
     1801 tcccattcag gaacacatca ccgccaaagc ctggggggat gtctggcagc ggccgggcct
     1861 ggagcgcaag acgcgaagcc tgatcaccgt ggccatgctc acggcgctgg gcaagcagca
     1921 tgaactcaag gcgcatgtgc gcggcgcact caacaacggc gcaacttctg cggagattca
     1981 ggaggtgttg ctgcacgccg ctgtgtattg cggtgtgccc acggcggtgg atgccttccg
     2041 caccgctgcc gaggtgattg acggcaagta ggacgggaaa ccgccggccg gccggcatgg
     2101 ctggtttttc tgcgcgtggg ctcgacttga catgatctgc cgcaaagttc ctgttgcgcc
     2161 ggctacccat aatggttggc atgatgtggt tcattcatgc catccagcgg gtcgcccgcc
     2221 atgcgattgt gttgtcgctt tcgctggggc tgttcatgcc tgtctggggc gccggcggct
     2281 cccaggcgct cgggcccgcc gctttgcggg ccaagcatct tgccttgggc cctcaacttg
     2341 ccagcaatca gttccagggg cccttgtacc tggcgtcgac tgaaagcgac cagcagctgg
     2401 agggggatgt ttacgcccta gtcaagcacc cgttcggaac ggtcagtgcc gaactgagtg
     2461 accccgggca ctggtgcgac gtgctgatcc tgcatctgaa catcaagttc tgccgtgcca
     2521 ggactgccgc gggggctccc cagctggatg tgcggatcgg tcgcaagcat gaacggcccc
     2581 tggttgaggc gtccacggtt cttttttcgt accgcagggt ggccgtagag gcggactatc
     2641 tggatatcga gcttgaagct gctgatggtc cgctggacac acacgactac cggattctgc
     2701 tggaggccat cccgatttcg gaagggcaaa gcttcattca tatgcggtac tcgttcgcct
     2761 atgggcgcgc gggtcgcgtc gccatgcagc tttatctggc aacccttggc cggggaaagg
     2821 tcgggttcac cccgaccaca gcgggcattc ccggtcagcc tccagagctc atcggtggtg
     2881 tgcgcggtgt ggtggagcga aacacgatgc gctactacct tgcgattgag acctacctcg
     2941 atgcgctttc ggtgccgccg tcgcagcgct tcgagaaagg cctggagggc tggtttgcgg
     3001 cgacccagaa atatgcccgc cagttgcatg aggttgaccg tgccgcctac ctcgacatga
     3061 aacgcaagga gtaccagcgg cagcagacgg caccctagta ctgcagcccg aaatatcgaa
     3121 acatgaaagc gcgccgtcgc acccacggcg gcgttgccat cctggcgaag gcgtacagcc
     3181 ttcactgcga ctggcgtctt gccctgggca cgaagtcatc actttcattt tgttccgcta
     3241 cttccgtgct gcagtaccag gcggagagcc gcatgccccg cagcgggtgg ttctggttca
     3301 tgctgtccag ttgataaatc tcaagttctg aatatcggta gcgtgaaata cttaattctc
     3361 tcgttcatca accctcctgg agatttctca tgaaaaaact gttgcttccc ctgagtcttg
     3421 ttcttgccca gatgttggcc gtgagtgcct tcgcccagac caaagggggc gaagccgacc
     3481 ctgaacaatc caagggccag accctgccca aggcgacgcc tgctgaaaaa gccactgcca
     3541 aggctggccg caaagcggaa ggcgccgctg tcgccggcca gggcgtcaag ccgcttgcag
     3601 cccaggaaac tggtgccggc accgccaagg cggccaccaa gcaggagcgc gatgcggccc
     3661 gggccaagcg caaagccacg gccgctgccg ctctcaagaa gggcgagatc ccttccgggg
     3721 aaaaatgatc cagcctggct ggtcagcgaa tgacgggccc ttcggggtcc gttttttttg
     3781 cggtgatgcc ttcaaaggtt tgcgtccgat tttccgcatt gcctgcgggc agaaatatcg
     3841 cctgcagccg gcgccgtgaa acgcccggat ttcagtctcc ctactccggc gggggaatgc
     3901 ggactacaca ccatgaggcg ggcagggcgc acaatgaatt ttcagtcgcc gccgtgtgac
     3961 gtcatggcaa cccggccttc acgccgcgtg ttgcagaccc tgaaaggaaa aatacatgca
     4021 agttcaagtc aactccaacc acaccattca caccggcgag tcatttgagc ggtgggcgag
     4081 cacggaactc aacgagtcgc tgagccgctt caaggatgac attacccgca tcgaggtgca
     4141 catgagcgac gagaacagcg acaaggtcag caccgaccac aagcgctgca tgatcgaggc
     4201 acggctcgca caccatgagc cgctggccgt caaccaccac gcctccagcc aggatgaagc
     4261 cttccggggt gcgagcgaga agctcaagcg ggtgctcgaa cacacgctgg acaagctgcg
     4321 cgaccaccgt tcacgggagt ccatccgccg cgagcccgat ccgggcgctc tctgagcctg
     4381 cagccagcgc cggctgccgt cgcatttccg gggagcacct tctgttttaa agtgtttttg
     4441 gcttccagcc caatgaatac aagcgcggat agctattgat atgatagcaa tctggtcggt
     4501 aggtgttgcc gccctcctgt gcgcgggagg ctcaacgccg gccggttccc gattgcaagc
     4561 ccgggatcag tttcaagggc gatgatgctt accacctcga actgaccgac cgatgaaaat
     4621 tgccctgctg tccgacattc atgccaacct gcgtgcgttt gaggcctgca tggaccacgc
     4681 cgccgcccag ggcgcgcagc gtttcgccgt gctcggcgat ctggtgggtt acggagcaca
     4741 gcctgcgcag gtggtgcggc gggtgcgagc gctggcgcag gatggcgcca tcgtccttca
     4801 gggcaaccac gacgcgttgg cagtgactcc gcagggccag gccggtacga tggacgccgg
     4861 tggtgcggcg tggacccgct cgcaactgga gccctctgac cttgatttcc tcgccggcct
     4921 gccgctgacc gccaccgacg acgccatgtt tctggtgcat gccagcgccg acaagccgca
     4981 gcgctggctg tatgtcgaca gcgggccgcg ggcggaggcc agcctccatg cggcctgcac
     5041 ggacacgcgt gtgcgttatg tttttggcgg ccacgtgcac cagcaggccc tgtatttccg
     5101 gggcgcgagc cagaagctcc tgcgttttgc gccgacatcg ggcgccacca ttccggtacc
     5161 gccccatcgc cgctggattg ccaccgtggg ctcggtcgga cagccgcgtg atggcgacac
     5221 cgacgcgatg tatgcgattt ttgatgccgt ccgcgctgaa ctgacctttc accgggtcgc
     5281 ctatgaccac ctcgcggcgg cggccgatgt ccgggcggcg gggctgccgg atgtttttgc
     5341 gaaacggctg gagcagggct tgtgaagctg ctggaccccg ggacgttgat tgacggcttt
     5401 caggtaggcg actgcctgca tgccggtggc atggcccaca tctacgaggt tgcttatgca
     5461 ggcgctgcgc agcgcgagcc ctttcccatg gtcatgaagg tgccgcgcat gaccgagggc
     5521 gacggtgcgg aaaacatcgt cggtttcgag gtggagcggc agatcctgtc cgtgctcacc
     5581 gggccgcacg tgccgcgcct ggtggcggcc ggcgacctgg cgaagctgcc ctacctcgtc
     5641 atggaacacg tgcccgggcg taccctgcaa caatggctgg acgatgatgc cgcaccgctg
     5701 ccggctgaag agatcgcccg gctgggcgcg gccgtggcgc aggccgcgca cagcctgcat
     5761 ctgcaggacg tctgccacct ggatctcaag ccggccaatg tgctgatccg gccggacggc
     5821 ctggcggtgc tgctcgactt cggcctgtcc tgccatgccc actacccgga cctgctggcc
     5881 gaggaattac gccccgctgt gggatcgccc acctggatgg caccggaaca ggtggtgggc
     5941 atgcgcggcg acccgcgcag cgacattttt gccatcggtg tcatgctgta cgagctggcc
     6001 acgcgtgagc ttccttttgg caacccgcaa accaccggcg gactgcgcca gcgcctgtgg
     6061 atggacccgg tgccgccgcg caagtggcgc ccggccctcc ccgaatggct gcaggaaatg
     6121 atcctgcgct gcctggaacc ggaggccgcg cggcgttatg cgtccgccgc gcacctggcc
     6181 ttcgacctgt cccatccgca gcaggtgcgg gtgaccgacc gcgggcgccg cgtgcggtcc
     6241 accgggttct gggcgcatct gaaacgctgg gtgcgcgccg ccggcatgca ctaccagccg
     6301 agcgcgctgc cgtcacggca gattgacgag gtgcccatca tcatggtggc cgttcccaac
     6361 cgcgacgtga gtgacgccac cctgtactcc ctgcgcgggg ccgtgtcgcg tgccctcggt
     6421 accgagcccg gtgcacggct ggcctgcgtg agtgtcatca gcccgggtga aacgccgggc
     6481 cacgacgccg cctccagcga gaccacggtg caccggcgct acctcggcat gctgcggcaa
     6541 tgggccgagc cgctggacct gagcggccag acggtctcct tccacatcct cgaaagtggt
     6601 gacgttgccc aggccctgct gcgttatgca caaggcaacc atgtcagcat gatcgtgatg
     6661 ggcgcggcca cccacgggct gcagatgcag cggctgctgg ccacggtacc cgtccgggtc
     6721 gccatggcgg cgccgtgcac cgtgatgctg gtcaagcagg ccctgccgtt cgaccagctg
     6781 gccgccacgg gcggccccga acaggataat ctgggcgaaa ggcccgcacg tgtttccagg
     6841 gtgatgatct aggaagtcgc cagtcctttg caggaaagcg gaagctgcta tcaataaaag
     6901 agcgtccggc tcaacccgcc atcagcttgt gcaccaggtc ggcggtcgtc gaggccttgt
     6961 acttgcgcat caggcgggcc cggtagatct ccaccgtgcg gtggctgatg accagggcgc
     7021 ggccgatttc cttggaggtc aggccgtcca tcaggtgcgc ggcgacctcg cgttcgcgcg
     7081 cggtgagttc ggccttgacc ggccggcgtg agctgaggtc ttcgaaggtc cagatgccgg
     7141 actcgtgcgg cgatgcccgg ttcagcgcgc gccccgtcac atggcaccag aaggtctcgc
     7201 ccttgaggcg gccgtccacc cgtttcatga tgcggtcgtc ggcgtagtgc cccttggcgt
     7261 tgagaatggg cagcatgcgc gcgcctatgc gctcatattc gtccgcgctc gggtaaagga
     7321 tctggaacga ctggccggtg agctgctcgc gcgtggcccc gaacatctcg cacagatgct
     7381 ggttgcagtc gacaatggcc cgattgcgcg aaagcaccag gcccacgggg gccatgtcga
     7441 atgccaggcg gtagtcaatg tccatggagg tgcaggtgag ccgggcgtgc gggtttgact
     7501 ttagggataa ctacgtatgt ggataggtag tatcgtaatg cttttaggtt cgtgtcgaag
     7561 ctctgcagga gatttctgtg aataaaattt tcccctccgc tgatgaggcc ctgaagggcg
     7621 tcgtccagga cggtcagctg atggctgtcg gtggttttgg cctgtgcggc attcccgagg
     7681 ccctgatcga agcgctcaaa gccagccagg tcaaaaatct gaccgtgatc tccaacaatg
     7741 ccggtgtgga tggcttcggt ctgggcaagc tgctcgagac acggcagatc aaaaaaatga
     7801 tttccagcta tgtgggcgag aacaaggagt tcgagcgc
//
LOCUS       DAIDFG010000138         7830 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_138_length_7830_cov_16.268424, whole genome shotgun sequence.
ACCESSION   DAIDFG010000138 DAIDFG010000000
VERSION     DAIDFG010000138.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7830)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7830
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138_length_7830_cov_16.268424"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1323 a         2476 c         2563 g         1468 t
ORIGIN      
        1 tggaacaacc ttctgacacc ccgcccgacg gcgactttgc ccggtatgtg gaaaaagtga
       61 gtgccaccgc ggcggcggct gtccttgccc ggcaggcgaa ggctgacgcg cagccgggca
      121 ccggcctgat gccaggggcc gccacgccgg gacgccagga ggccgccggg gaggcagcgt
      181 cgagccggcc cgccggaccg tccttgtgga cagtgggcaa gtgggtgctg ctcgcctgga
      241 tcgcgctgca gttgctctca gtcctggttc cgcgcgctgg catgttcgcg gtggccatgc
      301 cgctcgcttt cgccgcctgg gccatttacc gttttaaaaa atactctccg gaactgctca
      361 aggcgcgctt gcgtgcgttg gctgaacagg ccgccaaaga attcaaacaa ggcaagtgac
      421 atgaaaatcg acgtgaagat catcgacgag cgcctgcgcg ggaacctgcc cgcctatgcc
      481 acgcccggca gcgccgggct ggacctgcgc gcctgcctgg acgcgccgct gaccctgcag
      541 cccaatgcct ggcaactggt gcccaccggc ctggcgatct atctgcacaa ccccggttac
      601 gcggccatga tcctgccgcg ctctgggctg gggcacaaac acggcatcgt cctgggcaac
      661 ctggtcgggc tgatcgacag cgactatcag ggccagctca tggtcagcgc ctggaaccgc
      721 agcgacgtgg ccttcacgat agaacccatg gagcgtatcg cccagctggt gatcgtgccc
      781 gtggtgcagg ccgagttcca tgtggtcgat gatttcgcgg cggccagcca gcgcggtgaa
      841 ggcggctacg gctcgaccgg caaggcgtga gcaaagacgc cgcagcgtca ggcttttcct
      901 acacctgccg gcagtgggct cccacgccgg aacagcctgg gaccccacct gtccgacggc
      961 gcggtgtagg tgtaatcaat gcagcagggc cctcaggcaa cttgtcttca acggcatgga
     1021 tgcagcggac cctctgaacg ttcacacaca ggagatttcc atgcgtcaag cttcccgttt
     1081 catttcagtc gtttcatccg tggttgtggt cgccgggctt gccgcgtgcg cggcgcccat
     1141 gagccagccg gcgcagggct cgacctaccc tgcggccacc tatcccgcct ccacttaccc
     1201 tgcgcagaac ccgcagggca attacatcga atacggccgc gtcagcaatg tggaagtcct
     1261 gcgtacgcaa caaccggcta caacctctgg cctgggcgcc gtgatcggcg gcgtggccgg
     1321 tgcagccgtg ggcagccaga tcggcggcgg tagcggacgc acggcagcca ccgtgatcgg
     1381 cgctgtcggt ggtgccgttg ccggcaatgc cgtggagaag aaccgcaaca cccagcccgt
     1441 gaatgaaagc taccgcgtgt ctgtccagct ggacaacggc ggtacacgtg cttatgacgt
     1501 gccttcttat ggcgagctgc gtattggcga ccgggtgaag atcgagaaca accagctgta
     1561 ccgcatgcgt taagccactg cggcttcagc taaagccggc ctgctctctg gagcaggccg
     1621 gctttttatt gggctacttt aatgcagcgt gttgttgccg ggagcttgcg attcaagctt
     1681 gaaaaacccg gttcccatgg agccctggcc gatttccgcc tcggtggcct cgcgcacgga
     1741 ctcgaccttc aggctcaggc ggatcgcgat gccggccagc gggtggttgc catcgagcac
     1801 cacatgttcg ggataaattt cagtcacggt gtagatgtga tcggtcgggg tgtcggggtt
     1861 gacgccggtg ggcagggcgg cgccgtcaaa ggtcaggcct tcctcgagtt cggccggaaa
     1921 cagcttgcgg gcctcgagaa aaaccagatt ttcgtcgtag tcgccaaatg cgttttcagg
     1981 ctccagatgc agatcaatcg cggcacccgc ctcatggccc tgcaggcttt cctcgatttt
     2041 ggccagcaag tcatcgccgc ccagcaaaaa ctccaccggc tcgtcgagct cgtccagtat
     2101 ttcgcccagg gtatctttca gcgtccaggt cagggcaaca acgcattgcg gggtgatttt
     2161 catcggacaa ttgtcccatg gatgtcaatc aaccccttgc ccttctcgcc ggtctgagcc
     2221 cggcccaatt catgcgtcgc cactggcaga aaaagcccct gctggtgcgc caggccatgc
     2281 ccggtttcaa gcccttgctc agccgcaggg agctgttcga cctcgcggcg cgaaaagaag
     2341 tggagtcgcg ccttgtggtg cagcagccca ccggctggcg catgaagcag gggccgttcg
     2401 ggccgcgcag cctgccggcc ctgagccggc cgcagtggac gctgctgcta cagggcgtgg
     2461 acctgcacga tcgccgggcc catgaactgc tgcagcactt ccgttttgtg cccgatgccc
     2521 ggcttgatga cctgatgatt tcatttgcca cgccgggcgg cggcgtgggt ccgcactttg
     2581 acagttacga cgtcttcctg ctccaggcca gcggccgcag gcgctggaag ataggccggc
     2641 agcaagacct gacgctgcag gaaggtgtgc cgctgaagat cctgcagaac ttcgagcccg
     2701 agcaggagtt tgtgctggag gccggcgaca tgctttacct gccgccgcgt tatgcccatg
     2761 acgggctggc agaaccctgc ccgggggacg acggcaaggc gcaggactgc atgacgtact
     2821 ccattggctt tcgggtgccc aagcgcgccg aactggcagg tgaactgctg cagcgcctgg
     2881 cggattttgc ggatgatggt gaagacgatg gtgccgtcag tcagagtctg taccgcgatc
     2941 catcgcaggc cgccacgtca acacccgcgg ccctgcccga aggcctgctc gcctttgccc
     3001 aaaaggcagt gcatgaggcc ttgaaagacc cgctggcgct ggcctgtgcg ctcggtgaat
     3061 gcctgacgga acccaaggcc tcggtctggt ttgacgagcc gtcgggacga tgggacccgc
     3121 gctgcgcgga aggtctcagc ctggatccgc gcacccgcat gatgtacgac gcgcagcatg
     3181 tgttcatcaa tggagaaagc taccgcgcca gaggggctga cgcgaccctg atgcggcgcc
     3241 tggccgacca gcgccggctc gctgccgccg acctgcgcca cgcgagctct tcggcgctgg
     3301 acttgctggc agactggcat gcggccggtt ggctggccca tccacagggg cgctgaacca
     3361 cgcgtcaacc tgctgtttca tgccttcaag gagccgatga tgaatgatcc ccatccttcg
     3421 cccgcacctt cttcgcccat actgctgcag gggcgcttcg aagggcgccc ggcattcgcc
     3481 gatctgatcc ggcaggcctt cgcggcagcg gctgctgagg gctggcgtga aatcattctt
     3541 tgtgacgcca cgtttgagga ctggccgctg ggcgagcgca gtgtcgtgca atccctcaac
     3601 gactggtcca catcgggccg cacactgacc atgctggccc ggagctatga cgaagtgacg
     3661 cgccggcacg cgcggtttgt gacctggcgc cgcacctggt cccacattgt tgaatgtcgc
     3721 ggcagcggtg cggtggcggc cagcgacctg cccagtgcct tctggagccc gggctgggtg
     3781 ttcgaacggc tggacctcgt gcgcagcacc ggtgtggcgg gatccgaagc cgcgcggcgc
     3841 gtcgccctga gggaaaggct gacggagaag ctgctgcgca gcagtccggc cttcccggca
     3901 accacagtgg ggatttgacg cagcggggcg atgccgcagg gggctccgct ggcgacaggg
     3961 gcagttttgc catggtttct cgttgctctt tttctgaatt gagggttttt acggcggtga
     4021 aaacccgcat atactcgttc acagggcagt ttgagctcgc gtcacgcgcc ctcccgtcga
     4081 aaaccccgaa tcgtcaggtt tggggttttt tttgacgaat ttataagttt tctctctcat
     4141 tcatcaagga tctcgatatg aacaagtccc tcgttttggc cgccatcgtc gctgcggttg
     4201 ccctggctgc ctgcggtaaa aaagaagaag ccgctgcacc tgctcccgtc gttgtcacgc
     4261 cagcacctgc acctgctcct gctgaggcag cttctgctcc cgcagccgac gcatccgcac
     4321 ctgcgccttc gtccgctcct gtgcctgctg aagcccagac tcctgccagc gccacgaaat
     4381 aattcttttc gctcctgaaa ggccgcctcc gggcggcttt tttcgttggg aacagggcac
     4441 agcgcggtct gagaactgtc ttacagcctc agccagggag taccggtgcc ggcgtcggca
     4501 atcaccagct cggcgctgct gccgagggcc tcggtcatgg cctggtgcgc cagctcaggc
     4561 cggttgttct gctggctcaa atgggccgcc accagctgtc tgaggccgcc atgcgcgacc
     4621 gagcgggcaa tctcgcccgc agccgcattg gacaggtgcc cgtacatgcc gcctacacgc
     4681 cgcttgagaa aaggcgggta ggccgaccgg gccagcaagt cgctgtcatg gttgcattcg
     4741 agcagcaggg cattgcagtg tgcgaggtga gccagcaagt gggcggtggc gtggccgagg
     4801 tcggtcagca gccccagttt ggaagcgccg tccgtgcagg tgagttgcaa gggctcacgt
     4861 gcgtcatgag gaaccgtgaa gggcatgagc tgcagttcgc cgaggtcaat cgcctcccca
     4921 tcgcgggcgg tgtggagcag cccgtcgaag tcgggcaggc cgatgccctc ctgggtgcca
     4981 cggctcatcc agaccgggat ccggtggcgc cgcgcgaact ggcgcgcgca gccaatgtgg
     5041 tcgctgtgtt cgtgggtgat gaagatggcg tcgatgtcac cgtcggcgag gcctgcttga
     5101 gccagccgca ccgaaagttg ccggatcccc aggccacaat cgaccagcag ccgcttgggc
     5161 cgcaacccgg tcgcctccac cagcgtggca ttgcctgtgc tgccgctgcc caggcttttg
     5221 aacctcagca ccggacaggc tccacgcgac gccttatttc aggtcgtcgg caatgacctg
     5281 cacgatgcgc tgggcattgg ccgacgcttc ggggttgccc tgggcgttga gcacggacac
     5341 ggtggtcgct tcgccctggc tgcgtacggc gatccggtat ttgaggggag ccgggcctgc
     5401 cgaggagctg ctgaagagct tgctgaagaa gccgggttcc ttcttgtcgc cggtaggctc
     5461 gacgtagcgc acgaaataag tgccttgtgc gcggtcgcgg tcttccacgg tgaaaccggt
     5521 gcggtccagg gccagaccga cgcggcgcca ggcgcgctca aaaccctcgt cgatctgaac
     5581 cacgggcatg ttgttgacat tggccacccg tgcgctctgc cgcgccgggc ccgtggcgac
     5641 cagcgccttg gactgctcct ggctgacacc gagcttgacc atcaggcggc gcaggaattc
     5701 cgcttccagc tccggatcgg ccgggcgggg ctgccacacc gtgccatcac ttttctgcgg
     5761 tgtcttgacc gtacccgtca gcatctccac catgccgcgg tggctgatgt aaatttcggt
     5821 gccgccagtc ggcgtgcgct ccaggcgggt gcggaacttg tcgcgttcgc cggtggagta
     5881 caggctgtcg agcagcttgc ccagggtggc gcggatgaag tcctgtggca gcttggcgcg
     5941 gttttccgcg tagtcggttt ccatgatgcc gaggttttcc tgctcggtgg cgatcaggaa
     6001 gccgttttcc agccagaact cgcgcacggg gccccacagc ttgtcggccg ggcggttgac
     6061 caccagccag cgctgcgtgc ccgcgcgctc aatgcgcacg tcgccgaccg tgagggccgc
     6121 ggttggcacg gactgggtgg tctggccgac ctggtagccg ctggccgtga cggaactgcc
     6181 gggcacgacg taacgggtat cgcgcgacag ctgcgtcagg tcgggaggaa cctccagcga
     6241 gggcgctttg ccggcgcttt tgtagtcgac cttgtcgccc tgcatggact cgctcaatga
     6301 ggagcaaccc gagaggagga gcgcggcgac gagcgccagc agcggggccg cggcaagccg
     6361 tggtgcagag acaaatccgg aagcgctggt cgtgtgcaat gtcatcactg gggtttcctt
     6421 aaaaatcaga gcaggccgca aacgcgcagg gcgttttcga ccgtggcctc gttggccgtt
     6481 tcaagcggtg tcatgggcag gcgcagggtg ccgccgcaca ggcccatgcg ggccatggcc
     6541 catttgaccg gaatcgggtt ggcttccacg aacaggtgtt tgtgcagcgg cagcagctgg
     6601 agctggattt tcatcgcgct gcgtgcatcg cccgcgatgg cggccacgca gagctcgtgc
     6661 atcaggcgcg gcgccacatt ggccgtcacg ctgatgttgc cctggccgcc gcacagcatc
     6721 agtgccacgg cggtcgggtc gtcgccggaa tagacggcaa aaccggccgg cacttccttg
     6781 atcagccact gcgcgcgttc gatgttgccg gtcgcttcct tgatgccgac aatgccgggc
     6841 acctgcgcca gacgcagcac ggtggcgtgc agcatgtcgg ccacggtgcg gccgggcacg
     6901 ttgtacagca ccatgggcag gtccaccgct tctgcaatcg cggtgaagtg ccggtactgg
     6961 ccttcctggg tcggcttgtt gtagtaggga acgacctgca actggcagtc ggcgcctacg
     7021 gtttttgcaa aacgtgccag ctcgatggct tcggcggtgg agttggcgcc gcagccggcc
     7081 atgaccggca cccggcccct ggcttgctcc accgacacgc ggatgatctc gcagtgttct
     7141 tcgacgctga ccgtgggcga ctcgccggtg gtgcccacca ccccaatgca gtcggtgcct
     7201 tcggcgatgt gccagtccac cagtttgcgc agggtcggat agtcaacgct gccgtcttca
     7261 tgcagggggg tagccagtgc aacgatgctg ccggtaatgg ttttcatgtc tttgtatcaa
     7321 ggatggcacg ctggccgggg tcaatgctgc ggtaaagcca attcagcatt ctacccagtg
     7381 cgctggtcga tcaagtggcg tgggactgtc cccggcgaca gcagttcccg gaccgctgca
     7441 atccgctgtg taaagcggtc ggggccttgc tggaagccat cctcaaaggc gaccacccgc
     7501 aggccatggc agacctccag cagctcaccg gggcgcagca ggaaatcagg tcgcgagggt
     7561 ttccccacgg tttcgttgcc ctgggcaaag gtttcgtaaa tcagcacgcc gcccggcgcc
     7621 aggctggcca gcagcgtggg cagcaggggg cgccacaggt agttggtgac caccacggcg
     7681 tcgaactgcc ggcccgaaaa aggccagagt ccgttttcaa tgtcggccac cacggcttca
     7741 caggaggccg ccggaattgc tatggaatcg atagcttcct gcgcccgatc cacaagcgtc
     7801 agggggtgat ttcgctgatg aaaccagtaa
//
LOCUS       DAIDFG010000139         7784 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_139_length_7784_cov_18.111140, whole genome shotgun sequence.
ACCESSION   DAIDFG010000139 DAIDFG010000000
VERSION     DAIDFG010000139.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7784)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7784)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7784
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139_length_7784_cov_18.111140"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1460 a         2652 c         2286 g         1386 t
ORIGIN      
        1 cccgaaggcg gcggcggccc caattacagc ggcatgtagg gccatgcggg gcgggtccga
       61 accttcaccc gccgcgtcgc ccgggtgatg tcagcccccc accatttccg atcaacgtcc
      121 agagaagacc ccaactccca tgaacattgt cattaccggc ggcgccggtt tcctgggcgt
      181 tcgcctggcc cgcgaactgc tcaaacaagg cacgctctcg ctggccggca gccccgcgca
      241 ggccatcacc cgcgtgaccc tggtggaccg ggcggcacca ccggccgatc tgatggccga
      301 cagccgggtg cgcgcggtcg agggggacct gaacgtgctg ctcaacgcag acccggccgc
      361 cagccccgtg atcgccaggg acacagcggt ggtttttcac ctcgccgctg cggtcagcgg
      421 tgaatgcgaa gccgatttcg acctcgggat gcgcagcaac ctcgacgcca cgcgtgccct
      481 gctggagacc tgccgccgcc tgaagaccgc gccgacggtg gtgtttgcca gctcactggc
      541 cgtgttcggc aattcacccg aacagcccct gcccgcggtg atcgaagaca gcaccctgcc
      601 gacgccccag aacagctacg gcatccagaa attcatcggc gagcaactgg tggccgacta
      661 tgcccgcaag ggttttgtgc gcggccgcaa cgtgcgcctg atgaccgtga gcgtgcgccc
      721 cggcaaaccc aacggcgcgg cctccagttt cctgagcggc atgatccgcg aaccgctggc
      781 cggcgtgcgg gccgtctgcc cggtggcccc cgacacaccg gtggccctgt cctcgcccag
      841 ccgcaccatc cacggcctga tccgggccgc cgaggccagc gacgccgact ggggcgcgcg
      901 cactgccatc aacctgccgg ccctcaagac aacccccggc gagatggcgg cggcgctgga
      961 gcgccttgca ggcaaggagg ttgccgcgct gatcgactgg acaccggacg ccgcgattgc
     1021 cagcatcgtg accagctggc cggcccacat cgacgcggcg cgagcacgcc ggctgggctt
     1081 gctgcccgac ggcagtttcg acgccatcat cggagagtac ttaagcgaaa acccctaggc
     1141 ggcgcaacgc accagcaggt gaaaattgca agttgtatga caacttttcc ctccacccct
     1201 tttttcaccc cgaggagaca ttcaatgact tttttcaaac ccacgctgac ccgactcgcc
     1261 ttgggcatga cgctcgcact cggcctggtg gccggtgctg cggcgcagac gaccatgaag
     1321 atcagcatct cgacgtcaca gaactcccac cagggcgtgg ccatcgacac cttcgccaag
     1381 gaagtggaaa agcgcaccgg tggccgttac aaggtccaga ccttctacaa cggcgccctc
     1441 ggcggtgaac gtgaatccat cgaggccgtg cagctgggca cccaggaact ggctttctcc
     1501 tccaccggcc cggtccccaa cttcgtgccg gaaaccaaga ttctcgacgt gcctttcctg
     1561 ttccgtgaca aggcccacgc acgtgccgta ctggacggcc cgatcggcca ggagctgctc
     1621 gtcaagttcg aagccaaggg tttcaaggcg ctgggctggg cagaaaatgg tttccgccac
     1681 atgaccaaca gcaagcgcga cgtgaaggtg cccggggacc tgaagggcct gaaaatgcgc
     1741 accatggaaa atccggtgca catcgcagcc tacaagggct tcggcatcat caccaccccc
     1801 atggccttcc ccgaggtgtt caccgccctg cagcagggta cggttgacgg ccaggaaaac
     1861 ccgctgtcgg tcatcatttc cgccaaattc gaccaggtcc agaaacacct gtcactgacc
     1921 ggccacgtct attcgccctg catctgggtg atgaacaaag gcgtgttcga caaactcagc
     1981 gctgccgaca agaaggcctt cctcgaagcg gccgccgaag gcaccaaggc caaccgcgcc
     2041 cgcgtggacg aagacgatgc caaaggcgtg gccgacctgc gcgccaaggg catgaccgtg
     2101 atcgacaacg tcgacaaggc caagttcgtc gccgccctgg cccccgtgaa cgccgaattt
     2161 gaaaagcagt tcggcaaggc caacatcgaa aaaatccgca gcgtcaagta agctgccccg
     2221 ttcgctacac tttttgtagc cgaacacgcc cgcagccatt aggctggcgg gcgttttcct
     2281 tgcagaaccg ggattttcat gaaaaacacc tttctcagac tcgaacgctg gaccacgggg
     2341 tttgccacgc tggtggcctg cctcatgctg gtgatcgcgt cctcgctggg catgttccag
     2401 atcgtgacgc gcttcgtgct cgagcaacct gcagagtgga gcgagatcct gatccgcctg
     2461 tcgctgatct ggatggtctt catgggcata ccggcggcct tccgccaggg tgccatggtc
     2521 agcgtcgatg tgctctaccg ctggagcccc atcaaggtca aacgcatcct cgactgggtg
     2581 gtctgcgtgg ccgccctggc cctgatcggc gtcatcatct ggtggggctg ggactacgcc
     2641 gtccgcgggc aagtgcagtc catggccgga ctggagtcga tctccatgtt ctggggttat
     2701 gtggccatgc ccataggcgg cctgttctgc gtgatcggca tcatcggcaa cctgctggat
     2761 ccgcagcgcg aggaactgga gacggcgaca tgatccgcat cattgacaac aacactgaca
     2821 gcaacttttt cccggggatt gcccaatgac cgccgtcatg ctgaccacca tgatcgtgtg
     2881 tttcgcactg acgatctccg ttgccgtatc catcggactg gcttccatct tcggcatcca
     2941 ggcgaccaat gcccacatgc tgatctccgt caaggagatg ttcaacgcca tcaacaagtt
     3001 tccgctggcc gccattccgt ttttcatcct ggccggcaat ctgatggaga ccggcggcat
     3061 ctcgcgccgc ctggtcgagt ttgcgaaaag cattgtcggc ggcgtccagg gcggcctgcc
     3121 catgacctgc gtgctgacct gcatgatttt tgccgccgtc tccggctcgt cagtggccac
     3181 cacctttgcg gtgggtgcga tcctgattcc cgccctgatc aaacatggct accccaccag
     3241 ctacgccgcg gccctgcagg ccaccagcgc cgaactgggt gtgatcattc cgccgtcaat
     3301 tccgctgatc ctgtacggcg tcagtgccga ggtctcgatc ggcgagttgt tcatcgcagg
     3361 cgtcggcccg ggcatcctga tcagcctgtc gctgatggct tttgtctacg tgtattgcaa
     3421 gatcaagggt tggggcaaga acgacggcga cgggcgcctg ggtttcttca aggccacctg
     3481 gcaggccggc tgggcattgc tgatgccggt gatcattctt ggcggcatct atggcggcat
     3541 cttcacgccg accgaggcag cagccgttgc ggttttttac gcgctgattg tgggcggggt
     3601 gatctaccgc gaaatcaagt tgccgacact ggtgctggtg ctgcgcaagt cggtgatctc
     3661 cagcgccgtc atcatgttca tcattgccaa tgccgggctg ttcgccttcc tgatcacgcg
     3721 cgccggtgtg cccgatgcca ttggccgctg gctggaagcg gtgctacagt cgcctaccct
     3781 ctttctgctc ggcgtcaatg ccgcgctctt cttcatcggc atgttcatcg aaaccagtgc
     3841 cgccatcatc gtgctggcgc ccatcctggc tcccgtggcc atgcacttcg gcatcgaccc
     3901 ggtgcatttc ggcatggtga tggtcgtcaa cctggcgctg ggcatgatca cgccgccgtt
     3961 cggcgtgaac ctgtttgccg cctgtacggt agcgcgaata tcgctggacc gcattgtcaa
     4021 acacctgatt ccattcgtgc tggtcatcat cgcctgcctg atggtgatca cctattttcc
     4081 ggccatctcg ctgaccctgc gcgacctggt ctacgcccgc taggagcctg cccggcagaa
     4141 ggcatcgaag ctttgcagcc aaccttcctt ccgccgttca gacgcctggc ccgcccaggc
     4201 ttcatacgtg tgtcatattg tgtctgcatg atcggtgctt taccgttcag gacgccccat
     4261 gcagctccac aagacagacc gcgcccgggc cgagctcaaa tcgggaaccc gcacgctcgg
     4321 ccagcgcgag cgcaccctgc tgttgctggc cgatggcagc aaatccgtgc tggacttccg
     4381 gccgctcttc aatggggacg gcgaggaaat cgccctgcgc atgttgcgcg aaggttttct
     4441 gcagacccag cccggcaagg cgccgcaagc cgtccgccca ccggtgattg cggcagacaa
     4501 accacagccc gccaggtctg aaagacagcc acttccgcaa aacaatcctg ctccaccggc
     4561 acccgcccgg gtgtcagcag accagtttga cggaaaaagg tcactggcaa ccacacgaat
     4621 gttcctcttt gatatctgtg aacgcatgtt ttccaagcgg catcccgagc tggccgggac
     4681 cttccgcgat gccttgcgcg acgcccggga tcgcgacgcc atgctggcca cctcccgccg
     4741 catgatcgag gagatcgaga agatctcggg gcacgaacgg gctgactcca tcagtgagcg
     4801 cattgccatg ctgctgcctg ccgaacaggc ggcctgaggc cggcttcagc tacacttgcg
     4861 cggtcaggag agagttttcc tcgcgaaaac cgccgaaggc gcatgcggca ccccaatggt
     4921 gctctgaacg ctcaggcaaa aggactggca accgccaccc gggcgaacat cttccgggcg
     4981 gcgttcccta ctggagagag accaccttcg aggtggtcca ccgaaggagc aaacctgcat
     5041 cgccaggcga atctctcagg taaagaggac agcaggggca gcccatgatt tttcatggat
     5101 aacgacttgc cccggagaac gcctgtgtcc gccaccgatc ctgtcctgtt gaaaacaccg
     5161 ctgtacgacc tgcacctgga actgggcgcg cgcatggtgc cgtttgccgg ttacgccatg
     5221 ccggttcaat acccggccgg cctcatggcc gaacaccacc acacccgcca ggccgccggt
     5281 ctgttcgatg tctcgcacat gggccagctg cacctggtcg gtcttgaggc cgcagccgcg
     5341 ctggaaaccc tgatcccggt cgatgtgatc gacctgcccg ccggcaagca gcgctacggc
     5401 ctgctgctca atgaagacgg cggcatcatc gacgacctga tgttcttcaa ccgggactac
     5461 ctcaatggcg gcgacatctt cgtcatcgtc aacggtgcct gcaaggtggg cgacatcgcg
     5521 catatccggc aaaaaattgg cgcacgctgc gaagtcattc ccatgcccga gatggcgctg
     5581 ctggcactgc agggcccgca ggccgttacc gcgctgtcgc gcctggcacc cggcgtcgag
     5641 aaactggtgt tcatgaccgg cgggcgtttt accgtggcag gctgcgactg cttcctcaca
     5701 cgcagcggct acaccggcga agacggtttc gaaatttccg tgcctgccgc gcaggccgac
     5761 acgctggccc gcgccctgct ggcccagccc gaagtcaaac ccgtcggcct gggcgcgcgc
     5821 aattcgctgc ggctcgaagc cggcctgtgc ctctatggca acgacatcga caccaccacc
     5881 acaccggtcg aagccagcct gaactgggcg atccagaaag tgcgccgcac cggcggtgca
     5941 cgcgccggcg gttttccggg ggcagccaag gtgctggcgc aactggccga ccccgccacc
     6001 ttgacccgca agcgcgtggg cctggtcgca ctggagcgca ttcccgtgcg cgaccacacg
     6061 gccctgcaaa gcctggacgg cacgccaatt ggcgaagtca ccagcggcct gctgggcccc
     6121 agcatcgaca aacccgtggc catgggctac gtgaagcccg aattctcggc catgggtaca
     6181 cgcgtgaacg ccatcgtgcg tggcaaaccc gtgcccatgg aagtcagcgc catgcctttt
     6241 gttccgaatc gctatttccg cggttgaagt ttttattatt ctggagaacc ccatgatcaa
     6301 gtacaccgaa gaccacgaat ggatcaacgt tgacggcgac gtcgccaccg tcggcatcac
     6361 ccaccacgcg caggatgcgc tcggcgacgt ggtgtttgtg gacctgcctt ccgtcggcag
     6421 cacgctggca caaaaagaag tcgccggggt cgtcgaatcg gtcaaggccg cagccgacgt
     6481 ctacatgccc atcagcggcg aaatcaccga agtcaacgaa gccctgcgcg acgccccctc
     6541 gctggccaac accgacccgc tgggtgccgg ctggttcttc aaggtcaagt tggccgacaa
     6601 gtcgcagctg gacacgctga tggatgagac cagctacacc tctttttccg cgactgccta
     6661 aatagccctc ccgccccacc gttcggacga acgattcctg gaaactgcca tgttgatgcc
     6721 caccgccaag ccgctgcgcg agcttgaaaa cgcttccgaa ttcattgccc gccacatcgg
     6781 catcgatgag gccgacgaaa cccacatgct cagcgccgtg ggctccgctt ccaggcgcgc
     6841 gctgatcgac ggcatcgtgc cgcgcagcat tgcccgcagc caggccatgg ccatccccgc
     6901 acctgtcacg gaagcccagg cactggctga gctgaaagcc atggcggcga agaacaaggt
     6961 cttcaagagt ttcatcggcc agggttatta cggcacgcac acgcccggcg tcatcctgcg
     7021 caacattctt gagaaccccg cctggtacac cgcctatacg ccctaccagg ctgaaatttc
     7081 gcagggccgc atggaggcgc tcatcaactt ccagaccatg gtctgcgacc tgaccggcat
     7141 gcccatggcc aacgcgtcca tgctcgacga agccacggcc gccgccgaag ccatgaccct
     7201 ggccaaacgc agcgtcaaga gcaaaagcaa tgtcttcatc gttgcgggcg actgccaccc
     7261 gcagaccatc gaggtgatcc agacgcgtgc caggccactg ggcatcgagg tcaaggtcag
     7321 caccgccgcc gcaaccgtgc cgcagctgat ggccgagggc aactacttcg gcgtgctggc
     7381 gcagtacccg gccaccaccg gcagcatcca tgacctgcgg ccgctggccg gccaggcgca
     7441 tgtggacggc gccgccctgt gtgtggccgc cgacctgctg gcactcacct tgctggcacc
     7501 ccccggcgaa tgggatgccg acatcgtgct gggcaacacc cagcgcttcg gtgtggccat
     7561 gggcaacggc gggccgcacg cggcctacct cgcctgccgc gatgagttca agcgctccat
     7621 gccgggccgg ctggtcggcg tcagcatcga cgtgcacggc aacccgacct accgcctgac
     7681 gctgcagacc cgcgagcagc acatccgccg cgaaaaagcc acctccaaca tctgcaccgc
     7741 gcaggtgctg ccagccgtgc tggccagcat gtacgcggtc tacc
//
LOCUS       DAIDFG010000140         7710 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_140_length_7710_cov_17.068713, whole genome shotgun sequence.
ACCESSION   DAIDFG010000140 DAIDFG010000000
VERSION     DAIDFG010000140.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7710)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7710)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7710
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140_length_7710_cov_17.068713"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1354 a         2472 c         2531 g         1353 t
ORIGIN      
        1 ggaagacggt gtggtcgtgc acggcaccgt gtgggcgcgc gagatcggca tggtgaaaca
       61 ggcatgaagc tcaaacaact gttgacgatt tgtgcgatgg gcgccctgct gctgacccag
      121 gcgggatgtg ccggtgcggc agcacctctg gagctggggg gctacacgga cgacagcggc
      181 gccatcaccg tcctgcacgg tggtgattcg gcggacccct atttcgcgat gcaggccttg
      241 ttgctggcgc acgaaaacgg catggacatc tcggcgccgg cagaaaagtt tgcccactgg
      301 ctggtgccgc ggcaaaagcc ggacggcacc tttgaccggt tctgccgggg tgcggataaa
      361 aaatgggtgt cctgcaagac ggccgacgct gacgactcct tgctggcgat ctggatgaag
      421 ctgctggaaa ccatgccggc caagctggcc agcaacccgg tctggagcaa gagccaccag
      481 gccagcaagg ccgcgcttga gaacctgtac cagccgtcgc gcggcatcta catggtctcg
      541 ccggtggtgc tgcatggcct gttcatggac aacctggaag tctggtcgct caaggtgcac
      601 ctgaagcagc cgcagcaaag ggctgcgtcc gacaagctgg cacgcgccat tcatgacact
      661 ttctgggacc cggtcaacaa gcggtttctg gtgtccaccc agctggagca acgctcccag
      721 aagccggcgt tttaccccga ccatgtggcg cagatctttc cactgctgtt cgatttcccg
      781 ctggtgccgg gcaacgcgcg ccagcattac cgcgactgga tggcggccca ccgcgcggag
      841 tggctgatcc agggcgaagc cgactacccg tgggggctgc ttgcggtgct tgccatgcgc
      901 cagaacgaca aggccacggc cgcctgctgg ctgcgccagt ccacgccgct gcgccactcg
      961 tcgcgctggg cggtaagcga cgaagcgtcc taccagattc tggtgagccg gggtgtgacg
     1021 cctgcggcga aggatgcgaa ctgcaaatga acgaaggcgg gcgcggcgat gtgctgcccg
     1081 gccctgcgag caccctgtca gattattttt ttggagtgag ttttgatgtt taaacctgtt
     1141 tttcttgcgc tgccggtgat gctggccgct gcgatgccgg ccacggccca gaccgacgcc
     1201 ccggcgggcg cccccgcacc catggatgcc acggcggctc cggctttgcc ggggggcagc
     1261 gaagcgctgc gcagtctgga actcagcacg ggtgtgcaaa acctcagcaa tggcttcggc
     1321 aactggcgcg acgtgaccct gaagggcgtc tatggcgtgg acaaacatgt cttgcagggt
     1381 gagttgtctg cgcacaagcg gtttggggaa aacggtacgt tcctgagcct cggtgatacc
     1441 tataccttca atgaagactg gtttggatcg gtggcgctgg gcgtcggcga cggtgctttc
     1501 tatctgccgc gttaccgtgt ggacggaacg atttcccgaa aatttcttcc cggccgcaat
     1561 ctggtgggta cggtgggggc agggtattac aacgcgcccg atggccacac cgaccgcagc
     1621 ctgtcgctgg gggcagccta ctattttgag gcgccctgga ttgtggaggg tggcgtgcgg
     1681 ctgaaccgca gcaacccggg ttcggtggac acgcagcagc aatttgtggc gctgacctgg
     1741 ggacgccccc agcaggacct ggtgaccgca cgttacggat ggggcagcga agggtatctc
     1801 gccgttgccg ccaaccggca actggtcaac ttcgacagcc gcgaggccag ccttacctgg
     1861 cgccactggt tcaatgcgcg caccggcgtg ctggtcggcg ccaaccgcta caccaacccg
     1921 ctgtaccgcc gcagcggtgt gaatgttggc gttttccacg aattttgatc acgtcccatg
     1981 agctccagaa ccaaatccct gtctgaacct gcgcgtgagc aggagcgtct ttttaacgcc
     2041 tccttttctg ctgcccggga agcccgcgcc caacgggttc tggtgcgccc gcaccgctcc
     2101 atcacaccgc tgaatgtcac gccctggttg ctggcgcagg cgcttgtcct gccgctgatc
     2161 ctgtgcgcgc tggtgtattt cggaaaaccg ctgctgtttg atttctggcg cgattgcatc
     2221 ctgttctggt ccacggggct ggacatgcct ttcacgctga ccaaccagct caacgatgcg
     2281 gggcaatacg cgatgctgct gtcgagtggg ccggacgaca ggccgctgcc gagccgcacc
     2341 gtccttctgg tgacggcggt ggtaacgctg ctggggttgg ctttgtcggt acgcatgaaa
     2401 aacgcgcagc tgccgctcaa gtaccccctg cgcatcgtcg gcgtcatcca gcttgtcacg
     2461 ctggtctatt tctggttctc gccggacgct tttccctata gcattgcacg ccacagcgag
     2521 gaactcatga ccatcggcta cgtggtcatg ctggccacgc cggtgatgct ggcgatgggg
     2581 tattacatcc tgaaccagag cctgacgatc aagctgttcc acaccgcgtt gatcctgggc
     2641 ttctttgccg tcatgattcc ccaccaggtg ctggcgcagg ctttcatcat gcagcacctg
     2701 tcggtgctgt tcatgccggt gctgtacatc tgcatgggcg ccgtctttga tgcgctggtg
     2761 tttgttgccc tgtactcctg ggcggtcagc agcgcgccgc aagacgccac ggtctgacct
     2821 gtgtcctgac gtccgccgga gcggctcaat accggacgct gcagacctcc agcgcattga
     2881 gtccgcggtc agtgatgagg tagccgcctt cgccgtcatc ttccagaaag ccatgttttt
     2941 tcagggaggc gacggttttc aggccgtggg cgcgggtttt gtggggttgc ggctgcttgc
     3001 gctcgatggg cggcaagccg acgatcctga agccgtagcg ggcatcttcc agacactcgt
     3061 actgcttgta ggtcagtatt ttcaccggca gggcgccggt ggtggtgccg ctccactcga
     3121 actcgggcgg cgcgtagctg acccggtagg tgctgtcgtc cagtgtgctg ctctgcgtct
     3181 ccgcccgccc cccggttgcc ggcgggccgg agtcctggcg tttcatgacg cggtacatcc
     3241 cccagccgag cagccccagc acgatcaccg ccatgagcaa gacagcaaac tccacgaact
     3301 cccctttttt agcaagtggt gtaattatcc gtacaaagtg cccgcctggg tggacggtgc
     3361 tcagcgatcc ggaatatcca gctcgaagtg ggcatcaccc atgtagcgtt cggtgccaaa
     3421 agccgcttgc gtggccgcca gctcggccgc ttcccattgc tcggccagca actgttccgc
     3481 cggcgccagg tcccaggggc gggtctgctc tccatggatt tgcagcagcc ggaggtaggc
     3541 gcggtagacg ggtgccacca ggccggcgtc gcccagcgag gcctcgagtg cggtccgaaa
     3601 ccgctgcgcc gcgctggcgc gttcggtgtc ggagacatcc tcgctgtgaa aaatcctgac
     3661 cgtgtaatgc atggcaccag tctacaagct cacgcccggc aaccggtacg caggggcgta
     3721 gcgcacccgg tcatttcgtt actcatcttg cattagcagt ggagactttt taaaacgctc
     3781 tccttaccat cggttctttt tcaaggaaat ccagatggca ctgcaatcac cgttcttcgg
     3841 caaacgcgac aacgaacctt ccggtctgcg tcatggcagc cccaacccca atccctacgc
     3901 gaacggtcct gcggcaacca atgtctcgaa cagcgcgcct gctgccgctg cggccgcaac
     3961 cagcgcgccg tctgccgctg ccacccgtga gagtggcggc agcaagctga cggtcggccc
     4021 caacatcaag ctcaagggcg tcgagatcac cgactgcgac accctggtgg tggagggact
     4081 ggtggaggcc acgatggact cgcgcgtgat gcagatttcc gagcatggcg agttcaaggg
     4141 ctcggccgag atcgacatcg ccgaaatcca tggcgcgttt gacggcaacc tcacggtgcg
     4201 ccagaagctc gtgatttatg cgaccggcaa ggtcaccggc aagatccgct acggcaagct
     4261 ggtgattgaa gaaggcggcc agctcgcggg cgagatcctg ttcggagccg tggccaattc
     4321 gaaagccgca ctcaccggga agccgggcct gcaggtcgcc tgagtttttc aaacacccgc
     4381 atgctgtcac cgagttgacg acagtggcgc gccgggcggg cgcaaaatgc gccgatgtcc
     4441 gcggttcact ccccccttca ggctcaggtt gttcaatggc aggttgagcc cggcgctgcc
     4501 gtgcaggccg gcgacctgct ggtgattctc gaagccatga agatggagca cgaagtgcgg
     4561 gcgacttttg ccggccgtct cggcgaagtg ttttttgccg cgggcgagct ggtggagtcg
     4621 ggcgcgctgc tcgcgcgcat cgagcccctg ggggcgacgg cggcgcagcc tgtctctgca
     4681 cccgccgaag taacacccct cgcggtcagc ggcttgcgcg ccgacctgca gcgcacgctg
     4741 gatcgccatg tgcccacgca ggatgccagc cgccccgaag cggtggcccg gcgccacgcc
     4801 ctgggcctgc gcaccgcgcg cgagaacatc accgacctgt gcgacgagga ctccttcatg
     4861 gagtacggcg cgctggccgt cgcggcgcag cgcagccgcc gcagcgagga agacctggtc
     4921 aggaacaccc cggccgacgg catggtcacc ggcatcggca gcatcaacgg cgacctgttc
     4981 ggccccgagg cgagccgggc tgtggtgatg gcctacgacg ccaccgtgct ggccggcacg
     5041 cagggcatgc gcaaccacca gaaaaccgac cgcatgctgg ggatcgcgct gcagaacaag
     5101 ctgccggtgg tgctgtttgc cgaaggcggt ggcggccgtc ccggggacac cgacatgccc
     5161 atcgtcgccg gcctgcatgt tggcaccttt gcgagctttg cccgtctcaa tggccgggta
     5221 tcggtgatcg gcgtggcggc gggccgctgt tttgccggca atgcggcatt gctgggctgc
     5281 tgcgacgtga tcattgccac ccgggacagc aacatcggca tgggtggccc ggcgatggtg
     5341 gagggcggcg gcctcggcgt gttcaaaccg gaacagatcg ggccggccgg cgtgcagcac
     5401 ggcaacggcg tgatcgacgt gctggtggac gacgaagtcc aggcggtggc ggcggctcgg
     5461 cactacctgt ctttttttca gggtgcggtc caggcgttta ctgcgccgaa tgccctggcc
     5521 ctgcgcgagg tggtgcccga gaaccgtttg cgcgtgtatg acacccgcgc ggcgatcgac
     5581 ggcatcgccg acacgggcag tgtgctggcg ctgcgcgccg gcttcggcca gggcatacac
     5641 accgcgctgg cgcgtatcga ggggcggccc gtgggcattc tggccaacaa cccgctgcac
     5701 ctgggcggcg ccatcgacgc cgatgcggcc gacaaggctg cgcggttcat gcagctgtgc
     5761 aacgcgcatg gcctgccgct gatcagcctg gtggataccc cgggcttcat ggtcggccca
     5821 gagaccgaag cgcaggcgca ggtgcgccat gtgagccgga tgtttattgc cgcggcgcat
     5881 ctgcgcgtgc cgtttttcag tgtggtgctg cgcaagggtt atggcctggg cgccatggcc
     5941 atgacggccg gcagttttca ttcgccgatg ttcaacgtcg cgtggcccac cggggagttt
     6001 ggtgccatgg ggctggaagg tgcagtgaaa ctcggctacc gcaaggagct ggaggcacag
     6061 cccgaaggtc cgcagcgcga ggccttgtac gagaccctgg tggcgcagca atatgacaag
     6121 ggcagtgcgc tcaacatggc gaccacgctg gaaatcgatg cggtgatcga cccggcgcag
     6181 acgcgcgact ggctggtgtg cggcctgcag tcggcaccca ttcggcctgc ggatggcgga
     6241 ctcgccatcg atacctggtg aatccctaaa aaatgcggaa cagcagcggc agcagcagag
     6301 atgccagcac cacctgcagc cccagcgcca ggccggcgta agccccggca tcggcgttga
     6361 cttgcagcgc gcgcgccgcg cctatgccgt gcgacgcggt gccgagcgca aagccgcgcg
     6421 ccgtccagcc ggcggcatcc agcggtatcc cgaaggcatc aaacaggtat ttgccgctca
     6481 acgcaccgac caggccggtg atgaccgcaa acacggccgc cagcgccggg atgccgccaa
     6541 tcttttcggc aatacccatg gccaccggtg cggtcaccga cttgggcgcc agcgacagca
     6601 ccacgtccaa gggcagcccg acggcccagc ccagcgccag tgccgagccg ctggcggcca
     6661 tgccaccggc cagggcggcc agcagcaggc ggccccagcg cagccgcaac tcggcgcggc
     6721 gctgccacag tggccaggcc agcgcgacca cggccgggcc cagcaggaag tggatgaact
     6781 gcgcgcccgc gaaataggtt gggtaggaaa ccccggtggc cagcagcacc gccgcaatca
     6841 ccaccacggt ccagagtacc gggttggccc agggcgcctg ctgcaggcgt gcatagatgg
     6901 cctgggccag cacgtacacc accagcgtgg ccgtcaggcc gaacagcggc gtggcggaca
     6961 ggtagaccca gagatcaacg aacttcggca tttttccgcc acaggacatg cagggtcagc
     7021 accgtcacgg ccagcccgat cagcgtcgac aggacaatca ccagcaacat gcggccgccg
     7081 tactggctca ccaggctcag gtgggtcatg acaccgacac ccaccggcac gaacagcaac
     7141 gacaggtgcg ccagcagaaa gtcggcgcag gccgccaccg gctcgcgcac caccggccag
     7201 cgcagcgcga ccagcagcag catcatgccg atgacggggc cgggaaaggg caaggacagg
     7261 ccgcgcgaaa gcagttcgcc gatggactgc agcagcagca accaggccag gccgcgcaag
     7321 ccctgcacgg ctaaaacctc gggagatcag ggtgcgacat cttgcctccg cgcaccagca
     7381 ttttcccgta ctcggcgcag cgctgcagcg tcgggatcac cttgccgggg ttgagcaggc
     7441 cctgcgggtc aaacgcgcgc ttgacaccga acatctgctc gttctcgctg gccgagaact
     7501 gcacacacat cgagttgagt ttttccacgc ccacaccgtg ttcgccggtc accgtgccgc
     7561 ccatggccac gctggtctcc aggatgtcgg cgccgaacag ctcgcagcgg tgcaactggt
     7621 cggcgtcgtt ggcgtcgaac agaatcagcg ggtgcaggtt gccgtcgccg gcgtggaaca
     7681 cattggcgca gcgcaggccg tacttgattt
//
LOCUS       DAIDFG010000141         7645 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_141_length_7645_cov_20.764954, whole genome shotgun sequence.
ACCESSION   DAIDFG010000141 DAIDFG010000000
VERSION     DAIDFG010000141.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7645)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7645)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7645
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141_length_7645_cov_20.764954"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1372 a         2514 c         2388 g         1371 t
ORIGIN      
        1 cgacccagaa caaactgctg aaaattgtgc tgtttggcca gcccgagctg gacgaattgc
       61 tggccgcacc gcaactcagg caggtcaagg acaggatcag ccaccggttt gaactgcaac
      121 cgctcgatgc ccaggacgcg tcggcctacc tgatgtttcg cctgcacaag gccgggtgga
      181 aggggggcga gctgtttgac gccgcagctt tgcgcctgct ggttcaggcc tccgagggcc
      241 gtacccgcaa gctcaacatg ctggccgaca agtccatgct ggcggcgtat gcggaaggta
      301 tgccgcaagt gggtattgac caggtgcgcc gcgcggtcgg cgacagcctg ccgaccacca
      361 ggcgcgccac gctgtttgcc gcaaacatag ccagcggcag ctccgctggc ctgtggcgca
      421 ggtttgcgcc ggcaggcttc ggggtgctga tcgccttggg tgtcgggtat cttttgggca
      481 gtggacgcgg gccggacagg agtgagcgca cggcgatgac ccagccggtg acgccgctgg
      541 tgcctgaggc ggcttcgcgt ccgtttgata gcctggcgcc acactcgccg tcgccgccgg
      601 ttttgcccgt caaagcggct cccggccctg tcctggccgt tgccgccgct gcagccccat
      661 cccgcagtcc agccattggg gccgtgcctg ctgccacgga ttccctgccg cctggggcca
      721 gaaacgcgtc gatcgcggag cccgcgctgg ttgcgaagct gaaggacagc tccctggccg
      781 ttcgcatgca ggccactgac gagttcatcg ccaagccctc cagcaagggg ttcacggttc
      841 agctattgtc ccagcgcgcc ccgcgggatg ccgaagtact ggccatggcc aatgaagtcc
      901 aggcagcgct ggacggcgct gcgcagcccg ccgtgccggt gctcgtccac aaccggctct
      961 accagcgcca actctctcac gccctctatg cagggcactt cgaaacccgc gcggacgccc
     1021 tggagttcat acaatcagct cctgctgcca tcaggcgcta taacccggtt gtgcgcagcc
     1081 tcgacgccat ccgtaaggag cctgtccctt gaaaatcgat tgccccgcct ggaagctggt
     1141 cttgctggcc gcactggtgc ttgcaggctg tgccacaccg ccaccaccca tgtccaaagg
     1201 acacctggtc gaggagcccg gcacggtgcc accactcgcc cagccgcctg gcgacgtcgt
     1261 cagcattccg ccgctttcgg ccggcgcacc gcgtctgccc gcaccccggg cggtcaaacc
     1321 gcaggaaacc tacagcgtgg tggtcagcaa cgtcaacgtt cagaccctgc tgtttgcatt
     1381 ggcccgcgat gccaagctga acctcgatgt tcacccgggc atcaccggca ccgtcacgct
     1441 caatgcgctg gaccagacga tttccgaact gctggatcgc attgcgcggc aggtcgacat
     1501 gcgttacgag ctggacggca agaacctgtt cgtgctgccc gacacgccgt ttctgcgcct
     1561 gtacaaggcc gactatccca acatctcccg cgattcggtc agcagcacgg gctccgagtc
     1621 gcaggttgcg accaccgggc agggcactgc gtcgggcacg ggcaactcct cgtccgcgtc
     1681 aattcgcaat acgtccaaca accggttctg ggtcacgctc acggaaaaca tccgtgattt
     1741 gctccgtgaa accgacaagg tcatcgaaac gacggctgct gctgcacctg ctgtagtgcc
     1801 cgctgcgcca acgcctgtgg cctctgtggc cggggcgctg ggtgcgaccg cccgaaccat
     1861 cacaggcgct gctacgccag cggctgctgc cgcaccacaa ccgctttacc gcgaggccgc
     1921 atcggtgatt gccaatccgg agacgggtgt gatcagtgtt cgcgccacag gcaagcaaca
     1981 tgcacgcgtg cgcgagtttc tcgaccaggt ggttggcaat gccaggcggc aggtgctgat
     2041 cgaggccacg attgtggaag tggcgttgag caacgactac cagcagggaa ttaactggtc
     2101 gttcgtccgg aacgcggtct ccacaccgct gagcctggtg atggccgccg gcggtggaac
     2161 agccctgttg acgggcgtca acccagcttc tcttggtacc tttaccgccc agaaatcctt
     2221 cggaaaaacc gatatttctg cgaccgtgcg cctgctcgaa tcattcggga agacccgcgt
     2281 gctttccagt cccaaggtca gtgtcatgaa caaccaggcc gcgctgctca aggtggtgga
     2341 caacaaggtg tatttcacga tctcggtgac accgggcacg ccaggctcac ttggtgtggc
     2401 ccccactgca gcggtttaca ccaccaccat caacaccgtg ccggtgggct ttctgatgaa
     2461 cgtgacggcc caggtcggcg acgacgacga aatcacgctg aacctgcgtc cgtccgtgtc
     2521 ccggatcatg agttatgtca atgatccgag tcccgccctg gcgacagccg gtgtcaccaa
     2581 ccggattcct gaaatacaga cgcgcgagtt cgagtccatc atgaaggtac gggacggcga
     2641 gaccgcggtg ctgggcggtc tcatgcagga cgaccagagc aacgccacgg accagcttcc
     2701 cggcgtgggc aatgtgccgg tcgtcggcga actgttcaag ctccgcagca accagtccaa
     2761 caaaagcgaa ctggtcattt ttctgcgtcc caccatcctg cgcgactcga gcattgatgg
     2821 cgacgcctcc tttatccgga gtcagctttc catcggcaca ccggtgcctg tggggcgccg
     2881 gccatgagct tgttgctcga cgcgctccgc cgggcagagg aggactcccg caagcgcaag
     2941 ctggacgcgt ctgcgaccgg cgccggcccg gcgcccgtgg cggacacacc tgaggaccag
     3001 gcgcagacat ccggcgccga gttgaccttg caggcgcaga caccgccttc gataccaacc
     3061 caggaaccga ccggagctga tatgcacgct tccgcggcgc cactggtacc ggcgcccgag
     3121 acgggttttc cggacctgac gctggtgtcg gacgaacccg ccgccgccgc gccgttgccg
     3181 caagccgcac ccgagaccgc gaccgcggtg gccgatcccc ggaccccgac tcagccctac
     3241 ggacgttatg ccaggccaac ggttgccgaa gcaggccaga gtacgggggc tgttaccggg
     3301 ggctgggccg ccgcccaagc ggcgcgcgaa ccagtgcgcc cctcaacggg gcccttcgcc
     3361 gggcgagttc ccgtgtccca agcccccgaa acaacgccga ccgcgacggg gtccctggac
     3421 cgggttgcac ccgttccggg aaccacgcag cgccaggcga tctcggcagc gagcgcgatg
     3481 gcgggtgccg gcgttcgcaa ggtgccgaac aagccgcagc gacggcaatg gttgcttgcc
     3541 gcggtggcgc tggtggtggc actgcctctg gccgcctttt tgctgtttgg ggatgtgctt
     3601 ttcgggtcct ccagcacact gcttgcgagc aagcccatcg tgccagtcgt ggccccgcag
     3661 gtcgctgcgc ccgatgcatc tccggcatcc ccatctgatg ctgctgcgcc cggcaatctg
     3721 cccgctgcgg ccagtgaaac gacggcggtt gccgggacgg acaagagcgc ccctgccgcc
     3781 gcacagccgg ttgccggcat ggtcccgccg tccgcgccaa gggtggctgg cggcccaacc
     3841 cgcacatctt ctggcccggc gcctgtgatg tcgcgtcccc gcgcagtggc gcaaaatcct
     3901 gcgcgcaaca ccgcggccag gcagacgcca atcggcggcg acagccgcct tgagtcgggt
     3961 gccgcccctg gcggacctgg tgtggcattg cgtcccaatg ccgcaagacc tgcctcgctc
     4021 atggatgccg cgtacgcggc ctaccaggcg ggcaagacca ctgaagccac ccggctctat
     4081 caggacgttc tcagggccga cccgactcag cgtgatgcct ggctcggcct ggccgtcatt
     4141 gcacatgccg ccaatcagcg cgaaccggcc atggatgcct acaagcgggt cttgcgcctg
     4201 gagccgcaaa acgccacggc gctcgccggc ctcagcagcc tgatcaccaa cgcgggcgaa
     4261 ccccagcagg agtccaggct ccgggaaatg ctggcccggt cgccgcagga ggccgatctg
     4321 aacaatgccc tggggctggt cctgtccggc gaaaagcgct ggtccgaggc gcaacccctg
     4381 tttttcaagg cgcattccgc agcgccccag gagccgcaat tcgcctacaa cctggcggtg
     4441 acgctggacc acctgagaaa gtccggcctc gcggcgcagt actacgaaac cgcgataggg
     4501 ctggcccagg gacgggcctc ggggtttgac gaaaccagcg cacgcaccag gctggctgca
     4561 ttgaaggcgg gcgcggttgc gggggcgtcc cgatgaacgc caaactcgac gtctacaacc
     4621 cgacgtcgtt ttaccttgag ctcgagcgca gcggcctgat cacgcacgac caggccgaga
     4681 ttgcctggac cgagcgtgaa aagcgcggcg gctctttcgg cgacatcctg gtcagcctgg
     4741 gcatgttgtc gctcgatgac ttgacggcca tcatggctgc ggccagtgcc accgaggcga
     4801 tccgcctcga cgacatggtc cccgacgacg gggcgatcaa actcatcccg aaaacgctgg
     4861 cgcagaacta cgacgttttt ccggttggtt ttgacgacga gagccgtcag ttgacggtcg
     4921 ccatcagcga caccaacgac gtcgtggcca tggaccagtg ccgctccgcc gtgccggccg
     4981 atatttcact gcgctggcga ctggcgtcca aagccgacat caaggccgcc atcgaccgct
     5041 tctacggtta tgtgctgtcc atcgacgaca tcctcaaggg gctgcgcacc ggcaagatgg
     5101 acgacagcgc actgtccatg cggggcgcgg aggcttttgc gcacccgatt gtccggctca
     5161 tcgatgcctt gctggccgat gccgtgctcg aaggtgcttc cgacattcac ttcgaacccg
     5221 aagaaggttt tttgcgaatt cgctaccgca tcgacggcgt catgcgtcag gtgcgtgtgc
     5281 tgcacaagca gtactggccg gcattgtcgg tcaggctcaa ggtcctgtcg ggcatggaca
     5341 tcgccgagac gcgggcaccc caggacgggc gtatttcgat ggtcatacgt gggcacgatg
     5401 ttgactttcg cgcggctgcg cagccgacca tccatggcga gaacttcgtc ctgcgtgtgc
     5461 tcgaccggcg caagtccatc gtgcccatgg acaagatggg gctgcctgcc gaccgcatga
     5521 acaccttgcg gttgatgctg gcgcggccct acggcatcat ccttgtgacg ggaccgaccg
     5581 gcagcggcaa gaccaccacg ctgtactcca tcctcaacga gatgaacacc gagagcgtca
     5641 acatcatgac gctggaggac ccggtcgaat acccgatggc gcgtatccgc caggcctccg
     5701 ccgccgacaa ccccaagatg gattttgcca acggcatccg ctccatgatg cggcaggatc
     5761 cggacatcat tctggtcggc gagatccgcg accacgagac ggccgagatg gcttttcgtg
     5821 ccgccatgac gggccaccag gtgttttcaa ccctgcacgc caattcggcg gtgctggcca
     5881 ttgcccggtt gctggatctg ggcattcccg ttagcgtgat gacgggcaac ctgattggca
     5941 tcgtcgcgca gcgcctggtg cgtctgctgt gccccaagtg caaggctgcc tacgaagccg
     6001 gcgatgttga aaagtcgctg ctgggcgttg cacaccatct gccgctgacc ctgtaccgcg
     6061 ccgtggggtg cgcccactgt aaaaaccagg gttacaaggg gcgaaccagc atcatggagc
     6121 tgctgcgctt cgacatgggc ctggacgacc tgctgtcgcg caacgcgcgt acgcccgagc
     6181 tgctggccca cgcgcgcagc aacggtttca ccaccatggc cgaggacggg ctgggccgcg
     6241 tgctggcagg cacaacctcg cttgaagagg tggcccgtgt ggtggacctg acccggttgg
     6301 ccaactgatg ctggagttca attaccgcgc gatcacggcc gagggccgga ccatctcggg
     6361 gcgccagtcc gctaccagcg agtcggacct ggaagcgcgc ctgggccgca tgcagctgga
     6421 gctgctgaaa gccacgccgg tacgcgctgc cacgctgttt gcccgcaaga agctggagag
     6481 caaggagctc atcaacatct tcatccactt gcagacgatg agccatgcag gtgtgccctt
     6541 gtttgaatcg ctgatcgatt tgcgcgactc tgccgacaca cccaccgtgc ggcgtttcat
     6601 ctcggacctg gtggatcgta tcgagggcgg cgccagcctg tcggaggcgc tcgagcagtc
     6661 gcctgcgggc atcgacaagg tcaatgtcaa cctgattcgt accggcgaag ccaccggcaa
     6721 gttgccggag gtgctggccg agcttgtgga aagcctgaag tggtcggacg agattgctgc
     6781 gcagacaaaa aagctgttga tgtatcccgt ctttgccggc accgtggtgc tcggagccgt
     6841 tggctttctg atgatctacc tcgtgccaca gttgcttggg ttcatcagga atctgggcgg
     6901 tacagtgccc atccagacac gggcactggt ggctttttcc ggctttctgg tcaattactg
     6961 gtacaccatt cccctggtga tttttctggt tgccgccttg tggatcacgg ccgtgcgtct
     7021 gtctccggct gccaggctct gggtagacag tgccaaattg cgggcgccca tctttggccc
     7081 catcctcaag aagatcatca tggcgcgttt tgccaagtcc tttgggctga tgtacaaaag
     7141 cggcgtctcg gtactcgacg cgctcgggtt ctgcctgagc ctctcaggca acgcggcgta
     7201 tcgcaaggcg atcagcggcg ccgcgctgag catttcccaa ggacagaaag tgtcggactc
     7261 cttcgccgcg accacgctgt ttccagcatt ggtggtacgc atgatccggg tcggcgaaag
     7321 cactggcggc ctggacaagg cgcttgaaaa cgtcagttat ttctacaccc gcgaagtcaa
     7381 cgatgccatt gaaaaactgc aggggctgat acagcctgtg ctgacgctgg tgctcggcgg
     7441 catgatcgcc tggatcatgt cgtccgtgat gctgcctata tacgacctga tttccaaggt
     7501 caagttctag ggcctgccca cactatttat gcgagatgcg ttgcgagtca aaaaggtcat
     7561 gcgcgaggcg caaaatgcag ccgggctgga gcccggcaag gctttgcaac gctgcgcatg
     7621 gcctttttgg ccgcaacccg gaggg
//
LOCUS       DAIDFG010000142         7586 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_142_length_7586_cov_18.772407, whole genome shotgun sequence.
ACCESSION   DAIDFG010000142 DAIDFG010000000
VERSION     DAIDFG010000142.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7586)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7586)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7586
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142_length_7586_cov_18.772407"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1242 a         2703 c         2431 g         1210 t
ORIGIN      
        1 ctccgacagc aacaaggacg gcggcatttt caccagcaac tgggagctgt acagcgtcga
       61 gatcgcgctg gtcgaattgt tcgaccagct cgccaactcg cacaacatcc agctgcgcat
      121 gttccacggc cgcggcggca ccgtcgggcg cggcggcggc cccagctacc aggccatcct
      181 ggcacagccc ccgggcacgg tgcgcgggca gatccgcctg accgagcagg gcgaagtcat
      241 cggctccaaa tacgccaacc cggaaatcgg ccggcgcaac ctggaaaccc tggtggctgc
      301 cacgctggaa gcgaccctgc tgcagcccac caagccggcc accaaggcct ttctgcaagc
      361 ggccgaccag ctgtcgcagg ccagcatggg cgcctaccgt gcgctggtct atgagacccc
      421 gggcttcacc gagtatttct tcagcgcgac accgatccgc gagattgccg aactcaacat
      481 cggctcccgg cccgcttcca gaaaagccac ccagaagatc gaggaccttc gcgccatccc
      541 ctggggcttc agctggggcc agtgccgcct gaccctgccg ggctggtacg gctttggcgc
      601 ggcgattgcg cagtttctcg accagggagg cacaccggcc agtcgcaagg aagcgctcgc
      661 cctgctgcag aagatgtaca agcagtggcc gtttttccgc accctgctca gtaacatgga
      721 catggtgctg gccaaaagcg acctggcgct ggcctcgcgg tatgccgaac tggtcggcga
      781 cgcgcgcctg cgcaagaaga ttttcagcgc gatcgaggtc gagtggcacc gcacggccga
      841 agcgctgacg ctgatcaccg gcgacaagca gcgcctggcc aacaacgcgg ccctgcagcg
      901 ctccatccgc caccgctttc cctacatcga cccgctgcat cacctgcagg tcgagctggt
      961 gcgccgctac cgcgaaggca aggccgacga acgcgtgcag cgcggcattc acatctcgat
     1021 caacggcatc gcggcgggct tgcgcaacac cggttgagct gcgctgcgca ggcggaggtt
     1081 gctattgatt cagtagctgc tggcgcactt ttcataaggg ctggcggcct tttttcctta
     1141 gaatttccgt caacggtgat gggcgcaaca aagcgcagaa aatgacgctt tagccaacaa
     1201 ggtccctgtg aatccatcca ctgcccatgc cgaactcatc gccacctaca ggagggcgca
     1261 ggcagaggct gcccacaagg ccggattgat caaagcggtg gcaagcaaag ggcccaaggc
     1321 gattcaggcg gccgtcgaaa cagcctccaa ggcagcaaaa cgcagggatt cgtatgcgaa
     1381 aaagctggca cttctggggg tggacctcaa ggactgagcg tcagaccccg aagcgcgggt
     1441 cgggttgcgg ataatcggga tttggaaaga accccgatgg gaattgaaga accacaagaa
     1501 ccggaagaaa tggaagaacc ggagccgctg gaggaatacg tccctggcat cgcgggcggc
     1561 cggcactaca tggcccggct gtgtcatgtc cctgacggtc cctggtacat agccgtcatt
     1621 cacgtggaat caatgccgcc tttgcacgac agcgacagga cctggcccac ccgtgaggag
     1681 gccgttcagg cggcaaacaa gctggtggca gacctgggtc actgagccgg gatcaccggt
     1741 tcgccgccat gcctgcaagc cggcccgttc gctccggcgt ccagccatgc gattttcctg
     1801 tccagaaacg acagcatgga ctgccgttct ttttctggca ggcccggtcc gcgcggaaga
     1861 tccggcggca ataagcccgc ggaccacact gtcggactac tcctacaggt cttccagtgg
     1921 aatccgatgg cggcgaccgc aggtgaaacc taagatgggt tttaacgcca cacggcattg
     1981 cccaaggcat tgctggcgct cacccccctg gagcctgaac catgtctacg ccacacgccg
     2041 aggcggtcag ccgcctttcc gcccaccatg attccatgag caatgcgcat gtgagcagtg
     2101 acttcacggc actggccgca cacatgaacg attgccagct ttcgcgcggc cgcttttttg
     2161 cactgcgtgc cgcactggag tccctgcacg ccaccgcgtc cccgcgcatc gtgaccacag
     2221 gcgccctctt cgtctgctgc ggcgtagcct tgctggccgt ggtttgagag ggtgaacgcc
     2281 gctgttcttg accggctgga atcactcacc ccgcacgcac gccagatcct cctgacccag
     2341 aggtcggaag ccgatccgcc cattcgcgcc gaactgttcg gcatccaccg tttcgaacaa
     2401 catggccgca gcctggccgc ggcccagacc gtgcaggccg acggcccctc gcggcgtggc
     2461 ccttcctttt ttccgcgcgt cgaggaaaac ctggtcgcgc tgcgccaggc ctacgactat
     2521 gtggcgctga cctcgagcag cggccactat gtgacgcctg ctgccgaatg gctgctcgac
     2581 aactttcacc tcgtcgaagc ccagctgcag cagatccgcg agggcgtgcc gcggcgttac
     2641 tacgcgagcc tgcccaaact ggcggcggca ccgctggaag gcctgccgcg cgtctacggc
     2701 atcgcctggg cttatgtcgc gcataccgac agcgtgctca acccggaact gttcaccgcc
     2761 tttctcaatg cctaccagga atacagcgaa ctgcgcctga gcgagctctg ggccctgccg
     2821 accaccctgc gcgtggtgct gcttgaaaac cttcgccgca tggccgacaa cattgcccaa
     2881 agcaaggtgg cccgggaagt cgcgcatgcg gtgtgggact gcgtggacag cctcgacgac
     2941 gccgacctcg acgcgatcaa cgccctgatg cagcagcggg gcctgcagcg caactacctc
     3001 acgcagctgt ggcagcgcat gcccacggtc aaactggaca tctcgccctc gctggttctc
     3061 tggaccgaac ggcattgtcc ggacgggccc gcgctgatgg ttgaaagcca gaccgcgcaa
     3121 gtggccgcca acctcaccgt cggcaacatc atcaccaccc tgcgcctgat cggacaggcc
     3181 gactgggtcg agctgatcga gccggtaagc cggtcgctgc gcatgctcag ccggttgccc
     3241 agctttgccc gggaaagcga gctgacgcgc cagcagatca cccaatccat ggagcggctg
     3301 gcgcgcctga gccgccagtc cgagcgcgag gtggcgcagg ccgtggtcgc cgccgcgcgg
     3361 gcggtgcccc acaccctgcc cgcgaccgcc gtggagcgca cggccggcta ctacctgctc
     3421 ggcggcggcc gggccgtcct ggaggcgacc ctgggcatga cggcacgcgg cgcgccggcg
     3481 gccgccctgc gcaactggcg cctgccgctg tacatcgctc ccatcatcct gggcaccgtc
     3541 ctgatcgtgc tggcggcggc caaccgcatg aacctgcaca gctggccgac ggtggcggcg
     3601 ctgttcctgc tggcctggcc ggcgtccgaa gccgccgcgg ctttcgtgca ccgcctgatc
     3661 gccgaatcgc tcaagatccg cgccctgcca cggctcgatt tcgcggccgg catcccgccg
     3721 gaacaccgtg tgctggtggt cctgccgacg ctgctgagct ccagcgcaac cattggcgag
     3781 ctggcagaac ggctggagct gcactggctg gccaaccgcg agcgcgaggc ccagtttgcc
     3841 ctgctcaccg actgggtgga tgcaccgcgg gaaaccatgc cgggcgatgc ggccctgctc
     3901 aacgcggcgc tggcgcaagt cgccgaactc aatacgcgcc accccgcggc ggccgggctg
     3961 gcaccgcgct ttctgctgat ccatcgcccg cgcgaatgga gcgacaccca gcaaaaatgg
     4021 ctgggctggg agcgcaagcg gggcaaactc gaacagctgg tgcgcgcact ggtcaccggc
     4081 agcatggccg gcttcatgcc gctggccaac gaccagcggc tggccgacga catccggtac
     4141 atcgtgacgc tggacagcga caccggcctg ccgcccggcg ccctgcgcga cctggtggcg
     4201 gtggcggctc acccgctgaa cacaccgcag gtcgacgccg cgtcgcgccg cgtggtggcc
     4261 ggttacggca tcctgcagcc gcgcgtggtc acaccgctgc cggggcccgc tgaacgcacc
     4321 ccctttcacc gcctgtttgc cgggcaatgc gggattgacc cctacagcaa cggcgtgtcc
     4381 gaggtttacc aggacctgtt cggcagcggc agtttcagcg gcaagggcct gctgcacgtc
     4441 gccgccgtac atgcggtcct cgacgaccgc ctgcccacgg gttccatcct cagtcacgac
     4501 ctgctcgaag gcaacatcgc gcgctgcggc tttgtcagcg acgtgacgct ggtcgaggag
     4561 cagccccacc atgcgggcgt ggccgcctcg cgcattcacc gctggacccg cggcgactgg
     4621 cagttgctgc ccttcatggc gcgcgccagg cactacggca tcgacgcact gggcctgtgg
     4681 aagatggtcg acaacctgcg ccgctcgctg gtgatccctg ccaccgtcgg tctgctcgtc
     4741 tgcgttgttt tcacggcggc catgcccttg tccacggccc tgctcgtcac cttcgcggcc
     4801 ctgctggccg ggcccctgct cggcgcgctg gccggcctgg tgccgacacg ccgtggcatt
     4861 gcctggcgcc attttttcca tgtgggccgc agcgagatgc tgcgtgccct gggcggcgcc
     4921 gcctggcagt tcagccagtt gccggtgcag gccaccctgc tgttggacgc catcgtgcgc
     4981 tctgtctggc gcatgcgtgt gagccgccgc gggctgctcg aatggagcac cgctgcccag
     5041 gcccaggccg ccgcacgcca cgagctgtct gccttcgtac gccagcatgc gccggtgtcg
     5101 ctgctgtgcg cggcgctggc ggtcgccgcg ttgtggagcc catacccggc tgctggcacg
     5161 gcgctgttcc tgctgtgggc ggcgtcgccg ctgggcgcct ggtgggccag caaaccgcgt
     5221 ccgcccgatc ccgcgcgccg cctgcgggat gaggaccggg cctacctgca cacgctggcg
     5281 cgcgacacct ggcgcttttt cgagcaatcg gtcggcgccg aggacaacca cctgccgccg
     5341 gacaacctgc aactggtgcc cgagcccacg ctggcgcatc gcacctcgcc gaccaacatc
     5401 ggcctgtacc tgctggccat ctgctgcgcc cgcgagttcg gctggatcag cacgaacgaa
     5461 gtcatcagcc ggctgcaagc ctcactggac agcatagacc gcctgcccag gcacgatggc
     5521 cacctgctca actggtacga cacgcgaacg ctggaggtgc tgcagccggc ctatgtgtcg
     5581 gtcgtcgaca gtggcaacct ggccggcctg ttgctggccg tcgcgcaggc ctgcaccggc
     5641 ctggcgcaaa gcggcgccga cggcaagccg gcgcaggcca gcctgctgca gctggccgcg
     5701 cgctgccagg ccctgtacgc agccatggac tttcgcggcc tgtacgacgc caagcgccac
     5761 ctgtttcata tcggcctgcg cgtgaacgaa catgtgctcg acgcgagtta ttacgacctg
     5821 ctggcctccg agtcccggct gaccagcttt ctggcgattg ccaagggcga cgttccgcgg
     5881 cgccactggg ccgccctcgg ccggcccttc ctgtcggtgg gcgtggtgcc gggcctgaag
     5941 tcctggtcgg gctcgatgtt cgaatacctg atgccctcgc tgctgatgtc cgagcccggt
     6001 cacggcctgc tgcacacggc ggcccacgcg gctgtggccg agcagcgcgc ctacggtacc
     6061 gcgcagcaac tcccgtgggg cgtgtcggag tccggctatt tcgcgcagga ccattcgctg
     6121 gccttccagt actccccttt cggtgtcccg cggctggcgc tgcggcgcac cccgcacacc
     6181 gaccgcgtga tcgcgcccta cgccaccctg ctggccacac tgttcgatgc gcgcggcgcg
     6241 gtgcgcaacc tgcagcgcct ggaaaaactg ggcgcacgcg gcgagctggg gttttacgaa
     6301 gccctggact tcaccgtcgc ccgccagccg gaaaagtcgg gcaccgaggc gttcagcgtt
     6361 gtggaagcct tcatggcgca ccaccagggc atggcgctgg tggcgctgtg caacgtgttg
     6421 tgcaccaacg caccgcggcg ctggttctgc ggcgacccgc tggtgcgggc acatgaaagc
     6481 ctgctgcatg aaaacacgcc gcgccagatc gtccgcagtg ccgacccgcg cctgccgccg
     6541 gagcccggcg gctacacgcc ggagccggtg ttccagtccc gcgagctgaa cccgctgggc
     6601 agcggctgga aacccacgca cctgctgtcc aacggcaact acaacgttgc tctgcgcgcc
     6661 aacggcgccg ggctgagccg ctggcgcggc cgcaacatca cgcgctggcg cgatgacctg
     6721 ctgcgtgacg gctacggcag ctttttctac ctggtccatc ccgaccaggc cggtgttccc
     6781 agcgtcagct cgctcacggc gcggccggcg ccgggcccgg gctggcacta ccaggcccgc
     6841 ttcatggccg accgggtgca gttcgacgcg cagggcgcag acctgagcac caccctgacc
     6901 gtgctggtca gccccgagga cgacaccgag ctgcgcaccg tgacgctgct caataccggt
     6961 cgcaccgagc aggtgctgga actggtgtcc tgctttgagg cggtgctggc cgacccgcgc
     7021 gccgacgaag cccacccggc attttccagc ctgtttgtgc agacccgctg ggagccgcag
     7081 tggcgcgccc tcatgctcac gcgccggccg cgcctgcacg gcgacccgca aatggcggtg
     7141 gcgcattttc tggccgaggc tgacgccgac ctgatctcgg tggcgtgtat cgccgaccgc
     7201 cgcgtattcg ccggacgcca ccacccggca caccagccgg ccctgggcga gcagcccttt
     7261 gatgcgcagg gccatcccgt caacggcctg gacccggtgg ccagcctgcg cgtgacgctg
     7321 cggctggcgc ccggcgccct ggcccgcttg agttttgcca cgacggccgc cgagacggcc
     7381 gacgagctgg tgccccgcat cgacaaatac ctgcaaccca tgcacgtgga gcgcgcgacc
     7441 cgcatggccg cgacgctggc ccaggtgcgg ctgcgcgacc tggcgctgac tccggaggaa
     7501 aacctggcgc tgcaggacat gagcacggcg ctgatgtaca gcgtgggccg gccggcggaa
     7561 cgcacgccgc tggaccagcg ccagct
//
LOCUS       DAIDFG010000143         7580 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_143_length_7580_cov_16.000532, whole genome shotgun sequence.
ACCESSION   DAIDFG010000143 DAIDFG010000000
VERSION     DAIDFG010000143.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7580)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7580)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7580
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143_length_7580_cov_16.000532"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1354 a         2485 c         2400 g         1341 t
ORIGIN      
        1 ggtgtttgtc accgaaggca gccagcgccg cgaaccggtg gcctccatgc ctggcgtgga
       61 acggctcagc ctggatttgc tgctgccggt ggccgaagag tgtgtgcagc tcggcattcc
      121 cgtgatggca ctgtttccgg tgatcgacaa ctcgctcaag acttacgacg gcaaggaagc
      181 cttgaacccg gacggcctgg taccgcgtgt ggtgcgcgaa ctgaaaaaac gttttcccga
      241 gctcggcgtg atgaccgacg tggcgctgga ccccttcacc agccacggcc aggacggcct
      301 gccagacaag gaaaccggct acatcctgaa cgacgagacg attgagctgc tggtcggtca
      361 ggcgctggcc caggccgaag ccggcgtgga catcgttgcg cccagcgaca tgatggacgg
      421 ccgcattggc gcgatccgcc aggcgctcga atcgcgcaag ctgatccaca cccggatcat
      481 ggcctacagc gccaaatacg cgagtgcgtt ttacggcccc ttccgggatg cggtcggctc
      541 ctcgggcaac ctcggcaagg ccgacaaaaa ggtctaccag atggacccgg gcaacagcga
      601 cgaagcgctg cgcgaagtgg ccatggacat tgccgaaggc gccgacatgg tgatggtcaa
      661 gcccggcatg ccttacctgg acgtggtgcg ccgcgtgaag gacgagttcg gcgtaccgac
      721 ttttgcctac caggtcagcg gcgaatacgc gatgctcaag gcggccgcac aaaacggctg
      781 gcttgaccac gacgcggtga tgatggaaag cctgctggcc ttcaagcgcg ccggcgccga
      841 cggcgtgctc acctacttcg ccatcgcggc agcgcgtttg ctacaaaaaa catagccatc
      901 tacgcatgac tgacaagggc tagaggccaa tttgaccatc aaccggacag acccccgtgc
      961 aaatagttga attcaccgcc gacagcctgc gcttttccga caccctgccg gcgcaggcgt
     1021 cggcggatgg ttttgtctgg ctgttccttg accgcgaaga attcgagacc cacgaggcgc
     1081 tgctgcatca ggcggctcag cagctgggcg gctcggccct gctggacctg cattaccagg
     1141 acttgcgcaa cccggtccac ccatcgcatt acgactacac ctccatctac gacctgatca
     1201 tctttcggcg gctggccacg caagtcgaaa cccgggccga ggtcgaacac gagacggcgg
     1261 tcgatgccta ccacggccag agcagccagc cgcgtaccaa gcagcgcggc ggcctgcctg
     1321 ccttcaaccg catcagctca cgggccgtgg gttttgtggt gtttgaccgg ctgctgatca
     1381 gcgtgcaccc gcgcggctgc tacaccgcca agagttttat ccagcgctcg ctgaccgacg
     1441 ccaagttcag cgtggacagc gtgtcggcgc gcagccgcat cccgtccagc ccggctgatc
     1501 tggtgttgcg catggtcaat gccatggtgg acagctacct cgacgtgcgc aaggaactca
     1561 ccacccagct cgagcactgg caggccgagc tgctcaaacc caattcgcgc ttcatcaact
     1621 ggggctcgct gatgggcgca cgcagccagc tgcatgtgct ggaagacctg tgtgacgagc
     1681 agcgcgacgc gatgcaggag tggctcgact ccttgcgcga gcagccgatg tcgtcctttg
     1741 ccgccgatga acagctggcg ctgacacggc gcgaccacat ggtggcgcgc gcgcgcgacg
     1801 tgattgaaca catcgagcgg gtggtgcacc acgcgcgccg gctggagcaa tccgccgaaa
     1861 cggtggtgca gatccacttc agcgcccaga gcaaccgcac caacgacatc atgcgcacgc
     1921 tgaccgccct gaccgccgtg tttctgccgc tgaacctgat caccggcttt ttcggcatga
     1981 acttcgagtt catgcccctg attcaccaga ccagcggctt ctggtggacc ctcggtttca
     2041 tggtgctgct ggtggtggtg gtggtgtcgg tcttctggcg caagcgctac ctggccagaa
     2101 gcaggcgtta ggaaccaggg gcatggcggc agtggtgcgt atcggttgcg ccggctggag
     2161 ccttccgagg gacagctggc cgcggttccc cgacaccggc acccacctgc agcgttacgc
     2221 ccagcggttc aacgcggtgg aaatcaattc gtcgttctac cggccgcacc agccggccac
     2281 gtatgaacgc tgggctgcgg cggtgcctgc cggcttccgt ttttcggtca agctgcccaa
     2341 aaccatcacc catgaaaagc gcctgcgcga ctgcgcatcg ctgctgcacg gctttctggc
     2401 ccaggcctgc gcacttggcg accggctggg ttgcctgctg gtgcaactgc cgcccagcct
     2461 ggcactggac acccacgtgg cgtccggctt tctgcaaacc ctgagaaagc agcacaccgg
     2521 ccccattgca ctcgagcccc ggcatgccag ctggtttgcg ccgtccgcgg acgcgctgct
     2581 gcgttcgttt gaagtgggtc gcgtgctggc cgacccggtg ctgtttgaag cgggagcgcg
     2641 gcccggtggc tggcccggcc tggtctacct gcgcctgcat ggctccccac gcacgtacta
     2701 ctcgccctac ggccacgccc tgctggacca gctggctgcg cggctcgtgc tggaagcggc
     2761 gacggctgac acggtgtggt gcatttttga caatacggcg tcgggcgcag ccacagaaaa
     2821 tgccctttac acctgccgcg cggtacaggg tccgggcacc tgacagcggc atttcctcca
     2881 ccgcaagcca aaggagttgc gatgagttca gccgccacag caagacccac cccctatggt
     2941 gatcccgggt ccgccgcaga accgacctgc gtcgacgacc tgacgcgtca gaacgtccag
     3001 gccatgcagc gcctggaaga agccgcactc gcctcgcgca gccgttccga ccgcatcgcc
     3061 ggcttcattg cccgcttttg cggctccatg ccttttgtcg gggtgcacgt ggcgatattt
     3121 tctgcctggc tactggtcaa ttcactgcct gcgttgcccc acttcgaccc ttacccgttt
     3181 acctttctca cactggtggt gtcgctcgaa gcgattttcc tgtcgacctt catcctgatt
     3241 agccagaacc atgagctgcg gatcagcgaa cggcgcaacc agctggacct gcagatcaac
     3301 cttctgacgg aacaggaaaa caccaagatg ctgcagatgc tggagcgcat cgccagaaaa
     3361 gtcggcgtgt cgatggacga cgatcccgcc gtgcacatcc tgaaaaaggc gacacatccg
     3421 gaggccctgc tggaacagat cgagcaggca cagcgcgacc gctccgaggc gcccaactag
     3481 ccgggcgccg ccgctcagac ccagggctgg gcgatctgct cggtttcgaa gacccgcaac
     3541 accgcaggcc gcgccagcat gcgctgcagg taaggcccga ggtgcggacg cgtgcgggcg
     3601 gcgggtgact ggaagttgcg cgtccagcgg cacagcgtga acacataagc gtccagcgcg
     3661 ctgtacttct caccggcaaa ccaggggcca ccatggcgtg ccagttcagc gtccagctgg
     3721 tcgagcatgc cgccgatttt tgcttcggcc tgcgccttga cctctgccgc gccggccgca
     3781 ttgccggacg ccaccgagcg ttctggatag aagtaaacaa tcagcgtggc ctgcaaggtg
     3841 ttggtcagcc acatcagcca tttgtagaag tgcgcgcgcg ctgccgtgcc caggggcggt
     3901 accagccccg cttcgggatg catgtcgctc aggtgcaggc agatggccgc ggtttcatac
     3961 aacaccaggt cgccatcgac cagcactggg atcaggccgt tcgggttgag cttgaggtag
     4021 tccggcgcct tgtgggcgtt ttgcatgcgg tccaccagca cgcgctggta aggcacgccg
     4081 atttcttcaa gcacgatgtg cggcaccatg gctgcggtgc tcgggtaata gtgcagactg
     4141 atcatggcac tttgacgcca aacagggtcg ccccgttgtc ccagccgatt tttcgtgcca
     4201 cgtcgggcgg caagtcaccg agccagctgc ggtaaccctt catgagttcg tcgtagtact
     4261 gccagcgctg gttgacccaa gtgtccgagc cgatcatgaa acgccccggg tacttgagca
     4321 gcaaggcgcg ccactcgggg cacagcttgc ccgagtcaca ggtcaaaccc ggccggtagg
     4381 acagctcgcc catcaacgtc gggtagcggg ccagcagcgc ctccacctgc gccacgggca
     4441 cgccgctgat gccggtgtgc gcccagatca gccgggcctt gggcgcatgc gccatcagca
     4501 ggtcaatcgc cacatcgtcc acatgcgcga ggatggcgag attgttcttc ccggcgaact
     4561 gcatcagctt tttcgccacc ggtccgttgg cattggcact gtcgtacaga tggaattcgc
     4621 cgatgccctt gtacggtccg gcctgggcgc cgctgacgcc gcgcgcaaac tcggcctgca
     4681 ccatctcgta aatcgtctcg tcgcgatgcc agagttcgta gtcggcgcgg ttgcggtaca
     4741 ggcggatgaa gggcaccacg gtgacaccgg cgcgcgccgt ctgctccgag gccgccagcg
     4801 ccttggtgcc gtcgttgggc cgcgagttgg cgacgatggc cttgacgcca ttgcgctgca
     4861 tacgcgccag cacatcggcg ggaccatggg gccccacact gccgttccag gcctcctcgt
     4921 tgtaatgcag gtgcgcatcg aacagcgggc cgctgtagtc ggtcgcctgg gcgaacgccg
     4981 aaaatgctat caaaaacaga gctgtcggcg cccgccagtc aatggccaga ggccaaaaac
     5041 acctgaagcg cgggcgaccc atggttcagc ccaggtggtt ggcgaaaaac gccagcgtgc
     5101 gctgcttcgc cagcgtggcc gcggcctcgt tgtacgagcc gcgctggtcg caattgaagc
     5161 cgtggttggc ttggtagaca tgtaccgcga cgccgggctg ggctttcttg aaggcctcga
     5221 cggtgtccag cggaatccag tggtcctggt cgccgaagtg aaccatcacc gggcaggccg
     5281 gctggcgcgc gggttcatca cccgccgtca tgccgccgcc gtaatacggc acggcggccg
     5341 acaggccgga cagcgcggcg gccgcgcgcc aggtcagcag gccgccccag cagtaaccga
     5401 cgatgccgac ctttttgccg cctgtgcttt tcgccgcgtg gtcgatggcc gcctgcacat
     5461 cctgcagcac gccggacgtc ggcagggctt ccaccgcggc cttgagttcc atcccggttt
     5521 tcatgtcggc gtcggtgtag ccgatgtcca cgccaggccg gacacggtgg aaggtggcgg
     5581 gcgcgaccgc catataaccg gccgccgcgt agccgtcggc gaccgagcgg atgtgcgaat
     5641 tgacgccgaa aatttcctgc agcaccacca cagcggcttt cggcgtgccg gccggctcgg
     5701 ccacgtaggc cggaaaaaca aaaccgtcgg cggaagtgag atcgatgaat ttgcccatgg
     5761 cgcaagtctc ctgttcagtt gctgatgaat gcttcaattt ttcaatgtca acttcaattt
     5821 acttggcagc gagttttttc tcaagcagat ccagccggtc ctggccccag aaaatctcgc
     5881 cgtcgaccac gtagctgggc gcaccgaaca cccccgcatc gatggcctgc tgggtgcggg
     5941 tttcatacag ctgctggatg gccggactgt gcgactgggc caggcgccct gcatccaggc
     6001 cgcgctcctg cagaatttcc gccagcaccg cgtcgtcggc aatattgcgc tcttccaccc
     6061 agaccgcctt gaaaatcgcg ccggccagcg ccatggccgc agcactgccg tcggcctggt
     6121 ccaccgccgt gatcagccgc gcggcggggt cgccccccac gggaaagtat ttgggctggc
     6181 ggttgatcgg caactgcagg tgctcgccga agcgctgcag ttccacgagt cggtaggcct
     6241 ggcgctgcgg cgcgcgctgg cccaacggca ggccgccgga gaccggaaag attttgccga
     6301 aatccaccgg cagcacccgc accgccgcgc cggcggcgct ggcgatctgc gcgaaacgct
     6361 ggtggcccag gtaagtccac gggctttgcg gcgtgaagta atagtcgatg gtggtgctca
     6421 agggtgtctc cggtggaaag ctgttttgcg gccgttgcgg ccgattgagg tagattcggg
     6481 tcaacccggt ttgtaaccgg gattgcattt ttttgctgga ggccggcttt gtccaacatc
     6541 gttctgatca ccgggggctc acgcggcatt ggcgccgcca ccgccctgca ggccgcccgc
     6601 gccggctatg ccgtggcggt caactacacc gccaactcac tcgccgccga cgaggtggtg
     6661 cggagaatac gccagggcgg aggcacggcc atcacggtgc aggccgacgt agccgaggaa
     6721 agccaggtgc tggcgatgtt tgaaaaagtc gatgccaagc tcggccggct cacggcactg
     6781 gtcaacaacg ccggtgtggt ggaccagacc tcgcgggtgg acggcatgag cgtggcgcgc
     6841 ctcaagcgca tgttcgacat caacgtgatc ggcaccatgg tctgcgcgcg tgaagcggtc
     6901 aaacgcatga gcacgcgcta cggcggcgcg ggcggcgcca tcgtcaatgt ctccagcgcg
     6961 gccgcgcggc tgggctcacc cggccagtat gtggactacg cggccgccaa gggcgcggtg
     7021 gacgtcttca ccatcggcct ggccaaggaa gtggcggccg agggcatacg cgtcaacgcg
     7081 gtgcggcccg gcatcatcga gacagacatc cacgcctcgg gcggcctgcc cgaccgcgcg
     7141 cgcgacctgg cgccgcaggt gcccatgcag cgggcgggca gcgccgacga agtggcgcaa
     7201 agtattgtct ggctgctcgg cgatgcatcg agctacacca ccggcgcgtt gctggacatc
     7261 gcgggaggcc gttgatggac ttcaaaccgc ttgtcaccct gctggccatc gtcaacccgc
     7321 tggccatcgt gccgtttttc attcattaca cgcggggatt ttcgacagag cagcgacggc
     7381 gcaccatcgt catcgcctcg ttcagcgcct tcatggtgat tgccaccagc gcgctgctgg
     7441 gcttgcagat cctcgagttc ttcagcatct cgctggccag cttccaggtc ggtggcggca
     7501 tgctgcttct gatcagcgcc atgaacatgc tcaacgcgca gccggccgaa gccaagccga
     7561 acacccacga actcgaagac
//
LOCUS       DAIDFG010000144         7574 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_144_length_7574_cov_15.994547, whole genome shotgun sequence.
ACCESSION   DAIDFG010000144 DAIDFG010000000
VERSION     DAIDFG010000144.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7574)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7574)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7574
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144_length_7574_cov_15.994547"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1295 a         2409 c         2456 g         1414 t
ORIGIN      
        1 cgccaccgcg caggggcaga tcagcaaccc gctgaccctg gctttttcaa gcgtgctgtg
       61 tgccatcggc tcggtaattc tgtatgtctg ggtcaacccg ctgacgatgt ggctgacctt
      121 tgcaaccttt gtcggttatg cggtggtcta caccgtgatc ctcaagccgc tgacgccgca
      181 gaacatcgtg atcggcgggg cctcgggtgc gatgccaccg gtgctcggct gggccgcgat
      241 gaccggcgcc gtgagccccg aagccctgat tcttttcctg atcatcttcc tctggacgcc
      301 gccgcacttc tgggcgctgg cgctgtaccg tgtggaggac taccgcaagt ccggcctgcc
      361 gatgctgccg gtcacccacg gcaacgagtt cacccgcctg caggtgtttc tgtacacgct
      421 ggtgctgttc gcggcctgcc tgatgccttt tgtcttcaag atgagcggct ggctttacct
      481 ggtggtggcg attggcctga gtgtcggctt cagctggtac ggctggcgcc tgtggcgcaa
      541 ttactccgat gccctggcgc gcaagacctt ccgcttttca ctgatccacc tgtctgtctt
      601 atttgcagcc ctgctgctgg accattacct cgtatgagca cttcccccac ttcttccggc
      661 aaacgcaaca ctattaaatt gatagctgga ggcgcttttc tggcgggcgc tgcaggcctt
      721 ttaggcgcat gctccgggga aaagaagccg cagttcacca gcatcgacat caccggcgcc
      781 gactacgccc gcgactttgc cctgaccgac cacaacggcc aggcgcgcag catcaaggac
      841 ttccagggca aggtggtggt catctttttt ggtttcaccc agtgcccgga cgtgtgccct
      901 acctccatgg ccgagctcgc cgagatcaag aaaatactgg gtgccgacgg cgcgcggctg
      961 cagggcctgt tcgtctcgct ggatcccgag cgcgacacgc aggaagtgct caaggcctac
     1021 atgggcaact ttgacccgac ctttctggcg ctgcgccccg atctcgacaa gctgccgcag
     1081 gtggccaagg acttcaagat cttctacaag aaggtcgacg gcaagacgcc cggtagctac
     1141 acgatggacc actcggccgg cagctacatt ttcgatccgc agggccgggt gcgcctgtac
     1201 agccgctacg gcaccggcgc ggcgccactg gctgcggaca ttcgcctgct gctccagggc
     1261 aactgaagcg ccgcccggca cgctgtctgg agcggggcaa aaaaaagcct gcagacgatg
     1321 gtcgaatgca ggctttggca atcaccgctt gcgcggtgtt gcagtcaagg tatcaggcac
     1381 ggtcaggcat aagcggccaa gacctttttc atttttttca tcgccgccac ttcaatctgg
     1441 cgaatgcgtt cggcgctgac cttgtattcg gccgccaggt catgcagcgt catgccgcct
     1501 gagccatcgt cgttgacctt gagccagcgt tcctcgacga tgcggcgcga acggtcgtcc
     1561 aggccttcca gcgcagtggc gatgccgtcg ctggccagat ggtcgcgctg gcgcgactcc
     1621 atcagctgcg tcggttcctg cgtggcatcc gagaggtagg caatcgggcc gtaggcttct
     1681 tcgccgtcgt cgctcgggct cgggtccagc aacacgtcgc cgccggacat gcgctgctcc
     1741 atctcgatga cttcctcgcg cttgacgttc agtgttttgg ccatcgaatc aatctggcct
     1801 tcggtcagcg tgtcgcggtg cgtcgccaca tcggcgtcgt ccttgaaacc ctgtttcatc
     1861 gagcgcaggt tgaaaaacag cttgcgctgg gctttggtcg tggcgacctt gaccatgcgc
     1921 cagtttttca ggatgtactc gtgcatctcg gccttgatcc agtgcagcgc gtagctcacc
     1981 aggcgcacgc cctggtccgg gtcgaaccgt ttgaccgcct tcatcaggcc gatgttgcct
     2041 tcctggatca ggtcggcgtg gggcaggcca taacccaagt atttgcgcga agtggccacc
     2101 accagccgca ggtgggacag caccagcttt ccggccgcgt ccagatcgtt ctcgtccttc
     2161 agtttccggg caaattcctg ctcctgctcc agtgtcagca tgggcaggcg gttgaccgcc
     2221 gaaatgtagg catccaggtt gcccagcggt ggcagcagcg accatggatt ggcaggcgcc
     2281 aactgggttg tcgcgggggc cacttgaggg gacagagcgt aggacatagg gtgaaactcc
     2341 tttatagaag taatattagc actctgttgg atagagtgct aagcgatagg ttcaatggtt
     2401 ttgatagggc aaggctaata agtaataacc cttgtttgtg tcccattttc cgctttagtt
     2461 caaccgcttt tgtaggccag gatgcagtga aagaggcgtc caaaggcccc ggtctggcgg
     2521 tggcgcaggc gccctccggg tggaacaggg cggggggagc aggggccggc agtgccggat
     2581 ttcatgcatg aaatcggtct ccagcccaat acagtcaagc ggggatagct attgtttgca
     2641 tagcaaaatg gagccggtgc atggccccca aggaggcgga aaccggttca ggccccgggg
     2701 gtcatcaggg caggcgcttg tcgctgcgaa tctcgcgcac ttcaacgtcg accacgtcgg
     2761 cctcgcgcgc cgcggtggtg ccgggtttga cggcgcccgg ccaggcggct tgggacgaga
     2821 agcgctggaa actgccaaac acgacgggtt tgggcttctg gccggtgatc agtgagcgca
     2881 gcaggcccag cgccagcagg acaaggccgg ccagcatcag gcttaccgcg aagatggcgg
     2941 caaaggtcca caggaccaga cgcaggagaa aggacaggac gcggctgagg aagctgggca
     3001 cgtgagggtt tcccgtaaga gtcagagcag cttatatgcg ggcaactacc gtcgcttcaa
     3061 gcccggtaga gaggcgtaga agctaccagc ggacccccag cttgccgaac agcttgctca
     3121 gcacgaacag cgggcccacc cagaggtact ggatgtcctc gaaaaacgaa ggctttttgc
     3181 cttcgagctt gtggccgaca aactgcaaaa tccaggcgcc gacaaaaatc gccagcgaca
     3241 gcggcagcac gcgctcaccc atggcgtgca ccagcgccag caggatggcc gaccagagca
     3301 ccatggttgc cagaaaaacc agcgacagcc gcaggtagta aaccatgctg gcggcgacaa
     3361 aaagataggc aacccaggga tgcagcgcaa acatcatgcc gagcaggctc agcatgatga
     3421 gcgggatggc aatgaagtgg atcagctcgt tcttcgggtt ctggtggctt tcgccgtaat
     3481 gggcgagcag gcggtccacc tcgcgtggat cggcggatgc gtcggatgat gtgctggggt
     3541 tcatgtggcc tccggtgccg cggtcgaaaa gccgccggct agcgggtcac tgtaagggat
     3601 cgctgcggtt tgcgaaagca ggggaattac tgcattttcg ccagccgatg cagttgttcg
     3661 tgcagcgcat cgggaatcgt cagccccgat tgtccggctt gctgttccag cgcgattctg
     3721 cgcgcgccgg ccaggcgcac accgtcgtcc accagcattt ccgcgatcag cgtttccatg
     3781 cgttcgtaat agacgtcctg gcccgccagc gcgcccgggt tgatcacgac aaaggcctgg
     3841 ccaaggcgcg gcgcgttgcc ctcgtcgaca aaaaacgagg aagcttcaaa gccgaactgc
     3901 gcaccgagca gggccgtgac cagcagctcg acgatcagcg ccagcatggc gcctttgggg
     3961 ctgctggcag cgcccagcgg cagcatggag cccgccaggc cggctttcgg gtcggtggtg
     4021 ggtcgcccct ggctgtccag cgcccagccc agcggaatgg cctggccctc gcgggctgcc
     4081 accatcagct tgccgcgcgc gacttcgctg agggacaggt cgatcatcag cgggtcggcg
     4141 ctgcgccgcg gaaaagtcgc tgcgaccggg ttggtgccga agatggcgtg tttgccgccg
     4201 gccgccggca tggcggccgg cgagttggca aaagccagcc cgaccaggcc ggcgtcggcc
     4261 actgcccgca gatgatcgac cagcacaccg cagtggtggc tgttggtcac gccggcgaag
     4321 ctcacaccca gcgtgcgggc ggtcgcgatc gctgtggaga cggccagatc gcaggccgga
     4381 aaggccaggc cctggccggc atcgaccagc acggccgccc cctggtgtcg catcacccgg
     4441 ggcacggcgc tgccgtcggc ccggccgtta cgcaggtggg tcgcgtactg cgccacacgg
     4501 atcaggccat gcgaggccag gccttgcgcc tcggcgcgca ccagcgcttt tgcggtgctg
     4561 gctgccatgg cctggcccgc gccggcatgg tgcagtgcct gcaaggccaa gcgggtggct
     4621 tcttcaaggg acaggtgggc catggtttca ggttctcagg ccagcagggc ctgcgaaaat
     4681 tcccgggcgt tgaaggtttc gaggtcttcg agtttttcgc cgacgccgat gaaataaacc
     4741 ggcacgggcc gcaggcccgg cgaggccttg gcgcgctcgc gcgaccacag cgcaatggcg
     4801 gccagcacgc cgcctttggc cgtgccgtcg agtttggtga cgatcaggcc ggtcagctgc
     4861 agggtctcgt cgaaggcctt gacctggctc agcgcgttct gtccggtgtt gccgtcgatc
     4921 accagcagca cttcatgcgg cgccgtggca tccgccttgc tgacgacacg tttgatcttg
     4981 cgcagctctt ccatcaggtg caactgcgtc ggcaggcgcc cggcggtgtc caccagcacc
     5041 acgtccttgc cgcgtgcctt gccggcgctg accgcatcga aactcacagc ggctggatcg
     5101 cccccctcct ggctgacgat ctccaccatg ttccggtccg cccaggcttc gagctgttcg
     5161 cgcgcggcgg cccggaaggt gtcggccgcg gccagcagca ccgacgcgcc ttcgttggcc
     5221 aggtggtgcg tgagcttgcc gatcgaggtc gtcttgcccg caccgttgac accggccacc
     5281 atgatgaccg tgggtttgta ttcgccgatc accagcggct gctccagcgg cttgagcagt
     5341 tgggtgagcg cctcggtcag gatgttcttc acggccaccg gatgggtgac gccgctgtct
     5401 ttcacgcgtc gcttgacttc atcgagcagg taggcggtgg cgccggtgcc ggcatcggcc
     5461 atcagcaggg ccgactccag gtcttcgtac agcgcgtcgt cgatgcggtt gccggtgaac
     5521 acgccggaaa tgctggcgcc ggtcttgtgc aggccagagc gcagcttgct gacccagtgc
     5581 tcgcggctcg ccggtatcgt tccgggcagc gggatgtcga tgggcgtgac cagcgcactg
     5641 ccgatcagtc caccgccagg cagcgccggc gtggctgcag ccggggccgt agcgggtgcc
     5701 gcaccgccgg gcagggcagg gggcggtacc gctgccggcg cggcctcagg gggggcaggg
     5761 ggcttttttt tcttgaagaa actgaacatt ggttgtgctt tggagtcaca ctattctatg
     5821 aaacacacca cacaggattt catgggcagt tttatccggc ttccggcacc ctcgcgcaac
     5881 acgcttctcc cccttctttc cctgctcttc agcttctttc tggccagccc cgcggtggcc
     5941 cagactgcta cccccgtcac gcagttccag ctggtcaatg gcctcacggt cattgtcaag
     6001 accgaccggc gtgcgcccac tgcggcccac atgctctggg tgcgggtcgg ctccatggac
     6061 gaggtcgatg gcaccaccgg cgtggcccat gtgctggaac acatgatgtt caagggcaca
     6121 cccagcctga agccgggcga gttctcacgc cgtgtcgcgg cgctcggtgg gcgcgaaaac
     6181 gcgttcacat cccgggatgc caccggttat taccagcaaa tcccttccgg caagctcgag
     6241 gaggtgatgc gcctggaggc cgaccggttt gcccacaacc agtgggccga cgaggagttc
     6301 atcaaggaac tcgaggtcgt caaggaggag cgccgcctgc gcaccgaaga ctcgccgcgc
     6361 gcgctgatgc acgaagccgc caacgccatc acctttgttg cgtcgcctta ccggcgcccg
     6421 gtggtgggct ggatgagcga cctcgaggcc atgacgcccg acgatgcccg cgcgttttac
     6481 cggcgctggt atgtgccggc caatgcggcc gtggtgattg ccggcgatgt ggatgtggcg
     6541 caggtgcggc ggctggcgga aaaatactat ggcgccgcgg ttttccgacc ggtgccgtcg
     6601 cgcaagccgc gcatcgagcc ggtgcagacc ggcctgcgca ggatggactt caaggcaccg
     6661 gccagccagg cctatgtgag cctgtcctac aaggtgccgg gtctgcagtc gccagcgaac
     6721 gccgcgccgg ccacggcgga cgtcttcaac cgcgatgcgc tggcgctgac ggtgctggcc
     6781 gctgtgctcg acggttacag cggcgcccgg ctggaacgcg ccctgaccca gggtgacaac
     6841 cggctcgccg acagcgccgg cgcgtccaat ggcctgatcg gccgcggccc gcaggtcttt
     6901 gtgctcgatg gggtgcctgc cgccggaata accaccgacc aggtggccga cgtcctgcgc
     6961 cagcaggtcg cgctgattgc ccgcgaaggc gtcagcgagg ccgaactcca acgcgtcaag
     7021 acccagtggg tggccagcga aacctacaaa ctcgatgcat tgttcagcca ggcccgcggg
     7081 ctgggcgcct actgggtcaa cggcctgccg ctcgacaccg acgagcgcct gatcagccag
     7141 ttgcgcagca tcagcagcgc cgaagtgcag gccgtggcag cacgttattt ccgtgacgac
     7201 caactgaccc tggccaacct gctgccgcag ccgcccgacc cgggccgcgc caaacgtgtg
     7261 cctgccgcgg ggttgcggca ttgagcgcgc gccgcccgca cggggccttc tcttccatcc
     7321 tgtcttcttc gggaaccgtt gtgacaagtg ctatcaaaaa aagagctatt ggcgccctgt
     7381 tgatgggggc tgcagccatt tttcatccca tgttttcatg ggccgcgatt cccgtgcagc
     7441 actggacgca gccctgcggc gccagggtct acctggtcga aagcccggcc atcgccatgg
     7501 tcgatgtgca gctggacttc gatgccggca gccggcgtgt cgcggccgac caggccggtc
     7561 tggccgggat cacc
//
LOCUS       DAIDFG010000145         7571 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_145_length_7571_cov_18.075040, whole genome shotgun sequence.
ACCESSION   DAIDFG010000145 DAIDFG010000000
VERSION     DAIDFG010000145.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7571)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7571
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145_length_7571_cov_18.075040"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1347 a         2370 c         2541 g         1313 t
ORIGIN      
        1 atggcaccgc cttcggtggg cgtggcgatg ccgaggaaaa tcgtgcccag caccaggaaa
       61 atcagcagca ggggcgggat cagcacaaag gtcacacgct cggccatgcg cgacagcagg
      121 cccaggcggg tgaccttgtt gaccacggcg atcacgaagg ccaggatcac acccaggcac
      181 atggacacca cgatggtttc atcgagcggc accgaggtga cggtttcgcc tttccaccag
      241 gtctgcacct cggcgtagtg tttggcaaag tagacggcgc tgcccgcaga ggcgatggtc
      301 agcagcagca gtgacagcag gccggccttg ccgttgtcct cgcggatggt gcgcgcttcg
      361 gccggcaggg cggggaccca ctgcggcttg aagaaggcca gaacaaacac atagccaaca
      421 tacatgccgg tcaggatgaa gccgggcacg aaggcgccct tgtacatgtc gccgacgctg
      481 cggcccagct ggtcggccag gatgatcagc accagcgagg gcgggatgat ttgcgccagg
      541 gtgcccgagg cggcaatcac ccccgaggcg atacgccggt cataaccgta gcgcagcatg
      601 atgggcagtg aaatcaggcc catggagatc accgaagcgg ccaccacgcc ggtggtcgcc
      661 gccagcaggg cgccgaccag gatcaccgca atggccaggc cgccgcgcag cggaccgaag
      721 agctggccga tggtgtccag caggtcctcg gccatgccgc tgcgctccag caccaggccc
      781 atcagcgtga aaaacgggat ggccagcaag gtgtcgttct gcatgatgcc gaacagccgc
      841 agcggcaggg cctgcatcag ggcttctggc aagacgccga gttcaacacc cacaaagccg
      901 aaaaacaggc cgcaggcgcc gaggctgaag gtcaccggaa agcccatcag caggaatacg
      961 atcagcccca tgaacatgat gggggcgagg ttggcgataa aaaattccat gatgatgttc
     1021 ccgatcagcc ggcgctggtt ttggattcgg cgaggcggcg tatcgcctcg gccagctctt
     1081 cttcggcggt cttctcgacc ttcttggcgg tcgggtcttc gatcaggccc ttcaggaaag
     1141 caatgcgctt gatcagctcg gaccagccct gcagcagcag caggccaaaa cccagcggaa
     1201 tcatggcgta caccggccag cggatcagcc cgccagcatt ggccgagact tccccggagc
     1261 ggtagccctg caggaaaaac ggaatgccgt agtacaggat ggtcaggcac acaggcgtca
     1321 ggaaaacaga aaaaccgatg atgtcgatcc agatctgccc gcgtttggac agcttgctgg
     1381 agaccacgtc gatcttgacg tgttcgccgt ggagcagggt gtagcccgcg gcgagcagga
     1441 aggcggcggc aaacaggtac cactgcactt cgaggtaggc gttcgaactc atgttgaagg
     1501 ccttgcggat cacggcattg accgcgctga tgacagtgga ggcaaggatc agccagatca
     1561 cgtatttgcc gatctgggca ttgacccagt ccacggcatt ggaaaacttc agtaaggcgc
     1621 gcattttgtc tccaggaaaa aagaacccac gcataaaaat catccggcag gccttctggt
     1681 ggagggcgtg ccggatactg gttggcgcgg atgggattct atagaggact atagaagcca
     1741 agctgacagc ttcgtgacgg tgttatcccg tagcgaaggg cgtttggcgc ggcggattcg
     1801 accccaaaaa ggtgcgtcgt gcggcgggca ggccgggttc agacgacctg tacgccgctg
     1861 ccctccagca tgcggcctat gacgcgggcg tccgaaaacc cctggccgtg gaagcaggcc
     1921 agaacctcgt tggcgatctc cggtgcgcag gacaccagca gcccgccgct ggtttgcggg
     1981 tcggacagca gggcctgctg cgccgcaccc aggttcgccg gcagttgcac ttcatggcca
     2041 taagcggccc agttgcggcc ggaggcaccc gtgaccatgc cgcgtcctgc cagatccagc
     2101 acgtctggca gcagcggaat gctgtccatc tgcaactcca tcttcaggtt cgcgccgcgc
     2161 gccagctcca gcccatggcc aagcagtccg aatccggtga tgtcggtcag ggcatggaca
     2221 ccctccattg ctgccagggc gatgcctggt ttgttgagct gggtggtgac ggcaatcatg
     2281 cgggcatacc cctgggcgtc gagcgcttct ttcttgagcg ccgccgacag gatgccgacg
     2341 cccagcggct tgcccaggat cagcacatcg ccggcgcggg cgcccgaatt tttcttgacc
     2401 cgcgagggat ggaccaggcc catcaccacc aggccgtaaa tcggctcgac cgaatcaatc
     2461 gtgtggccgc cggcaatcgg aatgccggcg tcgcggcaga catcctggcc gccgcgcagg
     2521 atggcgccta tggtttccag cggcagtacg ttgatcggca tgcccaccag tcccagtgcc
     2581 atgatgggtg tgccgcccat ggcgtagacg tcgctgatcg cgttggtcgc ggcgatgcgg
     2641 ccgaagtcgt agggatcgtc cacgatgggc atgaagaagt cggtggtggc aatcagcgcc
     2701 tgctcgtcgt tgagctggta gaccgcggca tcgtccgcgg tttcgatgcc gaccagcagt
     2761 tgcggcggga tgttgaggcc gggcatgttg ctggagttct tgaggatttc actgagcacg
     2821 cccggggcga tcttgcagcc gcagccgccg ccatgggaca ggctggtcag gcggggaggg
     2881 gcgacaggtg tgagtgggac gggtgcgttc atggggaggg tttcctgggc ggtgcggcag
     2941 acagtgcgct gagcaccaag tttaagacaa ggcgcttttc cgcatcgggg tcgaacaggt
     3001 gggcccgctc gctgcattgt ttttcgagca gggccagaaa acctgcatcg gtggcgcagc
     3061 gcagcaggag cgccgccagc gcagcgtcgt cacccacggg aaagtaaccc gcataagcgg
     3121 cgcccagcat gccgatgttg ccgctgatcc gcgaggccag caccggtgtg ccggactgga
     3181 cggcctgcaa aatgacctgg gcgccgcctt ccatgcgtga gctgttgacc agcacatggg
     3241 cgcgcttgat gtgctggcgg gtctgggcct ggggcaggcc gcccagccag cggtagccgg
     3301 ggacgctggc ctgcgctgtc cgcacggcca gcgcgagatc cggtgtgagt gcgtccccga
     3361 tgtggtcgaa cagcaggcgc accggcgggc gtgagcttcc cggggccagc cgtgctgccg
     3421 cccgcatcca ggtcagcggg tctttttctt cccgcaggtg gccaaccatc agggcgcgaa
     3481 agtgccgcac gggtttgacg gcaggcttca aggcgcgggc cgactggtaa atcacgcggg
     3541 ccttgtggcg cagggtggcg ggcagcgcct gcaggccggc ctcctgcagc accaccaggt
     3601 gggtggccag cgccagcgat tgctgcgcac tggcgtccgc aaggatgtcg cggtacaggt
     3661 cggtgcccgt caaaacgagg atcagcggtt tgccggggca ctgccgcgcc caggcttcga
     3721 tggagggcgc cgagcggcgc gcatgcagcg cgatcagggc atcggccggg gacgtggccg
     3781 gatggggaga ttcaggccac tgcgccccca gcgtggtggc acagtggccg gccaggcatt
     3841 gcgcccagcg cctggctgtg tgccagttgc cattgttggc atcggccagg gccggactga
     3901 ccagcaccac ggtttttgtt ttggacacgc tacccatgaa ccaccttgtt tcgcgagccg
     3961 gagagctccc cgcaacaagg ttaacaggcc tcaggccggc ccgccttcgg catccctgtc
     4021 ggccttgagc tcgcccttga tcgcgtcata gacaaacact tttttggcca gggccggctg
     4081 gtagttgagc atgtcgatca ggaagctcag ggcgccgagg ttgttgccga ggcagtcaaa
     4141 cgttccggcc gcgcgaagct gctgttcgtc agtatcggcc tgcagcatgt ggtcgacgag
     4201 ttcgcgcatg tgcaccaccg ccagcgcggc ctggtccagc cccagcaggg acaacacacc
     4261 gcgcatctgt gccagttccc tgggcaccgc actcaacacc gtcttgtcct ggggtgcgcg
     4321 ggaaaagttg tccagcgatt tttccagacc gcctagcgtg acccgcagtt ctcccaccag
     4381 gctggccatg gtctgtttgt cgctgacacg ccggtaaagc tcttccatcc agggctccag
     4441 gggctgcgac ggcccgccgg cctgggccgc gtccagccgt tcggcgagcc gggccgtgcg
     4501 ggccgtgagc tgggggtcgc tggggtcgag gtcgtcaaaa gcggcttcga ggtacaacac
     4561 ggccgtcgcc acttccagcg ccagttcggg gccgggcacc tggcctgagc gtgccgtcgt
     4621 gtcgatcacc cgtgccagca cgcgcgccag cggctcgctg ggcgggtgca gcttgaccag
     4681 cgagtccgtc accaggctga actggtcgtt catggtcttg aaccgggtga ggtcgcccga
     4741 ggtcacccat gaccagatgt ccttggccga actgatccgc ttgcgggcct gcgcgatcag
     4801 cgccggggca aagcgaccga actggatggc ctcgtagtcg atgggctcga agcggtccag
     4861 gccataactg gcacgcaccg ccgacagcac cggcgcatcc gtggcgcttc tgggcaccgc
     4921 ctgggtacag agaaacagca aatcctgcgc gagcctgtcc gacgcatggg cattgccctt
     4981 tgccagggcc gcatactgca gcaggatgcg caaaatggct cttttggcat acacatcgac
     5041 tttcagcagg cccttggcca gggcttcaaa aaagccggca gccagtttcc agaaggcccg
     5101 gggttcactg tctgtttccg cctgcgcgaa acccaggcac atgtcgcaga gctgttgcgc
     5161 cgcctcggga tggctgttcc gcacgagttt cagggtccac cggtccagca gctcccggct
     5221 ggcgtcgccg tagggcaggg gatcggcgcc ggacgccagc tccggctcaa tccagcgcca
     5281 ctcgaatgcc cagaggtcgg ccggatggat gcggtccgcg ccgaccaggc cctggacatc
     5341 acggtactgg ggaaacagcg acacggccga ggctggtttg cccgcaagca catcttcgag
     5401 gtaagcggtc agggcgaaac cggccagttc gaccttgttg gcggcctccg gggtgcattg
     5461 accgggcgtc tggacaaact tctgcaccgc ggcctccatc gcgcgcagca ccagtgccgg
     5521 cgcactcatg ccgaccatct ccagggcacc gaccgcctgg tgcagctgct ggcgggcgag
     5581 gcgcaactgg ctcgcatcga gcgcggcaag ctcggcgccc tgcgccacct cggcatcccg
     5641 cacaaaacgt ttcagcgcct tgctcgaact gtccagtgat ttgccaagtt catcgagcac
     5701 ccaggccagg ggactcatgt ccatgatgct caggtcgaga tcttcggaag aggcattcag
     5761 ctcggtggat ggcataaatg gcggcccggt tgatgaaggg tgctcacgct cgaaacccaa
     5821 agaggcgacc agcgagctgg aaaaactagc ggagtctatc ccggccgagc cgatctggcg
     5881 atggtcgggg gagggcggag tgacaaccgg tcgcatgagc cggttgactg gttgactccg
     5941 gcccctctcc cgtcggggtt ttttacgggc gacacgggca gatcgacgcc gccccgggca
     6001 gacgcccgtc tgctattctg ggttccccca tgcgcggtgc gccacttgcc agggttggca
     6061 tgctgcagcc ggcttaccag gacgacggtt ttggcttcag atccgctacc cccttcatca
     6121 ccccattcct ctgctgccca cgaggccggc ctgattgatt cgcccctgat acgcagcgcc
     6181 ggacgggatg cgctgtcgct ggccctgatg gatgcgcgca accatacctt gcacctgttc
     6241 ggccagtacc agcaggcgct ggaggccgtc aactttgtgg ttcctgagtt gacggtcctc
     6301 aatccgccgc tctgggagct cgggcatgtt ggctggttcc aggagtggtg gatcgggcgc
     6361 aacatgcagc gcgcccgggg agcccgctgc gagcccgcgc atgcgcggct ggcctccatc
     6421 gaaccgggtg ccgaccgctg gtgggactcg ggcaacgtgc cgcatcacca gcgctggacg
     6481 ctggatcttc ctccggtcgc ggcttgccgt gcctatttgc tcgacacgct cgaaagcacg
     6541 ctggagctgc tggaaaaaac cggggaggac gacgacgcgc tctatttttt ccgcctctgc
     6601 ctgttccacg aggacatgca tgccgaagcc ctgagctaca cggcgcagac tttgggcctg
     6661 accctcgaca aggcgctgct gcaagccttc acgccgccca ccatggccct gcgcgagccg
     6721 ctgttggtgc cggccaccgt ctggatgctc ggcagcgccg cagcgccggg attcgtgttc
     6781 gacaacgagc tggcccagca cgaggtcaac gttcccgagt tcgagatcga cgcccagccg
     6841 gtgagctggg cgcagtttgt ggagtttgtc gatgacggcg gctacgacca gcccgagttc
     6901 tggcagagcg aaggctggga ctggctgcag gccacggcga gcagtgaagg acggcgcggc
     6961 ccgcgttatg tcgagcagat cggtgtggcc agcggcgcgg tgatgcagac ccgctttggc
     7021 aagccgatgc gcatgctggg caaccagccg gccatgcaca tgagctggtg ggaagccgac
     7081 gcctggtgcc gttgggccgg gcgccgcctg ccggccgagg tcgagtggga ggtggcggcg
     7141 cacacggccg cgcggcgagg ttttcgctgg ggcgatgtgt gggagtggac gggcagcacc
     7201 ttccggccct atccgggttt cgagccgcac ccgtatgccg attattcgca gccctggttc
     7261 ggcacgcaca aggtgttgcg cggcggttct ttcgcgaccc gcgcccgtat gaagtctccg
     7321 aagtaccgca acttctaccg gcccgagcgg gacgacatct tttgcgggtt tcggtcctgc
     7381 gctctgtagt gctatctgtt tgatagctgg taacgctttc tgtgtaaggg ttggaggtgt
     7441 atttcatgct aaatccggcg tcacgctgcg cctgaggtct gttctggaca gtaggagcga
     7501 ctccccactc ccacccctaa cccctgccct cgccccgctg ggcgatggag ggggacttca
     7561 tttggcaagg t
//
LOCUS       DAIDFG010000146         7545 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_146_length_7545_cov_20.990921, whole genome shotgun sequence.
ACCESSION   DAIDFG010000146 DAIDFG010000000
VERSION     DAIDFG010000146.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7545)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7545)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7545
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146_length_7545_cov_20.990921"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1135 a         2434 c         2505 g         1471 t
ORIGIN      
        1 gccagcgccg cctcgcgttc atcgagaccg tgcgccgcct tgccgaacag cgtgcggctc
       61 agcgcatcga tgccgacgat ctcgccgcga aacggcacga ggttgaggta ggcctcgagg
      121 atctggtcct tgcgccagct gcgctccagc agctgcgccg acacggtctg gccaatcttc
      181 tgcgcgacgc tgcgcccacc cgctgcggcg cgccagtctt cgtcgagcag cccggccagc
      241 tgcatgctca aggtggaagc gccgcgggtt ctggtggccc acagatttcc ccaggcggcg
      301 gccgagacgg cgcgccagtc cacgccgctg tgttcgtaaa agcgcttgtc ctcgctgagc
      361 accagcgccg tgcgcagcgc cggagaaatg tctggcaacg ccacccactg gccgcgccgc
      421 acggtggcgt cggtgcgcag gcgctggatc acttcgccgt tgcggtccag gatcagcgtg
      481 tcggaaggtt tgaactcgcg ttttacttca ttaaaagtgg ctgtagccca ggcgggccgg
      541 gcgcaagtag ctattaaaaa aatagcaact gcggctgccc tgatggcgga ggcgttcatt
      601 tgaccgcttc caccttgacc cgcgcattcg gcgcttcccc aaacatctcg ggcgcataca
      661 tcgcttccac acgactcggc ggtagcgcga agtcgccgac gttgttcagg cgcacggtgt
      721 attccatctt caccacgccc ttgggcaggt attcgtaata actgcggaag gcctcgaagc
      781 tgcgttcttc aaacgccggc cagcccgagc ccgttttctt ctcaccgctg gtggcgatct
      841 gcgaatcgcg gcccaggccg ctgccgagaa tggtcgcgcc gccgggcacc ggatcgctga
      901 tcaccaccca ggtcatgtcg gtactggcgt tgacttccag cgtgatgcgg atcacgtcac
      961 ctcgtgtgta gaggcccatt ggcaacgatt tattggcttg ctccaccggt gtgatggtct
     1021 ttttgatctg gtagccggcg ttgaacggcg ctttcagtgg gaccgctgcg agcgactgca
     1081 aggtcagcca gggtttgccc gtgccctggt gcgtcacggt cagcgtgtct ttgcctgtgc
     1141 cggcccaggg caaaaacatg ctgttgcccc ggaggttgcc gggtgatgcg ggcgcaccga
     1201 agaaggtggt ctggttgggt gcgccgctgg cgtcggtcgt cttgatgcgt tcgaccttgc
     1261 gccagtccac actggcggtt gctgcggcca gcgcggcctt ggtgataccg gccaccggcg
     1321 tggcctcgaa tttcgccgag aagcggtcga gtgccagccc gccccacaga ttggcggtgg
     1381 tggtgtgcca cgcgccttgc tgctggcgtg ctatgaagcc attggccagg cgacccatgt
     1441 cgtccttcca ggccgcatcg tccatcaccg ccagcatcag acgcgcggtg ttgacgtcgc
     1501 cattggtcat gagccaccac cagtagtcgt ctttttcggt gctgaagctg accttggtgc
     1561 cttgccacga caggcgcgac ttgagcacct ggttcgcctc ggccagacgc tgctcgcgct
     1621 gtggcacgtc ggccacgcgt ttgaggatgt tcagccagtc gatcaccgtg tgggtcggcc
     1681 actgattcgg cgcaatcgtg atgctgccca gcatgcggcc ctgggccttg ccgtaacgcg
     1741 acagcgcctc gatcgccgcg agcttgcgca tgtcgaggtc cttgcgcggg ctccagaact
     1801 cgcgttggat gcggccctcg acaaacgcaa tcaggccgcg ttccatcggt gcgcgggcgt
     1861 cgtcgggcag ggcgaacgcc ggattcaggg ccgcggcttc gtgcgtggcc gccagcaggt
     1921 aggccgtcaa ggtgtcgctg ccgcgcgagg cgtcgccgtc acgcggcggg aaataactgg
     1981 ccaggccgtc gcggtcgagg taacccggta gctgcgccag cacggtctgc cagagtctgg
     2041 cgtcgcgcag gccgacggcc ttgctggttt tctgctccag gcagacgaac gggtagttgg
     2101 cccaccagtc gcgcacgcca tccagaccct ctgcgagttt cggttgcagc gacatcttca
     2161 aacctccgcg gccgggcagg gcatcggccg gcgggttcac gctgagcatg aacgaaccat
     2221 cgacctgcac cagcgtggct tgctgcaccg tgagcggcac agcggggatg atgcgctggc
     2281 gtgccttgag gccgtcacgc gcgccgctga tgttgtcctt cgcctcgatt tcccacagga
     2341 tggcttccgc gcgtgtcagc gccagttgcg ccggcgccgt gaccatccac gccacttcac
     2401 gcgcttcgcc ggcgggaatg tcgacggttt gcggtttgag ttcgagcaag gtcgcacgcg
     2461 gactgacctc gaccttcatc gccgcctggg tcgtgttgcg cagggtcagt tgcgcgcgga
     2521 actggtcgtc ctcgcgcacc agcggcggca ggccgctgat gatctgcagg tcctgcgtgg
     2581 cccggatgct ggtctggccg gtgccgaaga ggccggtggc tgcatctgcc accgcgacga
     2641 tcctgaaagt ggtcagcgca tcattgagcg gcaccgtgac gacggcctgg cctttggcgt
     2701 ctagcatgac cttggggttc cacagcagca aggtgtccag caactcgcgt gtgttgctgc
     2761 gcccgccgcc gccgcccgcc ggcacggcct tgcgcccgta gtgcctgcgg ccgatgattt
     2821 ccatttgtgc ggtcgaggtt tccacgcccc aggcgcggcg ttgcagcatg gcttccagca
     2881 ggccccagga ggtgttgggc atcagctcca gcagtgcctg gtccaccgcg gccagcgcca
     2941 cctcggcgtt ggcggccggc tggccattgg gcagtttggc ggtgatggtg acctgggcct
     3001 gcccgcgcac cgggtagctc tccttgtcgg ccttcacgct cacctcaagc tggtgtgcct
     3061 tggtgcccac gcggatttcg gccacgccca ggcggaaggc tggtttgctg aggtccacca
     3121 gcgcggtggg ggccacatac tcgcggccct cgtaccagaa cgaggtccac cattcgcgcg
     3181 gcgccttgaa accccaggtg aaaaagctgt accacggcac ttcgcgcagc cggccgcgca
     3241 gggccagcac actgacgtac acgttcgggc cccagccgtc cttgacctgc agctggatgg
     3301 ttgggtcctg gccgttgagc tgcaccacct gggtctcgag gatgccttcg cgctcgatcg
     3361 ccaccagcgc ggtggcaaaa cggaaaggca tgcgcacctg aaacttggcg gtctcgccgg
     3421 gctggtaagt tttcttttcg ggcagcacgt ccatgcggtc gtggtcctcg ccgccgaacc
     3481 acagctcgcc ctgcttggtc acatacaccg agctggcggc ctgaatggtg ttgccgtcct
     3541 tgtccttcgc ggtcacgacc agttccactt cgccgggctc gccgagtctg gcctcgcaca
     3601 gcagcaggcc gcgcgcatcg ctggggccgc tgcagacaga gccgaggtcc ttgatgtcgg
     3661 tcttgttgtc gtaggtgtag aagccgccca ccatgcgttt gcggctggtg gtgacgatgc
     3721 gcgccaccgc cttcacctcc aggggcacgc cggccgccgg tttgcccgtc aggtccagcg
     3781 ccagggcctg gaacctgatc ttctggccgg tggacaccca gccctcggtc ttgatgccgg
     3841 cgaccacggc ggccggccag agcgtggaga cgctgcgaat cgtctggacc tcgccgttcg
     3901 ggtcggcgta gctggcttcg agcagcaact ccctgggctg cttgctggcc ggcacgtcct
     3961 ggatggtgag cttgccggca ccgttgcggt ccaggctcag gggcagcttg tcggcaatca
     4021 cgcgctggtc ttgcgcggcc gtggcttcct cctcgccttc gctgccttcc ccgcgccggg
     4081 cgcgcggcgg cgagaaactg aaggcctcgt gatcgctgta gctcaaggtt ttgccgcgta
     4141 ccagtgccga cacgcgcacc ggcaggttga cggcggcacc gccagagaca tagttgacct
     4201 gcacatcggt gggcaccgag cccacgttga ccagggcttt tttgtcggcc ggggtgatgc
     4261 ggccttcgag caccggcagg cggaactctt ccacgcggaa ttgcccgctg ctgaagctgc
     4321 ggtttttttc gtcgttggcc ttgagttgca cctcgtagat gccgagtttg gccgcgggcg
     4381 gaatcacaaa ggtgttttcc gcgctctggc cgccggtggc ggtttttcgc cagacgatgg
     4441 gctgggtgta ctgctggccg ctgccgatat gggtcaccac cagggtggct ggcggcgctt
     4501 cgacgatgcc aaacccgcgg ctggtttcgg tgcgcaggat gtgtttcatc gacacgattt
     4561 cgccggcgcg cagcagtgtg cggtcgaaaa tcgtgtgggc gcgcgcatcg ggctgcgggt
     4621 cctggctggt gggcagattg aagcgccagg gctcgatgcc tttttgccag ttgctccagg
     4681 tgaaggccaa gtcttcaata cctcccgagg tggcgcgcgc gctcacaaag taggcgttgc
     4741 cgccgtagtc gccttcgccg cagcgcagcg ggtccgacga caggccggtg aagcgggcca
     4801 tgccttgtgc gtcggtgacg gacttggcca gctcggtccc ccggcagtcc gacacgcgca
     4861 ccacggcgcc ggcgaccggc gcgcccttgt ccagcgtggt cacccaggcc agcgcgtttt
     4921 cgcggcccag cttgaaatgc acgcccatgt tggtcaccag cgccgaggtg cgcacaacca
     4981 tggtccggct gtcaccgtgg cgttcgtcga gcagcgaggt accgagtttt tgcgaggcga
     5041 tttcgaccac gtgaaaaccg ggcgagagcg ggatgccgat cacctcgaac ggacgcgggt
     5101 cgccgctcgc aggcttgggc agatccagcg tttttacgcc gggttggccg gccagcagcg
     5161 agagcatgcg cgactgcacg tactccttgc tttgtgcgtc gagggccttg ggcagcggtc
     5221 ccttcacgtc ccggccggcc tggtcgcggt ccacctggta gtcgttgtaa tgctccacct
     5281 tgcggaacca ggcaatgatg tcggcatcgg ttttgggctg caggtcgctg accttgccgg
     5341 gggacaggcc tttgacctgt agcgcggctt ccacattgcg cagcgtcacg ggcagcagcg
     5401 ccgggtcacc cgctctggcg ccgggttcgg caaaccgctc gacgatgcca aacggtgcgg
     5461 cagcaaactt ggccagcggc ggcatggcac cggtagccac cttcaggggg aagttgtcgg
     5521 cattgcgcag gctgcggccg gacgcgtcct tgaagtcctt gggaagctcc agcgtgaagg
     5581 cggtctgctc tgcaaaaggc ggcttgaagc tgacaccgtt gaccacgttg tcgccgtcgc
     5641 tgctctccgc gtcgaacacc ggcttgagcg tgtttttggc agatgtgagg cgaatcgcct
     5701 cggccagctt gcgcggcacc ggcgcgttga agttcaggct catgggtcgt atcggcaggc
     5761 aggccgactg ggcgttttcg cgctcgcagt tgaagctggc agaaaacggc tcgcgcacct
     5821 ggaagttgta gcgccgctcc accgagttgg ccacgccgct cggagtggcg acaccttttc
     5881 cgtacaccag ctggagcttg ctcgcgggtg tgaggcggcg gttgcaggcc agtgtcacca
     5941 cgctcaaggg gtcttttccc gccgccttgt ccaggttttg cgacttgagc agcgcatccc
     6001 gctccttgcc ttcgatcagt ttcacgggaa cccgctcgcc caggccctcg accgagcacc
     6061 agacgttttc gcggatgctg gccagggtgg ccgggccgtt gagctggagc acgaagtact
     6121 gttcttcgtc gatgcgttgg taagtgccgg gccgcaggtt ccgtacgaaa ggaccgccgg
     6181 tgttgaactt ccacgacacc ttgccgctga tggcctggtc tttgggcgaa cggaaggtgg
     6241 tgatgccttg cagcgtgcaa gtgatgccgg gcggaagatc gttgtcgaat tcaaacgccc
     6301 actggcggtc gctgatccag cggcccgaac ctttggtggc ctgcgcgtcg ctgcagctca
     6361 ggctgacagg ggcgggggct ttggggtcgc cgaagttgac ggcgctctcg tcaaacttga
     6421 cggcaatctg ccgtaccctg gcaatttccc cctgaggcga gacgctggtg acctgcaggg
     6481 cctgcgcgct ggcgggcagg tacaaagcag cggctgcggc cagtgcttcg gctgttcttc
     6541 ttatggtgct tgtggccacg gttcctcctc gttgggaggt caagcgtagc tgatttgcat
     6601 gtaacaaaac agcgcaaacg catggaataa aggcgcaaga aaaaagcggg ctgcggcccg
     6661 cttttgccat caacagcgct tgaaaatctc agctgagttt gaccggtaca aaaatcttgc
     6721 tgtcgccacg ctgtatcagc agtgccaccg atttctccgc tttggccaca gtggcacgca
     6781 cctgctcaat gtttttcacg ggaactccat tgatggagag caacacatca ccggcctcca
     6841 cgccggccaa ggcggcggga ccgctggcct gctgcaccag caggccgctg tccacaccgg
     6901 cttcctgttg ctcgtcgggc tgcaggggcc gcagcgccag gcccagcttg ccctgggcga
     6961 cgctgtcttt cgggcctgca gcctgggcgg ccttctgatc ggcgctgccg agccgggccg
     7021 tcaattcttt cgccgcgccc tggcgccaga tctccagctt gaagctgtcg ccgggcgctg
     7081 ccaggccgat caccgccggc aggtcgccgg aggacacgat gggctggccg ttgaccttgc
     7141 ggatcacgtc gcccgattgc aggcccgcct tgtcggccgg cccgcctttt tcaacgctgg
     7201 agacaagggc tccctcgggt ctgtcgagct tgaaggagtc ggcaaaggcc tggttgactt
     7261 cctgcacggc cacgcccaga cgcgcatgct cgaccttgcc ggtggccacg atctggttct
     7321 tgatgcgccc cgccacgtcg atgggaatgg caaaggacac gccctggtag ccgccgctgc
     7381 ggctgtagat ctgggaattg atgcccacca cctcgccgcg cgcattgaac agcgggccgc
     7441 ccgagttgcc gggattgatc gccacgtcgg tctggataaa gggcacgctg ctgtcgtcgg
     7501 gcagcgaacg gcccttggca ctgaccacgc cggccgtcac cgtgt
//
LOCUS       DAIDFG010000147         7504 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_147_length_7504_cov_18.924285, whole genome shotgun sequence.
ACCESSION   DAIDFG010000147 DAIDFG010000000
VERSION     DAIDFG010000147.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7504)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7504)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7504
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147_length_7504_cov_18.924285"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1420 a         2448 c         2351 g         1285 t
ORIGIN      
        1 gcgcagcgcc ccctcggggg gcagcgcagt acgcgaagcg acaagcgtgg gggctctacc
       61 tcgggggctt taatagtcag gctgccagtc caccgtgatg ctcttgcccg gcagatcgac
      121 ggcatccaca taggcggcca caaaggggat catgcgctcc agtatctgct cgccttcggt
      181 gtattcgaca cacagcaccg aatgcggtcc ggtggccatc aggtcgcgca ccttgcccaa
      241 caccacacct tcgcggttga cgacgttcag gcccagcagg tcgacccagt aatactcgtc
      301 cttgccggcc ttgggaaaac tgctgcgcgg cacaaaaatg cgcgcgccgc gcagggcctc
      361 ggccggcgta cggtcgtcga tgccctcgaa cttggccacc acggagtcgg aatgggtttt
      421 ggattcgctg accgtcagca ccacggtacc ggagaaagcg tcaaaaccgg gccggtgttt
      481 ggtatccggc ggctgcagaa accagcggcc ggaagaaaaa agcgcctcgg aagaggcgct
      541 gtgggccagg attttcaccc agcccttgac gccccaggcg tcaagaatgc gcccgacttc
      601 aaccgcatcg tctggcaggc cagacggctc aaggccgggc aacattcagg aacccgacaa
      661 tcaggcagcc ttgggagctg cagcagcgcc ctgcttgacc aggcgcagca cggtggggga
      721 cgcttgtgcg cccacgccga gccagtgggt caggcggtcc tgctcgatac gcaggccttc
      781 ttcgccacct ttggcaatcg ggttgtaaaa accgatgcgt tcgatgaaac ggccatcgcg
      841 gcggacgcgt ttgtcagcca caacaatatt gaaaaatgga cggtgcttag aaccgccgcg
      901 cgagagtcgg atgacgacca tgatttatcc ttcgggtggt gaggtgaaaa aaaatcaccc
      961 caaaatgttc ctgcagcgct tgagacacac gacatggcac ccggccagcg aaatgctgca
     1021 aagccgcaga ttataacccg cggcctgccg ctgtcccgtc cgctgcgttt ccccaacgtc
     1081 cttccaaccc atttttggca cgccatgacc ctcctgagag ccagccttga caccgatttg
     1141 ccggccatca ccgccattta cgcccaccat gtgctgggcg gcaccggcac ctttgaaacc
     1201 gaaccgccca gcgtgacgga catggccacg cgccgtgccg acgtgttgtc caagggactg
     1261 ccctacctgg tcgccgaagt gggcggcgag gtggtcggct ttgcctacgg caactggttc
     1321 aagccccggc cggcctaccg ctattcggtg gaggattcga tttacctggc acccggccag
     1381 cagggcaaag ggctaggccg cgccctgctg accgaactgc tggcgcgctg cgaggccgcc
     1441 ggcattcgca agatcatggc catcatcggc gattctgcca acgcgggatc ggtcggcctg
     1501 caccgcgcgc tcggcttcac gctggtgggc gtcgtcgagt cctgcggctg gaagatgggc
     1561 gcctggcgtg atattgtgat catgcagaaa acactgggcg ccggcgacac ttcgccaccc
     1621 ctggataccg cgcccccctc ttcctcaccc tcccgcccct gattgcattc atgaaaaaca
     1681 agaacaagac actggccgcc tggctggctt ttgccggcgg ccagctgggc atccatcgcc
     1741 tctacctgta cggatggcgc gatctgccgg cctggctgca tcccctggcc gctgccctgg
     1801 gctggtgggg cgttcagcgc gtgcggcagt acggccagga cgaccagctg gcgtgggcgc
     1861 tggtaccgct gctgggtttc accctggcgg ccacggcgct gacggcgatt tattacggcc
     1921 tgatggcccc cgaaaaatgg aatgccaaac acaacccgtc ggttgcaccg gacagtgctt
     1981 gcggcaaaac caactggatc accatggcgg ccgtggtcct ggccctgctg ttcggcacga
     2041 ttgcgctgat gtccagcatc gtgttcagct tccagcgcta ctttgaatac caggttgaag
     2101 aagcgcgcaa gatcagccaa aagaggtagc gcccccacgg ttgctcactg cgtgtagcgc
     2161 cctgcccccc gagggggccg ctgcgcctgc ggactggcaa agccagatcc gcggcccctg
     2221 cttgacgaag atcgcccgcg cgctaaatcc ggacgtgatc agaaaacctg caggctgacc
     2281 cagtaggcga tggccgcgac aaaggccgaa gccgggatcg tgaaaaccca ggcccagacg
     2341 atgttgccgg ccacgcccca gcgcaccgcg gaggcgcgct gggtggaacc caccccgacg
     2401 atggcgccgg tgatggtgtg ggttgtcgac accggaatgc ccagaccggt ggccagaaac
     2461 agcgtgatgg cgccgccggt ttccgcacag aagccgccca cgggcttgag cttggtgatt
     2521 ttctggccca tggttttcac gatgcgccag ccgccgaaca tggtgcccgc ggcaatcgcg
     2581 acgtagcagc tgacaatcgc ccaggttggc ggggcgctgt cgctggccga ggtgtagccg
     2641 gtggcaatca gcagcatcca gatgatgcca atggttttct gcgcatcgtt gccgccatgg
     2701 cccaggctgt aggcgccggc cgagaccagc tgcaggcgcc ggaaccagcg gtccacgcgc
     2761 gacggcgtgg cgcggcggca gacccaggcg accagcacca tcatcagcga gccgagcaga
     2821 aaacccagca acggcgagac aaaaatgaag gccacggttt tcaggatgcc gccggccacc
     2881 aatgccccgg cgcccgactt ggccatcacc gcgccgacga tcccgccaat cagcgcatgc
     2941 gaggaactgc tgggaatgcc gtagtaccag gtcaccaggt tccagctgat ggcgcctatc
     3001 agtgcgccga acaccacatg ggtgtcgaca atacccggct gcacaatgcc cttgcccacg
     3061 gtggcggcca cactgaggtg aaagacaaag atggcgacaa aattgaaggt cgctgcaaac
     3121 agcaccgcct gcccgggctt gaggaccccg gtcgacacca cggtggcaat cgagttggcc
     3181 gcatcatgaa agccattcat gaagtcgaag gccaccgcca gaatgaccag catgaccacc
     3241 acccacaggg ccgtctgtac ggtctccatg ttcaggagtt ttcgaggacg atgccctcga
     3301 tcaggttggc cacgtcttcg catttgtccg tgatggtttc cagcagctcg taaatcgcct
     3361 tgagcttgat gacttcgcgc acatcaggct cttcgcgaaa cagcttgctc atggccgagc
     3421 gcatgacgcg gtcggcatcg gactcgagct tgtcgatttc ctcgcaggtc ttcagggcgg
     3481 cttcggccgt ggcggcgtcg ctgagcctgc cgatcagggc gaccgcgtct ttcaggcgct
     3541 cgcagcattt gacgctgagg tccgtcaggc gcacgatctc ctcggtcatg tggtgcacgt
     3601 cgtacagcgc catggtttcg gccgagtcct ggatcaggtc gcagacatcg tccatggtgt
     3661 tgatcagcga atggatctgc tcgcggtcga tcggcgtgat gaaggtcttg tgcagcatgc
     3721 ggttgacctc ctgcgtgatc cggtcggccg cgcgttcggc gttgtcgact tcccggttgt
     3781 aggcttcgcg cttttgcaca tcgctgtagt tggccaccag gttggagaac gcgcgggcag
     3841 cctccacgat gcggtcagca tgctggttga acaactcgaa aaaattgcct tcacgcggca
     3901 acagtttgcc aaacagcatc ttcaactcct cgggggtgca tgtcagttat atatgacaga
     3961 tttgtgacta tcgggagttt aacgccccca cggtcgcgca ctacgtgtcg ctccctgccc
     4021 ccccgagggg gccgctgcgc ctgcggactg gcaaagccag atccgcggcc cctgcctggc
     4081 taagatccgc tcgctcccaa ggccctgggt caggccgacc ggaagatgaa gtacaccgcg
     4141 cccaccatgc acagcgccgc ccacaggtaa tcgagcttga gcggctcgtt gaggtagaac
     4201 accgcgaagg ggacaaacac cgtcagggtg atgacctcct gcatgatctt gagctgcccg
     4261 atgttgtaca gcgtgaagcc gatgcggttg ccaggcacct gcagcaggta ctcgaacagc
     4321 gcaatgcccc agctcaccag cgcggcgatg taccagggcg cggtcgccag gttcttcagg
     4381 tggccgtacc acgcaaaggt catgaacacg ttggacagca gcagcaaccc gatggcttgt
     4441 gccgagatcg gaagggattg cagaaattgc ataaaactcc gtgttgagtc gttgggccgc
     4501 cccgaggtca ctccaggtca ggcagcagca gaaacaccag gcttgagagg tttttaatgg
     4561 ctccagccct tatccagcct gctcgaccag ctataaaacc catagcagtc aacaacacca
     4621 ccggaaacag ggcggcaaac acaggtgaat tcatgcggcg atcttagccg cgtgccctac
     4681 acgcgacggg cgccctgccc ctcaggcccg ggcaatccag cgctggtggg cggcgagttt
     4741 ttccacatcg cgccaggact ggcactgtcc ggccatgcgc ggccagatgc gcagcagcag
     4801 ctcgcactcg gccagtgtgt cggccgccgc ctggtgccgc ggcgcgcaga tgatgccgaa
     4861 atgagccagc cattcgtcga gtgcgcgcgc cttgaccctc tcatgggtca cggcgcacag
     4921 ctgctcaatg tccacccagc ggttcggcag ccccgcgccc aggtgctgca ggacatgccg
     4981 gccaatcatg gcctggtcga acggagcgtg aaaggccagc agcggcgagt cgccgatgaa
     5041 gtccgcaaag gcgcgcaggg cctggtcggg cggcacccct tcactctggc gctgcagccc
     5101 gatgccatgc agcaggatgt tgtctttgct tgatccgacc gaagactggc gcagcaccac
     5161 ctcgaaactg tcgcccggca tgatgctcag gcgtctggcc gtccagtcca cctgcacggc
     5221 aatggcggca atcgccagca actggtcgcg ctggggatcc agcccgctgg tctccacatc
     5281 aagcaccacc cagcgcgcct catccgcgcc ggccagcgcg gcgaaaggct ttttcagcca
     5341 gtccgcccag ctcatcgttc gtagtccaac tgcaggcgct gctgcagcga gcgcgccacg
     5401 cgaaacgact cgcgcaggat gcgcctgtcg atgtcgttca gacggtcgac tttcaggtga
     5461 ttcggctgct gcggcgaggc cgtgccttcg agctgcacgc gcaaccgcag ggtctgcaaa
     5521 aattcgaaac cgctgaccca ggcctggtat tcggacgggg ccagccccag cagcggacca
     5581 acggcgtcca gccgttcccg ggtgttggtg gccgtcacgc cgcggctgag cgaataaatg
     5641 cgcgcgacat cgacaaaaat cgcggcgccc tgcagcttga ggtcaatcat gccgtcgtcg
     5701 tccaccgcaa tggagccggt ccagttcagc ggcgggctgt gcatcagcgc gttggtcgcc
     5761 agttgcttga tgaagcgcgg cgcgcgccgc gccgactcgc tgatctcctg gcgcaggctg
     5821 tcggccaggc gcgcatcgcc ggccagcggc cggaagtcga agtaaatgct cgcattgagc
     5881 aaatcctcgg gcgccccgtg ttctatccag tgcgcaaaac gctcacgcca ctgatgcagg
     5941 gtcaggcagc aggccggctc gctggccatg atgccgccgc ggcacagcgg gtagccgcag
     6001 gcatcgagcg ccacattgac ctcacgggca aaacgcagcg cggcctcgcg ctgggccggc
     6061 gtcgtgtcgt caggcaggat cagcgcgttg tcctggtcgg tcgcaatggt ctgctcgctg
     6121 cgcccttccg agcccagcgc caaccagcac aggcggctca gatcgatgcc gtgctccccg
     6181 gccttgatct ccagcaggcg caaggtgagc acgtcgttga ggtggctgat cagcgccgtg
     6241 agctggcgcg catgcacccc ctggcccagc aggctgtgcg caaaccgccg gatgtcctgc
     6301 gccacgatct tgagcatctc cacatcggcg gcagaacgga tcgaggtgct gacctgcttg
     6361 aggctcaggc gctgcatcgc aaacaggtcg cgctccgaca ccacacccac ggcgatcccg
     6421 tcacgcgtga ccggtacatg gcggatgccg tgtttgctca tcaggagggc cgcgtccagc
     6481 gccgtgtgtt cgtggctcag cgtcagcacc ggctgcacca tggcctgcgc gatgggcgtg
     6541 tccagcggca gctccgccag cgtgacacgg cccaggatgt cataccgcgt gagaatgccc
     6601 agcggccgcc cgccctcgtc ggtcaccagc atggagccga tgcgctgctg gtgcatgcgg
     6661 ctcagcgcct cgcgcagcgg tgtgccgggc ggacaggtca ccggcgcctg gcgcgcgaca
     6721 tcgcccagcg gtgtctccag cgactgttcg gccagcgcct gcgaggcata agccacctgc
     6781 agggcgcgcc gcgagaggtc caggaacttg aggatgcgcc ggttcaggaa gtcggcaaac
     6841 ggcgggctca tcacggccag cgcatgcatg gaagccagcg gcagggccag cacaaaggtg
     6901 tcggccacgg cgcggtagga cgccgtcacc gcgcgctgcg ccagcgctgc actgacggga
     6961 aacagctcgc ccggttcgta gtggaaagca ccgcccgaca gctcggccag cccgcgctcg
     7021 ccgatcaccg cgccctggcg gatgaagaac agttccgtga ccggcccgct gtcgggctcg
     7081 atcaaaattt cttcgggtgc gaagtagttt tgccgggcat ggctcaagaa gaagtccacg
     7141 tcgccggcct gcatctgcgc aaacggcgga tgccgcatca gttcctggcg caaattggcc
     7201 agcaggctgc tcgacgggct caagtcggcg ttgagcaggg aaagaggctg ttcggcatcc
     7261 gtcacgggcg tctcctgata ttttgaaaac cattatccgg gaaggacttg ctgttgtgct
     7321 gattcagcgc aaacggggcc accgttttgc cccaatccca ctcctgcccg ttcgggctga
     7381 gcctgtcgaa gtctgtgcaa gcttgcgctt cgatgggtgt tcaaacaagc cgggtctcgg
     7441 cccggcggcc gactcacttt ctcttgcgtc gccaagagaa agtaagcaaa gagaaggcga
     7501 cccg
//
LOCUS       DAIDFG010000148         7498 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_148_length_7498_cov_9.669488, whole genome shotgun sequence.
ACCESSION   DAIDFG010000148 DAIDFG010000000
VERSION     DAIDFG010000148.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7498)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7498)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7498
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148_length_7498_cov_9.669488"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1257 a         2227 c         2332 g         1682 t
ORIGIN      
        1 gtaaactctt tgagggccat tcaatcactc catgatgtag cgcttcccgt caggcccctg
       61 gtaaaccggc ttcccgccag acgtgccaat ttgcttgtgg tccttgggga cagaagcctt
      121 gatttttggc gctgcagcgg ctgggctggg acttttgttc atgccgcgcc gcgctaggcg
      181 cttctcaact tcggcggcaa tctccgcctc gctggaatac ccgccaagct ctttctcgac
      241 ggccgcgatg atggcgttgc gggtcttggc attggtggcc gcctccccgg tgcccttgct
      301 cggtgccacg ccgatggaga cgggcgctcc cttggtcggc agggttgtca cggtcgcttg
      361 gccggtgtct tcgtcaacga tggtcacggg cttgtccagt ccggcaatca cacgatccag
      421 gcgcgcagtg gacaggccgg cggacgcgcc tgcagaaccc gcctgggcgt tgttttcgtt
      481 gaccttggac tgctcaacgg tctggaaaat cttcgccaac actgggttga tttctccgcc
      541 cgtgccggtg gccttgttga tggaatagcc ggtgctgccg acgttgtcaa aaggcaagta
      601 aggcttatct gccgtgacag cgataagctt gttcatggcc tcgatattgg gctctgcaac
      661 agcccggtcc cggaagcctt gggcctggaa ctctgtccca gccttggcaa cccgttcggc
      721 gttagtgtcg cccgtcatct tgaaaacccg gcgcaggttg cgctcatagg cctgcatgga
      781 tggatcggcg gcaatctccg cgtccatgcc ccggcgctgg tccagggtga agttttcctg
      841 cgcggcctgc gcgccgtact tgttgccggc ctgggtgtgg gagtaggcgg tggccgcctt
      901 ggctgcggct tcctgttcgg cctgctgccg gtacattgga cccagcgcat acgccttgaa
      961 caggctgccg atgccctgcc ccgccgcttg gccgcctgaa ccgtcaagtg tgaatttcat
     1021 cgtgttaccc cagtatcttg aaggtagcgc tggcgggtat ccggaagccc cctgtggccg
     1081 ccccgttcat ttttaggccc atgcccccgg tttcgttgaa caagctggtg ccggcgccgg
     1141 tagacccctg gagtcctgac ccggcggctt tgcccgcaga tcccgcgccg gccatcatcc
     1201 cggcggagcc tgcgctttgc aggagcgagc cggcaaacat catgccgggg tccacaagcc
     1261 cggcttgctg ggctgcgatc tggtcggccc cggcctgtcc gcgcgagaac cctgcaaggc
     1321 ggtcgatgtc catcccggtg tctgccaggc ggataccttc gttcatgcgc aggcgattgg
     1381 ccgatgaggt cttccccatc atgcgggcca ggctctcggc cgccttcatg gcgttgatgt
     1441 cgctggcggc cttggcggtc gtgtagtcgc tggagacgtt gccctgcgtg gtctgctggc
     1501 cggcgcggat cgtctgactt tcgctgactg gcgcaagcag ttcctgcgtg atggtgtctt
     1561 cgatctgctg ctgctcggcc ttgcgctctg gggtagaaaa cttttcggcc gccccgagtg
     1621 cctttttctc ggcctgcatt tgaagctcac gctgcgaagc aagcccggcc cgaatttcag
     1681 cctgctggcg cttttgggcg tcggcactcg ctttgtactg cacggccgcg ccggcaacca
     1741 tggccgccag tgcggcgatg gtggttccgc ttattgcacc tgccatgtca ttctccggta
     1801 atcaaagttt ttacgtcggc ctgccggttg gtcatcagca gctcgtagtc gtcggtaaat
     1861 tcgcgctcgg cctcggccac ggtcttggcc ttggttggga agctcatgct catgctgaca
     1921 tcggtgtggg caagaaacgc ctgaatgcgc ccggcgcttc cgggcaatac cgaatagccc
     1981 cgcagctcga tggtttcctc tccgatgaac acagcgcagt ccccggacac aaccaggacc
     2041 gtgggcacct tgagcagcgc gccggtaaga acctggtccg ccttcatgtg aagggtgcgg
     2101 atgtacatgc cggcatggat taaatgctct gtcgggaatt cgacttgctc gcacagcgca
     2161 agggaaactt cctgcaggct gcgaaccttg gcaatggcgt cctcagtcat ggccggcagg
     2221 cgctgggcct gggccatcag gccggagcct gggcggactg ctatcgcggt gggttcttgc
     2281 atggccgtcc tgccttactg ggttttgccg gagtaagtcg ttccgggcgc tgaaacgcca
     2341 agccactggg cacctggtag ctgtgcgccg gccgtgcgtc cgagccctgt ctgattcatc
     2401 aaataggctt ggctcatgtc gttgaacagg ctgccgacat tggccccggc gcgctgcgaa
     2461 gctactgaat cggcgttgac cttgaggcca ttcagcgcca tcgaggccgc agagccggta
     2521 tcaatgccgg attgagccat gctgattaga tttgcgcgcg tcttttcgtc agcggctcga
     2581 aggtccgcgg cggactggtc ggcgatgcct ccggcgcgca gcagtccttc gttggttcgg
     2641 cggttgatgt ctcccgcgct ttctacgtcc acggaaccgc ccgccaaccc agcacgggcg
     2701 agcccgaagc ggttggcgcg ctccgcgtcc tgggcctgtc gggtgacttc cttgctgttg
     2761 aggtcataaa cggcgctacg ctgatccttg tagaggtctt cgcgcggatt gaactcggct
     2821 ccaggcgtga agatgctgtt gattttctcg gtcgcggccg tgatgcgcgc ttggcgttcg
     2881 gcctcttggg cgcgagcatc agcgccgggg tcgccgccgc cgcccttatg gtagagacgg
     2941 acgccgtcgc gggtgtagcc gctatgtttg tttgtaatca gcatgatgta gctccgttta
     3001 aatctttgtc cgcaccactt ggtacacgtt ttcaaattcg taccggcgca gcagccgggc
     3061 cattgcattg ctgcatgagg cttcaatcac cgttgccccc gcatccctgc accattcttt
     3121 gaaggtgccc caaaactgtt ctgccgcctc atcgacaaac ccgccgccca gcgccaggat
     3181 gttcacggct agggtttgcg ggtagtggac aaactcaaat gcgccggcca gtaccgccac
     3241 accgtcacgc tcgaccactg ctgtgataac ccggccctcc agggtcaaac gtgcgatgtc
     3301 gtcaaccgtg aactccccgc gcgccgcctc tttcactacg gtttctaaca atggcgcggc
     3361 catgtggaat ttttgagcga aaagctccgg gctgacgata aatgtcacgt tcataccggc
     3421 cccagatcgt tgaaataaat cgtcacggaa tcgagctgga agggctggtt gtcgtaattg
     3481 cggaagcgca gggcaaactc ggtgccggag cactcaaccg ggatcgtgcc ggcgggcctg
     3541 gtgttgccct tgacgcgcac ggcgttggtg aaggcgtccg gatcgcgcga atcccagcca
     3601 acagagaact cggcccggcc agtaatgaca acgtcagcgc cgacaatctg cttgaggttg
     3661 ccgggcagct tgaaatccat gtaaggcagc tcgatcagca cttcgtattg gctaccgtcg
     3721 tcggtgctca cgctatcgtc cagcttgtaa acggcgtccc cggaccggat gtaaagggtc
     3781 tggcccagct cggcgaatgc gtcaaccggc acgctcagaa aatactcgct ccaggcggcg
     3841 atttttgctg tgcgtgaaat cgaatacaca aacaggcggt tggcaatcgc gcaaatgtat
     3901 tgcccggtgc cgtagaaata gaaggacttg ggggccacgc ctgggacttt ggtttcaggg
     3961 cgcaccagtg agtcgatggg cgagccaaca tccacgtccg ccaggttgct ggtcagttgc
     4021 agcgtcgtga tggaccggaa gccatagtct gaaaggaagt gcagatcgcc ggccacgctc
     4081 gccaccgtgc gcgggaaact tgtcccgacg ttttccacgg ccgtgtccag cttcatgttg
     4141 gtggggtctg ggtccacaat ccacacctgc gcgccgtcct tggtcagcac cacaagcttg
     4201 ctctggtagc tgcccagcgc attggcagat cggtcgccgc ggctattcag tccggtaggc
     4261 aggaagccag catcgccggc aagtgtccag ttacgcgggt cgctggtttt gcagtagcgc
     4321 accgtgtcac cgatgccgct gccgacggca aaaatcttgc ttgccagctt gatgcaggcc
     4381 ttggtatcgg ggcaattggc gtcaacgatg tgggttgctg gcgtgccgtc aaggtagtgg
     4441 tgctcgatct ggccggctgc gtactcgatg gacgcgtaaa tgaaggcgtt gaagacatcc
     4501 gcaaagtgaa cgtctttgac tgcctgagct ccgccggaat aaacaacctt gttgggattg
     4561 aatagggtgt tggcgtgcgt taccgtgcct tcaccgtaaa acgtctgcag cttgccgaac
     4621 gcggcaaaca agcccttggt gccgggttcg aggtcggcaa ccttgaccag gccggggcgc
     4681 ttctgtgtcg ccaggccggt ggtcacaaag gcgtttttca tctggcgcag gcggttggcg
     4741 tcagaaacag aggccccctt gcgcaggtca atgcccaggt cgaagcggtc gaaggtgatt
     4801 ttgctcacga cgcgcccagt ttgtagccgt cagcggtgcg gacaacttgc aggctgccgg
     4861 atccggccgg gctgccgttg acaaaatacc tgcggttttc gcgctgccgg cccttctcac
     4921 gggacagcat gttttgaaac gccgtagcgg atgcctgggc gtctgggtgc cggtagtggg
     4981 ccttggccgt cgccagggcg tacaggaaca ccaggcggtc cggcacgctg cagcggtcgg
     5041 cggtctgggt gaagcgggcc cgctcggcga tgtattccac cgtgatttca taggcgcgct
     5101 cgggcagcgg ccatatttcc atctgcccgt ttagggtgtc gtacttgcgc ggctgttcgc
     5161 gctgctcgcc gtcggcgcgg tcgtagttcg tgatgccctg cgtcatctgc tgggcttcgg
     5221 tgttgcctac cttgatgaag acggacagca cgcggccggg gttgatgtct tcatcatcgg
     5281 catcgttgtg ccagtcgtac aggtaagacc cggcctgggt gctgatgatg gcctttttgc
     5341 gctgtaccgg cggctccagc tccccgaaaa catactcatg ggattccgtc aggaagctct
     5401 tgatgatggg atcgttggcc ttggccgcgg agccctgaga ttgaaagccc aggcgcgcgc
     5461 gcagctcgga catcaattcg cccaaggttc ggttgcgtgc ggatagtcca gtcatggcct
     5521 gcctttacgt ggtggtcaga gaaatggtca actcggtgcc gaccagaatc aggcgcgcgg
     5581 atacctgcgt ccggccgata aggctggccg tcttggtggc ggtgtcgata gtgacaatcg
     5641 caccgtctgc gccgtcgcta ccggcggctc ctgttggtcc ttgaatacct tggatgccct
     5701 gcaggccctg gatgccctgc ggacccgttg cgccggttgc acccgtggca ccagtcgcgc
     5761 caaccccaaa ggtgtacgcg ccggaccagt cggcagaggt ggcggacagc ttgaaataaa
     5821 gattgccggt gtccagggca aagaagctga aaccctttgg ctgcaggttg tacagcgcgc
     5881 ggttggcaaa cagatcccga acgtcggcgt cgaaggatgc gccggtatcg cccttcactc
     5941 cctgaatgcc ttgcgggcct gttggaccga ctgggccggt gggtccggtt gggccaggca
     6001 cttgcagcgc agccaaggcc tcggccgtca aagacgtggc gttgactgtg ttgttcttaa
     6061 gcgtgccgtc atcgttctgc agtagagcgg cgttgtcgcg cagcccgtcc accgataggg
     6121 cgacggcgtc caactcggaa tttatcgcgc cgtggtccgt cgccgtgctg gcgttctcaa
     6181 gaaaattctt ggtccggttg taggctggtg cttgcatgga tgccctttac tttcgcgcct
     6241 gctcttgcag ggcttttact gagctgtcga tggtttccac gcggaacttc aagaggttga
     6301 cttcgctgtt gagcaccgtc acggactgat tcccgctttt catcaggatt tgcaattcct
     6361 caacggcctt ctgcgtttgc agtccgttga agtacatgcc gacaagcgca agcaggcccg
     6421 caccgaccac actcagcaca ccccagaggg gaacgctgta gtcgatagtt accctcgtgc
     6481 tggtgatggt gtgcggctcg gtcatctgaa atttccttat cggtgaatgc cgtcggcgtg
     6541 tttggtgtcg taccacttga ggtaatcgtt ctggatccgc gcgcagcggt cgaagcgctt
     6601 gccgctgccg gctgcagcgt gccggttcag gcgggcggta acaatcaatt ccttgggcca
     6661 ttcctgcagg tagatggtca tccacacaac attgacgatt aggtcaaaca catagccgcg
     6721 tagcagcatg taggtgccta ggatcgtggc tggctttgtc aggccctgcc cggggatgtc
     6781 gcgcacgcgg gccaggttca tcaaggccca atagtgttca atcaggctca acggtgaagt
     6841 cagcaccagc agcacaaaaa ggaacagttc aatccagaga gtgatttcaa gaatgctcat
     6901 ggttcaacct cgatggttcg gatttcggcc tccgcgtcct tgcactcgcg gtaggtctta
     6961 ttgcgggcat aaatgcccat aattgccagc tcaagcgtta cgccgtattg ggttgccagc
     7021 aaaggcgcgg ctaccatttc ggcaaagctg attacttgct ggatcagcgt gcggtccttg
     7081 cctttgccct gagtcgccaa gacttgcgcg atgcgctgct cttttgtgat tggtacgtag
     7141 ggcggctgcg gcgcttcatc ccgctccttg gccgctgcgt gcagggcgat gtagacaccc
     7201 atcgcaacct catcactgat ggttttttgt gggccgttgg tgtactcgat agtccccgct
     7261 gatccatacc actggattgc gtgaacgtct gacgggtatg tagccgccgt tggtttccgc
     7321 aggtcaccat ccacaatgat gacgttatcg acagggacga cggtgactct cacagcttga
     7381 tgatcttgtt agagacgtac gtgggttgcg tgttgttgtg cgcagcgctc ccgcccgtgt
     7441 atcccgttcc gcccacaccg gccgttgcgc cttctggcga tccgccacct acaggacg
//
LOCUS       DAIDFG010000149         7477 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_149_length_7477_cov_18.483428, whole genome shotgun sequence.
ACCESSION   DAIDFG010000149 DAIDFG010000000
VERSION     DAIDFG010000149.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7477)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7477)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7477
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149_length_7477_cov_18.483428"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1257 a         2633 c         2361 g         1226 t
ORIGIN      
        1 cccgctccag gccgccagtt cgcgcccggc cagggcctgc aggtccgcca gcaccacgtg
       61 gcgctgcagc atcagcgacc ccgcctcggc cacggcgggg tcgctgcgcg agacgccata
      121 aggggtgggc gttccccagg cattgatgac ccgtttcagg tccggaagaa caggtgttgt
      181 gaagcttggc atgaagaaaa ttccggaatt gtgcgcccgc atgcaaattt cttcaaaccg
      241 ggcgctccgc aaaccgctag catcaagggc ttccaaggag actccatgcc cgtgatcacc
      301 accatcgaag acctgcgcgt tctggccaaa aagcgcgtgc cccgcatgtt ctacgactac
      361 gccgactccg gctcgtggac cgagagcacc taccgcgcca atgaaagcga cttccagtcc
      421 atcaagctgc gccagcgcgt ggccgtcaac atggaaaacc gcagcacggc cacgcagatg
      481 attggccaga acgtggccat gccggtggcg attgcgccta ccggcctgac cggcatgcag
      541 catgccgacg gcgagatcct gggcgcacgt gcggcgaaaa agttcggcat tccgttcacg
      601 ctctcgacca tgagcatctg ctccatcgag gacgtggcgg ccgggaccga tcgccatccc
      661 ttctggttcc agctctatgt gatgaaggac cgcgacttca tcgagcgcct gatcgaccgc
      721 gccaaggccg ccaactgctc ggcgctggtg ctcacgctgg acctgcagat cctcggccag
      781 cgccacaagg acctcaagaa cggcctgagt gcgccgccca aaccgacact ggccaccatc
      841 ctgaacctga tgaccaagcc gcgctggggc ctgggcatgc tgggcaccaa acgccacggc
      901 ttcggcaaca tcgtcggcca cgtcaagggt gtggaaaaca tgggttcgct ttccgagtgg
      961 accgccaagc agttcgaccc gggcctgaac tggggcgacg tggaatggat caagaaacgc
     1021 tggggcggca agctgatcct caagggcatc caggacatgg aagacgcgaa actcgcggtg
     1081 aactcgggcg ccgatgcgct gatcgtttcc aaccacggcg ggcgccagct cgacggggcc
     1141 gagtcgtcga ttcgcgcact gccgcagatt gtggacgccg tcggcagcca gatcgagatc
     1201 cacatggacg gcggcatccg cagcggccag gacgtgctca aggcacgggc gctgggcgcc
     1261 aagggcacct acatcgggcg ctccttcctg tacggcctgg gcgccatggg ggaggaaggc
     1321 gtgagcaagg cgctgcagat catccaccgc gagctggacc tgaccatggc gttttgcggc
     1381 cacaccaaca tcgagacggt gaacaagggg attttgctac ccggcactta ctgacagcgg
     1441 cagccagtcg gagttggccg ccagactcag gtccagcagg ggctcggcgg catcccacca
     1501 ggcctgcgcg tctatgtgcc ctgcgagatc acagtccagg tagaccagcc cgccgcggtg
     1561 cgccatcttc tgcagcgcgg cgtgatcgca aaacggctgt cccagtccta ccaccacggg
     1621 ccggtgcacc agacgcgcac aggccaccac cagccgctgc atcaggtcgc cgtcacgggt
     1681 gctgtcgccg ctgagaagca ggatcacgtg ggaatagtcg ggcagaaagg cctgcgtcaa
     1741 tccgagatgc aggccattgc gtgccatggc gatcacccgc cgcacgacgg cgccggaatc
     1801 gagatccaca taatcggtcg ccacgtccag cccgtcggcg cggctgccgg cgaagcgccg
     1861 gtgcaccacc tcggccttgt gcgtcctgcc cagccagcgc accatgtcga aggaagcctc
     1921 acccctggcc gcacagacca tgacgcgctg gaaccggtcg ctgaccgcct gttcagccgc
     1981 cccgggatgc gtcagcggtg tggacaggcg ccgccacacc gtcgcaggac caggcgcgcg
     2041 gccctgttga tgctcctcgc gcagtgtgac cacccgccgg gcgaaatcgg ccggcagctc
     2101 gagcgcctgc acgatggcca tggaccaggg ctgcgtcagc cggcgcgggc cgcggctcga
     2161 atgcagcaag cgcaggccgc ggtgacgcgt gtcgcgtgcg acgcgcccca tcagggcatg
     2221 cacacccgca ggcggccctt ccatccattc aacgaaccag ccgctctggt gaagaagcgc
     2281 cacatgaacc tcgtcagcca cattgcgttg caaggcgtgt tcgcgaatgc gctgcatttc
     2341 ctgaaacacc gggccggtga aattgctctg gctggcgtac atgaggcggc ctacctccct
     2401 gtccgtcgcc gttgccgcgc tatccggcca atcactcgtc atcaaatcca gcaccacaag
     2461 cccttcatct ctcgcctctc atctctcacg tatgcacccg ctacaggcaa cgccacatca
     2521 tccccgaaag gccaccgcac cgtattgcgc cgggtcaagc ccgccccaaa tccagcccgc
     2581 cagatgatca atggacggtc gccctgttgg ggaatatccc catgacagtg cgctgccaaa
     2641 gaaataagct tgcttatcaa ttacccgggg tgctccaggc gcttcgcttt ccccatgacc
     2701 tctccccagg aacccgccat ccacacgccc ccggtgcgcc gcttcaagga cccggactat
     2761 gtcgaggtcg ccccgacact ggccgcgctg cgggagcgca tcgacacgct ggacgggcag
     2821 atcgtcgagc tgctcgcaca gcgcgcgctg tgtgtgcgtg acgccacccg tttcaagcgc
     2881 gacgccttcc aggtggccgc gccggcgcgc caggccgaag tcttcgcgcg cgtgcgggcc
     2941 cttgccgcac accacgaagc cgctttcccc ggactgtccg acatcgtcga gtccacctac
     3001 cgcacccttg tcgccggcta catcgcgggc gagggcactt tttttcacga cacggaactg
     3061 atcaagccat gaacaccacc ctgccccgct ttccactcac acacctgctg gcacgctgtg
     3121 ccgtggcggc caccgttgcg cttgcagcaa gcagcgccat ggcccaggcc gcttcgacag
     3181 gctcaggaca ggcctggccg aacaagccgg tacgcatcgt ggtgccttac ggcgcaggct
     3241 cgtcgccgga tgtgatcgcg cgcatcgtgg ccgacaagct ctcgccgcgc ctgggccagc
     3301 cggtgatcat cgaaaaccgc gccggcgccg gtggcaacaa cggcaccggc gccgtggcca
     3361 aatcggcagg tgacggctac aacttcgtca tcagcaccaa cgggccgctg gtgtacaaca
     3421 ccgtgctcta caagaagctg ccttatgacc cgttcaccga gctgcgcccg gtggtgctgg
     3481 ccggcggcca ggccaatgtg tgcgcggtac gcgccgactc gggcatcacc acgctcaagg
     3541 atctggtcga cgcgatgaag aaaaatcccg gcaagttcaa cttctcatcg accggcgtgg
     3601 gcagcctgtc gcagctcggt gtggagctgc tcaaggtcaa gaccgacacc ttcgcggtcc
     3661 acattccgta tgcctcttcg ccgctggcca tcctggccat cctgcagggc gacgtgcagt
     3721 ttgcctgcgt gcccgcggtg gcggtgatcc cccaggtcaa ggcgggcaag atgcgcgctc
     3781 tggccgtgtc caccgccaaa cgcagccagc tcatgcccga catcccgacc atgaaggaag
     3841 ccggcctgcc cgagatcgaa aatctcgcct ggatggccgt gatggccccg gccagcacgc
     3901 cgaccgagat cgtgcggcgc atgaaccagg aaatcaacgc cgtgctcgcc atgcccgagg
     3961 tcagggaaaa actccacgcc cagtacatgg aacccatagg cggctccgag caggacctgc
     4021 agaagttcat gcagcaggaa ttgcgcgtga tgacccccat catcaagcgc accggcatct
     4081 ccgtcgagta gcgccgcagt gcgcatgatc ggcctgcgcg tccagcgcag cgcggccacc
     4141 ttggcagagg tcgacgcggc gctggcgcgg ctggagaccg aggccggcat ttatttcggc
     4201 gtcgatgccg gcattgccgg cctgcatccg ctgcaggcca cgctgctggt cgaacccgca
     4261 ctggccctgc gtctgtatgg cgacgggctg gaagtcgagg ccctcaccgt ttttggccaa
     4321 gacctgctgg cgcagcccgg gctggcccgc tggcgcgctc agaccgtgcg ggggccgcag
     4381 gtctcaggca tcacggccct gcgcgctttc ctggcgtgtt ttgatccttc tgccgacgtg
     4441 ctgcttctgg gcgccctgcc gtttgcggcc caccgcctga ccgaacccgg caacgatgcc
     4501 ggcgcgctgg gtgtgctttt tttcggccag cacctgcttc ggcgggatgg cgcaggcgac
     4561 tggcaacacg tcgccttgag ccttcccggg tccctgttgc cggcctcacc gccgaccgct
     4621 tcgctggcag cgcccatcgc ctcccggtct gccctcgatc cggtcgcagc cgaaccccgt
     4681 gacgattttc cggtgggcgc ttacgccgac atggtgcgcc gcgcgctgga atgcctgcgc
     4741 gcgcgtccgc tggtgtcgct gaccctgagc cagagctacc gccgccgcgt gcaactgccg
     4801 gccgtgacgg ccttcgggcg cctgcgcgag gccaatcccg cgccggccag tttttttgtc
     4861 aacgacggtt cgggcttgcg cctgttcggc gcctcacccg acctgcaact ggtcgtcacc
     4921 aatcgcatcg tccagaccct tccggtctgc ggcacggtgg cacgccggcc cggcgccatc
     4981 ggcgaggccg agtcgctgcg cgagctgctc aatgaagagg tggacgccgc gtcgcttgcc
     5041 gtctgcaccg acgcgctgcg caatgatctg gcgccgctgt gcgcgcccgg ctcgctgcgc
     5101 ctgctggacc ggcgccggcc gatgtcgctg gcgaccgtgg tgcatgcggt ggaccggctc
     5161 gaaggacgtc tgcgcgacgg cgccgatgcc tgggacgcca tcgtggccac ggcggcgtcg
     5221 gtgatggtca ccggcacccc gcgggccgag gcgctgcggg ccatcgatga actcgaggcc
     5281 agcccgcgcg gctggtacgg cggcctggtg gtgcaggtgg catccaacgg cgatgccctt
     5341 gccggcacca tcctgcgcgc cgccgcggtt cggggtagcg tggcggaggt gcgcaccggc
     5401 ggggacctga tggccgactc gtcgccggaa cgcgaggagc aggaatcccg gctcaagaca
     5461 ctttcgctgt ggcgcgcttt cgggctggaa gctgcagccg cacaagctgc tgcgcaacaa
     5521 gacatcgccc tgcccgtcgc cgttcacctg gtggatgccg gcgatccctt tggtcatgcc
     5581 atgcgcgact gcctgctggg cctgggcctc ggcctggatc tcgcgccgca agcgccgtgt
     5641 tgcctgcttg tcggggcgaa cgacctggcg gcttgtcctg agcagcacct ggtcgctgtc
     5701 ggtgatgcgg ccctgcgcct gctcggtgca tcgggctgga ccgtgttgcc cgaccggccc
     5761 gaacatggcc gcacgctgct gtgcagcacc accgctgcag cgccctggcg cagcgacggc
     5821 ccttttgcgg cagcgcgtta tgccagcgcc gtgctgcagg ccgacgacct gaaggccggc
     5881 tggcaggctt gggcgctgga cgagcagggg cagccggtgg ccctggccca tgccggccgg
     5941 cgcgtggtct gcctgctgtt tcgccctgac tccctgctct ccagcccgca ggcgctgcag
     6001 ctgctgcgtg cggccctggc ttttgcagcc gttaccgaac agctttagga cctaacgctg
     6061 cccaaaaggc cgcgccggca cggttttcag gtaggcgtac agcgcctggg catccacatc
     6121 gttgagttcg cgcagtgact caaaaggcat cacggtgacg actgcgccgt ccggccgctt
     6181 gccgctgcgc agcatggcga caaactggcc ggcatccttg taacgcgtca tcgcgctgcc
     6241 atcacccggc gtgaggttgg cagccggcgc ccagtcgggc ggggcaccgg gaatcttgcc
     6301 gccggaaagc cccgccccgt gacagctgat gcacatgttg gccacatagg caccgtgcgc
     6361 caccgtcacg ccttcggcca cggcggtggc cggcggcagg gaatggtcga tcttctgcgc
     6421 cgcgtcttca atcgcatcga agccatacag cagcttgacc ggcagcgaca actccagcac
     6481 cgttgcgccg cccggctgcg gcggcaggct gcgcacatag gccaccagcg caccgaggtc
     6541 ggcatcggtg aagcggttgt agtcttcgct gggcatgaca aacagcggcc ggccatcggg
     6601 cttgacgccg tggcgcagcg tacgcaccca gtcttcggat gtgtagcgcg ccaccacctt
     6661 gccctcaccc ggcgtgatgt tcgggcccgc cagacgcaag ccatggccgt cgttgacgaa
     6721 ctcgcggcca gcgccgttga cgccatggca gtccacacaa ccgcgcgaat tgaacaggta
     6781 tttgccgcgc tccagcgact gcgcatcggc cggaaaagcc acgggccgca cattcacctt
     6841 gacgaggcgc tgcgcttttt gatcagccag ctccgtcccc accagcgtcg caccggccgc
     6901 cagcaccacc agggcggccc ctgcgcccag aacccacttg acccatcgtt tcattgtttt
     6961 tcctctggtt tgatccctgt ccacacccgt ttgggacaaa ggcgaaattc tagaaagcag
     7021 gcgctgcctt gtcacgctgg aagccgggca ataagtcaca gcaacccctg ttggcgaagg
     7081 caactattta catccgctgc gtctggcgaa tgccggacca ccgccctggc gggcgggaac
     7141 agcggggcgc cccgttcaac gctatgcttg ccgggtgagc gcctctctcc cgtccaaccc
     7201 ttccagcccg tccagaatcc gccgcattgc gcacctcgac atggatgcct tttttgcatc
     7261 catcgagctg ctgcgttacc cgcagctcaa gggcctgccg gtggtcatag gcggcggccg
     7321 ccgcaaggtg gacgaagcgc tgatggccgc gcagggcgaa cgtgcgttgc gcttcatccc
     7381 ggtcgcggag ttcccgctgc tcaaggacta tgtgggccgc ggcgtcatca ccaccgcgac
     7441 ctatgccgca cgccagttcg gcgtgggctc ggccatg
//
LOCUS       DAIDFG010000150         7452 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_150_length_7452_cov_18.556171, whole genome shotgun sequence.
ACCESSION   DAIDFG010000150 DAIDFG010000000
VERSION     DAIDFG010000150.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7452)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7452)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7452
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150_length_7452_cov_18.556171"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1361 a         2429 c         2400 g         1262 t
ORIGIN      
        1 ccttggcttc ggaggccaat actctatcca ctgagctaca gccgcaacgc tgaaaacatg
       61 gcatctaccg gcgctttgca gcggatagac aacccgcaag ggcagacgct gggcgcactg
      121 cacttgtgga agagcctgat tctatcagtc ggacaggccg cttccgggat ttacaggggt
      181 gacggctata atcaaaggtt gtcgaatatg agttgcttta gttaccgatt tttagttttt
      241 tgaggaagcc atgagcgcaa acgatccaac gacggcccac gaagaagacc actcaggccc
      301 catcaaaaca cccaagcagc tcctgcttgc cgttttcttc tcttttgtag tccctgtttt
      361 tgccatcatt gcgctggttt attacgtctc gtcggacaac aaacctgccg cgggtgctgg
      421 tgacctcgag aaggcggtgg cgcagcgcat ccagaaaatc ggcatggttg aaatccgcga
      481 tgccaaccgt ccgctgaaaa ccggcgccga agtgtatgca gcgcaatgtg ccgcctgcca
      541 cgccaccggg gccgccggcg cacccaagtt tggcgacgca gcagcctggg caccacgcat
      601 caaaaccggc ttcgaagcgc tgtggaattc ctccctcaaa ggcaaaaacg ccatgggccc
      661 tcagggcggc ggcgattttg acgatgtcga aattggcaag gccgtggtgc acatggccaa
      721 tgctgccggc gcaaaattcg ccgagccgca aaaggcggca gatgcaggcg ctgcagcgcc
      781 ggtcgctcaa gcggctcctg ttgccgccgc tcccgcagca gcggttgctc ctgcagcgcc
      841 cgtggtggtg gccgctactg caccggctgc agcgtccaaa gccggtgtcg gcgaagcgtt
      901 gtacaagcag gcctgcattg cctgccatgc gtcaggtgta gccggtgctc ccaagcttgg
      961 cgacaaggcc gcctgggcgc cgcgcatcca gaccggtctg gacatgctga cggccagcgt
     1021 gatcaaaggt aaaaatgcca tgccgcccaa gggaggctcc tctgcctccg acgcggatct
     1081 ccgggcggcc gtcgaataca tggtcagtgc cgccaaataa gcggtatccg ctctaccaaa
     1141 aaaaccggtc tctgaccggt ttttttatgg tcaaaacagg ctcaagcccc tgaggtacaa
     1201 gcgcagacag ctagcaaatc aatagcacta aacaggctgg tcgcggctgg cggccgggct
     1261 gcgcacataa ccgaaacggg acggcaagtc ccgggtggcc gcctccccag gcacccacag
     1321 gccctgctcg atcgcctccg acgcctgcat catgtcctgg gtgtgcacca ggccaaaacc
     1381 aatgtcggtt tccagataga gccggccgtg ttcatcgaga atgcagcgct gcaagcgcgc
     1441 gggccggccg gtgtgcgcgg tcactgaaaa atccggggcg ctgccgatac gccagaccag
     1501 cggtgtcgcc tcgagttcga cataaacgcg ttgcggcccg ttctggaaaa accactgacc
     1561 ggccgcgtcg ctttcgtagt tgcgggcgat gaagtcgatc agcttgtcgt gtttgagcag
     1621 cgaacccttg cccgctgccg tgccgccggc aaaggggccc gccgcctggg ccttgtcatc
     1681 gcgcatgtac cagttgccgc gcgcgtccag acccagccag ccgtagcagt gcgggacgtt
     1741 gggccacttg gcaatggcct ggcgaacaat gtcatccatg ctgcttattc tgccccgctt
     1801 gttgtggcgc cgagatgcgt cgagaaaaac ccgcacaccg cctcgggcat ggcgcgcaca
     1861 tgccccggaa aaggcgccga cgggaaaccg acgtggccac cctcgcgcgg ctgccagagc
     1921 gtcacggctg cactcacgtc accggcctgc ggcaggctgg cggccgggat gaaggggtca
     1981 ttgcgggcat tgagcgccag cgccgcgacc gcgatgcgcc gcatgtgcgg tttggccgag
     2041 gcgcgcgccc agtattcttc ggtgttcctg aacccatgca gcggcgccgt gacgatattg
     2101 tcgaaggcat acaggtcgcg cgccgcgcgc agggcgtcgg cactgaacag gcccgggtgc
     2161 tgctccagct tcctgagcgc cttgggcacc atggagttca ggaacatgcg ggtgtagacc
     2221 aggcggttga aaccccggcc aatcgcatgg ccgctggccg cgaggtccag cggggagcag
     2281 accgaggcga cggcgtccac cacacggtct gccgaagttt ccatttcgcc cgcccagcgc
     2341 atcagggcat tgccgcccag agaaatgccg acggcaaaca gcggaccggc atggccggcc
     2401 cggaagcgcc gcaggatcca gtcgatctcc tcgaaatcgc ccgaatggta ggcacgcggc
     2461 gcgcggttga gctcgcccga gcagccgcga aaatgcggca cggcgcaggc ccagccgcgc
     2521 acgcgggcga catcggcaaa tgcttccgca tagtggctgg ccgaagaccc ttccaggccg
     2581 tgaaaaacca ccagcagggg ttcgcgccct gccgcggcgc ccgcacccgc gctggcatgg
     2641 cgtgcgaagt ccacatcgac aaaatcatca tctggcgtgg tccagcgctc gcggcgaaag
     2701 ctcggcgcct cggccaggta acgctgcgag cgcagcgcgg cataaatggt ctgcaggttg
     2761 ccgccgggca gccaccatgg ggcgtcgtaa aacatcgcgt cttccgcctc agtgcagcac
     2821 ttgcgggctg tcggagattt cctgcatctc gcggggcgtg ccggggctgg catggtgggc
     2881 caccaggcgc caaccctgcg ccgtcttgtg gtacacgttg gtggcgatca cataagcatg
     2941 gcgcgggccc tcttcggtca tgacctcgat gcgctcgagc acgctgtgca cgctggcgcc
     3001 catggcttcg atcttgcgga ccttttcggg atgcgcccga atgctgccgt tggcaaacat
     3061 ggcgtcaaag gcagcgcgta tcgcacccgc gcccacaagc cgcgctccgc cggggtgaac
     3121 acagacaatg tcgtcttcgt cggcccagca ggccatcagc cgctcgatgt cgccgccctg
     3181 cagcgcctcg taaaaagccg tctcgatctc atcggcattg ccgctggtgg ccggggaaac
     3241 tttgggtttt cgcatgggta gataggggga tgtgtatgtt cagtccgccg gggagggttg
     3301 ccctgtatta agccacgccc gcaccacgtc ctgcatgcgg ggcgcctgca cggcctgcca
     3361 caacagttcg ggcgccacca catggatgcc cggcggcagc acccgcagga ccacatccac
     3421 aaactcggcc agcccggcgg gacgcaccgg ttgcgctgcg gacgggatca tgtagctgag
     3481 ggcggacaac atccaggcgg cgaccttttc gggcagactg ccgccccgcg gcgccagctg
     3541 cttttgcgca gaacgcacca gcagcaccct ttcgaacccc agcgcggcca cctgctgttc
     3601 gtcaatgctg gccaggccgt ggcgcagcgc ctgcggcagc cggcctggcg cgtggggcaa
     3661 caccagcacc agggtctgga cgccacaacg cctgagccac tgcgccaacg cgggcagctg
     3721 ggccggctgc ggcgtccaga gcgcgcgctc ccggtcatgg tacagccggg gcggctcgaa
     3781 catcaccacc ccggtctgca ccggcagcgg tcgcaccggc caggcagcga tgtcctcccc
     3841 gcgcacggcc gttatcgaga cctgcgccag cccggtgctc atgggctcgt gggcgagcac
     3901 gtgggtgtgc gcaaagccgc cgctgccggc cagccggcgc agcacctcgc tgcccagcgc
     3961 gccggtggca cccgccacga tcaggcccgg ccgggccgca gccgcggcgg acaccggccg
     4021 caaagatgcc tgcaacacat gccgcggatc aggggcctgg agggaataaa tgagagggct
     4081 gagagggtgc ggcgacattc gactatggta gtgttgcagc ttgcactttt ggaggcttgc
     4141 catgaaatca ctttttttca gccggggcct tgccggcctt ttcaccgctc tggccctgct
     4201 gctgtcgggc tgcggctata acgatttcca gcgcctggac gagcagacca agtcggcctg
     4261 gtcggaagtc ctcaaccagt accagcgccg cgccgatctg gtgcccaaca tcgttgccac
     4321 cgtcaagggt gaggcgaatt tcgagcagga aaccctgacc aaggtcgtgg aagcccgagc
     4381 caaagccacc tccatgcagg tgacgccgga gaccctgaac aaccccgaag cctttgcgaa
     4441 attccaggcg gcgcagggcg agctcagttc tgccctgtcg cgcctgctgg tggtgacgga
     4501 aaactacccc aacctcaagg ccaaccaggg ctttcaggac ctgcgcgttc aactcgaggg
     4561 tacggaaaac cgcatcacgg tggcgcgcaa ccgctacatc caggcggtgc aggcctacaa
     4621 cgtgctcgca cgcagctttc cgaacaacct gacggccatg gtgttcggct acacggtcaa
     4681 acccaacttc agcgtgcaaa acgaggcaca gatctcgacc ccgccgaccg tggacttcaa
     4741 caagaaataa tcggcccagc gccttgcctg tcgtgtcccg ccctttgttt tgcctcccgg
     4801 gccgggccgc ggtcgcctcg gtagcgcgcg cgatgccggg cctgttgctg gccgccggcc
     4861 tgctgttcag cgtcctgaca acgtgggcgc aggatgtact gcccgtgccg gcgctgacgg
     4921 cgcgggtgat ggacagcacc gggacgctcg atgccgcgca gcgccaggcg ctggaggcca
     4981 aactcgcggc tttcgagcag gcccgcggcg cgcaggtggt ggtgctgatg gtgccgacca
     5041 ccgcaccgga agacatcgcc gcctatgcgc agcgagtcgg cgacagctgg aagatcggac
     5101 gcaaggacgt cggcgacgga ctgttgctcg tggtggcgaa aaacgaccgc aaggtgcgca
     5161 tcgaaaccac caagacgctg gagggtgcga tccccgacct ggcggcgcgc cagatcatcg
     5221 accgtgccat cacgccacgg ttccggcagg gcgacttcgc cggcgggctc gacgcggcca
     5281 tcgaccagat catcgccctg atcaaggggg aaaacctgcc cgtgcccgat gcacaggcag
     5341 gcagcccggg cggcaagggt ttcaactgga ccgacatcgc ggtgttcctg ttttttgcgg
     5401 ttccgatagg ggcccggctg atgtcgggca tcttcggacg caagctgggc gccctggtga
     5461 ccggcagcgc ggtcggcggc ctggcctggt ttttcaccag cagccttctg attggtggcg
     5521 tcgccacgct gatcggtgcg gtgtttgccc tgatcgccag cgtgggctcg ctgggctccg
     5581 ccgggcgcgg gggacgatcc tctggctggg gcggtggcgg ctttggcggt ggcagcagcg
     5641 gcggctgggg tggcggcggt ggaggcggct tcagcagcgg tggcggtggt gatttcggcg
     5701 gcggcggcgc atcggggagt tggtgaacat catgatcagc aagctcaagg tcctgatccg
     5761 gcacctgtgg ctggatgcct ccgacacggc gcgagccatc ccgcccgacc tgctggaccg
     5821 gctggcgcgg cgcgtggccg ccagcgagcg gcgccacagc ggagagatcc gcatctgtgt
     5881 cgagtcttca ctgcccgcca gctacctgtg gcggctgggc agtaacaccc ccatcaatgc
     5941 cctcatacgc gaacgcgcag tgatgatgtt tggcaaattg cgcgtctggg acaccgagta
     6001 caacaacggc gtgctgatct atgtgctgct ggccgaacac gccattgaaa tcgtggccga
     6061 ccgcagcgtg gccaggcatg tgagcccggc gcagtggcag gccatggtgg cccacatggc
     6121 cagtgcgttt cgggaaaagc gttatgaaga cgggctgacc caggcgctgg aagaaacctc
     6181 ggcggtgctg atgcagcact ttgctgtgag cgcaggccct gacggcgccg agcccggagc
     6241 caacgaactg cccgacaccc ccctgctgcg ctaagccggg ggcactcacg gcaccatgag
     6301 cacgcccaga gcggcgacca cagccaccag cacgagaagc cgcttgagcc acacgtggcg
     6361 ccaacgtctg gccttgcgcc cgggctccaa ctcttccgcc cacgtcaaac tgtccagaat
     6421 gctgtccatt ggtgtcgcct tgtcttccgg ttgaactcct gggagcctgt cgggcttggg
     6481 aatcgtcgac gccccaagcc cgacaggctc ctggagacaa taacgcccgc cggcgcaccc
     6541 tgctggccct tctcgtgtcc agctgttaca gcaactcctg atttgatagc tgttcgcgcc
     6601 cgccagacaa ggggaaagca tcaatttggc ataaaaaatg gctccgcagg tgcagcgcat
     6661 ccgcaacagc cggccgcgcg gcgccggacc tgggcccgga caggaaacgg gtcgcgccca
     6721 tgaaaaaagc cccggcctga cgggcggggc tttttggtgg ggcgattccg cttatttttt
     6781 ctggcggaac ttgcgcaggg ccgcaatctg cgcggccatc acggccagct cggactgggc
     6841 cattgcgata tcaatatcgg ttttggcatt tttcagcgcc tcctcggcgg cggctttggc
     6901 ggcggtggcc ttggcttcgt cgaggtcctt gccgcggatg gcggtgtcgg acagcaccgt
     6961 gacacagtgg ggctgcactt cgagcaggcc gccggccacg aaaacaaact cttccgaacc
     7021 atcggccttt tcaatacgca cagcaccggg cttgatccgg gtgatcagcg gcgtgtgacg
     7081 tgggtagatc cccagctcgc ccgcctcgcc cggcaaagcc acgaatttgg cctcgccgga
     7141 aaagatggac tcttccgcgc tgacaacatc gacatggatg gtgtgtgagg tgtccatgac
     7201 ttagaccttc ttggccttct cgaacgcctc gtcaatcgtg ccgaccatgt agaaagcctg
     7261 ctctggcagg tggtcacact cgccggccac aatcatcttg aagccgcgaa tggtttcagc
     7321 cagggtcacg tacttgccgg gcgaaccggt aaacacttca gccacgtgga atggctgcga
     7381 caggaaacgc tggatcttgc gggcgcgggc cacggccagc ttgtcttcag gcgccagttc
     7441 gtccatgccc ag
//
LOCUS       DAIDFG010000151         7420 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_151_length_7420_cov_19.584250, whole genome shotgun sequence.
ACCESSION   DAIDFG010000151 DAIDFG010000000
VERSION     DAIDFG010000151.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7420)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7420)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7420
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151_length_7420_cov_19.584250"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1464 a         2304 c         2219 g         1433 t
ORIGIN      
        1 cttcatgaac ttctcggtgc gcaactcgcg cgtcaccggt ccgaagatgc gggtgccgat
       61 aggctccagc ttggcattaa gcaacacggc tgcgttgcca tcgaatttga cgagtgaacc
      121 atcgccgcga cgaatgccct tggcagtacg aaccaccaca gcactgtaga cctcgccttt
      181 tttgacgcgg ccacgcggag cggcttcttt gatactcacc ttgatgacat caccgacgct
      241 ggcatagcgg cgcttggacc cgcccagcac cttgatgcac atcacagatt tggcacccgt
      301 gttgtcagca acatcgagtt tggattgcgt ttgaatcatt taaattagtc ccaacttgca
      361 ccagcgcctt ttccccaaag ggaagtcttg ccagtcagtc ttgggcccgt cgtccacacc
      421 acaaaccact tgcgatgctt ccgttaggca gaattctcag ctttttacag cgaagcccac
      481 gattattaac aaattcgctg cggctgtcaa cacctcttgc tgcaaattgt tcgccaggta
      541 cgacatccca tcagaatcaa ctacaaaaac agcctcatcc catttgcgga cgggcgcggg
      601 cagctattaa aaatgtagcg gccacaaccg gttcttcggc gctgggcgcg gggatggaag
      661 ggaatcaggc cggaaaagtc aggtactgat ccgccagggc cagaaaatcg gcgagccggg
      721 gatccacccc ggtttcctgc gccagcaact cagccacact ccgcgccagg gtcttggcaa
      781 gcgcggcgtc cagaaacagg agccgggatc ggcgcgccag cacatcctcc accgtacggg
      841 cgtactcgta gcgcgcggca aaacgcacca tcgcctccgt caggccaccg cccagcatcc
      901 tttccgcccc aggcagggcc tggacggcag ccgcatcgct cccatacaga tgcagcccgg
      961 gcgcatcgct aatcttgtga acggatggct gggcgcctac cagttggagg ctggccgtga
     1021 cgccgcccgg gcgttttgcc agcagttcag caccgaaaca gctgtccagc acgtcttcag
     1081 ccatggcgcg gtaggtcgtc catttgccgc cggtcacggt gaccagaccg cttttgctga
     1141 ccagtaccgt gtgttcacgc gacagcgcct gggtgctgtc gccatcttct tccggtggtt
     1201 tgaccagagg acgcaacccg acccagatgc tcttgatgtc ggccgggccg ggtgcccggc
     1261 tcaggtagcg cgccgactcg ccgaggataa agtcgacctc ttcgcgaaag gccaggggtt
     1321 cacgcgccag gtcgttgcgc ggcgtgtcgg tggtccccag gatggtcttg cccagccagg
     1381 gcacagcaaa cagcacccgc ccgtcggcgg ttttgggaac cagcagggcg tggtcggtag
     1441 gtaaaaacga gcgatcaacg acaatgtgaa caccctggct gggcgccacc atgggcttcg
     1501 cctcgcggcc tatggcctgg ccgtcgagca gtcgcagctg gtcgacccaa accccggtcg
     1561 cattcacaat acatttggct cgcagcgtaa gtgcccgccc actttcatga tcaactacat
     1621 gcaagcccac cagcttgtcc ccctcatgga cgagaccggt cgcagcacaa taattgacca
     1681 gcaaggcgcc ggcgcgcgcg gcactgcgcg ccagcgccag tgccagccgc gcatcgtcga
     1741 actggccgtc ccaatatttg accccgcccc tgagtccgga ctggcgggcc gttggctgga
     1801 gctgcaatgt ttcagcgcgg ttgaggaatt cggtggcgcc caagccggcc ttgccggcga
     1861 gcgcgtcata catcttgagt ccgatgccat aaaacggagc ctcccagaac ttgtaggaag
     1921 gcatcacaaa aggcaagggc tgcgccagat gcggcgcatt ggccagcagg cgcgtgcgtt
     1981 cgtgcagggc ttcgcgaacc agggaaatgt tgccctgcgc cagatagcga accccgccat
     2041 gaaccagctt ggtagcgcgc gaggaggtgc ccttggcaaa gtcgtgcgat tcgaccacca
     2101 cgacggaaaa tccgcgtgcc gcggcatcca gcgcgacacc gaggccggta gccccgccgc
     2161 cgatgatcgc aatgtcgtaa gtgtggggtg ccttgaggcg ttctatcagg tctgcgcgtc
     2221 gcgtcggcaa cggcggggaa tcaaaagtca tgggctccat tgtccatctc cgcagcaacc
     2281 ccggaacagg ttgcgccttc ctggctttct tgttggcaag cgcgcacgga cgcggtggtc
     2341 cgtgcgcggg cttgcatcag tcaagcaaca tgcgggccgg agcccgggcg gttgcggtca
     2401 acggactttg ccatccttcc aggcctgcag cagcttgtcg tagttgatgg tctcgccctt
     2461 gggcttttcg ttggccagct ttttccatgg cgcgccggtg tcgctgaggt acttggccgg
     2521 atcgcctttc gggttgagct tgggagcgca ccggtccatg cccgcacgct cgaggcgggc
     2581 catgacctgg tccatctctt ctgccagcgt gtccattgca ccctgcggtg ttttctcgcc
     2641 ggtcacagcc tgggccacgt ttttccacca gagctgagcc agcttcggat agtcgggcac
     2701 attggtaccc gtcggtgtcc aggccacacg ggccgggctg cggtagaact ccacgaggcc
     2761 accgagcttg ggcgccatgt cggtcatggc tttggagcgg atgtccgatt cgcggatcgg
     2821 cgtcaggcca acaatggtct tcttcagcga ggtggtcttc gcggtcacga actgcgcgta
     2881 cagccaggcg gccgccgtgc ggttttcgtc atgggccgag aagaaggtcc aggaacccac
     2941 gtcctggtag ccgttttgca tgccctgttt ccagtaaggt ccgttggggc cgggcgccat
     3001 gcgccacttg ggcgtgccgt cggcgttgac caccggcagg cccttcttgg tcatgtcggc
     3061 cgtgaaagcg gtgtaccaga agatctgctg ggcaatctgc ccctgcgccg gcaccgggcc
     3121 ggcctcgccg aaagtcatgc cggtggcttc cttaggagcg tattttttca tccagtccac
     3181 atacttggtc agtgcgtaga cggcggcagg cgagttggtc gcaccgccgc gggagaccga
     3241 tgcaccgacc ggtgtgcatt tgtctgccgc cacgcggatg ccccattcat cgaccggcat
     3301 gccgttgggg atgcccttgt cggccgtacc ggccatcgac agccatgcgt cggtgaagcg
     3361 ccagcccagc gatggatcct tcttgccata gtccatgtga ccgtagatcg gtttgccgtc
     3421 gatggtcttg acgtcttcgc tgaaaaaggc ggcgatgtcc tcgtaggcgc tccagttcag
     3481 cggcacgccc agctcgtagc cgtacttggc cttgaatttg tcctgcaggt ccttgcgcgc
     3541 aaacaggtcg gcacggaacc agtacagatt ggcaaactgc tggtcaggca gctggtacag
     3601 cttgccgtcc ggtgcagtgg tgaagctggt accgatgaag tctttcagat ccagccccgg
     3661 gttggtccat tccttgccct tgccggccat gtagtcggtc aggttcatga ccttgccgta
     3721 gcggtaatgg gtgccgatca ggtccgagtc cgaaatccag ccatcgtaaa tcgatttgcc
     3781 cgactgcatc gaggtctgca gcttctcgac cacgtcgcct tcctggatca ggtcgtgctt
     3841 gaccttgatg ccggtgattt cctcgaaggc tttggccagc gttttggact cgtattcgtg
     3901 ggtggtgatg gtttccgaca ccacggaaat ttccttcacc cccttggcct gcagcttttt
     3961 cgccgcttcg atgaaccatt tcatctcggc catctgctgg gccttgctca gcgttgatgg
     4021 ctggaattcg ctgtctatcc atttcttcgc ctcggcatcg ccggcgctgg cgttctgcat
     4081 gaccaccagg gccgctgcga gtgccagtgc tgtgtagcgc atcttcatga cttgtctcct
     4141 caattgatga cctcccccag tgtttccacg actcgcccgg ctccggggaa gcaatgagcc
     4201 gaatccgtac cctccgctaa cccttgcgca tgacaagcgc aagccatccc atggaaatga
     4261 caaaactgaa ccagatagac gggtcggcct gcaggcccag tgcggcaccc agctttccac
     4321 tgaggcccac aaagatcagg ttgatgtagg cggcgcccag caggccgatg aacagccggt
     4381 cgccgcgtgt tgtttccagc ggcaaaaagc cgcgacgcgc cgttgtcgga gacttgatct
     4441 cccagaccgt catgccaatc agcatcagga caatgcaggt aaaaaaaatg gctaccggca
     4501 gggtccacgc catccactca aacatgcctg tttctccttg tctgcggttt catacccgac
     4561 ccatcgcaaa gcccttggcg atgtagtgcc gtacgaacca gatcacgatc gcaccaggca
     4621 cgatggtcag cacgcctgca gcggcgagcg tcgcccagtc catgcccgaa gcagacaccg
     4681 tgcgcgtcat cgtggccacg ataggcttgg cgttgacgct ggtcagggtg cgggccagca
     4741 acagctcgac ccagctgaac atgaagcaga agaaagccgc cacacccaca ccggccttga
     4801 tcagaggcag gaagatgcga acgaaaaagc gcggaaacga ataaccgtcg atatacgctg
     4861 tctcgtcgat ctcgcgcggg atgccgctca tgaagccttc aagaatccag accgccagcg
     4921 gcacgttgaa cagcaggtgc gccagcgcca ccgcgatgtg ggtgtccatc aggcccaccg
     4981 tggtgtagag ctggaaaaag gggagcaaaa acacggccgg tggcgtcatg cggttggtca
     5041 gtagccagaa gaacacgtgt ttgtcgccga tgaagctgta gcgggagaac gcatacgccg
     5101 cgggcaaggc caccgtgatc gaaatcacgg tgttgatagc cacataaatc agggaattga
     5161 tgtagcccga ataccaggac gggtcggtga agatggtccg gtagttggcc caggtgaact
     5221 cctgagggaa aaaggaaaaa ctcgacagga tttcgccatt ggtcttgaaa ctcatgttga
     5281 tcatccagta gatgggcaac agcgcaaaaa caatgtaggc aatgaggaac agcgtcctcg
     5341 cctggaaacg gggcttattc accggtgttc tccttggtcg ccgtgccgac ccgttgcatc
     5401 cagttgtaga gaatgaaaca cagcagcagg atgatcagga aatagatcag cgagaaggca
     5461 gccgcgggcc ccagatcgaa ctggcccacc gccttctgcg tgaggtattg cgaaaggaag
     5521 gtggtcgcgt tgccgggccc gccgccggtc agtacaaagg gttctgtata gatcatgaaa
     5581 ctgtccatga aacgcaacag cacggcaatc atcaacacgc cgcgcatctt gggcagctgg
     5641 atgtagcgaa acaccgcgaa cttgctggcc ccgtcgatac gtgcggcctg gtagtaggcg
     5701 tccgggatgg aacgcagtcc ggcataacaa agcagcgcaa ccagcggtgt ccagtgccag
     5761 acgtccatga ccagcacagt gatccaggca tgcagcgcat tgccggtgta gctgtagtcg
     5821 aatcccattt cctgcagcat cctgccaagc aggccgatat cggcgcggcc gtagatctgc
     5881 cagatcgtgc cgaccacgtt ccacgggatc agcagcgaca gcgccaccag caccagaacg
     5941 gccgatgcct tccagccctg cgcgggcatg gacagcgcca acgcgatgcc caggggaatt
     6001 tcgacggcca gcacggcaaa cgaaaaagtc agctggcgcc agagcgccgc atgcaggtcc
     6061 tcgtcgcgca tgacggcggc aaaccattcg gtgccgacaa acacgcgccg ctcgggcgag
     6121 atgatgtcct gcaccgaata gttgactact gtcatcagag gcaggatggc cgagaaggcc
     6181 acacagatga tgaccggcaa caccagcagc catgccttct ggttgacggg ttttgtggtg
     6241 gtgctcatgg agccctcccg cttcctggcc tggaacggtt tcgcgctgcg ctcatgcgat
     6301 cagctcctca tttttgtaga aacaggtatg ggctccgagc agttgcagcc aggcggtgtc
     6361 gccggcttgc ggcgccgtcg cgccggaatg caggcgaacc ttcacagcac cctcacccac
     6421 cgtcgccgtc agcatcaaat gggtcccgat gtcctgcaca cgcacaacgc tcgcaggcac
     6481 ggcacccgca gcgccggacg ccgccagagc cacatattcg gggcgtacgc cgagtttgta
     6541 gtcgccggcc ggcagctccc gaccggccgg catgggcagc gtatgacccg cgatgtcgag
     6601 ggcttcgccg gtagcccttg cgggcaaaaa gttcattccg ggtgaaccga tgaaatgacc
     6661 cacaaacgtg tgctgcggcc gctcgaacag tgcatcggct gacccgatct gcacggcgcg
     6721 gccgcgggtc atcaccacca cttgctgggc gaaggtcaac gcctccacct gatcatgcgt
     6781 gacataaatc agtgtgagct tgagctcatg gtggatctgc ttgagcttgc ggcgcagttg
     6841 ccacttcagg tgcggatcga tgacggtcag cggctcgtcg aacagaaccg ccgagacatc
     6901 gggccgtacc aggccgcggc ccagcgaaat cttctgtttc gcatcggccg cgaggccggc
     6961 tgcgcgctgg ttcagctgac cactcatctc cagcatctcg gcgatctgcc cgacacgttt
     7021 tttgatctgg tcttccggca ttttccggtt gcgcagcggg aaggcgaggt tttcggccac
     7081 cgtcatcgtg tcgtagatga ccgggaactg gaacacctgc gcaatgttgc gctcctgcgg
     7141 cgtggcatgt gtcatgtccc gcccatcgaa tttcaccgtg ccctgcgacg gcgccagcag
     7201 gccggagatc acattgagca tggtggtctt gccgcaaccc gagggaccca gcagcgcgta
     7261 agcaccacca tcctcaaacg tcatctgcag gggcagcagc gcgtagtcgg catcttcgcg
     7321 cggattcgcc ttgtacgagt gcgcaagatc caggtcaatg cgggccatgt caatgccctc
     7381 ctttgcgctg cggcgcgacc agcagggcgc cgttcgcatc
//
LOCUS       DAIDFG010000152         7389 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_152_length_7389_cov_18.105536, whole genome shotgun sequence.
ACCESSION   DAIDFG010000152 DAIDFG010000000
VERSION     DAIDFG010000152.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7389)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7389)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7389
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152_length_7389_cov_18.105536"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1398 a         2337 c         2357 g         1297 t
ORIGIN      
        1 ccgaggtcat ggccttgcgc gggccatggt tcagcgaggt cgtcatgcac atcagcatgg
       61 tcgggcccgg cgtgaagatc agcagggcga cggccgcgag gtaaagaaga taggtggtga
      121 gggtcatgat ggggcgttga agcttcgctg cgaaacaagg caaaaaaatg ggaggcccga
      181 aacctcccat tttgaagcag aaaaaaatca gcccatcttg cagccggcac cagagtctgc
      241 aagcgccttg gcggccacgc cgatcacctt gttttccttg ttctcgacct ggcgcaggta
      301 gatgtcctgc accgggttgt gggccttgct catggtccac ttgccgcgcg ggctgtcgat
      361 cacggcggac tcgagcgcct tgtacagggc cggtttgctg ttcagatcgc ccttaaccgc
      421 attggcgccc tggaccagca gcaggccggt gtcgtaaccc tgcaccgcat acacatcggg
      481 ctggctgcgg aaggctgtgg cgtaggccag gcggaaggcc ttgttgcgcg gcgtgtccag
      541 cgagtcgctg tagtgcatgg tggtgatgat gccgtcggcc gcggggccgg ctgcgttcag
      601 aacaccttcg gtcaggaagc ctgagccgta caggggaatc tttcctttca ggccagccgc
      661 cgcgtagtcg cggatgaact tggcagcgcc gccgccggca aagaagcagg ccacggcgtc
      721 gggcttgagc gcggcaattt cggtcagcag cgcctggaac tcgacattgg ggaagggcag
      781 gcccagttcc ttgatgatgg tgccaccggc cgcggtgtag ctttccttga agccttcaaa
      841 ggcctcgtcg ccggcagcgt atttccaggt gatccagacg gcttttttgt ggccacgggc
      901 gaccatggcc tggcccagcg cgcgggtggg ctgcgaattg ctgaaagagg tgcggaatac
      961 attgggggcg cacaggccgc gggtggcggc atgcacaccg gcgttcggga tcaggctcag
     1021 cacgccggag tcgcgcgcca ctttctggat gcccatctgc acgcccgagt gcacggtgcc
     1081 gaccagcaca tcgaccttgt cgcgctgcac cagcttgctg gcgttttcaa cgcccttgga
     1141 aggctcggac tcgtcgtcca ccttgaagta ctcgatctcg cggccgccga gcttgccgcc
     1201 ttgctggtcg atggccaggc ggaaaccgtt ttcaatcgcc acgccgagct gcgcgaaggt
     1261 gccggtgtaa ggcagcatga aacccacgcg caccttgccg gactgcgccc gcaccacttg
     1321 agggagcagc aggccggtgg aggcggcgcc gatgacggcc gcactctggc tcaggatcaa
     1381 acgacgtgaa ggaaggcgtg aggtcatgga taactcctgg ttgggacgga acgaatagcg
     1441 ccggaaggct ttgatggtaa cggcgcggtg ctgcgaaaac cctagtgaaa aacccgagat
     1501 ttaggattaa attgtttacg tttaaagcaa ataccctgcc agactgcggg ttcaggccgc
     1561 gacctgctgg cgcaacttga agcgctggat cttgccggtc gcggttttcg gcagctcgtc
     1621 cacaaactct atccagcgcg ggtacttgta aggtgccagt tgctgcttga cgtgcgcctt
     1681 gaggtcgtcg gccgaggctg cctgaccttt cttgagcacc acaaacgcct tcggcttgac
     1741 cagctggtcg gcatcggcca agccgaccac cgccacttcc agcaccgccg ggtgggtcat
     1801 cagcgacgcc tccacttcga aaggcgagac atagatgccg ccgaccttga gcatgtcgtc
     1861 ggtgcggccg ctgtaggtgt agtacccgtc ggcatcgacc gtgtatttgt cgccgctgcg
     1921 tgtccactcg ccggcaaacg tggctttggt cttggcgcgg ctgttccagt acatcagcgc
     1981 cgaactcggg cccttgatct gcagctcgcc gatctcgccg acgccgcagt catgtccctc
     2041 atcaccgacg atgcgcagct cgtaaccggg cacggccttg ccggtgctgc cgtaacgcac
     2101 ttcgccaggc cggttgctca ggaagatatg cagcatctcg gtcgagccga tgccgtcgag
     2161 gatgtcgcag ccatactcgg cggcccactt gttgccgatg ccggcgggca gggcctcgcc
     2221 ggcgctggtg ctgacgcgca gattcaacgc agcccggggc gggcgggccg ggtccgccag
     2281 cagggaggcg tacagcgtgg gcacgccgta gaagatggtg ggctggtgct ctttcaggat
     2341 gcggaagacc tcggccgtgg tcgggcgtgc cggcagcagg atggccgtgg cgcccacgct
     2401 catcgggaag gtcaggccgt tgcccagccc gtaggcaaaa aacagcttgg ccgcggaata
     2461 caccacgtcc gtctcctgga tgcccagcac gccgcggccg tacagctcgg cggtctggat
     2521 caggtggctg tgcaggtgca ccgtgccttt gggcgcgccg gtggagccac tggagtacag
     2581 ccagaaacag gcgtcgtcgg cacaggtcgg cgctacgatt tttgtagctg gttgtgcccg
     2641 gatcatctgg gccagactcg catgcggcgt cgcatcgtcg acgccggcga cgatcacgct
     2701 gcgcagcgtg ggaatgttcc cgatcagcgg ctcaaacgcg ggcagcagcg gctgggacac
     2761 gatcaggccc tgggcgcgcg aatcgcgcag catgaagtcg aagtcgggcg tggtcagcag
     2821 ggtgttgacg gccaccggta ccacaccggc caggatgcag cccagaaaag ccaccggcca
     2881 ttcgggggtg tccagcatgg ccagcagcac acggctctcg ggcgcgaagc cttgggctcg
     2941 cagcgcattg gaaaaacggt gcgcctgttc gtccagctcg ccgtagctca gcgtcgcacc
     3001 gctgactgcg tcgatgaagg cggtcttgct ggggcggctg gcattgcgct ccagcaggtc
     3061 atgggcggcg ttgtagtcgc ggggaatgtc aacgcgaggg gggcttaccg aatggtcggc
     3121 gcggctcagg ttcatggggt ctccggatgc tctcgttttt atagctgcct gcgcccgcca
     3181 ctattgggct ggaggctttt ttcagtaaga ctcaggcggt ttcggtgctg ggcgtgctgc
     3241 tcagtgccag tgccagccac tcgcggcacc aagcggcacc ctcggtctcg ggcagggtgc
     3301 cggcgctggc gtcgtggcac cagacctccc cgatacgctg cgcgcccagg ccctgcaggc
     3361 gctcgtcaaa cttcttgccg ccgaaacaga aggtctgcag gtaggtgcgg tcgccgaggg
     3421 cgatcacgcc gtagcgcacg tggcccagga atttgggctg ggcatcgaga gagtcataca
     3481 gcagccgggc gttgtccggc acatcgccct ggccgtaggt ggatgaacag atgagatata
     3541 ttgcatcttc ttcggcggcg ctgtcgaaga cgctgatgtc caggccgtcc atcagctgaa
     3601 cctcgatatc actgacgaga tcggcgcaat ccatctggat ggcttgcgcc acgtagtcgg
     3661 ctgtgctggt catggtgccc acgagaattt tgagtttcat cagtgatctc cgaaaaagat
     3721 gcaataaact gcttgacaag cgaattacgt gaagtatagt gcaatattga tttaacgcaa
     3781 acttttttca aataacgcag atgtccaacg tcgaaaccct gtccgaccag gcgaacaagg
     3841 ccacgctcgc cgagctgggt gcccgggtgc gcgcctggcg cgcccgccgc ggcatgacgc
     3901 gcaaggcgct ggcgagtgac tccggcctga gcgagcgttt cctggccgac gtggaaagcg
     3961 gcaagggcaa tgtctcgatc aactcgctcg aagccgcggc ccgcgcgctg aacatctcga
     4021 ttctggaact gctgcaggac gcaccgcgcc cggcgctggc ccgtgtccag gggctgctgg
     4081 gccggctcga cgacacgcag ctggaccagg cctacgcact gctcggcagc accttcggcc
     4141 tgagcgatgc gctgggccgc gaacagcgca ttgccctgat cggcctgcgc ggtgctggca
     4201 agtccacgct gggcctgaag ctggccaccg agcgcggcgt gccttttgtc gaactcgacc
     4261 gcgagatcga gcgcgaagcc ggcaccagca tgaacgaaat cctgctgctg cacgggcagg
     4321 ccggctaccg ccgctacgag cgccgcgcgc tgtttcgtat tgccgaggac catgccgacg
     4381 gcgttgtcat gaccaccggc ggcagcatcg tcagcgagcg cgaaaccttt gacctgctgc
     4441 aaagccgctt ttattgcgtc tggctcaagg ccagcccgga agaacacatg gcacgcgtgg
     4501 tggcgcaggg cgacatgcgg ccttttactg ccacccgcgg cgccacggac gaggcgatgg
     4561 aagacctgcg ccgtatcctg gccagccgcg aagcgttgta tgcccgcgcc gatgccgtgg
     4621 tggacaccgc cgcccgaacc ctcaaacaaa gcctgaaaga cctggaacgc gccgttcctg
     4681 cccaggcgca acccaaggag actccatgaa cgccatgacc gaaccacttt tgttcaaaca
     4741 ggtgattgag ggccaggagc gcgagctcgt gagctttgcc acacacccga ccaagtacca
     4801 gcactggacc ctgagcgtgg agggcgacgt tgcgcgcctc aagctcgaca tcaacgaaga
     4861 cggcggcatc aagcccggct acaagctcaa gctcaacagc tacgacctcg gcgtggacat
     4921 cgaactgcac gacgcgatca accgcatccg tttcgagcat cctgccgtca aggtggtggt
     4981 gttcgagtcg ggcaaggaaa agatctggtg ctcgggcgcc aacatctaca tgctgggcct
     5041 gtccacgcac ggctggaagg tcaacttctg caaattcacc aacgagaccc gcaacggcct
     5101 ggaagactcc tcgcgccatg atggcctcaa ggcgatcgcc gccgtgaccg gcgcctgcgc
     5161 cggcggcggc tacgagctgg ccctggcctg cgaccgcatt gtgatggtcg acgaccgcag
     5221 ttccaccgtg agcctgcccg aagtgccgct gctcggcgtg ttgcccggca ccggcggcct
     5281 gacgcgcgtg accgacaagc gcaaggtgcg ccgcgacttg gccgatatct tctgcaccac
     5341 gtccgaaggc gtgcgcgccg accgggcccg cgactggaaa ctcatcgacg cctatgccaa
     5401 gccgcagcag ttcagcgcct tgcttgctac tgaaatagaa gcgcttcgcg cccagtccac
     5461 aagggctgca gcctcaaaag gcattgaact cacgccgctg aaggtgctca tcgacgatgc
     5521 cggttaccac tacagcgtgg tcgatgcgca ggtggaccgc gaaaaacgca ttgccaccat
     5581 caccgtcaag gcgccggccg ccgcgattga gtccacgccg gcagccatcg aagccgccgg
     5641 tgccaactgg tacccgctca agctgcagcg cgagctcgac gacgccatcc tgaatctgcg
     5701 caccaacgaa ctcgaagtcg gcacctgggt catcaagacg catggcgacg ccggccaggt
     5761 gatccgcatg gacgaggtgc tggcccagct caaggaccac tggctggtca aggaaaccac
     5821 cggcgcgctg cgccgcacgc tggcgcgcct ggacgtgacc agccgctcgc tgatcgcgtt
     5881 ggtcgatgcc ggttcctgtt ttgccggcac gttttacgaa ctggccctta gctgcgaccg
     5941 catctacatg ctggacctgc cggattcgcc cgacgtcgcg cccacgctgc aactgagccc
     6001 cgccaatttc ggctggttcc cggccgtcaa tcacctgaca cggctgcaga cccggtttta
     6061 tgaagacgag gccaccatcg cgcagctgca aaaactcggt gacagcccgc tgcgtgccga
     6121 tcaggcgctc gagctgggcc tggtgaccct gagccccgac gacatcgact gggatgacga
     6181 gatccgcatc atgctggaag aacgcgccag cctgtcgccc gatgcgatga ccggcatgga
     6241 agcctcgctg cgctttcccg gcaaggagac catggaaaca cgcgtgttcg gccgcctgtc
     6301 ggcctggcag aactggattt tcatccgacc gaatgcggtc ggggaagacg gtgccctgaa
     6361 acttttcggt accggcaaaa aagcgaaatt tgattggaaa aggatttaac accatgagca
     6421 ccattgacct gcaagccctg atccccaaca acgtcaacct cggcgagaac cgccagctgc
     6481 agcgtgcgct cgagcactgg cagccggcct tcatgagctg gtgggacgaa atgggcccga
     6541 gcgacttcaa tgccaaggag gtctacctgc gcaccgccgt cggcgtggat gcgtccggct
     6601 gggccagcta cggctacacc cccatgcccg actaccgctg gggcattttt ctggccgacc
     6661 aggaagaaaa ccgcaagatc ggttttggcg accacatggg ccaggacgtc tggcaggaag
     6721 tgccggggga ataccgaagc gccttccgcc gcctgatcgt cacccagggc gacaccgagc
     6781 cggcctcggt cgagcagcaa cgcctgctgg gccacaccgc gccttctttg tacgacctgc
     6841 gcaacctgtt ccaggtcaac gtcgaggaag gccgccatct gtgggccatg gtctacctgc
     6901 tgcacgcgca tttcggccgc gacggccgcg aggaagccga agagctgctg acgcgccaca
     6961 gcggcgacgc cgacaagccg cgcatcctgg gcaccttcaa cgagcccatg gacaactggc
     7021 tgagcttctt catgttcacc tacttcaccg accgtgatgg caagttccag ctcaagtcct
     7081 ttgccgaaag cgcgttcgat ccactggcgc gcaccacgcg gttcatgctg acggaagagg
     7141 cccaccacat gtttgtcggc gagaccggca ttggccgcgt catcaaacgc acgctggaag
     7201 tcatgaaaga gctggacacc gacaacgtgg acacgctgcg caaggccggc gtgatcgact
     7261 tgcccaccgt ccagaaattc atgaatttct ggttcacctc ctcgctggac ctgtttggct
     7321 ccgaggcctc gtcgaacgcg gccaactact tcagcaacgg catcaagggc cgaccggacg
     7381 aagccaagt
//
LOCUS       DAIDFG010000153         7373 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_153_length_7373_cov_16.462831, whole genome shotgun sequence.
ACCESSION   DAIDFG010000153 DAIDFG010000000
VERSION     DAIDFG010000153.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7373)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7373)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7373
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153_length_7373_cov_16.462831"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1418 a         2413 c         2290 g         1252 t
ORIGIN      
        1 gctgggcgca aaaatcgcgc tgaaaatgat caggaggtac aactcgggca tggagcccca
       61 gatttcgatg aaacgctgga aggccaggtc ggtcttgccg cccacatacc cctgcaccgc
      121 cccggtgatg atgccgacca ctacaccgat cagcgtcagc gccagcccga acagcacgct
      181 gaggcggaaa ccgtagatca gctgggccag caggtcgcgc ccgcggtcgt cggtgccgaa
      241 gatgttgtcg cgcgacggcg ccgacggatt gggctccttc gcaaaatagt tgatggtgcg
      301 cgggccgtag ggattgagcg gataaaccga ccagttgtcc ccttccctga gtttggcgcg
      361 gacaaagggg tcgaggtagt ccgtcggtgt atcgaagtcc ccgccaaagg tcttttccga
      421 atagtcccgg accagcggaa agtagaacgc cccctcgtac cgcaccagca gcggcctgtc
      481 gttggacaac acctcggcaa agaggctcag taccaccagc acgcagaaaa tcaccagact
      541 gcggtagccc agcttgttgc gccggaagcg ctgccaggcg cgtctggacg gcgatacgga
      601 aaggggcgag gcagggatcg tcatgaatca atcaaacttc acgcggggat caacccagac
      661 ataacaaagg tccgagacca gcttggtcag caggccgatc agcgtgaaca ggtacagcgt
      721 gcccagcacc accgggtagt cgcgccggat cacgctttca taactgagca ggcccaggcc
      781 atcgagcgaa aacagggttt cgatcagcag cgaaccggtg aaaaaggcgc cgatgaaggc
      841 cgacggaaac cccgtgatga tggggatcag cgcattgcga aacacgtgtt tccagagcac
      901 ctggcgctcg gacaggcctt tggcccgcgc cgtcagcacg tactgcttgc ggatttcctc
      961 gagaaatgag tttttggtca ataccgccgt gaccgcaaaa ctgcccagca ccatggccgt
     1021 gaccggcaag gtgatgtgcc acaggtagtc ggtgatgcgc gcgccccagc tcagggtctc
     1081 ccagttcgac gaggtcagcc cgcgcaaggg aaaccactgc agctgcccgc caaaaatcac
     1141 gagcaacgcc acgcccagca caaagccggg aatggcgtaa ccgatcagca cgatcagcgt
     1201 ggtgacgaag tcaaaacgcg aacccgcgcg caccgccttg gccacaccga gcggcactgc
     1261 gatcaggtag ctgatgaaaa aagtccacag cccgaggctg atggacaccg gcagcttttc
     1321 cttgaccagt tgccagacgt ccttgtgctg gtaaaaactt ttcccgaggt caaaccgcgc
     1381 gaactgcccg agcatctgga aaaaccgctc gtgcacgggt ttgtcaaagc cgtacagcgc
     1441 cctgatttcc tcgatgcgct gcgcgtccag gccctgacgc ccgcggtaac cggacccggt
     1501 ggccgccgcc cgctcaccgc cagaatcacg cccctgcaac tgcgccacca tctgctcgac
     1561 cgggccgccg ggcacgaact gcaccatggc aaaggtcagc agcagcaccc ccagcagggt
     1621 gggaatcatc aacagcaggc gtttgaggat gtagctggcc atggttatgc agcggacagc
     1681 gaatgcatga agtgaagagc gtggagggtc atttgttcgt gctcgaagcc caccaggtgc
     1741 tcatggccca ggtgtcggcc tggtaatacg gcggaatcgt cgccggcaac acaaacggcg
     1801 caggccggta accgatcagg aaggcgtcgc cgtaatactg cggcaccgag taaaaaccgt
     1861 gcgtcagcac ccggtccagc gcacgcatgg cggcggccag ctcgggcctg gttttggctg
     1921 tcactacctt ttgcagcaag gcatcaaccg ccggatcggc aatgccccag acattggacg
     1981 agcctggcgt ttttgccgcc tcggatccga aacgctccag caactcgcta cccggtgcgg
     2041 tcgagcccgg caggcgcaac gtcgtgacct cgaagtcgaa cgcgtccatc ttctgcttgg
     2101 acagggaaaa atccacaacc cggtaagtca tggtgatgcc gagcttttcc agcgctttct
     2161 ggaagggccc gacgatgcgc accagcgagg gctggtcgtt gaggaactcc atcgtgaagg
     2221 cctcgccttt ggcattgcgc aacgcgccat cgcggtaggt ccagccggct tcgcgcagca
     2281 gcgcctgggc ctgacgcagg ttctgccgca ggctgcccgg cggcgtggtg gcgggagcca
     2341 ccggcaccgc gccaaacacc gcagggctga gcctggcgcg cagcggctcc agcaacgcca
     2401 gctcgtcggg cttgggcagc ccctcggcat gaaattcact gttcgggaaa tagccgttga
     2461 cgcgtttgta gatgccgtag aacagctggc ggttcatcca ctcgaagtcc atggccagcc
     2521 cgatggcgcg ccgcacgcgc acgtcctgga atttttcctt gcgcaggttg aacacatacc
     2581 cctggaagtc gccggggttg cggttctcga aagcccgctt gacgagttcg cccgaggtga
     2641 actgcttgcc ggtgtactgg cgggcccagt tgcgcgagat gaactcgcgc atgaagtcgt
     2701 attcaccggc tttcagccct tcaaaacgcg aggtttcgtc gaggtagatc ttgaaggtga
     2761 tgcgatcgaa gttgaactga cccttgcggc tgggcagctc ggcgccccag taggccgggt
     2821 cgcggacata ggtgatgtcg cgccccatgg ccaggctggc gggcttgtag gggccggagc
     2881 ctatcggcag gtcggacacg atgcggtcaa acggtttgcc gccgccccag gcgcggctga
     2941 acaccggcat gccgcccacg accagcggca actcggggtt gggcactgcg aaatcgaaac
     3001 gcaccacccg ttcggagagc acctgcgcac ccttgacctc ggcataaatg ctgcggtatt
     3061 gcggcgcggc cagcttgccg gtcaactgcg tgaacgaatg cagaacatca gcggccagca
     3121 ccggcgagcc gtcctgaaag cgcgccttgg gatgcaggcg gaaggtcgcc gacagccggt
     3181 ccggcgccac cgcgacgtcg tcggccagca acccgtaggc ggtggtcggc tcctcggcat
     3241 tgccggtcag caggctctct atcatcagca tgccgatgcc gtaaggcgcc gtgccgcgca
     3301 gcgtgaaggg gttgaacttg tcgaagttgg tgggccgggt cggcggcacc atgcggatct
     3361 cgccgccctt gggcgcggcg gggttgacgt aatcgaagtg ggtgaagccc ggcgggtact
     3421 tgatgtcgcc gaactgcgca taggcgtggg cagcccagga cgccggagcc aggcagacca
     3481 gaacggccat cgtcaaggcc cgcaaaaacg acaaatcccg catgcgacaa ttctgcacta
     3541 cttaagttgg caaggagcgc atgcacgcag cgcgcccttt gcccatttcc tcgcacacac
     3601 tggagcacaa gcatgggcac acccacggga tttctcaccg gcaaaaaact gttgatcacg
     3661 ggcgtcctgt ccaaccggtc catcgcttac ggcattgcca aggcctgcca tgctcagggc
     3721 gccgaactgg ccttcagcta cgtgggtgaa cgtttcaagg accgcatcac cgaatttgcc
     3781 gccgacttcg actccacgct gatttttgac tgcgatgtcg gcgacgatgc gcagattgcc
     3841 gccctgtttg ccgacctgac caagacctgg ccgacctttg acggctttgt gcacagcata
     3901 ggctacgcac cacgcgaggc gatcgcgggg gatttcctcg aaggcctgac acgcgagggt
     3961 ttcaagattg cccacgacat cagcgcctac agttttccgg ccatggccaa ggccgccctg
     4021 ccccacctca accccaagtc ggccctgctg accctgacct atctgggcgc ccagcgcacc
     4081 atccccaact acaacaccat gggcctggcc aaggccagcc tggaagccag cgtgcgttac
     4141 ctggccgaat cgctgggccc caaaggcatg cgcgtcaacg gcatcagcgc cggcccgatc
     4201 aagaccctgg cggccagcgg catcaaggac ttcggcaagc tgctgggcat ggtggccgcc
     4261 gcctcgccgc tgcggcgcaa cgtcaccatc gaggatgtgg gcaacgtggc cgcctttctg
     4321 ctgtctgacc tggcttccgg cgtcaccgcc gaaatcacct atgtggatgg cggtttcagc
     4381 cacacggccg gcatcggccg cgagaccgag gcgagctgaa ccggctcggg gttccatccc
     4441 gggtttggag tcaaatatgg cctgagcccc cgatccacgg gcgccagcag ctatataaaa
     4501 gtgagcatca tggggcccgc gccggcgggt caggcccggc gccagtactc atacgtccac
     4561 gctgttccaa agcccgcgcg ccggtacagc gcctgcgcgg cggtgttgcc ggcttcaacc
     4621 tgcaaaaaca cccgcgccat gccccgcgcc agcgcagcgt ccgccagtcc tgccagcact
     4681 cgtccggcga gaccctggcc gcggtgacct gcggcggtgc gcatgccgtg cacgctgacc
     4741 cagccatgac caaaagccgc caccccgccc gcgagcacct ggccgtcttc acgaacgctg
     4801 gcatacaccg agccctgcgc ccggcccaat gccctgaccc ggcaggcgcc gtccaccgga
     4861 tcaaaaccct cgcccaggaa aagcgccgtc caggccgcat ccggcgcagc atggacctcg
     4921 gcgctcgcct gcgatgacac ctcgcgcatg cgcgccaccg tgcccagctg caccagcgtg
     4981 ggtttgtcgg cgccgtaacc gcgctggcgc agttcatggc gcaagccgtc aaaacaggcg
     5041 tcgatggcca ggcgaaacac cgccggaaga ccccgcgccg cgtaatacgc ctcgatgcgg
     5101 cccagcacgc ccgggtcggt gtccatatgc tgcagcggca cggcacttct ggcacggcct
     5161 atggttccgt gatcaacagg caacagccag ccctcccgct cctccacggt ttcgggggcc
     5221 acggcggcca gggtggcctg ttctatggaa gcaacatctt tggcgttcat gaatgaagtg
     5281 tgtaccggtt cagacttgca gcaagcccgt gctcgaccgc acaatcgtca ggcgcaggcc
     5341 aaaaaagcgg gcaaaacagg gaaaacggga aaaggccaat actactattt tgatagctga
     5401 ctccgccctt tcctcgggcg cttgcggcat gaatcatgca ataaatccac cgacatgacg
     5461 gcagctccac cacccaccct cactctgact ctcacgatca cccgcgcgag cggcgctttc
     5521 cttcgcatct gcgcgctgcc ctggctgctg gcgttgacgg ccctgctggc gcctgccctt
     5581 cccgccgcgg cggccggcaa cagcccgcca ctgatgctgg ccaatgtcta ccaccccggc
     5641 atcgtgctgc aggagtactg ggtcagtgaa aaactcgacg gcgtgcgggg ctactgggac
     5701 ggcgaaaagc tgctgacccg cggcggcgaa cgcgtcgccg ccccggcctg gttcaccgcg
     5761 ggctggccca ggcagcccct ggacggcgag ttgtgggccg gccggggtca attctccaaa
     5821 gcggtgtcca ccgtgcgcca gcaaacaccg gacgacgccg cgtggcgcgc catgcgtttc
     5881 atggtgttcg atctgcccac gcaggcaggg accttcaccg agcggattcc ggccctccat
     5941 ggggtggtca gccgcatcga ccagccctgg gtgcaggcgg ttgcgcagtc ccgggtggcc
     6001 agccatgccg aactccagcg cctgctggcc aaaaccgtca ggctgggcgg cgaagggctg
     6061 atgctgcacc gcggcgcctc gctctaccag ggcctgcgca atgacgacct gctcaaggtc
     6121 aagacgcacg aggacgcgga agcccgcgtg ctggcccagt tgccgggcca gggcaaacat
     6181 gccgcggccc tgggcgcctt gctggtcgag acggtgggcc cggacggcct gccgggcaag
     6241 cgcttcaagc tgggcaccgg tttcagtgat gaacagcggc gccacccgcc gcctgtgggc
     6301 aacatcgtca cctaccgctt tcgcggcctg aatgacagcg gtattccgcg ctttgcgagt
     6361 ttcctgcggg ttcgggagga catggcgcca gcacggccct gagcgccgct gcgccggtcg
     6421 gcaggccggt cgcgccctga ttactctttc acgcagtcgg cgtaatagtg cttcttgccg
     6481 tcggcatctt cgatttcgac cagaccgtga atgtcggtct cgaaacccgg gcactgcgcg
     6541 ttgaactcgc gcgagaactt cagatagtcg acgatcttct tgttgaatac ttcgccggga
     6601 atcaagagcg gaatgccggg cggataaggc gtgaccaacc ccaccgtgat gcggccctcg
     6661 agcttgtcga tctcgacgcg ttcggtcttg tgcagcgcga tgtgcgcgta ggcgtcgctg
     6721 ggtttcatgg ccggagtgag gtcgctcagg tacatgtcgg tggtcaggcg cgcaatgtcg
     6781 tacttggcgt agagcgcgtg gatgtgctgg cacaggtcgg ccagacccat tttttcgtaa
     6841 cgcgggtact tctgacagaa ctccgggagg atgcgccaca tcggctggtt cttggcgtag
     6901 tcgtccttga actgctgcag cgcggtcagc atgctgttcc agcggccctt ggtgatgccg
     6961 atggtgaaca tgatgaaaaa gctgtagaga ccggttttct cgaccaccac gccgtgttcg
     7021 gacaggaact tggtcacgat gctggccgga atgccggtct tggcgaactt gccgttcagg
     7081 tccatgccgg gtgtgaccac ggtggacttg atcggatcca gcatgttgaa gccggtcgcc
     7141 atcttgccga aaccgtgcca gttgacaccg gccttcgcgt tcttggactc gcccttgatg
     7201 atccagtcat ccgagcggcc aatgccctcg tccaccagct tctcggggcc ccagaccttg
     7261 aaccaccagt cgtcgccgta ttcctcatcg accttgcgca tcgcgcggcg gaaatccagc
     7321 gcctcggcaa tgctttcctc gaccagcgcg gtgccgccgg gcggctccat cat
//
LOCUS       DAIDFG010000154         7356 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_154_length_7356_cov_17.284482, whole genome shotgun sequence.
ACCESSION   DAIDFG010000154 DAIDFG010000000
VERSION     DAIDFG010000154.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7356)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7356)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7356
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154_length_7356_cov_17.284482"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1348 a         2174 c         2407 g         1427 t
ORIGIN      
        1 ggccgcggaa atccggggtg aggatttcga tgcgggtggc cggcgacttc tcgcgcaccg
       61 ccgcgatgca cagcgcgaaa tgatcggcgc cgccgtcgcg caggtcgtcg cggtcgacgc
      121 tggtgatcac cacgtacttg agcttgagcg cggcaatcgt cttggccagg ttctcgggct
      181 cgttgacgtc cagcgggtca ggccggccgt ggccgacgtc gcagaagggg caacggcgcg
      241 tgcacttgtc gcccatgatc atgaaggtcg ccgtgccctt gccgaaacat tcaccgatgt
      301 tggggcagga cgcttcctcg cataccgtca ccagcttgtt gctgcgcagg atgtccttga
      361 tttcgtaaaa gcgcgtgctc gggctgccgg ccttgacgcg aatccactcg ggcttcttga
      421 gcagctcgcc ctgcaccacc ttgaccggga tgcgcgacag cttggccgcg gccttctgct
      481 tggccagcgg gttgtagtct tcctggctct ggatatcgcg aacaacagga ttgacagctt
      541 ctggggtgct catggtgtgc ttctttggaa ggaatgtggt cagggcgcga ggtaggtgac
      601 gagcttgtgg cccagcacgt ccgcggcctc ctgccagctg gccgataccc cgattgtaga
      661 aaggtctgtg gtttgcagcc cggcataccc acaggggttg atgcgcgaga agggttcgag
      721 gtccatcgcc acattgagcg cgacgccatg gtaggtgcag tgccggctga ccttgatgcc
      781 cagcgcggcg atcttgccca ggccgcggaa agggtcggcc gggttgagcg ggccgctcag
      841 cgccgcgtgc gaggccgggt ccgcgagacg gacataaatg ccgggcgagc ccgtcacgcg
      901 gtgcccggtc acgccgaaat gcctcagcgt cttgatgact gcttcttcga tgcggtagac
      961 atattccttg acgaaatagc cggcgcgttt caggtcgatc agcgggtagg ccaccacctg
     1021 gcccgggccg tgatacgtga cctggccacc gcggtcggtc tgcaccaccg gaatgtcgcc
     1081 gggggccagc acatggtcag gcttgccggc caatccttgt gtatagaccg gtggatgctc
     1141 acaaatccat agctggtcgc gcacgtcctg attgggctgg aggcctgttt ggcttgtaac
     1201 ttcacggcgg ctggcggtga aggcctgcat ggctgcgtag gtgggcgggt agtccacccg
     1261 gccgagctgg cgcacatcaa aggacatggc aggcttttgc atgcgggtgc cttacagcac
     1321 cactttgacc atggggtggg ttgacagcgt gcggtacagc tcgtcgagct gctccctgga
     1381 ggtggcggtg acggtgatgg tcacgcccag gtatttgccg cccttgcttt cgcgcagctc
     1441 gatggtggtc gcgtcaaacg ccgggtcgaa ctgatgggcg accatggtga tggcgtgcac
     1501 cagcccgtcg accttttcgc ccatgacctt gatcggaaac agcgaggggt attcgatcag
     1561 cgactcctgg cgcggcgttt cgcctggcgc gggcggtttg ggtggggtgt tcatgccttc
     1621 tccgtctgcg cctgtttggc gcgctggtag gccgcgtgca gcttcgcgta aaccgggccg
     1681 ggctgaccgt tgccgacggg cttgccgtcg agcgtggtga cggccagcac ctctttggtg
     1741 gccgacgaca gcagtagctc gtcggcggcg aaaacttcct cgcggctgac ccggcgcagt
     1801 tcaaacggga tactggcatc gcggcagatc tcctcgatca ggccgtaacg aatgccttcg
     1861 agcaccaggt tgtccttggg cgggcccagt accttgccgc cctgcacgac ccagacgttg
     1921 caggcggccg cttcgctgag gaagccgtcg cggaacatca cggtttccac cgcgcccacg
     1981 tcggcgctga tctggcgcgc aaacaccgcg cccagcaggc tggtgctctt gatgtgggcc
     2041 ttgctccagc ggaagtcgtc ggcggtgacg caggccacgc cgtgcgcgcg ctgctcgggc
     2101 gtcgggggct tcatggtgtt cgtcatgaca aacacggtgg gtgtcaggtc gggcggcatc
     2161 acatggtcgc gcagcgccac gccgcgggtg atctggatgt agaccagctg gtcggttttt
     2221 tccgccgagg tgtgcatctg cagcgcgtag tcggcgacca acttgagcgt gatctcgcgc
     2281 cactggacgt gggacagcgg attggcgatg cgcaactccg ccagactgcg gtccagccgt
     2341 gccatgtgct gggcaaagcg gaagggctga ccggcataca ccggcaccac ctcgtagacg
     2401 ccgtcaccga agatgaagcc gcggtccatc acgctgacct tggcgtcccg cagcgcggtg
     2461 tattcgccgt tcaggtaaca cggtgtggcg ggcaggggcg tgatcgaagc ggggctggaa
     2521 gtgctcatca tgggattcaa aaaaaggtgg ggagaggatt gtcgcgcagg ctgtgtttga
     2581 cgctagcggc catgacgctg gacacgccgc ttgaaccaga cgcgaaagcg ccagatgagg
     2641 ttgaccagca catagccgcc ggccgagccg gcgacggcaa aaatggccat gccgagcacg
     2701 gccggccagc caaaacgctc cagccagccc atgacatcga agccggcgct ggctgacgcc
     2761 gcctccagcg ctgcgctgcc ggcggccacc tcgtggggtg cgatgcccag gatcaggctg
     2821 cccagcttgt aagccagcca gagccagaag ccgacggtga acgggttcgt gatcagcgtc
     2881 acgccggcgg ccaccgggat gttgccgcgc caccagatgc agtggacggc ggccaccagg
     2941 atctgggcga caggtaatgc aaacgcccag aacaggccga tggcgacgcc gcgggccacg
     3001 gcctcgtgct gcacatgcca gagcccgggt tcgcgcaggt gatgcgccac cggcttgagc
     3061 cacgggtggg ctgccagcgt ttcgcggctg attcccttgg tgaagcggtc cgtccaggag
     3121 ggtgatggcg tgctgcgttt tctgcgtgtg ctcatggccg cgccccgacc ggaggacagc
     3181 gtgtttcgtc catcgacatc acgctggcgg cccgcaccct caggcccaga aaatcacggc
     3241 cgcaatggcc agggtgccga gcaccaggtt gctcatggcc agggtggcga tctggccgac
     3301 acggcgtccg ccctcgggcc agtccgcggc agccacggcc agtttcaggc gccggtaggg
     3361 gccaaaataa agatgaccga acagcgcaaa catcagcagg ccgatgccga acatcaggtg
     3421 ccagcccagc ggcgcgtttt tcatgccgac ggccagcagc atggccaggc cggtaaacag
     3481 cagcagcgct atggcgggcc agaccagcac aaaaaacttc tgcaagacga gtgcgatcag
     3541 cggcaggcgc tgcgacgggg gcagttgcgc ggcggcggca ggccgcagcg caaaaagcat
     3601 gaagctgatg ccgcccagcc acacgatggc ggcggcgata tggaggaatt tcatcagggc
     3661 aggcatgcaa gggtctttca aaaatgacgc gctgcaagag cggcaatgac ccgccagtgg
     3721 cggggcctgc agcggccggg aagtggattg tcgcttgcaa aacgtccccc tgtggaccgc
     3781 gcggccatgc ctcccgccaa agtttggtgc ggggactttc aggaacgggc gggtttctac
     3841 gtataatcaa gggctttgct aataaccggc ctgtaacctg ctcgtaattt gtgatggtca
     3901 aaatgacaga ttcggtggtt cttccgttgg caaaggctcc gactcaggta actgaggcgg
     3961 gggaagaaaa tttcaagccg ctgacgcgcg aagaagcgca gaagctgcgc gaagccagtc
     4021 cttcgctgtc gccctggtgg gtggtggccg ggcaactggt ggccggagtc ctggtggcct
     4081 gtgttgcctg ggctttgacc ggaagacaaa acgtggcctg gtcggcgttc tacggcgcgc
     4141 tggcggtggt gatccctgcg gcgctttttg tgcgcgggct gaccagcaag gtgtcttcca
     4201 tgaatgcggg cgctgcggtg ctcgggttct ttttgtggga aatggtcaag ataggcctga
     4261 cgatcgcgat gttgttcgcg gcgccgcggc tgattgaaga tttgagctgg cctgccatgt
     4321 tggcgggcct gatagtggcg atgaaagtgt attggggtgt gctctggttc aagcccaggc
     4381 gccccggtat ccacccaact taacgagacg agtagataga tggcttctgc aaacggacct
     4441 accgccggcg aatacattgg tcaccacctg acccattggc aaaacaagcc aatgagcggt
     4501 gtcattgact tctcggtgta caacctcgac tccatcatct gggcgctgct cctgggtgtg
     4561 gttggcagct tcttcctctg gaaggctgcg cgcgcagcaa ccgccggcgt gccgggccgc
     4621 ttccaggccg ccgtcgagat cctggtcgag atggtcgaca gccaggccaa gggcatcatc
     4681 aacaacgcgc aaagccgcaa gctggtggcg cccatggcgc tgaccgtgtt tgtctggatt
     4741 ttcctgatga acgcgatgga cttgctgccc gtcgatctgc tgcccaaaat ctgggaaatg
     4801 gtgtttgcgg cgactggcca tgatccgcac catgcctaca tgcgcgtcgt gccgaccgcc
     4861 gatctttcga ccacgctggg cctgtcgatg tccgtgttgc tggtctgcat tgcctacaac
     4921 atcaagatca agggcgcggg cggctgggtg catgagctgt tcacggcacc tttcggcagc
     4981 catcccatct tgtggcccat caacttcctg atgcagatga tcgagtttgc ggcgaaaacc
     5041 gtttctcacg gcatgcgact gttcggcaac atgtatgccg gcgaactggt tttcatgctg
     5101 atcgccctga tgggtggtgc tgccgccatg tcgctgccgg gcatcctgct gccgatcggc
     5161 catgttctgg ccggcaccgt gtgggccatc ttccatattc tgatcatcac cttgcaggcc
     5221 ttcatcttca tgatgctgac cctggtgtat gtgggccagg cccacgatca tcactgagtt
     5281 tgtcagtctg gtttctcttt ctttcttttg tttgttagtt aacttaaatc cataggagct
     5341 ttaaatggaa aacgttctcg gtctcgtcgc attggccgct ggcctgatca ttggcctggg
     5401 cgctgtcggc gcatgtatcg gcatcggcat catgggcagc aagtacctcg aagcagctgc
     5461 ccgccagcct gagctgatga atgaactgca aaccaagatg ttcctgctcg caggtctgat
     5521 tgacgccgct ttcctgatcg gtgtcggtat cgcgatgatg ttcgcgtttg ccaatccctt
     5581 tgtgctgaag taatcgtcac aaccccgaca gaaaggcgtt gccgtgagta ttaatgcaac
     5641 cctgttcgtc caggcgattg ttttcgcgat cctggtgtgg ttcacaatga aatttgtgtg
     5701 gccgcccatt acgaaagcgc tggacgagcg tgcccaaaaa atcgctgaag gcctcgctgc
     5761 cgccgacaag gccaagtccg aactctccgt tgccaacaag cgcgtggaag aagagcttgc
     5821 caagtcgcgc aatgagtcgg ccgtgcgcct ggctgaagcg gagcgccgcg cccagtccat
     5881 gatcgaagag gccaaggcca aggctgcgga agaaggcaac aagatcattg ccgctgccaa
     5941 ggtcgaagcc gagcagcaaa ccgtcaaggc ccgtgagacc ctgcgcgagc aggtcgccgc
     6001 actggctgtc aagggtgccg agcagattct gcgcaaggaa gtcaatgccg gtgtgcatgc
     6061 tgacttgctg ggccgcctga agaccgaact ttgagagggg tgtcaccatg gccgaactag
     6121 ccaccattgc ccgtccttac gctgaagccc tgttcaaggc ctccgggtct gacctgggcg
     6181 gcacctcgac ctggctcgat gagctggcgg ccgtcgcgag caatgtccag ttgcagcagt
     6241 ttgccggcaa ccccagcgtg acggcgtcgc tgacgttcga cgtgatcgcc ggtgttgtca
     6301 aggcaacgtt gccggaagct gcgaaaaact tcctgcgcgc cgtgatcgac aacggccgca
     6361 tcagcgttct gccggaaatt gccagccagt tccgcgcatt gaaaaatgcg aagagcggtt
     6421 cgtccgatgc caccgtttac agcgcctttg cgcttgaggg tgcggcactg gccgatctgg
     6481 cggcgacgct ggaaaagcgc tttggccgca agctcaacct cacggtggag ctggagccgg
     6541 ccctgatcgg cggcgtgcgt gtcgtggtgg gtgacgaggt gctggacacc tcggtgaaag
     6601 cccgtttaga gcaaatgaaa gtggcgctga tttcctgagc tgacgccaag gcgcagcacc
     6661 caggttttta gccaacaaca tagaaagaag gaaagagtca tgcaactcaa tcccgcagaa
     6721 atttctgaac tgatcaagag ccgtatcgaa ggcctgactg tcagtgccga catccgtaac
     6781 cagggtaccg ttgtgtcggt gtccgacggc atcgtccgcg tccatggcct gtccgatgtg
     6841 atgcagggcg agatgctgga attcccgccc acggcggacg gaacgcccac ctacggcctg
     6901 gcgctgaacc tcgagcgcga ctcggtcggc tcggtgattc tgggtgaata cgaacacatc
     6961 gccgaaggcg acaccgtcaa gtgcacgggt cgcattctgg aagtgccggt cggtcccgag
     7021 ctgcttggcc gcgtggtcaa cgccctcggc cagcctatcg acggcaaggg cccgatcaac
     7081 gccaagatga ccgacgtgat cgaaaaggtc gcaccgggcg tgattgcccg taaatcggtt
     7141 gaccagcccc tgcagaccgg cctgaagtcc atcgactcca tggtgcccgt cggccgcggc
     7201 cagcgcgaac tgatcatcgg tgaccgccag accggtaaaa cggctgtggc gattgacgcc
     7261 atcatcaacc agaagggcca gggcgtctcc tgcgtttacg tcgcgatcgg ccagcaggcc
     7321 tcgtcgatca agaacgttgt gcgttcgctg gagcag
//
LOCUS       DAIDFG010000155         7322 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_155_length_7322_cov_17.211917, whole genome shotgun sequence.
ACCESSION   DAIDFG010000155 DAIDFG010000000
VERSION     DAIDFG010000155.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7322)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7322)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7322
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155_length_7322_cov_17.211917"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1362 a         2305 c         2252 g         1403 t
ORIGIN      
        1 tgtcctatgg cacaagcagc cccgtgccgg tggggttaaa ggcgcgtcgg ggcatccttc
       61 gacaagctca ggacgagcgg aattgcgcga acgtggactt cgatcccgac cagccggggc
      121 cgcggaaccg gcttcgccgg gccgcaggca cagcggcccc ctcggggggc agcgcagtac
      181 gcgaagtgac aagcgtgggg gccttatccc tcggtccgga cagcgtatgc ggccagggcc
      241 tgttcgtcga gcatggcgac acggccaaac tccagcgaca ccaggccttc ctgctccagc
      301 gtcttgaggg cgcggttgac cgtctgccgc gacagcccgg ccaggtggcc gagttcttcc
      361 tgcgacagta ccagccggcg ccggccctgc cagaacaccc gggtcaggta cagggccaca
      421 cgttgctccg gggagcgcag gcgctccgcc tcgatgaggg tcatggcctg gcccaggcgc
      481 cggttcaggt gcgccaccag aaagtggttg aagggcaggc tgtgggccag caagtcatca
      541 aactgggcgc gcggcaggca cagcagcagg gtgtcgcgca gggcaatcac gtcataccgg
      601 cgcggctcgg ttttcaggac cgagccctcg ccaaaccatt cgccgtccgg caccccgaga
      661 aaagccgaca cccgaccggc gcaggaggtg ctttgcagct tgaccagccc tgacagcacg
      721 gcgtaccagc ccttgaccgg cgaccccgcc gccagcatga cctcgccttt gcggcccgac
      781 atcgtgaggg tgcgctccag caggcgggtc tgggtgtcgg cgggcagcgc ggcaaaccac
      841 ggctggccgc gcagaaagcg gtggtgcggg aaaacggctt cttcgagtga catgggggcg
      901 attgtctgcg cagccgcggg gcacccccct ggttggtagg ggaatgtccg ggcgtcatgg
      961 ggtttgtacc tagaaactgt cggagcaacg acatctcttg ccggcaccgg acgcggcggt
     1021 gcacagtgcg ccatggccat tcgcgtggcg agcccggcaa ctgcccgacc gtgatgcacg
     1081 tcaagaaaaa ccgagacacc ttccctgcgc tgtcgccgag gtgctgattg aggcgctggt
     1141 ggggccggaa tccgcgtcgc gggcgttacc atgcatgcgg ttaccaggtt gcctaacggc
     1201 gcagaatttt taccggacaa gaaaggaaac aaaaccatgt tgggtttgat gcaaaaccag
     1261 ccgctgctga tttcatcgct gatcgatttt gcggagcgcc accacggcga tgcagagatt
     1321 gtttcgcgcc gtgttgaagg tgacatccac cgctacacct accgggacgt ggcgcgccgc
     1381 tcgcgccagg tggccggcgc actggacgcg ctcaagctgc agttcagcga ccgggtcgcc
     1441 acgctggcct ggaacggcta ccgccacctc gagctgtatt tcggcgtcag cggctccggc
     1501 cgtgtgctgc acacgctgaa cccgcgcctg caccctgacc tggtcgcctg gattgccaac
     1561 cacgcggaag accaggtgct gtgttttgac atgtccttcc tcccgctggt ccaggctttc
     1621 catgcgaaat gcacgaccat caagcactac atcgcgctgt gtgatgcgga caagctgccc
     1681 acggacagcg gcattcccaa cctgagcagc tacgaggcct ggatcgcccc ccactcgccg
     1741 gactacaact ggcccagttt tgacgaaaac tcggcctcga gcatgtgtta caccagcggc
     1801 accacgggca acccgaaagc ggcgctgtac agccaccgat cgaccatcct gcatgcctac
     1861 gccgcagcgt tgccggacgt gatgtgcctg agcgcacgcg actccatcct gccggtggtg
     1921 cccatgttcc acgtcaacgc ctggggcatc ccgtattcag ccgcgctgac cggtgccaag
     1981 ctggtgtttc ccggcccggc actggacggc aagtcgattt atgaactgat cgaagccgag
     2041 aaggtgtctt acgccgccgg cgtgcccacc gtctggcaga tgatgctggg ccacatgaag
     2101 cccgccggcc tgaaattctc gacgctcaag cgcacggtca ttggcggctc ggcctgtccg
     2161 cccgcgatga tccacgcctt ccaggaagac tacggcgtcg aggtgctgca cgcctggggc
     2221 atgaccgaga tgtcgccgct gggcacgctg tgtacgctga aaaacaagca cctggacatg
     2281 tcgaaagacg agcagatgaa gattcgcctg aagcaggggc gggccatcta cggcgtggac
     2341 atgaaaattg tcgatcccga gggcaaggaa atcgtctggg acggcaaggc cttcggcgac
     2401 ctgcatgtga agggcccgtg gatcgtcgcc gagtacttca agggcgaggg tggcaacccg
     2461 ctggtggacg gctggtttcc gaccggcgac gtggccacca tcgaccccga tggctacatg
     2521 cagatcaccg accgcagcaa ggacgtgatc aagtccggcg gcgagtggat cagctccatc
     2581 gacatcgaaa acatcgccgt ggcgcatcct gccgtggcca tggcggcctg cattggcgtg
     2641 aggcatccca agtgggacga gcgccccatc attgccgtgg tgaaaaagcc gggcgccgaa
     2701 gtcacccgcg aggaattgct cagtttctac gaaggcaaga cggccaagtg gcagattccc
     2761 gacgacgtcg tgtttgtcga tgccattccc ctgggtgcga ccggaaaaat gctcaagaca
     2821 cgcctgcgcg agcagctcaa ggactacacc ttgcccggcc tgtgacgcca accttgccgc
     2881 ggccggcaac cggcctggct gcgcggttca ttccgcgctg cagaatgtcc ggatttttct
     2941 gccgcggcgc tttgtcacct gcgcgatagc caggccggtt tttacgggca atcccctacg
     3001 ctgcagcgca caaaaacttg tactctgcct gtatcagtta acaaggagac atttgatgaa
     3061 attgaccatg aaaattgttg cagcgtcagc catgatgatg gttgccggtg tgtccttcgc
     3121 ccaaaagggc gagaccgtca aaatggtcag gattgacccg ctgaccgggt tgctcgggcc
     3181 ggtgggcgtc agccagatca agggctacca gttttttgcc gaaaaattca gcggcgccgg
     3241 caacccggcc ggcgtgaagt tcgaagtcac gcccatcgac aacaagctca gccccgcaga
     3301 aagcctgaac gcgctgaaag ccgccatcga ccagggcgcg cgctacatca ttcagggcaa
     3361 cggttcctcg gtcgccatgg ccctgacgga tgctgtcacc aaacacaatg agcgcaatcc
     3421 cggcaaggaa gtgctttatc tcaacgactc ggccgtggac ccggacctga ccaacagcaa
     3481 gtgcagctac tggcacttcc gctttgatgc cgacacgtcg atgaaaatgg aagccatgtc
     3541 cagtttcatg gctgaccaga aagacatcaa aaaagtctac ctgctgaacc agaactactc
     3601 ccacggccac caggtcgcca aatacgccaa ggaatccctg gcgcgcaagc gtccggatat
     3661 ccagatcgtg ggcgaagacc tgcatccgct ggcacagacg cgcgacttcg caccctacat
     3721 cgccaagatc aaggcttcgg gcgccgacac cgtgatcacc ggcaactggg gttccgacct
     3781 ctcgctgctg atcaaggccg ccaatgaaaa cggctacacc ggcaagttct tcacctacta
     3841 cgccggcgtc acgggcaccc cgaccgcgct gggcaacaac ggtgccggcc gcgtctacca
     3901 gatcgcctac aaccactaca acatgggtgg gcagatggac aagtggatga aggagttcaa
     3961 cgccaagtac aacgacgact tctacaccgg ctccaccatc cgtatcttcg agatgctggg
     4021 cgccgccatg gccaaggcca aatccactga tccggtgaaa gtggccgccg cgatggaagg
     4081 cctgaaggtc aagagcttca acggtgaagt cgaaatgcgc aagaccgacc accagttgca
     4141 gcaaccgctg tacatgtcgg tctggcaaaa ggcagacgcc aagtacccct acagccccga
     4201 gaagaccggt atgacgatgg ccccgctgaa ggaatacccc aactatgtct caagcacgcc
     4261 gaccagctgc cagatgaagc gcccgtaatc aaaagcgctt gaaacagagc ccgagcgggt
     4321 ctgccgtatc gggctctttt attttttacc gttgaggaag gtcggcagga atggagtttt
     4381 tcatcatctc gatgctgaac gggctgagct acgggctttt gctgttcatg ctcagttccg
     4441 ggctcacgct gattttcagc atgatgggcg tcctgaattt tgcgcacgcc agtttttaca
     4501 tgctgggcgc ctatgtgggc tacaccgtgg cccagttcgt cggcttctgg ccggcgctgg
     4561 tcgccgtccc cctcgtggtc ggtgccctgg gcgcattgtt tgagcgccag tttttgcgca
     4621 aggtgcacaa gttcgggcat gtgcccgagc tcttgatcac cttcggcctg tcctatgtga
     4681 ttgtcgagct ggtgcagctg atctggggcc gcattgccgt cgagttccac ccgcccgccg
     4741 tacttcaggg gcctgtcttc accctgatca acgattccgt caagggcctg agcctgatct
     4801 ggggtaccgc gcctgcggag atgtgcagcg cagccgatgc tgccgtgcgg attgtctgct
     4861 cgccgtttcc cgccacccgg ggcttcatga tgctggtcgc actggtgatg ctggcttccg
     4921 tctggctgct gctgacccgc acccgcatcg gcatggtgat ccaggccgcg ctgacacatc
     4981 cggaagcggt cgagtccctg ggccataacg tgccccgcgt gttcatgatg gtgttcggcg
     5041 cgggctccgc cctggcgggt ctggcgggcg tgatcggcgg cagcaccttt ctcacggaac
     5101 cggcgatggc cgccaccgtg ggctccgtca tcttcgtggt ggtcgtggtc ggcggcatgg
     5161 gctccctgtc gggggcgttc gtggcgtcct tgctgattgg cgtcatccag acctttgccg
     5221 tggctttcga ctattcgctg gcgaccctgg cgcaacagat cggcttgccg ctcagcgcag
     5281 gcgtggctga caactcctac atcaagctca cgctgtcgca ggtggcgccc atccttccct
     5341 acctgttcct ggtgctcatc ctgatcttca ggccccgcgg cctgctgggc aagcgggagg
     5401 gctgattcat gaaaaaccac taccaatttg cgccgctcaa catcgggcgc tggctcacct
     5461 gggggctgtt cgccctggtt ctcgccgttg cgccgctgat gtttggcagc gggctggcca
     5521 tgacggtgct gtcccagatg ggcattgcca tcatcgcctg cctgtcctac aacatgctgc
     5581 tggggcaggg cggcatgctg agtttcggcc atgcggtgta caccggcctg ggctcgtttc
     5641 tggccattca tgcgctcaac agtgtcggca acggctcctt gccgctgccg gtgtcgctgg
     5701 tgcccatggt gggcgggctg gccggcgtgg ggtttgcggt gctgttcggt tatgtcacca
     5761 cccgcaagtc cggcacgact tttgccatga tcacgctggg cctgggtgaa cttgtttttg
     5821 cgctgtcgct gatgatcccg gaattttttg gcggggaggg tggcgtgtca ggcaaccggg
     5881 tgaccggcgg tgccaggctg ggtatcacct tcggtccgca gatccaggtg tattacctga
     5941 tcgccgccta taccttcgtc gccgtcggcg ccatgttcgc cttcacgcgc accccgctgg
     6001 gccgcatgct caatgccgtg cgcgacaacc cggaacgcgt ggagtttgtg ggttacgaca
     6061 cccagaaggt ccgctacatc gccttcatca ttgccggctt ttttgccggc atctcgggcg
     6121 ggctggcagc gctcaacttc gagatcgtca ccgcggaagt ggtcggggcc gcacggtcgg
     6181 gggcctacct gctgttcacc ttcctgggtg gcgccacctt ctttttcggg cccatcattg
     6241 gcgccatcct catggtgctg gcctttgtgc tgctgtccga gttcaccaag gcctggctgc
     6301 tttatctggg cctgattttc ctgttcatgg tcatgtacgc cccgggcggc attgccagcc
     6361 tgatcatgat gaacctgcgc gtggcgatgt atggcaagct caggcagctg tgggtcagct
     6421 acctggcgct ggcggtcacc gcgctggttc tgctgctggg cgccggcgcc atgattgaaa
     6481 tggtttacca cctgcagctc aacaccacgc tggggtccca gctggaattc ctgggcgtgt
     6541 cgctggatgc caaaagcgtc aacagctggt tcggtgcggc acttgtcatg ctgaccggac
     6601 tcgggctgtt cgaggtcacg cgccggcagt tcctgcatga gtggggcgag atccaggaat
     6661 acattgaaaa agaaatcaag cgcagggaaa gtctatgagc ccccacgctt gtcacttctt
     6721 ccttccgttc gtcctgagcc tgtcgaagga tgcccccgga agtacgctga tcccgtggtg
     6781 aacggcgctc tggccctgca taaaacaata tgacctacgc acttgaactc aaagacctgc
     6841 gcaagagttt cggcaagacc gaaatcatcc gcggcgtcaa tcttgcagta accgccggcg
     6901 aacgggtcgg catcatcggc ccgaacggtg cgggtaaatc cacgctgttc aacctgatca
     6961 gcggacgctt cgcgcctacc agcggcgagg tgctgctcaa cggccagcgc attgacggca
     7021 agaagccgtt cgagatcaac cgggccggcc tgtcgcgcag cttccagatc accaacatct
     7081 tccccaagct cagcgtgttc gagaacctgc gctgcggcgt gctctggagt ctgggctaca
     7141 aatacacctt cctgaagttc ctggccgacc tcgacgatgc gaacgagcgc gccgaggaac
     7201 tcatgcagat gatcaagctc gacaagaagc gcgacgtgct ggccatcaac ctgacctacg
     7261 cggagcagcg cgcgctggag atcggcatca ccattgccgg cggcgccaac gtcatcctgc
     7321 tc
//
LOCUS       DAIDFG010000156         7314 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_156_length_7314_cov_17.845433, whole genome shotgun sequence.
ACCESSION   DAIDFG010000156 DAIDFG010000000
VERSION     DAIDFG010000156.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7314)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7314)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7314
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156_length_7314_cov_17.845433"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1259 a         2306 c         2427 g         1322 t
ORIGIN      
        1 tgcgggccag cacgtcggcc ctgccggttg atcactactg cgagtctcgt gctccatcaa
       61 gaacaaacag gatccgaact gcgcaagaga catgacgcca ccaggcaagg cacggccatt
      121 gggaacaaac catccgagac ctgaaggttc tattgtgacc gtgaggtaga aaataaagcg
      181 gctactaccg attcagacgc gagcggctaa aatatcggca tgagcaccac cccttctacc
      241 gcgccggccc tcaaggccag acccgccgtg catggcggcc ccaaaatctc tttcaaggcg
      301 caaatgctgg ccgcccggga ggacgccatt gtgcaggcgg tcaaccggct gctggcggaa
      361 aaaggctttg acctgatgac ggtggacgag gtggcggccg atgtgggcat tgccaaggcc
      421 agtctttaca agcatttccc cagcaaggaa gacctggccg cggcggccat ggtacgtgtg
      481 gtggggctgg cgcaggcctt tctggccagc ttgcccccgg acgcccagcc cctggcgcag
      541 ctcaaggcga tggtgcgctg gatgcttcaa ttgcagctgg ccggcgaaat gccttcgctg
      601 ccgcaccaga actccagcct gcgtaccacg ctgatgaaca acaaggccta cctcgacgga
      661 ctggtgcttg tcagcgacca gatcggcggc tggatagaac gtgcgcaaaa agacggatcc
      721 ctgagcgccg acattccacc gatcatggtg ctctacacct tgtacgcccg cgcctgcgac
      781 ccggtgctgg agtttctgcg ggccggcggc cagcacagcg atgaagagat cattgagctg
      841 gtgattcgct cgtgcttcga ggggctttgc gcgcgttaaa aacctgcaaa tccgactgtc
      901 ctggcagtat tcgcaaaagc cgggatttgc attacattgt gtaactcttt tgtgtctgtt
      961 gctccccgtg ggtcagcctt cctgccgggg gaaaccccgt tccaacccat gaagtcgccc
     1021 gctttgcgca tgaacccggc cagatcggcg tccgacaggc tcctgggcgc ccgcgcctcg
     1081 cgggtgtggc cggaccggcc atgacccgtc agctggcgtc ttcggattcc cagtccatcc
     1141 gaagccggcg cctggcgatg gctatggcgg gactggccct ggtgcttggc ctgcttttta
     1201 ccggggctgg cgttttctgg gtgaacaaca cgtttcagag ccaggccatg gagcatttcg
     1261 accggctggc cgaccgtgtc caggccgatg tgcaggaccg cctgaacgcc ccgttgctcg
     1321 gtctgcgcgg cgccgccggt gtgtatgcgg ccagcaactc ggtcgagcgg gccgagtttc
     1381 gcgcctatgt gcagttcagc caggtggggc gcgatttccc gggcgtgcgc ggattcggtt
     1441 tcatggcgcg ggtcctgcgg gaggatctgg atcgtttcgt ggccgccgaa cgtgccgacg
     1501 aggagcccgc ctttgccgtc accgcgatgc ccggctcgcc gaccaccagc agtgcgcccg
     1561 acctgtatgt ggtgaagttc atggaacccc aggcccggaa ccagtcggcg ctgggcctgg
     1621 acgtcggggc agacccggta cgccgcgagg ccatcgaacg tgcggtggcg acgggcgagc
     1681 ctaccctgac ccgaaaaacc accctgctgc aggaccagca gcaaagcccg gggtgggttt
     1741 acctgttgcc ggtttaccgc acgggggagg accccagcac gccagtgcaa cgccaggcgc
     1801 tcctgaccgg ggtgctgttt gcgcccatgc tggtcagcga ggtcttgcag ggggcggccg
     1861 aggccaccca ggggcaggca gatttcgagc tttttgatgg cgaatcgctg aacgcacaga
     1921 cgctggtgta cggtctcgac gggcatctgg cggcgggccg gagggacccc gtgtcggacc
     1981 acgccgccca gcatcgtttt tctacttccc ggccggtttt ggccggtggc cgcaccctga
     2041 ccctgcgtgt cagcaccacg cctgcgtttg aagccgaggc gaccagcctc atgccggcct
     2101 tgctcggact gagcgggggc ctgctgagcc tgctgctggc cttgtgcctc tggctgctcg
     2161 gcagcagccg ggcccgggcg ctgtccgtgg cccagcgcat gacgctggac ctggcgcacg
     2221 agcagcagcg cctgctcagc atcgtggagg ggaccaatgt cggcacctgg gagtggaatg
     2281 tgcagaccgg ggaaacccgc ttcgacgaac gctgggctgc catcaccggc cacacgctcg
     2341 atgggatgcg gcccctgaac atccagacct ggcgcgacct gactcatccc ggggacctgc
     2401 tccacgccga tggactgctc aagcagcatt ttgccggcag gacgccttac tttgactgcg
     2461 agctgcggct gcgccacaag gacggccgct ggatctgggt gctgtcgcgc ggccgcgtct
     2521 gtgcatgggg cggcgaccgc cagccggcgc tgatggcggg taccctgatg gacatcaccg
     2581 aacgccagac cgcccagctg gctctgcgca acagcgagga gcattttcgc cagctgtttg
     2641 aaaccagcct ggagtgcatt ttgcaaaccc ggccggatgg acaggtgctc aacgccaacg
     2701 ccgccgcctg tgagctgttt ggcatgagtg tgggggagtt gcgcgagcgc ggccggggcg
     2761 ggctggtcga ccccggcgac acacgcttgc aggccctgct cgacgaacgc cagcgtgaag
     2821 gaaaaacgcg cggcgagctg cgcatgttgc gcggtgacgg aaccgccttc gagtgcgagc
     2881 tgtcgtcgtc gatcttcctg gattcgaaca acgcgccctg cgccaacatt atcttgcgcg
     2941 acatcagcga gcgcaagcgt tcggaagcgc gcatcgcact ggtcaacgcc gagctggagc
     3001 aacgggtggg gcggcggacc gcccagctcg aagcgtccaa ccgggatttg caggagtttg
     3061 cccactcggt ggcccatgac ctgcgccagc cctttattgc gattggcggc ttcagcggtt
     3121 tgcttgagcg ccgggtgacg gacgaccgcg ccaggcatta catcaaccgc atcaaggccg
     3181 gcgtgaggca ggccggggag ctgacagaag ccctgctggc gctggccaac ctgtcgcgcg
     3241 tgcagctgcg cgtgcaggcg gtggacctca gtgccattgc gcacagtgtg atggacaccc
     3301 tgcagctgga ggagcccgcg cgcgcgcgct gcatccgcat ccaggagggc ctggtggtgc
     3361 aggccgacgc gatgctgctg cggctggtga tgcaggagct gctcggcaat gcctggaaat
     3421 tcacgtcgcg gcagacacac accgaggtca gttttggcct gcagccggcg ccggcaacag
     3481 gcacgcaagc cgtctacgtg gtcagcgaca acggcgaggg cttcgacatg gcccatgtcg
     3541 acaaactgtt ccgcagcttc cagcgcctgc acgccccgca ggacttcccc ggcgcggggg
     3601 tggggctggc caacatccag cgcatcattg cgcgccacgg cgggcagatc tgggcagagt
     3661 cggcccgcgg cgcgggggcc cgcttctttt tcacgctggg cagccccggg atgtgagttt
     3721 ttttttgggg catcgctatt gttttgatag ctgtttgcgc ccgccagata tgggctatac
     3781 cccgattttg atcaacaaat cgggtttgaa tccatcctgt tcacccttgc gggcataacc
     3841 gagcggattg gccaccaccc ggcagccgtt gctgacatag tcgttggggc agtgcaggtg
     3901 gccgtgcagc cacagctgcg cctgcgccag cagatcgtcc agcgcattgc agaaccccgc
     3961 cgtaccgggc gccaggccgt agcgcgggtc gccgctgagc aggctgggtg caaagtgcgt
     4021 gacgaccacg gtcgggccgt caaaggggat cgccagcgcc tgccttagcc aggcctggct
     4081 ttgcaggccc agttcgcgca cggcgtccgc cagcatgggc tggccatccc tgaaggcgtg
     4141 gtttttgcgg aggtaaaaat tggcggcgcg aaaggccttg tcacgcgcct tgaggacatc
     4201 gcccgtggtc aggctgccgt cggccgcctg tgtggcggtc agcgcatcaa agtcggtcca
     4261 caaggtgcag ccgacaaaac ggacgccccg ccactgcaca gtctcgcgtt ccagccagac
     4321 caggcccagc cgctcgcagg tctcgcgcag gcgcgtgtgc gtggcctcga agtcctgccc
     4381 gtcgtactcg tggttgccgg gcaggaaaaa agccggcgtc ggccaggccg cgccgccctg
     4441 cgccaccggc aggggagaaa agcgggcgag tccgaagtcg tcgtcatcca gcatgtcggt
     4501 cagcagtgat cccggctggt aggaaccgat atcgcctgcc agcaccagca ggtccgcccc
     4561 cgggacgggc tgggccacga aatgcggatt ggattcaagg tgcaggtcgg acagaagctg
     4621 gatattcacc ctgccgattg tgcaggaagc ctgtttcgcc aacggttggc ggcattttcc
     4681 aacccgggaa gaggtcaggg agcccccgga gagcggaaac gcccgtctat gccgactggc
     4741 aggccaacta tgcgccttat gcatgggtat cggcctgttt tgggcttgca gccctaggga
     4801 aaacccttaa tgtagaggta tcgcaatttt tcgccacggg atggcgccaa gacatgtaca
     4861 cccttctttc gcaggttttc cgttttcttc catttccccg cgccgtggcg ccggttcacg
     4921 gcgtggcaca caacctgatg gaacgtgccg aagcccgggc cggccgcaac ccgcagcagg
     4981 cccaggaact gcgccgtgcg gcgcgcgctt atctcagcgt ggtccgctga tacgccctga
     5041 aggttcacgc cggcgacccg ttgtggccgc aggtttggcc gggtgatgcc ccgtccggca
     5101 gcgtcagcct gaaaaggcct tgtcggcggc gcgcaccagg gcctggcgca gccagacatg
     5161 ggcgccgtgc tgctgggcgc ggcggtgcca tatggcatcg acatgcaccg gcgacacgtc
     5221 aaagggcagg ggtcgcaaca ccagctgttc ggcgatgccc gtgacccgca caaagtggcg
     5281 gggcagcacg gtgaggaggt tggagttggc gaccacccgg ccggccgtga agaactggtt
     5341 gactgtcagc accaccttgc gttcgcggcc cagcgacgcc agtgattcgt cgataaaccc
     5401 gtaagggcgg ccggaaaaac tcaccagcat gtggcgcgca gcgcaatacc ggttcagcgt
     5461 gaaagggccg ctagccagcg gatggccggc gcgcatcaca cacacatatt cgccgtcata
     5521 cagccgctgg tgcaaaaaag gcatgacctc gccgctctgg gcgcgggccg tcaggtccgc
     5581 cagcacggag gggaagtagc cgatcgccag gtcgcaggct tcctcgtcaa ggagtttgcg
     5641 cggatcgcgc gttgtcagcg gcaccacgcg gatggacacc cccggcgcct catgctccat
     5701 gatgtccacc agccccggta tcaactcggc ggccgttgcg tcggccatcg ccagcacaaa
     5761 cgtggaatgg gcttcgccgg gcacaaacgt gctgggaatc agcgactcct gcaactgttg
     5821 cagcgcctcg cgcacggccg gccagatcgc gagtgcgcgc ggcgtgggcg ccatgccctg
     5881 gccgctgcgc tgcaccagct cgtcgcccag cgtctcgcgc aggcggcgca gcgcattgct
     5941 gacggcgggc tgggtcagcg acaggttgcg cgccgcccgc gtgaggctgc gctcggccat
     6001 gacctcgtca aacacgcgaa gcaggttcag gtccagggta cggaagttcg gtggagtcac
     6061 gatttttcct gattcagcgc gagttatcac tgttgtgaat agattgcatc ataaagataa
     6121 agttgaataa cattagcgtt aaccctaata tccgtctgtg cccaaaccaa taccggtggt
     6181 ggcactcatc aggggaacca tcatgaccag ttttgtcaac atcaaatatc ccgccgaaca
     6241 tcccggtgtg gcacgttttg agtcggccgt gggtgcggct cgccaactgc gcaacggctt
     6301 tgacagcacc cggggcttgg cgggtctgct gctggcagcc atcgttgcgg ccatggtggt
     6361 ggtggctgac cagctcgtcg agaactgggc cgaaggccat ttgctggtgg cttgggttgc
     6421 catgtgggcg gttgccttct ccgtgatcgc cctttttgcc gggacggccc gccacttggc
     6481 ggcgcgtgtg ctcgcggcac tggacgcctg gtccagcgcc attgcccagg cgcgtgccga
     6541 tgtccggctg tgggaagccg ccaaggcgga cccccgtgtg atggccgacc tgcaggtcgc
     6601 catggcgcgc caggaagtct cggaagaagt ggctggcgtg ccgcctgtgg ttgccgtggc
     6661 agcggccaag gcgcgggaag acaaacccat cgacttgggt agccgcgtgt tgcgcgcata
     6721 ccccagctac tacatctgag ttgccgggcg cgcttaccgc gcctgcgttg tcacaaaaaa
     6781 agccacccgg tcgggtggct tttttgcgtc cgggcggctt gcccgacagg ctcttggggg
     6841 cgcaacgcag atcatgcgcc gcgcccggac tcgtccgggg gccggtcagg ccgccagacg
     6901 gcgctcaatc gcggctttgg tctcccccag ctctttcggc aggtgatgtt cgagttgttt
     6961 gaacaggtcg gtgtgcagtt tcagctcttc agtccaggcg gccttgtcga tgctggtcac
     7021 ggtgttgaac tgctgggcgc tgaagtccag gccggtccag ttgatttcgg cgtattgcgg
     7081 tgcaatgccg aaaatggtgt cctggccctt ggcctggttt tcgaggcggt cgatcatcca
     7141 cttgagcaca cgcatgttct cgccataccc cggccagaca aacttgccgg cctcgtcctt
     7201 gcggaaccag ttcacgcaga agatcttggg cagggtgtgg cccaggcttt cgagcttgtg
     7261 ctccatgtcc agccagtgct ggaagtagtc gctcatgttg tagccgcaga aagg
//
LOCUS       DAIDFG010000157         7310 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_157_length_7310_cov_19.059132, whole genome shotgun sequence.
ACCESSION   DAIDFG010000157 DAIDFG010000000
VERSION     DAIDFG010000157.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7310)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7310
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157_length_7310_cov_19.059132"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1254 a         2440 c         2428 g         1188 t
ORIGIN      
        1 gcagggagct acacgaagtg agcgaccgtg ggggctctaa aatactgcat gactttcctc
       61 gagatgctgg gcgcggctga gcgccagaac caatcgatgc tgtgtgtggg cctggacccg
      121 gagcccacga agttcccggg caagctcaag ggcgactcca acaagatcta cgacttctgc
      181 gcggccattg tggacgccac ggccgatctg gccatcgcct tcaagccgca gatcgcctac
      241 tttgccgcgc accgcgccga aggccagctc gaacgcctga tggaacacat gcgccgcgtg
      301 gcaccgcagg tgcccatcat cctggacgcc aaacgcggtg acatcggcag caccgccgag
      361 cagtacgcgg tcgaggcctt cgagcgttac ggcgccgacg cggtgaccct ctcgcccttc
      421 atggggtttg actcggtcgc gccctatctc aagcacgccg gcaagggcgc atttttgctg
      481 tgccgcacct ccaaccccgg cggggacgac ctgcagaacc agcgcctggc ctccatcgaa
      541 gggcagccgt tgctttacga acacgtggcc cggctcgcgc agggcccgtg gaacctcaac
      601 ggccagctgg gcctggtggt gggcgccacc tacccggcag agatcgaacg tgtgcgcgcg
      661 gtggcgccga ccttgccgct gctgattccc ggtgtcggtg cacagggcgg cgacgccgtg
      721 gcgaccgtga aagccggctg gcgacccggc gcgcccatca tcgtgaactc ctcacgcgcc
      781 atcatttacg cctcgtcggg tgacgacttt gccgaggccg cgcggcgtga agccctccgg
      841 acgcgggacg tgctgcaggc cgcgcgaagc tgagcttctg gtagcagccc agccaccact
      901 cccgggtcag cgatgcaact caaatggctg gaagacttcg tggcgttggc gcaaacccgc
      961 agcttcagcc gcgcggccga gttgcgccac gtcacgcacc cagccttcgg gcgccgcatc
     1021 aaggcgctgg aggcctgggc cggcacgacg ctgatcgagc gcgagaaaac cccgctgaca
     1081 ctcactgccg ccggcaccag cctgttggac aacgcgcagc agatggtggg caacctgaag
     1141 tccgcgcacg acgagctgct caatgccgcc ggacgccacg agaacctggt cgcgctcgcc
     1201 accgggcgca cgctggcgcg caccctggcg gccgatctgc tgctgcgttt caggccgctg
     1261 ctgggggagg gcgagctgcg tatcctgacc cgttccctga ccgacaccgc gcagatgctg
     1321 gaacgcggcg aggtcgactt catgctgatt taccatcacc cgctgctggc cgtgcgcctc
     1381 agtgcgcgcg agtacgcgca cctgacgctg gcccgggacc ggctggtgcc ggtcacccgg
     1441 gccaatgccc aggggcgcgg gcagcaccgc ttcggcgcaa gggccgacac gccctacctg
     1501 gcctatgcca gcaccctggc gctgggccgg ctggtgcagg accatctggc caacaacccg
     1561 catgctccgc gcctgcagcg ggtgatcgag tgcgactcgg ccgacgccgt gtacgaatat
     1621 gccctcaagg gcctgggggt ggcctggctg ccgtggtcca tggtgtcggc tgcctgcaag
     1681 aagggccagc tggtggtgct gggcgacgcg cacctggagg tcggcttcga agtgcgggct
     1741 taccgcgcca aacaccgcct gaacgcgctg gcagaacgtc tgtggcaggt gatggcccag
     1801 cgctgagctg ttgccaaaac agccatgaac ggtgccgaaa cggcaccaaa cggaaagccg
     1861 ccatcaagac aatgaaaagg tctgtgtcct ttcaggagtc gcgatgtccg cccatgctac
     1921 gccaggatca ccgaataacc tcccttcccc tgattgttga aagaaaaacc atgtcgttcc
     1981 tgcctctttc gcgccgtacc gccgtttgcg ctgccctggc tttgctggcc tgcgccagcg
     2041 tgagcgccca gcctgacagc tatcccagca aacccatcac catcgtggtg gcctacccgc
     2101 ctggtggcag caccgacctc actggccgca ccctgggcgc cgaactgtcg cgcaagctgg
     2161 gcgtgccggt gattattgac aacgtgggcg gtgccggtgg cgcgatcggc gcgcagaagg
     2221 tcgccaatgc cgcaccggac ggctacacgc tgctggtcgg cgccagcaat gaaatcgcca
     2281 tcacccggct ggtgtcgagc gcagtcaagt acgacgtcaa ggacttcacg ccgatcggcc
     2341 tgattgccac acagcccatg gtgctggtgg cctcgcagaa gtcgggcgtc aagacggtgg
     2401 accagttcat gagcctggtc cggcaaaacc ccggcaagta cagttttggc agctccggcg
     2461 tgggcaccgc cctgcatctg gccggcgaga tggtcaagga ccagggcggc ctcttcatga
     2521 cccacattcc ctaccgcggc gtggcgcccc tggccaacga tctgctgggc aacaacattg
     2581 acttcggtgt gttcgtgctg tccagcggcc tgccgcacat ccgcagcggc aaggtggtcg
     2641 cgctcggcac caccgaagcc cggcgctcgc cgatgactgc cgacatcccg gccctggccg
     2701 agacgcccag gctcaagggc atcgacatca acatctggtt tgcgctgatg ggaccggccc
     2761 gcctgcccga gccggtggtg gccaagctca agaaagccct gaacgattcc ctgcagacgc
     2821 ccgagctgcg caagaagctg gaagattcgg gctcgacggt ttcgcccctc aatgtggaca
     2881 tgccccggtt cctgagccag gaaaccacca aatacaagcg cattgtcgag tttgcgaaca
     2941 tcaaagaatg agcaccgcct tgccatttga tttgcccaag ccggacatcg ccgcctggcg
     3001 cgcgggcaac accggcaccg aaggtgtctg gcagtttgat tcgggcctgc cgggcaggca
     3061 ggtgctgatc accgccctga tccacggcaa cgagctgtgc ggcgcctggg cgctcaaggg
     3121 cctgctcgag gccggcgtac ggccgaagca ggggcgtctg acgctggcgt tctgcaacct
     3181 ggccgcgttc gaccgcttcg atgcggccaa ccacgatgcc tcccgcttcg tggacgagga
     3241 cctcaaccgc caatggtcgc ccgagcgcat gcacgtcggt ggcacacagg agcgccggcg
     3301 tgcggcggcc ttgctgccct ttgtcgagca ggccgactgg ctgctggatc tgcactccat
     3361 gcacgaacgc gccgccccct tgctgctggc cggcatgcag ccacgcaacc tggcgctggc
     3421 ccgccgtctg cgtgcgcctg aacacgtggt ggtggatgcc gggcacaagg acggcgtgcg
     3481 catgcgcgac ttcggccgct ttggagaact caacccgcag ggcgaggacg cggcctgttc
     3541 actcctgctg gaatgcggct ttcatggcga cctgggcagc cgcgtggtgg cccaggacat
     3601 gtgtgtgcgt ttcctgctgg ccgccggggt gctggacgag gcgaccctgc aacgcgccct
     3661 gccgggctgg cagcaaggag atgccccgcg ccagtgggtg ctggaagtca caggcccggt
     3721 ggtggcgtgc aacgaccggt tccgttttac ccacgccttc caggggctgg agctgataga
     3781 acacgccggc accgtgattg gctggaacga cagcgagcct gtgagcacgc cctacgacga
     3841 ctgtgtgctg gtgatgcctt cggtgcgcca agcccgcgcc ggtgtcaccg tggttcgttt
     3901 cgcgcgccgc cggcctttgt aaaaagcctc gctgcgttgc tgcaggtttt tcgcaggcgt
     3961 tgcagcgggc tggtttgcta tattttcagt agctgtcggc gcccgtgttt aaagggctgg
     4021 aggccgattt ggttcaatga gcgacggtaa accgcttctg cacatgccgc ggcgtttcga
     4081 tttcatcaac gatggccacg gccaggtcgg ccactgaaat gccggccggc tggtcgcctt
     4141 ggccgggcag caggtcgtcg gcgccgacgc ggtacttccc ggtgcgctcg cccggtgcca
     4201 gggcaatcgg cggcgagaca aaactccagt ccagcgtggt ttctttcttg agttcgttca
     4261 gcgcctcgcg ggccgccagc gcgccctgtt tgtactgggc cggaaactcg ggcgtgtcca
     4321 ccagctgcag accgggggcg acatacaggc tgccggcgcc gccgaccacc agcaggcgtt
     4381 tcaggcctgc tgccttgacg ccctgcacaa tggcccggct ggcctgcagg tgcagggcgt
     4441 ggatggccgg ctcgctccag ccggggttgt aggcgctgat caccgcctca tggccggcga
     4501 cggcttgggc gacctgcttg gcgtcgagcg cgtcggcctg caccacggtc agcccggttt
     4561 gcgcggggag tctggacggc gtgcgggcca gcacggtgac ctggtggcca cgacccagca
     4621 actcggaaag gatgggggag ccgacgaaac cagtggcgcc tatcaatgcg atcttcatga
     4681 aaaactccag agtgattgaa gaagcctgta gtctgatttc ttcataaaca attgaatagc
     4741 cggcaaaaat cgctaatact gtgaagctca actaaccaat agagccaaaa ctggacccct
     4801 ataaacgcat ggcgattttt gccgaggtgg tggccgcggg ctcgctgacg gcggcggcgc
     4861 gccagctggg catgacaccg tcggcggtca gccagcattt gcgccagctc gaggagtcgc
     4921 tgggtctggc cctgctgcac cgctccaccc ggcggctcac gctgaccgaa gccggtgagc
     4981 gttaccacac gggttgtgcg gccatggtcg ccgcggcgcg ctcggccgac caggccctgg
     5041 cgcgtctgcg tgacgagccc gaaggcgagt tgcggctggc ggcccccatc ggttttggcg
     5101 ggctgctcgc cacggcgctt gcgcccctgc gtgcccacac gaagctgtcg ctgcacctgc
     5161 tgctggatga cgcggtgatc gacctgattg ccgcccgcgt ggacatcgcc ctgcgtgtgg
     5221 gtgcgctggc ggattcaacg ctggtggcgc gcaagctcgg cagcatgaag cggcaactgt
     5281 gcgcatcgcc ggcctatctg gccgggcggg gctggccggc gcagccgcaa gacctgctgc
     5341 aacacgactg gctgggcggc cggccgggcg cggccggcgg cgagctgctg gacttgcagg
     5401 gcccggacgg cgagcaccag acggtgcgca tgcaggggcg cgtgcaggcc acgcaggtga
     5461 cggccctgca cgccttgtgt gtggcgggct ggggcatcag cattgtggtc agcgacgatg
     5521 accgccgtgc gctggccgac gggcgactcc tgccggtgct gcccggttgg caactggctg
     5581 acctgccggt gtacgcggtg acgccgcggc gcggcgagca gcccgccaag gtacgccatg
     5641 cgctggagct gctggccgcg tatttcaatc agccagccgc agggccgacg cgtctcagcg
     5701 cggcttgagc atcccgccca gcatcacgca ggccagcgcc gccaccacca gcgtgatgcc
     5761 ggcccactgc cagggcgtga tggcttcacc gagcaccagc atgccggtgg tcaggcccac
     5821 caccggcacg cccaggctga agggcgcaac gcggttggcg gggtggcgct tgagcaggcc
     5881 ggtccacagg ctgtaaccca ggatggtcgc gacccagccg agataggcca ccgccagcca
     5941 gctggaggcg cgggcctggg tccactgcca gcgcgtcgcc tcggggtcga acaccagcga
     6001 cagcagcgtg aagggcagga tggcgaagat gctgctccag accacaaagg ccatcggcga
     6061 gtaggctacc cctccttgct gcacgtggcg cgccacgatg ttggacatgg cccacatggc
     6121 ggcggcgccc agcgtgagca caaaacccgc cagcgtggtg gcggcaaccg cgccgccgtc
     6181 ggcgggtgcc aggtaattca gcgcaaagca ggccagaccc agcgccgcga gcaccatgcc
     6241 ggcctgcaac ggccggctgg cgcgctcgtg cagcagcaca aagccgaaca gcgcggtgaa
     6301 aaacacctgg gtttgcagca gcaccgacgc cagcgccgcc gtcatgccga ctttcagcgc
     6361 gacgaacagg atgccgaact gcccgacacc ctggaacagg ccgtaaaacg ccagccattt
     6421 ccacgggata ttgggcggcc ggacaaacaa caccagcggc agcacggcaa acacataacg
     6481 tgcggcgccc agctggaagg gcgtgaagtc gcgcagcgcg aatttcatga ccacaaagtt
     6541 gacgccccag accacgacca ccacgagggc ggccgccaga tcgcggccgg tcaggacgcc
     6601 gggccgcatc aaacgcccgg cagcgcggcg cgtgcggcgt tcaggtgggt cctgacgtgc
     6661 tgcgccaggg cgatgtcgcc cggtgtttcg ggcaaccagg cctcgacagg aatggttttc
     6721 ccgttcgggt ccaccgcgac aaacaccacc acacattcgg tgatgacttc catctcgccg
     6781 gttttcatgt cgccggcgcg cacctcgacc gcgatgttca tgctggtgct gccggtgtag
     6841 gccagccgag cctgcacttc caccaggtcg ccgatcatga tgggacgctg gaagcggatg
     6901 ctgccgaccg acaccgtgac gcagtaacgc ttggcccagc gcgtggcgca ggcgtagccg
     6961 gcttcgtcga tccacttcat gacggtgccg ccatggacct tgccgccgaa gttgatggtg
     7021 ctcggttcgg ccagaaaacg cagggtgatc gacgacatac ggctccttgt gaaatgctat
     7081 gaaaataata gctgtttgcg cccgtccatc aagggctgca gccagtttct ttacaaaagt
     7141 cgcttcaggt aacgcccggt atggctggcc gggttggcgg cgatgtcttc gggtgtgccc
     7201 acgcccacca cggtgccgcc accggccccg ccctcggggc ccatgtcgat cagccagtcg
     7261 gcggtcttga tgacgtcgag gttatgttcg atcaccacga tggtgttgcc
//
LOCUS       DAIDFG010000158         7301 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_158_length_7301_cov_17.436655, whole genome shotgun sequence.
ACCESSION   DAIDFG010000158 DAIDFG010000000
VERSION     DAIDFG010000158.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7301)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7301
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158_length_7301_cov_17.436655"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1294 a         2236 c         2285 g         1486 t
ORIGIN      
        1 cgtggcgctg tccagcttga agtcaaaggc cagggtgtag gcgtccttga cttcctgcgg
       61 gcccttcttg ccgatgctga cttccagtcc cgtggggctg ccgttgccgc cggtggtgcg
      121 gcagaacttg gtgttgaggt gcagcatcag cacatcgcac cagttgtcgg ggcttttgaa
      181 agccgtggtg accgctgaaa acgggtagtt gatgaccgca taggcattgc cgctcacggc
      241 attggcggct tcggtggatt cgatgaccag cggcctttga aactcgttgc gcgccagttg
      301 gggggcgagg gcggtgtatt tggcctgcag cgccgcggcg cccgagtcgc tcgacgtagc
      361 catggcgctg ccggcaaggg cagtggcagc catcaggcct gcggcggctt ggcgggaaat
      421 ggattttttg tgtgcttgag tcatcgtgac agtttgcctt ccgcagggcc tcttgttggc
      481 gacttgctcc ttcgtcctac acgagttgag tgcgagaagg aaatcaacag cagcttcaag
      541 aaaaaagtgc ttttactatg tttcggcgtt cagaccgaca acacgtaatt gggggcatgc
      601 ctgcaaggtt caacccgcca tttgtgacat gcggcttttc cagtgcgcga taacaagatt
      661 gctaccccgt cgaaacttgt gcttgggact ttcatcatta atgtgattta ttttgtagcg
      721 ccctgacaac gaacctgtca gttctgacag agcgaggttc atcgcatcat caacgactgc
      781 cacgccgagg ccaggcgcct gctgacccag cgccgcctgc agctcgccgc gctggtcgag
      841 cacgaaacgc tcggccagaa aggaatcctg aaggtcaccg gcctgccgcc ggcgcccgaa
      901 ctggagtacc ggccgctggc ggctgttgca gcgggattgg tgacctccat gaactgggcg
      961 tgcggcgcag accggcgctt tggccggtct gcttcagctt cgctgcggtg ccagtgtctt
     1021 ggtgtagagc gacgcgccgg ccagatcttt caatgtgact gtcatggctc gggttttggc
     1081 gtcgatgtcc acctgaccaa aaaactgcat gccgttggtg ggtggtgcat tcacttcgtt
     1141 cggggctttc tggtacatca cctgcatgcc aaaggtcgcg tcggttttgt tgggcccgaa
     1201 accaccggcg ttgagcggcc ccgacacaaa ttcccaaaac ggcgagaagt cattgatcct
     1261 ggccttggcc ggatcaaaat aatgcgcggc ggtgtagtgc acatcggcag tcagccacac
     1321 cacattcttg atgccggcgc gtttgatctc cctcagcagg cgcgcaattt caacttcgcg
     1381 gcccatgggg gcaccgtcgt cgccgttggc accggcttcc cagcgggcgc gcccgtcggc
     1441 gtctttgcca tcagccacat gcaggctgat gggcatgtcg gcggcaatca ctttccagac
     1501 tgacttcgaa cgcttgaggc cgtccagcag ccaggcaatc tgggggcggc ccatgaaggc
     1561 gctttcttcg gtctcgctgg tctgcagatt gtgggtgttg gggccacggt agctgcgcat
     1621 gtcgaccaca aacatgtcca gtagggggcc ctgcggcaga tgacggtaca cacgctcgct
     1681 ttccacgtcg gcggtgcggc gcaggggcgc aaattcatga aaggctttgg tggcgcgcgc
     1741 caccagcagg ggcacgttct tttcggtgta acgcttgtcg gcagcgaggt ctttggagtc
     1801 cgaccagttg ttgaccacct cgtggtcgtc ccattgccag atctgcggca cttccgccgc
     1861 catgcggcgc acattggcgt ccatcaggtt gtagcgatag cggccacggt actcattgag
     1921 tgtttctgcc accttgctga cctcttcggt gaccacgttg ttccacaggg agccgtcagc
     1981 gagtttgacc tgggcctgga tcgggccatc ggcgtaaatc gtgtcgccgc aatgcaggaa
     2041 aaagtcaggg ctgaccttgc gcatctgctc gtagattttc atgccgcccc aggcctcgtt
     2101 gatgccccag ccttgccccg ccgtgtcgcc gctccaggta aaacgcacgt cgcgcgcagc
     2161 cttggctcct gcgccggcgg gcaggcgcag gtggccggcc atgggttcag acagcacacg
     2221 ctcgttgtgc aggtcctgca gcaccacccg gtaaaaaatt tcctggccgg ctggcaggcc
     2281 cgccaggtcc acacgaccgg tgaagtcggt gtcgcccatc aggtagggcc cacgcacccg
     2341 cgtggcgttg gcaaaactgg cggtcgttgc ccactccagc caaaggcgcg caggccggtc
     2401 gctacgggtc cagatgatgg cgccgtcgga cttgggatcg ccgctttgaa tgccatgagc
     2461 catctggggc cgcatggcgt ctcgtgtgat gacggcgggg gcggtctgcg cccgggcgag
     2521 gcggggcagc gccagggccg aggttcccaa aacggtgctt gaattcaaga atgtgcggcg
     2581 atccatgaag attctccaga agtaaaacgt agaagctcgt actgttttgg accaacatgt
     2641 cagcgccatg aaacttttat tgaagaacgc ccggtgttgc cgccgcaccg cacgtcccca
     2701 cctcccgctc actgaaccgc cggggttgca gcaatccatc agatcgcacc aaagtgatta
     2761 acggatggca ggaacgcact gccttggggc aacgcggtgt atggattccc cgccggtggg
     2821 cggattggcc ggcgccacgc ttcattttgg tggcacagta tgtgcttgtt cctgggtgac
     2881 tcaggctaac agcggcctga tcaaggcgcg gcaagtcgct gcgcactgat tcaaacggat
     2941 aacggcgtcc acttctgtct catcttttga ggcattggaa gtggacgttt ttttttgtct
     3001 tgtttgtctt gttactacgg accaaggaaa ttgcatgtct tatttagtgc cttcagagtt
     3061 tgtgacgaag atggtggatg ccggtgaatc caaaatcttc atgtccaccc gcgacaccgt
     3121 gatccgggcc tacatggccg gtgccatcct ggcgctggca gcctggttcg ccgtcaccat
     3181 caacgtccag accggccagc cgctgctggg tgcggtgctg ttcccggtgg gcttcgtcat
     3241 gctctacctg ttcggcttcg acctgctgac cggcgtgttc gtgctgtgcc cgctggcact
     3301 gatcgacaag cgcccgggcg tcacgctcaa gggcgtgctg cgcaactggg gcctggtgtt
     3361 cgtcggcaac ttcgcgggcg ctttcacggt ggcggtgatg atggcgatct acgtcaccaa
     3421 cggcttttcc acaccgccca gcgcggtggg cgtcgccata ggcgtggtcg gcgagaaccg
     3481 cacgctgggt taccaggcgc atggcgccaa cggcatgctc acactgttcg tgcgcggcat
     3541 gctctgcaac tggatggtgt cggccggcgt ggtgggggcg atgatctcca cccaggtcag
     3601 cggcaaggtg atcgcgatgt ggatgcccat catgctgttc ttctacatgg tgttcgagca
     3661 ctccatcgtg aacatgttcc tgttcccgtc cggactgttg ctgggcgcga agttcaccat
     3721 catggactac ctgatctgga acgaaatccc gacggtgctg ggcaacctgg tgggcggcct
     3781 ggccttcacc ggcctgacgc tgtatgccac gcacatcaaa accggaccga cacgttcctt
     3841 caaataatcc gtctcaacaa caaaagccct cgcgcacgca ggatgcgcgg gggctttttc
     3901 ggtaacctcc atgtccagtg aactgaagat atcggcaggt cagtattcgg acaagggccg
     3961 caaggaaatc aaccaggatt tccacggcat ctacgtaccg aaagagccgc aactgagcgc
     4021 caagggcatt gccgttgcgc tggccgacgg catcagcagc agcgcggtca gcgacgtggc
     4081 cagccagtac gccgtcacgg gtttcctcga agactattac tgcacctcgg aggcctggtc
     4141 cgtcaagacg tcggccgagc ggatcctgag cgccactaac tcctggctgc atgcgcagac
     4201 gcagaaaagc cagtaccgct acgacaagga caagggctat gtgtgcacgc tgagtgccat
     4261 ggtcatcaag tccaccaccg cgcatatttt ccatgtgggg gattcacgca tttaccgcct
     4321 gcgcggcagg gcgctggagc aactgaccag cgaccaccgc gtgtggatct cgcagggcca
     4381 gagctatctg ggccgcgcgc ttggcatcaa accgcagctg gaaatcgact accagaccca
     4441 gcaggttgag cagggcgaca tttttctgct ggccacagac ggcgtgtacg aacatgtcag
     4501 cgagcacgac atggctgcca tcatccacat ctgtgccggt gaacccgacg ggctggagac
     4561 ggccgccaga tccctcgtcg agaaagcctt ccagcagggc agcaccgaca acctgacggc
     4621 ccagatcgtg cgggtcgatg ccttgccccg ccagcacgcc ggcgaggtgc tccagcagct
     4681 gtccagcctg ccgctaccgc ccatcctgga tgcacggacg gcatttgacg gctacaccat
     4741 catccgcgaa gtgcatggca gcagccgcag ccacatctac ctggcggtgg acggtgaaac
     4801 aggcgcccag gtcatcctga aaacgccgtc gatcgacctg caggccgacc cggcctacct
     4861 ggaaaggttt ctgaccgaag agtggattgc ccggcgcatc aacaatgcac atgtgctcaa
     4921 gccctatgta ccgacccgaa agcgccacta cgtttacgtg gccaccgaat acattgacgg
     4981 gcagaccctg agccagtgga tgatcgacaa ccccaggccc gagctggaag ccgtgcgcgg
     5041 cgtggtcgag cagattgcca aagggctgcg cgcctttcac cggctggaga tgctgcacca
     5101 ggacctgcgg ccggacaaca tcatgatcga ccggaccggc acggtgaaaa tcatcgattt
     5161 cgggtccacc cgcgtggcca gcctcacgga ggcggctgca cccggcgagc cggaccccgt
     5221 gctgggcacg gcccagtacg ccgcacccga atattttctg ggcgaaggtg gcaccacacg
     5281 ttcggacatc ttctcactgg gcgtcatcac ctaccagatg ctcaccgggc gactgcctta
     5341 cggcgccgaa gtggccaaat gcagaacccg ggccgcacaa aacaggctgc gctacgactc
     5401 cgcgctgcac gacgaccgcg agatacccgc ctggatagac ggcgtgctgc gcaaggccct
     5461 gcatccggac cctgcccggc gttacgacga gttgtcggag tttttatacg acctgcggca
     5521 ccccaacagg gcgtttctgg acaagacgcg cccgcccttg ctggagcgca atcccctgct
     5581 gttctggaag ggcgtctccc tgattctggc agggatcatc gtggtgctgg tgggcgtcca
     5641 ggcattcgtt cgttaggccg attccccacc agcggcagag cgctgcctgt gggcatgcgt
     5701 cctggcaccc ggcctgtcag ccgtgttttc ccttctccca gcccaccgcc ccgatcgccc
     5761 gctcggccac ttcggcggcc aggggctcga ggctggtggc catcgtgtcg ttccagcggt
     5821 tcaggtagcc gaacagggcg atggccgcga cgatctcgac gatctgctcg tcgttgtagt
     5881 gtttcttcag ttcgtcgaag tcttccgcag aagcttcgtt gggcacagca ccggccttga
     5941 gcgccaggcg aatggccgcg cgttctgccg ggctgaacaa gtcgctgttc tcgaagtccc
     6001 acagcgcggc gatcttggcg tccgaggcct tgtagatgct ggaaaggttg gtcatgtgcg
     6061 actggcagta caggcagccg gccgcatagc tgctggccag gctgaccagc atcttggttt
     6121 cttccggtac cgtgccttcg tacaacacgg cctggttcag cgccatgaag gcccgcacga
     6181 tgttgggccg ccgcaccatg gtcatgatgc tgttgggtgt gaagccgcgc gtttttttgt
     6241 agtgctcgaa acgctcgcgg atgtcgtctt ccttgatgtc tgcgaggggg acggggttca
     6301 ggtgtgccat gcgggagtcc ttgttgttct gtcaaagcgc catgttagcc cgcctcaccg
     6361 cgcagattcc cccgctacac tttgcccgtc ttcacctcgg gggaatccca tgagccgttc
     6421 ttgcatgtgg tgcttcagtc tgctgttggg cgtcacgtcg ctggcctggg cgcagtcacc
     6481 taccgagtgg cgcgggtcgt ctccggcaga gcaggtgctc gatgccgcag cgttcaaagg
     6541 ggtgggcgaa accatttccg aacatatggg cgacgtgcag agcgtggtgg tggtgttgcg
     6601 cggtcggaca gtgttcgagt tctaccgcga tggtgccccg gccatcctgc gcgatgtgca
     6661 ttccgtcgcc aaaagcgcct tgtcggcact ggtgggcatt gccgtggggc agggccgcat
     6721 tgccggcctg gatcagcccg tgctggcgct gatgcctgaa tggacggcat tgaaccccga
     6781 tccgcgcgcg gcgaccatca cggtgcgcca tctgctcacc atgagcgcgg ggtttgaggt
     6841 gaatgacccc accggaacag ccgccgcagg ccggccgcag gacacctggg cgcggccgct
     6901 gcgcagcgcg ccgggcgcgg catttgcgta tgacaacgcc ttgattccca tgctgtcggc
     6961 cgtgctcgaa cgggccaccg gcatgcctct ggcggactat gcgcgcgagc aactggtgac
     7021 acccctggtg tttgcagagc ccgcctacag ccgcgggctg ggcctgcgca cgctggacat
     7081 ggccaaactc ggccagcttt ttttgcagaa cggcgtgtgg ggcggaaaac aaatcgttcc
     7141 acaggcctat gtcatggcgg caacccaccc gcaaaatgcc ggcggcccgc ctgtctcctt
     7201 gtcttatggc tacatgtggt gggtggtgcc gtccagcgcg gcgcgtcaaa ccttcatggc
     7261 cagcggcttt ggtggtcagt tcatctgggt gtatccgccg c
//
LOCUS       DAIDFG010000159         7252 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_159_length_7252_cov_17.518133, whole genome shotgun sequence.
ACCESSION   DAIDFG010000159 DAIDFG010000000
VERSION     DAIDFG010000159.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7252)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7252)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7252
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159_length_7252_cov_17.518133"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1284 a         2521 c         2327 g         1120 t
ORIGIN      
        1 ccgagggggc gctgcgcctg cggcccggca aagccggttc cgcggccccg gctggcaact
       61 cgggcgattg gcaaccaggg actacgggcg ggcggtatcc tcggccccgc gcaccaccag
      121 caccggaacc ggcgcggtcc gcacgatctg ttcggcgtcg ctgcccatca ggacacggcc
      181 catgccgcgc cgcccgtgcg aaccgaccac aatcaggtcc gcccccgcgc tcctggcctg
      241 ctcggccaca tgttcgcaga tgcggcccgg cccgcccaca atcagcacac tgtcggtgtc
      301 cacgccctgg tccacggcct tttgccggcc gagcgccagc agtttctcac ccgccgcctg
      361 catcagggga acgatttcgt tggtgtaatt catcgccgtc tcgaagccgt tgacgtaact
      421 cagctcgtcc accacatgaa gcagccgcag ccgcgcgccg gtcagccggg ccaggcggat
      481 ggcttcgtcc agcgcctttt ccgaagtcgg gctgccgtcg aagggaacaa gaatctgatg
      541 gtaagcggac aatttttctc ctcagtcaaa tcattttcag cgcagcgaca ggcgcatcgc
      601 cacggtttcc tgcgcgcttg cggtggacac gtcgaacctg gtctggcgcg ccagggcggc
      661 catgcccacg ttctccggca ggcactgccc cacaacctcg gtggtgccgc gctcgcgcag
      721 gtagcgaatg agcttgtcca tcagctggcg ccccaggccg cgcccctgcc agtccgtggc
      781 cacctgtatg gcaaattccg cctgcaggtt gtcggggtcg caaacggccc gcgcttcgcc
      841 caccatgacc ggctcgccgc ccgcctgcgg caccatcgcc acaaaggtca tctcgcggtc
      901 atagtcgatc tggctgtaac gggccagctc ggaatgcggc acctcgcgcc gcgtcatgaa
      961 gaaacgcaag cgcatgtcgg caggtgacgc cccggcgtaa aaagccatca ggcgctggcc
     1021 atcctcgggg cgaatcggac gcagcagcac gtcctggccc ttgacctgca catgctcctc
     1081 cagggtctgc gggtaaggac ggatggccag ccggctgcgc tcgcccgcgg gaaccgggcg
     1141 caaccggacc cgcgcatcga gagccagcac accgtgttcg tcaaccagca gggggttgat
     1201 gtcgagttcg gccagccagg ccaggtcgca agccatctgc gagaccttca gcagcgtggc
     1261 cagcaacgcc gcctcatcgg cgggcgggcg cccgcggtag cccgccaaca gcagcgatag
     1321 cctgctgccg gcgagcatgt cctgcgccag ccgggtgttg agcggcggca acgccaccac
     1381 atggttcttg tgcagctcga cggccacgcc accctcgccg agcagcatca ccggcccgaa
     1441 cacggggtcc gaggcaatgc cgacaatcag ttcgtgcgcc ccggggcgct cagccatcgc
     1501 ctgcacagtg aagccgagta tctgcgcctg cggcagcagc ccggtcacct gccggcgcat
     1561 tttctcggcc gcgcgcccga gttcttcggc tgaagccagg ttgagcgcca cgccgccgac
     1621 gtcggacttg tggatgacct ggggcgacac gatcttcagg gccacggggt aaccaatgcg
     1681 gttggcggta gcgaccgcct cctcgacgtc gcgggtcctg acggtttcca ccgtggggat
     1741 gccgtaggcc cgcagcagcg cctgggcctg cgcatcaccg agccactcgc ggccttcctg
     1801 cagcgcctcc tcgagcagcg actgggccag ttgccggtcc ggcgtgaagt cctccagtgc
     1861 ggacgcggga agctgctgca gcgcctgctg gttgcgcgcg taggtggcca gttgcagcca
     1921 cgccgctgcg gcgcgctccg gcgtgttgta gctggcaatg ccggccccgg cgctggcccg
     1981 ccgcgccgcc gccaccgccg caccgcccag ccagcaggtc agcaccggtt ttgcggacgc
     2041 ctggatcaag ggcaggcagg ccgctgcaat ctggtcggca ggaacaatgg cggtgggggc
     2101 atggatgaac agcacaccgt cgacctcggg tgccgccagc aagacccgca ggctgtccgc
     2161 ataacgactg acgggtgcat caccaacaat gtcaatcggg ttgccatgcg accaggtagg
     2221 cggcaggcac tggttcagcg cttccagcgt cccttcaccc agcaccgcca gttggccacc
     2281 gcccagcgct agcgcgtcgg cggccagcac ccccgcaccg ccgccgttgg tcaggaccgc
     2341 cagccgctcg ccgtgccaac ccctcacatg ggtcagggtt tcggcggcgt cgaacagcgc
     2401 ctccagcgtg tccacccgca acatgccagc gcgccggacg gcggcgtcga acaccacatc
     2461 ggagccggcc agcgcaccgg tgtgcgaggc cgcggcccgg gcgccgtcag gcgcacgccc
     2521 ggccttgaca acaatcaccg gcttgttgcg cgcggcggcg cgcgccgccg acatgaactt
     2581 gcgagcggtc ttgaccgatt ccatgtacat caggatggcc cgcgttcctg gatcgctggc
     2641 caggtagtcg atgacgtcgc cgaagtccac gtcggcgctg tcgcccagcg agatgaaatg
     2701 cgaaaacccg atgccgcggc tgttggccca atccagcatg gcggtcgcca gcgcgcccga
     2761 ttgcgtaacg aaagccagct tgccgggcaa ggcgttgccg ggtgcaaaac tggcattgag
     2821 cccgatcccc ggcaccagcg ccccgatgca gttgggcccg aggatgcgca gcagccaggg
     2881 ccgcgccgca tccagcatgg cctgctccag cgtgggcccg cccgcgcgcg aggcctgctt
     2941 gagcccggcc gacagcacga tggcggcgcg cgtgcctttg cggcccaggg cggtgatcag
     3001 gtcgggcacg gtcgcggccg gcgtgcagat gatggccaga tccggcgcct gcggcagcga
     3061 ctcgacatcg ggccaggccg ggccaccacc cacctgggaa tgccgacggt tgaccggcca
     3121 gaccgggccc tgaaacccgt cagccctgag attgcgcagg accacagccc ccagactgcc
     3181 ctcgcggtcg gaggcaccta tcagggccac agaggcgggg cggaacaggg tctcgagatt
     3241 gcgtacgctc atgaatttcc ttggaacaag agtcaaacat accccatgct ggaagattag
     3301 cgcccggtca gcaccctcag ttgctccaga tcaaagaccc gggtgccggg tggcccacag
     3361 aatggtcgcg ccagcacaag gttctccatg gaaacatacg attgcaccct gatgccgcgc
     3421 ttcagccaga ttctggcgca gcgcgaagcc gaactgcgtg ccctcctgcg cgccaccgcc
     3481 gagcaccccc aggaagcggg cagcaccgga caacaccggg tgatggattt caaggatgtc
     3541 gccatggaac aggccctggc cacggtcgat gaggccaagg ccggacacgc ggtgcatgaa
     3601 ctcgaggagg tgctggcggc gcggcgccgc ctggccgatc agcgcttcgg ccattgtctg
     3661 gactgtggcg aagcgatcga tctgcgacgc ctcgcggccc ggcccgccgc ggccctgtgc
     3721 accacgtgcc aggccattca tgagcatgag cgtcccatgg cgatgcggcg ctgaaccggc
     3781 aaccatcacc ccacccgaaa ggaattccca tgcgtgcaaa aacgtccctc cccgtcccgt
     3841 cccgcgaaag cacgggtatg ggcgccaccc ccgccagttt tctggccgac cttggccgcc
     3901 agcaactggc cgtcgccgcc gatgcggcgt gtgccctgtt gcgcggcagc gaagccatgc
     3961 gggccatcca gcatcaggcg gcccacgagg cttccctgcg tcaccaggcg gcggcgcaaa
     4021 agctgcatgg aaactgtgag ccggccgacc tgatggccat ccagtcggat ctgctgagtt
     4081 ttgacctgca gggtgccacc cagtactggc aacagatcac ggccgcggca ctgcagtcgc
     4141 aggtcgagat gatggccagc gccacccacc tgctcgacag cgaacagggc gacgcattga
     4201 aatcggccat ggaaagcttt caggcggcga tcgcatcggg caatggcttt tttgcgggga
     4261 aacccgccgc cacggcgcca ccaacccgcg tctgaagcag gttacccgcc gaccgtgtcc
     4321 gcgggcaggg gtactgaaac cgcaccttac ccttggtgaa cgtaagtacc gggtgcggct
     4381 gacagccggc ccgcagaacg caggccgacg accggcgttg ccttggtttg tcccgtcgat
     4441 tgttcatgca gccaggcgtg ccagcacggc caccaggagc cggtgaccgg cggcgcacct
     4501 tgcagccagg cctgcggatc cgcaggcgcc ttgccttccg catgggtggc gacgcggtaa
     4561 ctggcctggg catgggctgc ggcgccggac ggcggattga cgatgccgac gttgtgccct
     4621 cccgaggtca gcacaaaact cacaggcgcg cgtgtcaggc ggtgaatctt gtagaccgaa
     4681 gtccacggcg acacatggtc gcgctcggtg gccaccacga acagcggcac cttgatgtcg
     4741 gccagcgaga tggcgcggcc acccgccaca tagcggccct cggccaggtc gttgtgcagg
     4801 tacagcctgc gcaggtactc gctgtgcatg cgcgccggca tgcgcgtggc gtcggcattc
     4861 caggcgcgca gcgccgtcat cggtgtctgc gcgcccatca ggtattcatg cacgagtttg
     4921 gaccacacca gatccttgga attgatcagg gcaaaggcgc cggccatctg gcggccgtcg
     4981 aggtagccac gtcccgccat caggtcttcc agaaacgcaa tctggctctc gtcgatgaac
     5041 agccccaact cgccaggctc gtgaaaatca acctgcgcgg ccagcaatga cacggttttc
     5101 agcgggttgc gcggccgggc gcccagggcc gcagccgcca tggccagcaa ggtgccgccc
     5161 aggcaataac ccgccgcatg aatgccggcc ccgggccgca actcgcccac cgccttcagc
     5221 gcggccatgg ccccgagttc caggtaatcg tcgagcccta ggtcccggtc ctggctgtcc
     5281 ggatttttcc aggaaaccat gaagacgctg tggccctgat ccaccaggta gcggaccagc
     5341 gaatcggccg gcgtcagatc aaggatgtag tacttcatga tccacgaggg cacgatcagc
     5401 accggctcgg catcgacctt ggccgtggcc ggctcgtact ggatcagttc gatcagccgg
     5461 ttgcggaaaa ccaccttgcc cggggtcagc gccacccctt ttcccggaag aaaggcctcc
     5521 acgccgcgtg gcttgccctc cgtcagaacc gccatggcgt cgcgcgacca gttggcaaaa
     5581 ccgctggcca gattggcccc gcccgtggcc agggtttcct tcagcacctg ggggttggac
     5641 atcggaaagt tcgagggcga ccacatgtcc agccactggc gggtggtgaa tgccgccacg
     5701 tcttcgtggt gccgggagac gccgcgcaca cctgtcatgg ccccgttcca ccactgctcc
     5761 tgcagcagga aggcctgagc catggcgctg aacggcagca tgttccactc ctgcggcgag
     5821 aagcgtttgt cctgggggac aggctccacg cagcgcacac agtcgccccg ccaggcctgc
     5881 ggcgcatact gcgcccacag cagacccttg cgcagcgcgt cggcaaccaa ttcggcctgc
     5941 cgggaaggcg acacgagcag gtgcgtgaac cagtcggcat acgccccagc gacagaggca
     6001 ggtgagacgc cgtgcgtgag ccgggccagc ccggccttga gtggaaggtc catcggagcc
     6061 ggcgtgtgcc cgtgcgggcg ggagtcagaa agtgccatga aaaagtccgg cgccactatg
     6121 ccgcgcctcc agtcagtcaa aagcatgatc gtcccgtagc agcgccaccc cgcatttgcg
     6181 caaggtcaac gaaacccctg aatcccggtc cacaacctcg ccagctgcgt accatgcccg
     6241 caagtcgccc gaggccatgc tcgcgatgac cgtcgacccc tccagtgcga aattgcagcc
     6301 tcatcgaaac ctgtgccgca ggcgccgcgt ccctgcgtga gccgggttcc gcgccgactt
     6361 gcgccatgtc aatgcatgcc gcgcaaagag gaatagattg agcgtcttgg cagtgccata
     6421 ccgccgcatt cagattccaa cagccatcag gagagttcca tgaaaacgga tgcccacatc
     6481 aaggccgacg tcgttgacga gctcgcgtgg gatcccgccg tcaatgccac cggcattggc
     6541 gtggcggtca gggagggtgt ggtgaccctg accggccacc tcgacagcta tgccgaaaag
     6601 catgcggtgg agcgggctgt gcaccgtgtg gccggcgtgc gcggcatcgc ggtggagctt
     6661 gatgtcaggc tggccgccga acacaaacgc agcgattccg acatcgcgca ggcagcggcc
     6721 acagccctgc agctgaactc cctggtgccg gacgagaaga tccaggtcct ggtcgaaaac
     6781 ggccgggtca ccctcaccgg cgaagtcgac tggagctacc agctggccag cgccgaacag
     6841 tgtgtgcggc cgctggccgg cgtgcgcggc ctgtccaacc gcatcaccat caagtcccgc
     6901 gcaagcagca aggacgtcgg cgcccagatc accgcggccc tgacgcggca ggccgcgcgg
     6961 gaggccaaac acatcaccgt ggaggttgaa ggcagcgtcg tcaccctctg ggggaaagtg
     7021 cattcactgg cggagcgcga agcggccgta ggtgctgcgt tctcggcgcg cggtgtttcg
     7081 cgcgttgtgg acaaactcga ggtcggtgcc tgacctggaa ggtacggttt ttcattattt
     7141 ttttaatcat tcaaggagtg tcaatcatga gcaatcttcg tgtcctggac cccaccttca
     7201 ccgattcctt cgagtcggcc atgcgccgct tcttctcgcc cacagttttc ga
//
LOCUS       DAIDFG010000160         7201 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_160_length_7221_cov_19.346916, whole genome shotgun sequence.
ACCESSION   DAIDFG010000160 DAIDFG010000000
VERSION     DAIDFG010000160.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7201)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7201)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7201
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160_length_7221_cov_19.346916"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1254 a         2248 c         2294 g         1405 t
ORIGIN      
        1 gtgtttcgaa ggccggcccc tacaaggcaa atacgtacag gtattgatgg tctcggcgaa
       61 ctgcatgcgc gttcgccttc tggcatttca ccgttcgggc tgagcctgtc gaagcccttc
      121 gacaggctca gggcgaacgg tttgtgcttt ttgatgaacg gtgctcaccg gacgccgaac
      181 cacctgttgg aaaaccatgc gcgctgacct cttcctcgtc gacaacggct ttgccgccac
      241 ccgttcccag gcccagcgcc tgatcgcttc gggcgtgttg tggcgcgccg aggaaagtgc
      301 gccctggaag aaagtcgcca aaaacggcga cgaaattcct gaaggcgccg agctgcagat
      361 tcccgacacc acggaagcca agtacatctc gcgcggcggg ctcaagcttg aaggtgcgct
      421 caaaagcacc ggcctgagcg tggccggtct gcgctgcctg gacatcgggc aatccaccgg
      481 tggcttcacc gactgcctgc tgcagcacgg tgctgtggag gtggtgggcg tggatgtggg
      541 ccatggccag ctgcacccca gcctgcgcgg cgacccgcgt gtggtgtgca tcgaaggcct
      601 taacgcgcgg tccatgactg cttctgattt gatagcgggt cccgtccata cggcggaggc
      661 tgaaggtcaa tttgacgatg gtgatgactt cgaggtggac gaaggcgagg ccgtcgaagc
      721 cgactttgat ttcctgaccg gcgacctgtc ctttatttcc ctgacgctgg tgttgcccgc
      781 cgtggtgcgc ctgctgaaag ccggcggtca tctgctgatg ctggtcaaac cccagttcga
      841 gttgcagccc ggccagatcg gcaagggtgg cattgtgcgt gatgccgtgc atttcgaatt
      901 tgtcgagaaa cgcctgcgtg atgcctgcgc tgcgttgggg ctggacgtga aagcctggct
      961 ggcctcgccg attgaaggcg gcgacggcaa tcgcgagttt ttcattcacg cggtcaaagg
     1021 ccaaagcaca gtcggcgaag accagccgct accggcggcg ccgccgcgca aggccgccaa
     1081 acgcacgccg cgcagcgagc tacgcgcgat gcatgaggcg cacgaggact ctgaagaagc
     1141 ccacgcgggc cagcacggcc cggcgcgcgg caaacgccgc gacaaagacg actgatccgc
     1201 actgcgcagc cctgaactac aagtgagact ggtgatgaat cttccaatca gctttgaatt
     1261 ttttcccccc aagacaccgg aaggcgccga caagctgcgc ctgacgcgcc gggcgctgta
     1321 cgcgcaccag cccgagttct gctccgttac ctttggggct ggcggctcca cccaggaagg
     1381 cactttcggc accgtcggtg aaatcctgaa agagggcgtt gacgctgcct gccatttctc
     1441 atgcgtgggc gcgaccaagg ccacggtgcg ccagcagctc gctgccttgc aggccatggg
     1501 cgtcaagcgc ctggtggccc tgcgcggtga tctgcccagc ggttacggcg ccggcggcga
     1561 gttccattac gccagcgacc tggtggcttt catccgtgag ttcacgggca atgcctttca
     1621 catcgaagtg gcggcctacc ccgagatcca cccgcaggcc aaatcggccg atgccgacct
     1681 gcaggccttt gctgccaagg tcaaggccgg cgccaattca gccatcaccc agtatttttt
     1741 caacgcggat gcctacttcc gctttgtgga ggacacccgc cggctcggcg tcgatgtgcc
     1801 cgtggtgccc ggcatcatgc cgatcaccag ctccacccag ctgatgcggt tttccgatgc
     1861 ctgcggcgcc gagattccac gctggatccg cctgcgcctg cagggtttcg gcgacgacac
     1921 ggcatcgatc aggagcttcg gcctcgacgt gatcaccgac ctttgcgacc agctgcagac
     1981 cgcgggtgcg cccggcattc atttttatac gatgaaccag gccgccgcga cgaccgagat
     2041 catccgacga ttgcagccgg tttgaaggga tccctgttct gaccggcatg ctgaagccgc
     2101 gcgccatcac acaggccatg gccctcggcc tgctggccct gctggcagcc tgtacgacaa
     2161 ccgcgccagc gccacccggc cgctctgcac cccgccctgt gccgacagtc aaaccggcgc
     2221 ccatgcccgc cagccccgag gctgtgggca cacccgcgga cgcggcgccc ctgtccatcg
     2281 ggcccttcag cgatctgcca cgaccggcgt tgctggcggg tgaacctgcg cgcgagttcg
     2341 agcgtggcgc tgcgtcgtgg tacggcccgg gttttcatgg ccggctgacg gccagcggcg
     2401 aacgttatga catgaacgct ttcacggccg cgcaccgcac cttgccgttc ggtaccatgg
     2461 tgcgggttca cagcctggtc aatggccgtg acgtggatct gcgcatcacc gatcgcggcc
     2521 ccttcagccg caaccgcatc atcgacgtga gccgcgctgc cgcggcagaa ctcggcatgc
     2581 tgggcctggg cttcaaggag gtggtgttgc tggtcccgga gtccacaccg gtcatggcca
     2641 ccgcaccgcc gccaccggtc agaaaatccc ggcgcggcgc caggcgcgcg ccagtgccca
     2701 gggtgcgcta gatattttga tgtgaaattg gcctcaagcc cttatttcac gttcggtagc
     2761 agctcctgaa tagatagcaa attaaggtca aaacctaaac gcttgcgcga aagctgtagc
     2821 catggcgctc gaaatccagc gaatcgtaga acgcatgggc cttttcgcgt ttcaggctgg
     2881 aggacagcgt gagcttgtag caaccgcttt cggcggccag cgccatcgca tggcgcatca
     2941 tggccttgcc gatgccctgg ccctgccagg ccgggtccac cgccacgtct tcaaccaccg
     3001 ccgagggcgc cccgaggtgg ccgaggttgt ccatgatgag cagggcaaag gtaccgacca
     3061 cctgcgcgcc cttgacggcg acatacagct tgtagttggg ataactcgcc atgcgggccc
     3121 agatgcgctt tgcatccgcc agtgcaagga tgtctccgtc gtcaatgtca ggctgggcgt
     3181 agaggcgcag tacgtccagc agatccggct cgagaaccgg gcgaagggta agggcatcaa
     3241 gcatgacgcc actgttcccg ggcatcgtgt cgaagagatg acagtccttg aatgaacgac
     3301 gttcacgtag cgcggccggc ttccggacat ctggtcgggt acggagccaa tcggaccggg
     3361 acgtacgcaa cgcaggcggc gtgggcgttt cccctgaaaa tggcgccttt gaaacactct
     3421 gactggagcc gcgtatgaag acgggcgagc tgatgttgtt catcttcctg gcggggctgt
     3481 cgtcagcagc cgtcgcgcag gatcgaaaga atcatttcaa cgatcctttc ctgcaggtga
     3541 cctctgccat ggccggctgc cccgtgcccg aaggcccgat gttcacgccg gaccaggtgc
     3601 gcgcagaggc gcatgtgcgc gcccagcacg gcggcagctg cttccgctcg ggccgttgcc
     3661 gcctgcccaa ctcctatctc tacgacaggg agatcattcc ccgcatggtc agctacctgc
     3721 agcaggacgg gcgcttcaac aacaccagtg tctgggtgct cggcgagcgg cgcctggtca
     3781 cactgatggg ctgcgtgcag acgcaggagc aggccagggc catggagcag gccgtcatgc
     3841 tggtcgatga cgtcatgggc gtggtcaact atctgagcgt gggcgccacc gccaagccga
     3901 aatacagcgt cgtgccggtg cagcccaagc cggccaggtg agcgccggcc tcagttgttg
     3961 gtatcccgca cccggcagca atgtctggca tcgcggattt tctttgctat gttattaata
     4021 gctacctggt tccactgcac aagggttgat ggcatatttt tgctttggga tatcgccctt
     4081 ttcataacct gcgcagaggg atgcccgccg tgccaatatc cgggtgccag cagaaaaatt
     4141 tttaacttac tagaatttca gtaattcctg aaaattcagt aaatccggac tgccatgaac
     4201 ctcgcccccc tgacccagcg ttttgtcctt cacttcgggg aaatgggcag ccgctggggc
     4261 atcaaccgca ccgtggggca gatttacgcg ctgctgtatg tgtcgccgcg cgcgctcaat
     4321 gccgacgaga ttggcgaggc gctggcgttt tcgcgctcca acgtcagcat ggggctcaag
     4381 gagctgcagt cctggaacct ggtgcggctg cagcacctgc ccaacgacag gcgcgagtat
     4441 ttccaggcgc ctgatgacgt ttgggcgatt ttccgcaccc tggccgagga gcggcgcaag
     4501 cgcgagatcg accccacgct gtccatgctg cgcgacgcgc tgatggagca gccttcggcg
     4561 cctgacgaca tccacgccca ggcgcgcatg aaacagatgc acgacctgat cgagctcatg
     4621 accggctggc tggccgacgt gcagaaaatg gactcggcca cgctggtcag cctgatgaag
     4681 atgggcgcca aggtgcagaa gctgctcgag gtcaaggaca aggtcagcag caaggtgaag
     4741 gccggcttga caggccgggc cgctgcgccc aagctggcgg cactgccggc ggacgctatc
     4801 cccgaagaaa ccaacaggag ttgatgatgg acggattcga cgcattcctg ctggcgcgca
     4861 ttcagtttgc ggccaacatc acttttcaca tcctgtttcc gacgatcagc atttcgctgg
     4921 cctgggtgct gctgtttttc aagctgcgct tcgtcaagac cggcgagcgg cactggatgg
     4981 atgcctacca gttctgggtg aaaatttttg cgctgacctt tgcgctgggt gtggtcagcg
     5041 gcatcaccat gagtttccag ttcggcacca actggccggg ctacatggaa aaggtcggca
     5101 acatcgccgg gccgctgctc gcctatgaag tgctgacggc tttcttcctg gaggcgacca
     5161 tgctgggcgt gatgctgttc ggccgcgaac gtgtgagcga gaccctgcac accgtggcca
     5221 cgctgctggt ggccggcggg accacactct cggcgttctg gatcctggcg ctcaactcct
     5281 ggatgcagac gccggccggc tttgaaatga tcaacggcca ggcccatgtg acaagctggt
     5341 tcgaggtgat cttcaacccc tcgtttccct accgcatggt gcacatgctg ctggcttccg
     5401 gcctgacggt ggcgtttctg gtggccggca tctcggctta ccgctggctg cgtaatgacc
     5461 gcggcgccga ggtcatggcc agcctgaaga ccggggtgta catcgcggcg ctactgattc
     5521 cgctgcagat catcgcgggc gacctgcacg ggctgaacac gctggaacac cagccggcca
     5581 agctggccgc catggaaggc atatggaaaa cggaaaaagg cgttcccgcc gtgctgtttg
     5641 gcttgcccga cgagaaggcg aaggagaatc gctacgaaat cgccatcccg aagctggcgt
     5701 ccctgtacct gacgcattcg ctcgatggcg aggtcaaggg cctggaccag tttgaaggca
     5761 aacacccgcc ggtggcgccg gtgttctggg ctttccgcat catggtcggc gtgggtgtgc
     5821 tgatgctgct ggtcagctgg gccgccacct ggcaacttgc accgtggaag aaggggacgg
     5881 aacgcaagct gcggccctgg catgcgaagc tgctggtggc catgactttt gccggctggg
     5941 tggcgctgat cgccggctgg tatgtcaccg agatcgggcg ccagccctgg ctggtgaccg
     6001 gtgtgctgac ggcggccgag gcggcgtcca cggtgccggc gccgcgcatt gcactcacac
     6061 tgtccctcta cctcacgctg tatgcagcgc tgatcgtggc ctacatctcg gtggtgtttt
     6121 acctggcgcg ccacagaaaa catgcggaca agccgtttgg caccggcctc gaagacacgc
     6181 ccaatccggt gacctacccg caacaggaag gagctcccca tgcttgacct tgcgcctgac
     6241 ctgacgttag ccgcgggctg gatgcccatt gttttcctgg tggtgatggg gctggcgatg
     6301 ctggcctacg tgatcctgga cggttacgac ctgggcgtgg gcgtgttgat gcgccgcgcc
     6361 agcgacgacg acaaggacac catgattgcc agcatcggcc ccttctggga cgccaatgaa
     6421 acctggctgg tgctgggcgt cggcatcctg ctcaccgtgt tcccgctggc gcatggcgtg
     6481 atcctggggg cgctgtatct gccggtggcc ttcatgctga tcgggctgac gctgcgcggc
     6541 gtggcctttg acttccgcgt gaaggcgcgc gccgcgtaca agccgctgtg gaaccgcctg
     6601 ttctacaccg gctcgttgct ggccagctgg agccagggtt acatgctggg ctcgctgatc
     6661 acgggttttg ccgacacatg gtggaccatg gcgttcaacg ccgtgatcgg gttggcgctg
     6721 gtggccgcct actgcctgct gggcgccggc tggctcatca tcaagtccga aggcgcgctg
     6781 caactgcagg cggtgcgctg ggcgcgcggc agcctgctgt tcaccctggc cggtgttgct
     6841 gccatctcga tcgccacgcc gctggtcagc cagcgcattt tcgaaaaatg gttttccttt
     6901 cccaacatcg ttttgttgct gccgattccg gtggccaccg ccgtgttgtt tgccattatt
     6961 tttcgcagcc tgcagcgctt gccggtgcga ctgggccagg gcaatgaata cggcgcctgg
     7021 gtgcccttcg gtgcgacggt gggcgtgttc ctgctggcgt tttacggcct ggcctacagc
     7081 ctgtttccgt ggctggtcat cgaccggatc acgatctggc aggcggccac ggcgcccgaa
     7141 gcgatgggtg tgattctggt gggcgcgctg gtcgtgctgc ccatgatcgt cggctacacg
     7201 g
//
LOCUS       DAIDFG010000161         7219 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_161_length_7219_cov_17.357761, whole genome shotgun sequence.
ACCESSION   DAIDFG010000161 DAIDFG010000000
VERSION     DAIDFG010000161.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7219)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7219)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7219
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161_length_7219_cov_17.357761"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1211 a         2184 c         2370 g         1454 t
ORIGIN      
        1 cggctctgcc ggcccgcagg cgcagcgccc cctcgggggg cagaaagcta cgcgaagcga
       61 gcgaacgtgg gggcgttatt tgtacccgac gcggtccagc atctgctgca ccttcacctg
      121 gttggcgccg acggtgctga tcgggatggt ctcggacttg aagctgccgc ccgacatggc
      181 ctgcagggcc gcattgctga ctttcacgcc ggcgacggcg ggccactcgt tgttgccgtt
      241 ggcaaagtag ttctgcgcat cggggctggc taggtactcc atgaatttca ccgcgttgcc
      301 gatgttcttg gcgtttttgg cgacggccgc accggcgatg ttcacatggg tgccccagga
      361 ctgctggttg ggaaacacca caccgacctt gtcggcgatg gccttgtctt ccggcgcgga
      421 tgaccgcagg atgcgcgcga ggtagtagct gttggtgatc gcgatgccgc attcgccgct
      481 ggccacgccc ttgatctggt cggtgtcgcc gcccttgggc ggacgggcca tgttggccac
      541 catgcccttg agccattctt cggccctggc ggcgccaaga tgctcggtca ccgcgccgaa
      601 cagcgacaga ttgtaggggt gcgagcccga gcgggtgcac agcaggccct tgtttttcgg
      661 gtcggcgagt tcttcgtagg tgtcgacgtc tgctttcttg accttgagct tgtcataaac
      721 gatgagacgg gcgcgggtgg aaaaaccgaa ccaggtcgtg ccttcgggcg tggcctgggc
      781 gcgcagctgc gcgggaatcg ccttttccag caggggcgac ttgatgggct ggaacaggcc
      841 ttcgctgtcg cctttggcca gccgcgctgc atcgaccagc agaatcacgt cggcggggga
      901 ggctgcgcct tcggctttca ggcgcgcgag aatgccggca tcgtccgcgt ccacgcggtt
      961 gatccggatg cccgtggttt tggtgaaatt gctgtaaagc gcctcatcgg tctggtagtg
     1021 gcgggcagaa tacaggttga ccacctgttc ctgcgcgctg acgccgccag cgccgcccat
     1081 cagggtggca gccacggcca gggtggccag aatcgggaat tttttcaaca tggtgcgaac
     1141 tgtgaaggtt ggaatggcaa tcagtgtaat acaaacacga attattctca atagctttgc
     1201 ggtcgtgacg gctgccctgg gttgggcggg cggctcgctg ggtcgcgggg cagcacgtgc
     1261 ggtcgccgtg gccgggctgg tcatactggc cggctgcgcc ggcctgccgg ccggcacggt
     1321 ggcgcccgtt gtcggtccgg tcgtcgagac cccggcccag cgcgcgccgc gtatcggcct
     1381 ggcgctgggc ggcggggcgg cgcgcggttt tgcccatgtg ggcgtgattg cggtgctgga
     1441 agaagccggc atccggccct ccgtggtggt gggtacctcg gccggcagcc tggtcgcagc
     1501 gctgtatgcc agcggcaaaa gcagtgcgca gctgcagcag acggccctga acatggaaga
     1561 ggtcgcgatc accgactgga tgctgccgat tttcggccgc ggcatgtttc gcggcgaggc
     1621 tcttggccgt tacgtgaacg atctggtggc cggccgtctg atggaaaaca tggccattcc
     1681 gctgggcatc gtggcgaccg acctgggcag cggccagccg gtgctgttcc agcgcggtga
     1741 caccggcatg gcggtgcgcg catccagcgc cgtgccggcg gtgtttgtgc cggtcaaaat
     1801 caacggccgc gagtatgtgg acggcggcct ggtctcgccg gtgccggtgc gatttgcgcg
     1861 gcagatgggt gccgagctgg tgattgccgt cgacatctcc aatccgcccg aagccaacac
     1921 ggccgacggc accttgcaga tcctgctgca gacctttgcc atcatgggga aaagcatcaa
     1981 ccagtacgag ttgaaggatg ccgatgtagt ggtgcagccc tcgcaggcag gcctgaaaag
     2041 cgccgacttc tcggcccggc aacgtgccat cgatgcggga cgggcggcga tgcgggcggc
     2101 gctgcccgca ctcagggcga agatggccac cgtgcgcacg gcagcgcgct gacgctctgt
     2161 ttttgatagc tgcgtacgga cgaccagcaa gggctcaagg ccttttttgg ccaaaaaaaa
     2221 gcccgatctt gcgatcaggc tttgaagggg tccgtgaaac ttgatttctg gaaggacccg
     2281 aggagacaac cggctggaac ttgcgctccg atacttgttt aacggcagca ggctccggaa
     2341 tctacaggat ttgagtttct caaacccgac ctgaatcagt tggtggctcg gcccgctaat
     2401 tcaagccctg ttcatcaacg ctttttggaa gcggttttgg tggcgttcac ggcggtgttg
     2461 gtcaccgcgg tgaggttggc ttcggccatg tcgctggctt gtttgacggc tttctggacc
     2521 gactcgaaag cgttggtggc ggcagccacg gcgcttttca tcacggccac agcggactcg
     2581 gaaccggccg gtgcgttttt ggcggcgctg tcaaccaggc ccatgaattt agcctgggct
     2641 tcggcagcct ggccttcgaa ggccttgccg acttcagcac cggtgccggt ggcgatgtcg
     2701 tacaggtggc ggctgtaagc agcggttttt tcagccaggg gctgcatcag gctggcttgc
     2761 agggccagca gttcctgggc gtctttggcg ccgagcaggg tctgggtgtg gttggcggtt
     2821 tcagccaggg cagccttgga agcctggaca ttgagttcaa ccagtttctc gacgccttcg
     2881 aaagccttgc tggtcagacc aaacagggtt tcaacggttg ctttgtggga agccatgatt
     2941 tgttcagcgg tcaacatatt taaatctcca ataagtgggt taagaattca attagttgcg
     3001 ctgccggaca atctgctgtt gggcttgtct tgtcgattgc tgcactgcaa cataactcaa
     3061 gtatagggat caggcgcacg attgcaagcg atttttgttg cagtgcagca ttttagagat
     3121 caagctctag cgcccgccct gttgagaggc gggaggcgca gaggcgcctg ccggagggcc
     3181 aggcgctgtc aaggggaggg gcctggaacc ccgccggact cagtagcgca gctgcccctc
     3241 gttgtcgatg atggccatct ccacataggt ggacccgttg tgtgactgcg gcgagtactt
     3301 cgccacaaaa cggccgaagc tgatttcgcc ggccccctcc atggccttga tgaacgagtc
     3361 cgtgctcagt ggccggccgg ccctgcgcag accctcgacc agcagtttgg cgtggacaaa
     3421 cccctcgaac tgcgaggccg aagggcgcgc gtccggcgat ttttcgcgca ggagcttcag
     3481 gtactcggcc acggccggga tggtcgtgtt gcgcagcgac ggcatgatct gggtcaggat
     3541 gatcccgcgc gcatcctgct tcagctcgcg gttgatcacg tccacactcg cgacggaaaa
     3601 accatagaag ctcggcttga cggaagtctg caggacggcc ttgacgtaat ttgtgaacgt
     3661 ggtccctgcc gtgcccagta tcacggcctg cggctgagcc ttttcggtgg cggtagcggc
     3721 tgccttgaag tcgggttggg ctggatcgat ctttgtttcg acgacgaagc tcattttttc
     3781 gagggttgcg gcctggcgca attcggcgag gagggttttg ccgaggccgt cgtcctggta
     3841 gaacaccgca atgcgcgaaa tgcccatctg cttcaattgg gaaatgatgc gccgcgcttc
     3901 attggcgtag ctggcgcgca catgaaaaag gtaggggttg aacttgtccc tgaaagcgga
     3961 ggcgcccgtc accggcccga aggcgatggt ccgggtttcc tgcagcagcg gcaggatcgc
     4021 gcccgtctgc gcggtaccgg tgctgttgta gatcaagaac acgccgtctt cctcgatcag
     4081 cttgcgggtg ttgtccaccg cgcgcttgac gtcgaagcca tcatccagcg tggtgtagct
     4141 gatcttgcgt ccgtgcacac cgccggccgc attcaccgcg tcgaagtaca ggcgcgaacc
     4201 cacgccgtat tccttggtct gggcccccaa tggaccgctg agcacggccg acgcgccaag
     4261 tcgtatttct gtgggcgtca cgcccttgtc gcctgcaatc gcctgggcgc ctgcaaacac
     4321 catcaatgcc atgagtgttg aacgaaacat gtgtttctcc ctttaaaact tgttatttga
     4381 agttgttgaa gccttcgtga agctcccttg ccagcctgga tcccgttccc gaaccggctt
     4441 gatggcccag acctgatgcc aggccctcgg gcaacaggtc gcggaacatg ccagtgacca
     4501 gccgcagcgt ctcgcccgcg cgggcctccc cctgggcgct tcccagggct ttcatgagtt
     4561 cgcttcgcag gtcccacatg cgctgcgcgt cacctgcgaa ccggatccgg gttttcagcc
     4621 ccaggaatct gtcgtcaccg ctttcatcca gagccgcgag catggcttcg cgcacctgct
     4681 ccatcctggc ctctcgggca gggtttggta gccggcgagc ccgaaggcct tccaggcgga
     4741 ccgccaggct ggatgcgagc caggacattg ttcgttcctc gatgtggttg agaggggggt
     4801 ctcccgtaaa agcaccgccg atgtagttgt tttgtgtaaa tatggttatt gactgtagtt
     4861 attttgtata actagttcaa gtgccaggaa cgccattttt cctaggagtt acccttggcg
     4921 ctccgggact tctctttgtt ttctggctgt catgcaagct ttctatgctg atcactttgt
     4981 cctgcccttg ccggccgggc accgctttcc gatggccaaa taccagcgcc tgcgtgatcg
     5041 cgtggccggc gagttgccgc aggtgcgcat gggcgtggca ccgcgggcca gcgacggcga
     5101 gctggcgctg gtgcacgcac cggcctacat ccaggccgtg gccgacggca cgctggatgc
     5161 cgcggccatg cgcgagatcg gcttcccctg gagcacggag atggtcgagc gttcacgccg
     5221 gtcggtgggt gccacggtgg cggcggcacg tgctgccctg gggctgcagg gtcgtgccga
     5281 aggcgtggcc gccaacatgg ccggcggcac ccaccatgca tctgcaggca agggtggggg
     5341 cttttgtgtg ttcaacgatg cggccgtcgc ggctcgccag atgcagatgg agtgggcacg
     5401 cagccaatac acgccgctgc gggtggtgat catcgacctg gacgtgcacc agggcaacgg
     5461 gacggccagc attttcagaa gcgatccggg tgtatttacg ctgtcgctgc atgggcaaaa
     5521 gaatttcccc tttcgcaagg aggcgagcga cctcgacgtg gacctgccgg acggctgcac
     5581 cgatgcgccc tacctgcagg cgctggaaca cgcgctcgac gagctggaac ggcgcttcga
     5641 gccaggcctg gtgatctatc tggctggcgc cgacccgttc gaaggggacc ggctggggcg
     5701 gctcaagctc agttatgacg gcctggaggc cagggaccga cgcgtgtttg actgggcctg
     5761 gcaacgccgc attccgctgg ccttcgccat ggccggcggt tacggcatca acatcgacga
     5821 gacggtgcag gtgcagatga atacctacag cgtcgcgatc gagtactgga aacgctggaa
     5881 gatggccccc acggtcgctc actgcgtgta gcttccttcc ccccaagggg gccgctgcgc
     5941 ctgcgggctg gcgaagccag acccgcggcc ccgactcgac ggaagaatgc cgctggcctg
     6001 ctggcaaaat agctctccat gacgtccccg acacgccccc aggccgaacc ccgcagcgct
     6061 tacaaggttt ttcgtccgat cggcacgcgc tggtccgaca acgatgtgta cggccatgtc
     6121 aacaatgtcg tgtactacag ctggttcgac accgcggtca acggctacct gatcgagcgc
     6181 ggcgccatcg acatccacgc cggcgccgtg atcggtttgg tgatcgagac acagtgcaat
     6241 tattttgcgc cgctgtcctt tcccgagccg gtgctcgccg gcatccgcgt ggcgcatgtc
     6301 ggctcgtcca gcgtgcgcta cgaggtcggg ctgttccctg gccaggatgg cgctgccctg
     6361 agcgccgcca ggggccattt catccatgtg tacgtggacc gtcacacccg ccggcccgtg
     6421 cccttgccgc cccaacttct gaccaccctg gagaccctga agtgaccgaa cccctcaatg
     6481 ctgtggatgc agccatccac agtcgcatgt ccgtgcgcgc cttcctgcct aaagccgtgc
     6541 cccgcgaaac gctggaacag ctgctggcga tcgccagccg cgcgccctcg ggcaccaaca
     6601 cccagccgtg gaaggtgtat gtgctgcagg gcgacagccg tgacagtctg gtcggcaagg
     6661 tctgcgccgc gcacgacgcg atccgagcca acccggagct ggccgcgcag tacaaggagg
     6721 aatatgacta ctacccggaa aagtgggttt cgccctacat cgaccggcgc cgcgaaaacg
     6781 gctggggcct ttacggcctg ctgggcatag gcaagggtga caaggacaag atgcatgcgc
     6841 agcaccagcg caactaccgg ttttttgacg cacccgtggg cctgatgttc acgctggacc
     6901 gcgtcatggg ccgcggcagc ctggtggact acggcatgtt catgcagaac atcatggtgg
     6961 ctgcgcgcgg ccacggcctg cacacctgtc cgcaggcggc ctggaacggt tttgccaaaa
     7021 tcatcctgcc gcacatcggc gctgggggcg acgaaatgct ggtctgtggc atggcactgg
     7081 gttatgccga cgaatccgcg ctggtcaaca ccttccgcac gccgcgcgtc ggtgtgcagg
     7141 ccttcacgac ctggctggat tgaatggccc ccacggtcgc tcactgcgtg tagctccctg
     7201 ccccccgagg gggccgctg
//
LOCUS       DAIDFG010000162         7147 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_162_length_7147_cov_20.150874, whole genome shotgun sequence.
ACCESSION   DAIDFG010000162 DAIDFG010000000
VERSION     DAIDFG010000162.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7147)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162_length_7147_cov_20.150874"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1379 a         2117 c         2352 g         1299 t
ORIGIN      
        1 ttggcgccga aaaagtcatt gtcggccagg aagtcgtcga tcaggaagga ggtgatgaac
       61 agcggccgca acagggcctg gatgtcttcg gtggcaggtg tgtaaaacgg gtcggcgctg
      121 cagcgcaggt actggttgta gacagggtgc agttcgcgcc ggtgcggcgc gccgtcgtga
      181 aagccggcgg tggccaggcg cgccggcgtg agcacggtgg ccgacgccat ggggtagtag
      241 ccgtagagcc gctcgcccac agccactccg ggatgcagcg actgcaccac actgccaaag
      301 ccccacaccg gaatggtgcc ccagccttct tcacccgttg ggaagaactg ccagtaattc
      361 atggcgtcgc caaaggcggc ataggtgatg ttgttggagg tcagcgcaaa ggcttcgaca
      421 cgcatgcgca cctggccaac ggccagcggg gcgtcttcgg ccgtttccag ccgcgtgctg
      481 gccagatcgt tttttcggac aagaagggtg gtggtgctca tgtcggcaac ttaaacgaaa
      541 aagcccgctg gctcaagacc agcgggccgc tttggcgaca gggacgtcta acttctggcc
      601 gcaggcttgt ccagccacaa caccagtgcc agcgcgatga tcgcgggcag ggcctgggtg
      661 aagaagatct tcggcgcggc cgtggctgca ccaaagacgc cggccacgat cacgcatgcc
      721 aggaagaaga gcttgaacgt gcggttgccc gtgaacagcc cgaagaaaag acccgccgcg
      781 agaaagccgt tgtacagccc ctggttggcg gccagagcct ggctcgcatc ggcaaactcc
      841 cgcgtgagat tgaaggtttt caatccgggc ggcttgttcc agaggaacat ttccagaatc
      901 aggatgtaga catgctcaat cgccaccaga gcaagggcga cgttgggaat caggcgcctg
      961 ttcatcgtcc ggctcagacc agggtgaggg tcacgtcgat gttgccgcgg gtggcgttcg
     1021 agtaggggca cacctggtgg gcggcatcga ccagcgcctg tgcggcggtg cggtccatgc
     1081 cgggaaggct gatgtccagg cggacagcga tgccgtaacc ggcggggatc ttgcccaggt
     1141 ccacgctggc gtcaatgctc aggtcgtccg gcagcgcaat ctttttcatg ccggccacgg
     1201 ccttcatggc gccgatgaag caggcggagt aaccagccgc aaacagctgc tcggggttgg
     1261 tgccggtgcc ggcggtgcca ggcgagctca gcgtgacgtc caggcggcca tcgtcggttt
     1321 tggacttgcc gtcacgcccg ccagtggtgt gggcgtgggc ggtgtagagg actttttcga
     1381 gttgggttgt catggtgatt tccttgaaaa gatgcttcgg gagtgaagcg ggttgaaaac
     1441 gggaaagggt ttacgcgacc agccgctggc gcagggcccg catttgctgg gtcagcgctg
     1501 tcagttcggg aatggaacac tggctggcct ccaggatgca gccgggaatt tttgcggccc
     1561 ggcttttgag cttgcggcct gccgcggtca gtgtgatgtg gacacggcgc tcgtcttcca
     1621 cggcgcgtat gcgggaaatc agccccgaca cttccaggcg cttgagcagg ggggtgaggg
     1681 tgcccgaatc aaggtaaagg cgttccccga gttcggagac catcagcccg tctttttccc
     1741 acagcaccag catggccagg tactgcggat aggtcaaccc gagttcgtcg agcaggggct
     1801 tgtacagctt ggtcatggcc agcgaggtgg aatacagcgc gaagcagagc tggttgtcca
     1861 gcagcagcat ggcgtcggta gaggtggtgg tttttcctga catgggtcaa attattgcac
     1921 acaatttaat tgtgtgcaag ttgatttggg atcgaggtgt tgcaggggct ttgcagcggt
     1981 ttcgtcaatg ccgagcgcgg gctactaaac tgtgggacca tgcaacaccc ccgttccccc
     2041 agcaccggca agcctgcccg ccatgcgcct cccgccaaag ccgaccaggt cattgaagaa
     2101 ctccggcccg gccagtccat cgagttgctc caggagctgc acatcctcac gcgcgagggc
     2161 aagctcaacc aggacacgcg gcgcaagctc aagcaggtct accacctgta ccagttcatc
     2221 gcaccgctgc tggaggaggt ctccgccaag ggccggccgt tcaccctggc cgaccacggc
     2281 gccggcaagt cctacctggg cttcattctt tacgacctgt ttttcaatgt cgcgcatcct
     2341 ggcgagaaga ccggtggcca tatctatggc atcgagacgc gtgccgagct ggtggaaaag
     2401 tcgcgcacgc tggcgcagaa gctgggcttt gcgcgcatga cgtttctgaa tctcctggtg
     2461 gcggaagcca cggtgtcgga ccaattgccg gcgcaggtgg atatcgtcac cgccctgcat
     2521 gcctgcgata ccgccaccga cgacgccatt gcctttggcc tgcacaagca ggcgcgccac
     2581 atggtgctgg tgccctgctg ccaggccgag gtggccggcg tgctgcgcaa aaacaaggcc
     2641 ctcagtttgt cgaagacgcc gctcgcggaa ctctggcgcc acccgctgca cacgcgcgaa
     2701 ttcggcagcc agatcaccaa cgtgctgcgc tgcctgcagc tcgaagccca tggctacgat
     2761 gtcacggtga ccgaactggt gggctgggag cattcgatga aaaacgagct catcattgca
     2821 agccggccgt cagcgccgaa tgctgcaaag acgcggcagg cacaggcgcg gctacaggag
     2881 gtgctggcag aactgaacct gggtgaactc acggcgcgtt ttgcttcacc cgcctgagcc
     2941 tggtcgccgg aagtggggat gctctggtga cggcccgacg ccgggaagcc ctgttttccc
     3001 tattcgggag cggtaaatag tgttttttta aggttctcgt aggcattaat tagcgatgtc
     3061 ctcaggcgtc cctttctaga cttcctgcct ggctttctga tggctggcgc gtggtgcgtc
     3121 atgccggtca agccgcaagc aggccttgcc tgggaaaaaa agacaccatg aatatccacc
     3181 cacatgagat gacgcagctc gccgctgcac aaccgacctc ccgcctggat ctgtatgccg
     3241 gcatccacaa ggcgctgcgt gcgctgatgg ccgatacgct attggccgtg gggcgaatgg
     3301 acaccgatga cgacctggaa ctggccacaa ccacgcagcg cgtgtgtgag ttgctggatc
     3361 tttgcgcctc gcatctggcg cacgaaaacc agttcattca tgcggccatg gaagcgcgtg
     3421 cgcccggtgc cagcgacgtg atcgcccatg aacacgaaga gcatgagcag catattgccg
     3481 gcctgggcgc gctggctggc gccttgctga agtcgccgct ggcgggacgc gcaggcctgg
     3541 cgctggagct gtatcggcaa cttgcgcttt ttatcgcagg caatttccag cacatgcatg
     3601 tggaggaaac ggcgcacaac gcggtgctgt ggtcgcgtta taccgacgca gagctggcgg
     3661 acattcacgg cgcactggtc gcttcgattc caccggaaga gatgatgtac accctgcgct
     3721 ggctggtgcc cttcatgaac ccggcagagc gccgggccat gatgggcgag atgcaggccc
     3781 atgcgccggc tccggcgttt gccgcggcca tggacacggt gcgcccgcat ctgagcgacg
     3841 cggagtgggc gaagcttaca cgcggcctgg ggctggcggc tgcgcctggc ctggtcgagg
     3901 cttgacccgg caggctcagc cctggacttc cagcaggatg agcgggcgca tcacctgtag
     3961 ccggaagcgg ccgcgcccgg ttttctcgat acgaatgtgc gggccatggt caatgagccg
     4021 acgctgcagc agcagcagcc gggcttccag gttgtcggtc acatcgggca gctgaatggc
     4081 cgggtccagc cgcaactccc gattggaaaa ttccgtgcgt ccatgccggg catggtcgct
     4141 ggccagtttc cagaaaatgg cgccggccac gcccttgatc aggtaactgt cattcacaaa
     4201 gacgctgtcg ttggcgctga accgtcgcag cctcagggcc gtgccggttg cgggtggaac
     4261 ggggggtgtt gcaggcaggg ggaaggccgt atccagtgtg tcgggccggg cctgcatcag
     4321 gtcgatggcg gcgcccagat gcccgcccaa ggtgaccagt gcatcctcgt cctcaaagcc
     4381 gaaacgcagg tcctccgggc tctccgcgaa cagcacgccc agcagcctgc cggccgattc
     4441 aatgggtacg gccagctggc tgtgcggctc ggccagtccg ggataaggaa tgtcggtgtc
     4501 cagcagcagc ccgggcgcat gatcgcgcag gctgctgcga atggcgtggc tgtagaggtg
     4561 ggcactggtc atgtgcgtga tacgtacagg cgtgcgttcc cgcgctgcca cgccaatcac
     4621 gccattgccc atctcgattt cagagcccac gcccgacacg gcataaccgc agctggcgac
     4681 ggtgtagagc cgctgggtgc cggcgtccag catcaggacc atggcgtggc ggatgtccag
     4741 aaagtcgacc agggtggcga gggttgtatt gagcaactca tccagcccgg tgcagcgcgc
     4801 caggcgctcg ctgccgcggc gcagcgccgt gagtacattg gcgcgcggtg gcaaggccgc
     4861 cagtggctcg ccagcaatag gctcgatctc atgcacctca tagatgtccg aaccccgcag
     4921 cttgaagaca gtcgccatgc cactgtgtga ggcgatgccg gccagttgtg ctttcatgcc
     4981 ttcaaacacc gggccctgcg tctccgtgcg caggtagcgg atatgcaggc gaaagaatgc
     5041 cgccgtcacg ggatgcaata ccagcacggt ggcatgcggg ttggccagga tgttctgccg
     5101 ggtcttgttg aaaaactgga aagacagggc gacctgtcgc tcgcccgcgt agaacacctg
     5161 tgagatgtac gcgacgttgg gggttccatc gagagcgcag gttgccatga cggccgggat
     5221 cgcgccttcc aggcagggac ggatatcggc aagggtaggc gtgatcatgc tgcgcttgcc
     5281 ggcctggggt tcagggccgt tcctgccctg gggccaggcg tctggtcaaa agcctgctgg
     5341 ggttcgaaac tgatggccac cacgtcctcc agccgatggg ccagcatggc ctgggtgaat
     5401 gcagggccgt accccacccg tccgacttcc tgctccatcg acgccaggta gcgcgccagc
     5461 gcggcttcgt cggagggctc tgcacagcgg atgcgggcgc cgctggcctt gagctggacc
     5521 gtgcggtggg ttgccggctc tgaaaacacg acggcgatga ggccggtggt tgcaatgtcc
     5581 tgaatcagct ggcgggactg gggtcgcgac acgtagactg tgaccgtctg gcagccggct
     5641 gtgatggcgc tgcccaccgc ccgcatgatg ctcggtcgca gctcgaggct gcaggagctc
     5701 acgttgacag acacgccctt gtccatcatg gcgatcagct cgggcgacag cagcgaggcg
     5761 ggcgtggagg cgttcatgcc gccaaagtat aaaagcgtca ggcggggcgt gcagctattt
     5821 tcagctccgg aagcgcctgc gcgtcaagga caccaatacc acgattcagc atctctcaaa
     5881 ccggcaacaa ttcacaatgc gcagggcggg acgggcggtt aaacttcgtc cgcacttcaa
     5941 acccgggagg aatgagggcg ttcataaatc aggggattat cttgaaaaaa ctgcttgttc
     6001 tgctttgtgc gtgggcgctg gttggatgtg cgtcgattgt caacgactcg acgcatcctc
     6061 tcaaaattga aacgaagaca gcttctggaa cagccatcgc gggtgctgac tgctcaatca
     6121 gcaacgatta cggttccacc agcgtgaagt ctggtaccac gacgtcggtc cggcgctcct
     6181 ccaaggacat ggatatcacc tgtgtccagc aaggccagcc ggacgccacg ggtcgggcga
     6241 tatcgcgcgc aaatgccggc atggccggga atattatttt cggtgggggt atcggggcaa
     6301 tcatcgatca caacaagggc acagcctata cctacccgac ctgggttcag ctggtctttg
     6361 gtcagacgct tgtgtttgac cgcagggatg aaaaagacgg tgcacctgtt gggggtaaaa
     6421 cccctgaaat ggccgcccaa caacagcctg cagccactgc ttcaatgacg ggctcgactt
     6481 caacggcctg ttcccagggc gccggaccct gcaggtaagt tatctccaga tcaggccctg
     6541 aaccgccggc gcgtcagcgc cagcgccacc cagaagcccg ccacggtgta gcccaccagc
     6601 accagcccat gccgccacgg cgaagccggc cactggtcca tgaacatggg gcggatgatc
     6661 tccaccgcgt tggtcagcgg caaccagtcg gagatgaggc gcaccagcgg cggcaactgc
     6721 tcgcgcggaa agaagatgcc gctcaggaac atcatgggcg tcaacaccag cgtgaagtag
     6781 taggtgaaga agtcgtagcc cttggccagt gcgttgaaga tcagcgctat gcaggaaaac
     6841 atcatgccga ccagcagcag aatcggccag gccagcagca gcctggggct gtggctgatg
     6901 cccagggcca gcatcacgcc gaggatggcc gtcacggtga acagcgcctt gaaggcggcc
     6961 cagagcattt cggccagcag cacgctgtcc aggctgatcg gggcattcat gatcccgtcc
     7021 caggttttct gtacatgcat gcgcgaaaac gccgaataca gcgcctcgaa gctggcggcg
     7081 ttcatggcgc tcatgcagat cgagccgctg gccaggaaca ggatgtaggg gactttggct
     7141 gcgctgt
//
LOCUS       DAIDFG010000163         6962 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_163_length_6962_cov_16.348632, whole genome shotgun sequence.
ACCESSION   DAIDFG010000163 DAIDFG010000000
VERSION     DAIDFG010000163.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6962)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163_length_6962_cov_16.348632"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1169 a         2356 c         2268 g         1169 t
ORIGIN      
        1 acccaggcga tggcgctgat gtgcggctcg tcccactcct caaaaaacac gcggggccgc
       61 cgcttcaggc cggccgcagc ccgggcaatg gcggccagcc tgtcctgcat cacctgcatc
      121 agctgcaggc cgcgatcgcc ctgcccgacc atcgccgcca cctggtacat catggagaaa
      181 atttccgcga tgctgcgctg gttgaacacc gtgacctgca cgcccttgcg gatcagctcg
      241 gccgcaatgt cggcctgcag gtccgagaag ccgaacacgc agtcgggctt gagctccaga
      301 atcttgtcga ttttggcgct ggtgaaagcg ctgacacggg gcttctcggc acgtgcccgc
      361 ggcggccgca ccgtgtagcc ggagataccg acgatgcggc gctcttcccc gagcaggtac
      421 aaccactcgg tggtttcctc ggtcatgcag acaatgcgct gcgggccgta gttcatggct
      481 gcggccccga tgaaaaaagc cgggctgtcg cttccgggct ggacgcgaac caggcgtgaa
      541 aataactggc gcggaccgaa cccgagacat acagggcctc gccctcgccg cgcagctggc
      601 gccccggcgc cgcttcggtg cgggcggcgg gctgcagcgg tgtggtgcag gtcgagtagt
      661 ggaaggtgtg accgcgcagg gttccaccgt ccacgccaag ctgctgcgga cccagcgcgg
      721 ccaggcgttt ttgcatgctg acgctgccgg gcaacacacc ccacatcgga tggcgctggc
      781 catcgacggt gtgcagttcc tcaaacagcg ccatcatgcc gccgcactcc gcccagaccg
      841 gcttgccggc acgcacatgg tcggccaggc tgtccttcat ggcgcggttg gccgccaggg
      901 tctgcgcatg cagctcgggg tagccgccgg gcagccagag cgcgtcgcag tccggcaggg
      961 ccgcatcggc cagcggcgaa aaaaacacca gccgcgcgcc cagcgcgcgc aggcaatcga
     1021 gattggccgc gtaaatgaag cagaacgccg catcacgcgc cacggccacc gtgcggccct
     1081 ccagtgcacc ggccggaaac ccgggcagat ccggcgtctt gaacggaacc gcccaggcct
     1141 gcaggtcgtc cccgctcatc tgccccagcg gggtggcggc cagcgcatcg gccgcggcgt
     1201 ccagccgctg catggcgtcg ccgagttcat cggacatcac caggcccagg tgccgctcgg
     1261 gcagcgtcat gacactgctg cgcatgaggg cgcccagcca gtgctccggc tcacgcaggc
     1321 tgtcctcgag catcagcgca tggctgtcgg aggctacccg gttggcgagc acgccggcaa
     1381 accgcaggcc gtcccggtaa tgctgcagac cccaggccag cgcgccaaac gtgccggcca
     1441 tggacccggc gtcgatgacg gccagcaccg gcaggccgaa acgctgcgcc aggtcggccg
     1501 cgctcggctg cccgtcgaac aagcccatca cgccttcgac aatgatgagg tcggcctcct
     1561 gcgcagcggc ctgcaggcgc cgggcgcagt cgtcctcgcc ggtcatccac aagtcgagct
     1621 gatgcaccgg cgcgccactg gccagggcca gccagtaggg gtccagaaaa tccggcccgc
     1681 acttgaagac ccgcacacgc catccctggc gcgtgtgcag acgcgccagc gcgcaggcga
     1741 ccgtggtctt gccctggccg gaagccggcg cggcgatgag gacagcagga caggaaggtg
     1801 cagacatggt gttttccagc cggcttactg cggcgcccac ttcaggccga catagaggct
     1861 gcgccccgcc gtcgcataag tgttggcaag ctggtaggtc ttgtcggcca gattgtccac
     1921 ccgcgcgagg aaggtccagt ccttcgccac cgacgtgctg gcatggaggt tgatcaggcc
     1981 gtaacccgcg agcaccacgg tattgctggc actgtcattg cggcgtcccg aaagctgggc
     2041 ctcggcgcct gctgtccatg aagcgatgtg ggtgtcggcc cccaggctgg cgtgatgccg
     2101 ggcgcggcgc gcgagctgcc tgccggtcga aagatcgcgg gggttctgta catcgagcga
     2161 ggcatgcagg ttgacctcgc cgagtttgtg ggtgcccgag aaagtcaccc cggcatattc
     2221 ggcgcgggcc gtgttggaat agcagcccag accggacgcg cagcgccccg gggtggagaa
     2281 ggtgatgagg tcattgaccc tgttgcggta caccaccacg ccgaagctgc tggcgccttc
     2341 ggcataacgc agacccagct ccaggttgcg gctgctttcg ggttgcaggc tggacacgcc
     2401 atagacgctg aaacgctggt aaagcgtggg tgccctgaaa gcggtgccgg ccgacgcggt
     2461 ggcccgccac tgaggcgtga aggcaaagcc ccaggcagcg ctgccggtgt tgtgatcgcc
     2521 gaactcgctg tcctggtcgt tgcgcaggtt cagctgcagc gtgtgccggt cgctgcggta
     2581 accataaccc agggccagtg catcctgcga acggctgcgg ttgatgggcg cattttccag
     2641 atgatcgact tttcgctcga gcgcggcggt cagcaaatga ctgcccagac gaagctcgtt
     2701 ctggaacagg tagccgttca gctgcgtggt ggtcaggtag ggcgacggcg tggtttcgta
     2761 gcggtcgcgt gagtcggtca cgctcagctt cgtgctgtag gcctgcgtcc attgcgcctg
     2821 ccagttcagc cccagcgcct gcaggctgtg caggttgcgc tcgtcgacga accttgtggc
     2881 actgtcgtat tgcgagttca tgtcgcttgc cagcgccgtg acctccagcc ggtgggcggg
     2941 atcgacttgc aagccgatgc gggcgttggc cgacctgctc tcgtagccgt cccggtcggg
     3001 attctggccc gctatcgggc gcgagttgaa acccttgctg ctttcgtcgg acaggcccag
     3061 cgaataatcg aatgtgccac tgctgccgct gatgctggca tcggcttttt gcgtgccgtg
     3121 ggacccaaag ccgatgccga ccgccggcgc cggcggcccc tcgcccctgc gggtgaacag
     3181 ctggatcacg ccacccagcg catcggaacc gtagacagca ccggccgggc cgcgcaggac
     3241 ctcaatgcga tcgatctggc tcagcggaat ggtctcccag gccgcgccgc cggtggcctg
     3301 cgaatcgatc cggacgccgt cgatatacac cgcggtaaaa cgtgactcgg cgccgcgcag
     3361 gaacacgctg gtggtgctac cggggccacc gttgcgcgcc atctcgatgc cgggcacacg
     3421 cgccagcaca tcggccaggc cggcagctcc gctgcgttct atggcggcgc ggtcaagcac
     3481 ggtgacatcg gccaccagat cggaaagggg ctgcgccgtg cgtgtcgcgg taaccacggt
     3541 ctcccgcagt tgcggggatg aagcggtttg ggaaaaagca ggccatgcgg cagcgaccac
     3601 cagcgaaagc gcggcggagc gtgcatgtaa tacacaggtt ttcatgggga gaaacattca
     3661 acaaaaggcc ctcaccggct tccccgccag tgcatgagcg ttaaaaacgc ccggtccaat
     3721 gaaagacgcg cgcgttcacc ttgttggccg gtatccgggc tgacggaact gctctcatcg
     3781 ccttcccaag cgcgtgttca gaacgctcag tggctgatga tgaaagctga atgtgctccg
     3841 agtctgaatc tggggagcga cattcgtccg tttaccgttg cgggggcagc gcaggttaaa
     3901 aatccagtcg caatcggccc gggctgaccc ggtgcggatg gccttgattc cctcctgctt
     3961 cccgttgaac tgcagcgtgt gaaccacacc gcgagcacca acagacaaga ttctacgcgc
     4021 caaaaccgtg caaaccctac aattggcgct gccatgtcga accccaccca catcgaccag
     4081 attgccgaac gcgtcgagcg gctcctcttg cgctacgagg agttgcagcg caccaatgca
     4141 ctgctcgccg agcaggtgca tgaactcacg caggaacgcg attcgctgcg ctcgcgcctg
     4201 agcgcagccc gcgcccgggt cgatgccctg ctcgaacgcc tgccggattc gtcggcgccg
     4261 atgtcggcag ccggtccggc ccatggcagg acacccgtgg gatcggattc atgaagcagc
     4321 ttgacgtaca gatcatgggc cagagctacc tgctcggctg ccccgagggc ggcgaggcca
     4381 ggctgctcga cgcggtcgaa aaggtcgacg cggccatgtg caagatccgc gacgccggaa
     4441 aaatcaaggc gcgtgaccgc atcgccgtgc tggccgcgct gaacctggct ttcgagttgt
     4501 cggagatgga ggccaatgca ctgaaggcgg cgcacagcat cccggcgccg gccgctgccc
     4561 cggccaggtc ccacggcgcg ggcgtgtcgg cggccgaagc cgcgtcgcag ccgcaactgg
     4621 cggccttgct gcaacgcctg gacaacgcgc tgggcgtgga cggccgcctg ctgtaaagcg
     4681 gcctatcggg ccagcgcggc gcccgccaaa cgcctacaat caaaccgtct gcggtacaca
     4741 ccgggcttta tatttccttg taccaatgct cttagagcac gggcttggta aattgtcagg
     4801 caggcgtgat cgtctcgcgt tagacgaacc cgaaacctga ctgccctcgc ccacctgaac
     4861 cctggttcag gatgacggcc cggtggtatt cgcagacacc tcttttcacc cgtcaccacc
     4921 acccccgccg ccattcccgt ccaacccgct tgctccggca agcagcctgc ccttacgccg
     4981 catgaccttc gaacccctgc tcattcttga acttgccctg ctgggcgtcg gcaccggctt
     5041 tctcgcgggc ctgctgggca taggcggcgg catgctgatg gtgcctttca tcaccatcat
     5101 catggcgcag cgcggtgtgg cgccggacct ggccgtcaag atggcgattg ccacctcgat
     5161 ggccaccatc atcttcacct ccatctccag cgtgcgtgcc caccacaagc gcggcgcggt
     5221 gcgctgggac atcgtccggc gcctggctcc cggcatcgtg gtcggtagca tcatcggcag
     5281 cctgggcgtg tttgccttgc tcaagggctc ttacctggcg atcttcttcg gcctcttcgt
     5341 gagcttctcg gccacccaga tgtttctgga caagaagccc aagcccacgc gccagatgcc
     5401 gggcacggcc ggccagtttg cggccggcgg cggcatcggt ttcctctctg ggctggtggg
     5461 tgcgggcggc ggcttcatca gcgtgccttt catgacctgg tgcaacgtcg ccatccacaa
     5521 cgcggtggcc acctcggccg cgctgggctt tccgattgcc gtggccaatg tgctcggtta
     5581 tgtgatcagc gggcagtcgg tgcagggcct gccgcccgct tccttcggct acatctggct
     5641 gccggcgctg gtggtcattg cagcctgcag ttttttcgtc gcaccgctgg gcgccaagat
     5701 tgcacacaag ctgccggtga gcaagctcaa gcgcgttttt gccagcatcc tctatgtact
     5761 ggcggcctac atgctctgga agggcctgac gagcctgtag cgcccgccgc cggtcatgga
     5821 ctcagccatg cgcccggcgt gaatcggccg gcgcctcgtc cctctccctg agcccgtagt
     5881 cgcggatcac ctcggcgatc cgcaggcggt agtctttgaa catcgtggac cggccggcag
     5941 cctgcgcggc gcgatgcgcc tccaggttgc gccaggcctg caggcaggac tcgtcgcgcc
     6001 agaactgcag cgacagcagc ttgtcgggct cgctcaggct ctggaaacgt tcgatcgaga
     6061 tgaacccgtc catcttcagc aactcggcct tgagctcggc ggcccagttc aggtaaccct
     6121 cgcggtgcgc cgggtcgggc cagacttcaa aaataaccga caccatttaa tttctccttc
     6181 attgctcgaa aacaggaatg cgcgcattgg gcagtggccg gaacccgctg cagcgcccga
     6241 aatactgatc aaacaggcct gcagccctca tccataaagc gcaggcagct accatttcaa
     6301 tagcaatcgc agcgccgggg caggcatggg gtccggcacc gaagcccatg ctcccgcctc
     6361 cttcacggtg tacatcaaaa cagtcgggct gcgtgttgaa ggtgtcgtcg cggttggcgc
     6421 tggccagcac cagcagcagg gcctggcccg ccgctatcgg ctggcccagc agtgtcagcg
     6481 cttcggccgc gaagcgccgg gtgttctgga tgggagcggc ccaacgctcg acctcgcgca
     6541 ccagggcgcg catggcatcc cgtgacgcgt cggcggccgc cgcctgccgc gggtcctgcg
     6601 cgagctgcag cgcgacatgg ccgagcaggc cgctgcccgc atcgagggcc tgctgcatga
     6661 aggcgatgcg gttggcggcc tgcaccgcag acaggcccag cgccctgccc tggtccatca
     6721 gggccgcagc cgccgcgtcg gccagcgcca cggcctgcgc gtcggcgccg ggcgcgatgc
     6781 cttgcgcgaa ctgcgcggcc cagcggcagg tggcctcgcg ttcgccggca ggcacatcga
     6841 gcagccgcgc catggtctgc accggcaacg cggcgatgaa cgcattggcg ggcaggcgcg
     6901 gccgcaggtc gcgcgccgcg tcttcggcgg cgcacgccac gtcagccatc gtccagcgcc
     6961 gc
//
LOCUS       DAIDFG010000164         6947 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_164_length_6947_cov_17.051364, whole genome shotgun sequence.
ACCESSION   DAIDFG010000164 DAIDFG010000000
VERSION     DAIDFG010000164.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6947)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6947
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164_length_6947_cov_17.051364"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1192 a         2106 c         2317 g         1332 t
ORIGIN      
        1 tgattgcgct gtcacagctg agccgcggcg tcgaatcgcg caccgacaag cgccccatga
       61 tgagcgatct gcgcgagtcc ggcgcgatcg agcaggacgc cgacatcatc atgttcatct
      121 accgcgacga gtactacacc aaagacgcct gcaaggaacc cggtgtggcc gaggtcatca
      181 tcagtaaaca gcggaacggc ccgaccggga ccgtcaagct ggcgtttatc aaccgcatca
      241 ccaagtttga gagtctggcg catgggggcg gtggggattt ctaaggcaag cagcctgtgc
      301 cacattgact gtcaaaaacc tgcgggacat ttaacgtaaa cagtgaaaag ctcgatgcaa
      361 gaagcaaact atccagcgct tatcctatcg atggtttcgc tattgctcag tgtccttacg
      421 ttctactgga cgacgcttcg caagcgtaaa gctttttatc ttttgcgaac atcagggttc
      481 atgaatttgc aaccgcagtt cgttatggtt aacggtggga aaagtgacct actggtcact
      541 gaaatgattt gccgatttga gcatgctgca cccggaatcg gatcagttcc gaaacaaaaa
      601 atcgcctttg aaaacggaag cgattggtta ttgagttctg gcaaagcccg tcatggtttc
      661 gttgaattta cagagccttt tgatggtgca tttgccaagc aaggacggcg tgatcccaag
      721 aagatgaatt tattcttttt tgatgtgtat ttggatatta aatggattga tatggatggc
      781 ttgactcacc aaaaatcggt tcaactcact cagtatggac ttgacgaacg cggggtgatt
      841 acgatgggtg gtctggagga ctttagctac aacatgaatg acagcaaagt tcgcggtatc
      901 tgggctgccc gctccttttg gcataaaaag ccgtagcttc aaatattggg ttcccagcct
      961 ctgttcccaa tcaattggcc tccattcgct aaatgagaag cctcttcatc tccaagagtt
     1021 agcttaacgc ggcttcgcca ccatcaaata aaacaccccc agcatcgcca caatcgcggg
     1081 gtagcccagc cattcccacc agcgcgcata acgccagtag gcgtccgcga aaacaaacaa
     1141 cgtcatcaag accaccagtc aaggtgtgct cagtggcagg tccgctagat agatagatag
     1201 acacgccatg gcaaagcccg tgacgggttg aatgaccacg gtgggtttag tgagcttttt
     1261 gcctgtagcc caataagcac gggcgcagca agctattgat ttcgtagcgc cttcgggaca
     1321 ggtgagccgg ttattccaga tcttccatgg ccagcatgtg gtacttctgg gcataggaaa
     1381 aaggctgctt tctcaccacg gtcatcacac gttctgtctc cttctcggag ggggcatgca
     1441 ccatcagggc gtgctggcct tcgcgggcca gggtgacgta tttctggact gtggcgcctt
     1501 cggtgcctat cgatggcagc gcgacatcgg agtcgccatc gaccttgccg atctcgcgca
     1561 ggacgtcgtc gggggtcagc agcatgatgg cctgcccgtc gaagccctgg tcctccagct
     1621 cacgcgcggc ctgctcggcc tgaccggctt cggggaacat gatgaaggcg taacccgtgg
     1681 ggtagaacac gccctggagg gtcaacatcc tggggtggac tgcaaatgtt ttcatggtga
     1741 atccttgggc tgtgaatcca ccatttatgc acgccagcgg cacgctgtct gtaggccagt
     1801 tcgtacaaag cgcgtggcgc ggcgccccgg aaaggcgcgg cggttcaggg cagttcttcg
     1861 gtccggaggg tgggttccga catgacgcgg gcggtgctgg acaggctgtc gagcagccgg
     1921 ttcgcaaaga agctgctttg cgtgtccggt tccagcgcgg cgatcacgag gttggccagc
     1981 ggctggttca tgggcacgta gtagctgccc gagggcgcgt cgatcacgcc gcgcaccagc
     2041 gccacgtcga ccttgacgat ctgcgcggca tcggcgatgc tgccgcgcac atcctgccgg
     2101 accccggccg agctcgccgt ttcgcggtac aggtcgccca gcatggacgc cggctcggcc
     2161 acccgcagca cctgcacacc atgcaggcgc aaccgctcca ccgccgttcg cgaccccgcc
     2221 gacagccagt agccgcaggg acgcacgcgc gtcttgatct tgcgcagcgt gagtgccgag
     2281 ttccattgga ccttgagggc cttgtcggca ccggtgaccg ggtccagcat gacgagttcc
     2341 tgctgggtga gggtggggcc ggcttcgacc acggcctctt ccttgcaggc cagggcgctc
     2401 atctctttct cgacataagg ctgcagctgg cccagttcgc tggcccgctg ggcagtgctg
     2461 gtgagcacgc tggagatcgc ggtgacatgg gtgtgcacgc ggcgctggat gtgcagccgg
     2521 cctatgccca cgccacgggt ttcgatcagc aggctgaccg cgtttttcag gccgctgacg
     2581 ttgcggccgg tgtcgggctg cgtgccgccc atcgagatgg tcttgtccgc caggtctgtc
     2641 gaggtggtgt agtaccactc ggtgctgagc ccttgcctct tgagggcggc caccagcggt
     2701 cggcggtacc actcttcgga ggctttggtc aaaaactcgg ggatgttggc ggtcgtcgcg
     2761 tactgcagca gggcgtcgta cttctgcacc gtgccgaatt tctgcagaaa acggccaacc
     2821 acggtgtatt cgtgggcatc aaccacgacc gtcgggtggt agtcgcgcgc gagccgggcc
     2881 agggcctgtg cttccggtgt gttgagcagc aggtgatcgc ggttcatgtc caggccgccg
     2941 gtggtcacgc gtttgttgtc catcgagcca tcggggttgg cgcgcggcac gatgatgacg
     3001 ttgacacgat cgagcaccgg ccgcagcagg ccttgcgtga gctcgcgggc cacgaccagc
     3061 aaggcttcgg tgccggcggg ttcgtcgcca tgctgctggc cgatcagcag cacggtgggg
     3121 cgggccgagg ccgcgatggc ggcggcatcc gtgccggcgg cacgcgtcag caccagggct
     3181 tccagggtct cgccgcgttg tgactggcca atgggcagca cggcggcttt caggcctggc
     3241 gagcgcgagg ccacggtgct gagctcacgc agccaggtct ggatttccgc attcgaggtg
     3301 aagctgccgc gtccttcctg caggccgggc gtgttgtaaa tgacggaagg caccgggaaa
     3361 cgggcggcga cggcggcgct gtagggggcg gcgctggcag gcgaggggcc catcgccggt
     3421 cggtcgggga cgggctggac gacaacgccg ggaatggggg cgggcgccgg cgccaccacc
     3481 acccgcggcg gggccgtgac cgtgccggtg ggcgaaggcc agggcggcag cggtaccgag
     3541 ctacaggccg ccagcgccag cagcagcggc agcagaacga cggcgcgtgg gccggtcccg
     3601 cgagggcgca ggacaagggg cgatgggcgc gatggttccg ggggactgag ggaacgggtc
     3661 atccgcaggg gcaggtcggt cttggtcatg gcttggtgtt gggaaggcag gcacaggtgc
     3721 ggcccggact gccattatcc ggcttgcgcg cctctgccgg tgttggtgcc taccctctgt
     3781 gcgctctggt tcggcatttt tgccacccgt tcgcggcgtc gggcgggccc gcatgagaaa
     3841 ctacggcttc catgctctcc gaatccgctc cttccaccat gaatcccggt tctttccccg
     3901 cacatcgcct cagcgtggcc ccgatgatgg actggaccga ccggcattgc cgctattttc
     3961 accgcctgct gtcacgccat gccctgctct acaccgagat ggtgaccacc ggggcgctgg
     4021 tgcatggcga cgtggcgcgg catctgcgct tcaacgccga agaacacccg gtggcgctgc
     4081 agctgggcgg cagcgagccg gccgatctgg cgcactgcgc ccggctgggc gagttatggg
     4141 gctacgacga aatcaacctc aactgcggct gccccagcga acgcgtgcag cgcggcgcct
     4201 ttggcgcctg cctgatggcc gagccgcaac tggtggccga ctgcgtcaag gccatggtcg
     4261 atgtggtgca cgtgcccgtc actgtcaagc accgcatcgg catcgacaag ggggagagtt
     4321 acggcttcgt gcgcgacttt gtcggtgcgg tcagcgaagc cggctgcacg accttcatcg
     4381 tgcatgcgcg caatgcctgg ctcaagggcc tgagcccgaa ggaaaaccgc gaggtgccgc
     4441 cgctgcgtta tgagatggtg caccggctca agcgcgagtt tccggcgctc accatcgtca
     4501 tcaacggcgg catcaccacc ggtgaacagg tggcggcgca attgcgtgaa ctcgacggcg
     4561 tgatgatcgg ccgcgaggcc taccacaacc cctggtggct ggcctcctgg gatgaggcct
     4621 ttttcggcgc ggcgcacagc gaggcgacgc gcgaatccgt cgaggcgcag atggccgact
     4681 acatggccag ggagtttgcg gaacacgaca cgccctggcc aaccatcgcc cgtcacatgc
     4741 tgggcctgcg ccacggcctg cccggttcgc ggcgctggcg ccaggtctgg agcgaccaca
     4801 agctcaagag cttgcacccg cgcgaggtga tggcactggc ccatgcgcca ggcgctgcgg
     4861 gcgaaagcgc tgaatcttca tggcaggttg cggtgccggc gcatgcttga cgccgcgcag
     4921 cgcgagacgc gccgggcgct gctgctcctg tggctggtgc cggcgctctg ggccgtcaac
     4981 tacatcgtgg cgcgcaaggc gcccggcgtc atcgggccct acatgctggc cctgggccgc
     5041 tggggcctgg ccgccctgat cctgatggtg atcacgcggg ctgaactgtg ggagcaacgg
     5101 gccgcaatca cgcgggcctg gcggcaatac ctggtgctcg gcaccctggg tatgctgatt
     5161 tgcggtgcct gggtgtatga aggggcgcgc tccaccgggg ccatgaatat cgcgctgatt
     5221 tattcggcct cgccggtgct gatcgccctg ggggccatgt ggtggctggg cgagggtttc
     5281 ggcgcgcggc aggcggccgg tgtgctgctg gcgctgacgg gcgtggttca cgtggtcgtc
     5341 aaggggcgct ggacggccct ggccgacgtg cagtttgtgg caggagatgc gtggattctg
     5401 gcggccacgg tgtcctgggc ggcctatgcg ctgctgcaga aaaggtggcc cagttccctg
     5461 agtgcaacgg cgcggctggc tgcgatctgc ataggcggca ccctggtgct gctgcctccg
     5521 gcggtgtggg aggcgacacg gcccggcgca gacaccctgg gcagccaggc cttgctgctg
     5581 gtggtggtgg cctcgctgat tccgggcgtg ggtgcctact ggatctacgg ctgggcgcag
     5641 aaaatcctcg gggccagccg ggtggccgtg gcgctgtacc tgggcccgct ctacgcggcg
     5701 ggggctgcct ggctgctcgg cgaaccaccg ggctggcacc atgcggtggg tgcgctgctg
     5761 atcctgccgg gggtgtttct ggtcacccgg ccgatcaaac agcctgtcaa gccgcagctt
     5821 ccagcccgtt cctgaaatgc tcgcgcatgc gctgcacggt ggcatcggca tcgcgtgcgg
     5881 ccaacgccgc catgatggcg cggtgttcgg ccaatgactc ttcgatgcgg ccgcttttga
     5941 gcagtgagtt gtggcggttg agcttcatga ccttgcgcag atccgccacc atctggtcgc
     6001 gccagcggtt gttggcgatt tccagcaggc gcatgtggaa ggtttcgttg atctggaaaa
     6061 accgctcgcg gttggccacg gctttttcga gctcgcggtg cagggttttc agctcctgca
     6121 gctgggcttc ggtggcctgg gccgccacca cgcccgcggc gtcgctttcc agcagcgaga
     6181 gcaggtggta gacatcggcg aggtcgcgtt cggacacctc ggtcacgtag gccccgcgcc
     6241 gcaccttcat ggtcaccagg ccttcggtgg ccagcacctt gagggcttcg cgcagcggcg
     6301 tgcggctgat gccatattcc tcggccagct tgagctcgtc gatccagctg ccgggcgcca
     6361 gttcgcggct gaaaatgcgc tggcgcaaaa gctccgccac ctcttcgtaa agtgcgcggg
     6421 gggtcagtga aacagcggcc atggtcttgg tagggtgtcg gttgcagccg gagggctgga
     6481 cagcgcgatc ttatccgaaa ctaaattttg catcaataat tataaataat gtaaactcgc
     6541 caaatcgaca gcttgcctgc ccaactccat cgaagcacgc gccatgagca ccccaaaacc
     6601 ttcccccagc cctgaattcg cctctgccag tctggaggcc tgggccaagg cagccgccaa
     6661 atcggcgccc ggcggcgacc tcagcgcgct ggcttggcag acccccgacg gcatcagcgt
     6721 caagccgctg tacaccgcgg aagacgtcaa ggacctgcct tacaccaaca ccctgccggg
     6781 ttttgagccc ttcattcgcg gcccgcaggc caccatgtat gcggtgcggc cctggaccat
     6841 ccgccagtac gcgggttttt ccacggcgga agagtccaac gcgttttacc gcaaggcgct
     6901 ggccgccggc gggcagggtg tctcggtcgc gttcgatctg gcgacgc
//
LOCUS       DAIDFG010000165         6927 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_165_length_6927_cov_16.393772, whole genome shotgun sequence.
ACCESSION   DAIDFG010000165 DAIDFG010000000
VERSION     DAIDFG010000165.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6927)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165_length_6927_cov_16.393772"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1340 a         2233 c         2266 g         1088 t
ORIGIN      
        1 atgcggttgc cttccatggc cgtgaccatg ctggccgttt cccccggcgt gttcagcaaa
       61 atggaagtgg tcgagccacc atacatggca ccgtaataaa tacccgcaaa aaagatcatg
      121 gaggccgtgg gctcgaccct ggccgtgatg ggcagcagca tggccaccgc gacggcgggc
      181 ccgatgccgg gtaggacgcc aatggccgtg cccaacgcac agccgacaaa acaccagagc
      241 aggttgaccg gtgtggccgc agtggcaaag ccctgcagca gctggttgaa gatgtccata
      301 gaacctggaa gtgtcagttg aacgcgcccg agtgggccgt catggggtgg gctgggtagg
      361 cgtgaggacg cggcaggcaa acgcctccaa ggacgagcaa cgcccccagg tcgcgccagg
      421 gcggttcaat cgggtgtgta gcgctaccgc gcaaagcaat gcggttggaa aatcagggtg
      481 ggcggcattc atacacatcc tttagcacca cgggcggtca actgacattt ccaggttcac
      541 agccagccgg tggaggtgag gcccggcagg ttgatcgcca gaaattgagt gaaggtccag
      601 tacaccggtg cggaaatcgc gacaccgatc ccgacgtcct tgagccaggc gaccggtcgc
      661 cgatctgcct tgccttcggc accacgcagc ccctggaccg cgagcacaaa acacaaggtg
      721 caactgaaaa tgaagccgat ggtggtgatc agcgcggcgt tagccagcaa acccgcagag
      781 acccatgcaa atccgaccca gtcgccatgg gccgcaccac cgggctcatc cagggagcgg
      841 aagccgccgg tacgcgcttc ccagacaata aaggcgccgc aaaccagcaa ggccgcagac
      901 atcagccagg gcaggaagtt cggcccgacc ccgccatagc ctgccgcaga cgggatgctg
      961 acggcgccgc cggccagccc cagggcgagc agcacgatgc cggcaccgac cacggtctgc
     1021 agacggattt gagcagggga aggggttgct tgtgtcatga gttgtctcgc ttgaatgcgg
     1081 actgtcatcc cggacttgat ccgggatcca tgcacatcaa ctgcagcctt gcgtgcgcga
     1141 acatggatgg cggcacaagg ccgccatgac aaaccgaaaa ggcaggtatg ccggttgctt
     1201 tagaccatgc cagacttgac catggtggca cgcaggctgg cgaagtcgtc gtcgacaaac
     1261 ttctcgaaag ccgcgccggt gagcacggca gccgtccagt tgtttttctc ggaggcttcc
     1321 atccagctct tggatttgac ggccttgaga atcatctcgg tcagtgtctg gcgctcggca
     1381 gccgagatgc cgggtgcacc gtaaacgccg cgccagttgc cgatcaccac attgatgcct
     1441 tgctccttga gcgtgggcac gttgacaccc ttgaggcggg catccgatgt gacggcgagc
     1501 gccttcattt ttccggtgtt gatgtattcc gcaaattcgc tgtagccgct gccgcccacg
     1561 gtgacgttgc cgcccagaat ggctgccgtg gcttcaccgc cgccccggaa ggccacgtag
     1621 ttgatcttgg aagggtccac gccgacttcg cgtgcaatca tggcggcggc gatgtgctcg
     1681 gtggatccgc gcgaaccgcc accccacttg acgctgcctg ggtctttctt gagctgcgcg
     1741 atcacgtcgg ccatggtttt gaacggcgag ttggcgggca gcacaaacac gttgtactcg
     1801 ctggtcaggc gcgcaatcgg cgtgacctgc gacaggctga ccggcggctt gccggtgatc
     1861 aggccgccaa gcatgacggc acccatgacc atcagcgcat tcgggtcgcc cttgctgcca
     1921 ttgatgaact gggccaggcc gagcgcgccg gcagcgccac ccttgttgtc ataggtcacg
     1981 ctggatgcag cacccgattc ctgcagggcc ttgcccaagg cgcggcccgt ggcgtcccag
     2041 ccgccgccgg gattggccgg aatcatcatt tttacattgg cagcagccag ggccgacagg
     2101 gggaacgcac ctgtggcggc cagggcggcc agggatttca gaaaagtatc gcgacgcatg
     2161 tatgtctcct ttggtgttgg taacagagcg atgatgcgct tcctgtctgt caaacggctg
     2221 tccgaaagcc ctagggaaag tgctaatgtc ccgcccatgc tgaccaccat gaaattgctg
     2281 ctgatcgagg acgacccttc catgcaggca gcgctgcagc gcgccctggg ccggcgcggc
     2341 atggaggtgc tgggctgcac cgacgggcgc aaggcgctgg cgttatggac ggccgcccag
     2401 cccgatgtgg tggtgctgga cctgagcctg cccgggctgg acggcctgca ggtgctggag
     2461 caggcgcgcc gccaggggct ggccacgccg gtgctgatcc tgacggcacg cggcacggtg
     2521 ggcgaccgca tcatcggcct gaacacgggc gcagacgact acctgcccaa gccctttgac
     2581 ctcgacgaac tcgaggccag gctgcgcgcc ctgagccgcc ggcgcacggc attcggcagc
     2641 gagccggggg ccggcgacca cctggtgggc cagcttcgtt atgaaaagga aagcggcgcg
     2701 atttaccacc gcgaccaggt cctcgaactg acgccgcgcg agctggccct gctacaggcc
     2761 ctgatcgtca aacccggcca ggcggtgtcc aaggaaaaac tgttcgagct ggtgtttccc
     2821 ggcgagaccg atgtgcagta cgaggccgtg gaggtggtgg tgtaccgcct gcgcaagaaa
     2881 atggcgcaca ccggcgtgac cctggtcacc ttgcgcggcc tgggttatct gctgaaagtg
     2941 gacgcgccat gaaaggtgtt gaggacgacg tgcgcagcgc cgggccgccc caagcaagca
     3001 cagccccccc ggggggcagc gtattacacg aagtgaaaag cgtgggggtc aaagccttca
     3061 gcgccgggcc gcccccagca agcaccgccc cttcttccgg tgaaaggggc agcgcaacgc
     3121 cgcaggcaca cgaagtgaaa agcgtggggg ccatctcgct caggcgctac cttctcttgg
     3181 gcatcctgct gcctgtgggc ctgctggtca cgctcaacac ggccagcctg tacagccagg
     3241 cgctgggcgc cgtcaacacc gcctatgacc gcacgctgct ggcctcggcc aagtcgatcg
     3301 gtgagcagct ggacgtgagc ggctacgacg ggcagtccga actgcgcgtg accgtgccgt
     3361 atgcggcgct ggaagccttc gaggccgaca accagagccg gcttttttac cgggtgtcca
     3421 gcctcaatgg cgaaatggtg tcgggttatg cgcagctgcc tttctggcgc ggccgcattc
     3481 cggccaaacc gccctacgcg gcgttggtcg atttttacga cgatgtgtat gcgggcgacg
     3541 ccgtgcgggt ggccgtcctg ctgcagccgg tggccagctc gaacgggcgc ggtatggccg
     3601 tcatccaggt agccgaaaca ctggaactgc gcaccacgct ggcgcaaaaa atcctgttcg
     3661 acaccctgtg gcgccaggcg ctgctgctgg ccgtgattgc cctggtggcc gtggtggtgg
     3721 tgcagcgcgc cacgcgcccg gtgcgtcgcc tcagcgccga gctgcaggcg cgccccgagg
     3781 gcgacctgac ggcgattgcc gcaccggacg cgccacgcga gctgctgccg ctggttgacg
     3841 ccaccaacga ggtcatgggc cggctgcagc acctgctgga caaccagaaa cgttttgtgc
     3901 gcgacgcggc gcaccagctg cgcacaccgc tggcggtgtt gaaggtacag gtgcagtcgg
     3961 cgctgcgcgg cgacatggac gcccaggcgg cgctggccga gatcaaccag accgtggacc
     4021 gcgccacgct gctggccaac cagatgctgg cgctggccaa ggtcgagcag ttgcgccagc
     4081 aggcggacct ggggccggtg gacatggacg ccatcgtgcg cgccgtcgcg ctggatctgg
     4141 cgccgctgat tgccgagaag gacctggact tcgatatcga aaccacggca gccagcgtca
     4201 atgcgcatga atggatgctg ggcgaactct gccgcaacct gctgcacaac gcgatcaagc
     4261 acagccagcg cggcggcccg ctgtcggtgc ggctcgtcac cgatgcccgg tacctggccc
     4321 tgaccgtcag cgacagcggt cccggcattt cgggcgaact ggcagcacgc ctttaccagc
     4381 ccttttcggc cggcaatgtg cgccacggct cgggcctggg cctggcgatc tgccgcgaga
     4441 tcacgcaggc gctcggcggc agcatcacgc tggaaaaccg cgaagaccat gggcgtattg
     4501 ccgggctgga cgccaccgtg cgcctgccgc tggtacacaa tgacgggcaa tgaaacacag
     4561 gtctcccctt ccctggcatg gcggcgcacg agaggtcaac catgcgcctt gacaagtggc
     4621 tctgggccgc acgcttctac aagacccgtt cactggcgag cgacgaaatc ggcaaaaacc
     4681 gcgtgcaggt caacggccag gacgccaagc ctgcgcgtga ggtgaaggcc ggcgacacgg
     4741 tggcgatgcg gcaggggccg gtcacgcgca ccgtgctggt ccggggcctg agccagcaac
     4801 gcgggccggc accggtggcg cagcagctct acgaagaaac gccggacagc atcacgctgc
     4861 gggaagcggc ggccgagcag cgccggctga accatgaacc ggccggcagt ctggagcacg
     4921 gccgccccac caaacgagac cggcgctcgt tggacaaagc ccaggacaaa gcctggggtg
     4981 accgctggag cgcctcggtc gactcctgaa acgctccttt tttgatagct gcctgcgccc
     5041 acccaataag ggcagaaagc cgatttcttt ataaggaagc agcagcagtg gctggcggca
     5101 ccggaccgct gccacggcgc atgcgccggc cctgggacca ccagaccagc agggccagta
     5161 acagcgcggg caggtagaac cagtgggctg tgggcctgtc ggtcggcacc ttgatggcag
     5221 tcacgtccca gccctgctcc acgccggcct tggcggcacg cgaaccgaat ttcgcctggc
     5281 cgatctgcag ggtgtcaccg agcggaacca gttgcaggcc ggcgtcggcc aggcgcttgc
     5341 ggccctccgg ccccttgtca ccgagctgga ccgccacggt cttgacgatc tcccggcctt
     5401 ccagcgtggt gccgcgcgtg accatcacca cgcggccgtc actttccacg ttgcgcgcga
     5461 tctcgaacac ctcggcgggt ttggcatcgc ggtattcggg ggcgatccgg tccatgaaaa
     5521 agtccgggcg gaacagcagg atcaccgcca acagcaacgc cagcgtttcc cacaggcgat
     5581 tcttgatgcg gaaccagttc atcgtgacgg cggcaaacag cagcgtggcc agcatgctgg
     5641 cggtcacgat gcagaaaaag gagaaggccc cgccgacatc gatcagcagc aattgcggat
     5701 tgaagatcca gatgaagggc aggatcaccg tgcgcagcgc atagatggcc ccctggatgc
     5761 ctgtttctat cgcgtcctcc cccgagatgg cggccgccgc gaaggtggcc agccccaccg
     5821 ggggcgtgat gtcccccatg atgccgtagt agaagacaaa caggtgcacc gcgatcagcg
     5881 gaatcaccag ccccgactgc gcacccaggt ccaccaccac cggcgccatc agcgtggcca
     5941 ccaacacgta gttggccgtg gtcggcacgc ccaggcccag caccaggcag gaaaacgcaa
     6001 tgaacagcag catcaccatg acattgccct gagacacaaa ctcgacaaat tccgtcatgc
     6061 gcagccccag gcccgtgagc gtgatcccgc cgacgatgat gccggccgtc gccgtggcca
     6121 cgccaatgcc gatcatgttg cgcgagccat tgtgaaaccc gtcaaccacc gaacgccagc
     6181 cctcgtgcca ggcatccggc gtgcgcgtac ggcgcagcgc gtcgatcagc gggcgctgcg
     6241 acatcatcag caccacgagg atggtcaccg cccagaaagc agccagcgcc ggtgacatct
     6301 cctcgaccat caggcaccag atcagcgtgc cgatgggaat gaggaagtgc aggcccgcgc
     6361 gcacggtagg ccaggtgtcc agcgccttgg gatggtcgat gtcaatgtcc tgggccaggt
     6421 cggggctgcg cgccgcctgc tgcaggcaca cgccataaag cgccatcacc gccagcacga
     6481 tggcataggg agcggcaccg cccagcatgg ctttcatgcc ttcgaacagg tagtacaggg
     6541 acccgatcac gatgatgctg cccgagatgc cgaaagcatt gcgcagcacg cgatcgcgca
     6601 gcggcttggg ttcgcgccgg atgatgggct gcatgcccat cttcagcgct tccaggtgca
     6661 cgatgtagaa caagccgagg taggacagga tggccggcag gaaggcgtgg cgcacgatgt
     6721 cggtataggg aatgccgacg tattccacca tcaggaaggc agctgccccc atgaccggcg
     6781 gcatgatctg accgtccacc gacgacatgg tctcgatggc gccggccttg accccgccgt
     6841 agccggattt cttcatcagc gggatggtga aaattccgcc agagaccaca ttggacaccg
     6901 acgacccgga gatcatgccg ttgaggg
//
LOCUS       DAIDFG010000166         6919 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_166_length_6919_cov_16.820804, whole genome shotgun sequence.
ACCESSION   DAIDFG010000166 DAIDFG010000000
VERSION     DAIDFG010000166.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 6919)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6919)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6919
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166_length_6919_cov_16.820804"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1109 a         2363 c         2200 g         1247 t
ORIGIN      
        1 agttccggtg ccaggtccgc cgcggtgatg gccacccggg cgtccgggtc ggtgatgtag
       61 tgcttgagct cctccgcgcg gttcatcggg ttgaccggca ccaccacggc gtcggcccgc
      121 aggatcgcaa agtgggcaat cacccattgc gggcagttct gcatgtccag caccacccgg
      181 tcgccttttt tgacgcccat ctcatgcagt gtggccgcca ggcgctcggc tttgtccagc
      241 acctcggcgt aggtgaagac acgtccgaaa aacaccagtg cagccttgtt cgggtaacgc
      301 agcgcgctga ccgccaggtt gtgccacagc gaggtggccg gagccgagat gcggtgcggc
      361 aggcgcttgg gccagacctt gtaatgcagg tcatggggag gggctggatg agtcgctgcc
      421 ggtgcagcgg tggggtggtc ggacaagtcg gggctccagt ttcacttcag gcgggcctcc
      481 agcctacccc ggctacgctg tcattgcatc ggggaagctc cgtataaaaa gctgtcgcct
      541 cagcgaccga gcctgaccat gatgaagccg gagacctctc cccgaggggg ccgccagaaa
      601 agcacaacaa caaccacacg gaaacaccct gtcacgtctt cgctcccctc ctgccctcca
      661 atgacgtgtg gcagcccggc cgcgtcggcg aaacgatgtg accgtaccgt ccggtgcagg
      721 accgcgctca agcggcgcgc gtttgccgcc atccgggcac aatcagcgaa taaccagacg
      781 gcagtcactc tgtttttgat agcagtctgc gtccgtctgt attgggctaa ggccctgttt
      841 tattgattat ttccagcacc tgccccgtgt acgtcaaacg cccgggtgct gtctgcccac
      901 ctgcatccca tgccgcctgt tctttatctc ttcgccctgt gcaacctggt catcggcagt
      961 ggcgcgtttg tgctgggcgg cattctggtg ccgctgagcg agtcgctcca cgtcagcctg
     1021 gccgcagccg gccaggccat gacggcctat gcggtgtcca ccgcgctgct ggcgccggtc
     1081 ttgatcattg ccaccgcacg ctggccgcgc aaacgtgccg tgcagctggc gctggccctg
     1141 ttcgccgccg gctgcctggt gtgcgcgctg tcgggcaacc tcgtcatgct gctgctgggc
     1201 cgggtgctga tgggcgccgg agcgatgttc agcgccgcgg cttcagcgct ggctgtcagc
     1261 ctggtggcgc cggcactgcg cggccgggcc ctgtccatca cctttctggg catgagcatc
     1321 agctatgcgg tgggcgtgcc ggtcggtgcc tggctcggct ttgaatacgg ctggcgcgtg
     1381 ccggtctggc tctcggccgg cgccagcctg gcggctctgg tggcggccag ctggctgctg
     1441 cccgccaaca tggcttcggc cggtgccaat tttgccggct tcggcgcggc ggcgcggcaa
     1501 ccggccgtgc tgcgcacctg gctgcgcacc ctgctttatt tcgtggcgat tttcagcgtg
     1561 tttgcctaca tcggtccggt gctgcaggcg ctcaacacca tgagctcgac gcagctgtcc
     1621 atcacgctgg cggtgttcgg cctggccggg gtggccggca cgctgtcggg tggctgggcc
     1681 aacgaccgct tcggctcact accgaccatg cgcgttcagc tggccgtgct ggtgaccacc
     1741 atggccgtgc tgccgctcac gcgtggccat ctgtacgcca ccatggcgac actcgtcgtc
     1801 tggggcattg cgggtttcgg cgtgatggcg ccgcagcaaa gccgcctggc cggcatggca
     1861 ccggcccagg cgccgctgct gctgagcctg aacggctcca tgctgtatgt gggcacggca
     1921 ctgggcgccg tcatcagcgg cgccatgctg gaccaccttc acctgtcgca actcggctgg
     1981 gtcggcgtgc ccttcggcct gctggcgttg ctgacgctgg tttttgaacc cgcgcatgtg
     2041 cggcccgtgg ccgacaccac tgcacactcc gcctgaaaga acacgccatg accacaccct
     2101 cttccgcact atccctgatc tacgtcggcg acccgatgtg ttcctggtgt tacggctttg
     2161 gcgtgccgct ggggcagttg ctcgaacgtt tccccgagct gccggtgcaa ctggtgctgg
     2221 gcggcctgcg cgcctacaac acgcaggtga tggacgccgc gctgaaagac aagctgcgca
     2281 acgcctgggc agaagtcggc aggcgctccg gccagccgtt ttcacaggcg ctgttcgagc
     2341 gcgaggattt catctacgac accgagccgg cctgccgcgc ggtcgtcacg gtgcgccagc
     2401 atgcgccggc gatggccctg cacatgtacc acgcgatcca gcaggccttt tatgccgaag
     2461 gacgcgacgt caccaaggca gcggtgctcg gtgaaatctg ggacgaggtg catggccgca
     2521 gcaaggaagc ctggggccac attgattttt tgcgcgactt cgagagtgaa gccatgcgcc
     2581 agcagacaag agacgatttt gcgcaggcgc agcgctgggg catacgcggc tttccggcat
     2641 tgctggccgt gcgtgatggc caggcccagt tgctggccag cggctacacc gaggccgatg
     2701 tgctcatcga gcgactggca ccactggcca acccgccgga cgacacaccc tagacatggc
     2761 gctttttcgg cgccggttcc gccgggaccg aggcctgcgc atcggccagc tcgtgcagcg
     2821 cttcgatgat gtggcagtgg gcgtcggttc cgtcgcagcg gttgcgcagc gccttgaggt
     2881 ctttttccag cgccttcaat tccgccagcc gcgcgcgcac atggcccagg tgcccgtcca
     2941 gcgcgccgcg tgcggccgcg caatcggtct tgttgttcag gtccagcccc agcagggtac
     3001 gcacctcggc cagcgacatg tccatggccc ggcacaggcg gataaaacgc agctgatgca
     3061 cgtcgccttc gctatacaaa cgatagctgt tgtcgccccg tccgagcggg ctgagcaggc
     3121 tttccttctc ataaaaccgg atgttcgcgg ggctgacgcc gctttgccgc gcggcttcac
     3181 cgatctggta acccctgggc gcggagggac ttgatacagg catcgcttga ccttgaagtg
     3241 acttgagggt ttccaatcat gccatcaacc cgatgtttct ggagaacccc tcgatgtctg
     3301 cccactgctg cgaccacgac gccaccgccg cccaagccaa cagcccgcgc taccggaaaa
     3361 ttctctggat cgcgctgctc atcaacttcg ccatgtttgc gctggaaatt gcggcgggct
     3421 ttcgctccgg ctcggtgtcg ctgctggccg atgccatcga ctttttcggc gatgccgcca
     3481 actacggcgt gagcctggcg gtgttgtccc tgggcctggt ctggcgtgct cgcgcggcgc
     3541 tgctgaaggc ttccagcatg ctggccttcg gcgtgtttgt gctggtgcgg gcatggtggg
     3601 cggccagcca gggcggtacg cccgaggcct tgaccatggg cgcgatcggc ctgcttgccc
     3661 tgatcgccaa tgtgtcggtc gctgccctgt tgtatgcgta ccgggaaggg gatgccaaca
     3721 tgcgaagcgt ctggctgtgc tcgcgcaacg acgccatcgg caacctggcc gtgatgctgg
     3781 ccgcgctcgg cgtgttcggg acccagagcg cctggcccga cctggcggtg gcggcggtca
     3841 tggccagcct ggccatctgg ggcggctggt cggtgatgca gcaggcgcgg cgcgagttgc
     3901 aggccggccg cttgtcccaa aagccggtat gaacaacctc acgcccgcgc agcgccgccg
     3961 gcttttcatg gcgctgatgc gcagtttcag cacggccgac tctgcagacg tcgctgatcg
     4021 ctgggcctgg ctcgaagccg cgcatgtggt cgggcagcag caattcgccc tgcactgggc
     4081 ctcgcatacg gcgatgctgc gcttcgcgct gcaaacccgg gacttgggcg aggcagcggg
     4141 gcaggtgcag cgcctggccc tggtcccgct gggccacctc ctgcggcgcc tgcccgccgg
     4201 caacagcgac cgcgccaccg tcagcgcgtt ggcgcccatg ccggttgatg cgcgtttgag
     4261 ccggctgatc cggcaggctg ccgagatggc cgaatcgcgt tgcttttgaa ctttccgcct
     4321 gccgccgtat catctaccta tgcgccgtcc cttgttcatt gccatgatct tcctgctgct
     4381 cctgcgcggc tgggtgggcg atgccatggc cactggcatg gccgccggcc atctgcaaca
     4441 agtttctccc gctacagaat cagtagctgc gcacgcccat gaatcatggg ctgacgacca
     4501 ttttgaccat gacatggcaa tggagccggc agcatccatg acgcattcgg cgactcctcc
     4561 ggattgcgca ggacacgcct ccggccagac ttccgacccc atgggcgcca ccgggcactg
     4621 cgactcctgc gccgcctgcc aggcctgcaa cacggtggcg ctgtcgccgg tgacagccag
     4681 cactgcctcc cttgccgttt cccctgcact gccgcactcg ccggccgcgc aatttgccag
     4741 cgccttcgcg acgctgggcc aaaaaccacc catttcctga cctccaggcc cgtagtgggt
     4801 ctgtacttgt catccggcac gggcagcctg ccctttttgc cggattcgcg cgatttttcc
     4861 tatcgccccg cttgtccctc aggagatttt tgtgaccttc cgtatccttt tttgcatgct
     4921 gctcctgccg ctgggcagcg tccatgcgca gggcgcaccg gccccgcagg ccgccgaccc
     4981 cgcggcgccc gtgccgacgg tgagctaccg ctccgtgttt gccgacctgc ccaccggcgt
     5041 ggaagctggc cgggaggact ggaaaaaagc caacaacgac gtcggccgct tcacgcgcgg
     5101 ccatatcgac atcctcaagt cggagcagca gcaggcagcg ccagcggccc ctgcggccac
     5161 gccggatacc gccccacctg cggcggccca tccctacaaa cattgagctt caccatgctt
     5221 tcattccaga aatccgcaac ggccctgctg gcggtcgcga cactggccgg ctgtgccagc
     5281 ctgtcgcccg acggcaacca gacggacatc aacaccctga ccgccggcaa gacagcgggc
     5341 atcaacgcgc cggtcgggcg cggcgacagc gcagaaaccc gcgacgcggt gacccggctg
     5401 ctggccgggc cgctgaccac cgaatcggcc gtgcgtattg cgctgctgaa caacccgggc
     5461 atccatgctt ccatggccac gctgaacatc agcgatgccg accgtgtgcg cgccggcacg
     5521 ctgccgaacc cgcatttctc gattggccgt ttcgtcgaag gcagcaaggt ggagatcgag
     5581 cgcggcatcg gcttcaatgt catcggcctg ctcaccctgc cctggcgcgc caaataccag
     5641 aaccagcagc atgaggtggc caaactgcag gcggcgcagg aggtcgtcaa actcgcatct
     5701 gacacgcgca aagcctggat caacgccgtc gccgcccaac agaccgccag ctacatgaaa
     5761 gatgccaagg aagcagccga ggccagcggt gaactggccc ggcgcctggc acgtgtgggc
     5821 aactggagca agctgaacca ggcgcgcgag caggcctttg ttgccgatgc cgcagcgcag
     5881 ctggcacgca gccagaacgc agcttatgcc gcacgcgaaa aactcatacg cctgatgggc
     5941 ctgtggggtg cacagacgca gttcacgctc agcgagcgct tgccggatct tccgaaaacc
     6001 attgccgaca tgccggacat cgaggctaga gccctgcgcg atcgcctcga cgtgcgcgcc
     6061 agtgtggcgc aggcaggcta cgtcgccgac aacatgggct tgacccgcag cacaggcttc
     6121 atcaacgccc tcgatgccaa atactcacgc aacaccacct ttgacaacgc caccggcgac
     6181 aaggagatca agcgcggctg ggagctggag ttgccactgc cgatttttga ctggggcggc
     6241 gccagcaacg cacgcgcgca gggccagtac atgcagtcgg tcgcgcgtgt gcgcgagacg
     6301 gccatccagg cccgctccga ggcgcgcgaa agttaccacg cctggcgcac cgcgcatgac
     6361 ctggcgctgc attaccgcaa cgaggtcgtg ccgctgcgca agttcatcaa caacgagctg
     6421 ctgctgcgct acaacggcat gctctccagc gtgttcgagc tgctggccga tacgcgcacc
     6481 agcatcacgg cggtcaacgc cagcatcgag gcgcagcgcg acttctggct ggccgatacc
     6541 gacctgcaaa ccacactgac agggacctcc gccggcatgg cgcccctggc cgccggcgct
     6601 gctgccggca gcagcgacgc caaaggacat tgacatgaac atcaacaacg aacgcagacg
     6661 attttttgcc ggcgcagccg ctgccacagc gactgcggtg gccgccagcg cggtcagccg
     6721 cgttgccatg gctgccctgc ccgagccggt gctgcagggc aaacccgaca ccatgccgcc
     6781 gctggtcccc agcaccggcc ggccctacaa cccggtggtc acgctcaacg gctggacgct
     6841 gccctggcgc atgaatagcg gcgtcaagga gtttcacctg gtggccgagc cggtggtgcg
     6901 cgagatggcg cccggtttc
//
LOCUS       DAIDFG010000167         6913 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_167_length_6913_cov_20.724264, whole genome shotgun sequence.
ACCESSION   DAIDFG010000167 DAIDFG010000000
VERSION     DAIDFG010000167.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 6913)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6913)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6913
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167_length_6913_cov_20.724264"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1156 a         2103 c         2307 g         1347 t
ORIGIN      
        1 ttcttcgagg gggacataag cctcggattt gagtccagca ttcacaacca cgtggttgtg
       61 atcaatacgc actacctcgg cggtaatgac ttcgcccgta cgcatttcgg agcgtttcag
      121 cgattcttca aagagggcgg caaaagattc agacattgat tttcctagtc cacagagcag
      181 gtttccgacg gcaccattgc tatcgttttt agagctgact gcggcggttg tgtgcgggtg
      241 accgcggtta agttagtgat cctgccgacc gaggcactgg cgtgcctaca aaaatgtatg
      301 agggtcagaa gggccgggtg ccttgccaga gttgcaacac ccggtctacc gattcttcaa
      361 tcgacagctc ggagttgtcc agtagcacag cgtcatcagc aggcttcaaa ggggcggatt
      421 gccgtgacat gtcacgcgcg tcccttgcct ccaaatcctg ctcaatcgcg gctattgtag
      481 ccggaattcc ctttgaaatc aattgcttat ggcgtcgttc cgcccggtgc cgggcgctcg
      541 ccgtcaggaa gattttcagc gcggcgtccg ggaaaatcac ggtgcccatg tcccgcccat
      601 cggccaccag ccccggcaac tgccgaaagc cccgctgcaa ctcggtcagg gcgtcgcgca
      661 cggcgggatg caccgacacc cgggaggcgg ccatgccggc ggcttcgctg cggatggcgt
      721 ccgacacgtc tttctggccc agaaacacct gttcgccctg aaaacgcacc ggcagggtgc
      781 gcgcgacggc ggcgacgccg gcttcgtcat cgagcgccac gccggcacgg ccggcggcaa
      841 acgcggtcag gcggtacagg gcgccggaat cgaggtagtg gtagccgaga cggtgcgcca
      901 ccagggcggc cagcgtgcct ttgcccgacg cggtggggcc atcgacacag atcaccggga
      961 tcagccggcg ctcggtcacc gacaccgaaa acagggcttc gaagtagtcc ggaaaggtct
     1021 tggcgacaca tttcgggtcc agaatgcgca ccggcagacg cgccgggttg aaggccgcca
     1081 gcgaaaaaca catggccacg cggtgatcgt cgtaggtgtg gatgggggcg gtgcgccact
     1141 ggattggcgg cgtgatgcgg atgtagtcgg cgccttcctc cacgccggcc cccagcttgc
     1201 gcaactcgca ggccatggcg gcgatgcggt cggtttcctt gacgcgccaa ctggcgatgt
     1261 tgctcagcgt ggtggtgccg tccgcgtaca gcgccatcac ggccagcgtc attgccgcat
     1321 cggggatgtg gttgcagtcc agggtgatgg ccttgagcgg ccaggcgccg cgggagatac
     1381 gcagggtatt gggtgtgctg gctatgtgtg cgcccatcat ccgcgcggct tccacaaaac
     1441 ggatgtcgcc ctggatggag tccgcaccca cccccacaat ttcgatgcca ttttggcctg
     1501 aagccaccgc tgctattgcg ccaagcgcta tgaaatagct agcggatgaa gcgtcccctt
     1561 cgacatggat ctcgccgggc gaccggtagc ggctgccggc cggaatggtg aaacgccgcc
     1621 agctctcgcg ctggacggcg atgccgaaac gcgccagcag gttcagcgtg atctcgatgt
     1681 aggggcgaga gatcagctcg ccgaccacgt cgatgtgaat agcgcgtgtg gccaccagcg
     1741 gcagggccag cagcagggcg gtcaggaact ggctggagac gtcgccgcgc acgcggatgg
     1801 gcgcatccag tgagagctgg ccggtacgga tacgcagcgg cggaaaaccg ttttcgccca
     1861 gatagtcaat ggcgcaaccc aggccgcgca gggcatcgac caggtcgcca atcggacgct
     1921 catgcatgcg cggcacgccc gacagttcaa agtctccacc cagcagcgcc agcgccgccg
     1981 tcagcggtcg catggcggtg cccgcgttgc cgagatagag cttggcctgc ctttgcgcca
     2041 gctggccgcc caggccgtcg atgacggcgc tgttgccgct ttgtgccacc gtgcagccga
     2101 gctggcgcag cgcggccagc atcacggcag tgtcgtcgga agcgagcagg tcatgcaccg
     2161 tggtgcggcc ttcgctcagc gccgcgagca gcaggacccg gttggaaatg cttttggagc
     2221 cgggcaggcg cacgctaccg ccggcacccg tcaatgctgg aatgtccagg aactcggtat
     2281 cgaacatggc cccgcgccta cttgagggct ttgggggggg tgatccgcca gttggcgcgg
     2341 gtatggctgg catggtcgat acctgcttcc agtgcatcgc cgtcgccgct gctgatcagc
     2401 ttttcaagcg aatgcagggt ggcctggaaa atcttcgact gctccagcaa ctcctcccgg
     2461 ttggcaatca ggatgtcccg ccacattttg gggtcgctcg ccgcaatgcg ggtgaagtcg
     2521 cgaaaccccg ggccggccag cgacaggtag tccttgccct gggcctggcc ggagatgctg
     2581 ttgatcagtg caaacgcgat caggtgcggc agatggctga cggcggcata cgcggcgtca
     2641 tgcgactggg gcgacatctt gacgacgttg cagcccagcg ccgtccagac atccacggct
     2701 ttctgaagct gaaccgtgaa ggtgcgttcg atcggcgtca ggatgacctg ctttccggtg
     2761 tacaggtccg catcggcatg ttcgacgcca gaaacttcct tgccggtgat cggatgggcc
     2821 ggcacaaaac agccgaggtg gtcgcgcagc acccggcgcg cggcgtccac gacatcgcgc
     2881 ttggtggagc cgacgtccat gatcagcgtt gtcggggtca cgagatggcg gatggccttg
     2941 aaggtggcct cggtggcgga cacaggcacg gccagcagca cgatgtcagc accggagacc
     3001 gccagcaatg ccgaaggcgc ttccacgtcg atcacgccca tctggcgcgc gcgctcggtc
     3061 gtggacggcg acttgctgta acccaccacc cgtttgacca gacctgcgcg tttgagcgcc
     3121 agcgcaaagg agccacccat caggccgcag cctatcaatc ccagttgttc aaacatgtcg
     3181 gggggccgtg gttacttgca attgcgtcat ggtttcagtc gctgaccgga taggtgccca
     3241 gcactttgta gaacgcacag agctgctgca ggtctttcag ggccgctgcc acgttgggtt
     3301 gcgaagggtg accctgcaag tcgatgtaga aaaaatactc ccactggccc gagcgggcag
     3361 ggcgcgattc gaagcgtgtc atcgacacac catgggtttt caggggaacc aggatgtcgt
     3421 gcacagcacc tggcttgttc ggaaccgaca cgatcaggct gacgcagtcc ttgcccgacg
     3481 cctgcggcgc gcccagcacc tgcggcaggc agatcacggc aaagcgggtg cggttgaacg
     3541 cgtcgtcctg gatcgcgtga gccgcaatgt gcaggccgaa ttcggcaccc gcccgttcgc
     3601 tggcgatacc ggcccacgcg gggttgctgg ccgccaggcg ggcgccttcg gcattgctgg
     3661 agaccgcgcg ccgctcggca tggggcagat gcgtggcgag ccagccctgg cattgcgcca
     3721 gtgcctgtgc gtgggccagc acgacctcga tgccgtctaa cgacgccgtc aggcgcagca
     3781 ggttgtggcg caccagcagg ctggtttcgc ccaccacatg caggggtgaa ttcaggaaca
     3841 ggtccagcgt gcgtgtcacc acgccttcgg tgttgttttc cacaggcacc accccgaact
     3901 gggcggtgcc ggccgtcgcg gcgcgaaaca cctcgtcaaa actggcgcag ggcacgtgtt
     3961 cgatgctgga gccgaaaaac agcacggctg cctgctcgct gaaggtgcca gcagggccga
     4021 ggtaggccac gcgctggggt gcttcgagcg cgcggcaggc cgacatgact tcgcgccaga
     4081 tgtgggcgat gttgtcccct ttgagcgggc cgggattggc cgcctgcagc ccctggatga
     4141 cctgggcctc acgttccggc ctgaagaccg gcgatccgtc ctggcgtttg atctcgccga
     4201 cctcgttggc cagcgaggca cgctggttca ggagcgccag cagttgctgg tcgacagaat
     4261 cgatttttac gcgaagttgc tgaagacttt gggtcatgga taaggcgtct caggcccggc
     4321 gggcaggagg gcgtcgcaca ggtggaaggt tgggcgacgg cgcgccggct tggggaaaca
     4381 gccccgcgtc atttcttcgc gggtttggcc gcgggggcgg cgcctgcagg cttcgctgcg
     4441 ggtgcggcgc cgacgatttt ggtggcggtt tcgttgaact gcttggcgcg ggcctggatc
     4501 tcggttctgg aggactccac cagttgtttc acgaagacat tgaccagatc gggcgtggtt
     4561 gccttgtatt tctttacggc aggggagtcg aagaaagcgg tgagttgctt gagttcttcc
     4621 agggtaaagc ggtccatgta ggccggtacc agggcatcct tgttggcctg actgaccttg
     4681 ctgccgatga tcttgttggc atctgcggcg aacttggcaa gctcggcgtt gaggtcctcg
     4741 gtggccttgg cctgcttggc tttgggaacg gaggtttcca gcctggattc ccagccggag
     4801 atcaggtcct gggtggcgct gccggccagt tgttcgacca ggctgtccag gtcggggccc
     4861 tgctgcaggg cgacgagccg ggtggcccac tcgagcttgg ggtcggtcgt ctgggcctga
     4921 ctcgcggccg agaaggcgca gccagcaatc aggagggtgg tgagcagctt tttcatcaat
     4981 tcgtttccgg gttggaggca ggtgcgcctg tgtcagtgtc ggtttcattg tcgtccaggc
     5041 tggcgtcgtt ttccacgatg cgctgcaggc cgctgagctg ggagccttcg tcgaggccga
     5101 tcagcgtgac gccctgggtg gcgcggccca tctcccggat ttcgctgaca cgggtacgaa
     5161 ccagcacacc cttgtcggtg atcagcatga tttcgtcgtc cacgtgcacc agggtggctg
     5221 cgaccacctt gccattgcgc tcgctttgct ggatggcaat catgcctttg gtgccacggc
     5281 cgtggcgcgt gtattcctgg atggaggtgc gtttgccgta gccgtgctgg gtggccgtca
     5341 gaacgctttg ttcttcatct tccgcgacca gcatggcgat cacgccctgg ccgtcgtcga
     5401 gcgtcatgcc gcgcacgccg cgggcattgc ggcccatggg acgcacgtcg ttttcatcga
     5461 aacgcaccgc cttgccaccg tccgagaaca gcatcacatc gtgcttgcca tcggtgagtg
     5521 cggcgcctat caggtagtcg ccgtcgtcga ggtccacggc aatgatgccg gccttgcgcg
     5581 ggttgctgaa gtcttcgagc ggggttttct tgaccgtgcc catgctggtg gccatgaaca
     5641 catactggtc cgcagggaag ctgcgttttt cgccggtcag cggcagcacc accgtgattt
     5701 tttcgccttc ctgcagcggg aacatgttga cgatgggacg gccacgcgag ccacgcgagc
     5761 cagccggcac ttcccacacc ttgagccagt aaagccggcc gcggttggaa aaacacagga
     5821 tgtagtcgtg cgtgttggcg atgaagagct ggtcgaccca gtcgtcttcc ttggtggccg
     5881 tggcgagctt gccgcggccg ccgcgctttt gcgcccggta ttcggaaagc ggctggctct
     5941 tgatgtagcc ggtgtgcgag agcgtcacca ccatgtccgt cggcgtgatc aggtcttcgg
     6001 tgctcaggtc aaacgaactg tgctcgacct ggctgcggcg caggcccagc ttggactggc
     6061 cgaactcctg gcggatgaca gcgagttctt cgccgatgat ggtcgagaca cgttccggac
     6121 gcgccaggat gtcgagcaga tcgtcgattt ccgccatgac ctccttgtac tcggcaacga
     6181 tcttgtcctg ctccaggccg gtcaggcgct gcaggcgcat ctgcaggatt tcctgggcct
     6241 gggtttcgga caggcggtac aggccgtcgc tgcccatgcc gaagtccttc tccagcccgt
     6301 ccgggcgata gtcgtcggcg ttgaccacgc cgccgtccgc gcgggtgcgt gtcagcatct
     6361 cgcgtaccag gctgctgtcc cagggacgca gcatcagctc ggccttggcg accggcggtg
     6421 tcggcgcgtt gcggatgatc gcgatgaaat cgtcgatgtt ggccagcgcg acggccaggc
     6481 cctcgagcac atggccgcgc tcgcgcgcct tgcgcagcgt gaacaccgtg cgccgcgtga
     6541 ccacctcgcg gcggtgcgac aggaacacct ggatcaggtc tttcaggttg cacagcttgg
     6601 gttggccgtt gaccagcgcc accatgttga tgccgaaggt gtcctgcagc tgcgtctgct
     6661 tgtacaggtt gttcagcacc acctcgggca cttcgccgcg cttgagctcg atgaccaggc
     6721 gcatgccgga cttgtcgctt tcgtcctgga tgtggctgat gccctcgatt tttttctcgt
     6781 ggaccagctc ggccatgcgt tcctgcagcg tcttcttgtt gacctggtag ggaagctcgt
     6841 caacgatgat ggactggcgc tggcctttgt cgatgtcctc gaagtggcag cgcgcacgca
     6901 tgacgacctt gcc
//
LOCUS       DAIDFG010000168         6857 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_168_length_6857_cov_20.124669, whole genome shotgun sequence.
ACCESSION   DAIDFG010000168 DAIDFG010000000
VERSION     DAIDFG010000168.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6857)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168_length_6857_cov_20.124669"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1189 a         2218 c         2173 g         1277 t
ORIGIN      
        1 ccgaccgtgg cgtccatcag gaagttgatc ttgtcgaaga ggaaagccgt gtgcgagtcc
       61 agcgagtcga tatcgcgcag aatctggcgc gcctcctcga actgttcgct gttgagcatc
      121 ttgctgcgca tcatgaaact cacggcgcgc cgcgtgtcca tcacgttgcg gcggatgcgt
      181 ccgctcaggt cttcgtgccg ggcgatggcg cccagcacct cgccggccat cacgtccgtc
      241 acatcgcccg acagcacctt ggtgctgacc ttttcaaggt cgatgtaaat gccttcgagg
      301 gtgtcggccg agtattcggc atcggcgtcg aacagcttga gcagcacttc cttggcgtct
      361 tcgatcaggc ccggcgcccg gcgcgcgcgc atgcgcagca ggcggaagac cggcacgtct
      421 tcgtcgtgga tcgaaaacag cacgcccttg ctcttgaggt tgtcattgac caggttcagg
      481 atgaaggcta cccgcacggt gcggggttcc tcgtcgtcgg caatcagaaa gtccgagcgg
      541 atgtgcagct cgccgttgtc ttcttcgtaa aagcgcgcgg actcctcgat gtcctcgtcc
      601 atcacgtctt cgggaatcga caggccgtaa tactgcttga tccagcgttt ttcttccagc
      661 gtcggcgact ccaggtccac ccagatcggc tggaaccggg acagttcctc cagcgattcg
      721 atctcttcct ggaacaggcg cccgttggcg agcgtgaaaa tattgagcat gactggctcc
      781 caatcggttt gggggcgccg gcaccgggca ggcgtctgcc cggcgttggc ggggttgtgg
      841 atggtggcat cgctttcaac cccgggatga cgctggcgtc agtctgcggt gctgaaagct
      901 agcgactggg gctgctttcc aaggatttct ccgtattgac caagctgcga ttatggcatt
      961 ttggcccgga tttcactacc tgttccggcc cgccgccggg ttcccgccgg ggggtcaagg
     1021 accttttccg accgtttcat tttttgttgc gatgcacggt tgcgtttgcc ccggttcggg
     1081 cgcatagtga aaacaagaga gttgtccgtc cgccgcccgg caggtcttgc caaagcggtg
     1141 tttttaaccc ccctccagtc atggaaaagg agtctcttta tgaatttgac gatcagcggt
     1201 caccaccttg aagttacccc cgccttgcgc ggttacgtga ccagcaagct ggatcggatt
     1261 acccggcatt ttgaccaggt ggtggatgtc aaggttctgt tgacggtgga taacatgaag
     1321 gaaaaggagt tgcgccagag agccgagtgc aatgtgcatg tcaagggacg tgacctgttc
     1381 gccgaaagtt cgcatgccga cctgtatgca gcggtggatg aactcgctga caagctggac
     1441 cgccaggtcg gccgctacaa gaccaaaacc caggatcacc accacgtgac tgccaagcgg
     1501 gagatgtaag cgccgccaca ggcggcgggc tggcgcaaaa tgcccgcccc ctcccggaag
     1561 gccgcctgtg ccctcaggcg gcttttttat ggcccgaatg gccgccatgc gtgtgaaagt
     1621 gcataattcg gcgaccccac cttgttatga accgtctttc gcaaatattg ccccctaccc
     1681 aggttctggt cagtgtcgag gccaccagca agaagcgtgc ttttgaagaa gccggtctgc
     1741 tgtttgagaa cctgcatggc ctgaaccgcg cgctgatcac cgacagcctg ttcgcgcgtg
     1801 agcggctcgg ctcgaccggg ctgggtcatg gcgtggcaat tccgcacgga cgtatcaagg
     1861 gcctgaaagc cccgatggcg gctgtgttcc agctgcacgc tccgatcggc tttgatgcgc
     1921 ctgacgagca ggccgtgggc ctgttgattt ttctgctggt gcccgaagcg gccacccaga
     1981 aacaccttga aatcctgtcc gaaatcgctg aactgctcag cgacagcgcc ctgcgcgagc
     2041 ggctcaaggc cagcaccgac gcggctgtgc tgcatcagct gattgcctcc tggcaatcca
     2101 gccacgctgt cagcccctga cccgcgcgcg tgaccgtgac gcagattttc tccttcccag
     2161 cggcatgaag cccaccgtcg tcagcgccga cgtcctgttt gaggaccacc gcgccgcgct
     2221 gaagtgggag tgggtggccg gcctgggcgc ttcggagcga cgcttcgacg aggtcgccgt
     2281 gcgtgcggcc cgctccggcg ccgacctggt cggttacctc aactacatcc acccctaccg
     2341 cgtgcagctg ctgggtgagc gcgaagtcgc ttacctgagc aacggcagcc cggaggattg
     2401 cgcccgccgc atttcgcgca tcgtcacgct ggagccgccg gtgctgatca ttgccgacgg
     2461 ccaggtggcg cccgacacgc tgctgtccat gtgcgagcgg gcccagatac ccatgtttgc
     2521 cacgaaggaa tcggctgcgt tcgtgatcga cgtgctgcgg gcctacctgt ccaaacattt
     2581 cgccgaccgc acctccatgc acggcgtgtt catggacatc ctcggtgtgg gcctgatgat
     2641 caccggcgaa tcgggcctgg gcaaaagcga gctgggcctg gagctgatct cgcgtgggca
     2701 cggcctggtg gccgacgacg cggtggacct gtaccgcatc aaccagacca ccatcgaggg
     2761 ccgctgcccg gaactgctgc agaacctgct cgaggtgcgc ggcatcggcc tgctcgacat
     2821 caaggcgatt tttggcgaga cggcggtgcg gcgcaagatg cggctcaagc tgatcgtgca
     2881 cctggtgcgg cgcgaaacgc tggagcgcga gtacgagcgt atcccctatg agccgctgac
     2941 ccaggacgtg ctgggcattc cggtgcgcaa ggtgatcatc caggtggtgg ccgggcgcaa
     3001 catcgcggtg ctggtcgagg ctgcggtgcg caatgtcatc ctgcagctgc gcggcatcga
     3061 cacctaccag gaatttgtcg aacgccatcg ccaggccatg gagcggggcg gctgaatagc
     3121 ccccacggtc gctcacttcg tgtagctccc gaggccttgc aggccgcggc tcgcgggacg
     3181 cttcgcctct gtcgcctgtc caaagctgcg caggcagctt tggagccgca ggctccagcc
     3241 cctcaggggc cgctgcgcct gcggcccggc gaagccggtt ccgcggcccc tgcttgaatt
     3301 gagagcacct tcgctcgccc acgtcgtgag cggcccggtg cgaaaccctt acctcatatt
     3361 ttgctattga attaatagct gtttacgccc accaatcgag ggctaccgcc caattccatc
     3421 aaaaatcagc cgtggtgcgg atgccgtggc gacacgggac ggttcgggca cttttccttg
     3481 gtgcagttgg catacatcga cagcgagtgg tcggcgatcg caaacccctt ggccttggcg
     3541 accgcgtgct ggcgcttttc gatttccgcg tcgtagaact cctcgacctt gccgcaatcg
     3601 aggcacacca ggtggtcatg gtgctggccc tcgttgattt catagacggc cttgccggat
     3661 tcgaaattgc tgcggttgag caggccggcc tgctcaaact gggtcaacac gcggtacacc
     3721 gtggccagcc cgatgtcgga gcggtcttcc agcagcaccc ggaaaacatc ttccgccgtc
     3781 atgtggcgct gcttgccggc ctggaaaatc tccaggatct tcaggcgcgg cagggtggct
     3841 ttcagtccgg tgttcttgag ttcgtcgatg ttggtcatgg gggaggcttc ctgaggaaaa
     3901 ggggccgcag tcggggcctg tgcaggctcg gcgttcaggg ggcgggaggc gcgtacgcgg
     3961 cggcaccctg ccgctacaat ggccaatcat atcgctacac ccccctacta tgtcagccat
     4021 gcgtgcaaat taccgtcgcg gtctccaagc cgctttcgcc gtcctggcct gcgccggact
     4081 ggctgcctgc ggcagcggca aaggcatgac cgggggcttg agcgaaagca aaatcaatcc
     4141 cgtcaactgg atcacgccct acaaggtcga gattgtccag ggcaacttcg tctccaagga
     4201 gcaggtcgaa ctgctcaagc ccggcatgac ccgggaccag gtgcgccagg tgctgggcac
     4261 gccgctgctg gcgagcgtgt tccatgcgga tcgatgggac tatgtgttta ccctcaagcg
     4321 ccagggcgtg gagccgcagg cgctcaggct cacggttttc ttcaagggtg atgccctcga
     4381 gcggtttgag ggcgacgcca tgcccagcga atcggacttc gttgccaagc tggactcgcg
     4441 ccgcaagctc ggcaaggtgc ctgtgctgga gctgaccgaa gagcagctcc aggccgctga
     4501 aaagaaaccg gccgcgacgg ccgccaccac cccggtgacg ccccccgttt ccgcgccgtc
     4561 ggccgccagc tatcccccgc tggaaagccc gacccggtga gcgcgttgca gcaggcccct
     4621 cttcgcatcg cggtggccgg cgccagcggc cgcatgggcc agatgctggt cgaggccgtg
     4681 cgcaccagcg gcgactgcca gctgaccggc gcgctggatg tggccaccag cccagggctg
     4741 ggcagtgatg ccgcggcctt cctcggacag gccagcggag ttttgatcac cgccgacctg
     4801 gccgccggct tgggcggctc ccaggtgctg atcgacttca cccgcccgga aggcacgatg
     4861 gcccatttgg cccaatgccg cgaactcggc gtcaagctgg tgatcggcac gaccgggttt
     4921 tccgacgcgc agaaggccga gatcgccgct gccgccaaag acatcgccat catgatggcg
     4981 cccaacatga gcgtcggtgt gaacgtcacg ctcaagctgc tcgagatggc cgccagggcc
     5041 ctggacaccg gctacgacat cgaaatcatc gaggctcacc accgccacaa ggtggatgcg
     5101 ccgtccggca ccgcgctgaa aatgggcgag gtgatcgccg gcgcgctcgg gcgcgacctc
     5161 aaggactgcg cggtttacgc ccgcgaaggc gtgaccggcg agcgtgatcc ctcgtccatc
     5221 ggttttgcga cgattcgcgg cggcgatatc gtcggcgacc ataccgtgct gtttgccggc
     5281 accggcgaac gcatcgaaat cacccacaaa tcctccagcc gcgtcaccta cgcccagggc
     5341 agcctgcggg ccgcgcgttt tctggccggc aaaccggccg gcctgtacga catgttcgat
     5401 gtgctgaacc tgaagtgagt gcctgaatga acgtgctcgc gttgatgatt cagggcgacg
     5461 ccgtgaccgg cctggtggcg gcgctgctgc tgctgatgtc ggtggccagc tgggtcgtga
     5521 ttgtctggaa agcctggctg ctcaagcggg cggtcggcga tgtggcgcgc agcacggccg
     5581 tgttctggca atccgcctct gtcgaggagg cccagcccaa ggtggctgcg ttcgaccgcg
     5641 aggcgctggt gctgcccctg atcgctgcca cccgactcca ggccggcggc accctggcct
     5701 cggccggaga caggtcccag cagctgacgc gggtcctgcg cgatgcgctg catgaggtgc
     5761 tgcacaagct gcagtacggc caggttctgc tggccaccgt cggttccacc gcgccttttg
     5821 tcggcctgct gggcaccgtc tggggcattt accacgcgct gaccggcatt gccgcggccg
     5881 gccagatcac catcgacaag gtgtccggtc cggtcggcga ggcgctgatc atgaccgccg
     5941 ccggcctggc cgtggcgatc ccggccgtgc tggcttacaa catcttcggc cgcaacatcg
     6001 gccgcatcga ggcggacctg gaagggtttg cctacgattt gcgggaattg ctggctgtag
     6061 cgggccccgg cggcaaggcg gcggaacgca gcaaagagtc agtggacgca ggcgcgaggg
     6121 cgtcgtgagc ttcggccgcc tcgaacgcac ccagggcgcc cagcccatga gcgagatcaa
     6181 cgtcaccccg ctggtggacg tgatgctggt cctggtggtg atcttcatca tcaccgcgcc
     6241 cctgctggcc agctcgatca agctggacct gcccaaaagt gatgccgccc ggccgaacga
     6301 ggcgccgcaa tttgtcacgc tggtggtcga cagggccggc caggccttcc tcaatgacca
     6361 gccggtctcc ggcgaccagc tggaccagcg cctgcggcaa aacgcggcgc tgaaccccga
     6421 cacggaagtg cagctgcgtg ccgacgaggc cgtgccttat ggccgcatcg tcgaggtcat
     6481 gggcatcgcg caaaaagccg gcctgaaccg catcggtttc gtcgcgaacg cccccgcccc
     6541 cgcaacgcca gcccccgcga cccggccctg aaggcgctcc agcggggcca gtccggtcca
     6601 gcgcctaaaa tccctgcaac cggcagccag gggctgcttc attttttttc catcttccgt
     6661 gcgccatgca agacaaatac aaccacctcg acgttgagcg cagtgcgcaa acccactgga
     6721 ccgccgcgga tgcctaccgc gtcaccgaag actcgtccaa aaagaagtat tacgcctgct
     6781 ccatgctgcc ttaccccagc ggcaagctgc acatgggcca tgtgcgcaac tacaccatca
     6841 acgacatgct cacgcgt
//
LOCUS       DAIDFG010000169         6838 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_169_length_6838_cov_18.942651, whole genome shotgun sequence.
ACCESSION   DAIDFG010000169 DAIDFG010000000
VERSION     DAIDFG010000169.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 6838)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6838)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6838
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169_length_6838_cov_18.942651"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1243 a         2075 c         2210 g         1310 t
ORIGIN      
        1 ggtttcggtc gccggcacgc gctgcacgcg gtggccgccc gactcgaaac gcagcttgcc
       61 gtaaacaccg acgcccaagg catcaggcgg gccgtccagg cgcagcacca cttccttgta
      121 gccgccgagc tcgctgaccg actcgctgat gatctcgacg gtccagcgct tgcgttcggc
      181 gtaacgcgtg tacatgcgaa acaggtcggc ggcgaacagc gcggattcat cgccgccggc
      241 gccggcccgg atctcgatga acgccgggcg cgcatcgtcc gggtccttgg ggatcagcat
      301 cagctgcagg gcttcgtcca gccgggcaat gtcggcctgc gcggccgtga tctcttcttc
      361 ggccatggcc gccatctcgg ggtcgtcttt cgcctcggcc agcatggcct gcgcactggc
      421 cagatcagcc tcgcgcgccg tcagttgctg gtactgcgtg accacgatgc tggcctcggc
      481 atgttcacgc gtcaggttgc gaaaacgctc catgttgctg accacgtcgg cggccgacag
      541 cagtgcgtca agctcgtgca gccggaaaat ctggcggtca agtgtctggc ggagaaaggg
      601 tttcattgaa aggccgggag caaaaacgtt caggagacag gcggccggta acccggcccc
      661 gcgttcagcc cgatgcacgg gctgtggttc gcgcgacagt gacgctaacg ctctttctgt
      721 tcccttggca ggttgccgcg caaaaacagc cgggagacgg tttgagccgt cgcttcgcgg
      781 gtgtccgcac tgccggcatg cagctcggcg agtgcgccgt gcagcatttt ctgcgtcagc
      841 ccgcgcgaca gcgcctcgag gaccgcatcc acaggctcgc ctttggccag cagttttttg
      901 gcacgggcga tttccagcgc acgccattcg tcggtctggg cgttgagctg ctggatcagc
      961 ggcacggtgc gccgctggtc cagccagtgc atgaagttct gcacaccggc gtcaatgatg
     1021 acttcggcct cggccactgc ggcctgccgg ctgtccttgc cggcctgcac cacatgcgcc
     1081 aagtcgtcga cggtgtagag gtagatgtcc ggcagttcct tgacttccgg ctcgatgtcg
     1141 cgcggcacgg ccagatcgac catgaacatc gggcggtgct tgcgcagctt gacggcgcgc
     1201 tcgaccgcgc ccaggccgat gatgggcagg gtgctggcgg tgcagctgat caccgcgtcg
     1261 aactcatgca gacggccagg caggtcggcc aggcgcatga cctcggcgcc gaagcgtccg
     1321 gcgagctttt cgccgcgctc caaggtgcgg ttggccaccg tcatggcttt cgggtttttg
     1381 gcggcaaagt gggtagcggc caggtcgatc atctcgcccg cgccgacaaa caggaccttg
     1441 atgtcgccca gatcctcgaa cagctggccg gccagccgga ccgacgcggc cgccaggctg
     1501 atgctgtgcg cgccgatctc ggtgcttgtg cggacttcct tggcgaccgc aaaggagcgc
     1561 tggaacagct ggtgcagcgt ggtgcccatg gcgccggcgt cttcggcggc gcgaaccgcg
     1621 tccttcatct ggcccaggat ctgcggctcg cccagcacca tggagtccag cccgctggcg
     1681 acgcgaaagg catggcgcgc ggcatggtcg ttttccaggg tgtaggcgtg ggagcgcagc
     1741 agggccggcg agacgccgcc gcggtgcgcc agccattcga gcgtgtgctc gaggtcggac
     1801 ttgtcgccgg cgcaatagat ctcggtgcgg ttgcaggtcg acagcagcgt ggcctcgggc
     1861 tggcgcgcca gggactcgcg caagcccctc aaagtgggct cgatctggtc gatggcgaac
     1921 gcaaaccggc cgcgcaaatc gagcggtgcg gtggtatggt tgatccccaa ggtccagact
     1981 gacatggccc gattataaaa ttgaagggat ggtccgacgc ggacattctt tcaatgccct
     2041 tgatacaggg caagccgggg ggcagatggc gcgcccggct cctttcgatg attcccgcct
     2101 gaatcccttc aatccgccac cggcatgact gcactcaacc tgatcaacca gctgctcaat
     2161 ttcatggctc cggccgcctt tgtggcgctg ttcgtggtgc tggcggcccg tgcctttgcc
     2221 cgaattctca gatcaaaaag tcctctgacc ctttccctgt ctgcgcagat agctattgtt
     2281 tttgtagcgt ccctgacctt gttggtggcc ggcctggtca tttttggccg cgacggcaag
     2341 atggccagtt atgccctgct ggccgtgggt gcggcgacct gccagtgggt gttgctgcgt
     2401 ggctggaaag cctgaacgcc ctgctcaggc gtcgggggcg aacaggtcga tccggtcggt
     2461 gatgatgccg tcggtcccca gatcgagcag gcgctgggct gcccattcgt cgttgacggt
     2521 gtaactcagg caacgaaaac cggcgctttt ggcctgcagc acggtggagc tgtcccacag
     2581 ggcctggttg cagaccagcg ctgcgcagtc cagcgtcagc gctgtttcca gccagccctt
     2641 ccacaaactg tcgagtaaca ggccgcgcgg cagctgcggt tgtgcggctt ttgcggcttc
     2701 cagcgcctcg ggctgaaaag aagtcagcag cggcggcacg gcggcacctt cccacaggcg
     2761 ggccgcggcg ttggccacga cggtgccggt atgcgcttcc gcgcccgggg tgggcttgat
     2821 ctcgatgttc agaaaatagc cgttgcgcag gcaataccgc gcgacgttgt ccaggctggc
     2881 cagcggctca ccggcataag cgcgcgagtg ccagccgccc gcgtcgagcc gcgacagcgc
     2941 ctcccaaggc tgctcgccgc cagtgccccg gccgctggtg gtgcgttcca gcgtggcgtc
     3001 atgcatcagg aagggcacgc cgtcggcact gagcttcaca tcacattcga acatgcggta
     3061 gccgtgggcc gcgcccaggc ggaaggctgc cagcgtgttt tcaggcgcca gcttgccggc
     3121 gccgcggtgc gcgatccagc gcggataggg ccaatcgggc aaaccggtca ccgcgtcaga
     3181 ggcgcttgcc cgtggcgccg tcaaaccagt gcacccggtc ttcgcgtggt gcaacctgga
     3241 tggtgctgtc gggcttggga gcggggtact gttcgtcggt gcgcacgatg agcgtctcgt
     3301 ccccaaggcg gcagtaaata aggcgctcgg cgcccagcag ttccacggtc tcgaccttca
     3361 gttcccagcc agtgttgcca atgtccagat gctcgggtcg tatgcccatg atgcttccct
     3421 cgcgtgcgcc gggggcgttc ttgagcagat tcatcggtgg tgaaccgatg aagctggcca
     3481 cgaaggtgga cgctgggcgg tgatacacct cttcgggtgt gccgaattgc tccatcacgc
     3541 cggcattcat caccatcatg cgctgggcca gcgtcatcgc ttcaacttgg tcgtgcgtca
     3601 cgaacagact ggtgatgccc agttcgcggt gcaatttctg aatctccagc cgggtctggg
     3661 cgcgcagctt ggcgtccagg ttggacagcg gctcgtcgaa gagaaaaacc tgcggctggc
     3721 gcacgatggc acggcccatg gccacacgtt ggcgctggcc acccgaaagt tgccgcggct
     3781 tgcggtccag tagatgaccc agctccagaa ttttggcggc tttgtcgacg cgggtcttga
     3841 tttcgtccag cggaaccttc ttgatcttca gtccataagc catgttgtcg aagacgctca
     3901 tgtgcgggta cagcgcgtag ttctggaaca ccatggcaat gtcgcgttcg gccggttcca
     3961 gatcgttgac gacacgcccg cctatggata tctcgccagc ggtgatttct tccagtcccg
     4021 caaccatacg cagcagggtc gatttaccgc agcccgaggg gcccacgatg acgatgaatt
     4081 cgtggtcggc aatctcggca ttgacgccgt ggatcacctt gaccgcggtt ttgccggcgc
     4141 cgtagtgctt gacgacatct ttgagttgaa tcgaagccat gttctgtttc cgtcttattt
     4201 ttctgtatcg accaggccct tgacgaacca gcgttgcatc agcatgacca ccagtgcggg
     4261 cggaatcatg gccagcacgg cggttgccat gacgatgttc cagtcggtgg cggcatcgcc
     4321 accgctgatc atgcgcttga tgccgatcac cacggggtac atggtttcgt tggtggtgac
     4381 aagcagaggc cacaggtact ggttccaccc gtagatgaac tggatcacga agagcgcagc
     4441 aatgctggtg gttgacagcg gcaccagcac atccttgaaa aagcgtaatg gcccggcacc
     4501 gtccatgcgt gccgcttcca ccagttcgtc gggcacggtc agaaagaact gtctgaacag
     4561 gaaggtggct gtcgccgagg caatcaacgg gatggtcaga ccggcgtagg tgttgagcat
     4621 gcccaggtcg gacaccacct ggtaggtcgg accgatgcgt acctccaccg gcagcatcag
     4681 cgtgacaaaa atggcccaga aaaagaacat gcgaaacgga aaccggaagt acacgatggc
     4741 aaacgccgac agcagcgaga tgacgatttt gccgatcgcg atgaccagtg ccgtgatcag
     4801 gctgaccatc atcatgcggc ccaccggcgc attcgaggcc gcgcccatgc cggtgccttt
     4861 gagcgcagcg gtgtagtttt cgaccatgtg cgagccgggc agcatggaca tcggtgcctg
     4921 caaaatctcg tcggccgtct gggtggaggc gacgaaggtc acatacagcg gaaacgccac
     4981 gacaaacacg ccgatgatca gtacgaggtg cgcgaggatg cgcaggaaag ggctgcgttc
     5041 gaccatcagt actgcacctt cttttcgacg aaccggaact ggataacggt cagaatgatg
     5101 acgatgaaca tcagcaccac cgactgcgcg gccgagccac ccaggtcgag cgccttgaag
     5161 ccatcgtaat agaccttgta gacgaggatg gcggtatccc ggccggggcc gccttcggtt
     5221 gccgcgtcca cgatggcgaa ggtgtcaaag aaggcgtaga ccatgttgat gacgaacaga
     5281 aagaacgtgg tgggcgacag cagcgggaac tggatcgacc agaagcggcg ccatggaccc
     5341 gcgccgtcaa tggcagcggc ctcgatcagc gatttgggaa tactctgcaa tccggccagg
     5401 aaaaacagga agttgtagga gatctgcttc cacaccgccg ccaccacgat cagcgtcatg
     5461 gcgtggttgc tgttgagtag gctgttccag tcgatgccca gcttgcgcag cgcgaacgac
     5521 acaatgccaa tggatggcgc aaacatgaac agccacaaca cccccgccac agcgggtgcg
     5581 acggcgtagg gccagatcag cagggtgcgg tagatccccg ttcccttgac caccctgtcg
     5641 gcaaacacag ccagtaccag cgacaaccca atccccaggc ccgccaccag caccgagaag
     5701 atggcagtga tcttgaacga ggcgaggtag gttgggtcgt gcagcaggtt ggtgaagttc
     5761 ttgagcccca cccattcggt ggacaggccg aaagcgtcag attgctggaa cgattgcaac
     5821 agggcctggg cggccggcca gaaaaagaag atggcgatga ccgcgacttg gggcgtcaac
     5881 aacacccagg gcagccatgc cgaacggaaa acaacgcgtt tttcagaagc cacagtattt
     5941 aaatctggaa acgccgcgcc ccgggctgga gcgcggcaat cggagcagcg agtttagtgc
     6001 ctgagcacgc ccgactccgt acgcgttact tgttggcctt ctgaaaacgc tcgagttgct
     6061 cgttgccgcg gctcacgatg gcgtccagtg cttccttggc tgtcttcttg ccagcccaga
     6121 cctgctcgag ttcttcgtcc tcgatggcgc ggatctggat gaagttgccc aggcgaatgc
     6181 cgcgcgactt gtccgtggtt ttgcggatca tctggttgac agccacgtcg gtgccgggat
     6241 tttgcttgta gaaacctgac ttttcggtca tgtcaaatgc agccagggtg atcggcaagt
     6301 agccggtgcg ctggtggctc ttcgactgga tctcggcact ggtcaggtag tggaagaact
     6361 cggccacgcc cttgtactca gccggttttt tgccgcccat gacccacagc gaggcgccac
     6421 cgatcacggt gttctgcggc gcgcctgcca catcggggta gtagggtagt ggcgcgatac
     6481 cgaagtcgaa tttggcgttt ttcttgatgc tggcgtaggt tgatgccgag gccgtggcca
     6541 tggcgcattc gcccgaatag aacggtgcgt cggccttgtt gccgcggccg cggtagacaa
     6601 acaggccctg tttggccatg ttggacaggt tctcgatgtg acgcacgtgc agcgggctgt
     6661 tggtgaccag tcgggcgctg gtgccgccga agccgttgcc tttggtcgca aattcgacgt
     6721 tgtgccaggt ggagaagctc tccagctggg tccagccctg ccagctggtg gtgaacggac
     6781 agttcacgcc agctgccttc agcttggccg ccgccagcac gacttcaggc caggtggc
//
LOCUS       DAIDFG010000170         6790 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_170_length_6790_cov_17.293838, whole genome shotgun sequence.
ACCESSION   DAIDFG010000170 DAIDFG010000000
VERSION     DAIDFG010000170.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6790)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170_length_6790_cov_17.293838"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1043 a         2183 c         2317 g         1247 t
ORIGIN      
        1 caatacccac gtcggccgcc ttgagtgcgg gcgcgtcgtt gacgccgtcg ccggtcatgg
       61 cccatcatcg cgctggcgct ggtgcccacg ctgctgcact ggccggtgct gctgctgccg
      121 gtgcacatcg tgttgctgga actgttgatt gatccggcct gctccatcgt gttcgaggcc
      181 gagccggagg gcagcgacct gatgacgcga ccgccgcgcc ccgcctcgga ctcaccgttt
      241 gccgccgcac cgctgatgca gggcgtgttg cagggcctgg gtgtggcagg ggtgctgctg
      301 gccgggcaga cctggctggc agggcagggc tggagcgccg cccagggccg tgccgtggtg
      361 ttcagcgcgc tggtgctggc ggtgatgttg ttgatcctgg ccaaccgcga tccgtcgcgg
      421 cccgtactgc tcggtgcggc tgcgtccaac ccctggctgt ggcgcatggc cgccggtgtg
      481 gctgtgctgc tggtggcggt gctcggcctg ccgtggctgc gggacctgat gggtctggcc
      541 ctgcctggag ggcgggaact gacactcgcc gccgtgctgg tggcactgtg tctggtctgg
      601 ctggaactgg tgcgggtgct ggggcagcgc ctgttccgta gccacagaat ctccgactga
      661 acccagcgcg cgttgcgcat tcgggccatg gccgctgcct gagaagcggg cagtgccggg
      721 aaggggctgc cgcgtcaggt gttctgcggt ttccggccct gttgcagcac ttcccgcagc
      781 ccggcgaaac gcttgcggta ctgtgcaggc gtcaggttga cttcacgctt gaacagccgc
      841 gcgaagtagc ccgcgtcttc gtaacccacc tgctcggcga tggcctcgat cgagtcgtcc
      901 gaggcttcca gcaactgctt ggcttcttcg agccgcacgg tgtgcacgta ctgcagcggc
      961 gacatgcccg tggcctgctg gaagcgccgc acgaaagagc gttctgccag acccgacagc
     1021 tgcgtcatcg ccgccaccgg actggacgcc cggtagtgct ctgcggccca gagctggcaa
     1081 cgcgtgatga cggcgtcgtc cgattggcgg ctggctgaca ggcgggcaaa gggctgctgg
     1141 ccgctttggt gccagtcgat gaggtacagg cgcgccatct gcatggccgc ctcaaggccg
     1201 atcaggcgcg cgatcaggta gagggccagg tcctgccagg acgtgccgcc gcccgccatc
     1261 accagccgtt gaccttcacc cgcagtgacc agtgcgcgct ggcggtgcag ccgaatgccc
     1321 ggatggcgtt gtgccagcac gtcgcaatag gcccagtgtg tggtggcgtc ttcgccctcc
     1381 agcaggccgg cttcggccag caacagcgca ccggagcagg ccgccgccac gatggcgcca
     1441 cgggcatacg tctgtctcag ccaggcagcc tcggccttga agcggccgcg caagtcatcg
     1501 gtgggaacca caaacacctc gggcacacac accacctcgg cgggcagtgc atcggccagc
     1561 gacgcatcgg gcaccacccg cacgccgttg gcaatggtca ggggctggcc gtcgctcgag
     1621 acgatttgca cccgaagctg cgatgtgccg gggcggcctt cgaccacctg cggccagtcg
     1681 cgtccgacac cctgaaacag gtcgttcagg ccaaacagca ccgaggcggt gacctcgggc
     1741 agcaccagga tggaaacggt ggcgatggcg cgggtaccgg ttggctcggt catggcggat
     1801 ttgtcgtgtg ggtggcggtt ttggctgcag gtcaaaacgg ccttcctgcc tacagttgct
     1861 gaacattcta gagcgctgct tccgccatgc gggccggcac tgctttcagt ctgactcgcg
     1921 ctttagtcaa ccctacaagg aaatcgacat gtctttgccc gacatcctga cgctggctgt
     1981 tttgcccgcg ttcctgcttc tggactattt tgtccctccc gccggcgggt ttcgcagccg
     2041 gtggtggcgc tcccgcgccg cggtggtgat tgccatcaac ttcggaatgg cgctgctggt
     2101 cggccagctt tgggccgggt tcatgggtga tctgcatgtg ttcaacggcg cgtcgctcgg
     2161 caccgcgggc ggcgctgcgg cgggtgtgct ggtgtatgag tttttccatt actggtacca
     2221 ccgcagcgcc caccgcttcg actggctgtg gctggcaggg caccagatgc accacagcac
     2281 cgagtccttc gacgcctggg gcgcctttta cctgcatccg ctggacacgg ttttcttcac
     2341 cacgctgctg agcctggtgc tgttccccct gctgggcctg agccccgagg cgggcgcctg
     2401 ggcggcggcc gtgctgactt ttctgagcgt ttttgagcat gcccgtgtgc acacgccgcg
     2461 ctggctgggt tacctggtcc agcggcccga gagccatgcc gtgcaccacc agcgcggtgt
     2521 gcacgcccac aactatgccg atctgccgct gtgggacctg ctcttcggaa ccttccacaa
     2581 cggagccgta caaggcccat ggcctgaaca aggttttcac gacggcgctt cggcgcgtat
     2641 tcccgaaatg ctggcgtttc gcgacgtgtc gcgcccggca ctgattcaac ccacccactc
     2701 tttggagatt tccccatgaa agacgcttcc ggatacaccc tctcgggctg caatgcccag
     2761 gccctgagcc accttgagca ggccctgcat gaatttcgct gtttcgccgg caacccggtg
     2821 gccacggccg aggctgcgct ggccgaagcg cccggcctgg tgatggcgca tgtgctgcgc
     2881 gcctggctgt acctgctttc cgccgaggca gatggcgttg tgcccgcacg cgaagccctg
     2941 gacgcggcgc gtagcctgcc ctgcaacgaa cgcgaagcct ggcacctggg tgccatccag
     3001 cagctgtgcc atggccactg gcgcgccgca gccatcacgc tggaagacct caatgcccac
     3061 tacccgctgg acacgctggc cctgcaggcg ggccagcaga tcgacttctt cgcgggcgac
     3121 tcgcgcatgc tgcgcgaccg catcgcgcgt gtgttgccgg catggtcccg ccatgtgccc
     3181 ggctggcacg cggtgctggg catgcaggcc ttcgggctgg aggaaaccgg cgactatgcc
     3241 cgcgcggagc gcctgggtcg cgaagccgtg gagcttgagc cgcgagacgc ctgggcccag
     3301 cacgcggtgg cgcatgtgct ggagatgcag gggcgccgtg acgaaggcat tgaatggatg
     3361 cttggcaacg aaggctggca gtcggacagt tttctggccg tgcacaactg gtggcacctg
     3421 gccctgcacc acctcgcgca gggcgacgtg cccgaggtgc tggcgcttta cgacggcccc
     3481 atccacggcc accgcccgca gattcatgtg gagctggtgg acgccagcgc cttgctctgg
     3541 cggctcaagc tccagggggc cgacgtcggc cagcgctggc agcaactggc cgagcgctgg
     3601 gcgccatttg ccgccgatgg ccactacgct ttcagtgact ggcatgcggc gatggccttt
     3661 gttgctgccg gacgtgacga cctgttgcag gcgctgcaac acgcccagca agaagccatg
     3721 acgcgccagg gcgacaacgc cgtattcacc cgtgaagtcg gtgcggccgc cacgcaggcc
     3781 ttcatcgcgt acgggcaaca ggactggggc cgtgcggtcg aactgctgcg gcccatccgc
     3841 agccagtcgc atcgtttcgg cggcagccac gcgcaacggg acttgctgga gcagacgctg
     3901 attgcagcag cgcagcgcag cgggcagcgg gtgttggccc aggcgctgga gcaggagcgc
     3961 cagctgctgg cgcggcaacg gcaccctgtc actcatgcgg gtcttgttgt cacatcatga
     4021 gcacggcatt cactttttcg cgcttgtttc cataccgtcc cgcctggccc tggcaacggg
     4081 agggggtggc ctggcccggt tcgacggaca ccctggactt gtcgaagtca gccatccacc
     4141 gcattgcgcg gcctgcgggt tatcgcattg aatgcatcgc cggttcggcg tgggtcacgc
     4201 aggacgggca tgcgcgggac atcatgctgg aggccgggga aggctacacc tgcgtgcggc
     4261 ctgagcgcct tctggtgcag gccctggagg cggtggtgct gcgcgtggcg ttagctgccg
     4321 tgtgacccag ccatcagccc cgtgccggta tgcccaggcc tttgacaacc gccggccgcg
     4381 ccacaaacgt ttccagcaca cgcagcacgt tcggaaagtc ggtgatgccg atcagctcgc
     4441 cggcctcgta gaacccgatc aggttgcgca cccagccgaa ggtggcgatg tcggcgatgg
     4501 tgtagtcgtc gcccatgatc caggcgcggc cttcgaggcg cttgtccagc acaccgagca
     4561 gccgttttgc ctccgccaca tagcggtcgc gcgggcgttt gtcgtcgtag tccttgccgg
     4621 cgaacttgtg gaagaaaccg agctggccga acatcgggcc gatgccgccc atctggaaca
     4681 tcagccactg gatggtttca taacggccgg cagggtcggt tggcatgaat ttgccggtct
     4741 tctcggccag gtagatcagg atggcgccgg actcgaacag gcccagcggt tggccgcccg
     4801 ggccgttcgg gtcgaggatg gccgggatct tgttgttggg gctcagcgag atgaattccg
     4861 gcgacatctg gtcgttggtc tcgaagctga ccaggtgcgg ctcgtaaggc aggccggttt
     4921 cctccagcat gatggacacc ttgacgccgt tgggcgtggg cagtgaataa agctggatgc
     4981 gctcggggtg ctgcgcaggc catttacggg tgacggggaa ggcagagaga tcgttcatgg
     5041 cggggtggtg ctgtctgggg gatgttggtc gaagggccac tgtagcccaa gcccgcgaca
     5101 cgcgtattgc gcgcggcggc gaggccgcag gccgaccatc accccgcagt caccgagcgg
     5161 acgggccgca agccgcgcgc cggttagcat gaggaatgcc tgaagtcact ccttcctctt
     5221 catccgccat ccccgatggt tttgttcctg tgcgtgtggg cggccccttt atcgaggtca
     5281 acggcccgtt ctatgcgcgg ctggacggtc agcgcctgct gctgggtttt cgtgtcgagg
     5341 aacggcacag caacccgctg cagatgtgcc acggcggcat gctggcatcg tttgcggaca
     5401 tgctgctgcc ctgcgcggcg atgtaccaga gcgacagcga caggcgcttt ctgccgacca
     5461 tcagcctgca gatcgattac ctggcgccgg ccatgctggg cgcctgggtg cagggccagg
     5521 ccgacatcct gcgcagcacg cgcaacatga tattcataca gggcttggtg acggccgacg
     5581 gtgagccggc cctgcgcatc agcggcattt tcaagcaggg cccgctgatc ggcgacggca
     5641 aggaccgcga cccattttcg ctacgaaatg gatagctgct ggcgcaaaca gtcaaaaggc
     5701 tggagccgtt tttcttcttg aaatgaacca ggcgcccccg gtctgcggcc ggtgacaggc
     5761 ggtgcctcga cgcagatttt tccggtggcc atgcgcaccc acgcgggcgc ttgtggcggt
     5821 ggcccccaaa ggcctgcacc ggcctggtcc ggatgatttg ttctggcacg cgtgacggct
     5881 ttgcaggaca atttgacgca tgaatggcca cccattccca acgcgcaccc ttcaagcgga
     5941 ggtctcccca tgagctggca aatcatcccc ctggaagagg tggcgcccac ggcctggcgc
     6001 aatggcggcg gcatcacgcg cgagctgctg gcctggccga ccgccgggga ctgggactgg
     6061 cgcatctcgg tggccgagat cgaaaaaaac ggaccgtttt cgcgttatga cggtgtgcag
     6121 cgatggttcg cggtactggg cggcgccggc gtgcggctca cgctggacgg gcaaacccgc
     6181 gagttgacgc cgcaaggcga gccgctgtgt tttgacggcg ccctggcctg taactgcgaa
     6241 ctgattgacg gtcccaccga agacttcagc ctgatggtca ggaaggggcg tggcagcgcg
     6301 cgcctggtcc gtgtgctggg ggcgctggac gtggaggtca aacccggcac acgggtcgcc
     6361 gtgtatgcct gggacgagcc ggtgaccgtg caggtcaaca ggaagttcac gacggtgccg
     6421 ccgcacaccc tggcctggcg tgcgctcgcc gatggggctt cggtgcgcat tgaggccatg
     6481 accgcgctct ggatggaaat ggcgcgatga cggtgcagcc cgcgcaaccg gcccatgcct
     6541 tcggcgccaa tccgcgcctt ccaacggttt tcagaggtga gctggcatga gttttaccct
     6601 gcaactcggc aaggcccccc tgctggtcag catgccgcac accggtaccg acattccgcc
     6661 ggagctgcaa ggcgactacg ccgaacgcgc actgggcctg gaggacaccg actggcattt
     6721 gcagcggctg tacgattttc tgccggcgct gggcgccagc gtgatcacgc cgcggtattc
     6781 gcgttatgtg
//
LOCUS       DAIDFG010000171         6680 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_171_length_6680_cov_19.081962, whole genome shotgun sequence.
ACCESSION   DAIDFG010000171 DAIDFG010000000
VERSION     DAIDFG010000171.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6680)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6680
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171_length_6680_cov_19.081962"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1208 a         2134 c         2176 g         1162 t
ORIGIN      
        1 atggattgct gcagggccgc cggcaagcgg ctggcaaagg tgcccgcggc gagatacccg
       61 tcggcgtcga acttcggaaa cgcgcctttt tctttcgcca gcgcgaccga ggcggcgtag
      121 gccgcatcgc gcatggcgat ggcaatacgc gccgccatct cacggccctc gggcgcgtcg
      181 tagcgcaggc acagcatggc cagcgcattg cccagtccgg tgaaaccgac gccgatgcgg
      241 cgcttggcag ccgactcggc ctgctgctgc ggcaacggcc agaaagtcac gtccagcacg
      301 ttgtcgagcg cacgcacctg ggtggtcacc gacttggcaa aagcctcgaa atcaaagtcc
      361 ggcacgccat tgaagccaaa ggggtgacgc acaaaacgcg tgaggatgat ggggccgagg
      421 tcacagcagc cgtagggcgg caagggttgc tcgccgcagg gattggtcgc cgcaatgtct
      481 tcgcagtaac gcaggttgtt gtcctggttg atgtggtcca gaaacaggat gccgggctcg
      541 gcgaagtcgt aggctgaatg catcacggtg tcccacaact cgcgcgccgc taccgtctgg
      601 tagacccaca ggccgtcggc acgctggaac gcaccttgcg cgatcagcgg cgcaccgggt
      661 ttggccttgt gcaccagctc ccagggctgg tcttcggcca gggcgtgcat gaatgcgtcc
      721 ggcatgccga ccgacacatt gaagttgttc cagcgtccgg gggtgcgctt ggcggtgatg
      781 aactcgtgca catccgggtg gtcgatgcgc agcacgccca tctgcgcgcc gcgccgtgaa
      841 ccggcgcttt ccaccgtgga acaggactgg tcgaacacgt tgatgtagct gcacggaccg
      901 gaggccatgg aggctgtcgc cttgacctcc gcgccgcgcg gccggatgcg tgaaaaatcg
      961 tagcccacgc cgccgccgcg ccgcatggtt tcggccgctt cacgcagggc ctcgtaaatg
     1021 ccggggtagc caccgtcatc gacgccctgg atgcagtcgc ccacgggctg cacaaagcag
     1081 ttgatcaggg tcgcctggat ggccgtgccg gccgcgctca tgatgcggcc ggcgcctatc
     1141 gcgccggcgt gcaggttctc gaggaacagg gcctcgtgtt tggcacgttc cgcttccggc
     1201 tcgaccgagg ccagggcccg cgccacgcgc cggtacaggt cttccacacc cgactcgccg
     1261 ggcttgaggt atttttcctt cagcacgtcc tggctgatgg gctgggtggc cgtcatgtcc
     1321 tgaggcctgc cgaactgatc gcgtttcatg cttgtgctcc tgcgatggag actgctggcc
     1381 ggattccgcc gagcagccat tgcatcaatt cttgcaccga acggatctgg tcgccaaacg
     1441 gcaggcgggc agcaccacgg aagaaaaggc ctttacgcgc attgccgtca aacgcatggc
     1501 cgagctgctg gtcgatgcag aactggccca tggtggccac gccatcgcgc aggccgcagt
     1561 gggccaggca gtcaaacgac atgttgcatt tggccttgac atgggccgcc gcctggagtt
     1621 tgggctcgag ccggatgtat ttgtccagcc agggggtgcg caccgcgcgg gccggcaggc
     1681 cggccacact gacgaactcc accatgtctt ccggtttggc ttcggccagc accttcttga
     1741 aggcgggatc ggcatcacac tcctgggtga cggcaaaagc cgttccgagc tgcacggccg
     1801 aggcacccag ggcctgcagg cgcaggatgt cgtcccggca attgatgccg ccggcggcaa
     1861 tcagcggtat ttcacgctcg atgcccgccg tcttgaagaa ttccagcacc tgcgggatga
     1921 ccacctcgaa gtcaaaacgc gggtcatgca ggtccgcaac cttcgctgca cccagatgac
     1981 cgcccgcgag gccggggtgc tcgatcacga tggcatcggg caggcggccc tttttctgcc
     2041 acttgcgcac cagcaattgc acgcctcgcg cgtcggacag gatgggaatc agcgcgacct
     2101 ggggatagtc acgcgccagc tccggcaaat ccagcggcag gccggcgccg accaccaagg
     2161 catcggcacc gctggccagc gcctggtgca cgtaatcctc gtaggcaccg agcgccttca
     2221 tgatgttcac ggcaatcagg ccgcggcctt gtgccagctc acgcgcgcgg cggatttcgc
     2281 ggtccagcgc ctcgagattg gccgcattga tcacggcttt cgcatcgggc tccttgtcca
     2341 ggtggcccgt tttggccatc aggtccggat gcaggcgccg caagtccacg gaagacaccg
     2401 tgcccacccc gcccaggcgg gctaccgcgc cggccaggcc accagccgac acacacacac
     2461 ccatgccgcc ctgcaccacc ggaaccagtt ccttgccgcc aatccgccag ggccgcaggc
     2521 ccgaatcgtg ggcaagtgtc tgcagtatcg cggcggccgc cgccgaatcg acacttgcct
     2581 tgacagccag gtcagtcgct aatgatttgt ccatcgtgtt ccacataagg ttggtacggg
     2641 ccagtgccgc tgttggaggc tatcgcagcg ggcacaaaac ttccttgccg tacatcaaag
     2701 atgtggattt caccgtcctc aatcacgtaa tgccagccat gcagcgtcaa aacgcccttc
     2761 tcgacctcgc gccgcaccat ggggtaggcc atcaggcgct cgagctgcag caccacgatg
     2821 cgctgttcgg tgcggcgcag ggcctcggga ctggcctgga ccggcagcaa ggcttcgctg
     2881 gccagtttga gccaggcctg cagggccaca gcttcttcgg gcacgccttc ataagcggcc
     2941 cggatggcgc cacagtggct gtgaccgcag acgatgatgc gctccacatg caggctgagc
     3001 accgcgaact cgatcgccgc cgtggtgccg tgcaatccgt gcgagccgtc gtacggcgga
     3061 acgaaggcac ccacgttgcg cacgatgaag agctcgcccg ggccggcacc ggtcaggagg
     3121 tagggcacca cccgcgaatc cgagcagccg atgaacagtg tcttgggatg ctgcccctgc
     3181 gccaccaggt cctggaaacg ctgctgatgc agcggaaaat agtcggagtg aaaacgccgc
     3241 aagcgaagca gcaactcgtc cgcgcttgca acgccttcgg tcattggacc agcggcgaat
     3301 cagccccttc cagggcaatg ccttcgacct ccgcctctcc caccagcagg ctcatgtact
     3361 ggtgcagcgc ccgcgcacgg gactgcatgg tcagcagggc cgcaatacgc tcgcgcactt
     3421 cttcataggc gggctgggtg ccgctgcgac gctccagcac gtcgatgatg tgaaaaccga
     3481 aacgcgtgtg aaacaggcgc ggatgcacac cggtgccggt ttgcgcatgc tggaggtgaa
     3541 acagctccgt tgccagctcg ggggcacagt cgtccggcgt gatccagccg aggtcgccgc
     3601 cctgggcgct gctcgggcag ttggacaact cggcggccaa ttgggaaaac cgctccgggg
     3661 acaccccctt gtgcgacagc tccagcaagg cgcgttcggc atgcacggtg agtgcctgga
     3721 cgttgacacc gggcgtgacg gcaaacagga tgtgccgcac atgcagcgcc tggcccacca
     3781 gaaactgcgc cttgtgggct tcgtaataac gccggccttc gtcgtcactc ggctgggggc
     3841 tgaccacctg ccggtccacc atggcttcga tgatttcgcg ttcggcctgg cccagttccg
     3901 ggctggtcaa gccggtgtga cgcggcagca ggccttgcga caccgcctgc tggcgcagca
     3961 gttcggtgta ggccagctcg cgcagcgtgg ccgtgtccgg acgctgaccg gtcgggtgca
     4021 gagcgatgcc gttgacgcag gcggtgggct gaactggctg ggcggctgca gggccgcact
     4081 ggcaggagcc gctgccgcaa ccctgggctg caacgggtga agaaagcgtt tgtgtggtca
     4141 tggagagttc ctggttcacg gtgaggtcaa cgggcacccg gcgatttggc ccgcggaatg
     4201 cccaggcgac ggctgcgcac catctggtag ggacggaaca ggtaggcgac cgctccgaag
     4261 ccgctccaca catgcaccag acggctgaac gggaacagca ggaagatagt catgccgaac
     4321 acgatgtgga aacggtaggg ccagggcagg acggccagcg ctgcagagtc cacctcacgc
     4381 agggtgagaa tgcgctgcgc ccattcggcc aggatcagca tcacgctgcc atcggcgtgt
     4441 ccccacgaat acggcagggt gatcagaccc accaccagct ggacccatag gatcagcagg
     4501 atcgcgagat cggtcctgtg gctggtgtaa cggatgcgcg gatcgaacat gcggcggtaa
     4561 atcagcatcg acaggccgat gaagcagatg acgccggcaa tgccccccgc gtagatggcc
     4621 atgcgctgct tggtcgctgc gtccatgaaa aagccataca ccgcgtgggg tgtcagcagg
     4681 ccgaccagat ggccgaagaa caggaacagg atgcccacgt ggaacaggtt gctgccccag
     4741 cgcagcaggc cggtgcgcag caactgcgaa gagtcgcttt tccaggtgta ctggtcgcgg
     4801 tcgaagcgga tcaggctgcc gagcagaaac acggtcaggc agatgtaggg atagacacca
     4861 aaaagaaatc cgtgaagtcc ggggctcatg cttgtactcc ttgggtgggt cgtgccttgc
     4921 gtacgtgcgg cacgatgtga atcggttgcg gctggtccgg cctggcctgg cctttggacg
     4981 agcagccgtc aaaaaccacc ggctcttccc agaccgcgtc gacaggttct tccgccgcga
     5041 tctgcaccgg ctgggccttt tcccccgcca gctccagcaa ggcgcccagc acggaggcat
     5101 aggcactttc gcgctgctgc agggccgcaa aaattgcatt gaagatgtgc gccatctcgg
     5161 ccagaaactc gcgcgcctcg cgcggtggct gggtcgaggt gaattcaagc accaccggca
     5221 ggaagtccgg catctcgcct tctgccaggt acatgccggc cttctcgtag gtctgcgcga
     5281 ggtcgatcat ggccggcccg cgatcgcgcg agtcgccgtg cacgtgttcg aacaggtgca
     5341 gcgatgtggc gcggccccgg tcgaacaact cgacatacgc cgcctcggtc acaagcgccg
     5401 gcgcaagcgc gagcgcttcg atcaggccat ccagctcggc cagccgcagc ggctccaggg
     5461 cacgttcttc gtgcagtgcc tcgcgcagcg cgcccagatg gctgcgcagc tcggcgtcgg
     5521 ggtaagcgag cagccgggcc agcacacgca ggctcttcga agccacagcg ggggtgttgt
     5581 tgaaaagtgc catggttcag acctccacct tgatgggaat agtgcgtttc ttgttgccgc
     5641 cgaacaggct gacgtcgctg gcgccgtcgg agcagccgtt gccgaaggtg aagccgcagc
     5701 cgccgcgcat gtcgaaggcg ttctcggcgt attcgcggtg cgtggtcgga atcacgaagc
     5761 ggtcttcgta gttggcaatc gccatgatgt gatacatctc ctcgacctcg gccacggtca
     5821 atccggcctg ctccagcacc gccaggttct gcacctggtc cacatgtttg ctgcgctggt
     5881 aggaacgcat cgccagcatg cgctgcagtg agcggatcac cggtgctgtg tcgcccgcgg
     5941 tcagcaggtt ggccaggtac tgcacgggaa tacgcatctg cgcgatgtcg ggcaactcgc
     6001 cgttgatgcc gatctggccg gcgttggccg ccgaggtgat gggcgacagc ggcggcacgt
     6061 accagaccat gggcagcgtg cggtactcgg ggtgcagcgg cagcgccacc ttccactcca
     6121 cagccatctt gtagaccggg ctgcggcggg cgccttccag ccaggcttcg ggaatgccgt
     6181 ccttgcgcgc ctgttcaatc actttcggat cgttcgggtc caggaagatg tcgagctggg
     6241 cctggtacag gtccttgtcg tcttccacgc tggccgcttc ctggatgcgg tcggcgtcat
     6301 acagcaccac gccaaggtaa cggatgcggc ccacgcaggt ttcgctgcag acggtcggct
     6361 ggccggcttc aatacgcgga tagcagaagg tgcatttttc ggccttgccg ctcttccagt
     6421 tgtaatagat ttttttgtac gggcagcccg agacacacat gcgccagccg cggcacttgt
     6481 cctggtcgat tagcacgatg ccgtcttcct cgcgcttgta gatcgagccc gaaggacagg
     6541 acgccacaca ggccgggttc aggcagtgct cgcacaggcg cggcaaatac atcatgaagg
     6601 tgttttcgaa ctggcccacc atgtctttct ggacctggtc gaagttcgcc aggttggcgt
     6661 ccttgctgcg cttggaaaac
//
LOCUS       DAIDFG010000172         6669 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_172_length_6669_cov_19.135773, whole genome shotgun sequence.
ACCESSION   DAIDFG010000172 DAIDFG010000000
VERSION     DAIDFG010000172.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172_length_6669_cov_19.135773"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1194 a         2171 c         2017 g         1287 t
ORIGIN      
        1 aacccccaag cccgtcaggg tatgggggct gccaggacag gcttctgcgc agtcggcggg
       61 catggttgcc cacccctcgc agcccttaac gcaagggacg gatcagcgcg aactgcgcca
      121 cgtcgccgcg ccggaacagc acgttgatca ccttgctctt gtcggttttg gccagcaccg
      181 cttcaaagtc cttgacgctg gtgacctccg tgtttgcaat cgccagaatc acatcgccct
      241 cacgcaagcc ggcgcgcgcg gcggcgccct cactcgcatc caccttgacg ccgcccttga
      301 gcttgagctc cttcttcagg gcctcgggca actcgctgac caccaacccg atcacctgcg
      361 ccggtccggc caccggtgct ttgctctcgg gtgcctgggc cttgcgcacc ggcttttctg
      421 cctccacctc ggcaatcgtc acgttgaagt cccgggtact gccgcgcctg aagacggtga
      481 tcgtggtttt ggtaccgggc ttcacattgc ccaccatacg cggcaggtcg gttgcccggt
      541 caatcacctt gccctcaaat ctggtgatga tgtcgcccgc ttcgatcccg gctttttccg
      601 caggcgatcc ggcttcgacg ccgcgcacca gcgcaccttg cggtttgccc agtccgatgg
      661 actccgccac gtccttgctg acctggtcga tctgcacgcc gatgcgaccg cgcgtcaccc
      721 gtccattggt gcgcaactgg tcggccacgc gggtggcctc gtcaattgga atggcaaacg
      781 agatgccctg gaagccaccg gagcgcgagt agatctggct gttgataccc accacctcgc
      841 cacgcatgtt gatcagcggg ccgcccgagt tgcccggatt gatcgccaca tcggtctgga
      901 tgaaaggcag gtagtcgccg gtgtcgcgct gcttggcgct cacaatgccg gcggtcaccg
      961 tgttctcaag gccgaaaggc gaaccgatcg ccatgaccca ctcccctact ttcaggcggc
     1021 tgatatcgcc gattttcacg gccgacaagc ccgcggcctc gattttgacc aaagccacgt
     1081 cggtgcgctt gtcggtaccg atgatcttcg ccttgaactc gcgcttgtcc gtcagcgtca
     1141 ccagcacctc atcggcgcct tcgacgacat gggcattggt catgaccagc ccgtcggtgc
     1201 tgaggatgaa tcccgaaccc acgccgcgcg gctgctcttc gtcaggctgg ccacggtccg
     1261 gcttgggtgt gcgcggcatg ttgggcggca ccggaatgcc gaagcgccgg aaaaattcca
     1321 gcatctgctc gtcggcgctg ccggctgctc cggccttgac tttttcaacg gtccggatgt
     1381 tgacgaccga aggaccgacc gcttcgacca ggtccgtgaa gtccggcagg gtcctgactt
     1441 gcgccatcac cggctgcaca ggcatcacgg ccaggacggt gcccaacgcc agcgtaccgg
     1501 cgacgaggca ggagcgacct tgtttccagt tcaatataaa catcatcagc ctttgaaaaa
     1561 attgtcggga aagaagagaa acccgcgtgt tgaatcaggt gccggccggc ccgcgacaac
     1621 aggcaagggc caggacgaca tgggtgcgct atttcctgcg ttcaagacca ttggcaaaca
     1681 ggctcagggt ggccatgggc acctcgccca tcactgtcag ccagtaagcg tcgacacgcc
     1741 gggtcagcga ctgcgtggcc cccagggaca ccctggattc ccggagaggg cgctgggcat
     1801 caaacgactc gacaaaaatc gataccgacg ccaggccgtc cgaaaaaacc cactgcatgg
     1861 gctcgcttcc cgactgcccc gcgagcgggc gcttgtagca actcatggac tgaaagcccg
     1921 gcaccggcgt tttcatcacc cagccttcgg cgctggccgt ggtcttgacc agtcccggtt
     1981 tttcgacccg gtagccatcg accttggcca tcatttgcgc cagcttgtcg atcctgaccg
     2041 gcgcatcgag ctgcaattcc gaaaaagccg cctgctccag caccttgcca tcggcatcga
     2101 gcgtctgcag cttgacgacc atgcccttct tctgttcggc ccagacgcgg tagccgaagc
     2161 gcatcgtgtc gcggggaatc agggcgacga tgtcggcctc cacccctgcc acccgctcgc
     2221 tgccctcgtg tcgggccttg tagaaatcgg cgatgtggct gtcggtggac ttgagcagtt
     2281 ggggaaaaag gcccagcgac tcctgctttt cggcacgcac caccttctgg tcgggcatga
     2341 aggtcacgac ctggtcgttg tgccggaaaa cagtacgcgg ggccccggtc agggtctcga
     2401 ccttttcgat ctgctgggtt ccgtcgcaga catgccagat cttcgcgctg gacatgacgc
     2461 cgccggagga caccacaaaa gtgcccacgt aagaccggtt tctggaggct tcatgcatgc
     2521 gcatcaacca atcgttgatg ctcctgggct ccgcagcctg gcccggtgct gctgctgttg
     2581 ctgcgggtgc cggggcctgg gccgctacat aatttgcagc aaatagcgca cacgcagcaa
     2641 gggccagcgg gcgaaaatag ctgaaattca tgggatgaac cggcctggtc ttgcctgtgc
     2701 tagccacgtc agcgacgctc gccctggggc gactcgaagg tggcgtttcg caggaagccc
     2761 gagggcatct gcagggccga cgcgccaccc agttgccggt gggccgccag cagttcctgc
     2821 gtcctcgcat cgcgcaccag ggtcccccgc tccgacacca ccagcacctg ctgcgcagca
     2881 ggctccgagc gggccagttg cggtgcagcc gaaggcgaca gcaggccggc gccggcattc
     2941 caggcaatcg ccgccaccgc agcgacggac gcaaaccccg ccaccagctt ccaggggaag
     3001 ctggcgtcgt tggcggcttc gcctacggtc catccggctc gcccgcccgg cgcgtgacgt
     3061 acttccggca gagccggcgc agcaacgatg accggctcct ggctcaggcg cacccgcagg
     3121 cgcgccataa aggccgtatc cgctgcgtgc gggctcagtt ccggcgaacg caacacatcg
     3181 ccaatcaggt gataggtgtt ccagctgtcc agcgcctggg catcggcctc gcaggccgcc
     3241 agcgcacggg caaagtcctc gcttgccagc tggccgtcag ccagggcgga gatcaactca
     3301 ctgtgttgcg tcatcggttt catggtgtcc atgggcagct tgggtctgcc ggtttcaatc
     3361 gtttactctg tcgtttactc tggcttgcat cggctcgtcg ctacttctcg acaaactacc
     3421 agcgcttgcc ggactggttt tccagcaagg gcttgatcct ggaagagatc gcttcccggg
     3481 ccctgaaaat ccgcgaccgc acggtgccga tcgggcagtt catgacttcc gcaatctctt
     3541 cataactcag cccttcaatt tcccgcagcg tgatggcctg gcgcaactcc tcgggcaaag
     3601 cctccatggc ggcgttgacc atttgcgcga tctccttgcc ggccagcacg gcttcgggcg
     3661 tttccgaact gattagttcg ttttccagcg gggaagtttc atcgctttca tcgctttggc
     3721 cggatttgaa ggaattctct gacaccgtgg ggtcgcgctt gagctccatc aaggcctttt
     3781 tggcggtatt gacagcgatg cggtacagcc aggtgtagaa ctgggcttcg cccctgaact
     3841 gggcaagcgc gcggtaggca cgaatgaagg tttcctgggc aatgtcctcg accaggtcca
     3901 catcacgcac catgcgcccg atcaggcgct ggatgcggcg ctggtacttg atgaccagca
     3961 gctcaaaggc cttctggtcc cccgcgacag tgcgctcgac cagcatggca tcgctgtcgc
     4021 ttggcgcggc cggctcctgc ggggactcgt cggtactcat gaaccggagc gcagcgaagg
     4081 agactgcgcc ggaaagtcag agatggtcac gggagagctg gcgtcccacg gcaccaggtc
     4141 cgcagacggc ctgtgctgcg cgtgtacggc gcggcgcaaa tccagccagc gctcgggctg
     4201 ggccgaacgg cccgcccaga gccacagggt ggcgtggtcc tggttttcca gccgcagcag
     4261 catggtgcgc tgcagatcga gtgcgaccac cagatcacgc accggtgtgc ctgactggta
     4321 accctggctt tcccagcgcc agtcctgccc gtcccagtgc agttggccaa tcggcgaatg
     4381 aagccatgcc cagccagcag ccacaccggc agccagcacg gcggccagag ccgtccacaa
     4441 gcgccagtcc gctgcgggtg cagcacgcca ccacagggcc agcacgccgg ccccggcgag
     4501 ccagaggccc agcacggcaa tgccctgaaa aagcgaacgc cccagcgggt aagtaaccgg
     4561 cggggcgtta tgcatggtca gggtgcgaag tgatgggaat caggtgcggc cgggcgtgtg
     4621 gaatggggaa gggcctcaga cgcgcttgaa caccagcgtc ccgttggtgc cgccaaaacc
     4681 gaagttgttt ttgacagcca cctcgatgtt ggcatcacgg gccgtgttcg cgcagtagtc
     4741 cagatcgcac tcgggatcct ggttgaagat attgatggtc ggcggaattt tctggtgatg
     4801 cagtgccagc acggtaaaca ccgactcgat gccgccggcg ccaccgagca ggtgcccggt
     4861 catggacttg gtcgaactca ccaccaggtt tttggcatgg tcgccaaacg cggccttgat
     4921 ggcattcgtc tcgttcaggt cacccagcgg tgtcgaggtg ccgtgcgcat tgaggtactg
     4981 cacgtcgctg gcattgacgc cagcgtcctt gagggccatg tccatggaac ggcgcgggcc
     5041 gttcacgtcc ggcgtggtca tgtgatacgc gtcaccgctc atgccgaaac cgatcacctc
     5101 ggcgtaaatc ttcgcgccgc gcgccttggc gtgttcgtac tcttcgagca ccagcacacc
     5161 gccgccctcg ccgagcacaa agccgtcccg gtccttgtcc cagggacgcg atgccgtctt
     5221 cgggtcttcg ttgcgggtcg acagggcacg ggctgcggca aaaccgccca cccccagcgg
     5281 cgacaccgtc gcctccgcac cgccggccac catgacgtcg caatcaccat gctcgatcat
     5341 gcgtgccgac aggccgatcg catgcaggcc ggtggtacag gccgtcacaa cggcaatgtt
     5401 ggggcccttg aagccaaact tgatcgacac atgcccggag atcatgttga tgatcgaggc
     5461 cggcacaaaa aatggggaaa tccggcgcgg accccggttg atcagctcgg tgtgcgtctg
     5521 ctcgatcatc ggcaaaccgc caatgcccga gccgatgttg cagccgatgc gcgtcgccag
     5581 ctcttcgtcc agcgcatcgc ccgtgggcaa gccgctgtcg gccaccgcct ggcaggccgc
     5641 ggccagaccc aggtgaatga agcggtccat gtgccgggct tctttttcag ggatgtaatc
     5701 cgtgatgtcg aagcccttga cctcacctgc aaatttgcag gcgaagctgc ttgcatcaaa
     5761 ttgcgtgatc aggtcaatgc cgggcgttcc ggcaagtaca ttggcccagg agtcggccac
     5821 ggtgttaccc accgggctga cacaacccag acctgtcaca acgacgcgac gacggctcat
     5881 gcgcaaatcc tgaagaaagt cccgaaagac caaaggcctt tcgagcgagc tgaaaaaaga
     5941 ccggagtctt taggcttttt gatgggtgtt cgcgtagtca atcgcgttct gaacggtggt
     6001 gatcttctcg gcgtcttcgt cggggatctc gatgccaaac tcgtcttcca gagccatgac
     6061 cagttctacg gtgtcaagcg agtcagcgcc cagatcggcc acaaaggctt tttcgctggt
     6121 gacctggcct tcttccacgc caagttgctc ggcaatgatt ttcttaacac gtgcttcgat
     6181 atcgctcata gttccctctg agggttgtga attaatccgt gattttagcg ttgttggggt
     6241 tgatttccca acccgccagc cggacggata aaacccggct tgtggtccgg tgcctgcagg
     6301 ctgcatgcgt caccattatt catgatcctg gtgacgcagg gcagatcggc agccgccgga
     6361 tggtttacat gtacatcccg ccgttgacgt gaagctcctg cccggtgatg tagccggcct
     6421 gcgggctcgc tacaaaggcc accgcatgcg cgatgtcctg cggcttgccc aggtgcccca
     6481 gcggaatctg tcccagcagc gccttctgtt gttcctcggg caaacctgcc gtcatgtcgg
     6541 tttcaataaa gcctggtgcg acgcagttga cggtgatgtt gcggctgccg agctcgcggg
     6601 ccagcgcacg tgtcatgcct gccacgccag ccttggccgc tgcgtaattg gcctggccgg
     6661 cgttgccgg
//
LOCUS       DAIDFG010000173         6586 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_173_length_6586_cov_23.776757, whole genome shotgun sequence.
ACCESSION   DAIDFG010000173 DAIDFG010000000
VERSION     DAIDFG010000173.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 6586)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6586)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6586
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173_length_6586_cov_23.776757"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1198 a         2053 c         2050 g         1285 t
ORIGIN      
        1 taagttcacg ccgctgacca cttaagttca cgccgctgac cacttaagtt cacgcagcaa
       61 cgatggccaa tagtcggcga aacggacggg ggcagatccc gatcagacat ctcgataagt
      121 gacaacctaa tttctcttgt aaatcaaagg cttaggtgca tttctgcgaa attttaagga
      181 atcgcgaccg accctctgaa actggaatca accccgaatt ccaggctcaa aagttcaagc
      241 catcagttct tggacaattt tcactgccgc cgcgtcaagc agctcaaact gcaagccagc
      301 gctgaagcct ttgtcgcgca ccagaatgct gtaaacaacg acggcacgca tgctcaggga
      361 accgcgtctg ctactttgca gcagccgaca ggtttgctgt acctgcttcc tgtgcggcgg
      421 cttggtcgcg tcgcaagtgc ccgatcagcg attgctggtc atcgcgatgg ccccattggt
      481 tgaaatacac caggtcttcc atcggcagcc ggggcagcca ccccgcactt tccagttccg
      541 gtttggcatg gaaatggctg acgtagccga cgcacaggta ggcgatgggc gtgatgcgct
      601 tcggaattcc cagtgcttcc tgcagttcgg cttggttgaa aatgctgacc cagccgacac
      661 ccagcccctc ggctcgcgct gccagccaca ggttttgtac ggcacacacg ctgctgtaca
      721 gatccatggt cttcatgtgg gtacgcccga ctaccacagg accgctgcgc tcgcggtcgc
      781 aggtgatgca gatgccgagg ggtgactcca gaatgccttc cagtttaagg gtccgataga
      841 cagcgcgttt gtccccttcg aacatgtctc ctgcttcggc gtgggctacg gcaaaggcat
      901 cgtgcacccg gcgcttgacc tggtccgact ggactacaag aaaattccag ggctgcatga
      961 aaccgaccga tggcgcgtag tgggcagccg tcaggacacg gctcagtacc tcatcgggaa
     1021 ccggtgtcgg caaaaactgg ccacgcacat cgcggcggga aaaaatggcg cgatagacag
     1081 catcgcgtgc ttcgtctgca aaggcgtgtg tttcctggat ggcttgcgcc ggcattgttt
     1141 tcatgacatt cccctggtgt gaaaataaat gcgctgcctg gccgtgcaac ttgatggttt
     1201 tccaggccgg tcttctgact cggattcatc tgaaccaggc accttcccgg catacgccag
     1261 tggcatcaag cctgggtcat cctccttaca gcgttgggca cgtggcggaa tcacaccgcc
     1321 ttcccgattc tcctgtcgcc ttgcagcgac gggcacctga aagtcgccat tatctatggt
     1381 gaagctgtaa actcagcgtg ttggtgctcg cggcgtggtt cacatgccgc agttcaacgg
     1441 gaagcaggag ggtttgccgt caaccgcaaa cctaacctgc gctgcccccg caacggtaag
     1501 tggacgaatc ctcccgcgat tccgcttcca tcacggccac tgagcgtttg aacatgcgct
     1561 tcggaaggcg atggaggttg ttccaccagc ccggataccg gccaacaagg tggttgcacg
     1621 ctctggcggg caaccgtgac gctcatgcac tgtcggggac gacggtgagg gcttttgctg
     1681 gaggtttcca ctcatgaaaa cttgtatttc cagtgcgcgc cggttggcat tgccattggc
     1741 ctttacgcca actttccttc ttttttccaa atccaaatcc aaattcaaac cgtcgaggcg
     1801 actctccttg agggcacggt gacggcggcg gctgctggcc ctggcattcg ccggacaggc
     1861 gcaggtgttg ctgatggacg aaccgatggc tgatctggat ccgccgcacc ggcatcatgg
     1921 cccaaggtcg cgtcacccat caaggccgtt gccgcgcatt cgcaaggggg cggacaccct
     1981 taacaccctg atctttcact ttttcgtttg acctattcct attaacacca aggagctttt
     2041 atgcacatcg aacctggcct gttagctcaa tccaaattac tttttgcggc gggtgccgcg
     2101 gctgtcgtgt ttgtgcctta cgtgccaaca ctgcttaaaa atccaaccct gtggctgcgc
     2161 accgcgctcg catcggtttt cttctcgctg ttcatgcagg gttttcatat gcaggcgggg
     2221 ccctccgagc tgcattttgt gggtgcgatg cctatttacc tggcgtttgg attcatcccc
     2281 accttgtttg cttttggttt tgggctgctg ctgcaaggca cgctgtttga gccgcaagat
     2341 ctggtccatc tggcggtgaa caccttgtcg ttgacagtgc ccctgatcgc gctgcattac
     2401 accctggggc gcaagctcaa agaggtgacg atcactacgc ttctcaagct cgacggtgcc
     2461 tattacgcgg gtgtcgtggt catggtcggc ttttggctca cgcagacgga agtggctacg
     2521 ccatggtccg gctgggctgt gtttgcctcg tcctacattc cgttcgtggt ggcagagccc
     2581 ttgttcacct ttgccgttct gtggatgctg caacgctttg tgcaaacccg cgtgggcgct
     2641 gtctgcttcg ccgtgcagcc caagctcaag accggtattg cctgatgagt gcgggcaccg
     2701 tctggtttgt gggtgccggt cccggcgacc cggagttgat caccgtcaag ggccgtgcgc
     2761 tgatcgagcg tgccggcgcc atcttgtttg ccggctccct ggtcaacgca gcggccaccc
     2821 gttgggcacc cgggaactgt gtcatcgcgg acagcaagga catgacgctg gagcagatgt
     2881 cggcgtggct gatcgcgcag gccggaaaac atgaaaccgt ggtgcgcctg caaaccggcg
     2941 accccggcct gtatggggcg ctgatcgaac tggtgcagcc gctggacgcc gcaggcatcg
     3001 cgattcaggt ggttcccggc gtgtcttcgg ccatggcctc ggcggcggcg gcggtggaga
     3061 gcctgaccct gcccgaggtc acgcaaacgg tgattttcac ccgggtggag gggcgcacgc
     3121 ccatgcccga aggcgagtcg ctggaagcgc tggctgcgca ccacagcacg ctgtgcatct
     3181 tcctgagcat taccctgatg ggccgcgtca cggcggcctt gacggcggcc ggctggtcgc
     3241 ccgatgcgcc catcgtggtg gtgcacaagg ccagttggcc tggcgaagaa aaaattgtcc
     3301 gcggcacggt cagcaccatc caggccctgt gccgcgaggc caaaattgtc agccaggcca
     3361 tgatcatcgc cagcccgacg ctgggtgcgc ggcaatggac caccctggcc aaatccaagc
     3421 tttacgacgc cagtttcacg caccgtttcc ggcgtgccag cgtccccgtt tcaaacctta
     3481 ccctgggaac cccatgacca cagaaaccat tttgctggtg ggccacggct cacgcgaagc
     3541 ctccggcaac gaggaaatcg agcgctttgc cgcccagtgg cgccagcgcc agccaggctg
     3601 gcgcattgaa gtctgcttca tcgagttttc cgagatcacc ctgagcgagg gcctgcgcag
     3661 ggcggccact gatgcacgcc gcgtgatggt ggtgccgctg attctgaatg cggcaggcca
     3721 cgtgaagatg gacattccgc aggccatcga cgcggcgcgc ctgcgttttc cgcaagtgca
     3781 gttcctgtat gccccgcacc tgagcgcctg cgatcacatt ctcggcgtgg tcaaacgccg
     3841 cctcaagttc gccatgcagg ggctcgatat gccggacccg acgaccaccg gcgtggtgat
     3901 cctggggcgc ggctcatcgg accgccaggc caacggcgag atggccaaga tggcgcgctg
     3961 ggtcatggag gaaaccgacc atgagctggt ggacctggcc tttaccggca tcacctatcc
     4021 aagactggaa aaagtggtgc agcgccagca tctgctgggc atgacgcagg tggtggtgtt
     4081 gccgtattac ctgttcaacg gcacgttggt ggagcgcatc aagcggcagg tcgacaacct
     4141 gaaaacccag taccccacca tccgcttcac cttaaccgcc tatttcggct ttgaacgcga
     4201 gatttttgag ctgctggaag agcgggttaa cgacctgaaa cgcggcgtgc ccgagtcacg
     4261 catgccgtgc gatggctgca agtaccgtga gtttggcgtc gaaaacggca tggggggcca
     4321 ccaccacgac gatgcgatgg cgcaccacca cgagcatgag catggtcacg accatcctca
     4381 cgaacatgcg cacgcacacc cgcatgttca ggccatcgtt caggttcatc cgcaccctca
     4441 ttcgcatcaa gatgcccatg aaaaggcgca tgaccatgac catcctcacg agcacgccca
     4501 cccatgagca ccgccaacgt cattaccgaa cagctcaccg ctgcgggccg ggccattgag
     4561 catgattcgt ttgcggtgat tgaccgcgag gtcgcatcgc atgcctatac accggcccaa
     4621 tggcccatcg tgcgccgcat gatccacgcc aacgccgact tcgacttcaa cggcctgacc
     4681 gactttcacc ctcgggcggt ggaggccggc gtggccgccg tcttgtcgcg cgcgacccgc
     4741 gtggtggccg atgtggaaat gatctgcgtg ggcttgtcgg cgccacgcct ggggcatttc
     4801 ggcatggcca cgcaccagtt catctcggac gctgacgtga ttgacgccgc caagaccgaa
     4861 gggaccaccc gtgccgtgca ggccatgcgc aaggctcacc ggctgggcct gatcaatggt
     4921 gccatcgtcg gcatcggcaa cgcgcccacg gcgctgatcg agctggtgcg cctgatccac
     4981 gaagagggcg cacgcccggc gctggtggtg ggcatgccgg tgggttttgt ctcagccgcc
     5041 gaatccaagg acttgatggc cctggaagcc gaggtgccct gggtcgtgat ccgtgggcgc
     5101 aagggcggct ccaccctggt ggtggcagcc atccatgcct tgctcggttt ggctgaggcg
     5161 agggaactcg ccccatgatg aaaaaagacg cgccccgcgg cacccgcacc ggtttcacca
     5221 ccggtgcgtg ttctgctgcg gcggcgcgtg cggcggtgat tggcctggtc acaggtcagg
     5281 tgcccgacgc ggtggaatgt ctgctgccca atggcgacct tgtgaccttc gccgtgcagg
     5341 atggccgcgt agatggcgtg ggtgggggcg catcggccca cgccatggtc atcaaggatg
     5401 cgggcgacga ccccgactgc accgacaagg cgcatctgac ggccgatgtg cggctgctgc
     5461 ctgagcggcc gggcgaggtg ctgctgctcg gtggctcggg tgtgggcacc gtcaccctgc
     5521 cgggcttggg cctggcggtg ggtggcccgg ccatcaatcc ggtgccccga cgcaacatcg
     5581 aggccaatgt gcgcgccgcc ggtgcatcgc tgctcgacga ggccggtttc gaggtgacga
     5641 tctcggtgcc ccagggcgag cagatggcca agaaaaccac gaacgcgcgg ctgggcatcc
     5701 tgggcggcat atccatcctg ggcaccactg gcattgtcaa accgtactcc accgcggcct
     5761 ggcgcgccag cgtggtgcag ggcgtgcatg tggcgggcac gctgcccgag ggcgtcgtgg
     5821 tgctcaccac cggcgggcgc accgaaaaat tcgtgatgga agaactgccc cacctgcccg
     5881 aggccgcatt cgtgcagatg ggcgactttt tacgctatgc catgggtgcg gcggtgaaag
     5941 cgggcttgaa gaaggtggtg atcggcggca tggtgggcaa gctcaccaag atcgcgcagg
     6001 gcgaaaccat cacccacgcg ggccgcgccg aggtcgatac cggcctgctg gccgacatcg
     6061 cggccggcct gggcgccgcg ccggacgtct gcgatgccat ccgccagaac gagaccgcgc
     6121 gctacgccgg cgagcgcatg gacgcgctgg ggctgggcca ggcgttccac accgcgctgg
     6181 cgcagcgcgt gatccagacc ttgcgcacgc gctaccccga tcaattcgaa ctgaaagtgc
     6241 tggtctgcga cttcgacggt cgcaagattg cggaggctcc ttgagcgata caaattttga
     6301 agaaaactcc atcgagcgtt gccgcattct cggcgtgctg gacgacggcg tcgccagcct
     6361 gggacacgcg gcgcgggcgc acctgcagca ggcgcagctg gtgatcggcg gggcgcggac
     6421 gctgcaattg ttcgcgcagc agatccaccc gcgtgcgcag cagcgcgacc tgaccggagc
     6481 cttatcccgg gtgcccgagt ggattcgagc cgcgcaggcc gcgggccagc gcgtggtggt
     6541 gctggccaca ggcgacccgc tgtgccacgg catcgccgcc tacctg
//
LOCUS       DAIDFG010000174         6581 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_174_length_6581_cov_17.869292, whole genome shotgun sequence.
ACCESSION   DAIDFG010000174 DAIDFG010000000
VERSION     DAIDFG010000174.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 6581)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6581)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6581
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174_length_6581_cov_17.869292"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1157 a         2106 c         2082 g         1236 t
ORIGIN      
        1 gagccggcgc catgtcgaga tcggcctggt gccgctgggc gccatcggca tgagtgtgtt
       61 tgcgatcgac ctgtatttcg cctcgcgtgg actgccgtct tcaccgatta tgggactggc
      121 cgcctttgtg gcccagccgg cgcactggcg cgtgttgctg gacctcacgc tgctgagcct
      181 gtttgcgggc ctgtacagcg tgcccatgta tgcgctgatc cagctgcgca gccagcctac
      241 ccaccgcgcc cgcatcattg cttccaacaa cattctcaat gcgctgttca tgatcgtcag
      301 cgccatcctc gcgggcgccc tgctcaaggc cgacttcagc attcctcagg tctttctgtt
      361 cgtcggcctg gccaatgcgg tggtggcgtt ctacatcttc atgctggtgc cggagtacct
      421 gctgcgtttc atcgcgctga tcgcctcgcg ttgtatttac cgcttcaagg tcacgggaga
      481 cgacaacatt ccggcgcagg gcgcggccgt gctgacctgc aaccatgtca gtttcattga
      541 cccggtgctg ttgatggcgg ccagcccgcg gcccatttat ttcgtgatgg accaccgcat
      601 tttcaaaatg ccggtgctcg gctggctgtt ccggctggcc aaggcgatac cgatcgcccc
      661 gcgtgccgaa gacccggcgg cgtatgaagc agctttcgag gcggcggcca aagtgctgcg
      721 caatggtgat ctgctggcga tctttcccga gggcggcatc acccaggacg gtgaactcca
      781 gcccttcaag gggggcatca tgaaaatcct ggaaaacgcc gaccgcgacg gcctcagcgt
      841 gcccgtcatt cccatggcct tgaccaacct ctggggctca ttcttcagcc gcattgagaa
      901 aggcggtgcc atggtgcggc ctttcaggcg cggcatcttc agccgtgtgg ggctcactgc
      961 cggccccgcc atgcccttgg cccaggcgca acctgaaatc ctgcgtgccc gcgttgccgc
     1021 actcatgaaa ccctgaaaag tatctcaatt caataccaac agggtattca aacgtcaccg
     1081 tattgttatt cgcccctgct tccggccatc ccggtgcaca ttggcgtcac atcaacaagc
     1141 aagaaggatt ttatgcaagt actgattcac cgcgattcca aaccctggca gttccaggtc
     1201 tgggcctctt tcctgattgc cgttttcctg tgcgccgtcg gcctgtcata cctgcccggc
     1261 caggacctgg accgcgcttt catgatcatg ggttactttt tctgcctgtc tgcggctttt
     1321 gtgctggcca agtatgtccg cgagtaggag aaggccgagg ccgaaagcaa gctgaccgaa
     1381 acgccaatgt tcaggtttgt ggtgtggggt ggctttttcc tggccatgtc gctcaccggc
     1441 tggggtttgt ggcgcatgga ggtcaacgag acctacaagg cttttctcgg cgtcagctgg
     1501 ctctacctga tcacctgcag cttcacgctg gccaaaaccc tgcgtgacaa acatgaagcc
     1561 gacctgagcg aagcgcgtct ggccggccgc gccacggtgc gagacaccga cattgaataa
     1621 acctgatcca ggacatgcaa ggaagtatca tgaaaaagaa atttgctact tatttaatag
     1681 ctgtttgcgc aatgacgacg ggggctacgg cctcttttca tgtccaggcg caaaccggcg
     1741 catcggccct gagcgcggtg tctgccttgc cggtggcgtc cgtggtggtc ggcaccagcg
     1801 cggttgcggg cgccgtcgtg gcgataccgg tggtgctatc gaccaccggc gcggtgctgg
     1861 tcgtcaagac cgtggagagc accgcccgcg ggacggtgta tgtgctggag cgggcctccg
     1921 acggtgcgcg ggccagcatt gaagtggtgg gcaagggtgt ggctggtgcg tccatggtgg
     1981 ccggaacggc cgtggttgtg agcgtgatcg gtaccggcgt ggtgctgtcc gcggccggcg
     2041 aggcgattgc cttcattccc aatgccctgg gccgcgccct gctgcacaac gaacggctga
     2101 cgcaatgaga aaacgcgagg gaacacccat gaaaaaagct tctctgactt ttctgtgcct
     2161 cggcctcacc gccgcttgca tgctggcgcc agcagcccat gcgggacgct cgtgcgaggc
     2221 cagcaagatc accccgcaga cggtggagcg cggcatgtcg ctggccgaaa aaaccctggc
     2281 ctcgctcaat gccagcggcg agaaggtcgt gctgctggca cgcgccggtc aggacctgag
     2341 caaatacaag ctgcgttatt cccacctggg gttggcttac caggagcctg acgggcaagg
     2401 cggccacacc tggcgtgtgc tgcacaaact caacgaatgc ggcaccagtg aatcggcggt
     2461 ttaccgccag ggcctgggcg agtttttcct ggatgacctg tggcgctttg aagcggcctg
     2521 ggtggcgcct gtgcccgagg tgcaggccaa gctgctggcg ttgctgcagg atcctgcgcg
     2581 caccgtgagc atgcaccaca agccctacag catcgtcagc tatgtctggg gccgcacata
     2641 ccagcagtcc aaccagtggg ccatcgaaac gctggccgcc gctgtggagc ccggcgccaa
     2701 ctcgcgcgag cgggcccagg cctggctgca gttcaagggt tacgagccca ccgtcctcaa
     2761 gctgggcccg ctgacgcggc tgggtggccg catgaccgcg gccaatgtgg cgtttgacga
     2821 ccatcccaac gacaaacgtt attcggaccg catcgaaacc gtcacggtgg attcggtctt
     2881 tgactggctg cagcgtgcac agctgacagc gaccggcaag tcgccgacct tcgtcaaact
     2941 ttaacaatgg ctgtttaact cccggagttt ccatgagcaa agcattacga ctgtccgaaa
     3001 aatggtttca gcgcggccta tggctggttg cctttgtgtt tgccgccttc ctcatcgggc
     3061 tgggcagcac ggtcgtgggc gacctgccgc aggtcgagaa aaccctgacc ctggacgatt
     3121 tcatcgagca gcctgccgcc atgcaggcgc gcgagagcct caggacggcc gaacgccagg
     3181 ggcggcaggc gcaggaagcg ctggaccagg ccaggctcaa gctgcgcgtg gcgcaggcca
     3241 attcgcgctc tgccagcgag accttcaaca actggctggc gacacggcgt gcgaccgagc
     3301 gggccgaaca ggaccccgag ctgatcgaac gaacacgcgc gctggacagc ctgaaggcgg
     3361 ccgagcggca ggcgctggcc acgatggagg ccgagcagca gaaggcactg gacgccagcc
     3421 agggcgccac gcgcacgcgt gaacgcatcg ccgagctgga gcgcgcggcg caggacaagt
     3481 ggcaaagcgc gcggcgcgcc caggagctgc gcgtgttttt gtaccggctg gccctgacgc
     3541 tgccgctgct ggtggcggcc ggctggctgt ttgccaaaaa acgcaagagt acctactggc
     3601 cttttgtctg gggcttcatc ttttttgcgc tgttcgcctt ttttgtcgaa ctcgtgccct
     3661 acctgcccag ttacggcggt tatgtacgct acatcgtcgg catcctcatc acggtgctgg
     3721 tcgggcggca ggcgattgtt tcgctgaacc gctacctgga aaagcagaag ctcgccgaac
     3781 agctgcccga tgcgcagcgc cgagaagagc tgagctacga cacggcgctc acccgcctgt
     3841 ccaagggggt gtgccccggt tgcgagcggg cggtggacct gaaaaatccg gagatcgact
     3901 tctgtccgca ctgcggcatt gggctgttcg accattgcgc gcagtgcacc gcgcgcaaga
     3961 gcgcgtttgc caagttctgc catgcctgcg gcgcggacgg caagcgggcc ggcgtgcttt
     4021 ccgcgcccgc gccgggcgct gcagcgcagg caggctccac gcccatgggt tgacggcgtc
     4081 ttgtccttgg cgcttcccgc tggccgccta cggccggcga cccccggcgc ctacaggttg
     4141 tgcgttgcgc tgccatcaca gtgggcaaac ccatttacaa ggtttgaaca tcatgaaaaa
     4201 acagatacgc aaccaggtcg cagccttttt cctgctgctt cccgccgcca ccgccctgct
     4261 cgccctgccg ggtactgcca tggcccaacc ggccgcgccc gagttgcgat cactgcaggt
     4321 gggtaccgac ggaggtctcc aggccggcgc caagctgcag ttcacgctgg aaggcacacc
     4381 gcggggtcag gtccgcctca ccgtgcgcgg tgcgccgcgc accatcgtgc tcaaggaaac
     4441 cgcacggggg atctacacgg gaagctacac catccggcgc caggaccgga tcaccgaaag
     4501 cagcgccatc cgcgccacgc tgaaagtgcg caaccgcagc atcgcaacca actacacctt
     4561 tcctgccggc atcgccagcc aggacgtggc cacggcgccg gccggcctga aaatcgaccg
     4621 cttctcggtg gctcccgtcg acagaatcga accgggcgct gaactgcgct tcacgctgaa
     4681 cggcgcacct ggcggtacgg cggagttcga cattcccggt gtcatcaaca atgttgcgat
     4741 gcgcgaagtg cggcccggtg tttatgaggg cgcctacacc atccgccgcc tcgacaacct
     4801 ggcgccgtca cggcctgtcg tcgccaccct gcgtgtcggc gaccgcgctg tcacctcgac
     4861 cctgacccag cccctgatgg ccgatgcgaa gccgccggtg atccgcaaca tgtccccgcg
     4921 cgatggcgag tcggtcacgc gcggtgcggc cacctcggtg tccggtacct ttgacgatgc
     4981 gggcggcgtg ggtgtggacc ccaagagcgt tcgcatcatg ctggcggggc gcgatgtcac
     5041 cgccgccaca cagatcacgc cgcagttttt cacctatcgc gcggacctgc ccgccggccg
     5101 ctacgcggtg gacgtgacag ccaaagacct ggtcggcaat gccgtgcgcc agacctggac
     5161 cttcgacgta gcttcggccg tgagcgccgc gcctgccacc gtgctgttgc aggtaaccag
     5221 ccacgccaac aacgccgtgg ttgaaggcag cccggtggtg gtgcgcggcc gcaccgcgcc
     5281 aggcgcggtg gttgatgtca aggtcaacgc cgtcggaacg ctcgccggcc tatttggcat
     5341 gaaccaggat ttgctgacgc agcggattca ggccgatggc aacggcaact tctcgttcag
     5401 cttcacgccg cagttgccgc tgcccggcac gcgttacgag gtgtcgatga cagctggcaa
     5461 ggacggcatg gtcgccaccg aaaccaagct ggtgctgttc cagaagcagg gttaagccgg
     5521 atttgcagta aaaactgcct gcagccctta tgtagcgggc gcaggcagct actgattaaa
     5581 tagcaacggg ctgaagcggg cggccgtaaa aaggataact cgggtcgttg tagccgtggc
     5641 tggaggcgtg gcccggaatc accagctcgt tgatgaaggc ctcgtcctca gcgtccagca
     5701 ctaactcggt cgcgccgtag acgtcttcca ggtgcgagag caggcggggc ccggcaatca
     5761 ccgaactgac cagcggattg gccagcaccc aggcggcggc aaactggccc ggcacccagc
     5821 ctttggcctc gcagcgcgcc ttgatcttct gggcaatcac cagcgactct tcacgaaact
     5881 cggtttccag gatccgtttg tcgccgctgc cggcgcgtgt gccctcggcc ggtggttggc
     5941 ccggcgggta cttgccggtg agcacgccgc gcgccaacgg gctgtaaggc acgacgccca
     6001 acccgtagtg gccgcaggcc ggcaggattt ccacctcggg gccgcggttg agcaggttgt
     6061 aatacggctg gcagaccact ggttgcggca cgccggtttt ttcgcagagg cgcatgatct
     6121 cggcaatgcg ccagccgcgg aagttggaca ggccaaaact gcgaattttt ccggcgcgaa
     6181 tcaggtcacc cagcgcgcga accgcttcct cgaggttttc gccgtggaag tcgcggtgca
     6241 ggtacaggat atcgaggtag tcggtgccca gccgcgccag gatggcatcg gtctgctgca
     6301 tgatccagct gcgcgaatag tggccctggt tcacgcccgg cccggccttg ttgcccagct
     6361 tgctggccag cacccagtgc gggcgttgcc cggccagcag tttgcccagc aggatttcgg
     6421 acttgccttc gttgtagacg tcggccgtgt cgatgaaatt gatgccgcgt tccttcgccg
     6481 aagcgacgat gcgcgccgcc tcgccgtcgg gcgtctgttc accaaacatc atggtgccca
     6541 ggcacaggac ggacaccttc aggttgctgt ttccaagccg g
//
LOCUS       DAIDFG010000175         6581 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_175_length_6581_cov_16.995403, whole genome shotgun sequence.
ACCESSION   DAIDFG010000175 DAIDFG010000000
VERSION     DAIDFG010000175.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 6581)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6581)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6581
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175_length_6581_cov_16.995403"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1080 a         2001 c         2380 g         1120 t
ORIGIN      
        1 gtgatgttgt gcagcacccc gggcacgccg gaagacttgg cgttgtaacg ctgtagcggc
       61 aggttgttgc tgccgttggg cacctcggaa aattcgcggg ccatgacgct ggagccatcc
      121 ctgtggaccc agctcgcgcg tgcccccggc ttgtgcatga tggcggcgcc gtaggtgtcg
      181 gtcaggtcca cgctggcgtt tctcatcagc cactcgtatt catacggggc gcgtctgacg
      241 gcctggcatt cgtacagcag cctgccgttg ctggtggctt ccagcacggc ttgatacccc
      301 tcgggcaccc gaacggcctc gggcaggtct tgctgggtgt tgtagtgctg cagcggccgg
      361 tagccgcagc ctcccagcaa ggcggcactg ccaataagca caaacaggct gaggctgcgc
      421 gacacgcggc caacggggaa attgcggcgg gagggtgttt tcacgaagat tccggagtgt
      481 gagcttgttc tagtacaccc catgtttatg gggggtgctg tacgggaaaa cccccgcgtt
      541 tcagcgcaat ctgtcctgtc cgggaccggc gcagggtgct tttcagccca gggtgctgca
      601 ggaaatgtca tacgcggcca gggttttgag tttggagccc tggccgggca cggcgttgtt
      661 gcagcttttc agggtcttgc tgaagccgcc cggtttggct tgcaggctgt aactcacacg
      721 gctgaagcca ggcgcatcca gggcgagggt ggggcgcatg gccacggcgg tgctgccgcg
      781 gttttcctgg gccagggtgg tttggggggc cggcgtcagt tcccacgcct gggttccgtc
      841 ggcgcgagcc acgcgctggt acagcaggtg gtcgtcgccc gtcagtggaa gggcttgcgc
      901 ggcatcaggc aggggagcga gcaccaccgg ctggtcatag gcgccaaagc cgcggggaga
      961 ggagaccggt cctgaaaaaa ccgcgatcat ggccgccgtg ccggtgacca ccagcaaggt
     1021 caggccgatg gccaggctga tcgcgcgaaa cagatggtgc gtgtttctca tttcaggtta
     1081 ctccttgctg atcatcagac cgcagattcg ggccgggtgc tgtaggaagc cgccccttgc
     1141 gtgagacggc gcacagcgcg gcgtggaacc acggtctccc atgcattgca gctttcccgg
     1201 cgggctggtg gggaggcgtc tcatgggagg tctggttcat tattgggtgg ggctgaggac
     1261 cagtttcagc tgcaacaggt gtaagcgtct gtaggacgct gcgcccaggc cctgcgggcc
     1321 aaaaacgcga tttgccgcat gcaccaaaaa agcctgcaga ccaccactca cgcgatggcc
     1381 tgcaggctgt caaagccggc ccccgccaga cgcggaaacc ggtcgctcac catttcggct
     1441 aacttcccaa aaaaacggca agtgcggggc ttacatggcc ttgtagaaga tgtagtcggc
     1501 ctggtacttg acgatctgcc tggtgcccag gttgccggcg gtgcaaggtg tggccggtgc
     1561 cacgccgccc acggtggcca cgcgctggat gtaggtgacg ccctgcatgg caccgctgcc
     1621 cgtggcgggg ttgcccttga cgagctgcag cgggatgttg ccggccatgt tgggcgcgac
     1681 ggcgagctgc gtggcagtca gtttggagcc gtccatggcc tcccaggtgg cgggcgggcc
     1741 gtagtacttg ccgacctgtt tgccgccgcg gtccatcagt ttggcgtcgg gaccgacaaa
     1801 cacccattcg aactggccga ccatgtcttt cctggcgctg cactggtagc tgatgtcgcc
     1861 catgcccacg gtttcgaggg ctacccggtt gccggcgggc acctgcacgg cggcgggcag
     1921 gctggcctgc gagtaggtcg gcatcatagt gttcatgctg ccgcaggcgg caacaaggga
     1981 gacggcggcg gtggcgatgg cggtacggat catcatgagg ggcactcctg gtagtgagct
     2041 ggcgggcgcc agttgctggt tgtggatgtc gcggggtcaa cgcaccggac ggccgcaatg
     2101 aatgaagccg gccgttcgat ggcgtctgcc gggaatacgt ggccggcgcg ggtgtggatt
     2161 cagcgcgggc aggtcgggcg tgaaaaaaca cccgccgggc tgtgcggggc gagaggcgga
     2221 cgggtggtgc tggcgggcgc ggtgccctat tgcccgaagc gcgggccgat tttcaggaac
     2281 tcgaattcca gcgcgctgct ggtgcgcccc agcgcggtat tgcggtgggg aaaacggccg
     2341 tagcgggcaa cgatgtcgcg gtgcttgaca gcaaagtcca gcgagccctg ggcgctggcg
     2401 cggtgcgcct cgggcacggc cgccaacagg gcgctgaagc ggcgcacgcc ttcttcctgc
     2461 agggccaggt tttcggcatg ggtgagcggc atgtacagaa agacacggcc tatccagggc
     2521 agggcctcgt cgatgccttc cgcgagcgca tcgctgacca gccgctgggc ctgctcgtcc
     2581 ccggcaaagg cctggccctt gccgcgaaag atatggcgcg gaaactggtc gagcaggatc
     2641 accagcgcca gccggctcag cggctgcgcc tcccagccgg cgagaccgcc ggccagcgcc
     2701 tgttcgacca gcttgccaaa ggtctggcgg atttcctcgt cgagttcggg cccgccattg
     2761 aaccagcgct tgcccatgtc ctggctgggc cagcccagct tgaggccatc gccgaaccag
     2821 aaatcgagga ccagctcggg cgaggtggaa atcggaagcg gcgaggaaga cgtggaggac
     2881 gttgaagagg gcatgggcgg caggtacggg gcgggcaggg acggggtgtt tacatcgccc
     2941 tgaactgctg actgtacatg cggctgactg atagtttttc cgtcctgccg cgcaggctca
     3001 aggagagttt gccggtctcg tcgcggctga ctgcttcgat cgcgccggcc tgcaccacgg
     3061 tgccgcggtg gatctgccag aagttttgcg tgtccagctg cggcaacagg tctttcagcg
     3121 gtgtgcgtat caggtactcg tgatcggcgg tgagcacccg cacgtatttg tcggccgctt
     3181 caaaataaac cacctcgccc accggcacca tgcggatggt cgtgcccacg ctgatttgca
     3241 gcaccttcag cgggccgctc tcgggcccgg gcgcccgggt ggcggccagc agctggcgca
     3301 actggtccag ggtcgtgtcc aggggtgctc ctgaattcat agctgcttgc gcccgtgtgg
     3361 aaagggcttg ttgcactttt gatactgttt tctgcaggcg cccggcttgc acgggcttga
     3421 gcaggtagtc caccgcctgc gcttcaaagg cctgcaccgc gtactggtcg taggcggtga
     3481 caaacaccag cgcgggaaac ggcgtgtcct gcggccagtc gtcggccagt tcggccgcgg
     3541 cctccaggcc gcccaggccg ggcatgcgga tgtcgaaaaa cagcacctcc ggcagcagct
     3601 gcagcgactg cgccacggcc gaggcgccgt cacccaccgt ggccagcacg cgcagcgcgg
     3661 gccaggcgcg tgccagttcg gcctgcaggg cctgggccag cagcggttcg tcttcggcga
     3721 tcagggccgt ggccgggtgg tcggggtggt tgttcatggt ttgaagggaa atcggacgct
     3781 ggcccttgta ccgcctgcgt cagcagctac caattcgata gcaatttcgg cgccgtaggt
     3841 gctggccagc cgctcgcgca cctgggccat gccgaaaccc gcgtggtctt tgtcggcacc
     3901 cgacaggccg acgccggtgt ccagcacctc cagcaccagc gccgcgccct cgcgccgggc
     3961 gcggacggtg atgcgaccgc cttcgacctt gggctccagc ccgtgcttga tgctgttttc
     4021 caccagcggc tgcagcagca gcgtgggcac gggatgctgc gccagttcgg cgggcagctc
     4081 cagcgtgtag gacaggcgtg gccccatgcg gatggccatg agctcgaggt agtcgcgcag
     4141 gcggtcgaac tcggcctgca gcgtgtgggt gggtgacagc gaggcgccga gtgtggcgcg
     4201 caggtagtca atcatgtggt cgagcatgac ctgggcgcgc ggcgggtcgg tggcaatcag
     4261 cgcgcgcagg ttggccaggg tgttgaacag catgtgcggc tcgagctgcg tttccagcag
     4321 cttgaggcgg gcctcgctgg cgtgctggcg cgcctcgtcc atcttgcgga tgaggtaggc
     4381 gcttttgctg atggagtaga agtaaaagct gccggcaatg ccggccagcg cggtgatcag
     4441 gatggagttg cgcaactggg ttgggtcctg gtggccggcg ccgggcgcgt accagccgta
     4501 atagtcacac agccggtcgg cgatggtcgt gcccagcagg tagcccagca cgatgctggc
     4561 tgtcacaagc accaggccgg tccagccctt gggccacccg gtctcccggc tggaagggaa
     4621 caggtggcgg ccgatgtcga tgccggccca ggtgatgccc gcaatcagca gcgaatacac
     4681 cagcggcggg ccccagggcc gctccggcat gaaggcgtac tggatggtgg cgatcaccag
     4741 cgcgaaggcc gttacctgca gcaggtgccg cagcttggag gtccagtcga tgttcatcgc
     4801 tgcgggccgt cgccgtgctg gcgctgaagg cgttcacgct ccttctgcac catctgctcg
     4861 cgcaggccgc tgccattgcc cagcacgaac accgacacgc catgcagtgc cagccccagg
     4921 ccccagccca gcaccgggaa ccatgaccag ggccggctgc ccaggccgta ggtcgacatg
     4981 gcgaagatca ccgcgttgat cagcaggtag agggcagcgt ggacatacca gcccagcttg
     5041 gcgcccgcgc gcttgtgggc caggcgttcg atgtcttcgg gcaggggcgg ggtatgcatc
     5101 atgggaaggc gtgaaaaaaa cattctaggg cagggcaggc ttcaggtgcg gcaggcctcg
     5161 atgatggccg gccaggcgcg cgggtccagc gtcaggccca tgtggttgac gccgggcacg
     5221 agggtgaccg gcacacgctg gccggccgcg gcaaacaccc cggcgaactg ttcggcgcga
     5281 aagagctcgt cgtcgttgcc caccacgatg cgcagcgcag tgcggatgcc gcggatgtcg
     5341 gccaggtagt cgtcatgcgg gccgaaactg gtggcgaggg tgaaggagta cgagggcgtg
     5401 agcaggggcc gggccgcgtc gttcaggccg aagttcagca ccggcagctc attccaggcg
     5461 gtcaggtcgg cgcgattgag aacatgcagc gcgaccaggc ggggcaggcc caccgacacc
     5521 cagccgccgt tgcccggccg ggcggtgggg gcgtcgtggc gcaggtaggg cgagagcagc
     5581 acgtagcggt caaacagggc ctgccgcgcg ctgccggcaa aacgcagcac aaagccgccg
     5641 ccggaggaaa aacccagcag ggtgctgggg cctgcatgcg gcacggcacg catgaagtct
     5701 tccacgtcgt cctcgagctg gcccacgtag gcggcctgac cgcgcgggcc cgagtcgccg
     5761 tggccgcgca tgtcgagtgc ggccacattg aagccggccg cagccagcgc ctgggccagc
     5821 gggtgcatgg agcgtccgcg cgccgacgag ccatgcacca gcacgatgcg gcatttttcc
     5881 aggctcccag gggcggcagg gtagtcgagc caggccaggg acgtgccgtc gcgggccaca
     5941 taagaccgtg ccggcggcac ggcgctgaag tccaccttcg caaagggttc gttgatgctg
     6001 gccagcggcg cgatgggtgc cggcccgccg aaggcaaccg cggcggcgag tgccgtgaac
     6061 agcaggacca cggcggcgaa ggcggcgaaa gtgatggatc gggtcatggc agcaggtggt
     6121 ggcggaaaaa ggcggtgatc ttctgatcga cctcgggcag ctggctgcgg tcgaagcccg
     6181 gcgggtcgcc ccgccaaagc gcgcaggttg gctggtattt tttcatggtg tgctgactgt
     6241 cattgcgtgc tgccggttca ccagggatcg cgctgaagcg acaggcgttc acgttcccgc
     6301 tgtaccaggc gttcgtgcag gccggcgccg ccggtgagca ggaacaccac ggcgccatgc
     6361 acggccaggc cgatgcccca gccaaaggcc gggaacaccg cccagtggcg gccgctcagc
     6421 gccgacaggg caaacagcac gaggttgacg gcgataaaaa cgctggcgtg gatgtaccag
     6481 cccattttgg cgctggcgcg cttgcgggcc aggcgttcga tgtttttgtc ttgcggggta
     6541 tgtggggtgt gcgggttcat ggtgagttcc ttcgttcagg c
//
LOCUS       DAIDFG010000176         6483 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_176_length_6483_cov_17.634256, whole genome shotgun sequence.
ACCESSION   DAIDFG010000176 DAIDFG010000000
VERSION     DAIDFG010000176.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 6483)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6483)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6483
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176_length_6483_cov_17.634256"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1129 a         2027 c         2058 g         1269 t
ORIGIN      
        1 catgccgcga tggatcccgg atcgagtccg ggatgacaat atctgtcact tttgctatgt
       61 aatcaatagc tgcttgcgcc cgcccactat gggctagagc ccgatttcct tgaaactctg
      121 gagcggtgac aggcctgcga acccgcctgc gcgtttttcc ttcatggccg tcacggcctc
      181 acgcacatgc tggttgctcc agatcgcgcc ctgcagccag cccatttgtt tgagcgcgtc
      241 gtccaccgaa tggtcacgcg tgtagtggat ggcctgcttg gtgccccaga tggccaccgg
      301 cggcttgctg gcaatttcct tcgcgcacaa cagcgcgccg gccagcaggg cttcgggcga
      361 atcgaacacc tcgttgacca ggccgtaacc cagcgccctg gctgcgccga gccggcgtcc
      421 ggtgtaggcc agctccttga ccacggccag cggcaccagc ttgggcaggc gctgcagcgt
      481 gccgacatcg gcgaccatgc cgatgttgat ttcctggatg cagaaaaatg catcgcttgt
      541 cgcgtaacgc aggcagcagg cggtcaccat gtccaccgcg ccgcctatgc agccgccctg
      601 gatggcggcg atcaccggga tgcgcagggt ctcgagtttg gtgaaggtgg cctgcatgtc
      661 ggccagcatg tcgaaaatgg cggcgcggcc ttcggggctc tggtcgtcca tcgcaatggc
      721 gccgctgaag gtctccagcg ccatgccggc gctgaaatgt ttgccggtgc tggagatcac
      781 cagcacacgc gcatcgccgg ccttgtggat gtgggtgagc acctcatcga gctcgcgcca
      841 gaaggtcggg tgcatggtgt tcatcgcctc ggggcgattg agcaccaggt gggcgatgtg
      901 gtcggtggtg gaaagggtga agcaggtcag tgggttcatg cctgtcactg taattcagtg
      961 caccagttcc agccagtcac acctgagacg gccgcgggta tgggcgcgcg cccgccagcg
     1021 cacatacagc tgcggcgcaa gcatgccgag caggcacaag gggtaggcca gcgggatgtc
     1081 cagcgcaaag gccggcaacc agccgaccca gcccatgacc cacagcgtca gcacggcgtc
     1141 ccagagctgg tgctccagcg gatggctgcc ttgatggtcc aggtgccact gcttgatgcg
     1201 ctgcagcccc gccagggtag ggtgccagct ttccggggaa gttgaagggt gcgccataaa
     1261 gcctccgtgc agaggcttca atgtaagttt caagaaagct tgtgtcaacg cccccggtct
     1321 gttgcggccc ggcggggtga acaggcccta cttggcagcc gcgatggctt tggccaggtc
     1381 tgtggtttct tcacagctgc cgcagatggc ctgcagcttg gcaagctggg cttcggcctg
     1441 ggctttctgg ccggtcttca agtaggcaat gcccgagtat tccagcgcgc cgcggtgttt
     1501 cggatccagc gtcagcgcct tgttgtaggc ggcaaaggcc tcgtcgtatt tgcccagcca
     1561 gcggttcgaa taaccgaggt agttgtaggc gtcggcgttt ttcgggttct gcgcgacgac
     1621 tttgctgaag ctgctggcag cctcggccca ttttttggct tccagggctt ttttgccggt
     1681 ggcaaagtcg tcaaggcgtg cgttttccac aggggtggcc tggaagtcgg cggccagtgc
     1741 agcgccggcc aggacgaagc ccaggatggg cagggcgagg aattggcgaa gggtcagtgt
     1801 gttcatgtcg ggctcctttg aaatatcggt tgttgacgtg cgctgtgatt cagcacctag
     1861 gacaaacgag ggaaaccgcg aaacggatgc aaggcggatt gaaattattt gtcatcgggc
     1921 cacggcaatc tgcaaacgag gaaacggtca caagcgttcc aggcttcgaa tccgtacttt
     1981 tcagttcacc acaatttcgc gcaattgccg ctgaacacca cgggtccact cggggcggca
     2041 actgccgcat tcgtttccag cgtcaccacc agcttgccga ccttggacag cagtttctcg
     2101 gaggtgtcgg gaagcatgga taccgccgag ccgctggtgg gcacggaccc caatgcaaag
     2161 gccggaccgc tggccggcac cgcccacagc accagcgtgc ccccagccgg cggcgtgatc
     2221 gggccgatga ccttgaccgt catggttctg ccgtggcgca gcgagctgac cagcagcttg
     2281 ccgttgccct gggcatcggt cagcaggccc acataactct gcggcagttt ttcgccggtg
     2341 cgcatggcga tctggtcgct ggacatgaag aggcccggtg ccatcacaaa caaaacgctg
     2401 gcagccatca caccggcggc gaggccacct aaaccaaagc ccgcgggttt gagccagccc
     2461 agccagccgg ctttggcctg cgctgcgtgt ggcggcctgg ccgtttgcgt gcgcgccgcg
     2521 atcgcggtcc acaccctggg cgagggttgg tgcaccggca ccgacacagc gagctggccc
     2581 agccgcgact cccactgcgt gacgcgcacg cgcacatcgg cgcggtcgcg ctgcaggcgt
     2641 tcaaagcggc ggcgcgcgcc gcctgtcagc gtgccgagta catacgatga cgccaggtgt
     2701 tcgagcaggt cggggctggt atatctcatg ccaccacccc ctgcgcggcc aggcagccct
     2761 tgagcttttc gagcccgcgc cgtatccagg ctttcaccga gcccagcggc gcgcccattt
     2821 gcgcggccac ttcggtatgg ctcaagccct ggtaatacgc cagggccagg ctttgccggt
     2881 agctgccgtc gagcgcactc atgcagcctt cgatgtgcag ggcctggctg gcctgttcaa
     2941 gcaagtgcat ggcgctgggc accgggttgc caacggactg gacatcgtct tcatcgacgt
     3001 cgcccgactc gggcagctcg ctttctcggc ggcgtgtgcg gacgcgcaag gcgtccagcg
     3061 ccttgttgcg cacgatggcg atcagccatg tcatggcttg aatggtttgc ccgcctacct
     3121 cgctgcggta gctgccggca cttttccaga tactcacata cgcctcttgc agcacatctt
     3181 cggccgcctg tccttgaccc aatatacgaa cggcaacccc gaacagatgc aggtgcgtcc
     3241 gttcatagac ttcggcgaag caggcgtggt cgccctgcgc cgcagctgca atcaacgctt
     3301 ccaggctggg ggtgtccggt ttcatgcggc tgagtatagg gagttctgcc atggcggcaa
     3361 gcacccggtt tggcgaaaaa aagtctggat tcctgcatcc ggttggtcaa acgcaacgtt
     3421 tgtcacggat aggcagggcg gccacatgtc atggcgccga gccacatttc cggtcggccg
     3481 gcttgtccgt ctgcacattc acttttttca ggagattgtc atgacatctg ttctcgcaac
     3541 accgctttcc ggctccggca tggttctggc ggtgcattgt gcgggcttgc tcggcgcttg
     3601 cgcttcccca cgggctgggc ccgcagaggc acgcccgccc gtgtccgccg cgctcggcgt
     3661 caccagcgaa cagcgcctcc acaccgaagg cggcaatgcc caagcgcgcg gctgcacagc
     3721 tcaggctgac gcgggcaagc gcatcaagga aggctgcgcc gcagactttg ttttcttcac
     3781 cgccactttc ccctgatatc ggtatgtctg cactcaaaaa tttattcacc ccgggaaagc
     3841 gttcaaagtc cgcacccgcc gggccggaga ctgcgcctgt caagatgaac cttgaggagc
     3901 gcatggagtt ccggcgcgag atgttgtatg aagccatcag gatcaccatg caggcccacg
     3961 gcatcctgtc ggcaagctac cgcttccgcg tggtgcgcaa cgacaggcgc ggccacatgt
     4021 actccgtgat gatggatctg tccaccgact tcatgcacaa ccgcaagggc tgtcccgcgc
     4081 aactggtcgc cctgggcatg gccatcggca aaaatgcagc cgcgcgttat ggcatcctgg
     4141 tgagcggcgt gtactggcga gtcaacgacc agatacaggg gttcgagccc agccggccgg
     4201 acgctgagtt cggtgccgac gccacggcgc cggccctgcc gccgccgcgc ccccggcatg
     4261 agcgtgcagg ggccgaggag ttggcggcct tcgaggccgc ctggcagaaa gagaaagccc
     4321 aggagttgcg ccttcgtgac cgcgtttatg cgtccgatct tgcgccgctg ggggatctgg
     4381 cagaaggcgt tagccggcgt taacaggggg ctaaatccgg cgccgtcgcg gcaccacgcc
     4441 cagttgcagg gtggtcttgc cgggtgcggc gctcacagtg gtttgctgcg gcgcggtgtc
     4501 cggcggctga cggtttgcgc ttgcaaaaaa gctatcggca gctggcctgc acctgtttga
     4561 agtccagatg cccttgcagg atcttttcaa tcgcatcctg ctccagcgtc gtcatgccgc
     4621 cttccgtcgc ttcatgcagg atctccgcgg acgttgcccg tgtctggatt ctggccttga
     4681 tgactggtga ggcgacgagc agctcgtaca cccccatgcg tcccttgtag ccgctatggt
     4741 cacaggtcgg acatccgacc gccgaataaa gcgtcagcgt tccatcgctg cctccataac
     4801 gtgagcgcca gcgaccggcg acatcggatg ggttccgctt cacatcgcgg cagtattcat
     4861 gcgcgagcgc gtcgatttcc tcgggtgcag caagatgcgg cctgcggcag gtcgtgcaca
     4921 ggcggcgcac gaggcgctgc cctacaacgc ccagcagcgc atcggagaag ttgaaggggt
     4981 cgagtccgaa atccagcagc cgcacgatgc tttcgacagc gctgttggtg tgcatcgtcg
     5041 aaagcacgag gtggcccgtg agcgaagcgg cgatcaccgt gcgggccgtc tccggatcac
     5101 gcgtctctcc caccatgatc acgtccggat cggcccgcag gaagctgcgc agcacctcgg
     5161 gaaaggtcag gccgagcttc gggttgacct ggacttggca cagaccgtcc tggctgatct
     5221 cgatcggatc ctcgaccgtc cagatcttgc gctggggcgt attgatctgg ctgagcagcg
     5281 aatgcagcgt cgtggtcttg cccgacccgg tgggcccaca gacgaacagc aggccgaaag
     5341 acctgttcga gatcgccttc agctctgcaa ggtcgcgagg cgacagcgcg agctggtcca
     5401 tggacatggc gcgtggtgcc gcgaggatgc gcatgaccac gtcctccatg ccgttcgccg
     5461 tgggcatcat gatcacgcgc aactccatgg tccggggacc gaaatcctcg aagcggatcc
     5521 ggccgtcttg cggccgccta tgctccgaaa tgtcgagttc gctcatgatc ttgaggcgcg
     5581 agaccagtgc ggcgcggaag ttcgacggaa tgtcaatgta cggtgccagg acaccgtcga
     5641 cacggaaccg gacccggctc ggcaggccgc ctgcccgcga ttcgatatgg atgtccgagg
     5701 cgccctggtc ataggcgtcc atgatcatct tgttgaccag ccgcacgagc gtattgtccg
     5761 actcggtgac atcttcctcc ttggttcgct ccacggtcag gctctctttt ccaagcttca
     5821 cgaccagctg agcgatcttc tcgctatcga ttgcgctgtc ataaaactgc gcgatggccg
     5881 cctgcagatc ttctcgcgcc gccatgactg gatcgacctt gagtccggtg aaaaattcga
     5941 gctcgtgcag tgcctcccac gacaggggat tctcgatgcc taccgcgatt ctcttgtccg
     6001 tgcgatacag gggcatcacg aggtacttgc gcgcgaggtc gggggagacg gccttgatgg
     6061 cgttcgggtc gtactgaaac ctcgccaggt tcacagaggg cacaccgagc tggccgacca
     6121 gcaccttgcg gatcgcctcg cgggtcaccg ctcccatctc gacaagaatc tcgccgagca
     6181 gcttgtggcg gtcaacggcc tgttggccga gcgcggtatc gagctgctgc tgggtaacga
     6241 gtttgtcctg gatcagaacc tctccgagct tgcgacgcgg catggtggcc tgttgcctga
     6301 gggcgacttc aagatcctcg cgcgtcacaa agccggcctc ggcgaggtag tcgccgatct
     6361 tcttggtctt gagcaattgc tgcattcgca accctgcttc cagggcttct cctgaaagcg
     6421 catggttgtt gatcagagcc tgcccaaggg gctcttcgat gcggtaggtc gccaccagtt
     6481 cgg
//
LOCUS       DAIDFG010000177         6388 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_177_length_6388_cov_15.775146, whole genome shotgun sequence.
ACCESSION   DAIDFG010000177 DAIDFG010000000
VERSION     DAIDFG010000177.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6388)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177_length_6388_cov_15.775146"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1082 a         2077 c         2065 g         1164 t
ORIGIN      
        1 gccgagctgg ccgacatcat tcccggcgcc gaggcctggc cgcggttcac caccaaccgc
       61 agcgagcgtt tcgaagcccg tctgagcctg gtcgaagtgc tcgactcacc gagtctcttc
      121 ctgcagggca tggccggcag ccgcctgccg attgcggtgg cgcatggcga ggggtatgcc
      181 aactttgcct accgtggcga cgccgccaag gtgattgccg cgatgcgttt caccgaccac
      241 cacggccagc cgaccgaagc ctacccggcc aatcccaacg gcagtgccgg cggcctgacc
      301 gccgtaacca ccgccgacgg ccgcttcacc gccatgatgc cgcatcccga acgtgtgttc
      361 cgcaatgtgc agctgagctg gacggaccag gacatcagcg cgtccagccc gtggatggag
      421 atctggcgca atgccaggaa atgggtgaaa taggcctcca gccctttttg gatgtgcgct
      481 gactgctatt taaaagatag catctcagca tgccacccgg ggcttgcccc gggttggccc
      541 tgatgcgccg gggcgctgcg cgcggttcaa tgccgcatga acgcctctgc ccatcccccc
      601 gagtccacca ccaccgccgc ccttgccatg ccgccagcgc cgcaggttcg cgtactgcag
      661 ccgctgcgct tgggcgaccc cgtgcgctgg ctgcgcgccg gctggcgcga cctgagggcg
      721 gcgcgcggca ttgcgctgtt ttatggcatc tgtttctggc tcatggccgt ggtgctgggc
      781 gcggtgttcc gcaacctgcc gcagtacacc atgtcctttg tgtcgggctg ttttctggtc
      841 ggccccttcc tggccatggg gctgtacgag tccagccggc ggcgcgagca ggggctgccg
      901 caaagcctgg gcgcctcgct gacctgttgg gacagccacc tgggcagcat gggcatgctg
      961 gtgctggtgg tgatgttgct ggaactgctg tggggcaggg cctcgctggt cgtttttgcc
     1021 gtgtttttca acaccggcat gccgagcacg gccggcgtgc tgaaggcggt gttcaatccc
     1081 cagaactggc agttcatcgc ggtatacacc gtggtgggca gcgttttcgc cggactggtg
     1141 ttttccacct gcgtggtgtc catcccgatg atcctggacc gcgacaccga tgccatcacc
     1201 gccggcattg ccagcatcca ggtggtggcg gggcgcacag gacccatgct ggtctggggg
     1261 gcgctgatcg tgctgctggt cctgggcgcg atgctgttgc cctgggcgct gggtctgctg
     1321 gtggtcggcc cctggctggg ccacgcgagc tggcatgcct accgcagcag cttcgcctgg
     1381 gaggcgccag caccggccgt acccgcctga cggacgagta agcccggcca gggaaagcca
     1441 gcccggcgac aggtgccggt ggcgaaggtg tggcgcagcc ttcgtcaacc ggaaagcccc
     1501 gcgggtcgcc tgcttgccca gtgggcgcac cctccggttt tccgtcctgc gctcaggacc
     1561 gtgtcctaca cagcgcagcg gggcgcaatc ttagcgtgat tgttggcgat tttgcctcta
     1621 ccaacatcat caaaaccatg cccaaaacca atccgccggc cgacaaacgc aagccgggcg
     1681 cccaagccgc tgcacgcaac accgacactg gccccgcgca cttgccctcc gacaccggtg
     1741 ccgacctgcc cgcagagaag atggctcagg tggagtccct ggtggcggcc atgccgcaca
     1801 accccaccaa agctgccgag cacggctttg ccaacgccat cgccccccag cgcggggcca
     1861 cggtaaagcc cgagtcgagg ctgcccggcg gcagcaccct gaccgaggaa aacggctccg
     1921 aaaaaaccgg cagcgtcgcg ccggaaggcc tcaacgccac catcgagccg ctggaccgcg
     1981 tgcgcgttga ttcggccggc cgcgtcctca ccaccaacca gggggtggcc atcgccgaca
     2041 accagaactc cctcaagttc ggtttgcgtg gcccggccct gctggaagac tttgtgctgc
     2101 gggaaaaact cacccacttc gaccacgagc gcattcccga gcgcatcgtg catgcgcgcg
     2161 gctcggctgc gcacggtttt ttcgaagctt atgaaccgct gacgcgtttc accaaggcgg
     2221 cgccgttcaa ggaagccggc aagatcaccc cggtgttcgt gcgcttttcg accgtggccg
     2281 gcgagcgcgg ctccaaggac acggcgcgcg acgtgcgcgg ctttgccgtg aagttctaca
     2341 ccgacgaggg caactgggac ctggtcggca acaacatgcc ggtgtttttc atccaggatg
     2401 cgatgaagtt tcccgacctg gtgcacgccg tgaaacccga gccgcaccac cagatgccgc
     2461 aagccgccag tgcgcacgac accttctggg acttcgtctc tctgatgccc gagtccaccc
     2521 acatgctgat gtgggtgatg tccgaccgcg ccatcccgcg cagttacgcc accatgcagg
     2581 gtttcggggt acacagctac cgctttgtca acgaggcggg cgagtcggtg ttcgtcaagt
     2641 tccactggga gccgaaatac ggaacgcact ccctggtgtg ggacgaggcg gtgaagatct
     2701 cgggcgccga ccccgacttc caccgccgcg acctgtggga acgcatcgaa tccggcgctt
     2761 accccgaatg ggaactgggc gtgcagatct tcaccgaagc gcaggcggag gaattcagct
     2821 tcgacatcct ggatgccacc aagctcatac cggaagaact ggtgccggtg cagaaggtcg
     2881 gccgcatggt gctcaaccgc aaccccgaca atttttttgc ggaaaccgag caggtcgctt
     2941 tctgtactgc gcatgtggtg ccgggcattg atttttccaa cgaccccctg ctggccgggc
     3001 gcatccattc ctacgtggac acccagatct cgcggctggg cgggcccaac ttccatgaga
     3061 tcccgatcaa cgcgccgatt gcccaggtac ataacaacca gcgcgacggc atgcaccgcc
     3121 aggccatcca ccgcggccgg gtcgcctatg agcccaactc gctggccggc ggttgcccct
     3181 tccaggccgg tgcggcgcaa ggttttgtgt cggtggccgc ccgcctgcag gccaaagagg
     3241 accaggccaa ggtgcgcggc aagcccgaga agtttgccga ccactacacg caggccacgc
     3301 tgttttatga aagccagacg ccggtcgagc aggcgcacat cgcggccgcc ttccgcttcg
     3361 agttgagcaa ggtcaccgtg ccggccatcc gcgaacgcat ggtgggctcg ttgcgcaatg
     3421 cctcggaggc gctggcgcgc gaggtggccg acggcttggg catggagacc ttgccggcgg
     3481 cgctgccgcg ggcactcgcc aagccggcca agcccgaagt cacggtgtcg ccgacgctgt
     3541 ccctgctggc caggcccggt gacggctcca tccagggccg caaagtggct gttctggtcg
     3601 cgcccggcgt ggccggcgac tcgatcgcga aagtgcaggc cgcgctgttt gcgcagggcg
     3661 ccgtgccgcg cctggtggcg ccgcgcattg gaccggtggc cacgctggaa ggcgataccc
     3721 tggaggcgga cgcttccctg gaaaatgaac ccggcttcct gtttgacgcc ctggtgctgc
     3781 ccgacggcga gcaggcggcg gcggcgctgg ccagggatgg gcacaccatg gagttcatca
     3841 aggaccagta ccgccacggc aaaaccctgc tggtgctggg cgcctcacgc gccctgatcg
     3901 acaaggccgg gctgccggcc accttgccgg gcggcaaggc cgaccccggc ctgctcatgg
     3961 ccgacgccgc cacggcctcc agcacgatcg agactttcat ggctgcgctc gccgggccgc
     4021 gtcatgcagc aagggaaacc gacccgcccc tggtctgagc cccgggcaga tgtaaaaagc
     4081 ggccctcgag ccgctttttt ttcaaggttg gcaggcgctt gagcctgccc cggtgatcac
     4141 tcgaccttgg ccttatcgcg caggccttgc tggaaggctg cgagtttttg ctgttgcatc
     4201 tgctgggcga tctgcggctt gagttcttca aacggtggca gctgggcggt gcggatgtcg
     4261 tccacgcgga tgacgtggta accgaactgg gttttcaccg gcacgtccgt cagctggccc
     4321 ttgttcatct tggtcatggc ctcggcaaac tcgggcacat agctgctcgg gttggcccag
     4381 tccaggtcgc cgccgttggc gccggagccg gcgtctttgg aagacttctt ggcgatgtct
     4441 tcaaacttgc cgcctttttt caggctggcg atgatggtct tggcttcgtc ttccttttcc
     4501 accaggatgt ggcgcgcctt gtattccttg ccgccgttgg ctgcgacgaa cttgtcgtac
     4561 tcgcccttga ggtcggcatc ggtcaccggg ttttttttct ggaaatcggc gaacagctcg
     4621 cggatcagga tggcctggcg ggccagttcc atctgcgcct tgaagtcgtc gctggccgac
     4681 aggccgagtt tttcggcttc ctgcatgaac acttcgcggg caatgacttc ttccttgatc
     4741 tggccctgca tttcgggcgt caccggacgg ccggagcgcg ccagttgctg cgccagcgcg
     4801 tccacgcggg ccttgggcac ggccttgccg ttgacgatgg caatgttctg ggcaaatgca
     4861 cccgatgcgc ccacagccat caggccggtc agcgcgcttg ccagtaaaag atgctttttc
     4921 atggtttcct tggggactct ttggaggggt agaaagcggc cccgtttccg gggtgcccaa
     4981 atcagggcgg tttaatgatt tcaatggcca gtgcatgcag gccctggtct atggaatttt
     5041 gcagtgcatc atacacaagc cggtggcagg ccacgcggct tttcccggca aacgccgggc
     5101 tcgcaatccg cacgcgaaag tggctgccga agccttgggc attggcgccg gcgtggccgg
     5161 catgcgccgc gctttcgtcg atcacttcca gcaggctcgg gctcaggcgc tcgcgcaact
     5221 gctcctcaag ccaggccgca tgtacgggca gggcggtgga gggggtagat ggggtggagg
     5281 gcatcatggc tcggtcttga cgtgtttgtt caggaaaacc gcctgtgcca gcacaaaaac
     5341 cagcatcagg cccatgccgc cgaacagctt gaagttgacc caggtggccg tggggaagtt
     5401 gaacgccacc cacaggttga gcacacccat gaccgcgaaa aacgcgatcc agctgtagtt
     5461 gaccttgcgc cagatggcgt cgggcagcgt catctgcgcg cccatcagcg actggatgcc
     5521 gtttttgccg aacaccagct gcccgaccag cagcgcgccg cccatgaccc agtagagcac
     5581 cgtaggtttc cacttgatga agctttcgct gtggaaatag atcgtggcac cgccgagcag
     5641 ggtcaccaga gccaggctga cccagagcat ggtgtctatc ttgcggccac gcgccaccag
     5701 ccagaggatt tgtgccaggg tggccaggat caccaccacg gtggccagca gcaccggcgc
     5761 ctcggcggtg cccaccacgc cgccggagac catgaagcca agccagtcgg tcgccgtctg
     5821 ggccgaccag gcggtgttgg cctcggcgta cttgaacatg ccgaagaaaa gaagaatggg
     5881 caggaaatcg aacagaattt tcatgggcag attatcaggg gcaacgcccc gttcttttca
     5941 agcaggggcc gcggaaccgg cttcgccggg ccgcaggcgc agcgcccccg aggggctgga
     6001 gcctgcggct ccaaagctgc ttgggcagct ttggacaggc gacagaggcg aagcgtctcg
     6061 cgagccgcgg cctgcaaggc ctcgcgcagt acgcgcagcg acaagcgtgg gggctttgtc
     6121 actttttctg aaaatcaagc gaggcggaat tcatgcagta gcgcaggccg gtgggtgtgg
     6181 gaccgtccgg gaacacatgg cccaggtggg caccgcaatt ggcgcagact gtttcggtgc
     6241 gtgtcatgcc gtaggcgcgg tccacatgtt cttcgatcgc gccttctttg acttcctgtg
     6301 aaaagctggg ccagccgcag cccgcgtcaa acttggtcga ggcgtcgaac agttcggcgt
     6361 cgcagcagat gcagcggtag gtgccggg
//
LOCUS       DAIDFG010000178         6323 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_178_length_6375_cov_17.679589, whole genome shotgun sequence.
ACCESSION   DAIDFG010000178 DAIDFG010000000
VERSION     DAIDFG010000178.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 6323)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6323)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6323
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178_length_6375_cov_17.679589"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1083 a         2135 c         2023 g         1082 t
ORIGIN      
        1 tcccgctcag gcagcgccat agttgctgcg cttacacacc cagggccagc tgggtggtgt
       61 tcttgacctc ttccatcacc gcgtaggtgc gggtttcgcg cacgccgggc aactgccaga
      121 gtgcgttgcc ggcgaagtcg cggtaggccg ccatgtcggc catgcgggtc ttgagcaggt
      181 agtcaaagcc gccggccacc atgtggcatt ccatgatctg cgggtacacc tgcaccgccg
      241 ccttgaaggc ctcaaacaca tggggcgtgg tgcggtccag cagcacctcg acgaacacca
      301 tcatgccggc gcccagcttc aaaggattca gccgcgcctc gtagcccagg atgtagccgt
      361 cgcgcgtgag ccgctgcaca cgcgccagta cggccgtggg cgacagcgcc accgcttcgg
      421 cgagcttgag gttggagagg cgcccgtcct gctgcagcac gctcaagatc ttcaggtcga
      481 tgcggtcgat gtcgggcggg gtgtcggtca tggtagtgaa ttattcggag ttggtggctg
      541 aatttagcgt gaaattctca tgtaatccag agaacatggc gttattcacc agttcacgga
      601 gatttttcat gtcgcaacag ccccgccttc cttttcctta ccggcctgaa gccggcatcg
      661 ttgcccggca tcttcaggcg ctggctgggg cgctggactg ggccgccgcc gtgcccctgg
      721 cccagccttg ggtgcaggcc gtgcgcgaca acccgccgcc cttctgggcc atggaaagcc
      781 tgctcaagga gtacccgatt tccagcgccg agggcctggc cctgatgcgc ctggccgaag
      841 cgctgctgcg ggtgcccgat gccgagaccg cgattgccct gaccgccgac cagctgggcc
      901 gcgccgattt tgacggcgcc agcgacggca tgatggcgcg cctgtcggcc tcggccatcg
      961 ccctgtccaa gaagtttctg cctgaagatg gccagagcag catcatgagc aagctgggtg
     1021 cgcgcaccgt ggtggccgcc accttgcgcg cggtgcagtt gctggggcgg cagtttgtac
     1081 tgggccagaa catcggcgag gcgatggacg aagcccgcga cgcccaaaag aaagtggcca
     1141 gcctgcgcgt ttcgccgccg ggccgcccca aggcggaaca gccccctgag ggggcagcgc
     1201 agcccgcagg gcaagcgtgg gggctcagat tttcgtacga catgctgggc gagggcgcgc
     1261 gcaccgatgc ggatgcactg cactacctgg cgagttatga agatgctatt aaatccatag
     1321 ctgcccgcgc agacaggacg ggcacctgtg agcaaaacga cggtatctcc atcaagctca
     1381 gcgcgctgca cccacgttac gaagacgccc agcatgcacg cgtgctggcc gacctggtgc
     1441 cgcgtgtctg gggcttgtgc gagctggcgg cgcgcgccaa catcaacctg accatcgacg
     1501 ccgaagaggt ggaccggctc gaactctcgc tggacgtgtt tgaagcgctg gccgctttgg
     1561 tggcgcagca gcagccgcag tggcagggct tcgggctggc actgcaggcc taccagacgc
     1621 gtgcgctgga gctggtcggc cacgtcatca ccctggcccg caagtaccgg ctgcgtttca
     1681 tgtgccgcct ggtcaagggc gcctactggg actcggaaat caagcgtgcg caggagctgg
     1741 gcctgccgca ctacccggtg ttcacgcaca agcaccacac cgatgtgtct tacctcgcct
     1801 gcgcgcaggc cctgctggcc gcacccgacg cgatttaccc gcagtttgcg gggcacaacg
     1861 cgggcacgat cgccgccatt attcagatgg ctgcacgcaa tgccgtacca ttcgagttgc
     1921 aaagactgca cggcatgggc gagggggtgt tccgcgaagt gctgaaaaac ccgcagatca
     1981 gctgccgggt ctacgccccg gtcggcgccc accgcgacct gctggcctac ctcgtgcgcc
     2041 gactgctgga aaacggtgcc aactcctcgt ttgtgcacca gcttgccgat gagtcggtcg
     2101 gcatggatgt gctgctgact tcgcccctgc gcatggagcc cgagtcgtcc ctgccgctgc
     2161 cgcccgccct gtatggctcc ggccgtccca acagcgaggg actggacctc acggtcgaga
     2221 gcatgcgtgc acccctgctg gccgcgctgc agaccacgca ggtgcccgtg gtgccggagt
     2281 tcgatgcgaa attggccacg agcgcagtag cggtgggcgc ggacagctat caaaaatgga
     2341 gcaaagtgga ggtggccgaa cgcgccgcag tgttgcgccg cgcctccgat gcgctgcagc
     2401 aacagatgcc cgccttctgc gcgctgctgg tcaaggaagc tttcaagacc tggggcgacg
     2461 cggtgtccga ggtgcgcgag gcgattgact tcctgcgtta ctacgcgggc gaggccgaac
     2521 gcatcatggc gcccatcgcc ttgccggggc cgaccggcga aaccaatgag ctgcgtctga
     2581 gcgcacgcgg cgtctgggtc tgcatcagcc cctggaattt tccgctggcg attttcaccg
     2641 gccaggtcgc cgcggccctt agcaccggca acgcggtgct ggccaaaccg gccgagcaga
     2701 cgccaggcgt ggcctgggaa gcggtgaagc tgctgcacgc cgccggtgtg cccgaaggcg
     2761 cgttgcagct tttgcacggc cccggcgaaa ccgtcggtgc ggcgctggtg gcggcgccgg
     2821 gcgtggccgg cgtggtgttc accggctcca ccgaagtcgc caaaatcatc aaccgcgcgc
     2881 tcgccgccaa ggacggcgcc atcgtgccgc tgatcgccga gaccggcggc atcaacgcca
     2941 tgctggtcga cagcagcgcc ctgcccgagc aggtggtcga tgccgtggtg caaagcgcct
     3001 tccgctcggc cggccagcgc tgctcggcgc tgcgcctgct gtgtgtgcat gagggcatcg
     3061 ccgacaaggt ggtggccatg atccagggcg cgctgcagga gctggccgtg ggcgacccga
     3121 ccctgctttc caccgatgtc ggcccggtga tagaccgcga ggcgtttgac aacatccaga
     3181 accacctgca gcgcctggat tcaacatcaa aagtgcttcc agccctttct gcccatgcgc
     3241 agacagctcc tgattcaata gcaaatctgg ttctgcccag cgccttcgaa gtagccagcc
     3301 tggccgaggt gaaagacgaa atcttcgggc cggtgctgca gatcgtgcgc tggagcggcg
     3361 agccgcaaaa ggtgatagcc cagatcaacg ccctcggcta cggcctcacg ctgagcatcc
     3421 agacccgcat cgactcgcgc gcccaggcct tggccgcggc cgcgcatgtc ggcaacatct
     3481 acatcaaccg caacatgatc ggcgcggtgg tgggtagcca gccttttggc ggcgagggcc
     3541 ttagcggcac cggccccaag gccggcggcc cgcattacct gtaccgcttc tgcgccgaac
     3601 agaccgtcac cgtgaacacc accgcggcgg ggggcaatgc ggccttgttg tcgggggcgt
     3661 gagcgcttag ggaagcgctg aacaagtccc ctgcggcgcg cgatcagccg ggctcgggcg
     3721 gcctgctgcg ttgaatttct tgccaatagc cagctattgg ctgcgaaatc cgccttgcat
     3781 tccatcccga tccggctgcc gcgcatccgc aaggacatat tcagcgcttc ctaaagccta
     3841 aaatggtttg gtgatcccca aaaacatcct caacgctgat ctgcattgtc actccgtggt
     3901 gtcggacggc accatgaccc ccgaagcgct ggccgaacgg gccaaaacca acggtgtcga
     3961 actctgggcg ctgaccgacc acgacgaaat cggcggccag catcgggctg cagcggcagc
     4021 ccgggcgcag ggcatgcatt acctgaccgg cacggaaatt tcggtgactt ttgccggtga
     4081 aaccgtgcac atcgtcggcc tcgggttcga ccccgacgac gcggccatgc agcagggcct
     4141 gcgcaacacg cgcggtggcc gcgaacagcg cgccatggag atggcctact cgctggccaa
     4201 ggccggcatc cgcggcgcct acgaaggcgc gctgaagttt gtcggcaacc cggacctgat
     4261 ttcgcgcacc cacttcgcac gcttcctggt ggagaccggc gtctgcaagg aaacctccga
     4321 ggtgtttcgc aagtacctga ccgaaggcaa acccggctac gtgccgcacc gctgggccag
     4381 cctgcaggac gccgtggcct ggatcaccag cgccaatggc gttgcggtga tcgcccaccc
     4441 ggggcgctac aagttcaccc ccaacgaaga atacgcactg ttcaccgaat tcgtgtccca
     4501 tggcgggcag gcggtggaag tggtgaccgg cagccacagc acgcaggaat atgtgaaata
     4561 cgccgaaacc gccaaagagt tcggcctggc cgcctcgcgc ggcagcgact tccacagccc
     4621 cgacgaaagc cacaccgacc tgggcaccct gccctttttg cccggccacc tgacccccgt
     4681 ctgggagctt ctggaagacc gcatccggtg agccgcgcgc cgtcggccct ggcgggcatc
     4741 acgctggtca ttgcagccac cggttttttt gcaacgctgg acaccaccac caaatttgtc
     4801 agtgccagcg tgccgctgtt gatggccctg tggtttcgct actttttcca ggcgattgcc
     4861 accaccgccg tggtgctgcc gctgcgcggc ctgagcgtgc tgcgcaccgc ccacccgaaa
     4921 ttccagtgcc tgcgcggcct gctgctgctg ctcagcagcc tgttcgcctt cctgagcctc
     4981 aagtacatgc cggtggccga gttcaccgcc atcggcgcca tcgtgccgct gctcatcacg
     5041 ctgctggccg ctacggcgct cggcgaaaag gtctcggtcc tgcgctggtc gctggtgctg
     5101 ggcggttttg ccggcacgct gatcatcatt cgccccggcg gcgaagactt cacctggacc
     5161 ctgctgctgc cgctgggcat ggtggcaacc aacaccggct tccaggtact caccagccgg
     5221 ctggccaaaa ccgaagaccc gatcaccatg cacctgtaca ccggctgggt cggcacgctg
     5281 ctggcctcgc tggccctgcc ttttgtctgg atcaccctgc cctcgtggtc gttgtggggc
     5341 tggctgctgc tgatgggcgt gatggccacc gtcgggcatt tcatgctgat cctgggctac
     5401 atgcgggcgc cggccgccac cctcacgccc tacctgtatg cccagatcgc ttttgcgatg
     5461 gtcggcggct ggatcgcctt ttcgcatgtg ccggacggct ggtccatgat cggcatgctg
     5521 atgattgcca tctgcggggc actgggggcc tggctgaccg cgcgtgaaag ccgtgcgctg
     5581 aaagagttca gcgtgcggcc ggcagagtcc tgacatcctt gaaaaacaca ccatggccca
     5641 gtatttcgag attcaccccg acaacccgca agcgcgcctg ctcaaacagg cggtggcgct
     5701 gctgacgcgc ggcgcggtga tcgccgtgcc cacggattcg agctacgcgc tggtctgcca
     5761 ccttgacgac aagacggctg ccgacagcct gcgccgtatc cgcggcgtgg acgacaaaca
     5821 ccacctgacc ctgctgtgcc gcgacctgag cgagctggcc aactacgcac gcgtcgacaa
     5881 caagcagttc cgcctgatca aggccgccac gccagggccc tacaccttca tcctcgaagc
     5941 cagcaaggaa gtgccgcggc gcctgagcca cccctcgcgc aagaccatag gcctgcgcgt
     6001 gcccgggcac aaaaccctgc aggcgctgct ggagctgcat ggcgcgccgc tgctggccac
     6061 cacgctgatt ccgccgggcc aaaccgagcc gctgaacgac gcgggcgaca ttcgcgacca
     6121 catggaacac gacctggcgg cggtcattga cgctggcgcc tgtccccagg aagccaccac
     6181 cgtgatcgac ctgaccccca tgggccaggg tgatgacccc gtggtggtgc ggcaggggcg
     6241 cggcgacctc gcggcgttgg gcatttagag cccccacggt cgctcactgc gtgtagctcc
     6301 ctgccccccg agggggccgc tgc
//
LOCUS       DAIDFG010000179         6354 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_179_length_6354_cov_16.879187, whole genome shotgun sequence.
ACCESSION   DAIDFG010000179 DAIDFG010000000
VERSION     DAIDFG010000179.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179_length_6354_cov_16.879187"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1095 a         2010 c         2127 g         1122 t
ORIGIN      
        1 accgtggagc agaaggcggc gatgggcggc gctgcaagtc agggctctgc ggtggccggc
       61 ggcggcggtg ggctgcagga cgtcaccggc gtggtgcaga acatccaggt ggcgggcacc
      121 tcgaacggca tcaccaacac cataggcatc aacgtccaga acgcatcggg gcaacccgct
      181 ccgcagcccc aatcaaccgg cttgctctcg gcgtccacca ccacggccag tggcagcgtg
      241 gcgtccgtcg ggctgggcgg caacggcatg aatgtcgccg tcgccgttcc ggaccaggga
      301 cgggcaatgc agcagattcg ccaggtgggc atgggcggcg ggcaggtgtt gcagtcggtg
      361 cagctgggtg gggacgtcaa ccagattcac aacaggatca gcctcaatgt ccagatgaat
      421 tccgcgtccg gaccggcatc cgcggcgggc aaccagttgc tgggaatgaa gctgatgccg
      481 caaagcggcg ctttttgatc cgtccgggcg ggccaaccgt gccagtccgg aacgcgcccg
      541 gggatcgcac ggggcgctga agaggaaagc gccgcgttgc ggcggccagc tggttgggta
      601 atcgaaaaac tgatgaggga gatcttatgc gacgtttttt tccgctgcgg cccctggtgt
      661 tgaccttggc agccggcttc cccctggctt gcctggcgga caacgatggc gaagtggccg
      721 agctgcgccg gcagatacag gaattgaccc agcgcgtgga cacgcaccag tcctcgctgc
      781 gggtgttggc cgggcgtttg cagcagcttg aggcggctgg cccgggccaa gcgcgcttgg
      841 tgagaacggc agccgatggc tcccagcctg atgcgtcggc aagccgggac ccggcttccg
      901 cggagggtgg cggcgcagag acggtggtcc gggaagcgcc ggcttcgcgc agtgccgagg
      961 cggtttaccg cgaacagagc gccatgttcg accgcaaatt caccctggaa acgggcctga
     1021 gttattccca ctcaaaccgg cgggaccttt ttctcaacgg ctttctggcg ctggatgcga
     1081 tctttttggg acagatcaat cttgaccgca tcaaggccga caccctgacc tttgacctga
     1141 cggggcgtta tgcgccgacg gaccggctac agttcgacat caacgtgccc tatctgtacc
     1201 gttactccgc cttttcctcg gtcggcgcgg gtttcagcac ctcggcggtg gccgagagga
     1261 atgtctcgaa cggcgatatc ggcgacgtca atgccggcgt gttctaccgc ctggtccagg
     1321 aaagcgctga ccgccctgac accgtgctca actttcgcat caaggcgccg accggaacca
     1381 gcccctatgg catcaagttc gaggatgcgc cggattccgg gggcaacctg aaacttcccg
     1441 agaagctgcc gaccggcaat ggcgtgtggg cggcttcggc cggcgtgagc ttcatcaaaa
     1501 cggtggatcc ggccatcctg tttgccaacc tgggctacac gcaccacttc gaccgcagct
     1561 tcagcgatat cagttcggac ccgaacacga gaacgccggg cagtgtccgg ctgggggacc
     1621 agttttcgct gggcgcaggt tttgcctttg ccctcaacga acgcatgagc ctgagcacct
     1681 cgtattcgca ccgctttgcc agaaaagcac gcatccgcca gcagggacag gactggcaga
     1741 ccgtcaatgg cagcgactcc agcgccggcg tgctcaactt cggcgtgacc tatgccttgt
     1801 cggaccggcg gtccatggta ttcaacctcg gcatcggcgt caccgccgac gcgccggaca
     1861 tcaccatcgg ggtcaagttc cccttcagct tctgaccttg ccggcgtcgg tcagcaccac
     1921 agcgcgatgg caacgctcga agcgggccgt gcccggcttc aagcggttgg atctgccggg
     1981 tgaccgccga tcgggtgagg accaactcgg ctgcctcgtg cgtgaaaccg aaagggaggc
     2041 ctgccatgct tgacgattca aggccctttt tccctgcccc ttccccacca tgcgacgttc
     2101 ggtgtgtccg gtgccgccgg cgtacaaatc ctccgccacg aaaaaaccag ccggagggcg
     2161 gcgcgccccc gtagacggct accgtcctgt gccccggcct caggagcaga cggcttcgac
     2221 gttgttgccg tcggggtcga tcaggaaggc ggcgtaatag gtcgggctgt aatccggacg
     2281 cgggccgggt ccgccgttgt cgctggcgcc ggctttcagg ccggctttgt aaaaggcgtc
     2341 aacggctttg tggctggctg cacggaacgc gatgtgggtg ccgggaccct tggcgccctg
     2401 ggacgcataa agccacaagg ccggtgcgtc ggccgggccg atgccggcgt agctgtcgtc
     2461 gtgcgagctc agcacatggc ccagcggtgc gagagcggct tcgtagaaac ggatgctggc
     2521 tgcgaggtcc ctgaccttta tgccgaggtg gtcgtacatg gtgatttcct gcttgaagag
     2581 tggatcaagc ggtcatcatc gagcagtgcg gccatgcggt cttggagaat cttgcgctct
     2641 cctttcaggc cttttctcgc ggcctggcgg aaggcacgcg gcgagacgcc ggccgcgcgg
     2701 tggaaggtgc gcacgaagtt gctgagatcg gcaaacccga cgtccagcgc cacatcggtg
     2761 acaggccggt cgccgctggc cagcagccgc gcagcgtggc gcagccggga gcgcaccagg
     2821 tactggtgcg gcgagacgcc gagtactcgc gagaacagcc gcaaaaaatg aaacgggctc
     2881 aaccccgcct ggctggcggc gtgggccagg gcgatgtctt cctgcgaatg ggtggtgatc
     2941 cacatcgccg cgtccacggc gcgccggcgg tctctggcgg tggcgcgctg cggcgttgcc
     3001 ttgcggcctg tcaccacgtc tacaaaacgg gaggcgaacc acagaccggc ttcgtccagg
     3061 ccgacatcgc tctccttgca ggccgccgcc tgcgccaact cgcccagcac catgagttcg
     3121 ggcaaaggcg gcaggccgcc ggtgcgccag gtgacggcgt cgccgccgat caggtcgaca
     3181 aaacccggcg agagatggaa agccaggcat tcgtcgccgc agacatggtg gtcatgggtg
     3241 cacatgaact cgtcgcccgg gtggcccacc agcaccgagc cggcgaccag ctcataactc
     3301 tcgccgcgcg tgcgatagcc gaaactgccc ttgcgtacgt acgacaccga gtagccgctg
     3361 tgcacctcgg tgaatggcct ggcgtcggga ccggtgctgc agcggtagtc aacgacctcg
     3421 atggggccgg actgcagtgc ggtggcggtc agcatgaacg cctctccttc aggcctcgcg
     3481 caaggccttc ttgagctcct cgccaatctc cggcttgtgc gcaaacgggt tggtcgggtt
     3541 gcgcgcctgc aggatgtctt ccagccgcgt ctgcaccgag ccaatggcct tggggtggct
     3601 gtagatgtag aactggttgg cggcgatcgc gtcgaacacc ttttgtgcca cgtcggccgc
     3661 cgtcaccttg ccggagccga ccgccttgtc gctcatggcc tggccgatca attggctttt
     3721 ggtcggcctg gcggccggca gctcatcggg ccggttgcgc tggctctggc tgatgccggt
     3781 gggcacgaag aaggggcaca gcaccgaagc gctgatctgg tcggtgacca gcgcgaggtc
     3841 ctggtacagg gtttcgctca tgctgaccac cgcatgtttg ctgacgttgt atatgcccat
     3901 gttgggcgca ttgagcagcc cggccatgct cgcggtgttg acgatgtggc cctgccaggc
     3961 cgggtctttt ttggcggctt ccagcatcat gggcgtgaac acacgcacgc catgcgccac
     4021 ccccatgagg ttgacgccga tgacccattc ccagtccttc agcgtgttct cccagatcag
     4081 gccccctgcc ccgacaccgg cgttgttgaa gacaaaatgc ggcgcgccga aacgtgccag
     4141 cacggcggcg cccagcgctt cgacctcggc ggctttggac acgtcgaggc ggaaagccag
     4201 cacctgcgcg ccggcggcgg tcatttcagc cgcggccttg tcgagcgcat cctgctgcac
     4261 atcggcgagc acgaggttca tgccgagccg tgcgcctatg cgtgcgcact cgaggccgaa
     4321 gcccgagcct gcaccggtga gaacggcggt tttgcctttg aagtcactga tcatgtttgc
     4381 tcctgaaatg aaaggggtcg cagcatctcc acatgcggaa tgccgtcttc gatgtagggg
     4441 tctcctgctt tcgcaaaacc gtgctgcaaa taaaatttct caagccgtgc ctgcgcgcct
     4501 atacggatgg gctgcgcacc ccactgctca actacgcagg cgatggcctg gcgcatgagc
     4561 tcgtggccct ggccgcttcc gcgcaggatc tggcgggtga tcacgcgccc tatgctcgct
     4621 tcggcaaact tcacaccggg aggaaagcag cgcgcatagg ctaccagccc gccatcggcg
     4681 gtacccagaa ggtgcacggc cgcgtggtct gcaccgtcca tgtcctgata gatgccggcc
     4741 tgctccatca cgaaaacttc cgtgcgaagc tgcaggacgt cataaagctc ggtagtggtc
     4801 agcgccgcaa aggggacaga ccgccaggtg atgccgctca tgcctgggcg acagtcttca
     4861 tggcataacc gatgcgcggg aagtgcacat gcagcgtgcc ggcgcgctcg tcggtgcggc
     4921 tcaaggtgta gtgcgtacgt gtcgcggcga tcagctcacc ttcggtgggc tcggggccga
     4981 aactttcggc cgtgatgctg acgcggctgc ccagcggaat gccatgttcg tcctggaagg
     5041 tgctgtcagt caacaggccg tggccgaccg gcatggggtc ggcagctgcc gctgcagcga
     5101 tggcctggac cgcatcgaat ttttccatgc tgccgtggcc gatggcggcc atgcggtcca
     5161 tccactcgag tacggccggc gtgagctgca ggatgtcggc taccacgggc gtgcgcaccc
     5221 gggtgaacca cagcgggtgg taggcggaaa agtcggcgat gcagggcacc tggccgagca
     5281 ggaaaggttg ttcgtcgagc atgtccgaca agcgccgcaa gtaggacttg taagccgcgg
     5341 ctgcatcggc cggacgcagc cgcgtcatgt tgaaactcat ggccttgcgg tcttcgccga
     5401 aagccctggc ggcctcgggg ggcgcgccgg caaacatgtg cgcagcgcct ttggggccca
     5461 ggttgtgcgc catggcggcc cagaacagtg tgctgtcggc ccactgcgcg atgatgcgcg
     5521 ccagaccctt gcccggctcg gggtagagcg aaggcgcggg ctgcaggtgc tcgagcacat
     5581 cggcgatcag cgcgctgtcg caatagatgt cggcaccgac ctgcaaaaac ggtgtcttgc
     5641 ggtagccacc ggttagcgcc agcacgtcgg gcttgggcat gatgctgggg atgatcaccg
     5701 acttccaggc cagttgcttg tagccgagga tgaggcggac cttctcggaa aacggcgagg
     5761 tcgggtagtg gtgaagaatc aggtcggcca tgggagctcc agttgaccgt catcccgggc
     5821 ttgacccggg atccatgacg gtgtgccagg gatggattgc ggatcaagtc cgcaatgaca
     5881 ggaacacaaa ttcatcgcgg catggcgcgg ttgatcaggg cgtcgaaatc cacatcggcg
     5941 cccagagtgc cgaaagcctg gccccagtcg ccgcccagcc gtgaatcgca gaaagcctcg
     6001 aacacggcgg cgggcgcgct ctggtacagc agcgcggcct gcacggccag cgccacgtcc
     6061 tgcgccagcc gccgggcctc catttcgttg gccatctcct cgacacgggt cggcaaggcc
     6121 gcggccagcc ggtccagcgc cgggtgggcg ccttgggcgg gtgccagttc ctgcgccagt
     6181 gcggcggcgg catcggcctt gcgcagtgcg cgcaacaggt ccagcgccat gatgttgccg
     6241 gcaccttccc agatcgagtt gagcggcatc tcgcggtaga tgcgggccat gatgccttcg
     6301 ccgccctcct ccacataacc gttgccgccc aggcattcca tggcttcctg cgca
//
LOCUS       DAIDFG010000180         6334 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_180_length_6334_cov_19.565377, whole genome shotgun sequence.
ACCESSION   DAIDFG010000180 DAIDFG010000000
VERSION     DAIDFG010000180.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6334)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180_length_6334_cov_19.565377"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1072 a         2020 c         1986 g         1256 t
ORIGIN      
        1 gagcgcgaag tggagctggc cgaagagatc gtcaagctcg tgccgtccat cgagatgctg
       61 cgcctggtga gctctggcac ggaagcaacc atgagcgtga tccggctggc gcgtggcgcc
      121 accgggcgca gcaagatcat caagttcgag ggctgttacc acggtcactc cgacgcgctg
      181 ctggtgaaag ccggctcggg cctggccact tttggcagcc cgaccagcgc cggcgtgccg
      241 cctgaagttg tgcaacacac gctggtcctt gagtacaacc acatcgccca gctcgaggaa
      301 gctttttcag tgcacggcaa ggacctggcc tgcctgatga tcgagccgat tgccggcaac
      361 atgaactttg tgcgcgccag tgtgccgttc atgaagcgtt gccgcgaact ctgcacgcaa
      421 tacggcgcgc tgctggtgtt cgacgaagtc atgaccggct ttcgcgtcgc cttgggtggc
      481 gcacaaagcc tgtatgccca gagcattccc ggcttcaaac ccgacatgac cgcgctgggc
      541 aaagtcatag gcggcggcat gccgatggcc gccttcggtg gcacacgcgc cgtgatggaa
      601 ctgctggcac cgctgggctc ggtctaccag gccggcacgc tgtcgggcaa cccggtggcc
      661 acggcctgtg gcctggcgac cctgcgcgag ctgcaaaaac ccggctttta tgaagcgctc
      721 ggcgagcgca cccgcagcct ggtcagcggc ctcacccagg ccgcgaccca atccggcgtt
      781 gctttttgcg gcgacagcga aggcggcatg ttcggttttt tcctgctgga caaactgccg
      841 cagaactacg ccacggtgat gacgaccgac agccccgcct tcaaccgctt cttccatgcg
      901 atgctggatc agggcgtgta ttacgcgccg gcgctgtatg aagccggctt tgtcagttcg
      961 gcgcattcgg cggccgacat cgccgagacc gtcgaggctg cggcccgatt ttttgctgca
     1021 cgctgaacgc cgcgcaagcc ggccaaagaa caggcttcgg cccgtccaac cccgggcagg
     1081 cgccccgatt gctatcaatt agatagctgt ccgcgcccgt ggagtaaggg atgcaggcac
     1141 ttttattaat aaaaaaccgg tatcaatgcc ggaagtgccg cacgccgctg agtaccatca
     1201 ccacgccgcg ttcgtcggcc gcatcaatca cttcctgatc acgcatgctg ccgcccggct
     1261 ggatcacgca gttcgcgcct gcatcgacca ccacgtcgag accgtcgcgg aaaggaaaga
     1321 aagcgtcgct cgcaacgacc gtgcccttga gcgacaggcc ggcatgcccg gccttgatgc
     1381 tggcaatacg tgccgagtcc aggcggctca tctgtccggc gccgacgccc atggtcatgc
     1441 catcggcgca aaacacgatg gcgttggact tgacgtattt ggcgaccttc caggcaaaca
     1501 gcaggtcctg cagctgggcc ggcgtcggct gcagcttgct gaccaccttg atgtcggcca
     1561 gcgccagttc gtggttgtcg gcggtctgca tcagcaggcc ggagccgacg cgcttgatgt
     1621 ccatcaggtt gcggccctgt tgccacgccg tgtcgccacc cggcggcagg tcaatctgca
     1681 aaatccgcac attgaccttg gtcttgaaca cttcgagtgc ttcgggcgtg aaggccggag
     1741 ccatcagcac ctcgacaaac tgtttggaga tctgctgcgc cgccgctccg tcgagcgtgc
     1801 agttcagcgc aatgatgcca ccgaaggccg aggtcgggtc ggtcttgaag gccttgctgt
     1861 aggcttccag cgcgtcggcg cctatggcca caccgcaggg gttggcgtgt ttgacgatga
     1921 cacaggccgg cccgttaaaa ctcttgacgc actcccaggc ggcatcggca tcggcgatgt
     1981 tgttgtagga gagccccttg ccctgcaact gcctggccgt gaccaacgag cccggcgccg
     2041 ggtacaggtc gcggtacagc gcggcctgct ggtgcgagtt ttcgccgtac cgcagttcct
     2101 gcaccttgat gaagcggcca ttggcctggc cgggaaacag gctgtgcgtg ccgtcgggct
     2161 ggaggcccga gaggtagtcg ctgatggcgc cgtcgtactg gctgatgcgg ttgaacgcag
     2221 ccaccgacag ggcgaatttg gtggcatcgg ccagcttgcc ggtcgacttg agctcctcaa
     2281 gcacgccggc gtactgcgat gcgtcagtca gcacaccgac atctttccag ttcttggcgg
     2341 ccgaacgtac catggccggc ccgccgatgt cgatgttttc aatcgcgtct tccagcgtgc
     2401 agccggcctt ggcgacggtg gcctcgaacg ggtagaggtt gacgaccagc aggtcgatgg
     2461 tgccgatacc atgggttttc agcgcagcca tgtgttcggg cacatcgcgg cgcgccaaca
     2521 ggccgccgtg caccatcggg tgcagggtct tgacgcggcc atcgagcatc tcggggaaac
     2581 ccgtcagctc tgcgacctcg gtcacaggca gcttttcgtc ggccagcagt ttggcggtgc
     2641 cgccggtgga gatcagcttg atgccgaggg cgtgcagcgc gcgtgcgaag tcaacaatgc
     2701 cggttttgtc ggagacggaa atcagggcgt tcatgcgttt ctttggtttc ttgggatggg
     2761 gtgcgacggc cgggtcactt ggggtcactt gatcagtttg tgttcaacga gttttttgcg
     2821 cagggtgttg cggttcaggc ccagccattc ggcggcgcgt gactggttgt ggtcggcatg
     2881 ctgcatcacc acctccagca gtggcttttc gaccacacgc accatcatgt cgtacatgcc
     2941 gtccggttcc gtgccgcgca agtccttgaa ataaccttcc aggctggtgc gtacacattc
     3001 ttctatgtgt ttcttgctca tgcgggctcc ccctccttct ttttctgttc tgcattgtcg
     3061 ccgactgccg gctgcgccat gccggcaccc accacaggca tgcggtccat gcgcaggcca
     3121 agcccgtcaa aaaagtccgc cactgcctgc cactgcgcct ggctgtcttc cagcaggttc
     3181 atgcggctgc ggaaagcctc gccgcccggc agcgtcctga cataccagcc gatgtgcttg
     3241 cgcgccgacc tcacgccgct gaagtcgcca tacagctcat agtggtccag caggtgatcg
     3301 agcagcaggc gtttgacctc ggccaccagc ggcggcgcaa gatgcgtgcc ggtcgccagg
     3361 aaatgcgcga tttcgcgaaa aatccagggc cggccctggg cggcgcggcc gaccatcagc
     3421 gcgtcggcgc cggtacaggc cagcacatcg cgggcttttt ccggtgtgtc gatgtcgccg
     3481 ttggcgacca ccggaatggc caccgcggcc ttgacggcgg caatcgtgtc gtactcggcc
     3541 tggcccttgt agccctgctc acgcgtgcgg ccgtgcacgg tgagcatctg cacacccgca
     3601 tcctcaaaga cacgggccag cgcgacggca tttttgtgcg attcggccca gccggtgcgc
     3661 attttcaggg tcacgggaac accgcgcggc gcgcaggcag ccaccacggc ctgaacgatg
     3721 gccagtgcca gcggctcgtc ctgcatcagg gcggagccgg cccacttgtt gcagaccttc
     3781 ttggccgggc agcccatgtt gatgtcgatg atctgcgcgc cgcggtcaat gttgtaggcg
     3841 gcggcctcgg ccatcatctg cgcgtcggtg ccggcaatct gcaccgcaat cggcgccacc
     3901 tcgccatcgt ggttggcacg gcgcgaggtc ttcagggtgt cccacaggtc gcgccgcgaa
     3961 gtgaccattt cactgaccgc gtaacccgct ccccccgcct tgcacagacg ccggaatggc
     4021 cggtcggtga cacccgccat cggcgcggca aacaggttgt tcgccaaaac gtaggggccg
     4081 atgttcatgg gaatggaagg cggtgtggat gctcaaaaac gaggcacgat tgtaactgcc
     4141 caaaatttca gcaattgaaa tctgaaaacg gggtgcgcaa gcccgctttc gggccaggac
     4201 gcgggcgcac tgcctatact cgcaccagat ggaactctgg atacaacacc tgattgaagc
     4261 cctggctctg cccgagtacg ggttgagcac ggtgtttgtg gtctcgttca tctccgctac
     4321 cctgttgccg ctgggttccg agccggtggt cttcggcctc gtcaagctca gcccgcacct
     4381 gttctggccg gccgtgctgg tggccacggc gggcaacacg ctgggcggcg cgctcgactg
     4441 gtggttcggc tatggcgccc acaaggtggt ggacaagtat tcgcactcct cacaccacag
     4501 ccgggccatt cgctggttgg aaaaactggg acccaaagcc tgcctgctgg cctggctgcc
     4561 cggggtcggt gacccgctgt gcgcagtagc gggctggctc aaatttccgt tctggccctg
     4621 tgtggcctac atggccattg gaaaattcgc ccggtacgtg acgatgacag gcgcactggt
     4681 ctgggccttt ccgggcaact tcaagtttct gggcggctag aaccgctcgt gcggctgcag
     4741 atagcgccac tgcccgaccg gcaggctgct catggacacc cgcccgacac ggatgcgctt
     4801 catcgcctgc accgtgagcc cgacgctttc acacatgaag gcaatttgcc cggggcgttc
     4861 gcccttgagc gcaaaacgca gacgggtctc gttttgccag ctcactttcg ccggcggcaa
     4921 caacaccccg ttgaaggtga aaccatggtc gatgcggttg agccgctgca ggccgcccgg
     4981 cgtcatggtg ccggtcacgt ccacgatgac ctcgtgctcc atgaccgacg cgtcctcgct
     5041 gagcttgcgc gccacgcgcc agtcctgcgt gaagaccacc aagccgccgg ccggggtggc
     5101 gagcggtgtc gccagcgtca atgcgctgac atgttttttc aggggccgga tgcccgaacg
     5161 gtcgtctttc gcctgattgg ccaccaccaa cagctgcgca gccgggttgg cagggcgctt
     5221 gctccctgca tccaggccgg cctcgtagcc gggcggcttg ttgagcaaca gcgtcaccgg
     5281 cgtcagcgcc agcaggctgg cgtccgggtc aagttcgact ttctggtcca gcacgcggtg
     5341 ctgcggctct tccacaacca caccatcaac gcgcacccag ccgccttcga tgtactgctc
     5401 ggcttctcgg cgtgagcagt tgagcatctg ggccaggtgt ttggcgaggc ggacgggttc
     5461 tgtcatgcga tctgctcggt cctttcaagc gccaccgtcg aaccggctcc gccgggcgac
     5521 aggaggcgtc ccctgcaagg ggaggatggg ctacacgcag tgagcccagg caggggtgta
     5581 ctcatctagc tgctgaacag gaaattcatc acgtcgccat ccttgacgac gtattccttg
     5641 ccttcgctgc gcatcttgcc cgcatccttg gcgccctgct caccctggta ggtgatgaag
     5701 tcgtcaaagg caatcgtctg ggcgcggata tagccttttt caaaatcggt gtgaatcacg
     5761 ccggccgcct gcgggccagt gtcgccgaca tggatggtcc aggcgcgcac ctctttcaca
     5821 ccggctgtga agtaggtctg caggcccagc agcttgtagg ccgcgcggat caggcggttc
     5881 agcccgggct cttcctggcc gatctcggcg aggaacattt ttttatcctc ttcctccatg
     5941 tcggccattt cggcttccat cttggcgcag atggcaacaa caggcgcatt ctggctggcc
     6001 gcgtaggcgc gcagcttgtc caggaagggg ttgttttcaa agccgtcttc agccacattg
     6061 ccgacgaaca tggccggctt ggcggtgatc aggaaaaact gcttgagcag tggcagctct
     6121 tccttgctga actccagcgt gcgcacgggt ttggcttcgt tgagggcggc ctggcatttc
     6181 tccagaatcg ccaccagctt gatcgactcc ttgtcgcccg agcgcgcggt tttcgttacg
     6241 cggtgcaggg ctttttcaac cgctgccaga tcggccaggc acagctcggt ctggatcacc
     6301 tcgatgtcgg aaatcggatc caccttgccg gcga
//
LOCUS       DAIDFG010000181         6207 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_181_length_6207_cov_17.697172, whole genome shotgun sequence.
ACCESSION   DAIDFG010000181 DAIDFG010000000
VERSION     DAIDFG010000181.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6207)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181_length_6207_cov_17.697172"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1119 a         2014 c         2031 g         1043 t
ORIGIN      
        1 ctacatccgg cacaaagtcg gcaaaaaagt aaggccgatc cggtgatttt gcggaacagc
       61 atcctatttt tgcggaagtt ttgctataat gttgatagcg aactatcgtt attggctatg
      121 gtagggcgtc acagacttga actgtggacc aaaggattat gagtcctctg ctctaaccaa
      181 ctgagctaac gccccacgtc gccagtgccg attgtaccgg ccctacttgc tgtggctcag
      241 tgcgctgctg accatcttgg ccgtgatgtc gacgatctgg atcatttttt catagggcat
      301 gcgcatgggc ccgatcacgc ccaaggtgcc cacgaccttg ccgtccacct cgtagggtgc
      361 ggtcacgatg gacagctcct ggtagggcac gacctggctt tcgccgccga tatagatgcg
      421 cacgccctcg gcccggctgg acacatcgag caggcgcatg agctgggctt tctgctcgaa
      481 caggtcgaac aacttacgca ggttgcccat gtcgctggaa aaatcgctga ccgccagcag
      541 gttgcgttcg cccgagatca ccacctcgtc gcgcgactcg atcgcttcgc tgctgacctg
      601 cacggccgcc tgcatcaggc tggcgatctc gccgcgcagg gcttccacct cgctccccag
      661 ccgctcgcgc acttcctcga tcgccatgcc ggcgtaatgc gaattgagga agttggccgc
      721 ctcgatgagc tggctctggg tgtagtcggt ctcggtgaag atcacgcggt tctgcacatc
      781 gccgtcgggc gaaacgatga tgaccaggat gcgtttttcg gacaggcgca gaaattcgat
      841 gtggcggaac acggaggtgc ggcgcggggc catcacgaca ccgacaaact gcgacaggtc
      901 cgacaggatg ttggctgcgt tgctgaaaac cttttgcggc tggtccggtg ccaggcgggg
      961 tgtgcccagc gcgctgagcg acgaatactg gtcgcgctgc atggtcagca tggtgtcgac
     1021 aaacaggcgg taaccgcggg cggtcgggat gcggccggcc gaggtgtgcg ggctgacgat
     1081 caggcccaga tcctccagat cgctcatgac attgcgtatg gtcgccggcg acaggtccag
     1141 tccggaggcc ttggacaagg tgcgcgaacc gaccggttga ccgtcggcga tgtagcgctc
     1201 cacaagcgct tttaacaaca gtcgggcacg ttcatcaagc atgaagccat tctacgaagc
     1261 ttggcaagga tgttgtgatg taatttggcg atgaagtcgc aatttcgtca cgtcgccctg
     1321 atcgggaagt accacgcaca gggttcactc tcggcgctgg aggaaattgc cggcttcctg
     1381 cacacgcagg gttgcgaggt cacgctggaa aaagacacgg ccagcaacac cggcctgatg
     1441 cactacccgg tgctcgatgt ggccggcgtc ggtgcgcatt gcgacctggc cctggtggtc
     1501 ggcggcgacg gcaccatgct gggcatagga cggcagctgg cccagttcgg tgtgccgctg
     1561 gtcggcatca accagggccg cctgggcttc atcaccgaca ttgccttcga ggatttccag
     1621 acggcgctcg agcccatgct gcgcggcgaa tacgaggaag accggcgctg gatgatgcag
     1681 gccgaagtca tgcgtgacgg ccgctgcgtc ttcagtgcca ccgccatgaa cgacgtggtc
     1741 gtcaaccgcg gcgccacggc cggcatggtc gagctcaggg tcgaagtcga tggccgtttt
     1801 gtggccaacc agcgcgccga cggcctgatc attgccacgc ccaccggctc caccgcctat
     1861 gcgctgtcgg cgggcggtcc gctgatgcat ccttcgattt ccggctgggt catggtgccg
     1921 attgcgccgc acacgctgtc gaaccggccc atcgtgttgc ccgacaccgg cgaggtcacc
     1981 gtggaaattg tcgcgggccg cgatgccagc gccaattttg acatgcagtc gctggccacc
     2041 ctgctgcacg gcgaccgcat cacggtgcgg cgctcggaat accagatgcg tttcctgcat
     2101 ccccggggct ggagctactt cgacaccctg cggaaaaaac ttcactggaa tgaaggtgcg
     2161 gtatgagtct taaaaacatc gccctgcgcg actttgtcat cgtgcgcgaa ctcgcgctgg
     2221 acctggacag cggcttcacc gtcctcaccg gcgagaccgg tgccggaaaa tccatcctga
     2281 tcgacgcact gcaactggcc ctgggcgcac gcgccgacgc cggtgtggtg cgtgagggcg
     2341 cgcagcgctg cgagatcagt gccaccttcg acgcgccggc cactctggcc ggctggctgg
     2401 accaggccgg ctttgccgca gaagacgact tgctgctgcg ccgcacgatc gacacccagg
     2461 gcaaaagccg ggcctggatc aacggcagcc cggccacggc cacccagctg cgcgagatcg
     2521 ccgacctgct ggtcgacatc cacggccagc atgcctggca aagcctgacc cggcccgagg
     2581 cggtgcgcgg cctgctcgat gcctacgctg gcgtaccggc ccaggccctg gcgcagccgt
     2641 ggcaggcctg gcgcaacgcg cacaaggccc tgcttgacgc gcgccaggcg caggacagcc
     2701 tgcagcgcga acgtgaacgc ctggcctggc agatcggcga actcgacaag ctggcaccgg
     2761 gcgagcacga gtgggaagaa ctcaacacgc agcacagccg gctgtccaat gtgcaggcct
     2821 tgcgcgatgc agtgcagact gcggtcgatg cgctgcagga gtccgacgac aacgccagcg
     2881 ccctgctggg ccgtgccctc accgcgctac agaatcagga gcatattgag cccgaattca
     2941 aagggctgat cgatgttttg agcagtgccc tggcgcaggt tgaagatgcc gtgcattcgc
     3001 tgaacggcta cgcgcggcat gccgagctgg agccgcagcg gctcgccgaa ctcgatgagc
     3061 gcctctcgct gtgggtcagc ctggcacgcc gctacaagcg cacgccggcc gagctgccgg
     3121 aactgctgtc cgcctggcgc agcgagctgc aaaaactcga tgcggcggcc gacctcgtcg
     3181 gcctggagaa aaccgagcaa ggggcccagg ccgtctacat ggacgcagcg cgccaggtgt
     3241 cgaaagcccg cagcaaggcc gcgcccctgc tggccaaggc catcacacag gcgatgcagg
     3301 gcctgggcat gcagggcggc aagttcgaag tggccttggg caagctggag cagcccgcgc
     3361 agcatggcct cgagcaggca gaatttctgg tggccggcca cagcggcagc acgccgcggc
     3421 cggtcggcaa ggttgcctcg ggcggcgagt tgtcgcgcat tgcgctggcc atcgccgtga
     3481 ccaccagcca gctcgggcag gcacagaccc tgatttttga cgaggtcgat tcgggcgtcg
     3541 gcggcgccgt tgccgaaacc gtgggccgcc tgatgaagca gctcggcaag gaccgccagg
     3601 tcatggccgt cacccacctg ccgcaggtcg cagcctgtgc cgaccatcac ctgatggtgg
     3661 ccaaacgccc ggacaagggc cagaccgtga gcaccgtcga gctggtccac agcgagcagc
     3721 gggtggccga ggtggcccgc atgctgggcg gcgaaaagct ctccggcacc acactggccc
     3781 acgccaagga gatgctgggc caatgaacac cgccaccgac ctcaccccgg acagggctcc
     3841 gagcatggcc ccggatatgg acatggtgct gatcaccggc atgtccggtt ccggcaaatc
     3901 catcgcgctg catgccctgg aagacgcggg ctactactgc gtggacaacc tgccgcctga
     3961 gttgctgctg ccttttgtcg cgctggaaaa gcagcacggc gccagcaagg tggcgatcgc
     4021 aatggacgtg cgcagcgcca cctcgctgcc gctggtgccg ctgcaactgg cccagatgag
     4081 ggaagaaggc gtgtccgtgc aatcggtgtt tctcgacgcc agcaccgaca cgctggtgcg
     4141 gcgcttttcg gaaacccggc gcatgcaccc gctgtcgcgg cgggacgacc tggaccagca
     4201 gcgggccctg gtcgatgcga ttggcgagga gcgcgaactg cttggcgagc tgcgcgagca
     4261 tgcccatgtg atcgacacca gcatgatccg gccgtcccag ctgcagggtt atgtgaaggc
     4321 gctgatttcc tcaccggcca cccagctgac cctggtgttt gaatcgtttg ccttcaagcg
     4381 cggcgtaccc gtcgacgccg actatgtgtt tgacgtgcgc atgttgccca atccgcacta
     4441 cgaaccggag ttgcgccgcc tgaccggcaa ggacgaagcg gtggtgagct acctgcaagg
     4501 ctaccccgag gtgggcgaca tgttccgcca catcgagcag ttcatcggcc actggctgca
     4561 ggcactggcc cgcgaccacc gcagctatgt gacggtggcc atcggctgca ccggcggcca
     4621 gcaccgctcg gtctacctgg tcgagcagct ggcgcaggcc tttggcccgc actgggtcac
     4681 cctcaagcgc caccgggaac tcgacgccgc gccgtgagcc tggccgccgc gactcagtta
     4741 ctctcttttt gatagctgcc tgcgcccgtt cctcaacggc tggaagcctt tttcatcaat
     4801 aaccgcggag gatcaggcct cgcgctgcgc gagcagcttc ctgatcggtg caggcagtcc
     4861 cagggcctcc caggcgccgc gcgcaaacca ggcccccgtg cccggcaggc ggatgcttct
     4921 ggcctcacgg gccgacagcg ccagcaccat gggcgacagg cgcaggtcct tgtgggtcag
     4981 cacatgcaca aagggctgga tcgccgcgag gcgggactgc agggacgcgg gcaaggccgc
     5041 cagcaaatcc tcttcgctgt cgaacaccgg aaaacaataa aggccgcccc agatgccggg
     5101 cgcagcgcgg cgttccagcc acacgctacc gtcggctttt tcagcccaca gcagcgagag
     5161 cgactgcgcg ctgcgcttga gcttgcgggt cttgaccggg aaactttccg gcgtgccgcc
     5221 ggccaggccc cggcacatgc cctgcaccgg gcacagcagg caggagggct ggcgtgttgt
     5281 gcaaatcgtg gcgcccagat ccatcaggcc ctgggtgtaa cgcggcatgg tctcaggcag
     5341 ctggcggcgc ggcagcagct cggtggcgat ggcccacagt tcccgctcct gcgcgctgga
     5401 ggccagatcg ccggaaaacg cggtaacccg tgtcaacacc cgcttgacgt tgccgtcgag
     5461 gatggccacc cgctccgaaa aacagaaaga ggcgatggcc gcagcggtcg aaggcccgat
     5521 gccgggcagg gtttgcaaca gctccgccgt ctgcggaaac tctccgccat gcaaacgcac
     5581 gacgtcctgt gcacagcggt gcaggtttct ggcccggctg taataaccca gcccgctcca
     5641 caaacccagc acatcgtcct gcgcggcggc ggccagatcg gccaccgccg gaaaccgcgc
     5701 caggaagcgg gtgtagtagt cgagcaccgt ggccacctgc gtttgctgga gcatgatctc
     5761 tgacagccag acgcggtaag ggtcacgcgt gttctgccag ggcaggctgt ggcgtccgtg
     5821 gcttttctgc cagcgcacga cctgcgtgga aaaacggctg cccagtgccg ccgcctgcga
     5881 accggtttcg gtcgccccgg aaagcagcgg gggcacaccg gcgccgggcg tggaggtcag
     5941 ggccatgccc gcgctcaggc ggcctggatc attgctgcgg ccggagccgc cagagccggg
     6001 tcatgcggac tggccggtgc atgcacgagc tggtccgtca gctcgtcgag cttgccgtcg
     6061 agttcatcga gcgcacgctc gtgagcggca atttcagcaa tgcgttcgtc caggccggtg
     6121 gcggcctgct ggatgcgttc gatcgcctcg atgcggcggc tgaaattctt gcggcgttcc
     6181 ttgagttggg catccagctg agaagcc
//
LOCUS       DAIDFG010000182         6206 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_182_length_6206_cov_16.236059, whole genome shotgun sequence.
ACCESSION   DAIDFG010000182 DAIDFG010000000
VERSION     DAIDFG010000182.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6206)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182_length_6206_cov_16.236059"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1054 a         1893 c         2122 g         1137 t
ORIGIN      
        1 gaggccatga ccacgctggc gcagtcggta agcggaatga agcctatctt gacttccttc
       61 ttctcgggcg cgtcggatcc ggccgcccag gcgccggggt tgccgatgcc gaaggtggcg
      121 gcggcgccca gtgcaccggc tttgcggatg aagtcgcggc gtgtcggctt gcctggagcg
      181 ggggaagttt gcgtcatgtg gaactccttg agtgacaaaa cagaagactg aaaaagaagc
      241 gcgaaaaatc aaaagaggaa aaacaaaacg gcgcccattt ctggcctgcg caagcaggct
      301 ggaaatggac gccgttgtcc ggtgaagccg tggaagactt ttgaaacgga ccgtcgttgg
      361 ccccttcatg ccatcaacta agcagtaacc gtgccagatg atttcgatgt ggggggtggg
      421 ctggtgcttg cctctgaaaa acggcttggc gcggcagcgc cgggagcgca ggacacgttc
      481 agccggggtg cgccatcctg gtgcgaatgc cctcaggcgg cacaggcctt gtgctgaatc
      541 agggcgttcc ggtggccagg tagtccgcca tggccagcac ggcttcggcc acgtcggtca
      601 ggcggcggtt ctggttcatg gcggtttgcc tgagcatctt gtaggcctct tcttcactga
      661 gcttgcggtg cgccatcagc aggcccttgg cgcgttcaat gagctttcgc tcgttgaggg
      721 aggcgcgcgc cgtctccagc tcgtcgctca tggcctgcag ccggctcgcc tggtcctgga
      781 ccagctcgag gatggagcgg tccaggtgcg ggccgtaagc agtggccgcg ggaccggtct
      841 ggcccgttcc tgccggcagg ccgccgccct gtgccgtgag cgcggcgagg atcatctggt
      901 ggtcgcgcag ttcggcgcgg gcctgcacga tcttctgttc gcacagcagc aggagttcgg
      961 ccgccaactg gtcctcgacg gttttcatgg cgtcgatgcg gtgggtgcag cagtcgaacc
     1021 aggtctggct caggttgggg tcaagggcgc cggccgccac cgagcaggcg atgcggcgca
     1081 ggcgttcgag gtctgccagc gtggcggtgc tctggctggt ctgccagagt tcgcgcagcg
     1141 ccggtgtcga aaaatcgttg aacacctgca cgcagcgttc ctgcgactgg atcaggtgca
     1201 gccactgctg ctggcgctgg ctgtcggtct ggcccgaagc gaaggtggcc gcgccaaacg
     1261 cgcgttcctg accggcaaac tccttgccct gcatgaaatt gaacatggcc accagcaggc
     1321 gcgagatctc ggggttggtg gcgctatcgg ccgcttcaaa gaccacggcc agcaggccgg
     1381 cgatcagctt gacaaaggcc gcggtggcct cggccgcggc cacgttctgc gtgctgatgc
     1441 gggcccgcag ggcgggcagg gcgtcgagct cgtgcaggac aaaggcaatg cggctgaaca
     1501 gccgggcgcc gttgttcagg tggccggcat cggtgtccag gtggtcgaag ctggcgcgca
     1561 actcgtcctc gacctgctgg caatcgagca cctggcgggg caactgtgcg gcgaaccgga
     1621 cgccctgcga gcccagaaaa acattggaaa tgccgcgttc cttctgcagc gcgtggacca
     1681 gccgcccgat cacgttgacc agcgcgctgg tgcgggtgag ttgctcgagg tcgttgattt
     1741 cgcaacgcct ggcggcaatc agaaagctca atccggattt catcgtttca gtcaaaggaa
     1801 ggaaggttcg ccattgtcca gcaacattca tgcctcgggc cgcttctggt ccggatccgg
     1861 ggcgaacggc acaccgcgct ttacactggg tgccatgctt gtactgggaa ttgaatcgag
     1921 ttgtgacgaa accggcgtgg cgcttgtcga cgcctcaggc cagggcgtgc cggccttgct
     1981 ggcgcacgcg ctgcacagcc agatcgacat gcaccaggcc tatggcggcg tggtgcccga
     2041 actggccagc cgcgaccaca tccggcgtgt gttgccgctg atcagcgagg tgctgggcca
     2101 ggcgcagcgt tcgctggccg acgtcgacgt ggtggcctac acgcgcggcc cgggcctggc
     2161 gggcgcgctg ctggtgggtg cgggcgtggc ctgctcgctg gcggcggcct tgggcaagcc
     2221 ggtgatgggc gtgcatcacc tcgaggggca tttgctctcg ccttttttga gcgccgaccc
     2281 cccgcagttt tctttcgtgg cgctgctggt gtcgggtggc cacacccagc tgatgcgggt
     2341 ggaccgggtg ggcagctatg agctgctggg tgaaaccatt gacgatgccg ccggtgaggc
     2401 atttgacaaa tcggccaagt tgctgggcct gccttacccc ggcgggccgc acctggccaa
     2461 actggcgctg ggcggcgacg cccaggcctt caagttgccc cggccgctgc tgcacagcgg
     2521 cgacctcgat ttttcatttt ccggcctgaa aaccgcagta ttgacgcagg ccaaaaaact
     2581 cggcgagccc atgggcgagc aggcccgggc cgacctggcg gcctcgacgc aggcggccat
     2641 tgtggaggtg ctggtcaaaa aatcggtgaa agcgctggag cagaccggct tgaagcggct
     2701 ggtggtggcc ggtggcgtgg gggccaatgc cttgctgcgc gaacaactca acgcggcctg
     2761 cagcaaaaag gggattcggg tgcattaccc tgagatgcat ttgtgcaccg ataacggcgc
     2821 catgatcgcg ctggcggccg gcatgcggct gcaggcaggc ctggaaagtc tgcagcgcgg
     2881 ctacaccttt gacgtgaagc cgcgctgggg tctggcggaa atcactgcct gacccggttt
     2941 gccggcggcg cagggggcta gacgcagtgg gcgaacgtgg gggccgggtt tttccgccgg
     3001 gccgccccaa ggaaaaacgc gccccctcgg ggggcagaga gcgaccgtgg gggccaggtc
     3061 ttactgaatc gtcagctgcg ccacgttgct gaccgggacc ttttttccca gcgtcaaggt
     3121 caacacgccg ttttccagtt tggcggtgct tgcagtggcg tcgatttcca caggaaaacg
     3181 gtaagccgcc ttgaccttgc gtgcggcatc ggctttgctg gcaatacgca ccacatcgcc
     3241 ttcgatgccg atgtccagct gttccctggc caggcctgga acgtccagtt gcagtgaata
     3301 ggccttgtcg ccgtcttcca ccgacgccga ttgggcgggg cggctgtcgg acagggagcc
     3361 cagggtgtca ctcaggaatt tgtcaagggc gccattgctg accagcgagg aggcgggacg
     3421 ctgaaagttt cgtgcaggac ggccggcaag tgaataaaac ataaggactc cagttggttg
     3481 ggcgggaaaa ttttcccctt acacttcgcg gctcccaatc gtgatcgccg catgcacagc
     3541 aggtaagcgt gcccaggcgt atttcaagga aattccagaa tgtatttttt cagttccagg
     3601 gtcttgaaac gtgccgtact ggccctaggc gcgagcgtct gcctgtggtc ggccagtgcc
     3661 tctgcacagc agcccgtggc ggcaccgtct cctgtgcgcg tcgcgctgat cgagagtttg
     3721 tccggaccgt ttgccaatac cggcgaagcc gtgttccgca acctggtgtg ggccgcggag
     3781 cgcgtcaatg cgcgcggcgg cctcaagctg cccggcggca atcgcccgct ggttttcgag
     3841 cgctacgaca gcaagggcca gaacgaggaa gccctgtcgg ccctgcgctc cgccctcgat
     3901 gacgggatca cgctggtgac ccagggcaac tcgtcgtcga cggccgcggt gctgatcgat
     3961 gcgatcaaca aacacaatga gcgccagccg gcgcagcgcg tgctgtacct gaattattcc
     4021 gcggtggatc cggtgctgac caatgaaaaa tgcagctact ggcacttccg cttcgatgcc
     4081 cacgccgaca tgcgcatggc cgcgctgatg gacgtactca aggacgacaa ggcggtcaag
     4141 agcatttacc tgatcgggca ggactacagc ttcggccagg ccgtgctgcg cgaagcccgc
     4201 cggcaactgg gtgcccagcg ccccgatgtg cagatcgtcg gtgacgagtt gcacccgatg
     4261 ggccgcgtca aggactttct tccctatgcc gccaagatca aggccagcgg tgcacaggcc
     4321 gtgcttaccg gcaactgggg gaacgatctg accctgctgg tcaaggccgc gagggacgtg
     4381 ggttttgaag gcagcttcta cacgttttac ggcaatgcgc tgggcgtccc cgccgcgctc
     4441 ggtgatgccg gcgtcggcaa ggtcatcgcc gtggccgact ggtttcccaa cgagccgggt
     4501 gcccagtcgg aggcgtttta ccagtccttc cgacaacgtt ttccgaaacc ccaggacgac
     4561 tatgtgcaca tgcgcatgca gctgatggtc gaggccctgg cgcagagcat cgaaaaagcc
     4621 cgtacggtgg agtcaaccgc cgtcgccgcg cagctggaaa aggcgacggt gctgtttgcc
     4681 ggccacaagg gcgtgatgcg cagcgccgac caccagttcc agcagccgct gctggtggcc
     4741 gtgatggaca agcaggggac acccggcgtc aggttcgatg tggagggctc gggttatggg
     4801 tttcgtgtga tcaagaccat cgctgcagcg cgctccgaac aggcgcacag ctgcaggatg
     4861 cagcggccct gaaccgcacg ccaacgcccc cctccgctga aagacacgca tgagacaagc
     4921 cgttttagac ctcgaagagt cgatgatccg ccaggtggcc aatgccggca tggggcgcag
     4981 cgatgtgctc aagttctggt ttggtgaaag tgacgaggtc acgcccgcct tcatccgcga
     5041 cgccgccatc acctcgctgc agcagggcga aactttttat gcgcacaacc tgggcctgcc
     5101 ggagctgcgc gaggcgattg cgcgttacgc caccggcttg cgctgcgccg gcgcggcagc
     5161 gattgatgcg gaccgcattg ccgtcacctc cggcggcgtg aatgccctga tgctggccat
     5221 gcaggcgctg gtggatgccg gcgatgaggt ggtggcggtc acgccggtct ggcccaacct
     5281 gaccgcgcag ccgctgatca tgggcgccag gctgcgctgc gtgagtctgc ggccggtggc
     5341 cggccagtgg caactcgata tggatgagtt gctggcggcc gtcacgcccc ggacccggct
     5401 gttgattgtc aacgcaccga acaacccaac cggctggacc ctgagccgcg ccgagcagga
     5461 aaccctgctc gcacactgcc gccgcaccgg tacctggatt ctcgccgacg aggtgtacga
     5521 gcggctttat ttcgagccca cggaaaatgg ctgcgcgccg agtttcctgg atatcgctgt
     5581 cccggatgac cggctggtgg ttgtgcacag tttttccaag agctttttga tgaccggctg
     5641 gcgcttgggc tggctggtga tgccgccggg catgacgccg cacattggca agctggtgga
     5701 gttcaatacc tcgtgcgccc cggtattcgt ccagcgggcc gccctggtgg cgctggcaag
     5761 gaccgatgac gtcacgccgc agctggtggc gcacctgaaa acctgccgcg acacgctggt
     5821 gccgctgctg caggccctgc cgggcgtgga agtggccccg gcccggggcg gcatgtatgc
     5881 atttttccgg ctgtcggatc aggagcgctt cggtgattcg ctggccactg ccaaacgcct
     5941 ggtggtggag gccggcctcg ggttggcccc cggcaatgcc ttcatggtca acccggcgcc
     6001 cgaggcccag ggctggctgc gctggtgttt tgcgagccgg gacgtcgcca ggctggggca
     6061 gggcgtggag cgcctggccc gctggctgcc gggcgcctga gccggcccgg agactgggct
     6121 ataatccaag gctctgccca aaagcgtgct ttttcattcg tgaaccagca cctcgcagaa
     6181 cacggcggca acggtcaact cccacg
//
LOCUS       DAIDFG010000183         6176 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_183_length_6176_cov_17.385231, whole genome shotgun sequence.
ACCESSION   DAIDFG010000183 DAIDFG010000000
VERSION     DAIDFG010000183.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6176)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183_length_6176_cov_17.385231"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1108 a         1962 c         2088 g         1018 t
ORIGIN      
        1 ccgtcggcgt tgtgcgcctg gtccgggtgg gcggtcagca cctgccgtac ctgctgcacc
       61 agcagccggt cgcggcggta ttgcagcacc gtcagcggca gggcgcgctg cagcatgtgc
      121 tgcagggctt tttcgtcgcg gcgcaggggc agggcgaggt aacgcgcatc gaagcggatc
      181 tcggcctgcg cagcatcaaa acgtatcggg ccgggaaaca tcagcccgta ggcgtcggca
      241 tgcggcggtg cggcaaacgg gaagcgcgca ccctgcagcg gaatgcggga gtcgatgtac
      301 cagcaggcca ggccgtggat gttgcggagc acgtgcacca ggcagaactc gcgcgcgcct
      361 tggccatgcc gggccaggtc ggtgcgctct tcaatcgtga tggcggcgct gtcgccggtg
      421 acgaccagct tgagggcaat gtcgcccgcc agcagggcgt ggtggcggca ccagcgtttg
      481 agggccacgc cgagatcggg cgcgctgagc gaggcccggg ccagcatgcc gtagctgccc
      541 cagggcagtt ttctcgagaa ggcgcccagg gcttcatcgt caagctcctg catggccgtg
      601 ccggacaaca cttccatctg gcgtgcggtg atgcgggcct cgagctgctg cagccgggct
      661 ggcgtgatct gtgccagctt caaggcgttg gcggggctac gaccgtaaag ttcgtaggcc
      721 gccgccatca cccgtgcaaa ggccatgggg gtggcggcag ccggctgggc cggggtagcg
      781 gtcgggaggc gggcggacat gggccgagtt tggcgcaagc tggcacgatt tgcaacctgt
      841 gtggcggccg tctggcggca tttgcgccta agctgcggta ttggcgttaa ggtgtggtcc
      901 atgcgcggcc gccggcgccg ctgtgaggaa tctatgcctg ttctggagac aaaactcaac
      961 gcccgctcgg ccgactttca ggccaatgcc gccgccatgc gcgcgctggt ggacgacctg
     1021 aagctgcagg tggccaaggc cgctgccggc ggcggtgaag cggcccgggc caaacacgtg
     1081 gcacgcggca agctgccgcc gcgcgagcgg gtgcagatgc tgctggaccc cggcacaccc
     1141 ttcctcgagc tggccccgct ggcggcgctg gccatgtacc ccgaccgcga cggcagcgac
     1201 agcgcgccct gcgccggcgt gatcaccggc ataggccgcg tcaacggtgt ggactgcatg
     1261 atcgtctgca acgacgcgac ggtgaagggc ggcacctact acccgatgac ggtcaagaaa
     1321 catttgcgcg cgcaggaagt ggcgcagcaa aaccgcctgc cctgcattta cctggtggac
     1381 tcgggcggtg ccaatttgcc gaaccaggac gaggtgtttc ccgaccgcga ccatttcggc
     1441 cgcatcttct acaaccaggc caacatgagc gcgcagggca ttgcgcagat cgcggtggtc
     1501 atgggcagtt gcacggccgg cggcgcctac gtgccggcca tgagcgacga aaccatcatc
     1561 gtgaagaacc agggcaccat ttttctgggc ggcccgccgc tggtgaaagc tgccaccggc
     1621 gagatcgtca ccgccgaaga cctgggcggc ggcgacgtgc acacccgcct gtcgggcgtg
     1681 gccgactacc tggcgcagaa cgacgcgcac gcgctggcgc tggcgcgggc tgcggttgcc
     1741 aacctgaacg caaatttcaa tagaaaagtg cctgaagtcc ttgccagtcg tgcgccacgt
     1801 gctcctgaat atgcagcaga cgaggtttac ggcgtggtgc ccaccgacac gcgcaagcct
     1861 tacgacgtgc gcgagatcat cgcccgcatc gtggacggca gcgagttcga tgagttcaag
     1921 gcgcgtttcg gcgccacgct ggtcaccggc ttcgcccaca tcgaaggcat gccggtcggc
     1981 atcattgcca acaacggcat cctgttcagc gagtcggcgc aaaaaggcgc gcacttcatc
     2041 gagctgtgtt gccagcgcaa gatcccgctg gtgtttttgc agaacatcac cggcttcatg
     2101 gtcggccgca agtacgaagc cgaaggcatt gcgcgccacg gcgccaagat ggtgatggcc
     2161 gtggccaccg cccaggtgcc caagttcacc atcatcatcg gcggcagctt cggcgccggc
     2221 aactacggca tgtgcggccg cgcctacagc ccgcgcttct tgtggatgtg gccgaacgca
     2281 cgtatctgcg tcatgggcgg cgaacaggct tcttccgtgc tggccagcgt gcggcgcgac
     2341 gcgatcgagg ccaagggcgg cagctggagc gccgaggagg aaaaagcctt caagcagccg
     2401 atgctggacc agttcgagca ccagtcgcat ccgtattact ccagtgcccg cctgtgggac
     2461 gacggcgtga tcgacccggc cgacacgcgc cgcgtgctgg cgctgggcct gcgcgccagc
     2521 ctgaacgcac cgattcccga gccgaagttc ggcgttttcc gcatgtaacg ccaccggtac
     2581 gaaaaatgtc caacgcctcc atttacgaca cgcccgagta cgagtccctg cgcgaccaga
     2641 tcgcccgctt tatcgccaag gaggtcgagc cgcacgccgc cgcctgggaa gagcagggct
     2701 ttgtgccgcg cgacgtcctc aaacgcatgg gcgccgccgg cctgttcggc ctgatgtacg
     2761 aagaaaaata cggcggtgcc gacagcgatg cgatgaccaa cctggtgttt gccgaggcgc
     2821 tgtcgcagtc cacctttgcc ggcttcatca tcaccgtgct ggtccacacc gacatggcca
     2881 gcccgcattt gcaccatgcc ggcaatccgg cgcaaaaaga aaagtacatg aagaaggtga
     2941 tcgccggcga gctgatcacc gccgtcggca tcaccgagcc gggcgccggc tcggacgtag
     3001 cgggcatacg ttccacggcg aagcgcgatg gcgacgactg ggtcatcaac ggcaccaaga
     3061 tgttcatcac caacggtgtc catgccgacc tgtactttgt ggcggccaag accggcccgg
     3121 gcaagcgcga catctcgatg ttcatcgtcg aaaaaggcac gccgggcttc accgtcggcc
     3181 gcgcgctgaa gaaaaccggc tggctgtcgt cggacaccgc cgagctgatc ttcgacaacg
     3241 tgcgcatccc cgcctggaac ctgctgggcg aggagggcaa gggcttttat tcggtgatga
     3301 agaacttcca gaccgaacgc attgcgctgg gcgcgatggc ggtcggccat tgccagcagg
     3361 cgctcaagct cacgatcgac tatgtgcgcc agcgccaggc cttcggcggg ccgctgtggg
     3421 accagcaggt gatccgccag cgcctggcca tgctggacgc gaagacgcgt gccgcacgcg
     3481 ccttcatgta ccactgcgcc tggcgcgtca cgcagggcca cgacatcgtg caggacgtct
     3541 ccatgctgaa agccctgacc ggggagctgg tcaacgaggt ggtgcagacc tgccagcagt
     3601 tccacggcgg catgggtttc atccgcgaga ccgcgatcga gcgcctgtgg cgcgacgccc
     3661 gcgtgctggc catcggcggc ggcgccaccg aagtgatgct ggaagaagtg gccaaaaggt
     3721 attgaggaag aaatgatgga acatctgcaa ctggcctgcg acggcggcgt ggccaccgtc
     3781 accctcaatc gccccgacgt tcgcaacgcg ttcaacgacg aggtcatcgc cgagatgacc
     3841 gcgtgtttcc aggacctcgg ttttcgtgac gacgtgcgct gcatcgtgct ggcggccaac
     3901 ggcacggcgt tttgcgccgg cgccgacctg aactggatga aacgcatggc cacctattcg
     3961 cgcgaggaaa acctcgccga tgccggtgcg ctggccgaga tgctgcgcgt gatctatgcc
     4021 tgccccaaac cgaccatcgc gcgtgtgcag ggcgacgtgt atgcgggcgg caccggcctg
     4081 gtggcggcct gcgacatcgc cgtgtcggtg gacacggccg gttattgcct gagtgaagtg
     4141 aagctgggcc tgattcccgc gaccatcagc ccgtatgtga ttcgcgccat gggcgcgcgg
     4201 gcgtcgcacc gctacttcct gaccgccgaa cgtttcagtg ccgcggaggc cctgcgcatc
     4261 gggtttgtgc atgaggtcgt gaatgctgac gagctcgacg ccaaggtggc cgagctggtg
     4321 aaaacgctgg tcaacgccgg cccgcaggcg gtgcggttgt gcaaaaagct ggtgcaggac
     4381 gtggcggagc aggccatcac cccggcgctg gtgcagatga cggtggaagg cattgccgac
     4441 atccgcgtca gcccggaagg ccgcgaaggt gtgcagtcct ttctgcaaaa acgcaaaccc
     4501 aattggctgt ccggtcaatc gtgaagagaa tgcaatgatc gccgcgcagg gccgccccaa
     4561 gcaagctcgc gcccccgcgg ggggcagcga ggacacgcca gtgccgagcg tgggggccgc
     4621 atgactatgg acaccgcaca gctgatagct ctcgccggcg cgctcggctg ggccagcggt
     4681 gtgcggctgt acctggtggt gctgctgacc ggcctggccg gcttcatggg ctggatcacg
     4741 ctgccgcccg gcctgcattt gctggcgcat ccggtggtgc tgggcgccag cggcttcatg
     4801 gtgttcgtcg aatttttagc cgacaagatt ccgggcctgg attcgctgtg ggacgtggtg
     4861 cacaccatga tccgcatccc cgcgggcgcg gccttggcgg ccggcgtgtt cggcgccgac
     4921 agcgggctga tggcgctgct cgcggcgctg gcgggcggca cactggcggc caccagccac
     4981 gctgccaagg cgacggcccg cgcggccatc aacacctcgc ccgaaccctt cagcaacgtg
     5041 ggcgcgtccc tgctcgaaga cggcatggtg ccgttgggcc tgtggctggc gattgcgcac
     5101 ccgctggtgt ttgtggtcgt gctgctgctc gtgctggtgg ccagcgtgtg cctgatccgc
     5161 ctttgctggc gcttcctgac gcagctgttt gcccgtgtgg cccgcatttt cagcggcaag
     5221 cccgataccg ggccgcctcc tgccttcacc ttcaaatctc ctttttcaaa gaacgactga
     5281 catgtttaaa aaaatactga ttgcgaatag aggtgaccag ccgcaaggcg gcgcagcagc
     5341 gaagctacat tgcatggcac gcgaagcgtg cgcaggcgat ttcaccgcgg gagcccacca
     5401 tgtttaagaa aatactgatc gccaaccgcg gtgaaatcgc ctgcagggtt gctgccaccg
     5461 cgcgacgcat ggcgatccag accgtcgccg tgtattccga cgcggatgcc ggcgccaaac
     5521 atgtgagcgt gtgcgacgag gccatccaca ttggcggcag cgcgcccaag gacagttatc
     5581 tgcgctggga aaaaatcatc gctgcggcga aagccaccgg tgccgaggcg attcacccgg
     5641 gttacggttt cctcagcgaa aacgaggagt ttgcccaggc ctgcggcgac gccggcctgg
     5701 tcttcatcgg cccgcccgcc tcggccatca aggccatggg cctgaaggcc gagtccaaac
     5761 agctgatgga aaaagccggc gtgccgctgg tgcccggtta ccacggcagc gaccaggacc
     5821 cggcgctgct caagcgtgaa gccgacagca tcggttaccc ggtgctgatc aaggccagcg
     5881 ccggcggcgg cggcaagggt atgcgtgcgg tcgagaagtc ggaagacttc gaggccgcgc
     5941 tggcgtcctg caagcgcgaa gccatcagca gttttggcga cgacgcggtt ctggtggaga
     6001 aatacgcgca gcgcccgcgc cacatcgaga tccaggtgtt tggcgacacc cacggcaact
     6061 atgtgtacct gttcgaacgc gactgctcgg tgcagcgccg gcaccagaaa gtgctggaag
     6121 aagcaccggc gccaggcatg accgaagcaa tgcgtcgcga gatgggtgag gctgcc
//
LOCUS       DAIDFG010000184         6165 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_184_length_6165_cov_17.156137, whole genome shotgun sequence.
ACCESSION   DAIDFG010000184 DAIDFG010000000
VERSION     DAIDFG010000184.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6165)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184_length_6165_cov_17.156137"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          880 a         2000 c         2047 g         1238 t
ORIGIN      
        1 ttcattcgcg ctgcgcgcaa ggccggttac ggcggcacct tctacaacgt gtcgtttgtc
       61 ggcacgcagg cgctggccga cgagctgggc aaggacggtg cgggcgtggt ggtgtcccag
      121 gtcatgcctt caccctacaa cgcggcgcgc ccgattgcgc gcgagtttgc cgatgccgtc
      181 aaggccgccg gtaaggatgc ccaagccaat ttctccagca tggaagggta tgtggccgcc
      241 aaactgtttg tcgagggcct caagcgcgcc agcggcaagc tcagtcgcga gtcgctgatt
      301 gccggcctgg agagcctggg cagccagtcg ctgggcggct tttccgtgtc cttctcgcca
      361 accgaccatg tggcctcggc ctttgtggag ttgtccatgc tgaccggcga cggcagggtg
      421 cgcacctgag ctgcgcctgc cgcagccccc gaaaaaaacg cctgctctgc gcaggcgttt
      481 ttttttggct gagcctttgt ggatcaggcg atgctggtcg tcgcggccag cccgcgcaga
      541 aaggcctcgc agcgtgcgag ctgttccagg ctgacgaact cgtcgggctg gtgcgcctgc
      601 gtgatgctgc ccggcccgca aaccacggtg gaaatgccgg acttcttgaa gatgccggcc
      661 tcggtgccaa acgccacctg cgtggtgcgt gactcgccgc tcaggcgctg cgccagccgc
      721 gtcaccgcgt cgttggcgga acccagaaaa ctcggcactt cgcaaatcgt ctcgaagctg
      781 aaaccggcct ccggtgctat ctttttcata gctggttccg cttgccgggc ataggccagc
      841 acctcttttt gcatggcagc cgcgtcggcc gtgggcaggt cccggaattc gtagcggaac
      901 tcggcatcac gcggcaccac attgtcggca atgccgccat ggaactggcc gacgctggcc
      961 gtgctgaaag gcacatcgaa cccctcgtag cgcggctcgt tgcgctcgaa accctcggcc
     1021 atgtcgcgca gcttgcccac cacccgcgcg gccatctcga tggcgttgac cgattgcggc
     1081 gtcagcgacg agtgggcttc cttgccgcgc acgcagcacc ggtagcggta gacacctttg
     1141 tgggcgatgg ccggcaccat gctggtgggc tcgcccacaa tgcaggccag cggcgcgatg
     1201 ccggcgtctt tcatgtcggc caccagctcg cgcacgccga aacagccgac ctcctcgtcg
     1261 tagctgagtg ccaggtgaat tgcaaaaggc gccgggctgt tcaaaaaggc atccgcctgg
     1321 gccagcgcca gcgcgatgaa acttttcatg tcggcgctgc cgcgcccata aagcttgccg
     1381 tcgcgaacca gggccgacag cgggtcgacc gaccagtcct gaccgtccca gggcacggtg
     1441 tcggtgtgtc ccgacaggat cacgccggca ggtttgcctt cccccagcgt ggcaaacagg
     1501 ttggccttgg ttttgtcggc gttccaggtg atgcggctgt ccacgccgga ttttttcagc
     1561 gtgtcgcgca cgaaatggat cagctccagg ttggagttgc ggctgacggt gttcatgcgc
     1621 accagcgtct gcgcaagcgc caaggccggg gcggacagac gggaagggga cagggtgttg
     1681 gggaaagtca tgcctgattc tggcttaatt gcgcctgcgg cgggggcggt tatgcttgaa
     1741 cccgaccgcg cgcacgtggt ctatgcttgc cccgcccgac tgccccaacc gacttttctt
     1801 ccgtccatgc gtttattgct tcgcctcgtc accttgatgc gcccccacca atggctgaaa
     1861 aacgcctttg tgttcgccgg gctggtgttc agcgagacct ggggcaacgg cccgctggcg
     1921 ctggccgtgc tcaaggcctt cgccgcgttc tgctgttttt cgagcatggt ctacattctc
     1981 aatgactggc tggaccgcgc cagcgatgca cgccatccgg tcaagcgttt gcggccgctg
     2041 gccagcggtg ccgtgtcgcc cgcgctggcg ctggcgctgg cagcgctgct gctggccgcg
     2101 ggcctggtct ttgccgccgg caacgcaatc ttgctggtgc tgctgggcat ctacgttgtg
     2161 ctgaacctgg cctattcctg gcgcctcaaa catgtgcccg tggtcgatgt gtccatcatt
     2221 gcctcgggtt tcatgctcag gctgctggcc ggcaccctgg cggtggggat tccgccttcg
     2281 cgctggatgc tgctggccgg cctgtttgtg gccctgttcc tggggttttc aaaacgcaag
     2341 gccgaaacct ttcacgacga ggccgccaag cgcgccgtgc tcgatcacta cccgccggcc
     2401 ctgctggaca tgttcatggc cgtgaccatg acggccagcc tcatgaccta cggcctgttt
     2461 gccaccagcg ccgaagcgca gctgcagcac ggcaaccggc tggtctacag cgtgcccatt
     2521 gtcgtgtttg ccatgttgcg ttacgcctac caggtgcaca gggggcgcgg cgaggacgtg
     2581 tcgcgcgacc tggtgcgcga cccctggatc gtggctgcgc tggcagcctg gctgctggtg
     2641 tttctggggc gccgcttttg aagccgccgg cccggccggc gccacgcctg ccgctcaagc
     2701 cgctgctgct ggttctgggc atggccgcca cggcgtatgc ggtggccctg gtcgtattcg
     2761 gcgaaggcag cgccctggcg accctgcagc ggctggggtc gctcgcgggc ctgcaagcgg
     2821 ccgccctgtg cctgttgaac tacctgctgc gcggcctgcg ctggcggatg tggatggccc
     2881 actacggacg tgcgctcggt gtcgcgcaag gtctgcgcct gtacctggcg ggttacacct
     2941 tcacgccgac gcccggcaac atcggcgagg cggcgcgtgg cctgatgctg gcgcgccgtc
     3001 cgctgggcat ggcgcaaagc ctggcgattt ttggtgcgga acgtctggca gacctgctgt
     3061 gcctgctgct gatgtgcctg ccggtggtct ggtggctggc ggcgctgggc ggtcctgcgg
     3121 ctaaccgcct ggcgctgggc agcggcgttg ccgcactcgt tctgctggtg cttctttttg
     3181 tgttccggcg gcgcctgttg ctgcggctgt cctggctgca ggatgcctgg cagtgcctgg
     3241 cgacccgtcc cgctgtctgg ctggcgctga ccggcgtggc ctgggccgcg cagggcctgg
     3301 ccgtctggct gctgtgccgt gaggcggggc tgtccatcgg cgtgctgcag gccgccggtt
     3361 tttacgccgt cgccatggtc ggcggcgcgc tttccctgtt gcctgcaggc ctgggcggca
     3421 tggaggcgct gttgaccggc ctgctggtgc tccatggcgc cggcgcggca caggcgctgg
     3481 gcatcaccgt gctggtgcgg gtgctgaccc tgtggctggc cgtggccatc ggtgcgctgg
     3541 cgctggtcta tagtgccgcc attcgcgaca tcagtttcag ataattcttc cagcccatgc
     3601 ttgaacgtac cgccgccccg gccgcacccc ttcttccccc ttccctggtg cgctggctga
     3661 tcgcggcagg tgcgctgtcg ctggctttgc ggctgtggat ctccgccacc ttcccgatca
     3721 ccggcgacga ggcctttttc tactggtggg gcgtctaccc cgactggggg tattacgacc
     3781 atccacccat ggtcggctgg ctgattgcct tggcgcgcgc gacgctgggc gacacgacct
     3841 gggcgatacg cctgccggtc gtgctgctgc cgctggcact cggcggcctg gtcgggtggg
     3901 cgctggcgcc gctcgaccgc gtgcgcgcag cctgggccgt gctgtttttc tggctggccc
     3961 cgatcaactg gctcaacgtg ctgatcacca ccgacacgcc gctgatggcc tggtcggtgc
     4021 tgtcggtggc gctgttgctg cgcgctgagc ggcgcgagcg catggacggc acggcctggg
     4081 ggctgtacgc gctgtcgggg ctggcgctgg gctgcgcctt tctgtccaag tacttctcgg
     4141 tggtgctggg cctgacttac ttcgtgtatt tcgcggtcta ccggcgcgaa cgctgggctg
     4201 cgtttgcgct gctggtgctg tgcgccttgc ccggcccggc catcaacatc gcctacaaca
     4261 tgtcgcacgg ctggtcgaac atcatgttca acctctacaa ccgaaacgaa ggcgagagct
     4321 tcgcctggaa caagccgctg ctctaccttg ccatgctggt ctacctggtc acgccggtgg
     4381 cggtctggct ggcctggaag cagcgcgggg cgctggccgc cgcggcgcgg cagcagcggc
     4441 tgctggcctg cgtggtggtc gtgccgctgc tgttttttgc gctgctgtcg ctgaaaaaag
     4501 tggtggggct gcactgggtg ttgtcgtttt accccttcgg ttttgcgctg ctggcctttg
     4561 ccctgccgcg cgacaagctc aagacctgtg cgctgggcat ggctgcattt gcggcgctgc
     4621 atgtgctggt ggtggcaggt ctgtacgcca gttcgctgga caactggaaa aacaccaagc
     4681 tctatacgca aatcatccgc agctacaaga ccgccgagat gctgcagcag gtggcggcac
     4741 ccggcgtgga gctgatggcc agtgcctaca cgcccgcgtc catctatggc tacacgctgc
     4801 gcaagtacgt gccggtgttc ggcccgggaa atttccatgc acggcaggat gacatgctgg
     4861 tcgatttttc gctgtaccag gggaaaaccg tgcgcatcct gcgcatggac gccccgcgca
     4921 tcgaggagta tcagccttac ttcgacagcg tgcaggtgct gagtttttcg caggacggcg
     4981 tgccctttta tgcggtcgag ggccgcaact tcaactacgc cgcctacaag aacggggtgc
     5041 tcgccaccat ttacagccgt tactacaaca tcccggcctg gttgcccatg accggctgcc
     5101 cgttttgcga gcgcctttgc ggccaggtgc gatgcacgaa acagaagcaa tagaagccgc
     5161 aggcaagcct gcggtcgtcg aagttgcggc tttcgacttc gacggcaccc tgacccggcg
     5221 cgatacgctg cttccctacc tgcgccgcag cctgggttgg ccgcggtttc tgctcgcttt
     5281 gctgcgcagc agcccctggc tggccgccta cgcctgccgc ctcaccagca accaccgggc
     5341 gaaggcgcgt ttgctgcagg ccagcctggg cgggtgcagc gcagcgcaga tcgagcgctg
     5401 gtcggccgat tttgtggcac agtacctgcc ccggcaatgg caggaagaga cgctggcacg
     5461 gctgcgctgg catcagcagc agggccatgg ctgcgtgatc gtgagcgcct cgcccggcat
     5521 ttatttgcac ggggtgggtg acgtactgcg catggatgcg gtgctctgca ccgagctcga
     5581 agtccgggac ggcacgctca ccggtcgcat ggcgacgcgc aactgccacg gcgaggaaaa
     5641 agtgctacgc ctgcaggcct ggctggcggg ccgcggcgtc cctgccgcgg cggttatttt
     5701 gcatgcgtat ggcgattcgc gcggcgacgt gccgctgcta aatctggcag actatgcgca
     5761 ctaccaaggt caaccctggg tccgcccccc agctgaaaaa tagcgggtaa ggcgggcctg
     5821 atcacccaaa ggttttcatg aaagtcattg attccatcgt cacccaggcc gccggcattg
     5881 ccgccgtgcg tcgcgatatc cacgcccacc cggagctgtg ttttgaagaa gtgcgcaccg
     5941 ccgatgtggt ggcgcaaaaa ttgaccgaat ggggcattcc catccaccgc ggcatgggca
     6001 ccaccggtgt ggtcggcatc gtcaagaacg gcacctccag ccgcgccatt ggcctgcgcg
     6061 ccgacatgga cgcgctgccc atgcaggagt tcaacacctt tgaacatgcc agcaagaccc
     6121 agggcaagat gcacgcctgc ggccatgacg gccacaccgc catgc
//
LOCUS       DAIDFG010000185         6151 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_185_length_6151_cov_18.597113, whole genome shotgun sequence.
ACCESSION   DAIDFG010000185 DAIDFG010000000
VERSION     DAIDFG010000185.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185_length_6151_cov_18.597113"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1280 a         1913 c         1844 g         1114 t
ORIGIN      
        1 ccggccgtcg ccggtcaggg cgatgtgttc gcagggcagg cctgcggcaa tgatggtttg
       61 gagttcttcg ctggtcatgg tgtatcgctc actgagtatc tgtattcatt caaacagggg
      121 ccgcgggtcc ggcttcgccg gcccgcaggc gcagcgcccc tcttcttcgt gcaaggggca
      181 gcgcaccgcc gaaggcacgc gaagcgaaaa gcgtgggggt catattcagt gcctgatttt
      241 gtagccgatg cgaagcaaat tcaccgcgat gccgctgacc accagcagtg cgctgcccac
      301 gatggccaga ctcagccagg gggacacgtc gctgacaccg aaaaaaccgt aacggaagcc
      361 gtcgatcatg tagaaaaacg ggttcaggtg gctgacggtt tgccaaaatg gtggcagcga
      421 gtggatggag tagaaaacac cactgaggaa agtcatgggc atgatcacaa aattctggaa
      481 taccgccatc tggtcgaatt tttctgccca gagaccggcg atcaacccca gagcgcccag
      541 caaggccgcg ccgagcaccg caaacaccac aatccacagc ggtgccagga aactgggctg
      601 gccgaaatag acggtgacca caaacacccc caagccgacg gccagaccgc gcacgatgga
      661 agagccgaca taggcgaaaa accagtgcca gtgcgacagc gggctgagca gcacaaacac
      721 caggctgccc atgatcttgc tctggatcag cgaagaggag ctgttggcaa aagcgttctg
      781 cagcacgctc atcatgacca ggcctggcac caggaaggcc gtgtagccga ccgtgtcgta
      841 aaccttcaca tggtcctgca gcacatggcc aaaaatcagc atgtacatga cggcggtgag
      901 gatgggcgcg cctacggtct ggaatccgac tttccagaag cgcagaactt ccttgtagaa
      961 aagcgtttgc cagccggtca tgcggtcacc ccgattttct cggccatgac ctcaagaaag
     1021 acgtcttcga ggtcggcctt gcgaatctcg acgtcctcgg ccaccaggcc ggcctcgcgg
     1081 atcgcgctga ggtagtgctc gatctcgttg gcattgtgcg ctggcagctg cacgatgcgg
     1141 ccggtgatgc gcgccttgtc ggcgatcgcc ttcggcagtt cgctgtcgat cttgaacctg
     1201 agcacattcg aggaggccgc cttgagcaac tcggaagtgg tggccaaggc caccatgcgg
     1261 ccctgcttga gcatcgcaat ccggttgcac agcgcctcgg cctcttcgag gtaatgcgtc
     1321 gtcagcagca cggtgctgcc ctgcttgttg agcctggcga caaactgcca gagtgtctgg
     1381 cgcagttcca catccacgcc agcggtgggt tcgtcaagca cgatgacctg gggtttgtgc
     1441 accagtgcct gcgccaccag cacgcggcgc ttcatgccgc ccgacagctg ccgcatattg
     1501 gcgtccgcct tgtcggccag ccccagactg agcagcaact cgtcgatcca gccgtcgttg
     1561 tttttgacac cgaaatagcc ggattgaatg cgcagagcct cacgcaccgt gaaaaaggga
     1621 tcaaacacca gttcctgcgg cacgatgccg agcttgcggc gggcagcggc gtagtcggtc
     1681 accacatcgc tgccatgcac cagcacacgg ccgctgctgg cccgcgagag gcccgccaga
     1741 atgctgatca gcgaggtttt accggccccg ttggggccca gcaggccgaa aaactcgccc
     1801 tgctggatat caaagctgac attgtccagc gcccggaccc cgggaccagt ctggctgcgt
     1861 gtcgatgggt agatttttga gacggactgg aatgagatgg cgggcatgag ccggctattt
     1921 taagggctga cgcctgtttc ttccggcccg gcagcgcaaa tgtccccagc cctgcaccca
     1981 ggcaggggcc aaaaagcgcg aacctgcccg ctttcaggtt caggccgcgg cagccgctgc
     2041 tggcggaatc aactctgcga cgccatacaa ctcggccagt tcccgcagtc gcgcaggggc
     2101 accgcgcacc gagaagcgct tgcctgcggc cagcgcatga cgccggcatt ccagcaggac
     2161 cgccagcgct gaggagtcaa actgctgcaa cccaccggcg tcggccacca cttctgcggg
     2221 cccggcaaac agctcgggtt tcaggccgcg ggcaaaacca gccgctgcgg cgtgggtcag
     2281 gacaggagga agcgtcagca cggcagcatt cccgttcagg actttttctc gcccacgttc
     2341 gttttctcgc ccgcgttcga cttgttgcgc tgggacagtg cggcgatcag tccatccacg
     2401 ccctttgcag tgatttcctg tgcgaactgg gtacggtagg tttccaccat ccagatgccc
     2461 aggacgttaa ggtcgtagat tttccagccc gtgggggttt tttccatgcg gtaatccagc
     2521 tggatcggtt cactacggcc gatcacttcg gtgcgcacga tcacttcgtt gtccgttggc
     2581 gcggcccgca ggggtttgac attgacggtc tggtccttga cctgggccag cgccccggaa
     2641 taagtgcgaa ccagcaatgt cttgaactct tcctgcaggc gcttttgctg ctcgggggtg
     2701 gcttggcgcc agttccggcc cacggtcgcc gccgtcatgc gcgtgaaatt cacatgcggc
     2761 atgaccttgc catcgaccag cgcaatgacc ttcgcggtgt cgccggcctg gatggccttg
     2821 tcggccttga tgatgtcaag cacttcggtg gaggtacgct tgatcagcgc gtccggcgct
     2881 tcctcgacgg cacctgcaac agggccggcg gccagcagaa cacctgcgag gcaggccccc
     2941 acgacatggc taaaaattcg acggttcatg gctttctttc ggttcggaac acgggcctgc
     3001 cgggccgggc ggatgtccct ttggcacaac tgacaggcaa gtgcttgatt tgacagcgtt
     3061 ttccaggctt tggttcaggc ggttcagtga agttatggca ctgcgtgtaa atgttcttta
     3121 cttggccggc gccggcgaag gcgattcggg caggtcatac cgctcctcgg tatcgccgtt
     3181 ggtgtctatc accacaccgc ggcgctgcag gtaggcgtcg cgcgtgaaac tgtatttgtc
     3241 cagggccgcc tgatctagca cctccccggc gcgcaacagg ctggcgcgaa ggtctaccgc
     3301 gcgcaggaca tagagagaat tgcggacagg tacattgtcg atctgcaaca ccaggtcgcc
     3361 tttggcgtcg actacccgtg cagcagcatc acgcaccgtc gaggggccca gtacaggcag
     3421 caccaggtag ggaccggtgg ccactcccca gcgtcccagc gtcttgccaa aatcctcgcg
     3481 gtgacgctct atgcccatgg gcgtggccca gtccagcacg ccgcccaggc caatcagcgt
     3541 gttgacgctg acacgcatga agctgtcgac ggccgcttgc ggcttggcct ggagcacgtt
     3601 gttgacgaaa gaccatgcgt ccgacaggtt gccgaagaag ttgttgacgc ccgtgcgcac
     3661 cggcgagggc accgtatcct ggtacgcctg cgccaccggc ttgacgatgg cctcgtcgag
     3721 gccgtcgttg aaacgggtca cgctgcggtt gaagggttcg aacgggtcac gcgggttcgg
     3781 gccgacggcg cagccctgca gcagggcaag cgccagcagc ccggcccaca ggccggtccg
     3841 gttcattgtg attcgtggtt tcattttttt tcgttactcc ctgagggcgg tgcagtcacc
     3901 gagccagaac tgctgtccgc cgccttgctg tacaaaaact ggctgatgag attttccagc
     3961 acaaccgcgg attgcgtgct ggagatggtg tcgccggcca ccagattctt ctcgctggcc
     4021 ccggcctcga tgccgatgta ctgctcaccg agcaagccgc tggtgaggat cttcagcgaa
     4081 ctgtccttgg gaaaggcata acgactctcc atggccagcg tgacacgcgc ctggaaagac
     4141 ttgtcgtcaa acaccagtga ctccacacga ccaaccacca caccgccgct gcgcaccgcg
     4201 cccttgggtt tgaggccgcc gacgttgtca aatttggccg tgatccggta ggtggactgg
     4261 aaactcaggt tcagcagatt ggcggcctgc aatgccagaa acaggatggc cgcagcgccg
     4321 atcatgacaa aaataccaac ccacacatca ttttttgagc gttgcacaac accctcctca
     4381 ggtaaacatc atcgcggtca ggatgaaatc cagacccaga accgacaggg aagccaccac
     4441 caccgtgcgc gtcgtggcgc tggccacgcc ttccggtgtg ggttgcgcct cgaacccctg
     4501 taacagcgca ataaacgtca cggtaaagcc gaaaacaaca ctcttgatca caccgttgcc
     4561 cacatctttc cagacatcga ccccgccttg aatctggctc cagaaagacc ctgcatcgac
     4621 cccgatcatg accacgccaa cggcccaccc gccaatcacg cccatcgcgc tgaacacggc
     4681 agccagcagg ggcatgacaa tcacgccggc ccagaaacgg ggcgccagga tgcgctgcac
     4741 cggatcgacg gccatcattt ccatgacgct gatctgctcg ccggccttca tgaggccgat
     4801 ttctgcagtc agcgacgtgc cggcccgccc cgcaaacaac aaggccgtca ccactggccc
     4861 gagttcgcgc accagcgtca gggtcacgag caagcccagc gcatctgacg aaccaaaacg
     4921 ctgcaacgtg taataaccct gcaggccgaa cacgaagccg acaaacaggc cggacaccgc
     4981 aatgatgacc agcgaatagt tgcccaggaa atggatctgg tcgcgcacga ggccgaaacg
     5041 gcggaaagtc gaaggcaggg aaaggaggag ccgaaagaaa aggcgtgcgc ccagacccag
     5101 gtcggcgagt ttgctgcggg tggcaaagcc gacatccgaa ggtttgtacc agctcatggt
     5161 gccgctcctg gctggctggc aacgggcgcg aggccggggc caaaatcttc ctcaaccgac
     5221 aggccgggat agtggaactg aaccgggccg tccggcaggg cgttgacata ctggtacacc
     5281 agcggatcgg tgctttggcg aacttgctcg ggcgtcccct cggtcgcaat ttttccgttg
     5341 gccaggatga tcacatggtc ggccacggca aaggtctgct cgagctcgtg cgagacaaaa
     5401 atactggtca gacccatggc gtcgttcaag tgccggatca gccgcgccgc cgttcccagt
     5461 gaaatcgggt ccagaccgga aaaaggctcg tcatacatca ccagctcggg gtccagcgcg
     5521 atggcgcggg ccagcgcgat acgccgtgcc atgccgccgg acacctcgct gggcatgagg
     5581 tcacgcgcgc cgcgcaggcc gaccgcgttg agcttcatga ggacaatgtc gcgaatcagg
     5641 gcttcgggca ggtcggtgtg ttcgtgcagc ggaaatgcca cattctcgta aacgctcaag
     5701 tcggcaaaca gggctccgaa ctggaaaagc atgcccatgc gacgcctagc tgcatagatc
     5761 tgctcctgat ccatccgggt gacatcctgg tcatcgaaca gcaattcgcc ggattgggca
     5821 cggatctggc ctccaatgag ccgcaaaatg gtcgttttcc caccgccgga agcccccatc
     5881 agtgccgtca ccttgccgcg tggcaccgac agggaaatgt cgtccagaat gacacgctca
     5941 ccgtaaccaa aggtaaggtg acgcaattca acaagggaag gattggaaga ggaggccata
     6001 aaaagacaca agccggatat ccggcttatt gatcataagg gattactctg acccgcgcgg
     6061 gcggtcggaa tcgttcattt ttcacagaaa tcgccccgga aagatgcccg attcaggccg
     6121 ggcagcctaa ccttcatcgc gcgcgccgac a
//
LOCUS       DAIDFG010000186         6132 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_186_length_6132_cov_19.711535, whole genome shotgun sequence.
ACCESSION   DAIDFG010000186 DAIDFG010000000
VERSION     DAIDFG010000186.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 6132)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6132)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6132
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186_length_6132_cov_19.711535"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1112 a         2026 c         2018 g          976 t
ORIGIN      
        1 gccttcaaaa aaggcgatgc cccagccgtc cgtgtgttcg ccggtgttgc cgccgcgttg
       61 cgcaaaaccg ctgaagctga aggtcacgtc ggtgggcgtg ttgcagttca tgccgaggag
      121 ctggcacatg gcatcaatcc ttgacaacga gaggacgaag cctccaggct gcgaacagcg
      181 ccaacacaca cagcgccgcc agcatcatga aggactcgtt gaacgcggcc agccgcgccg
      241 ggctggtcgc agcctgcgtc agcgaatcgc catgcgccgc gagccgccat tccagcacga
      301 tgccgcacag gctcacgccg atggcgccgc ccagcatgcg cacgaagttg atggcgctcg
      361 aaccttgcga aatcaggctg tggtccagcg cccgcaagga gccgaggttg agcgacggaa
      421 ggatgaagcc gagcccgatg cggcccagga tggcccagag caccagcacc cagatggcgc
      481 tggtcagcgt caccgtcagc atcagcgcaa acgacagcgc cagcagcgcc agcccgatgc
      541 tcaccagcaa ccaggtgggg tggcggtcgg ccagccggcc caccagcgca atcgtcaccg
      601 ccagcacaaa accggcgggc agcaggatgg tgccgacata ggaggccgac aggcccagcc
      661 cgagctgcat gtagaccggc agcagatagg tggagccgaa cagcgccgtg ccgtagatga
      721 aggccacgat gctgcccatg gcgaaagtcc ggtagccgaa cagcgccagg ttcatcagcg
      781 gttcgcgccc ggtggcggcc atgcgccgct gccaggccag aaagagcacc agcgccagcg
      841 ccgcgccacc cagcagcaag gccgcttgca gcaccgagcc gccatgcagg gccaccatgc
      901 cgttgagcag gcacagggtg ccggcgccgc ccagcagcag gccgcgccag tccagtgtgg
      961 cgcccttgcg gttcggcgcc acgccgcccg gcgaggtgac cggcacatag cgccaggcca
     1021 tccacagcga ggccaggcaa aacgggatca ccatgaaaaa aatcgagcgc cagccgaaca
     1081 ggtcgaccag cacaccgccc aggctggggc cgatggccgg tgccagaacc acgcccatgc
     1141 cgaaaatgcc gctggcgcgc ccctgctcgt gcggcgcaaa agcccgcatg atgatgatgg
     1201 ccggaatcgg ctgcaccacg ccggccgcca ggccctcggc cacacgtgcg cccagcacca
     1261 gcgcaaagct gttggcaaag ccgccgacga tgccgccggc cagtagcagc agcatggtgc
     1321 ccacataggt gcggcggtag ccataccgtg ccagcagcca gggcgtggtc agcatggaga
     1381 ccgtcatcgc caccatgaag cccgaggtga cccactgcgc ccgctcctgg cccagggtga
     1441 agtgctggct catgtcggga atcgccacgt tgacgatggt cgaggacatg atggaggcca
     1501 tcgtgccgat catcaccgag agcagcagca gccagcggta acgcgcgccg taacgcgcct
     1561 gcatctcgtc cagggtggcg gggctgctca agaccggctt tgtgctgtcg cggcggaacg
     1621 aggcggcatc agtcgcgcgg ttcggaccag agcttgccgc cggtggacca gttctcgcgc
     1681 ttgatgtcgg tgatgatcac atccaccgaa tcgggcgcgc cgcccaggac gtcgacactg
     1741 acgcgggtga tttcggcgac cagctttttc ttctgctcga gggtgcggcc ttccatcatt
     1801 tcgacgtggt aggtgggcat ggttttcctt tgaattgatt tgaaccggtg ccctgatgat
     1861 aaaagcccgg cgggcgtgtg cctctttttt gtcggcaaag actgacacaa gaacaaggcg
     1921 ggcaggcccc cgggcggcgc gacaaacgtg ggaaactcca aaggaaacgg cccaccggcc
     1981 ttcgccgggc caccagccca tgcctttgcc cgccgcgcca ggcgctttga gaaaacccct
     2041 tgttcaacga cagcccttcc tccgcccccg cccatttgcc gcgtctggcc gggcgtgcct
     2101 accactgcca ttgcggcagc cccgtttttt tcaggaacag cacctgcctg gcctgcggta
     2161 cggcgctggg ttacgacccg gcgcaggcca gcctcctgcc cctgatgccg ggcgaccaac
     2221 ccgacacctg ggtggcctgg cagcccgaag acggcgctgc gcagccagag acaccgccgg
     2281 cggtttacct ccgctgcgac aacctgcaga ccccggcggc ctgcaactgg ctggtgccgg
     2341 tcagccagga cgaaccgcag ttgctgctgt gccaggcctg tcgcctcaac cgcaccattc
     2401 ccgacctgaa cgatccggcg cacccggaca acggagagct gtggggtcac atcgagctgg
     2461 ccaagcggcg cctggtgtcg gcgcttctgg tgctgggtct gccggtggcg tcgcgggcga
     2521 gtgaggacag cgaacatggc ctgatgttcg acttcctgcg ctcacccgac ttcggcccgc
     2581 acatcatgac cgggcacgac accgggctga tcaccctcaa cctcaacgaa gccgacgacg
     2641 ccacgcgcga ggccacgcgc aaggccatgc atgagcccta ccgcaccttg ctgggtcacc
     2701 tgcgccacga ggtcggccac tattactggg accgcctggt cgccggcacg tcctggcaac
     2761 ccggttttcg ggagctgttc ggcgatgaaa gccaggacta cctggccagc ctgcaggcca
     2821 actacgagca ggggccgcgg ccggactggg ccctgcacta cgtgagcgcc tacgccagcg
     2881 tgcacccgtg ggaagactgg gccgagtgct gggcccatta cctgcacatg cgcgacaccg
     2941 tcgacacggc cctgagtttt ggcctgtcgg ccgaaagcgc ccagctggaa ttcacgccgt
     3001 tcacgctgga tgccttgtac cagccggacc accccggtgc gcaggtgttc ctggacttcc
     3061 tgaaccagtg gacacgcctg accacgctgc tcaacgaaat gtcgcgcgcc atggggcagc
     3121 ccgactttta cccctttgtg ctgccgcgcg aggtggtggc caagctgcat ttcatccacc
     3181 tggtggtgac ttccgtccat gacggggcag accatggaca ggggcaggcg caaggccagc
     3241 gccaaagtca gttctgatca aaaatcggct ctagcccaat atccgtgggc gggagcagct
     3301 cctgattcaa tagcagccgg cgctgctccg gcggcggcta ttcgagcgcc tgcacggaca
     3361 ggccgtgctg ttcggaaaac gcctgaagga cggcggcaat ctgcatgtcc tgcaaggtgg
     3421 tcaccagctc gaccatcttg tggccgctgc ccttgtggct gtcgctgatg gtcaccgtgg
     3481 ccgcgccgtc gccggcggca aacagcgcct gcagcgtcgg gcccagcgcg gccatcagct
     3541 gcgcgcgttg tgcctcgggc gcgcgggtgc cggccgggta gtgcatctca aaggaaaaat
     3601 tggttttctg gctcatcctg atcttccatg tccgtcggga agtttccagt gtgccttcgc
     3661 gccggcccga tggcgtcggg caggggcgag cgatgccgtc ggcggcttgg ccccgaatga
     3721 ttgacggaca ggttctaagt atccccggcg aggtctttca tttcccaggc aatgcgcccc
     3781 agctcggctt tgagctgcag gcgccccgcg tcgtccaggc cggccaggct tttggccagg
     3841 ttcacgcggc gcggtgaaat ggccggcccg cgggaggcaa acacaatcgc gttgcttttt
     3901 tccgcggcca tgacttcgac cgcgttcccg ctgaagctgc ggtggatgcg accggccagc
     3961 accgggtagt cggggtggtc gtggtgcagg ttgaccacca gcacgccgtg ctcgcccagg
     4021 gcggcgaagc agtcgtcata aaaccgctgg gagcacagcg ccgtgggctg gccgtcctgg
     4081 tcaaaaccgt cgaccagcag cacatcgaaa accggagcgg cggcctgcag gtaaagcgcg
     4141 ccgtcggcgg cgaccactgc aaaccgttcg tcgtcgtcgg gaatctggaa ctctcgccgc
     4201 agggcgatca catgcgggtt gatctccagc acggtcatgc gcgaggcggg caactgccgg
     4261 tggcagaact tggccagcga gccgccgccg agtccgatca tgccgatgtg ggccggcgcg
     4321 ggctggaaca gcagaaaacc catcatggtc cgggtgtagt ccacgtccag ccgccaaggc
     4381 tggtgcaaaa gcatgcggct ttgcagctcg cccagcgtga aatgcagcga cttcgatgcg
     4441 ccgtcctccc gcacaaaagg cacgatgtac tgcagcgact gaaaatcgtc gggcgccagg
     4501 gtcacagcga cctcgcgatc ttgcgcaggt ccatcgcgac ggccggcagg gccatcgcca
     4561 ccagcagcag ggcaattggc agcgtggcgc tgaagcccat ggccttgaag gcgacagcgc
     4621 caaaaatgcc gcccgaaaaa aacagcccca gcagggtggc atggatgaac agcttgtcgg
     4681 tgttggcctg gacaaactgg atgttgttgg cctttttcga ctggttccag tagatcaggc
     4741 gccccagctc aatgcccagg tcggtgacga ctccggtcat gtgggtggtg cggatctcgg
     4801 cgttcgagat tttggtgatg atggcgtttt gcaggcccat gatgaaacac agcagcagca
     4861 ccgccgtcgg caccagcagg gtggcaaatg atttcaggtt ggcgcccacc agaccgaaca
     4921 tcagcagcag caccgcttcc agcagcaggg ccagcgcgta cttgctgcgc agttcctggc
     4981 gccttgccca gctgatgagc aaggtggtgg tgatggcccc gccgataaac gcggccaggc
     5041 tcaccaggcc ggccagcgcc aggccaatgc tgccgaccac caggtcgtcg gcgatggacg
     5101 agacgatgcc cgtcatgtgc gaggtatagc gctgcacggc gagaaagccg ccggcattga
     5161 tggccccggc cacaaacgcc aggatgccgc caaactgccg gttgctgcgg cgcgtgcgtt
     5221 ccctggcggt gagtcgaagc agaaatttga acaagatgga cctctgccat gcaagaagca
     5281 tgacagtgga cattatggtg cggagaaccg gtgccgccgg tgccgcccac aagatttttt
     5341 gacgaaatcg gcctccagtc cccaagtgat ggacgcgaga tgctattgaa tttgtagcgc
     5401 gtcgggtttg acctccggat gggccggcaa gccggcgtta ggcggcgcga ccttccggat
     5461 ccactggcgt ggcctccctg accaggtggt ccagcgcatg ctccacgacg ccgtgggttt
     5521 cgaccgggcg cagcagctgg tccttgatca ggcgcacgca gctgcgtttg gtcatggagt
     5581 gcggctggcc gccgtggctg acgaacatga acagggtgcc gcggttgctg gcccagatca
     5641 gctgcgcgcg cagccagctg cgtttggagt agaggtccac ccagctgcct tcgcgcaggc
     5701 ccatcaggat gtcttcgggc tggcgcccgc cagcgccggc gacgtccgcc ggggtgtcat
     5761 cggcgccgct gccgggcgcc tcagaggccg gcgtgtcgtc ctccggcgtg gcgctcagcg
     5821 ccgccggccc ggacggcagg gtgtcgtcaa agccagcctt gtcgagttcc tgcggcgcca
     5881 gccagggctg gccggcggcc ttggctttgc gctgctcggg ggtggccggc agaaggtcgc
     5941 tcaggtcgga caggtcggtt tcggccatgg ccatttccag cggtgcgttg tccgattcga
     6001 cggcgtcgcg ccggctgcgc acgcggcgca gcttgagcac cgggcggtgc agtctttcca
     6061 tggcgtcgaa aaacgcctcg gtctcgcccg ggtccagccc cagcgatgcc aggcccgcat
     6121 gcagcttgcc ca
//
LOCUS       DAIDFG010000187         6026 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_187_length_6026_cov_18.690337, whole genome shotgun sequence.
ACCESSION   DAIDFG010000187 DAIDFG010000000
VERSION     DAIDFG010000187.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187_length_6026_cov_18.690337"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1015 a         1948 c         1930 g         1133 t
ORIGIN      
        1 agaatcatcc tgttggttct gctggaagca gacagattac ggcgcggtaa caaagcggtt
       61 atgtaccgga ttgcaaatcc gagtaggtcg gttcgactcc ggcccgcgcc tccatccatg
      121 ttttttgcag ccccttgcca ggggcttttt tgttcgtcag catgtccccg gcctgagtgg
      181 tgaaataggt agacacagcg gacttaaaat ccgccgcttc gtgaaaacgg gcgtaccggt
      241 tcgattccgg tctcaggcac cagcccccgg aacatcgcct cacaggccct gtcggcatgt
      301 tggtgatgcc catttcagac cgcaactggt taagatagat tccatggccc gctacaccac
      361 ccctcctttc gaaatacacg tccacggcga agtgccgttg cgtgaggatg tcggctttga
      421 tcagctgcag gaagccctga agccgctctg gaaatacgcg ggcgccaagt cgctggcaga
      481 cggtgccgcc agctcctacg aggaagagcc cggcatacgc tttgatgtcc accagcacct
      541 gctgcaactg tgctggacgg ttcccggaga cgaggatttc cggcaggtgc ttgacgaagt
      601 ctgcatggcc ctgaacgaac tggctgccag cggtgcgccc ctcgaagtca ctttctacga
      661 tgcggatttt gacgaggatg aggaacccga tggcgaagag gcccgggacg actttgcgat
      721 gtattttgtc gggcccacgc cagctgccat catgcaggtt cagcgtgatc tgctggtgca
      781 ggacatgatc ggcctgatgg agcgtcattt cgatgcctcc gaactcggcg atgtggtgtc
      841 cgccgtcgac aagttgttcg agcagcgatt tgatgcgctg gtcagctcca tgcaactggg
      901 caggcctccc cgcgggatgg gcggccccca gggtggcgcc ggccacggcg gtggcggccg
      961 caagccgcgg catctgcatt aagccgttct aggacggagc cgcctcgtcc ttgcccgtgc
     1021 tggagaaccc cagcccgcgc aggtagacct ccaccacatc ccgtcccagg gacaccagat
     1081 cgaagtcggc gctttccagc aaccagctct ggatcaggcc gtcgatgagg gcgtacaggc
     1141 cctgcgccgc cgtcgcgacc ggaaccggca attccagcgc atggcgccgg gcggccgcct
     1201 gaaaaccacg ttcaacctgc gccagaaagt cacttcggct cttcaggtga cgcagccgca
     1261 ctgcctggag ctcgccgaca tattccacct tgtgggtagc cacttcgaag acacgacgcg
     1321 tctgcgggtc gctggcgata cggagcagcg cttctcccac gcaactgcga atgcgtgcca
     1381 gcgggtcggt atgcccctct tggccctcta gatcctcttg ccccagctgg ttctgaaagg
     1441 actgttccag gggcagggtg acccgttcca tcatcgcgtt gaagagatcg gccttgtcct
     1501 tgaagtgcca gtagaccgcg ccacgggtcg cccccgcccg ccgggcgatt tcctgcaggc
     1561 tggtgcgcga gacgccctgt gcctggaaca ggtgctcggc cgcatccagc agcttgtggc
     1621 gggtggccag ggcttcttcc ttggttcgtc ggaccatgct tttcctgaaa acagattttt
     1681 aagagggtgg acttatacat tcacaaatgt atgtaaacta taacactcgc aaaaaccctt
     1741 attcttttgc ctttcgtccg atggggccac gtgtccattg ccatccaggt tgccatccat
     1801 gacccccctt ttgcgcttta ctccagaaaa ttcgaggatc agcatgtcca tgtcccgtgt
     1861 caagctgccc tttgcggcgc tcccctccag caatcatcag cggctggtgg cccttgcgat
     1921 gttgtccctg cttcttgccg gctgtggcaa gggcgccagc caggcaccca ccgcgccacc
     1981 gcccgcgcag gtgggggtgg tgaccgtgac ccagggcgat gtggggctgg tgaccgaact
     2041 gcccggccgg ctcgaagcct cccgcgtggc gcaggtgcgg gcgcgcgccg ccggcatttt
     2101 gcaaaagcgg ctgtttcgtg aaggcagcga tgtgaaagca gggcagccgc tgttcagcat
     2161 tgatgccgcg ccctatgccg ccgcggtggc cagcgccaaa gccggccaag cccgtgccga
     2221 ggccaatctg gcgcaggcca cggcactggc gcagcgctac aagccgctgg tcgaggccaa
     2281 cgccatcagc aagcaggagt tcgccaacgc ggtggcagcg cagaagcagg ccgaagccga
     2341 tgttgccgtg ggcaaggcca atgtgcagac cgccagcatc aacctcggtt acgcctcagt
     2401 gacggccccc atctcgggcc gcatcggacg cgccctggtg acggaaggtg cgctggtcgg
     2461 gcagggcgac gcaacccagc ttgcggtgat ccagcagatc aaccccatgt acgtcaactt
     2521 cacgcagtcg gcctcggatg tgatgaagct tcgcgccgcc atggacagcg gcaagttcaa
     2581 gcgcgccagc ggcggcgatg cggccagcgt gcgcatcgtg ctagaggacg gcacggagta
     2641 tgcacgtccc ggtcgcttgc tgttttcgga cctgacggtc gatgccacca caggccagat
     2701 caccctgcgc gccgaggtgc ccaacccgag cggccagttg ctgcccgggc tgtttgtgcg
     2761 ggtgcgcctg gagcaggcgc aggccaccaa tgccatgacc ttgccgcagc aggccgtcac
     2821 gcgttcgggc cagggcgaca cggtcatggt ggtcgatgcc gaaggcaagg tttcgccgcg
     2881 gccggtgaag atcggttccg ccaaaggcaa ccagtgggtg attctcgagg ggctgaaaac
     2941 cggcgagcag gtcatggtcg acgggttcca gaaactgcgc cctggcgcca gcgtcaagcc
     3001 cgtaccctgg cagcctgcag gcgcagcgcc cgctggggcg gcttcagcac cggcggcacc
     3061 cactgcacca gctgcagcgt ccgccgcgcc tgctgcctcc gcagccgcca aataaggagc
     3121 gcactgcatg gccaagtttt tcattgatcg gcctatcttt gcctgggtga tcgcgctttt
     3181 catcatcgtg atgggcagcg tggccatcac gcaactgccg atctcccagt acccgccggt
     3241 cgcgccgccg tccattgtca tcaatgcctc ttaccctggc gcctcggccc agacgctgga
     3301 ggacagtgtg ctgtcggtga tcgagcgtga gatgaacggc tcacccggcc tgatctacat
     3361 ggagtcggtg gcccaggcca acggcaccgg cagcatcacc atcagtttcc agcccggcac
     3421 caatgccgac ctggcccagg tggacgtgca gaaccgcctg tcgcgcgcat cgccgcgcct
     3481 gccgcaggcc gtgacgcagc agggcgtgcg tgtcgacaag tcgcgctcga acttcctgat
     3541 gttcaccatg ctgtcgtcga ccaacccggc cttcgatccg atcgcgctgg gcgactacgc
     3601 gtcacgcaac gtcgtgccgg agttgcagcg cctgcccggc atcggccagg cccagctgtt
     3661 cggctccgaa cgcgccatgc gcatctggat cgatccggcc aagctgtcgg gcttcaacct
     3721 gtcgtccgcc gaggtcaccg ccgccatccg cgcgcagaac gcgcaggtgt cgtcgggcac
     3781 catcggtgac ctgcccaacg tcgcgggcca gggcatcgcc gcgaccgtag tggtcaacgg
     3841 ccagctgtcc gatgtggcgc agtttggcaa catcgtcctg cgcgccaaca ccgacggttc
     3901 ggccgtgcgg ctcaaggacg tggcgcgcat cgagctcggc ggccaggcct atgcgacctc
     3961 ggcgcggctc aatggcaagc cggcggtcgg tatcggtctg cagctgtcgc ccagcggcaa
     4021 tgcgctggcg tccgccaagg ccgtgcgcga gaagatggcc gacctggagc ggtatttccc
     4081 gcagggcgtg agctggacca ttccctacga cagctcgcgt tttgtgcaga tctcgatctc
     4141 gcaggtcgca gtcacgctgc tcgaagccgt ggcgctggtg tttctcgtga tgttcctgtt
     4201 cctgcagaac tggcgctaca ccgtgattcc gaccatcgtc gtgccgatag cgctgctggg
     4261 aaccttcggg acgctgctgg ccttgggttt ttcgatcaac gtgctgacca tgttcggcat
     4321 ggtgctggtg atcggcatcg tggtggacga cgcgatcgtg gtggtcgaga acgtcgagcg
     4381 catcatgagc gaggaggggc tgtccccgct ggaagccacg cgcaaggcga tgcggcagat
     4441 ctcgggcgcc atcatcggcg tgaccgtggt gctgatctcg gtgtttgtgc cgctcgcctt
     4501 ttttgccggc tcgaccggca acatctaccg ccagttctcg gcggtgatgg tggcgtcgat
     4561 cggcttttct gccttcatgg cgctgtccct caccccggcg ctgtgcgcga cgctgctcaa
     4621 accggtggaa gcgggccacc accacgaaaa gcgtggtttc ttcggctggt tcaaccgcag
     4681 cttttcccgc accgccaagg ggtatgaaag catcgtcgcc cgtgtgctca agcgggccgc
     4741 gcgttacctg atcatctacg tggccatcat cgccgcggtg gcggtggtct acacccggct
     4801 gccaacctcc ttcctgcccg gcgaagatca gggcaccatg ctggtcaacg ttcagttgcc
     4861 gccgggggcg acccaggacc ggacgctggc cgtcatgcag caggtcgagg gctacatcct
     4921 caagcagccc gaagtgcaga gcatggtcgg tgtgctgggc ttcagcttct cgggccaggg
     4981 tcagaatgcg gcgctggcgt ttgtcacgct caaggactgg tccgagcggg agggtcccgg
     5041 ccagtcggcc gaggcactgg cgggccgggc cttcggcgcc ttgtccggca tccgcgatgc
     5101 gttcatcttt ccgctgagcc cgccgcccat tcccgaactg ggaacggcca gcggcttcac
     5161 tttccgtttg caggaccggg caggggccgg gcacgaggcg cttgtcaacg cacgcaacca
     5221 gatgctgggc atggccggca agagcccggt gctggcgcag gtgcgcccgg acggcctgga
     5281 agacggtccg cagttgcaga tcgacattga ccgtgacaag gccaatgcgc tgggcgtgtc
     5341 ctttgattcc atcaacacca cgctgtccac cgcgctgggc tccagctatg tgaacgactt
     5401 ccccaaccag ggccgcctgc agcgtgtggt ggtgcaggcc gatgcaccgg cgcgcatgca
     5461 gccggacgac ctgttgcgta tcaatgcgag caacagccag ggccggccgg tgccgctgtc
     5521 ggcctttgcg agcacgcgct gggtgaccgg tgcgcagcag acgatccgct acaacggcta
     5581 cccggcgatg cgtatttcgg gttccgccgc gccgggctac agctcgggcg ctgccatggc
     5641 cgagatggaa aagctggcgg gccagctgcc cgccggtttt ggtttcgaat ggaccggcca
     5701 gtcacgcgag gaaaaactcg ccggctcgca ggcgctgatt ctttacggct ttgccatcct
     5761 cgcggtgttc ctgtgtctgg ccgcgctgta tgaaagctgg accattccgc tggcggtgat
     5821 tctggtggtg ccgctgggcg tgctcggggt gctgctggcc acgttgctgc gcgcctactc
     5881 gaacgacgtg tatttccagg tcggcctgat caccatcatc ggcctgtcgg cgaaaaacgc
     5941 gatcctgatc atcgagttcg cgaaagacct ggaggcgcag ggcaagagcg cgatcgaatc
     6001 ggcgctggcc gctgcccact tgcggt
//
LOCUS       DAIDFG010000188         6025 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_188_length_6025_cov_18.409045, whole genome shotgun sequence.
ACCESSION   DAIDFG010000188 DAIDFG010000000
VERSION     DAIDFG010000188.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6025)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188_length_6025_cov_18.409045"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1193 a         1709 c         1896 g         1227 t
ORIGIN      
        1 gattgccgcg ctgtctctgg aactcgaagg cctcaggcaa cgctggcgcc gcctgaagga
       61 aaagcttgaa gaggaaaaaa gtgcggtcgc cgaaattgtg gcattgcgca agcgcatcgc
      121 cgaaggattg ggggaggacg gcagccagct ggaaggcgaa gacagcgaag acatgtctgc
      181 gtccctgaac cggctcgaga agggactgga tgcgatccag agcgacgagc ccatgattcc
      241 tgtgtgtgtt gactcaggcg ttgttgcttc cgtgatttcc ggctggaccg gcattccggt
      301 cggcaagatg ctggccgacg agttgcatac cgtcctcaac ctcaaggaaa aactggccga
      361 gcgcgtggtt gggcaggacc aggcgctgga tgcgattgcg cgacgcgtcc ggacttcccg
      421 tgctgacctg gatgacccgg gcaagccggt cggggtcttc ctgctggtcg gccccagcgg
      481 cgtaggcaag accgagactg cgtttgcact ggccgacatg atttatggcg gagaccgcaa
      541 catggtgacc gtcaacatgt ccgaattcca ggaggcccac accgtgtcca gcctcaaggg
      601 ggcgccaccg ggctatgtcg gctacggtcg tggcggcgtc ctcacagaag ctgtccggcg
      661 ccggccttac agcgtggtct tgcttgacga aatggaaaag gcgcatcccg acgtgctgga
      721 gctctttttc caggttttcg acaaaggcgt catggaagat ggcgaaggtg tccagatcga
      781 tttcaagaac acgctgattc tgctgacgtc caatgccgcc caggatgtca tcaccgatgc
      841 ctgcgaaggt gggcgacggc cggatccgga agtcctgatc gagcgcctgc ggccggtatt
      901 gatgcaacag ttttcgccgg cttttctcgg gcgactggcg attgttccct attacccgct
      961 tggcgacgag cagatacggt ccattgtgag cctcaagctg ggaaagctgg cccagcgctt
     1021 ctcccgcaac cacaaagccg agttcacctg ggacgacgcc gtggcgcaaa ccatcacggc
     1081 ccggtgtacg gaagtcgaca gtggtgcgcg taacgttgac cacatcctgt cgcatgccgt
     1141 tctgcctgag ctttcacgcc aggtactgga gcggatttcc atgtccgacg cctttgacga
     1201 ggtgcatatg ggcgtggatg cacgtgacga tttttccttt actttcggcc cgccgaaaca
     1261 ggtatagagg cgcatgcgga ctggcttcaa acagacaaac tccgcgttga cggtgaccac
     1321 accgttcggc gcagatgtgt tgttgctcga ctccttccag ggcgtggagg ccatctcgca
     1381 gcctttcagg ttttcgctgg cgatgcgttc gtccgacaca ggcctggatc cggccctgat
     1441 tgtgggcgct tcggtcacgg tcaagctcgc cttgccggct ggacctgccc ggtttttcaa
     1501 cggcatcgtg tcgcgtttca tgcacgcggg cggtgaccgc tctttctcgt cctattcggc
     1561 ggagttggtg cccagcctct ggctcctgac actcagcaga gacagggcca tctaccagaa
     1621 caagacggcg gccgacatca tcaaggcggt cctgggcgag tttggcgtga cttttgatgc
     1681 gcagctgacg gggacttatg gaagccgcga atactgcgtc cagtacgacg agactgcact
     1741 ggagttcatc tcccggttga tggaggagga aggcatcttc tatttcttca ccttcaccga
     1801 tggcgggcac accatggtgc tgggcgacag ttcggccgcc cacatcgatt gtgttgatgg
     1861 agccgctttg cgttacttcc ccgaccctgg tgggcgccgc atgatggata ccgtcaacca
     1921 gctggagtac gaaagccgcc tggtggcgca gaaatttgaa tacagtgact acaactacct
     1981 cacgcccagc accgcattgc tgaccgaatc cgcgggggcg acgggcaagg gaaagcagtt
     2041 cgaatttcca ggcaaacatg cagtgctggc cgatggcacg cagcgcgcca aggtccgctc
     2101 ggaagccagc caggtcgacg gcgcggtatg ccgcggcacg agttattgct acccgctttc
     2161 cgcaggcagg aaattcacgc tctcgggtca ttcgagaaca gccttgaaca cgacgctggt
     2221 gctgcgggca gtgacgcata cggccagcaa cgcgcagtac agcaacagct ttgaggcgtt
     2281 tgccgccacc gtgcccttcc gtccgccgcg cctgtcgccg cttccgcggg tttcgggcag
     2341 ccagacggct ctggtggtgg gacccagcgg cgaggaaata tggaccgaca cgcatgggcg
     2401 gatcaaggtg cagttccatt gggaccggat tggcgtcaag gacgaaaaca gttcgtgctg
     2461 ggtgcgcgtt tcccagtcct gggccgggct cggctggggg agcctgttcc tgccgcggat
     2521 tggacaggaa gtggtggtca gttatgtcga tggcgatccc gaccggccta ttgtcactgg
     2581 ctgcgtctac aacgccgaga acaccacgcc ggtgacgctg ccgggcatgc aaacccagag
     2641 caccatcaaa tcccgcgctt ccaagcaggg caccgccggc aatgagatgc ggatggaaga
     2701 caaaaaggat tcggaagagt tctatttcca tgcgcagaaa gacatgaaga tcgagatcga
     2761 gaacgacctg agcaccaagc tggtcaaggg ttccgagaca cacacgctgg aaaagggtga
     2821 ccgcactgtt gatgtccaga cgggcaagga agtgcacaag gtcaaaggca cacgcgagct
     2881 cacgatcacc ggcaatgaga cccataccaa caaggctgat ttcagtcagg ccgtcaccgg
     2941 gaactacgaa ctgaaggtta cgggcaacct ggtgattgat gtgacaggct ccatcacgat
     3001 caagtctgga accactttcg ctgccgaggc cggcacgtcc ctgaccagca aggcaggcac
     3061 agcgctgacc aacgaggccg gcacgtccct gaccaacaag gccggactgg acctgacgaa
     3121 ccaggctggc accggattaa agaacaaggc cgggaccacg ctggacaacg agggtgcgat
     3181 ggtgaccaac aaggcgtcag ccatgcaaac ggtggacggc ggcgggatgc tgaccctcaa
     3241 gggtggcctg gtaaagatca actgagatga cggcccaaaa aacctctgcc actgctgcgg
     3301 tacccgcgga cacgcccgct ggcgagatgc cgcgcggctt tgtcgagcgc gagcagttca
     3361 atgacgccgg cgggctgtcc aaccgcgccg gtttgatgga cggtgtgctt cacggcgaaa
     3421 tgagctcttt tggcccagac ggcaagctgc ttgccagcgc ccaatacgat gcgggtgtgc
     3481 tccatggtgt cctcaggctt tttgatgagc atggcaatcc ggtgcaggag gcgaattaca
     3541 gccgcggctt gcagcatggc ttgacccgcg ttttcgtgaa agggcgcctg ttgtccgagc
     3601 agcggttttc gaatgggagt cttcatggcg agtcggtctc ctattcggaa gccggagagg
     3661 tcgccatgaa acagaatttt ttcagaggtg aggtggaagg cgaggtcttg ttcatgaacg
     3721 agggtgaggt ggtccgccgg tcgcagtacc gcaagggcct gctggaaggg gaaacgctgg
     3781 actacgaccg cgacggtgcg gtggtgcaga aagccagcta cagaaacaac ctgcttgagg
     3841 ggccgctgac gcggtactgg ccggacgggc aggtgatgga ggtgctgttg taccgcgcag
     3901 gaaaaccggt tggcaaaccc aggcgtttcg acagtaaagg ggtggagttg cgtgacgacg
     3961 ccaaaaccag cctcatgaaa cgtctcgaaa aactggtgaa aggctgagca tgggcattca
     4021 ggtcgccgtg ggcgcaaccc ttcagtgcag ttttggcgtg gcccccagcg tgctgaccgt
     4081 gcttcccgcg aaccgtgtgc tcacgggtgg accggccgcg aacatcatgg accatgttcc
     4141 gtttgtcaat gtgctgccct ttggcatgtg caattgcccc tccaacccga tggtggctgc
     4201 cgctacggct gccgctctcg gcgtgttgac gcccatgccc tgcattccca tgactgccgc
     4261 accctggatg cccggttctg cgacggtatt gctgggcggg atgcctgcct tgcagaactc
     4321 ttccaagctg atgtgccagt ggggtggcgt catccagatt cttgtcccgg ggcagttcac
     4381 cgtgatggtt ccttgaacgt gaagataggt aacaaatgca atacactttg tcgctgacga
     4441 cccggatgat gaccattgcc gggctttgcc tgttgctgct ttgtgtgctc ctgttcttgc
     4501 tcggggtgga aatcggcaag aagatttctt cacccgcaga aggaactgcg cccgcagcgg
     4561 tgttaaccgt aaccccgcct tcatcagcac ccgtcgggga cgcgccttcg ccctccgttg
     4621 ccaagccatg aatcaggatt tgatgatgtc aaaaacacag tccggacagc ccttgcacag
     4681 ccagtcaact tcctggtgga aagcctgctt gtttgttctc gggctctggg gttgtgccgg
     4741 tgcagccttc gcggcgcccc ctaccgccag cgcgccggcc cctgccgctc cattggcgga
     4801 ggtggcgctg gtaccctctc cgttgcctgc tccgagcctg gtcaaggtgg ccgatggccg
     4861 actgctggcg cccagcattg cccgcatcgt caatcgggga gaactcgtcg ttgcgatgct
     4921 cggcgtggac acgcccccgt tcttttacat gaagaagggt gagctggtcg gtctggaggt
     4981 ggacctggcc aaagcgattg cgaaggagct caaggtcacc gtcaggtttg accgcagcgc
     5041 ccgcactttc aatgaggtgg ttgatgtggt tgcccgccag gaggccgacc tcggcatcag
     5101 caagctgtcg cggacactgg cgcgggccca gatgatcagc ttcaccgagc cttatatccg
     5161 cctgaaccac gccttcatcc tgaaccgggt gaagtttgcc gaactggctc gggagcggcc
     5221 cctccccgtt gttgttcgcg aattcaaggg atccatcggg gtcattgcga aatcgtcttt
     5281 ctcagactac gccagcaaaa actttccgct ttcggagctt cgcgaatttc cgacctggga
     5341 ggctgtcctc aaggcgcttg aaaaaggcca gatcatggcg gcctaccgtg atgagtttga
     5401 gatcaagcgg attttgaaaa gtgacccgac ggcgtccttg acgctgcgca cggtcacatt
     5461 caaggacctc gaagacaccc ttggcattgc cgtgggcatt caggatccga cgctcctggc
     5521 ctttgtgaac cagtttttgg cagagagcac cgaaaagctg acgattgaca aggtgctgaa
     5581 ggccctggac cggtaatcga ttaactaagg cgtgtgatga tcaacctgaa aaaactctat
     5641 gcatttgcgc tcaacccctg ggtggtggtg gccagcctgt cggcgggttt cgcgctgggc
     5701 atgtacatgc ctgccgtcgg gctgaagctc ggttttgtgg gcgatgtgta tgtcgatctc
     5761 ctcaagatga ttgccttgcc cttcatggtg tcggccgtga ttttcagttt gcagcgcctg
     5821 ttccgcgagg gtggggccgg cgggctgatg gctcgcgtgg cggtggtgtt cgtcgtgttt
     5881 tctattgttg tcgcgttgtt gggggcgaca acactgctgg tgatgaaacc gggttccaac
     5941 ctgtcaagtg aaaccatgca gactttcgga aagattgtcg gcaacgatct caattccagc
     6001 gacacggtca tgagccttgg gggcg
//
LOCUS       DAIDFG010000189         5999 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_189_length_5999_cov_18.476952, whole genome shotgun sequence.
ACCESSION   DAIDFG010000189 DAIDFG010000000
VERSION     DAIDFG010000189.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189_length_5999_cov_18.476952"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1000 a         1986 c         1989 g         1024 t
ORIGIN      
        1 gtccaacctg caggctcagg gcacgatccg cattgaatac ggcggtttgc gggtgctgga
       61 cctggaggcg ctgcgcagca gcattattca gtaaataagg gctccggccc ttgtcctgat
      121 tgtgcaaata gctatcaaat gcatagcaaa taggcgcctc gccgcaagcg gcgctcaccg
      181 gctggtggcg ccggcctgag cgataccact ctaaaatggg tccttcatgg ctgaacccac
      241 ttcccccaaa cctcctgtct ccggcgcggc gatgcgcatt cccaaggcgg tggccgcccc
      301 gggcgccccg gcgctgacgg tccgtctgaa caaacgcatg gccgaactcg gcctgtgttc
      361 acgccgcgag gccgatgact ggatttcgcg cggctgggtc cgggtggacg gccagccggc
      421 ggtcatgggc cagccggtgg cggccaatgc gcgcatcgac gtggcgcgcg aagccgagca
      481 gtaccagcgc cagcaggtca ccatcctgat caacaagccg gtcggttatg tcagcggcca
      541 ggccgaagac gggcacgagc ctgcggtggt gctggtgcag ccgcaaaacc gctggcgcga
      601 gtgcaacagc cgcatgcgct ggggccatga gcaattgcgc ggtctggcgc cggcggggcg
      661 gctcgacatc gattcgatcg ggctgctggt gctcacgcag gacggccgcg tcgcgcggca
      721 gctgatcggc gaggactccg agatcgaaaa agaatacctg gtgcgcgtca gctacggtgc
      781 cgaaaccgtc aacgtccagg ccgtgtttcc gccggaaaaa ctcgccttgt tgcgccacgg
      841 cctgagcctg gacggtgagc ccctgaagcc ggcgcgcgtc gagtggcaaa acccggagca
      901 actgcgcttc gtgctcaagg aaggcaagaa gcgccagatc cgccgcatgt gcgagcaggt
      961 cgggctgttt gtgacgggcc tcaaacgggt gcgcattggc atggtcaacc tcggccatct
     1021 gccggtcggc cagtggcgct acctggcgcc gcacgagcgg ttttaatcct ggattgctcc
     1081 tgtttcagga gctgcttgct cccgtccctc ttgggctaaa gcccgttttc attcatcaac
     1141 tcacgcggaa acgctgcggg tggacgccgc caccggcgac cgcgacaatg gccgcatgac
     1201 caccccatcg tcgcgccgcc ccggtcgggc ccccctggct gactggcgcc gccacctgca
     1261 cctgtccgac atccggggtc tcgcccaact cgccacccag ggcacgctgg gcatcaccga
     1321 gctggtcgag aaagtgcagg gcaatgtcta caaggtggtc gccgcaccgt ttggcgcggc
     1381 cgggcagaaa tttgtggacc gcacggcggg cgcctcgggt gttcgcaaaa gcggcatcac
     1441 cggcctggtt tacggcagcg tcaagggcgt gacgcgactg gccggcggca ccgtcgatgc
     1501 cctgttgtcg cgcgtggcgc ccagggcgga gccgctagcc tcgtcgccgc agcgcgaagc
     1561 cctgctggcg gcgctcaatg gcgtactcgg cgaccaactg ctggacaccg ccaacccgct
     1621 ggccatccgc atgagcctgc gccatgacgg ccagtccctg cccctggaca aagtcgccct
     1681 ggcgcagcac ctgccgcgcg cgacaggcaa gatcctgctg ctggtgcacg gcctgtgcat
     1741 gaacgacctg cagtggcgcg ctgacagtcc ggccgaggcg ctgccgggcc acggccggga
     1801 ctacggggag gggctggcgc aggagcttgg ttacaccccg gtttacctgc actacaacac
     1861 cggcctgcac acctcggtca atggcgggca gttcgctgcc ttgctcgagc agctgttgca
     1921 ggcctggccg caggaggtcg aggccctgag cttgctgacc cacagcatgg gtggcctggt
     1981 ggcacgcggt gcctgccaca gtgcggtagc ccagggcatg cactggccca gccggctgcg
     2041 gcacctggtt tttctgggca caccgcacca tggcgcgccg ctggagaaag tcgggaactg
     2101 ggtggacacc gtgctcggca gcaacccgct cacccggccg tttgcaaaaa tcggccagat
     2161 ccgcagcgcg ggcatcaccg acctgcggca cggcaatgtg ctgccggccg actggcaggg
     2221 cgccgaccgg tttgccggcg gtctggacac gcggcagcct ttgccgctgc cggctggcgt
     2281 cgcctgtttt gcggtggccg ccgcgctggg cgtggttccg gcggctgcaa cggccggccg
     2341 caagctcgat gatggcctcg gcgacggcct ggtatccctg tccagtgcgc tgggccaaca
     2401 tgccgacgtc gcgcgctgcc tggctttccc ggcggaccgg caatgggtgg cggccgggac
     2461 cggccacatt gccctgatac acagcctgcc ggtctaccag cagctcaggc gatggctggg
     2521 gtgagcactc aggtcgccaa ccgcagccgc atcagcatca gctcctaatt caaggctaat
     2581 tcagtccggc gaagatacag cctccaccac cacaccccgc ccgccatgga cacctttgcc
     2641 gaacgtgttg cccgcgccat cctgcttgcc gcgggtgtga gcgccgtgtt tgtcctgctg
     2701 gccctgccgc cagcggacgg tgcggcgctg ccgctgctct tgcaccacca gttcctgatc
     2761 ggcttgctgg ccgccgcgct gctgggcgcc gtgctggtgc gagccctccg gccggccgtt
     2821 gtagcggcgg ggttgctcag ccaggctggt tttgtcggca ttgcgctggc cacgccgggc
     2881 ttcagcgcaa ccaccgcctt ctacctgaat gtcgccggca tggccgccct gctgtgcgtg
     2941 ggcgcgctgt tgctgcaggc cgccaggcag caggcgcgct gggaaggcct gtcgccgcgg
     3001 cacggggagg catgatgcgg gtcctgctgg tggaagacga cgacatgatt ggccgcagcc
     3061 tgcaacaggc gctggagacc aacggctggt cggtggactg ggtgcgtgac ggtctgctgg
     3121 cgcaaagcgc gctggccgac ggcgcttatg cctgcgtgct gctggacctg ggcctgccca
     3181 agcgcgacgg tgtcgaggtg ttgcgccagg cgcgtgcccg cggggatgcc acgccggtgc
     3241 tggttctcac cgcgcgcgac ggcctggacg accgcatcgc cggactggac ctcggggccg
     3301 acgactacct catcaagccc tacgagtttc gcgagctgct ggcccgcatg cgtgcggtca
     3361 tccggcggcg cgacggcagc gcgcattcgg tgatcggcaa cgccgcggtg cagctggacc
     3421 tgaccacgcg cgaggtgctg gtcaacggcg agccctcggc gctgtcggcg cgcgagtacg
     3481 cgctgcttca cgcgctgctg gaaaggccgg gcgccatcct gtcgcgcgac cagcttgaaa
     3541 accgcatcta cggctgggga gaagaagtca tgagcaatgc ggtagacgtg ctgatccacg
     3601 gcatgcgcaa gaaactgggt gccgatgtca ttcgcaacgt gcgtggcctg ggctggcgca
     3661 tcaccagcgc gccggcagcg tctcccgcct ccggagactc ggcatgatga tccacgtttt
     3721 tccctcgggc cggctgtgtt cgctgcgccg cgcgctgctg ctgtggctgc tgccggtgtt
     3781 tttgctggtg ggggcggcgt cagccggttt cgcctattgg acctacaacc gcatggtggc
     3841 caccttcatg gacagccaga tggagctgct ggctgaatcc atggtggcgc aggagcagga
     3901 catggtgtgg cccgcgttga cgggtgagca gatccacaaa tggggcagtt atgtggtgca
     3961 ggtgttcgac gcggacggcc gagtccgctc caactcctgg ccggaattgg cgcccagcct
     4021 gcagccggca ggcgggtttc atgacatcaa ggcaaacggc gcacgctggc gcagctacac
     4081 cacgccggtg aacgaggctt cgggttcacg ccgcgtgcgg gtgttgcaaa gcggcgagtt
     4141 tcgccgccac ctggccgccg aacgggccgg tagcgcgatt ttgccggtga tcatcttgct
     4201 gccgctgtcg gcctttgtcc tctggtgcgt ggccgccatg atgtcgcgtg ccatcggaga
     4261 catcgcacgc caagccgcgg cgcaggacga aaacagcatc gccgagctgc cgctggaccg
     4321 cgtgccacag gaaattgcgc cattggttgt ttccttcaac agccttctca cgcggttgcg
     4381 cgatgcgttt gccacgcagc ggcgttttgt acaggacgcc gcgcacgagc tgcgcacgcc
     4441 catcacggcg gtgagcctgc agctggagaa cctgcgcggc gattttccgg ccggtgcgcc
     4501 ggcgcagcgt tttgcccagc tggaagccgg cgtgcagcgc gcccagcggc tggttgacca
     4561 actgctcagg ctgtcgcgcc agcagtccgt gggcgcggaa tctccgtcca ttccggtcaa
     4621 tgtgcacgag gtgctgcgcg aaagcatcaa cacgctgatt gcactggccg accagcgcaa
     4681 cattgatctc ggcctggagg aatccggctt gccttcggag gccaccatgc tgacctgctc
     4741 cagcggtgac ctgcgcagcg tggttgacaa catgatagaa aacgccctgc gttattcccc
     4801 ggaaggcggg gtggtggatg tgcgtgtcac gaagagccag gggaaactgg ccatcgaggt
     4861 ggcagacacc ggccccggca ttccgccgga gctgctggac cgcgtgttcg accggttcta
     4921 tcgcgtgccg ggaagcgatg tgcgcggcag cgggctgggc ctgtccattg cccagtcggc
     4981 cgcacaacgt tgcagcatgc gcatcagcct gcgcaatcgg gacgaccgca gtgggctggt
     5041 ggcgcgcgtc gagacggttt gagcacctgc tcatttccag ctaagacttc gctcatggtc
     5101 gcctaatttg ccccaaatac agtgaggtca cggttgaagt tggagcccga aagggccggc
     5161 acaaccgaca acccggttct caaccactca ctgaaaggtc atcatcatga acaagtctta
     5221 cgccctcatc gccatcgcta cctttgctgc catcgcttcc accggtgtgc gcgcagaaac
     5281 gcccgatccg tcctcccagt ttgcgctgca gacggaaggc aaccgcaccc gcgccgaagt
     5341 catggccgaa gccagcgcca aagtgaaaac ccacagccag gagcccgcag gttcacgtgt
     5401 ggctgccacc gtgcagtccg ccgtgcccac cgccatcgtg cgtggtgagg ctgcgcaggc
     5461 cctgcgcctg ggcaagatcc gcagcggcga gctgactggc ctggaaggct gagccggcca
     5521 tcagtcgcgg gccccggggc gcatcccgga cccgcgataa tcaggtcttg aaatatgcga
     5581 cgacgcccct aactccaggg gcgtttgttt tattccccct gattttcaag aacccacaac
     5641 atgagcaagc aaaccctctc cttccaggcc gaagtggccc agctgctgaa actggtcacc
     5701 cattcgctgt actccaaccc cgacattttt cttcgtgagc tggtctccaa cgcctcggac
     5761 gcctgcgaca agctgcgttt cgaaggcctg aacaaggccg agctgtacga ggacgcgccg
     5821 aatctcgaag tgcgtgtcag cttcgacaag gcggccaaaa ccctgaccat caccgacaac
     5881 ggcatcggcc tgtcgcagat cgaagccgtg gagcacctgg gcaccattgc caaaagcggc
     5941 acgcgcgact tcatggccaa actctcgggc gaccagaaaa acgacgcgca gctgatcgg
//
LOCUS       DAIDFG010000190         5997 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_190_length_5997_cov_19.596096, whole genome shotgun sequence.
ACCESSION   DAIDFG010000190 DAIDFG010000000
VERSION     DAIDFG010000190.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5997)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_190_length_5997_cov_19.596096"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1062 a         1918 c         1880 g         1137 t
ORIGIN      
        1 ggttttgtgc acaacggccc gctggccggg ctggtggccg tgtgtgcggg ctcggacctg
       61 atgcacccgc tgggcgcgct ggtcaccggc ggtgtcgccg gcgcggtgtt cgtgctgatg
      121 ttcacgctga cgcagaaccg ctggaagatc gacgatgtcc tgggcgtctg gccgctgcac
      181 ggcctgtgcg gcgcctgggg cggcatcgcc gccggcatct tcggcagcaa ggctctgggc
      241 ggcttgggcg gcgtgcatct cggcgcgcag ctgatcggta cggtcatggg cgtgggctgg
      301 gcgctgctgg cgggcttcgc ggtctacggc ctgatcaagg ccaccatggg gctgcgcctg
      361 agccaggaag aagagtacga aggcgccgac ctgtcgatac accgcatcgg cgccaccccc
      421 gaccgcgagg tcaactggtg atggccacga tggtgacccg ccgccgtttg ctcctgaatt
      481 tatagcattc tgcgcgcacc cctattgggc tgatggccga tttggcttat gaagttcggc
      541 gaacccgctg cagggccggc gccagccagc ggtccagcga ccacggcccc gcaccgtaaa
      601 tggccagccc cgccagcagc gtgccccaga acacatgctg ctgcagggcg gccggcgcga
      661 tctcgctgag cgagatcacc gcgacggcat tgaccacgaa caggcccagc accagcagca
      721 cgggcagcac cagttcaccg gccgtcccca tgagggccgc cgccgcaggg ggcaacaggg
      781 gcacgtggta ctcgtcggtg aacagggcca cggtggtgtc ccagtcgcgc agcttggtca
      841 ggccggacag aaaaaagacc tgcgcaatgt acaggcgcgc cagcaaggcc gccgccggct
      901 gcagtgtatt gagggtccgg gtcagcccgc cccacgcgtt caggccgcgc gccagccagg
      961 ccgtacgacc attacttgtc atcatcatgt gtttccttcg gtggttggca atgaatgcac
     1021 gcccatgacc aggccggtct gcaccgcctc gcccagccag gcattgaaat cgaagggcac
     1081 ggcatccgga gcatcctccg acgcctccag ggcgtcgagc aggggccggc cggccatcag
     1141 cgcctcgagc agccggtgtt cagtccccgt gctcacgcgc acgcggggcc gcaagccctg
     1201 gcgccagacc agcacgtgtt cagcacagcc ctccgccagc ctttgtccgg cctgttgcag
     1261 gctgggactg cccgtgaggt gggcagtaac gatgctggcc accggataag ggctcgccag
     1321 caaggccacg cccgcgccca gggtgagcgt gatccctgcc ggatcctccg tggccagcca
     1381 tgcaaacgac gccgaatcgg gcggcacatc ggccgcgctg ccggccaggt gcatgcgcca
     1441 ctccagatgc gcgacatcgg cgaggtaggg ctcatcagcc agctgggcgg acgccccgat
     1501 gaagccgcca agcaggtccc cccagcaagc catgtcgccg taaaggggcg gatggatggc
     1561 ccagaagtgg cgcgccagcg cgacaaaatt ttcctcgccg accaattgca ccagcaccgg
     1621 gtaggcggcg gccagtgcac gttccgccag cgcctggccg ttcgcgcggt aggcttgcag
     1681 gccgcgttgc gagcgccgcg cctgggcggc atccccggca gacagccagc gtgccaggcc
     1741 ggcgccatcg gtcgcgccca gcaccagctg cagcagggcc tgctgctcct gctggaagct
     1801 gtcgggcctc atgctgccac cgcgctgccc gccagggctt gcagcgcgcg ggcgctggac
     1861 gcttcgtcga gcaagacctg cagcggtgga atgtcggtat cccactccac gagggtggcc
     1921 aggccgcccg gcgccttgcc aaaacgcgcc agcgcatgcc ggtaaagctg ccagacctcg
     1981 tcgcacacgc ggctgccgtg gtcatcgatg acgatgtcac cacagtcctt gtggcccgcg
     2041 aggtgcagct ccgccaccgc gtgcggcggt agctggtcca gccacgccat gcaggcgcgc
     2101 accgggtcgt ccgtgttgcc cgccagccgc tcgttgcatg cgttcacata aatattgttg
     2161 acgtcgacca gcagcccgca gccgctgcgg ctggcgagtg ccgaaagaaa atcgggttca
     2221 ctcatgtcgg aggtgttgaa gtgcacatag gcggacaggt tttcgaccag caggcggcgc
     2281 ttcagccggt cctgcacctg ctgcacattg gcgcacaaca catccagcga ctgccggtga
     2341 aaggcaagcg gcagcagatc tgccgcatgc acggtggcgc ccccccacgg tccgcgcgca
     2401 aacgaggcat ggtccgagac gcgaaccggc tcgatgcgct cgaccagcgc agcgagctgt
     2461 tccaggtgcc agggatcaat cccaactgcg gagcccagtg ccagcccgac gccatgcagg
     2521 ctgatcgggt agcgctggcg ggcctcatgc aggacagcga gcgccgcgcc gccgcgtgca
     2581 aaaaagtttt cggaatggac ttccaggaag tccagctccg gtggtgcctc gagcaggcag
     2641 gcatagtgcg gatgccgcca acctatgccc gtggtgaaaa aagtggaatc agtcacagcc
     2701 agaaggcgac gcctgtccag ggacaggctg cttcgcccgg atgggaacag tcaacaagcc
     2761 atcagctctt cttgtccatc atcggcttgt ccatcatggc cttgcctttc atcttcgcct
     2821 ggtcgaccgt catgcctttc atgccggcgc aagtgccttt ggccacgtat ttccattcac
     2881 cggcatcgtc actgagcttg gactgaccgg cgcaggaatg ggtaccggcc aggttggcgc
     2941 aatcgttctg gcccgccttg gcgatgccgt aacatttttc tttttcctgg gcgacagcgg
     3001 gtgtggccat cagcatggtg gacagcaggg aagctgcagc tgcagcaatc agggcgcgtt
     3061 ggttcatttg aaatctccag ttgattcgaa caggttaaaa atcccaacca gacccgtgtt
     3121 gtttctctgt ctggttaaag cttggtctgg cgactgccgg ctttcttaca ggctttttct
     3181 caccgtgaaa aaaatgctgg cggctacagc aggtcggcaa tgcggtccca ggcttccggc
     3241 ggaatccgtc ccaggtaacg ctcgacaatt tttccggact tgtcgaccag gtagcccgcg
     3301 ggcagctgcg cgtgttggcc caggttgtcg cggtagctgc tgtcgccggc ccagagctgg
     3361 acaaaccgct gccgggtcga caccgtgcgc gagatgatgg cttcatagtc cagcacgtcc
     3421 tgcatgcgtg cgtcggtgct gacggccacc agctcgaagg gtttgccggc ccagccttcg
     3481 tagttggtcc gcagctcggg catcttgtcg cgacacaccg cgcagccggt ggaccagtac
     3541 aacaccagca ccaccttgcc cttgagcgaa gacagcctga agggtttgcc ctccaccgtt
     3601 ttgccttcaa tgtgcggcgc tgctgcggcc actcgcgcgg cagcaacact ttccgccttc
     3661 atggaggacg gcggtgtttg cgcctgcgag ggtgcagcca tccacagcgc gatgagggcg
     3721 gccacggcaa gaccagaaca cgtccggccg gaacgggaaa cgctgtgagg gccagcgggg
     3781 ggtgatgcgc gatgagcggt gtccatggtg gtgtccttgg tgggttgcag ctacaggcga
     3841 tgcaaatgac ttcaaatcat ttggtcgccg cgcagccgga atccttacgc gcctgcccac
     3901 attatttttc tgcacacccg cgctgcggtg cggctagact tgccacccat ggccaccttt
     3961 gaagagcaac tggttgaaca ccgggtctac ctcatgcgtt ttgcgaggct gcagctgcgc
     4021 aacgatgcct gggcggaaga cgccgtctcg gaaaccctgc tggcggcact gtccaagccg
     4081 caatcattcg gcaaccggtc gcaactcaaa acctggctgg tcggaattct caagcacaag
     4141 gtcatcgacc tgatccgcag ccacgcccgg gaggtctcgc ttcccgacca tggggccgac
     4201 gacgaagccg acgaactgga caagctcgcc ttccaggccg acggccactt cgccagcccg
     4261 cccggtgact ggggcgatcc acagcaaacc ctggcgcaaa gccagttttt caaggtcctc
     4321 gatgcctgcg tcgagcgctt gcccccggcc ctggggcgtt tgttcctgat gcgcgaatgg
     4381 ctggagctgt cctgcgaaga agtctgtaag gaactctcgc tcacgccgac caatctgtat
     4441 gtccagcttc accgtgcccg cctgcgtctg cgcgaatgcc tggccattca ctggtttggc
     4501 caacaagccc actgaacggc cgtcatgaaa atccagtccc ttcccctgat gcgcacctgc
     4561 aagcaagccg cggcattgct ggtggcccga gaggaccgcg ccttgcgcct gtccgatacc
     4621 gttgccttgc gcctgcacct gctggcctgc aaggcctgcc cgcaatttga aaaccagctg
     4681 atcaccatgc gactggcgat gggtcgctgg cgcaactatg cgggtgaggc aaccagcccg
     4741 gacacgcaaa acctcaagcc tgactgattc cagcgcgccg cgctgccact ccgcttggcc
     4801 caccggtgtt ccgacgaacg cggactctca caaaagagct ggccatttgt gtgagcagcg
     4861 taaaaattcg ttttttgcac tctgccacgg tgacacaccc cgaccgcgtc tccagaaaaa
     4921 tgcaagtggg tacaaacttt ttgtttgtcc catgaaaatc aacgctcttt cctcttctga
     4981 gggtctgttt tcggcgcacc gcaccgccgc ccggcgcgcc agctcctgcg ctccggggcc
     5041 aaaccggccg ccagcccagt gttcatgcgg gtccgcgcgg cctggcgatg gcaatatttg
     5101 cttgtcttcc ccgggtcggg ccgcataaca tgaagggtgt ggattcacat ccacaccctt
     5161 caggtgatcg gtcagtttcg cgcgctacag ctgtacgttt gaagattgtt gtgctttcgg
     5221 gactccatcg cttttgttat tgtgttttag aggagtccct tcatgggcaa caaactttac
     5281 gtcggcaatc tgccttactc cgtccgcgac gaagacttgc aacaatcttt cggccagttt
     5341 ggcgctgtca ccagcgctaa agtcatgatg gaacgcgaca ccggtcgctc caaaggcttt
     5401 ggctttgtgg aaatgggcag tgatgccgaa gcgcaggcag ccattgccgg catgaatggc
     5461 cagcctctgg gcggccgcag cgttgtcgtc aacgaagccc gtccgatgga agctcgtcct
     5521 ccacgcactg gcggcggctt cggcggcggt agcggtggtg gcggtggccg ttccggcggc
     5581 ggcggctacg gtggtggcgg caacgacggc ggttttcgca gcccttatgg tggcggtggt
     5641 cgttccggcg gtggcggctc tggcggtgga cgcggcggct actaagccct gacccccaga
     5701 ccctacagct taggctgaaa acagctttaa aaggcttcaa gacttcggtt ttgaagcctt
     5761 tttgcatggt ggctttttac cgctggtcgc aaaccgctcc ggattttgct ggatttctgt
     5821 tggcttttct catatatgac gcataatgcg cgggtgtggg tataaatcca tacaggttag
     5881 gtgatcggtc agtttcgcgc gctacagcgg tacgtttgaa gatttgttgt gctttcggga
     5941 ccccatcgct tttgttattg tgttttagag gagtcccttc atgggcaaca aacttta
//
LOCUS       DAIDFG010000191         5965 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_191_length_5965_cov_19.268359, whole genome shotgun sequence.
ACCESSION   DAIDFG010000191 DAIDFG010000000
VERSION     DAIDFG010000191.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191_length_5965_cov_19.268359"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          951 a         1946 c         1988 g         1080 t
ORIGIN      
        1 cccgccccgg acgaaagagg tccgtgagtg gcgctacgac gggcaagcac cggtggaact
       61 cgcgcaaggt gacgaaatgg gccgcttttt actcggctcc accgtggtga tgctgtttcc
      121 caagggaccg tgccacttca acccggactg ggcaccgggc gcagcggtga ggctcgggga
      181 gctgatggcg aactgcccgg cgccctgacg ctgacgctgc gccgcagcgt cacttcattg
      241 ctatgaatag catagctgtt tgcgcccatc ccatgagggc tggagaccat ttttacttca
      301 aagcgcaggc tttccggtta gtttggccac gccccggctg atcggtggcc aggccaggac
      361 cgcgcacgaa gcgcggcacc tgctgaaaac ccggtcgttc tgtagcctgc ggaaactctc
      421 cgtgtttacc cgccacccct gtcagagttg acaggggtat cagtgcacgc cctggacaat
      481 gcatttaaat tcaaacactg ggtgttgtgc aaacggcggc aacgggcctg ccgtgatgaa
      541 tgaacgtccc tgcagcttcc cgggaaacga ggatgcgatg agttacctca tacggttggt
      601 ggacaacacg gaatttcccg aggaacaatg ccgcctcatc gagcagcggt atgaccgagc
      661 cctggtccat cagctcggca gtccggagca ggtggcgcag gcgcgccgcg cctgggtgca
      721 ggccaagacc cgcgaggacg aggcgacgga gacggatctc tcactggaag aaaagcaggc
      781 catcgtgcgc tgggagcgtg cggaagctgc agcgcggctg ctcgctttcg agggttacac
      841 cggtgcgctg gaagatatct acatcgacgt ccgggtgtcc ccggctggct aggaccctgc
      901 cggacagtct tgtgctggat caattgcagc cgctcgacgc caacttacac tgattttttc
      961 agcatggatc gaggcctgat ggtcacaatt gctcatgtcc agcacatcac cctactgccg
     1021 cgcgccagta caccggtgtc ccgcgtgacc ggggtggacg atgccggcga caagggcgca
     1081 tcgcggtccc tcagagtctt cgtgatcgag gacgagatgg ccctgcgaaa cctgctggtt
     1141 gacgcgctcc aggagatacc cggggtggag gtgctgtttt atgccgacaa cgaagtctcc
     1201 gcaaccaact ggctctcgtc ccatgacagc gcctgggatc ttgcggtggt cgacctgtac
     1261 ctgcggcagg gcagcggcgt cggggcgctc aactggtgca tcacacgccg ggaggaccag
     1321 cgggtggtgg tgttgagcag ccagttgagc caggaggtgc gccgcaaatg cctggccctg
     1381 cgggccgacg cagtcttcga caaggccagc gagatggatg acttcctggc ttactgccgt
     1441 gccttgctgg gcccaccggt cggggcacca gcagtcacgc cccgcatgcc ctaaactttc
     1501 ggccatcgcc gccgttccgg ctgcgaatca cggacatgga agaccacacg catgagtgca
     1561 ctgtttcgcg agcaactgct ggcggccgcc gggcaggccg cctttcccgc cgaaagaacg
     1621 ctggacccgg ccaccggcga ggaggttttc gacgccgctt ccgaggccat gctggcacag
     1681 gtcttttccc tgttcgggat caccgcgttg acgccccagg acgaagatgc cgatcgggtc
     1741 atcaacaccg cctgcaccct ggccaccgag gtggcggcgc atctgcagag cctgatctcc
     1801 gccggcggtg cgctggacag gctggaagaa gccggcgact ggcacccggc ctaccgggcc
     1861 tacctcagcg ccttgtggca gggggagcgc gacgaggtgg cgcgctgcgc gcaggcgctc
     1921 aacctgtccg caggcattcc caacggatcg ctcccgctcg aagcggggcc gctcgcctga
     1981 tgtacgcagt gcgcagtatc atggcccaat gactcccttg gctggtcggc gcggcacgat
     2041 ggcagaactg aaccacaggg cgcggaactg gctggtgggc tggtggcgcc tgatccatct
     2101 cggcgcgctg atcctggtgc tggccctgtc gccgtcgagt tacgcccgcg acaaccgccg
     2161 gtcgctggcg cgccacatct atctcaacac cgcgcccaat ctgctgggtt tcacgctgat
     2221 gtgtgcgttg atcaccgtgg tcatcacgcg catcgtcatc gtcaccgcgc tcagttacgg
     2281 cctgtcgcag tacgccttgc aggtcgtcat tcgcgtgctg gtgctggaac tgattccgct
     2341 gacggccgcc atgttcgtgg ccctgcgctg tgcgattgcg agcggggtgg agctgtcgct
     2401 gctgcgccag cgtggggagc tacaggccat gcagcagcgc ggcctggacc cggttcgtca
     2461 ggaggtgctg ccacgtgtga cggccggcat ttttgccacc atcacgctgg ccgcgctgag
     2521 ctgcgtggtg gcgctggtga tggcctacct ggccagttac ggtttcacgc tatcgggcgt
     2581 ggcggtgtac acccggcttt tcggccaggt gttcaacccc gacgtgaccc tgatcttcgt
     2641 gctgaaaacc ctgtttttca gcctggccgt gtcgctgatt ccgatggcgt cggccctgta
     2701 cgacacggcc gagctgcgtt ttcgcgccag tgccgaactg cgcatgctgg tgcgcatgtt
     2761 tgcagtcatc ctgatcatcg aggtggtgtc tctcgtgggc aactactact aaccaacatc
     2821 cccaacattc cgtccggatc ctccattcct ttcaagtcat gagcgaaccc ctgccacccc
     2881 catccccgcc tgcgccgccg gagccgccgc ccggcagcag cgtggccatg ccgccggttg
     2941 ccaacctcga gttcaaggcg ttgatgctgc tggtcttcat ggcggcgctg gtggtgggct
     3001 cggcgcttta cgtgttgtat gcgcgcggcg cctttgagag cacccagaaa ctggtgttgt
     3061 tgtccgacga ctccgaaggc gtggtggtcg gcatggacat gacgttttcg ggttttccga
     3121 ttggccgcgt gcgccgcatc gagctggctg aagacggcaa ggcccgcatc atcgtggatg
     3181 tgccgaccaa ggacgcgcat tggctgcgca cgtccagtgt gttcaccctg gtgcgcgggc
     3241 tggtgggcag caccaacatc cgcgcctaca ccggcatgct caaggacccg ctgctgccgg
     3301 acggcgcggt gcgcgaagtg ctgcgcggcg acgccggcgc cgaagtgccc aagctggtgg
     3361 ccgccgccaa ggacctgatt gaaaacctca acagcctgac gagcagtggc ggcgcgatag
     3421 gtgccagcct cgccaacgtc cagaaactga cggacaggct caacggcccg ggcggcgcat
     3481 tgagcgtgtt gctgggcaac gacgccgagg ctaaaaagct gattgccacg ctggaccgca
     3541 ccaacgcctt gctgaccaaa cttgacagcc tggcggccaa gaccgacacc caggtgttcg
     3601 gcgccaaggg cgtgatgccc gaagcgcggg ccacggtggt gcagctcaac accctgctcg
     3661 gcgaagcgcg caccagcctg aaaaaagtcg acgcggtgct cgtcgaagcc caggccgtgg
     3721 gcgccaacgc ccgggccgcc accgacgacc tgggggcgct gcgcggggag gtcgagtcca
     3781 gcttgcgcaa ggtggatggc ctgatcaacg agatcaaccg caagtggccg tttgcccgcg
     3841 acacggagat caagctgcca tgagactctt ttcacttgtc ctgtccgtgc tggccctctc
     3901 gctgctggcg gcctgcagca gcggacccaa ggcccccgac tggcagatgg aggccaagac
     3961 cgcgatggaa cgcgcggttg ccgcctacct cgaaggcaat tcgcgcgtcg aggcgcagga
     4021 gttcgcgcgc gtgcgcagcg aactctccag caccggccgc agcgacctga tggcgcgggc
     4081 cgagctgctg cgttgtgcca gccgcgtggc cagcctggtg ctggaaccct gtgccgggtt
     4141 tgaggttttg cgaccggacg caccggccgc cgaacgcgcc tatgccgact acctcgccgg
     4201 ccggctgcag ccgcaggacg tggccttgtt gcctgcaccg caacgtgccg cggccgccgc
     4261 cggtgccgat gcggcagcgc tgaaagccat gagcgacccg ctgtcgcaac tggtcgccgc
     4321 cggcgtgatg tttcgcgccg gccgtgccag tccggcggtg ctgcagcagg cggtggacac
     4381 ggcttccggc cagggctggc gcaggccgct gctggcctgg ctgggcgtgc agctgcagct
     4441 cgcggaaaag ggcagtgatt cggaggcggc cgcgcgcctg cgccgccgca ttgcgctgac
     4501 gcaaaacccg gccgcaggca gcaagccttg agccgggcgc ccgtgagtcg ctgggcgatt
     4561 gccatcgccg gcatgttgtc gctggccgcg gccatgggca taggccggtt tgctttcacg
     4621 ccgctgctgc ccatgatgct cagcgacggc gtgatcgacc tgccgtcggc cagctggctg
     4681 gccagcgcca attacctcgg ttaccttgtc ggcgcgttgc tgtgcacctt ccagccctgg
     4741 atctggaaac acctgcgttt cctgccggcc gtcgccgcct cgagctgggt gcgtgcgggc
     4801 ctggtcgcca ccacgctgct gacgctgggc atggccctgc acctgccggc cgtctggccg
     4861 ctgctgcgtt ttgcggccgg tgtggccagt gcgctggtgt ttgtctacac ctcgggctgg
     4921 tgcctggcgc agctggcccg gctggatgcg ccggcattgg gcggcatcat gtacgccggc
     4981 ccgggcgcgg gcatcgccct gagcgggttg tttgccagcg ccatggtcgc gctgcagtgg
     5041 aaggctgcta ccggctggct gattttcggc gtactcgccg cggtgttgac ggccgccgtg
     5101 tggcgcactt tccaggagcg ggggcaaggg cagggcggca gtgcggccaa cgcagtgccc
     5161 agcacgccag ccaccagcca tgcgccggcc ggcaggaggc agatgctgct gctggccctg
     5221 gcttacgggc tggccggttt tggctacatc atcaccgcaa cttttttgcc ggtgatcgcg
     5281 cgcgaagcca tgccgggctc ggtctggatc gattttttct ggcccatctt cggcctcggc
     5341 gtggtgaccg gcgccttgct ggccacccgc attccggcca ccggcgactt ccgctacctg
     5401 ctggccggcg cctacacggt gcaggcgctg ggcatcgcgg ccagcgtgtt cagccccaac
     5461 ctcgccggtt tcgccatcgg cagcctgctg ctgggcattc cgttcaccgc catcaccttt
     5521 tttgccatgc aggaggtgcg gcggctgcgg ccggccgcgg ctgccagtta catgggcctg
     5581 ctgaccgcca tgtacggcat cggccagatc gccggcccgc cgctggtcgc cgtgttgctt
     5641 cggcacagcc gcagcctggg cgagggtttc acgctgtcgc tggaaatcgc cgcggcggcc
     5701 ttgctggccg gcgcgctgat gtacctgtgg atggccagcg cctacccggt gatgaaggcc
     5761 gtgcggcagc gctgaacccg gctggctggg gtcttatagc gaaaaaggcc tctagcccaa
     5821 taagcacctg cgcaggtagc tataaaaaca ggagcgaaac aggagcaaat ccgggctcag
     5881 gctctagtac tgcaacccga aaatatcgac acatgaaagc gcgccttcgc acccatggcg
     5941 gcgttgcaat ccttgcgaag gcgta
//
LOCUS       DAIDFG010000192         5943 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_192_length_5943_cov_18.320652, whole genome shotgun sequence.
ACCESSION   DAIDFG010000192 DAIDFG010000000
VERSION     DAIDFG010000192.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192_length_5943_cov_18.320652"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          992 a         1782 c         2009 g         1160 t
ORIGIN      
        1 cgttgtgggt ttccgcctgg gcagcgcggg cttgcacggc ccgaccgatg cgggtttccg
       61 tcgggtcggc cagggccgac gagaccggcc ggtccacttc gctgggcagg gacgcgcaac
      121 cggtcagcca cagacccagc atcagcgcag ccaaccagcg ccagggggca agaaacaggc
      181 gggcaggagg cggcgtacgt gggggcatga agtggttttg gacaatgctg ccactgtagc
      241 gcaggccgct gtttcaaagg gggttacacg ccccgacacc ggcgcgaaat cctgtaggtg
      301 ttttacccga tccgccggcg acctcgcgca acccgactgc taactggaca gggtctccca
      361 gcgctgcaac gccgccatca gttcttcctc gattttcgtg ttgcgggcgt tcagttcggc
      421 cgcacgtttg gcgttggtcg tgtacagcgt gccgtcaaac agctcctgct cgatcgcctt
      481 ctgctgcgac tcgagctgct ggatcagcgc cggcaagccg tcgagttctc gctgttcctt
      541 gtagctcagc ttcttttttg atagcggctc gcgcacgtcc ttgctgggct ccggcgtgtt
      601 tttgcctgta tttttggtgc tggcgggagc agcggttgcc ttgttgctgc cgatgatttg
      661 cgccgcacgt ttcgactggg tgatccagtc cgtcacgccg ccctcgtact cgcgccagaa
      721 acccgggttg tcgggcgtgc cctcgtaggc aatcgtgctg gtgaccacgt tgtcgaggaa
      781 gcgcctgtcg tggctgacca ggaagacggt gccttcgtag ttctgcagca ggtcttccag
      841 cagctccagc gtgtcgatgt ccaggtcgtt ggtgggctcg tcgagcacca gtacattggc
      901 cggccgggcg aacaggcggg ccagcagcag gcggttgcgt tcgccgccgc tcaagctgcg
      961 cacgggggag cgggcgcgct cggggctgaa cagaaagtcg gtcaggtaac tcttgacgtg
     1021 ttttttctgg ccgttgatct cgacccactc gctgcccggg ctgatgaagt cttccagcgt
     1081 cgcgtccagg tccagggcgt cgcgcatctg gtcgaagtag gccacctgca ggttcgcgcc
     1141 ctggcgcacc ttgccggggg gcagggtcac atcgggctgc aattcgccca gaatcagctt
     1201 gagcagcgtg cttttgccgg cgccgttggg gcccagcagg cccaccttgt cgccgcgcag
     1261 cagggtcgcg ctgaaaccat tgacgatgat tttggaggca cccgggtggc caaaactttt
     1321 cgtcacattg gtcagttcgg ccacgatctt gccgctcttg tcgccgctgg agacgtccag
     1381 gttgacccgg cccaccgcat cacgccgcgc cgagcggttg tcgcgcaggg cttcgagccg
     1441 gccgatacgc gccacgctgc gggtgcggcg tgcttccaca cccttgcgga tccacacctc
     1501 ttcctgcgcc agcagcttgt cggccttggc gttgatgacg gcttcctgcg ccatctgctc
     1561 ttccttttgc accaggtact gcgcaaagtt gccggggtag ctcagcagct tgccgcggtc
     1621 cagctcgacg atgacggtcg cgacctggtc caggaaggcc cggtcgtggg tgatggtgat
     1681 gatgctgccg ttgaagttga ccagcagctc ttcgagccag gcaatcgagt cgaggtccag
     1741 gtggttggtc ggctcgtcga gcagcagcac gtcgggcttg gcgaccagcg cctgggccag
     1801 ggccacgcgt tttttggtgc cgcccgagag cgagccgacg atggcgtcct tgtccaggtg
     1861 caggcggtgc agggtttcct ccacgcgctg ctcccagttc cagccgtcca gcgcctcgat
     1921 ttccgactgc agggcgtcca ggtcggtgtg ttcgtcggcc gccaggtaca gctccaccac
     1981 gtgtctggtc cttgccagtc ctgcgctggc agctatgaaa accgtagcgt gcggatccag
     2041 gctgggctcc tgcggcacgt aggcgatgcg caggttctgc tgcagctgca gcgtgccgtc
     2101 gtcgggtttt tccagacccg cgaggatttt gagcagggag gatttgcccg tgccgttgcg
     2161 gccgatcagg ccaatgcgct ggttcggttc cagcgaaaaa tctgcgtggt ccagcagtgc
     2221 gacgtgccca aaagcgagtt gagcgtcaag aagtgtaatt aatgccatgt tgtcagggat
     2281 tatccgggtg cgccatttcg catgctggtt tccgggcttt gggagatgaa ggccggatgg
     2341 ctatagtgca atggacatca gcggcgggca gccgctcgcg ccgggccagc ccggtaccac
     2401 cggacgtttt atggaactac aaatcaacgg ccaggccgtg caggtggacg ccgacccggc
     2461 catgcctttg ctgtgggtgc tgcgcgacct gctcaacatg acgggcacca agttcggctg
     2521 cggtgtggcg gcctgcggtg cctgcaccgt gcgggtggac gggcaggcgg tgcgcgcctg
     2581 tgtcaccccc gtggccaccc tggccggcag gcgcatccag accatcgagg cgctgggcac
     2641 acccgccagg ccgcatgcgc tgcagcgcgc ctggatcgcc gaacaggtgc cgcaatgcgg
     2701 ctactgccag tccggcatgt tgatggcggc ggcggccctg ctcgaaaaga cgccgaaacc
     2761 gaccgacgcc gacattgatg ccgccatgac caacatctgc cgctgcggaa cctatcagcg
     2821 cgtccgcctg gcgatcaagc gcgccgcagg catgggtgcc gcccgggcac cggtgaagaa
     2881 gcgttcatga aaagaaggac ctggctgctc agcgcggcgg gcgctgccgg tgccctgatc
     2941 gtgggctggg gcgcgttgcc gccgcgctcg cgcaccggtt ccggccggct ctggccgccg
     3001 gccgaggacc aggtggcgct caacggctgg atcaagatca ccgccgacgg ctcggtggtg
     3061 ctggccatgg cgcgcagcga gatggggcag ggcgtccaca cggccttgcc catgctggtg
     3121 gccgaagagc tggatgtgcc gctgtcgcgc gtccaccttg agcaggccgg tgccgacagc
     3181 atctacggca atgtcgcgat gctggtcgcc ggcctgcctt ttcacccctt cgagctcgag
     3241 gagcgaccgt tcaaggtcag ggcgggcgaa tgggtggtcc gcaagctggc gcgtgagctg
     3301 ggcatcaacg ccaccggcgg ctcgagcagt gtggccgacg cctggcaggt gctgcgcgcc
     3361 gcggccgcca cggcgcgcgc ctccctgctg ggggcggcgt cgctgcagtg gcgcctgccg
     3421 gtggccgagt tcaccgttag caacggcgtg atttcgcacc cgtcgggcaa atccgcgcat
     3481 tacggtgaac tggcccgttt tgccgccgcc accccgccgg gcagcgtgac actcaaggcg
     3541 cgcgaggact ggcaggtcat tggccggaac gcgccgcgca aggatgttcc cgccaaggtt
     3601 gatggcacgg cccgtttcgg gctcgatgtg cgtttgcccg gcatgcttta cgcggcgatc
     3661 cggctgtgtc ccatgctggg cggctccccc gggacgattg atgtgaacgc ggcgctggcc
     3721 atgccgggtg tggagcgctt ggtgcgcctg gctgcctatg ccggatccac cgcgggcttt
     3781 gccgtcgtgg ggaaaagctg gtggcagtgc cagcaggcgg cgcaggcggt gccggtggac
     3841 tggcgccagc gccccgccgg cgctctcgac tcgcgccaga tcgcgcgcga gctggaagct
     3901 gcgttacagg gggaagatgg cttcaccttt tacgaaaagg ggtccgtggc acaggccgag
     3961 gcgggcgccg cccgcaagct ggaggccttg taccgcgcgc cctatctggc gcacgccgcg
     4021 ctggagccca tgaactgcac ggcccaggtc agggacggca aggtgtttct ctgggtgccg
     4081 acccaggtgc cgcagatggg tgcggcgatc gcagcgcgcg tggccggcgt ggcggtggaa
     4141 gatgtcagcg tcaccgtcac cttgctgggt ggcggttttg gccgccggct ggaggtggat
     4201 tacatcgccc aggcggtccg ggtggccctg gaatgtggtg gccgtccggt gcagctgatc
     4261 tggtcccggg aagaagacac cacccacgac ttttaccggc ccatgcatgt ggccaggctg
     4321 gtcgcggcag tcgacgccca gggccaaccc gtcagcctgc gtatccggtc ggccggcgat
     4381 gccatcagtc cgcgctggct ggaacgtggc gtacccatgc tggccggccc cgtcgatacg
     4441 cccgacaaaa ccacggcgga gggcctgttt gacctgcctt acggctttgc ccaccagcac
     4501 atgtcgcatg tggccacccg aaaaagtgtc cctgtgggtt tctggcgctc cgtggggcat
     4561 tcgcacaatg cctttttttc ggaatgtttc atcgacgaac tggctttggc ggtgcagcag
     4621 gacccgctgg ccctgcgccg gcagctgctc agggatgcgc cgcgctacct ggccgtgctg
     4681 gaactggccg cgaagcagtc cggatggggt gggaaattgc ccacggggcg tgccaggggc
     4741 atcgcattgc atgaatcttt tggctccatc gtggcgcagg tggtcgaggt ctcgctgctc
     4801 gatggccagc cgcgtgtaca ccgggtggtc tgtgcgatcg actgcggcac cgtggttaac
     4861 cccggcatcg tcgcccagca aatggaaagt gccgtggtgt ttgcgctgac ggccgccttg
     4921 tacggcaagg tggacatcca cgatggcgtg gtgcaacaga ggaacttccc cgactacccg
     4981 atgttgctgc tggcgcaggc gcccctggtt gagacccatc tggtggccag tacgcggcct
     5041 cctggcggtg tgggtgaacc gggcgtgccg ccgctcgcgc ccgccgtggc taacgccttg
     5101 tttgcactga caggaacgag gttgcggtcc ctgccgctgg ttctctagtt ttatccggcg
     5161 tagcggcagg cggtcgggtt gcggtggtgt gaagccaggg gggatcaaga cgcgtttttc
     5221 aacatctttt ctcgcaaccc ccagaaaaca gtgctatagt cgatggctct gctgatgaaa
     5281 cagcgaatcg ccaaaggcgg ctccagtcag aaggcagaaa aaaagcttca aaaaagtttg
     5341 actgtgatga aaaatccggt atataatttg aggctcagct gattacagct aacgacgaaa
     5401 gcgaagccgc caagcggtga cgaaagtagt caggatcatt aaaaatttac agccgataag
     5461 tatgggcgtt tgaaggcgat tgccaggcga agtcagtgaa agctgatcag ccaggtcttc
     5521 tgaccttaaa cgctcatgga gatagaaaat gtgaaatcgt caagatttct catcaattcc
     5581 gtttttatga gtttcttccc gcagtcaaat gtggggagtt gaaaattatt gcagtgatta
     5641 aactaaagag tttgatcctg gctcagattg aacgctggcg gcatgcctta cacatgcaag
     5701 tcgaacggta gagtagcaat actcgagagt ggcgaacggg tgagtaatat atcggaacgt
     5761 gcccagtcgt gggggataac gtagagaaat ttacgctaat accgcatacg atctaaggat
     5821 gaaagcgggg gattcgcaag aacctcgcgc gattggagcg gctgatatca gattaggttg
     5881 ttggtgaggt aaaagctcac caagccgacg atctgtagct ggtttgagag aacgaccagc
     5941 cac
//
LOCUS       DAIDFG010000193         5939 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_193_length_5939_cov_17.765806, whole genome shotgun sequence.
ACCESSION   DAIDFG010000193 DAIDFG010000000
VERSION     DAIDFG010000193.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5939)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193_length_5939_cov_17.765806"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1158 a         2028 c         1797 g          956 t
ORIGIN      
        1 gcttcagcgc cgacctgagt ggcccggtgg ctgaccgcgc gattttccac accgacaacg
       61 cctattttct ggaagacgtg gaaatcgcgt cctaccgctg caagacgaac acgcagagcc
      121 acaccgcctt ccgtggcttc ggcgggccgc aaggcgtggt gctgatcgag accatcctcg
      181 gcgacatcgc gcggcatctg gccctggacc cgctggatgt gcgcatgcgc aacctgtacg
      241 gcgtggaaga caggaacacc acccattacc agatgaaggt cgaggacaac attctcgatc
      301 cgttgctatc aaaactggag ctgtctgcgc agtaccggag ccggcgcaag gccatttcgg
      361 catggaacgc aaaaagcccg gtgatcaagc gcggcatcgc catcacgccg gtcaagttcg
      421 gcatctcctt caccgccacc ttgttcaacc aggccggcgc actcgtgcat gtgtacaccg
      481 acggcagcgt gcaggtgaac cacggcggca ccgagatggg ccaggggctg aacaccaagg
      541 tcgcgcagat cgtcgccgac gaactcggcg tggcctttga cgacgtgctg gccacggcca
      601 gcgacaccag caagataccc aacgcctcgg ccaccgccgc ctcggccggc accgacctga
      661 acgcgcgcgc cgcgcagtat gccgcgcgca atgtgcgtga caacctggcg cagtttgtgg
      721 cgggcctgga cggctgcggc gccggcgccg tgtcgtttgc cggtggtgcc atcagttcgc
      781 ccaaaggcag ccggtccttc ccggaggtgg tgcgtttggc ctacgccaac cgcatccagc
      841 tctggagcga cggtttttat cgcacaccca agatccacta cgacaagacc accctcaccg
      901 gccggccgtt ttactacttc gcctacggcg ccgcctgcag cgaggtcgcc atcgacacgc
      961 tgaccggtga aagccgcgtg ctcaaggtcg acatcctgca tgacgtgggc accagcatca
     1021 acccggcgat cgacatcggc cagatcgaag gcggctttgt gcagggcatg ggctggctca
     1081 cgacggagca gctggtgtgg aatgacaagg gcctgctgag cacccacgcg cccagcacct
     1141 acaagatccc ggcgaccggc gacatccccg aacacttcaa ggtggatctc tggcccgagc
     1201 cgaaccgcga ggacaacgtg ttcggcagca aggcggtcgg cgaaccgccc ttcatgctgg
     1261 cgatcagcgt gctggaggcg ctgaaggacg cggtggcggc agcacggccg ggtgcggttc
     1321 ggctcgatgc gccagctact gccgagactg tgctgagggc gctgaaggcc tgacgcgtca
     1381 tacgacaggc catgacgatc tccaggacta cggggctact gccattgctg cagcctccaa
     1441 agaggctccc ctggagctgg gctatagcaa caaccccggg catcctcttt ccacgacgag
     1501 atcgaaactc ggcaagcttc ggtcatggct gttcaagccg cttgggcccg aacgcgattc
     1561 tgcggttatc ctgttgcaag tatctccccc catgctcatg caaactggca tcggggcacg
     1621 cgctgaaaac cgatgcaaac aattcgcagg ggaaaaaacc atgtcactca atgaactccc
     1681 tcgcgatgaa atgccaggcg ttatcgcaca actcaaagaa gctctctaca accaccaaca
     1741 atggcacagt gcattgatcc gctcgctcgt atgcaagctg ccaggcgaca agcgtgaaac
     1801 cgcccctctg gcccacatgg aatgccgctt cggccaatgg tattacagca aagcggccga
     1861 aaaactgcgt gaccaccccg gattcctcgc catgggagag gagcaccagc gcatgcaccg
     1921 cctggccggg cagctgctca tcgccgagga ggccggtcga cccattcccg gcaccgacta
     1981 cgataacttt gccaatgcgc tcgaccgcct gcggcttgaa attattggtc tggaaaggga
     2041 gctggaagac acgctgttca accacgatcc gctgaccggg gcgctcgtcc gggccggcat
     2101 attgccaaca ttgcgtgagc aacaagaact ggtgaagcgc cgtgtccagg tcaactacat
     2161 tgccatgatg gacctggatc atttcaaggc tgtcaacgat ctccatgggc accacgcggg
     2221 tgacctcgtt ttgaccgcaa cagttcagta cctgatcaag catctccgtc cctacgacaa
     2281 ggtgttccgt tacggcgggg aagaattcct tctcagcatg ccctatgccg acttgacccc
     2341 atgccatgac agggtcaatg aactccggga aggaatcgcc gcactggaaa tagacatcgg
     2401 ccaaaaagaa cccatccata tttccgcctc cttcggcttg acgctcctcg acccggacat
     2461 cccggtggaa acgtccatcg accgcaccga caaggctctg tatgcggcca aatccggggg
     2521 gcgcaattgt gtgcggatat gggattcgtc gatgtagatg cccgctgccg gaacttcaga
     2581 cccgggttcg atttcggctg cccagccggt ccggtgttgg ccgaaaacat actgaaggcc
     2641 ttgcggaaaa gcgccgcttg gctgttcaga ggtcctggtc gcgggaagtg tccgcgcgca
     2701 ggccgcgggg cggggactcg accggcgccg gcagcttgct gcgcgtcgtg ggcccgcccg
     2761 caagctccac gcgcacccgt ccgcccgccg tcctcgccgg ggttcaggcc gccagctctt
     2821 tcatcaccac atacaaatcc gccttgcccc caaaaaccaa tgcccggcag ctcaggcagc
     2881 gtgacgtaac cattcaccac cctgacgccg ttggcatcat ccagctgcgg gttatccgga
     2941 aacacctcga gcagggaacg cccgaaaatt tcctcggtgc tgtgggtggt cagcaaatag
     3001 acctcgcttg ccccaacgat cgtgaagccc atgtcgggct ttatcacgca gccatcgacg
     3061 aaaaacgcct gactcgtcaa aggctacgct gaatatgccc cgtcaaagag ccaacacggg
     3121 tcgatgacgc aacgatttgg ctcaagcgcc accgaacgcg catcctgttc agcttaggtt
     3181 cagtcgcacg cctgcaacat ggccccaagg ttcaggacag accaccgggc ctcaacccct
     3241 tgaacctttc ccaccgagca aagccgtaga gaaaagccgc agagcaaagc gcttttcttg
     3301 cccacatccc acgaaagaat cccatgaaat ccatcaccca ggtcttcctg ttgaccgcct
     3361 gctgcacgct gctgctgacc gcatgcaaca aggcagagaa cccgggcgcg acgctgactc
     3421 cggctgccag cgcagttgcc aacgtttcag atgccgacat caccatccat gtcaaatctg
     3481 cactggacaa ggaacccggt ctggcagggc tggacatcgc agtcgtcacc accaagggcg
     3541 acgttcggct cacgggtgta gtgtcaagcc aggcgcagat cgataccgcc atcagcgccg
     3601 cacgggccgc ccagggcgcc cactccatcc acgatgagtt gaccgtcaag cagtgaagta
     3661 cagccccgcg atccggtcac cctgcctgac gcggcggtgc tgactagggc ctgttcacac
     3721 tatttatgca agacctgcct gccaaaccgc cccagcccca cctccaccag accttcggga
     3781 cggcgaagca ggaacaacac atccatcccc atgcgctgtg ccatggccag cccttcattg
     3841 gcgccctcca ccagcaaggc cgtggcccag gcgtccgcct gcatgcaggt acgcgccagc
     3901 acggtgaccg atgccacggc gttgttcaca ggcgcaccgc ggcgcgcgtc catggtgtgc
     3961 gcaaggcgcg catcgcccac ctccagatag cgccggtagt cgcccgaggt ggccacggcc
     4021 agatcttcca gttcgatgac cccgtgcacc gcgcggcgcc cagcctcggg gcgttcgagc
     4081 gccaccgccc agggcacgcc gctggcctga ccgcccacgg cgcggagctc gccatccagc
     4141 gctgccagag catgccgcac gccgtgctgt tgcagcacat gggccatgcg gtccaccgcg
     4201 tatcccttgg caatgccgca caggtcgagc tgcaagggcg cacgcttgcg ggcccggcct
     4261 gcgggcctgt ccagctccag gcacccgtgc gcagtgagcg gcgtggactg gcgtgcagcg
     4321 cggatcgcct gggcgtcggg tgcatcgcgc accgcgccaa agccccaggc atcaaccagc
     4381 gccccaacgc acggatcgaa tgcaccctcg ctcaggcgat agacatcgag cgcacagtcc
     4441 agtacctcca gcatttcagc gggcagatcc acccagtcgt ccacgggcgc gcggttcagg
     4501 cgcatgaggt cgctgtccgg tttccagggc gacatctgct cgtccacctg ctccaccgca
     4561 gcggcaaggt cctggcgcaa ggccgccagg tccatggtgt tgtcggcatc gacactggcc
     4621 gaccagcgtg tgcccatggt cgggccgtgc agggtggtgc gtttgcaggg cgcagcgggt
     4681 tcagaagaca tcttcggcat accgctcctt tgctttcagc ttcgctacgc tcaactgcag
     4741 tggtgccagc accacgtcca gcgtctcggc cacgccctgc gccatggcgc gcccgccgca
     4801 gacacgcacg atggcgccct gtgccagcag accgcggagg cgctcggcgt cgcggcgcag
     4861 ggcgtcctgc acgtaaccgc cgccgtcggg cacgcgggaa aacaccgtct gcaaagtcgc
     4921 caggcgccct tcgccgagcc agcgctggat ttcggggccg aagtagaagt cccgcgccgg
     4981 atcgcgcccg ccaaagtaca ggtgcatcgg ggtccgtctg tcgttgcggc ggatgaagcc
     5041 ggccagcggt gccacgcctg tgcccgcccc gatcaacaac acaggccgcc gcgttcgtgg
     5101 cagtgcaaag ccgggattgg accggataaa ggcggtgatg ccgtcccccg tctgcaggcc
     5161 cagcaagtgc gtggaacaca gaccgtccgg catctgacgc acgcagattt ccagaaaccc
     5221 gtcttcccag ccggacgcca gcgagtagta gcgcggcacg gccgaacctg gcggcacgat
     5281 gcccacgaga tcgccggctg caaaccgcgc cagaccgtgc ccgcgcaggc gtgcgcccga
     5341 gccctgtgca ggccacgcaa atcgcaggat cgccgtggcc tgcccggtcg caccgggata
     5401 gtcctggcgc gctataagcg tgagcgtcgc ggtaggcggc acgcgcggca catgcgccag
     5461 caccaggggt tctcccagcg cctgtgccag ggcaacgccc cagcgcgcga actgctggcc
     5521 cgattgctgg tggatgcact cgagcggcag cagcgcgggc cagccctgtg cgcgcagcgt
     5581 ctgatccagc gcctcggcaa aggcacagaa ggccgggaac tgccggtcgc caaagccaag
     5641 caccgtcacc ggcacagcgc tggcgctgag tctggcgatg tgctccaggg cgtggctggc
     5701 gtgggccggg gcctggcctt cgccataggt tgcggcaagc acaaacacct gccgcgtggc
     5761 ggccgtggtt cgaaagttct ccagcgcgct ggtgtgaacg cgatggccgc cctggctcag
     5821 cgcatcctgc agcgtttggg caaaacccca ggtgctgccg ccttcgctgg caacgaagat
     5881 gagcacgtcg gcctgtgcca gcggggtgtt gcccgtgatg tgtggcgcct ggcggcgtg
//
LOCUS       DAIDFG010000194         5926 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_194_length_5926_cov_15.181059, whole genome shotgun sequence.
ACCESSION   DAIDFG010000194 DAIDFG010000000
VERSION     DAIDFG010000194.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194_length_5926_cov_15.181059"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1040 a         1910 c         1972 g         1004 t
ORIGIN      
        1 ccaaggatgc tccgacggtg ctggaagtgc gcggcgaggt ctacatgcgc cgcgaccatt
       61 tcgagtcgct caatgagcgg caacgcgaaa aaatcgcaaa aggtgcgaag ggtgaaaaaa
      121 cctttgtgaa cccgcgtaat gcggcggccg gtgccgtgcg ccagctcgac ccggcgattg
      181 ccgcgcagcg ccccctgagc ttttttgcct acggcgtcgg cgaagtcacg ccgcctgaag
      241 agggcggacc cgacttcgac actcacttcc agttgctgct gaccctcaaa tcatggggtt
      301 ttccggttgc agcccagacg cagactgcgc aaggcgcttc tgaattgata gcattccacg
      361 aaaaaatcgg tcgtgcgcgc gaccaactgc cttacgacat cgacggcgtg gtctacaagg
      421 tcaacagcct ggcgctgcag aaaaagctcg gtttcgtgac gcgcgagccg cgctgggctg
      481 tggcgcacaa ataccccgcc caggagcaga tgaccacggt gctggcgatt gacgtgcagg
      541 tcggccgcac cggcaaactc acgccggtgg ccaagctggc gccggtgttt gtcggtggcg
      601 tgacggtgac caacgccacg ctgcataacg aggacgaggc caggcgcaag gacgtgcgca
      661 tcggcgacac ggtgatcgtg cgccgcgccg gcgacgtgat ccccgaggtg gtcagtgtgc
      721 tgctcgacaa acggacgcag gacgccccgc agttcaccat gccgcgcacc tgcccggtgt
      781 gcggctcgga cgccgtgcgg gaagagggcg aagccgacta ccgctgcacc ggcggcctgt
      841 tctgcggtgc ccagcgcaag gaggccatcc tgcattacgc gcaccggcgc gcggtcgaga
      901 tcgagggtct gggcgacaag ctggtcgagc aactggtcga cgccggcgta gtccgcaccc
      961 tgccagacct gtaccgcctg ggcctaacgg cgctcgccgg cctggagcgc atggctgaca
     1021 aatccgccaa caacctgctc gaggccctcg aaaagtccaa gcagacgacc ttgccgcgtt
     1081 tcctgttcgg cctgggcatc cgccatgtcg gcgaagccac ggccaaagag ctggcgcgcc
     1141 atttcggcag cctcgacgcg gtcatggacg cgaccgagga gcaactgctc gaggttaacg
     1201 atgtcggccc catcgtcgcc cagagcatac gcaccttctt cgaccagcca cacaaccgcg
     1261 aggtagtcga gcagttacgc gcctgcggcg tcagctggcc ggagggcgaa ccggcggcac
     1321 gcgcgccgca gccgctgtcc ggcaagacct ttgtcatcac cggcacgctg ccgacgctga
     1381 gccgcgacga ggccaaggac agggtggaag ccgcgggcgg caaggtggcg ggctcggtca
     1441 gcaaaaaaac cgattttgtg gtcgccggca cggaagccgg cagcaagctg accaaggcgc
     1501 aggagctggg tgtggccatc atcgacgaag cagccctgct ggcgatgctg gcggatccag
     1561 ccgactgaac cgttcaggtt tccggcggca cagcttcgtc actgaattgt tcgggccggc
     1621 gccacagcca tgccagcaca cccgccatgc agccgatggc aagcacggcc acccagcggt
     1681 gctgcaccac caccagcatg atggcggcgc tcacgcccat gaccgccgtg gccgcccatt
     1741 tggcgcgccg gcgcaccaca ccgccgcggc gccactcgac cagcatgggt ccaaaggagg
     1801 tatggctttc cagccaggcc agcaggcgcg gcgagctgcg ggcagccgcc caggccgaga
     1861 gcaggacaaa aggcacggtg gggagcaccg gcacaaagat gccgatcacg cccagggcca
     1921 ggctcagcag cgccagaacc agcagcgtcc agcgcgtcac cagaggaagc ggggcggccg
     1981 gctcggcggg aagggccggc tggcggcggg atggttcgga cattccccat tttgccgtgc
     2041 agcgggcaac acggccgtcc cgccttcttt ctttctcttt ctgtatgctc gacgttttga
     2101 cgacagagca cacaggattt tcatgaccgt acgcgacatt ctcaaaatgg gtgacccgcg
     2161 cctgctgcgg gtggcccagc ccgtgacggg tttcgacacc gatgaactgc acctgctcgt
     2221 ggccgacatg ttcgaaacca tgcgcgccgt caacggtgcc ggcctggccg caccgcagat
     2281 cggtgtggac ctgcagctgg tgatttttgg cacggatgcg gtgaatccgc gttaccccga
     2341 cgcgcccttg gtgccgcgca cggtgctgct caatccggtc atcacgccct tgtcggacga
     2401 catggaaaac ggctgggagg gatgtctttc ggtgcccggc ctgcgcggcg tggtgccgcg
     2461 cttctcaagc atccgctaca ccggcttcga cccttacggc gatccgataa accgcacggt
     2521 ggatggcttt cacgcacggg tggtgcagca cgagtgtgac cacctgatgg gggtgctgta
     2581 cccgatgcgc atccgggacc tcacgcagtt cggctacacc gaggtgctgt tcccgggcct
     2641 ggatgcgtct gaggacgact gagtctttcc aagtggggtt tcacccggcc tgagtcgctg
     2701 ggcggtcggc cggcaggcgc gccttgccgc cggaaccgaa caccgagcgc caccaagcgg
     2761 cggaaccttc gggtggcacc ggcggcgtgg tctcgatacg ggcgtccagg tagtccagcg
     2821 tgacacggcg gcggtactgc gcgacttctt caccggccag ctcgatcagc ggccgggccg
     2881 cgccccggct ctggatcagc acctgcttgc atccggcgcc ggccttgatc agcgccgagc
     2941 gcacggccat ggcgcggcgg tcgcccatca accagaaaaa gcggtggcta ccggtgccgt
     3001 agctgtggcc ggaaaccagc acccgctgct cgggccggtc ggccagccag gccgcgtggg
     3061 cggccagcag gtcgtcataa ctctcgggca ccagccagtt gtcgggttca aaaaagacct
     3121 gacgggccag gggccggccg tcgtcgtcgc attcgcccga ggcgtgcaac tgctgctcac
     3181 gggtcaacag ggtgtgtgtc tgggcatgca tggcaagctc cttcggtgaa atgtccggtt
     3241 gcggggctgg ccaccatttt tttcctggct gccagtccgc tgttcgttca ttgtctgctt
     3301 ctggcagccc atcacaagcc tcaaacagtg atcatgtgta acatgaagaa aatcgtttaa
     3361 cctgttgatt taaataagta aatattcttc aagcatgggg cccaggatgg acggaaagcg
     3421 gccgaaccgg cctgtgactg ctggtacgct caggccccga gggcttcgag cagttcggtc
     3481 tcgatggcga tctgcgcctt gttgtgctgc agctccggcc cgtcgatcag gaaggtgtct
     3541 tccacacgtt cgcccagggt ggtgaccttg gccagttgca ggttgatgcg gtggcgggcc
     3601 agcacgcggg cgatcgaata cagcaggccc acgcgatcgc tggccgagac gctgagcagc
     3661 cagcgctggg ctttttcgtc ggggcgcagg tccacgcgcg gcgtgatcgg gaaactcttg
     3721 acgcggcgcg acacccggcc tttgcccggc ggcggcagtg ggccattttc ttcaatggcg
     3781 cggtccaggt cagcttcgac catggccacg agttcgcggt agtggtcggc cagggtcggg
     3841 ctgaccacct ggaaggtgtc cagcgcataa ccgcttgtac ctctggtggg cttggcggtg
     3901 tggaccttgg cgtccaggat gctgaaaccg gcttcctcga aataaccgca gatgcgggca
     3961 aacaggtcgg gcgcgtcagg cgtgtacacc agtacctgca ggccttcgcc ggcggccgag
     4021 cggcgcgccc gcacaataca tttggccgtg cccacatggc gcgacaggtg gcgcgcatgc
     4081 caggcaatgt cgccggcctc gtggcgcatg aagtagctga cgtccagcga gtcccacagg
     4141 gccttgtgcg cctcgaaggg ttcggcatgc agggccagga tggtcagggc ctcgcgcttg
     4201 cgggcctcga cctcggcgtc cgcatcgggc gcacgcccgc ccagcacgcg cagcgtgtag
     4261 cggtagaggt cttcgagcag cttgcctttc caggcattcc acaccttggg gctggtgccg
     4321 cggatgtcgg ccacggtgag caggtaaagc gcggtcaggt aacgctcgtt gccgacacgt
     4381 ttggcaaacc cggcaatgac ctgcggatcg ctcaggtctt ccttctgggc aatgcggctc
     4441 attgtcaggt gttcgcccac caggaactcg atcagcttgg cgtcttcggc ggcaatgccg
     4501 tgctggcggc aaaacacccg cacgtccttg cggccgaggt cggaatggtc gccgccacgg
     4561 cccttggcaa tgtcgtggaa aagggcggcg atgtacagga tccagggctt gtcccagccg
     4621 gcccccagct gcgaacacgc cgggtattca tgcgcgtgtt cggccatgaa aaagcgccgc
     4681 acattgcgca gcaccatcag gatgtgctgg tccaccgtgt agacgtggaa caggtcgtgc
     4741 tgcatctgcc cgacgatctg gcggaacacc cacaggtagc ggcccagcac cgaggtctgg
     4801 ttcatcaggc gcatcgcatg ggtgatgccc gtgggctgct gcatgatctg ccggaaagtg
     4861 tcgcggttga ccggatcgcg cctgaagcgc gcgtccatca cgccgcgtgc gttgtacagc
     4921 gcgcgcagag tgcgggccga caggcccttg acacccacgg tggtctggta gaccagaaag
     4981 gtctcaagga tggcgtgcgg gtgggccagg tagaggtcat cgctggccac ctcgatcatg
     5041 ccggccttgt tcaggaagcg ttcgttgatc ggctgcggcg cctgcccgct ggggttgagg
     5101 cgctcctcga tgttgagcaa caggatctgg ttgagctggg tcaccgcctt ggcggtccag
     5161 taaaagcgct tcatcagcgc ttcgctggcg cgcgagacca cccgtccggt gtcgctgtgg
     5221 gtcaccgttg cataaccgaa ggactcggcc accacggtct gcaggtcgaa caccaggcgg
     5281 tcctcgcgcc ggccggccag caggtgcagg cgggcgcgta tcaggctcag cagggcttcg
     5341 ttggccttga tctggcggac ctcgaaggcc gtggccagcc cgttacgcgc caggtcgtcc
     5401 caggacttgc ccagcccggc cgcgcgtgcc acccagagga tgacctgcag gtcgcgcagt
     5461 ccgcccgggg actccttgca gttgggctcc agggagtagg gcgtgtcttc gaatttgttg
     5521 tggcgctggc gctgctccag tgtcttggct acaaaaaagg cctgcgggtc cagcacgccc
     5581 tcaagctgcc tcaacagggc agcaaacatc gttctggagc ccgccagcag ccgcgcttcg
     5641 agcagcgagg tctgcaccgt cacgtccttg gcggcttcct cgacacagtc gccgaccgtg
     5701 cgcacgctgg agccgatctc caggccggtg tcccagcaac tgccgatgaa gctctcgatc
     5761 cgggtcttca gctccgcatc gctgtccacc cgggcatcgt caggcagcag cagcaggacg
     5821 tcgacgtcgg aatgcggaaa cagctcgccc cggccaaacc cgccgaccgc caccaggcag
     5881 tagccggccg ggaatccggc gcgttgccag agctggcgca aggtgt
//
LOCUS       DAIDFG010000195         5915 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_195_length_5915_cov_18.907167, whole genome shotgun sequence.
ACCESSION   DAIDFG010000195 DAIDFG010000000
VERSION     DAIDFG010000195.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5915)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195_length_5915_cov_18.907167"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          967 a         1869 c         1983 g         1096 t
ORIGIN      
        1 gccggccctg gagcaccgcg agcagcccgc cggcgccgtg gtgctggaag tgcgcgacct
       61 gagcctggcg cgggaagacc agttcggcgt ggacctgcag cacattgcgc tgacggtacg
      121 cgccggcgag gtggtcggca ttgccggcgt ctcgggcaac ggccagaagg agctgctgta
      181 tgcgctgtcg ggtgaagaca tccgtgcggc gggcgacagc atccaggtcg cggggcaggg
      241 cgtgggccgg cttggcccca tgcagcgtcg tgcgcggggc ctgcatttcc tgcccgaaga
      301 gcggctgggg cgcggtgccg tgccgacgct gagcctggcg cagaacctgc tgctcacccg
      361 aagcgatgcg gtgggccgcg gcggctggct caagctcggt gccctgcagg cgcaggcggc
      421 cggcatcatt gcacgctacc aggtcaaggc cggcggcccg caggcggcgg cgcagtcgct
      481 gtcgggcggc aacctgcaaa aattcctggt cggccgcgag atcgaggccc aacccaccct
      541 gctgattgtt tcgcaaccga cctggggggt ggacgtgggc gcttcggcgc aaatccgcgg
      601 cgagctgctc aagctgcgcg acaagggctg cgcggtgctg gtggtcagcg aagagctcga
      661 agaactgttt gacatctgcg accgccttta cgtgatggcc aagggccaga tctcaccggc
      721 cttgccgcgt gccgacgcga cggtggaact ggtcgggcag tggatgagcg ggttgtggaa
      781 cgaccatgcc gtggagggtg cccatgctca agcttgaagc ccggccgcag ccctcgaaat
      841 ggtggagcct cggctcgccg ctgctggcgc tgctgatcac ggtggtgatc ggcgtgttgc
      901 tgtttgcggc gctgggcaag gacccggtgc gcggcctgca gatctttttc tgggaaccgg
      961 tcaagtcgcc ctatgcgctg agcgagctga tggtcaaggc gaccccgctg ctgatcattg
     1021 cgctgggcct ggccgtgtgt tttcgctcca acgtctggaa catcggcgcc gaggggcagt
     1081 ttgtcatcgg cgcggtggcg gccggcggcg tggccctgct ggccgacaaa accaccgggc
     1141 cctggatagt ccccgccatc atcgcggccg gtgtgttggg tggcatggcc tgggccggca
     1201 tcacggcact gctgcgcgac cgcttcaacg ccaatgaaat cctggtcagc ctgatgctgg
     1261 tttatgtggc cgacatggtg ctcagctacc tggtgttcgg cccctggaaa gacccgcagg
     1321 gctataactt cccgcagacc aaaaccttcg aggcggtgac gcagattccg cgcctgatgg
     1381 ccggctcgcg cgtcagcatc ggcctgttga tcggtctggc cggcgcgctg gccttgtggc
     1441 ttttcctgtt tcgcacccgc gccggttttg cccagcaggt cggtggcctg gcgcccgccg
     1501 cggcgcgtta cgccggcttt tcgtcgcgca aggccttgtg ggtggcgctg ctgacctccg
     1561 gcggtgccgc cggtctggct ggcgcgctgg aggtggccgg cccgatcggc cagctcacgc
     1621 cctacgtgcc ggccggttac ggttttgccg cgatcatcgt ggcttttgtc gggcgcctgc
     1681 acccggccgg catggtgttt tcggcgatcc tgatgagcat gttctacatc ggcggagagc
     1741 tggcgcagtc gcgcctgggc ctgcccaagt cgttgaccgg ggtgttccag ggcctgctgc
     1801 tgttcagcct gctggcctgc gacacgctga tcagctaccg cgtgcgctgg acgggccgcg
     1861 ccaaacccgt catgaaaggc gcccgctgat ggactcctac gccttgctgc tggcagcgac
     1921 cctgagcgcc ggcaccgtgc tggcgattgc ttcgctgggc ctgctgatca atgaaaaagc
     1981 cggcatcgtc aacctgggcg ccgaaggcat gatgttgtgc gccgcgattg caggctttgc
     2041 cgccgtggtg cacagcggca acatctggct cggttttgca gccggcatgg cggccggcgc
     2101 cttgctcgcg gccatcttcg gtgtgctggt gatctggctc aataccaacc agtacgcgac
     2161 cgggctcgcg ctgagcctgt ttggtgcggg cttctcggcc tttgccggca tcaagtacgt
     2221 gcaggagaag ctccccgagc agggccagta cgccttgccc tggctcggtg acctgccttt
     2281 tgtcgggccg gcgctgtttc gccagcaccc catggtttac atcgccatgg cgctgacggt
     2341 cggactgatc tggtttttgt accgcacccg cgccggtctg gtgctgcgtt cggtcggcga
     2401 aagcccggag tcggcgcatg cactcggtta cccggtgcgg cgcatccgcc tggcggcggt
     2461 ggtggccggc ggcgcgttgt gcggtctggc cggtgcctat gtctcggtgg tctacacgcc
     2521 gctgtgggtg gaaggcatga ttgccggcaa gggctggatt gcgctggccc tgaccacctt
     2581 tgccacctgg cgcccggcac gggtgttgct tggtgcttac ctgttcggtg gcgtgaccat
     2641 gctgcagttt cacctgcagg ggctgggcgt ggatgtgccg ccccagttcc tgaccatgct
     2701 gccttacctg gcgaccatcg tggtgctggt gctgatttcg cgcaacccgc gctggatcag
     2761 gctgaacatg ccgacatcga ttggaaagcc tttctacccc ggttcatgat gttcccggcc
     2821 cataatctgg gtttgccacc tcgccaaccg ttaatcacaa ccgttaaccg caaccgtcgt
     2881 tacaggagat gacatgactg acttacaaaa acgctcactg ctcaagctgg ctgccctttc
     2941 cgcagttgct tccgcggccc ttatcggttg cggcaaaaag gaagagcccg caccgcctcc
     3001 ggccgccgcg cctgtggccg caccggctcc ggccaaagtg gagcctttga agattgcatt
     3061 cgcctatgtc ggcccggttg gcgacggtgg ctggactttt gcgcacgaca acggccgcaa
     3121 ggaaatcgaa aaggaatttg gcgacaaggt ggtcaccagc ttcgtcgaaa aagtccccga
     3181 gtcggccgat gctgaacgcg tgatccgcga catggccagc cagggcaaca agctgatctt
     3241 cggcaccacc ttcggctaca tggagcccat gctcaaggtg gccgccgact tcaaggacgt
     3301 gaagttcgaa cacgccaccg gctacaagac cgcggacaac ctgcgcacct acgacagccg
     3361 cacctacgaa ggtgcctaca tggccggcgt gatcgccggc aagatgagca agaccggcac
     3421 gttgggtgtg gtcggctcgg tgccgatccc cgaggtggtg cgcaacatca acagcttcac
     3481 gctgggtgcg cagtcggtca accccaaggt caagaccaag gtggtctggg tcaacgagtg
     3541 gttcaacccg ccgaaagaaa ccgaagccgc caccgcgctg atcaacggcg gcgcggacgt
     3601 gttgttccag aacaccgact cgtcggccgt gctgcagacc gccgagaaga tgaacaagcg
     3661 cgctttcggc tgggactcgg acatgaccgc ctacggtccc aaggcccacc tcggttcggc
     3721 cgtgatcaac tggggtcctt actacgtcaa ggccgtcaag gacgcgcttg aaggcaagtg
     3781 ggcgacaggc cagacctggt ggggcgtgaa agaaggcgcc atcgacatgg tgtcgattgc
     3841 cgccgacgtg cccgatgaca ccaagaaacg catcgacgag atcaaggccg gcctcaagga
     3901 tggcagcttc tcgatctgga aaggccccat catggacaac accggcaagg agctggtggc
     3961 caaggacgcc gtggccgacg acaaattcct gggcggcctg aagacctacg tcaagggcgt
     4021 ggaaggcaaa gtccctggca actgatgcct gataccgtgc ggtacggcgg cgccaggccg
     4081 ctgaccgtgc agtagcaaag cccaagccgc ctccgggcgg ctttttctgt ttttggagcc
     4141 gacatgaatt ccgtccgacc tgtatccccc gaccgcctgc ccgatctgct gcgcgccatg
     4201 cccaaggccg agttgcacat ccacatcgaa ggctcgctcg agcccgagct gatctttgcg
     4261 ctcgcccggc gcaacggcat ccagctgccg tacgcgagcg tggatgacct gcgccgcgcc
     4321 tatgctttta ccaacctgca gagtttcctg gacatctatt acgccggcgc cagtgtgctg
     4381 atcaccgagc aggatttcta cgacatgaca cgggcctacc tggagcgggc tgcgcaggac
     4441 aacgtgatcc acacggaaat attctttgac ccgcagaccc acacggcgcg cgggatcccc
     4501 atggaaaccg tgatcaaggg actgcaccgc gcctgtgtcg atgcgcgggc cgagctcggc
     4561 atcagcgccg cgctgatcct gtgtttcctg cgccatctca gcgaggaaga agccttcgag
     4621 acgctggagc aggccctgcc gttccgcgac ctcatcatcg gtgtggggct ggactccggc
     4681 gaggtcggca acccgcccga gaagtttgcg cgtgtgtttg cacgctgccg cgaactcggc
     4741 ctgcacctgg tggcgcatgc gggcgaggag ggcccgccgg aatacatctg gacggccctg
     4801 gacctgctca aggtcgagcg tattgaccat ggcgtgcagt cggccaaaga cccgtcgctg
     4861 atgcagcgtc tggccaaaga ccgcatcgcc ctgacggtgt gcccgctgtc caacctcaag
     4921 ctgtgtgtgt ttccggacct ggcccggcac aacctgcgcc agttgctcga tgccggactg
     4981 gccgcgaccg tcaactccga cgacccggct tatttcggcg gctacatgaa cgagaacttc
     5041 acgcagacct ttgcggccac cggcatggat gcgcagcatg cgtgtacgct ggcgagcaac
     5101 agtttcgaag ccagcttcgc cgccgcggcg gccaaacgtg gccacctgga gcggctggcc
     5161 gcctgtttcg agagttttcg ctagggcctg tcaacgctca ggacttggcg tcccggttga
     5221 cccaggggtc gggtgctgcg ccgggggagg ccggggcttc cggcagaccc accacggtcc
     5281 tgaccgcccg catcagcgcg tcggcctcga aaggtttggt gacataaccg tcggcgccac
     5341 cggccaggcc cttgatcacg gattcacgtg tggctttgcc ggtcagcatg atgaccggga
     5401 cgtttttcag ggccgggtgc tggcgcagcc ggagcaggat gtcgaaaccg tcggcgtcgg
     5461 gcagcatcac gtcgagaaga atcaggtcgg gaatcggggg cttgcggaat tcggccacca
     5521 cttctgcccg gttgcccgcc agccggacct ggaagccttc gaaggagaga taggtctgga
     5581 tgaatttggc gagcatgggc tcgtcgtcca ccacgatggc cgacagtatc tccccgggcg
     5641 ccagcttgcg ggctggtccg cggccgcgcg cgatgctgac gtagtagccg gccttcttga
     5701 gggacgcagc gcccgagtcg gcctcggctt cggccttgga cagggcgagc tggttgagct
     5761 ggatgttgaa ctgttcggta aaggggtcgc tctccgcatg cgccagcagt ccgcgaatca
     5821 gcaggctctg cagggttgct gcgagtgttt cgacggggac agcaggcatg cctgcactga
     5881 tttgcgcgaa gctcaggatg ccgtcgatgc ggatc
//
LOCUS       DAIDFG010000196         5904 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_196_length_5904_cov_18.203625, whole genome shotgun sequence.
ACCESSION   DAIDFG010000196 DAIDFG010000000
VERSION     DAIDFG010000196.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196_length_5904_cov_18.203625"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          932 a         1720 c         2007 g         1245 t
ORIGIN      
        1 gctgagcttg agcgccagcg aaggcgcaaa gctgtagcgg tccacaaact gctggtcgcg
       61 gtagctgccg gagtcttcgc gcgccgcgtt caggcggaag gcggcggctt cgcccagcgg
      121 caggttcacg tcggcttcca cgcgcctgag cttgttgctg cccaggatca gcgtcgtctc
      181 gcccgaggtt tcgccgaact tgggcttttt ggtgacgcgg ttgatcaggc cgcccgaaga
      241 gccccggccg tacagcacgg cggaagggcc cttgagcacc tcgacccgct cgacgttgga
      301 caggtcgcga aagtacaggg cgtcgtcgcg caggccgtcg gagaactggt cggcaatcgc
      361 cgtgaagccg cggatcatca cctggtcgcg ctggccgtcg ccatggctgg ctgccacgcc
      421 gggcacattg cgcagcgcat cttccatcga acgggcggcc tggtcgcgca gcaactggcc
      481 gggcaccacg ttgacggtct gcggtatgtc gcgcagcggc gcgctgcttt tggtggcggt
      541 gctgctgctg gacggggagt agccctgttc ggtgtcggcg cggatggtga tgtccggcag
      601 gacctgggcg ccgaccagct gcgcggcgca cagcgcgagc ggggtgaggc tgaattttgc
      661 tacaagtctg gtagctgatg gcgcccggca gacgggggct tcgaaggttt ttgatgcaag
      721 gaaacgggat gtaatggaca tgtgaagcac ggctcaggca ggttgctggc tggcttcaca
      781 ttccggggat atgtcggctg gcggtttaca aattttagct taataatgag aattattcgc
      841 gtttaagagt ctgttggtcc tgcctttgac ggcaaaaaac gacaggctcc ggtgtgagcg
      901 tcagaagtag aacgtggtgc tcagcgcgaa gctgcgcggt tcggccggcg acaccacgtt
      961 ggcgttgatg gcgcctgcgt aatacacctt gttgaacagg ttcttgatgc ccgccgtcac
     1021 gcgaaagcgg tcgccctggt aactcagcga ggtgtcggcc acaaagtagg aaggcagcgt
     1081 gaacggcagc gcgcccacct gctcgctgac gtaacgcccg cccaggccca cggtcacgcg
     1141 cttgaggtcc tgcaggcggt aagtggccca ggtggacagc gtgtgtttgg gcgtcaggtt
     1201 gatgggtttg ccgacgatgg cggcattgtt gtcgcgagtg acttccgcat cggtataggc
     1261 gtaggcggcg gtcagcttcc agctgttgcg caggtcggcg cccagctcca gttccaggcc
     1321 gcgcgcccgc tgctcgccgg tctgcacgct gaagtcggtg ttgaccgggt cggctgttgt
     1381 cacgttctgg cggcgcaggt catagatggc cagcgcacct gtgacttgcc ggttggcggc
     1441 ctcgtacttg aggccggctt cccattgctg gccggtctcc ggaacgaaag cgctgccatc
     1501 aaaggccagg ccggacaccg gaaggaaaga ttcgccataa ctggcgtagg tggaccagcc
     1561 cggcacaatt tcgtaaacca ggccgcccga gaaggtgttg gccttgtcct tttgcagcgt
     1621 gcgccggttg gtcagccggt tctcggtgtc ggtcgttgaa cggtcatggc gcaggcccag
     1681 cagcgccgtc cagccctggc cgaacttgat ctggtcctgc acatacagcc cggccgcagt
     1741 gagcctggat ggcgcgtcgc tcgtgaggtt ggcggggcag gtggcctgca cgccgtacac
     1801 cgggttgtac aggttcatgg gcgcaatcgt gcatctgcgg ctggcctgcg aactggtgcc
     1861 ggtgcgggcg tccagcccgg tgaccaggcg gtgcttcagg cccagggcct cgaagctggt
     1921 cagcagcgag gtgtcggtgg ccagcaggtc gtagtccatg tactggcggg tggcggtgcg
     1981 gttctgcagg cgccggttgg cctgcagcgt gccgttggcc acaaagttgc cggtgccttt
     2041 ttcttcttcg tagcgcacgt tctggcgcag caccacctcg gggttgaact cgtgctccag
     2101 cgaataaccc acggtggttt gctccatgtc gtaggccccg aagccggggt cgccggtgta
     2161 acgtgtcagg ggaatcgccc cgttggggtt gggcagcagc gtgccaaacg gggtgatgcc
     2221 ctgctggcgc atccactggc tgtggctgta ggtggcaaaa agcaccaggt cggtttgcgg
     2281 gccgaggtcc agtgccagcg agggcgccac ccagcggtcc ttgcggtaga cgaggtcggt
     2341 cgcatcgtcg gtgtcggaca cctgggcgtt gatgcgaaac gccgccttgc ccgaatcgga
     2401 cagcgggcgg ttcaggtcgg ccgacagtgt cttgaggccg aagcttccca gctccagcga
     2461 agcctcggac agcgcttcct ttttgggccg cttgctgacg gcgttgacga tgccgccggg
     2521 ctgcacctgg ccgtacagca ccgacgccgc gcccttgagc acctcgaagc gttcatagcc
     2581 ataaggtgtc aggcgtgtcc acatgccggg gctttgcacc aggccatcga tcaggatgga
     2641 ttcggtcgag cggaagccgc ggatgttgat gtcatcgaag ccgcgccggc cgaagttgac
     2701 cgggctgacg ccggcgactg tctgcagggc ttgctgcagg ttggtcgcct ggcgcgactc
     2761 catctgctcg cgcgtgacca cactgatgga ttgcggcgtc tccagcaggc gcatcggcgc
     2821 cttgccggcg ctttgtgcct ggttgggcgc gaaaccttcg tctttttcga cggtggcgtt
     2881 gatgcgcacc tcgcgcagct gcggcagctc gctgcccgta cccgaggcgg tggcctgagc
     2941 cggcggcgtt tcggcccggg ccagttgcgc catacacagc gccagcggcg tgaggacgaa
     3001 ggttgctaca aatttgctag ctgcttgcgc tcgctgcaca tgggctggaa gggtttttga
     3061 gtgagaaaaa gaggagggga gtgacatggt gaaacacagg ccggttgctg gctggcttca
     3121 catgaacatg tcggctggcg ggggctatag tttaacccgt aaatgagagt tgttctcatt
     3181 tgaaagaggc gctggccgcc attcgaatca ggcagggcct tgtcgaccat gaaacaaggc
     3241 agcgcttgcg gcgctgcgca gggtacagcc tagctcaatt cgcccatctg gctttgcagg
     3301 tagttctgga ggccgacctt ggccaccagc tcgatctggg tttccaggaa gtcgatgtgt
     3361 tcttcggtgt cgtccagaat gccctgtagc aggtcgcgcg agacgtagtc acgcacgctt
     3421 tcgcagtggg caatgccgtc cttgatcgtg gactgcgcgc cggtttcggc cttcaggtcg
     3481 cagttgagcg cttccggcac gtcctcgccg atcatcagct tgccgaggtc ctgcaggttg
     3541 ggcaggccgt cgagcatgaa gatgcggtcc atcagtttgt cggcgtgttt catctcgccg
     3601 atggactcct cgtattcctt cttcgcgagc ttgtccaggc cccagtgctt gtacatgcgg
     3661 tagtgcacga aatactggtt gatggcggtc agctcgttct tgagctgggc ctgcagatgg
     3721 gtgatgactt gtgcgtcgcc tttcatgccg aatccttttt ttggttttga gtattccgat
     3781 tgtcggcagg ccggtgtcat tgggcaagga gaaatgcgta acccttcgcg cgcgtcatgg
     3841 gcacgaatgg agtgatagcc gttcttcttt gctgcgggga gcgccagggg gccgctcccc
     3901 gcgctgtcag gccgccggtg ccgaggtgcg catcaggtgg tcgaaggcgc ccagggccgc
     3961 cttggcaccg tcaccggccg caatgatgat ctgcttgaag ggcaccgtcg tggcgtcccc
     4021 cgcggcaaac acgccgggca gcgaggtctg gcctttggca tcaacgatga tctcgccgtg
     4081 tttcgagagc tcgaccacgc ctttgagcca gtcggtgttg ggcaccaggc cgatctggat
     4141 gaacacgcct tcgagcgcca cggttttgag ctcgccggtc gcgcggtcct tgtagaccag
     4201 gccgcccacc ttctggtcgc cggtgatctc ggtggtctgc gcgttggtga acacgtcgac
     4261 gtttttcagg ctcaaaagct tgcgctgaag cacggcatcg gcgcgcaggg cggtgtcgaa
     4321 ctcgatcagc gtcacatggc cgacgatgcc ggccaggtcg attgccgctt ccacgccgga
     4381 gttgccgccg ccgatcaccg ccacgcgttt gcccttgaac agcggtccgt cacagtgcgg
     4441 gcagtaggcc acgcccttgt tcttgaactc ctgttcgccg ggcacgttga tgttgcgcca
     4501 gcgcgcgccg gtggagatga tgactgtttt gcttttgagc gaagcgccgc tttcgagctg
     4561 aacctcgatc aggtccttgc cgggtccgct attgggaata agcgccttgg cacgctgcag
     4621 gttcatgatg tccacgtcgt agttgcgcac gtgttcttcg agggcgtggg cgaacttggg
     4681 gccttcggtt tccttgatcg agatgaagtt ctcgatgccc agcgtgtcca gcacctggcc
     4741 gccgaaacgc tccgacgcca cgccggtgcg aatgcccttg cgcgccgcgt acacggccgc
     4801 cgccgcgccg gccggtccgc cgccgacgat cagcacatca aacggatcct tctcactgat
     4861 ttttttggct tcgcgctcgg tgccgctggt gtcgatcttg gcgaggattt cttccaggct
     4921 catgcggccg ttgccgaaca tggtgccgtt gaggaagacc gtgggcacgc ccatgatctc
     4981 gcgctccttg atctcgtcct ggaaggtgcc gccttcgatc atggtagtct tgatgcgcgg
     5041 gttctgcacc gccatcaggt tcagcgcctg caccacgtcc gggcagttgt ggcaggtcag
     5101 cgagacatac acctcgaagt cgaggtcgcg gtccagtgcg cggatctgtt ccagcacttc
     5161 ctgctcgacc ttgggcgggt agccgccgat ctgcaacagc gccaggatca gtgaggtgaa
     5221 ttcatgaccc atgggcaggc cggcaaaacg cgggccgtgg ttttcacccg ggcggttgat
     5281 cgagaaagag ggcttgcgat gagggtcgtc gcggctttcg gtcagcttga ccagtggcga
     5341 cgcctctgcg acgtccttca gcagcgacag cacttcgccc gaagcctcgc tgtcgtccag
     5401 tgaagcgacg atttcgatgg gcgcggtggc gcgttcgagg taacttttca gttgggcttt
     5461 ggtggcgtcg tcgagcatgg cggactcact ttcaatttca atggggaaca aaaaagcccg
     5521 gggcacacac cgaggtgtgt ttcgcccggg cttggactgc gagggcttag atcttgccga
     5581 cgaggtcgat ggacggggcg atggtcttgg cgccttcgtt ccacttggcc gggcagacct
     5641 ggccggggtg cgctgcggtg tactgggcgg ctttcagctt gcgcagggtt tccttgacgt
     5701 cgcgggcgat ttcgttggaa tgcacttcag cggtcttgat gatgccatcg gggttgatca
     5761 cgaaggtgcc gcgcagtgcg aggccggctt cttcgatcat cacttcgaaa ttgcgcgaca
     5821 gggtgccggt cgggtcgccc accagtgggt acttgacctt cttgatggca ggcgatgcat
     5881 cagcccagcc cttgtgcacg aaat
//
LOCUS       DAIDFG010000197         5824 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_197_length_5824_cov_18.088057, whole genome shotgun sequence.
ACCESSION   DAIDFG010000197 DAIDFG010000000
VERSION     DAIDFG010000197.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5824)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197_length_5824_cov_18.088057"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1090 a         1847 c         1861 g         1026 t
ORIGIN      
        1 cctcgaccga tccgttggag gctttgtagg tcttgtcgac accgctgact tgcagggcag
       61 gcaccttgaa acggtcagcc gatggaaggg attcgtcaag catggtgttg ggggctccag
      121 cgcatcaggc ggttgttgat gaggacaatc acgcgatcac ataaaaatcc ggcgatgccg
      181 ataaaaatca tgccggtgat gacgaggtcg gtgcgggaca gggttcgcgc atccatgatc
      241 acggcaccca gacccagctg cacacccgtc atttctccaa ccacgatcag gatccacgca
      301 aaggacaacc caagccgcag gccattgaag atgctgggca acgccccgcg caaggtcacc
      361 gaccggaact gggccgtgcc ggtgcagccc agcatggctg ccgcttcgaa cagccgggga
      421 tcgactgatc gcacaccgaa aacggtgttg agcatgatcg ggtagaaagc accgagaaac
      481 accaggaaaa tcgcggaatt ggcgcccagg ccgaagaaaa tcatcgccag cggaagccag
      541 gcggtgaccg gtatcggccg cagcagcgac agcatggggt ccagcatcat gcgcaggagc
      601 tgcgaacgcc ccagcatcag gcccaacggt attcctgcca aagcggcaag gccgaagccg
      661 aaaaacacgc gcccggtcga tgccgacaaa tgcttcagca gcgtggcgct gaaggcatcg
      721 tcatacacgc cgccgaacgt gaaatcccac atcatcaccg ccacggcttg tggcaaggga
      781 atcagcatcg tgccggacca tttgacccat gcccaccaca gcagcagcag caggacaggc
      841 accaccagcg ccagcgccgt ggcaccgcgc cagccggctg gtgaattttg tgccgtgacc
      901 ggcagcgccg gcgcgagggc cgcggcggcc gcaggctcgg ccatggtttg agcgctgaca
      961 gacatgatca ggaagacagc gcgctgagtg tcgccgtggg gatcagacgg ctgtaatccg
     1021 gcagcgccct gatctgcttg cgctccagca tttgcgagcc gtaatagcgg gcctgggtca
     1081 cgaacttgtc gtcgatgcgc caggtcagct ccacattcgg cgcagccaac tcgatcgagc
     1141 gccgctgctg ccccagacgt tgcatggcca tggcaatgaa ggcttccttg tccgacatgg
     1201 caaattcact ggcggcacga tggattttca gcaaggcgcg caccttctcc gggtctttct
     1261 tcagcatggc ttcgctgctg gtcagcacca tgttcaggga accggtcggg gtcgtgtagg
     1321 ggtactccag aatgcggccg gtgccgttgg ccaggctgat gccaggtgcg ggctctgcac
     1381 cgacataagc gtccagatcg ccgcgagcca gcgccgacgc catgtcgcta aaggaaaccc
     1441 gcacgacttc aacgtcgcgc agcgtcattt tctcggtggc gaggcggtcc atgaacacga
     1501 cctcctgggt ggaaccgggc cagatgccaa tgcgtttgcc cttgaggtcg gtgaacgtcg
     1561 tcaatttgga ggatgcggca gccacgatgg ccatgccgcg gttgcatgct gcgccgacga
     1621 tcaccacggg ttcgccggcg gcagcaccca gcgtcgccgc ggccaggccg tagatgccga
     1681 aatcgaccgt gccggtcacg accgcattct tgccatccgt cgggctttca aaagggatca
     1741 cctcgatggt ggtgccggcc ggcgcgaact ttgaatagaa atacggggta acgccatgaa
     1801 tcagcttgag cgtgccgacc ttgataacgc gctgctgcgc aaaaacaggc gacaggcgca
     1861 tgcctgtcag ggtgacggcg gcgagggccg ccgtgcgttt gagtaccgag cggcgggtgg
     1921 gatgggtcat ggtcaaactc cttggcgagt caaaaaaaca aacattgaag gacgacatct
     1981 ataagcgcca cgctatggag cctggcgccg ggtggcttct gctgcattgc agcattaagg
     2041 caatattagg ttgagaattt acaaaagtac attcagaagt aaaaatgcca tccataaatt
     2101 aaacttatac atttggagtc cggatgcaaa atcctctcga cctgcggctg ctcaagacct
     2161 tcatcctgct ggtcgaaacc ggcagcgtgt cagaaaccgc caggcgggtc agccggaccc
     2221 agcccgcggt gaccctgcag ctgcaaaaac ttgaagaact cgtgggcgca cggctgtaca
     2281 acaccgtcgg tcgcaaaccc gtcctgacgc cggagggcga actcctgctg ggctacagcc
     2341 gcaccattct tggcctgcac aacgaggtgc tgacacggct gcacgccccc cggctgggag
     2401 gccgggtcat ccttggcata ccggatctct atgccgccta cgtgctcccg cccgtgctgt
     2461 cggccttcaa tctggcgcac cccaatgtcg atgtcgaaat tcgctgctca ctgtcgaacc
     2521 gcctgatgac ggccatcggg cgcgacgaaa tcgatgtggc actggtcacg ggcatgccgg
     2581 ccttcaagca gggtctggtg gtggcgcagg agccgctcgt ctgggtggcc ggcgaaacag
     2641 gcccaccgca gcacgtcaac cccttgccgc tggcgatgct gccgcccgga aatatctttc
     2701 gcgaccatgc gctggcagcc ctcgatcagg tgggccggag ctggcgcctg gtttgcgtga
     2761 gcgaaagcct gagcgggatc caggcggcgg tactgggcgg catcgcggtg agtgtggtcg
     2821 gtctgagttc agtgataccg ggcatgcgcg tgctggggcg cagcgacagt ttcccggcgc
     2881 tgcccaaggt cgacctcacg ctgtaccgcg ccgcagggcg caacaacccg gcctcggatg
     2941 ccctggtcgc tgtcatcacc cgacacttcc ggcaatcggc catggcgccg acggaccgcg
     3001 ttgcgcccac gcaggcagga agtggcgcgc atgagtaagc acctgagaac ggcagggcat
     3061 ccgccctgcc accctgcaac acaccctgaa cacccgggtg tgcttgttgg ttaccatgtt
     3121 gctggtgctc cggcctgatg ccagggctcg cccatcaccc ccctggttgg gacgcctcac
     3181 tcaaaccatc tgaagaacaa agcccgtgat cactgccctc aaacttgttg ttctcctgct
     3241 tgctgctctg ctggtcatgc tgctggtggc cggtcagctg ggtttgttcg tggggaaacc
     3301 gccgcaagac ctgggcgtgc gggatggccg cctcaagccg ccgtcaccca caccgaacag
     3361 cgtgtccagc caggccgcca gccatgccga ccacccgcag cgcagtggcg ccgacatcgc
     3421 gcccttgcgc tacaccggtg acgccagggt cgccctgcgc cgcctgtcca ccttgctgga
     3481 atccacgccc ggttgcgtgc tggtgacgca ggagccggct tacctttacg cgcagtgccg
     3541 cacccggtgg ctgaaattca cggacgatgt ggagctctcc ctcgatgaag cgggcggcgt
     3601 gattcatgtg cgctcggcca gccggctggg gcgcaaggac tttggcgtga accgcgcgcg
     3661 cgtggaggcc ctgcgcgcac gttttgatca agccccaaga ccctagttag ctaccaaaac
     3721 aatagctggt tgcgcccgtc cctattgggc tggaggcact tttggcatgt aatcaggaac
     3781 tcagcccgct gccggccgac ggtggtgcgc aaaaaaggtc cacatcatca gcgtggcgtc
     3841 ggggccatcc ggcgcgctga aatccacact cgcatcgccg ccgctccagg cgtggcccag
     3901 gtgttctatc tcgcaactca ccaggtgcgg ctttctgccg gcgtaatacg tggcgctttc
     3961 ataggcatgg cgcggatggc gcccggtggt gcgcgccggg tagcggcgca gcaccggttc
     4021 cgggcgggtg atggccgcgg cgttgaaaat cgtgaactgt tcggtcaact gcgtcatgtt
     4081 gatgcgccgc accacggcat cgcgcgcgcc gtggatgaga atcgccggca tgcccgggaa
     4141 ggccggcagg gccgcggtga aggaccgcgc cgcatcgctg tgggctgcac cggcgccgtg
     4201 ctgcatggcc cggtaggcgc tcacgggcga atcggtcgtg ccgaacacgg gtgccgagtg
     4261 caggcccacg gcggccaccc ggtcggggta gcgcagggcc aggatgttgg ccagcccggc
     4321 gccggccgac aggcccgcca cataggtacg cgtcgggtcc agcgcgtgtt tggcctgcac
     4381 atgggccatc atttctgcaa tcagccgaac gtcgccctgc ccctcctggg tggcacgttg
     4441 gtaccagtgc cagcagcggt gcgcgtcgac cgtggccgac tggtgcgggt agagcacggc
     4501 aaaacccttg cgttcggcca gctggttcat gcgcgtggaa atggcaaagt cggtggcggt
     4561 ttgctggcag ccgtgcagca tgaccaccag cggccggggt gccgcgttgg ctgctgccgg
     4621 caggtagagc cagtacaaca tgcgccgtgc gggcgcgagc tggccggcgg ccggcacgga
     4681 gaaatacgcc ttttgccagg ttccgggcag gccggacacg gcgcgcgtcg gcggcgcagc
     4741 gggtttacgc agggctttca gtgccttggg cctggccagc ttgacgggct tggcgcggcg
     4801 caccgggctg gcggcaaccg tgggcaccag gctctggaac agccggcggc cctgcacctg
     4861 ctggaccctg cccatgcggc gcatgctttt cagccagagg ctgctcaggc ttttggccat
     4921 ggggctccgc agaggcttga attggttagc atgcattcat tttgctgcat tgcagcatag
     4981 acgtccacgc catcggctgc cgtttgtgtg gtccttcacg ccgcgtgtct gtcaggacac
     5041 tgacgtggcg caaggggtgc cgcctcagcc cttggtagga tgggaaaatt cgtgttgttt
     5101 gcgccttgtt tttgcacgcc tttttccccc tctctgcctg ccatgcctga tttcctgacc
     5161 gccgccctct ataaatttgt cgatcttccc gacttcgccg acctgcaggc gcccctgcag
     5221 gccttttgcg aagcccagga cgtgcgcggc accctgctgc tggcgcagga aggcatcaac
     5281 ggcacgattg ccggccccga aaccggcgtg cgcgccgtgc tggcgtatct gcatgctgat
     5341 ccgcgcctgg ccgacctgcc gcacaaggaa tcctgggcgc aaaagctgcc gttttaccgc
     5401 atgaaggtgc gcctgaagcg cgaaatcgtc accctcggcg tgcccggcat caacccgaac
     5461 gagatggccg gcgactacgt gaagccgcag gactggaacg ccctgatcgc cgaccccgac
     5521 gtggtggtgg tggacacccg taacgactac gagttcggca taggcacctt cgagcgcgcg
     5581 ctcaatccca aaacccggag cttttctgaa ctgcctgcgt gggtgcaagc gcagtccgca
     5641 ccgggcgggc tgctggccgc caaagacggc aaaaagccca aagtggcgat gttttgcacc
     5701 ggcggcatac gctgcgaaaa atccaccgcc ttcatgcgca cccagggttt tgacgaggtt
     5761 taccacctag agggcggcat cctcaagtac ctggaaaccg tgcctgaaac cgacagcctc
     5821 tgga
//
LOCUS       DAIDFG010000198         5820 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_198_length_5820_cov_16.797745, whole genome shotgun sequence.
ACCESSION   DAIDFG010000198 DAIDFG010000000
VERSION     DAIDFG010000198.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198_length_5820_cov_16.797745"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1176 a         1807 c         1760 g         1077 t
ORIGIN      
        1 agggggccgc tgcgcctgcg ggccggcaaa gccggacccg cggcccctgc ttgaaaggga
       61 aagagccccc actgtcgctc gccgtgctta gatccccttg cccccggggg ggggcttgtc
      121 ctggacggca tgtcgaaaca cccgcccatg aaaaaagccc ggcaacgcga gttgccgggc
      181 tttttggttg aagctgaccg aaggttccgg tcagttcagg actcagcgca cgggatcggc
      241 acgcagggtt gggtagcgca catgctcgac caactcctgg accttgcggc tgggcgccgg
      301 ggtgaccagg ctgaccagga tgatcacggc aaagccgata ggcacaccga agacaccggc
      361 cgagatcggc tggatttcaa accagagctg aaccggtgag gtgatgccga acaggccgcg
      421 catccagggc tgggtggtga tcatgtagaa gaacgtggtt cccaggccgg cgatcatgcc
      481 cagagaggca gcgataccag tggcacgttt ccagaagatc cccagcgtca gggccgggaa
      541 gaacgcagcc gcagcgaacg agaacgccgc ggaaaccagg aacagaatgt cggccggttt
      601 ctgggacgcc acgtaagcgg cgcacagcgc aaccaccagc agcaggacct tggagatggt
      661 cacgcggcgt gcagtcgatg cgttcgggtc gatcattttg tagtacaggt catggctcag
      721 cgcgttggcg atcgtcagca gcaggccgtc agcagtggac agcgccgcag ccagaccacc
      781 ggcagccacc aggccggaca ccacgtaagg caggccgcca atcgcgggcg cggccagcac
      841 gatgatgtca ccaccgatgg acatttcgtt gagctgcaaa atgccgtcct tgttgatgtc
      901 caccaccgac agcagcgaag gatcaacctt gttccaggcg gaaatccagt ccggcaacgc
      961 gttgaacggc gtgccaatca gcaccgtgaa tacctcgtat ttcaccagca cagccagggc
     1021 cggcgcggtg aagtacagca ggaaaatgaa gaacagcgac caggtgacgg attgccgcgc
     1081 ttccttcacg ctgggcaccg tgtagtaacg catcaggatg tgcggcagcg cggcggtacc
     1141 caccatcagg cagaaaatca gtgccaggaa gttgcgacgc gaaacgtcgt aagcatgctg
     1201 agccttggca tcgccattgg ggtcaccggc aaactgctgg gcgtgcggtg gcatgccatt
     1261 gagcggcaag gccttggtgt cagctccagc cttggcagcg gcccacgcct tcttggctgc
     1321 agcttcatct ttcggcaagg cagcgagcgc tttttcagcc gcggaaattt ccgacagcgc
     1381 gccgttggaa gctttcaggt ccgccacggc tttctccgcg gcggccttgt cggcagccag
     1441 cgcagcggcc gggttcttga gcttttcagc cagggctgcc gaacgctgct tgaagatatc
     1501 gcgaacttcg atttccttgg cgttgctggt cagctctttt tccttggcag tcactttttg
     1561 cagctggaag ccgtaaattg cctgcggaat cgggacgctg gtctgtttga ccgacagcca
     1621 caccaccggg atcatgtaag ccacgatcaa aatgatgtac tgggccacct gcgtccaggt
     1681 cacagcacgc atgccgccca ggaaggagca gaccagaata ccgcccagac ccaggaaaat
     1741 cccgagttca aaggccacac cggtcaggcg cgcggtgatg atgccgacgc cgtaaatctg
     1801 cgccaccaca taggtgaagg agcacagaat cgcagcgacg atacccagaa aacgtggcag
     1861 gttgccgcca taacgctcgc ccaggaagtc ggggatcgtg aactggccga acttgcgcag
     1921 gtagggcgca agaaacaggg ccaccaggca gtagccgccc gtccagccca tgatgaaggc
     1981 caggccgctg taacccgtca ggtagagtgt tccggccata ccgatgaagg acgccgcaga
     2041 catccagtcg gcgccggtcg ccatgccgtt gtacatggcc gggactcggc gccctgccac
     2101 gtagtattcg gcagcgtcgg tcgtacggct catgatgccg atgccggcat acagaccgat
     2161 ggtggccagc aggaagatca agccgatcca ctggcgggac aagcccatct gctcaaggac
     2221 ggccagtacg accacgaagg ccaggaagcc cccggtgtac cacttgtaga ctttgctcaa
     2281 gcctgccttg aaggcagcat tggaggccgc ggtgttaccg ccggcgtcag aaccaaacat
     2341 tccagccatg attattcctc gctttcttca acaccgtgtt cgatgtcgag cttgcccatg
     2401 taccaggcgt aatagccgat gatcgccacg taaatcacca aagccccctg tgctcccatc
     2461 cagaagctaa aaggccagcc aaagaaagtg aagctcaact cgcgagcgaa atagctcacg
     2521 acgaacgtca cgacgaacca gatcacgagc aggattgccg tgacattcag gttcttgcgc
     2581 caataacggc gatggttgtc agtcaactgc ataacacttg cctccatcta acaacaccct
     2641 ttcgggcaca tcacgatcgc cacttgtccc caagcgtggc ctgcaagccg gatcgcgtct
     2701 cttataagcc tgggcccaga ccccggccgt caaataacca ctcagcgggg ctgccttcag
     2761 gaagactgag caatgctcag ccccgtctcg cgttccagct gggcggccac tttaggctca
     2821 aaccgtttga ggtggcgaat gactttcacc gcctcatcag ggtcttgcct gtcgacatga
     2881 acccgcgcaa gctgatacca gccgaacggg ctcatgggtt gcagttctgt atttttcttg
     2941 agtgcctgaa ccgcctcatc gaagcggcga tcacgaatca gcagcagagc caggccgtac
     3001 caggcccggt ccatcttggg ttcgatgtcc agcgcccggc gaaaggcggg ctccgcgtcg
     3061 gcgtggccac cgatgtcttc cagcatgtag gccaggttga accaggccgc ggcattgtcg
     3121 gggtaacgct ccgccagcga acgcagcgcg gcgatggcgc cgtgtttgtc gccttcctgc
     3181 gcgagaacgt gggcgcggct tgccagggca taacggtcac ccggtcgcag caccagcatc
     3241 tggtcgaatt tctccagggc cgcctgctgc ttgccaaaca ccagcaggac cagcgcctgc
     3301 aacttgagat accagtaggc caggatcgtg ttcatttgca aaactccaca ccaatttcaa
     3361 gacaaatccg gattttcatc agggtcgatg cgatccagac aaacagcagc aacaaaaacg
     3421 ccacgagcgg aacgtggcgg tcaatgattt gtttcggggt gatgaagcgg gcggccacca
     3481 caaagggttt gccgacgatt tgcaggatct ggtagaacag gttgctgtcc cttttagcgc
     3541 ctgccagcaa gccaagcacc cactgcccga acagcgcgag caatgcgatc tctgctatca
     3601 atttaagagt ggacacggca aaaagcataa gaccccaggc aacaatggct tgattgttgc
     3661 cccgggtctt accgacttct tacaaattgc cgcacagggg ggtgaacccc gttgaaaccc
     3721 tccctaggac tttcccttgg ttctggccgc ttgcgggcgc agctagaacc aagtacctac
     3781 aagggaaacc gtgcctgact tgcgccgtgg atctgcggcg caagtcagcc cggctggcta
     3841 gttggccaat tgcttccagg tgtcgatgac ggtgtcggga ttgagcgaga tcgacccaat
     3901 gccctgctcc ttgagccaaa gtgcaaagtc cggatggtcg ctggggccct ggccgcagat
     3961 gccgacgtat ttgccctggc gcttgcaggc cgcaatcgcc tggctcagca gggccttgac
     4021 ggccggatca cgctcgtcga agtccttggc caggatgtcc aggcccgagt cgcggtccag
     4081 gcccagggtg agctgggtca ggtcgttcga gccaatcgag aagccgtcga agtactcgag
     4141 gaactggtcg gccagcaccg cattgctcgg cacttcgcac atcatgatga tgcgcaggcc
     4201 atcctcgcca cgcttgaggc cgtgttgggc cagcagttcc gtgaccgcct tggcctggcc
     4261 cagcgtacgc acaaaaggca ccatcacctc gacattggtc aggcccatgt ccttgcgcac
     4321 gcgcttgagt gcttcgcact ccatggcaaa ggcctcggca aaatcaggac tgaggtaacg
     4381 cgccgcaccg cggaaaccga gcatcgggtt ttcctcttcg ggctcgtagc gcgaaccgcc
     4441 gatcagcttg cggtattcgt tggacttgaa gtccgacagg cgaacaatca ccggcttggg
     4501 ccagaaggca gcggcaatcg tcgcaatgcc ttcagccacc ttgtcgacat aaaaagcacg
     4561 cggcgaggca tggccgcgcg ccacgctttc cacggccttc ttcaggtcgg catcgacctg
     4621 cgggtagtcg aggatggctt tcgggtgcac accgatgttg ttgttgatga caaactccag
     4681 ccgggccagg cccacgccgt ggttgggcac ctggcagaag tcaaacgcca actgcgggtt
     4741 gcccacgttc atcatgatct tgatgtcgat cggcggcatc tcgccgcgct gcacctcggt
     4801 cacttcggtc tccagcaggc cgtcgtaaat gttgccggtg tcgccttcgg cgcaactcac
     4861 cgtgaccagc atgccgtcct tgagctgttc ggtggcgtcg ccgcaaccga ccacggcggg
     4921 aatgcccagc tcgcgggcaa tgatggcggc gtggcaggtg cgcccgccgc ggttggtcac
     4981 gatggcgctg gcgcgtttca tgaccggctc ccagttcggg tcggtcatgt cggtcaccag
     5041 cacgtcgccg ggctggacct ggtccatctc gctgatgttg tgcacgatgc ggaccgggcc
     5101 ggtgccgatt ttctggccga tcgccctgcc ctcggccagc acggtgccgg tgcccttgag
     5161 cttgtagcgc tgctcggcct tgcccttgga ctggcttttc accgtctcgg gtcgcgcctg
     5221 caggatgtag agctggccgt cagtgccgtc cttgccccac tcgatgtcca tggggcggcc
     5281 gtaatggtcc tcgatgatca ccgcgtaacg cgccagctgc tcgacgtcgg cgtcggtcag
     5341 cgagtagcgg ttgcgcagtt cggccggcac gtcggtggtc ttgaccagtt tgccgccggc
     5401 cttcttttcc tcggcggtgg tgaactccat ctgcagcagc ttgctgccca ggttgcggcg
     5461 gatcacggcg cgattgcccg ccttgagcat gggcttgtgc acgtagaact cgtccgggtt
     5521 cacggcgccc tgcaccaccg tctcacccag gccgtagctg gaagtgatga acaccacgtc
     5581 ttcgaagccg ctttcggtgt cgatggtgaa catcacgccg gccgcgccga tgtcgctgcg
     5641 caccatacgc tgcacgccgg ccgacagcgc cacgtcggca tgggcaaaac cctggtgcac
     5701 gcggtagctg atggcgcggt cgttgtaaag cgaggcaaac acctctttca tcttgtccaa
     5761 cacgtcctcg atgcccacca cgttcagaaa ggtttcctgc tggccggcaa acgaggcgtc
//
LOCUS       DAIDFG010000199         5757 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_199_length_5757_cov_16.569625, whole genome shotgun sequence.
ACCESSION   DAIDFG010000199 DAIDFG010000000
VERSION     DAIDFG010000199.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199_length_5757_cov_16.569625"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1041 a         1945 c         1809 g          962 t
ORIGIN      
        1 caggcgtagg cgcgggcccc catcacgttc ggggcgcgca agctcagcac gtggaagtgg
       61 ggcggcacgt ggggcgccag gccaaacagg tcgtcctcat tgctgccgac gccgtgcatc
      121 agcaccagca accagggctg ggctgcgccc gccgctgcgg gacggtgcag gaaagaaagg
      181 ggcaaatcca tcgtcatcag ggaactccgg gttgggcaaa cagggccagt tgatttcagt
      241 gcgccggcac gggcgcggca agggcgaagc tgtccatcgc caacatgctg tacatcacct
      301 cacggctcag gtagtcgggc cgcgcaccct gggggccacc ccaaccggcc gcacccagcg
      361 cgaagaagat cggcaggaag tggtcttcgc tggggtgcgc gcgctgcgcg tcggatgcct
      421 ggcgccggta gtccagcatg gcagaaaaat caccgcgttc cagcgtggac tcgacccagc
      481 ggctgaatgc aacgacataa ggcgccggct cgcgtgcacc gccaaaaaat tcgcccaggt
      541 tgtgcgtcat gctgcctgat ccgaccacca gcacgccgcg ctcgcgcagg ccggccaggg
      601 cctggcccag ggcataggtt tcggccgggc cataagacac cggcaacgcc acttgcacca
      661 ccggcacgtc ggcgtcagga aacaaatgca tcaggggcac ccaggcgccg tggtccagcg
      721 gccgcttgtc gtcgccctgc gcggcgatgc ccgccccctg caacaagccc agcacctcac
      781 gcgccagttc cggatggccg ggggcgggat attgcaaggc atacagatcc tgcggaaaac
      841 cgccaaagtc gtgccaggtg gccggcgcgg gattcgtcat caccgcgggc gtgcgcgcca
      901 tccagtgcgg cgacatgagc acgatgccgc gcaggccagt cccggccgcc tctttcagcg
      961 cccagcccca gcgcgtcagc gccggcccgg tcgtccccgg ctcggtggca aacagcggcg
     1021 ccccgtgcga gatgaacagc gcaggaagga cagagacagc agaagcggaa cgggcagaca
     1081 tggcgggact ccttgagaac aggaaagaac ccttccaata tagttgtcta ccctttaaag
     1141 ataaatatcg aatttattga ttaattgtct tggaatgcaa gacaatccag ctttcccaca
     1201 cctctcttct caattcctcg agcccaccat ggaccagttt cgccagatgg aagcctttgt
     1261 ggccgtcgtg cagagcggca gctttgtcaa ggcgggcgag cggctgggca gctccaaggc
     1321 catggtgtcg cgcctggtgc tggaactgga ggcgcgcctc ggcgtgcgcc tgctcaaccg
     1381 caccacacgc cgccagtcgc tgaccgaagc cggtgtggcg tatgccgagc gcagcctcaa
     1441 gatcctcgac gacctggccg aggccaacgc cgccgccagc gccaacaccg tgcaaccggt
     1501 cggccgcctg cgcatcaatg ccccggtgac cttcggcaac ctgcacctgg cgccgttgtg
     1561 gggccgcttc ctgcagcagt acccgcaggt cagcctggac gtgaccctgt ccgaccgcgt
     1621 ggtggacctg gtcgatgaag ggtatgacct cgccatccgc attgcacgcc tgcccgactc
     1681 cagcctggtc gcgcagcgcc tggcaagcac gcggctggtg ctgtgcgccg cccccggcta
     1741 cctgaagcgg caccccgcca tccgtacgct ggaagacatc gccgcgcaca cggtgattgc
     1801 ctacacctac tggtcgggcg gcgacacctg gtcgtttcaa ggaccgaacg gcaaggagga
     1861 cgtgctcacc caaccacgcc tgcgcaccaa ctccggcgac acctgcctgg ccgccgcgct
     1921 ggccggccag ggcctgatcc tgcagcccac tttcatcgtc gggccggccc tgcatgacgg
     1981 tcgcctgaaa gaagtgctgc cccgcttcca ggcacccacg ctggacattc aggcggtgta
     2041 cccgagccgg caacacctgt cgctcaaggt caggcgcatg gtggattttc ttgcagacgc
     2101 gtttgaaaaa cccgcctggg cggcggcgga cgtggcgccg acgggtcgct aatttgctat
     2161 taaatcagga gctgccggca cccattgaac aaggactaaa ggccgttttc ctgaaatttc
     2221 cggaggtggg tcgatactgg ttttttcgcc gctgcgctcc ttcatttgga ggatgcattc
     2281 cggtcggcac gcgatcagaa ttgcgcaata aatgggactg gtccctgcaa aggagcttcc
     2341 atgcgccatc tgatcattct ttcggtcgcc gtgttgtgca gcagctgcgc gcaacttcct
     2401 cctgcggcgg tggcggatgc gccgcccagg caggcgcagg ccgtggtgtt cgacatcgac
     2461 ggaacgctca cacccgccgt gtcgaccatt tttgaagcgc gcgaggatgc ggccagggct
     2521 gtgcgcctgt tcgccgacaa gggctacacc atcatctacc tcagcacccg tgtcagctgg
     2581 ttttcggcca acatccccgg ctggctgaaa gacaacggtt ttcctgccgg cagcctccat
     2641 gtggcacaaa cggatgaaga tcgcagccgc cccgatgtct acaagacgcg tatgttgaaa
     2701 gacttcatgg cccatggctg gcagctcagg tttgcctatg gcgactccga caccgatttt
     2761 ttggcctatg ccgcggctgg catcccgaag gaaagtgtct tcgcgctgct gcgcaggtac
     2821 gaggccgatt gccaaccggg tgcgtggaag gtttgcctga gggggtggac tgaacacctg
     2881 gacttcgtga cgcgatccgt gccgtctgcg ctgaccaact gacggcctcc tttgccacgt
     2941 cggtccggcg caaccgctat cgattcaata gctgatagcg cccgtgacac aagggccaga
     3001 gcccaaaaag gcttgaagct tcaagccagc tactcgctga ccccgccctt gccgcgcgag
     3061 gccttgatct ccgaacgctg gctttttcct gccaggcgcc gctgcctgga gccataggtc
     3121 ggcttggtgg cgcggcgcac gcgcggcggc acggcgacgc tgttgacaat ttcgttgagc
     3181 cgggccagcg cgtcggcgcg gttcatctcc tggctgcggt gttgctgggc cttgagcacc
     3241 agcacacctt cctgcgtgat gcggctgtcg cgcaggcaaa gcagccgctc cttcacatcc
     3301 tcgggcaacg aagaggccat gatgtcgaag cgcaggtgca ccgcgctgga caccttgttg
     3361 acgttttgcc ccccggcgcc ctgcgcacgg atggcgctga tctcgacttc ggcttcattg
     3421 acttccaggg gagcgacagg cggcatggcg aggcggcttg actcaggaac gctgcgcgtc
     3481 aaaaaaactc ggcaacagcc gggcgaacac cgcattcagg tgcgcggtgt ccatgcactc
     3541 gcgtggaagg gagttgtcgg ggggaggcat ggggcattct agaagacgct gccattggat
     3601 gaatcaagtc tgtagcttct tcgttccgtc tctgagcacc agcaagcccg caagcccata
     3661 aggtcagaaa agagaacaag agccaagaca cacctgagat ccccctgcac acccgccccg
     3721 aatcccggac aatcccctga tccgaccggc ttttcatgca cacccgacac ttcctccagc
     3781 tcatcgccct gtccgccctc tggggtggct ccttcccttt gcttcgcatt gccagcccgg
     3841 cgttcggccc gtggttgctg gccggtgtgc gctgcatcct ggctgcgctg gtgctgaccc
     3901 tgctgatgcg cgcgctgcgc gcgcactggc cgccgcgtgc ggcctggccg cgcctggcgc
     3961 tgctcagtgt gctgacggtg gtcgcgccct ttgtgctgtt caactgggcc gcgctggtga
     4021 tcccggccgg gtattcggcc gtgctcaacg ccacggcgcc actgttcggc gtgctgggcg
     4081 ccgcgctggc gcgcgaggag cggctcacgt cgcgccggct gctgggctgt gccgtgggtt
     4141 ttgctggtgt cgccctgctg gtgcagctgg ggccggtggc ggtgacaccc cgcgtggtgc
     4201 tggccgcgct ggcctgcacc gccgctgccg ccgcctacgg ctttggcgcc atcctgatga
     4261 aacgcgcaac catggcgcac gacccgctgc cggcctcggc cgtggtgcat gtggcggccg
     4321 cggttttcct gctgctgccc tccgctgcgg ccctgccctc ggcccacacc accagcggcg
     4381 cactgatcgc cgtggcggtg ctgggcatct tcacctcggg cctgatgtac tggatcagca
     4441 tgcgcctgat gcgcgagatc acggccagcg ccgccacctc ggcagcgttc atgattccgc
     4501 tgttcggcgt cgcctggggc ggcatgttcc tcggcgagcc ggtcacctgg ggcatggcgc
     4561 cgggctgcgt gctggtgctg gccgccacgg cgctgatcac cggcttcaac cccttccgcg
     4621 ccccgccgga acgctgaacg gttcaacgcc gtacgctact gtattgatag ctgcttgcgc
     4681 ccgcgggaca ggggctagag gcttgaaacg cttgtagacc tgccgcttgt cagggtgcgg
     4741 cagcttccag gctgcgctgc acgcgccagg cccggtaggc acaggccgtc gccaccaggc
     4801 tggcggccag gcaggcccag tacacactga ccagcccgaa agccgaactc agcagcccgc
     4861 cgaccagccc ccccagcaca cccggcaagc cgtaagccac caccacgtac agcgcttggc
     4921 cgcggccgcg cagccgtccg gggaaatggt gcgacaacag cgcgatgcag accgtgtggt
     4981 gggtggcaaa cgtcagcgca tgcaacgcct gcgcaaacag catgagcagc agcacaccgg
     5041 gccagacggc ggtgatgccc atgcgcagcg ccatggcagc cgaacacacc atcagccagg
     5101 cggtcaggct caaccgcggc agccagcggc cctgggtgta gaaccagccg atttccacca
     5161 ccaccgaaaa cgcccacaac acgccgatca tggttttgct gtagccattc gcgtcgaggt
     5221 agagcgagaa aaacacgtag atgctgatgt gcgccagcac atgaaaaaat gccgacgcaa
     5281 aaaaccacac cacggccctc tggcgaaaaa tcggccatac aggcagcttc agatcgtggg
     5341 cactgaccgc ctccttcagg tccggcagca gccagacgct gaccgtcacg gccagcagcg
     5401 acaggaaggt ccaggccgga aagtgtttca tgccaaagat ctcgaaccag ctgcccgcgg
     5461 caaacaccgt gaacaaaaag cctagcgaac cccacaggcg cacgcgtccg taacgccgtg
     5521 cgtcaaaggc cccgccctgg ctgatcaggt gcgccatggc ggcttcgctc atgggcatca
     5581 tggaactcgt gtgcgtgaac atcaccagca acacggccgt gagccaccag acgccgtaag
     5641 tggattcaaa gtccagccac aggccgaggg acgagatcag cgccaccgtg gcgccgtaac
     5701 gcagcagctt gacgcgctcc ccggtgtggt cactcagcgc gccccagccg tagggcg
//
LOCUS       DAIDFG010000200         5717 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_200_length_5717_cov_16.581067, whole genome shotgun sequence.
ACCESSION   DAIDFG010000200 DAIDFG010000000
VERSION     DAIDFG010000200.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200_length_5717_cov_16.581067"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          974 a         1817 c         1844 g         1082 t
ORIGIN      
        1 gctgacctct tgcatgccat gcaagcgctc tcccagctga gctatacccc cctgttgcaa
       61 gtttccctgc aatcgtcgag ccttgaatta tagcccgaaa atcagagcct tttcaagtgg
      121 acgagacgcg cgatcacgtt ttccctgtcc atcaatgaca aaaaagcatc gagcgacggc
      181 gtttgctggc gcccggcaac caacacccgt accggcatgg ccagttgcgg catcttcagg
      241 cccttgtcca gcagcacctg cttgatggcg gccgcgatcg ccggcttgtt ccactcgcag
      301 ctggccagca actccgcgag ctggtacacg gccggggcca catcggccgg cacatgggcc
      361 tggatgtctt cagcactgac cggctcatgg ctgaccagca cgcccatcca gtcggccagt
      421 tccaccaggg tgctgcaacg gtctttcagc agggcgcagg cggccacgcg ccggtctgca
      481 tcgaggccgg ccagcgcacg ctcattgagg aagggcaaca ccagttcggc aagcctgtcg
      541 tcggccagcg ccttgatgtg ctgggcgttg acccagcgca gcttggtttc gtcgaactgc
      601 gccgcacttt tgccgaggtg gtcgaggttg aaccattgca ggaactgttc gcggctgaag
      661 atttcatcgt cgccgtggct ccagcccagg cgcgccaggt agttgaccat ggcatcaggc
      721 aggtagcctt cttcggcgta ctgcgtgacc gccttggcac cgtggcgctt gctcattttt
      781 tcgccctgct cattgagcac ggtcggcagg tgggcgtaaa ccggcacatc cttgcccaag
      841 gcacggaaga tgttgatctg gcgcggcgtg ttgttgatgt ggtcgtcgcc gcggatcaca
      901 tgggtgatcg ccatgtcgag atcgtcaacg accacgcaga agttgtaggt cggcgtgccg
      961 tcgggccgcg cgatcacgag gtcgtcgagc tcgtcgttgc tgatctcgat gcggcccttg
     1021 accttgtcgt cccagaccac ggcgccgtgc tgcgggttct tgaaacgcag caccggcagc
     1081 acgcctgcgg gaaccggtgg cagggtcttg ccgggttccg gccgccaggt gccgtcgtaa
     1141 cggggtttct gtttttcggc catctgcttc tcgcgcaggg catcaagttc ggccatgctc
     1201 atgtagcagg ggtagacatg gcctgcggcc tgcaactcgg ccagcaccgc cttgtagcgg
     1261 tccatgcgct gcatctggta gtacggacct tcgtcgtagt ccaggcccag ccagcgcatg
     1321 ctttcgatga tcacgtccac cgcggcctgc gaggaacgct cgacatccgt gtcttcaatg
     1381 cgcaggatga agtcaccgcc ggtggcgcgt gcaaaagccc aggggtagag cgccgagcgg
     1441 atgttgccga gatggatgaa gcctgtcggt gatggtgcaa accgggttct tgtttttggt
     1501 gtcatgtcag ggtgcccaag ccgcgcagca ggtcggcctt gatgtcttca atatgctcca
     1561 gccccacggc cacgcggatc aggccctgcc cgatgccggc ggcctgccgc tgcacttcgg
     1621 tcaggcgccc gtgcgaggtg gtgccggggt gggtgatgat ggtcttggtg tcgcccaggt
     1681 tggtggcgat gctcagcacg cgggtgctgt tgatgacgtg gaaagcattg gcgcgtgcgg
     1741 tgtcggggtc gctgcccaca acgtcgaacg acaccacggc accgccctgc cccgactgct
     1801 ggcgcatggc cagttcgtgc tgcgggtggg aggccaggcc cgggtaataa acgcgcgcca
     1861 cgccgggctg cgcttccagc cagcgggcca cggccagcgc attggcgcac tgcgcctgca
     1921 tgcggatgcc cagggtttcg agtcccttga gcacaaccca ggcattgaag ggcgacagcg
     1981 tcatgccggc ggtgcgcacg accgggccaa acacatcgac gatcagcttc gacgggccgc
     2041 agatcgcccc ggccatcacg cggccctggc catcgaggta cttggtgccg gagtggatca
     2101 ccaggtcagc acccagttca gccggccgct gcagggccgg cgtgcagaaa cagttgtcga
     2161 ccgccagcag cgcaccggcg ccgtgcgcga gatcggccag ggcggcaatg tcgcagacgt
     2221 cggtcagcgg gttggtcggc gtttcggcga acagcagctt ggtgttgggc ttgatcgccg
     2281 ctttccactc ggctacatcg gtctgcgaga caaaggtggt ctcgacgccg aactttccga
     2341 attcacggcc gatcagtgtc agcgtcgagc cgaacatcga gcgcgaacac acgacatggt
     2401 cgccggcctt gagcaggccc atgcacatca tgaggatcgc acccatgccg gtcgaggcgc
     2461 ctatggcggc ttcggtgccc tccagtgcag ccagccggcg ctcgaagctg ctgacggtgg
     2521 ggttggacgt gcggccgtag gtgtagccgt cctcggtgcc ggcaaaacgc cgggccgcgg
     2581 tttcggcatc gggctgcacg aaaccgctgg tcaggtagag cgcttcggag tgttcgccgt
     2641 attggctgga ttccagcgcc acgcgcacgg ccagcgtgtc gcggtgcagg ttgtcgggca
     2701 gttttctgtc ggccatgctc aggcttcctg cgggttgggc agcgccagac gcgagacgtc
     2761 gacatcgccg tcttcgctgc tgtcaacgcg ctgctcgttc attttggcaa tgctctcggg
     2821 cgtgatgtcg ccggtgatgt agacgccgtc gaaacacgag gcgtcaaagc cgtcgagctt
     2881 gccgttgagc gaaccgatgg cgcgtttcat gccttcgaca tcctggtaga tcagcgcatc
     2941 acagccgatg gcgacgcgga tttcctcgac cgtgcggtca tgcgccacca gctcggctgc
     3001 ggtcggcatg tcgatgccgt agacgttggg gtaacgcacc ggcggcgcgg cgctggccag
     3061 gtaaaccttc ttggcgccgg catcacgcgc catctgcacg atctcgcggc tggtggtgcc
     3121 gcgcacgatg gagtcatcga ccagcaacac gttgcggccc ttgaactcgc tggcgatcac
     3181 attgagcttt tgccgcacgg attttttgcg cacgccctgg cccggcatga tgaaggtacg
     3241 gcccacataa cggttcttca cgaaaccctc acggtaggga atgcccaaca ggtgcgcgag
     3301 ctgcgttgcg ctcgggcggc tggactcggg gataggaatg atcacgtcga tttcgttggg
     3361 tggcacggtg gacaccacgc gtttggccag cgtctcgccc aggttcaggc gcgcctggta
     3421 gaccgagatg ccgtcaagca ccgagtcggg ccgcgccagg tagacaaatt caaaaatgca
     3481 gggcatgagc tgcggatggg ccgcgcattg ctcggcgtgg accgtgcctt ccatgtccac
     3541 aaacaccgcc tcgcccggcg cgatgtcgcg ctcgaactgg tgggccgtgc cttccagggc
     3601 caccgactcg ctggccacca tgacggtgtt gccgctgtcg ttctggccgc ggccgataca
     3661 cagcggacga atgccgaagg ggtcgcgaaa agccagcagg ccgtggccgg caatcagcgc
     3721 caccacggca tacgaaccct tgatgcgttt gtgcacgccg cgcacggccg caaacacgtc
     3781 agcgggcgtg agcggcaggc cgcgcgtggt tttttccagc tcgtgcgcca gtacgttgag
     3841 cagcacttcg gagtcgctgt cggtgttgat gtggcggtgg tcggtgttga acagttccgc
     3901 cttgagttcg gcagcgttgg tcaggttgcc gttgtgcgac agcaccaggc caaacggcgc
     3961 gttgacgtaa aacggctgcg cctcgtcttc gctgaaggcg ttgccggccg ttgggtaacg
     4021 cacctggccc aggcccacgt tgcccggcag cgcgcgcatg ttgcgcgtgc gaaagacgtc
     4081 gcgcaccatg cccttggcct tgtgcatgaa gaacttgcgt ccttgctgcg tgacaatccc
     4141 ggccgcatcc tggccacggt gctgcagcag aagcagcccg tcgtaaatca gctggttcac
     4201 cggtgcgtta ctgacaacgc caactattcc gcacatacat ttccgttcat caattcgcca
     4261 atcattagag gtactttgca aactgttcgg gcaacaccgg cttcaggccc ttgaccgccg
     4321 cggtcagcac gccggcaccg acggactcct gccaccaggc gctggccttg aacgcggtca
     4381 tgttgacgac caccgtcacc gccagcagca ggatcacccc gcgcaccagg ccaaaagccg
     4441 cccccagggt gcggtccacc ggccgcaggc cgatggcctc caccagcttc ttgaacaaca
     4501 ccgccagcag gcccgccaga aacacggaca cgatgaaaat cagtacaaag gccgcggcgt
     4561 agcgcaccgg atcggacgca ctctgcagcg gcagtacggc agccaccgcc ggcgcaaacc
     4621 attgcgcgat gtaaaacgac agcgcccagc cgaggacgga caggatttcg tacaccagcc
     4681 cccgccacgc gccgatgatc agcgacacca ccaacacccc cagaaacgcc cagtccgcaa
     4741 cagccacgct acaaggtcag gatggcggcc ggcaaatcaa ggcctttgat cttgctggcc
     4801 gccttgtcgg catcggcctt gctggcaaaa ggcccgaccc gcacgcgaat gcgcttgccg
     4861 tccttggtct cggccacctg ggtgtaggtc ttgagccccg cccgttccag tttctggcgc
     4921 acttcccggg ccttgtcggc gtcggcgaaa gcgccgacct gcaccaccag gcggccttcc
     4981 gcctcggccg gcgcggcctt ggcggcgggc gcggcggcag gcaccttgcc ttcgagcagg
     5041 gccttggcac gggcactctc gtcggcggca ggtggtgttg cggacgtttt ggccgcgacc
     5101 ttgggctctg gtttgggttc tggcttcggt tcgggttttg gctctagttt tgcttctttt
     5161 ttgatagcag tctgcgcaga ttcagcgggc gcctcggcct ttttttccgt gatgatttct
     5221 tcgcggggcg agaggctggt cggcgtggcg accttctcgc tgcctgccgg caccgcgctg
     5281 gctgcaacgg ctggggctgt ggccggcatc atcagcggct tgaccccgct cttggcaggg
     5341 atctcgatcg gaatatcgac cgcgaccggc cgtggctggg tgtcgaacaa cagggggaaa
     5401 ccgatcaccc cggccagcac cagcaccacc gcacctatca ggcggtgctt ggcgcgtttg
     5461 cgcatcactt ccacactctc ggtctgggcg gcagacccgg cagcagcaga aggttggccc
     5521 cggcgaaagt tgaaaaacgg catggcgatg acggtatctt agggcctgtt cacactattt
     5581 atgcaagtgc gaacgtggtt aaaaaagcgc caatctaggc gcgcgacgac gctcagcctg
     5641 ggcggctggg ctaggagtgc aacgacgagt ggtgttgttt taaccacgtt ccctccgggt
     5701 tgcggccaaa aaggcca
//
LOCUS       DAIDFG010000201         5712 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_201_length_5712_cov_15.765954, whole genome shotgun sequence.
ACCESSION   DAIDFG010000201 DAIDFG010000000
VERSION     DAIDFG010000201.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201_length_5712_cov_15.765954"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          914 a         1958 c         1884 g          956 t
ORIGIN      
        1 gaccatcagc cgcgaacgcc gcttcaccag cgatgctgcg catgaattgc gcacgccgct
       61 caccgcgata aaaacgcatc tccaggtcac gcgcatcacc cagggcaagg acgccgaggt
      121 ggccatggat tacgctgaag agggtgtggc gcggcttcaa cacacccttt cccagctgtt
      181 gacgctgtcg caggtcgagg gcgccttttc gtgggaggac ggatcggtcg ctgatgccag
      241 cgaagtggcc cgcctcgcga tgcgcgatgc agcaccccat gcgcccgagc ggatcacgct
      301 cgacagcgac ggcatccgtg ccgagctggc gctgccccag gcgctggctg tcaccgcgct
      361 gcgcaacctg cttgacaacg ccctgcgcca ttcccctgca tccaacagcg tcaggctgga
      421 aatacgcagc tcgccgcaag cggtcagctt tcacatattt gatcgcgggc cgggactcag
      481 cgacaacgaa ttggcactgg cgacgcaacg attctggcgg cgccggtcag gccggggcag
      541 cggcctgggc ctgtccatcg tcgccgccat tgtcgagcgt ttttccggat cgtttgaact
      601 gcggcggcgc tccgacggcg gactggaggc ccgactgacg cttccgcgca ttcaacccgc
      661 atcgccaaaa caggccccca ctgtctgaaa aacctgaaaa ccgatgcgct tcagacacct
      721 gccgcgatct gccgcaaggc ctgctcgaac acctcggccg gctggccgcc cgagatcagg
      781 tgtttgtcgt tgatgatgac ggccggcacc gcgtggatgc cctgctgcag gtagaactgc
      841 tcgcgctcac gcacggcgtc ggcgtactcg ttcgaggcca gaatggcttt cgcgcgcgcc
      901 atgtccaggc cgacctcgcc ggccacgcgc gccagcacct cgtgcgagcc cgggctttcg
      961 ccgtcggtga aataggcctt gaacagcgcc tccttgagcg ccagctggcg cccttctcct
     1021 tcttcctcgg cccaatgcag caggcggtgg gcgtcaaagg tgttgtagat gcggctgcgt
     1081 ttgtccatgc tgaaggtgaa acccacgtcc tgcccgcgcg cccggatcat ctcgcggttg
     1141 cgctgcgact gctcgggcgt ggagccgtac ttttcggtca ggtgttcggc gatgtcctgg
     1201 ccttcgaccg gcatcttcgg gttcagctcg aaaggctgga aatgtatctc ggcctggact
     1261 tcgcctgcga cccggcccag cgcgtcttcg agtgacttga ggccgatcac gcaccagggg
     1321 caggacacgt cggagacgaa gtcgattttc attgctgtcg gtgccggggt gctcatgcgt
     1381 gtgtccttgt cagaggggtg ggaaatcagg ccaatggtag tgacaatcgg gcaaccccgc
     1441 aggccaccgg gctgataggt gaagaccctt ctgagggaaa ataccccaaa atgcgcggca
     1501 ggggccaagc tcctcccacc cgcttttgaa caggacacac catgctccaa ctccgcccca
     1561 actgcgaatg ctgcaacaaa gacctgccca acgagtcccc tgaggcccgc atctgcacct
     1621 tcgaatgcac cttctgcagc gattgcgcca gcagcaagct ccacggcatc tgccccaact
     1681 gcggcggcga actggtcacc cggccacgcc ggccggctga caaactggcc cgctacccgg
     1741 catcgaccga gcgtgtcttc aaaccggccg gttgcggagc cgcggcatga ccagcagcag
     1801 cctgctgctg tttgcagccg tcgcgctggc ggtgatcgcc acgcccggtc ccacggtgct
     1861 gctggcgctg atcaacggct cgcgttacgg cgttcgccgc tccgtgcccg gcatgttggg
     1921 ggccgtggcc tcggacttcg tgctggtcgg tgcggtggcg ctgggtctgg gcgcgctgtt
     1981 ggcggcttcg gagttctggt tctcggtgct caaatgggta ggggccctct acctggcctg
     2041 gctgggcgtg cgcatgctgc gctcgaaagg cggttttgaa ttgcccgccg gcgaggccga
     2101 tgccgaacca cgcaatggcc gggccatctt catgaaatcc tttctggtgg ccgccaccaa
     2161 ccccaagggt tatctgtttt gctccgccct gctgccgcag ttcatcgacc cggccgccgc
     2221 gcaggcgccg cagtacatca cgatcgcgct ggtgttcggg gggctggact tcaccgtgat
     2281 gctggcctat gccgtcatcg gcgcgcgggc gttgcggctg ctcaaggccg gtgccacacg
     2341 ctggctggac cgcctgtgcg gcggcgccct gctgggcctg gccggctcac tggctttcta
     2401 ccgccgcgcc gggaactcct gatttcatag cgtgttgcgc ccgtcctgat tgggctggtg
     2461 cctgtttttg ttccagatca aggccaggcc cgtctgaccg tcaaatagga cttgtctgat
     2521 agcagggcgc gccgcgcagg actatggtgg gtttctctaa cggagatgtc catgaaccgt
     2581 cccacctctg ccccgacgag ccgggaagtc cgcattccct gcggtgacac ctggctgtac
     2641 ggcgacctcg tcctgccggc cggggccaaa ggcctggtgc tgtttgccca tggcagcggc
     2701 agcggccggc acagcgcccg caaccgccag gtggcgcaaa aactccagca ggcgggcatt
     2761 gccaccctgc tgttcgacct gctcaccgta caggaagaac aggtcgacac ccacacccgc
     2821 gaacaccgtt tcgacatcgc cctgctgacc gggcgcatgc aggaggtcac cgagtgggtg
     2881 tctgcccagc cggacctgaa acacgcaccc atcggctact tcggcgccag cacgggcagc
     2941 gcggcggcgc tgattgccgc ggcccggctc ggcaaacgta ttgcggccgt ggtctcgcgc
     3001 gggggccgcc ccgacctggc cggcccggtg gcgctggcgg cggtcagcgc gcccaccctg
     3061 ctgattgtgg gcggcgacga cgaggaggtg attacgctca atgagcaggc ctacgcgcat
     3121 ttgcattgcg aaaaacggct ggcgatcgtg ccgggcgcca cccacctgtt cgaggaacgc
     3181 ggcaccctgg agcaggtggc cgaactggcg gccgactggt tcagtgcctg gctggctcct
     3241 gccaaacaca cgggggttca ccatgagaga cacacgcctt ccctatgagg accgccgcga
     3301 ggcgggccgc gtgctggccg gcgcgctggc gcactaccgc ggtcgccccg gcgtgctggt
     3361 gctggccctg ccgcgcggcg gcgtgccggt ggcttacgag gtggcccgcg cgctgaaggc
     3421 gccgctggat gtgtttgtgg tgcgcaagct cggctttccg ggccacgagg aatacgccat
     3481 gggggcgatt gccagcggcg gcgtgcgcgt gatgaacccg atgccgggca tgcacgtcac
     3541 gcccgaagag gtggccgccg cggtggcgcg cgaacagccc gagttgcagc ggcgcgagca
     3601 gctctaccgc ggccagagcc cggcgcccga tctgcacggc cgcacggtga tcgtggttga
     3661 cgacgggctg gccaccggcg cgaccatgca cgccgcgctg ctggccatcc gccagcagca
     3721 accggcgcat ctggtgatgg ccgtgccggt gggcgccgcg gacagctgcg acgcgctgca
     3781 gggtgtggcc gatgaggtgg tctgcgtcgc catgcccgaa ccgtttcgcg ccgttggcct
     3841 gtggtaccgg cagtttcccc agaccagcga cgacgaggtg cgcacgctgc ttgacgacgc
     3901 ccgcgtgcac gcgccttccc cctgacaacc caccaggacc ctgccatgac cccgcagcat
     3961 tccaccctgc acaccgatac ggccctgctc gagggcctgc ggcagcacct gtaccccctg
     4021 actggcagca cgcacgacca cgacggcttg ctgcagatga tcggcccggc gcggtttgtg
     4081 ctgctcggtg aagcctcgca cggcacgcgc gagttctacc aggagcgcgt caacatcacc
     4141 aagcggctca tcatcgaaaa aggcttcacc gccatcgcgg tggaggccga ctggcccgac
     4201 gcctggcgcg tcaaccgtta tgtgcgcggc ctgtccgacg agcgcaacgc cggcgacgcc
     4261 ctgtcggggt ttgaacgttt tcccgcctgg atgtggcgca acaccgtggt gcgcgatttt
     4321 gtggagtggc tgcgcgagta caacgccggg cgcagcgctg ccagccaggt cggcttttac
     4381 ggcatggacc tgtacagcct gttcacctcc atccaggaag tgctggccta cctggaccac
     4441 accgacccgc cggcggcgca gcgggcgcgc gaccgctatg cctgtttcga ccacgcggcc
     4501 gaagacagcc aggcttacgg ttacgccgcc aatttcggcc tggcacgcag ttgcgaagac
     4561 gaggtggtgc agcaactgcg cgccatgacc cggcgcttcg cccagttgcc gtccacgccc
     4621 gggctggaac gcgacgagat gttttacgcg cagcagaacg cacgcctggt gcgcaatgcc
     4681 gaggagtact accgcaccat gttccacggc cgtgtgtcgt cctggaatct gcgcgacagc
     4741 cacatggaag aaacgctgca ggcgctggac cgccacctgg gcgccggcgg cgagccgccg
     4801 cgcatggccg tctgggcgca caactcgcac ctcggcgatg cggccgccac cgaaatgggt
     4861 gaccgcgggg aatggaatgt gggccagctc gtgcgcgacc gccatgacgg cgacgcggtg
     4921 ctggtgggct tcagcaccca ccacggttcg gtgacggccg cgtcgaactg ggacgaaccg
     4981 ccgcagcaca agcgggtgcg cgccggcctg cccggcagct gggaggcagt cttccaccag
     5041 accggtacca gccgcttctg gctgaacctg cgcgacaacg ccgcgctggg cgagctggcg
     5101 cagccgcagc ggctgcagcg cgccatcggc gtgatctacc ggcccgacac cgaacgccag
     5161 agccactact ttcacaccca cttgagccag cagttcgacg cgctgatcca catcgacgaa
     5221 acccatgcgc tggaaccgct ggaccggggc ccggtgtggc ataccggcga ggcacccgag
     5281 accttcccgt cggggatgta ggttttttgg ccttaatgga aatcgcggct ggaggtgccc
     5341 agccgcccca gcagcggcgt caggtcttcg agccggtgcg ccaccaggtt gcgcacagcg
     5401 ccataccctt cccgggtgtc gctttcctcg ctgcgctgcc agacgccgta caccgccagc
     5461 aggcgcgcat gcagcacctc gtggcgctgg gtgtcgcgca gtgatttcca gacaatcacg
     5521 ttgaccgggc ctgtctcgtc ttcgagcgtc acgaagatgg tgcccttggc ggtttgcggc
     5581 tgctggcgca ctgtgacaat gccgcaggcc gccacctccc ggccgctggg cagggcattg
     5641 agctcaccgg cactcagcag gccgcggcgg gccaggcgcg ggcgcagcag ggccagcgga
     5701 tggcgacgca gc
//
LOCUS       DAIDFG010000202         5673 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_202_length_5673_cov_17.488430, whole genome shotgun sequence.
ACCESSION   DAIDFG010000202 DAIDFG010000000
VERSION     DAIDFG010000202.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202_length_5673_cov_17.488430"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          904 a         1860 c         1862 g         1047 t
ORIGIN      
        1 gccgcagacc tcgatgcagg acgcgcaaca tgccaaggtc aggcgcgcgc tgtgcatggc
       61 aggcgtgaag cccggcgacc gcgtgctgga aatcggctgc ggctgggggg cgctggccga
      121 aatggctgcc accgaatttc gcgcctccgt cgtcggcgtg acgctgagca ccgagcaact
      181 cgcctgggcg cagcagcgca tggcccgggc gggcgcggcc gacaaggccg atctgcgcct
      241 gcaggactac cgcgacatcg gcaaaaccag cggggatgcg ccgtttgatg cgatctgctc
      301 gattgaaatg gtggaagccg tcggacgtga atactggccc acctacttcc aggcggtggc
      361 gcgtctgctc aagcccggcg gctacgcctg cgtgcagagc atcgtgattg ccgacgagtt
      421 gttcgagcgt tacgtctcgt ccaccgactt catccagcaa tacatctttc cgggcggctg
      481 cctgccgtgt ccgaccgagt ttcgtgccca ggcccgcgcc gccgggctgg aagtggtgga
      541 cgaattttcg tttggccagg actatgccca cacgctgcgg ctgtggcgcg aacagttcat
      601 ggcgcaagag tcacgggtgc tccagctcgg gtttgacaag aggttcatcc gcatatggga
      661 gttctatctg gcctactgcg aagcggcctt cgcccaggcc aacaccgacg tggtgcagta
      721 cacgctgcgc aagatctgac gggcgtactg ttgcttgcgc tggtgctggg catcggcgcc
      781 gccgcgccgg ctgccgcgca gaccggcacc gaagccgtgg cgggcagcgc caggccaccg
      841 gaattgctcg aagccctgcc gcagggcagg ctgctgggcg gcggccgcct gcgggttttc
      901 ggttttcagg tctacgacgc gcggctgtgg gcggggcccg gcttccgggt ggacaacttt
      961 gccagccatg cgttggcgct ggaactcagc tacctgcgcg cctttgaagg cggggcgatc
     1021 gccgaacgtt cgatcacgga aatgcgccgc gccgcgccgg tcagcgacga cctcgcggac
     1081 cagtggtcgg ccgcgctgcg cagggtgctg cccgatgtca agaagggcga tcgcatcatg
     1141 ggtgtgcacc ggcccggggt gggtgcgacc ttctggatga acggcaagcc gctgggcgag
     1201 atccgcgacg cggaattcgc caggcgcttc ttcggcatct ggctggcacc cggcacctcg
     1261 gagcccggcc tgcgcaatgc gctgatggcc ggggcaggcc cgtgaacact gctgccccgg
     1321 gcgcgtttgg cacccgcaac ggcctggcct acggcctgct gggcctgccg ttggcttttg
     1381 tggcgctgcc gctgtatgtg atcctgccca accactacgc ccgcgaattc ggtgtgccgc
     1441 tggctaccct gggcgcggtg ctgctgggtg cgcgcctgtt cgatgcgctg atcgatcccc
     1501 tgctgggccg tctcagtgac cgcctgtttg cacgctcggc cagggcggtg ctggggctgg
     1561 gcgccgttgc cgcgctggtg ctggcgctcg gcttcgcgct gctgtttttt ccgctggtga
     1621 gcgcaccgtc ggcactggtg gtctgggcca gcctgatgtt gatgctgacc tatgccggct
     1681 acagcgcgct cagcgtgtcg catcagtcct ggggcgcgat gctgggcggc gacgagcgcc
     1741 agcgcagccg catcgtggcc tggcgcgagg gcctgggcct ggttggcgtg gtgcttgcat
     1801 cgatcacgcc ggtggtgttc ggactgccgg ccagcaccgc gctgtttttt gtcgccctga
     1861 cggccggctg gctggcctgg gcacgcgccg accggccggt gccgcggcag acgaatgccg
     1921 gtccccatgt ggcgacgtcg atctggttac cgtttcgcca gccgggcttt cgccgcctgc
     1981 tcgtggtctt tctgctcaac ggcacggcca gcgcggtgcc ggcgaccctg gtgctgtttt
     2041 tcatccagga ccggctgcag gcgccgtcca gccaggagcc gctgtttctg ggcagctatt
     2101 tcgtgagcgc ggccctgtcc atcccgctgt ggctggcgct ggtcaaacgc atcgggctgg
     2161 cgcccacctg gctggtgggc atgctggtgg cgattgccac cttcggctgg gccacacaga
     2221 tagccgccgg ggataccgtg gccttcgtgg tcgtctgcgc gctgtccggc atcgcactgg
     2281 gcaccgacct ggcgctgccc ggcgcgctgc tggcgggcgt gatccaggcc aacggccaat
     2341 cgggccgggc cgaaggcgct tacttcggct ggtggaattt cgccatcaag ctcaacctgg
     2401 cactcgccgc cggcctggcc ctgccgctgc tcgccctgtt cggctacgcc ccgggggcgc
     2461 gcgacgcgca ggcgctcagc gcgctgctgg tggcgtattg cgtgctgccc tgcctgctca
     2521 agctggcggc tgccgcctgc ctgtattttc ttgtcatcaa acacccgcaa ggggtcccac
     2581 catgaaacac tttctccaca ggagcgcaac cgcgctcgcg ctgtcccttc ccctgctggc
     2641 cggtctggcg ggctgcgccg gcccgcagat caacgactac gccgccgaaa aaccggtgct
     2701 cgacatgcgc cggtatttca acggcacggt cgatgcgtac ggcgtcttca ccgaccgctc
     2761 cggcaaggtc gtcaaacgtt tcaccgtggt catgacctgc agctggcaag gcccgccggg
     2821 cgccgagacc ggcgtgctcg acgaagcctt cacctactcc gacggcagca cggaccgccg
     2881 ggtctggacg ctcaaacgca cgcccgacgg ccgctacacc ggcaccgccg ccgacgtgct
     2941 gggggaagcg gctggcgaag agaagggcaa tgccttccgc tggggctaca cactcaagct
     3001 gccggtggac ggccgcgtga tcgaggtgca gttcgacgac tggatgtacc tgatgaacga
     3061 caaggtgctg ctcaacaagg cggtgatgag caagttcggc gtgcggctcg gcgaggtcac
     3121 gctctcgttc gtgaagcgct gaccacacct tcctgattca agtcaaatcg gcctgtatcc
     3181 ctttatccac ctgcgccaac agctatcatt tttataacaa agcagcacca tgccgtacaa
     3241 ccctcccctt cgtgactggc acggcaaaac cgtgtggctg gtcggcgcct ccagcggcat
     3301 tggccacgcc acggcgcatg cgctgcacga ggctggcgcc aaagtcgtgg tctctgctcg
     3361 aaacggcgcg gcgctggaag cctttgtggc cgcccacccc ggcgcgcagg ccttgatgct
     3421 cgacgcgtcg gatgcggccg ccgtgaaaac cgcggcgcag tccctgctgg cccagggccc
     3481 gctggacctg atgatgtatt gcgcgggcta ctacagggaa caacgcgcca ccggttttga
     3541 cctggcggac atgctcaggc accagcaggt caactacgtg ggggcgctgt atgtgctcga
     3601 cgcggtgttg ccggcgatgc tgcaacgcgg cagcggtcac ctcagcctgg tcggcagcgt
     3661 cgccggctac cgtggcctgc cgcaaagcct ggcctacggc ccgaccaagg cggcgctgat
     3721 caacctggcc gaaaccctgt acctggacct gcaggccagt ggcgtggggg tgagcttggt
     3781 caacccgggc tttgtcgaaa caccgctgac cgccggcaac cagttcagca tgccggccct
     3841 gatcagcccg gcccaggcgg cgacggagat tctggccggc tgggcgcggg gcgagttcga
     3901 gatccacttt ccgaaacggt tcacgctctg gatgaaaacc ctgcgcgtcc tgccctacgc
     3961 gctgtatttc ccggcgatcc ggaaattcac cggcttgtga gcaccccatg atgaataccc
     4021 gggtcgagcc actggtgcaa ttttttgaaa ccctgagtcc ggctggcgtt gcgcgcatgg
     4081 gcgagttcta tgcgaccgac gcctggttca aggacccgtt caacgaggtg cagggcctgg
     4141 cgcaggtgca gcagatcttc agccacatgt acgtggcact ggaccggccg cgttttgtgg
     4201 tgacggcgca ggtggtcgac ggcagtcagt gtttcctgac ctggaacttc gagttttatt
     4261 tcaggcggcg cgggccacag cagttgcaga ccattcgcgg cgcctcgcac atcagcttca
     4321 acgaggccgg cctggtggcg cgccaccgcg actactggga cgcggccgaa gagctgtatg
     4381 aaaagctgcc gctattgggc agcctgatgc gctggctgaa aaaacaagcc cgtaaatagc
     4441 cctcacggtc attcacccaa gcgaccgctc ctgtttttat agctgctagc gcccgccaac
     4501 aaagggctac agaccttttt aggtccgaac cgcttcagct gggcagagtg tcgacaaaac
     4561 tcggacgctg cgccagtttg tcgtacagcc gggtcaggtt ggggtggggc gtgcgccagt
     4621 cgatctgcgg aaaacggaag tccaggtaac cgagcgcgca gccgacggcg atgtcggaca
     4681 ggctcaggtg gatgccgctg caaaaaggct tgtcgcccag accggtgctc atggcccgca
     4741 cggtcgtgtc gatcttgtcc atctggcggt caatccaggc ctgacaacgc tgcttgtcgt
     4801 cacgcccggc ccaagtggcc tccagccggg ccaggatggc cgcgtcgagc aggccgtcgg
     4861 ccagcgcttc ccaggtcttc acctcggcgc gttcgcggcc ctgcaccgga atcagcttgc
     4921 cgaccggcga cagggtatcc aggtactcca cgatcacacg cgagtcgaaa acggcttctt
     4981 ccgacccgtt ggcgccttcc atgaccagac agggcacctt gcccagcgga ttggattcgg
     5041 acatggtcgt gcccgcagcc cagacgtctt caatgacgaa ctggtagtcg agcttttttt
     5101 ccgccatcac gatgcgcact ttgcgcacat aagggctgcc gtaggatcca atgagtttca
     5161 tagtcaatct tttgtcgtga gccctggggc ttggtggctt ggggggtcgc gataccttaa
     5221 gccaactagc ttagcaggcc ccagccgatt gcattctatc gagtatgcgt cggccgggcc
     5281 tgtcacgcgg caaaaacggg gtaacctgtc cgccggggcg ccaggccacc cgcctacaat
     5341 gtcgcccatg aacccgtcca ccagcttctc catcaccgcc ctgtctcccc tggacggccg
     5401 ttacgccgcc aaactcgccc ccttgcggcc gctgatgtcg gagctcggct acatgcaccg
     5461 ccgcgtccag gtggaagtcg cgtggttcat cgcgctgtcg gatgcgggtt ttgcccaatt
     5521 caagccgctg acccccggcg cgcgcaccta cctgctcgga ctggtcaaac atttctcgga
     5581 aaatgatgcg gccgcgatca aggacatcga aaaaaccacc aaccacgacg tgaaggcggt
     5641 ggaatactgg atcaaatcga agttcgaggc gcg
//
LOCUS       DAIDFG010000203         5636 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_203_length_5636_cov_16.113600, whole genome shotgun sequence.
ACCESSION   DAIDFG010000203 DAIDFG010000000
VERSION     DAIDFG010000203.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203_length_5636_cov_16.113600"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1136 a         1767 c         1760 g          973 t
ORIGIN      
        1 tggccgcact gcggcgcgcg cgccgctcgc tgccggcgcc caacctggcc ggcctgccgg
       61 cagcaagctt gagccggcgc gaaatggagg tggcgcgctg cgtctgccgc ggcctgaccg
      121 acaaggaaat tgcacgcgaa ctcgcgatcg ccctgcccag cgtgcgcacc tacctgcagc
      181 gtctgttcga caaactcggc gtgcaccgcc gcgccggtct ggcgcagctg gcggaccgcc
      241 tgcattgagg cggcctggcc ggcattcgac aaaaatagcc tgcagccctt gtcagacaag
      301 cgccaacagc tatcataaaa atagcaaatc gaaacaaagc cgccgacgcg ggccgcccgc
      361 ccgtggccac catgccggcg ccgcgcgacc cccgtggctt gtggaatacg ccccgacatg
      421 cgaaaacggc ggtattactg acatgagttt gcgtctgcag gggctgcgcc agggcggata
      481 atcaaggccc cgacacggag agagaacccc ttgttttcaa tgcgtcaaga ccgctggatc
      541 agcctgatcg gcgggatcat ctttctggtg accaccctgg cagcgggcat cgccgtgtac
      601 gtcgtcatgg agcgacagac ggagtctgtt ctcagccgcg gcctgcaggc agcgctgcaa
      661 agcaacgccc gcctgtttga aagccagatc aaccaggccc tgaccgatgc gcgcaccgtg
      721 gcaacgcgtc cttttctgat gcagaccctg aaaacgttcg gggccaagcc ggacgatgcc
      781 gcggcaacgc tggcgatgca gcaggcggcc acctcgtttc tgcccaccgg tttcaacggt
      841 ttgtcctttc atgacgcgcg gggccgtgaa gtgacccgcg ccggcagttt ttcgcccagt
      901 ggcgagcaac aggtcctgct gagccagaag gaccgcgcat tcctgctgtg gaacggccaa
      961 ttcattgtcc agacgcactt ggacattctc gacccccagg gccttcgcgt gggcgaggtc
     1021 aagacggaga ccgccctgcc cttgttgacc cgggtctttg gcgatatggc caccataggc
     1081 cagagtgccg agttcgcggt ctgcgcaccg ctggacgacg gcggcaagga catgaactgt
     1141 tttctggggc gcgtgtccgg tacgtcgttc aagcgcgttg cgcgcgtcgt cgagggccag
     1201 gccctgccca tgaaccacgc cctgctcggc aacaccggca tcatcgttgc ccgggactac
     1261 cggcgcgaac aggtggtggc ggcctacgcg ccgattgcgc ctttcgcttt cgggatggtg
     1321 ctcaaaatcg accagaagga gctgtacgcg ccgatctacg cgcagctcaa gttcatcctg
     1381 cccctgctgg cagcgctggc aatcattggc atgctgctgc tcaatgttct ggtcgcgccg
     1441 ctggtgcgca aactggttct ctccgaaaaa gccgcgctgg atgccaacgc ccagctgcgc
     1501 aacagcgaaa cgcagttgcg cgacatcacc gacaccttgc cggccctgct ttcctacgtc
     1561 gatgcggatg ggcgcttccg ctttcacaac cgggcctatg aagaggcgct gggactgacc
     1621 tcggagcaga tcgacgggaa acacctgcgc gaagtcctgg gcgagaagat ctatcaagcc
     1681 acgatccccc aggttgaaca ggtcatggcg ggttacccgg tggtgtacga acgcatccgg
     1741 aacaaccttc gcggcgaccc gcgtgactat gtcgtcaact acttcccgcg ttatggcgaa
     1801 ggcaaggacg agggccgggt catcggcttt tattcgctgg caaccgacat caccgaactc
     1861 aagcgcctgg accgcatcaa gagcgaattc gtctccaccg tgagccatga attgcgcaca
     1921 ccgctgacct ccattcgcgg ctcgctgggc ctgatcgccg gcggcgtggc cgggcagctg
     1981 cccgacgccg tcaagtcgct ggtggagatt gccaagaaca actgcgaacg cctgatacgg
     2041 ctgatcaacg acatcctgga catcgagaaa atcgagtccg gcaagatgca gctcgacctg
     2101 caggtgctgg cgctcaaacc cctgctggag caggcgctcg cggccaacga aggttttggc
     2161 gccgcaaaaa acgtgcgcct gcgcctggtt tgccccgacg agaccttgca ggtgcacgcg
     2221 gacagcgacc gcctgaccca ggtcgtgacc aacctgctgt ccaacgcgat gaagttttcg
     2281 ccgcccgacg agacggtgga agtgcatgta tcgcgcgccg gcacgggcgt gcgggtcgag
     2341 gtgcgcgacc acggccccgg cattcccgac gagtttcgca agcgtatctt ccagaagttc
     2401 tcgcaggccg actcgtccga cacgcgccag aacagcggta ccgggctggg cctgaatatt
     2461 tcgagggcga tcatcgaacg cctgggcggc agcatgggtt tcgagagtga agcgggaacg
     2521 ggcaccacct ttttctttga gctgccgcag gcgccatggg cgcaacccga cgaccagccc
     2581 gtggcaacgc ttgccgccgg cacgccggcc agcacccgtc cgcgcattct cgtgtgcgaa
     2641 gacgaccgcg acattgccca cctgatcggc ctgatgctcg aaaaaggcgg ttttgacgtg
     2701 gaccgggcct acaccgccgc catggcactg gcctttctgg agaaaaacag ctacgcagcg
     2761 atgacggtgg acctgggtct gcccgatcag agcggggtca cgctgatccg caccttgcgc
     2821 gggcaagcgc acacgcgcga cctgcccatc gtcgtggtgt cggccgcggc cgcggaaggc
     2881 cagatcgagt gcaacagcct gccgctgaac gtctcgaact ggctggacaa accgatcaac
     2941 gagaaccagc tgatcctcgc acttcgcctc gccatcgacc gcatggccaa aggcaagccg
     3001 cgcatcctgc atgtcgagga cgacttcgac atgcagcgca tcacggccgc catcgcccag
     3061 gactttgccg tcttcgagtt tgccgcctcg cccgaggaag cgcgtgcgcg gctgcgggag
     3121 cggcacttcg acctggtgct gctggacctg gccttgcgtg gtgcgtcggg ctgggaggtg
     3181 atggcagaca tcgaggcgct ggacaagccg ccgccggtgg tggtgttctc cgccacggaa
     3241 ctgaaccgtg ccaaggccgc ccggttcgct gccgtgctgg tcaaagccca gaccacgaac
     3301 gccgaagtgc tgcagatcct gcagcgcgtg ctgaccgagg cgcatccggc tggcccggcg
     3361 ccggcctgag cgaacggcct gtcgccaggg cctgtcgggg ttcacggagt ttccgcgccg
     3421 cgcgtcagat actcctgaat tgatagctgc cggcgcccgc cagacaagag ctggataccg
     3481 tttttccttg caacctgaag cgcagcccga aaatctcagc cctgctggag cagcgtgtcg
     3541 atctgctggt ccttgtgctc ccacaggtcg ccgagccact gctggaaaga catgcgaaac
     3601 gccggatcgg cggcgtagtc accctcgctg aactcggggg gaatcggcag ttgctgcgcg
     3661 cgcacgatga tgcgcggcgc ctggccgcag agaaactgcc agaaggacgg cgcacccgcg
     3721 gggtagacga tggtcacgtc aatcagcgag tggaattttt cgcccatggc gttgacggcc
     3781 agcgccagcg cgccggcctt gggcttgagc aggtgccggt agggcgaggc ttgcgacgca
     3841 tgtttggcgg gcgtgaaacg ggtgccctcg gcgaagttca tcacgctggt cggcaccagc
     3901 gcaaacttct cgcaggcacg tttggtggcc tcgcggtcct gcaggcggcg ctcggggtgt
     3961 ttgcgcaggt cggccttggt gtggcgcttc atgaagggaa agtccagcgc ccaccaggcc
     4021 aggccgatga ccggcaccca gatcagctgc tgcttgagga aaaacttcag catgggaatg
     4081 cgccggttca gcacatgctg cagcacaaaa atgtccaccc aggactggtg gttggcattg
     4141 accaggtacc agccttcgta acgcagggcg tcaacgccgg ccacgtccca cacggtacgc
     4201 tgggtcagcc gcatccagcc gctgttgcag gcgatccagg cggtggcaat cgcgttgagc
     4261 agcgggtcga tgcgcacacg caccgccttg aaaggcagca ccagcttgac cagcgacgcg
     4321 gcgaacagca gcgtgcacca gaacagcgtg ttgacgacca gcagacacag cgcaatcacg
     4381 ccacgaagaa aggaaggcag aagtccgagc atgggcagtt caaagcctga attttcgcct
     4441 gcgcgggcct gcccggcctt gtggaaggtc aaaggcaccg cgatggtgtg actggaacgt
     4501 gccgcagtgc tacgttttta atagctggtt acgcccgccg gataagggct aacggatgaa
     4561 ttcagtcgaa aacatgaggc ccgtttccac tctgcgaggg gtcagacctc acctgcgcag
     4621 ctcggcggca aggccttgca gcatgctgac tctgcgaaac gtggttcgga gacgcagccg
     4681 gcgctcgaga acagcattgg agaacttgct ctccatttgc ctgccctggc ataaccagta
     4741 gacctgagcc ccttcgtgga caaagtcgtc cagcgagctt cgcagcaagg ccagttcgcc
     4801 ggattgagct ggagtgagtg gctcacgcag aaaggcaaca ttgacctcgc ctgattgtgg
     4861 aacctgctcg cgcaaatgca gcgctcttgc agcaacagcc tcaacctcgg ccgcggttct
     4921 gatgaacacc tcggacgtga aacccagaag aggagcgaga cccacttcaa cagcggtggc
     4981 caacttctgg gaagaacgtg aacgggccgt gaagatgaca tttccggagt tgatgtacgt
     5041 cttcgcgtcg tcatggccga gcgtacggaa gtgctccgcc agcgcagaca tcttgatgaa
     5101 gcgcccggtg acgttgatgg ctcggaggaa ggctacgtgg actggcacgg gtgttttggg
     5161 aggtctaatg tttgagataa gcggatgccg aaggcaatcc gcacttgacc gaaatgttag
     5221 ccagcattgt tcgcgactcg cagaagaaca gctgcgacca ctagtagcgg cgtaccaact
     5281 gcaatgtgcc aaaactcggc cccgagaccc acgccgatcg cgccaatacc tccgataagc
     5341 gctgccaaaa cgtacgcata agtacgaatc agcatgagag gcttatcgcg tgaatgctct
     5401 tccatgatgg ctaacgtaat gtagaccgca aaacctgcgg tgtaacttgg cggctgtgga
     5461 ttaatccggg aaacccggca gcgtaagtgc ttgtcgggat tcggttttca gacatctatt
     5521 gtttaccccg cacaattaaa tggaaaatgg aaccacaaac cccacaaagg gaaattgtaa
     5581 tgaccgcccg ggagaaggta gctcagacac gccgacccag cgcaccactg gccaac
//
LOCUS       DAIDFG010000204         5526 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_204_length_5526_cov_16.348382, whole genome shotgun sequence.
ACCESSION   DAIDFG010000204 DAIDFG010000000
VERSION     DAIDFG010000204.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204_length_5526_cov_16.348382"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1003 a         1649 c         1689 g         1185 t
ORIGIN      
        1 gggacagagt gattgcgtca ttgctaatgg cgtcgatctt ggcttggcct gtatgtcgtg
       61 gtgcgctgcc ggaggcaggg ggtttcggca cctcgttgag ccgtgcctcc acgcctttga
      121 ggcttgcttc cgagtcgatc aggaactgcg acgacaccac cacccgctgg cccgtctgca
      181 ggccgcgctt gatttccgtc tggccgccgc tttcaatacc tgtttcgaca tcaacgggcc
      241 ggaagcgtcc gttctcctcg gccagcatca cgaccgtgcg tttgccggtc aggatcacgg
      301 cttctgtagg gatcagcaag gacttctcag cccgcatgtc catgaactgc atggtcacga
      361 acatgcccgg taccaggcgt gagcccgggt tcgccagttc caatcgagct ttgagcgtgc
      421 gcgtcgccgc attgacgtcc ggcaggatgg cttgcacctt gccttcgaag gtcgtcccgg
      481 gcacggccgg actgcgggct tgcaccttgg cacccggtcg caacagcgcc gcctggcttt
      541 cgggtacctc ggcgttggcc catacggaag aaagcccatt gatgcggaac agggtcattc
      601 ccgccgacac cgtcatgccc tcgcgtacca gcagttccac caccacgccg cccaatggcg
      661 ccgtgatcgt agtgcgagcc tgcgttttcc cgctggtctc caccaggcgg atttgctcgt
      721 cgctcatgcc gacctggcgc atacgctggc gggcgccgtc gaccagcgaa ccgaggtcat
      781 tcccctgcat gcgtttcagg gacaaaaatt cctcctgagc cgccacccag tctggaacat
      841 acaggtccac caaaggctga cccttggcta ccggatccag cgtggctcgc acatgcaagc
      901 gctcgacata gccggtcgcc cgggcttgca ccgtggcctg gttgcgttcg ttgaacgcaa
      961 tgttgccgac cgcagagact tgaggcgaca gcgtgccttc ggtgactact gtggtgcgca
     1021 cccccaggtt ctgctgaatc cgggagctaa cggtgacctt gccctggtcg gaatcgctgt
     1081 ctgcatagac cggcaccagc atcatgtcca tgaacgggga cttggcgggt ttgtcgaact
     1141 tgttgcccgg catcataggg tcctggtagt acaggatttt tcgcccgctg acgggatcgg
     1201 tatcacccgc tttgagacct gcgctgatgt ggcggcgggt ggcttcttcg ccctcggcaa
     1261 cgctctgggg tccagctgta ggtgtcgccg ctggggtcgg cgacgcttca cctggaatgg
     1321 cggtggcggc catgtttccg ccgtttttca tgcccagggc gtacaggcca tagcctgcag
     1381 cgcccagtac accggcagcg atcagcgcgg caataaggta tttcttgttc atggctgttc
     1441 cttggcttgt gccggcatgc tgtgttcggg gacgaggaaa ttgagctggg cccaggaacg
     1501 cgcagtttcc atttccagcg tcagggcctg catgtggatg tcgagctcat ctcggcgcgc
     1561 ggccaatgcc ccggccaggt cgctcttgcc cgtgcggtag ctggtcagcg cggcctcggt
     1621 gcgctgccgg gctgtcggta tcagctcatt tcggtaccgg gtgacccggt ccttgcccgt
     1681 ttgccagtca ttcagccagc cacgcacctc ggcctcatgg ttacgcagca tgtcttcgta
     1741 gcgggccctg gcttcgtcga ccaaggccag cttggcggcc agctcgcggt tctggcggtt
     1801 cttctggtcc cactgcaagg ggatggagac gccgatggag atcatgttgg agtaagtcgg
     1861 accgcgctgg gcgtagctgg cttcgacgct ccagtcggct tgtttgttcg cttgggccag
     1921 ccgtgcatcg gtttccacag tgtcaatttc tgcacttatc gctcgcagat cagggtgttg
     1981 ctttatgtgc tcgcttgaaa ccccaatctg aagggaggtg gtttgccagg acggagagcc
     2041 ggagagcggt cgatccgcat ccgtcgtacc cacccaacgg gctagcatca ggccggcgtt
     2101 gcgtgactgc cggccaatct ggctgacccg gtcctccagc tggatcaccg cgctacgggc
     2161 ggcgaacacg tcggactggc tcccccggcc ggcgcgaaag gcgatgtcgg ctgcttgtat
     2221 ttgcagccgg gtttcttcga gctgttgctg caccagctcg cgcatggcct gggtgtaata
     2281 ccggtccaac caggccagcg cggtgtcgcg ctggaggttc accagcgtca gctggcgctg
     2341 cgcctgcact cgctgcgcat cgcgttcaaa gcgttccgag cgcagctggc gcttggcgga
     2401 acgggtgatt tcctgcatga cgccgatgcg gcgcatggtc atgaaatcgc gtgtcaggct
     2461 gccgcggtcc gcgccgtcgg ccggcaggtt gtcgatgccg agtttgagca ccgggtcggg
     2521 gagctggccg gccgccacgg cctgttcctt ggcggcggcg gtcagtgcct cttgggcgac
     2581 caattgctgg gatcgatcaa cggcaatgcg ctgcgcctcg atcagggtca aaggatcagc
     2641 agcccacgac gcagaagcga tggccaccat ccaggcggcg gccgtgatcc gggaaatcgg
     2701 aaaagaaata gacataaaac ctccagagca cgaacaacga cctgccgatg cacggggagg
     2761 tgcagcagac aggagccagg tggtccggga ggtcagaggc ggaagcagca gttccggatg
     2821 gcgacagggg gagcggtggt gcgctgcaga tgaggcgcct gcagcggcgc gtcagaaaac
     2881 accgggaggg caatctgcaa actgaagttg gcaggcaaag cgctgatggc aacgggggag
     2941 gacagctcat gcttgtcggc tgactgatga cccgcttggc aatgtgcctg gcacaggttg
     3001 ggttgctgat cgtccatgtt gagcggcatg gcttcggcgc agggcattcc ggcctcggac
     3061 atcgcggacg cttcggcgat ttttggttca gaaccgggac agatgtagcc tgccaccgcc
     3121 agctgcatga acagcaggct taccagtgcg aagagcactg tgatcagacg ttggcggcgg
     3181 tttcggatca ttgcttgagt ttaagggaca tgtgaccggt gaagcgctgg aaagttccgt
     3241 cttcggcgat cggtgcccga aaaccaatgc cgaccctagc ttatcaatcg gaacctaagt
     3301 tgagcttgcg atagccatgc gcgctcgttg aagccggtct aacccgcgtt tagggtaagc
     3361 acactcattt ttccagattt tctacgcctt cagcagacgg ccaattgact taccgcaaat
     3421 cagactcaca ctggcgctga aatagtccga gcccgtcggc ttcaggtcga cacccgttcg
     3481 tagataaacg tacaccgcgg cgcgcctctc gcaatcgtgt tgactgggga gttgccgcga
     3541 tggaagcgca tgatcggcga atactcgttg aaaaccgttt cgtctgaacg gcagaatgcc
     3601 agggtgatcg ccggattgcc catgtccctg aaacattcgt ggaaccgaca ccggacgatg
     3661 tcgagcgtga cccgggagtc cgagcgttgg atgccgtcgt tctcgttggt actgatgaaa
     3721 ccttcccgca tctgcgtttc gtgcgcatcg tggaagaaat cccaagttcg tgcctttccg
     3781 ccaggcggga agtacgcctt ccgctggacg gcacatccca ggtcgtgcag aaaggactgg
     3841 gttgtggaga gtgcccgctc ggcgccctga gccttcacca gttcgttata caggacgcca
     3901 accagcagga aagtgtcttt cacccgccgc agttgcgcgt cgtcgcctgg cttcgagaat
     3961 tgcttcttgt aagttcctgc gagagcgaag agtttcggaa tgaggacaaa gcgtcgccag
     4021 ccgagtaccg gctgcaagtg tcggttcaac agcttgggta ccgtgaacag gagggtgccc
     4081 aagcgacgcc gggcgtcaag ggcgggatcc agatgggcgt ctgttttcat ttttcggcgc
     4141 atgacgaact ctgtttcaat ccgcgtcccg atctgatgga cctgcgggtg cattgggtag
     4201 gtgtactcgt tttgctccgc agtacgaacg ggctcagcag tttcattgaa ttgatgggcc
     4261 tgccctggtg gccgtggccc gcgttttggt cgtggttcat acccacgcca gccagatgct
     4321 gttcttctcg ttgcatgatg tcgttctgcc tttgtggctc caattgtcgc tacgcacatc
     4381 cgaggagtag ccgtgatttc tgaaaaattc gaaaaaatta acctaagcta ttgatttaaa
     4441 agagtaaata tggggcgtct cagaaaaccg aaaataaagc acactaaggc gtagccaacc
     4501 cgtgactccc ccgcgccgac tcagcgagct tcgttccgtc ttgcagcgac gcgatcagag
     4561 ggcaggaaac gttccccttg cgcgcatggc aggcacacac caactcagac agcacggtct
     4621 ccatgcgcgc caagtcggtc attctcgcgc gcacatccgt gagcttgcgc tcggccaggc
     4681 tactggcttc cgagcaatgc gcaccatctt ccaatcgcag cagttcggca atttcatcca
     4741 gactgaaacc cagccgctgg gctgatttca cgaaccgcac ccgcgtgaca tccgcctcgc
     4801 catagcggcg gatgctgcca taaggcttgt cgggctctgg caacaacccc ttgcgctgat
     4861 agaaccggat tgtctccacg ttgacctcgg ccgccttggc aaaaacgccg atggtcagat
     4921 tctccaactc gcttttcata tcgcttgact ccgtacatga ctacggaagt aagcttacgc
     4981 catcccattc agattcgaaa ggaaacgcac atggctgaac cgcaaaatgg gcgcggggcg
     5041 ctcttcactg gagggctggc cgccatcctc gcgtccacct gctgccttgg gccgctggtg
     5101 ctgatcaccc tgggatttag cggagcatgg atcggcaacc tgacgacgct ggaaccctac
     5161 agtcccgttt tcattggcgt ggcactggtg gccatgtttt tcgcctggcg gcgcatcttc
     5221 cgtccggcgc aagcctgtaa accgggagac gtgtgcgcga ttcctcagat acgagcgatc
     5281 tacaagttcc ttttctgggt cgtggccgca ctggttatgg tcgcgctcgg attcccgtac
     5341 atcgcaccac ttttttattg accacaggag cccatcatga agaagttgtt ttccgccctt
     5401 gccctggttg ccgtcattgc gcccgtgtgg gctgctaccc agaccgtcac tctgtccgta
     5461 ccgggcatga cttgcgccgc ttgcccgatc accgtcaaga aggcaatttc caaggttgat
     5521 ggcgtg
//
LOCUS       DAIDFG010000205         5469 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_205_length_5469_cov_19.253786, whole genome shotgun sequence.
ACCESSION   DAIDFG010000205 DAIDFG010000000
VERSION     DAIDFG010000205.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205_length_5469_cov_19.253786"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          878 a         1875 c         1735 g          981 t
ORIGIN      
        1 tcagccacag ctccagcgtc ggccgcacaa aattcgagac caggcgcacc gcatcgcgcg
       61 aattgagccg ctccttgatc tgcccctgga attgcgggtc cagcaccttg gctgagagca
      121 cataactggc acgggcaaac acgtcctcgg gcagcagctt gacgcccttg ggcagcaacg
      181 aatgcagctc gatgaaactc ttgacggcct ggaacacgcc gtcgcgcagg ccgctttcgt
      241 gcgtgccgcc ggcgctggtc gggatcaggt tgacataact ctcgcgcacc ggctggccgt
      301 cttcggtgaa cgccaggcac cactgggcgc cctcgccttc ggcaaaactg tcgtgcgcgg
      361 catcggcaaa accttcgccc tcgaacagcg gaatcacagg atcggcgtta agcgtctgca
      421 tcaggtagtc gcgcaggcca cccttgtaga cccaggtctg cacctctttg gtcttttcga
      481 ccaccagcgt cacggtcacg ccgggcatca gcacggcctt gctgcgcagc aggtgggtca
      541 gctcggccat gggcagagcc gccgcctcga aatatttggc atccggccag gccctcaccg
      601 tggtgccgga cttgcgatca ccctcggccg ccttgcgcgt ctccagcggc tcggtgacgt
      661 cgccggcctc gaacaccagc ctggccacca tgccgtcgcg gtaggaggta gcttccaggc
      721 gtttggacag ggcgttggtc acgctgacgc cgacaccgtg caggccacct gaaaaactgt
      781 aggccccgcc cttgcccttg tcgaacttgc cgccggcatg caggcgggtg aaaaccagct
      841 cgatcaccgg cgccttttct tccgggtgca ggccgtaggg aatgccgcgg ccgtcgtctt
      901 cgatgctgac ggaaccatcg gtgtgcagcg tgaccttgat cttcttgccg tggccggcga
      961 gtgcctcgtc ggccgagttg tccagcactt cctggatgat gtgcagcgga ttgtcggtgc
     1021 gcgtgtacat gcccggccgc tgcttgacgg gctccaggcc cttgagcacg cggatcgagc
     1081 cttcggagta gtcgttgggt agtttggtcg ccatgggggg tggattgtag tcccggatga
     1141 ttttataact ggataaatat acagtttttt atccgccacg ttccgaaacc catgcctggc
     1201 gctcatcaat ggcgcgaaaa actttcaaag cagaacgcag tgccattggt tacattctcc
     1261 ggatgcaatc tccaagccga aatctctccc tggcccaagt gctcatctgc ggcgccgcca
     1321 tcgtcaccct gtccatgggc atacgccacg gcttcggcct gtggctgcaa ccaatcaccc
     1381 aggcccaggg ctggacacgc gaaacctttg cctttgccct ggccgtgcaa aacctgtcct
     1441 ggggcgtgtt tggcattttt gccggcatgc tggccgaccg cttcggcgcc tttcgcgtca
     1501 ttgcgggcgg ctcggtgctg tatgcactgg gactggtcgg catggcgctg tcgccgaccg
     1561 gcctgttgtt cacactgacg gccggcgtgc tgataggcgc ggcgcaggcg ggcaccacct
     1621 atgccgtgat ctacggcgtg atcggccgca acattccggc cgaaaaacgc tcctgggcca
     1681 tgggtgtggc ggccgcggcc ggctctttcg ggcagttcct gatggtgccg gtggagggtt
     1741 tcctgatcag cggcctgggc tggaaagagg cgctgctggt gctgggcgcc gcagtgctgc
     1801 tgatcgcccc gctggccttt ggcctgcgcg agacccgctt cggccccggc caggcgccgc
     1861 cgcgtgaaca gaccatcctg caggcgctca aggaagcttt caagtacccg agcttccagc
     1921 tgctgatggc cggctacttc gtctgtggct ttcaggtcgt gttcatcggg gtgcacatgc
     1981 ccagctacct caaggacaaa gggctgtcgc cgcaggttgc cagctacgcg ctggcactga
     2041 tcggcctgtt caatgtgttc ggcacctacg cggcgggcgc gctcggccag cggctgccca
     2101 aaaagaacat cctggccttt atctatctcg cgcgggccgt cgtcattgcc gtgttcctcg
     2161 ccgcgcccct gagcccggcc agcgtctaca tttttgccag cctgatgggc ctgctgtggc
     2221 tgtccaccgt gccgccaacc aacgccgtcg tggcgcagat tttcggcatc cagcatctgt
     2281 ccatgctcag cggttttgtg tttttcagcc accagatcgg ctccttcatg ggggtctggc
     2341 tgggcggcgt gctctacgac cgcaccggca gttacgacat cgtctggtac atcacgattg
     2401 cgctgggcgt gtttgcggcg ctgatcaacc tgccggtgcg cgaagccgcc atccaccggc
     2461 ctgccgcggg ccgcctgccc caaggcgcct gacatggcac gcaagctcgc cttgtacctg
     2521 ctcgccatca cggccacgct gggcgtgttt gcgctgtatt tgcgtcccga ttttttgctg
     2581 acgctggccg accagctctg ggcctgcttc taagcctgaa cctcagtcct cgtcgcgtac
     2641 ccggtactgc ccgaccagtt gctgctgcac cgccggcggc acgacctcgt agtggctgaa
     2701 ggccagcgtg taagccccct gcccgccggt cagcgcattg agccgcgact ggtaggcggc
     2761 cagttcggac agcggtacct ggcccagcac cgccagcgtg ccagcctgca gcccgcgcgt
     2821 gccgctgacc tgcccacggc gcgccgacag gtcgccggtg atgtcgccca tgacagcctc
     2881 gggcgccgtg atctcgacat cgaccacggg ctcgagcacc accggcgacg cggcgcgcac
     2941 cgcttcgatc accgccttgc gtccggccgt ggcgaaggca atgtccttgc tgtccacact
     3001 gtgatgtttg ccgtcgtaca ccacgacccg catatcgctg accggaaaac ccgcgatcac
     3061 cccgccgtcg agcgcctggc gcacgccttt ttccaccgcc ggcatgtact ggccgggaat
     3121 cgtgccgccc ctgacttggt cgacaaactc gatgccggcg ccgcgcgcca gcggctcgat
     3181 gcgcaggaag acctcgccga actgccccgc gccgccgctc tgctttttgt ggcggtggtg
     3241 gccggcggct ggcgtggtga ccgtctcgcg gtaggcgata cgcggcggcc gcgtgttcac
     3301 ctcgcacttg tagacctcgg tcagccgctc gatcagcgtg cgcaggtgca gctcgccgag
     3361 gccgtacacc acggtttcgt tggtctgtgc cacatgttcc agccgcaggc agggatcttc
     3421 atcgaccagc cgctgcacca ggtcccacat gcgctgctcg tccccgcggc gtctgggctc
     3481 gatggccagc ccgtgcaccg gcaccggaaa gtccagcggc tgcagatgaa tgtgttcgtc
     3541 ctcggcagcg tcgtgcagca cggcgtcgaa atggatctcg tcgaccttgg ccacggcaca
     3601 cagctcaccc ggcccggcgc tggcgatttc catgtggtcc ttgccctgca gccggaacag
     3661 gtggcccacc ttgaagggac gccgaccgtc gccgacgtag agctggctgt cacgcgtgac
     3721 cgtgccctgg tgcacgcgaa aaatgcccat cttgccgaca taagggtcca cgctgacctt
     3781 gaaaacatgg gccagcacat gacgcgccgg gtcgggctcg gctcgcaccg gaacagcggc
     3841 ggcaccccct ccgcgcaaaa acttcggcgg gttgccctcg gccggatcgg gcagcagcct
     3901 gaccatcacg tccagcagtt cggccacgcc ggcgcccgtg cgcgccgaca caaaacaaac
     3961 aggaatcagg tgcccctcgc gcagcgcctg ctccagcggc gcatgcagct cccggggatc
     4021 cacgtcgcct tcgtccagat aacgctcgac aaaagccgga tcgacctcga ccacctgctc
     4081 gaccagcgcg cggtgcgcca gcgccacgct ggaaaaatcg gattctccgg ccggattgaa
     4141 aaaacagtcc accacccgcg tgcccccgcc agcgggcagg ttcagcggca ggcactcctt
     4201 gccgaaggcg gcctggatgg cggccagcag gccgggcagg tcaatgccca gcgcgtcgat
     4261 cttgttgatg atcaccatgc ggcacaagcc gcggccggcg gcccagtcca tcatgcggcc
     4321 gctcatgagc tccagcccgc tggcagcgct gacgaccagg gctgcggttt ccaccgcctc
     4381 cagcgcgggc agcgactgcc cgagaaagtc cggcgcgccg ggtgtgtcga tgatgtgaat
     4441 gcgcgtgtcc tggtgctgca ggtggaccag cgccgaatgc agcgaatgct gggcgcggcg
     4501 ctccagcgga tcgaagtcgc taaccgtcgt gccgcgctcc aggctgccgg gggcgccaat
     4561 ggcgcccgcc ttgtgcagca aagcctcgac cagggtggtt ttgccgctgg ctgctgcgcc
     4621 caccaacgcc agcgtgcgca gggccgcgac ggccgacgga accggatgca tgggtgagga
     4681 catggcgcgc tcctgggtgc cgggcgtggc gtctgcgccc gagggattgg cctgacgcct
     4741 gaatcagacg tacagcgtaa gggatagccc gcaccggggc aagaggccgg agcagacatc
     4801 aaacggtggt agtctttcgc ctccacacac gcaggggaca gcagggccat gaaaaccatc
     4861 gtcatcaccg gggcttccga cggcataggc gcggaaatgg cgcgtcagct ggcgcagcgc
     4921 catggtgctg cggtggcgct ggtgctggcc gcgcgcaacg aagcagcgct caataccgtt
     4981 gcctcccagt gccaggccag cggggccgcg gcactggtgg tccccaccga tgtgtccgat
     5041 ccggcccagt gccgcgcgct ggtcagcacg gccgcccggg cgttcggcca gatcgacgcc
     5101 ctgatcaaca acgccggcat gtcggcgcag gccctgtttg aagatgtcaa aaccgacgac
     5161 ttgggctggt acgaagacct gatgcgcatc aacctctggg gcagcgtgtg gtgtacccat
     5221 gccgccctgc cctacctgaa gcaaagtcgc ggcagcctgg tggcggtctc cagcctcgcc
     5281 ggcctggtgg gtgtgcccgg gcgcacggcc tacagcgcca gcaaattcgc catgaccggt
     5341 ttttttgaag ccctgcgcgc cgaactgaaa acggccggcg tcagcgtcac caccgcttac
     5401 cccggcgtgg tcgccacaca gatccgctac cggggcttca acgccgcggg cgcgcccgcc
     5461 ggcgccagc
//
LOCUS       DAIDFG010000206         5450 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_206_length_5450_cov_18.151993, whole genome shotgun sequence.
ACCESSION   DAIDFG010000206 DAIDFG010000000
VERSION     DAIDFG010000206.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_206_length_5450_cov_18.151993"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          822 a         1790 c         1906 g          932 t
ORIGIN      
        1 ccctggctgt tcgagggccg cctgccggac ctcaacatcg gcacggcgaa tggcgcggcg
       61 gcggatgcct ccatcaccgc cgccgcggcc gctgcatgcg ccggggtcgc gggggtgagc
      121 agcatcgcca acggccgttt caagggcggc tacatcacgc ggcagtacgg ccggccggcc
      181 gggcatgtgc acgcggtcca gctcgagaaa tgccagagcc tgtacatgca ggaagtgacg
      241 ccgtttgcct acgacgaggt gttggcgcaa aggatacaac ccgtgctcaa gagcatggtg
      301 acggacagcc tggccgctgc ctgcaggcta tatggccgct gagacggtgt cttgagtcgc
      361 gccccttcga caggctcggc cccaacgggg cgtgtcatga atgacgaaac cgtcaagaga
      421 atacgaacac cgctgtgtgc ctgcatggat tgcggctcaa ggccgcaatg acaagtcagg
      481 cagggtggag tagcgcttca accgccgcgc ccagcgccag cagttcccgg tcctggccac
      541 gcttgcccac cagcatgagc ccgaccgcca gcccgccgcc gcgcggcagc ggcaaggtca
      601 acgccggcag gtcaaggaag ttgaagaccg aggggttgcg cagcagcagg gtattggtgc
      661 gcaaaaacgc gtcttcggcc tgtacctcgg cgatgcgtgg cgccagcagt ggcacggtgg
      721 gcagcaccag cgcatcgaga tctgtcaaac gcgcatcgag cgccggcagc agcgcggcac
      781 gggcctgctg gataccgacg taggtcgccg catccagtgt ctggccgcgg cggatgcggc
      841 ccagcacgat ggggtcgtac gcttcgggcc ggctatccac ccagtcgcgg tgaatccacg
      901 ccgcttcggc ggccaccagg gtgccgcgct cctgcaggcg aaagggttca cccagcaaat
      961 cgtcaagcgc ttgatcgcgg acccgggcac cggcgcccgc gagctgcgcc agggcctgct
     1021 ccaccgcggc cagcacctcg tcctgcgcct ggctgaacaa cagaccgcgc ggcacgccca
     1081 ggcgcaagcc gcgtacatcg cgctgcggca gggcttcatt cgcgatgccg gccagcacgc
     1141 tgtcggtggc ggcggcgtct gccacgctgc ggcacagcgg tcccagggtg tccagggtga
     1201 aggacagcgg aaaagcgcct tcgcgcgtga cccgcgcctg ggttggcttg aagcccacca
     1261 ggctgttgag cgccgatgga atgcgcaccg agccgccggt gtccgagccg atggcgatct
     1321 cggccatgcc gcgcgccacc gacacgcccg cgcccgagga cgagccgccc gggatgcggc
     1381 tgatgtccag cgcgttgccg ggcgtgccgt aatgcgggtt gatgccgacg cccgagaaag
     1441 cgaactcggt catgttggtt ttgccgatga tgacggcccc ggccgcgcgc agacggctga
     1501 ccaccacggc atctttggca gcaggcgcgg catcgcgcag cagggccgag ccggcggtgg
     1561 tggcttcgcc ggccacgtcg aacaggtcct tgatcgagac gatgcggccg tccagcgggc
     1621 ccagcggctg gccgctggcg gcgcgccggt cggcgaccag cgcggccgtg cgggcgggct
     1681 caaggtacag ccgggtgaaa atgcgcgggc cttcatcgcc ggcggctgca attgcggcca
     1741 gcgccgcttc gagtttctgg gtggaagtcg tcgtgttcat ggggcataac cttaacgcat
     1801 tgcggtggtg cttgccgcgc ggggcggact gcgctgcact gccggttgct atgaagttga
     1861 tagctgtctg cgtccgctta tcaagggctg gaggcacttt ttgcttgaaa aaattaacta
     1921 ctcaggtcgt ctgggctggt tttgctccct ctccctctgg gagagggcgg gggtgaggga
     1981 tggcggtctg tgcagggagg tatcgtgggt tgtggcttgc tggccctcac cccaaccctc
     2041 tcccggaggg cgagggggca ggaaaagcgc gctgcatggc agccccggaa agcctattgc
     2101 ggcctatcat cgccggatga acgccaccac cacccccgcc gcagtcaccg aactttccct
     2161 tcgttccctg tttgatgccc agcaccgcgc cagccgcgcc caggtggacg tgccgctggc
     2221 cctgcgccgg gacaggctgc agcgcatgcg ccgcctgctc gatgaacacg gcccggcgct
     2281 ggcgctggcc gtgcaggccg attttggcgt gcgttcgccg cagctgaccg aaattgccga
     2341 tatgtttgtg ctgcgcacct tgctgtcgca cacgctgaaa catctgccga agtgggtccg
     2401 gccggtgaag gtgtccacgc cgatctacct gcagccctcg agcgcctatc tgcagcgcca
     2461 gccgctgggc gtggtcgggg tgatctcgcc ctggaactac ccggtgcagc tggcgctggc
     2521 gccggccatc acggccctgg cggcgggcaa ccgcgtcatg ctcaagccga gcgagctgac
     2581 gccgcgcacc tcggcgctag tgggcgagct gatcggccag gcgtttgcgg ccgacgagtt
     2641 ctgcgtggtc gagggcgacg ccgcgctggc ggcggccttc acccacctgc cttttgacca
     2701 cctggtgttc accggctcca ccgccaccgg gcgcaaggtg gccgaggccg ccgccgccaa
     2761 cctgaccccc accacgctgg agctgggcgg caagtcgccg tgtatcgtcg acgcgtccag
     2821 cgacctcgac agcgtggccc tgaagatcgc gcacggcaag ttgctcaacg ccggccagac
     2881 ctgcattgcg cccgactacg tgctggtgcc gcagggcagg gaggccgcgt ttggcgaagc
     2941 ctttgctcgc gccgtggcca ggctgtttcc caccattgcc ggcaaccccg actacgcagc
     3001 catcatcagc ccgcgccacc acgagcgcct gcaggcgttg ctggcccagg ccgaaggcga
     3061 gggcgcgcgg ctgcaggtgg tgaacccggg tggcgctgcg cccgccactg ccaggcagat
     3121 ggcgccggtg ctggtgttcg gcaccacacc ggccatgaag ctgatggacg aggaaatttt
     3181 cggcccggtg ctgccggtgc tgccgtatgc cacgctggac gaagccatcg gctacatcaa
     3241 cgaccggccg cgtccgctgg ccctttactg gttcggcacc gacaccgcct cgcgtgacgc
     3301 ggtgctggcg cgcaccgtga gcggcggcgt gacggtcaac gacacactga tgcacattgc
     3361 ccacgacaac ctgccctttg gcggcgttgg cgacagcggc tggggcgcct accacggtga
     3421 aaccggcttc ctgcgtttca cgcagcaaaa accggtgttg gtgtcgtcga agtgggcgcg
     3481 cggcgacctg ttttacccgc cgtatggcaa acggttcgac cagatcatgg ggctgctcaa
     3541 acgctggttg taaggcagtc agacggtcgt tcccgcagcg gtcttttccg gcaggcaggg
     3601 aagggataat gaaacgatgt tgttgaaagc gttcatcacg accctgtggc tttgtgccgc
     3661 catgggcctg ggcggcctgc cgggccgcgc gcaggcgcag ccggtggtgg ttgcggcgtc
     3721 ggacctgaag ttcgcgctgg aggaagtggc cgctttgttc gagaaggaaa aagggaagaa
     3781 gatccgcctg gtgttcggct cgtcgggcaa cttctattcg cagctcctgc agggcgcgcc
     3841 attccagatg tttctctcgg ccgacgagga ctttgtgttc aaactggcgg acgccggcag
     3901 gacgcgcgac cgcggcaggc tgtatgcgct gggccgcatc ggcatcctgg tgccgcatgg
     3961 ttcggtcctg aaagccgaca gcgaactgaa ggacctggcg gcggcgctga aggacgggcg
     4021 gctgcagaag ttcgccatcg ccaaccccga tcacgcccct tatggcgccc gggccaggga
     4081 ggcgctgcaa catgccggcc tgtggagcca gctgcagccg cggctggtgc tcggtgaaaa
     4141 catctcccag gccgcgcagt ttgccacctc ggggggcgcc cagggcggca tcatcgcgca
     4201 gtcgctggcg ctggcgccca gcgtgggcag gctgggcacg tttgcgctga ttcccgacag
     4261 ctggcaccag ccgctgcgcc agcgcatggt gctgctgaaa aatgccgagc cggctgccca
     4321 ggcgttttac gacttcatcg gcacgccggc cgcgcaggcg gtgatgggcc gctacggctt
     4381 cgtgatgccc taagccgcca tggactggtc cgcgcttttt ctctcgctca agctggccgc
     4441 cgtcacactg gtgatcctgc tgcccgtcgg tgtgctgacc gggcgctggc tggcctacac
     4501 cggctttcgc ggcaaggcct gggtcgaggc cgtggtggtc ttgccgctgg tgctgccgcc
     4561 caccgtgctc ggttattacc tgctggtgtc gctgggcggc ggctcgccca tcgggcagtg
     4621 ggcgcggcag tggttcggcg tcgagctgac cttcaacttc ctgggcctgc tgatcgcctc
     4681 gctcgtgttc aacattccct tcatggtgca gccggtgcag cgcgccttcg aggccgtgcc
     4741 ccgcaacctg gccgaagccg ccgccgtcag cggactgggt ggctggcaga cctttcgcct
     4801 ggtggaactg ccgctggtct ggcccggcgt gttgtcggcc atggtgctga cctttgtgca
     4861 cacgctgggc gagttcggcg tggtgctgat ggtgggcggc agcattccgg gtgaaacccg
     4921 caccattgcc attgccatct acgaccaggt gcagggtttc aacctggcgg ctgccaacca
     4981 gatgtcgctg gccttgctgc tgctgtcgtt gctggcggtc ggtttttcct ttgttgcgtc
     5041 ggcccggctg gtgcgtcgtc catgagtgca ccggcaagca gggcgctggt ggtcgaggtg
     5101 gagcaggccc agcccatggc cctgtcggcg cagtttcgct gcgcgccggg cgagatgctg
     5161 gccctggtgg ggccgtcggg tgccggaaaa acatcgatgc tgcgcatcat ggccgggctc
     5221 atgcgtccgg cgcacgggcg tgtgctggtg ggtgagcagg tctggtgcga cacggccagc
     5281 ggcgtgtttg tgccgccgca aaaacgccat gtcggcctgg tgttccagaa ctacgcgctg
     5341 atgccgcacc tgagcgccct ggacaacgtg gcgctggcgc tgatgcacct gccgcgcgaa
     5401 cagcgccgcg agcgtgcgcg gcgctggctc gaccatgtgc gcctgacgcc
//
LOCUS       DAIDFG010000207         5430 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_207_length_5430_cov_19.444279, whole genome shotgun sequence.
ACCESSION   DAIDFG010000207 DAIDFG010000000
VERSION     DAIDFG010000207.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207_length_5430_cov_19.444279"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          907 a         1899 c         1836 g          788 t
ORIGIN      
        1 cgcggcggcg ggcgaaaaag cccggtgttt ggccagcagg cgcgcggttt ccagcgcctg
       61 tggaatctgc ccggcctggc gggtggcgcg caatttggtg cgcagcgcca gcgtacggcg
      121 cgacgcgccc tgcggcaact ggttgagcag cgccagcgcg ccgagcgggt cccggtcgtc
      181 gagtgcccag cgtgcggcgc gcagctgcac accttcgcgg gtctcctggg caatccggtg
      241 gctgctgact tccagcgcct gctgcaactg ctcttcacgc tcggccttgt tctgcagggc
      301 ctgcgcactt tcggcagcca gcaggtgcgc cagcagacgc agctgggtcg cgcgcgccgg
      361 cgtgtagccg ccgacatggc cggaggcgcg tgccagcagg ctgaggcttt tttcctgggt
      421 gatggcgttc tgcgcgagtt tggccgcgcg gataaagcgc ccggccatca ggtgcgacag
      481 cgcgtccatg aaggcggcgt acatggcacg ctctttttgc tgggcgcgcc agcgcctggc
      541 ctcggccggc agcgaaaaca ccgccaccac ggcacgcagc gcgaggtaca gcagggtgaa
      601 caggccggcc agcagcagca ccagcaggtt gaaggaaatg tcgacgcggt gcggcggcca
      661 gaacagcgtc accaccgcct ggttgttgcc ggccaccagc gccaccgcca ccgcaatgcc
      721 gaacagggcc aggagccaga gagcgctgcg cataccgagc ccggcttcct agcggcccgc
      781 ggccgccgtg gccagcgcgg ccagggtttc gtccaggcgc ggcaactcgc tggacttcat
      841 ctggctttgc acctgctgca gcagctgggc tgccgcctgg gtcctgcgcg acgcaggatc
      901 aaagtacttt gccagcgagg cgccggcggc ggccaggtcg gaacgggcgc tggccgtctg
      961 gcgcgacaac aggccgagcc gggcgttgag cagtttgagc ttgaggtttt cacgcaggaa
     1021 aaacgcctgc tcgggtgcca gcagcgcggc gtcgggctgg tcgatgcggc tcacgcgcag
     1081 cagaccgcgg gcttcgtcca gcgagccctg caacgtgcgc tgccaccagg ccctgagcgc
     1141 caggctgtcc agccaggcca tggaggtgcc tgctgaggag gcgggcctgg ccggcacggg
     1201 tgcagccgcc ggcgccacca aacgggcggc ggccgcgccg gtcaccatcg cgttggccag
     1261 cggcagatcg tccaccgcgc gggtcagttc atcaagtttg agcagcaggc cgggcacatc
     1321 ggtcaccgtg gtggccttga tgcgctccac gtcccgggcg atggcgcgct gcagcggatt
     1381 gaggcgcggt tgcgcggcgc gcgtgagccg ctggtcggcc gacttgagcg ccgccagcag
     1441 cggcccggcg ccgccggtca gctgggcctg ctgctgggcc agacgcagtg cagactccac
     1501 atcgaccacc aggttttcat cgcgcgagcg cgacaggctt tgcatcagtt cttccagctg
     1561 ggtgcgctgc aggctcacct cactgagccg cgtctccagc agtgccaggc gcgccgtcag
     1621 ttcgcgcgtg ccttcctgcg cctgccgcgc cagtgtgcgg gcctcgatgg actgcgcggt
     1681 cgcgtcggtg ctgcgcctgg ccagctcttc ctggatgttg ccgagctttt gccacagcag
     1741 gatgctgagc acgaggccgg caatggccac caagccggtc accagcagcc aacggcgccc
     1801 cgcacccgcc gctggtgcgg ctggcagctc ggccaggggc accggcaagg gaggggggac
     1861 gggttcgctg tcgctcatgg tggtattgat tctatcgagc ccacgatatc cgccatctcc
     1921 ggacgtgact gcacaacaac accccagccg gcggcacgcg ccgcttcggc aatgcgtggg
     1981 tgggtggcga cggcgcgggc gcgcgaccag tcccctgccg gcaggtgggc cagcgcctcg
     2041 gagctgctca gcagccagac cgagccgtcg tgcgccgcgg ctgctatgcg ttgccgctgc
     2101 gattcagtga aaaccggcgc gcggcgctgg tacaccgcca ccacctcgac ccgggcaccc
     2161 gcggcggcga actgctgcgc cagccagtcg cgcccggccc cgcccgcctg gtccttgcct
     2221 gcgccatgac cgcgcacgat cagcacgcgc tggccctgcc aatggcgctg gttcaccacc
     2281 gcccacaagg cttcggagtc gaactgcccg gcgtctgccg ccggggcgtc gatcagcgcc
     2341 tcgggtactc ccgcacggcg cagcgcggcc accgttcccg gaccgggcgc cagaaagcgc
     2401 ggccccgcgc ttgccattga tgctattgaa tcagtagctg cttgcgcctt ctgcacaagg
     2461 gccgcagcct gattggatgc aaaaaatccg tctacggcat tgccgctgac aaacatcaag
     2521 gccgaatagg taccgatctc gcgccaggcc tgctgcaggg ccggcgactc cggcagcgca
     2581 gcgatctcga tcaggggcag cgcctcggcc tgcaggccgt gcgcctgcag ggactgcacc
     2641 cagccggcgg cctcgcgctc aggccgggtg acgatgacac gcacagcgtt caaggctgcg
     2701 gcgcaggggc ggcccagacg gcgccgccgg cccgcaagga agcggccacg cgttcaccca
     2761 gggcctcggc gtccgcaaag gtatgcaccg acgcgcggtg ttcggcacgc accagcggct
     2821 ggccggccac gccttgtccg tccgcgccgg gctcgcccca ggcggcttcc agacacatct
     2881 cgtcgccctg cggttcgcgg atgaaggcgg ccagcggcat cgagcagctg ccccccatgg
     2941 cacgcgacac ggtgcgctcg gccgtcaccg ccagccaggt tggctggtgc gccagcgtgg
     3001 ccagcgcttc gagcagatcc tgccggtcgg cacgcacttc gatgcccagt gcgccctgcc
     3061 cggcggccgg cagcatctgc gccggatcga aggcacagcg gatgcgcgaa gccaggccca
     3121 ggcgtttcag gccggcggcg gccagcacga tggcctggta ttgcccttcg tcgagcttgc
     3181 gcaggcgggt gtcgaggttg ccgcgcaggg gttcaatttt caggtccggc cgcagggcct
     3241 tgagcaacac cagacggcgc aggctggagg tgccgacgac ggcgccgggc ggcagctcgt
     3301 ccagcgacga aaaatgattc gacaccagtg catcacgcgg gtcctctcgc tcgagcacgc
     3361 aggccagcgc aaagccgtct ggcaggtcca tgggcacgtc tttcagcgaa tgcacggcga
     3421 tgtcggcgcg gccgtcgagc agcgcggttt ccagctcctt gacgaacagg cccttgccgc
     3481 cgaccttgct cagcgagcgg tccaggatct ggtcacccag cgtggtcatg cccagcagct
     3541 cgacctgcca gcccaggcgg gtctgcagca aggccttgac gtgttcggcc tgccacaggg
     3601 cgagacggct ttcgcgcgtg gcaatgacaa cgcggaggga aggggaagaa gctgcgctca
     3661 ctcgtaacct gtctgtaatc ttggggggtg cccggatgct agcatgctgc actgcaaaac
     3721 aagagccaac acgaggtgaa ccatggtaac gacggcgcgt gcgacccccc ccaaggaaca
     3781 aaccggggga tctgccaaag acagtgaacg cccgcgcgcc cgaagcaggg acaacgagcg
     3841 cccgctggtc gaggacatcc gcctgctcgg ccgcatcctc ggcgacgtga tccgcgagca
     3901 ggaaggggtg gccgcctacg agctgatcga ggaagtgcgc aagctgtccg tggcctttcg
     3961 ccgcgacgcc gaccacgagg ccgacaaggc cctcaaaaag ctgctcaagg gcctgtccgg
     4021 cgaccagacc gtcagcgtga tccgcgcctt cacctacttc agccacctgg ccaacctggc
     4081 cgaggaccgg caccacatcc gccgccgcgc cattcacgaa cgtgccggcg acacccagga
     4141 aggcagcatc gaagtggcgc tggcccgcct gcgctgggcg ggaatagctc ccaaaacaat
     4201 agcaaacatg ctggcacaca gttttgtctc gccggtgctc accgcgcacc cgaccgaggt
     4261 gcagcgcaag agcatcctgg acgccgagcg cgacatcgcg cagttgctga cggcgcgcga
     4321 cgagatcaag gcgctggccc tggccgtcca tgcggcgcgc gacgcgctga cgccgcgcga
     4381 actcgccgcc aacgaagcgc agctgcgcgc gcgcgtgatg cagctgtggc agacccggct
     4441 gctgcgtttc accaaactca cggtggccga cgagatcgag aatgcgctga gttattacga
     4501 atcgaccttt ctgcgtgaaa ttcccaaaat ctacgccgac ctcgaacgtg cgctgggcaa
     4561 ccagccggtg cacagctttc tgcgcatggg ccagtggata ggcggcgacc gggacggcaa
     4621 ccccaacgtc agcgcggaca ccctcaacta cgccctgcgc cgccaggccg aagtggcgct
     4681 gcgccactac ctgaccgagg tgcattacct gggtggcgag ctctcgctgt cggccatgct
     4741 ggtggacttc acgccgcaga tgcgggcgct ggccgaaagc tcgcccgaca ccaacgaaca
     4801 ccgcctcgac gagccctacc gccgcgcgct gaccggcgtc tacgcacggc tggccgccac
     4861 actgaaagat ctcaccggca ccgaagccgc gcgccatgcg gtggcgccgc aaaacgccta
     4921 cctgacgtcc ggggactttc tgactgacct gcgcacgatt gcctcctcgc tgcaggccaa
     4981 ccacggcggc gcgctggtcg cgcagcgcct gcacccgctg atccgtgcgg tggaggtgtt
     5041 cggctttcac ctggccacgg tggacctgcg ccagagctcg gacaaacacg aggaagtggt
     5101 tgccgagctg ctggccgtcg cgcgtatcga gccccactac gccggcctcg acgaaatggc
     5161 caagcgggaa ctgttgctgc aactgctcaa cgacgccagg ccgctgcgtg tggtcggcac
     5221 cacctactcg gcgcacgcca ccggcgaact ggcgattttc gaggcggccc gcacggcgcg
     5281 tgcacgtttc ggcaaggagg ccatccgcca ctacatcatc agccacaccg aaaccgtcag
     5341 cgacctactg gaagtgctac tgctgcaaaa ggaagagggt ctgatgcaca acacgctgga
     5401 cagcgctgca agcaacgacc tgatcgtcgt
//
LOCUS       DAIDFG010000208         5410 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_208_length_5410_cov_16.420915, whole genome shotgun sequence.
ACCESSION   DAIDFG010000208 DAIDFG010000000
VERSION     DAIDFG010000208.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208_length_5410_cov_16.420915"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          909 a         1701 c         1767 g         1033 t
ORIGIN      
        1 cgagccgctg gcgcgctgtc ttgaaaaaat ccgcgaggac cgcgccgaac cggccggcca
       61 gcaggcgccg gtggtcctgt tttcgccgat cgggcgtgcg ctcgaccatg gcggcgtggc
      121 tgcgtggtcg gccagcgctg gtgcggtcct gatctgcggc cgctacgaag ggctcgacca
      181 gcgttttatc gacacctatg tggaccagca gatcagcttg ggcgactttg tgctgtccgg
      241 tggtgaaatt gccgcgatgg ccttgctgga cgcggtggcg cgcctgcaac cgggcgtgct
      301 ccatgacgag ggcagccacc agttcgacag tttcaacccg gcgctggatg gcctgctcga
      361 ctgcccgcat tacacccggc ccgagacctg gcgcggccag acggccccgg agacgctttt
      421 gtcgggcaac cacggccaca tcgagcgctg gcgccgcgag cagcgcctgg ccctgactcg
      481 gcagcaccgg ccagaactgc tggacaaggc ccgcgcagcc ggcagcctga ctgccgccga
      541 tgaggccttt ttgagcgatc tgccgtcgca aaaggccccg aagccataaa tggctataat
      601 caaaggcttt tcggtcctct ggcggccact gcaaccatct tgaccatccc ctgatgtgtc
      661 catggatttg cggtctttag cgtagcgcgt gtatgaacga aaaagttaga tggaataaat
      721 catgaatctg atccagaccc tagagcagga agaaattgcc cgcctgaaca agaccatccc
      781 cgtgtacgcc cccggcgaca ccgtgattgt cagcgtcaac gtggttgaag gtacccgcaa
      841 gcgtatgcag gcttttgaag gcgtcgtgat tgccaagcgc aaccgcggcc tgaactccag
      901 cttcatcgtg cgcaagatct ccaacggcga aggcgtcgag cggaccttcc aggtctacag
      961 cccgctgatc gccaagatcg aagtcaaacg ccgtggtgac gtgcgtcgtg ccaagctgta
     1021 ctacctgcgc agccgcagcg gcaagtcggc ccgtatcaag gaaaagctgg gcgccaaagc
     1081 tgcataaaac agccggcccg caaaaaaaag ccgctctccg gagcggcttt ttttatgccc
     1141 tgttaatctt gcgttctcat gacgacgccc cggttcacca cgcctttgcc cacctttgat
     1201 ccccgcagca tcccggtgat cggggtggac acgcacctgt cgggcgtgcc ggcacaggaa
     1261 ctgacgccgc aggccctgcg ccagcgtttc cggcacccgc cggtctggac gccggaacac
     1321 agtgttgaaa agaagttttc caaccgtgaa ccggcactgg cggcggtgct gctgccactg
     1381 gtcatgcgtg atgaactgac gctgctgctg acggagcggg ccggcaacat gtccacccat
     1441 tccggccaga ttgcgctgcc cggcggcaag accgacgaca ccgaccacga tgcggtggac
     1501 acggccctgc gcgaggcgca cgaagaaatc ggactgacgc gcgagcatgt ggaggtgctg
     1561 ggcatcctgc ccacctatgt caccggcacg gccttcatca tcacgccggt cgtcgcgctg
     1621 gtgcggctcg gttttgcgct gcagcccagc ccgtccgagg tggccgacat ttttgaagtg
     1681 ccgctgcgtt acctgatgga cccgtccaac caccagcgcc atgagtttga gttcgatggt
     1741 gtgctgcgcc agtggctgtc catgccctac atgggggatg agggcgcgga cgcgaaggag
     1801 cgctacatct ggggcgcgac ggccggcatg ctgcgcaacc tctaccgctt tctgagcgca
     1861 tagacaagcc acccccgtcc aggctcactg cgtgtagcct gttcccccct caaggggcgg
     1921 cgcctgcggt ctggcaaagc cagtcccgcg gcccctgctg gttgcgttca ctgaactctt
     1981 caacccagca tgggccgcgg gtccggctcc gccggtccgc aggcgcagcg cccctttggg
     2041 ggctgagcgc gacacgcagt gcgcgaccgt gggggcagtt atgatttgcc atgagttttt
     2101 ttgctgtttt gtttgcactc atcattgaac aggccaggcc gctggcgcgc ggcaacccgg
     2161 ttcatgccgg cttgcgccgc tgggcgcgct gggccagccg cagcctggac gccggcaagc
     2221 cgcaacacgg ctgggtcgcc tgggccacgg cggtggtggg cccggccctg ctgaccctgg
     2281 tggtgcactg gttgctctgg agcgtcaacg gtgtgctggc ttttgcctgg agcgtggccg
     2341 tgctttatgt gacgctcggc tttcgccagt tcagccacta tttcaccgac atacgcgatg
     2401 cgctggatga cggcgacgaa gcgaccgcgc gcgagctgct ggcgcagtgg cgtcaggtcg
     2461 atgcaagcga actgccgcgc agcgaaatcg tgcggcatgt gatcgagtat tcggtgctgg
     2521 ccgcgcaccg ccacgtgttt ggcgtgctgg cctggttttc ggtgctggct gcgctgggcc
     2581 tggggccggc cggtgccgtg ctgtaccgca tggccgagtt tgtctcgcgc tactgggcct
     2641 acaagtccaa ctcgaccggc gaagagacaa gcccggccct gcagcaggcc gctgcgcggg
     2701 cctggggcgt gatcgactac gtgccggcac gcctgacctc actggggttc gcggtcgtgg
     2761 gcagtttcga ggacgcgatc gacggctggc gcaactacat gcagcgtgcg cccgcggggc
     2821 cggaaaaccg caacgacggc gtgatcctgg ccgcgacctc gggagcggtc aacgtgcgcc
     2881 tgggcggcga agcgttgaaa gctgcgtttt ctcccgacgc ctcccagggc ttccaggccg
     2941 acggcatcga cgcaagcggc ccgcaggcca ccgagagtac gccggggcgt gagccggagg
     3001 tggggcacct gcgcagcatc gtgggcctgg tctggcgctc cgtggtgctc tggatgatgc
     3061 tgctggcgct gctgacgctg gcgcgcctgc tgggctgaac gctgcagcag aggttctccg
     3121 tgacttccca gacctccttc tggagcccgg ccctgcgagg gctgacccct tacacacccg
     3181 gcgagcaacc tgtcatcgaa ggcctggtca agctcaacac caacgaaaat ccgtatccac
     3241 catcgccccg ggttgttgcg gccatcaccg ccgtcgcgca ggagggactg cagctctacc
     3301 ccgatccgca ggctgccggg ctcaggcagg cgattgcgga ttttcacggc atcgctgcca
     3361 gccaggtatt tgccggcaac ggctcggacg aggtgctggc gcatgctttc ttcgcttttt
     3421 tccggcaagc ccgcccggtg ctcatgccgg acatcagcta cagcttctac aaggtttact
     3481 gcggcctgta cggtattgaa tccgccttgg tcccgctgga tgcggccatg cgcgtccagg
     3541 tggcgcatta ccggtcttcc tgcggtggcg tggtcatcgc caacccgaac gcgccaaccg
     3601 gcacaggttt gccgctggcc gaaatcgagc agctgctgca acaacaaagc cagtgtgtgg
     3661 tgctggtcga tgaagcctat gtggatttcg gcggcgaaag cgccatcggc ctgatcgccc
     3721 gctacccgaa tttgctggtg gttcaaacct tgtccaaatc acgttcgctg gcggggctgc
     3781 gtgtggggtt tgccgccggc cagcgccacc tgatcgaggc gctggagacc gtgcgggaca
     3841 gcttcaattc ctacccgctg gaccgcctcg cactggcggg tgcgatggcc gcgtacgagg
     3901 accacgcctg gtttgaaacc acgcgtgcag ccgtcatcgc gagccgcgaa gacctggcgc
     3961 gtgatctgca ggcgttgggt tttgaagtgc tgccgtcaca ggctaatttc ctgatggtcc
     4021 gccatcccgc gcatgacgcc gcggcactgg ccgcgggctt gcgcgagcag cgcatcctgg
     4081 tccgccattt caggctgccg cgtatcgagc agtacctgcg catcagtgtg gggacgcccg
     4141 cacagtgcgc agcgctggtg caggcctgcc gcgcgctggt gagcggttga accttttcag
     4201 taacgcgggg gctgcgacag ctcggcgaaa agttcgctat acaaacgata gcggctagcg
     4261 cttatttcgc ttgggccaga ggctgatttg acctctgaaa tcacgccaca accgggttcg
     4321 tgcaaatggg tgcagttgta gaacttgcag tcctgcgcat gggccttgag gtcgggcatg
     4381 tagttggcca actgcatcgg ctcgatgtgg tgcaggccga actcctgaaa gcccggcgaa
     4441 tcgatcagcg cgctggttct ggcgtcgtcg agccagtaca gcgtggtgct ggtggtcgtg
     4501 tgtttgcctg aattgagcgc ttgcgatatt tcggcggtca gcacctgggc gcccggaatc
     4561 agcaagttgg tcaggctgct cttgccggca ccggaggggc ccagcaccag cgtttttttg
     4621 ccgcgcagca gctcgcgcag ctgggcggtt tgcgcgtcgt tggcaatggg ggtgtcggtt
     4681 ttcggcttca ccgccagcgg caccacctgg taacccatgg cgcggtaggg cgccagcttg
     4741 gtccaggcgc gtgcaaaggg ttcggtcagg tcgcttttgt tgagcgcgat gatgggcgtg
     4801 atgcgctcgg cctcggccgc gatcagcgcg cgggtcagct ggctctccga gaactcgggc
     4861 tcggccgcga tcaggatcag cacctggtcg aggttggccg caaacgactt ggtgcgcatg
     4921 tcatcctgtc ggtaaaacag gttgctgcgt ttttccacct tctctatcgt gccttcgtcc
     4981 tgagagggca gccacaacac gcggtcgccg acaacggcct ggcttttctt gccgcgtgga
     5041 tggcagatca ggcgctggcc gtcgggcgtt tccaccaggc aatgccggcc gaaaccggcc
     5101 accaccaggc cgggcagggt cccggcggaa gaaacagtgg tcaagccagc agcgcgtcaa
     5161 ccttggacgc gcagaaaaag tcactgaccg aaatgccgtt cacgtcgtgt gtgttgaaac
     5221 gcaccgtgca tttgccataa ctgagcgcca ggtccgggtg atggtcctcg gcattggcaa
     5281 tgaaagccac ggcgttggca aaacccatgg tctcgtggaa gttcctgaag ctgaaggttt
     5341 tttccagcga accgtcgatc agacgccagc cctgcggttg ctcgccgttg agctggctca
     5401 gctgcgtgac
//
LOCUS       DAIDFG010000209         5408 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_209_length_5408_cov_19.188492, whole genome shotgun sequence.
ACCESSION   DAIDFG010000209 DAIDFG010000000
VERSION     DAIDFG010000209.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209_length_5408_cov_19.188492"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          796 a         1739 c         1842 g         1031 t
ORIGIN      
        1 ttacagcggg aacgatgtta gaacaatttg ggagcgcgat gccgttttgg cccgccgcac
       61 caaattggcg catgcaagca tgcttgtcat cccggactcg atccgggatc catgcgcacg
      121 ttccaccgca agcgggcgca cgaggcatgg attgcgggtc aagcccgcaa tgacaaggaa
      181 gtgggtcggg ttggctcagg caggtgagga gatctcgccc agcgactggc cgacgtcttc
      241 ttcgtcctgc cccttgaccg cgatgtcgcc gatggaaatg atgccaacca ggcgctcagc
      301 cccgtccagc accggcaggc ggcggatctg gctggacgcc atgtcggcca gcacggtgtc
      361 caggtcgtcg ctttcgcggg cggtgcggat gtcggtgctc atcaccttgg ccagcggcgt
      421 gttgccgtcc aggccttgcg ccacggcgcg taccacgatg tcacggtcgg tcaccatgcc
      481 gagcagcttg tcgccctcgc acacggggat cacgcccacg tcgagttctt ccatggcttt
      541 ggcggcctcg gtgatggtgc tgtcggaggt gagggttcgt acatcgcggg tcatcacgtc
      601 gttgacggtg gtcatgaaaa ttctccttgg gtggcgccgg cggcaccgtg tgaccagcat
      661 ggtgcgcggc gggcgccctg tgagtcagca ggcacccatg tggccgtagg acaagcgcgc
      721 ggctcaggcg ccggcaggcg tgttggcgtg gcgctcggca tagagcgcga gttccacatc
      781 gtttttggtg ccggtttttt cgaggatgcg caggcggtag gtgctgacgg tgttggacgc
      841 caggcccagc tgcgtgccga tctcgccgac ggacttgccc gccgtgagca ggcggaacac
      901 ctggtgttcg cggtgcgaca gggcttcggc gccgctgcgc ccgccgctgc cgccgacggc
      961 actggccagc gcttcggccg tgacggctga aacatacatg ccgccgttgg ctaccttgcg
     1021 caccgcggca atcatgtcgt cggggttcgc gcttttgttc aggtaaccgg ccgcgcccag
     1081 cttgatgcag cgcaccgcgt attgtttttc ggggtaggtg ctgagcatca gcaccggcag
     1141 ttgcggccag gcttttttca gggcctgcag cacgtccatg ccgtcgcggc ccggcagcgc
     1201 gatgtcgagc agcacggcag ccagcccggc gtggccgccc agcgcgtcga ccttctcgag
     1261 gatgtcgggg ccggtctcgg cttcggccac cacggtgatc tcgggggcgt cggccagcac
     1321 ctgtttcaga ccttcgcgca cgatgcggtg gtcgtcgccg atcaggatgt tcaatggcgt
     1381 catgatgcta tggttttggg agctggatgc gcaggtggaa caaggacccg aggccgattt
     1441 ggcttgaaat ctccaggctg ccgccaagat ggcgggcgcg ttcgcgcatg cccatcacgc
     1501 cgtaagcatt ggccgcttca aaggctgcgg cggctgcgcc cttgccgttg tcctgcacgc
     1561 ggatgctgat ctcgctggcc gtggccagga gggtgatggc gatgtggctg gcctgcgcgt
     1621 ggcggccgac gttgctgagc atttcctgga agatgcggaa aatcgccatg gccgaaggct
     1681 cgggcagcgg cacggcgcgt tccacgtcca tctgccagtc gagttcgagt tcagccgact
     1741 gcacgaactc gtgcgcctgc cagtccagcg cggcccacag gccctggtgg tccaggatgc
     1801 tggggcgcag gtcggtgatg atgcggccca cgttgtccac cgcggcctcg atcagccggc
     1861 ccatgttctg gcacttgctg cgcatggtgt cgcgcatggc ctgagccgcc acgggttccc
     1921 gctgctgctg ctcgcccagg cgtttgtcca gccagccgac gtccatcttc agcgccacca
     1981 gcaggctgcc gagctcgtcg tgcacctcgc gcgcgatgcg ggtgcgctct tcctcgcgca
     2041 cggtgtgggt gtaggccgag agttcgcgca gttgctgcag cgcctcggac agcgcctggg
     2101 tacgctcact gacgcgctgc tccagcacgt cgttggcgtg gcgcaaggct tcctcggccc
     2161 gcttgcgtgc cgagatgtcg acaaaggtga ccaccgcgcc ctgcaccacg ccgccatcga
     2221 cgatggggta ggacgagtat tcaaccgcaa acgcgctgcc gtcgcggcgc cagaacagct
     2281 cggtgtcgat gcggcagggc agcgccatgc ggaaggcgtt gaagatgggg cacaggtgct
     2341 cggggtagtg cgcgccgtcg gcatggctgt ggtgcgccag ctcgtgcatg tttttaccga
     2401 ggacttcggc gggcgcaaag ccaagctgct cggcgccggc gcggttgatg aaggtgcagc
     2461 agccgtccat gtcgatgccg aaaatgcctt cgccggtgga ttccagcaac tgcgaaagct
     2521 tgtcgcgcca gaccggctgg tcggcgccag gcaggtgcat gggtgcggac agcggggcgg
     2581 tttctgcaaa catggcagct tatgagcaaa catcgtgccc agcttggtgc gcgatgcttc
     2641 gcagggtcgg gcgacccagg cgccaaatcg gtgcgttacc ggcttgcagc gccggattgc
     2701 cccaaaagag gttgttgtgg cgaatccctg taaagtcaga gctccccttc cagaaaagac
     2761 catggcgccg gtttcccctt ccctgacccc tttgatgacc tctcctttga tggtccgcgt
     2821 gacggcggcc agtctgttgc tgcttctgct cacggcgtgt aatccggttt tcaactggcg
     2881 cgaagtgcgg ccggacaaca ccgcgctgac cctgctgctg ccctgcaaac ccgacaaggc
     2941 ccagcgcagc gtgccgctgg gcgggcggcc gaccgagctg gccatgctgg gctgcgatgc
     3001 cggcgacgcc acgtttgccg tggcggtggc cacgctggac gacgcggcac aggcggcggc
     3061 cgtgctggcc ggctggcaaa ccgccacgct ggccaacctc aaggccgccg gcggcgggca
     3121 aaccagcccg ctgaagctgc ccggcgcgga tccgctgccg ccagccgtgt tggtgcgggc
     3181 cagcggccag cgcgccaacg gccgggcggt gcaaagccag gcggcgtatt tttcacaagg
     3241 ccggcaggtt tttcaggcgg tgatctacgc cgacaagatg gaccccgagg tggccgaaac
     3301 ctttttctcc agcctccagc tggacgccgg aaccccccgc tgatgggcgc cgttcgggac
     3361 gccgggcgcc tgccggccgc agctgccgcg tctggcctcc tgagccggcg cgccgccatc
     3421 gtggtgtttc tggcgtttgc agcggcctac ttctgttcga cgctggtgcg ggccatcacg
     3481 gccacgctgt cgccggtgct cacgcaggag ttttccttgc atgcgcgcga cctggggctg
     3541 ctggccggcg gttacttcct ggggtttgca gtgacccagc tgccgctagg cacctggctg
     3601 gaccggcatg ggcccaaacg tgtgatcctg tattttctgg ggctggcggt gctggcctgc
     3661 ctggcgtttt caatggcgac cagtttcagc gggctgctgc tggcgcggat cggcatgggc
     3721 atgggggtca gcgcctgcct gatggcgccg ctgaccggtt accgccgctg gtttgacgcc
     3781 ggcaccctgc tgcgcgccaa ctcctggatg ctgatgaccg gctcgctcgg catgttggcg
     3841 tccaccctgc cggtgcagtg gctgatgccg ctgaccggct ggcggccgct gttctggatt
     3901 ctggcggcgc tgatcttgtt gtccatggtg gcgattgcct gggtggtccc gtcatggaag
     3961 gtcgcctccg cggtggcttc tccctctgtg tcgcatcagc aggccagtta cgcccaggtc
     4021 tggcgcagcc gctatttcca gaaaatgtcg ccggtggcct tttttcacta tggcggtgtg
     4081 attgccatgc agaccctgtg ggccgggccg tggatggtcc gggtggccgg ctacacgccg
     4141 ctgcaggccg ccaccgggct gttctggatc aacgcctgca tgctggtcac cttctgggcc
     4201 tgggggctgg tcaacccgtg gctcgcgcag catggctgga gcgccaaccg cctgatcacc
     4261 tggggtgtgc cggtcagcct ggtggtgctc gccgtcaata tcgcggccgg accggccacc
     4321 ggctgggtgg gctgggcgct gttttgcatg agttccagcg tgctgggcct ggcccagccg
     4381 gccgtgggca tggcgtttcc gcaggcgctg gccggacgcg cgctgaccgc ctacaacctc
     4441 gtgatttttg ccggggtgtt tgtcatgcag tggggcatcg gcctgctgat tgacgttttt
     4501 caggccatgg ggctggccga cgtggccagc tttcaggcgg cgctggccgt gtttttgtgt
     4561 tgttgtctcg catcgtatgc cttcttccac ggcgccaaag cagaccccac ccccgataat
     4621 tcaccgcaat gaacggcatt ctcatcatcg cccacgctcc gctggcttcg gccctgcggc
     4681 aatgtgttct gcatgttttt cccgacaacc cggccagtgt ggccgcactg gacgtgcagc
     4741 ccaacatgcc gcccgaagaa accctggcgc aggcgcgcat tgtgttcaag cagatgggca
     4801 cctcgcacgc gctggtgctg gccgacgtgt ttggcgccac accctgcaat gtggcgcaga
     4861 agctggtcga tggtgttcac tccaagctca tcaccggcgt caacctgccc atgctgctgc
     4921 gcaccgtgtc ctaccgccac gagccgctgg acgcgctggt ggcccgggcg gtgatgggcg
     4981 gaacgcaggg cgtgatgcag gtggccatca ccgcaccgca gaaccaggta cgcagaaacc
     5041 atgatcaaga ccagcacgac catcaacaat aaactgggcc tgcacgcccg cgcctcggcc
     5101 aagctgacca agctcgccgg cagctttccc tgcgagatct ggatcgccaa aggcgagcgc
     5161 cgcgtcaacg ccaaaagcat catgggcgtg atgatgctgg ccgccggcat cggcagcacc
     5221 gtcgatatcg aaaccgatgg ggctgacgag caacaggcga tggaagcgct gctggccctg
     5281 attcacgaca agttcggcga aggagagtga ctagtgcccc cacggtcgct cacttcgtgt
     5341 agctccctgc cccccgaggg ggccgccccg cctgcggccc ggcaaagccg gttccgcggc
     5401 tcggactg
//
LOCUS       DAIDFG010000210         5396 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_210_length_5396_cov_17.946826, whole genome shotgun sequence.
ACCESSION   DAIDFG010000210 DAIDFG010000000
VERSION     DAIDFG010000210.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5396)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210_length_5396_cov_17.946826"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          834 a         1694 c         1881 g          987 t
ORIGIN      
        1 gccactggcc agcggacgtt ccggacagct ctggtgcgtt tttggctgcg gcggtgagcg
       61 tgatgccggc aagcggccgc tgatggcggc agcggcggaa aaagggtcgg acagggtggt
      121 ggtgaccagt gacaacccgc gcagcgaaaa ccccgacgcc atcatcagcc agatcctgca
      181 gggatttgcc cgccgcgatg cggtgcaggt tcaggcagac cgcgcccggg cgatcgccca
      241 tgcggtgggc caggcgcagg ccggcgacgt ggtcctgatt gccggcaagg gccatgaaag
      301 ttaccaggaa accgcgggta tcaagctgcc gttctccgac aaggcgcagg ccgaagcggc
      361 gctggatcag ggtgcgagcc gccatgccgc gccgggaggc ctcgcatgat gaccctgcaa
      421 caggccctgc agtggttgcc gtctgcccgg ctggtgggcg acggcaccct gcggttccag
      481 cgcgtgcaca cggacacgcg cagtctgcag cccggcgacc tgtttgtggc gctccagggt
      541 gaacgctttg atgctcatga ttttctggcg caggcgaaaa gcgctggcgc ggtggcggcc
      601 ctggcgcagc atggcctgag tgccgaactc cccggcctgc tggtggccga ttcgaagcag
      661 gcgctgggcc agcttgccgc aggctggcgc gcgcagttca gcctgccgct gatcgccgtg
      721 accggcagca atggcaagac cacggtcacg caaatggtgg cgtccatcct gcgcgcgtgg
      781 aagcagggcg gtgcttttgc gaccgaagga aacttcaaca acgacatcgg cgtgcccttg
      841 acgctgctgc gtctgcgtac cacccaccag gccggcgtgg tcgaactggg catgaaccac
      901 cccggtgaaa tagccagcct cgccgccatg acgcggccca ccgtggcgtt ggtcaacaat
      961 gcgcagcgcg aacacctgga gttcatggct acggtggaag ccgtcgcccg cgaaaacggc
     1021 gcggtcatcg cggcgctgcc ggcctcgggc gtcgcggtgt ttccggccga cgagctgtac
     1081 acaccactgt ggcgggaact cgccgggtcc cgcgcggtgc tgacctttgc cgagtcgggc
     1141 gaggccgacg tcaccggcac cgcgcaatgg ctgggcggcg cctggcgggt gttggccggc
     1201 acgccgcagg gcgatctcga cttttcgctg cagatcgccg gccggcacaa tgtcaaaaat
     1261 gccctggccg ccaccgcctg tgccctggcg gccggcgcgc cgctggcggc catttctgcc
     1321 gggctgtcgg cgtttgtccc ggtcaagggc cgctcgcgcg ccctccagct ccaggcggcg
     1381 ggccacgccc tcacgctgat cgacgacagc tacaacgcca acccggattc cgtgcgtgcg
     1441 gccatcgacg tgctggccga actgcccggg ccgcgcctgc tggtgctggg cgacatgggc
     1501 gaggtcggcg atcaggggcc ggctttccat gtcgaggtcg gtgcccatgc gcgtgagcgc
     1561 ggcatcgagc aggtgttcac gctgggcgcg cagtccgccg acgctgccag cgccttcggc
     1621 ggcggccggc atttcggcga cgacatcgag gccctgaagg ccgccgtgat ggctgccttg
     1681 ccgggcgtgt ccagcgtgct ggtcaagggt tcacgcttca tgaagatgga gcgcgtggtc
     1741 caggccatcg cggccgacgc acaacaacaa aaagaggaga agccccatgc tgcttagcct
     1801 ggcccagtgg ctgcagacca tctcgccgga atttggctac ttccgcgtgt tccagtacct
     1861 gacgtttcgc gcggtgatgg ccgccatgac cgccttgctg atcggtttga ttgccggccc
     1921 ttatgtgatt cgccgtctga tcagcctcaa gatcggtcag cctatccgcg aatatgcgat
     1981 gcagacccac ctgagcaaga gcggcacgcc gaccatgggc ggcgtcctga tcctgctggc
     2041 catcggcatt tccacgctgc tgtggtttga cctgagcaac cgttttgtct ggattgtgct
     2101 gatcgtcacg ctcgggtttg gcgccatcgg ctgggccgac gactggcgca aggtggtgca
     2161 caaggatccc gaaggcatgc gctcgcggga aaaatacttc tggcaatcgg tgatcggcct
     2221 gctcgccgcg ttgtacctgg tattcagcat ttcggaaagc tccaacctgc gggtgctgga
     2281 gctgttcatg agctgggtgc agtccggttt tgacgtgaac ctgccgccca aggccgggct
     2341 gcagctgcct ttcttcaagg aaatcagtta cccgctcggc gtgtttggtt ttgtgctgct
     2401 cacctacctc gtgattgtcg gttccagcaa tgccgtgaac ctgaccgacg ggctggacgg
     2461 tctggcgatc atgccggtcg tgatggttgg ctcggcgctc ggcgtgtttg cctatgtaac
     2521 gggcagtgcc gtctattcga agtacctgtt tttcccgcac atcccgggtg cgggcgagtt
     2581 gctgattttc tgctcggcca tggccggtgc cggcctcgcc ttcctgtggt tcaacaccca
     2641 tcccgcgcag gtcttcatgg gcgacgtggg cgcgctggcg ctgggcgccg cgctgggaac
     2701 gattgcggtc atcgtgcgcc aggaaatcgt gctggccatc atgggcggca tctttgttgt
     2761 cgaagccatg tcagtgatgc tgcaggtgat ctatttcaaa tacacccgca ggcgttttgg
     2821 tgaaggccgg cggctgttcc ggatggcgcc cctgcaccat catttcgaga aaagcggctg
     2881 gaaggaaacg caggtggtcg tgcgtttctg gatcatcacc atgctgctgt gcctggttgg
     2941 cctgtcgacg ttgaaactgc gatgaatacc aacgaatccc ccaaccccgg caaagcgcca
     3001 gatgggcccg aagagcctcc agtggaggtg acgcaggcgc acggactggt gcctgcgtcg
     3061 gcgccggatg ccatgcccct ggtggagtcg gcatcaccag ccccggtggc ggaagcggac
     3121 ttggcccgca ccgggggggc gggcaaagcg ggggcacggc ccctgcccga gaccctgacg
     3181 gccgcgcgtg atgccgctgc ctttgtcgcg caattgtttg ccgaggtgag tgcgccggaa
     3241 caggccacgc cgatcccctc tgtggtgcaa gtgccatcgt cgacggacga aaccgtgctg
     3301 ggtgatgctg aaaacgaggc gcctgctgcc gtcgagcccg cgggcgagac ggtggtgtcg
     3361 gcgctgcacg gccagcatgt cctcatcctg gggctgggcg cttccgggct ggccatggcc
     3421 cgctggtgtg tgcgtcaggg cgcgcgtgtg acggtggccg atacccgcga gacaccacct
     3481 caactggcga gcttgcaagc ggagttgccg caggtcgatt tccgcgccgg cgcattcgag
     3541 tccggtctgg tcgagggcaa ggacatccgc gccgtcttca agagcccggg tctgtcgcct
     3601 gcggtgtttg ccccggtctg gcaggcggca caggccatcg gcttgtggac aggcggggaa
     3661 ctggacctgt tcgcccgcgc attgcgtgag ctgcaggccg aacgcgctta tgccccccag
     3721 gtactggcga tcaccgggac caacggcaag acgaccacga cggcgctcac tggccagttg
     3781 gtggcccacg gcggcaagac cgtggcagtg gcgggcaaca tcggccccaa cctgctggac
     3841 atgttggccg ggcacctcga tgcgggaacc ttgcccgagg tctgggtgct ggagctgtcg
     3901 agtttccagc tcgacggcgt gcagggcttc gagcccaccg cagccgcggt gttgaacctg
     3961 acgcaggacc atctggactg gcacggcagc atgcaagcct acggcgcggc caaggcacgc
     4021 atcttcggca gccagggcct gctggtgctc aatcgggatg atccggcggt gatggccatg
     4081 ctgccggagc cggtgcgcct gaagctgcaa aagccgaagt ggcgcgagta tgtgagtttt
     4141 gggctggagg ccccgcagcg accgggtgac tatggcattg aaaccgtcaa tggcatggcc
     4201 tggctggtgc gtgctgctga ggccgacgaa accgtcaagc gccgcaagga cgaagaagtg
     4261 gtgctgtaca tccagcgcct gatgccggtc gaagccctgc gcatccgtgg ccgccacaac
     4321 gccaccaacg cgctggccgc gctggcgctg gccggcgccg tgggcggcaa catggcgtcc
     4381 atgctgcacg gcctgcgtga atacaccggt gagccgcatc gcgtgacgcc ggtggcggtg
     4441 gtgcgcgacg tcgaatactt tgatgacagc aagggcacca acgtgggtgc caccgtggcg
     4501 gcattgaccg gcctgggcgc cgaacgcaag ctggtgctga tcgccggggg cgaaggcaag
     4561 gggcaggact tttctccatt ggccggaccc gtgcgccagc atgccagggc ggtcgtgctg
     4621 atcgggcgcg atgcgcccct gatccgtgct gcactggaag gcacgggcgt gcccctgctg
     4681 gatgcggatt ccatgcaggg cgccgtagaa ctggcggccc ggcaggcgca aaccggcgat
     4741 gcggtgttga tgtcgccggc ctgcgcgagt tttgacatgt tcgacaacta tgggcatcgc
     4801 gcccaggtgt tttgcgacgc cgtccaggcg ctcgcgcagg atgcgggggt ggtgtcatga
     4861 cggtggctgc ccgtctttcc ggctggttct ccagtctggg cggggccgcg cccgagggcc
     4921 tgccggtccg gctgggcggc cagcgccact cgcgttccga gtcggcgccg gtccgcatgt
     4981 cgggcgtgga ccaggcactg atctgggtca cggtggcgct gctggtctgg ggcctggtca
     5041 tggtgtactc cgcctccatc gccatgccgg acaacccgaa gttctcgcgt tatgcacaca
     5101 cccacttcct gctgcgccat gcgctgtcgc tgggggtggc ctttgcggtt gccctgctga
     5161 ctttccaggt gccggtggcg acctgggaaa aaatcgcacc ctggctgttt gtggcctcgc
     5221 tggtgctgct gatgctggtg ctgatcccct tcatcggcaa gggcgtcaat ggcgcgcgcc
     5281 gctggatctc gctgggtttc atgaacttcc agccctcgga actcgcgaag tttgcggtgc
     5341 tgctgtacgc cgccgactac atggtgcgca agatggaggt caaggagcgc tttttc
//
LOCUS       DAIDFG010000211         5378 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_211_length_5378_cov_17.891415, whole genome shotgun sequence.
ACCESSION   DAIDFG010000211 DAIDFG010000000
VERSION     DAIDFG010000211.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_211_length_5378_cov_17.891415"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          958 a         1818 c         1728 g          874 t
ORIGIN      
        1 caaggtcatg accatgctgc tgggcgcggt ggcggccgtg agcctgctgg tgggcggcat
       61 cggcatcatg aacatcatgc tggtcagcgt gaccgagcgc acccgcgaga ttggcctgcg
      121 gctggcgatc ggcgcgctgg agcgcgaggt gctgctgcag tttttgatag aggcggtggt
      181 gctggcagcg ctgggcggcc tgatcggcat cgtgctggcc accggcgcct ccttggcgct
      241 ggcggccgtg atggatgtgc cctacctctt caacccggct gtgaacctgc tgtccttcct
      301 gttctcggcc ggcatagggg tgctgttcgg ctattttccg gcccggcgcg cggcgcgcat
      361 ggaccccatc gacgcgttgc ggcacgaata aaacccggtg gccctcatgc cgtggtcaaa
      421 aaactctggt tgcgtttggc gagcatgctg ttccccatgc ccgcctgctc tgcacaacgc
      481 agcaataagc caaaaggcca gccaggcgtc ggtgggcatc accccctgga gcctgcgctg
      541 aagcgcacgg gtgacggcca gacccgctca tgccacagga accgtcctgc catctggacg
      601 gcctttctgc agggacccgg aaagcctgaa aaccccgggc aacgaccgcc tggtcttgac
      661 aagggtcaag tgcactttgc ccgaaaaggt gccgaatggg agcctggctt cagccggtct
      721 gttgggtcca accatcagcc ccggcaggat ccggcgccgc aggtgcaaga aagaagaact
      781 ttcagccaga cgcatctacg gcatccctgc cgcaggggtt gcggcatttt tttgacagat
      841 ggagatctcc atgaaaccac tttcatgctc aatggcggta ctgctgatgg ccgcttcggt
      901 tgcaggaccc gtgctggcca aaggtccatg ggccgccaac ccggggaaca cctggggtta
      961 ccaactcatg accccgcaag agcggaccga gcaccaagcc aggatgcgca gcttcaagag
     1021 ctacgaagag tgcaaggcct accaggaaga aaaccacaag cagatggaag cacgcgccag
     1081 ggaaaaaggc acccccctcc cggccatgcg ggcgggcgga ttcggctgcg acaacatgaa
     1141 ggcgcgtggc tacctgaagt aaacccgccg aaggctggca gacagtgccg acttggcagg
     1201 catgaactcc gccgggtccg gcggagcatg cttcaacccg gctgcagccc cttggcgctg
     1261 accgcctggt ccccctgccg ggaatccgcc aattcggcgc gcatcaacgg caggtgctgc
     1321 ggatactcgc gctgcatgaa atcgaccagc ccctcgcgca catggcagcg caggtcccag
     1381 ttccggccgg agctggccga ggtcacgagc aggcgcagct gcagggcacc ctcgcccgct
     1441 tccgtgacct gcagcttgca caggcgccgg tcccaatcgg gtgacgcttc gcacagacgc
     1501 tgcgcttcgg cgcgcagggg ctccatcggc atgcggtagt ccacccacag gaacaccgtg
     1561 ccgatgatgc tggcgctttt gcgggtccag ttctggaagg ggtgctcaat aaaccattgc
     1621 agcggaatga tgagccggcg ctcgtcccag atcttgagca ccacataggt cccggtgatt
     1681 tcctccaccc ggccccactc gccttccacg atcaggacgt cgtcaagccg gatgggttgc
     1741 gccagcgcaa tctgcagccc ggcaatcagg ttgctgaaaa tcggccgggc ggcaatcccc
     1801 accaccaggc ccaccacgcc ggccgacgcc agcagactgg taccaaactg ccgcgccccc
     1861 gggaaggtca tcagcatcag ggccagcccg gtcagcacga cggtgaacat cgcggttcgc
     1921 gccagaaggc gggtctgggt gtgaatgcgg cgcgcgtgca ggttgtcttc gacatcaacc
     1981 gggtgcaggt cggtgacgcc gtgcgccaca ccgcgaacgg cgcgcagccc cagccaggtc
     2041 aatgccgtga acagcaacag agcattggcg tggcgcacgc cgcctatcag cgccaggtcg
     2101 tcgggtgcgc cctgccaaac ggcctgcagg gcaatcagcg gcagcagcag ttgggccggc
     2161 gccctgcaat gccggaccac cgtcgcagcg accggcatgg aacgtgtcag ccgcagcagc
     2221 acggccaggc ccacacgatg caccagcagg gccagcgcca cagcaatacc cgccaccaac
     2281 agaagccgca gcgacgaatc ttctgcccag gccagcattt ccatcagttc cgggtatttc
     2341 atgaaagacg ccttcaggcc acgcgcggtt tgacccgcga ggcagccagc ttggcctggg
     2401 cccgcgcagt ctccacatcg tcatcgaagg ccagcgcgac gcccatgcgg cgcttgacaa
     2461 aactctccgg cttgccgaac agccggatgt cggtgttggg tacgcgcagt gcctcggcca
     2521 cgccgtcaaa cacgatgccc tgcgcatcca caccgccgta gatcacggca ctggcgcccg
     2581 gggttttgag cgccgtattg accggcaggc caagaatggc tcgcgcatgc agctcgaact
     2641 cgttttgcca ctgtgtacac atcgtcacca tgccggtgtc gtgcggccgg gggctgacct
     2701 cgctgaacca gacctcgtcg cccttgacga acagttcgac gccaaacagg cccagaccgc
     2761 ccggcttgcc gtcatggccc aaccccaggt tgtcggtcac cgccttgcag atgtcgcggc
     2821 ttttctgcag cgctctggag gccatgggct gcggctgcca gctttccaca tagtccccgc
     2881 tgacctgcac atggccgatc ggctcgcaga aatgggtttc gatctgccca ttcgcgccca
     2941 tggcacgcac cgtaagctgg gtgatctcgt agtcaaagtc gatgaagccc tcgacgatga
     3001 cccggccgtg gctgacgcgg ccgccggcca tggcatggtc ccaggcggcc ttgacgtcgg
     3061 cgggaccgga aatcttgctc tggcccttgc ccgaggaact catgaccggc ttcacgacgc
     3121 aggggtagcc gattttggcg tcgatcgcgg cctgaagttc cgccagcgaa tcgcaaaata
     3181 cataggggct ggtcggcagg cccagggttt cggcggccag gcggcgaatg ccttcgcggt
     3241 ccatggtcag ccgggcagcc cgcgcggtcg gcaccacacg gaccacgccc gccgcttcga
     3301 gttcttccag catcggagtg gcgattgcct cgatctcggg caccaccaag tgcggccttt
     3361 ccttttcaat cagcgccttg agctgggccg ggtcgctcat ggtgatggtg cgtgcatggt
     3421 gcgcgacctg ctggcccgga gcgttgtcgt agcgatcagt ggcgatggtt tccacgccca
     3481 gacgctgcag cgcaatcagc acttccttgc caagctcgcc ggagccgagc agcatgactt
     3541 tggttgcgtg gggggacagg ggggtgccga aggtggtcat gggatgtgca ggaggtggag
     3601 gggtcggagg acaggtcgcc atgcaaacga gccgtaagcg aggcgccgtg accaacactt
     3661 tacttcaccg gggaaaacac ctgggcccgg ggcccggtcc ccttcagcgg atggccctac
     3721 ggaacacggt cgcgcggatt ctggatgaca atggttctca ttggcgcccg cctgcttcag
     3781 aaagcccttc ttgacccagc cccttccccc tcccgcacgc ccgcaaccag cgtccagccc
     3841 ggcgcccgtc cgccagcgcc gtccgccgcg ccgcgtggaa gtcagccgca tcgaggtcct
     3901 gagcccggcc atgcgccgca tcacgctcca gggtgccgaa ctcgagggct tcgcgccggc
     3961 cgggcccgcc agctatatca agctgatctt ccctgagccg gggcaaaccg agccgacccg
     4021 ctctttgccg gacggcccgc gcgccgtgtc catgcgcacc tacaccccgc tggcggtgcg
     4081 gcccgaggcg caggaagtcg atgtcgattt tgtgctgcac ggtgaaggcc cggcgtcgac
     4141 ctgggcggcc caggccaccg tcggccaggt gctgttcctg atgggccccg gtcccggcta
     4201 tgccttggac agcgccgcca cgcagcacct gctgatcgcc gatgccagcg cgctgccagc
     4261 ggtcgaaacc attctggccg ccctgcccgc cggcgcccat gcgcaggtgc tgctggaagt
     4321 catcagcgat accgagcagc ggccgctgca cagcgcggcg cagcttgacg tggtctggct
     4381 ggcgcgcggc acggaccacc gggcggcggg acaggccctg gaaaaggcgc tgcatgctgc
     4441 tgcgccgata ggccccgaca cccgtattta cctggcctgt gaggccgcgg cgatgcgtcg
     4501 catccgccag ttgctgatca cggcgctggg cgtgagccgc agccagatcg tgggccgggg
     4561 ttactggaag ctggacgtgg tcaaccaccc ggatcacgac tatggtgagg acgcctgaaa
     4621 cccgtgtccg ttcagccgct gtcccgtacc gcggcagcag cccagcgcag gtcggcaaca
     4681 atagccgcat gaaccatccc cttctccaga tccggggcct gaccaaacac tacggcagcg
     4741 ctgtggtcgt ggacgacctg tccttcgaga ttgcgcccgg cgaatgcctg ggcgtgatcg
     4801 gccctaacgg cgccggaaaa accaccacca tccgcatgtg cctgggcctg accgcaccgg
     4861 acaccggcga catcacttat tttgcggacg gccagaaccg cggtttgcgc atgcccgacg
     4921 atgcgctggc gatcaaggcc aggctgggcg tggtcagcca gttcgacacg ctggacccgg
     4981 acttcagctg cgcggaaaac ctgcaggtct atggccgcta tttcggcatg aaggcggcgc
     5041 aggtgcgcgc ccgcgtgccg gcgctgctgg aatttgcctc gctctcgcac aaggccggca
     5101 gcaagcccgg tgaactgtcg ggcggcatga agcggcgcct gagtctggcg cgcgccctcg
     5161 tcaacgaccc gagcctgctg ctgctcgacg aacccacgac cgggctggac ccgcaggcgc
     5221 ggcacctgat gtgggagcgg ctgcaactgc tgctgcaaca gggcaagtcg atcctgctga
     5281 ccacccactt catggacgag gccgaacgcc tgtgctcgcg cctgctggtg ctggaccacg
     5341 gcaagaagat cgccgaaggc aaaccgcgcg agctgatt
//
LOCUS       DAIDFG010000212         5351 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_212_length_5351_cov_16.162009, whole genome shotgun sequence.
ACCESSION   DAIDFG010000212 DAIDFG010000000
VERSION     DAIDFG010000212.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5351)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5351
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212_length_5351_cov_16.162009"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          915 a         1686 c         1795 g          955 t
ORIGIN      
        1 caagcaggcc gcccacgagc gcctgcgcct gtcggacctg accttgccgc atgccatggt
       61 gcgtgtgctg ctcatcgagc agctctaccg cgcctggagc atcacggtca accaccccta
      121 tcacagggaa taatgggccc ccacgttcgt tcactgcgtg taactctctg ccccccgggg
      181 gggcgctgcg cctgcgggcc ggcggagccg gacccgcggc ccccgctggg ctgaatcggg
      241 gatgtggctt gcctcaggac ttcaaacatg aacgacttca tttaccttgc gtctcaaagc
      301 ccgcgccgcc gccagctgct ggagcagctg ggcgtgtcct gcgaactgct gctgcctgac
      361 gccggcgaag acagcgaggc gctcgaggtg gtgctggctg gcgaggcgcc gaccgcttat
      421 gtccagcgcg tcacgcagct caagcttgac gccgccgtgg cgcgcctgaa acagcgcggt
      481 ttgccgccgg caccgatttt gtgttccgac accacggtgg cgatggggcg ccgcatctat
      541 ggcaaacctg acgatgccgc ccatgccgaa agcatgctgg ctgaactggc cggacgaacc
      601 caccgggtgc tgacggcggt ggcggtgcag cagggccgca agcgggttca ggcgctaagc
      661 gtgtcacggg tgacttttac gcccatgagc cgggcccaga tccgcgccta cgtcgccacc
      721 ggcgagccgc tgggcaaggc cggtgcctac gccatccagg gcagggtggc gatgttcatc
      781 tcgcacatga gcggcagtta ttccggcatc atgggcctgc cgctgcatga aaccgcgctg
      841 ctgctgcaag ccgcaggcct gaaaatataa aaagcgcccc acaagaagac accatgcaac
      901 aagacatcct gatcaactgg tcgccccagg aaacccgtgt ggccatggtc gagtacgggg
      961 cggtgcagga gctgcatgtc gagcgcacgc tggagcgcgg cctggtcggc aatgtctacc
     1021 tcggcaaggt ggtccgggtg ctgccgggca tgcagtcggc cttcatcgac atcggcctgg
     1081 aacgcgccgc gtttttgcat gtggcggatt tgatgagcag catcaacagc cggcatgccg
     1141 atgccgacaa cgcggctacc cccgcggcag cactgcagcc aattgaaaaa cagctgtttg
     1201 taggccagtc gctgatggtg caggtgctca aggaaccgat tggcaccaag ggcgcgaggc
     1261 tgaccgcgca ggtcagcatc gccggccggc tgctggtgtt tttgccgcag gacaaccaca
     1321 tcggcgtgtc gcaaaaaatt cccgccgcac agcgcgagga cttgcgccag cgcctgcagg
     1381 ccctgaccgg cgacatgggc ggcggcttca tcctgcgcac caatggcgag gacgccaccg
     1441 atgcggagct cggtgaggat atcgcctacc tgcgcaagac ctgggcccgc atcaaggacg
     1501 ccgcgctacg tttgccgcca acctcggtgc tgcaccagga tctgaacctg ctgcagcgcg
     1561 tgctgcgcga catggtggtg gaaaccacgc agagcatacg catcgactca cgcgagcagt
     1621 tcgacaaact caggcagttc gccacggagt tcatgccggc gaccctgcag cggctgcagc
     1681 tgcacagcgg cgagcgcccg atttttgatc tcttcaacat cgacgaggaa attgccaagg
     1741 cgctgggccg gcgggtggac ctgaagtcgg gcggctacct gatcatcgac cagaccgagg
     1801 cgctgaccac ggtggacgtc aacaccggcg gttttgtcgg tgcgcgcaat tttgacgaca
     1861 cgattttcaa gaccaacctg gaagcctcgc aggcgattgc gcggcagctg cgcctgcgca
     1921 acctgggcgg catcgtgatc gtcgacttca tcgacatgct gcgtgacagc caccgcgacg
     1981 ccgtgctggc ggagttccag aagcagctcg cgcgcgaccg catcaagacc agcgtcaacg
     2041 gcttttcggc gctgggcctg ctcgagatga cgcgcaagcg cacgcgcgag tcgctggcgc
     2101 accagctgtg cgaaccgtgc agcgcctgca tgggcaaggg cgtggtcaag acggcgcgca
     2161 gcgtcggtta cgacatcctg cgcgagatcc tgcgcgaggc gcgccagttc aatccccggg
     2221 agttcagggt gattgcctcg cccaaggtga tcgagctgtt tctcgacgag gaaagccagc
     2281 acctggcggg cctgagcgac ttcatcggca agccgatctc gctgcagtcc gaagccgcca
     2341 tggcgcagga acagtacgat atcgtcctgc tctgaagcac tgcctcctgt cctgcatgcc
     2401 cgccacgccc gccagccccc agcgcccgat accgattctg gtctaccacc agatcagcga
     2461 agcgccgccc aaaggtgcgc ctttccgcag cctctacgtt tcgccggtct ccttttcgcg
     2521 ccagatggcc ctgctgaagc tgctgggcta ccaggggttg tcgatgagcg cgctgctgcc
     2581 ctatctgcgc ggtgaaaaaa ccggcaaggt ggtgggcatc acctttgacg atggttaccg
     2641 caacaacctg acccacgcgc tgccggtgct gcagcgtcag gggttttcat cgacctgtta
     2701 tgccgtcagc gggctgctgg gcaagaccaa cgagtgggac cagggcatag gcatggcgca
     2761 ggtgccgctg atgacggcgg acgagttgcg cctgtgggtg gccgggggcc aggaggtcgg
     2821 atcacacacg caaaaccacg cgcgtctgct gcaaagcgac gaccccaccg cgctcgatga
     2881 aatgaccggg gacagggcag cactcgaagg gctgctggcc acctccgtgc ggcatttttg
     2941 ttatcccttc ggtgaatacg cgccgcagca cgtggccatg gctcgccagg ccgggttcca
     3001 gaccgtcact acgacgcagc gtggccgcag ctccgcccgg aacgacctga tggagttgcc
     3061 gcgggtgccc gtcgtgcgtt ccaccagtct ggcggtgttc tggctcaagg tggcgacggc
     3121 ctacgaagac cgggaccgca ggtgagcggc tcatccgttc agcctgaggc gccacgcagg
     3181 ctgcgtattg cggtgctgaa ccgtgttttt gacccggccg ggggtggggc ggagcggtat
     3241 tcgattgcgc tggtggaaca gctcgcgcag cgccacgaga tccatgtgtt tgcgcagcag
     3301 atcaggcacc acgcgccggg cgtgatttac caccccgttt ccatgccgct gcgcaagccg
     3361 cgctgggtga accagctctg gtacgcgacc gccacctggt gggccacgcg ccggggtttc
     3421 gacattgttc actcccatga aaacacctgg cagggccagg tgcagaccgt gcatgtgctg
     3481 ccggtgaaat acaacctgtt tcatggccgg accggctggc ggcgcgtgct ggcttgggcc
     3541 aaggtctgga gcagcccgcg cctgttgacc tacctggggc tggagcgcct gcgtgtcgcg
     3601 gcccggggcg ggcgctgcgt ggtggtgacg tcggccagcc tgggcgacac tctggccgcg
     3661 acctaccccg gcacgctggc cgtcaccacc gtgatcacac cgggggtggt gttgcccgcg
     3721 gcacccgccg gtgcgccgga acaaggtgcg gcccgcatgc agctgggcct gccgcccaag
     3781 ggacgctgtc tgctgcttgt gggaaatgac taccgcaaaa agggcctggg gacgctgctg
     3841 cgggtgctgc gcgacctgcc ggcggacgtg gtgctggcgg tggtgggcaa cccggggcag
     3901 atcccggtgt ttgaacggca ggcgcaggcg gagggcgtgg ccgggcgcgt gtttttcctc
     3961 ggttcgctgc aggacgtggc gcctgcgtac cgcgccgcgg actgcctggt gcacccgacg
     4021 ctggaggata cgtttgccat ggtggtcctg gaggccatgg cgcacggcct gcccgtggtg
     4081 gtcagcagtg cggcgtattg cggtatttca agcctgctca gccatggcgc cgatgcgctg
     4141 ctgctggaca atccgcagga tgctgcgaaa ctggccggca ccctccagca agtgctcggc
     4201 gacgaggtgc tgcgacagca gttgggtagc caggcgaggg cgtttgcaag ccgctaccag
     4261 tggccggcga ttgcgcgcca gcaggaagcg gtgtatttcg ccacgctggc cgcggagcag
     4321 cgctgacaaa ctgcgttcag gccctcaggc caccagcttg aagtgttttc ggctaaagtc
     4381 ccgcccggtg agcaactcga ttctgcgcat caggtggtat ttgttttcct tgcgggtcgg
     4441 ctggtaagcc gggtcgatct gcaggtcctg ctcggcgccc gtgagcagcc attcctgcac
     4501 gactttcggg tgggttccct tgaaagcttc cagggcgcgc gcatcgaact ggctgtactt
     4561 gatctgcatg gtcgttgagt cggcccagta cttgctgacc tggtcgagct ttttctgcat
     4621 gtcctcattg cggcgaatcc agccgtagtg gtagatgtgg gcgttggcca gtgccgcctg
     4681 cgggttgcgc ggtcgcttgt gctcggtggt caccagccag tactggccgt cgggcgcgta
     4741 ggagcggatg gtgttcctga tcagccggca ctcgcggcgg taccagccgg ggctgaccga
     4801 gacccactgt gcggagccgt aaaagtggtg gtaatcaaag accagcgctt ccaccgccgg
     4861 gttgccatgg tgcttctcga cgctggcgcg gatcgcggcc aggtcctcct cgtgaaccac
     4921 ctcgtcgccc tcgaggtaga aggcccagtc gccggtgcag gaatactgcg caatcatctt
     4981 ctgttgcgcg taaacaaagc cgcggtcggc catgcgttcg ttccagacgg tttcgatcac
     5041 gcggattttc gggtcgccaa tcgccagcac gcgcgccagc gtttcgtcgt tgccgcggcc
     5101 caccgcgacg acgaattcgt cgaccagcgg caacagggag cggatcgagg cttcaaacgg
     5161 gaagcccagc tcgacgccgt tgcggatgaa ggtgaaacca ctgatggaaa ttgtcatgaa
     5221 agttgcgcca cggggggact ttgcgataga tcggcgtcgt acagcccgag cttgtagagg
     5281 cgggaatagg ggccgtcgcg ttcaatgagt tcggcgtgtg tgccggtttc gatgattcgg
     5341 ccggcatcca t
//
LOCUS       DAIDFG010000213         5331 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_213_length_5331_cov_17.803639, whole genome shotgun sequence.
ACCESSION   DAIDFG010000213 DAIDFG010000000
VERSION     DAIDFG010000213.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213_length_5331_cov_17.803639"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          995 a         1762 c         1611 g          963 t
ORIGIN      
        1 gcgcggtcgc gctcgtgata aatggccggt gtcaagtgac atgacggata gtacaggctg
       61 cttgtgttcg gtgcaggaca tacacgtctt cagcgtttca gccgggcgac cagccccggt
      121 gccgacgtca tggcaacgat gatctggaac acttcctgct tgctgcagtg caacattcat
      181 cccttgatgc actgaccaga aggagcccgc catgctggac cgcaccacaa ccaccttctc
      241 ccacgtcaag cccggcgata ccgagtttgt ctcgggcggt ttgcgcgatt ttttcctcta
      301 ccgcgacctc ggcattgccg aggcaaccca cggcaaggtg attgcgcacc tggtcaaggc
      361 gaacatggca ccagaaacag gcacgggctg gcaccgccat gaggcggact tccagattgt
      421 catcatggtt aaaggctggg cgcgcttcat gtacgaggac aaggaaaccc tggtcgaagc
      481 cggcgacgtg gtgcaccagc gcccgggcat acgccactat ctcttcgact attcgcccga
      541 catggaatac ctggagatcg tctcgccagc agacttcaag tcgatcgatg tcgaacccgt
      601 ttgcgacatc ccgccgccca ccccctggtg aatagagccc ccacggtcgc tcacttcgag
      661 cctgcggcgg ccctgcccct tgcaccgaaa aaggggccgc tgcgcctgca ggccggcaaa
      721 accggacctg cggccccggt ttgacaaaga aatctcgacg ccggcacttg tggtgctttc
      781 cagcaggggc cgcggaaccg gcttcgccgg gccgcaggcg ccgccccctg caagggggga
      841 acaggctaca cgcagtgagc ctggacgggg gtgttcagaa ctcagcgtcc agctcgtcaa
      901 tgtcgtcggg ctccttcttc gcggcggcca cccactcctt catggcgggc agctccatga
      961 tgcgtttgca gtaggccatg cacaccttgt ccagcgccac gtcgtaggtc aaaaagcgcg
     1021 taacgaccgg cgcgtacatg gcgtcggcca cgccgggttg cttgccgaac aggtagggcc
     1081 cgccgtaggt ggccaaacac tccttccaga tgtccagcac gcggtcgatg tcggcctgcg
     1141 cccgcgacca gaccttgaag ttggggaagt gcgctttgag gttcatgggc aacgaggaac
     1201 gcatcgatga aaagcccgaa tgcatctcgc cgcagatggc gcggcagtgc gcgcgcgcct
     1261 cgacgtcggc cggaagcaga tgcgcacccg gcttgatttc gtggaggtat tcggcaatcg
     1321 ccagcgtgtc ccagaccttg acgccgttgt gctgcagcga aggcaccaac atggacggcg
     1381 ccagcagcag catttcggcc ttcatggccg ggtcgtcggg ggaaatcacc ttttcggtga
     1441 agtccagccc cgcgagtttg gccagcagcc agccgcgcag ggaccaggcg ccgtagttct
     1501 tgctgctgat cgtgagaacg gctttggcca tgggatgctc ctggaaagtg cgtgggtact
     1561 gcagcgcgga agtacctgtg caagggctgt gccagaaaag tgcatggcgc atacgccggt
     1621 tggcccgcaa cttgcctgca tgcctgcatc gggccaccag ccccgcttca agccaggacg
     1681 cccatgcttt accaagccta ccaaacccag tccgacctga tgtcgccgtt tcgcctgctg
     1741 gcccagggca gcagcgccgc cctctggctg ggcaaaaccg agggcagctt gctgcgcaag
     1801 ttgtcagcct cgatggaggt gttctcgcgc ctgcgggtca cccattcgcg cccggcctat
     1861 ggcatcagca cggtgaaagt gggcgaacgc gaactcgcgg tgaccgagga aaccgtgctc
     1921 accatgccct tcggcaccct gctgcgattc aaaaaggaag aagcgaccga ccggccgcac
     1981 cagccgaccg tgctgctggt ggcgccgctc tcgggccact ttgccaccct gctgcgcgag
     2041 accgtgcgca cactgctgca ggaccacgat gtctacatca ccgactggca caacgcgcgc
     2101 gacgtggcgc tgcgccacgg cccgttcggg ctggacgact acatagccca catgatccgc
     2161 ttcactcagg ccatcggccc cggcacccat gtggtcgcgg tgtgccagcc ctgcgtggcc
     2221 gcgctggcgg ccgcggccat catggccgag gacgacgacc cggcccagcc gcgcagcctg
     2281 acgctgatgg cgggcccggt cgattgccgc gtcaacccga ccgccgtcaa caccctggcc
     2341 accagcaaac ccatcgagtg gttcgagaaa aacctgatcg gccatgtgcc gctgccgcac
     2401 gccggctaca tgcggcgcgt ctaccccggc tttgtacagc tcagcgcctt cctgagcatg
     2461 aacctggacc gccacaagaa gtccttcaag gacatgtacc agcacctggt cgacggcgac
     2521 gtggacaagg ccaacgtgat ccgcgtgttc tacgacgagt acctggccgt caacgacctg
     2581 ccggccgagt tctacctgga aacggtcgag aaagtgttcc agacctacga cctggccgtc
     2641 ggcaagctca gctaccgcgg ccgcatcgtc aacccggcgg ccatccgccg caccgcgctg
     2701 atgacggtcg agggcgaacg cgacgacatc tgctcggtcg gccagacggt ggccgcgcag
     2761 gacctgtgca gcagcatccg tcccttcatg aaaacccacc atgtgcagac cggcgtgggg
     2821 cactacggcg tgttcagcgg caggcgctgg aaccagcaga tttatccgcg ggtgcgggag
     2881 atgattcacg ccagcatggg ctgagtttag gaagaaataa gcctccagcc ctttaaaatc
     2941 gggcgctaac cgctattgaa tttgtagcag aatcttctga tgcatgtcat cccggacttg
     3001 atccgggatt catgtttgct gactaccggt ttttgtttgg gactttgtgg ttagccaaca
     3061 agccgggcaa cccccggctt gctggcacac cactgccggt agaaaagaaa cagtgttcgc
     3121 gaattcctct gcggacggag gctctgcgga gcctcagagc ttggtctctg gcgtctcgtc
     3181 ttcttccgcc ggccagtcgc ggatataagc cttgagcatc ttgttttcga agttctggcc
     3241 atcgaccaca gccttggcca cgtcgtaaaa cgagatcaca ccttcgagta cctttttgtt
     3301 gagcaccggc atgtagcgcg catggcgacc cagcatcatg cggcggactt cgtcgatctc
     3361 ggtgtcgggg gtgcaggtca gcggcgcatc gtccatggcg gtgcgaacca gcgtggcgcc
     3421 gatctcgccg ccgtttttga caatgcacag aatcacttcg cggaaggtca gcatgcccac
     3481 caggtcgccg tgctccatga cgacgaggga tccgatgtct ttttccgcca tgatctgggt
     3541 cgccttgaac agcggctcat cgggctggac ggtgtaaagc gtgttgcctt tgacgcgcag
     3601 gatatcaagg actttcatgg ggcactcctt caggaatttg tctcggcagt ctgcaggttc
     3661 ttccccggtg cagcccggcg gaaaattcag ttcaaatata gcccacaatc cgtttgcaag
     3721 ccacctcaag ttgtttcggg ctggctgccg ttttcgccta tcccttccgc cattttctgg
     3781 agacaccatg tctggctact ctgacccggg cttcgacacg ctggccctgc atgccggcgc
     3841 cgcgcccgac ccggccaccg gcgcgcgcgc cgtgccgatt cacctgacca cctcgtttgt
     3901 cttcgaatcc agcgaccatg ctgccagcct gttcaacctg gagcgcgccg gccacgtcta
     3961 ttcgcgcatc agcaacccga ccaacgcggt gctggagcag cgcgtcgccg cgctggaagg
     4021 cggcataggc gccatcagca ccgccagcgg ccaggcggcg ctgcacctga gcattgccac
     4081 gctgatgggc gcgggctccc acatcgtcgc ctccaccgcg ctgtacggcg gcagccagaa
     4141 tctgctgcac tacacgatgc ggcgtttcgg catcgagacc accttcgtca aacccggtga
     4201 catggatggc tggcgcgctg ccgtgcggcc caacaccaag ctcttttttg gcgaaaccgt
     4261 gggcaacccg gggctggacg tgctggacat gcccaccgtg tcagccatcg cgcacgaggc
     4321 cggtgtgccg ctgctggtgg actcgaccct gacatcgccc tggctgatca agccgttcga
     4381 gcacggcgcc gacctggtct accactcggc caccaagttc ctgagcggcc acggcacggt
     4441 gattggcggc attgtggtcg acggcggcag cttcgactgg gaaaagtccg gcaaattcgc
     4501 cgagctgacc gaggcttacg acggctttca caacatggtg ttcagcgagg aaagcacggt
     4561 cggcgcgttt ttgctgcgcg cgcgccgcga aggcctgcgc gacttcggcg cctgcatgag
     4621 cccgcacacc gcgtggctga tcctgcaggg catcgaaacc ctgccgctgc gcatggcgcg
     4681 gcacatgagc aacaccgaaa aggtcgtggc cttcctggcc agccacccgc tggtggcacg
     4741 cgtcggccac ccgatgctgg aatcgcaccc cagccatgcg ctggccaaaa agctgctgcc
     4801 gcgcggcgcc ggctcggtgt tcagcttcga catcaagggc aaccgtgagc agggcaaggc
     4861 cttcatcgaa accctcaagg tgttcagcca tctggccaac gtcggcgact gccgcagcct
     4921 ggtgattcac ccggccagca ccacgcattt ccgcatgacc gacgaagcgc tggcgggcgc
     4981 cggcatcacg cagggcacca tccgcctgtc catcggcctg gaagatccgg atgatctgat
     5041 cgatgacttg aagcgcgcac tcaaggccgc ggagaaagca gcatgatcct caacgtcaac
     5101 ggccacaaca cctattgcta caccggcggc aagcccttcg atgcggccaa acccaccgtg
     5161 gtcttcatcc acggcgtgct caacgaccac agtgtctgga ttttgcaaag ccgctacctg
     5221 gcccaccacg gctggaacgt gctggcggtg gacctgccgg gccactgcaa aagcgcgggc
     5281 gaggcgccat cgacggtcga agaggccgcc ggttttgtgg ccgccctgct c
//
LOCUS       DAIDFG010000214         5327 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_214_length_5327_cov_17.863809, whole genome shotgun sequence.
ACCESSION   DAIDFG010000214 DAIDFG010000000
VERSION     DAIDFG010000214.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_214_length_5327_cov_17.863809"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          854 a         1696 c         1786 g          991 t
ORIGIN      
        1 aaaagccagg acctggagca ttcggcccag ctggcgcgca ccggctggga aaaagccaaa
       61 gcggccattt tgagcggcga caacttcatg gtggtgctcg acgagctgac ctacccgctg
      121 atctacggct ggatgccgct ggccgatgtg ctgcagaccc tgaaggacag gccgaaagac
      181 gtgcatgtgg tcatcactgg ccggcgctgc ccgcccgaaa tcatcgaact ggccgacacc
      241 gtgaccgaga tgagcaaggt caaacacgcc ttcaacgctg gcataccagc gcagcgcggc
      301 atcgaagact gaagatgctg ctggcctggg acagttttca ggcggtgcag agcctggccg
      361 cgctggcagc ctgcgtggca tgggtgctgg ccttcgtgct ggtattgctg ctggccctgt
      421 ggattgacgc gcgctggggc gagccgcgcg ccgcctggca cccggtggtg gcgatggggc
      481 gttacctcga ggcttgcgga cctgtcgttt cttccttgcc gccagcgccg gcctttgtgt
      541 ccggtgccct ggcctggtgg ctgggcgtgg gggtggtggg cgcactggcc tgggggctgc
      601 aggcgctggc cctcgcgcag ctcgcccgcc tcgtcgcagc gcccggtccg gaaacagcgc
      661 tagccgcgtt gctggtggtg gtactgatgg gctgggtgct caagaccctg ctgtcctggc
      721 gcatgctgca cgacgaggtc ggcgcggtcg aggccgcgct gcagcaatcg ctggcggccg
      781 ggcgcgagcg cctgtcctgg ctggtcagcc gcgacaccgc gctgctcagc gaaagcgagg
      841 tgcgggaaag cgcgatcgag tcactcgccg aaaacctcaa cgactcggtg gtggcgccga
      901 ttttctggtt tgtgctgttc ggcctgcccg gcgccgccat ctaccgtttc gccaacaccg
      961 ccgatgcgat gtggggttac cgcggcatac atcgcggccg gcactgggag tgggccggca
     1021 agtgggcggc gcgtgccgac gacgtcttgt cctggctgcc ggcgcgcctg acggcctggc
     1081 tgctggccgg ggtggcgcgt ggcctgccga ccggtgcgtt gcgccgggaa gctgcgaaga
     1141 cgccttcgcc caacagcggc tggccgatgg cggccatggc tctggccctc ggggtcagcc
     1201 tgcgcaaacc cggcatctac atactcaacg cggcggggcg gccaccgctg cccgaagaca
     1261 ccgccctggc ccagcgttac gcatcaaaaa cggtgtttgc ccttttgctg tctgcgaaga
     1321 cagctatctt tctgatagcg atcggagtgg catggacgct gaccccatga gtcacgtcgc
     1381 gccggttttc acgcgggtgc acggcggccc cgacgctgcc ggtgtgccgc tgtttgattt
     1441 ttcgaccaac agccacgcct gcggcccgtg cccagcggcg ctggcggcgg tgcagcaggc
     1501 cgacgcgagc cgctaccccg atgccggata cgtcgccttg cgccggcagc tcgctgcctt
     1561 ccacggggtt gacgtgcagc gtgtgctgct ggcggccagc gccagcgagt tcatctttcg
     1621 catcaccgcg ctggcggtgc agcgcggcgc caaggcggtc tggttgccgc cacacagtta
     1681 tggcgactac gcgcaggctg cgcaggcgtg gaagctgtcg gtggccgcgc atgcgcaaca
     1741 cgcgcaactg cgctgggcct gcgacccctc cagcccgctg ggtgcggcgc atccagccct
     1801 tgatcaactg gccgatccgt cggcattgtg tgtgctggac cgcgcctacg aaccgctgcg
     1861 gcttgaaggc accctggcgc tgtccgacgc ggcactgcaa aatttctggc aactctggac
     1921 accgaacaag gcgctgggcc tgaccggcgt gcgtgccgcc tatgtgatcg cgccacccca
     1981 tgccggcgat gttttggtgc aactcgacca gctggcgccg tcatggccgg tcggtgcgca
     2041 tggcgtggcc atgctgcagg cctggaccac gcccgaggtg caagcctggc tggccgccag
     2101 cctggccacg ttgcgcgaat ggaaagcccg gcagatcgcg ctgtgcgaag tcatgggatg
     2161 gacttgtctg cccagcgccg cgaatttttt ctgcgcgcgg ccgctgctac cgcacggcat
     2221 gcctctgcag cagctgctgg ccgaactgcg ggggcagggc atcaagctgc gcgacaccac
     2281 gtccttcggt ttgccggggc aggtacgcat gggcgtgttg gcgccggcgg cacaggacgc
     2341 attgcacaat gcgtggcaac acatcatcag gaaagcaaca tgacggccag aagcgtgatg
     2401 gttctgggta ccaccagcgg tgccggcaaa agctggctga ccaccgcgct gtgccgctac
     2461 tacgccaggc agggcctgaa ggtcgcgccc ttcaaggcgc agaacatgag caacaacgcc
     2521 agggtagtgg cccccacgct ccgcactggc gtgtcgtcgc tgccccccga gggggctaat
     2581 tttccttggg gcgccccggc ggaaaattgg tctgccggtg gcgagatcgg cagcgcgcaa
     2641 tattttcaag cgctggcggc aaaagcggtc cctgaagtcc gcatgaaccc gctgctgctc
     2701 aagcccgaaa aagacacgca cagccaggtg atcctgatgg gccaggtcaa cgccgaactg
     2761 tcgcgcatgg cctggcgcgg ccgcagcgcc tcggtctggc cggtcatcgc gcaggccctc
     2821 gacgagctgc tggaagaaaa cgacgtcgtc gtgattgaag gcgcgggttc gccggccgag
     2881 atcaacctca aggacagcga catcgtcaac atgcgggtcg cgcaacacgc caatgcggcc
     2941 tgcctgctgg tcaccgacat cgaccgcggc ggcgcctttg cgcacctcta cggcacctgg
     3001 gccatgctcg acgaagcgga gcgccggctg atcagggggt tcgtgctcaa caagttccgc
     3061 ggcgacgcca gcctgctcgc ccccggcccg cagatgctgc aggacctgac cggcgtgccc
     3121 acggtggcga ccttgccgat gtggtggcaa cacggcctgc ccgaggaaga cggtgtcttc
     3181 gacgacagaa gccaggccac cggccacgtg acccgaacgg tggcggtgat tgcctacccg
     3241 cgcatcagca acctcgacga attccagccg ctcaagaatg tgccgggcat ccgcctgaag
     3301 tgggtgcgca gtccgggcga gctggcgggt cttgcggcct ccgactggat catcctgccc
     3361 ggctccaaac acacgagtgg cgacctggcc tggctgcgcg atcgggggct ggacagtgcg
     3421 attgccgcgc acgccgggcg cggcggggcg gtgctcggca tttgcggcgg actgcagatg
     3481 ctgggcgaag cgctgatcga tccgcacggc atcgacggca acggacccgg cctgggactg
     3541 ctgccggtgg tcacgctgtt cgaagccgac aagaccgtgc agcaccggcg ggcgcgtttt
     3601 gcgtcggttg ccgggccctg ggcggccttg tcgggtgtgg aggtgcaggg ctacgaaatc
     3661 catcacggac agaccgcgcc gcacagcgcc atggccgcgg ctggcgatgt ggcgcacgcc
     3721 gtgctgcccg ctgggctggc ctggcaaaac ggcagcggcc atgtactggg cgtctacctg
     3781 cacggactgt ttgaagaccc ggccgtgttg caagcgctgt tcggggccag cacgccgacg
     3841 ctggagtcgg tgtttgaggg gctggccgac tacatcgggc ggaatttcga cgcgggggtg
     3901 ctggacgcgc tgatcgcgcc cgggcgcgcc gcctgacagg ccgctgcgcg gcttatagtt
     3961 ggaccagctg cggtgcaccg ccttccccca cttttcggcg gggtgatggc gcccgctttt
     4021 tcccaacaag cgtttgaagg gtaaccgtgc tcactgtggt cctggccaat attgccgtcg
     4081 tgtccatcgc cgtgatcatt cactacgaat gcctgctgcg gctgaccttg ctgctcgccc
     4141 gggtgaagat ccggcatcgc ctgcgcatcc tgctgggcgt cggcggtgcg ctgctggccc
     4201 acgcggtgga ggtctgggtg tttgccttcg cctactattt cattcatcac gccgagacat
     4261 ggggcggcct gacgggaaac ttcaatggca gcctgatgga ctgcgtgtat ttttccttca
     4321 cgaccttcac cacgctgggt ttcggcgaca tccagcccac cggtgacctg cgcttcctga
     4381 cgggcatcga gtccttgacc gggctggtgc tgatcacctg gacggcgtcc tttttgtttg
     4441 tcgagatgca gaagttctgg aacgggaagt agttcccgaa ggtgcgcctg cggggcaaag
     4501 ggttgtcttc ctggcgcact gcctctggcg aaaggctttt tcccgagctt tgcaaccaag
     4561 gcgacatttg ttatcgtcct tttttcgggg agggaattta tgtttgaaaa agttgcgggt
     4621 tttgcctgtt ttatggcggt cgccatcgca ggggcactcc ccgtttccgc atttgcgcag
     4681 cctgccgaat ggttcagaac caaggtggcc gattatccca atctggtgtt tcccgcagcg
     4741 gccactgaat ggagcgtccc cagcgctacc ggaaacaaca ttttcaagcc tgcggggccc
     4801 ggtacgtttc ctgcggttgt cgttgcgcat acctgcggcg ggattgaacc ccatatctat
     4861 gaacgcgcca aggaaattct tgacgctggt tatatcgtcc tggtgctgga ctcttacacc
     4921 tcaagaaacc atttcacttt ctgcaagcca gcgggcgtga atgccacgcg ggtctacaag
     4981 gacaccttcg acgcattggc gcatctcaaa ggcatgcctg aggttgaccc gtccagggtt
     5041 tacctggtgg gattttccct cgggagtttt gccgccgccg ttgccggcag ccccgaggtg
     5101 gcttccatgc ttcaggtgaa acaccgcttc aatgcttccg tgggctggta cggcgcctgc
     5161 actttcaagc ccagcgcgtt ccatcccgcc tggaatctat tgcggaccga taccgacaag
     5221 ccggtgctgc tgctcatggc ggcgggtgac agcgaaacgc cgatcaaaga ctgcttcccc
     5281 agactggatg aaatgaaggc agcgggcaaa cctgtttcct ggcacgt
//
LOCUS       DAIDFG010000215         5314 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_215_length_5314_cov_19.842746, whole genome shotgun sequence.
ACCESSION   DAIDFG010000215 DAIDFG010000000
VERSION     DAIDFG010000215.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_215_length_5314_cov_19.842746"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          900 a         1718 c         1800 g          896 t
ORIGIN      
        1 ttttggcgac gcaaaagaaa agtgagttgc cgccgggcaa cccccggctt gctggaagca
       61 cctcaatcgc aagtactggc aatggcgttg atacgcgctg ccgcaagacc cgcatgagcc
      121 accaaaaacc accatgcaac tagaccaagc ccaacccttc ccctacaact tcgagctgaa
      181 gtccaccgcc ctcgtcatca tcgacatgca gcgcgacttc atcgagcccg gtggttttgg
      241 cgagacgctg ggcaacgatg tctctctgct gcaggctatc gtgccggctt gtcaggcggt
      301 gctcgaagcc tggcgcggcg ccggcggcct cgtggtgcac acccgggaag cgcaccgggc
      361 cgacctggcc gattgcccgc ctgccaagcg caaccggggc aatccttcgc tgcgcatcgg
      421 cgacatgggc gcgatgggcc gcatcctgat cgccggcgag cccggcaacc agatcattgc
      481 cgcgctggca ccgctgccgg gcgagatcgt catcgacaag ccgggcaagg gcgcgttcta
      541 cgccacaccg ctgcacgagc tgctgcagca ggaaggcatc acgcacctgc tgttcatggg
      601 ggtcaccacc gaggtgtgtg tgcagacctc catgcgcgaa gccaacgacc ggggctacga
      661 cagcctgctg ctggaagact gcaccgagag ttacttcccg gcgttcaagg cggcggcggt
      721 ggagatgatg cgggcgcagg gtgccatcgt cggctggacc gctgccagcg accagctgct
      781 gcctttgctg caagactgct agagttccgc catgcacacc gctgcccgtc ctttgcctgc
      841 ccttgccgag gcccccgccc accgctcgcg cgccgacgag gtgtacgaac agctcaagcg
      901 cgatgtgtcc gaattcaagc tggtgccggg cgaccgcttc accgaaaccg aaatcagcga
      961 acgtctgggc gtgtcgcgca cgccggtgcg ccaggcgctg tttcggctgc agcatgaagg
     1021 ttttgtcgag gtgctgtttc gcagcggctg gcgcgtgttg ccctttgact tcaaccagtt
     1081 cgaagaactg tatgacctgc gcatggtgct ggaaaccact gccgcgcatc gcctgtgtga
     1141 agatgccctg aaggtcaacc cggcgctgct ggacgatctg gtcgcgattt ggctggtcag
     1201 ccccgagcag cgcagcagcg acaccgtgca ggttgcgcaa tgggacgagg cctttcactg
     1261 cgcgctggtg gcggcggccg gcaatgcaga gatggcccgt gtgcaccgcg acgtgaccga
     1321 acgcatacgc gtgatccgcc ggctcgactt caccaagcag ccgcgcatcg acgccaccta
     1381 cgaggaacac gccaagatcc tcaaggcgat ccagcgcagg cgcggcgacc aggcagccct
     1441 gctgctgcgc gcccacatcc aggccagcca ggccgaggtg cgcaagatca cgctgcatca
     1501 ggtgcacctg gcacgccagc tgggccaggc ggcgcggtag gcatattcgc ccgaaaacca
     1561 tgagagaaaa tcgactcaag ccctgatggg gcgtgcgtta tcagctcatg tattcatagc
     1621 atccagagac ggcggcatgc cgtcttctga caacggctcc agggcgcggc acgacgtcac
     1681 ggcgccaccg ccgccaagcc gagcagccgg agtacgcccg acacccgctg ctcatcggac
     1741 atggcggggt ggtccgccgc ttaaccgtga ggtgttcaga tcagcaccat ggtgaagtcg
     1801 gccttgccga cattgcattc cggacaggcc cagcctgcgg gcaccgcggc ccagggcgtt
     1861 cccggggcga tgccgtcttc gggccggccg gccgcttcgt catagataaa accgcagacg
     1921 acgcacatgt agacgttcat gaattcctca ctgaagatga tgggagccgg acgccggttt
     1981 gtcagtcgtc atcgtgtgcc cggctgcctg ccgggttggt ggaaatcagg ccgttcggtg
     2041 attgctgcca ctcccacgca ccgaaggcca gcacggcgag cagcagcaag gcggccagcc
     2101 agacgcggtt tttctgcacc agatccggcc ccttgccgtc gatccggccg gtcagcatgg
     2161 gcagggcctg gtttttgcgc cgcagcaggc tcaggcctgc gatcagcgcc aggtgggcca
     2221 gaacgacgaa cagaaacgct tctccgaaaa actcgtgaac ctcttccagc cagtcgccgc
     2281 cgagaaagtc accccagtcg ttgtaggtgg cataaccgct gagcgtcagc ggcaccacca
     2341 tcagcagcag ggccaccacg gccagcgcca tcagcaggtt ctggccctgg cgccaattga
     2401 cgtcggccag cgaccggctt tgcgtgagcg agcgcagcca ggccggcgcg ccggccagct
     2461 tgcgccacat caggcccagg cccgcctggc gcggacccag caggccataa agcacacgaa
     2521 acaccagcag gccggccatg gtgtagccga gcgtgacatg cagcagccgc cagctttcgc
     2581 cgtcagcggt cacgtaggcg cccagaaagc tcagcgcaaa cagccagtgg aacatgcgcg
     2641 tgggcgcgtc ggtcacccgg cggcttggcg cggtagcggc gtgcggggcc ggtgcggggg
     2701 tgttgatgga ggggttggca tgcatggtgg ttctcctagt cgttccaggc ccggcggtaa
     2761 cgggcgtcga gcccggccgg gaagcggatg ttgtcgtcgt tgtagtcgcc cttgtcggcc
     2821 gcggtgtggc aggccgcgca gttggctgcg cttttgacac tggcgtgttt ccagacggcg
     2881 gcgtcgacct tgcggtgttt gtgttcaaac caggccgagc gggtaatgcg gtcttgcggc
     2941 ggctcttccg ccacgcgttt gtaggtgccc gcgttgacct tcagccagct gctcagctgt
     3001 tgcaccgtgg ccgcgtccag cgaggcgtcc gtgccgtagt gcttgtccag gccggtcatg
     3061 atgcgttccc aggagcgggc aggaagcatg cccggtggat aggcggtatg gcaggcggcg
     3121 cactcctggg tgtaggccgc aggcacgttg cccggcagca agcggctgct gtcggcccgg
     3181 accagggcgg gcagcgcagt gatgcccagg gtggcggcga tcaacaggga gcggacggcg
     3241 gggtgggtgt tcatgaagaa actccggtgg gtgaacggca tggctcagct cagggcttga
     3301 ggctgttgag ataggccagc acgtcggcct tttcgccagc gttgcattcg cggctcagca
     3361 catccttgca gttgcggcgg aaccacttgt ccaccttggc ggtgtcggtg aaggctttgg
     3421 ggttgaacgc cggggccagc ggtgttatcg acttgccggt actggcatgt ttgccctgcg
     3481 ccgtaggtgg cgtgccgtgg catgaggcgc aggaccattc gccgccgtgt ttggtggtaa
     3541 aaaatacccg gcctttgtcg gcattgcccg gggtcccggc ctggacgctc cagtggcgga
     3601 gctgctctgc gggcgaggtg tcggccgcct ggctggtggc gggcagcagt gcaccggcca
     3661 gcgcggcaaa tagggcggct gcgggaaaga agctgcggat caaggtcatg ctgggctttc
     3721 tgcgtttcgg gtgggatgga ctcttgcagg agatgggagg tagttttggc gaaggcgctt
     3781 gaacccttcc tgaaggcgtc gttcatctgc ggttcacaat gtgtgcagga caatcacgcc
     3841 atgacctccg tccagccttc cctctttacc ggcaaacgcc gccgttcgcg gccgggcggg
     3901 ttgtgggtgc tggcgctgct gattggcagc ctggggctgc agccggcctg gtcgggcagc
     3961 aaggatgacc acaaccgtgc gcgccaggcg gttcaggccg gccaggtgtt gccgctgcgc
     4021 agcgtgctgg agcggctgga gcgcgagcac ccgggccagg tcatggaagt cgaactggag
     4081 caggaggaag gccgctggat ctacgaagtc aagttgctgc agccgcaggg cgagctggtc
     4141 aagctcaagc tggatgcccg gaccgccgtc ctgttgcccg gcaagggcaa cagcaaaagc
     4201 gacgagcgcc gcaacggccg tgacgcacgt ccctgacacc ccgctgccag cccatgcgca
     4261 tcctcctggt tgaagacgaa cccacgctgc gtgcccagct gcgtgccggc ctggtggaag
     4321 ccggctatgc ggtggacgag gccgacaatg gccgcgatgc ccacttcctg ggagacaccg
     4381 aagccttcga tgccgtggtg ctcgacctgg gcctgccggt gctcgatggc ctgaccgtgc
     4441 tcaagcgctg gcgcgacggc ggacgggcca tgccggtgct gatcctgacg gcgcgcgaca
     4501 actggaacga aaaggtggcc ggcatcgacg ccggggccga tgactacctc accaaacctt
     4561 ttcacatgga agagctgctg gcacgtgtac gcgcgctgat ccgccgcgcc agcggcctgg
     4621 cctctgcggt gctgcagtgc ggcgcgctgg cgctggacac acgcagcggc cgcgtcacgc
     4681 tggccggcca gcccgtgagc ctgaccagcc atgagtacaa ggtgctcgat tacctgatgc
     4741 accgtccggg cgccgtggtg tcgcgcaccg aactcaccga acacatttac gcccaggatt
     4801 tcgagcgcga ctccaacacc atcgaggtct tcgtgggccg cctgcgccgc aagctgccgc
     4861 ccgaactgat cgaaacggtg cgcgggctgg gttaccggct ggtgccgccg ccatgaacct
     4921 ctggcgccgg gccacgtccc tgcgtttgcg gctgttggcc gcgacgctgg tggcactggc
     4981 gctggcgctg ctgctcgcgg gcgtgctgct cagcggcctg tttcgcgacc acgtgatgcg
     5041 gcagttcgag gctgccctga cgcaacagct cgaccaggtc accgcccggc tcgaatttga
     5101 tgccgccggc cagccgctga tcgaccccca gaccctgtcc gacccgcgct ggcaaaaacc
     5161 ctattcggga ctgtactggc agctggaccg caccaccgcc gatggtctgt cccgcgtggg
     5221 cgtgttgcgc tcgcgctcgc tgtgggacac cagcctgccg ctcctcgccg acgaactggc
     5281 cgacggcgcg gtgcatgtgc accagggcaa gggc
//
LOCUS       DAIDFG010000216         5269 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_216_length_5269_cov_17.062908, whole genome shotgun sequence.
ACCESSION   DAIDFG010000216 DAIDFG010000000
VERSION     DAIDFG010000216.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_216_length_5269_cov_17.062908"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1051 a         1639 c         1641 g          938 t
ORIGIN      
        1 tgccggtgaa accaccgaac atctgcggga agacaccgtg gtggtgccgg atccggccga
       61 tcgtgcgacc attgaagaag aacacgccct cgagctgcgt acgcgcgacc gtgagcgcaa
      121 gctgctcaag aaaatcgagc agtcgatcgc ccgcattgac tccggcgact acggttactg
      181 cgacgaaacc ggggaagcca tcggcgtggg gcgcctgatt gcccgtccga cggccaattt
      241 gtcgctggaa gcacagcagc gtcgcgagct caagcagaaa atgttcggcg actgagcttt
      301 cccgttgccc acttcctcct gaaccaccgt tgatggccaa agaagacacc acacctggct
      361 tgctttcaaa agtcgtgaag ttcgtccgca accctgcgac gaactggtcg gaacttgaag
      421 gcaaggagtc ggaccgggag gaagccctga gcaagcagat gctcaaggag atgattgagc
      481 gaaaacggcg caacgatttc gtgcgcaagc gcgaattcga catgctccgg aaaatgcgca
      541 agcgggaggc catgactggc caggaccccg gtgcccggcc ttcattcttc cagagcagca
      601 tgccgtccaa gcctgacgac cgcgccgtca ccctgaagaa aatcgacgaa atcgaggccc
      661 agatgtccat gcaatggtgg aaaaccaagc atggaagtgc ggccgctggc gccagcgagc
      721 cttcttcgct ttccccggcg acgacggccg gtcagcccaa cctcagcacc cacgccccgc
      781 tgctggacaa cgcgttgccg gccaactttg cgtccacggc gcctgatgcc ctggctgcgg
      841 cgctggccaa aaccagggct gccgctgtgt cgccgcagcc cgcaccggcg cccatggcgg
      901 acctgtccgc cggtgtgacc gctacgccgc ctgctgctgc acccaagcca gctgcatcca
      961 gaccggtcat ttctgcagcc tcccgcgatg gtgaaccgtc gggtttttcg gcgtccaaat
     1021 tttccgtggt ggatgcctcg gaggtggcgc acgaccccga actggaggag gcctccatcc
     1081 gttttgccaa tggcgacgat gcaggcgcag aagccgggtt gctcgagacg ctgggccccc
     1141 agggaacccg ggccaggcat gctgacacct ggctcgcgtt gtttgacctg taccgggcca
     1201 ccggacagca cgacaagttc gaaaacctgg ccgtcgagtt cgtcggccgt ttcgaccgtt
     1261 cggccccgca gtggttttcc atgcccgaga tggtgcagca gttgtccaat ccggccagct
     1321 cagccggtcc ggccgctggc aacggaccgg ctgcggactg ggtctgcccg tcaaaactgg
     1381 gcatccagac cgtggcggcg ctcagcgcgg ccctggcccg cgctcccatg ccctggcgac
     1441 ttgactggaa gaacctcaaa acgattgatg aaaacgccgt ggaacccctg tgcaaggtgt
     1501 tcagcggctg ggccgcacaa cccgtgcagt tgcgtttcat gggggagacg caattggagc
     1561 gggtgttgca ggacgcgacc ccctcagggg tgcgggacac gtcccaggga tggtggcaac
     1621 tgcgcatgga gaccctgcgg gtaacccacc agcctgacga gtttgagctg acagccctcg
     1681 atttttgcgt gacatacgag gtttcgccac ccgcctggga cagtgcgcgc tgcgactaca
     1741 agccgctgga gccgcgcggc gccgctatgg ccgggcaggc catcattggc gatgtctacc
     1801 gcgattcggt gccgtccagc ctcagcagca cgatggaggg tgacaccgag atgggcggga
     1861 actcccagat gagtcatctg tatgcggtcg aactgtctgg ccagatcgag ggagatgcca
     1921 ttgccgtcct cgacaagctt gaggccaagc tgatgggcgc agacatcatg gtgatttcgt
     1981 gcgcaaagct gattcgtgtt gatttttctg cagccgggac cctgctcaac tgggtttccg
     2041 cccgggaagc cgaaaatcgc gccgtgcagt tcaccgatgt caaccgtctg gtggccgcat
     2101 tcttcaacgt gatcggcatc accgaacacg cccgggtcct gacgcgcatc gactagggcc
     2161 tgtcccccat ccgcgagggg acccaaaaaa cgccatcgtt gccctgcgca acacgcagtc
     2221 tgtctgcgcc gggcaaccgg caggcagcgc tgcaggtgcc ataaccaccc aaggggcctt
     2281 cgaggtctct tttctccttc actctcccgc gtggcgcccg cgccaacgcc agcccggttt
     2341 gcttccggct taaagcaaca ctttcactgg atcttctaag tgcttaattt agaacgattt
     2401 ttaattgatg tctgcttgca aaatcgtttc taagtgcttg atttcattga aaaaatttgt
     2461 agcaaaccac ttgcatggca aaactttcct gctaaagtgg gaaaaagtgc aatttagtgg
     2521 tgaaaagtgt tctctaaagg tttttaacgg tgtttcaagg cgcgtcttct ctagctctcg
     2581 ataccaaggg tcggctctcc gtgccaaccc ggcatcgtga cgtgctcagc gccaccgcat
     2641 cgagccagct gaccatcacc aaacacccgc acggctgcct catgatattc ccgcgcaacg
     2701 agtgggagaa attccgcgaa cgcattgctt cgctgccgat gcaggcacag tggtggaaac
     2761 ggattttcct gggcaacgcc atggacgtcg acatggatgc cgccgggcgc gtgctggtct
     2821 ctcccgaatt gcgtagcgcg gcaggcatca gccgggacac ggtgctgctt ggcatgggca
     2881 gctacttcga gctgtgggat gcggcgacct atgcggccca ggaggccgag cagatgaagg
     2941 gcgaaatgcc cgatgtcttc agagactttt cattctgaaa gacgacggtg caagacacat
     3001 ggacacaccg caccgtcttg ttgaacgaag cagtcgcagc gcttcagatc aacccggacg
     3061 ggcattacat cgacgcgacc ttcgggcgcg gcgggcactc gcgcctcatt ctttcgcagc
     3121 tgtccgagct ggggcggctc accgccttcg acaaggaccc ggaggccatt gccgaggcga
     3181 agaccatcgc ggacccgcgt tttgccatcc gccaccaggc cttcagtcat ctgggcgaaa
     3241 tgccggcggg cagtgcggcg ggcatcctgc tggacctggg catcagctcg ccgcagatcg
     3301 acaatccggc gcgcgggttt tccttccgcg gcgacgggcc gctggacatg cgcatggaca
     3361 gcacgcgcgg ccagagcgtg gccgagtggc tggccgatgc agagacggac accatagcgg
     3421 aggtcattcg tgaatatggc gaagaacggt ttgctgggca gatcgcaaag gcgattgatc
     3481 gtcgccgaca ggaacggggc cttattgcaa ccaccgctga actggccgag gtcgtggctg
     3541 gcgcggtcaa aacccgcgag ccgggcaagg accctgcaac gcgcacattt caggcttttc
     3601 ggattttcat caatgccgag cttgaagagc tgcaacaggc gctagaggcc tcgctcaaag
     3661 tactgcaacc gggaggacgt ttggtggtga tcagtttcca ttcgctggaa gaccgcatcg
     3721 tcaagcaatt catcgccgaa cattcgcgcg aggtgttcga ccgccgcgcg ccctttgctg
     3781 cgcccaaggc catgcggctc aaggcgctgg aacgtgtcaa acctggcgcc gacgaagtgg
     3841 cggccaaccc gcgctcgcgc agcgccgtga tgcgcgtcgc cgaaaggacc gaggtgccgg
     3901 ttcaggtgag cactcagcgt taccctgggt tctcagcatg acacgcctga atctggtcct
     3961 gctggtggct gtactggtca gcgcgctgta cctggtgcgc acgcagtacg aatcgcgccg
     4021 gctgtttgtc gagctcgaaa aaacgacggc actcagccgc cagctcgagt ctgacaagga
     4081 gcgcctgcag gttgaaaagc gcgcacaggc cacgccgctg cgggtggaac agatttccag
     4141 ggagcgcctg cagatgcgct cggccacacc ggccatcacg cagtacgtga cctacaagaa
     4201 cgcagcagtg gacgggagcc cgccatgagc aggagcgtgc gttacacctc cagccccctg
     4261 ctggccagca agacgccggt ctggcgcagc aagctgatcg tggccggcat tgcgctggcc
     4321 tttgccggtc tggccggccg cgccgcctat gtgcaggtgg tcggcaacga tttttttcag
     4381 cgccagggcg aagtgcgttt tgcccgcacg ctggagctgc cggccaatcg cggacgcatt
     4441 ctggaccgca acggcctgat cctcgcgtcc agcgtgcccg cgcccagcat ctgggccatc
     4501 cctgaagacg tcgacggcag caaggcccag ctcgccgagc tggccaggct gctgcagatg
     4561 cccgtggccg agctcaacaa aaagctgtcg aacgaggaca agagttttgt ctggatcaag
     4621 cggcaggtgg atgcgtctgt cggcgaaaaa atacatgcac tcggcatcaa gggcatctac
     4681 cagcgcaagg aatacaggcg cgaatacccg gaaggtgaag ccgctgccca tgtggtgggt
     4741 ttcaccaatg tggaggaccg gggccaggag ggcatggagc tggcgttcaa caaggacctg
     4801 gccggccacg ccggctcgcg ccgggtgatc aaggaccgcc tgggtcgtgt cgtggaagac
     4861 gtgcgcgaac aggtgccgcc ggtggacggc aaggacctgc agctgtccat cgacagcaag
     4921 atccagtttt ttgcctacca gaagctgcgt gacgcggtgc tgacccacaa ggccaaggcc
     4981 ggcagcgtgg tggtgctcga cagcatcagc ggcgaggtgc tggccctggc caactacccc
     5041 agcttttctc ccgacaagcg ccagagcctg agcggagcgc aattgagaaa ccgcgcactg
     5101 accgacacgt tcgagcccgg ctcgaccatg aagccttttg tcgtggcgct ggcgctgcaa
     5161 aaaggcctcg tccgtccgga gacgcagatc cagaccgcgc cgggccgcct gacgattggc
     5221 ggttcgacca tctcggatgc gcacccgcaa gggctgctca cggtcaatc
//
LOCUS       DAIDFG010000217         5249 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_217_length_5249_cov_16.129765, whole genome shotgun sequence.
ACCESSION   DAIDFG010000217 DAIDFG010000000
VERSION     DAIDFG010000217.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_217_length_5249_cov_16.129765"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          780 a         1618 c         1846 g         1005 t
ORIGIN      
        1 cagtcttgca gcaggcgcac ggatggagat cttttttcat gaattcccga acatcggccg
       61 ctttggcctg gggtggcgtc ctgcttgcgg gcgtcttgtg gggtgtaggc gcgctggttg
      121 cccagtcggt gatggagggc ggcagcatgt cgccgttcag tctttctttg gcgcggtttg
      181 cattgggttt gccgctcttg tggtggtggc attggcggca ggtggccgcc tcaccggccg
      241 ccggtcaatg gcggcatttg ccgtggcggg agagggcgct gattgttgtc accggcctgg
      301 ccatggcgat gaacgtgacc tgctggttca tgggcgtcgc ctacatcggc gcggccttgc
      361 cgacggtgat ttcgatctgc tgcgcgccgg tgatcgtggc gctgctgtcg gtgttgcgcg
      421 ggtatgaacg cgccagcgga cggctgctgg cggcgctggt gctcgcgctg atcggcgtgg
      481 ggttgatcgt catgcccgcc gaagcgatca cactgccgaa agactatgcg gcgggcctgg
      541 cctggtcgtt tgcgtcagcc ggtttgtact cgctggtggt gctgggcaat gcgcgcatgc
      601 cggcgcatgt gccggccgtc agcgcctccg cctggggcat gacggtggcg gcgctgctga
      661 tgcttgtgct ggctggcgcg cagggcatga cgtggccgcg tgacggcacg gcctggttgc
      721 aggtggcata caccggtgtg gccaccacgt cagtcgccta cctggcgttt gcgtgggggg
      781 cgcgtcggct gtcacccact gccgccgtcg tgggcacctt gattgaaccg ctggtggcgg
      841 cgctggcggc cgcctggctg tttgccgagc cgctgacagc gcagcaaggt ttgggcggct
      901 tgttacttgg cgcggcgatg gtgctgctgg cgcggcgcag ggcagcaggc ttctagtccc
      961 agaacggctt ggcctgcagt gccaacaggt cgcaggcctg ctggcacgcc tgcacatgta
     1021 cctcgcgccg cgcgctgagc cagccggcac cgaacagggc gcgtgggtcg tggcctgaca
     1081 ggccttgcca gacctgcagc gccgccactt cgtcgtggta aatgcccagc gcgtcctgca
     1141 gcggcttgag ggccgccagg taggcggcgg ccttgcgtct ggcaaacagg ggttgtgcaa
     1201 attcggcgag gtaacgcagg cgcttgaggc gcttgcgcag cgcgtgttgc tcggcctcag
     1261 gcagggccgt gaattggcgg ccctgcacca gcacctgctt gtgcagtttg gccaggcgct
     1321 tgcgcaggaa tttttttggc gatgtgtcgt cgggcgcaga cgcgccctcg ccgggggcgg
     1381 cgtgaacaaa accgatcagg cccagcagtg cctgctggaa agccggcgcg cgtaccgact
     1441 cggcggtgct gggcagggtg cccagtgttt cctgccagtc gagcggcggc ccgcctgcgg
     1501 cttcgatctg accctgcagc ttgccgagca ggtagtcgcg gtcgcggtgc tggcccagtt
     1561 cgcggaaaac gctggtcagc gccgccttcc aggcctcgcc ctgatcgccc cagccgcctg
     1621 cgagggcctc gagttcctgc agtgcggttc gcagccggcg cagaccgacg cgcagctggt
     1681 gaatgttctc ttccgcgatg cagccggcgg ccacgtcgct ggcattggcc agcacctggt
     1741 tcaggcaggc ggccaccacc gcggcggcca cctcgcggcc cgaggcgtgg cggggaaagc
     1801 gcggcgcttc ggcggccacg gccgcgccga agtcctggcc cagcgccagg cgctggcctt
     1861 tcagggactt gctcacgctg ctgagccaca ggccatgcct gaggcaccag gtggtggcca
     1921 gggccacggc gccggcggct tggccctgct tgagttcgag ctccagctcg cgcacgggca
     1981 gcgtcctgtc gccggcggaa atgtggccct ggtccagcgc cacttcgacc agcgcgccgc
     2041 gcgcccgcac cagccgcgtg aggcgaacca tgtcggtggc gtagacaagg gtgagatcgg
     2101 gcgccgccgc gtcgggctgg cgcttcaggg cttggcgcag gcgctcgccg accggtgtgc
     2161 cgtcgtggcg cgtcaggtcg ggcaggggtg cggtctggct acccagcacg acgttgtgct
     2221 ccagccgctc cagcggccgg ctgccggggg ccttggccgt ctgtacccag tgccggcctt
     2281 ccttgcgcag gcgcagcacc acgccttgcg ccgccagtgc gcggtcggcc gtgtcgaagt
     2341 agcgtgcccg caggcgctgg gtgcgtgtca tgccgcggcg caacgccgct tccaccgaag
     2401 cgagccgggc gggcgggatt tcaagcttga gttcgaactc ggtcatggtg tggcctgcga
     2461 cagggtggcg cccccatcct acgcgcacgg gcggcgttgt gccggtttat gaggtcaaat
     2521 cggcctccag cccttattgg gcgggcgcca gcagctatga atctggtagc gcgtgtgcgc
     2581 tgcttcgcca ggcggcctgc gctctccttg acctgcagtt gccgcgtgcg tacattgcct
     2641 tgcaatcaat cagggaagga ggggccatgt cagaaggttt tgtttccaag ctgctgaatc
     2701 cgaaggatcg cctgggcgtg atgcgcctgc atgcgatctt gacgggtgac ctgggccgcc
     2761 agggtgaaaa gccggggcgc acggactaca gcaaggagct gctttcgccc aagaccaagt
     2821 ctggtttcga ctacctgcat gtggccaagg ccgacagggt gagctacttc gatgtacggc
     2881 ccgaggcgct tccggcaccg cacctcaagc tgcacatcga accgcagtct gtgtccgccc
     2941 agttcctgtc gcacaaaaag gccaaggtgt tgcggccgat gttttacttg ccctatgagc
     3001 agaacggcac cgcgcggatg acgctgtccg agcccgacgg ctataacggt gacgaagtgc
     3061 gtttttttgc caccgccacc atcgacggct gctcggttta catcgaaggc ccgtccaatg
     3121 cgcccaaggt gacacacatc aacgcagcgg ccatttcacc caccacgcgc gtggacccct
     3181 gggaccagaa gcagctgaag atccaggcca agatagcgga catggatgcg cgcttcgtcc
     3241 aggtgcaacg ccgcacgggg catgtgcttg agcggccgca ctacatggtg gaggaaaagc
     3301 gcgaactcaa ggcgctcaag aaacgttttg cccagaccaa aggcatcccg taccaacaga
     3361 taggcatgta tgacccgttt ggcgcggtgc ttggctttca ggacggcggc cactggactt
     3421 tttatgtgca aaaaagcgcc aagttcgact ggcgccgccg ccctgaggat cccccgcggc
     3481 tgatgcagag cagcctgtgg gtgcacgagg tgctggagat ctggccgaac gggcccaatc
     3541 actaccggca agtctgaagc caccaccggc cgatgtatgg cgcgccatgg agcgcaagct
     3601 ccagccctcg ccagccggtc gctaccagct attcattcga tagcgtgtgt agcgggatgt
     3661 ctttctccgc agccagtttg tccagttgtg cacgggtttg cgtgcgcatg aaagcggcga
     3721 ttgcctcgtg cgtggcgggt gcaaacatgg cgcggatgtc cgcggcttcc acgcccgcgg
     3781 ctgcgcacag ggcggcggcg aagtgcggct ccagcgcggc caccgccaca cggccgtccc
     3841 ggcaggggta gatgcggtag ccggcatgcg cgccaccgac cgagccgctg ggctgcgtca
     3901 ggccccattg gcgcggcagc gccaggtagg ccgcggcatc ggacagggcc acttcgaaaa
     3961 aggccccgcg tggatgcggg tcaccggtgc ctgcataggg ctcctgcctc cgcaaggcgg
     4021 cctgcagcac ggcttcgctg gccatcagcg agccgctcat gtcggcgtac agcgtggcgg
     4081 gcagctccag cccgggtacc aggccgttgt cggccaggta ggtcaggtcg tggccgggct
     4141 cttcagcgcg tgcgccgggg gcaccgacga tggcgacatg cgacagcgcg gggtacagcg
     4201 tgtggatttt tttccagccc aggcccagct tgtcaatcgc tgaaggacgg aaggaggtga
     4261 gcagcacatc ggttttttca agttcgcggt gcagtgcctt ctggccggct tcggttttca
     4321 ggtcggccgc cagcaccttc acgccttcat gcagctgcgc gtaggccggc ttgttgtaga
     4381 gacccatggg atcgcccgag ggcggctcga gcttgatgca ggtcgcgccc atctgccggc
     4441 aacgcatcag cgcggccggg ccgggcaggt tgagggccag gctcaggatg cggatgccct
     4501 gcagggcctg gaggggcggg ttttttttgg cgggggcaga ggaggtcatc gtggtggcgg
     4561 atggtctgga gggcaggaag gtagaaggcg gccagaggca attgacctta ccagactttg
     4621 cgcctgcggc ggaggctgcc ggtacccccc cccggatgcc agtcagtcct gtttcttttt
     4681 tgtaaaaatt aatgttttac aataaataat tattgtttta cagatatttg aagccataat
     4741 gggcgcctca agacacagga ggctcccatg aaaaacgcat tcgccgcaag caccctgaca
     4801 cgccctgacg tgcaacgcct gaacagtgcc tttgcctggg tttgcgccct gttggctgtg
     4861 gccttgccgc tggcggtgct ctatcagctg gtgaccacgc cgaccgaggg tttgttgctc
     4921 cgatcgggcg tcagcctgtc gacgctgcag gtggccatgc tggacgtggc gttgtggcaa
     4981 cgcgtgctgg ccgtggtact gggcatggtg ccggtgtgtt gcgcatccta cggtctggtc
     5041 tgcgccatgc gctgtttttc gggcttctcc cagagcgagt atttcagcct gcgcaacgtg
     5101 cgttatctgc gcggtttcgc cgcgggcatt tttgcctcgg tggtggcggg cttttttgtt
     5161 tccatggcca tcagcgtggt gttgaccctg ggcgcaccgg ccggtcagcg cgcactgtcc
     5221 atgggcctgg gcagcaacga gctgatgac
//
LOCUS       DAIDFG010000218         5230 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_218_length_5230_cov_20.508406, whole genome shotgun sequence.
ACCESSION   DAIDFG010000218 DAIDFG010000000
VERSION     DAIDFG010000218.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_218_length_5230_cov_20.508406"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          943 a         1690 c         1695 g          902 t
ORIGIN      
        1 gtttcaacgt catcctggtc acgcacgacc tgcgcgagtc ggtcttcctg gccgacaccg
       61 tgtatgtgat gagcaaaagc ccggggcgtt ttgtcgtcaa acgcgagatc gacctgccgc
      121 gcccgcgcga cctcgaggtg acctacaccc aggccttcgg cgacatcgtg cacgagttgc
      181 gcagccacat cggcgccatc cgcacgcccg tcatcaaccc ggttcccgcc gctgaaggca
      241 aatagcatga gcaaaaaaca gattgagcgc tggtccccgt ttgtgctgct ggcagccatc
      301 ctggcgctct ggcaaggggt ggtcatggtg ttcggcatcg ccgaatacat ttttcccgcg
      361 ccgctggcga ttgcccaggc catggccgag ttcgccggcc cgatcgcgca ggccgcctgg
      421 aaaaccttct gggtcaccat ggtcggcttc ggcttgtcca tcgtggtcgg cgtgatgctg
      481 ggttttctgg tcggctcctc gcgcctggcc tatgccgcgg tctatccgct gctggtcgct
      541 ttcaacgccg tgcccaaggc ggcgattgtg cccatcctga tcgtctggtt cggcatcggc
      601 gtcggccccg gcatcctgac cgccttcctg atttccttct tcccgatcac cgtcaacatg
      661 gccaccggcc tggcgacgct ggagcccgag ctggaggacg tgctgcgcgt gctgggcgcc
      721 aaacgctggg acgtgctggt caaggtcggc ctgccgcgct ccatgcccta cttctacggt
      781 tccctcaaga tcgcgatcac gctggccttt gtcggcaccg tgctggccga aatgacggcc
      841 ggcgactccg gcatcggcta cctgatgctc agcgccggct cgcagcaacg catggcgctg
      901 gcttttgccg ggctggtggt gatcggcgcg atggcaatga tcatgtacga gctgttcagc
      961 tacatcgaga aacacacgac cggctgggcg catcggggca cgcagggcgg ttgaggccgc
     1021 cagctccggt ttacgcatga aatcgggctc cagcccaagt aatacgtggg caaccagcta
     1081 ttaaaatcat agcgaaatgc atccatacta cgggtgcctc cgtcatcctt gacttcatcc
     1141 cacccctttg cggcctgaga ttggcggatg gagccgcctc agtgaagggc attgtctgac
     1201 agaggcgcgg cctgcccgac gactcgcccg gtgcaggtgc cggagttaca ccagagtggt
     1261 agcgcttatg gcgctcatgc cctcgtcagt caacttccaa ggagcgactc atgtccccat
     1321 ccaccacctc tgccacccgg ccgcttaacg ttgatccggc agacaaagac cttgccgagc
     1381 aggcgcggcc cggccacggc attccgtcgc aggatcctga acctgcggcg cagcttcccc
     1441 tcacggcggc agagtccgac cgcgaatcca actctgtatt gatgggcggc ggcatgatcg
     1501 ccggtgcggc taccggcgca gccgttggcg ccgtattggc cggcccggtc ggtgtcctgg
     1561 tcggcggcac cgtgggtagc gtggctggcg cactgggcgg agccgctgcg ggcccgttgg
     1621 tgacaccgga agatactgcc agcaccctcc ctgagccgac cgacagggaa gccaacagcg
     1681 ccaagcgata gaggtcagag aaagcgctcg agcaagcgcc gggaagccct gtccagcgcc
     1741 cccatgtcct tcacactgaa atgcacgcca tggccggcgg caatcagcag cgactggcgg
     1801 ctcaggcggc ggatgtcttc ctcgcccttg agcacgaacg aggtgtcgcc gcggtcggtt
     1861 tccaccgtcc aggtgctggg tgtgccgaag ctcgacacgc tgatgaggcg cgtgatctcc
     1921 ggcacaaaat cacgcagcgc gagttcttcc tgcagcaagc tgcgctgcag ctgcggcaac
     1981 tcgctcagcc gttccagcca gaccagttca tggccttcgc tgctgatcag ggagatgcct
     2041 tcgtcgggcg cggcaatcgg gaaagcgcgc accggcacca cgcccaggtg ctgctcgccg
     2101 tcaggggcaa caaccagcag cagctggccc gaggcgtgcc gctcgagctg gaaagcaggg
     2161 ctggcagaaa tggagggggt ggtcatgcgg agtccttcag acattgccgt tgcgctgcgc
     2221 ggtgtgggcc acctgcgagg tggccagcgc gatgcggcct tcctcggcat cggcattgcc
     2281 ctcactgtcg acctggcgcg cctgggcttc gtagagccgc cagtaggcgc cctgcttcgc
     2341 catgagttcg tcgtgcggcc cgacctcaac cacttggccg cggtccatca ccaccaggcg
     2401 gtcggccttg cgcagtgtgg acaggcggtg cgcgatggca atggtggtac ggccctgcac
     2461 caggttgtcg agggctttct ggatttcctg ctcggtctcg gtgtccaccg acgaggtcgc
     2521 ctcgtcgaga atcaggatgc gcgggtcgat cagtaacgcg cgcgcgatcg agatgcgctg
     2581 gcgttcgccg cccgacaggc cctgcccgcg ctcgccgacc agcgagtcgt aaccctgcgg
     2641 caggcgcagg atgaactcgt gcgcatgggc ggcacgggcc gcggccacga tctcggcacg
     2701 gctggcgccg ggtttgccgt aggcgatgtt ttcggcgatg gtgccaaaga acagaaaggg
     2761 ttcctgcagc accaggccga tattgcgccg gtagtcggcc acggtgaagc gccggatgtc
     2821 ggtgccgtcc acactgatgc tgccgtcggt cacgtcgtag aagcggcaga tcaggttgac
     2881 cagcgtgctt ttgcccgagc cgctgtggcc gaccaggcct atcatctcgc cgggctgaat
     2941 ggtcaggtcc aggccgcgga tcactgcgcg gttgccgtaa cggaagccca ggcccaccat
     3001 ctcgatgcgg ccctgcatgg gcgccatggg caccgggttg gccggttccg gcacattcga
     3061 ggcgtggtcg aggatgtcga aaatacgctt ggcaccggcc gccgcctttt gcgtgaccga
     3121 gacaatgcgg ctcatggagt ccagccgcgt gtaaaaacgg ccaatgtagg cgataaacgc
     3181 cgtcagcaca ccgaccgtga tctgctgctt cgacaccagc cagatgccga aggcccagac
     3241 caccagcagg ccgatttcgg tcagcagcga gaccgtcggc gtgaacagcg accagacctt
     3301 gttgagcttg tcgttgacca gcaggttatg ccggttggca tcgcgaaaac gctgggcttc
     3361 gcgcttttcc tgggcaaagg ccttgaccac gcggatgccg ggaatggtgt cggccagcac
     3421 attggtcact tcggaccaga cccggtcgat tttttcaaaa ccggtacgca ggcgctcgcg
     3481 caccgcatgg atcatccagg cgatgaaggg cagcggcagc agggtcacca gcgccagcca
     3541 ggggttgatc gaaaacagga tggcggccgt catcaggatc atcagcacat cggtcgcaaa
     3601 gtcgagtgca tgcaacgaca ggaacacatt gatgcggtcg gtttccgagc cgatgcggct
     3661 catcaggtcg cccgtgcgtt tgccgccgaa atagtccagc gacagctcca gcaggtggtc
     3721 aaacgtggcc gtgcgcaggt cggcgcctat gcgttcggac accagcgcca gcaaataggt
     3781 gcgtgcccag ttcagcgccc agccgagcag cgccgccgcc agcaggccgc ccaggatggc
     3841 cagcaccttc caggtctcga tctgctggcc gttctggaac gggatcagga tgtcgtccat
     3901 cagcggaatc gtcaggtaag gcggcaccag ggtcgcggcg gtggaggcca gggtcaacaa
     3961 aaacccggaa agcagctgcc actggtaagg ccgggcaaag cgccacaggc gcagcaagac
     4021 ccaggtcgac ggcggcgtgt gcagttcgcg ggcgcaggtc gggcactcgt cgctgtccgt
     4081 cgccagcacc gtatcgcaaa aggggcacag ggccatgtcg tcgctggcgg cagccgcacc
     4141 gggcgccaat tgctgctcga acaggcgcag cagccgcagc gcctgcacat tggccgccag
     4201 cgtaaaccgc cagttggcca ggcgctgccc cccctggtga agctccagcg tgccgacacc
     4261 ggcatggtcg aaatggcgca gcgacagggg cggcaggtgg ccggaggcct ccgccgggcg
     4321 caacggccag ctggcccaca ggccggtttc cgggtcgcgg gccatcaggc ggcggtcggt
     4381 cagagccagc aaaccccggg aaaaacgaag ttgctgattc aggtcaacca gcaccgtaca
     4441 taacacgttc tcatggtttg cgagttgcgc gcgcaggtcc gtctgcgctg aaccagcctc
     4501 gctggaggcg tcgaccggag aggaatgttg cattgtgatg tggaagccgt aaaaaccggt
     4561 gccccgaaac ggtggggcca aatcagggat tttcgcccaa tccgtgccgg ctgatgcgag
     4621 attcggacac cggaccggaa aacctgcccg aacaaacaga aacgcccgtt gtgggcgctt
     4681 atccagaaaa gaacgttgcc ccggcccgac cggttgaccg aaatcagaaa aacatgagca
     4741 aacaggacga catccagctt ctgcatatca actacctgcg cggccccaac atctggactt
     4801 accgccctgt gctggaagtc tggatggacc tcggcaccct ggaggacttt ccctccaacc
     4861 tgctaccggg tttcagcgac cgcctggtcg cactgttgcc tgccttgctg gagcaccact
     4921 gcggcgtcgg ggagcgcggc ggcttcatcc agcgcctcta cgagggcacc tgggccggcc
     4981 atgtgctgga gcatgtcgtc atcgaactgc tgaacctggc gggcatgccc accggtttcg
     5041 gccagacccg cagtacctcg aagcgcggca tttaccgcat ggtggtccgc gcccgcgacg
     5101 agcaggtggc acgcagcgcg ctggccgaag gccaccgcct gctgatggcc gccgtcaaca
     5161 acgaggcctt tagcgacgcg gacctgcagg cggcggttgg ccgcgtgcgc gacaaggtcg
     5221 acgagtgtta
//
LOCUS       DAIDFG010000219         5228 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_219_length_5228_cov_18.497970, whole genome shotgun sequence.
ACCESSION   DAIDFG010000219 DAIDFG010000000
VERSION     DAIDFG010000219.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_219_length_5228_cov_18.497970"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          958 a         1775 c         1596 g          899 t
ORIGIN      
        1 gccggctccg tggccccggc tggcgaagag aagaccccca cgcttatcgc ctgctaactg
       61 acgccatggc caccaaaacc acacccccac gttcggcgac tgcccggctc gcctcagcaa
      121 agcggccggg cgtgcgggag caggccgcgc aaaccacgcg cgacaacatc ctgcgtgccg
      181 ccaccaaggt ctttgcgcgg tatggctacg agggcggcag cgtggaaaaa atctcgcagt
      241 cggccaagtc cttcgaccgg atgatttact actacttcgg gagcaaggaa ggcctgttca
      301 tcgaagtgct ggaagacatg taccgccgga tgaacgatgc cgagctggag cttgaccttg
      361 acctggagca gccggtggag tcgctcaagg acgtcatccg ttttgtcgtg acctattaca
      421 gcaagaaccc cgagttcatc acgctgctca acacggaaaa cctgcaccgc ggcaaacaca
      481 tcgccaagtc catgcgcgcc cgggagtact cgtcacccgc gattgcgatc atccagaagt
      541 tactggaaag cggcgtgaag aaaaaggtgt ttcgcgctga catctccgca cgaaatgtgt
      601 acctgctgat cgcggcgacc ggctacttct acatttccaa ccgccacacg ctgtcggcct
      661 tcctcgggga agacctgcag actcccgagg ccctggccgc ctggcaggct ttcatcacca
      721 gcaccgtgct gcgcaccgtg atgctcaacc cctgacgcgc ccgcgccggc ggcggacatt
      781 cccacccacg acctttgaga gggcaaacaa catggcagaa gctgtatcga ccggaaaatc
      841 cggaaaagtg gtgatcaaaa acatcggcct gctgctgtcc ggggacatcg acaaacccat
      901 cctggatgcc gacacgattg tcgtgaacga tgggctgatc gtcgccgtcg gcaaggccaa
      961 ggactgcaac accgagggcg cgcagaccat catcgatgcg caccagacct gcgtctgccc
     1021 tgggttgatc gacagccatg tgcatccggt attcggagac tggacgccgc ggcaaaacca
     1081 gatcggctgg attgactcca cgatgcacgg cggcgtcacc accatgatct cagccggcga
     1141 agtgcacctg cccggccgcc ccaaggacat cgtcgggctc aaggccctgg ccatcaccgc
     1201 acagcgggct ttcgacaact tccgccccgg cggcgtcaag gtgctggccg gcgcccccat
     1261 catcgaaaaa ggcatggtcg agcaggactt caaggagctg gccgaggccg gcgtcacgct
     1321 gctcggtgaa gtcggcctgg gctcggtcaa ggccggctac gaagccaagg aaatggtggc
     1381 ctgggcccgc aagtacggca tccagagcac catccacacc ggtggcccat cgattcccgg
     1441 ctccggcctg atcgacaagg acgtggtgct cgaagccgac gccgacgtga tcggccacat
     1501 caacggcggc cacaccgccc tgtccgagca gcatgtctgc gaactctgtg aaaagtcctc
     1561 gcgggccatc gagatcgtgc acaacggcaa cgagaaggtc gccatcgcgg cggcccaggc
     1621 cgcgctgcag ctcaaatgcc cgcaccgggt aatcctcggc accgacgggc ctgccggctc
     1681 cggcgtgcag ccgctgggca tgttgcggct catcgccctg ctgtccagcc tgggcaacat
     1741 tccggccgaa ctggccttct gttttgccac cggcaacacg gcgcgtatcc gccagctcaa
     1801 ctgcggcctg atcgaggtcg gccgcgacgc cgacttcgtg ttcatggaca aggcccaaca
     1861 ttcggccggc ctgaacctgc tcgacagcgt gcagcgcggc gacattcccg gtgtgggcat
     1921 ggtgatgatc gacggcatcg tgcgcagcgg ccgcagccgt aacacgccgc cggccacgga
     1981 aatccccagc gtcgtcaccc cggcctgagc gccggcaccc cgcatgtcgc acctgccgtc
     2041 tccgaccaag gaggagcttg cccggctcga gcccctggcg cagctcggct tggccgatat
     2101 ttctgtggtc tccacgcgcc agcaggccga tgaagccttc cagtcactgg cgagcagcgc
     2161 cgtgctgggc ttcgacaccg aatccaaacc caccttcttc cgggaccagg tgtccgaggg
     2221 cccgcatgtc gtgcagtttt ccaccctgca caaggcctgg gtgttccagc tgtatgacgc
     2281 cggatgccgc gacaccgtgt cggcccttct cgagctggaa acggcagcca aggtgggctt
     2341 cgggttggcg ggcgatcatc agcagatccg gcgcacgctg ggtgtttctc cgcgcaatgt
     2401 gctggatctc aacgcggtgt tcaggagtcg cggctacgcg aaggagctcg gtgtccgcgg
     2461 cgcggtcgcc gtggtgttca accggcgttt catcaagtcg agaaaagcca ccacctcgaa
     2521 ctgggccaac cggcagctca ccgagagcca gatcgtgtat tcagccaacg acgcctgggc
     2581 ggcgatccag gtctttcacg cactgggcct gccaccgccg caggcctgag agactcaagc
     2641 gtaaatgaat tgctgtctga aggcggcgca gaactgtggg ctgccgccca tcgacagggt
     2701 cagcgtgtac cagcggtcgc ccgcctcctc ttcctgctcc tgcaggttga agtcccactc
     2761 cccgccatcg tccagcggcg cctgcaggcc cggaaaggcc gagtgcgccc actccagcac
     2821 ctgtacgatt tccgtgcgga cggcggccac ctggtcgggc cggacagacg ccatcgcgtc
     2881 gaaagtcccc acgccctgcg cgtcttcgct gtagtcgaac tcgagaaaat gcaaagggag
     2941 cttcaggacg acgcctcctg cccgtccgta ctttccgcgg gggccgcggc gggtctggcc
     3001 gtgcggtgcc cctctttggc gagcagttcg atgtaaaacg cagcgccgcc ttccgcggca
     3061 accgcgtcga tcaagcccat gatggtcgca aaatgcacca cggcggcgtt gtccgggctg
     3121 gtgccgaaac ggcagtcgaa atcccagtag tccacgctct cgggcagagg cacccggcgt
     3181 tgccttttga cgtatttgcg gatttcgtgc ttgacggcgt cgagcacgcg atcccggttt
     3241 ttgccttcga tgttgagctg gaatgtcttt ttcacgcttg cctttcgagg gggtctggta
     3301 tcagcccgga cggacgtgat tcgcaagcct tcgattatgc ggccccggcg gcggcccctt
     3361 gtggcgcaac agcttaaact gggcctttgc cgccgccccc tcacaaagcc ctacttcatg
     3421 ccattttctt ccctgggcct gtcccccgcc ctgctgcacg ccgtcagcac caagggttat
     3481 ctggcgccca caccgatcca gaccgctgcg attcccgcca tcctgcaggg ccgtgatgtt
     3541 ctgggttcgg cgcaaaccgg ctcgggcaag acggcagcct ttgtgctgcc gttgctgcaa
     3601 caactgctgg cgggcccgca cgagaagccg cgcagagtac gcgcgctggt cctggttccc
     3661 acacgtgaac tcgcggcgca agtcggcgag accgtccgca gcctgacgca gcaactgccg
     3721 caggccctga aggtggcgac tgtgtttggc ggcgtgtcca tcaacccgca gatgatgaac
     3781 ctgcgcggcg gggccgacat cgtggtcgcc acgccgggcc gcctgctgga cctggtggac
     3841 cacaacgcac tggccctgtc cggcatcacg gcactggtgc ttgacgaggc cgaccggctc
     3901 ttcgagctcg gttttgccga cgaactcgga cgcattctcg ccctgcttgc gccacagcgc
     3961 cagaacctgt ttttttcggc caccctgccg cccgccgtgc aggccctggc gcagacgctg
     4021 ctgcgcgagc cggtccgcat cgacgtgctg gccggaccgg agcaggcgcc cgacatcgtg
     4081 cagcgcgcca ttgccgtgga caccggacgg cgcacccagc tcctgcgcca cctgatccag
     4141 acaaacggct ggcgccgggt gctggtcttc gtggccacca aacatgcggc cgaaatcgtg
     4201 gccgacaagc tgcgcaaggt gcggatcaac gccgagcctt tccatggcga actgagccag
     4261 ggaaaacgca cgcaggtgct ggccgacttc aaggcctccc ggctcaaggt ggtgatcgcc
     4321 accgatgtgg cggcccgcgg cctggacatt gcgcagttgc cggtggtggt gaactacgac
     4381 ctgccgcgtt cggcggtgga gtacacccat cgcatcggcc gcaccggccg cgccggagaa
     4441 agcggcgtcg ccctgagttt tgtcagcgcg gacacgctgg cgcattggcg cctgattgaa
     4501 aaacgccagc agctggcgct gccgctcgag cagatcgaag gttttgaacc gctgccgacg
     4561 gacccgtcaa ctgacgaagc cgccagcccg ccctcgacgg gcggcatcaa gggcaaacgc
     4621 cccagcaaga aagacaaact ccgggccgcc gcagcgcgcg gagactaggc gttttcagtg
     4681 accggcgccg ttgacgctgg tgatggcata tcctttttcg gcaatttgct gctcgggacg
     4741 gggaatcgcg tactggctga tcttctcggc gctggcaccc atttccacgg cgcgggcttc
     4801 ggactcccaa tcgctgttcg tggcctcctc gggaatcagc cgcccctcgg gcacagcgag
     4861 gtaggaaaac tggcgattgg cttcggcccg gcggtaaatg tcgacacgca tgaggtctcc
     4921 ttggcaggtg gctgagtcct ccaccttacc cccggcaagt gcctgcctgc aggaaagtcc
     4981 ctgcttggta cccggactcc cccggccggg ccgattcgga cacgacgcca ggccccgaag
     5041 cgagaacctc aggaactcct tttttgatag ctgactgcgc ttgtacatca agggctggag
     5101 ccacttttta tcgtcaaatt gtgtgccaaa aataccaacc ccatgcagcg tagacacgca
     5161 ggtgacgcct tcgccgccgc gctggcgata agctgccacg caagcctttc cctcccccca
     5221 cagacagg
//
LOCUS       DAIDFG010000220         5201 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_220_length_5201_cov_20.977264, whole genome shotgun sequence.
ACCESSION   DAIDFG010000220 DAIDFG010000000
VERSION     DAIDFG010000220.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_220_length_5201_cov_20.977264"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          861 a         1687 c         1790 g          863 t
ORIGIN      
        1 aacaagcgca tcggcctggt gtggagcggc agcaccggcc accagaacga ccacaaccgc
       61 tccctggcgc tggcagacat gcggggcctg ctcagcgagc gggccgagtg gtactgcctg
      121 caaaaagagc tgcgacctgc ggatcaggcc acgctggcaa acacgcccgg actgaccttc
      181 ctgggcgatt cattccagga cttcagtgac acggccgcgg tggtggcgtt gatggacctg
      241 gtgatcacgg tggacaccag cgtggcgcat ctggcgggcg ccatgggcaa agaggtgtgg
      301 ctgctgttgg cctacagccc ggactggcgc tggctgctgg ggcgaagcga cagcccgtgg
      361 tacggctcag cgcggctgtt caggcagagc gcccctggcg actgggccgg tgtgctgacc
      421 caggtacagg cggcgttgac gctgcgcctg cgatagggag gagcccctat tgccgggcgc
      481 cgcgtgcgag cgctgcagcg cgtgacgcgg ccagcgcctg cacgtcgggc acgccctggg
      541 tcggccagcc ggacaggtgc tgctgcgcaa tgtcgctcag ggccttcagc cattcggggc
      601 tgtcgttgag gcaggcgatg tagttgaagg tcttcccgcc cgcatggaca aaagcctcct
      661 tggcctcctg cgcaatttcc tcgagggtct cgaggcagtc gccggtgaag ccggggcaca
      721 tcacatcgac gctcttgacg cccgccctgg ccagggccac cagcgtgggc tcggtgtagg
      781 gctccagcca cttggccttg ccgaagcgcg actggaaggt gacgcggtac agctccttgg
      841 ccagtccgag ttcttcggcc agcaggcgag cggttttatg gcactcgcag tggtaggggt
      901 cacccagatg cagcgtgcgc tcgggcacgc cgtgaaaact catgaccagt ttttctgcgc
      961 ggccatgggt tgcccagtgt gcgcgcacgc gcgctgccag cgccgcgatg tagcccggat
     1021 ggtcgtgata gtggttgacg aaccgaaact cgggaatgcg ccgtgttttg gcggcccagc
     1081 tgtaaaccgc gtcgaacacg ctggcggtgc tggtgccgct gtactgcggg taggcgggca
     1141 acaccaggat gcgtgtcacg ccttcggcct tgagcgagtt gagctgcgcg gctacggagg
     1201 gctggccgta acgcatggcg tagcgcactt caacatggtg gccgcgctcg cccaggtagc
     1261 cgcgcagcag cgtcgcctgt ttggccgtcc acactttcag cggtgaaccg tccgcggtcc
     1321 agatgctggc gtatttggcc gctgactttt tgggccgcac gcgcaggatg atgccgtgca
     1381 ggatgaacca ccacaaggcc ttggggattt cgatcacacg gtggtcactg agaaactgcg
     1441 cgaggtaggg gcgcagcgcc gccgcggtgg gtgcgtccgg ggttccgagg ttgcagtaca
     1501 gcacggcggt gccgctggcc gcgctgggcg gctgcccgtg cacaaaggtg ggttcggggg
     1561 aaaaagacat gggttattct cccttaccta tgcttgacct gacaattatt aaagccataa
     1621 cccttgacct tgatgacacc ctctggccgg tctggccggt gatagagaaa gccgaagccg
     1681 cactcgacga atggctgggt cgccacgcgc ccatgaccgc ggccctgttt gccaacccca
     1741 cggcacgcca cgatatccgc gagcagattg tccgtacccg gcccgagctc aggcacaacc
     1801 tcagtgccat ccggcgcgag gcgatccgcc tggccctgta ccgttcccgc gagaacccct
     1861 tgctggcgga agaggccttt gaagtttttt ttgccgcgcg ccatgacgtg acgctgttcg
     1921 acgacgcgat gctggcgctc gagtttcttt ccgcgcgtta cccggtggtt gcgctgtcca
     1981 acggcaatgc cgatatcggc cgcatcgggc tcgcgccata ctttcgcgcg gccatcagcg
     2041 cgcaggagtt cggcgtgggc aagccggacc cgcggatttt ccatgcggcg gccggcgccg
     2101 tggatgtgca gccccacaac gtgttgcatg taggcgacga cgcagcgctc gatgtgctgg
     2161 gggcgctgaa cgcgggcatg cagacggcct gggtgaaccg ggccgaccat ctgtggacgc
     2221 acgatgccgt gccgcacgaa accgtgacca gcctgaccga gctgtgcgac ctgtttcgcc
     2281 tggcggaccc gcagccatga cactgaaggt ctacggcatc gcggcctcgc gcgcatcacg
     2341 gccgctgtgg gtggcgcatg aactgggcct ggcctacgaa cacattccac agccttacca
     2401 gggcggtgcg acgcgcacgc ccgagtttct ggcgctcaat cccaacgggc acatccccgt
     2461 ggtggacgac aacggcgtgc tggtctggga gtccatggcc tgtgcgctct acctggccgg
     2521 gcgtttccag ggcgagggcg ctgaaccgct cgccgcagtg aaccacgccg aacaggccga
     2581 gatcctgcgc tggagcttct gggccgtgac cgaatgcgaa aaagatgccc tgacgttttt
     2641 gatgcacacc gtggccatgc ccgaggaacg gcgcaagccg caattggcgc aggaggccga
     2701 gcgtcgcctg tcgccggccc tgcgtgtgct cgatcagcat ctgcaaaccc gccggtttct
     2761 ggccggggag cgtttcaccg tggccgacat ctgcgtggcg tcggtgctgg cctgggtgca
     2821 gcgttcggcg gagctgaacg cgcagtgccc gcagctcagc gcctggctgg cgcgttgcct
     2881 ggaccggccc gcgcagcaac aggtgcgcgc gctggcacgg cgtgatagtg gccggaatct
     2941 ataggcaaat cgggctccag cccttgctgt tattgcgcaa acagctatct atttgatagc
     3001 gtttctggcg gcctgcaaac ccgaacgcac cgcaccttcc agcgtggccg ggtaggggcc
     3061 gtcgaggtag tcgccgcagg caagcagccc tggcagcacc tgcgcggcgg ggcgctgcag
     3121 gccgggcagg caggcgaagg tcgcgcgttt ttccacaatc gtctgcacgg gctgcagggc
     3181 cgccagcccg agttgctgct cgccctgggc cagcacgcgc ccggtcagtg cggccttgtc
     3241 ggcgctgctg gcgctgacca caaaggccag caggccggcg ggcccgccga gctggccgcg
     3301 atcaaacaca aactgcgccg gcgcctcgcc gtcgggtgcg ccggcgcgca gcgtgagcat
     3361 gggctgcgcc aggcgcgcgc cgggtgaaaa cgcatacacc gtggtgatcg cttcaaactg
     3421 cagcgcgcgg gtgtgcgcca gccacgcctg gcagggcagt ccggctgttt cgaccagccg
     3481 cgcagcttcc gccggcggac aggcgagaac cacccgatca aagagggcgc catcgacctg
     3541 ccagcttccg gccaggcggg tcagtccctg cacgcggcga cccaggccga cttcgccgcc
     3601 acgccgcacg acccaggcca gcgccgcgtc gggcagcagc gcactgagat ccacacgcgg
     3661 cagcaacagg ttggagccgc ccgattcggc gaacagcgcg tcgcgcagca cacgcaaata
     3721 cacctggccg ctggcgcgct cggccggcgt gttcagcgcc gagacacaca gcggctcgat
     3781 cagggtggcc atcaccgtcg ggctcagtgt gcggcacagc tgcgcgacag acagctccgg
     3841 cgcgcagcga aagcccgaga tctgccagcc gacagcggca cgcagcagcg agagtttgtc
     3901 gcgccaggtc cagccgcgtg cacgcacaat gcccgccatt gcatcgagcg gagcgggcag
     3961 ccggggaaag gccaggcccg tgccgtcggg gaactgcagg gtcagtggca ggcgcagcag
     4021 cgccgtgtcg gcagcgatgc cgacgtcggc catcaggcgc agcgtttcgc tgtaggcacc
     4081 gatcaggatg tgctggccgt tgtccagtgc cagcgccctg ccgtcgggcc actggctgtc
     4141 gacacgccgc gcgcgtccgc cggggatgcg ggccgcttca aacacagtga cctgctggcc
     4201 cagccgcgtg gcttcgaccg ccgcagcgca gccggcccag ccggcgccaa tgacggccac
     4261 cttcatggtg agaatttttc cgacgggccg cccctaggaa aaatagcccc ctgggggggc
     4321 agcgcagtac gccaagcgac aagcgtgggg gccaaagttt ttacaccact tttccgaagg
     4381 cctgtgtttt ccatgccagc cagaacttgc gcagcggcgt caggctgaca cgctggtgca
     4441 gcacctggta gccgtcggcc tcgatctcgc gcagcagggt gcggtagatg ctggccatca
     4501 tcaggccggg cttttgcgcg cggcggtctg cctcgggcag cagcgccagc gcctcgtcgt
     4561 acaggccgag cgcacgctgc gtctggaact tcatcagcgc ggtaaagcgg tcggagtact
     4621 ggcgcttgag gatttcgtgc gctttcacgt cgaactgctg cagctcgttg accggcaggt
     4681 aaatgcggcc gcgcaaggcg tcttccccga cgtcgcggat gatgttggtc atctggaagg
     4741 ccagcccgag cttgtgcgcg tactcggtgg tctgcggctg cgtctgcccg aaaatccgcg
     4801 cggccacctc gccgacgatg cccgcgacca ggtggcaata ggtctgcagg cccgggaaat
     4861 cgagaaagcg gttttgcgtg aggtccatct ggcagccctc aatcaccgcc tgcaggtggc
     4921 gcgcttcgat ctggtaggtg ggggccagcg gcatcagcgc cttcatcacc ggatgggtgg
     4981 gcgagccggc gtaggacttg agcacctcgg cctgccacca ggcgagcttg gtgtgggcca
     5041 cgccggggtc ggtcacctcg tcgaccacat cgtcgatctc gcggcagaag gcataaaagg
     5101 ccgtgatggc cgcacgcctt tccctgggca aaaacaggaa ggcgtaataa aaactgctgc
     5161 cggatgcggc ggctttctgc tggacgtatt cctcggggtt c
//
LOCUS       DAIDFG010000221         5184 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_221_length_5184_cov_17.675765, whole genome shotgun sequence.
ACCESSION   DAIDFG010000221 DAIDFG010000000
VERSION     DAIDFG010000221.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_221_length_5184_cov_17.675765"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1087 a         1398 c         1481 g         1218 t
ORIGIN      
        1 ccaaggccaa actatcccgc cgtgaaggca ccgacctgtt cctgaagagc gctcgcagag
       61 ccattggtga caaggccaaa ttcgattcca agcccggtca gcacggccgc acttccggta
      121 cccggacctc ggacttcggt ctgcagcttc gtgagaagca gaaggtcaag cgcatgtacg
      181 gcgtgttgga gaagcagttc cgccgttatt tcgaagaagc tgatcgtcgc aagggaaaca
      241 ccggcgccaa tctgctgttc attctggaat ctcgtctgga caatgtggtt tatcggatgg
      301 gtttcggttc tacccgtgcc gaagcgcgtc agctggtgtc gcacaaggcg atcaccgtca
      361 atggcagctc ggtcaacatt ccgtcttaca tggttaaaac gggcgacgtg atcgctgttc
      421 gtgacaagtc caagaagcag acccgcgtgg cggaagctct gcagttggcg cagcaagtcg
      481 gcatgccggc ctgggtggat gtcagcatag acaaaggcga gggcattttc aagaaagtgc
      541 cggatcgcga cgagtttgct gcggacgtca acgaatcgtt gatcgtcgaa ctgtattcac
      601 gttaattttc gttcctgcgc ttacggggca ctgtaagcgc agggtcgctt cacccggcct
      661 tatcggtgta acgagccgag ggtattgaga ggaagaatgc atgcaaacca atttactgaa
      721 accaaaaact atcaacgttg agcagcttgc cgccaatcgc gccaaggtga ctctggagcc
      781 tttcgagcgt ggctatggtc acacgctcgg caacgccctg cgccgcgttt tgctttcgtc
      841 catggtgggt cacgccgcaa ctgaagtcac gatcgccggt gtgttgcacg agtactcgtc
      901 cattgatggc gtccaggaag acgttgtcaa tatccttctg aacctcaaag gtgtcgtttt
      961 caagctgcac aatcgcgatg aagtgacgct gagcttgcgc aaggagggcg aaggccctgt
     1021 aaccgctgcc gacattcaga cgccccatga cgtcgaaatc atcaatccag agcatgtgat
     1081 cgtcaatttg tcgcacggcg gcaagatcga catgcagatc aaggtcgaaa acggccgtgg
     1141 ttacgttccc ggcaccatgc gccgctatgg cgacgaatcc accaaatcga ttggccggat
     1201 tgtcctggat gcgtcgtttt caccagtcaa acgtgtgagc tataccgttg aaagtgcccg
     1261 cgttgagcag cgcaccgatc tcgacaagct tgtcctggaa attgagacca acggtgccgt
     1321 gaccgcggaa gacgctgtcc gtgcttcggc taaaattctt gtcgagcagc ttgctgtatt
     1381 tgcacagctt gaaggcaacg aactggctgc ttttgatgca cctgtgcagc gcagctccca
     1441 gcaatttgat ccgattttgc tgcgcccggt cgatgaactc gaattgacag ttcgttccgc
     1501 caactgcctc aaggcggaga acatctatta catcggtgac ctgattcagc gtacggagaa
     1561 cgagcttctc aagacgccaa atctgggccg caagtcgctc aatgaaatca aggaagtcct
     1621 ggcatcccgg gggctgacac ttggcatgcg cctggaaagc tggcctccgg ctggtcttga
     1681 caagcgttaa ttgaaaacag gcctctctcg ggaggcagtg cacgggtagt acctgatacg
     1741 gctatctgaa ttacaaaatg aaaggacagc accatgcgtc acggacacgg acttcgtaaa
     1801 ctcaatcgga ccagctcaca tcgactggcc atgttacgca acatgatgaa ttcccttatt
     1861 cagcatgaag ccattaaaac gaccttgccc aaggccaaag agctgcgccg cgtcgtcgag
     1921 ccgatgatca cactggcaaa agagcctacg ctcgcgaaca agcgtctcgc attcgaccgt
     1981 ctgcgtgacc gcgacatggt ggtcaagctt tttgcagagc tcggccctcg ctacaaggca
     2041 cgccctggtg gctacacccg tatcctgaaa atgggttttc gtgtgggtga caacgcaccg
     2101 atggcgttgg ttgaattggt ggaccgcccg gaaactgccg aagacattac ttctgacact
     2161 gccaagtcag aataggttat aataaataca tatcgcgcgg tggagcagcc tggtagctcg
     2221 ttgggctcat aacccaaagg tcgtaagttc aaatcttgcc cgcgcaacca atctcccggt
     2281 taatcatgcc ggtaacaaaa cgcccacatc aagtgggcgt tttgctttca catctacttg
     2341 tcagatggtg ggagtggctc cacgttgcgg gtgagaccat gtgctcagga tctgatccgg
     2401 ggtttcttca tggcagcttg gggatttcca tgagaagcag gtgttctgtg acacctagag
     2461 gcgggcaccc ttggcgaggt aacatgcgcc agtagcagtg cttttatctc ccaaccacac
     2521 aattggcgaa tctgtatggc catgtgtcgc gtaaactccc tgtacgttcc gaagctgggg
     2581 atggtgggcc acatggggct gtggtcatgg ggtcaagcca ccacactaag caggcaatgt
     2641 cgccaaaaaa gctgccaacc tgcaaaatct gacatgcaga tgtggcggca agtgcttgat
     2701 tttattacaa ttggttacgt caagtgtcaa ttcgagtttt aaatgagttg accctaaaga
     2761 tcaagaaatg agtgacatga acgtgccatt gcagaccagt cctaagagcg aggctagcca
     2821 cttggcccaa ggcttgcgcg aagacttgtt gcatcacgat cctttgctcg attgcctggt
     2881 ggaactgacg cgcatacatg gccgcccaag cacccgggca gctttgtctg ccggccttcc
     2941 cctgcccaag gcgggtcttc ctccctcgct cttctcccgt gctgcagcgc gcgcaggtct
     3001 gtcatgcaag atattgcggc gccctctcga aaaaattgat tcggcgctgt tgcctgttgt
     3061 tctgctcctt gagggagatg gggcgtgtct tttgctgggc tgggatgacc aggggcaaac
     3121 ggcaaacctg cttttcccgg aggctgggca gggcgttgtg cagttgtcac gtgaacaact
     3181 gcttggccga tatgtcggca ttgcgatctt ctcgcggccg aactttcgtt tcgatcagcg
     3241 tacgcctgag ctggctgaca ttcctcagcg ccattggttt tggggcgctt ttcgcgatca
     3301 gtttcctctt taccgggata tcctggcggc ggcgctcctc atcaatttgt ttgcgctcgc
     3361 gttgccactt ttctcgatga atgtctatga ccgtgttgta cccaacttcg cggttgagac
     3421 actctggatg ctggctttcg gcctgttgct ggtgctggtc attgattatt ttcttcgcgt
     3481 catgcggggt tattttgtgg atctcgcggg ttcgcgcata gatatcaagc tttccacgct
     3541 gatcatggaa cgggtgctcg ggatgcggct ctccgagcgg ccggcttctg tgggttccta
     3601 tgcagcgaca ctgcgctcct tcgaatcggt gcgtgatttc attgcgtcag ccacggtcac
     3661 tgctgtggtt gacctcccgt ttgccctcct ctttttcctt gttctcggct ggatttcctg
     3721 gccgctggtc attccacccc tggtaggggt cattgtggtt gtcatctaca gttacgttgt
     3781 tcagcacaaa atgcacctgc tttcagagac cacgttcagg gcctctgcaa tgcgcaatgc
     3841 cacgttggtg gagacactaa cagccatgga ggccatcaag gctcacggtg cagaaagtca
     3901 gatgcaggcc aagctcgaag acaccgccgc gtttctggcg cgtgtcagtg cccaactgcg
     3961 gttgttgtcg tcttcggtga tcaacgtcgt ctcaagcctg cagcaactga tcaatatcgt
     4021 gatgattgtg gcaggggtgt acctgattca tgaaggcaag ctgaccatgg gcggcctgat
     4081 cgcggccacg atgttgtctg ggcgcgccat ggctccgctg gggcaaattg tggctctctt
     4141 gatgcaatac cagaatgcca gaacagcctt ggcctcgctt gaggacacga tgaaaaagcc
     4201 gggtgagcgt tccgatgaaa taaattttgt acaccgtccc gatatcgccg gggaaatcgt
     4261 atttgacaat gtgcacttca gttatcccgg gcggacgcag gaggcgttgc gaggcgtgtc
     4321 cttcaggatc aagcctgggg aaaatgtggt cgtgattggc cgggtcgggt cgggtaaaac
     4381 gaccctgcag agattgatcc tgggtcttta caccccttct gaaggcgcga tcaccatgga
     4441 tggcatcgac ctccggcagc tcgatcccgc agatttgcgt cgcaacgtcg gttacgtcga
     4501 gcaggatgcc atgctttttt acggcacctt gcgcgacaac atcgcgatcg ctgctcccta
     4561 tgctgatgac ggtgccattc tggcggctgc cgaggtgggc ggtttgatcg aatttgccaa
     4621 tcgccatccc caaggcttcg acatgatcat tggcgagcga ggagagtcgc tttccggcgg
     4681 ccagcgacaa ggtgtcgcaa tcgcccgtgc agcgttgctg gaaccgcccg tgttgttgct
     4741 ggacgagccg acgggcgcca tggacttctc atcagaagca caattcaagg aacgcttgcg
     4801 cagctatgcc aaacacaaga cgatggttgt ggtgacgcat agaaatacct tgcttgatct
     4861 ggccgaccgc attattgtta tcgacgaggg ccgcatcgtt gccgatggac cctatgagca
     4921 ggttatcgag gccctgaaag ccggccgtat tgggaaagcc tcatgaagcc gcgatggata
     4981 gcgttggctc gccccttgat cgcgggcctg gagtctgtcc ggcgcatgtt gcagcctggg
     5041 cttgaccgca ccctatcctc ttggcgggag gatgatcgcc gtatggatgc agggtttggc
     5101 ggagatgcag atcgcgccat gctcgcccag gagccgcttc gagccagaat gctcgtgaat
     5161 agcctcggcg tggttttcat tctg
//
LOCUS       DAIDFG010000222         5184 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_222_length_5184_cov_16.088516, whole genome shotgun sequence.
ACCESSION   DAIDFG010000222 DAIDFG010000000
VERSION     DAIDFG010000222.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_222_length_5184_cov_16.088516"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          981 a         1635 c         1684 g          884 t
ORIGIN      
        1 aagccaccgg ccaggatgtt gctccacaca ccacgtgtaa gtttgtcgcc gcggtaggcg
       61 gccttggcgc cgtaacccgc cacgggtttg aagaagagtt gccggcgacg tgcccagagg
      121 tcgtccgcat ggtcggcggt aacgagttcg gtgtgcggaa ccacggcttg cagcaggcgc
      181 cggtgttcag ccgacacgcc ccaggaggcc agcagcgcat cgtcaccgag ggtgatgagg
      241 ttgcgcttgt ccgcatacag cgcgtgggcg cgcgggtgcg gcgtgaccac cgccgcgccg
      301 gccacatacg cgctgcgcag ggcttcatgc gttggttcgg acaggctgaa atcggtcagg
      361 cggttgtaga ccagatccac cgccaggccc gcgggcaggg cggggtgcca cagtgcgccg
      421 tctttccatt gaagctcgca cgcgtcgccc acggcggccg tgatgccgtg cgccagaaaa
      481 agctgccgtg ccaatgcaaa ttcaggtgcc aggtattggg cctccggcgc gtcgtcggtg
      541 atcaatacgc tgcgcagtgg ctgcgcgcca cgctgggctt gccactcggc gcggaacatc
      601 gccacaaagg tctggtcgat ttgcgccagg tcgctcgaaa catccatcag gccctgcatc
      661 ggttcgcagc attcgcggtg cgcacgtgcc agcgcggcgt tgagcagcgc gcccccggcg
      721 ttggtgttga tctcgatcag gcgcgggccg tcggcaccca ggtgaaagtc gtacgccatg
      781 aagacgccgg cggggccgaa attctggcgg gcaatcccgg gcgcgctggc cagtgccgcc
      841 tgctggtaag cgggcagggc gatcacgcct tcaatcgctg caatcgcctg gcccagggtc
      901 gcgcgtatgg ccgggtccag aaacaccgcc gtctcggaaa acaggtgcgg gtgtgtcgtg
      961 gccagtccgg ccaacatgcc ttgcaggctg gggtcggttt ccagctgctg gcgcaggcgc
     1021 tcggggttca gggtgcggca caggcagccc agattcaggg cctcggcgag gttcatggcg
     1081 gtgccccgtt cagcaggccg cgcaggaggc ggcgtcggag actgcgcagg cttcgccctg
     1141 gcaacagtgt ttggtcatgt aattcatgag ctccaccatg tggtcaaagt tcgcgcggta
     1201 aatcaggttg cggccgcgcg gctcgctgct gaccaggccg gaatgcgcga gttctttcag
     1261 gtgaaaagac agcgcgctgg gcgctacttc gagctgttcg gcaatcgcgc ccggtgtcag
     1321 cccgtcgggc ccggcgacca ccagcgcgcg gaaggtgcgc aggcgctgcg tctgcgccag
     1381 tgccgccagg gctttgacgg cgccggtatc atcgagaggt tttgtttcca tgtttcaaat
     1441 ataattgaat aatcgaaacg aggcaagccc aaccaggaaa acagcaatgg ccgacatcac
     1501 gatttaccac aacccccagt gcggcacctc gcgcaacacg ctggcgctga tccgcaacag
     1561 cggggtcgaa cccgaagtca tcgagtacct gcaaactccg cccagccggg aaaccctggt
     1621 cgcgctggtg gcccggaccg ggcagccgct gcgcgcgctg atccgcagca aggaaaagcg
     1681 ctacgccgaa ctccagctcg acaacccggc gctgacggac gaccagctgc tggacgccat
     1741 gctggcccac cccctcctga tcaaccggcc catcgtcgtg acgccgctgg gcacacgcct
     1801 gtgccggcct tctgaagccg tgctcgacat cctgcctgcg ccgcagcagg gcgctttcac
     1861 caaggaagac ggcgagccgg tcatcaatgc caagggagaa cgtgttgccg gcgcttgaag
     1921 accttcccaa cgtagataaa gacctgttcg cgctgcccac agccagcgca ctgcaggcct
     1981 tccggccgtc agcgcacgcg ccgcgcattt tgctgctcta cggctcggta cgcgagcgct
     2041 cctacagccg cctgctcagc gaggaggccg cgcgcctgct gcaggccatg ggcgccgaaa
     2101 ccagaacctt tgacccgagc ggcctgccgc tgcccgacga cgcgcccgac agccacccca
     2161 aggtgcagga gctgcggcaa ctcgcgcagt gggccgaagg catggtctgg acctcgcccg
     2221 agcgccacgg cgccatgacc ggcatcatga aatcacagat cgactggatc ccgctggccg
     2281 agggcgcggt acggccgacc cagggcaaaa ccctggccgt gatgcaggtc agcggcggct
     2341 cgcagtcctt caacgcggtg agccagctgc gcgtgctggg ccgctggatg cgcatgctga
     2401 ccattcccaa ccagtcctcg gtggccaagg ccttcctgga gttcgacgaa gccgggcgca
     2461 tgaagccttc cgcctattac gaccgcgtgg tcgatgtgat ggaagaactc gtgaagttca
     2521 ccttgctcac gcgtgatgtg gcgccttatc tggtggaccg gtacagcgag cgcaaggaga
     2581 gtgcggaggc actgtcgaag cgggtgaatc agcggagttt gtgaatcaaa tcggcctcca
     2641 gcccatgccg gacaagcgca ggcagctatt gaattaatag cgaccggtca gtctctcttt
     2701 gaagcagggc ctgctgctcc ctccgcttgc agaaggctgc gaaccagccg gcgcgccaca
     2761 ggcgaaacca ccagcaccgc cgggaaggca aacaaccacg ccgtgagcca ggaagtgatc
     2821 cacagtgaca gaaagcccgg ccccatcccg gcggcgcgca gcgtggatat tcctgcgacc
     2881 agcagcgaca tcaggcctga cagaaagaag ccgaacaaca tggactcaaa ttttcgggga
     2941 atcattgcgt cacctccaga ttggcattcg ctgccatgcg ctgcagtgct tcagcaacat
     3001 tcgtgccgaa catccgcgcc gtcttgatgt ccccgggtgc gaacgaatcc gcaggcgccg
     3061 aatgcccggc ctgcgccatc attcccgacc aggagccgag ccggttggcg gcttcttcat
     3121 acggcacgcc gctgtgctgc tcgggcagga tggcgttgcc gacccagacc atgccgtgtt
     3181 gcatcgcgaa gacgaagatg gacagcagcg tggattgctt gtcgcctgcc ggcagggacg
     3241 acacggtgaa ggccgatgcc agttttccct tgagggcgtg gcttttccag agccggcccg
     3301 tcgaatccat gaaggtcttg aaggtgccgg agacaccgcc caggtaggtg ggggaaccga
     3361 agacgaagcc gtcatagtcc agcaggtgtt cgggagtctt tgcgagttct tcggctttca
     3421 gcaaatgcgc atcgatgccg ggaacagatc gcacacccgc caggatgtgc ccggcaatgt
     3481 ggccggtatg gccgtgggcg ctgtgataga tgacggcaag ttgtttgttc ttcatggtgt
     3541 ggtgtccagt tcaagttgag tgaagttgag tgagtgaatc tctgaaaaaa ggcttcatcg
     3601 cgagatccag gcgatgacga cggcggccag gtacaggccg agtccaaaaa cgctgtgatt
     3661 taccaggctc ctgagccggt tttttgccgg tgccggcgtg cgcgacgacg cgatgcccgc
     3721 ccccatggcg ggctgcatga cgagccaggg cgctgcgact gtggctatgc ccacgcacag
     3781 cgccggcagc agcgtcgggc tgtgcaccca gttcatgcca aagatgccgg ccagaaggcc
     3841 cgcgaagccg atgccggttg cgtagtggac gaaccacccc aaaacgcgtt cgcctggcac
     3901 cggcgcggcc ttcgcgatcg cgtcatgcgt ccaggtgccg ttgcgccagt ggccgaccca
     3961 gcggccgatc attgcgaagt tgagcgtggg cacgcccagg cgctggagaa gcagcagcca
     4021 ggcgtccatg actgcggtgg cgcccatgcc gatgagcagg atgtgcgccg cgcctgcagt
     4081 aaatgtgttc atgtgattcc tcgttgacaa aaagtgacgg tgagggcact ctacaagttc
     4141 aagtcaactt gaagtcaaga ggttttttca tggacatcgc cgaagtcgcc aaaaagtcag
     4201 gcctgcctgc ctccgcgctg cgctactacg aagaaaaagg gctgatcacc tcggccggcc
     4261 ggcagggcct gcgccgaaca tttgcaccgg gcgtgctgga ccagctggca ctgattgcgc
     4321 tcgggcaggc cgccgggttt tcgctggacg acatcggcgc catgttctcg cccgacggtc
     4381 aggccagcat cgacaggcag tcgctcagcg ccaaggccga cgagctggac aacacgatca
     4441 agcgcctgaa ggcgatgcgc aacgggctca gacatgcggc ggtgtgtccc gcgcccagcc
     4501 acgccgagtg cccgaccttt cagcgactgc tgcgctcagc ggcggccggc gagttgacgc
     4561 cacgcgtgag gaagcccgtg cgcgtgcggt cgaaggcgtg agcgcgctgg ccaggctgga
     4621 cgcgagctgc cgcgttggtg gggcaaccag gctcatggac cgacgcttgc atgagaaaca
     4681 ggcctgcagc ccttatggat cggtcggtgt cagctattga attggtagcg aatggccgtg
     4741 cgccttgttt tgccagctgc gcgagtggct cagacctcag tcaaataaat actccacctc
     4801 acccaaccag cgctcctcga tgcgcgtgag gttggcccgc tttccaacct gcacccggtg
     4861 gcccaccgag ctgcgaaaag cggtcccctc gatttcttcc agccacctgt cggccgtctc
     4921 ctcgggaaag tccaggccaa gcgcttcagc cgcgtcaatg ccgctttcgt accctttggc
     4981 ataagcgtac agaagtgaca tcgtgtcgcg atgacggcct ggaacgcagt aaaaaacgcc
     5041 aaacgccctg tcgtaaacaa acggacggct caggtcggca agatggagcg gctccggttc
     5101 ggaggcgccg gttctctcga gggcctgcaa ttgttgagag gtcaaacttc gcatggctgt
     5161 ctggttcagg ccgcagcccg gtac
//
LOCUS       DAIDFG010000223         5179 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_223_length_5179_cov_20.394223, whole genome shotgun sequence.
ACCESSION   DAIDFG010000223 DAIDFG010000000
VERSION     DAIDFG010000223.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_223_length_5179_cov_20.394223"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          884 a         1803 c         1615 g          877 t
ORIGIN      
        1 gtccacttcg aggccgcagc ggcgcacgca cttgatgatg ttttccgccg cactttgcgc
       61 accggtcacg atgtgcacct tggcttccag ccggatgccg ctcatgccga tgggttcgcg
      121 cacgtcctgg ccgtcgatca cgaactcctg cggctcgacc agcagcaggc gctggtcggt
      181 ggagatgttg atggccttgg cggtctcgat gacgcgggcc acgtcggcct gcgtgacttc
      241 cttgtccttg accgccacca tgccgctgga gttgatgccg cggatgtggc tgccggtgat
      301 gccggtgtag acacgcgcga tcttgcagtc ggccatcagc tcggcctctt tcagcgcctg
      361 ctggatgctc tgcaccgtgg cgtcgatgtt gaccaccacg ccgcgcttga gcccgttgct
      421 gtaggcgatg cccaggccgg ccagcttgag ctcaccaccg ggcaggactt cggccaccac
      481 cgccatgacc ttgctggtcc cgatgtccag ccccaccacg agatctttgt attctttcgc
      541 catatcttta cctttttgct ttcctgtcct ctttgccgcc caacaccgtg gtggtgacgc
      601 ccctgagctt gagcgcatac ccattgctgt aacgcaggtc ggccgactcg agattgcggc
      661 cgtacctgct ggacacctgc gtcagcgtgg cggcgaatcg ttcggcacgg ttgtggatct
      721 cgtcgaggga gcccagcccc agctcgataa gcacaccgtc ctcgagcctg gcccgccagc
      781 tgccgcgggc cgtcaactcc agcagttcca gccgcgtgtc cattttttcg aacaccggag
      841 ccagcctgcg gtagcccgcc agcaccagca cagcctggcc ctgcggcccg ttgagcagcg
      901 gcaggtcgtc gacctcgacg tcgccctggt tggcctcgaa cacctcgccg taactgttga
      961 ccaggcgggc gtcgccctcc tgcccccaga aggcaatcgg ctggtgctcc tgcagcacca
     1021 cccgcagccg gttgggaaac tcgcgctgca ccaccgcctt gcgcacccag ggcacgctct
     1081 cgaacgccgc ctgtgtccgg aacagatcga cggtgaaaaa attgccggtc aacttgggca
     1141 cgacattggc gcgcagcgtg accgcgttgt tgtggcccag gtcgccgccg acccggatgg
     1201 ccgtgaggcg aaacaggttc tgctgcagca gccacgacac cccgaaggtc accagcatgc
     1261 ccgcaaacgc cagccccagc accagcgaag tggtgttcat gagcttgacg tccagcggga
     1321 caggcagggt ttggctcacg cagcgcctcc ctgcgttctc gggttgtcca ggctggccgt
     1381 gcgcagcacg tccaggcaga ggtcctcata actgatgccg gcggcgcgtg ccgacatcgg
     1441 caccagcgaa tggccggtca tgccgggcga ggtgttgatt tccagcaggt agggctggcg
     1501 ggttttgccg tcaatcatca cgtcgagacg accccagccg cggcaaccca gcaggcggta
     1561 ggccttgagc accagcgcct ggatcgccgc ttcctcgccc gccggcaggc cgcagggcac
     1621 caggtattgc gtggtgtcgg tgaagtactt gttctggtag tcgtagttgc cctcgggcgc
     1681 gacgatgcgg atcaccggca gggcccgcgc atcattcccc gtgcccagca ccgggcaggt
     1741 cacttcatcc ccgctgatga actgttcgca caacacgtcg gcatccattt gcgccgcctg
     1801 ttcgaccgcg tcgaccatgc cggaataccc tgccaccttg accacgccga tcgacgaacc
     1861 ctcgcgcgcc ggcttgacga tcagcggcag acccaggtcg tcggggaccg cgatcacctg
     1921 ccggcgctca aaggcgccgc gacgcagcaa caggtacttc ggggtcgaca ggccctcggc
     1981 cagccagatg cgcttggtca tgaccttgtc gatggcgatg ctcgacgcca tcacgccaga
     2041 gccggtgtag ggaatgccca tcagctcaag cgcgccctgc accgtgccgt cttcgccaaa
     2101 acgtccgtgt agcgcaatga agcagcgctc aaagttctcg cgtttgagct cgccgaggtc
     2161 gcgctctgcc gggtcgaagg cgtgggcatc aacgccgcgc gaacgcagcg cctccagcac
     2221 cccgcggccc gacatcaagg agacctcgcg ctcggccgag tggccgccca gcagcaccgc
     2281 caccttgcca aatggggagt ctttcaggct cacagccctt ttccttcctg cgcagaacgc
     2341 tctgtttttt gtagcaactc gaccaccttg ccgggcaccg caccaatcgt gccggccccc
     2401 atgcacagca ccacatcgcc gccccgggcg ttgtcggcaa tcgcctgcgc catggcctcg
     2461 atgtcgtcga cgaacaccgg ctcgaccttg ccggccaccc gcaacgcgcg tgccagcgaa
     2521 cggccgtcgg ccgccacaat cggcgcctcg ccggcggcgt aaacctcgga cagcagcacg
     2581 ctgtcggcct ggccgatgac tttcacaaag tcctcgaagc agtcccgcgt gcgggtgaag
     2641 cggtgcggct ggaaggccag caccaggcgc cgccccgggt aggcaccgcg cgccgccgcc
     2701 agcgtggcgg ccatctcgac cgggtggtgg ccgtagtcat caatcacggt gaaactgccg
     2761 ccggcaggcg ccggcacgtc gccgtagtgc tggaagcgcc ggcccacgcc cttgaattcg
     2821 gccagcgcct tctgcaccgc ggcgtcgggg atgttgagct ccaccgcgac cgcgatggcc
     2881 gacagcgcat tgagcacgtt gtgctcgccg ggcaggttga gcaccaccgg caggtcggca
     2941 agaatcacgc cgttgcgccg ctgcaccgtg aagtgcatct gcccgccgac cgccttcacg
     3001 ttgatggcgc ggacctgggc gtcctcgcca aaaccatagc tggtgatggg gcaggacacc
     3061 tgcggcacga tctcgcgcac ggccgcatcg tcggtgcaca aaatcgccgt gccataaaac
     3121 ggcatgcggt gcaggaaatc gacgaaggcc ttcttcaggt tgccgaagtc gtggccatag
     3181 gtctccatgt ggtcggcatc gatgttggtc accaccgcca tcaccggcaa caggttgagg
     3241 aaggaggcgt ccgactcgtc ggcctcgacc acgatgtagt cgcccgaacc cagccgggca
     3301 ttggcaccgg cactgttgag ccggccaccg atcacaaagg tcggatccag cccgccctcg
     3361 gccagcacgc tggcgaccag gctggtggtg gtggtcttgc catgcgtgcc ggcaatcgca
     3421 ataccctgct tcaggcgcat cagttcagcc agcatcagcg cacgcggcac cacgggaatg
     3481 cgtttttcgc gcgccgccag cacctcgggg ttgtcgggct tcaccgccgt cgaggtgacc
     3541 accgcatcaa cgctgaccag gttttccgcg gcatggccga caaaggtctg gatgcccagt
     3601 ccggccaggc ggcgcaaggt ggcactgcct accaggtcgg agccggagat cacgtaaccg
     3661 aggttatgca ggacctcggc aatgccggac atgccggagc cgcccaggcc gatgaagtga
     3721 atgtgtttga tcgcgtgttt catgtcgcca gctcctcgca ggcggccacc acgtcggcgg
     3781 tggcatctgt tttttgcatc tttttggcct gaagcgcccg ctgcaatagc gcaggccgct
     3841 ctgttttctg tagcatgtct gccaggaatc cgggtgtcag gtcgcgctgc tgcaccagcc
     3901 agccgccgcc gttgttcacg agaaaggcgg cgttggtggt ctggtggtcg tccaccgcag
     3961 ccggaaacgg cacgaacagc gccgcggcgc ctatcgcggc aatttcggtc acggtgctgg
     4021 caccggcccg gcagaccacc aggtcggcct cggcgaaagc acgggccgtg tcgttgatga
     4081 agggcgtgag ttcggcctgc acgccggcgg cgcggtagtt ctcgcgcagc gcctcgattt
     4141 gccgggcgcc actctggtga atgacctggg gtcgctgtgc ctcgggaatc agggccaggg
     4201 cctgcggcac gatgcggttc aacgcctgcg cgcccaggct gccaccgacc accagcaatt
     4261 tcagcgggcc gctgcggccc gcaaaccggg cggccgggtc gggctgctgc gtgaaggccg
     4321 ggcgcagcgg gttgcccacc catgtcgcat ttttcagcac attcggaaaa gccgtgaaca
     4381 cccggtcggc cacacccgcc agcaccttgt tgaccagccc ggccaccgag ttctgctcat
     4441 gcagcaccag cggcttgccc agcagcacac tcatcatgcc ggccggaaag gcaatgtagc
     4501 cgcccagccc gacgaccacg tcgggcctga cgcggcgcac gaccgcaatg ctttgccaga
     4561 aagccttgag caggcgcagc ggcagcaagg ccagcgtggc cacccccttg ccgcgcacac
     4621 ccgaaaactc gatgctctcg aagggaaaac cgcgcggcgg cacgagctgg ctctccatgc
     4681 tcggatggtc agccgttccc cggccgccca gccagtgcac gcgccagccg cgctcacgca
     4741 gcgcctcggc cacggccagc ccggggaaaa tgtggccgcc tgtgccgccg gccatgatga
     4801 gagcgcattt gctcacggcc gccctccccg catcaacggc gctggcgcgc cgcagcgctg
     4861 cgcgctgaat cgattgtggt gaaacagctt actcacgcgc ggcctccgcg catgagtaac
     4921 ctgttttcgt aatcgatgcg caggacaacc gccagcgaaa tcagattcat caaaatcgcc
     4981 gagccaccgt aactcatgag cggcagggtc agccccttgg tcggcaaggc gccgaggttc
     5041 acgcccatgt tgatgaaggc ctggaagcct atccagatgc ccacaccctg ggccaccagg
     5101 ccggcaaaca ggcggtccag cgcaatcgac tggcggccga tatgcatgat gcggcgcgtc
     5161 atccacatga aggcaccga
//
LOCUS       DAIDFG010000224         5178 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_224_length_5178_cov_17.616826, whole genome shotgun sequence.
ACCESSION   DAIDFG010000224 DAIDFG010000000
VERSION     DAIDFG010000224.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_224_length_5178_cov_17.616826"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1121 a         1612 c         1463 g          982 t
ORIGIN      
        1 cgcgcttgca ggactgggtg gatccggccg gcgaaaaagt gcaggccttt caggatggaa
       61 ccccctactc ggctgatgtg cttctgggat cgaccattac caatacaggg gatcccgctt
      121 acgatgccgc cgcgcctgcg gccgcctgga aaacctattt gttcgcgtcc ttggggcgcg
      181 gcgccaaagg catctttgct ctcgacgtga ctgacccgga tgccttgacg gaaaccaacg
      241 ccgccagcat ctttaaatgg cagttttcgg aagcggatga caacagcggt gacctgggct
      301 acaacctggc ccagatggga acgatcagcc gcgtcagcga gcaatccggg gcggtcgcaa
      361 aaatgaacaa cggcaagttc gccgtactcg tgcctaacgg gatcaacagc accaatggct
      421 cggcggccct ctacatcctg tttgccaatg gaccttacag cggtgtctgg tcgccttcga
      481 ccaattacgt caaactcgtt gctgatgttg ggcccaacaa tgggctgtcg caggcaacct
      541 ggattgacac gaacaacgat ggcgtcgccg actacattta tgcgggcgac ctgaaaggca
      601 atttgtggaa gttcgacgtg tcaagcgcca accaggccaa ctggaaggtc gcgtacgcaa
      661 acgcagatgg aacgaacaaa cgcccattgt ttgtcgcaaa gcaatcagac gcttccgcgc
      721 ttcgtctacc tatcacgtcg gcgcccgagt accgctttca tccgctgggc ggcgttgtga
      781 tcaatttcgc gaccggaaaa tccgtcgttt catcggactt tccggacggc tcgggccgga
      841 atcacgccat cttcggcatc tgggacgctc cgctatattc aagcgccgcc tatgttgcgg
      901 atccaacttt gctagacaat gcgacaaccg gcttgcctcg ccttcgtacc cagctgacag
      961 cacgaacgtt caatcggatc accgtcgaag attcggatcc gacgaacgga gttgtcggag
     1021 agggttatgt gaccggaagc gccatggact ggaccagcaa gaaggggtgg tatgtcacgc
     1081 ttccagacac ctctgaaatg gtcgtcagta atccgacgat cactcagggc ctgctcgcca
     1141 cggtctccat tgcccccgcc gcgacaagca ccgatgtgtg tttcagcggg ccagtcgcct
     1201 atgccacatt tgtggatccc atcaccggtc ttctggactc aaacgtcttc ggaacaatta
     1261 ccatcggtgg cgtcacctac caaaccgcat cgataagaat taccgaccag cgtgttacct
     1321 tctcgcgaga tgcgaccaac accgcatgtg cggcaggtca acagaattgc acccgcatca
     1381 tcggtgaaac aaccgatgaa acagcgcaat cgccaaatac ttcggcgcgg atttactggc
     1441 gtgagattcc ttccttcaag acaaggtaag acaagcatga aactcgttca aaaaaagtca
     1501 accaccggcg gcaggtgcca ccccaccaga cgcgccagcg gcggctttac gcttatcgaa
     1561 ctcatgatcg tcgttgcggt catcggaatt ctggctgctg ttgccttccc gagttaccag
     1621 gaatacatcg ccaagagcag gcgggccgaa gtccgaacca tcctgctgga agcctctcaa
     1681 tggatggagc gccactactc cgaaaatttc aggtacgacc agaacaccgc tggaacggct
     1741 gttgcagaca tctttcccgc aaacctggca cagtcgccca gggactcagg aggtgcctac
     1801 accattgcgg tatcagcgtc agctcaaaga agctacaccc tcacggcaac gcgcgttgcg
     1861 gggggacctg tggcgtctga caagtgcggc aattttcaga ttaccaacac cggagtaaaa
     1921 agcaaccccg gattctccac atcatccttc agctctgcat ctgcagcagc tacggcttgc
     1981 tggaagtagt tgtcgtccgg tgcacggaaa ccagggcctg aattgaaatt ccgtgaactg
     2041 ccggacatgc ggtggatggt cgcaatcgcg gcctccatgg ccctgcatta cggagttttt
     2101 gtcgcacaac cgctgctctc ccgtgtcggc gcgcctcaag gccaaatcca gtccggcggt
     2161 ggcggtcagc gcatcgctgc tcgcgtcatc gaacaaacac cagtgtcagt ggacagcccc
     2221 ccaatcacag ctgcaatgga aacacgtgaa atggcgcaac ctgccaagat cgaagcgggt
     2281 agtgtgcatg ctgtggccac gaagcctctg aacacctggc gcccagacgc agacatgagg
     2341 ttttatgcca ttgaagaagt tgatcaacca gccctccctg tcctgaactg gcaatttcct
     2401 tcgcaaactc ctgcctccat ccaacttcga cacctggtcg tccaagtctg gatcgcggag
     2461 aatggagata tcctgaatgt ggagttactt tcctcagaac cgcaaatcgc gggggagcag
     2521 aagcagaaca tcgaacggag cttgattcaa acctcaatga ctcctgccac gaaagatggc
     2581 cgacaggtag ccagccaacg gactctcgaa atggctctcg agttctgatt ttttccgctt
     2641 gcgtctttgc aggtacgttt tttcagctcc ggactgcaca aaattgactg acttaccttg
     2701 atcccaagca acatgacaag cgccctggag ctggccagga actccctgct cctgacctct
     2761 cccaaccccc gggttggggc gatcttcgcc tcggctgaag gtcatatcct gggcaaaggc
     2821 cacacccaac gcgctggcgg cccccacgca gaaatcgtgg ccatgcggga cgcacaagcg
     2881 cagggccact cgctgcgcag tgccaccgcc tacgtcaccc tcgagccctg ctcccaccac
     2941 gggcgcaccg ggccctgctg tgacgccctg atcgccgccg gcatcaaaaa ggtggtggca
     3001 tcgattgcgg atcccaatcc tctggtgtcc ggccagggct ttgcgcgcct gcgggccgcg
     3061 ggcattgagg tggaggtcgg cccgggtgcg gcagaatcgc gcgagctgaa catcggtttt
     3121 ttcagccgca tgattcgcaa gacgccctgg gtgcggatga aggtggcggc gtcgctcgat
     3181 ggcaagacgg cgctggacaa tggcaaaagc cagtggatca cgtccgacgc agctcgaacc
     3241 gacggccatg cgtggcgcgc acgctcctgc gccatgctca ccggcattgg caccgtgctt
     3301 gaagacaacc cgcgcctcga tgtgcggctg gtcgacacgc cgcgccagcc gcatctggtg
     3361 gtggtggaca gccggctgga gacgccgctg gatgctcatc tttttatagc tggtcgcgcg
     3421 ctctacatct acgctagtga gcaaaatgat gccaaaaaag cagcccttga agcgcgcggt
     3481 gcaaccgtcg tttatctgcc gggcaagggc ggcaaggtgg acctggcggc tatgctgcgc
     3541 gacctggcgc tgcgcgaggt caacgaagtc cacgtcgagg ccgggcacaa gctcaacggg
     3601 tcgctgatcc gtgaagacct ggtcgatgag tttctggtct acctggcccc caaactgata
     3661 ggccagggca gcggcatggc cagtttcggg ccgatcaccg agctttcgca ggcggtggac
     3721 ctcgagtttc gctcgagcga ccgcttgggc cccgatctgc gtatcgtggc cagagtcagg
     3781 ggacgcgacc tcttttagcc ctgctttatc ggcccggccc atggccgccc gcaatctctg
     3841 cgaaaatagc gccatgttta ccggaatcat caccggcgtg gggcgcatcg tcgccatcca
     3901 tagcctgggc agctctttgg accatggcaa gcgactcacc atcgaagcgc catcgggcta
     3961 cctcgacgac gtgggcctgg gcgacagcat cgcactgaac ggcgcctgca tgacagtgac
     4021 ggcgctcgac atcgccaaaa accagttcct gattgaaata tcggccgagt cgctggccaa
     4081 aaccagtggc ctgaccgaac ccggcccgat caaccttgaa aaagcgctgc gcgccaatga
     4141 ccggctgggc ggccacatcg tcgccgggca tgtcgatggc atcggcaccg tcacgcattt
     4201 cgcccaggtg ggtgaaagct gggagttgcg catcctcgcc ccgcgcgaac tggccaagta
     4261 cctggcctac aaaggctcca tcacggtcaa tggcgtcagc ctgaccgtca accgcgtcac
     4321 cgatctggat ggcggccagg ccggctgcga aatcagcatc aacctgattt cgcacacggt
     4381 tcaaaacaca gccttgggcc atctgcgacc acaatcgcag gtgaacctgg aaattgacct
     4441 gattgcgcgt tacgtggagc gcatgctcca ggacggaaag aaaaactgaa ccatgaccat
     4501 tgccatctcc cctgttgaag acatcgtggc cgacatgcgg gccggccgca tggtcatcct
     4561 ggtcgacgaa gaagaccgcg aaaatgaagg tgatctggtg ctggccgccg atcacgtgac
     4621 tccggaagcc atcaacttca tggcccgtta cggccgtgga ctgatctgcc tgacattgac
     4681 gcgcgagcgc tgcgagatgc tgcaattgcc gcccatggcc acccgcaatg gcggcaaatt
     4741 cagtaccgcc ttcacggtgt cgatcgaggc cgccgagggc gtgaccaccg gcatctccgc
     4801 ggctgaccgg gcccgcactg tgcaggccgc cgtcgccaaa aacgccaagg tcaccgacct
     4861 tgtgcaaccg ggtcacattt tccctctgca agcggtcgat ggcggcgtac tgatgcgcgc
     4921 cggccatacc gaagccggct gtgatctggc tggcatggca ggctgctcgc cggccgctgt
     4981 catttgcgag atcatgaaag acgatggcac catggcccgg ctgcccgacc tgcagatctt
     5041 tgccgccgaa catggcctga aaatcggcac catcgccgac ctgatcgaac accgcagccg
     5101 ccacgattca ctgattgaaa aaatcggctc acggacactg aataccgctt ttggcgaatt
     5161 caccgcgcat gccttcaa
//
LOCUS       DAIDFG010000225         5174 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_225_length_5174_cov_16.959172, whole genome shotgun sequence.
ACCESSION   DAIDFG010000225 DAIDFG010000000
VERSION     DAIDFG010000225.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_225_length_5174_cov_16.959172"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          913 a         1529 c         1701 g         1031 t
ORIGIN      
        1 gcccttgatg aagttgacca cgttgtcaaa cgcagagttg aaatacattt cgtagctttc
       61 ctgctccaca tagcctatat gcggcgtgca gatgcagttc tccaggcgca gcaaggcatg
      121 gccctgcagg ataggctcgg actcgaacac atcgacagct gccagtcccg gacgcccgcg
      181 gttcaggccg gcaatcagtg cttcgggttc gatcagctcg gcgcgtgagg tgttgaccag
      241 cacggacgag gttttcatct gtgccaggtc ttccagggtc accgcgccgc gtgtgtcgtc
      301 ctgcagccgg aggtgcaggc tgagcacatc gctgtcccgg aaaaactcgc ccttggaaag
      361 ggccacttca taaccgtcgg cctgggcgcg tgcgcgcgag gcctggctgc cccacaccag
      421 gacgcgcatg ccgaaagcac ggccataacc ggcaacgatc tggccgatct tgccgtagct
      481 ccagatgccc agggtcttgc ccttgaggac cgaaccgatg ccgaagttgg gcggcatcga
      541 ggcgtttttc aggccggcct gctgccaggc gccgtgcttg aggtgcgaga catagtgcgg
      601 aatccggcgc atggcggcca tgatcagggc ccaggtgagt tctgccggtg ccgtcggtga
      661 tcccacaccc tcggcgacga caatgccgcg ctcggtacag gcgccgacat cgacatggct
      721 tccgacgcgg ccggtctgcg caatcatctt gagcttgggc agtttttcaa tcagctggcg
      781 cggcaaatgt gttcgctcac ggatcagcac aattacatcg gcgtccttga gccgtaccga
      841 gagctggcct atgcctttga cggtgttggt gtagaccttg gcctggtaag cgtccagttt
      901 cgcggcacag gcgagtttgc gtaccgcatc ctggtagtcg tcaagaatca caatattcat
      961 gccgatattg tgcctgcaag ttttcccccg tgaggtggcg cgggctcaag gacggcaagc
     1021 cgccccgaaa aggaaagcag ctgtccttcc cggttgcagg ccgccgcgcg gcagcaagtg
     1081 cagcaaggtt tttcaggcgg cctggacagc ggcctctctt tgggccatgc ggtcgagctc
     1141 ggcacagaca tcggccatgc ggcccgaaat gaccatgcgg cccgcgcatg aggggctcac
     1201 gccggcctca aattcgcgga cgattttcag ccggcgcggc gacgtggcct tggccacggt
     1261 ttgacagggt ttatggtttg tcaccgccgc atggtgggcg ccggcgaccg tggttttgag
     1321 gaccactggc aggtcctccg tcgcctggcg ccgtgatgcc gcgacaaagg cgggcaaggt
     1381 gatcgctggc tgtgccggcc gcagcaaagc ggaaagcgct tgcagtggcg aaagcagtac
     1441 cgaccaaagg ggtgaaagag cgggcgaacg tgaaaaaacg gcgattccca tgaaaactcc
     1501 agaagtagag gtaaacacag gcaggatttc aaactgaaca accgccgcac cagggtgttg
     1561 gtccatgcga ccaaacgccc gccacctgtt gcgcggaaaa gaaaatgggc agtccctgca
     1621 agcgcactgc ccggaccatg gctacatcag catggtgttg cgtatcaggc ccacagccag
     1681 tccctcgatt tcgaaggcat cgccgggctc gaccacaatg gttttgaagt cagggttttc
     1741 gggcagcaac tcgatcagat tcttgttgcg gcgaaaacgc ttgacggtga cgtcatcacc
     1801 caggcgcgcc accacgatct gtccgttttt ggcttccttc gtctgtttca cggccagcag
     1861 gtctccatcc atgatgccgg catcgcgcat gctcatgccg cgcaccttga gcaggtagtc
     1921 gggctgacgc tggaacaggc tgctctcgaa atgataggtg tggtccacat gctcctgcgc
     1981 gagaatgggg ctgccagcag caacccgccc caccagaggc agggcgagtt gggccaggct
     2041 ttgcaacggc agggaaaact gtttgatgcg tgactcgttg atggagcgca gggtgtcgct
     2101 gcgcaggcgg atgccgcgcg aggtgccgct gaccagctcg atcacaccct tgcgggccag
     2161 cgcctgcagg tgttcttcgg cggcgttggc ggacttgaac ccgagttcgt tggcgatttc
     2221 ggcgcgcgtg ggcggggcgc cggtgcgggc gatggcgctt tgaatcagtt caagtatttg
     2281 ctgctggcgt gcagtcagtt tgggcatttc ggaaaaatcg ttgaagctgc tcatcacggg
     2341 gctccttgtc aaacaggggg ttccatcgtc cagaatgggg tgagaaaccc tggcaagctg
     2401 ttttaatatc cagtagctgt attttcatcc agttttttaa agaatgcaag tctcaacgct
     2461 tatgaacaga aaaatcgtga ttctcggtac cggcggcacg attgccggca ccgccgccag
     2521 tgcctcggac cataccggct acaccgctgc gcaggtgggc atcacccagc tggttgaagc
     2581 cgtgccggcc ctggccgaag gtccttgcga tctggtgacc gagcaggtgg cgcaaattga
     2641 cagcaaggac atgagttttg ccgtctgggc ccggctcgcc gcgcgggtca gtcactttct
     2701 ggcgcaaccc gatgtgcagg gcatcgtcgt gacacatggc accgacacgc tggaggaaac
     2761 ggcctggttg ctgcacgcgc tgctccatcc cggcaagcct gttgtgctga cctgtgcgat
     2821 gcgcccagcc acggcgctgg cacccgacgg cccgcagaac ctgctggacg ccgcggccgt
     2881 ggccgcgcat cccggcgctc gcggcgtgct ggccgtgtgc gcgggtgtca ttcacggcgc
     2941 gcctgacgtg cagaaggtgc atacctaccg gctcaatgcc ttcagctcgg gcgatgccgg
     3001 accgatcggt tatgtcgagg aagggcgctt gcgcttggta agaaattggc ctgaagcccc
     3061 cgttcatcaa gcgccgtcag ctatcaaaaa agtagtagaa cagcaagatg gactgggttg
     3121 gccccgcgtc gagatcgtga tgaattacgc gggcgcgggc gccggcgtgg ttcagtcgct
     3181 ggtggccagc ggtgtacaag gcctggtggt ggcggcgacg ggcaatggca cgctgcacca
     3241 tgagctcgag gcggcgttgc ttcaggcgca ggcggccggc gtgcgcgtgg ttcgcgccac
     3301 acgttgcctc gagggcaggg tgctggtcag ggccggcgac gccataaccg attcgcaggg
     3361 cctgagcccg gtgaaggctc gggtggacct gatgcttcaa ttgatgggcg gaagccgttg
     3421 acgcgatgtt tgcagccctg aaatcccacc tgtgggccta tccggcgctg gagatggtgc
     3481 atatcagcgg cattgcgctg ctgctcggca acctggtgct gctggaattg cgcgtgttcg
     3541 gcttgggtgc tgccttgccc gtgagggacc tggcacgcct gagcctgggc cttgccctca
     3601 gcggctttgc catcgcggct gcatccggcc tgctgatgtt tgcgtcccag cctgccgaat
     3661 tgctggcgaa ccgcgctttc accctgaaga tgctgctgtt attcaccgcg acgtgtaatg
     3721 cagcctggtt ccatggacgc ggctcgctcg acaaactcga tgcttgggca cgcgtgcaga
     3781 tggcggtgtc caccctgatc tggctggctg tcttggcgtg tggccgctgg attgcctatc
     3841 tctgagcaaa ccatccttca aggagctgac agatgaaacg acgtgacgtg atcggggccc
     3901 tgtgtgccgt ctccagcacc ggcctgatgc cttttgcgcg ggcgcaccac ggctggagcg
     3961 ggtttgacca ggaccggccg atctaccttg aaggcagggt gctcaagtcc ctttggcaga
     4021 acccgcatgt cgagctggaa attgaattgc cggccacact gaagctgccg gccgaccttg
     4081 cccagcgcgc gctgcctgcg cagagcgccc cggtcgatgg caaggccttg ctggccaggg
     4141 ccgttgtgcc cacgcgcaag gacaggcgct gggagatcga gctggcgccc ctgacgcgcc
     4201 tgcaggcctg gaaagtcgcc gagatcaagc ccggcacgca ggtctcggtg gtgggcttca
     4261 cctttgccgg cgagaagggg gacgcggtgt tgcgcgccga atacctgttt gtcgacggca
     4321 aggcgtatgg cctgaggtcc agtccggcct gagtgacggc gaatggcgcc gagtgacgcc
     4381 cagcaaaaag gccgtcgaca gacggccttt ttatcgtgcg gtgagagcgc tcagttcgac
     4441 agcgcttcga aagccctggc cgtgatttca tccacaccgc ccatgccgct gatcgcgcgg
     4501 tatttgggcg ctgcgtcggg tgctgccttg gcccagctgg agtagtagtc caccagtgga
     4561 cgggtttggg cgctgtagac ctcgaggcgc ttgcgcacgg tttcttcctt gtcgtcttcg
     4621 cgctggatca gcggttcgcc ggtcacgtcg tccttgccgt ccaccttggg cgggttgaac
     4681 ttgacgtggt aggtgcggcc cgaggccggg tgcgaacggc ggccgctcat gcgctcgatg
     4741 atggcctcaa agggcacgtc gatttccagc acgtagtcca gcttcacacc ggcggacttc
     4801 atggcgtcgg cctggggaat ggtgcgcggg aaaccgtcga acagaaagcc ctgggcgcag
     4861 tccggctggg cgatacgctc cttgaccagg ttgatgatca ggtcgtcgct gaccagcgcg
     4921 ccggcgtcca tgatcttttt ggcttcgatg cccaggggtg tgccggcctt gaccgcggca
     4981 cgcaacatgt cgccggtgga aatctggggg atgccgtatt tctggcagat gaaggtcgct
     5041 tgtgttccct tgccagcgcc tggcgcgccc aacaaaatca gtctcatgga tgtccttgga
     5101 tttttatgaa tcggcagcgg gcgggtggcc tgatgccggc cccggcctca cattattttg
     5161 agctgaagga tagc
//
LOCUS       DAIDFG010000226         5141 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_226_length_5141_cov_19.206449, whole genome shotgun sequence.
ACCESSION   DAIDFG010000226 DAIDFG010000000
VERSION     DAIDFG010000226.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_226_length_5141_cov_19.206449"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          860 a         1648 c         1729 g          904 t
ORIGIN      
        1 tctcttggcg aagcaagaga aagtgagtcg cccgccgggg cgagacccgg cttgccggcg
       61 tactctcggt ggtggaaaaa gatccgtcca ccacgaaaag ccgcacaata agcccatgac
      121 gctcaccgaa ctcaaataca tcgtcgccgt cgcccgcgaa aaacacttcg gcaagggcgc
      181 cgaggcctgc catgtgtcgc agccgacgct gagcgtggcg atcaagaagc tcgaggaaga
      241 gctgcaggtc aagctgttcg agcgcaacgc caatgagatc acggtgacac cgctgggcga
      301 agagatcgtg cgtcaggcgc aaagcgtgct ggagcaggcc gacagcatcc gcgagattgc
      361 caagcgcggc aaggacccgc tggccggcgc catacgcctg ggcgtgatct acaccatcgg
      421 gccctacctg ctgcccgacc tggtgcgcca ggcgattgcg ctgacgccgc agatgccgct
      481 gatgctgcag gagaacttca ccgtcaagct gctggaagag ctgcgcaccg gcgagatcga
      541 ctgcgcgatc ctggccgaac cttttcccga caccaacctg gcgatcgcgc cgctgtacga
      601 cgaacccttc atggcggccg tgccgagcaa ccacccgctg gcggcgcgca gcaacatcac
      661 cgccgaagag ctgaagaaag aaaccatgct gttgctgggc accggccact gctttcgtga
      721 ccacgtgctc gaggtctgcc ccgagttcgc ccggttttcg agcaacgccg agggcatacg
      781 caaaagtttt gaaggctcgt cgctggagac catcaaacac atggttgcag ccggcatggg
      841 tgtgacgctg gtgccgcgac tcagcgtgcc caaggaggcc ctgctgcccc cggcacctgc
      901 caaagccgtt tcgaaagcca agagcaagga agtgccggag ttgccctcct acatccgtta
      961 cctgccgttt gagggccatg tgccgacgcg gcgtgtggtg ctggcctggc gccgcagttt
     1021 cacccggtat gaagccatcg ccgccttgcg caacgcgatt tacgcctgcg agctgccggg
     1081 tgtgacgcgc ctgtcctgag cggcgcccgc gggcatttca tgatgcctat caaggcgata
     1141 cagtttgact gttgccttgg gcttgccgat tgcttagagt gatgtccaga ctcttttatc
     1201 caagtcaatc aaggacacac catggccaaa acatctccca aggcttctgc cggccgcact
     1261 ggcgcaccgc ccatcaacat cggcatcggc gaaaaagacc gcgctgcgat tgcccagggc
     1321 ctgagccgtt tgctggcgga cacctacacg ctgtacctga ccacgcacaa cttccattgg
     1381 aacgtgacgg ggccgatgtt caacaccctg catgccatgt tcatgacgca gtacaccgag
     1441 ctgtgggcgg cggtggaccc ggtggccgaa cgcatccgct cgctgggtca cccggcgccg
     1501 ggttcgtacg cgcagttcag caagctggcc tcggtgcccg acgtgcccac caccccgccc
     1561 aaggcgctgg agatggtgcg cattctggtg aaaggccatg aggcggtcgc gcgcacggcc
     1621 agggagctgt tccctgtggc cgacaaggcc gacgaccagc cgaccgccga cctgttgacg
     1681 cagcgcctga cggtgcacga acagactgcg tggatgctgc gttcgctcct cgaagaatag
     1741 gacctggcgt ccgggcgggg cagtggtgcc agaattgagg gcttgaaaaa caaattcgcc
     1801 ctctatttgc aactgatccg ctgggaccga cccgccggtt ggctgctgct gctctggccc
     1861 acgctgtcgg cgctgtgggt cgcgtcgcac ggtttccctg gctggcacct gctgacggtg
     1921 ttcaccttgg gcaccatcct gatgcgcagt gccggctgct gtctcaacga cgtcgccgac
     1981 cgcgagtttg accggcatgt caaacgcacc gcgcagcgcc cggtgacccg tggtgcggtg
     2041 tctgtacggg aagccttgat cctgggcgcc gtgctggcgc tgctggcttt tggcctcgtg
     2101 ctgaccacca acacggccac catcacctgg tcgtttgccg cgctggcggt ggccgtggtc
     2161 tacccctatg ccaagcgtta tgtgtccatg ccgcaagccg tgctgggcgt ggccttcagc
     2221 tttggcatcc ccatggcgtt tgcggcggtg cagtcgcgtg tgccggcgct ggcctggctg
     2281 ctgttgctgg gcaatctgtt ctgggtgatt gcctacgaca ccgaatacgc catggtggac
     2341 cgcgacgacg acctcaagat aggcatgaaa acctcggcga tcacgctggg ccggttcgac
     2401 gtggcggcgg tgatggcgag ttacctgatt tatctgtcaa tttgggcctg ggccctgatg
     2461 gatattgcgc agccagctat ttatttcata gcaatcgcgg tggcgctggg acaggcgctg
     2521 tggcatggct ggctgatccg ccggcgcggg cgcgacgact gtttcagggc ttttcgcctg
     2581 aaccactggc tggggttcac ggtgttcgcc ggcatcgcgc tgtcgtatgc gctggctggc
     2641 gtgtaactgc ctgccggtga tttcttcgaa taaaatcagg ctctagccct tatcgcacgg
     2701 gcgccgacag ctactctttt aatagcatta ggccgcaccg aactcgtcac cgagttccgc
     2761 ggcgcgctga cgggcggcgt gcatggcgcg catgaactgc agccgcacgc catcccgctc
     2821 cagggaggtg agggccgcgt aggtggttcc ccctttggag gtcacgcgtt cacgcaaggt
     2881 ctccggtggc tcgctggacg ctttggccag tgcggaggcg cctacaaaag tctgcaccgc
     2941 cagctggtgc gcctgctcgc ggctcagccc catctcggtg ccggcctgca ccatggcttc
     3001 gatgaaataa aacacatagg ccggaccgga gccggacagc gcggtcaccg cgtccagttg
     3061 ctcttcctgc gccagccaca gatgctcgcc ggtggtctgg atcacgcgct ctaccgtctg
     3121 gcgctcggcg gcgctgacgg ctgggcgtgc aaagagggcg gtcatgccct ggccgacgag
     3181 ggccggtgtg ttgggcatgg cgcgcacgat gcgttcggtc gccagccagg aggccatgct
     3241 gtccgagcgg atgccggccg ccacgctcag gtgcagcgcc tggccggtgt gcgggccggc
     3301 ctgcagggcg gcttcccgaa aggtctgggg cttgacggcc cagaccacca gagcggcgga
     3361 ggccagcgcc gcgccgggcg ccgcctcggc ggcgatgccg aactgcgcct ggagtttcgc
     3421 gcgctgctcg gcccagggct cgacgatctg gatccggtcg gcgggtgtgc cttgtttgag
     3481 caggccgcca atgatggcgc tggccatgtt gccgccgcct atgaaggcga tgacgctgct
     3541 gtctggggaa agggatgaag tcatgaaggg tcagtcaaaa aattcataag ccagccattg
     3601 ggtgatttgc cgcgaattga aattgtcgtc gctaatgaag tgcaggctgc ggttgccatt
     3661 gggaagccgt gggccccagc acatgccttc ggtgttgtcg aggcgggcca gcctgtggcc
     3721 ggggcctgcg ccggtgaagg cggaaaaatc ggcgagcagc ctcttggcga ccggctggta
     3781 agccccgggc tgcagcgccg ggatagccag cgtgtctgtc ccctcgcgcg tgtcgatcag
     3841 gtaaaggcgc agcgaattac ccgagagggc atcttgcccc gccatgtagg cccgctccag
     3901 caccagcagg cggtcggcgt ccagcagcag gatttcagta acgccgttgt cggcctgcgt
     3961 ggccggcggg acaggtgcgc gcggaatcgc atcggggatg taggcgatct ggcgcaggat
     4021 ttttccattg gccaggtcca gctgggtgaa gcggcacggc ccgcccggcg cctgcacggt
     4081 gggcaggggg ccgtcctggc gcagcgcgcc ttccgtggcg atccaggcat ggcggccgtc
     4141 cgggctcagg gccaggcctt ccagggtccg gttgctgcgc ggcccccgcg tgagctgaaa
     4201 gtcgaacatg gccggcaacg ccaattcgcg caccagcgtg ccatcggtgc ggctttcgcg
     4261 caaggccggt gcagctcctg tcagggcgtg cccctcgctg gtccacagca gggtctggct
     4321 ggccgggcgc cagcggatcg cttccgggtc ggcaacctgg atgcctcgca gcgccgccgg
     4381 gtaggtgctg ccatcggcct ggcgcaggct ggtcacgccc gtcaggatgg gctcacccag
     4441 ccggtctgtt tccagtgaca ggcgtgccgt gtaaaaccgc gccggattga tggccgagcc
     4501 atcatcactg agcagataga acaggccgct ggcctcgtcg tagtccacac ccgacaagcc
     4561 gccgacgatc gtgccctgaa actggagccg gtgcggcaga cgggcctcac cgatcagtcg
     4621 caggcggggt ggcgccagcg atgccagagc cggcttcggc agcctcaagc ccgccagacc
     4681 cagcgcggcg gcagcgccgt aaccgagcac ccggcgccgt gtcagttcaa acgcggctgt
     4741 ggcgggcggg ggctggctgg gcatgaagcg agtctagctc tcccgcttgt aggcgttgtg
     4801 acaggacgtg gacgtgcgcc ggcccgggct tacaaggcca cggtttgccg caccgcgtgt
     4861 tcccactgcg ccatcaggcc ggccgcgcgg tcggcgctca aggtcggcaa aaaggtgcgt
     4921 tctgcgcgcc acagggtcga gagttcagcg gtgctggcgt agacgccgct ggacagcccg
     4981 gccaggtagg ccgcgcccag cgccgtggtt tcggtcacgg ccggccgaac gacaggaatg
     5041 cccagcaggt cggcctggaa ctgcatcagg aggtcgttga tgcaggcgcc gccatccacc
     5101 cgtagttcgg ccaccggtgc ggccccggcc gccaccgcat c
//
LOCUS       DAIDFG010000227         5140 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_227_length_5140_cov_17.717601, whole genome shotgun sequence.
ACCESSION   DAIDFG010000227 DAIDFG010000000
VERSION     DAIDFG010000227.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_227_length_5140_cov_17.717601"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          930 a         1744 c         1586 g          880 t
ORIGIN      
        1 agcgccaccg cgtagccacc ggtgtcgcgc tgcacgcctt tgggcttgcc cgtggtgccg
       61 ctggtgtaga tcgtgtagct gatgtcggtg gatttcatcc actcgcaggg cacctggtca
      121 tccaggtgcc tgacgcgcag atccgaccac aactggtcac gcccggccac gagctgcatg
      181 ggcgagagcc cccggtccac cagcagcacc gcctcgggct tgtggcttga gaggcgaatt
      241 gcttcatcaa gcagcggttt gtagggcacg attttgccgc cgcgcgatcc cgcgtcggcg
      301 ccgacaatga ccctgggcgt cgcatcctcg atgcgggaag ccagcgagcc gctggcaaac
      361 ccgccgaaca ccacgcagtg aatggcacct atgcgcgcgc aagccagcat cgcaaacgcg
      421 gcctcggcaa tcatgggcat gtagatcagc acgcggtcgc ccttttgcac gcccagtgct
      481 ttcaggctgg ccgccatgcg ctgcacttcg acatgcaatt cggcaaagct gtaggttttt
      541 tcaatgccgg tttcggtcga cacgtagatc agcgccgcct ggccggcccg gtctttcagg
      601 tgccggtcca ccgcgttgtg gcacaggttg gtggtgccgc cttcaaacca gtgtgcaaac
      661 ggcggcttgt cgtagttgca gacacgcgtg aaaggctgct tccactcaat cagtctggcc
      721 tgctcggccc agaaggcgtc gggctggtct atcgactggc ggtaaaaatc ggcgtaactt
      781 gtcatgcgct gtctccagtt tttggtttca cgattcagat tttgaccacg atgcggccgc
      841 ggaccttgcc cgccatcaag tcggcggctt tttccgcagc ccggctcagc ggcacttctt
      901 ccacaatggc ttcgagtttc gccgggtcca ggtccgtcgc cagcctgtcc caagcctgct
      961 ggcggcgcgc caagggggcc atcacgctgt cgatgccggc cagcgtcacg ccacgcagga
     1021 tgaaaggcag caccgtggcc ggaaaatccg cgccctgggc cagcccgcag gccgccacca
     1081 cgccgccgta acgcgtttgc gcgcaggcat tggccagcgt gtgcgagcct actgcgtcca
     1141 ccaccgcggc ccagcgttcc ttttgcaacg gcttgcccgg tgcggccagc tcggcccggt
     1201 ccatcacggt gcccgcgccc agctgtttca gataactttc ctcactggcc ttgcccgtgg
     1261 ccgccaccac gtggtaaccg agcttgccca gcagcgccac cgtcacgctg cccacgccgc
     1321 cggtggcacc cgtgaccagc acctcgccgt cgccgggctt caggccatgc ttttccagcg
     1381 ccagcacaca cagcatggcc gtgtaacccg ccgtgccaat ggccatcgcc tgccgcgtgg
     1441 tgaaggcggc cggcaacctg accagccagt cgccctgcag gcgcgcccgc tcggccaaca
     1501 agccccagcg cgtctcgccc aggccccagc cgttgtggat gaaggggtcg ccggctttcc
     1561 agtccgggtg gctggattcg agcaccgtgc ccgcaccgtc aatacccgcc accatgggcc
     1621 agttgcgcac caccgggccc gtgttggtga tggccagccc atccttgtag ttcagggtcg
     1681 aatactcgac ctccaccagc acatcgccgg tgggcagttc ggcctcatca agggaaacaa
     1741 gcgcggcaga aaagccggcg tcggacttgt tgagttgcag ggctttgaac acggctgtct
     1801 ccggttattt ccaaactgaa ttcataatta tttataacag acttgcacgg ggctgacagg
     1861 gtctttcagg acagcccgat gacgccgttg gtatgctgga agcggccagc tccgataaaa
     1921 agcgagccca cggccgtggc gggaacagca actgggacac tcgccgtcgg aaaaagtggg
     1981 aagacccagc cgcggagccc acaccccggg ggcgggcagg gctcctcgac gacgttagaa
     2041 ctccagcgtg ccggcgatcg ccttgatgcc ggcctgagcg catacctcat caacttcgcc
     2101 gccaggggcg ccggaaacac cgatggcgcc ataaatggag ccgccgccct cgatcagaag
     2161 accaccgccc accgcaagca tgcgtgacac gccgcgaatg ccgctcatgg gcttgccggc
     2221 ctcggtctgc gccgcgagcg ccagggtgga cgtgcgaaaa ctgacggccg tccacgcctt
     2281 gttggtggca acgtccacgg tgtgcggacc cgcgaaacgg tcacgcagaa agacctgggt
     2341 caggccggca cggtcaacca cggcaacgcc gacctggtag ccctgcgtgc ggcaagcctc
     2401 catcgccgcc ctggctgctg tcagcgccgt ttccggcgtg agcgacttgg tctgaaacgt
     2461 tgcctgttgt gcaaaggcga ggccggccaa cccgaagagt acaggggcca gaaaggttga
     2521 agtacgcatg gatgtatctc ctgcagatgg ctgggttgac gaatagacgg gacttccata
     2581 ggcctggcga gccgcgcggg agagttccag cttttcatcc cccacgcagg aaggacaagt
     2641 ccaccgccat cttagcggtc ggagccgatt tggccaaagc cgcttcacca gctccgccac
     2701 cgggagtcag cgtgtcactt gtgcgcgatg ggcttgtccg tgaagagata gtccttcatc
     2761 tcactggaaa acttcggatc cttcctgcgg atccactcaa gcaccatggc cgcgtgttcc
     2821 ttttcctcgt cacggttgtg ctgcaggatg gccttgaggt cggggtcctt gcaggcatcg
     2881 gcgcgctggt tgtaccagtc caccgcttcc agctcttcca tgagcgagac gatggcgcga
     2941 tgcatgtccc gcgtttcgtc ggacagttcg ttgacgggtt cgtggtaacc aacactggac
     3001 atggcgtttt ctcctgttgc caagacttct tatagcagac cccggccggc ggacggtgat
     3061 caggggtcgg gcccggcagc ttcgccgggc gagtccggcg cgccctactt caccagcgcc
     3121 agcatgtccc tgaacgccgg atgctccgcg gtgcgcagcc actcgaaggc caccatctcg
     3181 gtcgtcacca gttcggcacc ggcgccggcc aggcggtcga aggcggcgtc gcggttgcgt
     3241 tcagtgcgcg agctgcaggc gtcggtgacg acccagacct cgaactcttc ttcgagcagg
     3301 cccagtgcgg tctgcagcag gcacacatgc gcctcgcagc cggccatcac gatgacgccc
     3361 cgcccttctt cttccgctgg cgcggctttc tgcagatgtt tgggcaggct gcgggcgttg
     3421 cctccagcgg gggccttgcg cgcgggcggc cgcaaccact cggccaggcc gtcgtccacg
     3481 gcactgaaat gcatcttgtc cagcgtccgg ccgccggccg ccgcgatggc ggcagcgacc
     3541 ccgggcacgt tgggccccag cttgtcgggc gtctgcgccg tgccccacac cggcacggcc
     3601 atcagcctgg ccatgccggc caggcgcagc gcattggcaa ggacaagctc accttcaaaa
     3661 atggcgggca tcagacgaac ctggtagtcc accagcacga gttgggagtc atcggcatca
     3721 agcaacatgg ttattcctga aggttttgtt caaagagggc tttccgacaa atgcggcaag
     3781 agcagcaggg tctcttcgcc gcaagccagg caatgtcagg gcgtcggcgg ttgttgcggc
     3841 agttccgcaa acttgagcag gcgcgtcgcc agcagggtct cggccaggaa acacagcagt
     3901 gcgaacagga agcaggccac gcccgagaga aaacacacgg tggcgatctt gagcgtgggc
     3961 aggtcgctgg tctcgccgag aaagagcagc acaatggtca ggccgatcag gatggcgcaa
     4021 aacgtcagca gggccaggca gccgttgacc agccagccgc ggtgccgcag ctcgccgagt
     4081 tcggcgtaca tcctggtggc ccgggtctcc tcgaccccac ccgcttccag ccggttttcc
     4141 agaaaacgcg cacggtcgat gatgcgggcg aggcggccgg ccacggcggc gatcatgccg
     4201 gaaacggccg tgagcaggaa cacgggggcc acagcgagct gtatgccgtg ggtgaccgtc
     4261 gcaaggtcgg gggcaaaatt catccagggc tccgtcagca tcagggggac gcccgtattc
     4321 tgtcatgcaa aacgccgccg gcagcccgcg cgatagcagc cttcctacgc aacagtcgtc
     4381 actgtccgac agcacgcccc ggcagcgtcc atcacactga cagccatacc gctgcaaaat
     4441 gcctcgccaa gccaaggaaa tctgcatgtt gccccacggc aggacaacat gtccccgaag
     4501 ccacgttcag gaccagccgc cgccggtgct agcatcatca cgtacccctt tcgaccctga
     4561 ttgaagacgc atgaaactcc ccgacaccct taccgagagc gttaccgaga gcgcttccac
     4621 cccagctcca gctccggccc tcctgcggga agaggctgcc cgctacgccc tgctgcgccg
     4681 catcgcgccg gccatccgcc accacatggc cggcacactg caacccatcg gcatgattgc
     4741 cgccattctc gaccggcggc tgcaggcaga gacacccgac ctggcggtgc tgcgcgacaa
     4801 cagcaagtcg atcagcacgc tgtcgcgctc ggcatccact gccagcatga acctgatcac
     4861 ctgggtcgtc cccaaggaaa acggcagcat cgcgctggag gccggcatcg aggaagcgct
     4921 gggcatgctg gcgaccgacc tggcttttcg cggcttcacg gtgaccaacc aggtcaatgc
     4981 gcccgaggtg accacctctg tgtttgcgct gcgcaccgtg ttcaccgcct gcctgattgc
     5041 gctgaccgac gcggcgcagg cccccgccag cgtggtgctc agcagcgaac ccgccggtga
     5101 ccagacccgg gtctgcatca tggtcacccg caccgagggc
//
LOCUS       DAIDFG010000228         5119 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_228_length_5119_cov_17.090837, whole genome shotgun sequence.
ACCESSION   DAIDFG010000228 DAIDFG010000000
VERSION     DAIDFG010000228.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_228_length_5119_cov_17.090837"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          904 a         1524 c         1690 g         1001 t
ORIGIN      
        1 caggcgcagc ggccccctcg gggggcagga agcgacacgt agtgagcgaa cgtgggggcc
       61 atgtttttcc gtagggccgc cccgaggaaa aatgcacccc ctcggggggc agcgcattag
      121 acgaagtgaa aagcgtgggg gcttggccta ttcagcccga gtctgcgtga tgtccgctgc
      181 ggcctgcagc tgttgcttca catttcccgc gagtttgcgg aacaggggtt gcgctgccgc
      241 cgacagggga acggtttcgt tctgctgggc aagggtcatc gggtcctggt gggcgccgtt
      301 gacacggaac tcgaagtgca ggtgcggccc ggtggcccag ccggtcgcgc cgacggcgcc
      361 gacgttctgt ccctggctca cgctctggcc tctttgtacg ttgatacggc tcagatgggc
      421 gtaaacggtg gtgtggttgt tgcggtgttt gatgaacacg acgttaccaa acccgttttg
      481 cacgccggca aactcgacca cgccgtcgcc gatgctgcgc acggccgtgc ctgttggtgc
      541 ggcgtaatcg acaccgaggt gggcgcgcca tgtctgcagg atgggatgaa aacgcatctt
      601 gaagccgctg ctgacacgcg agaactccat gggcgaggcc aggtaggcgc ggcgcaggct
      661 ctggccgtcg agcgtgtagt agccaccttt ggctgcgctg gcgccgcccg cctggtcgcg
      721 gaaccacatg gcctgcagtg tcttgccgtt gttgacaaat tcggtgctga ccacccggcc
      781 ggtgcgcagc gcttccccgt cggcttccag cgtttcgtaa accacgctga aacggtcgcc
      841 tttgcgcagg gcgcggtgga agtcgatatc gcccgagaaa atttcggcga cctggatcgc
      901 cacggcgtca gggatgcccg cattgtcggt ggcggcaaac agcgaggtct ggatggtgcc
      961 gctggccaac tgggtcgagc tggtgtaggc cgcagtttcc acgcgggacg taaaacggtt
     1021 gtctgccgca cgttcgacca ccagccgggt gaatttttct tcgtcgtcgg tggcccagcg
     1081 cacggtcagc ttgagcaact gattgttgtc tgtcgcttca accgtgacat tcttgccggg
     1141 gcggcccatc agggtcaggc gaccgttggc gtcgctgcgc aaaaaggcag cggctgcagc
     1201 gtcgtcaatg ttcaggcgct tgagcagggc gtcggccgtg tcggtgctgc gcgtggtttc
     1261 gctgcgaaac agcttgaact ggaaattgtc cagggcttcg gtctgtgcct cggtgggcag
     1321 gctctggacg gcttccagaa cctggcggac cggcaggtcg gcggcatcgg gtgcgagcgg
     1381 tgcaacgccg aaagcggtga ccccggttcc cagcagcaag gcggccagcg agcctgtgat
     1441 tcttttagga tggcggcgaa gcaggttggt gactgcttgc agcgcgtacg cgagatcggt
     1501 tttgatcaaa actggtgttc ccaacctttg taggcctcag gctgcagaag cagcctgata
     1561 gcgactgatg atggtgttgc gcactcccct gcaaaaggcg gacggtgcaa caacttagaa
     1621 tcggtttgca atttgcgggc caagctgctt tggggccccg caacggcaga aacgcgatta
     1681 tatctgtcag acaactgccc gacctgtgaa aaacccttat gaatcaagag cttgttacaa
     1741 actttcccgt gacggaccgt gccagggagg ccttggccgt atccctgcgg ggctgtgaag
     1801 aactgattcc ccaggacgaa tggctgaaaa aactcgcgcg ttcagaggct accggcgttc
     1861 cgctgcgcat caaattgggg ctggacccga cggcgcctga cattcatgtc ggacataccg
     1921 tggtgctgaa caaaatgcgt cagctccagg atctgggcca tacagtcatc tttctcatcg
     1981 gcgacttcac cagcctgatc ggcgatccgt cgggccgcaa caacacgcgt cctccgctga
     2041 cccctgagca gatcaaggcc aacgccgaca cctactaccg ccaggcttcc ctggtgctgg
     2101 acccggcccg gaccgagatc cgctacaaca gcgagtggag cgacccgctg ggcgcgcgcg
     2161 gcatgatcga gctggctgcc aaatacacgg tggcgcgcat gatggagcgc aacgatttcc
     2221 atgaccgttt caaggccggc acgccgatca gcgtgcatga gttcctctac ccgctgatgc
     2281 aggggtacga ctcggtggcg ctcaagtccg acctggagct cggcggcacc gaccagaagt
     2341 tcaacctgct gatgggccgc cacctgcagg tcgaatacgg tcaggagccg caatgcattt
     2401 tgaccatgcc gctgctcgaa gggctggacg gcgtggagaa gatgtccaag agcaagaaca
     2461 actacatcgg catttccgaa gacgccaaca ccatgtttgc caaggtgctg tcgatctctg
     2521 acgtgctgat gtggaagtgg ttcaccctgc tgagcttcaa gaccgaggcc gagatcgccg
     2581 cgctgaagat agaaatcgaa ggcgggcgca acccgaaaga cgccaaggtg atgcttgcga
     2641 aggagatcac ggcccgtttc cattccgccg ccgcggcaga cgccgccgag caggacttca
     2701 tcaaccgcag caaggggggc gtgcccgacg agatccccga aatcgcgttg agcggcgcgc
     2761 cgctgggcat ggctgcactg ctcaagatgg cggggctggc cgcctccacc ggtgaaggca
     2821 accgcctgat cgacggtggc ggtgtgcgtg tggactccag cgtggtcagc gacaaggcgc
     2881 tcaagctgaa tgccggcatc tatgtcgtgc aggtgggcaa acgcaagttc gcgcgtgtga
     2941 cgctttcctg aatcgttggc tggccatgaa gctgccgaca ctgcaatccc tgacgccgca
     3001 gcgcctgctg atggcttcgg tggtggtggc gcttgtcacc atcgtgctga aaaccggggc
     3061 gtggtacatc accgactcgg tgggtttgct ggccgacgcc atggagtcct tcgtcaatct
     3121 ggccagcgcg atcttcgggc ttaccatggt cacgattgcg cagcggcctg ctgacgagga
     3181 ccacccctat ggccaccaca aggccgaata tttctcttcc gggtttgagg gggtgctgat
     3241 catcgccgcg gcactggcca tcatctgggc ggcgggccac cggatgtttg acccgcggcc
     3301 cctgcaacag gtgggttggg gtctggcact gtccgtggcc agctcgggtc tcaacggttt
     3361 gctggcctgg atcatgttcc gctccgccag ggcgcaccgc tcgattgcac tcgaagccga
     3421 tgccaggcac ctggtcaccg acgtctggac ctcggcgggc gtggtggtgg gcattgttgc
     3481 cgtcacgctg acgggctggc tgtggctcga tgcgctggtg gcgatcgggg tggcgctgaa
     3541 cattctctgg gaaggtgttc acctgatgtg gcgctcgtcg caggggctga tggacgaggc
     3601 ggtggagccc gaggtgctgg ccgacattca caagaccctg gaagagttcc agcaccacac
     3661 catccgcttc gatcacatca gcacacgccg ctcgggccag cgccgttttg tggacatgca
     3721 catgcacatg ccggccagct ggtcactcgg gcgcgccgct gcggttcgca ccagcgtcga
     3781 gcaggcgctg atgagcgccg tgccgggcct gcgcgccacc attcagttgc tgccttccga
     3841 tgtggagacg cactttgact atgaaaagga cctgatttga tcagcgtgat acagcgcgtc
     3901 agccaggcgc gtgtggtggt ggacggtgaa gtggttggcc agatcagcgc cggcttgctg
     3961 gtgctgctgt gcgccgagcg cggcgacagc gatgcccagg ccgacaagct gctggcaaaa
     4021 atcctcaagc tgcgtatttt ttcagacgag gccggcaaga tgaaccgcag cgtgcaggac
     4081 gtggccggcg gcctgcttgt cgtcagccag ttcacactgg ccgccgatgt gtcgggcggc
     4141 aaccggccca gcttcaccgg tgcagcggcg ccggatgagg ggcgccggct gtataactac
     4201 tttgtagctc aggcacggct ggcgcatccg caggtagaga ccggccagtt tgccgcggac
     4261 atgcaggtcc acctggtcaa tgacgggccg gtgacgattc ccttgcgcat ggatccgcta
     4321 tcgatttaat agctgttggc gaccgcccga taagggctga tggccaattt gatacatgaa
     4381 aactcagcgg taagggtttt tgacgcccag gccggcgagg atttcaatct cgcgcgcttc
     4441 catggcctgc gcatcagcct cacccgtctc atggtcccag ccctgcgcat gcagcacgcc
     4501 gtgcaccagc aggtgcgcat agtgcgcctg taatgttttc ctgttctcgc gcgcctcttt
     4561 ggctatcacc ggcgcacaca gcaccaggtc ggccgtgacc agcggtgcca gggtgtagtc
     4621 gaaggtcagc acgttggtgg cgtagtcttt ttgccggtag ccgcggttca gcgcctggcc
     4681 ttcttcggcg tccacgatac gcacggtgat ctcggcatcg ctgtccagcg catggcgtat
     4741 ccagcgcgcc acgctgtggc gcggcagggc ggcgcggtgg cgtttggcgt ccttgatgtc
     4801 gccgaactgc agcgacaggc tcagctgttt aagagccatt tcggcttttt gtccttgtgg
     4861 ggcgggcgac aggcactatc gaatcgatag cgtcggctat gcccggggtt ttgcccttgt
     4921 agccgtcata ggcgtccacg atgcgcgcca ccagcgggtg ccgcaccacg tcggcgctgg
     4981 tcaggcgggt gatggcaatg cccttgacgc gcttgaggac acgttcggca tcaatcaagc
     5041 cactgagctg ggtgcgcggc aaatcgacct ggctgacgtc gccggtgatc accgccttgg
     5101 cgccaaagcc gatgcgcgt
//
LOCUS       DAIDFG010000229         5083 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_229_length_5083_cov_17.250994, whole genome shotgun sequence.
ACCESSION   DAIDFG010000229 DAIDFG010000000
VERSION     DAIDFG010000229.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5083)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_229_length_5083_cov_17.250994"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          899 a         1710 c         1620 g          854 t
ORIGIN      
        1 gctcggcacg gcggaagcgg taaatgcttt gtttggggtc acccaccagg aacacgctgg
       61 gtgcggtgcc ggcacccgag tagccgatga gccaggcgta cagcgcctgc cactgcagcg
      121 ggttggtgtc ctggaactcg tcgatcatca aatgccggat gcgcgcgtcc agccgctcct
      181 gcacccagcc cgacagcacc gggtcggcca gcatcagcag cgcggcgcgt tcaacatcgt
      241 tcatgtcgac ccagccgcgg tcgcgcttca agccggtgaa ctgggcaatc agcacacgcg
      301 tcaggcgcgc catgcgctgc tggtagagcc aggcgtcgtg ctgctggcgg gccgccacca
      361 cgcgcagcac cagatcctgg gccatgcgta cctgttcgat gccgacgatt ttttcgccga
      421 acttgcgggg cgtgcccttg tcggtcagca gggccgcgat ggccgccggg aagtcgccgg
      481 ccgtcagcgc acgctccagc tccaccccct tggccgaaaa agtcggcgcg ctcgcgcggc
      541 ccagcgccac ggcggcgtca ctgagggttt gccggtgaaa gcggttggtg ctgagcgact
      601 cttccggttc atcaaggccg ccaaattcgg tgaactgtgc gctgaaggct tgcaccgagg
      661 tgtcgatcac gcctttttca tcggccagcg tgaactcggt gcgcttgtcg agtgcggcct
      721 gcagcgcctt gtcggtctgg aagcgcccat aggccagcac caccgcctca aagtcggcct
      781 tgagcgcggc atcagccgaa acggctgcgt aaaagcgccg ccacaccagc gcgcgcgccg
      841 gcgcgtcgtc ttccagcagc tcgtagttca ccggcagctc caggcgctgc agcaccgcca
      901 ccggcgcgct gcgcagcagg gccgcaaacc agctgtggaa ggtgcggatc tgcaccgagc
      961 ggccgctggc cagaatggat cggtataaat ttgatagctg ctcgcgcacg gcctgtgcgg
     1021 actgcaggcc tttttggctt gaaaaatcag cctggacgcc gcgcatctcc agttcgcgct
     1081 gaagggtggc gtcatcggca tgggcaaaag ccttgagcca ttcgtccagc cgctcgcgca
     1141 tttcgccggc ggcttttttg gtgaaggtga tggccagaat ctcgtgcggc agcacctgct
     1201 gccccggtgc agccgtggcc ccgtcaagca aggcgcgcac gatgcgcgac accagcatcc
     1261 aggtcttgcc cgcaccggcg caggcctcca ccgccacgct gcgccgcggg tcgcaggcga
     1321 tggcgtagaa cgcatcagcg gagaccggcg cgccgttgca ctcgtaggcg gcggtgtggg
     1381 gaatcagcga tggggttgaa gcgctcatac gccctctcct gccgtggatt ccccgtccag
     1441 actccagaag tctttacgac agagtcctcg ggcagcgcag taatcgcagg ccttgccttc
     1501 gcccagcgcc ggcagtttgg caccgcgttc aatacgtgcc atgtcggtca ggatgctgtc
     1561 gatgagttcg tcgcgcaggc cgacgatgtc gggctgctca taggttttgg tgggttcctt
     1621 ctcgcccagg ttgacgtagg ccgctgccag cgtgtcgtcg ctcatcaagg cggcataaaa
     1681 cgccagttgg gtgtcttcct ggccgttctt gatgcgctcg ctcgtggtgg tgcgcggctc
     1741 ggtcttgtag tcaatcacca gagcatgccc gtcgggctgg cgatcgatgc ggtcaagttt
     1801 gccgagcagc gtgagcgtgc cgacgggcac ctcgcgccag acttcggctt catcgaacac
     1861 cgcgccggtg gcttcgtggt cggccagcca tttcaaatag ccctcgcgta cgccgggcca
     1921 ggccgcggca aagggcaaaa actcgctgtc gctgagtgcc agctcctgcg tggcgcgctc
     1981 ggccgctata tttatcatag ctgtccgcgc tgatgcatcg gtcgttggcg ccgccttgag
     2041 tgcttcatgg aaatgccgca gcaggctgtg cagccagttg ccgaagtcgc gcttgcccag
     2101 ctcgctttcg agttcttcag gctcctgcag ccgcagctgg cgcagcgcaa aaaagcggta
     2161 agggcaacgc cgcaggtctt cataggcgct ggccgaaaga cggttgacgg gcaaggcctc
     2221 gccggtcgga aagggatgga gcgtgggctg ggtggccacg gcacgcaggc tgcgagggtc
     2281 ggtggccatg ccctgtgctg cgtcccggtc cagcagcagc tcctgcacaa agccgctggg
     2341 gatcaggtgt tcgcccgact cactggtacg ccaaagcaca tcgagatgcg gcatgtgcag
     2401 ggcatagtgc caggcctggc gctggccggc ggcaagcact tcccgcgacg gcaggcccag
     2461 caactcgcgc tggccgggcg tccaggggcc ggcgggttcc ggcgacaccg gcaggcggat
     2521 ttcatcgcag cccggcagca ccacggcctg catgggccgc ccaagcagtt gctgcagcgg
     2581 caggatgacc acttgttctg cgggcggatg cacaggggaa aatttgccgc cttccagcgc
     2641 ctggttggcc cactgcgtga agtcatgcag ccgcatcacc ggcgatgctt caaattcggt
     2701 ttcggcgcct tcatgcaggc gcagcacatc cagcaccgtt tgcccggcct cgtcctgcgc
     2761 caacgcatcc cactgccccg ccgattgcaa aagggtgcgc aggtcgcgca gccatatcgc
     2821 cagagggcgg gcccggccga atgggctgcg caacgcgttc acttcgcgcg ccaacggccc
     2881 cgttgccgca aaggcggtcg gcaggaggcc ttcttccgga aaatccggca ggtcgcgcca
     2941 ggcgcgcaca ccggtcttgc gcagttcggt ttcggcagcc gtgaccaggg cggcgtcaaa
     3001 tgccgctgcg tttttcaacc agtccagcac ggcgtccgtc gatgcatccc agggcatggc
     3061 gcgcagcagg ctcatcaggc tggcggcagc ccgggtggtc gagagcttcc agccggtttc
     3121 gtcacgcatg gcgataccgc gctctgccag catggcgccg acacggcgcg tgagttgccg
     3181 gtcctgcgca atcaacgcca ccgggctgcg gccttgcgcc aggtgcgcca gcacgcaggc
     3241 ggcagcccgg ccggcttcgt cttctgcgtc ctgcgcaata tgcagcgcgg ggggtgccga
     3301 ggactcttcc agggaaaggc agagcgggat ggaaaccgaa cgttcgccga aatgggcctc
     3361 gagggcttca atgagcgggt cggcctgaaa gccttccagc acggcaaaca gctgcgggtt
     3421 cgcctggaaa aggcggtctg tgggatagga agaagccgcc gcccaagcca gcgctatgcg
     3481 ccccacggcc gcctcgagtg ccagcaccgg cgaatccagg cccgcaccca gcgtcagcgc
     3541 gagccgctcg ctccaggccc ggcgcccggc cggcacgacg gcagcggcca caccagccag
     3601 gctccaggcc gcctcgacca gccggccggc cagtgcgtcc tgctgggcgc ccaggcccgc
     3661 acggcccagc agcgatgcgg ctgtgagcat gtcgaccgcc acatccatgc gcacatcgtc
     3721 gcccgaaggg acatgcaggc ccagcgtgcc gcccaggccg ctggcccaat tcatggtggt
     3781 ctcaaagcgg ggaacaaaaa aagtctcccc cgcctgcgca gcccaggccc ggcgtgcctg
     3841 ctggatcagc tgggcatagg gcagcaacac cacgacctct gccgggtgga cctcccgcgc
     3901 ccgcaccacg gctgccagct ggcccatcgc ctgctgccag cacagcgcat caggcgtagg
     3961 caaggcggcg tcggcggtcg gggcgagtgg catggcaggg ttggagatag gctggtcgca
     4021 ggaaagaaat gggcaaagtc cgtgaaatga agcgggcgcg gtggccaaac gccgcaaatt
     4081 gaataatcgc tatcggagcc atagccccgg cgtgtccgac ccggccactt gctttctgcc
     4141 acaatgactg caactttagt ccccaacgta tcgtaaccat cctaaccaga caaaggagcc
     4201 gccatggcga gcgaactgat caaacatacc accgatgcca ccttcgaagc cgacgtgctg
     4261 caggcctcca aacccgtgct ggtcgactac tgggccgagt ggtgcggccc ctgcaagatg
     4321 attgccccga ttctggacga agtcgccacc ggttacgacg gccgcctgca gatcgccaag
     4381 atgaatgtgg acgaaaaccg cgacattccc gccaagttcg gcattcgcgg catcccgacg
     4441 ctgatgctgt tcaaggacgg ccagctggcc gccaccaagg tcggcgccat gagcaaggcg
     4501 caactgaccg ccttcatcga ccagcaactg gtctgatcgg ccttgtcccc agccaccggc
     4561 gcgctgtccc tgcacagcct gccggtgcgc catccggcat gaagcggatc ggcaggacct
     4621 gctcccgcac ctccatcgcc ccgataacca cccccaattt cctgtcataa tggccctgtt
     4681 cgctgaccgg ctgctgccgg ccggttcgag ttcccggttt gtgaagcgcc tctagcttcc
     4741 tttcagacct cccccgtttt ttatctgaac tccgcgagga gttgacttca cgcaaggttt
     4801 ccccatgcac ttaaacgaac tcaaggcact gcacgtgtct gaagtcctca agcaggccga
     4861 agagcttgag atcgaaaaca ccggccgcat gcgcaagcag gagctgatgt tcgcgatcat
     4921 caagaagcgc tccaaaacgg gtgaaaccat cgttgccgac ggcgtgctcg aagtgctgcc
     4981 cgacggcttc ggcttcctgc gtgcgccgga ctccagctac accgcctcca ccgacgacat
     5041 ctacatcagc ccgagccaga tccgccgctt caacctgcac acc
//
LOCUS       DAIDFG010000230         5082 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_230_length_5082_cov_16.447583, whole genome shotgun sequence.
ACCESSION   DAIDFG010000230 DAIDFG010000000
VERSION     DAIDFG010000230.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_230_length_5082_cov_16.447583"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          788 a         1673 c         1705 g          916 t
ORIGIN      
        1 cacaacgcct actttgcggc gatccagtcc aagccaggtt gccgcgcgat ctcgaccgac
       61 acctgcgtgc cgatctcgcg cctggccgac tgcctgctcg actcggtcag cgaagccgat
      121 gccagcggca tcccctactt cctggtgggc catgtgggcg acggcaactt ccacttcggc
      181 tacctgatcg atccggacaa accggaggaa cgcgtggccg ccgaagcgct gaaccacacg
      241 ctggtggcac gcgccctgcg cctggaaggc acctgcaccg gcgaacacgg cgtcggtctg
      301 cacaagatgg actttctggt gaccgaggcc ggcaacggcg cggtggacat gatgcgcacc
      361 atcaagcgcg cactggaccc gcacaacatc atgaacccgg gcaagatctt cacgctgtga
      421 aagccgcggt ggcctgacag cgtcaccgtg cctgcgacgc gccctccagg caacctcaag
      481 tcgctattaa attcatagct gcgtgcgccc gatcaccggg ggccggatgc cattccggct
      541 tgaaataaat ggcccgtcgc ggtaatctag ggtctcctca tgtttccaac acgggagccc
      601 gcatgaaaac cctgtccgcc ctttccctgc ttcttgccgc tggcctgctg gccggttgtg
      661 ccgcgccccg gcagcccctg gtcaactacg gccatccggc gctgaaccag gtggcggccc
      721 aacaggccgc gcccgcactt cagaacgatg cgggtcgcgt tgcgaccggt gcggtgatcg
      781 gcggcctggt gggcagccgt ttcggccagg gcaacgggca ggtcgctggt gccgccatcg
      841 gtgcggcagc gggcgccatg gcggcacagg gcggctacag caacgaggcg gccggcggcg
      901 cgctgatcgg cgggctggtg ggctcgcgtt ttggcggcgg caacggccgc actgccgcgg
      961 cggccctggg tgccgggctg ggcgcctggc tcgccgtgcc gcgggaataa ggaacaggaa
     1021 cagaaatcaa gcctccccgc ccgaaaaaaa ggccagcgac gctggccttt tttgatgatt
     1081 tcaacccttc aaccggcaaa ggccgcttcc acttcgcggc ggaacaccgg gtcgcgcggg
     1141 tgggcaccgg gctccatcgg accggcctca ccaagcagtg acagcggcgc cagcgagcgg
     1201 ccccagtaga gctggcacga cggggtattg accagccgcg tggcggcgcc ctcggtggtg
     1261 ctgccgccgc tgtcgagcgc caccttcagg gtgcccgccg tggatttctg cgctccggtg
     1321 cccagccaga ccgtcgccgg cagttgctcc cactcgcgga tgtcggcctg ggcctgcgtg
     1381 gcattggcca cgccctgcgc caccaggccg gcgaacagcc cgatcatgcc gatgccagcg
     1441 gcgtcgcggt tgccggtgct cgccgccgca agcagcccga tatcacctac cgcactggcc
     1501 accgtcgcca cgccttgcgc gccctccttg aacgaggcct tgccgttgag cacaccgtcg
     1561 atgggccggc cgccgcgcgt ggtcgcctgg aagccgaggt cggcacccat cacaaagctg
     1621 ccgacggcgc agtccgaacc gccggcggcg gtgcaggcgt tttttgcggt ctgcaccggc
     1681 agctcatggg actgccactt gaggatctcg cccgacttgc cgctgccgta tttgtagggc
     1741 gagcggccgc tttcaaacag caccagcacc ggctggtcga cacgaaccgt gctgtagttg
     1801 ctgtcgccct tgatggcctg ctgcaggaag tcctgggcca tgccggtgtt gccgtcgcag
     1861 tagctggccc agccggccat gaaaggcatc aggccgaaat cgccggcgta ctgctcggct
     1921 tcggccaccg tgtcctggta gtcggcctgc ttgaacaggg cccgggcgtt ctggaagtcg
     1981 cccctggccg catacaccag gccgcggtag aaatgcgcca tggcgcgttc ataaggctcg
     2041 cccttgtagt ccttgatttt ctcggccgtc cagagcgact tggctttttg cgcctcggga
     2101 ttgtcggcgt aaatcatctc gatgcgggtg acggcggcgt cgaacgcctt ttccgccagt
     2161 tcgatgtcgc ccagcgacag ggccgccatg ccgatgcgct ggaagttcag cgtggcgttg
     2221 cgctcgcctt cgatgtacag gcgctggaac agcggccgca gttcggcgct gggcattttt
     2281 tcggcaaaca cactggccgc ggccagtgtg tcgggcggca ccggcgcggc cggcgcccag
     2341 cgcttggcgg gtttggcgcc cccttgggcg gcggcctgcg ccgccgcctg ttgctgggcc
     2401 ggctgcagcg gtgcctgcat caggctctgg aacatgccgc caaactgggc gtgcgccgga
     2461 ggtaccagtg ccgccgccag cagcgcggca gcggcagcgc cacggtgggt cctgaagaac
     2521 gaacgcttca tcatgaaact cccctgcggg tgcggttatc gggccaatgc ctcgccaagc
     2581 cgggccaggg tctgttcggc caccagcttg ccggtgtcgc ccacggtgac gggtttgacg
     2641 atttcgcgga atccgccgcg cgggtcggcc tcgaagcggg gcgcgggctt gggcgcgtct
     2701 tcggtcgggc ggccgttcat gtagccctcc gccagcacca caccgtcggc caggcgcttg
     2761 atcgacacat aggtggccca accggtcggc gacgctgcat cgcgcgtgtt gagcacttcc
     2821 atcagcagcc tggcgttttg tgtcagcgca tcggtttcca cctgctgcgt gtcgaggctg
     2881 ccgccttttt gtctggccgc ggtggtgcgc atgaccacgt tgcggtccac cagcctgacg
     2941 cccgacgacg cgatagtctg catgaaggcg ctgcgcatct gcaggtccac ccgctcgacg
     3001 gggccgctgc gttgcgcctg ctggctcctg acctcctgcg aggtgatggt agtgttgccg
     3061 accaccgcgc cgctgacctg cgccgcggca taaccgggca cccgcaccgc ctccgcgctg
     3121 gaaacgccgg cgcgcagcgt gtctatgctg acctgcgacg cgctgccctg ctgcagtacg
     3181 tcggtgaact cacggttcca caacaccgca atcgtcgggc ggcccgcgcg cgcgtaagcg
     3241 ctgctgaaag ccgcgcggtt gaactgcggc ccggtggtaa cgaccaccgg gggcggcgtg
     3301 agcgtgtcgg gcatgtcccg gcggtaagcg ctttcaggaa tgacgggggc cggggcctgc
     3361 gcaaacgcag cggcagccag cacggccaag ccaagggaca gggcgggacg cttcatggag
     3421 agcctcttga ggtggagcga cggtcagccg gctttttatt tgagcggcac ctggtaggtc
     3481 tggcccggcg caaactgcga ctggccgcgg ttgccgtcgt tgatgtccac cgccaccttg
     3541 tcggtggcct gcgagatggc cgactcgccg taggtggcct ggccacgcgg cgccaggaac
     3601 acggttttcc aggcgctggg gggctcgctc tggccgcggt tgccggtaaa acgcgtgcgc
     3661 atggtcagca ccacatcgac atcggagcag ttgtagaccg tggtctgcac ggcggccaag
     3721 ccggtgccgg tgcgcgaggt ggaaatctcc tgcacgccga tgaccttggc gagttcgggc
     3781 gaaagcacgg ccaccgacac caggtgcggc gcaccggcct gctgctccca ggtccggcag
     3841 ccggaaaagg tcggaggggt ggactgggtc gcggcgcagc cggccagcac ggccgcggtc
     3901 acacctgcaa gaagaagacg gatcatgcgc aaagctccct gtggttctgg tcagcggtaa
     3961 atcacgtcgt cggctgcggc acgggcaaat tcgtaaatgc cgctccagac gatttcgccg
     4021 gactccaggt ccgtcatttc aaaggcgatc tgcgtgtagc gctggatcat tccggtcttg
     4081 gtgttgcggc tgtcgagcga gccgattttt ccggagagcc ggtaatcggc gccgagctgc
     4141 gccttggtca ggccggtggt accgacatcg gtggtgccgc tgcgcttgag cgcgcgctcc
     4201 tgctcgatca tggccgtgtt ctggcggttg atgaactgca tgcgcgaacg cgcggcgcgg
     4261 ttcaggttga ccatcaggcg ctgggtgatc aggttcttgt tgatcgcctg cgagctttca
     4321 ttgacgaagt actggctgtc gatgatgacg cgcgggcgct tgccggtctc ggcaaaaatc
     4381 tgctcggcca gcatgtcgcg catcatctgg tcggtcatgg ccaggatgtc ctggctttcc
     4441 acgccaacgc cggacaccgg ccccttgcga cccgggtcca catccatcga cgacacgccg
     4501 gtggcgtttt gcagcggcgg gttggtctgg caggcggcga ggctgcacac cagcagcaac
     4561 agcgaggcgg ttttggcggt ggttctggtc atgtccggct tctttcctgg caacggcgcg
     4621 cggcgccggg tatggcaacc aaacgcaaac aattttgtac aaaaggaggc tcttgtttac
     4681 aaaattagta tcgctatcac cgaaagccgg caccatccca taaattgggg gcaggtagcc
     4741 ctcgccccgc gctgcagggc aataccggag aagacgctaa aaaggggcag cctgccgccg
     4801 ccgggccggc ttatttgttg ccgcgcaggc gctcgatcag cccgttgagc tcgtccatgg
     4861 aggcgaactg gatggcgact tcgccggtgt cctcgcgccg gccattgcgt ttgacctgct
     4921 tcttgacgcg gacttcgacg tcggccatca gcaggtcggc cagctcttct tccacgcggc
     4981 gggtgtcgcg cgacttttcc ttggtgtttt tctgcggctt gagcgaaaac tcggcgctga
     5041 gcttcttgac caggctttcg gcctcgcgca ccgacatttt tt
//
LOCUS       DAIDFG010000231         5014 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_231_length_5014_cov_14.677556, whole genome shotgun sequence.
ACCESSION   DAIDFG010000231 DAIDFG010000000
VERSION     DAIDFG010000231.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_231_length_5014_cov_14.677556"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          941 a         1624 c         1615 g          834 t
ORIGIN      
        1 ggcgcgcccg agtcgccgcc gcacgcggtg aaagacggtg ccaagacgcc cggcaaggtg
       61 gtgatttatt cgacctgcta catcaactac aacgagcccg gcatcggcca cgacctgctc
      121 aaggtgctgg accacaacga agtgccttat gtgctggtcg aaaaagaaaa atgctgcggc
      181 atgcccaagc tggagctcgg cgacctcgat tcggtcaatg agagcaagga agccaacatc
      241 ccggtgttgg ccaaatacgc gcgggacggc tttgccatcc tgagcgccgt gccgagctgc
      301 acgctgatgt tcaaacagga aatcccgctg atgtaccccg gcgacgccga ggtgcacctg
      361 gtcaaggaac acatgtggga cccgttcgag tacctgatgg cacgcaagcg cgacggcctg
      421 ctcaagaccg ggtttccggt gccgctgggc aagatcagct accagattcc ctgccacggc
      481 cgggtgcaga acatcggcaa gaaaaccgag gaaatgctca agatggtgcc tggcaccagc
      541 gtgcacacgc acgaacgctg ttcgggccac gccggcacct tcggcgtgaa ggtgccctac
      601 caccagcagg ccatgaaaat cggcaaaccg ctgttcaagg ccatggccag ccacaaggac
      661 ggcggcctgc ccgacgtgat cagcagcgac tgcccgctgg gcggtcatca cattgcgcag
      721 ggctttgacg tcaaccagct cggcgcaccg ccacaaaaac acccgctgac cctggtccgc
      781 accgcttacg ggctgaagta gccagcaagg agcccatatg caagtcaccg gaaaaatcac
      841 cgcgggcaac ctcatgaccc tggaggccta cagcaagtac cgcaaggaaa acaaggccgc
      901 catcatggcg caccgccgct tgcgcagcgt gcgcctgggg gaacacatca acgtgcagtt
      961 cgaaagcgaa accagcatcc gttaccagat ccaggaaatg ttgcgtatcg aaaagatctt
     1021 cgaggaagaa ggcatcaacc aggagatcga ggcttatgcg cccttgatgc ctgacggcag
     1081 caactggaag gccaccgtga tgctggagta ccccgacgtc aacgagcgca agcgcgagct
     1141 cgcccggctg atcggcgtgg aagaccgcct gtttgtcgaa gtcgaaggcc acccgcgggt
     1201 atatgcgatt gccgatgagg acatggccag ggaaaacgac gagaaaacct cggccgtgca
     1261 ttttgtgcgt tttgagctca gcccggccat gtgcgccgcg gtcaaggcgg gtgccggcgt
     1321 caagctcggg tgcgaccaca cccactaccc ggcgcatgtg gcgattcctg ccgaatcgct
     1381 ggcgtcgctg gccagcgacc tgcgctgagc cgggcttttg gggcgttgcg ccgcccggct
     1441 gcggctggct gtcgtaaagt cgggactggc tttacttcac ccccagggac catgctgttt
     1501 ttactttcgc cggcgaagtc gctggatttc gccacgccgc cgcacgtcgc cgcgcatacc
     1561 gcaccgctgt tcatcaagca gtccaccgag ctgatcaagg tgctcaggga gaaaaccccg
     1621 gcccaggtca gcgagctgat gtcactgtcg gacacgctgg ccggcctcaa tgtggcgcgt
     1681 taccaggcct ggtcgccgaa gttcagcgtc aaaaatgcca agcaggccgt gctggccttc
     1741 aatggcgatg tctacgaggg gctggacgcc aaaagcctgc cggccgcgga cctcgactgg
     1801 gcgcaggcac acctgtgcat tctgagcggc ctgtacggcg tgctgcgtcc gctggacctg
     1861 atgcaaccct accgcctgga gatgggcacg cggctggcca cggcgcaggg cagcaacctt
     1921 taccagttct ggggcgagca gattgccgac tacctcaatg cgcgtgcggc cgccgacact
     1981 tcgccggtca tcgtcaacct ggccagtgag gagtatttca aggcggtgga ccgccgggcc
     2041 ctcaagccgc gcgtggtcag ctgcgtcttc gaggaattca agggcggcaa gtacaagatc
     2101 atcagctttc atgccaaacg cgcgcgcggc ctgatggtgc gttacgcgat cgacaggaag
     2161 ctggccaccg tcaggaagct cgagggcttc gacgccgaag gttaccgttt tgcaccggcg
     2221 gtgtccgggc ccgaccggct ggtgtttcgc cgccagcagg ccggctgagt cctgtgtcca
     2281 ccatgcgcga ctacgaggtc cagccccagc atgtgacgcc cgaggtgcag gcctgggtgc
     2341 gtgcccaggc cgccctgggc cacacggcgc ccaccatcct gaaggccctg cgcaagtcgg
     2401 gctggctggc ggacgtggca cgcgaggtga tggccgagac cctgcaagcg ccttccggcg
     2461 tgccggtgcc gcggcctgcc atcggtgcgg cgctgcggct ggacgccggc gaccggcagg
     2521 tcgatgtgct gatgcagatg agccagccgc acatcgtgct gttcggcaac ctgctcagtg
     2581 acgacgaatg tgacgcgctg atgcaggccg ccaggccgcg cctgtcgcgt tcgctgaccg
     2641 tggcggtcaa gaccggtggc gaggaactca atgacgaccg caccagccgc ggcatgtttt
     2701 tcacgcgcgg cgaaaacgag ctggtcagcc gcatcgaggc gcgtatcgcc cggctgctgg
     2761 actggccgct ggaaaacggc gaaggcctgc aggtgctgca gtacctgccg ggcgccgagt
     2821 acaagccgca ccacgattat ttcgatcccg ccgagcccgg cacggcgtcc atcctggagc
     2881 gcggcggcca gcgggtgggc accctgattc tttacctggg cgagcccgaa gccggcggcg
     2941 ccacggtgtt tcccgacatc gggctggaag tggtgccgca gcgcggtcat gcggtttttt
     3001 tcagctacga ccagcccgat gcctccacgc tgaccttgca tggcggcgcg ccggtgctgg
     3061 tcggcgaaaa atgggtggcc accaaatggc tgcgcgaaca tgaatttgtt taacacccct
     3121 gttgaggctc acttcgtgta gcctactctc cccttgcagg ggacggcccc tgcggcctgg
     3181 caaagccagt tccgcggccc ctgctggggg catccgtcta ccgcgttccg gcagaggtgg
     3241 acataatcac cagatgaaaa taaaagcaaa cggcataaac atcgaagtcg aggattcggg
     3301 tcccggcagc ggcgcgaagc cgcgtccggc ggtgctgctg atcatgggcc tgggcatgca
     3361 actggtggcc tggccaccgg ccatgatgca ggcgctggtc gacgccggtt accgcgtgat
     3421 ccgcctggac aaccgcgaca tcggcctgtc gcagaagttc gaccacctgg gccagcccaa
     3481 cctggtctgg gaaagcctca agtacaagct gggccttccc gtgaagtcgc cctattcgct
     3541 gcacgacatg gcggccgatg cgctggccgt gcttgacgcg ctgcagatca acaaggcgca
     3601 tgtggtcggt gtcagcatgg gcggcatgat tgcgcagcgc gtggcgctgg ccgcgccggc
     3661 gcgtgtgctg agcctgacca gcatcatgag ctccagcggc gcgcgcggcc tgccgcaggc
     3721 cgaaccgcat gtcctgcggg tgctgctgag ccggccggcc ggcaaggacc tgtcctccat
     3781 cgtggatcat tacgtcaagc tgttcaaggc catcggcagc ccggggtttc cggtggacga
     3841 cgcggaactg cgctcgcgca tcctggcggc ctccgaacgg agctttcatc cggccggcgt
     3901 gacgcgccag atgatggcca tcgtcgccga cggccgccgt gccgcggaac tggcggccct
     3961 gcgtttgccg gccctggtgg tgcacggcaa ggccgacccg ctggtgccgt ttgcctgcgg
     4021 cgaggacacg gcgcggcgca ttcaaggcgc ccggctgata ggcattgaag gcatgggcca
     4081 cgacctgccc gacggtgtgg tccagcgtct gctggagcca ctgattcccc accttgatgc
     4141 cgtacaggcc acaccatgaa tacccccgac cagtcccaac tgggcaagcc gacgccctac
     4201 atcgaccagt acgacgcttc gctgctgttt cccatccccc gcgccgtcaa gcgcgaggag
     4261 ctgggcctgg gcgagaacct gccttttttc ggggccgaca tgtggaccgt gttcgagctg
     4321 agctggctga acctgcgcgg caagccgcag gtggcgctgg cccacatcac cgtgccctgc
     4381 gagtcgccga acattgtcga gagcaagtcc ttcaagctct acctcaacag cttcaacaac
     4441 acgcgttttg cggatgcacg cgaagtgcgc gagcgcatcc gcgccgacct caacgccgcg
     4501 gtcggtgccg gcgtgggcgt caagacgctg gggcctgagc tgttcgacca ggagcccgtg
     4561 catgaactcg acggcctgaa cctggaccgg ctcgacgtgg agtgccagca ttacacaccg
     4621 gcgcccgagc tgctgtttgc cgaccatgaa gagccaccgg tcaccgaaac cctggtgtcc
     4681 aacctgctga aaagcaactg cctggtcacc ggccagccgg actggggcag cgtgcagatc
     4741 atttattcgg gcgcgccgat cgaccaggaa ggcctgctgc agtacatcgt gagttttcgc
     4801 aaccacaacg agtttcacga acagtgtgtc gagcgcatct tcatggacat ctggacgcgc
     4861 tgccggccgc tcaagctgtc ggtgtacgcg cgttacacac ggcgcggcgg gctggacatc
     4921 aacccgttcc gcacgagtta tccgcaggcc ttgccgccga atatccgcac ggcgcggcag
     4981 taggcttggg ggctgtcatt gcggacctga tccg
//
LOCUS       DAIDFG010000232         5008 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_232_length_5008_cov_17.929134, whole genome shotgun sequence.
ACCESSION   DAIDFG010000232 DAIDFG010000000
VERSION     DAIDFG010000232.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_232_length_5008_cov_17.929134"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          847 a         1661 c         1587 g          913 t
ORIGIN      
        1 aactccctgc ccccgagggg gccgctgcgc ctgcggtccg gcaaagccgg tcccgcggcc
       61 cctgcttggt tttagcttcc cggggtgccg gcgtgttggt ttgtcattgc ggacttgatc
      121 cgcaatccat gcgtgcacac tggcggcggt ggttcttgct gcatggaccc cgggtcaagc
      181 ccgggatgac gccgggaggg gtagccggtg tgatgcacgc cgtaaacttg tcattgcgaa
      241 ctcgatccgt aatccatgcc cgccaaccac acagcaggag tcagcaaaaa tgcccatcac
      301 caaaggattc cgcgccctcg tcgatgaggc catggcgcag gtcaccacgt attccgtcgc
      361 cgaggcccag gccaaacttg ccgacccgca ggtgcagatc gtcgacatcc gcgacgtgcg
      421 cgagctcgaa cgcgaaggca cggtgccggg tgccctgctg gcgccgcgcg gcatgctcga
      481 gttctgggtg gaccctgcct cgccctattt caagccggtg ttcggcgacg agggcaagca
      541 gttcatcctg ttctgcggtg ccggctggcg cagcgcgctg gccaccaaga cgctgcagga
      601 catgggcatg accaacgtgg cccacatcga cggcggcttt gccgagtgga aaaagcaggg
      661 cgccccgatg gaaaccctgg aagcgcacaa gcaggcccac gcggcgcgag ccaaaggctg
      721 acgttggctg cctgcccctg cagccgggcc gatgcgcgcg cccggccgct ggcttacgcc
      781 gcctgctgcg gccgctacct ggcgcacgac acgccggcgc ccgacgccga ggcgctgatg
      841 cgctcgcgct acaccgcctt tgtgctcgaa cgggccgact acctgctcgc cacctggcat
      901 gccagccacc gcccgccagg catcgagttc gaccccggcg tgaaatggct cgggctggac
      961 gtgcgccggc accggctgct ggacgacagc cacgccgaag tcgaatttgt cgcccgccag
     1021 aaaagcccgg gcagccccgc cgtgcgcctg cacgagcgca gccgctttgt gcgcgaagcc
     1081 ggccgttggt actatgtgga cggcgaccag ctgtagcgag acgtccggag cggcctggca
     1141 ggcctcgcac ccgctcagac cgctcagtcg cttgtaggaa cccgttcgcc ctgagcctgt
     1201 cgaagggtgt tggtccttgg cgatgttcaa tacaggccaa agccggcatt gacggggatt
     1261 atgagtaaaa aaggcctcca gcccttatcc cacctgcgcc agaagctcct gaatcaatag
     1321 cattttgcag agaggccacc cgtgttgaaa ttcgaagccg tgttgtttga ctgtgacggc
     1381 gtgctggtcg acagcgagcc catcaccaac ggcgtgctgc gcgacatgct ggaagaagcc
     1441 ggctgggcgc tcagcagcgc cgaatgcatg cgcctgttca tcggcaaagc tgtcaaggac
     1501 caggctgcgc tgatcgaagc caatacgggt cgccccttga cccaggagtg gctgcacgag
     1561 tttcgtggcc ggcgcaatga agcgcttgtc gaaggtctgc tgccgattcg cggcgccgtc
     1621 gaagccgtga cgcagatcca caccctgtac gagggccgca ttgcctgcgc ctccggcgcc
     1681 gaccgcttca aggtcgatct gcagctcgaa aaatgcggcc tcatgccctt cttcaagggc
     1741 cacatcttca gcgggcacga gctgccgcgc tccaaacctg cgcccgatgt ttacctggct
     1801 gctgccgcgt cgctgggcgt tgaccccaag cgttgcgcgg tggtggaaga caccgtgacg
     1861 ggtgtcgccg ccggcgtggc cgcaggcgcc accgtgttcg gctacagccc gcccgaagcc
     1921 gggcacgacg cgcccgacgc attgctcaag gccggcgcgg tgcacatttt tgtggacatg
     1981 actgccttgc cagcattgct gggctgagcc gccacaactc aggccagcgt catgtgtttg
     2041 gccaggtggc cgaacaccgt ggacacccgt ctcaggtgcc ttgactcggt gtgtgtcaac
     2101 agccaaagct ccgtctggca ttcatccagt gcatccgtca gggcgcgcag atcggttcga
     2161 tctctggcaa gaaaaattgg cagcaggcca atgcccatgc cttgccccac gatttccgcc
     2221 acggtgagaa tgctgtccac gcggtagcgc ggcgccactt tgggaaagtg ttttttgcgc
     2281 caggtcaccg aggggtggtc cggcagggcg tcgtccgggg cgacccagtt cactttgccg
     2341 gcgatgctcg cgtcgaagtg tttggggccg ccttttctgg cggcaaacaa ggccatgcgg
     2401 atcggcccga tatgtttgcc taccagatgc tggggcgggc gccgggtggc ccgcacggca
     2461 atgtccgcgt cgcgccgggt caggctggcc aactcattgc ctgtgtgcaa ctcgtagctc
     2521 agcaaagggt ggcttgacgg caacaaccgg agcgccggcg ccaccagccc atgcagaagg
     2581 gtgtcggtgg tggtgatgcg cacgctgccc gaaacctgtt ctgcctcggc ctgcgcggcc
     2641 gaacgggccg actcgagctg cacctccagc tgttccccat ggctggccag cacctgcgcc
     2701 agctccagcg gctggtagcc cgtgcgcgag cgctcaaaca ggcgttgacc caaacccttt
     2761 tcaatgcgct gcagggagcg gaacaccgtc gatgcatcca ccgcgagccg ctcgcccgct
     2821 tctgccaggg tgccggcccg caccagcgcc agcaccactt ccacgtcctg ggcgctgagc
     2881 ttgtattgcg ttgctgcatt catggtttgc gtgcgcgcct gttttcattg cgttattgca
     2941 atactacatt acacagacca atctacccaa tggagaaatg caatgcccga acctgacatc
     3001 aacaccaccg ccccttacgg catcgccttg cttcgcgtca gcctgggcgc catgtggatt
     3061 gcccacgcat tgttgaagct gtttgttttc acgctgcccg gaacagcgca atttttcgaa
     3121 agcgtgggtt atcccggtgc gctggcctac cccgtgtttg cggccgaact gctcggtggc
     3181 ctggccatcc tggtcgggct gtatgcgcga caggtttcgc tggcgctggt gcccgtcatg
     3241 gtggctgcag cgctggtgca tcttggcaat ggctgggtct tcaccagtca gggcggtggc
     3301 tgggagtacc cggtgttcct ggccgtggct tcggtgacct tgtggctggc cggcgacggc
     3361 gccctggcgc tgcgccgcag ccagcgcttt gtgcctgcct tgtgaccagg gagcagacag
     3421 tgacaatgac gccctcactc accctgatca gccacagcct gtgcccttac gtgcagcgcg
     3481 cggcgatcgt gctggccgaa aaaggcatcg tttttgagcg caaggacatt gacctggccc
     3541 acaaacccgc ctggtttctc aaagtctcgc cgttgggcaa aacgcccgtg ctgctggtgg
     3601 gcgaccaggc ggtgttcgag tcggcagtca tctgcgaata cctcgacgac accgccgctc
     3661 cgcgcctgca cccggaggcc gcgttgcagc gcgcgcagca ccgcagctgg atggagttcg
     3721 gctcggccgt tctcagcaac atcggcgctt tctacaacgc ccccagtgaa gaagcgctcg
     3781 tggccagtgc caccgcgctg cgcctgaagt ttcagcacat tgaagccgtg ctgggccagg
     3841 gcccgttctt cgccggcgaa cgcttcagca tggtggatgc agcctttggc ccggtcttca
     3901 ggtacttcga cgtgttcgac ggcatcggtg attttggtgt cttcagtgac acgccccgag
     3961 tgaacgcctg gcgatgcgcg ctggcgcagc gtgcctcggt gcgcaacgcc gtatcgcccg
     4021 actacccgaa actgttgcag gcctttctgc agcaacgcgg gtccgccttg tccaggcgca
     4081 tggcggcgcg gccggagctc agcgccgagg cgccacgttc ttgagggcct tgcccacgtc
     4141 gccctgccat gcagccgggc cctgcgcgag aggtatgcat aaaattggcc ttcagccctt
     4201 gtgtggcggg cgcaagcagc tatgaaaaca gtagctaaag cggcgggctg atccaatcgc
     4261 aggggcgttg gccggccgaa gcgcaagcca gcccttccgc agaaaaagcc ttcctgcggg
     4321 cgtttttttg acagcctggg gcaagatgtc gaaagtcggg ccagccgttc gtcgtgcagg
     4381 tgtgaccctc aaccaccgac ccaaggacat ctccatgcaa tacctctgcc ttgcctatta
     4441 cgaccccgaa aaaatggccg ccatggcgcc cgccgacatg caggcgctgg ccgcgcaatt
     4501 gccggccaaa gacgctgcac tcgcgcaaac cgggtgcctg gtctggagcg cctcgctcga
     4561 agggccccag gccaccttcg ccttgcgccc gcgcggcggc aagccggccg tgaccgacgg
     4621 gccttatgcc gaagccaagg agctggtggg cggcgtcatc ctcatcgaag cgcccaacaa
     4681 ggaggaagcc atacgcatcg cctcgctgca tcccgcggcc acgctgggtg aaagcatagg
     4741 ctgggggatc gaggtgcacc cgatcggggt gtgttcggtg aatatccaaa agtagcgggc
     4801 gcaggcttct gctcttgtgg cggcgccagt ctgccgcatt tcaccccggc cgctgcaggt
     4861 catcctggcc ccgtatgtca tcccggacct gatccgggat ccatgccccg cgtccccgcc
     4921 cgtagttcgg cgcgtcaaag gcaaaggcaa gggcagtggc aactcgtctt cgccctgctc
     4981 cctcgcaacg gcaagcctgc cgtgaccg
//
LOCUS       DAIDFG010000233         4974 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_233_length_4974_cov_18.602561, whole genome shotgun sequence.
ACCESSION   DAIDFG010000233 DAIDFG010000000
VERSION     DAIDFG010000233.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_233_length_4974_cov_18.602561"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          811 a         1734 c         1622 g          807 t
ORIGIN      
        1 cacgctggca gcgccaaggc gccgctggcg acaacaccca tcaaacacaa aaaagtgccg
       61 cgccggaccg ctggtgcagt gggcaacaag gacagctgat ggacccgaaa tctgaggcgc
      121 cgaccgtcgg ccccgagacc ctcccccgca aacgcctgag ccgacgcgcg atcctgattg
      181 gcagcctgat cgcggtgctg cttctcgccg gcaccggctg gctcgcctgg cgcctgaccc
      241 accccgcggc agacgccagc accgccgccc ctggcggcgc agcccgccgc ggaccgccag
      301 ccaccacggt tggtgttgcc accgccgagc gggccagcat tcccattctt ctggacgccc
      361 tgggcacggt cacaccgcaa gccacggtga aggtgcgtcc acaggtctcc ggcgtgttgc
      421 agaaagtgct gttcaaggaa gggcagatgc tgcgcgcggg cgaactgatg gccaccatcg
      481 acccgcgcca gtttgagctc gccctgcagc aagccagcgg ccagcgccag cgcgacgaag
      541 cccaactcga cagcgcgcgc gtgacgctgc agcgtttcaa gactctgctg gaccaggact
      601 cgattgcccg ccaggaggtc gactcccagg ccgcgctggt caagcagctc gagggcacgg
      661 tcgtgatcga caaggccaac gaaggcgcgg cgcgcctgaa cctgggctac acccgcgtgg
      721 tcgcaccggt cagcggccgc gtcggcctgc gcgcggtgga cgtgggcaat gtggtgaacc
      781 ccagcgatgc caatggcatt gccctgatca cccaggtcac cccgattgac gtggtgtttt
      841 ccattccgca ggaccaggtc ggcgaattgc agcaggccgt cagcgccggc accgtgatga
      901 aggtcaccgc gctggaccgc acccgctcgg gcacgctgga caccggcgtg tttgcctcgc
      961 tcgacaacca gatcgacacc accaccggca cggtcaaggc caaggcgcgt tttgccaaca
     1021 gcaagctcac gctgtttccc agccagttcg tcaatgtgca gctgctggtg cgtaccatcg
     1081 acaacgccgt ggtggtgccg gtcaccgccg tacgcctggg cgccagcggc gactatgtgt
     1141 atgtgctcaa tgcggcggag cgcaccgtga gcctgcgccc cgtcaaacgc gggccggcca
     1201 cggtggacaa aatcgtgatc gcctccggcc tgcaggtggg cgaacgcgtc atcaccgaag
     1261 gcgccgaccg gctcaaggac ggcgcccgtg tggtgctgcc tggcgatgcg cccggcgctg
     1321 gccgggctgg tgggcgcagg ccgcagggcg ccgcatcggc accgcgcgct gacaaccccg
     1381 cgcccggcgc cgcaccgata ccccggcccg ctgggtctgc ctctggtgca cctgcttctg
     1441 ctgccccggc gacggcaggc ccgccggttg cgctggcccc gccggcaccc ccagccgtca
     1501 gcgcaggcgc gccgcctggc gctgagcagc gcaagcgttt tctggaacag gtcaaagacg
     1561 accccgaagc gctggcgcgg cgcaccggcc tgctcgcagc gatagaccgg ggtgatgccg
     1621 ccgcgctggc gcgctggcac cagatcatgg aacggcggcg ccagggcgcc caggcgccgg
     1681 ctcaatgacg gcccccggac gactgcccgc atgagcccgt cgagtccctt cattcagcgg
     1741 ccggttgcca ccgcgctgct gatggtggcc atcgttctgg ccggcctggt cgggttcaaa
     1801 tttttgccgc tgtcggccct gccgcaggtg gactacccga ccatccaggt gcagacgctg
     1861 taccccggcg ccagccccga ggtgatggcg cagaccgtga ccgcgccgct ggagcgccag
     1921 ttcggccaga tggccgggct gcagcgcctg agttcgacca gcgcggccgg cgtctccatc
     1981 gtcacgctgc agttcggcct cggcctgggc ctggacgtgg ccgagcagca ggtgcaagcg
     2041 gccatcaatg ccggcggctc gctgctgccg gccgacctgc cggcgccgcc ggtgtacgcc
     2101 aaggtcaacc cggccgacgc gccggtgctc acgctcgcga tcacctccga cagcctgccg
     2161 ctcaccgagg tgcagaacat cgtcaacacc cggctggcgc tcaagatcag ccaggtcagc
     2221 ggcgtcggcc tggtctcgct gagcggcggc cagcggcccg ccgtgcgcat ccaggcggac
     2281 acgcgcgccc tggcttctta cggcctcggg ctcgattcgc tgcgcacggc gattgcggcg
     2341 gccaatgcca acggcgccaa gggcagcatc gacggcccga cgcgttcata ttccatcaat
     2401 tccaacgacc agctgctggc agcggcggag tacaagaacc tgatcattgc ctttcgcaac
     2461 ggcgcggcgg tgcgtgtggc cgatgtggcg caggtggtgg acagcgctga aaacgtcaag
     2521 ctcggcgcct ggtcgggtct gaagccggcg atcatcctga acgtgcagcg ccagcccggc
     2581 gccaacgtga ttgccaccgt ggacgcgatc aaggcacgcc tgcccgagtt gcaggccggc
     2641 ctgccgaccg ccatgaaggt cgatgtgctg agcgaccgca ccaccggcat acgcgcctcg
     2701 gtgctgcatg tggagatcga gctgctgctg gccgtggtca tggtggtgct ggtgattttt
     2761 gcgtttttgc gcaacctccg cgccaccgtg atcgccagcc tggccgtgcc gatttcgctg
     2821 ataggcacct gcggcgccat gtacctgctg ggctacagcc tgaacaacct gagcctgatg
     2881 gcgctgacga ttgccaccgg ttttgtggtg gacgacgcga tcgtgatgat agaaaacatc
     2941 tcgcgctaca tcgaaaaagg cgaggccccg atggccgccg cgctcaaggg cgcgcagcag
     3001 atcggcttca ccatcatctc gctgacggtc tcgctgatcg ccgtgctgat tccgctgctc
     3061 ttcatgggtg acgtggtcgg ccggctgttt cgcgagttcg cggtcacgct ggccatcacc
     3121 atcctgatct cggcggtggt ctcgttgacc cttgtgccca tgatgtcagc gcgctggctc
     3181 agacatgaac cggacaccgg cggcagcccg accggcgcga aactgcagaa gtttttcgat
     3241 ggcgtgatca cgcgttacga cggctggctc agctgggtgc tgcggcacca gggcgccacg
     3301 ctgctggtgg cactggccac gctgctgctc accgtgctgc tctatgtgct gattcccaag
     3361 ggtctgttcc cgacgcagga cactggccag ctgcaggccc gcatcgagac ctcccagtcg
     3421 gtgtcgtatg cgcgcatggc cgagctgcag caggcggcgg ccaaagccat tctggaagac
     3481 cccgacgtgg acacgctgag ttccttcatc ggcgtggacg ccgccaacaa caccatgctg
     3541 cacaccggcc gcatgctgat caacctgaaa agcagccgca gcaccagcca gcaggagacc
     3601 atggaccggc tgcagcgccg ggccggggcg gtggcgggcg tcaccatgta tctgcagccg
     3661 acgcaggacc tgaccatcga cgccgagacc ggcccgacgc agttccgcgt ttcgctcgaa
     3721 ggttcggaca cggccacggt gacgctgtgg gccaacaagc tggcgcagcg catgcaggag
     3781 ctgccggcgc tgcgcaacat cgcctcggac gccggtgcca ccggcacctc ggcctacatc
     3841 tcggtggacc gcgacaccgc ctcacgcgtg ggcgtgacaa cctcggcgat tgacgacgcg
     3901 ctgtacagcg cttttggcca gcgcatcgtc tcgacgattt tcaccgagac caaccagtac
     3961 cgcgtgatcc tcgaggccct gcccgacccc ctgatgaccg cggcctcgct gggcagcctg
     4021 ccgctcaaaa ccggctcggg cgtcaccacc ccgctggcca gcatcgccac catcaccgag
     4081 cagcccgcac cgctgcagat cacccgcgtg gcccagtacc cggcgacgac gctgggtttc
     4141 gacacagcac cgggcgtctc gctcggcaag gcggtgggcg aggtcaagca ggcagcgaag
     4201 gacatcgccc tgcccgccag cgtgaccctg accttcctcg gcgcggcggg ggcctaccag
     4261 acctcgctgt ccagccagct gtggctgatc ctggcggcgg tgatctgcgt ctacatcgtg
     4321 ctcggcgtgt tgtatgaaag ttatatccac ccgctgacga ttctctcgac cctgccttcg
     4381 gccggcgtgg gtgcgctgct gatgctgatg atcagcggct ccgaccttgg ggtgatcggc
     4441 atcatcggca tcattttgct gatcggcatc gtgaaaaaga acgcgatcat gatgatcgac
     4501 ttcgccatcg acgccgaacg cagcgaaggc aagtcgccgc aggaagcgat tcatcaggcg
     4561 gcgctgctgc gcttccggcc catcctcatg accacgctgg ccgccctgtt tgccgccgtg
     4621 ccgctgatgc tcggatgggg cgaaggggcc gaactgcgcc ggccgctggg gctggccatc
     4681 ttcggcgggc tcatcgtcag ccaggtgctc acgctgttca ccacgccggt gatttatctg
     4741 gcgtttgacc ggatggggca gaggttcagg cggagggctt cggtgtgaac accctcctcc
     4801 ttccgcgggt tgtcatcccg gacttgatcc gggatccatg ttcgaagacc accaccgttc
     4861 gggctgagct tggtctgtcc agagcttgtc gaagggtcga agcctgtgcc agcttgaaag
     4921 ttggcggtgc ttcagacaag ccgggtctcg ccccggcggg cgactcactt tctc
//
LOCUS       DAIDFG010000234         4964 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_234_length_4964_cov_18.199837, whole genome shotgun sequence.
ACCESSION   DAIDFG010000234 DAIDFG010000000
VERSION     DAIDFG010000234.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_234_length_4964_cov_18.199837"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          959 a         1578 c         1590 g          837 t
ORIGIN      
        1 catgccgggc cgtgtcaccg tttaccggag gacagttgca tgaagttcat tttcgaagtc
       61 cgcatgaaac ccggctacac ggtggacgag tatgccgccg cgtggatcag tgccagtgaa
      121 atcatccagc gcacgccggg cgcgcgtggc acttacctgc accgcaagat cggttcgccc
      181 gacaccctgc tggcgattgc cacctgggac accaaggcgc accgcgatgc caaggacgac
      241 cgccgcgacg ccaccgtacg cgccatcctc gacaaacatg cccagcactg caacgtcacg
      301 gtgatcggcg agtttgaaga gcccgagtgg tcggtgctgc cggaacccgg tgcgacgtga
      361 gcgtcttgta gggcttgcac gtgacacatg gaccagcagc tgctcgactt caggaacacc
      421 tttcgcccat cctctcaaaa aaatcggagc ttagtggttt ctcccagtct gtacgcattg
      481 cagatgctcc gctattcttg cttttttttc aggagggatg catggaatcg tctttgaacg
      541 tcgaagacca cggcgagtgg ctcagcttcg gaatgtcgca tgccggcagc accattccgg
      601 tcaaaatttc ccgggcagcg atggaggagc atttcgacgc gacccagggg ccggactcgc
      661 tgaaaaaggc ctatgcactc gacgccgaga tgattcatgc ccgtgcggcc gaccacatcg
      721 tggcgggcgc cagctacacg ccggaccatc cgctggtgct gggacccgaa cacttctagg
      781 agcctgtcgg gctcaggaat cgtcggctgc aaatcgaccg cagcgaccca ttttcactgt
      841 ctttttgtcc catagctggg ctaggggcct gcaccggcgg ataccgcatg tgccggggag
      901 gctgtgaaca ctccgttctt tgctccgctt tttatagctg ctcgcgcccg cgtgacaagg
      961 gccggcggcc gattttgctc gtaaaactca gcccacccga cgaaacgtca ggttgatgcg
     1021 gcgccggccc agcagcgtgt gttcgccatc ggccagcggt gccacgccgt ggaaggccag
     1081 gcgcgacggt ccgccccaga ccaccacgtc gccatgctgc agacgatggc gctgcggcct
     1141 gtccttgcgg ctgggcgtac cgaacagaaa caccgcgggc aggcccaggg acaccgagac
     1201 gatgggggcg aaaaagtcct gctcgtcccg gtcctggtgt agcgagagcc gtgcacccgg
     1261 ctcgtaacgg ttgacaaggc aggcgtcggg ctcgaagtgg gcaaagccgg cctgcgctgc
     1321 cgcgcgcacg gccagcttgc gcaggatcgc gggcatggcg ggccagggct ggcccgacag
     1381 cgggtcccgc gcatcgtagc ggtagccgct gcggtccgac acccagccca gcgcgccgca
     1441 attgctcatg gccaccgaca tcgtgcgccc gcccggcgtg accagatggc gaaacagcga
     1501 ctggctggtg acctcagcga cggcctgcag cagggctgcg gcgtcctcgc gtgcaaaact
     1561 gcgcagcaac atggcaccgg gtgccagcgt ttcacgcgac accgccacgg gcaactcgtc
     1621 aaaaagatca gcggtcaagc tttctcctgc gcaaattcgg gcgctactgt gcatcatgaa
     1681 aaatcacacc cagcgtgtgg cgatggcccg agcgtaccgt gctgacgccg tggcgcatgt
     1741 tgaccctgta aaaacccctg ctgccctgca ccggccggtg gtgcacggca aacaccacgg
     1801 cgtcgccctg cgccagggga atcacctcag ggcgcgactg catgcgcggg cgctgctcgg
     1861 tcagcacaaa ctcgccgccg gtgaagtcgt cccccggccg cgacagcagc accgccacct
     1921 gcagtggaaa cacatgctct ccatacaggt cctggtgcag gcagttgtag tcgccttcgg
     1981 cgtactggag cagcagcggc gtgggccgcg tctggccagc cgcgtggcaa cgcgccagaa
     2041 aatccgcatg gcctggcggg taacgcacgg cgatgcccat ggcttcgttc cagcggttgg
     2101 ccacgtccac cagccgggga tacagcgtgc tgcgcagttg ctgaaccaag tcgggcagcg
     2161 ggtaattgaa gtactggtat tcgccctggc caaagccgtg gcgcgccatc acgacgcggc
     2221 tgcgaaacag cgctttttgc cggtaaagcg cggcggttgc cgcacactgg gcagggctca
     2281 gcagctgcgg caacacggcg ctgccgcgtc tttgcagttc tacggccagt gccggccagc
     2341 cggtttcagc cggcctgtgc atcaacaagg tgttcatggc gggtgggtgt cctgggaaaa
     2401 actcaagcgt aggccgggag ccgtggccag gctcgccgga aacggacatc ccgccgattt
     2461 atcgcagggt gccgaggcgc cctggttcat gcatgaaatt ggcctccagc ccaatgtaga
     2521 cgggcgccaa cagctacttt attgatagcg tggcggggaa ccgtgcatgc cggtgctgcc
     2581 tgcgccgtcc agccccagca gggcaagaaa gcgctggccg agcgtgccgg cgccggatcg
     2641 cgcggcagcc accggcacag acgggtggcc tgcgggctgg ctaccggcaa actccggcag
     2701 gcaaacagcc agctcctcaa aaccggccac gatgcgcggc ccgacccaca gccgcaccca
     2761 gcgcgccatg gccacacgct ggtcacccat gcgcaggctg gccgcaaaat gcggtgtggc
     2821 aaaggacctg gcgacctgcc gctcgacaaa aggcgtggct tcgtcggcca cccagtccag
     2881 gaacaccggc gacaacttct gcgggctccc gcagacgagc tcgaacacca gctcgtcgag
     2941 cgcggaaaca gcggtgaggc aagcctcttt gaggccaagg gtgtgggtgc ggatcatgag
     3001 ggttctccag aaaaatcaac cagcggcagg cctgtcaacc cgtgcccccg cctgcatcca
     3061 taccggcggc aatgcgccgg gacaacaagc gtgatttttt gaaggacaag cagcagagcc
     3121 atgcatgctt aacggcaggc tgcctgacaa gttgagggcc agacgcggaa ccgggagaca
     3181 cggtccgcgc cggcagcatg ggcttcagcc gatgtgcgac cgcgtccagc gcacgatgtc
     3241 ggcggcgccc atggcgccgg gctggcgcgc gacctcgcgg ccgttcacga acagcgccag
     3301 cgtgggaatg ctgcggatgt tgaagcgtgc gcccaggttc ggggaggcct cggtgtccac
     3361 cttgaccacg cgaaccatgg gttcgagctg cgcggcggcc tgctcatagg ccggcgccat
     3421 ctgcagacag gggccacacc acggcgccca gaaatcgacg agtaccggga tctggttgcg
     3481 cgtgatgtgg cggtcaaacg ccgcttcgtc gaggttgacc gggtgccggg tgaacagcgc
     3541 ctgatggcag ctgccgcagt cgggcgcctt gtccaggtcc gcgcttttga cgcggttggt
     3601 cgtgtggcag tgcggacaaa cgatatgcag ggaatccgtc atctgtacct cgtcaacatc
     3661 ctgaatcagt tgttgttggc ttcgtccagc gccttgcacg acggcgcgca cacggcgtgg
     3721 gacttgccca gcaccttgcg tgtgcccatc tgcgagcggg gacggtgttt tccgcacatg
     3781 aagcacgaca tcgtggcagc accgaaagaa gcggacgcga caaacgggga gcccgatacc
     3841 ttggatttgt aacgcagtcc gtcggcaagg acggtggttt tggcatcagc tctggccatg
     3901 ggtgtttcct tggttgggtt ggcgcattgc gcgctctatg gcttgcctgg cctgccgttc
     3961 ggcagccagc gccggatcca gcggcgagcg gcacaggccc gcatgctggt aatagacatt
     4021 ttcgtacaat tcggccgcgg ccgggtgagt gtccagcaaa gcacccaatt cgcgaccggg
     4081 cgctacgccg cccaaggcct gaaccaggtg cgtcaccacc gaacggtatt gctccggacc
     4141 gaccggcacg gtactgtgct ccagccgctc gagcagctca gccagcacgt gggtcacggt
     4201 caggtcggga ctgaaagaag gggtttgggt cgtgttcatg cctctgaaac tgggggcttt
     4261 tggccgaaaa acaagccaac atgccctggc cccgaagatc actatgcttt tgatagctgg
     4321 cagcgcttgt ctgacaaggg gtgaaggcct attttgcatc aaatgcaggg cgccaccagc
     4381 ttcgcgtccg gtgacctggt gggagcaccg tttccgggtg cgaatctggt tggtggtgca
     4441 cgggtcctgc ttgcgcgacc ctgccagcgc aacggctaat cccgggcagc tggaaacatt
     4501 cataaaatga cggcgtgggg tcatggtctg cgttccggtc tgaagtctga agagccgcag
     4561 gccaaacaag gacgaattag atttcagttc ttcataccca aaacatctat aacgacatct
     4621 cacgaaagca atcacatggc acgcaccgtc aaatgcatca aactgggccg ggaagccgaa
     4681 ggtctggact tcccccccta ccccggcccg ctgggcaagc gcctgtggga agaagtcagc
     4741 aaggaagcct gggcagagtg gatgaagcag cagaccatgc tggtcaatga aaaccgcctg
     4801 aacctcgccg acgcccgcgc ccgccagtac ctggcgcgcc agatggaaaa ccactttttt
     4861 ggcaccggcg ccgacaaggt ccaaggctac gtgcccccga cggaataagg cccccacggt
     4921 tgctcactgc gtgtagcgcc ctgccccccg agggggccgc tgcg
//
LOCUS       DAIDFG010000235         4922 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_235_length_4922_cov_17.470105, whole genome shotgun sequence.
ACCESSION   DAIDFG010000235 DAIDFG010000000
VERSION     DAIDFG010000235.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_235_length_4922_cov_17.470105"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          847 a         1594 c         1577 g          904 t
ORIGIN      
        1 gccgctgatc tcgggcagca gcgcatcgga aattactatg gttttgatag ctgcctgcgc
       61 ccattcagcg ggggccagag gccagaatga ttctgaaaat acggtcatcg ccggggtcag
      121 cccgcgcagc agcgcggcgc ggcacaggtg gtcgccctcc agccagaccc gtccctgctt
      181 gcggtaggcc gtgctgtcct gtgagagccg gcgtaaatcc ttgaccaggg cgttgtcgcg
      241 cgaggtgaca aacacaggcg ctgccggcgc gggcgagctc aacgcagaac ctcggccacg
      301 gggctgaaag accgccggtg ttgcgggcag gcgccgtgct cgcgcagcgc agccaggtgc
      361 tcggccgtgc cataaccttt gtggccggca aaaccgtatt gcgggtattc cagatggaat
      421 tgctcgcacc agcggtcccg gtgcaccttg gccaggatgg acgcggcgga gatggccggc
      481 accagcgcgt cgcccttgac aatggcttcg gccagcatgc tgagcaccgg cagccggttg
      541 ccatcgacct gcaccttcac gggcttcaga cgcaagccct cgaccgcgcg tttcatcgcc
      601 agcatcgtgg cctgcaggat gttgagcctg tcgatttcct cgacactggc ctcggcaatc
      661 gagcagcaca gggctttggc gaggatttcc tcgtagagct gctcccggcg ccgcgccgtc
      721 agcaccttgg agtccgccag acccttgatg ggctggagat cgtccagaat caccgcggcc
      781 gccaccaccg ggccggccag cggcccgcgg ccggcctcgt ccacaccggc ccacagtccg
      841 ggcgtgtccc aggagagggt gacctgttca gccttcaaga atgttctgga tcgcatcggt
      901 ggccagttgg gcggtatcgc gctgcagctc gtggtgcagc tgtgtgaatc gttgttcgac
      961 agcagctatt ttttcgggtg cggagatttt cgcatctatc cattgcagca ccgcagcggc
     1021 cagcgcctgc ggcgtggcgg catcctgcag cagttcgggt acgacaaaat cgcggcacag
     1081 aatgttgggc aggcctaccc agggctgcag tttcttgcgg cgcatgatct gccaggacag
     1141 ccagttcatg ttgtaggcaa tcaccatggg acgcttgaac agcgccgcct ccagcgtcgc
     1201 agtgccgctg gcaatcagcg tgacatcaca ggccgccagc accgtgtgcg actgcccggc
     1261 gacgatctgc acctttgcca gcacacccgc gtcctgcgcc gcctgctcga tctgctggcg
     1321 caaggcgggc accgcgggaa ctactatttt gatagcaggc tgcgcctgct ggacaagggc
     1381 tgcagcccga aagaatctgt cagccagata tttaatctcg gagcggcggc tgcccggcaa
     1441 caccgccagc accagatcat ccgctgccag gcccagctgt tgccgggccg ccgccttgtc
     1501 gggctgcatc gggatcatgc cggccagcgg gtgacccacg taggtggcgg caatgccgtg
     1561 ttgcgccagc aaggccggct caaaggggaa gatgcacaac acgtggtcga cgctgcggcg
     1621 gattttttcg acacggtcgg cgcgccaggc ccagaccgag gggctgatga aatgaacggt
     1681 gcgtatgccg gctgccttga gatcggcctc gaggtcgagg ttgaagtcgg gtgcatcaac
     1741 gccgatgaac acatcgggcc gtaactgcag cagccgttcc cggagttgct tgcggatgcc
     1801 gacaatttcg cggtagtgcc gcaacacctc gacataaccg cgcaccgcca gtttctcgct
     1861 gggccaccag ggtgaaaagc cgcggcgcgc catctgcggc ccgccgatgc ccaccgccgt
     1921 caacccgggc cagcgtgcac aaaacccgtc gagcaacaaa ccggccagca ggtcccccga
     1981 ggtttcgcca gcgaccaggg ccatgcgggg agtatttgcc gcagaggacc cggccgcctc
     2041 tgtacggctt ggcacagggc ttggcgcatc ggacatcgtc gcttaccgga cgatgccgcg
     2101 ttgcggtgag ctgtccgcca gaaaggtcaa catcgcctgc acatcaggcg ccgcttcggg
     2161 atgcccggcg gccagttctg caatgcgcgc cttcgcctgc tccagcgtca ggtcctcacg
     2221 gtacagggcc ttgtgcatgg ccttgacggc cgcaatgcgg tcgggcgtgt agccgcgccg
     2281 gcgcaggccc tcatagttca tcgaacgcgc ctcggcgggc tggccctggc acatcacgaa
     2341 cggcggcaga tcggcaaaca acagcgaaca catggcggtc atgctgtgcg cgccaagccg
     2401 gacaaactgg tgcacgacgg tgaagccgcc gaggatcacc cagtcgccga cgtgcacatg
     2461 gccggccagc tgggagttgt tggcaaaaat cgtgtggttg ccgacatcgc agtcgtgcgc
     2521 cagatgcacg tacgccatga tccagttgtc gtcgccaacc cgcgtcaccc cggtgtcgcc
     2581 gggtgagccg atattgaagg tgcagaactc gcgaatggtg ttgcgctcgc caatgaccag
     2641 ctcgcagggc tcacccgcgt acttcttgtc ctgcggaatg gcccccagcg agttgaactg
     2701 aaaaatgcgg ttgtcgcgtc ctatcgtcgt gtggccctcg atgacgcaat gcgcgcctat
     2761 cgtcgtgccg gccgcgacct tgacgtgcgg gccgatcacc gtgtacggcc ccacgctgac
     2821 gctgctgtcg agctgggcct ggggatcaac cagtgctgtg ggatggatgc ctgtcacgga
     2881 aaaaccttct cttgccggga cagatcaggc gatagtgcgc atggtgcaca tcagctcggc
     2941 ctcgcagacc atgttgtcgt ccacccgggt cacacccttg aacttgaaca cgccggcctt
     3001 gagccgctcc agcgtgacct ccatgatcag ctggtcgccc ggctccaccg gccgcttgaa
     3061 tcgcgcgccg tcgatgccgg caaagtaata cacggtcttg tcatcggggg tcacaccctg
     3121 catgtcgaac gccagcaggg cagcagcctg cgccatggct tcgagcatca gcacacccgg
     3181 catcaccggc cggtgcggaa agtgcccggt aaaaaacggc tcgttgatcg tgacgttctt
     3241 cagcgccttg atgcgcttgc ctttttccag ttccagcaca cgatccacca gcaggaaggg
     3301 gaagcggtgc gggagctgct tgagaatttg gtgaatgtcc atcatgattt ttcttcggtt
     3361 tttccagagg ctttgtcagt cgtttgctca agcatcttga ggcgttcacg caaggcgtga
     3421 agctgcttga gtgtggccgc atttttttcc cactgcgcat tgtcgtcgat gggaaacagc
     3481 ccggtgtaat gccccggctt gtgaatcgaa cgggtgacca ccgaagcggc ggaaatatgc
     3541 acatggtcgg ccaggctcag atggcccagc accaccgcgc caccgcccac ggtgcaatgc
     3601 gcaccgatgg tggcgctgcc cgccaccccg gcacagccgg ccatggccgt gtgggcgccc
     3661 acacgcacgt tgtggccgat ctgcaccagg ttgtccagct tgaccccgtc ttcgatcacg
     3721 gtgtcctgca gcgcgccgcg gtctatgcag gtattggcgc ctatctcaac gtcgttgccg
     3781 atgcgcacgg cgcccagctg ttcgattttt tcccagcggc cggcatgcgg cgcgaaaccg
     3841 aaaccgtcgg cgccgatcac ggcccccggg tgcacaatgc agcgctcgcc gagggagcag
     3901 ccgtctgcca ccgtcacgcg ggccgacagg cgcgtttcgg caccgatgtg ggcatcgttg
     3961 ccgatcacgc tgtgcgcgcc cacctggaca ccggcgtcca gcaccgcacg ggcgccaatg
     4021 caggcaaagg cgcccaccac cacaccggct gccagacggg cctgggggtc gatgaaggcg
     4081 ctggggtgga gagcagtccc accggatgcg ctggacggtg catgctcgcg cttccagagc
     4141 tgggtgatgc gggcaaagta gaaatacggg tcatcgacga tgatgcaggc gccgcgctgc
     4201 atggccgccg ccgcggcgcc aggccctacc accacacagg cggcggcgga ccgggcgagc
     4261 ttgccctggt atttggggtg gctgagaaaa gtcagctcgg ccgcgctggc cgattcgagc
     4321 ggggcgagcc gctcgaccag gacatccggg tctcccacca gccgggcgtc aaattcgccc
     4381 gccagcgctg caacaatgtc cccgagtctg agcgtcacac ctggcgtgtc cctgaatgcg
     4441 ggcttatctg gcggcgttga gcgccctgat caccttgtcg gtgatgtcat gcttggggtt
     4501 ggcgtaaacc gcttcctgca ggatgaggtc gtacttctcg gcctcggcaa cggtcttgac
     4561 gaccttgttg gcgcgctcga tgacctgctg cagctcttcg tttttgcgcg cgttgaggtc
     4621 ttcctggaat tcgcggcgct tgcgctggaa atcccggtcc tgttccacga gctgcttctg
     4681 gcgggccacg cgctggctct ccgccagggt gggtgcctcg cggtcgaact tgtcggccat
     4741 ggccttgagc tgtgtcccga cgtcggtaag atctttctcg cgcttgccga attcctgctc
     4801 cagcttggcc tgggccgcct tggcgctgtt ggcttcgcga aagatgcggt caatgttgac
     4861 gatgccgatc ttgaaatcct gcgcgcctgc cgcaaacgcg gccagcgcca tcacggcagc
     4921 ga
//
LOCUS       DAIDFG010000236         4888 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_236_length_4888_cov_15.635009, whole genome shotgun sequence.
ACCESSION   DAIDFG010000236 DAIDFG010000000
VERSION     DAIDFG010000236.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_236_length_4888_cov_15.635009"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          941 a         1582 c         1383 g          982 t
ORIGIN      
        1 cgctccatgg cgcccaccgc atcctgcgtg tcggtctgaa tggctttcac cagcgcagaa
       61 atctggcgcg tcgcatctgc ggaacgctca gccagtcgtt gcacctcctc ggcaaccacc
      121 gagaatccgc gtccggcttc accagcggac gcggcctgaa tggccgcgtt gagcgccagc
      181 acgttggtct gttcggtaat gtccgaaatc agttcggtga tctcgccaat ctcctgggac
      241 gactcaccga gccgcttgat ccgcttggag gtttcctgga tctggtcgcg gatggagttc
      301 atgccgccga tggcgttttg caccgcctgc aaacccgttt cggcggcggt cagcgattgc
      361 cgtgccacct gggaagattc ctgggcctgg ctggacacct ggttgattcg ggtggccatg
      421 tcgagcaccg actggccggt ttcgcgaatt tcccgcagct gttccgtgga cgctgccagc
      481 aactcggtcg aggtgctttc cacctgggcc gtggtttgcg ccacccgggt caccgtgttc
      541 tgcacactgc ccaccagttg ccgcaactct tccaccgtgt agttcaccga gtccgcaatg
      601 gcgccggtga tgtcctcagt caccgtcgcc tcctgggtca ggtcaccttc agccactgtc
      661 tgcaactcgt tcatcaatcg caaaatggcg gcctgattgg cgtcattgac gcgcttggct
      721 tcctgctcct ggcctttggc ttcgagccgc tgattttcag caacaccctg gcgctgccgg
      781 ctatccagca cctgcaaacg agccagacct gcagcacaaa gaagcgcaaa ggcagccgca
      841 atagccagcg tcgtcaaggc cccggcaccc agaccagttt gcgacgacag cttgccctgg
      901 atatcttcca gaccccgacg caagggctcg ctgtcagcaa tgatcgacgc ctgggcctca
      961 cgagccgaca ccagaccctg caagttgccg agaattgcgc cggcctgcac gcgggtctgc
     1021 tcatacagcg ccatcagcgc ctcgagccgt tcgcgggttt gcgggtcctt ggaacccgtc
     1081 aggcggagct cgggactgcc ctcctgcagg ccctgcgcaa tttccttgaa cgagttcaaa
     1141 tccttgccca gcaggaacac ggcttcgggg ctcacgcctt ccatcgtcag aaattcgttg
     1201 gcggatttac cgatacgctg ggtcaacatc acgagctggc cggccgcaga aatttctgcg
     1261 ggtgccgcgt tctgctggag cttgagcgaa gacaccgtct cggcgatttc aagcagatcc
     1321 gaggactgcc ggttgatggt acgcagcgcg ttgcccactt gcgtcaaaat cgcctgctgg
     1381 gccatcacag ttgatgcatt tttctcggca cgctccatca gcggcgtcac ggtgtcgaca
     1441 tctggctgca gggtaacagc gaccggctcc agtcgcaggg actcatcgcc cgacctcagg
     1501 ccccgcacgg tttttgccag cacgtccgaa ctctcgcgca cgtccggaaa agcctgggca
     1561 ctgcccacca gcgcctgaga cacggacttc gcaagccgct gcgactgcat cagcgattgt
     1621 ccggtggcag ccacctgctg cgcgaccctg tccgcctggt tcagtgcgac aaaagtcacg
     1681 gaaccgagca ccacgaccgc aaaagcgagc aagatcacca ggatccgctg gtgctcaccc
     1741 atggaccttc ggcccaggac aggcaaggcc agcttttcaa cattttcgga aacgtcctcg
     1801 gcggactgac tttcaggacc gtcaccgtcg cccaggcgcg ccatggaggc cgaacccgaa
     1861 gcgtccatgg cccccgttgc aaggggatcg accgacatgt ccagagagac accatccatg
     1921 ctgtcctcgg ggggggcagg ctttgttttg aacagcttct gaatgacaga catggtgagg
     1981 ccttcctggg aaaaaaacgt aatagcgcta ggcgctgatg gtcaaaaaat gggcttgttg
     2041 cgccagcaac tgcaggttga tttcctgcca atgcacaccg ttcgcgtcca catagctgtg
     2101 gccgaaaaac tccggcgcgt cgggtgatct ttcggcgaag tccgtgaatg cttccgggtt
     2161 gcgcagaccg gccagcttgt cgatctgcaa ggcgcaattg atctccagag cgctgttgag
     2221 cgcaaccaga cgggactcgg tgcgtgccag ctcactgcgc gccggcggca ttttgccggt
     2281 cacaaaactc gccagatcaa ccacaccgta caggccgcca cgcagattgg ccacaccggc
     2341 aaaccaggct tgcgtgtagg gcacattctg cgcactgacc caggggaaaa tttctccgga
     2401 ctgattcagg gggaagaggt atttcccccc ccctgcttca acagccagcc aggacgccga
     2461 aacaccctgt gtcttggccg ccttcagcct gtcggcaaga cggctttgca gttctctaag
     2521 tgcttgtcga ttggccatgc tttagcggga tcggtgcgga atcagcccag cgccttgatt
     2581 ttggccatca ggtcgtcggc atccaccggc ttggtgatgt agtccttggc gccctggcgc
     2641 atgccccaga cgcggtcggt ttcctgattt ttgctggtac acatgatgat gggcacgtcc
     2701 gcgtacagag ggtcccgggt aatggcgcgc gtgagctgga aaccattttg cccgggcatc
     2761 accacgtcca tcaggatcag gtcaggcttg tcctcggcca gccgacgaaa ggcgtcttcc
     2821 gcgttctcag cggttttcac ggcaaaacca tttttgacca gcaggtcggt catgaacatc
     2881 aactctgttt tagagtcatc cacaatcaga atttttttaa tcgccattac gctactcctt
     2941 gttttgcgct gcccagttcc tgaacggtgt gcagcaactg gtctttggta aatggtttgg
     3001 tcaggtagtc ctgcgagccg accatcctgc ctcttgcctt gtcgaaaaca ccgtccttgg
     3061 aagacagcat cacaatcggc acattggaaa atttagggtt gcgcttgatg atggcgcagg
     3121 tctgataacc gtccagacgt ggcatcagga tgtcgcagaa gatcaggtcg ggcaggtagt
     3181 cgttgacttt ggagagcgcg tcaaaaccgt cctccgccag caacacctca tggccgccct
     3241 gcttcaggaa gatctccgca ctgcgccgga tcgtgttgct gtcgtcgatg accaagacct
     3301 tgagccccga actggtaata ctcactgact gtgctccttg taccgcgccg ccggcccggc
     3361 accgcccatc gcgcgttttt gtttagacac ctggcgcggc cattcagatt tgaaccattt
     3421 caaagtcttc cttgcgcgca ccgcattcag gacaggtcca attcatcggg acctcggccc
     3481 aaggcgttcc cgccgctatg ccgtgttccg gcgcgccggt tgcttcgtca taaatccacc
     3541 cacaaattaa acacatccag gtcattgatt gagtcacagc ttaagttgcc ttcgaataga
     3601 atgcaacaat tgtatcgagg cggcgttgct tattcgctta cgaggcaaaa ttccagccct
     3661 atggcaaacc ccacccccac tcctctggac agcccgcaaa tggaaacaga ggacagcgaa
     3721 acctctcccg cctgcgtgat gagcttcaac gccaatgacc ccagcggcgc tggcggcctg
     3781 acggcggaca tcagcgccat tgcatccgcc ggagcccacg ccctggcggt cgtcaccggc
     3841 gcctatgtgc gtgatacggc cgagattttc gatcatgttt cctttgacga cgaggcggtc
     3901 accgagcagg cccgcacggc actggaagac atgccggtcg ccgccatcaa ggtaggtttt
     3961 gtcgggagcc cggaaaccat cagcgcgatc gcggaaattg cgtcggacta tgccgatgtg
     4021 ccgctgattg cctacatgcc caacctgtca tggtgggaag aagaagcgat cgacagctac
     4081 ctcgacgcct ttcgcgaact gatgctgccg caaaccaccg tgctggtggg caaccacagc
     4141 agcctgtggc gctggttgct gccggactgg accggcgaca agagtccgtc ggcacgtgat
     4201 atcgccaagg ccgcttccga gttcggagtg ccgtacacgc tggtgaccgg cgtgcccctg
     4261 ccggaccagt tcattgacaa cgtgctggcc acaccgcagg ccatcctcta cagcgaaaaa
     4321 ttcgagcgtt tcgaggcggt gtttgccggc gccggcgaca cgctgacggc cgccctgacg
     4381 gcactgctgg ccaacggcca caacctgcag gccgccacgg ccgaggccct gacctacctc
     4441 gaccatagcc tggacgccgg ctttcacccc ggcatgggca atatcgtgcc tgaccggctg
     4501 ttctgggccc aggccgacga cgacaccacg gacgctgacg acgaaggcag cgatccggac
     4561 gctccgctgc cccccttgcc cccttttgac ctacccacca atggcaccaa acactagtcc
     4621 cgacacgtcc cgcaatgcag cactgttcga gcgcgccaaa aagctgattc ccggcggcgt
     4681 caattcgccg gtacgcgcct tcaaggccgt cggcggcacc ccacgattcg tgcaacgagc
     4741 ccagggcgcc catttctggg atgccgacgg tcagcgctac atcgactaca tcggctcctg
     4801 gggcccgatg atcctgggcc acggacaccc cgccgtcgtc gaggcggtgc agaaggccgt
     4861 gctcgagggt ttctcctatg gcgcaccg
//
LOCUS       DAIDFG010000237         4840 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_237_length_4840_cov_16.466458, whole genome shotgun sequence.
ACCESSION   DAIDFG010000237 DAIDFG010000000
VERSION     DAIDFG010000237.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_237_length_4840_cov_16.466458"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          857 a         1605 c         1559 g          819 t
ORIGIN      
        1 gtagtcgccg ttgtaagccg gctcgggcca gagcgcgtcg ccgggcttga ggcccttggc
       61 gcgtgcctgc gtgctggtgg ccagcgtgtg aatctgcacg cccgcgtcgt tgtaatagaa
      121 ctcgcggtaa acgtcccagc cctgggtcgc aaacaggttg cagatcgcgt cgccgagcgc
      181 cgcctgccgg ccgtggccca cgtgcagcgg cccggtcggg ttggccgaga caaactcgac
      241 caccacgcgg cgcgtcttgt ccaccggctt gacgccgtac tgcccggccg ccgtcagcac
      301 ctcgcgcacg gtttcctgct tggccgccgg cttgagccgg aagttgatga agcccggccc
      361 ggcgatttcc accgcatcga cccagcgctg gtaagccggc gccgccagca gagacccgcg
      421 cagcccctcg gcgagctggc gcgggttgag cttgagcggt ttggccagct gcatggcggc
      481 cgtggacgcg aagtcgccat gggcggcgac cttgggcgac tcgaaagcgg ccttctggcc
      541 ggcaccgggg gacagcttgt ccagctcagc ggccaggacc gcgagcaatt cttgtttaac
      601 taggagcatg cgctgatttt acgggtgcgg gccggcgcag tttccgcgcg gcctactact
      661 tgccgtagcc gcgcgccaga aacacggcgg ccagcgggat cagcggcagc agatgggcct
      721 gcaccatcac ccgcttgcgc gccttggtga cctggctggc gtcgggcagg cggccggtgg
      781 cgcgcagctc ccggcgccag cgcgcaaaca gccgcgtggg ctggatggac aacagcgcga
      841 tgacgacgaa caaggccagc ttgacgtaga gcagcgggtt ggtgccgtac cagcccgcac
      901 ccttgacacc caggaacacg cgcagcaagc ctgtcagcaa gacggcaccc gccgccacgg
      961 cgtagatcag gtcgatcttg accaggcgct cgaccacggc ggcgttgagc cactcgctgc
     1021 ggcacagcgc ggcctcgctg gcaagaaaca ccaccatggt caggatggcc aggatatggg
     1081 cgtaggcgag cagggcttcg agcgtcatgg aggtctcgcg gctggtccgg tgcttgtgtt
     1141 cagggctgcg cggtgcgctg gccggtccag aacgccggct cgccgtaatt gtgcttgagg
     1201 aagtctatcc acaggcgcac gcgcagcggc aggtgcttgc gctgcgggaa caccgcgtaa
     1261 atcccgttgg gcggcgcgtc gaagtcctgc agcaccgcca ccagcttgcc ggcggcaatt
     1321 tcggcctcga cctcccaggt gctgcgccag gcaatgccgt agccggccat gcaccagtcg
     1381 tgcagcacct ggccgtccga gcagtcgagc ggaccgcccg gtttgagatg gacgacttcg
     1441 tagtcgctgt cacctgccgc gtccgtgagg acgccttcat gttgtcgccc ctgggcgttg
     1501 cgtggaacac gaaacgccca gccccgcgtc tgcgaagcgt cgctggacag cgtcagacag
     1561 tcgaatttcg cgagttcggt ggggtgctgc ggcgtgccgt ggcgctgcag gtagccgggc
     1621 gtggccacac acagtcggcg gttgtcggcc atgcgcacac tgaccagcga ggaatcgggc
     1681 aggtcgccga cgcgcaccgc gcagtcgaaa ccctcacctg ccagatcgac cacgcggtcg
     1741 ctgaggttca gcgaaatcgt gacgtcggca tgcaactggc gaaacaacgg caccagcggc
     1801 gccacatggc ggcgaccgaa cccggccggc gcggtgatgc gcagatgccc gctggccttg
     1861 accccgccgg ccgacacgct ggcctcggcg ttggccacat cgatcaacag gcgctggcag
     1921 tcttccagaa aggcactgcc ctcgtgggtc agcgaaatcc gccgcgtggt acgcaccagc
     1981 agcttgacgc ccagccgttc ttccagcgca tcgagccgcc gccccatgat ggccggcgcc
     2041 acgccctcgg cattggccgc cgcggtcagg ctgccgcgcg tggcgaccga gacgaaagac
     2101 tccatctgtt tgagtttgct catggcccgc gattatgagc acaaaaggtg gcaatggggc
     2161 gttgaaggtc tttattgcgc cgccgactga gcctccatcc ctgagctcgc aacaatcttg
     2221 ccgtcctgca gggcctggat gtccgtctcg ccataaccga gttcctgcag taatcccaag
     2281 gtgtgttcac ccagccgggg cgggttcagg cgtacaccgg ggcgctcgcc gcccagagtc
     2341 aggggcagta gcggcacatg agtggcgcgc ccgtccggca gcgttagcgg cgccaggccg
     2401 ccggtctgct gcaaatgcgg gtcgtcaaac aagtcctcgg gacgtgtgat gggcgcaaac
     2461 ggcaggccgt gctgctcgaa cagcgcgctg agttcggccg ccgttcgcgg cgccagacgt
     2521 tcgcgcagct gcggcagcag ccagtcgcga gcacgcacgc ggtcgttgtt gctgtccagc
     2581 cgcgggtcgg cgcgcaggtc ggcaaacccg aaagcgtcgc aaaacagctg ccactgggtg
     2641 tcgctgacca cggccagaaa gatctgcgcg ccgccctgca cggtgaacac gtcgtaaatg
     2701 ccccaggccg agatgcggcc cggcatgggc gcggccggct ggccggtcac ggcaaactgc
     2761 atcatgtgct gggccaccag gaacacattg ttttcaaaca gggccgcctg cacctcctgg
     2821 cccttgccgg ttttcgcgcg cgcagccagc gcggccatgg cgccgatggc accgaacatg
     2881 ccacccatga tgtcattgac gctggttccg gcacgcagcg gatcgccggg ccggcccgtc
     2941 atgtaggcca ggccgcccat catctgcacc acctcgtcga gcgcggtgcg gtggtcatac
     3001 gggccgggca aaaacccctt gtggctgaca taaatcaggc cttcattcag tctggacagg
     3061 ctggcatagt cgagccccag tttctgcatg gtgccgggct tgaagttctc gctgacgata
     3121 tcggcggtcg ccaccagctt gagcgcaatc tcccggccct gcggcgtctg caggtccagg
     3181 gcgatgcttt tcttgttgcg gttgaacagc gcaaaaaacc cggcgcccga gcccagcaag
     3241 cggcgcgtgt tgtcgccgcc cagcggctcg accttgatga cctccgcgcc cagatcggcc
     3301 agcaccatgc cgcaggtcgg gcccatcacc atgtgggtga attcgatcac gcgcaggcct
     3361 gccaacggca gaggcgtggc ggggccagaa gccgttgggg gcggcgggga gggctcggac
     3421 acagtggaat ccttgaaaga cgatggttga ccgactgcca ggagcctgtc ggacttggga
     3481 atcgtcggct gcaaattatg cggcacccgt caggccccgg aaattaccaa caaatatgtc
     3541 atggataaat cactttttaa tcaattaatc tttgacaaag tatcaaatac agtctagcca
     3601 tggaaaaacc caaaaccggg cgcgccgcgc cacaggctgt tctctttgat gcctacggca
     3661 cgctgttcga cgtgtacagc gtcggcctgc tggcggaaca gctgtttccg ggccagggac
     3721 aggcgctggg cgtgctctgg cgcgacaagc agattgaata cacgcggctg gtgactacca
     3781 gcaatggcgg tgaacactac cggccgttct gggagctgac acgcgccggc ctgcgttacg
     3841 cgtgcaagcg cctgggactg gacctgacgg ccgaacgcga agatcggctg atgaaccagt
     3901 accggcacct gtcggccttt ccggaaaacc gcgaagtgtt gcaggctttg aaaaaacgcg
     3961 gcatcgtcac cggcattctg tccaacggcg atccttcgat gctggacgtg gccgtcaagt
     4021 ccgccgggct cgaggatctg ctcgaccatg tgatcagtgt cgacagcatc cgcaaataca
     4081 agacccaccc cgatgcctat gcgctcgggc cgcaggccac ggggctgccc gttgcgcaga
     4141 tcgcctttgt cagctgcaac agctgggacg cgctggccgc gacctggtac ggctaccaga
     4201 ccctctgggt caaccgttac gccctgccct tcgaggagct gggcacccag ccgtcgcgca
     4261 acggcagcaa tctgcgcgac gtgctgggtt tcttcccggc gggataatcc gtttcactgt
     4321 ttcgagcttc ccttttttcc ttccctttcc ttctttttgc ccttttttga ggttccccca
     4381 tgacgacaac aaccccacgc acaacgctcc acggcctgca ggtggccacg gttctgcaac
     4441 gctttgtcga tgacaaggtc ctgcccggca ccggcgtgga ccgcgccgcc ttctggaaag
     4501 gttttgacgc catcgtggcg gacctggccc cgaaaaacat cgccctgctg gccgagcgcg
     4561 accgcctgca gaccgagatg gacagctggc acaaagccca ccccggcccg attgccgaca
     4621 tgaaggccta ccgcgccttc ctcgagaaaa ccggctacct cgtgccctcg ccgaaaaaag
     4681 tccccatcac cacgggcaat gtcgatgcag aactcgccaa acaggccggc ccgcagctgg
     4741 tggtgcccat cctgaatgcg cgttatgcgc tgaacgcagc caatgcgcgc tggggctcgc
     4801 tgtacgacgc gctgtacggc accgacgtgc tctccgaaga
//
LOCUS       DAIDFG010000238         4723 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_238_length_4774_cov_16.205976, whole genome shotgun sequence.
ACCESSION   DAIDFG010000238 DAIDFG010000000
VERSION     DAIDFG010000238.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_238_length_4774_cov_16.205976"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1131 a         1400 c         1269 g          923 t
ORIGIN      
        1 gtgatctcgg cataacgtcc ttcggcgatt ttcttgccgt agttcaggac gacgcagcgg
       61 tcagtgattc tttcgattac gttcatttca tgctcgatca ggacgacaga cagcctgtca
      121 agctttccac gtacctgcaa aatatcctgc atcaactcgt ctgtttcatc atgcgtcatg
      181 cctgctgagg gctcgtccag aagcaacagg cgcggcccgc tcagcagcgc gcgacaaacc
      241 tcgatgcggc ggcggtcgat catcgtgaag ctatcgaccg gctcgaacag ccgcccgacc
      301 agaggagggc tgaagatctc cagcaaggcc gtcactttct cgacgtaagc ttcgtactcg
      361 gcccgaaacg ccttgcgccg caacagattg aagatcaggc catgctgcat gtgctgatag
      421 tcgccaatga caatattgtc gaaaaccgtg agggacagtg aaaggcggga acgctggaac
      481 gtacgggcga cgccgctgcg atacacctcc tgcggcgtct tgccaatgat gttttcaccg
      541 tcaaggaaaa tgctgccgct gctggctttg tacagcccgg tcaacacatt gaagaaggtg
      601 gtcttgccgg agccgttcgg acccagcagg ccgagtattt ctccctcctg cacagtcatg
      661 gacagctcat cgagcgccgt caggccgcca aaacgcatgg tcagcttgtc aaccgagatc
      721 agggactggc cggccatcat gaatccttcc tggcgaaaaa tatccgtgtc ttgcgcggca
      781 gcagcccctg cggccggaac agcagaatca caatcaccag cgctgcatac aacaggaagc
      841 ggtactcctg aatgaattga agtttttcag gaagaatcaa cacaatcacc gcggccggga
      901 tcaggccggc aatattgccc aggccaccca gaatgacaat cgagagcatc agcagcgaat
      961 caccaaaagt gaagttgttg ggcgcgacaa atgcaatgac catcccgtaa agagcgccgg
     1021 cgatacccgc gaaaaagttg cccatggtga aggcgatgat cttccagcga acgatgtgca
     1081 ggccgaaagt tgatgccgcc gtttcatcgg ttcgcaacac gtccatgctc aggccgaccc
     1141 aggagcgctc cagtgcattg accagggcat agacgcccga gaacatggaa agactgagta
     1201 atgcgtaagt cacataaaac gagatctcca ggccgaacag gctgaaaccc tcgttcaacg
     1261 acaagccgag caatttcatg ccgggcacct gcaggccttg tggcccaccc agcacatcgt
     1321 tgacctcgat gaaggtcttg aacaggatgc cgaatgcgat ggtgaccagc gccgcatagt
     1381 gaccacgtgt tcgcaacacc ggcaagatca gcagcgaacc gataagggcg gcactcaagc
     1441 cagagatcaa gatgacgagg acgtggggaa tggcggtgtg ggaagcaagt acggccgtgg
     1501 cgtaactgcc aatgccgaaa aatgcggcac ctgcaaagtt ggccacgccc gcgtagccga
     1561 actgcaccgt caaaccgaga caggccgtgg cgtacagcaa cacgccgcac agcatcagca
     1621 acacgaagtg tgagctgtgg aaagccgcaa tcagggccag gccacagcca actgccaacc
     1681 atcgggacaa gcccgggcgc cgcgcggccg actcctcgac cagcaacgtc acgccgaatc
     1741 ggcgactcaa catcactgcc aggatcgcgg caaccagcaa aatggcaatg ccgatttggc
     1801 tctctgcatg caggaacaac catgcataga cggtcaggac cagcgccgcg atgctgagca
     1861 tcacggcaga tttcgcgggg gtcatggatt cttgtgtttt catggtacta aacgcgttcg
     1921 ctcgactttt cggcgatcag accagtcggc ttccaggcca taaaaatgat gacgacggcg
     1981 aaggcaaaaa catccttgta agagcttgga atatcgggaa tcagtgcggg caacatggcg
     2041 ctgccaatca cctgcaaggc agcaaaaaga aatccgccaa tgatggcgcc atagatattg
     2101 cccagccccc ccagaatggc cgcggcaaac ccgatgaccc ccagcagcaa gccgacattg
     2161 aagttgactt cgttgtagta caggccattc atcacgccgg cgagcgcagc caggccagac
     2221 cccagtgcaa aggtgatcag caccaccgcc tcgaagttga tgcccatcaa gcgagcggtc
     2281 tccccgtctt gcgccacggc acgaatcgcc atgccgagtt tggtgcgcgt gatgaggagg
     2341 tgtacccctg caatggcgag caggccgctg gccagcaaaa tgaggttgtc aattcgcaat
     2401 gaaagcccgc ccagctcaac catggaggta ggcagcaatg caggaaacgg ttttgggttg
     2461 gatccgtcag gatagaacag acgaaccgcc tcgcgcatca cggtgccgag catcagcgtc
     2521 gccagcagcg tgttgagcgg tggcgcatga cgcagcggca ggatcaggaa cttggcgatg
     2581 ccggctccca gcaaaccagt cacggcgacc gcaaaaacaa tcaccgcgag cagctgtagt
     2641 gccggagaag ccacgccgat tttcatcatc acgatgaacg cggtgagccc ggcgaaggcc
     2701 cccaccatca aggtctcccc gtgcgagaac ttgacaacgt ccagaacccc gaaaaacagg
     2761 gtaaagccaa cagcgaccat ggcgtaaata acgcccagca tcaggccgtt gaagatgtat
     2821 tgactaactt cactcatcag gtttcctgaa acgattgaag attaccctcc cgcgccgccg
     2881 atggcgtgca ggagggtatc cagggaatta cagaccggcc agcttgcgct tgcctttgcc
     2941 gtagctgctg tcttcccaaa ccacccatgc accgttttcc accacgtact tggtgatgag
     3001 cggaacgatg ttctggcgat gatcgtcaaa ggtcaccttg ccaatgatgg tgtccacatc
     3061 cttggtactg ttcagggtgt cacgcacttt tttgcgatcc ggaccgactt tctccaccgc
     3121 atccatgatc agattggcgg cggcaaacgc aaagggacca taagcctctg ccgattcact
     3181 gtatttttgc tgcgcgtatt tgctggtgaa gaacaacccg cccggcagct tctcccatgg
     3241 cgccccttcg ataaacgaaa gcgtgccttc agccagttcc ttgcctgcac cttgaatata
     3301 ggcgtcagac ttgatcccgg aggtgccctg gagttgagcc ttgatgccca gcttttccat
     3361 ctgggtccga atccgtacgc cgagcggtgt cagtccgccg aagtaaatga cgtccggatt
     3421 caactcacga atcttggtca gttcggtggt gaagtcctgt tggtcagccg tcacaccgaa
     3481 agtgcccagg accgtaccac cacctttttt caggaactcg ctgaagtatt tgttgtgccc
     3541 tttgccgtag tccgttgtgt catgaataat ggcccatttt ttgtacccca gtccggccat
     3601 gaacttgtag gcaatctcgt tctgattgat catcgtgccg ttgacacggt gaacctcctt
     3661 gaagttgttg ccgtaggtga cctctggcaa aaccgcaccc cacaccacag cgggcatgcc
     3721 aaaacggttg tacacgccag ccgtggccat ggcgactgcg gagcaatagt gcgtgacgcc
     3781 cgccacgata gttttgtctg cggctacctt ggtcgccacc tgcacaccca cgttgggttt
     3841 gcactcgtcg tcctgcgcca ccaattcata ggtgtatttg gacttcgggt cctgattgcg
     3901 caggcgtacc gccaattctg cagaattgcg cccccccaga cccatattgg agaccccacc
     3961 ggtcaacgga ccgacaaagg cgattttcac cacctcttta gccatgactg gactggccag
     4021 aagagccaga ctcatggatg tcagcagcgc cgttcctgcc aatattgtgt ttcgcctacg
     4081 catggtcgtt tcctttcggg tggagggatg gttcaaatca agtaacagga ttctgattga
     4141 gagcactttg aatacagttc aagtcacttg aatttgcatg agcatggttt atgccagata
     4201 tagttctcac tcaacaccag aggaaacccg aacccgtcca acaccacgct cctcaggagg
     4261 caaggcctcc atgaatcatt cgacgggatc cgccgtgtta cagacctcga gtcgatcaac
     4321 aaatccgaaa aaggcgaatc gccagctcga cgcaccgtca cggtgcacaa ggaatacccc
     4381 gctttggtgc agttcattca tcaaggccac tgaactgggg acgccaggcc accagctcgt
     4441 tccatcgtgg acgctgtgct ttccgggctc gaggggccca cgccaatctt gccgccatcg
     4501 gcaccgctgc ccttttcggc agagacgact catcgggtgt tgatagactt gagccaatga
     4561 ccgacgccgt taagacccat actccaatga tgacccagta cctgggcctg aaggcggact
     4621 acccagacac cctgctgctg taccgcatgg gtgactttta cgaattgttt tatgccgacg
     4681 ccgaaaaagc cgcgcgcttg ctcgacatca cgctgacccg gcg
//
LOCUS       DAIDFG010000239         4766 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_239_length_4766_cov_16.755041, whole genome shotgun sequence.
ACCESSION   DAIDFG010000239 DAIDFG010000000
VERSION     DAIDFG010000239.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_239_length_4766_cov_16.755041"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          883 a         1544 c         1470 g          869 t
ORIGIN      
        1 gctgcatcgc gttgttggct tcgatggtgc cgtgcatgcc cagcatgccg aggaattttt
       61 tgtcggtggc cgggtaggcg cccaggccca tcagcgtgtt ggtgcagggg taacccagca
      121 tgtccaccag cgtgcgcagc tcctgcgagg cattgctcag caacacgccg ccgccggtgt
      181 agatgtaggg gcgcttggcc gtcatcaaca actgcagcgc cttgcggatc tgcccgccat
      241 ggcccttgcg caccgggttg taggaacgca tttcgacggt ctgggggtaa ccgctgtagg
      301 acgtcttgtt gaacgagacg tccttgggga tgtccaccac caccggcccg ggccggccgc
      361 tgcgtgcaat gtggaaggcc ttcttcagcg tctcggccat ctgccgcaca tccttgacca
      421 ggaagttgtg cttgacgatg ggacgcgtga tgccgaccgt gtcgcactcc tggaaggcat
      481 ccaggccaat cgcgtgcgtg ggcacctggc cggtgatgat caccatcggg atgctgtcca
      541 tgtaggccgt cgcaatcccg gtgacggcat tggtcacgcc ggggccggaa gtgaccaagg
      601 ccacgcccac ctcgccggtg gcgcgggcgt agccgtcagc cgcatgcacg gccgcctgct
      661 cgtggcgcac caggacgtgc tggatggtgt cctgcttgta aaaagcgtcg tagatatgca
      721 ggacggcgcc gccggggtaa ccccagacgt gcttgacgcc ttcggcctgc agggccttga
      781 tgaggatttc cgcgccgcgg agttcttgcg gggttgttgg ggcagaagct gccgccgctg
      841 ccgaagcgag ttcggccttt gtgatttcca tgatgaacct ttgtgaattt ctctaacgaa
      901 ataccttggg tgcctcttcg cccactcttg tggagtagcg tcgagacttg aggccaaaac
      961 catggacgga aacatatccc gccgggtcat gacccgtttg ctgtttgaat tgcaatgcat
     1021 attatggcat caggcggcgc gtggccagcg gagcgtgcaa aaaagtccgg ccggcaccat
     1081 gcttgatgcg ataattttgc agctttggac gcttcactgc gcctgaaaaa cacaacaatg
     1141 acgacgctgg gacgaatcgg gatgcatccc cttggcaact gaacaagaac tctccgattt
     1201 cctgaaaaac gttgaaaagc gcgctttcaa gcgcagcgtc taccatgtcc gcgacgacga
     1261 ggcggcactc gacatcgttc aggacagcat gatgaagctg gcccaccatt acggagacaa
     1321 gcctgcggcc gaattgccca tgctgttcca gcgcatcctg tccaacacca cgctggactg
     1381 gttccggcgc cgcaagacgc gcaacgccct gttttccaac atgagcgact ttgaatccgc
     1441 cggggacgat ggcgatttcg atctgcttga aactttggag tcctcgacca actcagaggg
     1501 aacagaaagc gcccaggaag ccaccgaacg ggcgcaaatc ttgcgacaga ttgaggtcga
     1561 gatcaaggaa ctgccgggcc gtcaacggga agcctttctg atgcgttact gggaggaaat
     1621 ggatgttgct gaaacagctg ccgccatggg ttgctccgag ggcagtgtga aaacgcattg
     1681 ttcgagagcc gtccacgcac tcagcaaggc tttggccgcc aagggaatca aactatgaac
     1741 catacactgc aaaaacgcac cgagatcctg caggaccgct atggcctgaa aaccgcttcc
     1801 tacctttccg ctggcgcggc ggacctgccg tacgacattt ccgagcggct gcgggccgcc
     1861 cgtgcgcagg ccgtggccgc ccgcaaggtg gccagggttc aaacctctcc ggtcaccgca
     1921 gccaacggca caggttcggt gctgacctgg ggatccggcg agaggttcgg cctgtggtac
     1981 cggatcggat cggtgctgcc cctgatcgcc ctggtcgccg gcctgctggt catcaactcc
     2041 ctgcaaaccg acagcttcgt cgaggaactg gccgaggtcg attccgcctt gctgaccgac
     2101 gacctgccgc ccgacgcctt ttccgatccc ggatttgtgc aattcctcaa ggttagccgc
     2161 taggtccggg tttgcccttg cgcagaaaga cgtctacttc gatgcctgtg accctgtccc
     2221 gccctcctgc gcgtctgcaa accgtacgcg gtaccttgat ccatgcccgc ctgtcccacg
     2281 cgctgccggg atgggtgggg ctgctctgcc tgttcctggg tgccctggcg accggagccc
     2341 acgcccagac gcccgtctcc gcagcagcgc cagcgcggcc agccaaaacc gtgatcgccc
     2401 aggccgcgtt ttccagtccc atctggaccg agttgagtcc ggtggaacgc gagatcctca
     2461 agccgctggc gtccacctgg aacacactga gccagaccca caagcgcaaa tggcttcaga
     2521 tgaccaaaag ttatccggcg ctttccaccg aagaacaggt caagatgcag gggcggatga
     2581 aggcctgggt ggcgctcagc ccccagcagc gcgcccaggc gcggctgaat tttgcaaaaa
     2641 ccaaagagct gtccaaagag ctcaccgctg aagaaaaaca ggccaagtgg caggcctacc
     2701 aggccctcag cgccgaggaa aagcgcaaac tggccgccaa ggcaccgccc aggccggcgg
     2761 gcgccgcccc ggcggccaaa ccggttgcag cgcaaaagct ggcgacggtg ccttcgcgcg
     2821 ccgacaagcc aaaggccaaa ctagcgacta aaatcgtggt gacgccaccc ccggccgggc
     2881 agccggacct cctgtccgcc accgcgccgc ctgccacgcc tggcgcggca ccggaaaaac
     2941 cgtaaccagc aaggcccggc ttccgtgccg gcccgacacg atccgcctgc gggtctggct
     3001 gcggctcctt cgggcgcatt taaccgacct gacctgaacg cgaccacaaa ttgacagccc
     3061 tgcaagccaa aagccttccc actccctccc tgcgtcggcg catggcctgc tggctgtatg
     3121 agggcatgct gatgttcggc gtggtgttca tcgctgacta cctgttcagc accctgagcc
     3181 agacgcgcaa cgccatggac aaccgcaatg cccagcaggc cttcctgttt gtcgtgttcg
     3241 gcatctactt cgtctggttc tgggccaaag gccagaccct ggccatgaag acctggaaca
     3301 tccgcctgac cgacctgcag ggacaggccg tcacccagaa gcgcgccttc ctgcgctaca
     3361 ccctgagctg gctctggttt ttgccgccgc tggcggtgag ctggctgctg gggctggacg
     3421 tgcggcaggc gctcgtgctg gccggcgggt ggatcgccat ctgggcaatc ctgtcgcgct
     3481 ttcacccgga ccggcagttc tggcacgatg cgctggccgg cacccggctg gtatccagcc
     3541 tgcccgctgg cgcgcctgcc cgggccgcct gaagcctcgc ggcgccctcg ccatgcaaga
     3601 gccgcatcac accgtcacag agctgcgcga caatcggccc atgcccgaac acgtatccac
     3661 cgcctccccc cctgcgcttc aaggccaccc ggcagccacg cctcccgcca atccccaaaa
     3721 agaccgcaaa ggcatcaacc gagtctggca tgccttcggc tattctgtgg cgggcttgcg
     3781 cgccggctgg caggaaacgg cgtttcgcca ggaagccatt gccgcgctgg tgctgcttcc
     3841 cgccgccgtc tggctgggcc gcagctgggt ggagaccgcc ttgctggctg gctcggtgat
     3901 cctggtgatg atcatcgagc tgctcaacac cggcatagaa accgcgatcg accgcatcgg
     3961 cccggaatgg cacgacctgt ccaaacgcgc caaggacatg ggaagtgccg ccgtgttgct
     4021 gagcctgctg ctgtgcgcgg gcatctgggt catggcattg tttcagaggt ttttcgcatg
     4081 aagccggctt tttcggtctg cgtttattgc ggctcgcgac cgggcgcgga ccccggattt
     4141 tccgaaaccg cacgccaggt cggccagtgg ataggcgagc atggcggcca gctggtgtat
     4201 ggcggcgggc gcaacgggct catgggcatc gtcgcggatg ccaccctggc cgcgggcggg
     4261 cgcgtcgtcg gcgtcatccc gaaggcgctg gtggagaagg aatgggccca caccggctgc
     4321 accgaactgc acatcgtgga caccatgcac gagcgcaagc gcatcatggc cgaacacgcc
     4381 gacgccttcc tgtcattgcc aggcggcata ggcacgctgg aagagttttt tgaagtgtgg
     4441 acctggcgcc agctgggcta ccacgccaaa ccggtcggcc tgctgaacct gaacggctat
     4501 tacaaccact tgctggcttt tttggcgacc tcggtgacca gtggtttcat gaacgactgg
     4561 cagatggacc tgattcacgt cgccaatgat gccgaacagt tgctggagca actcgtgcaa
     4621 tcagcgggcg tggcgcccga cgcgctgcgt ctggaccaga tttaaggcag tcgctccgag
     4681 ctggccattg gggccctgtc ttcaccagcc agggccgcgg atctggcttt gccagtccgc
     4741 aggcgcagcg gccccctcgg ggggca
//
LOCUS       DAIDFG010000240         4753 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_240_length_4753_cov_21.538527, whole genome shotgun sequence.
ACCESSION   DAIDFG010000240 DAIDFG010000000
VERSION     DAIDFG010000240.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_240_length_4753_cov_21.538527"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          800 a         1534 c         1510 g          909 t
ORIGIN      
        1 gcgccaacgt gatgctgctt tcaagtgcat ccagcaagac cgcctatggc acagccttcc
       61 agctggccca gcgcccgggc atcgaagtca tcgggctgac gtcgccggcg aatgtggctt
      121 tttgcgaaag cctgggttgt tacagccggg tgctgactta tgaccagctc gaccagatcg
      181 cagcggatgc agcctgcatc tatgtggact ttgccggcaa cgccgggctg cgccttgcca
      241 tccacacccg ctttgcgaat ctgaaataca gctgctccat aggtggtacc catgtggccg
      301 agctgggcgg cgcccgggat ctgccgggtc cgcgcgccac cttgtttttt gcaccggcgc
      361 aaatcaagaa gcgcagcgcc gactggggcg cagaaggcct gggccagcgc ttggtgcagg
      421 cctggcagac gttcagccag cgtgcgacac aagcgccttc gccctggctg atcacgcagc
      481 accaccgggg tgccgacgcc gtcaccgcgg cctaccagca ggtgctggcc ggcaagggcg
      541 atccgcgcgt tgggcatatc ctgacactgt gacgcggctg ggggcaatgg cggtttgcta
      601 ttatttcaat agctgctttc gcacatccat caagggctag agccactttt tacttgaaat
      661 ttcggggcag atccggatta caacgacaat gagggcatga ccgaacaacc cctggctgca
      721 ccgactcccg acgccgtcca cgcctacgaa aaactcacgc ccgacgtggt actggacgca
      781 ctggccagcg tgggcctgta tggcgacgga cgcctgatgg cgctctccag ttatgaaaac
      841 cgcgtctacc aggtccacct ggaggcgccg gcgggcgcgg gctctgcggc gggtgacatc
      901 gtggtcgtca agttctaccg gcccggccgc tggagcgatg tgcagatcct cgaagaacat
      961 gaatttgccg ccgaactgat ggctgcggaa attcctgtcg ttggcgccct ggcgctgggt
     1021 ggcagcaccc tgcaccactt caacggtttc agcttcagcg tgtcgccgcg ccgcggcggc
     1081 cgccggcccg agctggagga cctcagcgtg ctcgaatgga ttggccgctt cctcgcgcgc
     1141 atccacacgg tcggcagcgc caagcccttc aaacaccggc cggcgctgaa cctgcagacc
     1201 tttgcctacg ccagccgcga cctgctgctg gcgggcaacc acctgccgct ggacatggcg
     1261 tcgcgctggc tcaaggcgtt cgaagaagcg atgaacgtgg cgcaggccgt gtttgaaagc
     1321 gtcggcgatg tccgcacgct gcgcttgcac ggcgactgcc accccggcaa catcctgtgg
     1381 acgcccgagg gcctggaagg cgctggcccg cactttgtcg acctcgacga cgcacgcagc
     1441 ggcccggccg tgcaagacct ctggatgctg ctgtccggcg aacgcgcgca gtgcctgcaa
     1501 cagctcggcg ccctggtgga cgggtacgag gagttccgcg agttcgatcg ccgcgagctg
     1561 gcgctgatcg aaccgctgcg caccctgcgg ctggtgcatt acagtgcctg gctggcccag
     1621 cgctggcacg acccgatttt tccgatcaac ttcccctggt tcggctccag cgactactgg
     1681 aaaggccagg tcgacatgct ggaagaacag acggaagcca tgcaggaagc gccgctagtg
     1741 gcgtaatttt tggttgacat tggttccaat atattggaaa ctttttccac catgaaagaa
     1801 aagtcttcgg acagcctaaa tacacgcatt gcacagcgcg tgcgtgacct gcgcgctgcg
     1861 cgcggcctct cgctggacga gctggcgacg cgctgtggcg tcagccgctc catgctttcg
     1921 ctgatcgagc gaggagaaag cagccccacg gcggttgtgc tggagaaact ggcgaccgga
     1981 ctgaatgtgc ctcttgcatc gctttttgaa gtcccccagc catcggataa cccggtggcg
     2041 cgctgggccg accaaccctc ctggagcgac ccgcattccg gttatgtacg ccgaaacgta
     2101 tcgcccagca ctagcgagtc acctatccag atcgtggagg tgtcgtttcc acccaaagcg
     2161 cgcgtggctt atgaaaccgg cgcgcgggaa gtgcaaatcc accaacaggt gtgggtactg
     2221 gagggcgcca tcgatgtcac cgtcggcgac gcactgcacc agctcggcac cggcgactgc
     2281 ctggcgttgg tgctggaccg gcctgtgact tatcacaacc cgactcgcaa gaccgcgcgt
     2341 tacgccgtgg tgatcaccac cactcccacc tcccggagat gacatgccca caaaaaactc
     2401 tgttgaccac ccccgcacct accgcctcga aaccgtgacc gaaggccagc tacagtcgct
     2461 gtccgacctg ctgatcgatt gtgtggacgg tggcgcctca gtaagtttca tgcatcccct
     2521 gtccatggac aaggcgctgg cgttctggcg cggtgtggcg gacagctcag cccggggaga
     2581 acgggcgctg ctggtggccg aagatgccgc aggcatcgtg ggtaccgtgc aggtgattct
     2641 ggaccagccc gagaatcagc cgcaccgcgc cgacgtggca aaaatgctgg tgcatcgccg
     2701 ggcgcggcgc cagggcttgg gtgaagcgct gatgcaggct gccgagcagg tcgcgcgcga
     2761 atacggcaaa accctgctgg tgctggacac tgccagcgac gatgccgagc ggctttatgc
     2821 gcggctgggc tggcaacgct gcggagtgat tccgggttac gcgcttttcc cgaatggtga
     2881 gccgtgcgat acgacttact tctaccgctc gctcaaccac tgaatttggc cgagcgctgc
     2941 ccatgccaga aagcaaaacg gaatccgctc cagaccgacc actctggaag acctttctgg
     3001 tcttcctcag cccgctggtc ctgaccaaca tgttgcaggc cctgtcgggc acgctcaaca
     3061 acatctatgt ggggcagatg ctgggtgtcg ctgcactggc tgccgtggcc agcttttttc
     3121 ccctgctgct gtttttcatt tccttcgtga tcggcctggg ggcaggtgcg tccgttctca
     3181 tcggccaggc ctggggcgcc agagatactg aaaaggtgcg tgccattgcc ggcaccgtgc
     3241 tttgcgcagg ctttttgctt ggcgtggtgg tcgggctgtg cggcatgttg ctggccgaac
     3301 ccatcatgcg cctgctgggt acgccggcgg atgtactgcc ccaggccatc gcctatgccc
     3361 gggtcatgat ggctgccttg ccgctgatgt ttgtctccat gctgacggcg gccgtgctgc
     3421 gtggcgtggg cgataccgtc acccccctgt tgtcactggt ggtgtcttcg accatcattc
     3481 tcatgctgac cccggcactg atccagggct ggctcggcct gccggcactg ggcgtgaccg
     3541 gcggcgcctg ggcgacgctg gtgtctgcgg ccattgccct gctctgggtg gcccggcacc
     3601 tgcgacggcg cggtcgcacg ctcgcacccg acagggattt ctggcggcat gtacgcctgg
     3661 agaaaaaatt cctgaaggcc gtcgtgcggc tgggctttcc caccgggctg ttcttcatca
     3721 ccggctcggt ggcggacctc gccctgctgc gcctggtcaa tggctacggc tcccatgcca
     3781 cggccgcctg gggagccgtc agccaggtca tggcctatgt ccagtttccg gccatctcga
     3841 ttgcgattgc ggcgtcggtg ctggcggcgc aggccatagg cgcagggcgt ctgcaccaga
     3901 tcgacagcgt gacacgcgtc gggctgggca tgaacatcgc catcaccggc ggtctggcag
     3961 tgggcgtgct ggtgtttgcg cggaccatcg tcgggctgtt catcaccgac ccggcggtcg
     4021 tcacgctggc ggccggactg ctgcacatca ccgtgtgggc cagcctgatt ttcggcctgg
     4081 ccagtgtgtt cacgtccgtc atgcgcgcca gcggaacggt gctggtgccc acgctcatct
     4141 cgctgggctg cctgagcctg ttgctgttcc cgctgggctg ggcgttcagc cagaccttcg
     4201 ggctgcgcgg catctggatg agttacccgg ccacctacgc ggtggcactg ctgctgcaga
     4261 cggcctactt cttcgtggtc tggaagaaaa aacccatcct caaaatggtg tgagtggatc
     4321 gcgccccgaa cgctaacgaa tgcgcggcgg cgcctgcaac tgctcgggcg tggtgaagta
     4381 ggcggcggtg ctgcccgcac gcgccttcgc ttgcgccagt tcgtgccgtg ccacggtgcg
     4441 caaatgtact tcgtcgggcc cgtccatcag gtgcagggcc cggccccagg tccagaaata
     4501 agccagcggg gtatccggtg acaggcccat ggcaccgaag gcctgcatgg cccggtccac
     4561 cacccgcgtc tgcaaccgcg cagccaccac cttgatggcg gccacgcgcg cgcgcagttg
     4621 tgccgcgtcg tccttgcctt cgtccagcgc ccaggccgca tgaagaatca gcagacgcgc
     4681 ctggtcgatc tcgatgcgcg attcggcgat ccattcctgc acgttggaaa aatccgaaag
     4741 gtatttgcca aag
//
LOCUS       DAIDFG010000241         4743 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_241_length_4743_cov_19.549275, whole genome shotgun sequence.
ACCESSION   DAIDFG010000241 DAIDFG010000000
VERSION     DAIDFG010000241.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4743)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_241_length_4743_cov_19.549275"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          899 a         1550 c         1482 g          812 t
ORIGIN      
        1 tggtgacttt cttttggcga cgcaaaagaa agttacttgc cgccgggcaa cccccggctt
       61 gctggcaaac ccccggcgca tgaggaaaag gcagcttcta cagacctgaa gcacaacggc
      121 aaagcgccgt tggcaatcaa accggataaa tcagcgagtt gagaatcatc tcgctgtcgt
      181 acacacacag ccggcgcctg aacttcaggc cctcgcccgt ccgcacgatc tcgtcggcat
      241 aacgccccac gttgtagacc tcgctgacac ctgccggctt ggtgcgaaac accgcgtagt
      301 tcgcctgcgc cgtgatgaca tcgccctcaa ccgacaagac ctgcgccggc gcgatgacgt
      361 gccgcatgta gtacggcccg tggtaaatcg tctgcgtgat gccgtagacg cggtctttca
      421 tcatgccgcg gctttccagg tccatcaggg ccagcggcag gccgcggtcg aaattttcgc
      481 gtgcctgcac ggtgtaacgg ccgtcctcga ggaaaaactc cggccaggca tcaaaacgtt
      541 tctcgtccag cacggccgca taagccgcgt tgagctggtc gatatcgaac tgaagcacca
      601 gccgttccag cgtcgcactg ctcatggcgc cacctccgtc atcactttct tccagtaggc
      661 atacatgctg cggatcagcg tttcggtcac catatgttcg gtcgcctcgg tgccggtacc
      721 gcccagttca cacagcgtcg aacgccattc cccgccctgg cggaaaccgt cctggctgaa
      781 ctcgatgacc tcgccgtcgt cggcgctgac aaagcccgcc ggcccgaaca ggttggcctg
      841 gcgcaggcgc cgccgcgtca tctcgggcgt gtcgtcggca aaaccgaaat gggtccagac
      901 aaaatcaaac tcgccctcgc cgcgcggcac gatgtggcgc gtgctcagcg agttgacctg
      961 ctgctggatg atgaggctgg gaaacagcgt gatcatggtc acggtcggcg tgatgtcggt
     1021 gccggggttc gagggatcat cgaccttcca ccaggcttcc ggcgccacgt cgagcaggcg
     1081 gtcgtcgtgc agcgccatgt tggccttgaa cgaggtcacg ccctcggtca cggcagcgtt
     1141 ctgcccgccc tcgttgcgcc gcgagatcat cactgcgtgg cggccgtgtg cgtccatcac
     1201 catgcggctt ttctggtcgg cgcgccacag gccgaaagtg acaaaccagg tgtgcagcag
     1261 accggggtgg taggggtcct tgatgttttc catcatcagc ttccagttgc ccggaatgcg
     1321 ctggcggttg tagcccagca aggtgagctg ccggcctttg aagatgcggt ccagccaggg
     1381 catcacctgg gggcccaggt aatcttccag cggctcggtc gcctcgctga aggtcgcaaa
     1441 caccagcccg tgcagcacgg ccacacgcag cttggtcagg ccgtggtctt tcagatcgaa
     1501 cccttcgggc atgccgccgt tgatgcagtc gccctccttg acgccgtcct tgaagggcaa
     1561 cccggcgagg tcgccattga gcttgtaggt ccactggtgg taggggcaga caaaactgcg
     1621 ggcattgccc tgcccgttct ggcaaaagcg cacgccgcga tgggcgcagc ggttttcgac
     1681 cacgcggatg ccgtggtcgg tttcccggtc cctgggcgcc acccggtcgc gcaccatcac
     1741 gacctggcgt tcaccgacgt ggcttaattt gaagtcgccg agattgggaa tctcgacttc
     1801 gagcccgaca taacaccagt gcgggccgta gaaaatcttc tcgagttcgc gctggtaaag
     1861 ctgggcgtcg gtgtaggccc agaaggggac gcggctggag cctggttcgc tccagcggga
     1921 aagggattga tcagccatca tgcaagcatc ttcccgaccg gctgtccggc ctgtggttca
     1981 tccgataatc ataagcttac ttatcaattc cggaaaacag ggtttatgcg gatccgctgt
     2041 tgcgccgcgc ctgcggctgg tacatggcct cgatctcctg cgcaaaacgc ttcatcaggt
     2101 tgttgcgctt gagcttgagc gtcggggtca tcagcgcgtt ttcgaccgtc cacggctccg
     2161 tcgtcagcag cacggcgcgc ggcatggcgt aacgcgcaaa attcgcggtc tgggccgcca
     2221 tgcgcttgag cgcagcctgg tgcaccgccg gcaggttcag gctggccgcg tcatccgggt
     2281 cgaccccgag cgcctcggcc agccggcgcc attcggccgg gttcaccacc gccacgcagg
     2341 cgatgaaagg ctggttctcg ccgaccacaa acacctgtgc gaacagggga tcgaccgcga
     2401 tcgccagttc caggtcgccc ggcggcacct tctcgccggt cgaggtgacg atgatttcct
     2461 tgatgcggcc catgatgcgg atgcgcccgt ccgtgatgtc cgcctggtcg ccggtggaca
     2521 gccagccatc gggcgtcagc acccgcgtcg tgtcttcggg ccgtttccag tagccccgca
     2581 tcaccgatgg gccgcgcacc tgcagttcgc ggttctcgcc caagcgcacc tccacccccg
     2641 gcaaggcgcg gcccacggtg gccgggtcgt tgtcttccac gccgttggcg gccaccaccg
     2701 gcgaggtttc cgtcatgccg taaccctgca gcagcggcag gcccagcccc aggaagcagc
     2761 gggccaccgc gtgtgacagc ggcgcaccgc cgctgacagc cacccgcacc cgcccgccaa
     2821 actgcgcacg cagggtgcgc gccaccaggc ggtccagcaa gggcctcagc agcgggtcca
     2881 gccaggccca gcgactcccc tcgcccgcgg cgagcggcag cccctgcgac cggcaaaaac
     2941 gccgccagcc cacggcctgg gtggcctcaa acaaacgggt cttcaatggc gaggtggcca
     3001 gcgtttcctg cagtttggca aacacccgct cgtagatgcg gggcaccgag atcagcaccg
     3061 tggggcgcac ggttttcagg tcttcggcca gcaaggacac cgaacgcgca tagaccacgc
     3121 agcttccgga cgccatcggc aggtagtagc ccgcggtgcg ttcaaaggtg tgggacagcg
     3181 gcaaaaacga aaggaacaca tcgtccagcg tggggaccac gcgcgccagg atggcctgca
     3241 cattggccat cacgttgcgg tgcgtgagca tcacgccctt gggtttgccg gtggtgcctg
     3301 atgtatagac gatggcggcc aggtcgtccc cggatggcgg cacaaaagcg ccatccgcgc
     3361 cctgcccggc ggccagccag tcggccagcg aacacacggg cacgtccgcg ccgcctgcat
     3421 cgggtgccac ggcttcacca ctgaccacga cgcgcctgag cgccggcatg gccacaccca
     3481 gcgctctgat cgcatgccac tgcgccaggg acgagaccat gagcagcgcg gcctcgcagt
     3541 ccgccaggat gtaggcaatg ctgcccgggt tgtccagcgc gtggagcggc accggcacat
     3601 ggccgagcgc cagggccgcc tggtcgaggc tgacggcatc cagtccgttg ggaagcagga
     3661 tggcaatgct ggcatggttc ggcaaacgca tggctgccag cgcctggccc cattgggcaa
     3721 aacgctcgcc ggcctgcgcc caggtggtgg cgacccagcg gccggcgccg gcatcaaatt
     3781 cgcgataggc ctcggcttgc gggctgtggg ccacacgaaa ggccagcagc tcggggagcg
     3841 tacggacggt ggaaaaatcg atctcggtga cagtcatggg cagcttgttg gaaacgggga
     3901 gttctccaag gcaatgtgca ccgcgcagac gcgcccgccc tgccctgggt caatacagcg
     3961 ggtaaaaaaa cccgtcagtg ggccgattgc cgggcagacc ggctggcttt ccaggcgccg
     4021 tacaacacaa acagcccggg aatcaggatc atggcccaga tgatcttgct caggttggcc
     4081 tgcacccacg gcaggttgcc gaagaaataa ccggccgtgc acaggcccac cacccagatc
     4141 agcgcgccga tcacgttgta ggcggtgaac ttggtgcggc ccatctcggc cacgcccgcc
     4201 acaaaaggcg cgaacgtgcg gatgaaaggc atgaagcgcg caatgatgat agtgacgccg
     4261 ccataacgct cgtaaaactc gtgcgcctgg ttgaacgcgc gcttgttgaa aaaccgcgat
     4321 tgctcccact ggaacacctt gggcccgaag tgccggccta tcgaatagtt gcactggtcg
     4381 cccaggatgg ctgccaccag catcacggcg atggccatcg gcaggctcag caggcccacc
     4441 ccgcagaggg cgcccaccac gaacaacagg gaatcgccgg gcaggaaagg catgacgacc
     4501 acgccggttt ccacaaaaat gatgaggaag agcaaggcat aaacccaggt tccgtagctt
     4561 tgtgtaaaag cctccaggtg tttgtccaca tgaaggatga agtccatcag aaaagcaagt
     4621 agttccatgg gggtgatttt gacatggtgg tttgcatcga cccccttcag tctgcctgct
     4681 ggccgggact cgccccggcg ggcgactcac tttctcttgc gtcgccaaga gaaagtaagc
     4741 aaa
//
LOCUS       DAIDFG010000242         4715 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_242_length_4715_cov_17.736910, whole genome shotgun sequence.
ACCESSION   DAIDFG010000242 DAIDFG010000000
VERSION     DAIDFG010000242.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_242_length_4715_cov_17.736910"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          908 a         1438 c         1492 g          877 t
ORIGIN      
        1 tgctcgatgg ctcggcgggg gttgatgccc agggtcttga gttcgatctg caccacgggc
       61 acgccgttga tcagcagaac gacatcgaag cggtggtggc tgtaatcggt gttgattcgc
      121 agttggttga caacctcgaa ggtgtttttg caccagtcgt cgatattgac cagcgtgtag
      181 ttcagcggcg tgccgtctgc gcggatgaag ctggtgcgcg tgcgcagggt ctgtgcggcg
      241 gtgaagacat cgggtgtggt gatctcttcg agcaggcgag cgaactcctc gtcggtgagt
      301 ttgacgcggt tgagttcctg gaacttgtcg cgaaagttgg cctcgagcgc ggcgcggtcg
      361 cgaatgtcgg gacggtatcc gtatttcagg ccctgcaact tgccgatgaa gccgtattcg
      421 atctcaccct ctttaacgct gtggcggagc gcgccagggg attggtcgta gattgacgtg
      481 gatgaaggca tagctaaaag atcgcgcttt tcgactgaac ggggtgaaac cggctaggtt
      541 cgcggtaacg gaatggggaa tcaataacct tcagagcata tcccctgtcg tgtaggaaaa
      601 ggatcgcacc gtccagcagg ggtgactgct gtaaatcctg cagcaacccc aggcgcctgg
      661 acttgtcgtc ggctgatttg ataagcatgc tcccctcccc ttcggaattc ttatggtcag
      721 actttacccg aggggcgtgg cgaccaggcc agtgggagca tggacacatc agtcgctgca
      781 gttcaatcgc cccgacgcgc ttcaaatgct ggctgatggg tgccagtggc gcggacggtg
      841 cgaaagaatt ctttaattgg gtgaagtcac attcaagcaa tctttaatac gaagcgcacg
      901 catgaaagca ggtttgaacg gggaggcaga cggccagaaa acccgaaatt ggcatttttt
      961 ctacgccaaa tcggcctcta gccctcgtgg aatctgcgcg agcagctatc aaaacagtag
     1021 cgtttcgggg cgccgcagag ccaccagcaa cccgcgcagc aggctctacc gctcttacac
     1081 caccaccggc tccggctcca gcaggatgcc gaaacgttca tagacgctgg tctggatggc
     1141 cttggccagg gtcatgacct cgccgccggt caccgggtcg gtgctgccgc cgcggttgac
     1201 cagcaccagg gcctggcggt cgtacacgcc ggcgttgcct atgctcttgc ctttccagcc
     1261 gcaggcgtcg atcaaccagc ccgccgccag tttgaccgag ccgtcgtcca ggtggtagtg
     1321 gacgatcttg ggctcgcgct ggatgatgtc ggcgcactgc tcgggcgtca cggtcgggtt
     1381 cttgaagaag ctgccggcat tgccgatcac cttcggatca ggcagtttgg ccttgcggat
     1441 ctcgcagatc cactcataaa tctgtagcgc atcgggctgg gtgatggcgg actgcgccat
     1501 ctttttctcg atgtcggcat aacccagcac gggcttccac aatttgggca acgcaaaccg
     1561 cacgcgtgtg atcaaggcct tgccggccag gcccaggccc gggcgcccgt tcgggccggt
     1621 gctgctgtgt ttgaagaccg aatcgcggta gccaaatgcg cactgcgcgg cgtccagcgt
     1681 gaaacttttt ccggtggtca gatcaatggc gtcgagtgaa tcaaaacggt cctgcaactc
     1741 cacgccgtag gcaccgatgt tctgcaccgg cgagccgccg acggtaccgg ggatcatggc
     1801 gaggttttcc aggcccgggt agccgttgtg cagggtccag cccaccacct cgtgccagtt
     1861 ctcgccggcg ccggcctcga cgatccaggc tttggcggtc tcacccacca gcttcctgcc
     1921 catgatttcc accttgagca cgacggattt gacgtcgccg gtgatgacga tgttgctgcc
     1981 gccgcccagc acgaacttgg gcgccgcctg cagcgccgga tcggccagca cggcggccac
     2041 gtcctcttca ctggcgatac gcaccagcgt gagggccttg gccatgatgc caaaggtatt
     2101 ggaaaactgg aggggaacgt ttttctcgac taacatcacg ggattgtcgc accctgacat
     2161 ccctgcaacg ctaccccgga agccggaaaa ccatgccctc ttttgacacc gttctcgaag
     2221 ccgatctggt caaggtcaaa aactcggttg aaaacaccgc caaggaaatc ggcacgcgtt
     2281 tcgacttcaa gggcacggca gcagccatcg agctcaagga caaggaaatc acgctgatcg
     2341 gcgacagcga cttccagatc gaccagatca ccgacatcct gcgcaacaag gccaccaaaa
     2401 gcggcgtgga cgtgcgcttt ttcgacgccg gcaaggtgga gaaaatcggc ggcgacaagg
     2461 tcaagcaggt catcaaggtg cgcaacggca tcgagaccga acaggccaag aaaatctcgc
     2521 agctgatcaa gggcagcaag gtcaaggtgc aggcgtccat ccagggcgac gcggtccggg
     2581 tgaccggcaa aaaccgcgac gacctgcagg aggcgatggc catgatcaag gcggacctgc
     2641 ccgacatgcc gctgagcttc aacaacttca gggattaaac gtggccccca cgctccccac
     2701 ttcgtgtggt tcgctgcccc ccgagggggc gctgcgcctg cggcccggcg aagccggttc
     2761 cgcggccctg gctggaaggg gatgggggcc tcacgctcgc cgactcgtgc tgtggtttgc
     2821 cgcgctggtg gcctttggcg gcatggcaca cgcgcaaagc gtcgcgctgg ccggcatgct
     2881 gggcagcaag gcgctgttgg tcgtcaatgg tggcgccccg aaatcggtgg cgcccgggga
     2941 atcccaccag ggggtgaagg tggtctccac cggcggcgac caggccgtgc tggagctctc
     3001 gggcaagcgc cacaccgtgc gcgttggcga tgccccggtc agcgtgggag cgggcggtgg
     3061 ctcgggcggc ggcgctggca agggcacacg catcgtgctg accgcgggca ccggcggtca
     3121 tttcttcacc ccgggccaga tcaacggccg ggccgtccag ttcatggtgg ataccggcgc
     3181 gaccggcgtg gccatgagcg ctgccgatgc tgagcgcgcc ggcatcaact acaagggcgg
     3241 ccagccggtg cagatgagca ccgccaacgg cgtgacccag ggctttcgcg tgaaactcaa
     3301 ttccgtgcgg gtcggcgatg tcgaggtgta cgacgtggac gcggtggtgt cgcctcagtc
     3361 catgccctac atgctgctgg gcaacagctt cctgacgcgt ttccagatga tccgcgaaaa
     3421 cgaccagatg acgctggtca agcgatttta gaaacaaaac cggctaaaac ccacaccaca
     3481 caagcgctag cagctatctg attgatagca atcggcggtc tggtcagccc ttcttcttcg
     3541 cacccagctt cgattccgtg cccttgagga gcttgttgat gttttcccgg tgccggaaaa
     3601 tcaggaaggc gctcatggcc acaatggcca gcgcgatcat cttgtcgaca taccagaacg
     3661 agcggtcgcc gatcaggtaa taccagggcg caaaaatggc gcaggcaatg gcggccagtg
     3721 acgaataacg cgaaaagaag gccacgatga cccaggtggc cagcgttgcc aggcccagca
     3781 cccagtgcac gccaaacagc acgccggcag ccgtcgccac ccccttgccg ccctggaaac
     3841 cgaaaaacac cggatagaga tggccgacga aggccgccag gccgacggcg gccagcgtgc
     3901 cttcgcgcat gccgtagtcc ttgccgaacc acatgaccag cgccatcggc agccagccct
     3961 tgaggccgtc gagcagcagc gtgagtgcgg ccgccgcctt gttgccagag cgcagcacgt
     4021 tggtggcacc tgggtttttg ctgccgtagc tgcgcgggtc gttcaggccc atcagccggc
     4081 tgacgatgac cgcgaacgac agcgagccga tcaggtaggc cgccagcacg gccagggcgg
     4141 aataagcgta gtccatggtg ttctctccag tattgttgtt gtagttttgt cttcagtcag
     4201 ccagcgcgca gtggaccgat tttgcaccca gcaggtccac gcacacctgc ggcgcgatgc
     4261 cgacgaggta accgcgccgg ccgccgttga tggcgatttt aggcaaggcc aggatgcttt
     4321 cctcgatata caccggcatc accttgcgcg tgccgaacgg cgaggtgccg cccaccaggt
     4381 agccgctgtg gcggttcgcc acctcgggct tgcagggctc gaccgacttg gcgccaatct
     4441 ggcgcgccag gtttttggtc gacaccttgc ggttgccatg catcagcacc agcagcggtt
     4501 tcgcgtcctg gtcctgcatg accagggtct tgaccacggt gaacggatcg aacccgagca
     4561 cctcggcgct gtgctgcgcg ccgccatgct cgaggtacgc gtaagggtgt tcggtgaaaa
     4621 ccaccccggc ggccttcagc atctgcgttg caggggtggt tttagccccc acggtcgctc
     4681 acttcgtgta gctccctgcc ccccgagggg gccgc
//
LOCUS       DAIDFG010000243         4704 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_243_length_4704_cov_17.365885, whole genome shotgun sequence.
ACCESSION   DAIDFG010000243 DAIDFG010000000
VERSION     DAIDFG010000243.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_243_length_4704_cov_17.365885"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          825 a         1486 c         1620 g          773 t
ORIGIN      
        1 ggaaacgccg gcgctgaaaa tgcgcattga tgtctcggaa aaggacgacg cctaccaggt
       61 caaggccgac atccccggcg tgaagaagga agacatcaac gtgcgcatcg acggcaatgt
      121 ggtgcagatc gacgccgagg tcaagcgcga aaaagagacc aagggcaatg gtgacaaggt
      181 tctgcgcagc gagcgctact ggggcaatat ctcgcgcacc ttcagcctgg cccaggacgt
      241 ggacgacggc aaggtccagg ccaggtacgc cgacggcgtg ttgacgctgg aactgccgaa
      301 gaaggctacg gcagcgacca agaaaatcac agtgcaatag gctcctgcac gcatgaccac
      361 ggcgcagcag ccggacgccc cggtgctgcg catccggtcg ctgtccaaga tctatggcag
      421 tggtgaaacc gcggtacatg ccctgcgcga cgtggatctc gaggtgcggg cgggcgaatt
      481 tctggtgctg ctgggcgcct cgggtagcgg caagtccacg ctgctgaaca ttttgggcgg
      541 actggaccgg ccgagcaccg gtgaggtgga gttcctcgac caccgcctga ccgatgcgcc
      601 cgatgaggag ttgacgcgtt accggcgcga acacgtaggc ttcgtgttcc agttctacaa
      661 cctcattccc agcctgaccg ttcgcgagaa cgtggcactg gtcaccgaca tcgccagcga
      721 ccccatgccg gtggacgaag cgctggcact ggtcgcgctg acgccgcgac aggaccactt
      781 tccttcccaa ctgtccggcg gcgagcagca gcgcgtggcc attgcgcggg ccatcgtcaa
      841 gcgcccgcaa ctgttgctgt gcgacgagcc gaccggcgcg ctggactatg ccaccggcaa
      901 gctggtgctc gaagtgatcg cccgcatcaa ccaggaactg ggcaccacgg ccattgtcat
      961 cacgcacaat gccgccattg ccggcatggc cgatcgcgtc atccgcctgg cggacggacg
     1021 catcgccagc attgaagttc cgtcgcggcg cctcaccccc gcagaattgt catggtgaag
     1081 ccgtgaaagc actggaccgg aagttgctga gggacctgcg gctgctgtgg agccagggcc
     1141 tgaccatcgc actggtggtg gccagcggca taggcggctt catcaccagc ctgagcgcgg
     1201 tggactcgct gggcctggca cgcgaacgtt tttatgccga ggcacatttt gccgatgtgt
     1261 tcgccacggt caaacgcgcc ccgaacgcgt tgatggagac acttcgggag gtgcccggcg
     1321 tggccgacgt gcagaccacc atcgagcagg tggtccgcat agcggctccg ggggtgagtg
     1381 accccatcct cggccagctg atcggcgtgg accgcaaaca gctgccgcgc atgaatcagg
     1441 tgagcgtttc tcgcggccgg gcacttgacc acgcgggact ctcctccggc gcacgcagcg
     1501 acggccgcat cgaggtgctg gtctcggccg gcttcgcgac atcgcggggc gtcaaaccgg
     1561 gcgacatctt caccgcgctg atcaacggga aacaacgcac actcctgatc gccggcatcg
     1621 cgctgtcgcc ggagtatatt tttgccggcc tgggcggcag ccccgaccag cgcggcttcg
     1681 gggtgttctg gatggaccgc gataccctgg ccgcggccta cgacatgcag ggcgccttca
     1741 accgcgtgag tatcaagctt gcccccgggg catcgcagcg cgacgtgatt gcccgcatga
     1801 gccgggtgct ggcacgttac ggcgcgggag aagcctatgg ccgcgaagac cagacatcac
     1861 acgccatgct cgacgccgag atcaaggagc agcaggtgct gggcacggtg ctgccttcca
     1921 tctttctcgg cgtggcagcc ttccttttgc atgtggtggt gtcgcggctg gtatctacac
     1981 agcgggaaca gattgcagcc ctgaaggcgc tgggttacgc caaccgcagc ataggcgcgc
     2041 actatctcaa gctggtgctg gtgatcgtca tcctggggct cttgctgggt atctggctcg
     2101 gcgactggct ggggggcgcc ttcacgcggc tgtatgccga cttcttctac tttccggctt
     2161 tcgagcacca ccttgcgccg tggctgctgg tggtgggcgc gggcctgacg ctggcgaccg
     2221 ccgtggtcgg cacactcagt gccatcggcg ccaccgttcg cctggcaccg gctgaggcca
     2281 tgcgtccgcc cgcgccgggg cactttcggc gcacgctgct ggagcgcctg ggtgtcaagg
     2341 gggtcaaacc ggccctgcag atgatcctgc gcaacatgga gcgccgaccg ctgcgcaccg
     2401 gactgtccat tgccggggtg gccgcagccg tggccattgt ggtgatgggc aatttcatac
     2461 gcgacgcgat cgaccatatt gtggacaccc agttctccct ggccatgcgc agcgacgtga
     2521 tcctgtggat gagcgacccc attggcaatg aggcccgcca tgaggtggcg cgcatgcccg
     2581 gggtgatcgc ggtggaggca acccgcgacg ttccggttcg cttcgtcaac ggcgcagtca
     2641 gcgagcgcgg cctcatccag ggttatgcag aaagccccga gctgcggcgc atcatcgaca
     2701 tccgcggcca ggaagtgcct ctgcccgagg gcggcctggt gatgaccgac cgcctggcca
     2761 gcaagctggg cctgcgcgtg ggtgacaccg tgcgtaccga agtgctcgaa ggacgccgcg
     2821 agacgctgca gctgacactg gcagctaccg cacaggaaat gatggggctg aacgcctaca
     2881 tggagcgccg cgcgctgaac cggctgatgc gcgagggcga cgtggcctcg cagtatgcgg
     2941 tggcgttgca gcgcggcagc gaagccgcct tcctggccgc cagccagggc ctgccgcgcg
     3001 tcgcgggtac tttcagcaaa gccaacctgc tgcgcaacat gcaggaagtc agcgctcgca
     3061 atgtccgcct catgagcctg gttctcaccg tgtttgccag cgtgattgcc gtgggtgtgg
     3121 tgtacaacaa tgcacgtatt gcgctgtcgg agcgaacgtg ggagctggcc agcctgcggg
     3181 tgctgggctt cacacgcgct gaagtctcgg tcttgctgct cggtgaactg gccatcagca
     3241 tcgccattgc actgccactg ggcatgctga tgggctgggg cctggtacac ctgatcgcgg
     3301 aactgctcca atccgaccag ttccagtttc cagtggtgat ccagccgcga acctatgcct
     3361 gggcggccat ctgcgtggtc gccgccggcg tggccagcgc cctggtggtg cgacggcgga
     3421 tcgaccggct cgacatggtg gctgcgctca agaccaggga ataaggaaca ggggaaacag
     3481 ggaatgaatg tgctgaaact gaaaaagaca acctggctat ggatagcggc aggcgtcgcc
     3541 gtgcttgcgg cgctggtctg ggccttttcg ccgcgacccg tcgaagtcga aaccgctgta
     3601 atcaaacagg ggcatttcga gcagtcgatc gaggaggatg gacgcacccg gatcaaggac
     3661 cgctacaccg tctcggcgcc ggtggccgca cgcgtggcac gcatggtgct gcgcgagggt
     3721 gaccctgtgg ccgctggcga cgtcgtggcc gtgctgacgc cggtcatgtc atcgatggtc
     3781 gatgaacgca gcgtgcgcga agcgacagcg cggctgaaag cggccgctgc cggggtggag
     3841 cgcgcctcgg cccgcgtggc gcgcgccaga atttccctgc aggaggctca actggaaaag
     3901 gagcgcaccg agaaactcgc cggtgaagga ttcctgtcgg cctcgaagct cgatgcggtc
     3961 cggctggccc agtcgggcgc ccgccgcgaa ctggaggtgg cggacgcgga gcgtgaaatg
     4021 gcggtgcagg aaaaggccca ggccgcggcg gcgctgcaac cggccgctgc cggcagcagc
     4081 agcacccggc cgctgagcgt gcgttcgccg gtcagcgggg tggtgctgcg cgtgccgcag
     4141 cccagcgagg ccacccttgc cgccggtgcc gccttgctgg acataggcga cccccagcgg
     4201 atggaggtga tcagcgaact gttgaccacc gacgccgtgc aggccacggc cgggcgccgc
     4261 gtggtgatcg agcgctgggg cgggccgccc gtcgagggcg tggtgcgccg cgtggaaccg
     4321 gcggccttca ccaaggtctc tgcgctgggc atcgaagagc agcgcgtcaa tgtactgatc
     4381 gacgtcagca acccgcccga agcatggcgc agcatgggcg acggcttccg ggtatcggtg
     4441 cggatcatta cggccagtgt cgagcaggcc gtgctcgcgc ctctgggggc gctgttcccc
     4501 cacggcaagg gcatggcggt ctaccggctg gacggtcgtg aagcgcggct gcagccggtc
     4561 gaggtcggcg cgcgcaacaa caacgaggcc tggctgcgca gcggcctgac cgaaggccag
     4621 accgtgattg tttacccgcc cgccacactg cgtgacggca gcagggtaca agtacgcaag
     4681 ccctgaccgt gataccgatc gcac
//
LOCUS       DAIDFG010000244         4700 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_244_length_4700_cov_18.243918, whole genome shotgun sequence.
ACCESSION   DAIDFG010000244 DAIDFG010000000
VERSION     DAIDFG010000244.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_244_length_4700_cov_18.243918"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          938 a         1437 c         1490 g          835 t
ORIGIN      
        1 tgctcaacaa gatggatttg ccgcaggccg atccggaaaa cgcgaagcag gaaatcgagg
       61 acgtgatcgg catcgatgcc actgacgcga ttccctgcag cgccaagacc ggcatgggca
      121 tcgacgaaat cctggaggcg gtgattgccc gcatcccgcc gccgcagggc aacccggacg
      181 ggccgctgcg cgcgatgatt gtcgacagct ggtacgacgc ctatgtgggt gtcgtgatgc
      241 tggtgcgtgt ggtggacggc cggctggtca agggcgagcg catcaagctg atggccagcg
      301 aggcgactta caacgccgac cagctggggg ttttcacgcc gcataccgag ccgcgtcccg
      361 cgctggaagc gggcgaggtc ggcttcatca ttgccggcat caaggaactt caggcggcgc
      421 gggtcggcga caccgtgacg ctgatcaggc cgggcaccgg cggcgcggcc gccaccgcca
      481 ccgaggcctt gcccggcttc aaggaaatcc agccgcaggt gtttgccggt ctgtacccga
      541 ccgaagccag cgagtacgac tcgctgcgcg acgcgctgga aaagctcaag ctcaacgatg
      601 cgtccctgca ttacgaaccg gaagtcagcc aggcgctggg ttttggcttt cgctgcggtt
      661 tcctgggcct gttgcacatg gagatcgtgc aggagcggtt ggaacgcgag ttcgaccagg
      721 acctgatcac gaccgcgccc agcgtggtct accaggtgct caaggccgac ggcgagatca
      781 tcatggtcga aaacccgtcg aaaattcccg atgccggcag ggtcaacgaa atccgcgagc
      841 ccatcgtgac cgtgcacctg tacatgccgc aggagtacgt gggcgccgtc atgacgctgg
      901 ccaacctcaa acgcggcgtg cagctcaaca tggcttacca cggcaagcag gtcatgctga
      961 cctacgaaat gccgctgggc gagatcgtgc tggacttctt cgacaagctc aaatcggtct
     1021 cgcgcggcta cgcgtccatg gattacgagt tcaaggagtt ccgggcgtcc gacgtggtca
     1081 aggtcgacat cctgctcaac ggcgagaagg tcgatgcgct gtccatcatt gttcaccgca
     1141 gccagtcggc ctaccgcggc cgtgcggtgg tggccaagat gcgcgagatc atttcccgcc
     1201 agcagttcga cgtcgcgatc caggccgcca tcggggccaa catcattgcg cgtgagacaa
     1261 tcaaggcgct gcgcaagaac gtgattgcca agtgttacgg tggcgacatt tcacgcaaga
     1321 aaaagcttct tgaaaaacaa aaagcgggaa aaaaacgcat gaaacaaatc ggttcggtgg
     1381 aagtccccca ggaggccttc ctcgccatcc tgcaggtaga agaataatgc tgggtttcat
     1441 gagtacgctc accgcgctgg tgctgatcct gtttgccggt tacggcggcg cctggtacat
     1501 gggcatggtg gaaggcaact tctccctgct gctgtttctg gcgaccgtgg tgacgggcct
     1561 ttactggctg gccgagaagt tttacttctg gccccagcgc cgcaaggcgg ccgaaatcat
     1621 cgagcaggag gcggtccggc gccgcagcga actggccgcc cgcggcatca gccaggtcga
     1681 tggcaacatc gaggaggccc gggcgagggt tatcatgcag ccttggtggc tggactggac
     1741 cgccgggctt ttcccggtca tcgtggtcgt gtttctgttg cgttctttcc tgttcgagcc
     1801 tttcaagatc ccgtcgggct ccatgattcc gaccatgctg gtgggcgacc tgatcctcgt
     1861 caacaaattc cattacggcg tgcggcttcc ggtcatcaac ctcaaaatcg ttgacaacaa
     1921 cagcccgcag cgcggcgatg tgatggtgtt ccgttacccg ccccagccca gcctggacta
     1981 catcaagcgg gtggtcggtg tgcccggtga cgaggtggcc tacctgaaca aaaaactcac
     2041 catcaacggc aagcctgtat ccaaggtggc gctgcctgat ttttttgacg aagacgccct
     2101 gcgttacgcc aagcagttcc aggaaaccag cggcaacaag ccctaccgcg tgctcaatga
     2161 cgaggaccgg ccggccttta ttgccggcgc agccgacttt ccctatcggc aaaattgccg
     2221 ttacagtagc gagggcgtgg tttgcaaggt gccgcagggg cactatttca tgatgggcga
     2281 caaccgcgac aattcgctgg attcgcgtta ctggggtttt gtgcccgagg ccaacatcgt
     2341 cggcaaggcc ttttttgtct ggatgaattt tggtaacctc aaacgcatcg gcaaggtgga
     2401 ctgagggatg atccgggctg gccctgccgg caaggcaggg gcggcgcggg gcatggggcg
     2461 caactaacaa cagctgggga aacacatgat gaaaaatcgg caacgtggca tttcgttcat
     2521 cggcatcctg ttcgtgggcg gtgtactggc tttcgcgggc gtgatcgcgg cccaggtgtt
     2581 tcccacgctg gtcgagttcc agtccatcac caaggcggcc aacaaggcgg ccgagggcag
     2641 caccgtgccc gaggtgcgga tgattttcga caaggcggcc gcgattgacg acatcaggtc
     2701 gatcggcggc aaggacctcg aagtcggcaa agagggcgac aaggtcattg tcagttttgc
     2761 ctacaacaag gaaatccaca ttggcgggcc ggcttacctg ctgctcaagt actccggccg
     2821 ctccaaataa gtgcgtgcca gtccttcttt gagcccggac ctgcaggcgc tgcaaacgcg
     2881 tctggagcac gaattttcca atccgcgcct gctgcagcag gccctgaccc atcgcagttt
     2941 ttcggccgac cacaacgaac gtgtcgagtt tttgggtgac tccgtgctca acctggcggt
     3001 ggccgggctg ctctacgagc ggctgagcga gttacccgag ggcgatttgt cgcgggtgcg
     3061 cgccaacctg gtcaagcagg acacgctgca caagctggcc gtgcagctcg gcctgccgct
     3121 gttgatacgc ctcggcgagg gtgaagccaa gtcgggtgga caaaaacggc cgtccatcct
     3181 ggccgatgcg ctggaggcgg tcattggcgc cgtgtttctg gacgccggtt tcgacaaggc
     3241 cgaccgcctg gtgcgccggc tgttccagga cgtcgaaatc aacccgacca tgcaggccat
     3301 cggcaaagac ccgaagacgg agttgcagga atggctgcag ggccgcaaaa tgaacctgcc
     3361 gatctaccgc gtggtcggaa cgctgggcgc agcccacaag cagacctttg acgtggaatg
     3421 tgaaatcagc gaattcggcc gtgcggagcg cggcattggc ggctcgcggc gcgccggcga
     3481 gcaggcagcg gccgccgcga tgctggctta tgtaaaaacg ctcccttgat ttgaaacagg
     3541 cgctgcaagc gcagccgcca acaggcgacc cccatgaccc ccgcaaaaaa gactccctcc
     3601 aagccggcac gcccggcagc gcctgccggc actaccaaag ccaaggccaa ggctgcgcct
     3661 gctgttgaac cggcagaggc cgttgtgccc gcgcaggacg cggccctgga agccatgctg
     3721 gccaaggcca tgacctcgca ggccaacaac gccccgccgg tcgaaggcca gcgctgcggc
     3781 ctgatcgcca tcgtgggcaa gcccaatgtg ggcaagtcga ccctgctgaa cgcgctggtg
     3841 gggcagaaaa tcagcatcac ctcgcgcaag gcgcagacca cgcggcaccg catcaccggc
     3901 atgcgcacgc tgggcgccac ccagtttgtg tttgtcgata cccccggttt ccagacccgg
     3961 cacggcaacg ccctgaaccg ctcgctcaac aagacggtgg tcggcgccgt caacgacgtg
     4021 gacctgattg tgtttgtcgt cgaggcgggc cagttcaacc aggccgatgc caaggtgctg
     4081 tcgctgttgc cggcgaacat gccggccatc ctgctggcca acaagttcga cctgatccac
     4141 cgccgcgcgg atatcgcgcc ctggctcaag gagatgcagg cgcggcacaa ttttgccgag
     4201 ttcgtgccga tgtcggccaa caacgccaag gatgtcgagc gcctgttcgg catctgcgag
     4261 aaatacctgc ccccgcagcc ctggatgtac ggggccgagg aactgaccga ccgcagcgag
     4321 cgtttcatgg cggcggagat catccgcgaa aagctgttcc gcctgaccgg cgacgagctg
     4381 ccctacacct cgaccgtgat catcgacaag ttcgagcagg agggtgagtt gcgccggatc
     4441 gccgccacca tcgtcgtcga gcgtgacggc cacaagggca tgatcattgg cgagaagggt
     4501 gaaaagctca agcgcatcgg caccgaagcc cgccatgaac tcgaagccct gaccggcggc
     4561 aaggtgttcc tggaaatctg ggtcaaggtg cgttccggct gggccgacga cgaggctcgc
     4621 gtcaagacct tcggctatga gtgattggcc cccacggtcg ctcactgcgt gtagctccct
     4681 gccccccgag ggggccgctg
//
LOCUS       DAIDFG010000245         4692 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_245_length_4692_cov_19.583783, whole genome shotgun sequence.
ACCESSION   DAIDFG010000245 DAIDFG010000000
VERSION     DAIDFG010000245.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_245_length_4692_cov_19.583783"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          782 a         1545 c         1544 g          821 t
ORIGIN      
        1 agcagcacgc tggcgcccac gagcccgagc acgacggccc agggccaggc cgcttcgccc
       61 gtgtgcagca ccacggcgag gtcgtaaacg cgctgcgcaa aggtggcgtc ctgccaggcc
      121 agcatctggc ccgagtacct gtcgatccaa ccttgaccct ggacggtggc gaccttccag
      181 gtgtcctccg ggtcggtgat accgggaaaa ttcagcttgt gcagatcccg cacaacaagg
      241 ccttgcagcg tggcaagctg tgcgccaggc aaggctggct tgccggcgac caccgagagc
      301 acttcaggct cggttctggt gtccagtgcc acgaggccca gggtcgatgc gctcatggtc
      361 agcgccgtga gcgatgtcag gaacaggact gcgagcacca cacggcccgc gaccacatgg
      421 atgcgctgcg ccagcgagcc acgcacccgc gccgccagcc gccgccagcc gcccatgcgc
      481 cgcagcagca gcaccagcgc agaaacgcac accaggccca tggccagcgc aatgcccgct
      541 gcgccccagc gcccggcgtc gccgagcagc agcgagcggt gcaggttctt tacccagcgc
      601 ggcagcgccg aggcttgcca cgcacccagc acccggccat cggcggggtc cacgtaagac
      661 gcctgcggct ggtcgccggc aaaactgaac accacgatgg cacccgaggg caggtggcga
      721 atttcttccg cgcccgggac ggtgcgcgtc acgcgctcca ccagcgtggc cacgggcaaa
      781 tcgcccggtg cggcgggtgc ctgccacgcc tgctgcaccg gatcaatcgc caggatggct
      841 ccggtgacgc ccagcaccac ggccagggtg ccaacggtca ggcccagcca gcggtggatg
      901 gctttccagc ccatacgagc cctttactgg agttggaagg tcaccgactg gatgtactgc
      961 ttgccggcct gcggcttgcc ggcgttggct ttggacagcg gaacgcggac ctctgacggg
     1021 ctgtcgcgca tgtcttctgc tgcggcgtcg atgcggattt catagcccgc gtcaatcagt
     1081 gcatccgcca gatcggctgt gaccttgagt gtgcgcccag cgcccacgct ggcgccggtg
     1141 acgccgtcca gacgcttggc gtcaccggcc gagaggcggc tccagtcgga caagtgcttg
     1201 tggtacttcg ccttgccgcc ggccacccac agcgtgcgca cataggcgcc tttggcatcg
     1261 gtcaggtacg ccgccaggta ggcgccgtcg ccaccgtagt tcttgagctg ggcggtcagc
     1321 gtgacctggc ggctgtgtgc cagcgcggga agcgccagcg cgcaggcggt ggccaaaatg
     1381 gtcaaaagag gtttggacat ggtgatgctc ctgtgggatt ggtgcttact ggatctggcc
     1441 gcgcggcgcg ctgccagggg tcaacaaagg cgatggcgcg gcagcgccgt ccggttgcgg
     1501 cggtcgtgcc tgctgcgcgg caccctcctg accacgatcg cgttcccggg aacgttggtg
     1561 gtcgtcttgc ttcatcttca acaccttcag ggtttccggg tcgatcttgg ccttgaaggt
     1621 gcgtccctgc gcatccgtgc cgcgaatttc atagcagccg tcgtcgatct tcagccgatg
     1681 gatctgccag ccctgtgcgg ctgccatctg ccgcacagcc tcacgcgtct gccagcgatt
     1741 gagcggcgca tcacaatcgt caccggccca tgcgggcagg gtgaccaggc ccaccgacaa
     1801 aaccagcaag ccatggcgca aagaggcggg gtatttcatg ggagggtcct ttaaaacggg
     1861 gcagtacatg atgaacggtt tgcggtgccg aaaatgccag ggctggtgcc cggcagccaa
     1921 attccagctg caacgttgat acgcatcagg gttctgcctt cttgttcaag gggattgtgg
     1981 agaaaacacc ctgtatgcat gctgaatggc gcattcattt tctgttcatc gcccaaaagg
     2041 caaaacgcgg gctggtggtc ttgtgcagct gcgctgcccc gtcgctgcct tgttctgtcc
     2101 ggcctgggaa tgtagtgccc cgatgggttc ggccagccag ggcttgttca caccatgaat
     2161 acaggtgcga acgcggtgaa cacagcgcca atcaaggcgt gcgacgacgc ccggaccggg
     2221 cgtccgggcg aggagtgcaa cgacaagtgg cgttggagaa accggctatg ctgatactgt
     2281 ccgactcagc gaacccagtc tgttcgtctc cttgggctgc agcccacttt ggaatttgag
     2341 tccgagcagg ccgcgacgag tcaggctgcc agttctttca tcaccgcata caggtccgcc
     2401 ttgccctcga agccgatgcc cggcaattca ggcagcgtga cgtagctgtc caccaccttc
     2461 acaccatcgg gaaaaccgcc atagggctgg aacaggtccg ggtaggactc gttgccgccc
     2521 aggcccaggc cggccgcaat cgccagggac atctggtggc cgccgtgcgg aatgcagcgt
     2581 gtgggcgacc agccatgctc cttgagcatg tccagcgtgc gcaggtattc gaccaggccg
     2641 tagctgagcg cgcagtcgaa ctgcagccag tcgcggtccg ggcgcatgcc gccgtaacgg
     2701 atcaggttgc gcgcgtcctg catggaaaac aggttctcgc cggtggccat gggccccgcg
     2761 tagtgctgcg ccagctcggc ctgcaaggcg tagtccagcg ggtcgccggc ctcctcgtac
     2821 cagaacaggt cgtagtgaga cagggccttg gcgtaggcga tggcagtctt caggtcgaag
     2881 cggccattcg catcgaccgc cagcttctga ccaggggcca gcatgcccag caccgcctcg
     2941 atgcgccggc agtcgtcggc cagcgacgcg ccgcctattt tcatcttcac caccgcgtaa
     3001 ccgcggtcca ggtagctgcg catctccatc atcagcttgg cgtcgtcctt gcccgggtag
     3061 taataaccgc cggccgcgta cacaaacacc ttgcggttcg gcgtgccgtt gccataccgt
     3121 tcggccagca gctggtacag cggcacgcct tcaatcttgg ccacggcgtc ccagacggcc
     3181 atgtcgatgg tgccgacggc caccgaacgt tcgccgtggc cgccgggttt ttcattggtc
     3241 atcatggcgg cccaaacctt gtgcgggtcg aggttgtccc ggctgtcgtc cagcaggctg
     3301 tccggcgcgg cttcgaggat gcgcggcgca aaacgctcgc ggatcagccc gccctggccg
     3361 taacggccgt tggagttgaa gccgtaaccg acgacaggtt tgccgtcgcg gaccacgtcg
     3421 gtgacgacgg ccaccaggct cagtgtcatt ttcgaaaagt cgatgtaggc gttgcgaatg
     3481 tcggacttga tcggctgggt gacttcgcgg atggctgtaa tcttcatgtc tttgctttgc
     3541 tggaggtggg tgatgggact atcatcggcg cctcgcgtcc cggcgtccaa tgccggtttt
     3601 ttcttgttcc atgcccgcaa agcaccgccc atgaacctga cctggttgga ggatttcctg
     3661 gcgctcgcca gctcgggcaa tttctcgcgc gccgccagcg accgccacat gacacagccg
     3721 gccttcagcc gccgcgtgcg ggcgctggag gagtggctgg gcgtcgctct gttcgaccgc
     3781 agcacgcacc ccgtcacgat gaccgagacc gggcagtggt tccagtcggt ggcgcaggac
     3841 atcctcgcgc gtgtggcccg tgtgcccgac gaggcgcggg cagtggccga cgccagctcc
     3901 gccaccctgc ggtttgcgtc cacccacgcg ttgtccttca ctttcctgcc gacctggctg
     3961 cgcgggctgg aggccggcgc gactatgggc ccgatccagc tcgtctcgga cgtgatgcag
     4021 cagtgcgagg cgctgatgct gcaggggcgc atccagttcc tgctgtgtca ctggcatgaa
     4081 caggtgccgg gccggctgga cccggcgagc caccgctcgg tgaagctggg cggcgacacc
     4141 ctggtgccgg tctcgagccc ggggaaaaac ggcaagcccc agcacctgct gtcggccagc
     4201 ggcaaacgag cgcccatcct cgcctacagc agcgagtccg ggctgggccg catcgtgcgc
     4261 gccctgcgca gcggcgcgct tgaaaaatcc cgcagcgagc cggtgttcac cgcgcatctg
     4321 gcctcggtgc tcaagaccat ggcgctggac ggacgcggcg tggcctggtt gccgctcagc
     4381 ctgatcgccg gggaactggc cacggggcgg ctggtcgccg ccggcggccc cgagtgggcg
     4441 attgaactcg acatccggct gttccgcagc aataccgcgc tgccgccggc ggccgaaaac
     4501 ttctggcgcg gggtggccgg ttcgccgcgc taggagtctg cgcggcagaa ggcgtcgaag
     4561 cgaaaagcgg ccccagcgag gacagttttt gccggatttg cagccccata gccgtcgcta
     4621 tggggcaata agccggcgaa aaatggaccg ctgcggtcgc tttgcagccg acgatccttc
     4681 tgccgcgcag ac
//
LOCUS       DAIDFG010000246         4686 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_246_length_4686_cov_17.341827, whole genome shotgun sequence.
ACCESSION   DAIDFG010000246 DAIDFG010000000
VERSION     DAIDFG010000246.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_246_length_4686_cov_17.341827"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          826 a         1603 c         1490 g          767 t
ORIGIN      
        1 gtaagcaaag agaaggcgac ccgatggtct gggtccctcc gcttcgctgc gggcaacctg
       61 cggtgctcgc cgaaagtggg gccgagctcg aactcgcctt cggctcagac aatcgctcgt
      121 cctgatccac tttcggctgc gctcctcggc ccagcccgac gggtggggaa caaccaatgc
      181 gggaaaaaga atccggagtc cttgaagcgc gcagcgcttc aaggacggga atcccaacag
      241 cccgtcatgt ttgcaagctt ttcaagcctt caaaaactcc gccttccccc ccagccaccg
      301 caagatatgc cggtgcgcca gctcagggta cttgtccagc atcaccggcg ccagggcccg
      361 cgcccaggcc agcagctcgg tatccgtagc caggtccgca aaccgcagca gcggcgcgcc
      421 cgactggcgt gcccccagaa attccccagg gcctcggatc tccagatcgc gccgggcgat
      481 ttcgaagccg tcctgggttt ccaccatggc cttgagccgg gcgcgtgcgg tctcgcccag
      541 gcgcggcgct tcgccggtcg aataaagcaa cacacaggcc gaggcggcgg cgccgcgccc
      601 gacgcggccg cgcagctggt gcagctggct caggccaaaa cgttcggcgt gttcgatcac
      661 catcagcgag gcgttcggca cgtccacccc cacctcgatc accgtggtgc tgaccagcac
      721 gccgagctgg ccactggtga acaaggccat caccgctttc ttttcagccg gcggcatgcg
      781 tgagtgcagc aggccgacgg gcgtaccggg caaggcggcg ctcagcgctt catgggtctg
      841 ggtggcgttg atcaggtcga ccgattcact ctcttcgatc agcgggcaga cccagtagac
      901 ctgccggccc tcggcgagct ggctgcggat gcgctccacc acctcgtcgc gccgcgcctc
      961 gctgaccacc ttggtgacga tgggtgtgcg gccgggcggc agctcgtcaa tcgtcgaaac
     1021 atcgaggtcg gcgtaataac tcatggccag cgtgcgcgga atcggcgtgg ccgtcatcat
     1081 caagaggtga ggttcctcgc caccctcggt catcttgctg cgcagggcca gccgctgcgc
     1141 cacaccgaag cggtgctgct cgtcgatgat ggccagcgcg aggttcttga aaaccacctt
     1201 gtcctggatc accgcatggg tgccgaccac caggccggcc gcgccgcttt cgatcatggc
     1261 cagggcttcg cgccgctcct tcgccttctg gctgccggtg agccaggcgg tctggatgcc
     1321 cagcggttcg agccagccga tcagcttgcg aaagtgctgc tcggccagga tttcggtggg
     1381 cgccatcagc gcacactgcc agccagcgtc catgcagcgc gccgcagcca gcgccgccac
     1441 cacggttttg ccggagccca cgtcaccctg caacaagcgg tgcatgggaa cattcctcgt
     1501 cagatcggcc gcgatctcgg tgccgacgcg ctgctgcgcg gccgtcaggc gaaagggcag
     1561 gcgctccagc agttgctcct gcagcgtgca ctgggggccg tgtatgggcg accgcaacgg
     1621 cggcgcccgc atggcggcgc gcacgcgacg cgcctgcagc tgcgacaact gctgggccag
     1681 cagctcctcg gccttgagcc gctgccaggc cggatggctg cggtcctcca gcgtggccag
     1741 catcacgtcc ggcgtcgggt ggtgcaaaaa ctgtagcgcc tcgcgcaggc tggacaaggg
     1801 ctggggcagg tttttacttg taaattcggc cggaagggtt tccgccagat cggcccgggc
     1861 cagcccgcca agcacggcct tgcgcagata ggcctgcggc aaaccggcca ccgtgggata
     1921 caccggcgtc agggcggtcg cgagttcacc gccggcggcg cgaaagctcg ggtgcaccat
     1981 ttcgcggctg acaaaacccc cgcgtatctc cccgcgcacc cgcacgcgcg cgcccacact
     2041 gagggttttc tggtgcaccg ggtaaaagtt gatgaaacgc agcacgcagg tgtcgctgcc
     2101 gtcgtccacc gtcacacgca gctgtcggcg cgggcggaac gtgatttcgc tgtgggtgac
     2161 ctgcgcctcg atctgcacca tgtcaccgtc gcgcgcatcg gccagccggg tgatgcgggt
     2221 ttcgtcctcg taacgcagcg gcaggtgcag cgccaggtcg atgtcacggc gcaggcccag
     2281 cttttccagc gctttctgcg gctgggactt gggcgccacc gcgttgccgg tgccagcctc
     2341 agccggcggc gccttgcggg gagaagctgc ggggggggac atgggacaat tgtgcatcca
     2401 tgaagacctt gttccgcctc agtgactttg atttcacgct ccccgacgaa ctgatcgccc
     2461 agcacccggc cgcagaacgc agcgcctcgc gcctgctgga cggccggggc gacacccctg
     2521 tggaccgccg ttttaccgag ctgccggacc tgctgcagcc cggcgacctg ctggtgctca
     2581 acgacaccca ggtcgtcaag gcgcggctgt tcgggcagaa aagcagcggc ggccggctgg
     2641 agctgctgat cgagcgggtg ctctcaccca cgccggatgc cggccatgaa gtcgccgccc
     2701 acatgaaggt cagcaagaag ccgctgccgg gcgcggtgct gcagatggac ggtggcttca
     2761 ccgcaacgct gctgggccgc tggcccaacg aggacggacc gctgtaccgc ttcgccctga
     2821 gcagcgatcc gtatgcgctg atggccagcc atggccatgt gccactgcca ccctacatca
     2881 cccacaccga ctcggccgtg gacgagcaac gctaccagac ggtgtttgcc aaaaacccgg
     2941 gcgcggtcgc cgcaccgacc gcagcgctgc attttgatga cgcggtgctg gcgcggctgg
     3001 aagcgcgtgg catccagcgc gccaccgtca ccctgcacgt cggtgcaggc accttccagc
     3061 ccgtcaagac cgagaacatc gccgatcacc agatgcattt cgagcgcttc gaggtgccgg
     3121 aatcaacact gcaggccatc acggcgtgca aggcgcgcgg cggccgcgtg gtcgcggtcg
     3181 gcaccaccag cgtgcgggcg ctcgaatctg ccgcgaagtt cggccccgct tgccgcgaca
     3241 ccaacatctt catcaccccg ggcttcgagt tccaggtggt ggacctgctg ctgactaatt
     3301 tccacctgcc caaaagcacg ctgatgatgg tggtcagcgc ctttgccggc tacgaacaca
     3361 tcatggcgct gtaccgccac gcgatcgccc agcgctatcg ctttttcagc tacggcgacg
     3421 ccatgctgct gcaacgacgc ccctgagaca atcaccccat gctgaaattt gaagtcctga
     3481 ccaccgacgc gcccaccacc ggccccgagg gcgcctacct cggctccttc gcccgccgcg
     3541 gcacgctcac cctgaaccac ggcgtggtgc agacccccat cttcatgccg gtcggcacct
     3601 atggcacggt caagggcgtg atgccgcagt cgctgcacga gatgggcgct cagatcatcc
     3661 tgggcaacac cttccacctg tggatgcgac cggggctgga cgtgatggcc caattcggcg
     3721 gcctgcacaa gttcgagaac tggcacaagc ccatcctgac cgacagcggc ggcttccagg
     3781 tctggtcgct cggcgagatg cgcaagatct ccgaggaagg cgtgaagttc gcctcgccag
     3841 tcaacggcga caagctgttc ctgacgcccg agatctcgat gcagatccag accatcctga
     3901 acagcgacat cgtcatgcag ttcgacgaat gcacgcctta cgacaccaag ggccacatca
     3961 cgaccgagaa cgaagcgcgt ttttcgatgg agctgagccg gcgctgggcg aaacgctgcg
     4021 aggacgagtt tgcccggctg gagaatccca acgcgctgtt cggcatcgtg cagggcggca
     4081 tgttcgaaaa cctgcgccag gaatcgctcg acgccctggt cgagatggac tttcccggct
     4141 acgccgtcgg cggcgtcagt gtcggcgagc ccaaggaaga gatgcagcgc atcatggcgc
     4201 acacgccgca ccgcctgccc gcccacaagc cgcgttacct catgggtgtg ggcacgccgg
     4261 aagacctggt cgaaggcgtg gccgcgggcg tggacatgtt cgactgcgtg atgcccactc
     4321 gcaatgcgcg caacggccac atgttcacgc gcttcggtga cctgaaaatc cgcaacgcgc
     4381 gctacaagac cgaagaggcg ccggtggaca gcacctgcag ctgctacacc tgcaaaaatt
     4441 tcagccgtgc ctacatgcac cacctggacc gctgcggcga aatgctgggg ccgatgctgt
     4501 cgagcattaa caacctgcac tactacctga acctgatgca ggaagtacgt gacgcgctgg
     4561 acgccgggcg ctttggcgcc tgggtgacgc agttccatgc cgaccggtcg cgcggggttt
     4621 aaggcatgaa catcgatgcg caacgctggc tggatcgctg ggtcggcata ccgctgtgtg
     4681 cggccg
//
LOCUS       DAIDFG010000247         4662 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_247_length_4662_cov_22.543087, whole genome shotgun sequence.
ACCESSION   DAIDFG010000247 DAIDFG010000000
VERSION     DAIDFG010000247.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_247_length_4662_cov_22.543087"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          968 a         1492 c         1476 g          726 t
ORIGIN      
        1 ctgggcggct cactgggccg gcgcgaagcc accggccgcg gcgtgttcac cgtcggcgcc
       61 gaagcggcca gacacatcga cctggacatt gcgaccgccc gcgtggcggt gcagggtttt
      121 ggcaatgtgg gcggcgtggc cggcaagctg tttgccgaag ccggtgcacg cgtcgtggcc
      181 gtgcaggacc atggcggcac gatttaccgt gaagccgggc tcgatgtgcc ctcgctgctc
      241 agacacgtgg cggagaccgg cagcgtggcc ggttttgcgc aggccgaagt catcgccgac
      301 gacaagttct gggacgtgga ttgcgagatc ctgatcccgg ccgcattgga acagcagatc
      361 accagggaca acgccgggcg catcaaggca cgcatgatca tcgaaggcgc caacgggcca
      421 accacccccg aagccgacga catcctgcag gaacgcaatg tgctggtact gcccgacgtg
      481 attgccaacg ccggcggcgt gaccgtcagt tacttcgagt gggtgcagga tttctccagc
      541 ttcttctggg acgaagccga aatcaacgcg cgcctggtgc gcatcatgaa ggaagccttt
      601 gccggcgtct ggcaggtggc gcaggaccac aaggtcagcc tgcgcactgc aacgtttatc
      661 gtggcctgca aacgtattct gcatacacga gagctgcgcg gtttgtaccc ctgaagcatg
      721 tgacccccac ggtcgctcac tacgtgtagc tccctgcccc ccccgagggg ggccgctgcg
      781 cctgcggtct ggcaaagcca gtcccgcggc ccttgcttga ttgcgaccgg gagctcgtgg
      841 ctgcttactg aagcgtttcc ttgacggcct cggccagcgg cagcggcatc acgtcgatgc
      901 cctcttccag caactcgacc gcttccgccc ggctggcctg gccgcggatg ctgcgctgtt
      961 cggcgtcgcc gtaatgcatg gcgcgtgcct cgtcggcgaa ccgttccccc acatcctcgg
     1021 tgttggccat cacgtggcgc aaggccttca ggaactcggc ctgcacggcc gggctaccgg
     1081 cgccagccat cacctcatgc accgggttgc cggccggtgc agccgcgggc acggacagtt
     1141 cacgcgaggg tgaagccact gccggcgccc cgctcgcggc gcccagattg agccgggggg
     1201 cgctgggcat tttccgcacg gccgggtcgg cgcacagcgg gcaggtcacc aggccgcgcg
     1261 caagctggcc ctgaaaatca tcttccgacg caaaccagcc ttcaaaggcg tgctggtgcg
     1321 aacactggag gttgaggact ttcatgggct gattatcagc ccgcggtgcg ggaagtgccg
     1381 gtctgccagt ccagcgtcca tgcgcccgac aggacgttct gcagccacag cgcgcccgtc
     1441 agtgtcttgc cgtcggtgac acgtccatcg cggcaccact gcagcagttc ggccggcgtg
     1501 gccgtgaaca cgtcgagaaa ttcgcccgcg tcgagcttgc gccctccggg ggtcaggtcc
     1561 ctggcaaacc agatgtcgat gaattcggtc gagtaggaaa tcaccgggtg catgacaccg
     1621 gcgcgggccc aggaagccgc gctgtaaccg gtttcctcga gcaactcgcg ctgggcacag
     1681 gccagcggct cctcaccggc atcaagcttg ccggcgggaa actccagcat cacggcctgc
     1741 accggatagc ggtattgccg ctccagcacc aggcggccgt cgccgagctg ggcaatgatc
     1801 atgaccgcac ccggatggcg cacatactca cgcgaggctt cccgtccgtc cggcaggcgg
     1861 accgtgtcgc ggaaagcgtg gaggaaatgg ccgtgcaaca gctcctggct gccgaccttg
     1921 tgctcgatca gatggctgtc ggccgtacca aggggcgctg cgggatgctc aggcatgtcg
     1981 gaagaccggg cgggcgccgc cgctcaacga tgtttaaaca ggtaacgaaa cacaaagccc
     2041 ggaaaggcga aggtgatgaa cagcgtggcc gtgatcgcgt agaactccca gccctgcggc
     2101 gcgatctggc cggcgcgctg ctccagcagc agcgcgatac cgcctgccac gaaataccag
     2161 aacaccatct cggcaagacg caccgccaga tttttggggt tctgcaaccg gtaaaccagc
     2221 agtacgcggt tgctgatgaa aggcaggttg gccgccagca gggccagcgc caccaccaac
     2281 catatcgaga cagtctggtt catgcgccga gggacttcac gacgctggca atcgcctgct
     2341 ggcatagcgt catcaggccg ccgggcatga cacccagcag cagcagcagc gcgccgttga
     2401 tggacagcac cacgcgggca tcggccgggg ccacgatggc cgtgggcatg ccgacgttgg
     2461 gggcatcaaa atacatcacc ttgacgaccc gcaggtaata gaaagcaccg atcagcgaca
     2521 tcaacaccgc aaaaaccgcc agcacgatgt agctggtctg ttgcgaggac accagggcct
     2581 gcagcacgcc gagcttggcg tagaaaccaa ccacaggcgg cacaccagcc agcgagaaca
     2641 tgctgatggc catgacgccg gcaaacagcg ggctacgctg attcaggccg gccaggtcgg
     2701 tgatttcctc ggactcgtgg ccggcacgtg ccagcagcag gatgatgccg aacaccgcga
     2761 gtgtggtcag cacataggtg atggcgtaaa acatggccgc gccataggcg tactcggcat
     2821 tgaggggcct gccgggaacc acagcagcca gcagcgccag cagcaggaag cccatttgcg
     2881 aaatggtgga gaacgccagc atgcgcttca gattggtctg cgccaccgcc gccagattgc
     2941 ccaccagcag cgaaccgatg gccatcagcg ccagcatctg ctgccagtcg atagccagcg
     3001 gcagcaggcc ctgcaccagc aagcggatgc agatggcaaa ggcggcaaac tgcggcgccg
     3061 caccgatcag cagcgtgatg gccgtgggcg caccctggta aacgtcgggc agccacatgt
     3121 gaaacggcac ggcgcccagc ttgaaggcca ggccggccac aatgaacacc agaccgaaca
     3181 ccagcacctg gtggttcacc tggcggctgg caatggccct gtacacctcg ttgatgtcca
     3241 gagagccggt ggcgccgtac atcatggaca ggccgtaaag caggaagccc gaggccatgg
     3301 cactgagcac aaagtatttc atcgcggctt cggtagccgt tgcgttgtca cggcgcagcg
     3361 ccaccagggc gtagctggac agcgtcatca gctccaggcc catgtagagc accaggaagt
     3421 tgttgccgga gatcatcacg aagatgccga gcagcgcgaa caggcccagc gtgaacagct
     3481 cgccgccgcg cagcatgtcg cggtctgccg catacccacg gccatacacc agggtgacca
     3541 tcatcgcgat cgaggcgaaa cacttgagcc agttgcccat ggggtcgctc acgaccatgt
     3601 tgccgaagcc gtagaacgtg ttgcccttga cggcgtacac agcctccagt gccgccacgg
     3661 cagccagcgt cagcagggtc agcacgtagg tcagggtgcg ctgcgggctt ttcacgccga
     3721 ggtcgaccag ggcgatcacg caggccatga ccagaagcac gatttccggg tagaccgata
     3781 gccaactgag tttgtcaatc atctgaattc tttgaaatgg ttgcttattg cagtttggaa
     3841 gccgcgacgt gtttgagcag gtcgatgacc gagctgtcca tcacatcggt gaaaggcttg
     3901 ggatagatac ccatgaacaa cacggcaatg gccagcaggg ccagcatcag gaattcacgg
     3961 ctgttgatgt cggccaggcc cttgacatcg tcgttggcga ccggtccgag gtaaacacgc
     4021 ttgaacatcc acagggtata ggctgcgccg aaaatcagtg ccgacgcggc cgccgcgcca
     4081 atccagaagt tgaatttgac tgcgcccagg atgaccatcc actcgccgac aaatccggcg
     4141 gtggcgggca ggccgcagtt ggccatggca aacagcagcg caaaggccgt gaacttgggc
     4201 atggtgttga ccacaccacc gtaactggcg atctcgcgcg aatgcacccg gtcgtacaac
     4261 acgccaatac acaggaacat ggcggcggaa acaaagccgt gcgcaatcat ctgcacaatg
     4321 ccgccggaca caccgatgtc gttgaagatg aaaaagccca aggtgacaaa acccatgtgt
     4381 gccaccgacg aatacgccac cagcttcttc atgtcccgct ggaccatggc caccaggccg
     4441 acgtagatca cggcgatcaa cgacagggca atcatgagtc cagcccattc acgtgcggcg
     4501 tcgggcgcaa tcggcagcga aaaccgcagg aaaccgtagg cgccgagctt gagcatgatg
     4561 gccgccagca cggccgagcc gccggtgggc gcctccacgt gcacgtcggg cagccaggtg
     4621 tggaccggcc acatcggcac cttcacggca aacgccgcca gg
//
LOCUS       DAIDFG010000248         4656 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_248_length_4656_cov_20.876331, whole genome shotgun sequence.
ACCESSION   DAIDFG010000248 DAIDFG010000000
VERSION     DAIDFG010000248.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_248_length_4656_cov_20.876331"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          715 a         1474 c         1615 g          852 t
ORIGIN      
        1 gaagtcctgc ggcgtgtagc gcgacagcgc cgaacggcag aagtgcgggt tctcgctgag
       61 gaagttcgcc ggtgtggtgt cgcggttggt gaagttgcag cggccgccgc ccgagatgcg
      121 gattttctcc gcgaccttct cgctgtggtc caccagcagc accttgaggc cgcgctggcc
      181 ggcgatgccg gcgcagaaca gcccggcggc gccagcgccc accaccacca cgtcaaattt
      241 atgggtcaaa caggcctcca gcccaatagg ggcgggcgcg agaagctatg aaagatatag
      301 cggtccggcc cgaccgggac acaggagtgt agccgggcct acccggcgcc cgtggcgctc
      361 aggcggcgcg gaactgtgcc ggagcggact gcggaaattc gcccgcggcc gccacgccgc
      421 tgatcacatc gccgacgacg atgaccgacg ggctggccag gccggactcg gcgattgcgc
      481 aggacatttc gcccagcgtg gtggtgatgt ggcgctgcgt tggtagcgag gcgttctgga
      541 tcacggccac cggcgtgctc gctggcaggc cggtcagcag ttcgtcctgg atgcgcccgg
      601 cgccgctgac acccatgtag atcaccagcg tgagtttggc cttgtacgcc gtgtcggcca
      661 gtgcgcgcca gtccgtgcct tcgtcgcccg gcttggcgtg gccggtgacg aacaccacgc
      721 cgtgtgcatg ctggcggtgc gtgagcggca cacccagcga agtcactgcg gccaggccgg
      781 cggtgatgcc gttgacgaca tggacctcga tgccggcggc ctgcaggtgt tcgacctctt
      841 cgccgccgcg gccgaagatg aaggggtcgc cgcccttgag gcgcacgacg ttttcacctt
      901 cgcgcgcggc catgatcatc agtttttcga tgaactcctg cggcgtggat ttgcagccgc
      961 cgcgtttgcc gacatgcacg atgcgtgcac cgggcctggc ataagccacg atctcgtcgc
     1021 tgaccaggtc gtcgaccagc agcacggtgg cggcctggat cgccttgagc gccttgagcg
     1081 tgagcagttc cgggtcgccc gggcccgcgc cgaccagggt gcaccggccg ttggaagaga
     1141 gcttgttgtt gttgttgctt gtcatagttg caatgtcagt tgcaagatat gttccacctg
     1201 ccgcgctatc gatgccggca cggcgtggga gcccgacatc acggcgcgga tataggcggc
     1261 ggtgctgtca ctgtcgatgg cagcgggcag gtccggcacg cttgccaggg tgcccggctg
     1321 cgcttcctgc aacagggtgc gcttcccgcc cacaaaggct tccatctgcg gcgtgcggcg
     1381 agcatcggcg acgacctcgc cctcggtgcc acgcagcagc agggcgttgg ccgcgatcag
     1441 ctcgaaagtc tggcccatgg agatcgcgta ttcgggatgg gtgtagctgc tgaccaccag
     1501 cgccttgccc aggcaggggt tcatgatctt gaccaggctg tgggccgggt tgcgcaggcc
     1561 gacggcacgc cgcacatcga gcaggcgttt gagccccggg cacagcagct cggtgggggc
     1621 aaacaccacc tcgccatctg ctattttttt aatagcggtc agcgcaggta tgtcaagggc
     1681 tgcaagcaca tttgacgtga aaacccggct ggattcggtg gccgtgccgt gcaccagcac
     1741 cggcaggcct tcgcgcgcca gcagcaacgc cagcagcggc gtcagcaccg gcagcttgcg
     1801 cgcgccgttg tagctcggca gcaccacgac gggccggtcg cttgccggaa aacgcaacat
     1861 gcgttggtaa gtcgcgtcca ggaaaccggc catctcttcc ggtgtttcgc ccttgatgcg
     1921 catggccagg caaaagccgc ccacctcgag gtcggtcacc gtgccgtcca gcacctggcc
     1981 gaacagatcg gccgcctgct cacgcgtgag cggcctggcg ccgtctttgc cgcggcctat
     2041 gaccttgatg tactgagcga tggacatgcg tgattgtcgg tgaaggcaga taacttcccg
     2101 ttatgagctt aagccggtgt ttgtcaggcg agcaccttga tcgggatcac ggtgcggccg
     2161 ccggtggcgg cccgggcctg ggcagtggtc cggatgatgc gtttgatctc gggcacgcag
     2221 gagccgcagt tggtaccgca tttgagcttg tcctgcagca ccagcagccg ttcgtcgtcg
     2281 ctggccaggt agccggcgcc gccgaccgcg tgggtgatgt ccgtcaggca ggccccgatc
     2341 tcggcatcgg tgacgctgaa acaggtgcat accggtttgc cgcgcgactg cagggccacc
     2401 ggggccttcg cgcccggcag cagcagcagg cggccataac tttgcgccgg cagttcatcc
     2461 tgcagcaggg tcttgatcca ggcttgggcg ctcacatcgc cggccagcac gaagcccgcc
     2521 agttcggcct gctcgccatg gcgcaccagc cgtgcggtgc ggtgctggcc cttcttcctg
     2581 tcggcataac gcaggacatc cgtcccgccc aaaccgagaa tgccctcgat cttttccagc
     2641 acgccggccg gcggcgcctc gaaagccgcc gcgcgaaaaa gcacgccatt gcgctgccgg
     2701 cccgcttcgc ccagcggcac ctggttggca aacggcacgc agctgctgaa ggcaaaggcc
     2761 gccatcagtg gtttgagctg ttcgcgcgcc gccagcactt cgtcatcggg cagccaggcc
     2821 atggccagca gcgaccaggg cagttcggcc ttgaggatct tgaccgccgc gtgtttgagt
     2881 tcgggctgct tggagctcgg gcaatagacc gaggtggtca gcgcgttgat gccggccagc
     2941 ggttcgccgc tgctcgacag gccgctcagg tactcgctgc cccagtgcat ggccatgaag
     3001 gcttggctca ccgccacttc gggtgaagcc tgcagcggca ccacgatgga gccgcgcctg
     3061 gacgtgacgt acaccaggtc gccgtctttg agcaggcggc gcgccatgtc ctgcgcgttc
     3121 atctggacgg acggctcggc cacatggccg aacaggcggc ccagcgtgcc ggtgcggctc
     3181 atgccgtgcc actggtcgcg caggcggccg gtggtcagcg agaaggggta acgggattcg
     3241 cgcggttcgg ccaccggctt gtagaccgtg ttgacgaaac gcgccttgcc gtcgtcggtc
     3301 gggaaaatgc cgtcttcgta cagccgcgcc ttgccggtgg tttcaccttc gggtagcggc
     3361 cactgctgcg gtgcggcttc gagcatcgcg taactcatgc cagtgatgtc gaggtcacgg
     3421 ccgcgagtgg attcgcggtg ttcgttccag atggcctcca cgcttgccgc ctctgtatcg
     3481 gcggaccagg ggaacaggct gctgattttc tgcaccggca acaacttttc cagccgctgc
     3541 gcaaacgcga cggcaatcga ccagtcgtgg cgtgtttcgc cgggtacggg caccgccggc
     3601 cgcacccggc tgatgcggcg ttcgctgttg gtcacggtgc cggttttctc gccccaggtg
     3661 gtggccggca gcagcaggtc ggcgtagtcg caggtggcgg aggtggcaaa ggcttcctgc
     3721 accacgacca gttcggcgcg ctgcagcgcg cggcgcacgg tggcctggtc gggcatggac
     3781 tgcgccgggt tggtgcaggc gatccacaag gccttgatct cgccgtcggc cgccgcctgg
     3841 aacatctcca cggcggtttt tccgggctgg tccgggaccg aaggaatgcc ccagagcgcg
     3901 gcaacctcgg cgcggtgctg cgggttggcc aggtcacggt gggcgctgag cagattcgcc
     3961 aggccgccga cttcgcgtcc gcccatggca ttgggctggc cggtcagcga gaaaggtccg
     4021 gcgcccggtt tgccgatctg cgcggtggcc aggtgcaaat tgatcagcgc cgcatttttc
     4081 gcggtgccgc tgctcgactg gttcaagccc tggcagtaca ggctcagggt ggcggccgag
     4141 gtggcgaaca gcttcgcggc ggtgaacaaa tcgtcttttg tgatcccgca gatctgcgac
     4201 accaggtcgg gcgtgtagtc gcgcaccgtg gttttcagcg cgtcgaaacc gttggtgtgt
     4261 tctgcgatcc aggccggttg ggcccagcct tcccacagca tgatgtgcag catgccgttg
     4321 aacagagcga cgtcggtgcc gggctgcacc ggcaggtata gatcggcgat gcccgcggtg
     4381 tcggtgcggc gcgggtcgca gaagatgatt ttcatcgccg ggttggcggc cttggcgtcc
     4441 tcgatgcgcc ggaacaggat ggggtgggcg taggccgtgt tgctgccgac gatgaagatg
     4501 cactgcgcgt gtttgacgtc gtcgtaacag ctgggcggtg cgtcggcccc cagggtctgt
     4561 ttgtagcccg ccaccgcgct gctcatgcac aggcgcgagt tggtgtcgac gttgttggtg
     4621 ccgatcaggc ccttggcgag tttgttgaag acgtag
//
LOCUS       DAIDFG010000249         4650 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_249_length_4650_cov_17.769097, whole genome shotgun sequence.
ACCESSION   DAIDFG010000249 DAIDFG010000000
VERSION     DAIDFG010000249.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_249_length_4650_cov_17.769097"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          766 a         1549 c         1498 g          837 t
ORIGIN      
        1 ctaccgcctg tcacaggccg aatgcgtcaa atacctggtg caactcgatc acatcggcat
       61 catcgagctc aagccgcaga accgctaccg gctcaaactc gccaagacct tccgctggcg
      121 gccgcacggg ccggtgatgg actacttcag ggacaacgcc ctgctggact acttctcggg
      181 cggttttgac ggcggcggag aggggctgct gctggtgcat ggctccatca gccgcagcct
      241 cgcgccgtcg tttctcgaac gcatgcagcg tgtcgcgcag gattttgcgc agcaacacca
      301 ggccgaccag aagctcgccg agaaagaccg cgaaggttac accctgctgc tggcgatgcg
      361 cagctgggag ttcgaagcgt ttgcggcctt gcggcgataa attgggcaca cccctgttgc
      421 cgctcactgc gtgtagcggc ctctcccctt gcgggtgatt gcgcctgcaa actggcggag
      481 ccaaatctgc ggtccctgct gcgagtgggc catggcgcag tcccccctca aaaagcgacg
      541 cttgtcactc cggggcggta aacaccgtca gcacgccggt gtagccatag aggttgtggc
      601 gtgcgatttc gccgccggca aaaaacccga cgagcggcac gtcgccgagg gcacgccgca
      661 caatctgcag ctcggcgctg ggcgcaccga agtgtgggcc gccgcgcccg gcgcagctga
      721 tgtagatcgc gccggcgatg cgccgggccg ggtgcggggc cgattcggct tcggacgcgc
      781 tcagcgcact ggcgacttcc agcgtcaact cctccggctc cagttcctcg cggatttcgg
      841 cgcagacgcg caccaggtcg gcgcgcgccg cctcggcatt gcgttcgcaa aaagccagcc
      901 tcatgcccgg gctgggcacc tcggcaatgg ccacgccctg gcgcgccggg tccaggccga
      961 tgatgtggcg caccagcaca tcggccccga actggcccgg acgccggatc cggccgtcct
     1021 gcagggcggc gtcagcgctg ctcagcccga ccagcgtggt ccgcactttt tcgagcgcct
     1081 cgcgcggcgc atcgagcgac acaccgaggt cggccagcat cacatcgagc gcgctccggc
     1141 cgtccagttc cgtcaccaca ttggcgtcgc aggcggtgat ctcgtgctcc cttgaaaccg
     1201 gctggcagcc ttgcgtcacg cgcgacatca gaccggcgcc ctgggcaaag gccacaccgc
     1261 tcaggccgcc gctgaacacc ccgctggcgg caccctgccc cttcacattg ccgctgccgc
     1321 tgagtgcgaa ctggaccgcc ttgctgcggc tcgacgccag cccgccaaag acatacccgc
     1381 tgctggtgcg ctgggccatc tcggcgatca gctcggccac gtcgggggtg ctggcgtcgg
     1441 catgcaccag cgcggtgtgc gcctggaaat ggctggcacc gggcggtggc aggggcgaca
     1501 cgccggaaaa cacgcggtac tggtccgacg gcagctcgca cagcatcacc gccagcgccg
     1561 gctcgtcgaa atactcgaca ttgttcgacg cgatgccgat gccgaccgtg cccgcccagt
     1621 cggtgatttc aggcagctcg gcgcccaaaa aatccaggat gtcctgcgcc tgcgcggtgt
     1681 agtggtcggt gatgtaaagc aggcccaggc cgggcgaact ggcataaccg tgcagggcca
     1741 tctgcgcgcg caactgggcc agcaccaagc cggccgccat gcgccattgc gggtgggtgg
     1801 cgtggccgta ggggaaaagt ttcatggtgt tgtcacggta ggttggcgct ggcacactgc
     1861 ctcaccactt gggcagcaat cgctctccgg gaagcacatc gtatggcgaa gcccagccgg
     1921 gcaacccacg caggcgcgca agccacgcct cgatgtgggg gtatcggccg gcgacctcat
     1981 agccgctttc ctccagcgga taaaacaagt agccgcacag cgagaagtcg gcgatggtgg
     2041 ccgccccgcc gaccatccac tcgcgcgtcg ccagatgttt gtcgacgatg gcaaacgcgg
     2101 cgtccatccg cgcgcgcagc caggccatca cggccgggtc gggtgcggcg gggccgaagg
     2161 ccttcatgaa gcggtaggtg gcgaagtaac tcgtgaactt gtggttgtcg aaaaacagcc
     2221 agcgcagcac ttccagcctg tcatcttccg tggccccttc atacgcgcca tgtttcttcg
     2281 cgaggtagcc caggatgacg cccgactggg tgaggcggcg cgtgccgtct tccagcaccg
     2341 gcgcctcgcc catctcgttg gtctgctcgc gccagggccc ggtgcgcgtg acgcctttca
     2401 tgaagtccac aaagacaggc tcccagggct ggcccagggc gcgcagcagg aatgccacct
     2461 tgaaggtgtt gcccgactgg caaaaaccat gaagacggta catgggaacg ctttctcgct
     2521 ttctttcagg tgactgccag ccggccaccg gcccggcagg gtttcaacgc gtggtcttgc
     2581 gcgccgcccg gcgcaccggc gccttggctt tcggcttcgg tttggcccgc gccgccgttg
     2641 tggccgccgc cggggtgggc caggcctggc tgctgcgcag ggcgctgtcc atcgccgagc
     2701 gggcgccccg ggccacggtc ttgccggcgg agttggccat gcccttggcc aggtccgtgg
     2761 ccatgccggt ggcggttttc atcgcgtcct tggccaggcc ggtggccatg ttcttcgtcg
     2821 tgctcatggc ggtttttttg gcggcgtcct gcatggccgt ggtggcgatg gtctggaact
     2881 gctgcgtcag cgcgccccac cactgcatcg ggtcgacgac gccaggcgca gcttcggcgg
     2941 ctttttcagc cttgggcttc gaggttttgg ccttcgcggg cgtttcactg ttctggtaac
     3001 tggtgggcgg aacctccagc cccgaaaacg tcttggcctt gtcggctact ttctgcacgc
     3061 cgccagccac cgtatcggcg accttgagtt tgaaagcgtt ggcaacgtcg cccatgttga
     3121 aattcatgcc cttgagcgtg gccagggtca tcttctggac ttccagcgcc tggatggtgg
     3181 ccttgagcgc catggcgttc tggtccagcc agaactggac ggccttgagc tcctcgatgc
     3241 gtttgtcgag ctcctcgaca ttgagcgtcg gcgcgaccca gttgcccagg ttgggcaact
     3301 ggggaatgct ttgcgacgcg ctgccggcgg cctgcttggc aaggttctgc aaaaagtcaa
     3361 agcccggaac cagtttgccg aaaccggcga aggatgggtt gtctgcgtcg ctcatgctgt
     3421 ctcctggtgt tgtaggacgt gcccgaagcc ctgatgcaac actgcacctg tctgcggttc
     3481 acggtgcttt cattgtgcag ggtcgtggcg gcgcgtcaac ccgttttttt gcatacctct
     3541 ggcgcagcgc acctgcccgg gccgctcacc agctgcttcg tcctcacctg cgaatcttca
     3601 agtaaaatca gccgcaaacc cttgactgac aggcgtcaca agctattgat ttaatagcaa
     3661 atcaggcttt cggagcgtaa gaaggcgggc ggcgctcacg caggctggcc acaccttcgc
     3721 gcacgtcggg gccggcaaaa cccatgaact ccagcgccag cgaggcgtcg aaggccggcc
     3781 cggcctggcg cagccagttg ttgagcgcgt atttggtcca gctgatggcg gttttcgagc
     3841 cggcggccag ccggtcggcc acctcgtagg ccttgggcag cagctgctct tcgtccactg
     3901 ccagcgacac caggccgatg cgttcggcct cttcgccgct gatcggctcg cagagcatca
     3961 ggtagtactt ggccttggcc atgccgcaca gcaggggcca gatgatggcg gcgtggtcgc
     4021 cggcagccac gcccagccgc gtgtggccgt cgacaatttt ggcgcttttg gcggcaatcg
     4081 aaatatcggc cagcaggcct gccaccagac cggcgccgac cgccggcccg tgcagcgcgc
     4141 tgacaatcgg cttgtcgcag ttgatcacgt tgtagaccag gtcgcgcgct tccttccaga
     4201 cgcgggtacg ggtttcaaaa tcctccgcca tgtcctgcac cagcgcgaga tcgccgccgc
     4261 cggaaaaacc caggccttcg ccgcgcaaca cggcgcagcg caccgagtcg tcgctgccca
     4321 cgtcgcgcca gatctgcgca agttcccggt ggccgtcgtg cccggccgtc ggcaacttgc
     4381 cgttgagggc tttcatctgg atatccagca ccgccccgcc gacgccgcgg cgcgtgatgt
     4441 gaagggtctg gtaggcgctg taatccatag tctgggcagt catggtgtct ccgtcaggtg
     4501 gcaaaaggga tccccgaatt gtgccctgca cggacccgga gccgcccgct tgaccttcat
     4561 caaccggggg catgacgccg tggcgcatga tggaggcgaa tacaagaagg aatgacccat
     4621 ggacaaacaa cacagcgcac cctaccaaca
//
LOCUS       DAIDFG010000250         4626 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_250_length_4626_cov_17.261868, whole genome shotgun sequence.
ACCESSION   DAIDFG010000250 DAIDFG010000000
VERSION     DAIDFG010000250.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_250_length_4626_cov_17.261868"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          718 a         1488 c         1557 g          863 t
ORIGIN      
        1 tctcttggcg aagcaagaga aagtgagtcg cccgccgggg cgagacccgg cttgcaggcg
       61 taccttcacg ctggaggtgc tcagtagttg ctatgaatta aatagctgcc agcgcgcgca
      121 caccaagggc tgtagccaga aatcatcctc aagagcggga agccttggct ttccggtaat
      181 tccaccaagg caacatcgcc cgcagcgaca gcaccttgcc gctttgtgcg tggtcgccgc
      241 tgtagccggg cagtccggcc agcgtcctct gccagtcgct gctctgcaac tccttgagca
      301 gggcctgtac ttggggttgc ggcagttcgg acttcaggca gaccaggtgg taacgctcct
      361 ggaccagcgg cacaaacccg aggtttttct cgtgcgctgc ggcttcgatg cccatcccgg
      421 catcggcggc gccgctggcg atggcctgcg cgaccgcgct gtgggagggc tcctgcgtgt
      481 tgtagccttc gatgccgtcc gcgttcaggc cggcctgcgc cagcagttcg tcgagcagca
      541 cccgcgtgcc ggtgcctgcc gcgcggttga catatcggat gcctggccgc ttcaggtcgg
      601 cgatgccgct gatggccagc gggttgttct tcgccaccat caggccctgc cggcgatgcg
      661 cgaaaccgat cagcttgtgc aggccgggct tgagcaggct gcggtaagtc agcgccgcca
      721 ccgacttgtt acccggctgc tccagggtgt ggaagccggc catcatgcac cgcccggcgt
      781 tgagcgccgc aatcgcgtcc acgctgccca tgaagcggat gtccaggtgc aggccgcgcg
      841 caccggcctg ttcgcgcagt gcgctcagcg cgctgtcgtg gctggcatac agcgtgagca
      901 cgtgggcctc atcgtcaaag gcggtggaga aggcccgttc gatgtcgccg cgcagcgcct
      961 cgatctgcgg cgccagccgg gcctgcgcct ggcgctcggc ccacaacagc ttctcgccaa
     1021 actcggtgag ctgggcgcgc tggcccttgt cccagacgat cagcggacgc tccagtgtca
     1081 gttcccactt tttcagcgcc ccccagacat ggcggtagga cagatcgagg gcgcgcgccg
     1141 ccttcgaaat ggagccctgc tcgcgcacgg cgtgcagcag gtccatcagc ggattgcgga
     1201 tcagcgtgtc cttgctgcgc tcgggcgtca acagatagga gagttcgact ttgtgcatgg
     1261 ggtggcagaa gggtgtgctg attatggtcg caaggccggg cgcgtctttg ctatgcaatt
     1321 tgcttcatag ctcgtgattc ccgatggcga cccacggcct tcgcttctac gataaggtca
     1381 caactttttt cctgcatgaa cactttcccg gacagcgtcg gcacggcgtt ttcgctcatc
     1441 gtttccctgg atcccctgtt ggcgagcatt gtgttccgct cgctggcggt cagcgcctgt
     1501 gcctgcgcaa tcgcctgcag cgccgggctg ctgctcgggg cctggctggg cgtggcgcgc
     1561 tttcgcggcc gcggcattct gctgaccgtg ctcaacacgc tgctggccct gccgtcggtg
     1621 gtggtcgggc tggtggttta tttgctgctg tcgcggtccg gcccgctggg ttttctcggc
     1681 tggctgtttt cctttcaggc catggtgctg gcgcaggccg tgctggtgct gccggtggtg
     1741 acggcgctgg tgcgccagac cattgaagac gccgaccgtc tgcacggcga acagttccag
     1801 tcgctgggcg caggcaaggg catgcgcagc ctgctgctgc tgtgggacga gcgttatgcg
     1861 ctgctcaccg tgctgatcgc cgccttcggc cgggcgatct ccgaggtcgg cgcggtgatg
     1921 gtggtgggcg gcaacatcga cggtttcacg cgcgtcatga ccacggcgat tgcgctggaa
     1981 accagcaagg gcgacctgcc gctggcgctg gcactgggcc tgctgctgct ggccgtggtg
     2041 ttgctgctca acgtgctgat cgcgctggtg cggcgctggc gcgagcggct cgatgacggg
     2101 ctgcccgaaa cggcccaatt gctggtcccc gaggctggag ccgtcaaatc atgagcgcgc
     2161 tgttcgaact caaagcggtc gacgtgcact tcggccagac ccgcgcgctg accggttgcg
     2221 cgctcagcat ccgcgccggt gaacgggtgg ccctggtggg tgccaacggc agcggcaaaa
     2281 gcacgctgct cagaaccctg cacgggttgg tgccgccgac gcggggcacg gtgtcttcag
     2341 acgtttcggc gcgtcaggcc atgttgttcc agcggcccta catgctgcgt gccagtgccc
     2401 tgaacaacgt ggcgctgggt ctgtggctca aggccgtgcc ctggaaaaag gcgcaaaccc
     2461 aggcgcttca ggcgctggcg cgtgtcgggc tggcgggcat cgcccaccgc aatgccaagg
     2521 ccctgtcagg cgggcagcag cagcggctgg cgctggcgcg tgcctgggcc ttgcagcccc
     2581 aggtcctcct gctcgacgag cccaccgcca gcctggaccc cggcgccaaa cgcgacgtgg
     2641 aggccctgat gaccgagttt gccgacgccg gcatgacgct ggtgttcagc agccacaacc
     2701 tgggccaggt caaacgcctg gccagccgcg tgatctacct cgagcacggt cgcctgctgg
     2761 ccgaccggcc cacccatgat tttttcaacg gccccctgcc gctggaagcc gagatgtttg
     2821 tcaaaggaga acttgtatga agcccacccc cgcacggtcc cggatgttca agtgttttcg
     2881 ggttgcagcc ctttttccta ttgcgctggc agctactgtt tctatagcgc agcaaaacac
     2941 catcgtgatg gcctcgacga cctcgaccga gcagtcgggc ctgttcggca cgctgctgcc
     3001 ggccttcaag caggccagcg gcatcgatat caaggtggtg gccctgggca ccggccaggc
     3061 catcgacatg ggccgccgcg gcgatgccga cgtgctgttt gtgcacgacc aggtggccga
     3121 ggaaaaagtc attgccgaag gttttgcgat caaacgctac ccggtcatgt acaacgactt
     3181 cgtgctgatt ggcccggctg ccgatccggc gaaaaccaaa ggcaaggaca ttgttgaggc
     3241 actgaaaaaa atcagtgccg ccaacgccac ctttgtttcg cgcggagaca agagcggcac
     3301 ccatgccgcc gagcttcgtt actggaaaaa tgccggcgtc gacaacccgc agtttgccgg
     3361 ctacaaggcc tgcggctgcg gcatgggccc ggcgctgaac atcgcggcca gctcgggcgc
     3421 ctacgtgatg gccgaccgcg gcacctggct ggcgttcaag aaccgcgctg atctggcggt
     3481 gctggtggag ggtgatacac gcctgttcaa ccagtacggc gtgatggtgg tcaaccctgc
     3541 caaacacccg aaagtcaagg tggccgaggc gcaaaaattc gtggactggg tgatttcacc
     3601 ggccggccag gcggcgattg ccagctacaa gattggcggc gagcagctgt tctttccgaa
     3661 cgccacgaag tgatggtgaa ctggcagcgc gtcctggcaa ccatggccgt gtcgctggtg
     3721 gcctccgcag cgcaggcgca gcctgtgccg gttcagctct acgcggcggg cagcctgcgt
     3781 gaggccttga ccgaagtggc gcagcagttc gaggcgcaaa gcggccagaa ggtggcgctg
     3841 acatttggcg cctcgggcct gctgcgccag cgtatcgaaa agggtgaacc ggcccaggtc
     3901 ttcgcctcgg ccgatgtgga ccatccgcag cgcctcgcac aggccggtgg ctggcaggca
     3961 ccccgggtgt ttgcgctcaa ccagatgtgt gcgctggcgg cgccttcggt cgatgtcagc
     4021 ccggatgcgc tgctggagac catgctgcgc cccggggtcc gcgtcggaac ctcgacgccc
     4081 aaggccgatc cgtcgggtga ctacgcctgg tcgctgtttc gtcgcgccga cacgctgcgc
     4141 cccggcgcat ttggggtgct cgaggccaag gcgctgcagc tcacgggcgg tgccgactcg
     4201 cccaaggcgc cggcgggtcg cagcacctat gcttgggtca tggatgaagg caaggccgac
     4261 ctgttcctga cttattgcac caatgccgtg gtggctcagc gcgaggtgcc gcgcctgaaa
     4321 atcattgcgg tgccgcccgc gctggaggtg gcggccagtt acggattgac ctggcgcgcg
     4381 gacggggatg gcgccgccac gcggctcgcg cagttcatcc tgtcgccgcc cgcgcaggct
     4441 gtcctgcagc gtcacggttt cggccggcct tgaggatggg cctggctggt ttttccgccg
     4501 ggcctcgcca tccttcgaca ggcgcaggat gatcggatca gacgtgtgcg cctcgaagaa
     4561 cttccccttc aagcaggggc cgcaggcgca gcgccccctc ggggctggag cctgcggctc
     4621 caaacc
//
LOCUS       DAIDFG010000251         4615 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_251_length_4615_cov_18.612500, whole genome shotgun sequence.
ACCESSION   DAIDFG010000251 DAIDFG010000000
VERSION     DAIDFG010000251.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_251_length_4615_cov_18.612500"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          855 a         1482 c         1449 g          829 t
ORIGIN      
        1 ggcggagctg gccgtcaagc ttggtcctcc cgagtacttt atgctgatgg tgctggcctt
       61 caccaccgtc agtgccgtgc tgggcaaaag cacggtgcgc ggcctgaccg ccctgtttgt
      121 cgggctcggg gcgggcctgg tcgggctgga ccagatttcc ggccaggcgc gttacaccgg
      181 tggcgtgccc gagctgctcg atggtgtcga aatcgttctg gtggccgtcg gcctgttcgc
      241 tgtggccgaa gccatgtacg ccgtgcttta cgagggccgc gtggtcgagt cgcaaaacaa
      301 gatgagccgc gtccacatga ccggccggga ctggcgccgt tcctggccgg cctggctgcg
      361 cggcaccgcc atcggcgcgc cgtttggctg catacccgca ggcggcaccg agattccgac
      421 cttcctgagt tacgccgccg agaagaagct ggcgaaagaa agcgaccggg ccgagttcgg
      481 cacggccggc gccatcgagg gggtagccgg ccccgaggcg gccaacaacg ccacggtcac
      541 ggcggccatg attccgctgc tgacactggg catcccgacc tccaacacca cggcgatttt
      601 gctgggcgct ttccagaact acggcatcca gccggggccg cagctgttca cctcgtcgtc
      661 ggccctggtc tgggcgctga tcgcctcgct gtacatcggc aatgtgatgc tgctggtact
      721 caacctgccg atggtaggcc tgtgggtcaa gctgctgaag attcccaaac cttacctgta
      781 tgcgggcatc ctgatttttg cgacggtggg cgtctacggc atgcggcaaa gtgcgtttga
      841 cctgttcctg ctgtatggca tcggtgtgct cggggtggtg atgcggcgtt ttgatttccc
      901 gaccgcgccc gtcgtggtcg gcatgatcct gggcccgctg gccgaagcgc agctgcgcaa
      961 cgccatgtcg atcggcgagg gcagcgcgat ggtgtttctg cagcggccca tgtcggtgat
     1021 cctgatcgtg gtggtgctgg cggtgctgat cctgccgcgc ctggccaaac gctggtcggc
     1081 gcgccggctc gcggcctgag gtcgatgttt atcataaaat aggactccag cccaatatcc
     1141 acgggcgtaa gcagctatca aaagaggagt agtgtgcgtc ttgaccggtg tgtgttgcac
     1201 ctgaccggcg gcagtggccc aggcgccgcg ctaccatcgg cgcatggaac acaccgacac
     1261 cacggcgcac ggtttgccgc tcgatgcgca gagcattctg gagctggccg caaggtccat
     1321 gttccagctg ttctccagca tcagccaggg catgttcctg gtggaccgca ccggccgcat
     1381 tgtctgggtc aacgacggct acaagcggtt tttgccggcg ctgggttttt cgtcggtgga
     1441 ccagtttgtc ggccacatgg tggaggaggt cattcccaac acgcagatgc gccgcgtgct
     1501 ggaaaccggc gagcctttcc tgatcgacct gctgaccaac cgcgccggca cctttgtcgt
     1561 cagccgcatc ccgctgcgcg atgaaagcgg cggcgacgtc atcggtgcca tcggcatcgt
     1621 gctgttcgac caccccgaga ccacactgca gccgctgatc agcaagtttg cgctgctgca
     1681 ccgcgacctc gatgacgccc gccgtgaact ggccagccag cggctgcagg cgccgggcca
     1741 gcgccagtcc aaatacacct ttgccagttt tgtcggcacc agcccggccg cggtcgaggt
     1801 caagcgccag gcgcgccgcg ccgcccagac ctccagcccg gtgctgctgc tcggcgaaac
     1861 cggtaccggc aaggagttgc tggcccacgc catccatgcc acctccagca gggcgcgcgg
     1921 gcccttcatc agcgtcaaca ttgccgccgt gcccgacacc ctgctggagg ccgaattttt
     1981 cggcgtggcg cccggtgcct ataccggcgc cgaccgcaag gggcgcgacg gcaagttcaa
     2041 gatcgctgac ggcggcacgc tgtttctcga cgagatcggc gacatgccgc aggccttgca
     2101 ggccaagctg ctgcgcgccc tgcaggaggg cgagatcgag ccgctgggct cgaacaagct
     2161 gattccgttt gacgcgcgcc tgattgcagc cacttcgcgt gacctgggcg cgctggtgcg
     2221 cgagggcaag ttccgcgaag acctgtttta ccgcctcaac gtgctgccga tcaaggtgcc
     2281 gccgttgcgc gagcgtcgct ccgacattcc ggcgctgctc gaagtgctgg gcgaggaaat
     2341 ggcgctgcgc agcggcgacc gccagcccga actggcgccc gacgcgctgg ccctgctgtc
     2401 ggcgcaaacc tggcgcggca acatccgcga actgcgcaac gtgctggagc aggcggtgat
     2461 gcgcagcgac tcgccgagca tcggccgcgc gcagtttgag gaggtgctca aggaggcggg
     2521 catcgagcgt attgcacctt tgccgctgga cgggctgggg gacagcgcag agtccgcctc
     2581 cgatccgctg cgcccgctgg ccgagcaggt ggcacaagtg gagcgccgcg ccattgccgc
     2641 cgccatggcg gcgaccggcg gcaacaagct ggcggcctcg cgcctgctcg gaatttcgcg
     2701 cgccaagctg tatgagcgtc tggataaccc tgtataaaaa aaatgcaatt gcatgataat
     2761 aagtcaataa aaatgactta taaacagaca aatcaatgtt gatcctgaaa aagcctatat
     2821 agatcaacgg tctttttctg gcacgacatg tgcaatcatg gtgcatcgcc aaacaacagg
     2881 agacatccat gcatcgtcgt accctcatcg cgcttgccgc actcgccacc gctttcgttt
     2941 cgccactggc cctgagccag gccaaagaaa tcaagatcgc catcattgcc agcaagacgg
     3001 gtccgctgga ggcctatgcc aaacagacca tcgttggctt ccacatgggg ctgaactacg
     3061 ccaccggcgg caccatgatg gtggcgggca agaagctcgt ggtgatcgag aaggacgacc
     3121 agggcaagcc cgacgtgggc aaggcggcgc tggctgcggc ttatgccgac gaaaaggtgg
     3181 acatcgccgt cggccccacc gcctcgccgg tcgcgttggc catgttgccg gtggctgagg
     3241 aatacaagaa gatcctgctg gtcgagcctg ccgtggccga cagcattacc ggcgacaagt
     3301 ggaacaagta catcttccgc accggccgca attccagcca ggatgctgcc gccaatgccg
     3361 tcgcgctgga ccggccaggt aatgtggtcg cccttctggc caacgacaac gccttcggtc
     3421 gcgatggcgt gaaggccgcc aagtcgttca tgaagaacgc caagatcgtt cacgaagagt
     3481 acctgcccgt gggcaccacc gacttcacgg cgggcctgca gcgcattgtc gacaagctca
     3541 aggaccagcc gggcaacaag tacctctcgg tcggctggtc gggcagcccg acgccgtttc
     3601 cgaagatcgc cgatctggac cttgaaaagc gctacggcat caagctcgcc accggcggca
     3661 atatcctgcc cgccatggtc gcctacaaaa gcttcccggg catggaaggc gcgctgtatt
     3721 actacttcgg tatcccgaag aacccggtga acgaagcgat ggtggcccag cactaccagc
     3781 agttcaagac accgccggac tttttcacgg cgggcggttt ctccgcagcg atggccgtgg
     3841 tgacggcgct caccaaaacc ggaggcgaca ccaataccaa caagctcatc tccaccatgg
     3901 aaggcatgag ctttgacacg cccaagggca agatgacctt ccgcaaggaa gaccaccagg
     3961 ccatgcagag catgttccac ttccgcatca aggccgaccc ggcatttgcc tggggtgtgc
     4021 cagagctggt tcgcgagatc aaacccgaag agatggccat ccccatcctc aacaagcgct
     4081 gatgcgctga tcggggaggg cgctcgccag agttggcgag tgcctgccct ccttcactcc
     4141 tgttttgata gctgctggtg cttgatccgt aagcgccggg ggctgctttt accccaaaaa
     4201 tgacctggag cctggtgctg aacccatgct ggcaacccac gaactaacca tccgctttgg
     4261 tggccacgtg gcggtgaatg ccgtgacctg ctcgttcgag ccaggcacgc tcaccgccat
     4321 cgtcggcccc aatggtgcgg gcaaaacgac ctatttcaat ctgatctccg gtcagctcaa
     4381 ggccagcagc ggcacggtca tgctcaacgg cgaggatctg tcgggcaaaa gcgcctcggc
     4441 acgtacccat gccgggttgg gccgggcctt tcagctgacc aacctgtttc ccaacctcac
     4501 cgtgctggaa aacgtgcgtc tggcggttca ggccacacgc cacggtgcgc accggcacgg
     4561 cctgaacctg tggagcatct ggagcgacca caaggccttg ctgcagcgcg ccgaa
//
LOCUS       DAIDFG010000252         4541 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_252_length_4591_cov_14.675485, whole genome shotgun sequence.
ACCESSION   DAIDFG010000252 DAIDFG010000000
VERSION     DAIDFG010000252.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_252_length_4591_cov_14.675485"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          781 a         1380 c         1499 g          881 t
ORIGIN      
        1 acctggcctc cggcctgctc cgcaaggcac tggatgcgtt tgcccggctt gatgtcaatg
       61 tggccgtgtc catcctcaag gaagacgatg ccatcgacaa ggagtttgac ggtttcgtgc
      121 gaaagctgat cacctacatg atggaagatc cccgcaccat ttcgtccagc ctggacctgc
      181 tgttcgtggc caaggccata gagcgtatcg gtgaccacgc gaaaaacatc gcggagttca
      241 tcatttacgt ggtcaagggc gccgacgtgc gccacacctc catggaggcg atagagtccg
      301 cggtccgctg accatggatc gctgcttatg aagactgtac cccgcgtatt ggtggtggaa
      361 gacgagccgg cgatcgccga gctcgttgcc gtcaacctca ggcacaacgg cttccagccc
      421 atctgggcgg ccgacggcga tgctgcccag cgcgagctcg atgccgtgtt gccggatgtc
      481 atcctgctgg actggatgct gcctggccac agcggtctgc agctggccaa gaaatggcgc
      541 gccagcgcgc gcaccaaggc catccccatc ctgatgatca cggccaaggg cgacgagccg
      601 gacaagatcg ctggtctgga tgccggtgcg gacgactaca tcaccaaacc tttttccacc
      661 caggagttgc tggcccgcat ccgcgccgtg ctcaggcggc gtgcacccga gaccatgaat
      721 gacagtgtga cggtgggagc cctggtgctc gatgccgcca cacaccgcat ttcttttgag
      781 aacgaagagc tcaagctggg gccgacggag ttcaagctgc tgcactacct gatgaaacat
      841 gtggagcgcg ttcacagccg gggccagttg ctcgacaagg tctggggtga ccatgtgttc
      901 attgaggagc ggacggtgga tgtccatgtc aagcgcctgc gcgaagccct gggccctgcc
      961 ggtgcactgg tggaaacagt gcgaggcgcg ggctaccggc tgaccgctcg tccccgaacc
     1021 tcatgaggga ttgtttttcc cccacaatcc gcgcatgacg tggcgtcttt tccgtttctt
     1081 ccttttccag ctggcaggcg catcgctggg ccggttcctg tttcgcggca gcagccctac
     1141 gcagggcgcc atcctgggct tgctggctgc gagtgcgttg tgggtgctga tagacctgcg
     1201 gcgcggtgcc cgcctgctgc tttggctcaa acgcggcgag acggctgttc cggccagggt
     1261 tgatggcctg tggggtgaag tggccgaccg gacctggcgg ctgatcaggg cgcgggagca
     1321 gaaagcggca gacagcgagg cgcggctgca ggaattcctt gccgccattc aggcttcccc
     1381 gaacggggtg atcctgctcg acaaaaacaa ccgcatcgag tggtgcaacg agaccgctgc
     1441 ggtccatttc ggcttcgacc cgaaaaagga tgtcatgcag catgtcggca atctgctgcg
     1501 ggaccctcat tttgcgggct atttggcggg cgacgacttc ctgcacgaaa tcgtcatcat
     1561 cggcccgcgc agcacggcca gccggcccgt caggctggct gtgcatttgc atcgttacgg
     1621 cgaaggtcgc aagttgctgc tgtcgcgtga cgtcacggcg ctggaacagg cagaaaccat
     1681 gcgtcgcgat ttcgtggcca acgtgtcgca cgaaatccgc acgccactga ccgtgctcac
     1741 aggttttgtg gaaactctgc agaccctgcc gctcgatgac gaggagcgcg cccgttacct
     1801 cgccctgatg gccaggcagg ctgagcgcat gcagacgctg gtcaacgacc tgctgacgct
     1861 gtcgcggctg gagggtagtc cgccaccggg aagcagtgaa tgggtatccg tgcgtgccct
     1921 gctgaccctg tgcgagcagg aagggcgggc cttgtcggtc ctgcttaccg acggaagcgg
     1981 gaaaaagcat gacttgcggg tggaagatgc ggcagacgag gagatcgccg gttcgcaggc
     2041 ggagttgctc agtgcgatgt ccaatcttct caataacgcg gttcgctaca cgccggcggg
     2101 tggccgcatc gaggtcaggt ggcagacgct gccggacggt cgtgccgagt tttccgtcaa
     2161 ggattccggg cccggcatcg cgcctgggca catcccgcgc ctgaccgagc gcttctatcg
     2221 ggtcgacagc agccgctcac gcgaaaccgg tggcacgggg ctgggcctgg cgattgtcaa
     2281 acacgtcacg cagcgtcatg gcgccgaact ccgcatcgag agccaacctg gccagggttc
     2341 gcgcttttcc atcgtttttc ccgccggccg ggtgcgcccg ctccagccgg tctgattcct
     2401 ccggcgggtg cggccttgac cggctgtgtc agaccgtgga gacgatctcg acatcgttgg
     2461 cgtagacgtc gacccgcttg tcgtacaggg ggatggtggt gccgttgacc cggttgctga
     2521 ccagcttgcc caggtccacg tcggcaatca cgatctgctc catgttgggc acaccctcgg
     2581 cagcgatgcc gtcccgcgcg aagggaaagt cggacggggt gatgatcgcg gcctggccaa
     2641 aatgaagacc cagaccttcg acggcgagat tgcccacggt cccggtcacg gctacgtaca
     2701 cctggttctc gatggcgcgc gcatggcagc agtagcgcac acgccagaaa ccctgcctgt
     2761 cgtcggtgca ggacggcacg aagatgatgt cggcgccctg ttcgcacacc tttctcgaga
     2821 gttcggggaa ttcgatgtcg tagcagatca ggatgctgat gcgaccgtac ggcgtttcaa
     2881 acaccttcag ttcgttgccc gggtctccct tccatttctc cttttcccag cgtgtcaggt
     2941 gaatcttgtc ctgggtgaac accttgccgc cgggcgtgaa aagataggcg gtgttgagca
     3001 ggcgtccgtt ggtgatggtc gggtggctgc cgccgatgat gtgaacaccc caatgcgcag
     3061 ccagctcggt gaacagcgcg atgtagcggc ccgtgtagct gtccatttcc cgcacggcgc
     3121 tgcgcaggtc gctggtgtcc atgaaggaca gcagctgggt ggtgaagatt tccggaaaca
     3181 tcacgaactg gggtttgtag tcgccggctg ctttcatcac gaagcggacc tgattctcga
     3241 aaccgtccca gtcgctgatg gcgcgcagca ggtattgcac cgccgcgatg cgtaccgtgg
     3301 tctgctgggg tttgtgcgcg gggtcgcgcg cgaggtcggt gctgctcatg ctgttgcctc
     3361 ctgctgcagg gccgcagcca gctgcagggt cggttttctc gtcgggtcgt agtccgggtt
     3421 gacccaggcg atcagtgctg cgtggccgag agagtcgctg tcttcgggca ggtaattttc
     3481 catgatgccg cagtagcgga atccttcacg cagctgaaag ctcagcacgg ggtccacgaa
     3541 atccccccac agcgtttttt ttgcatagag ctcaatcgac atctgctgcg ccacgacgct
     3601 gtaccccggc agtctgccgc aggcaatgat tcgccggagg ttgagttgcc ggcagatgcg
     3661 gcgccggcct tcgtacaggg cgtggcctac ccgtttgccg cgcaggcgcg gatcgacaaa
     3721 aacttccgcg ccgtacaagg tgaatcctgc cgggttgtgc gtgtcgaagg tgccggcggc
     3781 ggtgatctgg gaccatgtgt gctcgtctgc ccattcgtcc caggcaatca ccagcgagct
     3841 ggcgcagccg acaatgcgct ggtccagcgt ggcgaccagc tgtccttcgg ggaacaggtc
     3901 caactggtgg gccagctgat cgcgccgcca gggggcaatg gtgggataaa catgggcctg
     3961 cagcttcagc aaggcgtcaa tgtctgcggg agaggtcgaa cgaacggata gcttggtcat
     4021 gcatcatttt tcccgagcgt caacgccttc gccagagctt gttgacgcaa acgggcgtag
     4081 gagaagggcc gcattgcccg tagcgcgtcc tgcgcgtctc cttccggcgc agggcggcag
     4141 gccgcctacg gtcgctggcg ggcaggtgcc gtgcccgggt ctgccccctg gccggcagcc
     4201 gccacgctga ctcttttgta cagtgccacg ttttcgtttt tgtcggtggg gcggctcagg
     4261 gtggccaggt aggcccactc tggcatgtcc acggtttcgc tgagcgcgcc ctgggcgtcg
     4321 gcatcgacca ccatgtaggg gcagacggct ttggcgccag cccggcgcag ttccatgtgg
     4381 ccgtggtact ggaaggcggc gacctgggcg ctgttcaggc cgtatacctc gacgcaggca
     4441 ccgggctcga cgcgcgccgc gacctgccgc gacagcggcg cgtagctgcg ggcatagtcg
     4501 agcaggggca gccacagcgt catcagcagc agccagcaca g
//
LOCUS       DAIDFG010000253         4582 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_253_length_4582_cov_18.722774, whole genome shotgun sequence.
ACCESSION   DAIDFG010000253 DAIDFG010000000
VERSION     DAIDFG010000253.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_253_length_4582_cov_18.722774"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          816 a         1517 c         1417 g          832 t
ORIGIN      
        1 atcgggtcgc cttctctttg cttactttct cttggcgaag caagagaaag tgagtcgccc
       61 gccggggcga gacccggctt gctggcaaaa ccgcaccgca agagggaaag acggcttcga
      121 caagcccagc ccgaacagtg attgaactca gatctggatt gctggcgccc gccgggcgag
      181 tcccggcttg caggcacacc ccgtcacccg gagctacatc cgctcagcat ctatttataa
      241 gccaaaaccg cctccaaccc atgccagcaa agcgcaagca gctacaaatt caatagcacc
      301 cgacacattg agccatgcca tagccgcgtt aaactcgccg catggcctgg aacgcaacgc
      361 tctctctcga ctacaccttg caggccagca agacgattgc gcacttccgc cacgacggcc
      421 cgctgcgcat tctgcaaagc ctctaccccg aaggcgacgc cgtgtgccac aacgtgctgg
      481 tgcatccacc gggcggcctg gtgggcggcg acacgctgga catcgcggtc acggcggcag
      541 ccggcagcca cggcctgatc accacgcccg gcgccacgcg cttttaccgg tcagcgggtg
      601 aaaccgccct gcagcgcacc cgcctgcgcc tggaagccgg cgcccgcgcc gaatggctgc
      661 cgatggaggc gctgtgttac agcggctgcc tggccgaaaa ccacctgacg ctggatctgg
      721 cacccggggc tgaactgatg ggctgggatg tcaccgctct cggcctgccc agtgccaacc
      781 agccctttga acaaggcaac ttttgccagc acatcgagtt acccggcgtc tggctggaac
      841 gtgctcgcat ccgggccgac gacctgctgc tgatgaacag cccgctgggc ctggccggcc
      901 agcgctgcat cgccaccttg tttttttccg ccggcagcaa gctcgaccgc cagcgccggc
      961 aactggctct ggatctggcg cggcaggtgc tggaggcgca cccgctgaac gccaccgcag
     1021 gcgccaccag ccccgatgcg caggtggtgg tcgtgcgggt gctggcgccc ctggtcgagc
     1081 cggccatggt cctgctcaaa caagtctgga aggtctggcg ccagcacttc tgggaaaaag
     1141 acgcgcccag cccgcgcatc tggtcggcct gatcgtcacg acggatctgc cgtctgcggc
     1201 ctacaccctg tttgcgggcc gtcctaccgg gcgcgggcac cagcggcgca aaatcggggg
     1261 cggcattgcc ccccatcaac attgatctgc gagacgctca tgaaaacctt tctgacccct
     1321 gccctggtgg ccgttaccct gctgttcacc ggatgcgcca gcaccaccga aggcggtgcc
     1381 gttggtgcgt cgcgctcgca gcttctgctg gtgtcatccg cggagctgga ccagatggcg
     1441 gcccaaagtt acgccaagct caaggccgaa gccagcgcca aaggcacgct caacaccaat
     1501 gcggccatgc tggcccgcgt gcgcgcagtg gccgcccgca tagaaccgca gacagcggtg
     1561 ttccgcgccg acgcaccgcg ctggcagtgg gaggtcaacc tgatcaccag cgaccagctc
     1621 aatgccttct gcatgcctgg cggaaaaatc atgttctaca ccggtctggt ttccagactc
     1681 aacctcagtg atgacgaaat tgctgtcgtg atgggccacg aaatcgccca tgccctgcgc
     1741 gagcactcgc gcgaacaggt gtcccaggca gcggccgcac agaccgccat cggcatcggc
     1801 acccagttgc tgggtctggg tggaggcacc tcacagatca cggaagccgc ctacgaggcc
     1861 ctggtggcta cacgtttcag ccgttcgcat gaaagcgaag ccgaccgcat cggcctggaa
     1921 ctgacggcac gcgccggcta caacccccag gccggcgtga gcctgtggca gaaaatggcc
     1981 agtgcaggca gcaccggcgg cggcgtcgat tttctgagca gccaccccac cgatagcagc
     2041 cgcgtgcagc agatccaggc cttgctgcca acggtgatgc cgctgtacca ggcgacgcgc
     2101 cgctaaactt gtcattccag acctgacgcg caagccccca cattgccgct gtcaggtcag
     2161 gcacggcgcg caggcgtgcg cctgcggcag caacaaaaac aatacgggga aagccatgcc
     2221 cgcagccatg accacgaccg agatctacct gatcgccatg gcgatcatct tcacggttcc
     2281 ctacctgatc tggcggatct tcaagaccga ctactacgcg ccgctggtgg tggtgcagat
     2341 cattgccggc atcctgctcg gtcccggcat cctgggcaag ctctaccccg actactacac
     2401 cttcgtgttc agcgcgcctg tcgtgcagtc gctcaacggc atcgcctggt gggcggtgat
     2461 ggtgtttgtc tggatcgccg gcatcgaact cgacctgaaa aaagcctggg cacaccggcg
     2521 tgagagcacc atcaccgccg gcctggcgct gggcaccccg ctgttgttcg gctgcgcggc
     2581 cgccgccggc atgctgatgt acgagggctg ggtcggcccg cagggcatgc gctggcagtt
     2641 tgtcatgggc gtgggcatgg cctgcgccgt caccgcgctg cccatcctga tcctgctgat
     2701 ggaaaagctt gccatcctgc gccaacccat cggccagcgc atcctgcgtt acgccagcct
     2761 ggacgacatc gcgatctggg gcgtgctggc gctgatcctg atggactggg agcgggtcgg
     2821 ccgccagggc agctttcttg cggcctttgc cttgctcgcc tatgtcttcc ggaagttgat
     2881 gctgtgggtg ccggaacgcg accgctggta tgccggactc gtctggctca tcctctgcgc
     2941 cctgggtgcc gactgggccg gcctgcactt catggtgggc gccttcctgg cgggcgccat
     3001 catggacggc gattggttca accaggaaca catggatatg ttgcgccacc atgtgctgct
     3061 ggtgatgatg ccggtttttt tcctgagcac cggcctgcgc accaactggt cgatgggtgg
     3121 cgaagccgta ttcatcgctg cggcggtact gctggtggct tcggtggccg gcaagctggt
     3181 cggcgtccat atcgccggca aggtactcaa gtgggcgcca ggcgagagtt ccatcatcgg
     3241 ctggctgctg cagaccaagg cgctgatcat gatcattttt gccaacatcc tgctggacaa
     3301 aggcatcatc accaacgcaa ccttcacggc gctgctgctg atggcgattg ccagcaccat
     3361 gttgacggtg ccggtggttg cgcccaggct tcagcgcatg aaagagatta ttttcagggc
     3421 gaagtgaaga cccgcagccg ctagatggcg acgagctgcc gcacgccatt ggcctgcatg
     3481 tccttgccct ggccgcgcgc gataaactcg ccgcgctcca tcaccaggta atcgtcggcc
     3541 agttcttccg caaaatcgta gtactgctcg accagcagga tggccatgtc gccgcggtcg
     3601 gccagcatgc ggatcacgcg gccgatgtcc ttgatgatgc tgggctggat accctcggtc
     3661 ggctcgtcca ggatcagcag cttgggcttg gccgccagtg cgcgcgcaat cgcgagctgc
     3721 tgctgctgcc cgcccgagag gtcgccgcca cgccggttca gcatctgctt caaaactgga
     3781 aacagctcgt acagctcggc cggaatcggc gtactcgcac ttttgtaggc caagcccatg
     3841 cgcaggtttt cctcgaccgt caggcgcgca aaaatctcgc gaccttgcgg cacaaaaccg
     3901 atgccggcgc gcgcgcgttc atacggcgtg gcgttctgga tggccttgcc gtcgaaatcg
     3961 atgctgccgg tcttgatagg caccagaccc atcagcgatt tgagcagggt ggtcttgccg
     4021 acgccgttgc ggccgagcag cacggtgacc ttgccgaggt gggcttgcag gctcacgtcg
     4081 cgcaggatgt gggagccgcc gtaatactgg ttgatgtttt tgacgtttag catttggtga
     4141 tctccatcgc gcttcttgga gcttcggagc catagtggtt cgccaacagg ccgggggtag
     4201 cccggcggct actcactttc ttttgcttcg ccaaaagaaa gtaagcaaag aaaaggcgcc
     4261 cctgctgtct gcgtccccct tcgcttaagc tacggggaca acctgcggtg ctcggtccag
     4321 ccggggccgg gctcgaactc gccttcggct cagacaatcg cccgtcctga tccgtctggc
     4381 cctgcgctcc tcggcgcagc cagaaggggg gtggggtcag gagcgggttc ggaatcggaa
     4441 tcgggtacgc agtcgggctc cggcgaggac gcgcaaagcg cgtcctcgcc tccccgcacc
     4501 cgtattcgct ccccaacccg tcgggccggg ccgaggagca cagccgaaag cggatcaggg
     4561 cgagcgattg tctgagcgta gc
//
LOCUS       DAIDFG010000254         4577 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_254_length_4577_cov_14.314905, whole genome shotgun sequence.
ACCESSION   DAIDFG010000254 DAIDFG010000000
VERSION     DAIDFG010000254.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_254_length_4577_cov_14.314905"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          871 a         1179 c         1344 g         1183 t
ORIGIN      
        1 cctattgcgt tctctcggac agcggaacaa tcactgaaga aacgtcgctc ctgaacctgt
       61 gctccatcac gcttcgaaat gcgcatgaac gacctgaagg gatggatgtg gggacgctga
      121 tcatgagcgg actccggaag gatcgtgtgc tggatgccat tgaaatcgtg aaatcacagt
      181 acagcaaaga ccggcgcccg gttttgccga ttgctgacta tgagaaccct catgtttcta
      241 cccagatcgt cagggttgtc acaagctaca tcgactacat caaccgggtg gtctggtcaa
      301 aaaaataata gactcgccgg tggtcggaac aagggtcttc ggcgtggagt ttttatgaag
      361 cgcttattcg atttgtgttc ggccctgctg gctggactgg ttttgctggt gcctattgcg
      421 ctggttgctg tgcttgtccg gttgacgtca aaagggccgg tgatttattg gtccgaccgg
      481 ttcgggcagc acaacacgat attcaggatg ccaaaattca gaactatgca agtggatacg
      541 cccgcagtgg caacgcatct cctggctgat ccaaagcagt tcctcactcc tcccgggtct
      601 ttcctgaggc aatcaagcct tgatgaactc ccgcaattgt ggagcatcct gaagggcgac
      661 atgagcctcg taggcccaag gccggccttg tttaatcagc atgatcttgt cgccttgcgc
      721 acgcaatttg gtgtgcatga attggtaccg gggctgacgg gttgggcgca gatcaatggt
      781 cgggacgaat tgcccattcc ggataaggtg aagctggatg tcgagtattt gcagcgtcag
      841 tcactgtggt ttgacatgcg tattctctgg cgcacttttg taaaagtgct gcgccgggac
      901 ggcgtgtccc attgaaagct acccgctaat gattaaaaat cctggcatcc gcttggggct
      961 ggtgtgtttg gttgccgcgt cggtcgggct gtctctagcc attgtaagta tcagtaaggt
     1021 cttgctggtg attgcggttc tggttgtgct tttgaggggc ggacggtgtg ctacggacgt
     1081 ggcgcctctg cagaagatgt ggaccccgcg gctggcgacc ttcgtggtat ttgtttttgc
     1141 attcagcctt ctttggacta ccgcacccat gaacagtgcg cttggcgcgg ttggcaagta
     1201 tggcaaattt ttggtgattc ccgctcttct ggtactgatt cgaacgcgcc aagaggcttt
     1261 gtatgccttg gcctttttcc tgggtgcgca agtttttttg cttctcagcg cctggttgct
     1321 ctattttcat gtcccggttc cgtgggccac cgcaaacatg gccaagacct cctacgcggt
     1381 cttttccagc tatctggatc agggcatcat gagcgcggtg gtttctgccg ttttctggca
     1441 tttgaaaacg ctcgctccta ctcgaccgtt gtttttcgcg gccatcgcta tatctatcct
     1501 ggcgctggga aacgtattga ttgttttttc gggccgtacg ggttatctcg ttgcagtaac
     1561 gatggtgtct ctggctattt tctgggctat gcctggaagg taccgcttcg cttccgtatt
     1621 cgtaccgcca ctgatagttc tggtggcctt tttcagtttt gacaaggtgg cgcagagatt
     1681 ttcggtcatc aagacggaga tatcggctta ttcttccaaa ccagatccgc tcagttcatc
     1741 cggcgtgaga ttaaatttct ggaaaacatc ggttagggcg attgccgaaa aacccttgat
     1801 gggatctggt gcgggtagtt gggtcacgga gttcaacaga attgagcaat taaaaaaccc
     1861 cgcacataaa agtctgggtt cttattacta ctatgcgcac ggcaaccctc accaggaatt
     1921 tctgctatgg ggtgtgcagt tgggagtggg tggaattctc ctcttgtgct cattttttgg
     1981 atccatcttg tttgacctac gcaagatgga tcaccctgtt gcccgggcag ggcagtcagt
     2041 gctgcttgca ctgatagtgt cttgcctgtt caactcatcc ctgtacgatg cctacattgg
     2101 tgattttttc tgtgtatcgc tgggcgtcct gttagcttac ggctttcata gtcgtgaggt
     2161 atcacggagc agcatcgcgg agggtgtgca acactttaaa tatgaagatc ggttgataaa
     2221 aacatgaaat ccgggaagcg aacctgggca tgggctcagg gtggggtacg tcgaaaacgc
     2281 ttttttctcc agcttttgtc ggacgatgcg catgcgtagc caggttctca gttggtcgcg
     2341 cgtgaccaaa tggttactgg tgatgttgct ggatgtcgtc atggccgtga ctgccatgtg
     2401 gatggcgttc tcgctgcggc tggacacgct gcactggccc tccgacatgc agtgggtggc
     2461 ctacgctttg gcacctgtgc ttgccgttcc ggttttcatc aaatttgggc tttaccgcgc
     2521 cattttcagg tacaccggcc tcgccgcttt ggtagcgact gcgcaggcgg ccgcagtgta
     2581 tggggtattg ctctttgcgc tgcttttgtg gcagcaatgg cctggcatac cgcgaactat
     2641 cggtttgttg cagccgctga tttttctggt ccttgtgggc atcagtcgcg cactggcacg
     2701 gttctggttg tcggaccccg gccgcatggc ccgccagata gaagggcgtc tgctgatcta
     2761 tggcgcgggc aatgctggcg ttcagacggc cactgccatc ggtatcaccg gtcagtacaa
     2821 gctgattggt ttcattgatg atgatgcgtc caagatgagg cgcagtatca atggggcccc
     2881 ggtatttggc ccagccgata tctcgcaaat cgtgacccga catcaggtca ctgacatctt
     2941 gctggcgctg cctcgtgcct ccagagagcg ccgcaatagc atcatcgaaa gtctgcgtgg
     3001 tttgcctgta catattcgta cgttacctgc ctgggccgac ctcgccagtg gacgggtgac
     3061 ggtgcgtgat tttcgtgagc tggatgtgga agacttgctg ggccgcacgc ccgtaccgcc
     3121 tgataccgac cggttagcgc gcgacctgac cggaaaggtg gttctggtta cgggcgcggg
     3181 aggcagtatc ggtagcgagt tgtgccggca gatagttcta cagcgaccgc gtcagttgct
     3241 actgctggat cacagcgagt ttggtctata cgccattcac caggagcttc aggccttgtg
     3301 ctcagccagg gagttttcag agcaatcacc agtggagctg gtcccgctgc tcgggagcgt
     3361 cctcaacttt gcacgcatgt ccgccctgtg ctcactttac cggcctgcca tcgtctacca
     3421 tgcagccgct tacaaacatg tgcccatggt ggagagtaat gccggtgaag gtattgcaaa
     3481 caatgtgttt ggcacgctta acatggcccg tgccgcgctg gagaacacgg ttgcacgttt
     3541 cgtacttatc tcgaccgata aagcggtgcg tcctaccaat gtcatgggcg ccagcaagcg
     3601 catggcagag ttggttctcc aagcgctggc ggaagagggt gcaccggtct tcctggatgg
     3661 cagccccgga tctggtagct ctcagggcaa ccagacctgc ttcaccatgg ttcgctttgg
     3721 caatgtgctt ggttctagcg gtagtgttgt gccactgttt cggcgtcaac tcgctgatgg
     3781 tggcccgctg acggtgactc atgccgaggt tacgcggtat ttcatgacca ttcccgaggc
     3841 tgctcagctg gtgcttcaag cgggcgccat ggcggtgggg ggagatgttt ttgtgctcga
     3901 catggggact cctgtgaaga tactggattt ggctcgccgc atggtggagc tatcaggcct
     3961 gacagtgcgg gaggacggtg cgactacggg tgatattgag atcaggatca caggtttgcg
     4021 gcctggtgaa aaactctacg aagaattgct gattggcgac aaccctgagc caacagcaca
     4081 tccccgcatc atgaaagccc atgaaccttg cctgtcatgg gctgacctta ccccggagtt
     4141 gcgtgctctc tggcttgccg ctcaggtaaa cgacgtcgat gcgatcaagg gcacgctcaa
     4201 acgccttgtg cagggatacc tgccgagttg atgcgtattc ggagattttg ttgtgcatgc
     4261 ggcacaattc aggaatttcc cttcttcttt attcaaggcc cgccgcaatg acgaccatct
     4321 cttccccgga ccccgacctc tcccacgtag gcccgcgcga caaggccgaa attctggcgc
     4381 aggcgctgcc ttacatccgc aagttccacg gcaagacgct ggtcatcaag tacggcggca
     4441 atgccatgac ggacccggcc ttgcaggcag actttgccga ggacgtggtg ctgctcaagc
     4501 tggtgggcat gaacccggtg gtggtgcatg gcggcgggcc gcagattgaa acggcactga
     4561 accgtctcgg caaaaag
//
LOCUS       DAIDFG010000255         4558 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_255_length_4558_cov_18.656451, whole genome shotgun sequence.
ACCESSION   DAIDFG010000255 DAIDFG010000000
VERSION     DAIDFG010000255.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_255_length_4558_cov_18.656451"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          836 a         1459 c         1452 g          811 t
ORIGIN      
        1 cccgaggggg ccgctgcgcc tgcgggccgg caaagccgga cccgcggccc cggctggtga
       61 agagtcgttt cgcccgcgct gcggcttgtg ctacattcca cccatgacct ccgcgaaatc
      121 ttattttgct tacttttggt tctcaagcgc ctccggcggt agagagatcc tcgcgtaagc
      181 agaaaaagac gcaacagatt cttgaaaacc gccggacctc cggcggtttt tttttgcccg
      241 tgctatttgc cgaattgatt gttgaacgtt ctaaaaggaa gccagccatg aatgccaaag
      301 ccaaccaagc cagcgatgcc tggtatgcga gtcctgtcga caaaaccagt cagaccgacg
      361 acgaacgcat caaggacatc actgtgttac ccccgcccga gcatttgatc cgcttcttcc
      421 cgatcagcgg cacccctgtg gagtcgctga tcacccagac acgcaagaac atccacaaca
      481 tcatggccgg caaggacgac cgcctgctgg tggtgatcgg cccgtgttcc atccacgatc
      541 cggcggcggc gctggactat gcgcgccgcc tggccgagca gcgcaggaaa tacgccggca
      601 cgctggagat cgtgatgcgt gtgtatttcg aaaaaccgcg caccacggta ggctggaagg
      661 gcctgatcaa cgacccttac ctggatgaaa cctaccgcat cgacgaaggc ctgcgcatcg
      721 cgcgccagct gctgatcgac atcaaccgcc tgggcctgcc ggccggcagc gagttcctgg
      781 acgtgatctc gccgcagtac atcggtgacc tgatcgcctg gggcgccatc ggcgcgcgca
      841 cgacggaaag ccaggtgcac cgcgaactgg cgtccggcct gtcggcgccc atcggcttca
      901 agaacggcac cgacggcaac atccgcatcg ccaccgacgc gatccaggcg gccgcgcgcg
      961 gccatcactt cctgtcggtg cacaagaacg gccaggtggc gatcgtgcag accagcggga
     1021 acaaggactg tcatgtgatc ctgcgcggcg gcaagacgcc caattacgat gcggccagcg
     1081 tgggtgcggc ctgcaaggag ctggaagcgg ccaaactgcc ggccacgctg atggtcgact
     1141 gcagccatgc caacagctcc aagcagcatg agaaacagct cgacgtggca cgcgacattg
     1201 cagtccaggt ggccgggggc tcgcaccagg tcttcggcct gatggtcgag agccacctgc
     1261 agccgggggc acagaaattc acgcccggca aggacgatgc caaaaacctg gaatacggca
     1321 aaagcatcac ggacgcctgc ctgagttggg aggattcgct caatttgctc gatgtgttgt
     1381 ccgacgccgt caaggcccga cgcgcgcgtt gacgccgcgt cctccaattg ggggatggcg
     1441 tgctaacaat ttggtgctaa atttggaact tctggttaca cttccacccc atgccacgac
     1501 agcggttgtc acatggaaga accggtcgtc acgaaagcac catgtcttcc gagcaacccc
     1561 ttctctttct gggtctggct ggtttcacgc ccgagcaacg cgagcggctc catctgctgc
     1621 tcggccagtt gccgcccggg ggcgcggtgt ggcgtgtcgg cacgtttgca gaggccgatg
     1681 cctggctggt tagcggggaa aaatgccgtc tggtggccgc cgacaccctg aaaattctgg
     1741 ccggcctgcc caccgagcgc accgtgcagc tcgatctgga cgaggtcgac cgcccgatcg
     1801 ctttctcgct gccgctgtcc tccgacgatt ttgatcccaa gcacaccttc gacctgggtg
     1861 tggctgccag cctgtccggg gtgctgcggc atttcgagag ctggttgcgg cctttgcgtt
     1921 cccagtttgt attgggcggg caactggtcg cgcgcgagaa cgagttgctg cccggtatct
     1981 accacgtcag ccacaggggc cagttgctgg ctgtgatgga ttttcatgag tggcgcatcg
     2041 gcattttgcg caaggccgac ccggttgatt tcgaactggc cctgtgggac cggcgccccc
     2101 tggaggcgca cgacatcccg agtcattttc tgcgcagcaa cttgactcat ttgcgctgga
     2161 tttacgcgca gcgcacggag cgcgacgtgt tgcctgcgcg ctatcgcagc aagccggttt
     2221 attttcgcca tgcgccggcc gtgcccatgc gctggatcag ggactcgcag ctgatcctgt
     2281 tgcgcgagtt gtcgcaggca gcaggggact ttgacgcctt gctgcggcgc accgggctga
     2341 cgcaggccca gctgacgcgg gatctggcca gcctgtattt cgccggctcg atcaccacga
     2401 cacccgacaa ggcgggagcc gccgccttca cggacgggga ggacagcctc cccggaaaaa
     2461 gccgcaacgg gcccgcacag gcgctgccgg gcgcacctgc ggggccgccg accctgcccg
     2521 tgcgcaagca tgacctgacg gcgccggcgc aactgggccg caaataaacc tgcgggtgcc
     2581 gccggctcgg gtttcagccg attgcctacc tgggcggcgg tgttcatgct ggtagacaat
     2641 cccgattcga taccaaggaa acccatgcac agcgaagtca gcgtcacttt caaaaacagc
     2701 ccgcccctgc ggatggacct caatgaggtt cagcccatgc cgcacgatgc ggccagggcg
     2761 tggctcgatg agcagttcac ccagatgggc tgcgagccgc tgcggcccac cggcaagttg
     2821 ctgatggccg acaaggtgct ggtggtggcc caggccgccg gcccgcagca gtttgccgat
     2881 gccgcctggg cccaggcctt tgcgcgtgca gccagcgcct cgctggccaa accggtgatt
     2941 cacatcgatg tgccggcgat ggcagtcagc tactgaactt gttgatgatt ttggcctgca
     3001 gcccttgaag ggaaagcgcg gacagctatc aaaaaggtag caaatcagcc agcctgcagc
     3061 gcctccagca gcctggcgtg aatgccgcca aatccgccgt tgctcatgca cagcaaatgg
     3121 tcgccgggac gggcggcggc cttgacttgc gccagcagct ggtcgaggtt gtcggccacc
     3181 tgcgcgcgct cgcccatggg cgccagcgcg gcccgggcgt cccagtccag tccgccggca
     3241 tggcaaaacg ccaggtcggc ctgctccagg ctccagggca gttgcgctgt catggtgccc
     3301 agcttcatgg tgttgctgcg cggctcgaag atggccagaa tgcgctgggc accgcccgtg
     3361 ctggccagct ggcggcgcag tccgtcgatg gtggtgtgga tggcggtcgg gtggtgcgca
     3421 aagtcgtcgt acacggtgat gttgccgccg gcgcgcgcca ccgtgccgcg tacctccatg
     3481 cgccgcctga cgttctcgaa actgcgcaag gcctcggcag cggcctgtgg cgccacaccc
     3541 acatggtcgg ccgcggcgat ggcggccagc gcattgagct ggttgtgcat gccaccgatg
     3601 ccccattcaa cacgcgcgac tttttggccg tctcgaaaca cgtcaaaagc cgcgggttcc
     3661 ccctcggccg agaagccttc cgccgcggag ccgccgaaaa gtacccgctc actccagcag
     3721 ccctgagcca gcacgcgggc caggctgtct tcgcgggcgt tgacaacaat cctgccggtg
     3781 ccgggcacgg tgcgcaccag gtgatggaac tgacgctcga tcgccgccag gttgtcgaag
     3841 atgtcggcgt ggtcgaactc cagattgttc aggatggcgg tgcgcggccg gtagtggacg
     3901 aacttgctgc gcttgtcaaa aaatgcggtg tcgtattcgt cggcctcgat cacaaaggtg
     3961 ttgccggtgc ccagccgggc cgacacgccg aagttcagcg gtacgccgcc gaccagaaag
     4021 cccggctgca ggccggcgca ctccagaatc caggccagca tggccgtggt ggtggtttta
     4081 ccgtgggtgc ccgcaacagc cagcacatgg cgcgggccgg tggggtggtg cgtcgggtgg
     4141 tgcaacacat ggccggacag ccactgtgga ccgctggtgt agggaacgcc ggcgtccagg
     4201 atggcttcca tcagggggta tctggcttgt ccgctggcgt catgcgcgcg ggacaccaca
     4261 ttgccaacca cgaacacatc gggcgcgaag gccatctggt cggcgccata accttcaatc
     4321 aattcaatcc ccagcgcgcg caattgatcg ctcatgggcg ggtagacgcc cgtgtcgcaa
     4381 cctgtgacct tgtgcccggc ctcgcgggcc agcgcggcca ggccgcccat gaaggtgccg
     4441 cagattccca gaatatgtat gtgcatgcag tattctagga ggcaagcggc tggccggaag
     4501 tttgtaatga aatacggctg cagcccaaac agatcgtgcg caggcagcta tttatttc
//
LOCUS       DAIDFG010000256         4552 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_256_length_4552_cov_16.191239, whole genome shotgun sequence.
ACCESSION   DAIDFG010000256 DAIDFG010000000
VERSION     DAIDFG010000256.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_256_length_4552_cov_16.191239"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          824 a         1512 c         1450 g          766 t
ORIGIN      
        1 ccggcgctgg agcagtacca cttcatcggc aaggacatcg tcaccttcca caccctgttc
       61 tggccggcca tgctgcattt cagcggccgc aagacgccgg acaacgtgtt tgtgcatggc
      121 ttcctgaccg tcaacaacgg cgagaagatg agcaagagcc gcggcacagg gctggacccg
      181 ctcaagtacc tgagcctggg catgaacccc gagtggctgc gttattacct ggccgccaag
      241 ctcaatgcca ggaacgagga catcgacttc aacgccgacg acttcatggc gcgggtcaac
      301 agcgacctgg tcggcaaata cgtcaatatc gccagccgcg cggccggatt cattgccaag
      361 cgcttcgccg gccggctggg ggaaatctcc gccgacggcg acgcgctgct gtccgtgctg
      421 cgcgcccaga gcgacaccat cacgcagctg tatgaattcc gcgaattcgc caaggccctg
      481 cgtgaagtca tgctgctggc cgaccgcgtc aacgcctatg tggaccagaa caagccctgg
      541 gaactggcca agcaggccgg catggacgga cggctgcatg atgtgtgcac ggtctgcatc
      601 gaggcgtttc gcctcttgag catttacctc aagccggtgt tgccggccct gggcgcggag
      661 gtggaaaact tcctgaagac cgggccactg ggcttcgccg atgccgctgc cggcctgggc
      721 aacggccaca tcatcggtga ctacaaacac ctgctacagc gtgtcgacat caaacagctc
      781 gaggccttgt ttgagccgcc agtccaggaa acacctgcgc catcagctat cgaatcaata
      841 gtacctggcg gcgaggagat tgcacccgtc atcaccatcg acgacttcag caaaatcgat
      901 ctgcgcatcg caaaaatcgt caactgcgaa gccgtcgaag gctcgaccaa gctgctgcgg
      961 ctgacgctgg acgtgggcga agcaagcctg cgcaacgtct tcagcggcat cgcttcaagc
     1021 taccaaccgc aagacctgat cggcaagcac acggtgatgg tggccaacct ggcgccgcgc
     1081 aagatgaagt tcggcatcag cgagggcatg gtgctggccg ccagccatgc caatgaaaaa
     1141 gccgagccgg gcatctttgt gctcgagccg ctgcccggcg cgcagcccgg catgcgcgtc
     1201 aggtaaagag gcgctgaatg agtccttgcg gatgagctga tcgcgccgca ggggacttgt
     1261 tcggcgcttc cttcaactca ggcgacaaat cgcagggcct tgcccgggcg ggctgccatc
     1321 tcggtgaccg cagcatcgag cgcctgcagc agcagtgcca cccccgccgc ctgaaacggc
     1381 gccgcagcaa tggcaacctt gagctgcagc accgcacccg gtggcagccg ttcgctgggc
     1441 gccagtcccc gcgcaatcag gcgctgcccc atttccgaga tctggtcgcg cgtgacacgg
     1501 cctttgagga tcagcacaaa atcgccgtca cggtggcgcg ccacggtgtc accctcgcgg
     1561 gccacctggg tgatgcaggc gcccgcatgg accagcgcca tctgcgccac ctgcaggccg
     1621 tactcgtcgc ggattgcctg gaggttgcca atgcgcacgc gcagcgccgc acccgacgcg
     1681 ggctcgcgca gctggtgaac cagcagctgg tgcagacgtt caagcatcac ccgatggttg
     1741 accgcaccgg tcagcgggtc gatccgggcc atggcgccga tgcgggcccg gttgtcgcgc
     1801 cgttcccggg tgcgcacgta caaggcaatc agcagcaggg ggatctccag cgccacgccc
     1861 agctgcgcgc catattgcgt catgaaagac acgggcacca gttgcatgtt gcgcaggacc
     1921 gggaacacgg cgccggccag caccgccagc acgccggcca gcacccacag gccgacccgc
     1981 ggaatacgcc aggcgtacca cagcgccaca ccgagataaa tcacgaaact ggccaggtag
     2041 taggggccgg agatcgcaaa aaacgggttg cgtccgacaa acaggaaacc cagcgccagc
     2101 atggcaccca cgcctgccat ggtcagcatc aggcgggtga cccagagggc gccgcgcccg
     2161 accaccagag cccgcatgaa gagatggccc aacgccgcgc ccgtcacggg cagcacattg
     2221 gacgccacgt cattccacca ggggttgtcc ggccagaaat actcgccggc caggcccgtg
     2281 agcgagagct ggcccaaggc gatcaccgcc acataggctg cgtaataaaa atgaatggag
     2341 tcgcgccaga aataggcgtt gacggcgcag atcaccagca ccagtgccac cagcccggca
     2401 taggcgccca gcaggaaatg ccagcgcttg ctggacgcct ggaaatcgcg cgcagaccac
     2461 accgtccagc gcacgctgat ggggtggctg tgctgtaccc gcaggtagct gggccgggct
     2521 tcccccggct gctgcaccag ctcgaacgcc ggatgcaggt aaggcaaggg ccactgcgac
     2581 accgggatcg aatcgcccga ccgcagggca tgccagctac cgtcggctgc gggccggtac
     2641 agatccacgc ggttcatgcc ggggtgcggc agcgccagca ccagcggggt cggctcactg
     2701 acggcgggca gtgccagctg gtaccacagg gcagcggtgc cgcccgacgg catgatgcgg
     2761 tctgcgcggg ccggtgtggc ctcgccgccc gcaaaacgct gctgaacctg cgccagcgtc
     2821 tgctgacccg ctgtatccac caggaccagc gcgtcgcctt gccggcccag ccatccggac
     2881 gccggggccg acccgctctg cgccagtgtc aacgaactgg ccgacagcca acatgccgcc
     2941 agtacaaagg caacaaaggc gaacagtgcc cggccgggac ggcccgtggc ccgacatgac
     3001 aaacaccaac ggcggaggca aagtggcatg gagcgtgcgt gagcggctta agatgagcaa
     3061 ccgcaggtat ttgaaataag aaaagacgtg tcccacttta gcaagggact cccggtctga
     3121 ccatcccaca gttggaggat gacccggccg atgtgtcatt ctggagtctt ctcatgaact
     3181 tgcccgccta cctcgcgccg tttcgccccc gcatcctgtc ggcgctgcgc ggctacacgc
     3241 gcgagcgttt cagcaaggac ctgggtgcgg gcatgaccgt cggcattgtg gccctgccgc
     3301 tggccatggc ctttgcgatt gcatcggggc tgtcgccgca ggccggcatc tggaccgcca
     3361 tcattgccgg cgggctgatc gccctgctgg gcggctcggc cgtgcagatt ggcgggccgg
     3421 cgggtgcctt catcgtgatc gtctacggca tcgtcgagca atacggcctg cccaacctcc
     3481 tgattgccac cgcctgcgcc ggcgtgctgc tgttcctgct cgggtttctc aagatgggca
     3541 ccctggtgcg ctacgttccg gtcagcattg tggtcggttt taccaacggc attgccgtgc
     3601 tgattgcgct gtcgcagctc aaggatctgc tgggcttgca gattgccaaa atgccggccg
     3661 actttttctc gcagatcaag gccctggcca caaacatcga ctccttcaac gccagtgccc
     3721 tgggtctggg tgtggcctgc tttctgggcc tggtgctctg gcccaaactg tttgtccccc
     3781 gccccggcgc tggcaatggc ctcgccgcgc gcctgatcaa gcgggtacag gtggtcgagg
     3841 gcctgcaact ggtaggtgcc acccgggtgg tgtcccgcgt gccgggaccg gtggtggcgc
     3901 tgatcacgct gacggccttt gcctggctgc tgggtctgcc ggtggaaacc ataggctcgc
     3961 gttttggcgg tatcccacgc tcgctgccgg cctttgcact tcccgatttt tcctgggata
     4021 cagtgcggct gctgctgccg cccacgctga ccatcgccgt gctgggcgcg gtggagtcgc
     4081 tgctgtgcgc acgtgtcgcc gaccagatgt ccggcctgcc caaacatgac cccaaccagg
     4141 aactgatggc ccagggcgtg gccaattttg tcgtgccctt tttcggcggc atgccggcca
     4201 ccggcacgat cgcccgcacc gtgaccaacc tgcgcgcggg cgcgacctcc ccggtcgccg
     4261 gcctggtgca ttcactcatg ttggtggggg tggtgctgat cgccgcgccg ctggccgtgc
     4321 atgtgccgct ggccgtgctg gcgggcctgt tgctgcatgt ggcctggaac atgggcgagt
     4381 ggcgcgagtt tgcgcacctg caccagtaca gcaaccacta ccggctgctg atgctgggca
     4441 ctttcttcct gaccgtggtg tttgacctca cggtcgcgct gcaggtcggt atgctgctgg
     4501 cctgcgtgct gtttgtgcag cgcatgagca gtctgttcca ggtggtggag ac
//
LOCUS       DAIDFG010000257         4544 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_257_length_4544_cov_19.594342, whole genome shotgun sequence.
ACCESSION   DAIDFG010000257 DAIDFG010000000
VERSION     DAIDFG010000257.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_257_length_4544_cov_19.594342"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          750 a         1587 c         1569 g          638 t
ORIGIN      
        1 gccgggtcgg ggttgcggtt ggccggcggg cggcacttga gcacattggt gatgaaaacg
       61 ctggtcgaga gaccggcccg cggcgcggca gcgtcatggc cgcgctgcag gccgatggcc
      121 agcagcatgt tgtcgagcag cttgccggcc tggccgacaa agggttcacc ggcgaggtct
      181 tcgttttcgc cgggcgcctc gccgacgatg agccagtcga cctgcggcgc ggcgccgtcg
      241 gcggccactg gcccgatgcc aaacaccgtg tttttgcggc tttcgcagag ggcgcaggcg
      301 cggcagccgg ccaccgcggc ctgcagggcg ggccagtcca tcagctccac gccggcagga
      361 cgcggctgca ggccggtggc cggccgcgcc acgggcactt gggccggcgg tcgcaccgcc
      421 ggtgcggcgg cgggtgcgag cgcacgtgcc ggttcggcct caacatcaaa tcggccttca
      481 gcccttttcg gaggggcgtc aacagctact gtattgatag cagcatccgc tgccaccggc
      541 ggtggcgatg gcggctgcca gaggcgcacg cccatttcac gcagcatggc gcgctggcgt
      601 ttgtccaggt ccaggctcac ggcagcactt tcatggctcg ctgtgtccgg cttgcagtgc
      661 catgctcatg accacggcgt cttcgcgggt ggcattcgcc gcggggtagt actggcgccg
      721 ggttccgacg tgacgaaagc cgtaacgctc gtacaccgcc ctggcccggc cgttgctcac
      781 gcgcacttcc agccacagcc actgcgcgcc ctggccgcgc gaccagagtg ccagcgcatc
      841 gagcatcacg cgggcccagc cctggcgctg gtgggcaggc gccactgtga tgttgagcag
      901 atgcacctcg tccacacctt tcatggccac aaaatagccc agcagctccg cgcccggccc
      961 gctgccgccg gtgagcacca gcgcctggta accggccttg agcgaatcgc tgaaattgcc
     1021 gcgcgtccac gggtgcgcat aagcactttg ctcggcccgc agcaccgcat cgaaccacga
     1081 ctcggtcatg ggttcgaact gggcttcgag gggttcgaga acggcgctca tggctcatta
     1141 tcggccggcc gccgcggcga tgccggcctt gacgcgggcg cgctcttcgg tggtcagcgc
     1201 caccttgtca cgcacgtaca gcggcagcgc ctggctggcc ggcacgccgt ggccggcggc
     1261 cagcagcgca ggcgccagcc gcagcatggc cacagccgtg ggcagggcgg tgaggcgcgg
     1321 caggctgtcc ggaaacaccg gcaggcgcgc cgcgtagacg ccgaacacat tgccggccag
     1381 caggtcgtcc gccggatcga gcggcaggtt ttccgggcgt atcagggcgc agtcggccag
     1441 cgaggtccag cgcccggcgt cgcaggcaaa acgctgcaca tacatctcgt ccatgcgtgc
     1501 atcgagcagc gccgtcacct gcaggcgggt cgcggcctgc ggaccttcgt gctggaagcg
     1561 ggcctcttcg gccaccgcca tcagtgtgtc cagcggcagc accggcaggc gcgcccccag
     1621 cgccaggccc tgcgccacgg agcacgccgt gcgcaggccg gtgaaggagc cggggccgcg
     1681 gccgaacacc agtgcgtcca gatcggccag cgccagcccg gcctggccca gcagttcgag
     1741 aatcgccgga atcagcgtgc tcgacgcctg cgcgccgccg gggccggtgt gctgccagat
     1801 gcgctgaccg tcggtgaggg ccagcgacat gctgtcggtg ctggtgtcaa aagccaggag
     1861 tttcatgggt tgatgagtca aatcaggcct tggcccttgt ggagtctgcg ctagcagcta
     1921 tcaaatcagg agtggttgtc gtgatgcgtt gggcctgcac acgcacgccg aacacaatgc
     1981 ccagggtcac cagcgccaag ccggcgagca ggttccagtg cagcggctcg ccgaggaaga
     2041 tcaccgcgcc cagcgccgac aggccgggca ccaccgcggt gatcatggtc gagcgcaccg
     2101 ggccgaagga ctgaaccatc ttggtgaagg tgatgccgga gatcaccacc gagcccacgc
     2161 cctggaacac catctggaaa acaatctcac tccagggcgc gctggccagc cgggtggcga
     2221 tgacgcccga atacgccagc aggctgtaga ccggcacata ggtcatgaag gcaaacacgc
     2281 tgatggcaat ggtggcagac accgcctgca gcgcatgttt gcgcaccagc accgaataaa
     2341 ccgcccagca ggtggaggcg cagagaaaca gcagatcgcc cttccagacc tggctgccgg
     2401 cctgcgtcca ggcctgcagc aggctggcgc cgcccaccat cacgtcgccc aggaaaatca
     2461 ggcccaggcc gatggcgcgt acccgcgtca cgcggtcttt caggatcagc acggccatca
     2521 gggtggtgct cagcggcagg gtgccgggca gcagcaccga ggcatgggcc gccggggcga
     2581 aataaaagcc gctgtaggcc aggcaggcgt aaccgacacc gccgaccagg ccgatcagcg
     2641 cggtgagccg gaaacccagt ggcgacaggc ccagccagct gggcggcgcc agagcggggc
     2701 ggctgtcgcg cgcgcggcgc cagaccagcc aggcacccca aggcagcagc accatcgccg
     2761 caccgatgaa acgcaacagg ctgatgtcca gcggcgtgag gctgcggtgc gccgtggcgc
     2821 gtgcaatcac gatgaaggag gtccagatgg cgacggtcac gatggcggca agcacaccga
     2881 ccgtcttggg agaaaaacgc atggcttgat tatccgggca agcggccgct tcgcagggca
     2941 aggccgataa tcagccatgc tcccctctcc cctgttgctc tgccggctgg ctgccgcgct
     3001 cgccctgggc ctggcccccg gctgggcgct ggcccagaaa gccgtgcgcg cccccaggct
     3061 ggcgctgccg ccggccatcg ccaaggccct ggtgcgtgcg cgggttccgc ccgacgccgt
     3121 cacgctgctg gtgatggatg cctcgggacg gcagccgccg cgcctggaac accgcagcac
     3181 ggtgcccatg aacccggcct cggtcatgaa actggtgacc acctacgccg cactcgacgt
     3241 gctcgggccc gactacacct ggaacacccg cctgacgatg gacggcagcc tgtcggaggg
     3301 actcctgcac ggcaatctgg tggtgcgcgg cggcggcgac cccaagctgg tggtcgaacg
     3361 cctgcaggca ctgctggggc aggtgcaggc cagcggcgtg aaggtgatac gcggcgacat
     3421 cgtgctggac cgcagcgcct tcctggtgcc ggcgctggac gccggcgagt tcgacggcga
     3481 accgctgcgg ccctacaacg cgcgccccga tgccttgctg atcaacttca agtcactggt
     3541 gatgcgcttc acgcccgacc tggccaatgg ccacgcacgg gtgcagaccg agccgcccat
     3601 ggccggcctg ctggtcgacg acacggtgcc gctggcacgt gtgagccgct gcggcgactg
     3661 gcgcagcgag ctgcgcgcca gcgtgcacag tcccgaccgc atccagttcg ccggcagcta
     3721 ctcgtcggcc tgcggcgaac gtctctggcc gacggcctat gccgagcccg ccacctttgc
     3781 ggcgcgcgcc atcgaaggct cgtggcgcgc gctgggcggg ctgctgaccg gcagcgcacg
     3841 cgacgccaca ccggccgagc tggccatgtt gcgcgcacgc ggcacgctgt ccggtgaaaa
     3901 agcgccgctg cgcctggacg cgccctcgct gccgctgcgc gacatcgtgc gcgacgtcaa
     3961 caagttctcc aacaacgtga tggcccagca cctcttcctc acgctggggc gcagcgccca
     4021 gccgggcgaa cacccccagg gcacgccgga aaccggccgt gccgcgctgg cagcctggtg
     4081 gaaaaaaatc ctgccgcggc aggaggcccc ggtgatggac aacggctcgg gcctgagccg
     4141 cgtcgaacgc atcagcgccg ccagcctggc ggccatgctg caggtcgccg cgcagggccg
     4201 cgtggcgcag gacctgctcg actccctgcc gatcgccggt gtggacgcca ccatgcgaac
     4261 acgcgccacc gcggtcaccg ggcaggcctt catgaaaacc ggctcgctgc gcgacgtgac
     4321 ggccgtggcc ggctatgcca acggcgcgag cggtgcgcgt tacatcgtgg tcggcatggt
     4381 caaccacccg aatgcgatcg cggcgcgacc ggcgctggat gcgctgattc agtgggcggt
     4441 gcaggaacgg taatgccgcg ttgggttgtt gcatcacgct gcggcttggg ttcgctatgg
     4501 acagtaggcg caactcccca ctcccacccc tagcccctgc cctc
//
LOCUS       DAIDFG010000258         4531 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_258_length_4531_cov_18.456211, whole genome shotgun sequence.
ACCESSION   DAIDFG010000258 DAIDFG010000000
VERSION     DAIDFG010000258.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_258_length_4531_cov_18.456211"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          870 a         1497 c         1415 g          749 t
ORIGIN      
        1 gacagctggg tcaggtcaat gtccagaccc aggcgtggcg aggacctcgg ctgcatcgcc
       61 agatcgggcc acagggtgtc accgcggctg ccttccgagc cggattcaag ttcgatgatt
      121 tcgcggccca ccgcatacag cagcagcagt tcgcgatact cttccagggc cagtacctct
      181 gcgccgtccc caggctcccg aaacagggcc tcttcaatga tcctgaagac ctgccgcgtc
      241 ggccaggcag cctggacacg caacaaaatg gcggcatagg cggcgccccc cgcactggtt
      301 gcgacgtaac ggtcaaagga cagaatccgg gtgttgaaga ccttgttgac atcgtcacgc
      361 aagtcttcgt aatcctgcac gttgccggcc tggtgataga gatccagcag gtccagataa
      421 accagcgcac tggttttgac gttgtcgacc aggtgcttgc gaagcagcgc gatggccttg
      481 gcctgctgcc ccaaagaact gaaaaattcc acttgctgct gaagatcaaa cagttccgga
      541 accttgacgg tacgtggcac gaaaggcacg ctgaccgaga agcggggccg gtcgcgcggc
      601 ggcagcgacg ggatggactc ggccgccggg tagcgggagg cgcgcctgag ttcgtcgaac
      661 agtgattcat tgacgtcaag ctcggcggcc cggcttgcag cggccagggt cacgcttctc
      721 gaaaggccgg attccgtaat gaaagtactg tgttcggagg aagatgtggc gcccgccgcc
      781 tgggcggtgg agcgctggta caacacgaaa agcaggccca tgaccgcgat ggaaaaaagt
      841 gcggccaggc catacaccac caccttgcca aatcgcgcgt tctcggcctg ctcgagctgg
      901 tgccggtttt cggccagcgc cgcctggttg cgcatcatgt ctgcacgcaa ttggcgaagc
      961 tcggcttcca gcgtctggac cttgttgctc tccagggtgc cggccgccgc tgacgccgcc
     1021 aggggggcgc ccgcagacag tggttgcgga tctgccgccg caagcgtggt ctgcagactg
     1081 gcccccaccg cgcgcggttt ggtcgccgca agcaccacat aacggcgtat ggttttttgc
     1141 acgcatccgg cacgcagatg cacggtgacc acgggtccct cgacgggtat cgcagagcgg
     1201 atatggacca accagtcctg ggcggcggcg gccttttccg gcgttatccg gacactggcc
     1261 ttgtcgagcg gcttgtccac gtagaaaaca tcggcttcaa tacacagcgc ctgcgggtcg
     1321 tcgccggcgt caagcatcag ctgcatggaa agatccagcg gctgccccag cacggctgag
     1381 cgggtgctgg gccccagtgt gactgccaaa gcgcccgccg acagacacag ccacaagccc
     1441 cctgtcataa gggcgcggtg ttggattgga cgcaaaaagg tgttcacttg gctgattctg
     1501 gcatgtccgg cacatacgga aggtgcagac agaggtccct gggacaaaaa gagacaaggc
     1561 cccgggccct gcaggccccg ggccgacccg tctgaccctg cagcccacct ccccgcacta
     1621 tcgtggataa acccgacgcg caagtctaac tcgcgctcca ggaccacgca aacgacgcga
     1681 accctgcgaa caccccctcc aacaggaggc cggacgcggc ccgggctgct ggataatact
     1741 tttccatgaa accactttat aaaactgttg gcctggttgg cacgggcgcc atgggccggg
     1801 gcatcgcaca aatcgccgct caggcgggca gcacagtcaa gctgttcgac gcccagccgg
     1861 gcgcagccga caaggcgcgc gacgcgctgg cggcgcaatg ggacaaactg gtggacaagg
     1921 gccgcctcga cggcgcgctg gccgccgccc acaaagcccg cctgctgtgc gccgacaccc
     1981 ttgacgatct ggccgattgc gaccttgtca tcgaggcggt tgtagaacgt ctggatgtga
     2041 agaagtcact gtttttgtca ctggaaagtg ttgtttccgg acaaaccatc ctggtgagca
     2101 acacctcctc gctgtccatc acggccatcg ccgccggact ccagcacccg gggcgttttg
     2161 ccggctacca ttttttcaac cctgtccccc tgatgaaagt ggtcgaggtg attgccggcc
     2221 tcaagacaga agctgccacc tgcagcgcgc tggcggacta tgcgcggcaa atgggccaca
     2281 cgccagtgca ggcccaggac acgccgggct tcatcgtcaa ccacgccgga agggcctacg
     2341 gcaccgaggc cctgcgcatt gtcagtgaag gcattgccga tttcgccacc atagaccgca
     2401 tcctcaggga ccaggccggc ttcaagctcg ggccgtttga actgatggac ctgaccgcgc
     2461 ttgatgtctc gcacccggtg atggagtcgg tgtaccacca gtattacgag gagccgcgct
     2521 accgccccag cgtgatcacc gcacaacgtc tggccggtgg cctgctgggc cgcaaaaccg
     2581 gcgaagggtt ttaccgctac accgacggcg ccgtgcaaac ccctgcggaa ccgcccgtgc
     2641 ccgtggtggc cgagatgccc ccggtctggg tgtcgccccg ggcagcgcgc cgatcggagc
     2701 tgctgcaact gctcaagaac ctcggcgcca gcatcgagac cggcgcatca ccttcgatga
     2761 atgcacttgt gctggtggcg ccgctgggct ttgacatcac caccgtggcc gtggtcgagc
     2821 ggctcgatcc cgcacgcacg gtgggcatcg acatgctgat cgacgacgca gcctgcaagc
     2881 gccgcgtgct ggcgaccaac cctgcaaccc gcagcgacat gcgtgacgcc gcgcacgcgc
     2941 tgtttgcgcg cgacggcaag gctgtcagcg tgattcgcga cagcggcggc tttgtcacgc
     3001 aacgtgtggt ggccgccatc gtcaacattg ccagcgacat ctgccagcag ggtatctgca
     3061 gccccaaaga cctggagacc gccgtcacgc tgggcctggg ctaccccatg ggcccactcg
     3121 ccatgggtga ccgttacggc cccaccaaca tccttgaagt gctgttcaac atgcagaccg
     3181 tgtacggcga tccgcgttac cgcccttccc cgtggctgcg ccgccgcggc gcgattggcc
     3241 tgagtctcac gcatcaggag tcctgacatg gcaggcgcac tcaaaagcac cagcgacggc
     3301 gccaccctgg tgctgaccct gagcaacccc gaattcaaga atgcactcgg ccctgaaatc
     3361 tatgccgcgg gcgtggaagc gctcaacgcc gccgagaaca accccgacat ccgcagcgtg
     3421 gtcatcaccg gcgaaggcgc caacttctgc gccggcggca acctgcatcg cctgcaggcc
     3481 aaccggcaac tgccgacgga ggtgcaggcg cagagcatca acggcctgca cagctggatc
     3541 gattcgatac gcaccttccc gaaaccggtg attgcagcgg tcgagggcgc tgcggcgggt
     3601 gccggttttt cactggcact ggcctgcgac ttcgtggtcg cggcagacaa cgccgtcttt
     3661 gtgatggcct acagcagcgt ggcgctgtcg cccgacggcg gcggcagctg ggccttgagc
     3721 cgcgccctgc cgcgcgcgct ggtcgccgaa ctgctgatgt gcggcgagcg gataggcgca
     3781 ccgcggctgc aggcgctggg cctggtcaac cgcagcgtcg ctgccggtga tgcgctcaac
     3841 gaggcactga aactggccga cacgctcaat gcccgcgcgc ccaacgcgct ggccagcatc
     3901 aaggaactcc tcaatgacgc acaggccaac accctgagcc agcagctgga cgccgaacgc
     3961 gggcatttcg tgcgcaacct gcaccacccc aatggcggcg aaggcattgc cgccttcctg
     4021 gaaaagcggc cgccggccta cgactgagca ggccccgggc gcgcaggccg cgctggtgca
     4081 gggggcgggt ggccgggaca ccgaggcgac agatggatgt ccgcggcatc gtcgctggcg
     4141 tgacaatagc tctcttgtga gtcacccaaa agacaggcta tggacgatcc cattcttacg
     4201 atagacgaaa gagaggccat caacggtggc cgctggtttt catccctatc tccctcactc
     4261 aggcacgaca tacttcgatg cgcatacgtc caacgtttca aggatggcga actgatcgcc
     4321 gcccgcggcg acccgccgga acaatggatt gcctgcgcca aaggcgcggt tcgcgtgagt
     4381 tcgacctcga tctcgggcaa gcagatcacg ctgacctatg tggagcccgg catctggttt
     4441 ggcgatgtgg ccatcttcga cggcgaccgg cgcacgcacg acgcctatgc gcacggcgag
     4501 accaccatcc tgtgtgtggc caaggccgac t
//
LOCUS       DAIDFG010000259         4502 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_259_length_4502_cov_12.788397, whole genome shotgun sequence.
ACCESSION   DAIDFG010000259 DAIDFG010000000
VERSION     DAIDFG010000259.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_259_length_4502_cov_12.788397"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          857 a         1348 c         1333 g          964 t
ORIGIN      
        1 gccgaagatg gcgaccagca cgttggcgcc tgtgacttcc ttcttgccgt tgccggtgga
       61 ctgcacatgc atgatggcgc gctggatcac gcgctggaaa cccagcgtgg gctgcgtgtc
      121 cacgtcgtcg ctgccggcaa cctgcggcgt gttgtccttg atgaagttgg ccagcgactt
      181 gcgcaggtca tcgatattgg cggagcaggc gcgcagcacc tcggcggcgc tggggttgtc
      241 gagcaaggca agcagcaggt gctccacggt gatgaactcg tggcgctgct ggcgggcctc
      301 gacaaaggcc atgtgcaagc tgacttctag ttcctgggca atcattgctt ttcctttcgg
      361 cttgctgctg ggatatctga tgggatcatt gtgtaactct tcaagccgat atgggctgtt
      421 ttttcaatta ttcaatgggc tcgcacacac actgcagcgg atggccattc tttcgcgccg
      481 cttccgtgac ctggtcgacc ttggtggacg cgacatcctt ggaaaacacg ccgcacacgc
      541 ctttgccgtc caggtggatc ttcagcatga tctgcgtggc cgtttcccgg tctttgttga
      601 aaaattcctg gatcaccacg acgacaaact ccatcggggt gtagtcgtcg ttgagcagca
      661 cgacctggta catctgcggc ggctcggtct tttgcggacg gcgctcaagc acgaccgcat
      721 ccccgccatt ggcatcctcg ggcttgaagg ccggtgcctt gggtggtgcg ggagttttgg
      781 gaggtttgct ggccatggaa atcattctag cgaccggaat tgtccggtgc gcctgacagg
      841 gaaattggtg gcgggaacgc gacaatcaag cgcgcgcaat tgactttcga cctggcggtt
      901 caaaagccca tgaaaaaagc cgcccgacct gcgccgtgac ggctttgcag catattctgc
      961 ctggtttccc ggccacgcag cgggcggccc ggcagcgtgc tttcttacat gtggtcgatc
     1021 atgacctggc cgaagccgga gcaactgacc tgggttgcgc catccatcag gcgggcgaag
     1081 tcgtaggtga ctttcttgga ctcgatcgac ttttccatcg aactgatgat caggtcagcg
     1141 gcctccgtcc agcccatgtg acgcagcatc atttcggccg acaggatttc ggaaccgggg
     1201 ttcacatagt ccttgccggc gtatttcggc gccgtgccgt gggtggcctc gaacatcgcg
     1261 atggtgtcgc tgaggttggc gccgggtgca atgccgatgc cacccacctg cgcggcgagc
     1321 gcgtcggaga catagtcgcc gttgaggttc agcgtggcga tcaccgagta ttcggccgga
     1381 cgcagcagga tctgctgcag gaaggcgtcg gcgatcgaat ccttgacggt gatttccttg
     1441 ccggttttcg gattcttgaa cttcatccac gggccgccgt cgatctcgac cgcgccgaac
     1501 tccttcgcgg ccagcgcgta ggcccagtca cggaagcccc cttcggtgaa cttcatgatg
     1561 ttgcccttgt ggacaatcgt caggctgggc ttgtcgttgt cgatggtgta ctgcagggcc
     1621 ttgcgcacca ggcgctcggt gccttcacgc gaaaccggct tgatcccgat gcccgaggtg
     1681 ttgggaaagc ggattttctt gacgcccatc tcgtcctgca ggaacttgat gagttttttg
     1741 gccttgtcgg actcggcctc gaactcgatg ccggcgtaga tgtcttccga gttctcgcgg
     1801 aagatcacca tgtgggtctt ttcgggctcc ttcaccggcg agggtacgcc cttgaagtac
     1861 tggatgggac gcaggcagac atacaggtcc agttcctggc gcaacgcgac gttgagcgag
     1921 cggatgccgc caccgaccgg cgtggtcagc gggcccttga tggagaccac atagtccttg
     1981 accgcagcca gggtttcttc cggcagccag acgtcgggac cgtagatcct ggttgacttt
     2041 tcaccggcat aaacctccat ccagtgaatt tttttcttgc cgccgtaggc cttggccacg
     2101 gccgcatcga ccaccttgag catgaccggc gtgatgtccg cacccgtacc gtcaccttca
     2161 atgaacggaa tgatcggctc atccgggaca ttcaatgaat tgtcggcatt gacggtgatt
     2221 ttctgcccct gggcgggcac cttgatgtgc tgatacatgt gaagaagtct ccggtgcgcg
     2281 cttgacatca cagtgaatga ctgcccggcg ctgtttaggt gataacccgc aaattttagc
     2341 cccaaaactt cccacgacac atttgagcgc ctgcatgtca accgtttgca aacagggctc
     2401 gttacagtca tgtccatgag tttttcgggg gacctggttc aaaaacttat taggaaaact
     2461 ccaaatgaac aagattcttg ctgccctgat cgctggcctt ttcgctgctg gtgcttttgc
     2521 ccaagctgcc gcacctgccg cgcccgcaac accggccacg gctgccgcac cggctgcacc
     2581 cgcaaccgct gcaacgccag ccgcacccgc tgccaaggta gatgccaagg ccgccaaggc
     2641 cgaaaaggcc gccgctgcga aagccgccaa agctgaaaaa gcagctgccg ccaaagccgc
     2701 caaagccgaa aaagccgccg ctgcaaaaaa ggccaaggaa gaaaaagcag ctgccgccaa
     2761 agctgccaaa gctgaaaaag ccgccgctgc caaaaaagcc aaggaagaaa aggctgctgc
     2821 agcgaaagcc gccaaagctg aaaaagccgc cgctgccaag aagtaattct tcgcgcgcag
     2881 ctgaaatgcc cgctatgcgg gcatttttca tggctgtcgt ttatgctgag agcctgacct
     2941 ctttccggtg aaacacctta tgcccttcct gtcctgttcc ctcaagtcac ccatgcgcgg
     3001 gctggcgctc gcactttcct gcatggtgtt ggccaccgga tgtgcgctgg cgcaaggaac
     3061 gccacaaaca aacctgccgc gggtcaaact gtcggcagga atgcaccaga tcgacgcgca
     3121 actggccttg acccccgagc agcggcaaat cgggctgatg tttcgccagg aaatgccgtc
     3181 aaccgaagga atgctgtttg tgttcgaaca gcctgcccag cagtgcttct ggatgaaaaa
     3241 caccctgctg ccgctgaccg ctgcgtttgt cgccgactac ggcaccatcg tgaacctcgc
     3301 tgacatgaaa ccgcagacga cagactcgca ctgctcggaa aaacctgtgc gttatgtgct
     3361 ggaaatgaac gtcggctggt ttgccaaaaa aggcatcaag gccggcagca aactggcggg
     3421 accggtgttc gcgccgtccc gctgaacagg catgcgccgc gccattggcg gcaccatgaa
     3481 aaaagccgac ctgaagtcgg cttttttgtg ggccggtcaa ggcgtcaagg ttgtttacgc
     3541 cttgaaagac gccagtgccg cgttcagggt cttgctcggc cgcatcacgg catcgagctt
     3601 gtcgaagtca ggcttgtagt aaccgccaat atccgccggc gtgccttgca cggccgacag
     3661 ttcagccacg atggcctgct ccttctcggc cagttgcctg gccagcggtg caaacttggc
     3721 cgccagtgcc gcatcttccg tttgtgcagc cagttcctgg gcccagtaca tggccaggta
     3781 aaactggctg ccgcggttgt ccagctggcc ggtcttcggc gacggatttt tgttgttgtc
     3841 gagaagcttg ccagtggcgg catccagcgt tttggccaga atcttcgcct ggtcgttgcc
     3901 agtctttagc cccaaatctt ccagcgacac cgccagggcc aggaattcgc ccagtgagtc
     3961 ccagcgcagg tggttttctt ccaccagttg ctgaacatgc ttgggtgcgg agccgccggc
     4021 gccggtttca tacatgccgc cgccggccat cagaggaacg atggacaaca tcttggcgct
     4081 ggtgcccagc tccatgatgg ggaacaggtc ggtcagatag tcacgcagga tgtttccggt
     4141 ggcgctgatg gtgtccaggc cacgcaccac gcgctccagc gtgtagcgca tggcacgcac
     4201 ctggctcatg atctgaatgt ccagacccgc cgtgtcgtgt tcgtgcaggt acatcttgac
     4261 cttggtgatc atctgcgctt cgtgcggtcg gtaggaatcg agccagaaca ccaccggcat
     4321 gccggagttg cgcgcgcggg tcaccgccag cttgacccag tcgcggatcg cggcgtcctt
     4381 gacctggcac atgcgccaga tgtcgccggc ttccacattc tggctcagca gcacctctcc
     4441 ggtggacagg tcggtgatgt tcgccacgcc gtcttcggcg atctcgaagg tcttgtcgtg
     4501 cg
//
LOCUS       DAIDFG010000260         4476 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_260_length_4476_cov_19.823117, whole genome shotgun sequence.
ACCESSION   DAIDFG010000260 DAIDFG010000000
VERSION     DAIDFG010000260.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_260_length_4476_cov_19.823117"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          788 a         1464 c         1461 g          763 t
ORIGIN      
        1 gtgggccact cgctgatcat catcgaacac aacctcgacg tgatccgttc ggccgactgg
       61 ctgatcgacc tgggccccga aggtggcgaa gccggcggcc tgctggtggc ccagggcaca
      121 cccgaagagg tgcgccagca cccgacttcg cacaccgcgc tggcgctgcg ggaatacgaa
      181 caggccatgg gcaaggtgca tggtgtcgag gaagcgcggc ccaaggcctg gagcaccagg
      241 ccggcgttct ccaacctgat ccagatcgtc aatgccaagg agcacaacct caagagcctg
      301 tcggtcaaca ttccgcgcgg ccagttcaat gtggtgaccg gtgtgtcggg ctccggcaag
      361 tccacgctgg cgtttgacat cctgttcaac gagggccagc ggcgttacct ggagtcgctc
      421 aacgcctatg cacgcagcat cgtgcagccg gcgggccggc ccgaggtcga tgcggtttat
      481 ggcattccgc cgacggtggc gatcgagcag cgcctctcgc gcggcgggcg caagtccacc
      541 gtcggcacga ccaccgaggt ctggcacttc ctgcgcctgc tctacgtcaa gctcggcatc
      601 cagcactgca ccaaagacgg cgcggccgtc atgccgcaga gtgctgaaag cattgccgcg
      661 caactgctca aggcgcaccg cggacagcac atcgggctgc tggcgccgct ggtgatcaac
      721 cgcaaaggcg tttacacgga actggccgac tgggcgcggc cgcgcggcta cacgcatttg
      781 cgcgtggacg gtgaatttct gccgaccacc ggttttccgc gcatcgaccg cttcaaggaa
      841 cacacggtgg agttgccgat tgccgacatc cacgtgaccc ccgccaacga ggccgcgctg
      901 cgcctggcgc tggccaccgc gctggagcac ggcaagggtg tggtgcatgt gctggcgccg
      961 ctggatggcc tgcacggtgc gatgctggcc ggcacgtcca cccggcagat cggcgctttg
     1021 caggcctcgt ccaccaagcg ggcctgtccg gtctgcgcca cctcctaccc cgaactcgac
     1081 ccgcgtctgt tcagctacaa cagcaaacac ggctggtgcc cggactgcgt gggcaccggc
     1141 gtcaggctca ccagggagca gcgcaaggtg ttcgacgact cggtgcgcga cgatgacaac
     1201 aagggccgcg agcagagttt tgtcgaagcc gatgtggacg aggtgctgga cgccgtctgc
     1261 ccgacctgcg caggctcccg cctgaacgcg caggcgcgcg ccgtgaagtt cgcgggggtg
     1321 ggcattgccg acatcgctgc gctgtcggtg accgacgtgc gtcgctgggt ggaaaaactc
     1381 gcgctgtccg gacgcgaggc cggcattgcg cgtgacctga tccccgaaat caagagccgg
     1441 ctcgaattcc tcgaagacgt gggcctgggt tatttgacgc tggaccgtgg cgcgcccacg
     1501 ctgtccggcg gcgaggcgca gcgcatccgg ctcgccgcgc aactcggttc caacctgcag
     1561 ggtgtctgtt acgtgctgga cgagccgacc atcggcctgc atccgcgcga caaccagatc
     1621 ctgctgcgcg ctttaggcaa actcagcgaa cgcggcaaca cgctggtggt ggtggaacat
     1681 gacgaagaca cgattcgccg cgccgacaac atcatcgaca tcgggcctgg cgccggcaaa
     1741 cgcggcggcc agctggtggg gcagggcacg gccgaacacc tggccaccct gccggattca
     1801 ctgaccgggc gctacctggc caacccgctg attcatccgc tgcagattcg tcgcgaaacg
     1861 cagttgcccc tcacccccac cctctccccg caggggcgag ggagcaatgc cgggagttca
     1921 ctccctctcc ccccgggaga gggcgggggt gagggcgcgt tgcccaaacc actcagcaag
     1981 gccaaagcgg ccgcgctcaa ggccgcccgc ctgaaagcag aggcggccag cgccagcgtt
     2041 gcccacaacg ccgcgcccgc cgccgtcaac ccggtgacct cctggctggg cgtggtcaac
     2101 gccgacctgc acaacctcaa gaacgtctcg gttcaggtcc cgctgtaccg gctggtggcg
     2161 gtgacgggcg tgtcggggtc gggcaaatcc accctggcgc gcgacgtgct gctggccaat
     2221 gtgcaggccg gcgtggcgca acgttcgacg cgggcagggc gtgaagcgga cgatgccggc
     2281 aaacacccgg cctggaccgg ctgcgaatac atcgagggtt acgaagtggt ggaccgtgtg
     2341 ctggaagtcg accagacgcc catcggcaag acaccgcgct cctgcccggc cacctacatt
     2401 ggtttctggg acacggtgcg caagctgttc gccgacacgc tggaagccag ggcgcgcggt
     2461 tacggcgccg ggcgtttcag tttcaacacc ggcgaaggcc gctgcccgac ctgcgagggt
     2521 gccggtgtgc gcaccatcgg catgagtttt ctgcccgacg tgaaggtgct ctgcgagacc
     2581 tgtcacggcg cgcgtttcaa ccccgaaacc caggcggtga cctggcgcgg caaaaatatc
     2641 ggcgacgtgt tgaccatgga agtcgacgag gccgtcggct tttttgcctc catgcccaac
     2701 atcgcgcacc cgctgcagtt attgaaagac gtggggctgg gttacctgac gctgggccag
     2761 ccctcaccga cgctgtcggg cggcgaggcg cagcgcatca agttggtgac cgagctgtcc
     2821 cgggtgcgcg acgacatcac gcggcgcggc cagaaggcgc cgcacaccct gtatgtgctg
     2881 gacgagccga ccgtaggcct gcacatggcc gacgtggaaa aactcatcca tgtgctgcac
     2941 cgcctggtca acggcggcca cagcgtggtg gtgatcgagc acgacctcga cgtgatcgcc
     3001 gaagccgact gggtgatcga cctgggcccg gacggcggca acgccggcgg ccaggtggtc
     3061 gcggccacga cgccggagca ggtggtcaag ctgggtacgg ccaccggcaa ggccttgcag
     3121 ggtgtgctgg aaagacattg aacacccctg tcggcgctca ctgcgtgtag cgcctctcct
     3181 cttgcagggg acgacgcctg caggccggcg gagccgggcc tgcggcccct gctggcaggg
     3241 tcagagatac ggtattgcgc cattgctatt gaatttatag cttttggcgc acgatccata
     3301 agggctacgg ccttattttg tctgtcatgt cagccgggat cacgcgccgc cagcagcgcc
     3361 gccgccactt cggcaaagcc tgcgccgcgc tccccctcgg tgatgtaacg cggcttgtgg
     3421 ctcaactccg cttcaaagcg gcggatgttg gccacgccga ccgaatgcgc gaaggcctcg
     3481 aacatcagct ggtcgttggt cgagtcgccg acatagaccc agcggtccat ttcgctgtcc
     3541 aggtcgcggc cgaacaattc acggacgatc cagcgtgcac cctccagctt gttgtgtgcg
     3601 ccgaaccagc cgttgatgtg gatggagctc actgtcgcgt tcatgccctc gctttgcatg
     3661 atctgcacgg catgggcgat gctttccggt ggcaggtgca caaactcgct gtggtcgatg
     3721 gcgatgtcgg tttcgcgtcc tgggctgtct tcggacaaca cggcgccggg cacgtcctgc
     3781 atcacacgcc gggccacctg ttgcatgcgg gcgtagttgg ccaggcgtgt cggtgcgtcc
     3841 tgctgataca gttttgatag cggctcacgc ccgtcagtcg agggctgatg cccaatctgg
     3901 cttgcaagca gggccacggc accgttttcg gcaacgatgg catccaccgg ccagcgcagc
     3961 gcaaaaggct cgctccagcc gaccggccgc ccggtgatgg gaatcacatg caggccggcg
     4021 gccttcaggt cgccaagggc ctgcagcgca tcgggcgtga tcgcgccgtc ggtggtcagg
     4081 gtgtcgtcga tgtcggtaaa aacgccggtg atgtgttgcc ggtcgtgtgc tggccaggtt
     4141 ttcagggctt tcatgacgaa agtgtcgcat gcctgtacaa tcgtcgattc ctgaggagcg
     4201 ttgcaaccca caacatgtgg tctaggctca ggactttcat ttgcaaccac gctcacccgc
     4261 atgtgctttc aagccgggtg agatccaacc gactccccgc cgacttgtcg ccttgtggcc
     4321 gtggctattt tgcccaattg gacccttgat cttggagata ccccaatgaa cgccgtattg
     4381 aaacccgccc agaccgccac tgactacgtg attgccgaca tttcgcttgc cgcctggggc
     4441 cgcaaggaac tgaaaatcgc agaaaccgaa atgccc
//
LOCUS       DAIDFG010000261         4448 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_261_length_4448_cov_14.669702, whole genome shotgun sequence.
ACCESSION   DAIDFG010000261 DAIDFG010000000
VERSION     DAIDFG010000261.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_261_length_4448_cov_14.669702"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          891 a         1313 c         1331 g          913 t
ORIGIN      
        1 aggtaatttt tgtgcccctg aagcaagttc tgtcccatgg attcatgggt cttcttgagc
       61 acttcggggt ttgtgagcgg ccagttcgaa ggcgacagtg cgtcgagcca ttggcggctg
      121 aaaaaattca cgacatgctg gtgatgcctg gccacgccgt tcaccctggc ggattcctgc
      181 caccaggagt cactggcctt gaagttctct ttgagcgcct tgaagggcca ttttttccac
      241 gcgtcatcat tgaagcggtg gtcattttcc tggacaggtg tgccatccac ttcgacgtca
      301 gtttcgactt gcagggtatt gacccaggct tgccaggaaa gcgacatgcc gagacttccc
      361 aagagcgctt gccggctcgg agagactgcc aggtgcatgg cccagtccgc aaatgccaaa
      421 cttagcgaga tgggtgacag gcccatgctg gccttggaaa gctgcagatg tagcgccctg
      481 tcaatcgatg tcgcaaccgg gtgtggcggt ttgaatgtca gcgcaggagg gatgaatttc
      541 attgcgtctc gtgcaaaggg attgaaggat caggcaacgg cgtggcagcc ggcatcgacg
      601 taaatcgtct ggccggtcat gccggaagaa ccatcaccgc acaggaaggt gcagaggcgg
      661 gcaatttcct ccagcgtgac aagtcgtccc aaaggggcct tgcgctgggc atgcgccatc
      721 aattcatcga aagacgcgat gcctgaggcg gcacgggtga gaatcggtcc aggcgaaacc
      781 gcatgcaccc gaattcccat ggagcccagg tccacagcca tgtagcgaac cagggattcc
      841 agggctgcct tgaccggccc catcagaccg tagtggggca ctgcttctgt cgcccccagg
      901 tagctcatgg tgacgaagct gccgccgtcg cgcatatgag gtacacagag cttggcgagt
      961 tcagcaaagg agtggcaaga cacatccatc gcccggccaa aacccgaccc ggatgtttcg
     1021 atcacggggc tttgaaggtc cgccagggga gcccaggcaa tcgaatgcac cacgaagtca
     1081 aggccgccca ggtccttgac ggcacgttcg accagtgcct ggagttcacc gggcttttcc
     1141 acgtcacacg catagagggg aacaccgagg gagactgcca gaggctccac gaagcggaag
     1201 gctttttcgt tcaggcagga tgtcaccagg tccgccccct gcaagatggc atcgcgggcg
     1261 catccgtaag caatgctgtg ctcgttggcc aaccccaaaa tcaggccctt cttgtcagcc
     1321 agggacggcg gtgcgatggt ggcactcatg aagcgttatg tttgtggtga cgggtgggcg
     1381 tctgcgccgt tcgaagcgct cgaagtcgct cgatcagggc ggcatcggac accaggactg
     1441 cggccaccac gccggcataa agccttccat cgttgccgtc cagagtgatg acatcaccct
     1501 ccttgaacac ctgatcaccc aggcggacgg tgcgttcctt caggtcgatt cgcagggttt
     1561 cgcaaccgac caggcaaacc tttcccagct gtcgtgccac gacagcggcg tgagaggtac
     1621 gagcgccccg ctgcgtcagc aagccggcag caaattccag cgcggcgatg tcactggtct
     1681 cggcgtcctg gcgcactaga accaggctgg caccttcagc agcccgtgcc gctgcgcgtg
     1741 cgccgtccag aataatttca ccagacacca caccggagca ggccgacatg gcctgcccca
     1801 gcgggctggc ctgcgcttcc gggttgcctt gcgaagccag ttgaaccgtg ccaagggttt
     1861 cctcgtgcaa atctctcgtg cgctccaggg cggtagcggt gtctatcagg ccctcatcca
     1921 ggagatcaag ggctatgcgg gctactgcca ggggcgttcg cttgccagac cgtgtttgta
     1981 gcatgtgcag aacgccgtct tgtaccgtga attcgaaatc ctgcatgtcc gagaactctc
     2041 gctccagggt ctgcgcagca tcctgcagct tgcgccaggc atcgggcgcc acgctggcca
     2101 gagcctcgtg tccatgggca tttctccgcc ccgacacaac gtcctcgcct tgtgcatttg
     2161 cgagaaaatc cacccacggt tgacgctcgc cggtcgtggg gttgcgcgtg aagcccacgc
     2221 cggcgccgga atggcctccg ctattgccaa aaaccatgct ttgaatcgtg acggcggttc
     2281 ccatggcgtg gtcgatcagg ttgatctcgc ggtaggcgtg agccttttcc gcatgccacg
     2341 acatgaaaac tccacgaatg gccccggaca gttgttcatt gacatcctgg gggaacgggt
     2401 gacctgcgtg caactggtaa agcgtaagaa actgacgcgt caggctgcgc aattcagcga
     2461 agttcatctg ccgctcgtcg cggccttccg tgacgcgctc gatctcgtct tcaaacagag
     2521 aacccggcag gcctgccact acttcgccgt agctggcaat gaggcgccga taggcatccc
     2581 agaccagtct gggattgccg gtctgacgca gcaagccggg cagggtctga tcgcataacc
     2641 caatattgag cagggtctcc atcatgcccg gcatggacac gggtgcaccg gaacggattg
     2701 agacgatcag gggattgcgc aaatcaccaa gaacccgtcc ggccgcctct tgcaaggccg
     2761 gcagcccgat ggtgaacacg ggcagcatgc aatcttcggg tgattttgca tagtgtgttc
     2821 cgatgaccag cgctggcgga acgggaagtc ccatgcttgt catacgcaga aggttgtagg
     2881 ccttggaccc catgctttcc ctgcttggct tgtgctgggg ctgcgtgttg ccttcggcaa
     2941 tcagatacaa atgtttgggc cagaggctta ttgaactcat gcagacatac ctgttttggt
     3001 gaggaccatc ttgcgcagcg cataaccggc tgcgagaagg gcgtcggatg ctttttcgat
     3061 cgtggcggcc aagtcgcttg ccaggagcat gccggcaggc gaagcgtgaa gcgagacgac
     3121 aatgctcttg cgggcttggc gcgacaattc gtcgcagagc cgctccgcgc gcaacatgcg
     3181 ccagagggtc tgtaaaaaca tgtcgctatc ggcagcgtcg ccgtgctcac tgatctgccg
     3241 tgcgatctcg atgaccttga cctgatcctg aatggcggtc agcgtcgtat ccgccagccg
     3301 gcgcagcacc tcagtgaccg gtgccggaag ccccctcagg ggttctccca gtgtcatgga
     3361 gtggatgaag aaggcttctt caagggcatc ggccacatca tccgattttt caagaagttc
     3421 caccactggg cgccaccggc tttgtctctc tgcgcgctga cgtgcgtcca tcagcagatg
     3481 gtcggcctgt cgctcccatg tttttgcccg caaaacaagg ttggagtttt gagtgtcgtc
     3541 tatggccgtc ccggtttcga gagcgtcgca caaagccagc gcgatcgcat ggcagtaggc
     3601 cgcatgctcg gcgagcaagt cgaattcgaa agtgcgctgg cgcagcacgc gagcgagcaa
     3661 catttgagcc tcatcagcca caagtgccac aggctgttgc tgaagcagca tgacgctaga
     3721 catgtgaaga agctccagca ggaagtcgct tgccgcggcc tctcccagaa cgtcatcgag
     3781 acgatcgccg acccgaaacg cctcgctgtc caccgcttgc atagccctga agaccagctg
     3841 ttccccaccc gccaggagcc atgccatgtg cccccattcc tcgtctgccg cacgacgcag
     3901 aagagcttgc gcctgtgatt tacgcacgaa gttctgcagg cgcttgcggg cacggttcca
     3961 gtcaatgacg aagacgatgc gggatgcgac ggcttcgaga ctgtctctta gtttttcgtc
     4021 atccgcggct ttgagtttgg cctttcccac ctggtatggc ttaccggcat tgaggccttc
     4081 ggaggttacc gggtcgtaca ccgtccactc aaaaccaatg ccctccagca tctggcgaaa
     4141 aaaatcaaag cgaccggcat gcaaatctga atacgtcagg ctgatggtgc gttgttccac
     4201 ctcaatgacc aggacatgga catcgttggt gccgatgtcg ttctggatca ggagttttga
     4261 gccattgcgg gttacagcgg tgtccaagcc tgggtgggag aacttcagtg gtgctgttcg
     4321 ctgcaggccc tgcatgaacg ccttgatgat cagtcggtcg gcttcgtcga tctgccagac
     4381 atgcgcacca tcaatgtctt cggtggcgat ctcgctggcc atggcgttga gttgcttgtg
     4441 caagtcca
//
LOCUS       DAIDFG010000262         4443 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_262_length_4443_cov_15.604603, whole genome shotgun sequence.
ACCESSION   DAIDFG010000262 DAIDFG010000000
VERSION     DAIDFG010000262.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_262_length_4443_cov_15.604603"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          775 a         1389 c         1461 g          818 t
ORIGIN      
        1 attgcccaag ctgcagcgcc ttctggtggc gccttttcag ctgcagcgca agctgatcga
       61 aaaaatcgat gccctggcgg aagccgctgc gctcggcaag gacacacgcc tggtggccaa
      121 gatgaacgcc ctgaccgacg aaggcctgat gctggccctg gtccgggcag ggcaacgcgg
      181 cgtgaaaatt gacctggtgg tgcgcggcgc ctgcatgttg cctgccggcg tcccgggggt
      241 gacggacaac atccgggtgc gttccatcat cggccgcttc ctggagcatt cacgcgtgtt
      301 ctattttcgc tgtgacgggg aggaaaccct gtacctgtcc agcgccgact ggatgaaccg
      361 caacatgctg cggcgcgtcg agctggcctg gtcggtgacc gatcccgcgc tgcgccagcg
      421 catcattgac gaatgcctgg tcgcctacct gcatgacagc caggacgcct gggacctgca
      481 gcccgatggc agctatttgc aggcgggcgc atcgcagcgg ggcgagcaac acggcgcgca
      541 ggctgcgctg atggcccgtt actcggccgg cgacgccgtg atggaagcct agcgatggac
      601 ctgatcctgt ggcgtcatgc agaggctgaa gagtcgcctg ccgacgcgca gggcgggaac
      661 gacctcgaac gttcattgac accgagaggc gagaagcagg cggcccgcat ggcggcctgg
      721 ctggaccgcc aattgccgga gcgggcgcgc attctcgtga gtccggcgcg ccgctgcgag
      781 cagacggcgc tggcgctggg gcgcaagtac aagatccgtg ccgaactcgg gcccgacgcg
      841 ccgccgtcgc aactgctcga actggtgcaa tggccggtca acaagctgcc tatcctggtg
      901 atcggccatc agccgacgct gggacaaacc atcgcccagt tgctgggcct ccaggaaagc
      961 gaatgcgccg tgaagaaggg ggcgctctgg tggctgcgca cgcgtgagcg cgaagagggg
     1021 ttgcagaccg tggtggtcac ggtgcaatca cccgaactgc tctgagccac gcctgccggc
     1081 tgggctgcgc tggttcagga agactttgtc gggcgaccgg tcttgatcgc cggcacattg
     1141 gccaacgccg tcagaaaggc tgcatcctgc atggctgcca gtgccttgat gccggccctg
     1201 agtaattcac tttttttgac cggctgggtc aacttggcag cgcgctgctt gagctcgtca
     1261 agcaccacat attcggcctt gggaatggtg aagctgtcgc gtaccagttt gggtttttta
     1321 accttctcgg gtttttccga cgcggtggca gccctgggct tgatcgtctg gtttttgacg
     1381 ggggcggtcg gggtcatggc ctttttggcg acaggggctc tggctttcgt gacgggtgtt
     1441 gccgctttcc tgacgggcgc cttgcgggag gcggtgccgg ctttcactgg gaggccggat
     1501 tttttcgcag ttgccatgtg aatgtctttc ggagtaaaca gtatatacag tttataccgt
     1561 tcttgaaaaa caggtcaagc cccgtccctc aggccttcag agtccttcga gcgccagact
     1621 ccagggcgtt ttctgccagg ccagcacttc ttcacgcagc aggtgcgctg actgggggta
     1681 ggcctcggcc caacccggtc ggcagaccag cgaaaactgg cgattaccgg cgctggcgat
     1741 ctgcacgccc ttgacatcgg ggtcgcgacg cgcgtggcac aggatcaccg ccagccgcag
     1801 gcaaagcagc tgcagcacga aaacctcgtc ctcgaaatcg gcctccagct tgcgcagctt
     1861 gccgcggtgg cccagcacca gcaggctcag gcggtgcagt tcctgcaggg tgaagcccgg
     1921 tgcatcggcg ttgtccagga tgtaggcgcc gtgcttgtgg taatcgctgt gcgagatgtg
     1981 gctgccgatt tcatgcagca gggcggccca gtcgagcttg cgctggacac gccccagttc
     2041 ggccggcgac aggccggtgc tggcttgcgc gaagagggcg cgggcgatcc tgctcacgcg
     2101 ctgggcctgc gcggtgtcgg tgtcgaactt gcgggtcagc cgctgcacgc tggtcgagcg
     2161 caggtcggtc gtttcttcct cgcggtcgag caggtcgtac agcacgccat gacgcagggc
     2221 gccctgggcg gtctgcatct gcttgatgcc catcagctcg aacaccgcgc gcagcacgct
     2281 gacgccgccg ccaatcacgg cacgccggtc gtccttcatg ccttcgagct tgaggcgctc
     2341 tgcgctctgg gccttgagca ggcggtccag cagccagtcc agcccttcgc gcgtgaccag
     2401 gccctggggc cagcccccgg ccatcagcac atcgctcaca gcaccgaccg tgcccgacga
     2461 gccgtaggcc acgtcccagc cgccgcggta gagattgatg gcttcgtcca gcactgcctt
     2521 ggcggccacc tcggccgtct ggaaggcccg cgcggtgaac tggccgtcgg ggaaatactt
     2581 catcgaccag cccacgctgc cgacgcggta ggactccatg gtcaccgcgt ccaggccctt
     2641 gcccaggatc atttcggtgg accggccccc gatgtccacg accaggcggc gttcgccgga
     2701 ctgcggcagc aggtgcgcca cgccctggta gatcaggcgg gcttcctcgc gtccggagat
     2761 cacgtcgatg ggaaaaccca gcaccttgtg ggcgcgctgc agaaaaacgt cgcggttgcg
     2821 ggcttcgcgc agggtctggg tggcgacggc gtgcacctcc ccctttttga agccggcgag
     2881 gcgctcgcca aaccgcgcca ggcagtccca gccgcgctgc atggcttccg gcgtgaggtt
     2941 gcgatcctca tccaggccgt tgccctggcg cacggtttcc ttgaggtatt cggtacggtg
     3001 gatctggccg tgatcgagac ggcctatttc gaggcggtag ctgttggacc caaggtccac
     3061 ggcagccagg cgggttccat tttgcatgtg tgagtgattg tttggggcgc agccagcata
     3121 gcattcacgc ggaacgacag tgccgggacg ggcatggcgg caggggcccg gaagggctac
     3181 ctgccgttgg gaaacgacac aatcaggcag agtgccgggc gccggtgacc gggctgtgaa
     3241 acgcggccaa tccgggctgc ggacaggggg ggggccggtt gtgggagcca cgaatgaccg
     3301 attggtaaaa tcgggtcctg ttttttcatg cgtttgtcac gtacctgtca cattgagctt
     3361 ctacagtaca gcctgttgac ccgtttatcc aaccgtaagg agccacatga aacacaagcg
     3421 cattttcctc aaagcaatgg ccgtcgccgc ttttgccact ctgggcatgg gttccgccat
     3481 ggccgccgac atcaccggcg ccggcgcgac cttccccttc ccgatttacg ccaagtgggc
     3541 cgaagcctac aaaaaggcga ccggcaccgg cctgaactac cagtccatcg gttcgtcggg
     3601 cggcatccgc cagattcgcg ccaagacggt tgctttcggc gcgtctgacg cacccgtgcc
     3661 cggcgcggac ctggacaagg atggcatggt ccagtttccc gccatcatcg gcggcaccgt
     3721 gcccgtgatc aacctcgaag gcttcaagcc tggcgaactg cgcgtcaccg gccccgtgct
     3781 ggccgagatg ttcctgggca aggttgtcaa gtggaacgac cccaaactgg tagccctgaa
     3841 ccccggcaag acactgcctg accagaacat caccgtggtg caccgcgctg acggatccgg
     3901 cacgaccttc aactggaccg actacctgac agtggtcagc aaggaatggg ccgacaccgt
     3961 gggcaagggt gctgccgtga agtggcctgc accgtcttcc gtcggcggca agggcaacga
     4021 aggcgtggcc gccaacgtga accgcgtcaa gggcgcgctg ggttacgtgg aatacgccta
     4081 cgtgaagaag aacaagatga atttcctgca gctgcagaat gccgatggca agtatgtgag
     4141 cccggacgat gctgcatttg cctccgctgc cgcgggtgcc gactggttca gcgtgcccgg
     4201 catgggcgtc tccatggtca atgccaaggg cgcagacagc tggcccgtga gcacggcttc
     4261 cttcatcctg atgtacaaga acccagccga caaagcgcag tccgccgagg tgttaaagtt
     4321 ctttgactgg gccttcaaaa acggcaaagg catggcttcc gagctcgact acgtgccgct
     4381 ccccgacgcg ctgactgccc agatccgcac caaggtgtgg tcgcagatcg cgaagtgagt
     4441 tga
//
LOCUS       DAIDFG010000263         4419 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_263_length_4419_cov_13.940422, whole genome shotgun sequence.
ACCESSION   DAIDFG010000263 DAIDFG010000000
VERSION     DAIDFG010000263.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_263_length_4419_cov_13.940422"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1125 a         1211 c         1153 g          930 t
ORIGIN      
        1 accagcacga gtttgcgcgg ctcaacctga cctatgtgct gaccagcaaa cgcaagctcg
       61 cccaactcgt caacgaaaac cgcgtgaccg gctgggacga cccgcgcatg cccaccatcg
      121 tcggcctgcg ccggcgcggc tacacacccg aagcgattca attgtttgcc gaacgcatag
      181 gcgtgaccaa aagcgacagc tggattgact acagcacgct ggaaggctgc ctgcgcgaca
      241 cgctggaccc gagcgccccg cgcgccatgg ccgtgctcga ccccgtcaag ctggtgctga
      301 ccaactggga cgaggtcatg ggcgccggta aattggacga ctgcagcgcc cccgtgcacc
      361 cgcaccaccc cgacatgggt acgcgcgagt tcaggatcgg caaggaagtg tggattgaac
      421 gcaccgacta cgaagaaacg ccacccaaag gtttcttccg cctgttcccc gcccacacca
      481 acgccagcgg ccaggccatc gcggccagca aggtgcgcct gaaatacggc cacgtcatcg
      541 aatgtgtggg tgcgaccaaa gacgcggcgg gcaacatcac cgaagtgcaa gccaaactga
      601 tccccgacac caaaagcggc acccccggct ccgacagcgt caaggtcaag ggcgtgatca
      661 cctgggtcgg cgtcaacgac gccgtgcagg ccgaagtacg gctgtacgac cgcctgttta
      721 cccaggccca ccccgaggcg gacggcaagg actttctgga aaacctgaac ccggacagcc
      781 tgagcgtggt gacggcttat gtggagcctt cactggcgaa tgttgaagcc ggccagcgtt
      841 ttcagtttga gcggcacggg tactttgtca ctgacttgaa ggatcacgct gcgggtaaag
      901 ccgtatttaa tcgggtggcg ggtatgaaag actcttgggg taaataaaat gcgcaatgca
      961 cgcaaacagc gacgcagtta aaaacatagg actaaaaatg gccgtgaaga cgcgacagta
     1021 cccatgcctc atttcaaaat ttcaaggaat tgtggtctac acatttgcca tgaaggtcaa
     1081 agatgcggtc ttcatgcact acgtcgcagc ccgaggggtt caggaggaag agggtgcggt
     1141 acaaaggtta ctggtaactc ggcgtgtcaa cgcaattcgc gatttcgtgc ttaaaaacaa
     1201 tggctttttc aacaccttta ttcttaattg gaccaatagc acggacaagc caaaaattca
     1261 aaaggctcaa cgccaaatcg aattgccgct tattgcccga tctgcccaag ttatagacgg
     1321 tcagcacagg cttgctggac tggagcgcgc aatgatgcag gaccccacgg ttggtgagag
     1381 agaaatcttg gtttcgttag cgcttggcct gacaaccgct gaagccgcct ccatattttt
     1441 aaacatcaac accgaacaaa agcctgttcc gaaaagtctg atttatgacc tatttggaga
     1501 agtagacaca gatccgaatc acttaattaa tcgagcaagt gatctggcaa cggagcttca
     1561 tgagaacccc gaatctccgt actaccaatt aattaaattt cccggcctat caaaaggcgc
     1621 cgcggcaatc gatctatcga ctattgtgtc cgcgctcaag ccacatttag gtccggacgg
     1681 agcatttacc aaagtcaacc tcaaaagtct tcagtaccaa aaatctacgt tactcaattt
     1741 ttttatcgcg ataaagtctt tttacgacga cgaaggtttg tgggaaaaca agggaaagaa
     1801 tcccttcctg cgagcttcgg ccttttccgg cgcaatagac ttcttaacag gaacgctgtt
     1861 gcctgcgtgt gcaagttcga aaaatttctc aacggaaaaa atgcgtgaat cgattcgact
     1921 tgacgtcaca ggtctcttgt tgatggaaga tgtcaaacag ctagatggaa aatcagcccg
     1981 cagagttgca aaagaccatt tggaaagtta cttgaaagat aagacgcctt cacaagagga
     2041 atatgagttc tgatcgcctg caattccaga catgggttga gaaggagctg ggctgtacaa
     2101 atcttactca gtggacgaat ttcaaaactc aactgaacgc attcacacta gggaccccac
     2161 agttagcaga agctcaagcc gcaatagctg ttgatgcaat tagtcttttc acgaaggcct
     2221 tggtgtcatt ctgcggcggc gttcatgaca tcgccaatgg tcatattaat tggggactag
     2281 ttaagcttta ctacagtctt ttttacagtt cgagagccaa cttatgcgcc aaacgtcaag
     2341 gtatggttcg taatcagagc tggtaccgga ttgatctaac tagtgccaat agaaccgcaa
     2401 tccgactcgc tggtaaccgc tacagaaatg atcacgacgc tacattacat ttatatgaag
     2461 atctataccg tggcggggac ccgttgctta gcaactcagt cgataacaag actccatacg
     2521 aatggatgat ggagacaaga aatttggtgc actacagact tgcgcttttt tcggaccccg
     2581 atttcccaag tcacataaag ccaacattta agctttccaa cctttctgaa atatctacac
     2641 agcttgatgt gtatctcacg gatgctacga acgcatttac attccagcct gaacacgctt
     2701 ggttagcaat tccattcaag cagatcatcc agacaagtcg tgaaattaga accagaagaa
     2761 tcgctcattc tttgcctgcc ttacagttct taaatattga gtctatatct cgttttctgt
     2821 cgacagaagg acaggctggg cttgaactgc agtcaattct ttcagctcaa gaataaagtg
     2881 actggctaat ttcaacgaca tgatgtcgtc cgctatcaaa ccgatgctgg ttgaaatatg
     2941 caaggctaaa gcgcgcgata cgggcgtggc tgacaatgcc gagtttcagg gacagcactt
     3001 cttcaagact gaacgagcac ctgcaaccgg gtaattcggg cccgaaccgc caatcagcga
     3061 cttttttcaa ccgggttggc cgtcaaccaa ttcgccggat aagcccgcat gaactcccgg
     3121 gccttctcgg gccgggcgct gagccaggca tcagacgcgc cctcgttcaa tatcgccacc
     3181 atgcgttttt cgttgccggg ctgctggcct cggctcatca gggcgtggct gttggcgttc
     3241 accgtgagca ggtgtagctg acgatcacgt cgccctccgc ccccgtccag ccttcccaca
     3301 aatcagcccc tcctaggctg gacctcaagt ttttggtgcg acttgccgtt attcaataag
     3361 aggctccgac aaggcctcag caggagcttc cagcgcatcg gctggttttc gcgcccagcc
     3421 gcgggggccg gtgaatgcga agtgtttgaa gtcctgcgca tcccgcctgg atggattgaa
     3481 ccatttaaac ggcatcggcc accgccgaac cgccacagct ctttctgtga aattagaaaa
     3541 aagggaagtg caccatgaaa ctccgaacca agatgatttc cgtgatggcg ctggtttgcg
     3601 cctgtttcac tttggccatt ggtgtggcgc tggtgggcat gcagaacgcg aaaagccagt
     3661 tcgagaattt tctccagcaa gaccaggcct tgcttcaagc cgggggcaat ctgtacgccc
     3721 agggcttgca gatggggcag gccttgcgca atatcgtgct cgacccggcg aataaaaagg
     3781 cccaggcaaa tctggacgcg gccagcgacg aattcaaaac ctcttacgag gcggcgatat
     3841 ccctttcaaa aggggatggc ggtacattga aaactctgca gaaggttggc gaactgcgtg
     3901 acaagcaggc gctgctccag aagcagattg ccgagctcgc ccagagtgat acgaatgctg
     3961 ccaggagcct gctgaacacc gcagaaacac ccgtctggcg ggacatgcgc gcgcgtctgc
     4021 aggagttcat caaggccaaa accgaggccg ccaaaagcac cgaagcagaa ctgcgccggt
     4081 tcacacagca gatgctgctc atcagtgtgg tcatggcggg cgtggccatt ctgctgggtg
     4141 tcggcatcac cttctggctg atccgcgatg tgatgaaaca actcggtggt gaacccgact
     4201 acgcggtggc gattgcaagc agcatcgctg ccggcgacct gaccatggat gtgcagaccc
     4261 gcgcaggtga cgaatccagc ctgttgtttg ccatgaaaca gatgtgcaac agcctggccc
     4321 gggtggtcgg cgaagtccgc agcggcaccg acaccatcgc cacagcatcc agccagatcg
     4381 ccgccggcaa ccacgacctg tcgtcccgca ccgaagagc
//
LOCUS       DAIDFG010000264         4416 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_264_length_4416_cov_18.008484, whole genome shotgun sequence.
ACCESSION   DAIDFG010000264 DAIDFG010000000
VERSION     DAIDFG010000264.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_264_length_4416_cov_18.008484"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          714 a         1506 c         1570 g          626 t
ORIGIN      
        1 catgcaagtc ttcatccact tctttgcaag gtcaccccat gaaacccttt ctccgcaaaa
       61 cactgttggg tcttttcggc ggcgcgctca tcgtcggcag tctgggcgcg tgttcacatc
      121 ggggtcccgg cggctggcag tccggtaccg aggcttcgcc ggaatcgcgc gcgcgcatgg
      181 tcgagaaaat cggctcccgg ctggacctgg acgcggcgca gaagcagaag ctggcggtgc
      241 tggccgacaa gctgcaggcc cagcgcgcgg cgctgcgtgg tgccagcgat ccgcgcgccg
      301 acatgcgcgc cctgttcgcc ggcagcaagc tggaccaggc cggtgccaag aagctgattg
      361 acgaaaaaac cgccgccctg caggccggca gcccggcggt gattgccgtt gccgccgact
      421 tcttcgacaa cctgcgtccc gagcaacagc agaaggtgcg cgagttcatg gaacgcggcc
      481 gccgctgggg ccgccatggt tgagccgccg gacgcggtgc atcaggcaac cttcttatga
      541 tggcgacatg ccccgcatcc tgctgatcga cgacgacgaa cacctggccg caccgctggc
      601 gacttacctg gcgcgtttcg gcctggtgct cgacagcgcc ctgcgacccg ggcagggcct
      661 ggccatgctg cgtgccacga cgtacgacgc ggccatcctc gacgtcatgc tgccggagat
      721 ggacggcttt gcgctgtgcc gcgagatccg caaggagagc gacattccgg tggtgatgct
      781 gaccgcgcgt ggcgacgtga tggaccgggt ggtcggcctg gagctcggtg ccgacgacta
      841 cctgcccaaa ccttttgagc cgcgcgagct ggtggcgcgc ctgcagacca tcctgcggcg
      901 ccagcgggtg gcgcccgcgc cggcgcgcca gccgcgccgg gtgttccagg atctggtgat
      961 cgacctggag tcgcgcgagg tcctgcgcca ggggcagccg gtggacctga cgggcaccga
     1021 gttcgagctg ctcgcgttgc tggccgccga gcccggcaag gtgttcagcc gcgacgacat
     1081 cctgaaccgg ctgcgcggcc acgaggcgga cctctacaca cgcgccgtcg acatcgtggt
     1141 gagtcggctg cgcaagaaac tggagccgct cgactgcatc aagacactgc gcaacgcggg
     1201 ttacacgctg gccgtgggca aggttgcggc gccatgacac atccacaggc cataccggcg
     1261 gcccgccgcc gctggcggca ttcgctgcgg ctgcggctgg tgacggtgtt cctgctgctg
     1321 gcgctggcca tggcggccgt gttcatgggc ggcatgcagc ggattttttc caccggctgg
     1381 cgtgatgccg cccggccgct ggtcgcggac tacatggacc ggctggtgca ggagatcggc
     1441 acgccgcccg acgtggcccg ggcccatgcg ctggccgaac ggctgccgat tgccatccgc
     1501 atcgaggggc cgcaggtgaa ctggcagtcg cacccggacc ggccggagca ccgcgagcga
     1561 gccggttttg gcggccgcgt gggggactgg ctcagccgcc ccacggccga cggccaccgc
     1621 gtgagttttg gcctgggcac cgtaccctgg cagcgtgggc cgcacagccc ggttggctgg
     1681 atcacgctgg cgctgctgct ggccctgatt gcggcggcct atgtttatgt gcggcgcctg
     1741 ctgcggccgc tggacgacat aggtgcgggg gccgagcgtt tcggccacgg cgacttcgac
     1801 gcgccgatcc cgctgcgccg gcgcgacgag ctgggcgacc tggcgcagcg catcaacacc
     1861 atggcgcagg acatcaaggg catgctggac gccaagcgcg gcctgctgct ggcgctcagc
     1921 cacgagttgc gttcaccgct gacacgcgcg cgcctcaatg ccgagctgct gcccaccagc
     1981 gccgacggca gcgccgcggg cgagcgcgaa cgcgccgcgc tgctgcgcga cctgaacgag
     2041 atgcgcgacc tgatcagcga cctgctcgaa agtgaacgcc tggccagccc gcatgcgacg
     2101 ctgcagcgcg agccggtgga cctggccgcg ctggtgcgcg aggtggtggc cgacatgccc
     2161 gacgctgccg ctgtgacgct ggacctggcc gcgggcctgc ccgcgttggc gctggaccgc
     2221 acacgcatgc gcctgctgct gcgcaacctg ctcgacaacg ccctgcgtta cagcgcggag
     2281 gccgaccagc cgccgcgcat cacgctcacc cgccaggacg gcggcgtgct gctggaagtg
     2341 cgcgacttcg gccccggcgt gagtgccgaa cagctggccc gcctgaccga acctttctac
     2401 cgcaccgaca gcgcacgcca gcgcgccacc ggcggcgtgg ggctgggcat gtatttgtgc
     2461 cagttggtgg cgcaggcgca tggcagccgg ctgcaggtgg acaacgccgc gaccggcctg
     2521 cgtgtccagt tgctattgaa agaatagctg ctgacgcccg tccacaaagg gctgagacgc
     2581 tattttgttc gtgttcccag cacggcggca ccaatgatca tggccgccgc caggccgatg
     2641 ctccagctca gcgcgcggcc gctgaccagc atcagcagcg cggtcgagcc cagcggtgtg
     2701 acgtaactca ggatgccgat ttgccgcgca tcgccgagct tgagcgcctt gtcccagagg
     2761 aaaaacgacg cgcccagcgg gcccagcccc atcaccgcga tcagggtcca gtcccgcccc
     2821 gacagcgccg cggacggctc cagtgcccag tggcacagca gcgacagcag gcccgagacc
     2881 aggccgaaca ggccgatggc cgcggtcgga aaggctggca cgcgttgggt cagcagcgaa
     2941 taactggccc agatgaaggc cgagcccagc gccggcaggt aaccccaagc ccagccgccg
     3001 ccggcgctac cggtgcgcgc gccgagaatg gcgaccgcgg cgccggcaaa acccagcagc
     3061 gcggccagca catgggcggc gcgcagcttc acgccgcgca ggaacagcgg cgccagcacc
     3121 accatgaaca gcggccacag gtagttgacg aggttggctt cgaccggcgg cgcgtggcgc
     3181 agtgcgatga atagcaggaa atgaaaaccg aacaggccat acacgcccag cagcagcgtg
     3241 ctggccggaa tgcgccattg ccgcctgtcc ttgagcacaa agggcagcgc aagcaggctg
     3301 ccgaccagca gggcgacgcc ggtcagcaga aagggcggaa cgtggcgcag cgacacgccg
     3361 agggaggcga ggctggccca cagggcgatg gcgcccaaag cgtacagatt ggctggcatg
     3421 gcgcaagcat agtcgcgggc cgcgcaccac atcgtcgcgc acgccggatc aaccgtctca
     3481 aaacaacggc gtggctgttt tcagtggggg cgcaaccggc gcagggcggc ggtcagcgcc
     3541 gagggcgact ggtagccgca gcgctgcgcg gtctcggcca cgctgaggcc accgtcacgc
     3601 agctgcacgg ccagcgccag ccgctggctg cgcagccact gcatcacgct gaggccggtt
     3661 tcgtcacggc aacgttcggc aaaccgggag ggactcagaa acacctgctg cgccagttgc
     3721 gccacgctca gtgcttcgtg caggtgttgc cgtgtccagt gggtgagccg ggcccagtcg
     3781 atgggccgct cgcgccgcct ggatgcgggg ccgggaacgc gccacgcgtc cagcaacagc
     3841 agcgggccgt agtgctgcgc caggctggcc ggctgcgcca gcgcctgtgc caggtagtgg
     3901 gcaagcgcca gggcttgcgc cggccgtgcg ggctggacgg ggcacgcggc ccagcttgtc
     3961 tgggcggtgt ccagcaccag gcagcggctg ccgctgcggg cttcgaagtc gtggcgttct
     4021 ccgggagcaa tcacgcagcc ctgacccgag ccaatacgct ggccgcggcc ctggacttca
     4081 agctccagca cgccgtccag gccgagcagc acctggaagt gcgcatgcgc atggctgccc
     4141 ggcgaggcgc cgtactggcg cagcgacagc gagccctgcg gcgtggccat gtcagacggc
     4201 gtcggacacg ccagcggcgt gggcttgctg gtcggcgtgg taactcgagc gcaccatggc
     4261 gccgaccgcg gcgtgggtga agcccatctt gtaggcctcg gcttcgaaca tcttgaaggt
     4321 gtcggggtgc acgtagcggc gcaccggcag gtggtgacca ctgggcgcga ggtactggcc
     4381 gatggtgagc atgtcgatgc cgtgtgcacg catgtc
//
LOCUS       DAIDFG010000265         4393 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_265_length_4393_cov_21.553020, whole genome shotgun sequence.
ACCESSION   DAIDFG010000265 DAIDFG010000000
VERSION     DAIDFG010000265.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_265_length_4393_cov_21.553020"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          800 a         1307 c         1436 g          850 t
ORIGIN      
        1 gacatcctga tcggcgggct gggcaacgac aaatactggg tggactcacc cgacgacctg
       61 gtggtggaga acgcgggcga aggcaccgac accgtctatg ccagcgttcc ctacgccctg
      121 gcagacaacg tcgaacgcct gaccctgaca ggcagcgcca acatcaacgc tgttggtaat
      181 acgctgaaca accgtctcga aggcaacgcg ggcaacaaca tcctgttcgg cgggttgggc
      241 aacgacacct acgtgtgggg tcgtggcagc ggccaggaca tcatcgtcaa cttcgacgcc
      301 ggcaagccct cgggcgacac cgtgcaactg ggggccgata tcgccgagac cgacctgggc
      361 ctcatccggc agggcaatga tctgatcctc agcgtcaatg gcaccaccga ccagctgacc
      421 gtggccaatt acttcgagaa tgccggcaaa ggtgccaacg cgttggagaa aattcgcttt
      481 gccgacggca ccagctggaa ccatgccgct gtgctcagcc gcaccagtgc agaagaaggc
      541 gcctcctctg cccagatgct gtcaccggag gtgctggccg gcaacccgac cgccctgttt
      601 gacgcccccg atccggcgca aaccaaaacc agcgatgccg ccacagcacc ccaaagcgtg
      661 gccgagtcga tcactgccgc caaagagcgt ttcgagcagg gcttgcagaa cctcacctac
      721 agcgtggacg agcagggcag cctgagccgt agagagtttg ccgagcggcg ggccttgccg
      781 ctgctgtgga acctgcagga tgccttgctc aacttgcagc tggccaagaa tccggacggg
      841 cgtttcacag ccgacatctc gatggacagc cgagctacgc gtgacctggg cctgggcata
      901 gcggtgttgg gcggggtggc tggcacggct gggcagctca ggcaggtggc taggcctcag
      961 gagatccagc agttcgacct cgctcagatg cagtaggtgt caggcgaaat tcaccagggc
     1021 ctgggaaccc tcatggagta tttgttgagc ccggtgcaga aggtgtcggc tgaggcggag
     1081 cgggagcggc gccaggaggc aagacgtgaa agcgctatga aatatgtagc ttttcacgtc
     1141 catgcaataa gggctgaggc cgaattttgt actcagccag aagtgaagcc aaacgaaata
     1201 ttgtccagaa cggtcaggca ctgtgcgtcg tgcaggagtc ggcaaacagt aaactgcagc
     1261 catggattcc gcccccctgc ctttgccagc tgacagccct cacgtcgtgg cggacatcgc
     1321 cgcgcgtgat gactgggtgg aggccaagct gacgcgcaac ttcatgcgca acgcgcggcc
     1381 ggcgctcaat tcaactgcgc tggtgatccc ggtcatcgtg gctgtcctct acaacaacgt
     1441 caaccattgg gcgttgctga gctggacgct ggcggcagtg cttattctgg tgatgcgcta
     1501 ctcggtggtc cggatctacc agcgcgacta tgccgactcc ggcatcgccc agcagcgggc
     1561 cttcatcgcg cgttatggct ggacttggcc tttgggtggc atggtctggg gcgcgtcgat
     1621 gttcctgtac tttctcaagg cgccggtctt tgaccagttc atctgcctga tcgtgctggt
     1681 gggcatggcg ggtttttccg tgggtgcgtt gtctgcgtgc ctgcgctgct ttatcgggta
     1741 catcaacggg ctggcctaca ccatcctggc cgcgttggcc tggagcatgg cggtagaacg
     1801 gcccttcccg ggcaccctca cgacctatgc gctggtggcg ctggtgctga tttactggtc
     1861 ggccgtccgc atggccgggc aacgcctgaa ccgcgttcag cggtccaacc tggatctgca
     1921 gtttgccaac gcgcagctga tcgcctcgct gaccgaaaaa acacgcgtgg cgctggaggc
     1981 ggtcgaagcc aaaagccggt ttattgcaag tgctgcccac gatttgcgcc agccggtgca
     2041 tgccctcggc ctgtatgccg actggctgcg cgccgagccg gaactggtgc tgcagatcac
     2101 cccgaaaatt gtgcagtcca cgcaggccgt caacgagctt ttcaattcgc tgtttgatct
     2161 ggccaaactc gatgccagcg tggtcgaggt gaactggcag cgggtggacc tgccgtcact
     2221 ggtcggggag ctggagttgc gctacgcccc gctggcgaag gagaaggggc tggacctgcg
     2281 cacccatgtg ctgccggggg cggtgcgctc agacccggtg ctgctcaagc gcctgctcgg
     2341 caacctgctg tccaatgcga tccgccatac cgaacggggc ggcgtgttga tggcggttcg
     2401 tccgggcgag gacggctggc gtgtggaggt gtgggacacc ggcgtgggca tcgccccgga
     2461 gcaccagcag gcgatttttc aggagtttta ccgcgtggcc aggcacaacg gaaccgagga
     2521 aggcttcggg ctggggctgg ccatcgtgac gcggctgtgc ggggccttga atcatcgcct
     2581 gagcatgcga tccaagcctg ggaagggcac ggtgttccgg ctggacatgg aggccttcga
     2641 cgacggagag gctacccgcg gacgcccaga cgacagccac cactgaagcg gcgccctgga
     2701 gcaggcccca gtgcaggtcc tagagcaggc cctgggtgcg ggcgaggtgg ctggcctgtg
     2761 agcggctttt gacattgagc ctgcgaaaca ggcgccacaa atgaaccttc acggtgtgtt
     2821 cgctgatctg caattcggcg gcaatgtcgc ggttgctcag gcctttgtcc agcatcacca
     2881 gcaactgctt ctggcgtttg gacagcttct cgggttccgt gctggcctcc ggatcggcac
     2941 tgtcgtccag aaaggttttc aggaccttgg cgatctcggt cgcgccgacg gatttttcaa
     3001 tgtagacatc ggcgccggct tccagcgaga ggtcctggta gtcctgcgcg ggggcggctg
     3061 acaacaccac gatgggcacc tcgggaaact gctggcgcac ctcccgcacg cctgatacgc
     3121 catgggtgtc cggcagtttg aggtccaggc agatgatgtc cgggacacca tgctgttcca
     3181 cggccgggcc gatggccgcc agcctgtcca gctcgatgac gacggctttg gtatgcagac
     3241 ggcgaataag catcaccacg gcttcccgca tgagtggatg gtcatcgatg acaaaaatac
     3301 gcatggggcc tggcggtaag gatgtgaaag actgtcagtg aatggaacaa aaagtaagca
     3361 tacctgctga ctgtcaagtt ggcgagtaag cgataaattt taagtccgga attaattcac
     3421 gatgcatggt ttgaaaggca tcctttttca ttacttgtcg gccttgcact tctgctgctg
     3481 gacgcgtttt gaggggcctc gtcccggcct tcaggacctc gcttcgacca gttgctgcaa
     3541 ggccgaatcc agtgctgccg cggtgatgcc ggggagaggc tgcagctttt cacgtaaacc
     3601 gtcgagtgcg ctggcgccct gggtttgcag caggccaatc gcctgcgcgg cctccttggg
     3661 agcatcgaag ccgtggcttt gcagcaacag ctggccctgg gtatcgacca gcttgaaatg
     3721 gaacttgccg tctgcctcgc ggtattgctt gaagaccggc agggaggccc tggctgcttt
     3781 ctctttgggg gcggcgctcg ccggggcacc gagcttgcgc aggcccaccg cgtggcgcag
     3841 ttcccgcaag aaaggggccg cgagctggcg tgccttgtcg gcgccggcct gcagaatggt
     3901 ttctatcctg gccggatcct tcatcagcgc ctggtactgg gctcgcatgg gcgccacgtc
     3961 ccggtcaatc cgctcgaaca ggagctgctt ggcatcgccc caggcaatgc cttcgccgta
     4021 ggcctggcgc agcgcggcgg tttcgggctc gctggcaaag gcctggtaga tctggaacag
     4081 cgccgagcct tcgatctcct tgggctcgcc cggtgcgcgc gagtcggtca cgatgccggc
     4141 gatgagtttt ttcagttgct cgcgcggcgt gaacagcggg atggtgttgt cgtaactctt
     4201 gctcatcttg cggccgtcca ggccaggcag caaggcgacg gactcctcga tcagcgcctg
     4261 cgggagcaca aagtgttcgc cgtacaggtg gttgaaacgc tgcgccatgt cgcgcgccat
     4321 ctcgatgtgc tggatctggt cgcgcccgac cggcacatgg tgggccttga acatcaggat
     4381 gtcagcgccc atg
//
LOCUS       DAIDFG010000266         4393 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_266_length_4393_cov_18.323190, whole genome shotgun sequence.
ACCESSION   DAIDFG010000266 DAIDFG010000000
VERSION     DAIDFG010000266.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_266_length_4393_cov_18.323190"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          636 a         1522 c         1496 g          739 t
ORIGIN      
        1 gtcggcggcg acacgacaca gggcccgctg aacatctgca tcaccgtgtt cggcgaggtg
       61 ccgcgcgggc aggcgctgct gcgttcgggc gcacgcgacg gcgatgaggt gtatgtcagc
      121 ggcacgctgg gcgaggcgcg gctggcgctg gaagcgctgc gcggcagcat cgccttgccg
      181 catgaacggc tggccgccgc gcgcacgcgg ctggaacagc ccacgccacg tgtcgcgctg
      241 ggcctggcgc tgcgcggcat cgcgacggcg gccatcgacg tgagcgacgg tctgctcggc
      301 gacctcggcc acatcctgga ggcctccggc accggcgccc gcatcgacac ctcggttgct
      361 ataaatttac tagctgatga cgcttatcca gcaagggctg agggccattt ttctcatgaa
      421 acccggctgc gctgcgcgct ggccggcggc gacgactacg agctggtctt caccgccccg
      481 gtgtcggcac gggcggtggt gcaggccgcc gcgctgcagg cgcagacagc ggtgacccgc
      541 atcggtgtca tcgaacaccc gcctggcctg cggctggtcg acgcctcggg ccatgcggtg
      601 ccgggccggt acgcgtcctt cgatcacttt gcctgagccg gtgcgcgctt gcgggcctgt
      661 tgcgtgtacc cgccatagcc ccaatcggtg gccggcagtt cccgcaccac cacgtagctg
      721 gcttcttcca gcgcgccgcc ctcgccgagc tggcgctgca gttcggcaaa cgccgcgctg
      781 acaaatgcgg ctttttcttc cgccgtgttg gttccggcgg tgatgctgat ctccagcagc
      841 gcggtgggcc gctgcacggc gtcgccaccc acgtaccaat gtccggcagg caggctctcg
      901 atgatgaccg ccgtcacctc gcgccgcttg ccgagcagct gtgcggtcag cgtggtcagc
      961 gcctgggcga gttgccggtc acgggccggg gtttgcagcg gtgccacttt cagcgtcagc
     1021 gtgggcatct caggcctccc gcggaagttg cagccttgcc gggcggcgca aggcctgccc
     1081 caactggcgc cagaagtcgt cgatcgcctg ccggcgcaag gcctcggcct ggcgcttggc
     1141 tgcatcgtgc aggcgctggt agctgtcatg gtggagatcg ctgtacatgg ccggtccttt
     1201 cgttgcggtg gctggtgcag gcaactgtag gcaaaccctc gcccgcggga aacggcgcgc
     1261 cgtgcaattg ggttttgcat tcgctgcaaa ggcggggatg ccggcggctg gttaccatga
     1321 cgccatgcgc gacctcagct tcgacgacat gcacctgttt gcccgcgtgg ccgagctcgg
     1381 ctccctttct gccgtggcgc gcgagcgcga cgtgccggtg agccaggtct cgcgcgcgct
     1441 ggcgcgcatc gaaaaagcct gtggcgcgcg gctgatccac cgctccaccc acggcctgac
     1501 acccaccgcc gaaggcgaga cctttttgca gtactgccgg cgcctgaccg gcacgctgga
     1561 cgagctcgaa ggcgagtttg ccagccagtc gcgccaggcc agcggctggg tacgcgtggc
     1621 ggccagcacg gtggtggccg agtacctcct gatccccagc ttcgaaagcc tgcacgcgcg
     1681 ccaccccagg ctgtgtgtgg aactgcaggt cgaggacagg ctggtggaca tggcgcacga
     1741 cggcatcgac atcgccatcc gcaccggcac gccgaccacc gacacggtgg tggcacgcca
     1801 gctcggcatg ctgggtcgca agctgtacgc cacgcccggc tacctgcagg cccacggcac
     1861 accgcgccat ccggacgatc tgcaccagca ccgcctgatc acgaacagcg cggtcgccat
     1921 gctcaaccgc tggcctttca tcgtcaaggg cgagccgagc gtagtggtgg cggagggcca
     1981 gtggcgttcg ggcagcaccg gcatcaccgc gcagctggcg ctgcacggcc tgggcatttc
     2041 gcgttttgcc accgtggtcg ccgagcccct ggtgcggcaa ggcctgctgg tgcccgtcct
     2101 gcccgaattt gtggacgagc agcccagccc gatttacgcc gtcacgctga cgagccggca
     2161 ccgcctgccc aagatcaggg cctgcctgga ccactgggcc gggtggtttg cccaggccga
     2221 gggcgttcag cccgcggtgc aggtataacc ccctgatgca agccgacacg tccaccgccc
     2281 cgccccccga agtccggcgc ctgctggacc gcgccacccg actcgaaacg ccctgcggca
     2341 gcggcagcat ggtctggcac cgctggcggc ccgatccggc cccgaccggt gcgccgcatc
     2401 ggcccgtggt gctgctgcac ggcggcagcg gcagctggac ccactggttg cgcaacatcg
     2461 acgcgctggt gggcagcgga cgcgaggtgc tggccgccga tctgccgggt tttggggatt
     2521 cggcgccgcc cgccaccggc aacgacgccg acgcgctggc cgcgccggtg caagcgggtc
     2581 tgcaggttct gctgggcgat gccgcctgcg acctggtggc gttttccttc ggtggcatgg
     2641 tcgccggctt tctggcggcg cagtggccgg cccgcgtggc gcggctggtg ctcatcggtt
     2701 cgcccggctt gggcgtggcc tcgcgaaaca ccgtcacgct caccgcctgg cgccacctgc
     2761 ccgacccggc cgaacgcgag gccgtgcacc gcaagaacct cgccgcgctg atgctctacc
     2821 ggcccgaggc catcaccgcg ctggcgctgc ggatacacag cgacaacacg ctgcgcgatc
     2881 gcatgaaggg ccgccgcctg gcttacaccg acgtgctggc gcgcacactc gccgaggtgc
     2941 gctgtccggt gtacgcgatt tacggccgcg aagacgcgct gtaccgcgaa cggcaggaag
     3001 cgctggcatt ggccctgcag tcggcgccga attttcgcgg catgcactgg atagacaacg
     3061 cgggccactg ggtacagttc gagcagccgc aggcctttga tgccgtgttg cgggcagtgc
     3121 tggaagaaag ccccgcccct tgaaacgcca tgaaaaacat agctatctgc gcacaccctc
     3181 attgggctgc gtgccatttt tacgcatgaa ataaaatcgc ctcgccccca gcgcaccctc
     3241 ttcccacaca ccatgccccc actgaacgaa acccctgcct ttccgggcgt gccaaacccg
     3301 gcgccaccgc gccggcccac ggcccgcttc atgctggcgc acccagccca cctgctggcg
     3361 ctgggcttcg gctcgggcct gagcccggtg gcgcccggca cggccggcac cttgtgggcc
     3421 tggctggcct ggctggcaat ggcgccctgg ctgagcacgc cgcagatggg gctgctgatt
     3481 gccgtggcca cgctgctggg ctggtgggcc agcacggtca cggccagaaa catgggcgtg
     3541 gccgatcccg gcagcatcgt ctgggacgaa gtcgtggcct tctggctcat cctctggctg
     3601 gtcatgccgg cgggcctgtg gggccagctc gcggcctttg tgctgttccg cttttttgac
     3661 gcggtgaagt tcggccccat ggcatgggcc gacaggacct tcaagggttt tggctggcgc
     3721 ggtggtttcg gcatcatgct cgacgacttc gcggctgcgt tctgcacgct gctggtgatc
     3781 gccgcctgga gcttctggtg aacttttcat cactatcaaa aggtgagctg cctgcgcccg
     3841 tcctggttgg gcttctggcc gatttgcttc tcaacaaaga ctggctgctg gccaccgccg
     3901 aaagctgcac tggcggtttg atcagcgccg cctgcaccga cctggcgggt tcgagcaact
     3961 ggttcgagcg cggctttgtc acctactcca acgaagccaa gaccgacatg ctgggcgtgg
     4021 atgcggcgtt gatcgccggg cacggcgcgg tcagcgaaga agtcgcgcgc gccatggtgc
     4081 agggcgccat cacgcattcg cgggcgcggg tcgccgtcgc cgtcaccggt gtggccggcc
     4141 ccaccggcgg cagccgggcc aaacccgtgg gcaccgtctg gttcggcttc atggtggacg
     4201 gccggctcag cagcgaagtc cagcactttg acggtgaccg cgccgcggtg cgccttgcca
     4261 ccgtgcagca tgcgctgcag cggctggtgc aactgctggg atagggcctg ctcacactgt
     4321 ttatgcaaga tgcgttgcga gtcaaaaagg tcatgcgcga ggcgcaaaac gcagccgggc
     4381 tggcgcccgg caa
//
LOCUS       DAIDFG010000267         4344 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_267_length_4344_cov_17.745628, whole genome shotgun sequence.
ACCESSION   DAIDFG010000267 DAIDFG010000000
VERSION     DAIDFG010000267.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_267_length_4344_cov_17.745628"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          853 a         1374 c         1415 g          702 t
ORIGIN      
        1 cgtctggagc tgcccgagct tcaacgaact cacgcgtgac ggccaggatg ccgagcgctg
       61 gaacctgctg cacccgctgg acacgcagcg tgttcccttc gtgggccagc agctcgaaaa
      121 acacacggga ccggtggttg cctcgaccga ctacatgaag gcctatgccg agcagatccg
      181 tcccttcatt ccccggggcc gtacctacaa ggtgctgggg accgacggtt ttggccgcag
      241 cgatttccgc tccaagctgc gcgagcactt cgagatcaac cgccactaca tcgtgattgc
      301 cgcgctgaag gcgctcagcg aagaaggcac cgtgccggtg gccaaggtcg cagaggcgat
      361 ccgcaagtac ggcatcaatg ccgacaaggt caacccgctt tacgcctgat catcacgcgc
      421 agaaaaatac ggacgaactg gagacaagaa catggcattg gtagacatac aggttcccga
      481 catcggggac tttgacgagg tgacggtcat cgagctgctg gtcaagccgg gtgacaccgt
      541 caaggcggag cagtcgctga tcacggtcga atcggacaag gcctcgatgg aaattccctc
      601 gagccaggcc ggcgtggtca aggaagtcag ggtcaagctg ggcgacaagg tcaagcaagg
      661 ctccgtcgtg ctggtgctcg aaggcgaggg cgctgctgcg accgcggcac ccgctgcgac
      721 tcccgttgca gaacaaaata aggccccagc ccccgctgca cctgcgccgg cagctattga
      781 ttcaggagcg tccacggccg cgccggctgc caccgccgcc gggccggtcg aggtccgtgt
      841 gccggacatc ggcgacttca aggacgtggc cgtcatcgag gtgctggtca aggtcggcga
      901 catggtcaaa gccgagcagt cgctggtgac cgtggagtcc gacaaggcct cgatggaaat
      961 tccgtcctcg gtgtcgggcg tgatccggga actcaaggtc aagctcggcg acaagatcaa
     1021 cattggcgac ctgctcgcca ttctggaagg cgtggcggct cctgccgctg ctgcttcggc
     1081 accagcaccg gcgccggcag cggcacccgc tgcagcagct gcggcgcccg ctgccagccg
     1141 ggccagcgcg ccggcgccag ccgccgtggc gccggtagcc ttgccggcgc acgaaccggc
     1201 tgcacccacg ggcaacctgc cgcatgcctc gccctcggtg cgcaagttcg cgcgtgaact
     1261 gggcgttccg ctgggggagg tcaagggcag tggccccaaa ggccgcatca cgctggacga
     1321 cgtgcagggt ttcaccaagg ccgtgatggc cggcgaagca cgcacccagg cgcaggcctc
     1381 caaagccccg gccccgtccg gcggcggcgc gggcctggat ctgctgccct ggcccaaggt
     1441 ggacttcacg aagttcggcc cggtcgagcg caaggacatg tcccgcatca agaagatcag
     1501 cggcgccaac ctgcaccgca actgggtcat gattccgcac gtctgcaacc acgacgatgc
     1561 ggacatcacc gaactggaag ccttccgcgt gctgatgaac aaggaaaacg aaaaggccgg
     1621 catcaaaatc actatgctgg ctttcctgat caaggcctcg gtcgcggcgc tgaagaaatt
     1681 ccccgagttc aatgcctcgc tggatggcga ccagctggtg ctcaagcagt attacaacgt
     1741 ggcgttcgcc gccgacacgc ccaacggcct ggttgtgcct gtcatcaagg atgccgacaa
     1801 gaagggcgtg gtgcagatct cgcaggaaat gggcgaactg gcgaaaaagg cgcgtgacgg
     1861 caaactcagc ccggccgaca tgcagggcgc gtgtttcacc atctcgtcgc tgggcggcat
     1921 aggcgggcgt tatttcacgc ccatcatcaa cgcgccggaa gtcgccatcc tcggcgtctg
     1981 caagtcgagc accgcgccgg tgtgggacgg ccaggccttc cagccgcgcc tgatgttgcc
     2041 gctgtccctg agctgggacc accgcgtcat cgacggcgcc tcggccgcgc gtttcaacgc
     2101 ctacctgggc cagatcctgg gtgacttccg ccgggtattg ctttgacccc cacgctcgtc
     2161 acttcgtgta cgtcgctgcc ccccgagggg gcgctgcgcc tgcggcccgg caaagccggt
     2221 tccgcggccc cggttggcaa agagcgtgcc cccgcgctgc ggccgctatg acgacgctgg
     2281 ttgccgtcag gaaagccggg caggcggtga tcgccaccga tgcgctgatc accttcggcg
     2341 atacgcgcct gggcagccgc ttcgaggcca acgagaagat cttcagggtc gagacaccgg
     2401 ctggcggcag ctacatcggc atggccggca cggcggcgca ttttccggtg ctgcgcaagg
     2461 ccatggcggc attgcccaag gaccagctcc ggctgggcag caaggacgag gtgttcgaca
     2521 ccttccacaa gctgcatccc gtgctgaaag acaccttttt cctgcagacc aaggaagacg
     2581 acaacgaccc ctacgagtcg agccagttca gcgcgctgat tgccaacgcc accggtattt
     2641 acgggctcta cagctaccgt gaggtgtttg agttcaagga gttctgggcc atcggctcgg
     2701 ggcgcagctt tgcactgggg gccatgcatg ccacctgggg caaggcgaaa acggcgcgcg
     2761 aggtggccct ggccggcgtg cacgcgggct gcgagttcga caagaactcc ggcggcccgg
     2821 tggaactgtt caccgtcaag ctgaaaaaat aagccgacgc atcaaacgga gacaagagac
     2881 atggcattga tagacattac cgttcccgac atcggggact tcgacgaggt caccgtgatc
     2941 gaactgctgg tcaagcccgg cgacagcatc gcggccgacc agagcctgct gaccgtggaa
     3001 agcgacaagg cctcgatgga gattccgtcc agccatgccg gcgtcgtcaa ggaactcaag
     3061 gtcaagctgg gcgacaaggt caagcaagga acggtcgtgc tgacgctgga cgccgaaggt
     3121 gtcgctgccc ccatctcaga gacaaaaaag gctgtaacca ccgctgtatc tgcgccggca
     3181 gctcctgaaa aggtagcggc gccagcaccc gtacctgcgc cagctgccgc cagctttggc
     3241 ggcaaagccg acctggactg cgacctgctg gtgctgggcg ccggacccgg cggttattcg
     3301 gccgctttcc gcgccgccga cctcggcctg aaagtcatca tcgtcgagcg ttacgccacc
     3361 ctgggcggcg tctgcctgaa cgtcggctgc atcccgtcca aggcgctgct gcatgtggcc
     3421 gcggtgatgg acgaggtcaa acatttcgac agcctgggcg tgagcttcgg cgagccggtg
     3481 ctcgacatcg ccaagctgcg cacccacaag gaaaaagtcg tgggcaagct gacggggggt
     3541 ctcagcgcga tggccaaaat gcgcaaggtg acggtggtgc gcggttatgg cgccttcgtc
     3601 ggcgccaacc atgtggaggt ggaagaaacc tccggtggcg ctcaggacaa aaccggcaag
     3661 aagcagacga ttgcgttcag gaactgcatc atcgcggccg gctcgcaggc ggtgcgcctg
     3721 cctttcatgc ctgaagaccc gcgtgtggtc gattcaaccg gcgccctggc gctgaaggaa
     3781 gttcccaaac gcatgttgat tctcggcggc ggcatcattg gcctggagat gggcacggtg
     3841 tattccacgt tgggtgcgcg cctggacgtg gtggaaatga tggatgggct gatgcagggc
     3901 gccgaccgcg acctggtcaa ggtctggcag aagatgaacg ccccgcgttt cgacaacatc
     3961 atgctcaaga ccaaaaccgt cggcgccaag gcgactgcca agggcatcga ggtgacgttt
     4021 gaaagtgctg agccgggagg ggcggtctcg gcgccgcaaa cctacgacct ggtgctgcag
     4081 gccgtgggcc gcacgccgaa cggcaagaag attgcggccg acaaggccgg tgtttccgtg
     4141 accgaccgcg gcttcatccc ggtcgatatc cagatgcgta ccaacgtgcc gcacatcttt
     4201 gcgatcggcg acatcgtcgg ccagcccatg ctggcgcaca aggcagtgca cgaaggccat
     4261 gtggccgctg aagtgattgc cggcgagttg cagggcaaca aggaactggc cagtgctgcc
     4321 ttcaatgccc gcgtgattcc cagc
//
LOCUS       DAIDFG010000268         4309 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_268_length_4309_cov_16.000470, whole genome shotgun sequence.
ACCESSION   DAIDFG010000268 DAIDFG010000000
VERSION     DAIDFG010000268.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_268_length_4309_cov_16.000470"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          719 a         1487 c         1422 g          681 t
ORIGIN      
        1 tcccgcccta ttgcaaggtg atctcgaccc ggcgcgcctc ggcgtcgctg cccgagcccg
       61 ccacctgctc gggtttttgc agcgtgatct ggctggctgt cacacccgct gctgtcagcg
      121 catcgcggac cgccagtgcg cgctggcggg ccagttcaac attggctgcg gcattgccct
      181 tggggtcgtg aaaacccgca atgaccacct tcttgccggt tttagctgcc ctgaccacat
      241 cgaccagcac cgcgccggca ccaggcgcca gatcggcctt gcccgacgca aaataaaatt
      301 tcaccacccc gtattccacc gtgatgctgg cggcatccga cacggcctgt gccgatggcg
      361 ctgttgccgg cgcagcggca ctggcaggcg cagccggcac cggcgcggcc agcacaggca
      421 acgcggcggc gcctgccacg gcgggcaagg ccggcgcacc ccggcgctgc agcgcggcgc
      481 ctatcaccaa cgcgatcacg agggcaatga tggcaaaaac aaggcccagg accgggccca
      541 gctgttcatc atccgatgag gaagacatgg ataaaactcc gtgaaactgc acaggctgcg
      601 gcgcctgtgc cggccgcaaa aaataaccgg ctgcttcgcc ggtgagagcc ggtcattgta
      661 aagtgtgact gtggaaactc ccccgcacca tgacctgtcc gccctgcgtg atccggcgcc
      721 catgctggag cgttttctgc accagtgggg cggccgagag gacctctgga ttttcggcta
      781 cgcgtcgctg atctggcggc ccgatttcga ctttgccgaa ctgcggcgcg cgcgggtgca
      841 cggctggcac cgggcgctga agatgtggag ccgcatcaac cgcggcacgc ccgagtgccc
      901 ggggctggtg tttgcgctgc tctcgggcgg cagctgccac ggcgtggtct accgtgtgcc
      961 gcgccacgaa gtgccggacg tggtggccag gctctggctg cgcgagatgg ccatgggcgt
     1021 gtatgacccg cgctctctgc gttgcaacac cgacaagggg ccggtccagg cgctggcctt
     1081 caccctctca cggcgcagcc ccagcttcac cggcgaactc agcgaggcgc gttaccggca
     1141 gattttctcc agcgcctgcg ggtgttacgg caccacacac gactacgcac gccagacgct
     1201 ggaaagcctg cgcagccacg gcatttcgga tgccaccctg gcccggctgc tcaacttggc
     1261 atcctgtcct acttcgcagg cctgacgcat ggcctagagt caagccgtgc ccgcttcgtc
     1321 cactgtaaaa aaggagttcc acatggtttc cagaatgctt ttgattgcgg ccgccgccct
     1381 gcttggcgcc tgtgccagca cccccagcgg cccacgcgcc accgcgcagt tgcagcccac
     1441 caccggcaac accaccagcg gcagcgtcag ttttgtccag aagggcaaaa aggttctggt
     1501 gagcggcgaa atccgtggcc tcaagccgaa tgcggaacac ggttttcatg tgcacgaaaa
     1561 aggcgactgc tccagcggcg acggcatgag cgcgggcggg cacttcaacc ccaccggcgc
     1621 gccgcatggc agccacggca tgggcatgca ccacaccggc gacctgccca gcctgaaagc
     1681 cgatgccggc ggtgtcgccc gcttcaactt tgaatcaggc tctctggctg tcggtagcgg
     1741 cgtgaccgac gtcgtgggcc gcggcctgat tgttcaccgc gaccccgatg actacaaaac
     1801 ccagccgacc ggcaacgccg gcccgcggct ggcttgtgcg gtaatacgtt agcccccacg
     1861 gtcgctcact gcgtgtagct ccctgccccc cgatggggcc gctgcgcctg cgggccggca
     1921 aagccggacc cgcggcccct gcttgattag ggaaatcaac gcgcggcgag cagtgcttcg
     1981 gcgcgcgcca ggtcgtccac ggtatcgaca tcggtcacga tgccgacatc gtcaagcgct
     2041 aggaccgcta ctgaattcat agcttcctgc gcccgcacta cgggcgctgc gccctgattt
     2101 cccgttaatc ccagcaaacc gtcccggcac gccgcggcaa agccgaccgg gtggccgcgc
     2161 gcgccctggt acagcggaat caccaccgcg tgttgcgcca gcgcccgggc cacggcccgc
     2221 aacgtgtcgc tgcgcaccag cggcaggtcg cccggcagca ccagccagcc cgtggcatcc
     2281 ggcgtggcac gcaccgccgc cgcaatcgag tcacccatgc ccggatgccc ggcgtcctca
     2341 aggtgccagg gcaggccgct ggcgatcact gcgtccagcg tgtgctgcag caccggcttg
     2401 ccggccagca gggcctgcag cttgtgggtg tggccgccgg acgcgatgaa acgttccccg
     2461 cggcccgagg ccagcacgat gacggtgggc agcgcaagag gcggggggga gacgggcgat
     2521 gaagacaagg acatggtgga ctacctatac ttccagtatg tcagagccta cccaacacat
     2581 cgccagcctg cgcaaaagct acgaacgcgc cgaactcaac gaagaggcct cgcatgccga
     2641 cccgctgcag caatttgacc agtggatcaa ggaagccatc gcggccgagg tgcccgagcc
     2701 caatgcgatg acggtggcca cggtggccgc caacggacgt ccttcgaccc gcgtcgtgct
     2761 gatcaaaggg tatgacgagc gcggcattgt ctggtacacc aattacgaga gccgcaaggg
     2821 ccaggagctg gccgccaacc ccttcgccgc gttgcagttt cactgggtcg agctcgagcg
     2881 cgtggtgcgc atcgaaggtc gggtcgaaaa agtgtccgcc gaggaaagcg acgagtactt
     2941 ccacagccgg ccgctggatt cgcgcatcgg cgcctgggcc agcccgcaaa gccaggtgat
     3001 ctccggccgc agcgtgctgg tggccaatgc ggcgaagtac ggcgcgaaat tcctgctgca
     3061 gccgccacgg ccgccgcact ggggtggcta ccggctcaag cccgacaact ggcagttctg
     3121 gcagggccgc aaaagccggt tgcatgaccg gctgcgctac acccaggtcg aggcgggctg
     3181 gctgcgcgag cgcctggcgc cctgaaacgg gtcgcctcag gcctcaagcg tccagacgct
     3241 ggatcgcctg atccgccacc gcctcgagct gcgcctgcgg caccagcgcc ggcgcaattt
     3301 ccgccagcgg caccagcaca aacgcgcgct gatgcatgcg cggatgcggc acggtgaggc
     3361 gggctgaatc gatgcggccg ctgccgtaca aaagaagatc gagatccagc gtgcgcggtg
     3421 cgttgcggta ggaccgctcg cggccggcgg cctgttccag ggcctgcagc tggcgcagca
     3481 gctcgggcgc gctcagggcg gtctgcactt ccaccacggc attgatgtag tcgccgccgg
     3541 cagccgggcc gggcgtgtcg cctgcgtcag ccagcggcgc ggtccggtac aggctggagc
     3601 ggcggctcac ccgggtcagc ggcaggcccg ccagggcatc catggccgac cgcaccgccg
     3661 cggcggcgtc accgaggttg gcgcccagcg cgacataagc cgtgacacca tcggaccgct
     3721 cagggcgcag ggccgtcccc atggccggct gacttagaag tcggtcgggg tgtcggaagc
     3781 cggacctgtc gatgcagccg agccgccttc gcggttggcc ggcttgcggc ggcgcctgcg
     3841 ctttttggcg ggcgcgggtt caccggattc gccggcttcg ggcgcaggtg ccgggccatg
     3901 gccggcatcg tcgctgcgcg gcgcgtcacc ttcggcgcgc ttgacgcggg tgcgcggcgg
     3961 ctgcggcttt ttcgactgtt cgagcctggc ctgctcgatc atctcgtggc gctcgtcgtc
     4021 gtaggcggtg ctgaatttct cccaccactc ggacagctcg ggttcgatct cgccggtctg
     4081 cgcgcgcagg cgcaggaagt caaagcccgc gcgaaagcgt ggctgctcca gcagcgaata
     4141 aggcgagctg ccggtgcgtt tttcaaaacg cggctgcatg gtccagattt cgcgcatgtc
     4201 ggtgcccagc ttgccgcgac ccgacacatc gccaatcttc gcgttgaagg catcgtcgat
     4261 cgcgtcctgc agcgcgggca tggtgttctc tccgcgcttg aggcgccgg
//
LOCUS       DAIDFG010000269         4303 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_269_length_4303_cov_20.269303, whole genome shotgun sequence.
ACCESSION   DAIDFG010000269 DAIDFG010000000
VERSION     DAIDFG010000269.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_269_length_4303_cov_20.269303"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          800 a         1479 c         1345 g          679 t
ORIGIN      
        1 gagcagaaga ttgcccaccc caaggccggc gccaacaccg cctgggtgcc cagcccgacc
       61 ggcgcgacgc tgcatgcgct gcactatcac caggtcaagg tcagcaaggt gcagaaagac
      121 ctcgagaaaa tcgacgccgg caaggagcgc aatgccctgc tgaacggctt gctgcaggtt
      181 cccgtcacgg ccacgccgga ctggagcgct gaggcgaaac agcaggagct cgacaacaat
      241 gcccagggca tcctcggtta tgtggtgcgc tggattgacc agggcgtggg ttgctccaaa
      301 gtgcccgaca tccacaacgt cggcctcatg gaagaccgcg ccaccctgcg catctccagc
      361 cagcacatgg ccaactggct gctgcacggc gtggtgacga aagcgcaggt caaggaaacc
      421 tttgaacgca tggccgccgt ggtggacaag caaaacgccg gggacccgct gtaccagccc
      481 atggccggtc acttcgacac gtcggccgcc taccaggcgg cctgtgacct ggtgttcaag
      541 ggactggagc agccgagcgg ctacaccgag ccgctgctgc acgcctggcg cttgaaggtg
      601 aaggccggca gcgcttgatt cactaccaag tcaatagctg tctgcgcccg tccaacaagg
      661 gccggggccg tatttgatgc acaaactacc ctccacggcg ccttcgggcg ccgtttttat
      721 tttccccatg ggagggctgc cgcctgttcg ggccaacacc cccccaccac agcgcgctgc
      781 gctgtggttg tcatctggcc gtaaccggct tcagcccgcc acggccggcc gccaatcgct
      841 cagcgcactg tccagtcgcg tgtccagccg ctgtagtcct tgtcggtcag cagcgtccac
      901 agggccagga cggcaatcgc caggccggcg accactgcat tgagcgtggc ctgctcatgc
      961 gtgttgaacc ccagtgccca gggcgagacg atcagccaga cgcccagggc cccctcggtc
     1021 cactcctccc aggcacgcgg cacaaaaatc gcccccagtg ccacggcgac cagggccgcg
     1081 ccgacgatga ccgcgttcag catgatcacc ctgtcatcct ggaaacccag cgcccagggc
     1141 gatagaagga cccagagacc caacaaggca ttgaccgggt cttgccagtg tttcacttgc
     1201 tccatggtta cacctcctga cagcggggaa aaacggggcc cgctgcagga aatttgaccc
     1261 ggcctggccc ggcattgttc cctgcggtcc ggcccagggc cttttgaaga ttgccccttg
     1321 aatccagtgg gtcatccccg gcgtattcct tccaggcgga caccacgccg acgcgctgtg
     1381 gcggattcca cagaccaaca accctcaaca ggagtctcac tatgaaaaaa ctactcattg
     1441 cagccggtgc tgccgttttg ctcagcgctt gtgccggtgg catgtccaac agccagatgt
     1501 ccggcggccc gtcggtcatg gtcggcggcg ccgccatgtt cccgaccaag gacatcgttg
     1561 acaacgccgt caactccagg gaccacacaa cgctggtggc cgccgtcaag gcggccggtc
     1621 tggtcgacac gctcaagagc cccggcccgt tcaccgtgtt tgcaccgacc aatgccgcgt
     1681 ttgacgccct gccggccggc accgtcgcca cgttgctcaa gcccgagaac aaggccctgt
     1741 tgacaaaaat tctgacggcc cacgtggtgc ccggcaagct tgacgctgca gcactgagcc
     1801 agcgcgtgat ggccggcaat ggccgcgcga ccctgaccac cgccagcggc gacagcctcg
     1861 tggtcacggc cagcggcccc aatgtgatgg tcaccgatga aaaaggcggc acggcgatgg
     1921 tcagcattgc caacgtctac cagtccaacg gcgtgatcca cgtggtcaac aaggtgctgg
     1981 tgcccaactg atcccgcgtt gcgctgaccc cgggggcggc gaacccgccc ctgcaaagcc
     2041 accacaaact gcaacggttt gttgtggctt tggccatccg gccccggtga cgcagaaaca
     2101 gggtgcgcca gccggcaggc tgcggcaccc gcacaggcct tcctgatttg ctatgtaaaa
     2161 catagctgtc agcgcttttc cttattgggc tcaagccgga ttttcttcac gaaccagcca
     2221 ccgaaggctg aagcaccaga agttgccccc gggcggcgcc agcggctacg gccacgcggg
     2281 tggaatgtaa gcagcgtctc tttgctgcgc ggttagttag aaatcgttac aagatacttc
     2341 ggcatttcca gcagcagccc gccagttcag cccacacttc ggggtcgggt cgaaccggaa
     2401 ttttttccca gaaaattgac ttgacacgtt ttcactctta cttctgttag ccacctcgcc
     2461 tctccaccat ggcatcgcaa gccgacgtct tactccgctg tctgcaggag gctgccaaag
     2521 ccgccaggcc ggcgcttgaa cgctctatcg accacgcggt ggcggcgctg cagctggcgg
     2581 agacacaaag cctgaaaatc accgagcgcg acgacctcgc actggcctgc cgcgagctgc
     2641 tgcgccaccg cgatggctgg tgtgcgcaat accccggggc cctgctggcc gccttcaacc
     2701 agcccgcaaa aacggctgcg cagccggttg cggcgcgcac cccggtgcct gcgctggcag
     2761 gcgccgacat gttctcgctg gtcgatgaca ccgaggtcga ggaaggcatc gagtcctcgc
     2821 ggctgctgca gcaggtcgcg cccatggtcg agcaggtgct ggccgaactc gacggcctga
     2881 tgagttcggc gcaggggctg gtcaacgtcc gcgcggaggc caacccggtg cggcccgagg
     2941 tgtatgcgca aacgctgcgc accctgctgc tggccgcgcc ggtcgcgccc accatcagcg
     3001 cgctgtggat caaacacctc ggcgagcccc tgggacgcga actcaaacgc atctacgaac
     3061 ggctgatcaa tgagctggaa ctggccaagg tgcgggccgc cagctaccgg gtgctgcaga
     3121 cgcccgccac caccggcaaa aagccgggca gcgccgcagc tggcgccccg gcgggtgaga
     3181 accccggcac cgggccgggc gcgcagggcg gccccgtcgg catcggttac gcctcggacg
     3241 aacccttcac ccgggtgccg tcgcagtacg cggacctgtc caaccacgac gtcaaggacg
     3301 cgctgttcca ggacttcctg tttcgcggcg gcagcaacgc cgatcacggg ttggcgcccg
     3361 cctactacgc caccattgaa gaagaactga tcgcgctcaa gggcacgccc gactcggcac
     3421 aggcggcgct gcaggccggg ccggccaccc ccgatactgg ccatgccggc gatgacgtcc
     3481 gaagctacca cggcacccgc agcgacccgc accgcccggc gctgccggcg caggacgccg
     3541 atgaggggcc ggacaacccg tcgcgcatcg tggacgtgct gagccagctc agctcgcagg
     3601 tctggggcgt ctatggccgt gcccgcgagc gggcgctggt gcgcacgcag ctcaggaaag
     3661 acgccacccg cgtcggccag gtgctgggta tggaggtggt gaaaaagctg gtcaaccagg
     3721 tcgcgcagga cccacgcctg ctggtgccgg tgcgcgaggc gattgtggcg ctggagccat
     3781 cactgctgcg cctggccatg gtggactcgc gctttttcag tgaggaaaac caccccgggc
     3841 gccgcctgat ggagcgggtg gcgcagcgca gcttcaagta caacgacgag cgcagcccgg
     3901 cgttcgacgt ttttttccat tcgctgaccg aggccttcaa cgggctcaac gcgctggaga
     3961 tcgaggacgc ccggcccttt ggcgccgcgc tggccgaact gcaggcgctg tgggacgaac
     4021 aggaccagca cgagcgcgat ctgcgcggca acatgcgcca gaccatgcag tttgccgaag
     4081 agcggcaggc gcaggccgac cagattgccc tggacatgag tctgcgcgcc gacctggacc
     4141 atgtgcccgg cgtggtgctg gactttctgt tcgggccctg ggcgctggtg atggcgcatg
     4201 cccggctgac cgacaccgcg cgccagatcg acccccaggg ttatggctcg ctggtgaccg
     4261 acctgctctg gagcgtcaag cgccaggcca cgctcaagcg acc
//
LOCUS       DAIDFG010000270         4292 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_270_length_4292_cov_16.914326, whole genome shotgun sequence.
ACCESSION   DAIDFG010000270 DAIDFG010000000
VERSION     DAIDFG010000270.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_270_length_4292_cov_16.914326"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          843 a         1389 c         1311 g          749 t
ORIGIN      
        1 gacagcttgg aggcacgcgc cagttcccgg ctgccctgcg catcgaccag cagcagggcg
       61 ttgtcgtcac ggccgaaagt ggccgggccg atgttgccca ccagcagcgg cacgcccttg
      121 cgcaggcgct tggcctgcgc gttgtcctcg aggtcgtggc tctcggcggc aaaaccgacg
      181 cagaacaact cacccgatac gccacgcggc gattgcgcca aggtcgccag gatgtcgggg
      241 ttctcggtaa aagccagctg gggtgcctgg cccgagccgt ccttcttgat cttgcgctcc
      301 gcgctgctcg ccggtcgcca gtcggccacg gctgcggttg ctataaaaac agtagctgct
      361 tgcgcccgtt ccgtaacggc ttcgagcatg ttttgtgctg atctgacgtc gatgcgggtg
      421 acgccgcgcg gcgtgctcag gctgaccggc ccggccacca gcgtgacctg cgcgccggcc
      481 tcgtgcgcgg cgcgggccac ggcaaaaccc atcttgccgc tcgacagatt ggtgatgccg
      541 cgcaccggat cgatggcctc gtaggtcggg cccgccgtca ccagcacatg ctgaccggcc
      601 agcaccttgg gcgtgaaaaa ggcgaccatg tcttcgagca gctcgggcgg ctccagcatg
      661 cgcccgtcgc cggtttcgcc gcaggcctgg aacccgctac ccaccccgag gatggtcgcg
      721 ccatcgctgg agagctgcgc catgttgcgc tgggtggcgg gatgggccca catttcacga
      781 ttcatcgccg gcgcgatcag cagcggcacg gtggcgatgg gccgggccag gcacatgagg
      841 ctaagcaggt cgtcggcacg gccatgcagc agtttggcca tgaagtcggc gctgcagggc
      901 gcaatcagga tggcgtcggc ctcacggctc aggttgatgt gcgccatgtt gttggcctca
      961 cggctgtccc actggctggt gtagaccggc cggccgctga gcgcctgcag cgtcaccgcc
     1021 gtgatgaact gctcggcggc ctcggtcatg accacctgca cagtggcgcc ctgcttgatc
     1081 agcgcgcggc acaactccgc cgccttgtag caggcaatgc cgcccgacag gcccagcacg
     1141 atgtgccggc cggccagatc gccgcctgct tcgatggctt cttcgtttgc ttgcttcgtc
     1201 atgctccgca atgtacagga tatgccccca cgcttgccac ttcgtgtgct gcgctgcccc
     1261 ccgagggggc tatttttcct aggggcggcc cgtcggaaaa atgtttgccc tggcctggtg
     1321 tgagcgtaga cgcatgcagc cacccgtata atccccgttt accacctaag cgaacttgcg
     1381 gttcgtacct gacatgacca aatttgtctt cgtcaccggc ggtgtggtgt cttccctggg
     1441 caagggaatc gcctcagcct ccctagcagc gatccttgaa tcgcgaggcc tcaaagtcac
     1501 tttaatcaag ctggacccct acctcaacgt cgatcccggc acgatgtcgc cgttccagca
     1561 cggtgaggtg tttgtcaccg acgatggcgc ggaaaccgac ctggacctgg gccactacga
     1621 gcgttttgtc gaaacgcgca tgcgcaaggc caacaatttc acgaccggcc agatctacaa
     1681 aagcgtgctc gaaaaggaac gccgcggcga ctacctcggc aagaccgtgc aggtcattcc
     1741 gcacgtcacc aacgaaatcc aggaattcat caagcgcggc gccggttacg acacgccgca
     1801 ggcggtggat gtcgcgattg tcgaaatcgg cggcacggtg ggcgacatcg agtcgctacc
     1861 cttcctcgag gcggtgcgcc agatgagcct gcgcctgggc gccaacaaca cggcctttgt
     1921 gcacctcagc tatgtgccct ggatcgctgc cgcgggcgag ctcaagacca agccgaccca
     1981 gcacaccgcc aaacagctgc gcgaaatcgg catccaggcc gatgtgctgc tgtgccgcgc
     2041 cgaccgcccc atccccagcg aagagcgtga aaaaatttcg ctgttttcca acgtccccga
     2101 gtggggcgtg atttcgatgt gggacgtgga cacgatttac aaggtgccgc gcatgctgca
     2161 cgagcagggt ctggacgggc tgatctgcga caagctgcgc ctgaacacgc ccccggccaa
     2221 cctcaagcgc tgggacgacc tggtttacga aaccgaacat ccgaaaaatg aagtgcacat
     2281 tgccatggtc ggcaagtacg tggacctgtc ggacagctac aagtcgctca acgaagcgat
     2341 tcgccacgcc ggcatgaaaa accatgccaa ggtcgtcatc gagtatgttg attcggaaac
     2401 actgacgccg gacaacgtct cgcggctggc caagttcgat ggcatcctgg tgcccggcgg
     2461 tttcggcaag cgcggcattg aaggcaagat ctgcgcggcg cgttttgcgc gcgaaaacaa
     2521 ggtgccctac ctcggcatct gcctgggcat gcaggtcgcc accatcgagt ttgcacggca
     2581 tgtggcgggc ctgaaggacg ccaacagcac cgagttcgag cccgacagcc cgaacccggt
     2641 gatcgcgctg atcaccgagt ggaaagacga agacggcacc atcaagacgc gcaacgaaca
     2701 gtccgacctc ggcggcacca tgcgcctggg cgcgcaaagc tccgacgtgg ccaaggacac
     2761 cctggcgcac aagatctacg gcgacgtggt caccgagcgg catcgccacc gctacgaggc
     2821 caacgttcac tacctggacc ggctgcgtga agccggcctg gtgatctcgg cactgaccca
     2881 gcgcgaacac ctgaccgaaa ttgtcgaact gccgcagaac gtacacccgt ggttcatcgg
     2941 cgtgcagttt caccctgaat tcaagtccac gccctgggcc ggccacccgc tgttcaacgc
     3001 ctacgtgaaa gccgcgctgg accaccaggc ggccggaaaa acattgaaag tcgtggcatg
     3061 aagttatgtg gcttcgacgt gggcctcgat cagcccttct tcctgatcgc cggcccctgc
     3121 gtcgtcgagt ccgagcaact gcagatggac acggcgggaa cattgaagga aatcacggcc
     3181 tcgctgggca ttcccttcat cttcaagtcc agctacgaca aggccaaccg ctccagcggc
     3241 accacctttc gcggccccgg catggaaaaa ggcctggaga tcctggccaa ggtcaaacgc
     3301 gagctgaacc tgcccatcct gaccgatgtg cattcggagt ccgagatagc cgccgtggcg
     3361 gccgtggtgg acgtgttgca gacgccggcc ttcctgtgcc gccagaccga cttcatccgc
     3421 gcggtggcgc aatcgggcaa accggtcaac atcaagaagg gccagtttct cgcgcccggc
     3481 gacatgaaaa acgtgatcga caaggcacgt gccgcagcgc gtgaaaaagg cctgaacgaa
     3541 gacaacttca tggcctgcga acgcggcgcc agttttggct acaacaacct ggtcagcgac
     3601 atgcgcagcc tggccatcat gcgcgagacc ggtgcgccgg tggtgtttga cgccacccat
     3661 tcggtgcagt tgcccggcgg acaaggcacc agcagcggcg gccagcgtga gatggtgccg
     3721 gtgctggccc gtgccgccgt cgcggtcggc attgcgggca tcttcatgga aacccacccg
     3781 gacccggcca aggccatgag tgacggcccg aacgccgtgc ccctcaaaca catgaaggcc
     3841 ttgctggaga cgctgctcga gctcgaccgc gtcaccaaga aaaacggttt tctcgaaaac
     3901 aacttccaag cctgatcatg accagtgcct acatcattgc caacgtgacg gtcaccaatc
     3961 cggtgcagta cgaggaatac aagaagtggt ccagcgccgc catgcaggcg catggtgccg
     4021 aagtctgcgt acgcggcggc aaggtcgaag tgctcgaagg cgactgggcg cccgagcgcc
     4081 tcgtcatcct gaagtttccg acgtttgaag cggcgaaggc ctttgacgcc tcccccgaat
     4141 acggcaaagc ccgggccgcg cggcaaggcg cggccatcat gcggatgatc atcgtcgaag
     4201 gtgtctgacc cttcgcctgc cagtttttaa tcttgatttg aaagacctcc atgagtgcaa
     4261 ttgttgatat cgttggccgc gaaatcctgg ac
//
LOCUS       DAIDFG010000271         4273 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_271_length_4273_cov_19.298246, whole genome shotgun sequence.
ACCESSION   DAIDFG010000271 DAIDFG010000000
VERSION     DAIDFG010000271.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_271_length_4273_cov_19.298246"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          800 a         1284 c         1372 g          817 t
ORIGIN      
        1 gtatgtcatg aagatcaacc ccgagaagat ccgtgacgtg atcggcaagg gcggcgccgt
       61 gattcgcgcg ctgaccgaag agaccggcac gcagatcaac atcgaggaag acggcaccat
      121 cacgatcgcg tcgaacgaca gcgccaaggc cgatgaagcc aaacgccgca tcgccgagat
      181 caccgccgaa gtcgaaatcg gcaaggtcta cgaaggcgcg atcaccaaga tcctggactt
      241 cggtgccctc gtcaacctgc tgcccggcaa ggacggcctg ctgcacatca gccagatcgc
      301 ccacgaacgt gtcgagcgcg tgaccgacta cctgagcgaa ggccagatcg tcaaggtcaa
      361 ggttctggaa accgacgaaa aaggccgcgt caagctgtcc atgaaggcac tgctcgaccg
      421 tccggcccaa ggccaggacc agggctgaac tgctatctga ttgatagctg ctcgcgcccg
      481 tctgacgggc gcttcggcca acaatgagca tgtacctgat gaaagcagtc gaaatcactt
      541 ccttcggacc gcccgacgtc ctgcgtctgg gcgagcggcc tgcgccggtt gccggcgaag
      601 gcgagttgct gatccgcgtc agtgccagcg gggtgaaccg ccccgatgtc ctgcagcgca
      661 ccggcaacta cccggtgccg cccggagcgt cggatattcc ggggctggaa gtggctggcg
      721 tggtggttgg cggcgacgcc gaggccatgg cgcaggccgg cctgaaggtg ggcgaccgtg
      781 tctgcgcgct ggtggcgggc ggcggttatg ccgagctgtg tgtggcgccg gtgggccagt
      841 gtctgccggt gccggcgggc ctcagtgata tcgaggcagc ctcgctgccc gaaacattct
      901 ttaccgtctg gagcaatgtc tttgaccggg tgcgcctgca aaagggcgaa accctgttgg
      961 tccagggcgg ctccagcgga atcggagtca cggccatcca gatggccaag gcgctgggcg
     1021 ccacggtgct ggtcacggct ggcagtgacg acaaatgcgc cgcctgcctg gccctcggtg
     1081 ccgaccatgc gatcaactac aagaccagcg actttgccga agaggtcaag aagctcacgg
     1141 caggtcaggg cgtcaatgtc attctcgaca tggttgccgg cagctacgtg gcgcgtgagg
     1201 tcgagtgcct cgccgaagac ggccgtctgg tgatcattgc cgtgcagggc ggcctcaagg
     1261 ccgagttcaa tgccggcctg gtgctgcgaa agcgcctgac cgtcaccggt tcgaccctgc
     1321 ggccacggtc catcgagttc aaggctgcga ttgccgcggc cctgcgggac aaggtctggc
     1381 cgctgattgt cagcggtgcg atcaagcccg tgatccacag cacgtttgct gcggcggagg
     1441 ccgcgcaggc ccatgcgctg atggagtcga accagcacat cggcaaaatc gtcttgacct
     1501 ggcaggtacc cagcccatga aaaaactcat cgcaggaaac tggaaaatga acggcagcct
     1561 ggctgccaac gaagccttgc tgcaggctct ggcggccggt tttccggggc agccggcgtg
     1621 tgcggtggcg gtgtgtgttc ctgcgccgta tctggcgcag ttccaggcgc tggccaaggc
     1681 cttgccggcg ctggctgcca ttgatctggg ggcgcaggac gtttcggcca aggcctccgg
     1741 ggcctacacc ggtgaagtca gcgcgtccat gctgaaggac ttcggctgcc gttacgccat
     1801 cgtcggccac tcggagcgcc gccagtacca ctttgagacc gatgcgctgg tggccgaaaa
     1861 ggcacagcag gcgctggcca tgggtatcac gccgattgtc tgtgtcggcg agaccttggc
     1921 agaacgcgaa gcagggcaga cggaaagcgt cgtcaaacgc cagctggcag ccgtgattca
     1981 tgccaatggc cactgcatca gtgaaatcgt cgtggcctac gaaccggtct gggcgatcgg
     2041 caccggcaag acggcgacac ccgagcaggc gcaagcggtg cacgcggtgc tcagggccca
     2101 gctcaaggcg gcgacggacc agtccgcgcg cgtgaaaatt ctgtatggcg gaagcatgaa
     2161 tgcggccaac gccgcaacgc tgctgagcca gcctgacatc gacggcggcc tgatcggcgg
     2221 cgcgtcgctc aaggcccccg attttttgac gattattgct gcagctgccg tctgacgggc
     2281 gaagcacgct attcaattag gagtaaacat gaacgttttg ttaaccattg ttctggcggt
     2341 ccagatgatc accgcgctgg gcatgatcgg cctgatcctc gtgcagcatg gcaagggtgc
     2401 cgacatgggg gcggcttttg gcagcggcgg ctccggcagc ctgtttggcg ccagcggcag
     2461 tgccaacttc ctgtcgcgca cgacagcggt tttggccggc gtgttttttg tctgcacact
     2521 ggccctggct tatttcggta acgcgcgtcc acagaccggc accagcgtgc tggaaggcgc
     2581 agccatcacc gcgccagcgc cggttgctcc cggtgccggc cagatccccg gcacggcaag
     2641 ccccgcacct gcagcgggtg cggttgccgt accgcctgca ccggcagctt ctggcgcagg
     2701 tcagatcccg acgaaatagc gccataaagg tgccgttctg ctcagggaac ggcacaaatt
     2761 cagggtaaac tctgagactg cccggaaagc caaaaccgca aggttcctca tgcaatccgg
     2821 acagctaaaa accgcggacg tggtgaaatt ggtagacacg ctatcttgag ggggtagtgg
     2881 cgaaagctgt gcgagttcga gtctcgccgt ccgcaccacc tacaggcact ggccagtact
     2941 gcgcaggcag ggcccaaggc cttgaacagc aaactggcac ccagcacaag cggcttgcag
     3001 aagttgaatc gacgatgaac ctcgaccaat acctccccgt cctgttgttt atcctggtgg
     3061 gcgtggctgt cggcgtggtg ccccaggtgc tgggtcgtct gcttggtccg aacaagccgg
     3121 acagcgaaaa aaattcccct tatgagtgcg gcttcgaggc gtttgaagat gcccgcatga
     3181 agttcgatgt gcgttattac ctcgtcgcca tcctgttcat tcttttcgac ctcgaaatcg
     3241 ccttcctgtt tccgtgggcc gtggcgctca aggagatcgg tccggtgggt ttctggtcca
     3301 tgatgatttt cctcggcatt ctggtcgttg gttttgccta cgagtggaaa aaaggcgccc
     3361 tggactggga atagctcacc aagactcagc ggctgcacgc gcagccgcca caaagggacg
     3421 ggtatgtcac tagaaggtgt tttcaacgaa ggcttcatga ccaccagtta cgactcggtg
     3481 gtcaactggg ccaagaccgg gtcgctctgg cccatgacct tcggcctggc ctgctgcgcc
     3541 gtcgagatga tgcattcggc cgctgcgcgt tacgacatcg gccgtttcgg tgccgaggtg
     3601 tttcgtgcca gtccgcgcca gagtgacctc atgattgtgg ccggcacctt gtgcaacaag
     3661 atgggtccgg ccttgcgcaa ggtgtacgac cagatggccg agccgcgctg ggtgatttcc
     3721 atgggttcct gcgccaatgg cggtgggtat taccactaca gctactcggt cgttcgcggg
     3781 tgtgatcgca tcgtgccggt ggatgtgtat gtgccgggtt gcccgcccac ggccgaagcc
     3841 ctgatctacg gcattatcca gttgcagcag aaaatccgcc gcaccaacac cattgcgagg
     3901 gcttgatggg tcctttgcat cctgctactg ctgaacagct ctccggcatc attgccgctg
     3961 ccttgggcga caaagccaag cgcaccacgg tggcgctcgg tgaggtgacc gttgttgtcg
     4021 atgtgtccga ttacaaggcg gctgccctga tcctgcgcga cacccgcggc tgccaattcg
     4081 aacagctgat ggacctttgc ggtgtcgatt attccgagta cgccgagggc cgctacgatg
     4141 gtcgccgctt ttgcgtggtg tcccatttgc tttctgtcag tctgaaccag cgtgttcgcc
     4201 tgaaggtctt ctgcgccgac gatgatttcc ctgtggtggc atccgtcagc gatgtctgga
     4261 actcggccaa ctg
//
LOCUS       DAIDFG010000272         4245 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_272_length_4245_cov_21.408592, whole genome shotgun sequence.
ACCESSION   DAIDFG010000272 DAIDFG010000000
VERSION     DAIDFG010000272.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_272_length_4245_cov_21.408592"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          829 a         1313 c         1344 g          759 t
ORIGIN      
        1 cgagcgcagc cgccacatca tcaaggcgcg ctggctgacc gaaggcgaat cggcgaccct
       61 gcacgaactg gcggctgaat atggcatttc cgccgagcgc attcgccaga tcgaagcgcg
      121 cgcgctgcaa aaaatgcgcg ccctgattcc ggcgtaagct gcgccgcgcc ccaatgaaaa
      181 cgccccgcaa tgcggggcgt tttcattggg ctgcagccga ttcagaagaa agaggcaatc
      241 aggaccgaca ggacgctgat ccaggcccag atgatcaatg cggtcaccac caccatgggg
      301 aaaccctgaa aagtgaaaag ctgtttcatc gtgcgcgctc cataaaagtt gattgatttt
      361 agtttacttg tgcccgtttg ggtacctgtt cccggcgggg ttttcgtctt ggcggctcgg
      421 gcggcgggtc ggcaacagcg ggtcgtcgtc gtccatcagg atgcgctcgg ccgggccttc
      481 caggtcgtcg aactggccgc tcttgatcga ccagaacacg ccgacgacga tcagcagcac
      541 aaagatgccg gacagcaaga acaaaaaccc catgatgccg ctcacggcgc ggccagctcc
      601 tgtatgagct gatagcgcat ctcgcggtcg cgtagcaggc gcagctcggc cagccagcgc
      661 ccaccggcgg gcacgtgcag caccccgcca taggtgccgg catcgacggc ggcgagcggc
      721 aatacgagcg cagcctccac cgccccaggg cgctgcaggc gcagctcggc ctcgatgcga
      781 tcgacgggca ggccggtgcg atccctgacg cgaacctgga tcgcgcgcgc cggctcgccg
      841 ctgtcgaggc catcgacttc gacctgccag ccgagctcgg actcgcgctg cgcctgagac
      901 aattcggacg agaccgcttt ggctgccgcc tgcccgtgtg tcaccacccc ggaaaagccg
      961 ctgtagaccg caccgccgtc gctgcccagc caccagcgcg ccagcggttc gggcagaccc
     1021 ttggtggcga ggtaaagcag gctgccgttg accacaacga ggcccaggaa aaacgccgtc
     1081 aggagcttgg gcacccaatg catgcggccg gcactgcccc ggcggaactg gctcaccgca
     1141 aacagcacca gccccgccgc aagaaacacc gagatgtgta tggcaaccac gtccaacccc
     1201 ggccagcgcc cgacaatgag cgcgaaatac acgagcagcg ggatcagtcc cgccagcagt
     1261 gcgcgcagga tcgggggcag gccgcgaatg acgcctccga ttgcataaag cacaaacacg
     1321 gccgccaggc cgccaagcag ggaggtcatc gtcgtcatgg tcgttttccg ggggcgtcga
     1381 aacggacgta ttcggccgtg gcttcagcgg gtttgccctg cggcgtgatg accagctcga
     1441 agcgctgggt tggcgccgcc accgccagag gcagccgcac gctcgcctgc accagtgcgc
     1501 tgtggccggg cttgatggtg atttcgctga aattgccgag atcgagcgcc tcggccggga
     1561 tgccgcgcgc gctgatatca taggtctgcg cctgcgctgc cttgttggtg atgcggatct
     1621 ggtagcggtt gcggatgtcg ccgttcgaca gcatgacgta gagcggctga cggatctggt
     1681 tgacggtgtg attgaagttg ccgcgcgtgg ccacgctgta gaccaggtag gcactcatca
     1741 ggaccagtgc catcccgtaa ccgatggttt tcagccgctt ccacttcagc gtcggcgctg
     1801 ccggcgtcgg cgaggccaga ttcttttccg agtcgtagcg gatcaggccg cgcggaaaat
     1861 tgaacgaatc catgatggtg ttgcaggcgt cgatgcacag cccgcacgag atgcacttgt
     1921 attgcaggcc gtcgcggata tcgatgccga ccgggcagac ctgcacgcac aggccgcagt
     1981 cgacgcagtc gccgaggcct ttttcgcggc gctcggccaa ggtgcgctgg ccggcgcgcg
     2041 cggcggcccg gccgtgtgcg gcttcgccac gtcggacatc atagtgcacg gccagtgttt
     2101 cgggctcgta catcacgctc tggaagcggc cgtacgggca gacataggtg cagatctgct
     2161 cgcgcatcag gcccgcagcg acgtaggtcg acagcgtgag gataccgacc gtgatgtagg
     2221 cggcgggcgc ggcctggccg gtgaagaagt ccagcaatag ctgcggcgcg taggcgaaat
     2281 agaaaacgaa ggtcaggccc gtccacaacg acgccagcat ccacagggca tgggtgccgc
     2341 cgaccttgag gaccttttcg cgatcccagg gctgacggta gaggcgcacc cgggccggac
     2401 gctcgccctg gaccttgtat tcgatccaga tgaagaagtc ggtccacagg gtctggaagc
     2461 agaagtagcc gcagaaagcc cgcccgacca gcccggtgac gaagaacagc agaatggcgg
     2521 cgatgaacag caaaagcgcc agccagatga catcctgcgg gtagaccgtc agcccgaaga
     2581 tcaggaagcg gcggccgggg aggtcgaaca aaaccgcctg cgccggggcg tccaggcgcg
     2641 accatggcag ccacggcagc agaaagtaaa tcgcataggc caacaccagc accgaggtct
     2701 tgaaacgccg aaacttcccc ttcaccgagc gggtgaagat gggaatgcgt ttctgataaa
     2761 gcccgagctg gtcttctaga cctgtttgca tggtgtgacc ggttgagtgg gtagtaagca
     2821 aaagcccccg ttcacggggg cctggatcat cgtgtcagcc agtgaccctg ctactgcccg
     2881 ccacccagtt catgaacata gaccgccaga accttgattt gttcaggcga caggcgtccg
     2941 gaccacgcag gcatcacgcc cttgttgaga ccgttgttga tgacgctgcg aatcgctgca
     3001 accttgccct cggccgtgcc ggctgccggc acgtcggccc atagccagat gttgtcggtc
     3061 aggttgggcg cgccgatgtc ctggcgtccc gttgcacccg cggtgtgaca gtaaaagcag
     3121 gctgctgttt cgctatggaa cagcatgtcg ccggcccccg ccttggcggc gtcgtgctcg
     3181 atgcccgaca ggctggccac gtagttcgcc agctggtcga tctgctcgcc attcagcact
     3241 tcgccgaagg ccggcatgta gccacggcgg ccgcccatca gggtttcatg aaccttctcg
     3301 taggaaccgc catacagcca gtcgtcatcg gtcaggttgg ggaagaagcc aaccacgccc
     3361 tggccgccgg cctggtggca ggccgcgcag ttttctgcaa agagcgacct gcccgcagaa
     3421 agaatgaagc cgctcatttc cgggtctttg gcaatctgct cgtagctcat cgcctggatc
     3481 ttgtcgaagt agggcttttg cgcgaccgcg gcctggttca tgtcagccac gagcagcgca
     3541 cgggtgttcc agctgtgggt cttcgtttcg cccgcgctgt tgacgtaggt gatgttcgag
     3601 acgccgccga tccagccctt gccaagcggc catgaggggt agatggtcca gtagatgagg
     3661 gagaagatca ccgtggcgta aaacgtgtag acccaccaca ccggcagcgg attgttgtat
     3721 tcctgcaggt cgccgtccca ggcgtggcct gtggtttgaa ccgtttgaga ttgtagtttt
     3781 tgctcgctca ttgcttgtcc ttcgtgccgt cttgatcgcc tggctggtca tcgaaaaacg
     3841 ggatgtcgcg gtaagactcc agtttcacgc tgcgcttctt gctaccgtag acgtagatca
     3901 cgatgccgat aaaggtgacg aaaaaaatga tcaacgccag gggtttggtg ttttcaggtc
     3961 tggaaaacca catcaaccat tccatttcct gccccctgcc gtgacgctta gcggaactcg
     4021 acgaacgcgt cgtcggtgta cttgctgaaa tcgaccatgg tgcccagcat ctgcagatag
     4081 gccaccagcg catccatttc ggaaatgccg gtgcggtcgc catcgtagtt gccgaagttg
     4141 gcctgctcga ccaggttctt ctgcgcatcg ggaatgtgca gttggctggc cacggtctcg
     4201 ccgaacttgg tcacgttggc gtcgtactcg gcctgcgaca gcgaa
//
LOCUS       DAIDFG010000273         4245 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_273_length_4245_cov_17.933652, whole genome shotgun sequence.
ACCESSION   DAIDFG010000273 DAIDFG010000000
VERSION     DAIDFG010000273.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_273_length_4245_cov_17.933652"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          830 a         1355 c         1336 g          724 t
ORIGIN      
        1 caagatgggg cgcgacaagg aaagccaggc catcctgccg ctgcgcggca aggtactcaa
       61 cacctgggag gtcgaacgcg accggctgtt tgccaacacc gagatccacg acatcgcggt
      121 cgccattggt gttgatccgc atggccccaa cgattccccg gacctcagcg gcctgcgtta
      181 cggcaagatc tgcatcctca gtgatgccga cgtggacgga tcccacattc aggtgctgct
      241 gctgaccttg tttttccggc atttccccca acttattgca acagggcaca tttttgtggc
      301 acggccaccg ctttttcggg tggacgctcc ggcgcgcggg aagaagcctg catcgaaggc
      361 gtacgcgctt gatgaaggcg agttgaccgc cattctggat aaattgcgca aggaaggcgt
      421 gcgtgaaggg gcttgggcga tcagccgctt caagggcttg ggcgaaatga atgcggaaca
      481 actgtgggaa accacactca atcctgatac gcgccgattg ctgcccgtgc aactcggtaa
      541 tttcgacttt gtgcagaccg agagtctggt gaccaagttg atgggcaagg gcgaagccgc
      601 ggcgcggcgc gaactgatgg aactgcacgg cgatgccgtg gagatcgata tttaaaggac
      661 ttccatgcgc aagctgcgga tctgcacggc atgagccatt ttttacgtcg aaagtgcctc
      721 cagcccttgt ggctattgcg caaacagcta tcatttttga tagctgctgc cgccctgttg
      781 ctggccggca cacctgcccg cgccgatgtc tggggttatg tggacggcca gggcgtggca
      841 catttctcgg ccgagcggct cgatgagcgt taccagctgt tttttcgcgg cggcgaaagt
      901 tttgacacca gccgcggtgg aaagacctcc tcgggcgcct acggcaatgg catgccgggt
      961 gcaccgccca agctgctggc ctttttcgat gtgtcaccca actacaaggc cgtcaaacac
     1021 ttgctgcgcg aggcgtcggt ggcccatgac atcgactacg aactgctgca ggcgctgatt
     1081 gccaccgagt cgggcttcaa cacccatgcg gtgtcgccca agggcgcggt gggcctgatg
     1141 cagctgatgc cgcccacggc ccaacggtac ggcgtgcgag ccgacaagaa ctccccgatc
     1201 gaaaaaaagc tgaccgaccc caaaaccaac attcgggccg gctcgcgcta cctgcgctac
     1261 ctgatcgacc tgtttcccgg ccagctggag ctggcggtgg cggcctacaa cgccggcgaa
     1321 ggcgcggtcc agcgttatgg caacaagatt cccaactatc ccgagaccaa aaattacgtc
     1381 aagaccgtga tgcagctcta cagccatctc aagccaccca gcatgatcgg ggaggcgcgg
     1441 cgctcgggcc gtgtccggat ggaaatgatg ggcggcagca aactgccgtc cagcctgccc
     1501 gctggcggcg ccaccggacg cggcaaccag ctgccgaccc tcgtcgtgca aagcccggtt
     1561 gcaccggaat tccgggtcga acgcgactag tgtcctgacg tggaaccgct gtttcgcgtg
     1621 aggccataat cgcctgcgac ggccagccgg cccatcggtc cggccacctc tttgaccccc
     1681 atcctttttt ttcagtttga acctcgatgg acgatctttt tacgcctgcc ccgccttccc
     1741 ctcccgacgt gcctgaccag gaatcgctgg ccgcctatgc ccagcgcgcc tacctcgaat
     1801 acgcgctcag cgtggtcaag ggccgggccc tgcccgacgt ctgcgacggc cagaagccgg
     1861 tgcagcggcg catcatgtac gccatggcgc gcatggggct gggcttttcc ggcgccggcg
     1921 gcgccaagcc ggtcaaaagc gcgcgtgtgg tcggtgacgt gctcggcaaa taccatccgc
     1981 acggcgacca ggcggcgtat gacgcgatgg tgcgcatggc gcaggacttc agccagcgct
     2041 acccgctgat cgacggccaa ggcaacttcg gcagccgtga cggcgacggc gccgcggcca
     2101 tgcgttacac cgaggcgcgg ctggcgaaaa tcaccaccct gctgctcgac gaactcgacg
     2161 aaggcacagt cgacttcgtg ccgaattacg acggctcttt cgaggagccc aagcaactgc
     2221 cggcgcgcct gccgttcacg ctgctcaacg gcgccagcgg catcgccgtc ggcctggcca
     2281 ccgagattcc cagccacaac ctgcgcgaag tggccgatgc ctgcatcgca ctgatcaaga
     2341 cccccaaact cagcgacgac gagctgtaca cgctgattgc cgggcccgac tacccgggcg
     2401 gcggccagat catctcgaca gccactgata tcgcggctgc ctacagcacg ggccgcggct
     2461 cgctcaaggt gcgcgcccgc tggaaaattg aagacctggc gcgcggccag tggcagctgg
     2521 tggtgcagga gctgccgccc ggcgtcagta cccaaagggt gctcgaagaa atcgaagaga
     2581 tcaccaaccc gaaaatcaag gccggcaaga aagcgctgag cctggaccag gtccagctca
     2641 agcagaccgt gctggccgtg cttgacgtgg tgcgcgacga gtcgagcaag gacgcggccg
     2701 tgcgcctggt gttcgagccc aagaccagcc gcatcgagca gtccgaactg atcaccaccc
     2761 tgctggcgca caccagcctg gaaagctcgg ccagcatcaa cctgaccatg atcgggctgg
     2821 acggccggcc gacgcagaag acgctgcggc aaatgctgca ggagtggata tcctttcgcc
     2881 agaccacgat agagcggcgt tcgcagcacc gcctgaacaa ggtgctggac cgcatccaca
     2941 tcctcgaggg ccggcagctg gtgctgctca acatcgacga agtcatcgcc atcatccgtc
     3001 agtccgatga gcccaaggcg gcgctgattg cgcgttttaa tctgagtgag cggcaggccg
     3061 aagacattct tgaaatccgc ctgcgccagt tggccaggct ggaagccatc aagatcgagc
     3121 aggagctcaa gggcttgcgc gaagagcagg gcaaactcga ggaaatcctc ggcagcccgg
     3181 ccgccttgcg gcgcctgatg atcaaggaaa tcgaggcgga cgccaaacag tttgccgacc
     3241 cgcgtcgtac cctgatccag gccgagaaaa aagccgtgct ggaagtgaag gtgctcgacg
     3301 agccggtcac cgtggtcgtc agcgccaagg gctgggtgcg cgcgcgcagc ggccacggcc
     3361 acgatgcggc cagttttgcc ttcaagtccg gtgacggcct gtacggtacc tttgaatgcc
     3421 gcaccatcga taccctgctg gtattcggca cggcgaccaa aggcacgggc cgggtgtatt
     3481 cgattccggt ggccatgctg cccggcgccc gcggcgatgg gcagccggtg accacattga
     3541 tcgaacttga aaccgggaca cacccggcgc attatttcgc cggaccggcc agcgccacct
     3601 tgctgctcag cagcagcggc ggctacggtt tcctggccac ggtcgagaac atgacgtcgc
     3661 gcctgaaggc cggcaaggcc ttcatcagct gcggtgaagg cgaggcgatc tgcaagccct
     3721 cgcatgtctc gggcaacagc ggcacggcgc ctttgaaccc ggcgacccat gtggcctgcg
     3781 cctccaccgg cgggcgcatc ctgaccttcg agatcagcga actcaaaacc caggccgccg
     3841 gcggccgcgg cctgatgctg atcgacctcg atgccaagga caccctggcc ggcgcggcgg
     3901 cgtacacccg cagcgtgaaa atcgagggca taggccgcgg cggcaaggag cgcgaggaga
     3961 cgctggagat ccgcagcctg aacaatgcca gggccgcgcg cgggcgcaag ggcaaggcgg
     4021 ccgatctggg cttcaagccg acggcgatca gccgggtcga atgacagggt cgtagttgct
     4081 atctgtttga tagctgctgg cgcccgtgga tattgggctg aggtgctgtt tcttatgaag
     4141 ccgttcgggc tgggcggggg taagcctctt tgatctcagc tgctatggct tgccagcaag
     4201 ccgggactcg ccccggcggg cgacttactt ttctttgctt cgcca
//
LOCUS       DAIDFG010000274         4213 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_274_length_4213_cov_13.987494, whole genome shotgun sequence.
ACCESSION   DAIDFG010000274 DAIDFG010000000
VERSION     DAIDFG010000274.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_274_length_4213_cov_13.987494"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          812 a         1263 c         1250 g          888 t
ORIGIN      
        1 atgtcgtggc gcaacccgga tgccagtgat cgcgatctgg gcatgcagga ctacctgcaa
       61 atgggcgtga tggagtccat ggccgccgtc aaatcgctta ctggtgcccc ccgcattcac
      121 gcgatgggtt actgcctggg cggaaccttc ctgtccatcg tggccgctgc gctgggtctg
      181 ccggaaaacg catcgcgctg ggggtacctg cccgaactgg ccacagtcgc cctgctggcg
      241 tcgctaaccg atttcagtga acccggcgaa cttggcgtct ttatagacga tgaccagatc
      301 cagaccttgc gcgagaccat gtcacgcaca ggctatatgc caggccggca gatgggtggc
      361 gccttccagt ttctgaattc ccgcgagttg atctggtctc gccagacaca ccgttacctg
      421 ctgggcaagg acgacgttgg caacgacatg atgagctgga acaccgacgt gacgcgcctg
      481 accgagcgca tgcattcgga gtatctgtct tcactttatc tagacaacgc gctggcctcc
      541 gggcactacc gtttgggagg cacaggcgta gccctgatgg acatcaaggc gcccatgctg
      601 gtagtgggca ccgtgcggga tcacgtctcg ccctggcgct ctgtctacaa gattcacctt
      661 ctcaccgata cccacactac gttcatcctg gccgccggcg ggcataacgc gggaattgtc
      721 tccgagccgg gccacccccg gcgcagctac caactgggca gcgtggaggc cggccacggt
      781 tggatagaag cggacgaatg ggttgcgact gcgcctgcgt ttgagggctc atggtgggag
      841 gccatggacg acttcctgaa agcgcgttca gggaaacctg tatctccccc agccatcaat
      901 ccaggcaaag ttctgggtga tgcacccggc aactacgtgc aggttcgtta tgcagattga
      961 caagccgagc ccctaggagc ggcaattctc ccggttcact ccttgtccag cgctcttcaa
     1021 cgcagtgaat tgctaccttc cgcgcgcagc gcacatttcg ttcacgcttc ccatcaggat
     1081 taatcccatg aatcgcgctg tacctcagga atacttcgaa aacgccatct acgaagagat
     1141 ccatattggt caaagcgcgc ggacggtgcg tacactcacg cgagaagaca tcaatgcctt
     1201 cgccgcggtt tcgggtgaca ccaatccggc ccatctggat ccggtctatg cggacgccac
     1261 cctcttccat ggtgttattg cacacggcat gtggggcggc gccttgatct cagccctgct
     1321 gggcactgtg tttccaggac ccggaacgat ttaccttgaa caggcgctgc atttcagcaa
     1381 accagtgcgc gtgggcgaca cactgaccgt gaccgtcacc gtgaccgcca aggaggacga
     1441 caagaaacgt ctcgatctgg attgccaggt tctgaatcag aaaggagaac gcgtgctcca
     1501 tggcatggcg cgagtcatgg cgccaacgca aaaaatacgt cgcccgcgct acgacgcccc
     1561 ccatattcaa ctgtttgatc cagaggctcg cctccgggct ctactcggaa tgggtgatgg
     1621 cctggccgct attcgatgcg gcatcgttca cccctgtgac gtggaatcat tacgcggcgc
     1681 cattaaatca gcccagctcg gcctgatcga ccccgtgttg ataggccccg aagccaaact
     1741 gcgggaaatc gcagaagagg ccaacatcga cttgggcggc gttgacattg aatccgttcc
     1801 gcacagtcac gcggcagcct gcaaagcgtc tgaaatggca gcagcgcgac aggtggaagc
     1861 cctcatgaaa ggcagtttgc acaccgatga gctgattggc gcagtacttg ccacgaccgc
     1921 cctgaggacg gctcgacgga tgtcgcatgt attccggttc gatgtgccga cttaccacaa
     1981 gcccctcctg ctgaccgatg cagcattgaa cattcatccg acgctagaac acaaggcgga
     2041 cattgtgcaa aacgcagttc agtttgccag gatcatgggg accgcactgc ccaaggtggc
     2101 gattctgtca gcggtcgaaa cggtcaaccc caacatcgtg tccactctag acgccgcagc
     2161 cctgtgcaag atggccgacc gggggcagat ttcaggtggt ttgctggacg gccctcttgc
     2221 cttcgacaat gccatctctc ctcttgcggc tcaaaccaaa cacatcgact ccaaagtggc
     2281 cggtgacgcc gacattctgg tggtacccga tctggagtcc ggcaacatgc tggccaagca
     2341 gctggaatac cttgcgggtg ccacggcatc gggcatcgtg cttggtgccc gcgttccgat
     2401 cgcgttgacc agccgggccg atggggcatc tgccagggtg gcctcggcct tgctggccaa
     2461 gctgattgcc caccacaacc ggatgattca gccgtgagat tgtcagagcg atttctcacg
     2521 ccgtcctcat gactgttctg actgtcaatg cggggtcctc aagcctcaag ttcgccttgt
     2581 acccggtgaa tggaacaatc gcggactctg cgacgctggc gggaaccgtc gaggggcttg
     2641 agtctgacgc accgctggcc ctgtcctggc gtgcggaaaa tcagagcggt caggagcaca
     2701 tcgacgtcat cgcagatgac ccgttcgagg atgcgctaaa ggccctccaa agcttgctgc
     2761 aacaggccct gccagggcgg gacttgcagg cggtggcaca ccgggtcgtg catggcgggg
     2821 aagcctaccg cgatgcagtc aaggttgacg tggacatttt gagcagactg agtgacctat
     2881 ccccgctggc tcccctgcat cagccccaca acatcgaagg gattcgtgcc tttggtcgcg
     2941 cttttcccga agtgccgcac attgcctgct ttgatacggc attccacgcg gggattcccg
     3001 acgtggagca tctttttgcc ttgcctcggg cgctgggaaa gcaaggtatc cgtcgttatg
     3061 gatttcatgg cttgtcctat caatacgtga tgcaaaagct caagcgactt tcggacagag
     3121 cccagggacg cgtcgtgatg gcgcatctgg gcaacggtgc cagtctctgc ggtgcgctgg
     3181 agggtcaaag catggcgacc accatggggt tttccgcgtt ggacggtctg atgatgggaa
     3241 cgcgtagcgg ctccctcgat gcgggggtgt tgctgcacct gatggcgcag ggctgggatc
     3301 atgcgcgcct gcaagacctg ctgtaccggc aaagtggctt gctcggcgtc tcaggcatct
     3361 cggccgacat gcgtcgtctg cgcgccagcg gggaagcgga tgccaatttg gccatcagaa
     3421 tgttcaccca ccgggtgatc cgcgaagccg gggccatggc cgcctgcctt ggcgggcttg
     3481 acgtgctggc cttcaccggc ggaatcggtg agcatgaccc tgtgctgcgg gctgaagttt
     3541 gtgaaagact gggctttttg ggcgtcgtcc tcgaccatga gaagaaccgg cagcataccg
     3601 cagcgcaggc ggtcagcctg catgaggctg ccagcacagt cgaagtgtgg atgattccta
     3661 ccgacgaagg ccgcgtggcg gcgcaggccg ccgctgccct gcttcaggcc caagcctgat
     3721 actcaaatag agacgctatc gggctgctcc tgcccgcctg cgccctcccc ctttagcgct
     3781 ttttggctgt gatggtaggc agagcttcca gtgcgctccg gaacgcagcc tccgacatag
     3841 ccgctagcgc cttgacaccg gcgcgcacca gctcactctt cttgactggg tgggcgaatg
     3901 tttcggcgcg ccgtttcagt tcctccaaaa cggcgtactc ggtcttcggg atactgaatc
     3961 ggtcacggac cagtttcgct tttttaacct taacgacctt ttcggccttg agtttggatt
     4021 tgggtccggc caacaccacc gacttgggtt tttccgcgac ttgcgtcgac ttgccggttc
     4081 gactggctgg cgatttgcgc ggggctgctg ttgtcgggga tttttttgcc ccggtggcag
     4141 cagtctttcc tgatggtttc tgggcagtgg ttgccatacg ttatttcctt ttgttaaaaa
     4201 acaataaagt cta
//
LOCUS       DAIDFG010000275         4188 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_275_length_4188_cov_19.220905, whole genome shotgun sequence.
ACCESSION   DAIDFG010000275 DAIDFG010000000
VERSION     DAIDFG010000275.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_275_length_4188_cov_19.220905"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          809 a         1341 c         1362 g          676 t
ORIGIN      
        1 tgccccgacg tggccggccg cggccgcagc gactggctcc aggaccccat gggctaccag
       61 attccgcagt acgccgccga catgatggca ctgctggcgc acctccatca gcaagcgccg
      121 gtcacgacgc tggactgggt tggcaccagc atgggtggcc tgatcggcat ggccattgcc
      181 ggccagcccg agctgcccct gccggcgccg gtgcgcaagc tggtcctcaa cgacgtgggg
      241 ccggccatcg agtggcaggc catcacgcgg attgcggcct acctcggcaa agccggccac
      301 ttcgcctcgc tgcagcacgc ggccgacgcg atgtgggcga tctcgaccag ctttggcccg
      361 cacacgcccg agcaatggct ggcgctgtcg cgccacatgg tcaagcctgt gcccggtgcc
      421 accgatggca gcgtgatgct gcattacgac ccggcgattg cggtgccggt ccgggcgacg
      481 accgaagctt cggcgcgcga cggtgaagcc ttgttgtggc aactttttga caacattcgg
      541 gcgccgacac tggtcgtgcg cggcgcacaa tcggatctgc tcagccgcgt cacggcgcag
      601 gccatgatgc agcgcggtcc ccgggcccgc ctggtggagt ttgaaggggt cgggcatgca
      661 ccgaccctgg tggcgatgga tcaggtcagc acggtgggcg attttcttct tgtctgaaat
      721 tttttattga aaagctgcct gtgacgaaac atgccggggc cgggccgggc gccccgccca
      781 aatcgccgtt gaagccccag acggccgcgg cgcccatgac gcccatgaca ccgctaccca
      841 atgcgtccat cgtgacggcc acggcggaca gcctgccgga ccagccgcat gcgctggccc
      901 gcgccagggc ctttgccgag ccgctgatcg ccagtgaaac gctggacacc ggtgaaaaca
      961 tcctggccca tgcggatgct gtcgcagcca ttctcaaggc cattggcggc tccgaggcca
     1021 tgcaggccgc cacctacctc gttcacgcct gcgcgcacct gaacaagccg caggaggtga
     1081 ttgccaaggc tttcggcgtc aacttcgccg ccctggccat ggagaccacc aagctcgtcc
     1141 atgtgcagaa gcaggcccgc gccgccgatg ccagggcgca gctggaagac ccggcagcgc
     1201 agactgaaaa cgtgcgcaag atgctgctgg cgttctcgcg cgatctgcgg gtggtcatgc
     1261 tgcgcctcgc gtcccggctg cagacgctgc gtcactttgc ggccatacgg caaccatgcc
     1321 cgctggccgt ggcgcgcgag tcgctcaacg tttttgcacc gctggccaac cggctgggca
     1381 tctggcagat caagtgggaa atggaagacc tggccttccg ctttctggag ccggacactt
     1441 acaaaagcat tgcccgcctg ctcgatgaaa aacgcgccgg gcgcgaggtg ttcatggagg
     1501 cgctgcgcgc ccagctggaa cgcgacctga acagcaacgg cattgccgcg ctggtgcagg
     1561 gccggcccaa acacatctac agcatcgtca agaagatgcg cggcaagggt ctcgattttg
     1621 accaggtctt cgacatccgc gccctgcgtg tgatcgccgc cgacgtcaag ggctgctacg
     1681 cggcactcgg gtttgtgcac tcgcatttca cgccggtgga gggtgaattc gacgattaca
     1741 tcgccaagcc caaatccaac ggctaccagt cgctgcacac ggtggtgcgc gatgcgcagc
     1801 aacgggcggt agagatccag atccgcacgc aggccatgca cgaccatgcc gagcatggcg
     1861 tggccgccca ctgggcctac aaggaggccg gcgccaaggg ttacagcggc gtgtcggcca
     1921 gcagcgagta cgacgcaaaa attgccgtgt tgcggcagtt gctggcctgg gagcgtgacc
     1981 tgtcggggcc ggtggcggcc agggcgcgcg acgccaacca gggcctgttc gaagaccgca
     2041 tctatgtcct gacgccggac gcggccgttg tcgaactgcc gcagggcgcg acggcgctcg
     2101 actttgccta cagcgtgcac accagcctgg gccaccgctg ccgcggcgca cgcatcgacg
     2161 gcgccatggt gccgctgaac acgccgctgc agaacgggca gacggtggag gtcgtcacca
     2221 cgaaggaggg cggcccgtcg cgcgactggc tcaaccccga actcggcttt ctggccagcc
     2281 accgcgcgcg gtcgaaagtg cgtgcctggt tcaatgccca ggccatggcc cagacggtgg
     2341 cgcgcggacg cgaggcggtg gaaaagctgc tgcagcgcga gggcaagacc gcgatgaaac
     2401 tcgaggatgt cgcgacgcag ctgggcttca aaagcgccga ggatctgttt gaagtggtgg
     2461 gcaaggacga gctgtcgctg cgcaccatcg agaacatgct caagccgccc gagccgccac
     2521 ccacgcagga cgactacgtg ctggccaaaa aagcccgcca gcatgacaag tcgtccgagc
     2581 gcgcacgcaa gggcggagtg ctggtggtcg gcatcgactc gctgctcacg cagctggcca
     2641 ggtgctgcaa gccggcaccg cccgatgcga ttctcggttt tgtgaccaag ggcaagggcg
     2701 tgagcatcca ccgcagcgat tgcagcaact tcaggaacat ggccagcggc agccccgacc
     2761 gcgtgatcga agtgcaatgg agccagccca aaagcagcga gggctcggtg tacccggtgg
     2821 atgtggccgt ggaagcggcg gaccggcagg gcctgctgcg cgatatttcg gaagtgtttg
     2881 cgcgggaaaa aatgaacgtc atcggcgtgc agaccacctc ggtcaaggac aaccgcggcg
     2941 gcacggcctg gatgactttc acggtggagg tggccaactc gggccggctc aaccaggtgc
     3001 tggccatcgt cgccgaggtc aacggcgtgc gttcggcgcg caggcgctga tttgtcggca
     3061 aaaaaatcgg cgagggcgca aaaggccggg tttgccctgc tacaatgacg aggtcgaaca
     3121 gtccttaggc gcgtagctca gctggttaga gcaccacctt gacatggtgg gggtcgatgg
     3181 ttcgagtcca atcgcgccta ccaatcacat cgggtcggca acagccggcc tcaaatgggg
     3241 gcaaggcccc cctggaggtt gcgaaatgtc accgcgaact agtttgactg tcactggata
     3301 aatcccggcg cacagcagtt cgcaatcgtt ttgcaattct tctcaagcag catcaaaaaa
     3361 gcgcggccgg tccgcgcttt ttcattttcc ggagtttgaa acatggtcaa cgttacgcta
     3421 cccgatggtt cggtccgcca gttcgaatct gctgtcaccg tgtccgaagt cgctgccagc
     3481 atcggcgccg gcctcgccaa ggcggcgctc gccggcaagg tggacggcaa gctggtcgac
     3541 accagccacc gcatcgaagc cgacgccacg ctcgccatcg tcaccgcccg agacgccgac
     3601 gcgctcgacc tcatccgtca cgacgccgcg cacgtcatgg cgcaggcggt gcaggagctg
     3661 tatccgggga cgcaggtgac catcggcccg gccattgaag acggctttta ttacgacttc
     3721 gcgcgcgaga cgccgtttac gccggaagac ctcgttgcca tcgaaaagcg catggacgag
     3781 atcgtcaaac gcgatctgcc gattgtgcgc gaggtgtggg aacgcgaggc ggcgaaaaag
     3841 accttcgccg ccatgggcga ggactacaag gtccagatca tcgacgacat catcgcgccc
     3901 ggcgaggagc tgtcggttta ccgacagggc gactggttcg acatgtgtcg cggcccgcat
     3961 ttaccctcaa ccggcaaact gcccaaagcc tacaagctga tgaagcttgc cggtgcgtac
     4021 tggcgcggcg actcgaaaaa cccgatgctg caacgcatct acggcacggc gtgggcgaag
     4081 aaggaagacc tcgaagccta tctttttcgg ctcgaagaag ccgagaagcg tgaccaccgc
     4141 aaactttgta aacagcttga tctcttgcac atgcaggaag aagcgccg
//
LOCUS       DAIDFG010000276         4177 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_276_length_4177_cov_19.334061, whole genome shotgun sequence.
ACCESSION   DAIDFG010000276 DAIDFG010000000
VERSION     DAIDFG010000276.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_276_length_4177_cov_19.334061"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          675 a         1464 c         1372 g          666 t
ORIGIN      
        1 gcgccgagca gaccgccgca gcgcgcgcca tgaccttgcc gcccgcatga gtttttcgcc
       61 gattttcccg ggcaccgtcg atgccgcggg ccagcccgcg accctgggcg tgatgggtgg
      121 cggccagctc ggccgcatgt ttgtgcatgc ggcgcagcgg ctcgggtatt tcaccgcggt
      181 gctcgacccc gacccgacca gcccggccgg ccgcgtcagc cactaccaca tccagaccgc
      241 ttaccgggac gagaacggcc tggcgcagct ggcttcactg tgcgccgcca tcaccaccga
      301 atttgaaaac gttcccgccg acgcgctgca aaccctggcc gtggaccggc cggtggcgcc
      361 cggcgcggcg gccgtcgcca ttgcgcaaaa ccgcatcgag gaaaaagccc acttcaccgc
      421 cagcgccggc atggcgggtg tgacctgcgc gccttatgcc gtgatcgaaa cgtcggcgca
      481 gctcgccgcc gtgccggccg acttgctgcc cggcatcctg aaaacggcac gcatgggtta
      541 tgacggcaag ggccaggtgc gcgtcaaaac cgctgctgaa cttgccgccg cctgggccga
      601 tttgaagcag gtgccctgcg tgctggaaaa gctgctgccg ctgaaggccg aatgttcggt
      661 catcgtcgcg cgcggctggg acggcaccat caccggtttt gcgccgcagt acaacgtgca
      721 cgttgatggc atcctcgccg ttaccgcggc ttatgaggga aacatgcctg cagcccttgc
      781 acagcgggcg cgagcagcta ctgaatccat agctaatcac atcgcttatg tgggcgtgtt
      841 gtgcgtggag ttttttgtga tcgacgacgg cagtgccgat ggcgcgctgg tggtcaacga
      901 aatggcgccg cggccgcaca acagcggcca ctacacgatg gacgcctgcg acgtttcgca
      961 gttcgacctg caggtccatg ccatggccgg cttgccgctg ccgcagccgc gccagcattc
     1021 gccggccatc atgctcaacc tgctcggtga cctgtggttt gacgccgccg accagttgcg
     1081 cacgcccgac tggcaggccg tgctggcctt gccgggcacg catttgcacc tgtacggcaa
     1141 gctcgaagcg cgcgccggcc gcaagatggg ccacctcacc gtcaccggcg ccgacgtggc
     1201 cagcgtcacc gcggtcgcac gccaggccgc gcagctgctg gggctgcccg gcctggacgc
     1261 cttgcgatga ttcggggcgg caccgacccg gcggccattc tggaagccgc acgcgtgatc
     1321 cgcgccggcg ggctggtcgg ttttcccaca gagacggtgt atggcctggg cgccgatgcg
     1381 gccagtgaca ccgccgtggc cggcatcttc gccgccaagg gccggcccag cgatcacccg
     1441 ctgatcgtgc atgtggcgga tgccgcgcag gtgagcgact acgcgagcag cgtgccgccg
     1501 tttgccgcac gcctgatgca ggccttctgg cccgggccgc tgaccgtgat cctgccgcgc
     1561 aagccgggcg tggccgccgc cgccgccggc gggcaggact ccatcggcct gcgctgtccc
     1621 gcgcacccgg tagcactggc ctttctgaag gcctgcgcca ccggtgtggc cgggcccagc
     1681 gccaaccgtt ttggccgcgt cagcccgacc accgcccggc atgttgctga agaattcgac
     1741 gacacgctgc tggtgctcga cggcggaccc tgtgaggtcg gcatcgagtc cagcatcgtg
     1801 gactgcacgc gcggccgtcc ggtgctgctg cgccccggtt tgctcacacg cgcgcagctc
     1861 gaagccgcct gtggcgagcc ggtgttcgac aaggacgatt ggcagcagga agaggcagca
     1921 ctcggagaag caccgcgcgc atccggcacg ctggcatcgc actacgcgcc caacgcccgg
     1981 gtgcgcctga tggacgccag cgcgatccag accgcactcg acgtactcgg cgcggaggcc
     2041 gcgcacatcg ccgtgtatgc ccgcagcatc gtgcgcatca agtcggcgca ggtgctgtac
     2101 cgccgcatgc ccgacgatgc cgccgccacc gcgcagcagt tgttcgccgt gatgcgcgac
     2161 ttcgatagcc agggcgtcaa gctgatctgg attgaaaacc tgccggccga cccggcatgg
     2221 gatggtgtgc gcgaccggct gcagcgggcc gccgcgagtt gaggcgcgtt gaagtgagca
     2281 aggctgttcc atgtccgcat cgcgaaacct caaaacccgc ctggcctggt ttctggcgtg
     2341 cctgtcggca ggcaccgctg ccggcatggc gggtgcctgg ctcaccggca acccgtattg
     2401 gttcctcgcc attccggcgg cgctggctgt cgggtggctc ttcgtggcca acccggacga
     2461 gtgcaccccg ccctccacgc accgtgccgg cagggaaccg gacgacgaag gtgccccctg
     2521 agccggcgcc ggcttcccgt cgccttcgcg agcccgcggc ttgacgcccg ggccccgcac
     2581 catgcatagt gacccattca cccgctgtta tccagggaca ccgatgacat tctcactctc
     2641 aacaccgcgc acccatcgcc ccaacacagc gacccctcca gcgcaaggcc tgtcatgacc
     2701 caacctcccg gacccgtcgc cgtgagcgcc gtgcctgccg tttcggtcct gctgcagcaa
     2761 ctgcgtgacg gcagcgaccg cgccgcggcc gagcaggaaa tcctgcgcat cacggcccat
     2821 ctggaggagc agctgcgcca gcgcggcgcc gaggtggccg ccgccaacaa ggaactcgaa
     2881 aacttttcct attcggtctc gcacgacctg cgcgcgccgt tgagcacgat cgacggtttc
     2941 agccggctgc tcgagggcgc tgtcgccaag ggcaatgcgg aacgcagcac gcactacctg
     3001 cagcgcatac gcctgggcgt gggcaacatc aatgagctga tcgccgcgct gctggccgtc
     3061 tcgcgcctgc tgcgcgcacc gctggcgccg caggcggtgg acctgggcgc gctggcccag
     3121 gccgcgtttg ccgcgctgca ggaagccgac cccgcgcggg tagccgacat tacggtgcag
     3181 ccggcacttg ccgcgcacgg tgatccggcg atgctcaagc agctggtcga tcacctggcc
     3241 ggcaacgcgt ggaagttcac ctcgaaaaag gaacgcgccg acatcgtggt gggcagccaa
     3301 tcggggcagg acggtgaaac cgtttacttc ttcaaggaca acggcgcggg gttcgacatg
     3361 gcctacgccg agaaactttt cgggattttc cagcgcatgc atgtgagttc ggatttcccc
     3421 ggcatcggcg ccgggctgag cacggtgcag cgcattgtga tgcggcatgg cgggcgaatc
     3481 tgggcggagg ccgcgcctga gcaaggcgcg acgatctact tcaccttgcc aggggcctga
     3541 tcgccgctgt tgcgacaggg atgcatgtgc gcatgcatca cgctccgatc ccgtttcacc
     3601 caggacagta ggcgcaactc cccactccca ctcccacgcc gggcaacccc ccgcttgctg
     3661 gcacaccaca acagccaaac caacgcaggt ggctggcaaa catggatccc ggatcaagtc
     3721 cgggatgaca acacctcagg ccgcttcctt gtagaagtag tcgcgtttga tgccgcgcgg
     3781 cgccagcggc tcgaccgcct ggtgaatcgc cccgatgtgc tccggcgtcg tgccgcagca
     3841 accacccacg atgttgacca ggccttcgtc ggcaaactcg cgcagcaggc gcgaagtcac
     3901 ctcgggtgtc tcgtcaaacc cggtgtcgct catcgggttg ggcaggccgg cattggggta
     3961 gcaactgatg aaggtgtcgc cggccacacg cgccagttcc tggatgtagg ggcgcatcag
     4021 cgtcgcaccg agcgcgcagt tcaggcccac agccagcggc cgcgcatggc ggatgctgtg
     4081 ccagaaggcg gtgaccgtct ggccgctcag gatgcggccc gaggcatcgg tgaccgtgcc
     4141 gctgatgatc aggggcaggc gttcgccgga gttctcg
//
LOCUS       DAIDFG010000277         4153 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_277_length_4153_cov_17.581259, whole genome shotgun sequence.
ACCESSION   DAIDFG010000277 DAIDFG010000000
VERSION     DAIDFG010000277.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_277_length_4153_cov_17.581259"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          788 a         1287 c         1313 g          765 t
ORIGIN      
        1 gagaccagcg cctcggtgtg gtcgatgacg atggccccgc ccgagggcag gttgacctgg
       61 cgcgagaacg ccgattcgat ctggtgttcg atctggaagc gcgagaacag cggcacgtcg
      121 tcgcggtaca acttcacctt gccgacattg gccggcatca cgttttccat gaacgcgcgg
      181 gcctgttcgt acacgtcttc ggtgtcgatg aggatttcgc cgatgtcagc cgagaagtga
      241 tcgcggatgg cgcgggtgat caggttggat tcgaggtaga taagttctgg cgcgcgaatc
      301 tgcttggccg cgccgtcgat cgcttcccag atgcgcagca gatagctgag gtcccactgg
      361 aattcctcgg cactgcggcc gatgccggcg gtgcgggcga tgatgctcga acctgccggg
      421 tattccagct ggtccatgtt ttccttcagc tcggcccggt cttcgccttc gatgcggcgg
      481 ctgacgccac cgccgcgcgg gttgttgggc atcagcacga cataacggcc ggccagcgag
      541 acaaaggtgg tcagggcggc gcccttgttg ccgcgttctt ctttttcgac ctggaccagg
      601 atttcctggc cttcacggat cacgtcctgg atgcgcgcct ggctgaccga cacgccctgg
      661 gcgaagtatt gcttggagat ttccttgaac ggcaaaaagc cgtggcggtc ttcaccgtag
      721 tcgacaaaac aggcttcgag cgaaggctcg acacgtgtca cgacggcctt gtagatgttg
      781 cccttgcgct gttcgcgccc ttcaatttcg atttcgtagt cgaggagttt ttgtccgtcg
      841 acaatggcca ggcggcgttc ttccgactgg gtggcattga ccagcatgcg tttcatgggg
      901 tttccttcgt tcttctgttc tcaaacatca ctgcccgaaa aacgggcaaa cgacgccggg
      961 agcagacgtt gtgcgaacgt ggggaattgc cggagagctt ttgacgaagc tacggcgact
     1021 gaaaatcagt cgcagagcag tctgggcgta ggcgcgtgga tggggcgagt cggggtctga
     1081 caggtgtttg ctacaaaaaa agtagctgct tgcgcccgca ggacaagggc tgacggctga
     1141 ttaatgcctg aaagcggggc cagaaggcgc cgcaggcata aaaaaatcag tgctgccagt
     1201 gatgtcaaga ggctacccat gtaaagacca gatttcacct ggatccgtgt ccagcccgcg
     1261 gggctgcaca catcgtattc cgtagttgta ttcgcaaaac gtcgactcgt ctgcacaaca
     1321 tgttcctgcc gcggggtgcc tggcaccgtg cgcttgcggg catttgctgc cagtggcgtc
     1381 gaccttccag caaggctgtt gcagtgggtt gatgggtgtg tggactaaac tcccagacaa
     1441 atcaaccact tacagcttgt cgctagtgaa acacattata ggcgcaggtt caggtaaaac
     1501 acgccgcccg ggtaaacccc tgacggcagc gctgcctgac cgaccccgat cccccccgcc
     1561 tttgcaagca ccgccggaac tgcccgcaaa agcgcccccc caggccacgc tggtgacagt
     1621 ggacgctgac tttgcgggcc agcgcctgga caacttcctg atccgccagc tcaagggcgt
     1681 gcccaagacc cacgtctacc gcatcatccg tagcggcgaa gtgcgcatca acaagggcag
     1741 ggcgcaggcc gacacccgca tcgaggccgg cgacatcgtg cgcctgccgc cggtgcgcac
     1801 ctcggagcgg gccgacgaga aggcccgggc catggccgtc gaggtggcgc gtcacggcgc
     1861 tgcgtccacc acaggtggtt acgcgccgtc acgggagttc cccatcctgt ttgaagacga
     1921 gcacctgctg gccatagaca aaccggctgg cgtggccgtg catggcggca gcggtgtggc
     1981 ttttggcgtg atcgagcagc tgcgcatggc gcggcccgag gcgaggtttc tggaactggt
     2041 ccaccggctg gaccgtgaga ccagcggcat cctgctggtc gccaaaaagc gaatggcgct
     2101 gaaaaacctg caggagcagt ttcgcgagcg cgagaccgac aagatttacc tcgcgctcgt
     2161 cagcggcgcc tggccggtca ataaaaaagt gctggacgcc tccctgcaga aatatttgct
     2221 gcccgacggc gagcgtcgcg tgcgggtggt ggacaaggac catccggacg ccatgcgtgc
     2281 ggtgaccctg gtcaaggtga agtcggcgct ggctgcggcc gggcgcgatt tcacgcttct
     2341 cgaagtgacc atcaagaccg gtcgcacgca ccagatccgg gtgcacctgg ccagtgccgg
     2401 ccacccgatt gccggcgacg acaaatacgg cgacttcgaa ctgaacaaag ccttgcaaaa
     2461 agaagccggt ggcgtcgctc tcaaacgcat gtttcttcat gcctggcggt taaagttcac
     2521 ccatccgtcc agcggcaaac gtattgacct gctggccgcg ctcccccccg atctcgagag
     2581 tttcacgatc catgccacgc ccgtcctccc cgctcaactt tgacctgatt gccttcgact
     2641 gggacggcac gctgttcgat tcgacccaaa tcatcgtgcg ctgcatccag gcggcggtgc
     2701 gtgacgtggg cggcaccgtg ccgaccaacg aagccgcggg ttatgtgatc ggcctggggc
     2761 tgatgcaggc gctggcccat gcggcgcccg atgtcccgcc cgagaagtac ccggagctcg
     2821 gcgcgcgtta ccgccaccac tacattggcc accagaacga catcagcctg tttgacggcg
     2881 tgctgccgtt gctggccgcg ctcagggcgc gcggccacct cctgagcgtg gcgaccggca
     2941 agagccggca cggcctcgac gaagcgctgc aggcggtcga actcaagggc cggtttgacg
     3001 gctcgcgcac cgccgatgaa accgccggca agccgcaccc gcgcatgttg catgagctga
     3061 tggaagagtt tggcgtgagt ccggagcgta cgctgatgat tggcgacacc acccacgacc
     3121 tgcagatggc gctcaacgcc ggctgtgcca gcgtcggggt cagttacgga gcgcacgaaa
     3181 gtgcggcctt tgacgccctg cagccacgtt ttgttgccca ctccgtgcag gacctgcacg
     3241 actggctggc ccgccatgcc tgatccggac ctggccctga tcccgctgtg ccgcatcggt
     3301 gaactgttgg agaggggcga ggccgtgcct ttcgacgtga actacgcagg ccagacctgc
     3361 cgcgcctttg ccgtgcgctt cgaaggacgg ccccacgcct acctgaaccg ctgcagccat
     3421 gtggcgatgg agctggactg gcagcccaac cgggtcttcg acgacagcgg ccagtggctg
     3481 ctgtgcgcca gccacggcgc ggcctaccga cccgacacgg gcgagtgcag cggcggtccc
     3541 tgccagggcg ggctggtcaa gattgatatt tcagaaggcg agggccttgt tttctggcac
     3601 acgtcctaca aacttaaaac attggagttc taaactaccc gtatgaatga ttcaaaccgc
     3661 accgaaccgt ccgacaaccc ggattttgat ccaaacagat cgtcagccca gcaaacacgg
     3721 gcgcaggcag ctcctgaaaa cgtagcaacc agcaagtccc tgagcaccga gccgggctgg
     3781 gagcgcgcga cgctggagaa gctggctttt gccgccctca acgagcagaa ggccacgcgc
     3841 cgctggaaga cctttgtgcg cctgtcctgg ctggcctttt ttgtgttcct ggtgtggttt
     3901 gccatgcacc gcggcgcggc caccaccaac gtcagcgcgc cgcacaccgc ggtggtggaa
     3961 atcaagggtg agatcgccga cggcgccgac gcgagcgccg agttcgtcaa tgccgcgctg
     4021 cgcgccgcgt ttgaagacga gggtgcccag gcggtggtac tgctgatcaa ttcacccggc
     4081 ggcagcccgg tgcaggccgg catgatgaat gacgagatcg tgcgcctcaa ggccaagtac
     4141 aaaaaacccg ttt
//
LOCUS       DAIDFG010000278         4152 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_278_length_4152_cov_19.612399, whole genome shotgun sequence.
ACCESSION   DAIDFG010000278 DAIDFG010000000
VERSION     DAIDFG010000278.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_278_length_4152_cov_19.612399"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          708 a         1386 c         1318 g          740 t
ORIGIN      
        1 ggcgaaattt atgtcgatct ggccctgccg cccagcgagc ccgtcagcga ccactgcggc
       61 agctgcagcg cctgcatcac gatttgcccg acgcaggcca ttgtggcgcc gctcaagctc
      121 gatgcacggc gttgcatctc ttacctgacg attgaacatg cgggctcgat tccgctggaa
      181 ctgcggccgc tgatcgccaa ccgcatctac ggctgcgacg attgccagct ggtctgcccc
      241 tggaacaagt ttgcgcagcg cagtgccttg cccgacttcg acgagcgcca gggcctgacc
      301 ggccagcaac tgacgacgct gttcgcctgg accgaagaag agtttctgcg ctacaccgaa
      361 ggcagcgcca tccgccgcat cggccacgag cgctggctgc gcaatatcgc cgtggcgctc
      421 ggcaatgccc tgcgcgccgg tgcggacgac agcctgcgca gcgccttgct ggcgcggcgc
      481 gaacatggca gcgaggtggt cagggaacat gtggcgtggg cgcttgatca ggcgcccctc
      541 ctggagttgt ccataaaata ggcgtccggc ccttgacggg cgggcgcagt cagctattca
      601 ttttgtagca gtataggcgg ggccaagccc catcgtccag ccccgccggc aaagcccgtc
      661 agcggcgctc cttataatca cgctgtgtcc aacactccgt cctgtcgccc tctgtgggcc
      721 cgcttcgccg cgtggctggg gtttctggcc gtcttttcgg cgctgctggc gcccgtgtcc
      781 atgctggccg aggacatgcg gactggcaag ctgggcggtc tttgcaccgt ggccagcggc
      841 gcatcgtccg ctgcggccgg ggacttgtcg ggcgatgacc agtcttcacc ggctgccgcg
      901 cattgcgacc tctgcggctc gaccgggccc ggtttgccgc cgtcctggca ggccggcgtt
      961 tttcccgcac ccatcaccgc gatggttgcc gtcttccagg atgccgtccg cccggcgtct
     1021 ttgccggggc tgcccttcag ccgcggcccg cctgcacttt gaactgaagc gcctctgacg
     1081 aggttttccg cgcgtgggcc tgaccgcccg cgtgcttgtg ttcatttcaa tgtgtgggtt
     1141 ccatgaaatt ttcatcagct atcaaaaaga tagcgttatg cggagccgtg acgggcgctg
     1201 cgcccgcttt tcctcatatt tccctggccg agggtcaggc cccggccgga cagcaccatc
     1261 actgacactg accacttttt ttcgactcat cctcattttt ttaacgaaag ctattcatga
     1321 aaacaaaagt tattgccgcc ctgttggtac tttccgccgg cgctgtgttt gcccagacgg
     1381 tcgaggtcaa ggacgcctgg gtgcgcgccg ccgtgcccgg ccagtcgggc accggcgcct
     1441 tcatgaacat cacggccaaa gacggcgcaa agctggtggg cgtgtcgtcc ccggttgccg
     1501 gcgtggcgga agtccatgaa atgaagatgg acaacgacgt catgcgcatg cgcgccatcc
     1561 ccgcgctcga cttgcccgcc ggcaaaaccg tggcgctcaa gcccggcggt taccacgtga
     1621 tgctgatgga gctgaagcag gccctgccca agggcagcac ggtgccgctg accctgatac
     1681 tgcgggacgc caaagggcag gaaagcaagg tggagctgaa gttgccggtg gccgcttcca
     1741 tgccgggtgc gggcatgccg gcatcgggca tgcccggcgc catgcacccc aagcactgac
     1801 tatttggatt tgccgcccag acggcggtag acggtggcgc ggctgatgcc gagcgcctgc
     1861 gccgctttca tgacgttgcc ccgggcttcg ttgaccgcct tgcggatcag cgccgtttcg
     1921 acgtccttga gcggaagggc cggccccgcc gccgcccggc cgctgaaccg gcccggtgcg
     1981 gccgcgcggc cgcgcagcag cggcatgacg tgcaggcgca gtccggacca caagggcact
     2041 tccatggctt gggggttgcc cgcggcgtcg ctcctggcgg cgtcgaacag catctcgtaa
     2101 ggcacggcaa acagctcgtt gcagtgaacc gccgccgggc tgccggcctg cagcgccggc
     2161 accatctgcc gcgccgccgg gttggccccc agcacccagc cgtccgggtc caggctgacc
     2221 agcccgtcgc tgtcttctcc caggctgtgg ctggaccagt tcagccgtat cagcagatcg
     2281 tgcggccggc tctggatcag ggcgttttcg atgctgcggg ccgactgcac caccaggtgt
     2341 ttgagctcgg ggcgctcgac cgtgtccaca ccggtcaggt ccagcatgcc ggcgcagcgg
     2401 ccgtccggcc cgaacagcgg cgcaccggcg cagctgtagg ccgagttggc attgaaaaaa
     2461 tgctcgccgc ggtgcagcca gacgggctgc agctcggtca gcgccgcacc gatggcggtg
     2521 gtgcccacac tttgttcgga caggtccacg ccgatgcgcg tgatcaggtc ggcgcgccgg
     2581 tcacgccggt cgatggcgcc gttgacatcg accaccacgc cctgcgcgtt ggtcaggatg
     2641 gcgaaataac gcgtgctggc aatcgccgcg ccaagttttt cgagcaccgg ccgcgccgcc
     2701 tgcactagca cgtggttggc ttcttcggtg cggcgcagcg cctgggccga cagcacgtcg
     2761 aaaccgacct cctgctcggg ttttttgccg ctggccaggc agcgctgcca ggagcgctca
     2821 atccacgatt cggccagcag gccgggctgg ctccggccgt cccgcagcac ggcctggcgc
     2881 gcttgcgcga tctgttgcag gcgctgttcg ggggaggagg gaaggacggt cagcatgggt
     2941 ggtcaatgct cttgaaggcg gctgtctgcg ggaggatgcc cgcggtggct gcagcgtagc
     3001 agacctcgcg ccgacgcgct gaaggcctgt cagcaactgt tccattttga gaatttaacc
     3061 gggctttggc aagctcctag ggtttaacct aggcccctgc ggggtcgcca cttcaacaat
     3121 gacccttgtt ccgtccgtag ccaaacccgt cgtcaaaaaa tagccaactg gagacaagcc
     3181 catgcaagtt caattcaaag tcaacggaaa agccaaaagc gttgaaaccc cgcccaacac
     3241 cctgctggtg caggtgctgc gcgaacagct gaagatgacc ggcacccacg tcggctgtga
     3301 caccgcccag tgcggcgcct gcacggtcat catgaacggt cgcgcggtga agtcctgcaa
     3361 catcctggcc gcccaggctg agggcagcga gatcaccacc atcgagggcc tggcagccgc
     3421 cgacggcacc atgcacccga tgcaggccgc cttcaaggaa tgccacggcc tgcaatgcgg
     3481 cttctgcacg acaggcatgg tcatgagcgc ggtggacctg tgcaagcacc accccaaggc
     3541 cagcgaaggc gaaatccgcg agctgctcga gggcaacatc tgccgatgca ccggttacca
     3601 gaacatcgtc aaggcggtgc agcagggcgc tgccgccatg gcgacctgaa ccaccgcgct
     3661 gacctctttt tccggcgccg ccatcgggcg gccgctggca accctgtcaa acagtttttt
     3721 tccaaatttt caggagtaca cacatgggtg catccgattt cgtcaacctg ccaaacattg
     3781 gcacctccgt ccgccgcaag gaagattacc gctttctcac cggcggcggg caatacaccg
     3841 atgacatcac gctggccaac cagagttatg cggtgttcgt gcgttcgccg cacgcccacg
     3901 ccaggatcaa cagcatcaac accgccgccg ccaaggccgc gcccggtgtg ctggacgtgt
     3961 ttgtcggcga ggatgtggcg gccgacaaga tcaatggcct gccttgcggc tggctgatca
     4021 cctctacaga cggcaacccg atgaaggagc cgccgcaccc catcctggcc cagggcaagg
     4081 tgcgttatgt gggcgaccat gtcgccatgg tggtggccga aacgctggag caggccaaaa
     4141 acgccgccga gc
//
LOCUS       DAIDFG010000279         4129 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_279_length_4129_cov_19.097693, whole genome shotgun sequence.
ACCESSION   DAIDFG010000279 DAIDFG010000000
VERSION     DAIDFG010000279.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_279_length_4129_cov_19.097693"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          624 a         1328 c         1394 g          783 t
ORIGIN      
        1 agcgcgaagt gtttggctgg gccatgtacg atttcgccaa ctcgggttac accacggttg
       61 tgatcaccgc ggtgtttgcc gcctatttcg tgggaggcat tgcgcagaag gctgagtggg
      121 ccacgttcgc ctggacggcg gcgctcagcg tgtcctatgc gattgtcatg ctcaccatgc
      181 caagcctggg cgcctatgcc gacctgcgcg ctgccaagaa acgcctgctg atgctgacca
      241 cggcgggctg cgtgctcacc acggctgcgc tcgccctggc gggccccagc agcgtggcgt
      301 ttgcgatggt gctgatcatc ctgtcgaaca ctttttttgc ctacggcgaa tcgctgaccg
      361 ccgcgtttct gcccgagctg gcccggcccg tcgcgctggg caaggtcagt ggctggggct
      421 ggagttttgg ttatttcggc ggcatgctgg ccctgggtat ctgcctgggc tatgttttgt
      481 gggcccagtc ccggggtatc ccggccgacc agtttgtgcc ggtcaccatg ctgatcacgg
      541 cggtaatcta cgggctggcg tcgctggtga cttttcgcct gctgaaggaa agggcccaac
      601 ccaaccctgc ggcattgcag cagggcggct tgaaggcctc tctggcgcag ctgcaccgga
      661 cctttcgcca agccaggcgt tacaaggact tcatgtggct gcttgcctgc gccgtgttct
      721 accagggtgg tgtggccgtg gcgattacgc tggccgccat ttacgccgag caggtgatcg
      781 ggtttcagca gcaggagacc atggtgctga tctttgccct caacttcgcg gccgccggcg
      841 gtgcgttcgt gtttgggtat ttccaggacc gcattggcca caagctggcg ctgggcatca
      901 cgctggtcgg ctggatcgcc acctgcgtga tcgccgccgc caccaccacc aagggtggat
      961 tctggtatgc agccgccatt gcgggtgtgt gcatgggttc cagccagtcg gcgggtcgcg
     1021 ccatggcggg gctgtttgcg cccaaaagac aattggcgga gttttatggc ctgtggacat
     1081 tcgctatccg tttggcgagc atcatcgggc cgctgagcta cggcgccatt acctgggcga
     1141 cgggcggcga ccagcgcaca gccattttgt ccaccgctgt cctgtttgcc atgggcttgc
     1201 tgttgctgct tccggtcaat atgcaacgcg ggcgcgaggc ggccacggcc gcagacgctg
     1261 gggctggctg atgccgcttt ggcagcacga gcggcatgag gcgctgcgca cgatgccatg
     1321 gcacgagcag cgggctcgcg acctgatcaa gcgcatcgtc agcgacacag aatcccacgc
     1381 cggcccttgg cccaatcacc cgctggacgg cgccgacccg gcaccgcgcc acgagctgta
     1441 cggcggcgct ggcggcgtga tttgggcgtt gcatcacctg cagcagcgcg gcgccgtgaa
     1501 gctggccaat gcgcttcagt ccgcgcaggc gctgcggttg ctggagctca accgcgctct
     1561 gatgggccgc agccaggggg ttttcggcgc ttacctgatg ggcgatacca gcctgcgcat
     1621 gctggcgttc gccatcgagc ctgacgaaaa gcaggccgcc gcgatcgcgc aactcgtcga
     1681 ggctaacgtc gatcacccgg ctcgcgaact gatgtggggc tcacccggca ccatgctagc
     1741 ggcgctgttc atgcagcacc gcaccggcaa agcgcgctgg acaagcctgt tccgccagac
     1801 cgcggagaaa ctcgcctcgc agctgctgtg gtcgcaggag gagggctgcc actactggga
     1861 ccaggacctg tacgggcaga catcaactta cttggacggt gtgcatggct tcgcggcaac
     1921 cgcctccgtg ctgctgcagg gccgcgacct attggccccg gagcaagccc gcgcctggga
     1981 ggaacgcatc gccgccacca tgggccgcgc tgccgaatgg gaagatggcc tcgccaactg
     2041 gcgcaaccag ctgacgcgcc cgcgcggcgg ccccatgctg atgcagtact gccatggcgc
     2101 accgggctat gtgatctgcc tggccggcat tcccggccac gcgcttgacg acaccctgct
     2161 cgcagctgga gaggcgatct ggcaggccgg tccattgtgc aagggctcga atctgtgcca
     2221 cggcacgggc ggcaacgggt atgcctttct caagctgcac cggcgctttg gtgggacgtg
     2281 ttggctggag cgggcgcgcg ccttcgccat gcattcgatc gtgcaggccg aggaggcgct
     2341 ggccgagcac ggtcaatggc gcttttcctt gtggaccggc gatctcggcc tggcgatcta
     2401 cctgctggat tgcatcgagg agcgggaccg cttccccacg ctcgactgct tcttcgacta
     2461 aaggcccagc caacgcgccg cggcagcctg cacggcccgg gcgtcgcccg tggctagcca
     2521 ctgcacgcgg ccgccgcctt gcgggtttaa caggcccgcg tcctgcagca ggcgccgggt
     2581 ctggtgggcc acaggctcgc cggtctcgat cagggccacc tccggtccgg ccaattcggc
     2641 aaaatgcggc gccgcgaatg ggtagtgggt gcaacccatc accagggtgt cgatctgacc
     2701 gttttgagag ccaaatcgat ctgcagatcc tatgtattct gagcagagtg ctcttatttc
     2761 tgtagcgtcg tcggcttcga tggcggccgc taggccggcg cacggctgca gcacgaactc
     2821 cgcttgaccg tgcagcgaag cgtgtagcgc cgcgaatttc gccgaggcca aggtggcacg
     2881 cgtggcgagc acagcgatgc gcttggtact gctacgtgcg gccgccggct tgagcgcggg
     2941 ttccacgcca acgaggggca ggcccgcatg ggcagcacgc agcaggtgca ctgcggcggc
     3001 cgttgcggtg ttgcaggcca cgaccagggc cttgatggca tgctcttgcc gcagttgccc
     3061 ggcgatcgcc agcgaccgct gcgccacgaa ggcgtcgtcg cgctcaccgt acggcgcatg
     3121 gccggtgtcg gcgaagtaga cgaagtcttc ctgcggcagg gcagtgcgca gcgccctgag
     3181 gatgctcagg ccgccgacgc cgctgtcgaa cacccctatg ggtgcgtcag gccgtagcaa
     3241 cagggattgt tttgaattca cccgtggcaa tcttcttctg ccattcggcc gggccggtga
     3301 tgtgggcgct ggtaccgccc gaatccaccg ccacggtcac cggcatgtcg atcacgtcga
     3361 actcgtaaat cgcttccatg ccgaggtcgg cgaaaccgac aacggtggcg ctcttgatgg
     3421 ccttgctgac caggtaggcg gcgccgccaa cggccatcag gtaggcgctt ttgtgtttct
     3481 tgatggcctc gatcgcgacc gggccgcgtt cggccttgcc gatcatggaa atcaggccgg
     3541 tctgcgccag catcatctcg gtgaagccgt ccatgcgggt ggccgtcgtc gggccggcgg
     3601 ggccgaccgc ctcgccggcg accgggtcca ccgggcccac gtaataaatc acgcggttgg
     3661 tgaagtccac tggcagcttc tcgcccctgg ccagcatgtc ggaaatccgc ttgtgcgcgg
     3721 cgtcgcggcc ggtcagcatc ttgccggaca gcagcagggt ctggccgggc ttccagctcg
     3781 cgacctcggc ctgggtcagt gtgtcgaggt tgaccttctg gctcttcacg tagtcaggcg
     3841 tccagttgac gttgggccag aggtccaggc tgggcgggtc cagatacacc gggccgctgc
     3901 cgttcagcac gaagtgcgca tggcgtgtgg cggcgcagtt ggggatcatc gccaccggct
     3961 tgctggcagc gtgggtcggg tacatctgga tcttgacgtc gagcaccgtg gtcaggccgc
     4021 ccagtccctg cgcgcctatg cccagcgcgt tgactttgtc gaacagttcg agccgcagcg
     4081 cctcgacctt ggtcagcgcc gcgcccgagg ccgccttggc ctgcagctc
//
LOCUS       DAIDFG010000280         4111 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_280_length_4111_cov_14.978550, whole genome shotgun sequence.
ACCESSION   DAIDFG010000280 DAIDFG010000000
VERSION     DAIDFG010000280.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_280_length_4111_cov_14.978550"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          691 a         1383 c         1265 g          772 t
ORIGIN      
        1 caggcctaga ttgtcaccgt tttgccggcc ctatccacgg gggtgccggc cccctacaat
       61 gcaggcatgc aacaccccct tcgctccctt ctgttcgccg ccacggctgt tctggcctgt
      121 gcccccgtgt tggcccagac agcggcgcct gccccggcag ctgcacctgc gcaagcactg
      181 ccgcccggca ccaaccccgc cagcagccgg cccgaaccgg ccatccagcg catccgcacg
      241 gaagacgccg gttcgcgcat cgacgaagtg cgtgtgggtg gcgagacaca aagcatcacc
      301 gtgcagccca aagccaatgt gccggcctac gaggtcctgc cttccgacgc caccaagggc
      361 ggcggcaccg caccatccac ctcgggcgcc ggcagcaccg gcaaacgcgt ctggaacgtg
      421 ctcaagttct agtttctccc cttcagtttc cgtttcaggc atggccgttt acaccgaagt
      481 ctcgtttgac gaggccgctc cccttttgca ttcgctcggt ctggggcagt tgaagaccct
      541 ggacccgtgc tcgggcggca tcgagaacac caactatttc gccgacaccg atcagggtcg
      601 ctacgtcctg acgctgttcg aacgcctggg tttcgagcag ttgccgtttt acctgtacct
      661 gatgcagcat ctggcgcagc gcggcattcc cgtgcctgag ccacatgcag ccatgacggc
      721 cggcgcaggc agctggcccg gcgacctcct gcacaagctc aagggcaaac ccgccgccgt
      781 ggtggaccgc ctgcgcggcc gcagcgaact ggcgcccaca cccgcccact gtgcggcggt
      841 gggcgacatg ctggcgcgca tgcacctggc ggggcaggac ttcgaccgcc agcaggccaa
      901 cctgcgcggc ctggcctggt ggaatgagac cgtgccggtg gtgctgccct acctcgaccc
      961 cgaacaaggc agcctgatcc gctcggaact cgcctaccag aaccatgtcg ccgcctcctc
     1021 ggcctggcag gcgctgccgc gcggcccggt gcatgccgac ctgtttcgcg acaacgtgat
     1081 gtttgacggc gaaggcagca gcctgcgcct gacaggcttt tttgattttt acttcgccgg
     1141 ctgggacgcc ctgctgtttg acgtcggcgt cagcctcaac gactggtgtg tggacctcgc
     1201 caccggtgaa cgcgacacag cacgcacctc ggccttcctg gcggcctacc aggccgtgcg
     1261 ccccctgaac gcccaggaac gcagcctgtt gccggccatg gagcgcgccg gcgccctgcg
     1321 tttctggatc tcgcggctgt gggactttta cctgccgcgc gaagccgcac tgttgcaggc
     1381 gcacgatccc ggccattttg aacgggtgct gcgccagcgc gtggcccatc ccggcggcct
     1441 gtcgcactga agcgggccgc catcacgcgc cggcgaaaag ttactggcgc gccttctttt
     1501 caagcaaact tgaccgctgg cgttaaattg ttcaccccat cgcccttttt ccacggttcc
     1561 ggtgaacccc gacggtccga acccctcaac atgcaaccca agcaatgaaa ctaaatatcg
     1621 ttcccgcgcg tactggcatc acatgggtca aactcggcat acaaaccttc ttcaagcagc
     1681 cgctggcgtt ggccggcctg tttttcatgt acatggcggc ggctacgctg gcatcgctga
     1741 ttcccttcgt tggcgtggtg ctggcgctgg ccatcgtgcc agcggccacc ctgggcctga
     1801 tggccgcgac gctggaggcc accaagggcc gttttccgat gcccgccatt ctggtcagcg
     1861 ccttccgcgc cggccagcag cgcgcccgcg ccatgatggt gctgggtttt ctgtatgcgg
     1921 ccgcctgcct gctgatcgtg tccgtggtac cgctgttttt cgatgtgccg gtcagccgcg
     1981 aaagcgccat ggcgcccgaa atgcaggggg ccatgctggc catcatggtg ctgtacctgc
     2041 cggtctccat cctgttctgg catgcacccg cgctggtgca ctggcacggc gtgacgccgg
     2101 tcaagagcct gtttttcagt gccgtcgttt gcttcaggaa tgccggcgcc tacgcgctgt
     2161 tcggcctgac ctggatggtg gtgctgatgc tcatcggcct ggccgtgagc ctgctggccg
     2221 gcctgctggc cagccccgag gccgcggccg cgctggtcat gccggccgcc ctgctgatgg
     2281 ccgcgatgtt ttcaagctcg ctgtatttca cctttcgcga cagttttgat gccaccccgc
     2341 ccgacagccc cgtcaataac gacaacaacc ccatcccagg agacacgcca tgacacagca
     2401 cctgctgcaa ggccggcaag aaaaggaaat cttgtggttc cagaggcgtg acgcgctcaa
     2461 ggccgccgcg gcctgggtgg ccatgggtgg cctgccggcc gcgatggcgc agcagcgcag
     2521 caacatcgtg cagctgaccg gcgacgccac cctcaacggc agccgcctga gcccggagca
     2581 cacgattcag accggcgacg agatccagac cggaccgggc gccaacctcg tgtttgcgat
     2641 cggcaactcg gccttccagg tgcgccagaa ctcgcgcctg tcggtggaac gtggcacgac
     2701 gctcaatgcg gtcagcctgc tgcgcctgct gaccggggcg gtggccagcg tctggggcaa
     2761 gggcgtgaac cgcgccatcg tcatgcctac gctgactgcg ggcatacgcg gcaccggggt
     2821 gtacgcagaa atttttgcag aacgggacaa tcggaactat ttttgcaatt gctacggcac
     2881 ggtcgatatg gatgcaggca accagaagct ggtttcgcaa gccgactacc accagtcttt
     2941 ctgggctgac gcggagccca aaaacggcgt cttcctgacg ccggcgcagg cgctgaatca
     3001 caccgacgaa gagctggagt tcctggcccg cctgattgat caacgcacca cctggcagat
     3061 tgccggcaga aaaggcaaca aggacggcag cggcaagatg acctactgag cggcagcgac
     3121 tctttccact gcgcgagccg gtatgggctc ctgcggtgct cgccgtacgc tggtacggtc
     3181 ccgctgctcg aagccacccg ggtccgctcg ctacggcatc ttcaaagccc ctgtggggcc
     3241 gtgactgctc ccgatggtgc tgctcagttc ttcgctttgt agtcagcgat ggatttgctc
     3301 agatcggcgt actcctcgca ggtcttgttg ccgcagatct tttccagctc ggccaggtgt
     3361 ttctcggctt ccagcgggcg cttttccatc aggtaggcct cgccgatgta ctcgtgcgca
     3421 cctttgtggc gagggtcaat tttcagcgcc gtgtagtagt gctcgaaggc cttggtgatg
     3481 tcgggcgtgg cgcgttttct gtagctgtag ccgagcaggt tgtgcacatc ggcgttgcgc
     3541 ggctcctccc gcgcggctgc actgagctcg gacatcgcgc ggctccagtc cttggcggca
     3601 atggctgtgc gggcagtgct gagccggtcg ctgaccgagg tgcgcggcat gttgggaatg
     3661 ctgcctgggg aatcgaccgc atgggccaaa gaagcgcagg ccgccagggc catcagtaaa
     3721 ggcttgaagt tcatcgcgaa ttctccggaa gtcggcgttg ttgccgattg tagtaacgcc
     3781 acaaaacgcc aactggatgc ggcacaaagt cacgactcgg cggcccgcat ggatgcggcc
     3841 gcaaaaagcc gcagccagga gcgcttagaa agtctcgccg gaggcgcttt gcagcttttt
     3901 gaccagcacc accagttgcg gccgtacctg ggtcatcaaa aactggggtg aaagctggaa
     3961 ccccggacca ccgcaattga tcaccatcac agcgcgcccg cccggcggtc ggaaagccgc
     4021 ggcgatggca ttgacatctt tttgccactc gccaaacgag gcgcagcagc cgtgctcgcg
     4081 gtaatgcgcc agggccagct ccagcgcgtc c
//
LOCUS       DAIDFG010000281         4099 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_281_length_4099_cov_19.711177, whole genome shotgun sequence.
ACCESSION   DAIDFG010000281 DAIDFG010000000
VERSION     DAIDFG010000281.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_281_length_4099_cov_19.711177"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          703 a         1390 c         1305 g          701 t
ORIGIN      
        1 agccccacca gctcggcccg gccttcgtaa tacagccagg cggccagggc cgcggcaaag
       61 ccgtccggac agccgcggcc gtggtacagg atcaggggct gcgggtcgtt tctgtcgggc
      121 gcaaccagca gttgcagggg gagtatggtt ttcatggccc tccgattgtc gcccggcggc
      181 ggcatggcct ttccccggga tcagtcccgg ggcgccgccc cggtcggcgg ccggcggctg
      241 gcggcctctt cggcgctttc catgtgctcg ctcggccggt cggtgtgcga cacccagagg
      301 ccgtcggcca cataaccggg gcctttcatg atcatcacga tgacgcaacc aaaagccacc
      361 gtgaccacca tggaccagtg aaacaggatg gcgcccagca cgatgtagcc caccatctgc
      421 tcccagcggg cctgggccgc accggcggcg ggatcgttga acaggtacac cagccccagc
      481 accaacaggg gcagcagcgt gcccaccgcg gcaatcagcg gcagcttgcg ccacagcgtc
      541 cattccaggc cgctggcggc gcgccgggag ttcgggagtt ttttcaacca gttcatcgcg
      601 ggcacggcac tccatgaaaa gcttgttgcg tggtgtccag tgttccacgt cggggaccgc
      661 ccggccatga catggatcag ggggcattgc agaatgtttt ttagttttag cccagctgtt
      721 accgccctgc agcagcacgc acctttgtcc atgtcgagcg tgcgggagac tcccctcccc
      781 ccgccgaggc gggaaccaat ccgcgccagc cggctccatc gtgcaagcgt ttgcgcgcat
      841 ggccgcaaag cccccaagga agcccatgct cagaaccctc aaagacctgt ttgacacttt
      901 cgcggcgccc gcccgggcgc aatcccccgg cgagcgcgaa catgcgctgc agctgggcac
      961 cgccgtgctg ttggtggagg tcatgcgtgc cgaccccacc atcaacgccg ccgaaagggc
     1021 cgcggtactg gacacgctgc gcggaaaatt ttcgctcgcc gacgacgagc tggcccggct
     1081 gctggaactg gccgaggaca cggccaggac cagccacgac taccaccgct tcacctccgc
     1141 catgaacgag cacttcacgc aggcgcaaaa gatccacatg gtggagacca tgtggcaggt
     1201 ggcgtattca gacgcccacc tcgacgcgca cgaaaaccac ctgatcagca agattgccgg
     1261 cctgctgcat gtcacgcatg gcgaatacat aggcgccaag atgcgcgcca aggaggccgc
     1321 cggccatgcc tgagcgggcg tctggcatct gaaactgctc tgctattgat agctgcttcc
     1381 gcccgttcca aaagggctgt gggcactttt tacatactcc cgccagcccg tctcagtgtg
     1441 atggtttgac ggccgcagcg gcgagaatcc tgaggagtct ggaagtgttc atgcaaggac
     1501 cttttatgcg tccggcgcgc acccatccag tcatcaaaag ctcagcccct ggccggcttc
     1561 ttcaatcgtc tggccatcac ggatgtgata gatgcgcttg aacgtgggga tgattttttc
     1621 gtcgtgggtc accacgatga tcgcggtctg ggcctgccgg gccatctggt tgaggatgcg
     1681 catcacggcg agcgcccgtt cgctgtccag cggggcggtg ggttcgtcgg ccaggatcac
     1741 aggcggacga ttcgccaacg cccgcgcaat cgatacgcgc tgctgctcac cgcccgacag
     1801 ttgcgagggc tcggccctgg cgcggtgggc cacatccagc gcggtcagca gttccagcgc
     1861 ccgggccctt gcctgggcgt tggatacacc agccagcatg ggcagcaggg ccacgttgtc
     1921 ggtgatgtcc agaaagggaa tcaggtacgg tgcctgaaac acaaagccga tgcggtcgcg
     1981 ccgcagggca cgcaggtcgg aggtggtcca gccgttgtcg taaatcacat cgttgcccag
     2041 cgtcatgcgc cccgacgtgg gttcaatgat ggcacccagg catttgagca gcgtgctttt
     2101 gcccgagccc gaggggccca ccagtcccac gacctcgccg gggcccacgg tcatgttgac
     2161 gttcctgagt gcctcgacgg cggtgtcgcc ctgcccatac accttgcgca ggccttcaat
     2221 gcggatgccg ccggcgcttg ccggagttgc cggggtcatc cgatcgcctc tgcaggatcg
     2281 accttgagtg ccgcgcggat ggccagggtg ctggccagcg cacagatcag cagcgtgccg
     2341 cccagaccca tcatggcgtc ctgcgtcagc agcagcacaa acttgggaaa caccggcgcc
     2401 cagagggtgg cgctcacctt gccgacgaca aaaccaatca accccaggcc cagcgcctgc
     2461 tgcaggatca tgctggcgat cgtcaggttg cgcgtgccga tgagcttgag caccgcgatc
     2521 tcgcggatct tgcccagtgt catggtgtag atgatgaagg ccacgatggc tgcgctgacc
     2581 accgccagaa tcaccaggaa catgccgatc tgtctggccg aagtggcaat cagcttggcg
     2641 accagaattt cctccatgcc gtcccgcgtg aacacctcca ggtgcttcca gcggcgaatc
     2701 ggctcggcca cctgcccggc ctcgaacccg ggtgccagtt gcaccagcac ggcattgacg
     2761 ttgtggctgc tggtctgcag cgcctgcact gcatcaagca ggcccggcac gccggggcgg
     2821 ttgaaggccg ggttggtggc ggttcgggtg cggtcgttga cgatggcgtc gttgtctttc
     2881 aggaactgcg cctcctgggc gtccttgagc gggatgaaca ccatcgggtc gccgcccgag
     2941 gacaccatgc gttgcgtcag tcccaccacg gtgtagtcgt ggcggcggat gcggatgcgc
     3001 tcgcccaggg caaagccggt tttcacatcg gccacggctt cgtaatggcc gcgtgtgagc
     3061 tggcggcccg ccaccaaatg gcccggctcg ccaggctggc cggattccat cccgaccacc
     3121 atggcgcgaa tgtcgtcgcc gtcgtggctt tgaacctgca tggtgaagta ggtcacgttg
     3181 gctgcctgcg ccacaccggg catgccgcgc acgctgcgca ccacatcgtc tttcaggctc
     3241 gacgattcgg catacggccc ctgcgtgtct ttttgcacca cccacaggtc ggcccggctg
     3301 ttggtcagca gggcctgtgc gtcatcgacc atgccgcggt agacaccggc catggtcagc
     3361 gtcacaccga tcagcaggcc caggcccagg cccgtgagca caaacttgcc ccagctgtgc
     3421 gcaatgtcgc gcccggccag actgatcacg gtgaggtccc tttgcgtccg tccttgacca
     3481 gcgtgtcgac cacctgcacg cgggcgccgg gggtgagggc cttctggctg tacaccacca
     3541 cctcgtcacc ggctgtgagg ccggccatca cctgcacctg gccatccaga ctgcgaacgc
     3601 ccagccgcac cggcgcgaac accggcttgc catgttcgat gcgccagaca ccggtttcac
     3661 cctggcgatg ctggatgctg gcaccgggca ccagcagtga ggaagctgtt tcgggcagct
     3721 gcagcgtgac ttcggccagc tcacccacgg aggcacccat cgcgctggca gtcggcgcct
     3781 gcgcaaaggc cacctgggcg atacgctcct cggtcacgct gtcggccagc agctccacgc
     3841 gcgcaacctg gcccggcatg ggcgcacccg gatgcgaacg cagcacgatg ctggcggtca
     3901 agcccggcgc gaggccggcc gaacgcccct ggtccacccg cattttcacc cacaggctgg
     3961 ccgggtcgat caggcgcagc acaggctggc cggccaccac ggtggtgccg gcttcggcgt
     4021 cgcggctggt aaccactcca tcggccgggg ccagcagacg gacgttgccg cgttgctgtt
     4081 gcaggccagc gcgctctgc
//
LOCUS       DAIDFG010000282         4088 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_282_length_4088_cov_20.070419, whole genome shotgun sequence.
ACCESSION   DAIDFG010000282 DAIDFG010000000
VERSION     DAIDFG010000282.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_282_length_4088_cov_20.070419"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          649 a         1310 c         1361 g          768 t
ORIGIN      
        1 gcttttcttc cagccgccaa agccgtgcca ggccatgggc acgggaatcg gcacgttgat
       61 gccgaccatg ccgacctgga tgcggcgcga gaattcgcgc gccacattgc cgtcgcgcgt
      121 gaagcagctc acgccgttgc cgaactcgtg ggcgttgatc aggttcaccg cttcggccag
      181 gtccttgaca cgcacgcagc ccagcaccgg accgaaaatt tcttccttgt agatcttcat
      241 ctccggggtc acatggtcga acagcgtgcc gcccatccag aagcccttgt cgcaaccggc
      301 gcccgcgctt tgaccggtga agccgcggcc gtcgaccagc agtttggcgc cttcctggac
      361 gccggtgtcg atgtagccgg tgatgcgctg gtgggctgcg gccgtgacga tggggcccat
      421 ctcggcatcg agctcgaccc cgttcttgac gaccagtgtt ttggcacgtg cagccagcat
      481 gggaacgagt ttgtcggcga cgttgccgac cagcaccgcg accgagatcg ccatgcagcg
      541 ctcaccggcc gaaccgtaac ccgcgccgat gagtgcatcg accgcctgct ccaggtcggc
      601 atcgggcatg accaccatgt ggttcttcgc gccgcccagc gcctgcaccc gtttgccgtg
      661 gtgcgcgccg gtttcgtaga tgtagttggc aatcggcgtg gagccgacaa aagacacggc
      721 cttgacgtcc gggtgcacca gcagggcatc gaccgcttcc ttgtcgccct gcaccacgtt
      781 gaacacgccg tcgggcaggc cggcctgttt gagcagctcg gccatgtaca gactggcgct
      841 cgggtcgatc ggactgggct tgaggacaaa gcagttgccc gcggcaatgg ccaccgggaa
      901 catccacatc ggcaccatga cggggaagtt gaaaggggtg atgccggcca ccacgcccag
      961 cggctggcgc agcgtccagt tgtcgatgcc ggtgctcacc tggtcggtga agtcgccctt
     1021 gagcagctgc ggaatgccgc aggcgaactc gacgatgtcg atgccgcgcg agacctcgcc
     1081 ctgggcgtcg gtgaagacct tgccgtgttc ggccgtgatc atgtgcgcca gtttgtcctt
     1141 gttctggttc agcagttcga ggaacttgaa catcacgcgt gcccggcgta tcggcggcgt
     1201 gtcggcccag gccggaaagg cggcctgcgc cgcgctgacg gcagcgtcca cctcggtggc
     1261 attggccagc gccacgtggc ctgtcacggc gccggtggcg gggttgaaga cgtcctgtgc
     1321 gcgcccggag ttgccgggca cggttcgtcc gttgatggcg tggccaatgg cctggatgct
     1381 gctgttcata tttgggcggc tcatatcatt catggggcgg tctcctgcaa ttaacctgtg
     1441 atcaatcaaa taggttagcg gaccaggccg acttttgcta gtttgaattt ctcagaagtg
     1501 ttataaatat tactaataat ggacatccag acactccgga tttttgtggc ggtggcccgc
     1561 gagggcagcc tgtcgcgcgc tgccgacaag ctggccctga cccagccggc cgtcagtctg
     1621 cagattaaag gcctgcaagc tgccacgggg ctgacactgt tcaagcgcag cgcgcagggc
     1681 atgaacctga cgcgcgacgg cgcggccctg ttgccgctgg ccgaaaaagt gctggccgcg
     1741 cagggcgact tcaaaaacgc cgccgcccgc ctgcacagcc aggtgcgcgg ccgcctgcgc
     1801 ataggcacca tcctcgaccc cgagttcacc cggctcgggg cctttctgaa ggtgctggtg
     1861 gaaaccgcgc cccaggtgga aacggaactg cgccaagcca tgagcggcga ggtgctggcg
     1921 caaatcgacc ggggcgatct cgatgtgggc ttttacctcg gcacgccggc ccagccgctg
     1981 ggggagaccc atgattttgt taagaatcag actgcagccc tttccctaca agcgtcggca
     2041 gctatacatt caggagctga tcaggcgccc ggccgggtgg cacgttttgc cgtccgggag
     2101 ctgacgcact tcacctaccg ggtgctggcg ccggccggct gggggccgca ggtgcgcggc
     2161 aaggactgga aagccctggc ggccttgccc tggattggca cgccgcctgc ctcggcgcac
     2221 caccggctgc tggccagtgt gttcggcccg ctgggtgtcg agccgcaccg ggtggcgctg
     2281 gtggaccagg aggcgtccat gctggatctg gtgaagtccg gcgtgggcct gagcctggtg
     2341 cgcgactcga tcgcgatgcg cgagtcgcac gccgcgggcc tggtgattgc cgaccaggtc
     2401 agcctgcaaa gcgtgctgag ttttgtcagc ctgagcgcgc agcgcgaggc gccggtggtg
     2461 gccagtgcct ggcacgcgct tgaggcggtc tggggcacgg actgactcct tcgccaggtg
     2521 cccccgggcc ccgcccgtcc atgcaagccg cgtccaccgc gctgtaggac agggcggagt
     2581 atggattggg gacaaatgct gtagcgcctg cgccggccgc tgctttaata tgcaatgaat
     2641 gggcggctgt cacgcgtgtg gaccggagcg ccgccctgtt ccgcccatcg atcccaagct
     2701 gtttgcacct atcgaccaca tgccatctcc ggaggacgac gccgcgcgcg aatctgctcc
     2761 gcacatcgac ccgcaagaca cgcccgaagg tgcgtgttac cgggtgctgg gcccgtggac
     2821 ggccgccgag ctgacggccc agccggcgtg gcaggcgctg acgctccagt tgaacgcgct
     2881 ggatgctgcg gcagccggca ccgccctgtg ggacttgcgc cagatcgacc agctcgacca
     2941 tctgggcgcg caggtgttgt gggaccactg gggccggaag tggcccgaga agctggaggt
     3001 cctgccgccg caacgcgcgg tgctggaccg cgtggcccag ttcagtaccg accggcccga
     3061 accgccggcg ccgggtttcc gggaaaattt cctgtcgctg ggcgaaggtg ttctgaaggt
     3121 ggtggatcac ggcgcggacc tgctggtcct ggtgggccgg ctgctgctga acctgatcaa
     3181 gctggtcaaa aatccgcgcg aaggcccctg gcgcgacctc tccggccacc tgtaccacat
     3241 cggtgcgacg gcactgccga tcaccgccct ggtcggcttc ctgatcggtg tggtgctcgc
     3301 ctacctgatc tcgcagcagt tgcgccagtt cggcgccgac gccttcatcg tcaacatcct
     3361 gggcatctca ttgatccgcg agctcgggcc ggtgctcgcc gccatcctga tcgcagggcg
     3421 ttccggctcg gccatcacgg ctcagatcgg cgtgatgcgg gtgaccgaag agctcgatgc
     3481 catgcgcgtg atgggcattg cccacagtta ccggctggtc atgccgcgcg ccatggcgct
     3541 ggccatcgtg atgccgctga tcagcgtctg gaccaccatc gcggccctgc tcggcggcat
     3601 gctcgctgcc gacctggtca tgggcgtgac gccgtccttc ttcctcgatg ccctgcccga
     3661 ggcggtggac gccgccaacc tgacgctggc cacggccaag tcggtcgtgt ttggtgtgct
     3721 gattgcgctg atcggttgcc atttcggcct gcgtgtcaaa cccaacaccg aaagcctggg
     3781 tcagggcacc acggcctccg tggtcacctc gatcacggtc gtgattctgg tcgatgcact
     3841 ttttgcagtt ttgttcaaga gtatcggtgt atgacttggc ccccacgttc gctcactgcg
     3901 tgtagctctc tgccccccga gcgggcgctg cgcctgcggc ccggcgaagc cggttccgcg
     3961 gcccctgctg gaaggtgtgg ggtaactttt ggtggaacaa cgctggggtg tgcgcaatga
     4021 gtgatgccat tgtggatatc cgcgatctgt ggacggtgtt caaaaacggc aagaaagagg
     4081 ccgtcatt
//
LOCUS       DAIDFG010000283         4087 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_283_length_4087_cov_16.244544, whole genome shotgun sequence.
ACCESSION   DAIDFG010000283 DAIDFG010000000
VERSION     DAIDFG010000283.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_283_length_4087_cov_16.244544"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          807 a         1342 c         1198 g          740 t
ORIGIN      
        1 aatcacgcgg ctgtcctccc atctggacga aatcgaccgg ctgctggccg ccggcggcga
       61 agtcggcaaa cgtctcgact tcctgatcca ggagctgcac cgcgaagcca acacgttggg
      121 ctccaaatcc gggtcgctgg aactcaccca tgtctcggtg gacatgaaag tgctgatcga
      181 gcagatccgc gagcaggtcc agaacattga ataaggccag ccccggccag aacaacaacc
      241 tacaccaccc gccgatatgg actacccagg aaatctcttt gttgtcgccg cgcccagtgg
      301 agctggcaaa tccagcctgg tcaaggccct gatggagctc gatgcgcggg tgcagccggc
      361 ggtgtcgcac accacccggc cgccccgcgg gcaggaaaaa catggccgcg aatacttctt
      421 catttcgccc gaggagtttg acggcatggt gctgcgggac tccttcctgg aatgggcgca
      481 tgtccacggc cagcgttacg gcacctcgcg gcaaacgatc gaggaacgcg ttgcaaccgg
      541 tgccgacgtc attcttgaaa tcgattttca gggcgcgatc aacatcaagc gtattttcaa
      601 caacgcggtg ctgattttca tcctgccgcc cagctgggaa gagttgcgct cgcggctgga
      661 gcggcgaggc gaggatacgc cggaagtgat cgaattgcgg cttaaaaacg cagccatcga
      721 gatggcccag gcccacgaat tcgatttcgt tataatcaac gagttatttg aacgagccgt
      781 tttcgacctg aaaaccattg ttcatgccca gcggctcaag tttgcagccc aaagacgtgc
      841 ccgcgccgag accttccagg ccctgcacat tgcctgacct gctgctgctt cattccccct
      901 attgcattta acccggagat catcatggcc cgcatcactg ttgaagactg cctggaaaaa
      961 atccccaacc gcttccagct cgttctggcc gccacttacc gcgcgcgcat gctcagccag
     1021 ggccatgccc ccaaaatcga gagcaagaac aagcctggcg tgaccgcctt gcgcgaaatc
     1081 gccgaaggca aaatcggcct cgaaatgctg aaaaaagtcc ccagctgagg cgtcctccct
     1141 ctcctccaga aaagcaccgc ctgcggtgct ttttttgttt ccctgacgtc gcgtaatgcc
     1201 cgctttttgc acgttttatc agcaccacga tacatttaag gcatgagcgc cctggatttc
     1261 cccatcgcaa acgtcacctc caccccggct gccgccaacg cagccgcggc gagttttgct
     1321 gcgctcacgg ccaagctgga ctacatgagc gccgaggacg tcaacaacgt ccggaccgcc
     1381 taccgctttg ccgatgaagc gcatctgggc cagatacgca acggcggcct gccctacatc
     1441 acccacccta tcgccgtggc gcagcagtgc gccgaatgga agttggacgc gcaggccctg
     1501 atggccgccc tgctgcatga tgcgatcgag gactgcggcg tcaccaaacc ggagctgatc
     1561 gagcgttttg gcgcgccggt cgcggaactg gtggacggcc tgaccaagct ggaaaagctg
     1621 gaattcaaca cccgcgagga aaaccaggcc gagtcgtttc gcaagatgct gctggccatg
     1681 gcacgcgatg tgcgcgtcat cctgatcaag ctggccgacc gcagccacaa catgcgcacg
     1741 ctcagcgacg tgccgcgcag caagtggaac cgcatctcgc gcgagaccct ggacatctat
     1801 gcgccgattg cgcaccgcct gggcctgaac gcgacctacc gcgagctgca ggacctggcc
     1861 ttccagcacc tgtacccctg gcgttacgcc acgctgacca aggccctggc ccgcgcccgt
     1921 ggccgccggc gcgatgtgat caagcgagtt cagtccgaag tggaagcagc gtttgcgagc
     1981 gccggcatcc aggtgcatat ctacgggcgc gaaaaggccc tctattcgat ctatcgcaag
     2041 atggatgaaa agcacctgtc atttgcgcag gtgacggaca tctacggctt tcgcatcatt
     2101 gtcgccagcg tcacggcctg ctacaccgcc atgggcgtgc tgcaccagct ctacaagccg
     2161 ctgccaggca agttcaagga ctacatcgcg attcccaagg tcaatggcta ccagtcgctg
     2221 cacaccacgc tggttgggcc cttcggcacc aacatcgaat tccagatgcg caccgaggcc
     2281 atgcacatcg tggccgaatc cggcgttgcc gcacattggc tctacaaggc caccgaccct
     2341 gacagcgatg cctcgcagcg gctgggcacc cagtggttgc agtccctgct cgacatccag
     2401 caggaaacgc gggacgccgc cgagttctgg gaccatgtca agatcgacct gtttcccgac
     2461 gccgtgtatg tgttcacgcc gaaaagccag atcatggcca tgccgcgcgg tgcaacgatt
     2521 gtcgattttg cctacgccat ccacagcgat gtggggcacc gcgccgtggc cgccaaggtc
     2581 aatggcgagc aggttccgct gcgctcggag ctgcacaacg gcgacgtgat cgagatcatc
     2641 accgcgtcgg tctccagccc caaccccgcc tggctgggtt ttgtgcgcac cggcaaagcc
     2701 cgatcgaaga ttcgccatca cctcaaaacc atggccctga gcgagtcgca ggacctgggc
     2761 gagaaaatgc tggcgcaagc cctgcgggcc gagggcatag accgcgagag gctgtccgag
     2821 gacgacgacg cccaccacgc cacctgggaa aaaatcctgc gtttcagcgg caaccgcaca
     2881 cgcgccgagt tgctcaccga tatcggcctg ggaaaacgga tcgccagcat gatcgccaag
     2941 cgcatcgtca ccctgctggc ggaaacaggc gaaaaaccgg accccttgct gatgagccgc
     3001 gagcgctaca ccgcgcacga gtcgatttca cagggcgcgc tggtgctgga tggcagcgag
     3061 ggcgcctcgg tgaagtttgc cgcgtgttgc cggcccatcc cgggcgacgc catcgtgggt
     3121 tatctggggc gcggtgaagg cctggtggtc cacacggacg actgcgccgt cgccaaaaag
     3181 ctgcagcacc gcgacagcga gcgcttcatt gcggttgact ggtcggacga acccacccgt
     3241 gcctttgaaa ccggtctgct ggtcacggtc accaatggca aaggcgtgct cgcccgcgtg
     3301 gcttcggcgc tggccgccgc cgaggccgac atcacgcatg tggacatggg acaggaacct
     3361 gcgcaggatg cgaccgacct gcgctttgat gtggccgtgc gtgatcgcat ccacctcgcg
     3421 tctgtcatgc gcagcgtcaa gcgtaccccg tcggtgttgc gggtccagcg cgccaaacca
     3481 ggcgtctgaa cgccccccgg ggctgtttac ttcatgtcct gcgctcgccc gccggcagcg
     3541 tccggcgccg gataactgac ctgcatcacc tcgatgtcct tgagtccgcc cggggttgcc
     3601 agcttcacct cgtcgccgat ccgcgccttg agcagcgcac gggcaatcgg cgagatccag
     3661 ctgacctggt tgcgcgcact gtccgcctcg tcaataccca ggatggtcac cgtctgtccg
     3721 gacccgctgc tgtcgatgta ggtgacagtg gcgccaaaaa acacctggtc gctgccatgg
     3781 tgcacggcag ggtcggaaat ctcggcaatt tcaagccgct tggtcaaaaa ccggatgcgc
     3841 cggtcgatct cgcgcagccg cttcttgccg tagtggtagt cgccgttttc ggagcggtcg
     3901 ccgttgctgg cggcccagtg aaccacctcc accaccttgg ggcgttcgtt gtcgatcagc
     3961 tccagcagct cggccttgag ccgggcgtaa ccctcgggcg tgatgtagtt tttgccgccc
     4021 gcgggcaaag gcggcaacga cagctcgtca tcgtcgtccg cgtcggtttc tttggtgaag
     4081 gctttgc
//
LOCUS       DAIDFG010000284         4048 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_284_length_4048_cov_20.110694, whole genome shotgun sequence.
ACCESSION   DAIDFG010000284 DAIDFG010000000
VERSION     DAIDFG010000284.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_284_length_4048_cov_20.110694"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          716 a         1372 c         1266 g          694 t
ORIGIN      
        1 tggcgaagca aagaaaagta agtcgcccgc cggggcgagt cccggcttgc tggcacacca
       61 cagcagctgg cgtgaaaagg tgactgcgcc ttcagcccta cggactagcc gcgccccaca
      121 aatggcatct tcgtcgccat gatggtcatg aactgcacat tggtcgccag cggcaacgcg
      181 gccatgtgta ccaccgcgtc ggccacatgg ttcacgtcca tcaagggttc gatcgcaatt
      241 tcgccattgg cctgcggcac acccttggcc atgcgcgcgg ccatctcggt ctgggcgttg
      301 ccgatgtcga tctgcccgca ggcgatgtcg tattggcggc agtcgagcga gatcgacttg
      361 gtcaggccgg tgattgcgtg tttggtggcg gtatagggcg ccgagtgggg acgcggtgcg
      421 tgcgccgaga tcgagccgtt gttgatgatg cggccgccct gcggcgactg cgccttcatc
      481 aggcggatgg cctgctgcgc gcacaggaag gagccggtca ggttgatgtt gacgacgttt
      541 tgccattgct cgaacgtcaa ttcttccatg ggcacggcgg gcgcaccgac gccggcgttg
      601 ttgaacagca ggtccaggcg gccaaaggcg gcgtgaacct tgtcgaaggc cgcacgcacc
      661 gcgtcggggt tgctgacatc gcagggcacg gcgagtgccc tgtcgccagc ggcggacgcc
      721 tgggctacct cgttcaaagg ctccacgcgc cggcccacga gcgccacgcg gtaaccctca
      781 cgcagcagcg ccagcgccac cgtcttgcca atgccggtgc ctgcgccggt aacgagtgca
      841 accttgccgt ttgatgtcat gggtgagcct ttgtctctgg atgttgtgga ggaaaggacg
      901 gcctgccagc acgtggcccc gccctgcgca gattgtgccc cagcaacacc ggtggcgctg
      961 gcgcccgggt gccgcagcag gcggcgtccc gggcaactgc tttaggtttc aactattttg
     1021 aaaagctgtt ggaaattacc gacaccacgc catttgcagc agtgctacct ttgcgcgatg
     1081 acgtcccgcg ccacccctgc acccactgaa cacgctgccg cccggccgcg gaaaatctgg
     1141 gacatctcgc ccgccgtgga cgagcaggcc gccgtgtttc cgggcgacgc agcgtattcg
     1201 cagaagctgc actttgcgct ctcgcccggc tgcccggtca atgtcaacac cctcaccttc
     1261 tcgccgcaca ccggcgccca tgccgatgcc cccatgcact atgccaacgg cgcgccggcc
     1321 attggcgccg tggatctgca gccctacctc gggccctgcc gcgtgatcca ctgcctggac
     1381 tgcgggccgc tggtggagcc gcagcacatc gcccacgcgc tggaaaacct gcccgcgcgc
     1441 gtgttgctgc gcacggccgc ggccgccagc cagtcctggg catcgtttac ggcgattgcg
     1501 cccgccacgc ttgcgctgtt agctacaaaa aacatagcac tcatcggcat cgacacgccc
     1561 agcgtggacc ccgccaccag ccaggacctg cccagccacc acctgctgct caagcacgat
     1621 ctgcgcgtgc tggaaaacct ggtgctcgac gaggtgccgg aaggcgacta cgagctgatt
     1681 gccctgcctc tcaaactgac aaaagccgac gcctcgcccg ttcgcgccgt gctgcgcgaa
     1741 ctgcccggga acggcgctgg aaacccatga ccacacttaa tgactgccgc gcgctcgacg
     1801 ctcgcaacgc gctgcgcccg ctgcgcgacc actttgtgct gcccgagggc gtgatttacc
     1861 tcgacggcaa ctccctgggc gtgttgccca gaagcacggc ggcacgtgtg gccgaagtca
     1921 tcacgcgcga atggggccag gacctgatcc gcgcctggaa cagcgccggc tggtttaccc
     1981 tgccgcagcg cctgggcgac aggattgcgt cgctgattgg cgccgggccg ggcgaggtgg
     2041 tcgccaccga cagcacctcg gtcaatctgt acaaggtcct cagcgcggcc ctgaacatgg
     2101 ccgccgaaga tgcaccggca aaaaaagtga tcgtcagtga acgcagcaac ttcccgaccg
     2161 atctctacat tgccgagggc ctgtgccgcg agcgcggcta ccgcttgcag ctggtcgagc
     2221 ctgaggaaat cactgccgca ttgacgcaag acgccgccgt gctgatgctg acccatgtga
     2281 actaccgcac cggcgccatg cacgacatgg cggcgctcac cagcgccgcc catgcggcgg
     2341 gtgttctggt ggtgtgggac ctggcgcaca gcgcgggcgc cgtgccggtg gacctgcagg
     2401 gcgccaacgc cgactttgcc atcggctgcg gctacaaata cctcaatggc ggccccggcg
     2461 caccggcttt cgtctgggtg catccccggc acgccgaccg tttccgccaa ccgctgtccg
     2521 gctggtgggg ccatgccgcg ccgttcgagt tcacgcccga ctaccagccc gcccccggga
     2581 tcacgcgtta cctgtgcggc acccagccca tcctgagcct ggccgcgctc gagtgcgggc
     2641 tggaggtgtt cggcgccgcg gacgccctcg gtggcatggg tgcgctgcgg accaagtcgc
     2701 tggcactgac cgacctgttc atcacgctgg tggaagaacg ttgcgccggc catggactgg
     2761 gactggcgac accccgcgag catgcggagc gcggctcaca ggtctgcctc tcgcgcagcg
     2821 aaggcgccta cgccatcgtg caagcgctga ttgcgcgcgg cgtgatcggc gacttccggg
     2881 ccggctccgg cgacggccgg catccggata tcctgcgctt cggcttcacg ccgctgtaca
     2941 tcgggtttga ggatgtctgg catgcggtcg agcatttgaa gcaggtgctg gaaaccggcg
     3001 agtggcaacg ccccgaattc aaccagaaac acgcggtgac ctgaccatga ccacccccga
     3061 aaacatcgtc accgacgaaa aagccaagct cgatttcagc cgcgacatga gttacggcga
     3121 ttacctccag ctcgatgcga ttcttggcgc gcagaagccg ctttcccccg cgcacgacga
     3181 gctgctgttc atcgtccagc accagaccag cgaactctgg atgaagctga tgctgcacga
     3241 gctgggcgca gcgattgcga acgtggctaa ggacgaactc ggcgacgcct tcaagaaact
     3301 cgcacgcgtc agccgcatca tggagcaact ggtacacgcc tgggacgtgc tggcgaccat
     3361 gaccccgccc gagtacagcg cgatacgccc ctacctcgcc aactccagcg gcttccagag
     3421 cgcgcaatac cgctgcatcg agtttgccct cggcaacaag aacgccgcca tgctcaagcc
     3481 gcacgcacat cgccccgatt tgctggcgca ggtgcaggcc gcctttgaag cgccgtcgct
     3541 ctatgacgaa gccctgcgcc tgctggcacg ccgcggcctg cccgtgccga tcagccacac
     3601 gcagcgcgac tggacccggg cttacgtgga aagcgacgcg gtcgaacaag cctggctggt
     3661 ggtctaccgc gaccccaaac agtactggga cctgtaccag ctcggcgaag aactcaccga
     3721 cctcgaagac gcgttcaggc tctggcgctt tcgccatgtg acgacggtgg aacgtgtgat
     3781 cggcttcaag cgcgggacgg gtggcaccgg tggcgtgagt tatctgcgca agatgctgga
     3841 tgtggtgttg tttccggaga tctggaaact gcggaccaat ttgtaagaca aatagggtcc
     3901 tagccctttt cctgcggtcg ccagtagcta tttaattgat agcgatatcg gcatcacgct
     3961 gctttcgcgg cgtgcgcagg cacagtaggg tctaactccc cactcccacc cctaacccct
     4021 ccctcgcccc gctgggcgat ggaggggg
//
LOCUS       DAIDFG010000285         4045 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_285_length_4045_cov_17.540852, whole genome shotgun sequence.
ACCESSION   DAIDFG010000285 DAIDFG010000000
VERSION     DAIDFG010000285.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_285_length_4045_cov_17.540852"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          659 a         1320 c         1216 g          850 t
ORIGIN      
        1 gacaggtcgc ggcaaggcgc gccttcgata cccttccggg tccgtcatgc cggccaggtg
       61 ttggcacata agccgccagc catcggagcc gcgttttcct caggttcgcg cgcccttcgc
      121 ggcgcgcggc cagcacgcca agcctcgact tcgcaccctc gcgggcatcc gcgaggggcc
      181 gcgacggtca gcgcggcagg tcgctgaagc ccatcaggaa ctcgtcgacc gagcgcgcca
      241 cctggcggcc ctcgcgaatc gcccagacca ccaggctctg gccccgacgc atatcgcccg
      301 ccgcaaagac cttgggcaca ttggtggcat aaccaccggt gaagtcggta gtcgccttgg
      361 cgttgccgcg tgcgtctttc tcgaccccaa aagcgtccag caccggtgcc accggcgcta
      421 caaaacccat ggccagaagc accaggtcgg ccttcagcaa ctgctcggag cccggtacct
      481 cctgcatctt gccgtctttc cactcaacgc gcaccgtttt cagaccggtg accttgccct
      541 tgtcgccgat gaattccttg gtggaaatgg cgaattcacg ctcgcagcct tcttcatggc
      601 tggaactggt acgcagcttg atgggccagt agggccaggt catgggtcgg ttttcttctt
      661 caggaggctg cggcagcagt tcgaactgcg tgacgctggc tgcgccatga cgattgctgg
      721 taccgacaca atccgagccg gtgtcgccgc cgccgatcac gatcacgtgt ttgccatcgg
      781 cgcgcaactg gtccttgacc ttgtcgccgg cgttgacctt gttctgctgc ggcaggaatt
      841 ccatcgcgaa atggatgcct gcgagttcac ggcctggcac gggcagatca cgcgactgct
      901 cggctccgcc ggacagcacg atcgcgtcaa aatccttttg cagctgttcg ggcgtgatgg
      961 tttccttggc ccaattggtg actttcgagt cctttcccag cgcgtccttc gcccgtccga
     1021 ccatgacgcc agtacgaaag gtcacgcctt cggcctgcat ttgcgccaca cggcgatcaa
     1081 tatgggtttt ttccatcttg aagtcgggaa tgccgtaacg cagcaggccg ccgatgcggt
     1141 cgtttttctc gaacagggtc acgtcatggc cgacccgcgc cagctgctgc gccgcagcca
     1201 tacccgcagg acccgaaccc accacggcca cctttttccc ggtcctgtgt gcaggtggtt
     1261 gtggctggac ccagccctcg gcccaggcac ggtcgatgat ggcgtgctct atggacttga
     1321 tgcccaccgc gtcgtcgttc acgttgagcg tgcaggccgc ctcgcagggt gcggggcaga
     1381 tccggcccgt gaactccgga aagttgttgg tgctgtgcag ggtgtcaatc gcacttttcc
     1441 agttgccccg gaacaccatg tcgttgaaat caggaatgat gttgttgacg gggcagccgc
     1501 tgttgcaaaa cggcgtgccg cagtccatgc aacgcgcggc ctgcttgttg gcctgcgcat
     1561 cgtcgagccc gatcacaaac tccttgtagt ttttcagccg ctcgggcaca ggcttgtagc
     1621 cttcctcgat gcgctcgtat tccatgaaac cggtgacttt tcccatgagg ctactccaga
     1681 tacttcttgt tcaggctgag gctttggcgg ctttttcacc gaccggcttg gacgccgctt
     1741 tggcgggtgc ggctgtggcg agcttcctgg ccgccatttc gccgagtgcc cgcttgtatt
     1801 ccagcgggaa caccttgaca aacttgccgc gcgcttccgc ccagttgtcc agcaactcgc
     1861 gggcacgctt gctgccggtc cagcggttgt ggtcttcaag cagtttcttc agttgcgctt
     1921 catcggtctt gccgccgtgc cacaaggcat ggtcggaccc ggcctcctgc tgggcagcag
     1981 gctggacttt ttccagcgac accatggccg tgttgcagcg tttggcaaac tggccgtcct
     2041 cgtcgtacac gtaagccaca ccaccgctca ttccggccgc gaagttgcgc ccggttttcc
     2101 ctagcaccgc cacggtacca ccggtcatgt attcgcaacc atgatcgccc gttccctcga
     2161 cgaccgccgt ggcgcccgac aggcgcaccg caaaacgttc accggccacg ccgctgaaaa
     2221 aggcttcgcc ggtggtcgcg ccatagagca cggtgttgcc gacgatggtg ttcttgaccg
     2281 cgtcaccacg gaaatcgatg ctcgggcgta cgacaatgcg cccgcccgac aggcccttgc
     2341 cggtgtagtc attggcatcg ccgatcaggt acatggtgat gcctttggcc aggaaagcgc
     2401 cgaaagactg gccgcccgtg ccttcgagct ggatgcgcaa ggtgtcgtcc ggcaggccct
     2461 ccgggtggcg ccgcgtcagc tcgccggaca gcatggcgcc gacggtgcgg ttgacgttgc
     2521 gggccacctc gatgaactgg actttttcac ccttttcaag cgctgctttc gatttctcga
     2581 tcaggcgcac atccaacgcc ttttccagcg catggtcctg ctccatgaca tgtttgcgcg
     2641 gtacatcggc cggtacgttg ggctggtaga acaggcggga gaagtccagc ccgtcggcct
     2701 tccagtgctc gatgcccttc tgggtatcga gcaggtcggc acggccaatg agttcgtcga
     2761 acttgcgcac accgagctgc gccatcaatt ggcgagcctc ttccgccacg aagaagaagt
     2821 aattcacgac gtgttcgggc ttgccgctga atttctggcg cagcaccggg tcctgcgtgg
     2881 cgacgccgac cgggcaggta ttgagatggc atttgcgcat catgatgcag ccctcgacca
     2941 ccagcggcgc cgtggcgaag ccgaattcgt cagcaccgag caaggcacca atcacaacgt
     3001 cgcggccggt tttcatctgg ccgtcggcct gcacgcggat gcggccgcgc aggcggttga
     3061 gcaccagtgt ctgctgcgtc tccgccaggc cgatctccca aggagatcct gcatgcttga
     3121 tggacgacca tggcgaggcg ccggtgccgc cgtcatggcc agcgatcacc acgtggtcgg
     3181 acttggcttt ggccacgccc gcggcgatcg tgccaacgcc gacttcgctg accagcttga
     3241 cgctgatgct ggcgcgcggg ttgacgtttt tcaggtcatg aatcagttgt gccagatcct
     3301 cgatggagta gatgtcgtgg tgcggcggag gcgagatcag gcccacaccc ggcacggagt
     3361 aacgcagctg gccgatgtac tcgctgacct tgccgcccgg cagctgaccc ccctcacccg
     3421 gcttggcacc ctgcgccatc ttgatctgga tctggtcggc cgacacgagg tattcggccg
     3481 tgacgccgaa acgccccgag gcgacctgct tgatgcgcga acgcagcgag tctccgtcct
     3541 gcaagggcag gtcaacctca accacgctct cgccaatcac gctcttgagt gtctcgccct
     3601 ttttgatcgg gatacccttg agctcctgac ggtagcggtt cggatcttct ccgccctctc
     3661 cggtattgct cttgccgccg atgcgattca tggccacggc cagcgtggca tgcgcctcgg
     3721 tgctgatgga gcccaacgac atcgcgccgg tggcgaaacg tttcacaatt tccttggccg
     3781 gctcgacctc gtcgatcggg atggctttcg acggatcgat cctgaactcg aacaggccgc
     3841 gcagcgtcat gtggcggcgg ttctggtcgt tgatcagctg ggcgtattcc ttgtaggtgt
     3901 tccagttgtt cgaacgtgtt gaatgctgca gcttggcaat cgcatctggc gtccacatgt
     3961 ggtcttcacc gcgcacgcgc caggcgtatt cgccgccggc gtccagcatg ttggccagca
     4021 ccgggtcgtt gccgaaggcg gcctt
//
LOCUS       DAIDFG010000286         4029 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_286_length_4029_cov_12.408153, whole genome shotgun sequence.
ACCESSION   DAIDFG010000286 DAIDFG010000000
VERSION     DAIDFG010000286.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_286_length_4029_cov_12.408153"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT         1049 a         1322 c         1059 g          599 t
ORIGIN      
        1 cagccttgtc gcggggtgct atcgaaagaa ggtcggtcat agggggcaaa gccatgaaat
       61 tacgtatgtc agtacgtgtg atcaaaaaat agcggcggca gggccggata aacgcttgat
      121 acgatagctc agaaaggggg ccgagcggac tctttcgatg cgtacaggta tccgctaccg
      181 tgtgtagcaa tcaaatcgag gtgctctgaa aactcgccct tgtcaaacgg aaaccgttct
      241 gcatggatca acaccatccg gatcccgaag cgattcatta aattcagcac ttgagtggaa
      301 cgaatgcggc agctgtcgtc gcaaacaatg gcctgagaaa aagaagcagg cccgaaccaa
      361 tctcctacca actgaccgct gtaaaaaaag aaggcatttt caaaaccgaa gtttagaact
      421 cggtggccat gctgtgcctg cagcgcctgc gcccgctcca gcaaaatcac gtcactacgc
      481 cccgcgcgtt gcgcctgcac cgtcggctgc tgccactgct tgctgccagt gatccccaca
      541 tggaccaaca tcagcgccga tgcagcgacg gccagcaatg ccccagcgcc gcgcaccggt
      601 gggagtttcg cagcgcccca aaccagcaag tagcgcacag gcgccaaggc caacaacgcg
      661 cccagtggca ggacaggcat caggtagcgg gagacctggc tgacccagaa ccaaaaaagt
      721 accaataaaa cgactaccag ccaaatcagc agccacgggc ggcggcgccc ccatgcaaac
      781 accgggatta caagagctgc gcacaggaaa atggccttaa ctttcagcaa gccatcaagg
      841 aaattcgccc accgcttggg gactccgtgc actgcctgct ctgcaacctg ggcctgcaga
      901 tcctgcggtg tccacaggta ataaccaaac acactgccac cggcaggatg gattgggtcg
      961 cctgtaaccc agtagctgcg caaataccag aatacacaaa ctgccagaca ggcaaatcca
     1021 taccaggcca ggttgcgcac cgcttgacga gaaccccctg aaaaaaccaa cacccccatg
     1081 cccactgccg ctgccaccac cagcccttgc agcttgatgc cactggcaat gcccgcacac
     1141 agacccgcca gtaaaagcca tcggtgctct ttgcgaccat cggcctgtac ccagaccacc
     1201 gctgccagac ccgccgctgt ggcatacagc gccaacagca cgtccaggta ggcaaacccc
     1261 agaatgcttt tcaccaaggg cgtataaacg taaatcagcc acgccaacac gccccaggcc
     1321 gcatgcccca acagccagcg gcctgcgccc atcagcccca tcaaagtcac gacgaccggc
     1381 caggtggcca gccactgcgc cagaaaggca tccagcagca atccggcagc aaacagcaac
     1441 tgggcatagg ccgggaagta gggaaagcgc agccactcgt tcgccaccag cccatggtgc
     1501 tcaacgatgg tgcgcgccag cggaaggtgg taggcggtat cgtcccagtg accgggaaag
     1561 cgccaggctg ccatgcccat gaagaccaca ccgaccagca ggaccgccca ttcccacagc
     1621 gcaacacctg ctcccgccgc gccagccggc ccatcgagcg catcgccccg agccagggcc
     1681 tggggcggag gccgccgctt caaccacaag gctgccaggg ccacgggcac acaagccccc
     1741 aggatgccag gcacatggaa ggcgcccacc agcgccagcc ccaaaagcac cagcatggcg
     1801 ccaccgatgc ccaacacctg ctgaaagaag agcaccagca gggccggtaa gccgcgcgcg
     1861 ccgcctgcag cctgccaaat tgcgcgccaa agagtccagc ccatgagctg gaacagcaga
     1921 acggcgccaa ctccggcagc catcaccgac caggcaggga ctccgtatcc gctttgaact
     1981 gaaagagccg ccagcacccg gtcaggcatc cggctcccct gttgccggtg acgcttccag
     2041 caaatccggc tcaccgagcc ggcccagcaa acggctcaag ggttcagtgg gcgcactgct
     2101 gacgtcatag tgcagaaatg cgatcagaaa ctggatgccg accatgatgg gcagggcgct
     2161 cagcatgacc gttccactgg tggcgggctg gttgcgccag atactttcga tccagtgcca
     2221 gccgccgaac aataccccgg ccagcagcaa aaccaccccg aaaatactgt agagcgaccc
     2281 aacgttgaaa tcgcgcacaa ggtagttgta cacataacgc cgccataacc gcgacgcatg
     2341 cttgcgcatg aactccggga gcacctgctt gattcgcaac ccggactgct cgtccgcgta
     2401 gacgctatcc atgggcacat cgcgaaccac cgcccgaagg gtgttcaggc gaaaaagcat
     2461 atcgctctcg aagaaatagc gtttttccag cttgtccaga ggcagttcac gcaatgcggt
     2521 gatgtggata gcggtatagc cattggtggg atccatgatc cgccaataac cacaggtgag
     2581 tttggtcaag aatgaaaggg ccgcattgcc aaacaagcgc acgaaaggca tgccgcccac
     2641 cgattcaggc cggaaaaagc ggttgccttt ggtgtaatcg gccaggccca gcatgatggg
     2701 acgcacaaaa aacggcaaca accggggatc catctggcca tccccgtcga ttttcacgac
     2761 gacatcgacg tcatcctgga tggcctggcg ataggcagtc gcaatggcac cgcccacgcc
     2821 ttggttctca acgtgtaaca acacctgcac cctgggatcg gtgcagtgcg tccgaatgaa
     2881 ctccccactg ccgtcagggc atgcatcgtc caccgcataa atacgttcca ccgacccggg
     2941 tatggcatca atgacggcca tgacatgccg ggtcacccga tagcagggaa tggccaccgc
     3001 gacgcgcatg gcgttcaagg agtcacctgc gctgccaggg tcacgcccac cacgatgaag
     3061 cctccgccca ccagggtctg ccaatgcaag ggctcaccca gaaaatacca accgatcacc
     3121 gggacaaaca tgaaagccag gcccatgaag ggatacgcca gatgcaaggg cgcgctgcgc
     3181 aatatccaga cccaaagcac cgtggtgagt ccatacaagg caagcgccac cagcagccac
     3241 ccattcaaaa gccagtctcc caactgcgcc tgcacgggca atgccatggc ggcctttttg
     3301 aaaagcagtt gacccaggga aattccgacc acgcacaaca ccgtgagcaa catcaaagga
     3361 atcgacataa ataaaaataa gactccatgc ctgcagaacc gattttcagg aacccgtcat
     3421 tgcgacccag ccaaccacag gaaccccggc acttgccgcc tggcctttgt gccgcctggc
     3481 gtttgtatca gccgccagtc tgccgtttca gtccggacag gcgttcagcc cttgacccag
     3541 cccggtacct tgaaccgaac tatagccatg taagccacca cgtagctcac cacaaaaagc
     3601 acacagaaac agatcaacaa gatggtgttg ttccaaaaca gcactgccgg caccaccgtc
     3661 agcagcgtga agcaccatag atagggcgac gtgcggttat tgcgcatgag catgcggcgg
     3721 gactcgtcat catgaaacac cctgcgcacg atgcgccgat agatcaactg gtggaaatgt
     3781 aacgcatctg ccacccctgg cgacaccccg cgagctacct tgcggtagat ggaaaaaatc
     3841 gtctcccaca ccggataaat caacagcagc atgggaaacc acggcgagac atccgcattg
     3901 cgctgcacca gggaaatgct ggccatggca atcaccacgc cccagatata agccccgcca
     3961 tcgccggcaa acaacatgcc atgcgggtag ttccagacaa gaaacccccc ggtcgcagct
     4021 gccaaggtg
//
LOCUS       DAIDFG010000287         3981 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_287_length_3981_cov_17.168874, whole genome shotgun sequence.
ACCESSION   DAIDFG010000287 DAIDFG010000000
VERSION     DAIDFG010000287.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_287_length_3981_cov_17.168874"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          686 a         1231 c         1356 g          708 t
ORIGIN      
        1 tattcctcgg ggttcatggg cgggattgtc gcaggcttgc ggcgatggca agcgggccgc
       61 tgccgggatc acatccggat cgcgcgccac agcatcagcg ccacatccca ggccgtgagt
      121 ttgggacgct ggcgcaacgt ggcaaagtcg atcgcttcga tcttgtcgag gatgcgcagg
      181 ccgccttgca ccaccaggcg caattcccag cccgcgcgcc ccggcacttt cttcaccaaa
      241 ggcagacctt ttagcatgcg cgcccttgcc gaatggacgc aggccgctat caatttcgta
      301 gtaatcggat tcacattcag ggccagcagt tgcgcttcat ccacgccgtg cgcggcccag
      361 agctcctgcg gcaggtagat gcgtccgcgc ggaatgtcca cgctgaggtc ctgccagaag
      421 ttgatcagct gcaacgcagt gcagatgtgg tcgctctgca ccaacgacgc cgcatcgttg
      481 acaccgtaga ggtgcagcag caggcggccg accgggttgg ccgagcggcg gcagtagtcc
      541 agcagctcgg cctcggtggc ataacgctgc ttgacaacgt cctgctcgaa cgcgctgagc
      601 aggtcgtcca gcagcggcac cggcaactgg aactgcctga tcaccggcgc cagggcagca
      661 aagacggccg gccatcgcgc cgagaccggc cggccctcgg ccgcggcgaa caggtcggcg
      721 cggcaggccg ccaagtcggc caggcgctgg gccggcaggg cgtcgccttc gtcggcgatg
      781 tcgtcggccg tgcgggcaaa ccagtagatc gccgccaccg ccggacgcag gcgtggcgga
      841 cacagcagcg aggccacggg gaagttttcg tagtggctgg aaggcggctg caacggctgc
      901 gcaaggtcgg gatgggggcc ggaagtcatg ggctggattg tcgcttgaca agccttggga
      961 attaccctag attactaacc ggtcagtcat taacagagtt tttagtctcg cctgtttcct
     1021 gctttccagt ctttttcgcc accgtttttc ctcgatgcca tcatgaataa aaccgctttt
     1081 gccgtcctca gcagcgcctt gttgctggcc gcctgttcca agtcagctcc gcccgaggag
     1141 ccggtgcgtg ccgtcaaggt catgaccgtg ggaacggatt ccttcgccac cacttatgag
     1201 tttgccggcg aggtcaaggc ccaggtcgag tcgcggctgg ggttccgggt cggcggaaaa
     1261 atcatccggc gccaggccga tctgggtcaa cgtgtccagg ccgggcaggt gctggcccaa
     1321 ctggacccgc aggactacaa gctggccgcc gatgcggcgc gcgcccaggt cgcggcggcg
     1381 gcgacgcagc gcgacctcgc ctcggccgac tacaaacgct acaaggaact ccgggaccag
     1441 aatttcatca gcggcgccga actcgagcgc cgcgagacca cgctcaaggc ggcgcaggcc
     1501 cagctcgatc aggcgcagtc gcaactggcc gtgcagggca accaggccaa ttacgccgtg
     1561 ctggtggccg acgtgtcggg cgtggtgacg gccgtggaag ccgaagtcgg gcaggtcgtt
     1621 gctgctggca ccccggtgct gcgcatcgcc gccgacggcc cgcgcgacgt ggtgttttcc
     1681 gtgccggaag acaaggtggc ggccatcaag gtcggcatgc cggtcaaggt gcgggtttgg
     1741 gcgcagaacg ccgggctgga aggcaaggtg cgcgaagtgg gcgccagcgc cgacccgctc
     1801 acgcggacct acccggtgaa ggtggcgctg gccacccgcg agccgccggt gctgggcgcc
     1861 acggtgtacg tgcagccgca gggcctgagc gccgcgggtg tgcaggtcat caagctgccg
     1921 accaccgctc tgcgccagga aggccggtca acggcggtct gggtgctgga caaggcgagc
     1981 atgacggtga agtcgcaact gatcgaggtg gccacggccg acggcaacga ggccgtggtg
     2041 gcttcgggcc tgcagcccgg catgctggtg gtcagcgccg gcgtgcacgt gctctcgcct
     2101 ggtcagaagg tcacaattta tcagccaaaa gtggctgcag ccctttcccc gcctgcgcag
     2161 atagctatca atccggtagc atccgccgct gcggcaacgc tgcccctggc tgcgcctgct
     2221 gctgcgaagt gaggccggca tgacccaagt acaaccgaag aacagcttca acctgtccaa
     2281 atgggcgctg gaccatccgg cgctgacgcg ttacctgatg gtggtgctga tgctgctcgg
     2341 cttcgctgcc tacttccagc tcggccagga cgaggacccg ccgttcacct tccgtgccat
     2401 ggtggtgcgt acctactggc cgggcgccac ggcgcagcag gtggccgagc aggtgaccga
     2461 caagatcgag cgcacgctgc aggaagtgcc gtttgccgac aagatccgca gctactccaa
     2521 gccgggcgag tcgcagatca ttttccagat caaggactcg tcgaaagccg ccgatgtggc
     2581 cggtgtctgg tatgcggcgc gcaagaagat cggcgacatg cggggcacgc tgcccggcgg
     2641 cgtgcaggga ccgtttttca acgacgactt cggcgacgtg tatggcgtga tctacgcgct
     2701 gcaggccgat ggtttcagtt atgccgagct caagaccttt gccgacgacg tgcgccagca
     2761 gttgctgcga gtgcccgatg tggccaaggt cgagcagttc ggcgcgcagg acgaaaagct
     2821 ctacatcgag atctcccaaa aacgcctggc ccagctcggg ctggacttca accaggtcct
     2881 gaaccagctc ggccagcaaa acgcggtgga gtccgccggc gcgatccaga cgccgctgga
     2941 cgtggtgcag gtgcgggtgg ccggccagtt cgaagtggtc gagcagttgc gtgccatgcc
     3001 gatacgcgcc agttcgggca gccagctgcg gctgggggac attgccgagg tcaggcgtgg
     3061 ttacattgac ccgcctgccg tcaaggtgcg gcaccagggc cgggaggtca tcgcgctcgg
     3121 tgtctccatg ggcaagggcg gcgacatcat tgccctgggc aaggcgctga aggcgaccac
     3181 tgcgcgcatc ggcaccagcc tgccggccgg catcgagctg gtgcagatcc aggaccagcc
     3241 gaccgcggtg gcgacctcgg tcaacgagtt cgtgaaggtg ctgatcgagg cggtggtgat
     3301 cgtgcttgcg gtgagtttca tcgcactggg ttttcatcgc cgcccgggcc agcacccgct
     3361 gtggaagcgt tattacatcg acatgcggcc cggcctggtg gtgggcatca cgattccgct
     3421 ggtgctggcg gtgacctttc tggccatgag ctacttcggc atcggcctgc acaagatctc
     3481 gctgggctcg ctgatcattg cgctgggcct gctggtggac gacgcgatca ttgcggtcga
     3541 gatgatggtg cgcaagatgg aagagggtta cgacaaggtg cgcgccgcga cctttgcgta
     3601 tgaaatcacc gccatgccga tgctgaccgg cacgctgatc acggccgtcg gctttttgcc
     3661 gatcggcatg gccaaatcgg ccgtgggcga gtacacctac gcgatctttg cggtcactgt
     3721 catcgcgctg gtgctgagct gggtcgtgtc ggtgtatttt gtgccttatc tggggacctt
     3781 gctgctcaag gtgccgccgc atgtgcagga ggtggccgcc gggcaggaca gccctcacga
     3841 gatgttcgac agccccttct acaacacgtt tcgccgggcg gtgaactggt gtgtgcagta
     3901 ccgctggctc acgataggcg caacagtgct gttttttgcg ctcggcattg tcggcatggg
     3961 caaggtgcag cagcagttct t
//
LOCUS       DAIDFG010000288         3972 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_288_length_3972_cov_17.701557, whole genome shotgun sequence.
ACCESSION   DAIDFG010000288 DAIDFG010000000
VERSION     DAIDFG010000288.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_288_length_3972_cov_17.701557"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          663 a         1197 c         1271 g          841 t
ORIGIN      
        1 cggctgtgcg gggtgatgag ccagtttcgc cccggtggcc cgcagcccgg cagccatctg
       61 acctattccc cgtggtgtgt gcccacggtg atcaacggcg tcaaatgtgt ggtggtcaac
      121 agcgacctgc gcgacgccga gcccatggcc tgggactttg cgatcaattt cgccaaggac
      181 aatgacctgc agatcgccga ggcctgcctg attccggaca agccataaag ctactgcgcg
      241 ggcgcgatga agcgttgcgc ctgacgggaa tgaaaagggg cccgtctccc ttttcgtcgt
      301 catcctcatg tacccaaagt acattccggt gcctgcgctc cgtgcgcctt gcttcgcgcc
      361 cgctcgctac gctttctggc ttgtcctccg ggactgaagc gcaaaacaaa aaagccgtcg
      421 aaagacggct tttttgcttt gctggcagcg caccctgcag aacggcgaac tgaagatcag
      481 tccgggcggg ttgcctgatt gctcaggcgg tgggttttac gcggcgggag tggccagggc
      541 gagtgccttg accttggtgg acaggcggct cttgtcgcgt gctgccttgt ttttgtggaa
      601 gatgcccttg tcggcgatcg tgtccacaat gctctgcgcc ttggcgaaga gttcggtggc
      661 tttggtcttg tcgccggcgg cgacagcttt ttcgacgttt ttcacggcgg tgcggtattt
      721 cgaacgcagc gaggtgttgg cggcgttgag tttgctgtcc tggcggacgc gtttgcggcc
      781 ggaggccagg cgggggttct ttttcttggg tttggcggta gccatataag tgttccttta
      841 aatgaggaag ggtctgcgga tgatgcttag caaagcccac cattatataa cttccctcaa
      901 tgtgccgcct taaagcccgt ccgggcctgt gatccggggc gcacagggct cccggcatct
      961 gcctaaatca ggctgacatc cttcagtggc ttgagcacgc tggacggtgt catgccgaag
     1021 gtttggcgga acatgcggct gaagtgtgag gaatccacaa acccgccgtg caatgcggca
     1081 tgggtcaggt tgctgcttgt tgtcagcccg accatggcgg agcgtacctg ggaccatacg
     1141 cggtagctgc gaaaactcac gcccatttca gcgcggaaca agtggttaaa gcgtgaggcc
     1201 gacaagtgtg cggcggcccc ggcgcagtca cggccgagcg gtccctcgcg ctgcgtgcgc
     1261 aaggcgagca ggccgcgggc gacccgcgcg tcgatcggcg cgacctggtg gcaggtcaat
     1321 tgcagcagcg aatgcacatc gaaggtacgc aaatgcgcat ccatgcggct gcgcgaggtc
     1381 acccgcacag ctggctcgaa aatcaccccg ccttccacct gaaaatgggt gcgcagaccc
     1441 cgtgcttcca cagagtctgg ctcaaggtac atgagtgcga catgttcgcc gcaggggtcg
     1501 aatacatgtt ccacaccggt gttgatcagc gcgctgttgc aggtctgggg gtgctggttg
     1561 ggcaattgca ccgcgaagcg gcccgacagc cccatcagca gcaccggcga cgcatggcgg
     1621 tgccaacccg ttgccggcaa ctcgcccaca tacagagccc ggttggcgcc gagcgtcagg
     1681 gtattggttg cgcgcatagc ctaaacgtac aagtttttcg tcatgctttc tgtattctcc
     1741 cgaacgctgc tcgtcccccc tggccagcgg tatggatcca catccccatt acacggagac
     1801 ttccttgaaa cgtcttgttc ctgcatggtc tgcatttctg tttttattgc ttgccgcgac
     1861 ggtgttgatg tactacagct tgcctggcgc atttgtgcaa ccgttgttga gcctgaaccg
     1921 tgaattgtcc ggtctctcgg aaaaaacggt atcagtgggc gcgcatgaga tccattatct
     1981 tgttggcgga agcggcgagc cggtcgtctt gctgcatggc atttttgccg agaaggatca
     2041 ttgggttgac tttgcaaggc ccctgacccg ccgctatcag gtgattgcac ccgacctgcc
     2101 tggcttcggg gaaagcggca ggctgccgga tcagagctat gactacgcca gccaaacaga
     2161 gcgcctgaag gctctgttgg atgtacttgg tatccagcgg gtgcacctgg ctggaaattc
     2221 gatgggcggc accctggcgg cgctgtttgc catccgttat ccagcgcgcg tggcaagtgt
     2281 tgcctttatc ggtgcgccgc acggcatcaa gacgcccagg cccagcgaga tggacggatg
     2341 gatcgatgcc ggcaagacgc ccctgattgc gcacaacacg gttgagtttg agcagatgat
     2401 gtcgctgctg ttcaaccaac gccctttcct gccgtatccc atcctgcagg cgacgcaggc
     2461 cgatgcactg cgcaatgcac cgtcgaatgt ccgtctatgg acggagcaat tgaaagaccg
     2521 ctaccttctg gatgcgcaca ttggcgcctt gaaacagccc aacctgacgt tatggggcgg
     2581 cgatgatcgt ctgtttgatg tttcaggtgc ggacgtggtg catacgcgcc tgaaaaactc
     2641 ccgcatccag gtgctgcaag gggtgggaca cttgccaatg atggaggcgc cgaagtcaac
     2701 ggcacagctg tacgccggtt tcctggcgga cttttccacg cagcgttagg gaagcgctga
     2761 acccggagcg tgggccttaa actcccgttg tgtcactttt caaatccgcg tccaccgttt
     2821 ccctgttcac gctggtttcc cggatcaccg gtctggtgcg ggagctgctg gtggcctcga
     2881 cgtttggcgc cagcgccatg acggacgcct tcaacgtcgc tttccgcatt cccaacctgt
     2941 tccgtcgcct gtttgccgag ggcgcattca gccaggcgtt tgtgccggtg ctggccgcca
     3001 gcaaggccca gcacggcgag gcggccaccc aggtgctggt caaccgggtc gccaccctgc
     3061 tggcctgggc gctgctgctg acctgtgtga tcggggtggc ggcggccccg gtgctggtct
     3121 ggctgatggc cagcgggctc aagcaggagc cgcgcggttt cgaagcggcg gtattcatga
     3181 cgcgctggat gttcccctac atcggtttca tgtcgctggt ggccctgtcg gccggcattt
     3241 tgaacacctg gaagcgtttt gccgtgccgg ccgccacacc ggtgctgctg aacctctcca
     3301 tgattggcgc ggcctggttc ggtgcgccct ggctcaagac cctgggcatt gagccgattt
     3361 acgcgatggg cgccggcgtg atgttgggcg gagccctgca gctggcggtg caggtgccgg
     3421 ccctgctgcg gatcggcatg ttgccgcgca tgggcctggg ctggacggcc atccgcaacg
     3481 cctgggccga ccccgacacc aaaaacatcg cccgcctgat gatgccggcg ctactggggg
     3541 tcagcgtggc gcatgtctcc accatcatca acacccagat cgcctcgcac ctgacgcccg
     3601 gcagcgtcag ctggctgacc tacgcggacc gcctgatgga gtttcccacc gccatgctgg
     3661 gtgtggccat cggggtggtg ctgatgccgc agttggccgc cgccaaggcc gcgggcgacg
     3721 gtgacgccta ttccgccatg ctggactggg gcctgcgcat cgtcgtgctg atggccgtgc
     3781 cgagcgctgt cgcgctgctg acctttgccc agccgctggt ggccacgctg taccactatg
     3841 gcgccttcac cgaccgcgat gtgcagcaga ccacgacggc gctgatgggc tacggtgccg
     3901 gcctggtggg cctggtggcc atcaaggtgc tggcgcccgg tttttacgcg agccaggaca
     3961 tccgcacgcc cg
//
LOCUS       DAIDFG010000289         3961 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_289_length_3961_cov_15.406554, whole genome shotgun sequence.
ACCESSION   DAIDFG010000289 DAIDFG010000000
VERSION     DAIDFG010000289.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_289_length_3961_cov_15.406554"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          877 a         1186 c         1203 g          695 t
ORIGIN      
        1 gccggccacc caccaggtcg acgacgtgtt gcaggaaatc cgcatccgtg tcgacaagaa
       61 tgagcgggtg ctgatcacca ccctgaccaa acgcatggcc gagcagctga ccgactacct
      121 gaccgacaac ggcgtcaagg tgcgttacct gcacagcgac gtggacaccg tcgaacgcgt
      181 ggaaatcctg cgcgatttgc gcctgggcac cttcgacgtg ctggttggca tcaatttgct
      241 gcgcgaaggc ctggacattc ccgaggtcag cctggtcgcc atcctggacg ccgacaagga
      301 agggttcctg cgtgccgagc gcagcctgat ccagaccata ggccgggcag cgcgcaacct
      361 caacggccag gccatcctgt atgcggaccg catcaccgac tccatgagaa aggcgatgga
      421 tgagaccgaa cgccgccgga acaaacagat tgctttcaac ctcgaacacg ggatcacgcc
      481 gcgcagcatc gtcaaacgca tcaaggacct gatcgacggc gtctacagcg agaaatccgg
      541 caaggaagcg gaaaagctcg aactgcagaa ggcccttgtg gaggacatga gcgagaaaga
      601 cattgctcgc gaaatcaagc ggctggaaaa acagatgatc gaacacgcca aaaacctgga
      661 gttcgaaagg gcggccagcg tccgggacca gttgcatgtg ctcaaggcgc aggcgtttgg
      721 cgcgccgggt gtggacaatg tgccgccggt ggcgggttga gggcgaagcg cggaagcagg
      781 actttcgtca cgaatttcgc tctgtttgtc ttggacaaat aggctcgagc ggtacaatga
      841 cgagtgaatc ggtcgggctt ctgtgctata cttgacgaaa atagtcagga acaaacagaa
      901 caacaaaagc cctgcagccg atcaggccaa caggccgggg cgggtggaga aaaagttccc
      961 tgtcaagcca caagctgaca gggtgagcca aacgacagtc aagccaactt ttaaggagct
     1021 tgaaaatgcg cctcacgacc aaaggccgct ttgcggtcac cgcgatgatt gacttggccc
     1081 tgcgccagaa caacggcccc gttaccctgg ccgccatcag ccagcgccag cagatttcgc
     1141 tgtcctacct tgagcagctg ttcggtaaac tgcgccgcca tgaactcgtc gagtccaccc
     1201 gtggccccgg cggcggttat acgctgggcc gcaaggcggc tgacatcacc gtggccgaca
     1261 tcattgtgtc ggtggacgaa cccatcgact cgacacagtg tggcggcaag gaaaactgtc
     1321 tcggtgacgg tggccgctgc atgacgcacg aattgtgggc ctcgctgaac agccgcatgg
     1381 tggaatttct cgactccgtg accctgcaaa agctggtcga cgaccagctg gccaaagggg
     1441 tggtggttga aaatacaccc atgatgaaga aagccatctt caccgcgccc gtggccaagc
     1501 cgatgcgcgt caatgcgccc aattcggtgt ttgccctggg caacgcgttt tcgaagtcct
     1561 gagcggtcct ggtcttgctg gcctgcgagg gcccgattgc cggagactgc cgtccatttt
     1621 ttgatgctgc cagccagccc tgaaataaaa agctagccac atggaaaccc cacacttccc
     1681 catttacatg gactacagcg ccacgaaccc gtgtgacccg cgggtggtgg acgccatgat
     1741 tccctggttg cgcgagcatt tcggcaatcc cgcttcgcgc agccatgcct ggggctggga
     1801 ggccgaagcc gccgtggaaa aagcccgcga gcaggtggcc gagctgatcg gtgccgaccc
     1861 gcgcgagatc gtctggacca gcggtgcgac cgagtccaac aacctggcgc tcaagggcgc
     1921 cgcgcatttc tacaagtcca aaggcaaaca cctgatcacg gtcaagaccg agcacaaggc
     1981 cgtgctcgac acctgccgcg agctggagcg ccaggggttt gaggtgacct acctcgacgt
     2041 gcaggccgat ggcctgctcg acctggaggt gctgaaggcg gcgatccgcc ccgacaccat
     2101 cattgtcagc gtcatgttcg tcaacaacga gattggcgtc atccaggaca tccccgagat
     2161 tggcgccctg tgccgcgaaa aaggcatcat tttccatgtg gacgcggcgc aggccaccgg
     2221 ccgggtggat atcgatcttg cgaccttgcc ggtcgacctg atgagcctga cctcgcacaa
     2281 gacctacggt cccaaaggca taggcgcgct gtatgtgcgg cgcaagccgc gcgtgcggct
     2341 ggaggcacag atgcacggtg gcggccacga gcgcggcatg cggtccggca ccttgccgac
     2401 gcaccagtgt gtgggcatgg gcgaggccta ccgcattgcc agggcggaaa tgcacgacga
     2461 aaaccagcgt atccgtgccc tgcatgagcg catgctggcg ggtctgaaag acgtggaaga
     2521 ggtcttcctt aatggccatg ccgacaagcg tgtgccgcac aacctcaaca tgagtttcaa
     2581 ctatgtcgag ggtgagtccc tgatcatggg catcaagggc ctggcagtgt ccagtggttc
     2641 ggcctgtact tcggccagcc tcgagcccag ctatgtgctg cgcgccctgg gccgcagcga
     2701 cgaactggcc cacagcagcc tgcgcatgac cataggccgc tggaccaccg aagaagagat
     2761 tgattacgcc gtcgcgacga tcaaggaaaa tgtcgccaaa ctgcgtgacc tgtcgccact
     2821 ctgggaaatg ttcaaggatg gcgtggatct gtccaccatc cagtgggctg ctcactaagc
     2881 agcgcaaaca gcttgaagag gtaaacaaaa tggcatacag cgaaaaagtg gtggaccact
     2941 atgaaaaccc ccgcaacgtc ggctcgtttg aaaagggcga cgacacggta ggcaccggca
     3001 tggtcggtgc accggcctgc ggcgacgtca tgaagctgca gatcaaggtc aacccgctga
     3061 cgggcgtgat tgaagacgcg cgtttcaaga cctacggctg cggctcggcg attgcgtcga
     3121 gttcactcgt gaccgagtgg gtcaagggca agaccctgga cgaagcggca agcatcaaga
     3181 acagcgccat tgccgaagaa ctggccctgc cgccggtgaa aatccattgc tccatcctcg
     3241 ccgaagacgc catcaaggcg gccgtcagcg actacaagct gaagcacgcc cattaacctt
     3301 tattgacctg cgaagctttc catgtccgtt accctcaccg acgccgcagc acgtcacgtc
     3361 acccgctaca tggccaaacg cggcaagggc gtgggtgttc gcctgggcgt cagaaccacc
     3421 ggctgctccg gcctggctta caaactcgaa tatgccgacg agatagcgcc tgaagacgtg
     3481 gtgtttgaag acaacggtgt gaaggtgctg gtggacccga aaagcatggc ttacatcgac
     3541 ggcacccagc tcgacttcgt gcgtgaaggc ctcaatgagg gtttcaagtt catcaatccc
     3601 aatgagcgtg accgctgtgg gtgcggcgaa tcttttcggg tgtgaagcta ctgcgcaggc
     3661 gtgatcgagg cgcgggcagt gctcgccatc ctcacgtacc tgaagtacgt tccggttgct
     3721 gcgctccgtg cgcctagcct gacgcccgct acgcttttca tcacgaaacc agattcttcc
     3781 agtggcgact cttcaatcca ccgatttcga actttttgac gtgcctgtgc agtttgcgca
     3841 ggacagtgcc gttctcgacg cgcgctggaa agagctgcag cgcgaggcgc atcccgaccg
     3901 gtttgcggcg cagggcgctg ccgcccagcg cctggccatg cagtggtcgg tgcgcatcaa
     3961 c
//
LOCUS       DAIDFG010000290         3939 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_290_length_3939_cov_18.110196, whole genome shotgun sequence.
ACCESSION   DAIDFG010000290 DAIDFG010000000
VERSION     DAIDFG010000290.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_290_length_3939_cov_18.110196"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          802 a         1189 c         1213 g          735 t
ORIGIN      
        1 ggccgatggc gcacatctgc tgctcgccgc ccgaggtgta ggctgcctgc gaggtgcgcc
       61 gtgttttcag gcgcggaaaa tagttgtaga ccttctcgag gttggccgcc acttcggcct
      121 tgcttttgcg cgtgtaggcg ccggtcagca ggttttcctc aatcgtcagg tgggcgaagc
      181 agtgccggcc ctccatgacc tgcaccacac cgcgttgcac caggtcggcg ggagacaggt
      241 tctcgatgcg ttcgccgcgc aactcgatcg agcccttggt gaccgcaccg cgttcaccct
      301 gcagcaggtt ggagatggcc cgcagggtcg tggtcttgcc ggcgccattt ccgcccagaa
      361 tggccacgat gcgaccctgc ggcacttgca gggacacgcc cttgagcacc aggatcacgt
      421 ggttgtaaat gacctcgatg ccattgacgt tcaggacgat gtcgctggcg atgtcaggtt
      481 tttccataag aatgtttctg cttgttgctg ttcaaacggc agcgctgcaa caggggcttg
      541 aagcgctggc gtttggcggc tcgctggagc cgcgccccct cccgacgggc gggaggggtg
      601 tttagctgaa gatcaggact ggcagtccgc cggggtgcgc ggcgtgagtt tcttctcggc
      661 ggcatacttg gcggcggtgg ctttgaccat cggtttgatg atctgctcat cggcctgcaa
      721 ccagtcagac gtgaacttcc aggccttgcc gtcccaggtg tggatacggg cccatgccgc
      781 acccatgtgg tccatgcaag aggtgctgat tggccgcatg acgccggcaa aaccgagtgc
      841 gtccagcttg gcctgggtca gattcaggtt ttccagaccc cagcggacct gctcaccact
      901 catgaccttg cccttgccga aacgctcctg ggcggagcga atgccctcga ggcccagcat
      961 ggcactcatg gcaccacgca tgtaaagcac ctggccgact tcgtctttcg gacccgttcc
     1021 ctggcccttg gcatgcacca tggacaggat gtccttgacg accttggagt tgggttccgc
     1081 accgtgctgc atggtcaccg cgttgtagcc cttggcgccg tcggccacgt ccttcacatc
     1141 gggttcagcg ccggaccacc agacgccgta catcttctcg cgcgggtagc cggtggcctg
     1201 ggcttccttg atggcggtgg agttcatcac gccccagccc cacagtgcca catagtcagg
     1261 gcgggcctgg cggatctgca accaggtggc tttctgctcg acgccggggt gcgtcaccgg
     1321 cagcagctgc aggtcaaaac catgcatcct ggagcgttcc tgcaataggg gaatcggctc
     1381 cttgccatag gggctgtcgt ggtagaccag cgctattttt ttgcctttta gtttgtcaaa
     1441 gccaccttcc ttcttggcaa tggcctgcag gatggcgtcg gcggcaaccc agtaggtgcc
     1501 ggcaatcggg aagttccact tgaacaccga tccgtccgcg ctttcgctgc ggccatagcc
     1561 gatggtgacc accgggattt tgtcaatggg cgctttttcc gtgatggcaa aggtggcgcc
     1621 ggtggacagc ggctgcacca gcgaggcccc cttgctcttg agccgttcat aacactccac
     1681 gctgcgagcg gtgtcatagc cggtttcaca ttcttcgaaa gtgatcttca cgccattgag
     1741 accgccgcgc gcattgacca gcttgatgta gtcaacgtag ccgttggccc agggggcacc
     1801 gttcggaccg taagggccgg tgcggtagga cagcaccgga atgaactgct ccttggcctg
     1861 agcgaatgcg ctggtcgcca cgatggacga tgcgccggtg gcaaggactg ccgccgccaa
     1921 tatcaagttg cgaaccttca ttgttgtctc ctagaaatta aaaatacacc cgaaaaaaac
     1981 tggcacccca gtaaacgttc aaacggcaca aaatacatcg gtgcttccca gtaatcctta
     2041 accctgatgg aagctcaatg cgggaacggc cagagccgca tcttctgttt gccgaccgac
     2101 cagagtttgg ccaggccgtg cggctccaca atcaggaacc agacgatcag gccgccgaaa
     2161 atcatcagtt ccgcatgtga cacgcctgcg gtggaaatct cgatgccgaa caggccggca
     2221 aacaccggca ggaactggtt cagcgcgatc ggcagcacca cgatgaaggc ggcgccgaag
     2281 aagctgccca tgatggagcc gaggccaccg atgatgacca tgaacagcag ccggaaggac
     2341 tgatcgacgg agaaggccgc cggttcccag gcaccgaggt aaataaaggc ccacagaccg
     2401 ccggctacgc cgatgatgaa ggagctgacg gcgaaggcgc tgagcttggc gtacatgggc
     2461 cggatgccga tgactgcggc cgccacatcc atgtcgcgga tcgccatcca ttcgcggcca
     2521 atggcgctgc gcaccaggtt cttggccagc agggccacga tggccagcac actcaggcaa
     2581 agcaggtagc ggctcaccga actctcaatg ggtaaaccaa acacctgcag gttggaaacc
     2641 gccactgacc ccgaaggcgt gtccagggtg aaccacttga tgcgcaaaaa catccagtcg
     2701 gaaaaaaatt gcgcggccag tgtggccacc gccaggtaca ggcccttgac acgcaggctg
     2761 ggcaggccga acaggatgcc gaagaaggtg gcacacaggc cccccaggat cagtgccgga
     2821 atcagcggca tgccggggat gcgcacaaag aagttgtagg caccgtaggc gccgaccgcc
     2881 atgaaggcgc cggagcccag cgaaatctga ccgcagtagc cgaccaggat gttcacgccg
     2941 agcgcggcca gcgacatgat cacgaatgga atcaggatgg cgcgaaacag gtagtcgctg
     3001 gccatcatgg ggacggccac gaaagcaatg gccagcagca gcgcgatggc aatgcggtcc
     3061 tgcaggatgg ggaagatctg ctgatctgcc cggtagctgg ttttgaattg gccgttttct
     3121 ctgtagaaca tatttctctt ctgtgctttt tagctacgac acgctacgct taactttgct
     3181 gcgcaaagtg agtcttcgct gaaagatcct cgctgccctt gcgggcaggg aatcaacaag
     3241 ttgattcttc gcgtatttca tacgcgatcg atgatctttt caccgaacag gccttggggc
     3301 cggaacagca ggaagccgag ggccagcacg taggcaaacc agatctcgat gccaccgccc
     3361 acaaacgggc cgaggtagac ctcggagagt ttttcgccga cgccgatgat caggccgcca
     3421 atgatggcgc cgggcaccga ggtcaggccg cccaggatca ccacgggcag ggcgcgcaag
     3481 gccacggtgg ccagtgaaaa ctgcacgccg agcttgctgc cccagatcat gccggccacc
     3541 agcgccacca caccagcgac acaccagacg atgacccaga tgcggttcag tggaatgcca
     3601 atggactgcg ccgcctggtg gtcgtcggcc acggcgcgca gggcccggcc agtggcggtt
     3661 ttctggaaaa agaggctcag cgcggcgacc agcacggcgg cgatcagcgc ggcgtacagg
     3721 tcttccttgt tgatcaggat gccacccggg aagaccgagt cgagcacgaa gaccgggtct
     3781 ttcggcatgc cgatgtcgat cttgtagatg ttgctgccga aaatcgtctg gcccaggcct
     3841 tccatgaagt aggcaatgcc cagggtggcc atcagcaagg tggcgccttc ctggttgacc
     3901 aggtgccgca gcaccaggcg ctcgatgaac caggccaca
//
LOCUS       DAIDFG010000291         3913 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_291_length_3913_cov_19.296008, whole genome shotgun sequence.
ACCESSION   DAIDFG010000291 DAIDFG010000000
VERSION     DAIDFG010000291.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_291_length_3913_cov_19.296008"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          660 a         1241 c         1273 g          739 t
ORIGIN      
        1 gctcgccctg atccgctttc ggctgcgctc ctcggcccag cccgacgggt gggggaaaat
       61 tgcgggttcg ggttcgggtg cggggagaca tgacgcgcaa agcgcgtcat gtcagcgtcg
      121 agcggccgtt atgtttgcac gcgggagcag cacacgcggc caaatgaagt ccccctccat
      181 cgcccagcgg ggcgagggag caaccgtgtt caaagtcaag cgctttttgg tgcccagggc
      241 tcactgtttt tcaccatggc attcatgatg gtgagcaact tgcgcataca cgcaacgatg
      301 actactttgg gcggtttgcc cgcttgcctg agtcgcgtgg cgaacacctt catgcctgga
      361 ttgcagcgca tggcagaaat ggtggccatg tacaagaccg ctcgcacatc ggcccggccg
      421 ccccagacga agcgtttgcc atgatgcttg ccgctatcgt tggccagcgg cgccacaccg
      481 gccaaggcgg cgatctcgcg ccggttgagg gttcccaatt ccgggcactt ggccagcaag
      541 gtcacggtgg tgacggcgcc gacaccggga atgccgcgca acaaatcatc cttggcacgc
      601 cacacgtcag aggcccgcaa gcgccggctc aggtcggcat cgatctcggc aatgcgcttg
      661 tccagccagg caatgtgcgc cttcaagctc ttttgcatcg gtttggttcc ggccgtatcg
      721 agccgcaggg tttcctgcac ccgattggca atcaattgcc ggcgccggct gaccatctcg
      781 tcaaggctcc gggtctgctc atccttcaga ggccgcgcct gtggccggat cgccttggca
      841 aaggctgcca gcatggcagc atcgacccga tcggtcttgg ccaggcgacc ggtggccttg
      901 gcaaagtcgc gtgcctggcg cgggttgatg acggccacgc gaagtccctg cgctgccagc
      961 tcgcaggcca gccgcatctc cagacctccc gtggcttcca tcacgatcaa cgccggctgc
     1021 gccgccgtca ggcgttcgca gacactgcgg atacctttat cgtcgtactc gacatgtatg
     1081 acttcagcgc ttgaatcaat gcaaacgtcc aaagtggact tggacacatc gataccgaca
     1141 aacacctcca cttcagtgtc gttcatacca atacccttcc ttgcaatatg cgggcttgca
     1201 ggcccaggca accgttcggg ctatcgctga aaaacgatgt gacgctcctt gctccctcgc
     1261 gggcttggat atccccaggg gcggacgagc tgccacaccg aaaccatcat cggcctgttc
     1321 attctagatt tctgaacata caaggggcgg ggggagggag tggggagtcg ctcctgctgt
     1381 ccagcgcgaa tcaggaacgc agcgtgatgc cgcaacgcag aggtcagccc ttttgctgca
     1441 tcttctcaat ccgctcgctc atgcgcgaca gcaggtacca ccactcgctg tcgtcgtcgc
     1501 gataggttcg caccgctccg gcgcgcacca ggatcttgcg cagttcgtcg tcgtcgaaac
     1561 cgcccaggtg ttttttgagc acctcgaagg accggtcggt gaagcttttg tggctcagga
     1621 aatggcgcgc ggtttcctcg gccatgaact cggtccggta ctgctgctgc agcgcctgga
     1681 tctggtgctt gaactgctgc cggttcacgg caaaggtcac caggccgcct acgagcgtgc
     1741 ccagcagggt gaacaagggg gtcaggaggg tggggtccat ggcgggctcg cgggtgggtg
     1801 ggtcagaggg gcttgccgga tttgggcagt ccgcattatt gcggtgaatc aaggccccgg
     1861 caggtttcaa cctgtgcgca gcctgcggct tccggatggg actaaactgg tcttgggctc
     1921 acgcgatgcc tgatttttta ccaccgcact tgggagcatg ccatggacac caccagcaaa
     1981 gacaaactga tcaccgacat gaaagtggtc ctcaccgacg tggaggatct gctcaaggcc
     2041 gccaccgccg ccaccggcga gacggcggcc gcgctgcgtg aaaaagctgc gggcaagctc
     2101 aagctcgcca ccgaaaaact gaccggcttg caggaggcgg cgctgctcaa gggcaaggag
     2161 gcggccaagg tcacggacga gtatgtccat gccaacccgt ggagggctgt cggcatcgcc
     2221 gccgcggccg gtttcctgat cggcctgctg gtcaaccgca ggtcatgatt gccatggcgg
     2281 gaagcaccgg cgtgccggtg tgcctgttgt tgccatgaac acaccctctc atgaccgtcc
     2341 ggacggcccc cagccgccgc gcctgttcgc atcgctgcgc cagctgggcc tgacggcgct
     2401 gtccatggcg cacacgcgcg tggcgctggc aggcgtcgag ctcgaggaag agttgcagcg
     2461 gctgatcggc ctgttgctga gcctgctggg cctgctcgtg ttcggtctgg tcggcgtcct
     2521 gatggtcacc ttgatggtgg tgctggccgt ggaccctgcg cagcgtgttg cagtgctggc
     2581 gttttttgcg gcggtgtacc tgggcctggg cggctggttc tggtggcgcg tgcagcgcac
     2641 gctggccacg cggccgccct tcctgcaggc cacctttgcc gaaatggcgc aggaccgcaa
     2701 tgccctgcaa gccgccgcga ccagcgccat gccgcagggc agagaccccc atggataacg
     2761 ccacccccca gagccagcgg gcgctgagaa agcagttgct gttgatgcgc tcggcaacgg
     2821 agcgcgccga actggtccag gctctcggtg cggcgcggca gtcggtgacg atgttcaaag
     2881 gcggtatccc gggaggggtg ttcggtaaag gcttgccgct ggcgctgggc ctgctcaagc
     2941 gtgcgcccct gctcaccccg gtgttgtcgc tggtgctggc cggcgcgcgc cggccgctgc
     3001 tgcgttacgc cttgctcggt gcgggttcgg ccctgctggt gtggaagggt taccagtggc
     3061 tggcagatca gcgcccgcct gcgaccgggc cggcagacga gggcgatcca ccgcctgcct
     3121 cctgatcttg cgcctgcgcg gcagcgtgcg cgttcaccgg gcctttgatg ctcctgaata
     3181 gatagctgct tgcgctcgtg tctattgggg cggaggcatt tttctcttaa aagaagcgga
     3241 ctgacctcaa ctcagatttc caggttgtca atcagccgcg tggtgcccag tctggccgcg
     3301 cccagcacca ccagcggctc cgctgcctcg ctgcttccgg gcggcaacag gtcggtgcga
     3361 cggcgcacgg tcaggtagtc gggggcccag ccgcgcgcgc tcagggcctg catcgcctgg
     3421 gcttccagga catcgagcgt gtccagacct tgcgccctgg cggcctcgcc cagcgccttc
     3481 aaggccctgg acagatacac ggcttcggca cgctgcgcgg catccagata gccgttgcgc
     3541 gaggacagcg ccaggccgtc cgcggcgcgc agggtttcac cggggacgat cgctatcggc
     3601 agggcaaact gctgcaccat gcgccggatg atcatgacct gctggtagtc ttttttgccg
     3661 aacagcgcgg tgcccccgga cttgccggca aacacgcagg aaaacagctt catcaccacg
     3721 gtgctgacac cgacaaaaaa gccggggcgg aaatgccctt cgaggatgtc ggccagttcg
     3781 gccggcggat gcaccttgta ggtctgcggc tcgggataga ggtcgtgctc ccgcggcgca
     3841 aacagcacat cgcagccggc ggcctgcagt ttctcgcagt cggcttccca agtgcgggga
     3901 taggtgtcga aat
//
LOCUS       DAIDFG010000292         3899 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_292_length_3899_cov_20.046046, whole genome shotgun sequence.
ACCESSION   DAIDFG010000292 DAIDFG010000000
VERSION     DAIDFG010000292.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_292_length_3899_cov_20.046046"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          670 a         1311 c         1290 g          628 t
ORIGIN      
        1 catcgaggcc tgcggcctgg cgcccagcaa tattgccggt gcgcttgggg cgcgtgccgg
       61 cttgttcgaa tgcggcgagg tgcgggtgca cccgaccggc agcgtgacgg tgttcaccgg
      121 ttcgcacagc catggacaag gccatgagac cacctttgcg caggtggtgg cggcgcggct
      181 cggcatttcg gtggaccagg tcgatgtggt gcatggcgac accgggcgcg ttcctttcgg
      241 catgggcacc tatggttcac gttcgctgtc ggtcggcgga acggccatca tgaaggcgct
      301 cgacaagatc gagaccaagg ccaagaagat tgccgcgcac ctgatggaag ccagcgatgc
      361 cgacgtcgag tttgccaacg gcgagttcac catcaagggc accgacaaga aggtgacctt
      421 cggccaggtg gcgctgacgg cctatgtgcc acacaactac ccgctcgaca agctcgaacc
      481 cggcctgaac gaaaccgcgt tttacgaccc gaccaacttc accttccctg ccggcaccta
      541 catctgcgag gtcgaggtgg acccggccac cggccaggtg cgcgtggacc gcttcaccgc
      601 ggtggacgat ttcggcaaca tcatcaaccc gatgattgtc gaaggccagg tgcatggcgg
      661 gctggtgcag ggcatcggcc aggcgctgct ggagaactgc gtgtacgacc ccgagtccgg
      721 ccaactgctg actggctcct tcatggacta caccatgccg cgtgccgatg acatgcccaa
      781 cttcacgctg gacaccttgt gcacgccgtg cagccacaac ccgctgggcg ccaagggctg
      841 cggcgaggcc ggtgccatcg gttcgccgcc ggcggtcatc aatgccgtgc tcgacgccct
      901 caagccgctc ggcgtgactg acttcgacat gccagcctct cccggccgcg tctgggaagc
      961 catccagtcg gccaagaaat aaacagctat ccaaggagaa acccaccatg tacccattca
     1021 ccctggaacg tcccacctcc ctggctgccg ccgcgcagct cacggccgac ggcgccaaag
     1081 ccctggccgg cggccagacc ctgttggcgt cgatgaaact gcgcctgtcc agcccggacc
     1141 agctggtgga cctcagcggc atcaaggagc tgaccagcat caagcgcgag ggcaatgcct
     1201 ttgtgatcgg cgccatgacg cgccacgccg acgtggcgac caacgccgag ctgaaggcag
     1261 cgctgcccgc gctcgccgac ctggccgcca acatcggcga ccggcaggtc cgcgccatgg
     1321 gaacgctcgg cggctcggtc gccaacaacg acccttcggc ctgctacccg agtgcggtgc
     1381 tggcgctggg cgcgaccatc cacaccacca agcgcaagat tgccgcagac gactttttcc
     1441 tcggcatgtt cgccaccgcg ctggaagagg gcgagctgat caccgcgatc agcttcccga
     1501 ttccgaagcg cgcggcctac atgaagttca agcagtcggc ctcgcgtttt gcgctggttg
     1561 gcgtgtttgt ggcgcagttc gatgccggcg tgcgtgtggc cgtcacgggc gcctccagcg
     1621 gcgtgttccg tcacgcgggg ctggaagcgg cgctgagcaa gagcttcaca cccgaagcgg
     1681 ccgcggcggt gaaaattgac gcgacggacc tcaacagcga catccacgcg acggcggctt
     1741 accgcgccaa cctgatcagc gtgcaggccc agcgcggcgt ggcgaaggcg ctggcctgac
     1801 agtgtccaac cgtccggact gggcccgccc cgggcccggt ctgaacggtt tgaagccttt
     1861 gatgtctttt aggcctcccg cccttgatgg gtgggcgcaa gcagctattg aatagatagc
     1921 aacaggtaca ctgatccgat gacacctttt cccacgattg atgcactggc ccaggcgctg
     1981 cagggcgccg gctactttgc cgaccggcgg ctggccacgg cggtgtttct cgcgctcaag
     2041 ctgcagcgcc cgctgctgct cgaaggcgag cccggcgtcg gcaagaccga gttggccaag
     2101 gcgctgtcgc tggcgctgca gcgccagctg atccgcctgc agtgttacga cgggctggag
     2161 cagcgcgaag cgctctacga gtggaattac gccgcccagt tattgcacat gcgtgctgtg
     2221 gaaggccatg gtgacgcggc cgatgtcgaa cgcgaggttt accaggaccg ctacctgata
     2281 cgccggccgc tgctgcaggc gctgcagacc ccggcgccgg gtgcggtgct gctgatcgac
     2341 gaggtggacc gcgccgacga gccttttgaa gcctttctgc tcgagtacct cggcgagtac
     2401 caggtcagca ttccggaaat cggcacggtg cgtgcgcagg tgacgccggt caccatattg
     2461 accagcaacc gcacgcgcga actcaacgac gcggtcaagc ggcgctgcct ttaccactgg
     2521 ctcgattacc ccgaccgcga ccgtgagctg gccatcattc gcgcccaggt gccggaggcg
     2581 ggcgaacagc tgtcagccca ggtggccggt tttgtcgacc ggctgcgccg ctcgccgttt
     2641 ggcaacgcct tccagcgcgc gcccggcatt gccgaaagcg tcgagtgggc caaggcgctg
     2701 gtggcgctgg acacgctggt cgtggacccg gaagtggtgg ccgacaccgc gggtattctg
     2761 ttcaagcagc gcgaagacgt ggcggcgctc acgcatgagc tggcggtgga attgctcaag
     2821 cccgaagagg cggccgccga gtgaagcggg cgaggtagtc catgttattg ggtgacgcca
     2881 gagcgggcaa actcgccgac aacatcgccg gcttcggccg cgccctgcgc cgcgcgggtg
     2941 tcaaggtgga cagccagcgc attgccctgg ccaaggaagc cgcactcgcc gtcggcatca
     3001 gcgaaaggga agacctgagc gctgcttttg aagccgtgat ggtcagccgc gagcaggacc
     3061 ggctggtctt ccgcgaactg ttcgacgtgt attttcagga cccgaaagtc gccaacaagc
     3121 tgctcgcgca actgctgccc agtgccgagg gcaaggccga gcccagcaag cgccgcccgc
     3181 gtgtgcgcga ggcgctggcg ccgcaaaaag cgtttggcca gccggcaaaa ccgacgacgg
     3241 aagaccagaa ggtcgagttc gacgccgcca tgactgccag cgacatcgag cgtctgcagc
     3301 atgccgactt caatgcgctg tcggccaccg agtaccggct ggtcgagcgg ctggcgcgcg
     3361 acatcaagct gcccttgccg atgtttgcct cgcggcgcag ccggccggcc gccagtggcg
     3421 cgcgcatgca ctggccgggc gtgatgcacc aggccgcacg caccggcggc gagttgatgc
     3481 acctgccgcg gctgcagcgg cgcgagcagg ccctgccttt gctggtgctg gtcgatgtgt
     3541 cggggtcgat ggagcgttat gcgcgcctgc tgctggcctt tctgcacgcc gccacacggc
     3601 gccacccgca tcgcgatgtg ttcgccttcg gcatgcacct gaccgatctg acgcaggcct
     3661 tccgccaggc cgacaccgac gagatgctgg cccacgccag cctggcgatc gaagactttg
     3721 ccggcggcac ccagctcggc gcgtcgctgg ccaccttgcg cgaacgccat gcgcgccgcc
     3781 tggtcggccg gcgcaccatc gtgctggtca tcaccgacgg gctggacacc ggcgagccgg
     3841 ccgacctggc ccgcgaactg gcttggctac gccggcgcag ccgccgcctg ctgtggctc
//
LOCUS       DAIDFG010000293         3892 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_293_length_3892_cov_19.044305, whole genome shotgun sequence.
ACCESSION   DAIDFG010000293 DAIDFG010000000
VERSION     DAIDFG010000293.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_293_length_3892_cov_19.044305"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          619 a         1267 c         1255 g          751 t
ORIGIN      
        1 gcgcagcaca cgcggccaaa tgaagtcccc ctccatcgcc cagcgaggcg agggacggga
       61 taggggaggg agtggggagt cgctcctact gccttgggcg aaccgcagtc gcggcgtgaa
      121 gccggcgatc cccgggtgcc aggacgccag agatgcaatg ccctcagcgc agccccgcac
      181 cacccgtatc gttcttgcgc tcaatcaact cgatcttgta gccatccgga tcggtgacaa
      241 acgcaatcac ggttgtgccg cccttgaccg gcccggcctc gcgcgtcacg ttgccgccag
      301 ctgccttgat tttttcgcag gccgcgtagg cgtcgggaac gcccagtgca atatgaccat
      361 aggcggtgcc catctcgtag ctgtcggtgt cccagttcca ggtcagttcg atctcggcct
      421 ggcccggatt gccgccctcg tagccgacaa aggccaggct gtatttgtat tccgggtttt
      481 ccgacgtgcg cagcagcgtc atgccgagca cctgggtgta gaaatcaatc gagcgttgca
      541 gcttgccaac gcgcagcatg gtgtgcagga gtctcatcgt gtttctcttc cttttgaaca
      601 ggtgttggca ttgtccggca atccagaacc ccccaacaca acgggggaaa ttcacggttt
      661 tccgggcgat atactggcca gctatttacg cagcgcccct cattcatgcc cctgtcttcc
      721 acgactgatc agcctcatga ctggctgtgc tccacccgcc tgctcgatct tgatgatccc
      781 aaactccgta tccaggccat gcgcatcacg cagctggcga ccacgccaac ccggaaggcc
      841 gtggcccttc atgacttcgt caagtcgctg ccttttggtt gcgtggccgg ttttgaccat
      901 gtaccggcgg cggccgtgct caaggcggga cgcggcgact gccacaccaa gggcacgctg
      961 tttgtagcca tgctgcgttc ggtcggcgtg cccgcccggc tgcgtttcgt gaccctctcc
     1021 agcgggtttc tcaagggcat catcgatgtg ccgcagagca ccatcaccca tgccgtggcg
     1081 gaggtttacc tggacgcgcg ctggatacag accgacacct atgtgacgga ctccctgctg
     1141 gaggcgcagg ctgcagagtt gctgagccag accggctgtc tggtgggcta cggcatccac
     1201 gtccggggca tgcatttctg ggatggccgg catgccgcgc atgcgcagta caacgtttcc
     1261 gacccggcca gcctgccgct gcatgactgg ggcgtggcgc acgatcccga gcatttctac
     1321 agcagcaagg accatgcgga gctggagctc ggctggctga cgcgggccaa atggatgctc
     1381 gccgccgcgc tggtgaaccg gcgtgtggag caggtccgga cccagcccgg ggcggcaggg
     1441 cagcccgcct aggaatacct tcccgtccga agttcaggcc gcttttggcg gcggcacctc
     1501 aaacaccagg caggtggtgg tggcgtgggc atacagcttg ccgtccggcc cgatgatgcg
     1561 ggcttcggcc gtggccattt gccggccgca atggatgacc gtgccgatcg cacgcagcgg
     1621 tccggtttta tgggacgcgg cccgcacgat gttgacgccg agttccgccg tggtgtaggc
     1681 gcggccggcc ggcatcatgg tgtgcacagc gcagcccagc gccgagtcca gcagcgtggc
     1741 gtaccagccg ccatgcaccg tgcccagcgg gttgtaatgc ttgagctgcg gcgtgccctg
     1801 gaacaccgct ttgccggggc cgacctccac cagcgaaaaa tcgagtgtgt cggcaatgtg
     1861 cggaaagggc agctcgccgg ccaacatggc ctgcatgatc tccagcccgc tcttgccggc
     1921 caccatggac ggatgggcca ggcccggttt gccgccgccg gccagcatgc gctcgacgac
     1981 ggcgttgcgc tcctcgttcc ataacgccaa tgtttcttcc ggggtcattg tttctccttt
     2041 gattgcacct gcaataattg tagctacaat tatcacatgt ccagaaaaac cgctgccgcc
     2101 acccaggccg acctgaaagc cctgggttgc accaacctga agctgcgcca gctgatgcgg
     2161 cgtgtcgcca cctattacga cctcgagatg gcgaagtcgg gcctgaagac cacccagtat
     2221 tcgctgctgt cccatgttct caagctcggc cccctgcgtc ccggcgagct ggcgcaaagc
     2281 atgaaggtca gcgcctccac cctgacgcgc aatctcaaac ccctgatcga tgccggctgg
     2341 atcgaactga ccgctggcac cgatgcacgc agccgcagtg tggccatcac accggcgggc
     2401 cgtgccaaac gcgaggaggc ccgccatcgc tggaaggtgg cgcaggaggg cttgaaccac
     2461 ctgctgggcg cgcagcgcgt gctggcgctg catggcctga tcaatgagtc gctggagctg
     2521 ctgtcccccg ttgaaaccgg atccggagcc gatgatgaat aaaccttcct cttcctcgtc
     2581 tgccaggcgg catgtcgcgc tgtggctggt gctgctggcc gcggccggca cctttgcgct
     2641 gaccatgggc gtgcgacaga ccatgggcct gttcctgtcg gcgctcaata cctcgaccgc
     2701 gctgggcata ggcagcatca gcctggcctt tgcgtttggc cagttgtggt gggggttgac
     2761 ccagcctttt gccggcgccg tggccgaccg catcggcacc ggccgcgtga tttttctggg
     2821 cgtgttgctg gtggccacag gcaccatcct cacgccgctg atgaccagca cggcgggcct
     2881 gatatttgcc attggtgtgc tcgccgcggg tggtgcgggc atggctggcc cgtcggtgct
     2941 gatggcggcg accgcacgcc tggtgccgct ggaaaaacgc ggctttgcca gcggcatcgt
     3001 caatgcgggc ggctccttcg gccagtttgc gatggcgccg atcgcgattg gcctgacggc
     3061 gaccgtcggc tgggccaacg cgatgcagtg gttgggcgtg ctgatcctgc tggcgctgcc
     3121 ggcggtgtgg gtgctccagg gcaactcgaa cgccctggca gcacaggccg cggcagcctc
     3181 cggcaagaaa gcactgagcg cccgccaggc gattcgccag gcgttggcca cgcccagtta
     3241 ccgatacctg agcctggggt ttttcgtctg cggattccac gtcgcctttc tggccacgca
     3301 cctgccgggt gtggtggctg cctgcggcct gccgccggag atcggcggct gggcactggc
     3361 catgatcggc ctgttcaaca tcgtcggcag cctcgccatg ggctgggccg tgggccgttg
     3421 gcgcatgaag tcgctactgg cgctgctgta cgcgacgcgc ggcatcgcgg tactgatctt
     3481 tttgctggcg ccgaaaacac cggcggtgat gctggtgttt gccgccgtca tgggcgtgac
     3541 ctttctctcg acggttccgc cgaccgcggg cctggtggcc aagatgttcg gcacggccaa
     3601 catggccatg ctgttcggca tcgtgatgct ggcgcaccag atcggcggct ttttcggcgc
     3661 cttcctcggt ggttatgtct ttgaagccac cggcagctac gactgggtct ggtacatcga
     3721 catcgtgctg gccgcgggtg ccgccctcgt gaacctgccg atccgtgagg ccaagttgcc
     3781 gcgtgcaagc agggctgcaa catgaacagc gccaatgcgt cgagtacagc ctcttcaagt
     3841 caagcagggg ccgcggaacc ggctctgccg ggccgcaggc gcagcgcccc ct
//
LOCUS       DAIDFG010000294         3888 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_294_length_3888_cov_19.580485, whole genome shotgun sequence.
ACCESSION   DAIDFG010000294 DAIDFG010000000
VERSION     DAIDFG010000294.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_294_length_3888_cov_19.580485"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          663 a         1143 c         1320 g          762 t
ORIGIN      
        1 tgtgcagggt gccggtcaca ttgggcggcg gcagctggat ggaaaacgag ggcttggccg
       61 cgtccagcgt cggttcgtac atgccgctgc cttcccagag cggggcccac tggcgttcaa
      121 tcgctgcggg ttcgaaggac ttggccaggc tgtccaggcc gggcgtgggc acggtcaccc
      181 ggggtgttgg ggtgtcggca ggagcggggc tggcgggcgt gggggtggca gaggcggtca
      241 tggggcaatg aaaaacggca tcgcctagtg cagtgttgca tgctggatgg aacaaataaa
      301 gatgatggat tttgggtcag ggctaggcgc aaaccacagc gataccccgg gtatcgcgcg
      361 gatttgcaac gccgccatgg cgcaaaagac gcatttttat gttccagata gcgtgcaaca
      421 ctgcactaga tgccgccttg ggttggaggt actgccccga ttttattcga ctcgctgcac
      481 cggcggggcc ggggccctca ttgagcccgc gccgcgtcag gcggtgtcga tctgcctggc
      541 cgcggcgata caggcctgta gcagcggcgt gtcgcccagc tggttggcca ggatcaggat
      601 caaccgggcg ttgagcagtt cggactgctc gcggctcagg ccctggtggg cgtcgagcag
      661 gcattcgtaa aacgcatccg catcctggaa attgggggcg gtcttgatgg cggcttcggt
      721 tttcatggga gatctttccg gattcagcgc aggccgaggg ttttttcgac agccgcgacc
      781 aattggccgt ccacggcctc gccagtggcg gccaggtagc cgtcgggccg caccagggcc
      841 caggcgtggc caaacacatg gcaggcgccc tgcaaatggc cggtgccccg ggcattgatg
      901 tcgcgcacat gttcgcgcgc ctgcgcgcgg tcgtccgggc ccagcacctg cacgctgcgc
      961 atcggggcgt gcccggcgag ctgccgcagg cgctgcaggc cggcggccgg cacttcgccg
     1021 aacaccagca ccagcaggtc gccgtctgcc cactgcagca ggtcgttgac agcaccttgt
     1081 gaaccatctg cccagcggaa ggccacgttc tgcaccgact gcccgccggt gttgtcgcag
     1141 atggacgaac gtgtgtaggt gttggccacc gccatgcggc cggtgttcac cagcgagcgc
     1201 gcaaacgggt actgcctggc caggccgatg gccgccgtgc gaaagcagcg ctccatgccg
     1261 tctgccggtc gcaggaagcg tgcggtgcgg ttggtcacct gcacgttctg ctgggcggct
     1321 tcgagccgct ccgcgttgta gctctccagc agtgtcgcgc tggcacggcc gcgcagcacg
     1381 gcggccagct tccaggcgag attgtcggca tcggccacgc cggtgttgcc accgcgcgcg
     1441 ccgaaggggc tgactatttt tgcggtgtcg cccatgaaaa acacgcggcc cacacgcagc
     1501 ttgtccaggc agcggctgcg gtaggcgtag ggccccaccc agacgatctc gacctcgacg
     1561 tccttgccga actggcgctg caggcgttcg cgcaccacgt cttcgcggct cacgtcgtcg
     1621 ggatcggcat tgggcgccat ctggtagtcg atgcgccaga cgtcgtcggc catcaggtgc
     1681 tgccagaccg cgcggttttc gttgaacggc gcctcgatcc aggtgtggcg ttcgaccggc
     1741 ggatgtttgg aaaagcgcac gtcggcgatg caccagcggt catcgccgcg tttgttctgc
     1801 atggtggcgc ccacccagtc gtgaaagggc gtgtgtgagc cggaagcatc gatcacatgc
     1861 tcggcctcga tctggtagct gcccgcaggt gtttccaccg tcagcgtggc gacatcggcg
     1921 ttctgcgtga aggcggtgac acgcgacttc cagcgcaggt ccacatggcc gagttcgtag
     1981 atgcgttcga ccaggtagcc ctcgatgtag aactgctgga tgttgatgaa gggcggctgg
     2041 gtcgagaggt tgaagttgct ctgctggcgc aggtcgaagg aatagacctc gtcgtcaccc
     2101 gcaaaggtgc ggcccacgct ccactgcacg cccttggcgg caatacgctc gtagatgccg
     2161 aggcggtgaa agacctccag cgacttctgc gtgtagcaga tgccgcgcga cgaggcgccc
     2221 ttgacgccca cggtgttgtc ttcatccagc agcacggcct cgacgccata gcgtgccagc
     2281 gcgcaggcca ggctcaggcc ggtaatgccg ccgccgacga tgacgatggg atggcgcttg
     2341 atttgttttg agttgagctc gggcggagcc acgaaagggt attcaggcag ggcgtaaccc
     2401 gtgccctggg tgaactcata accgttggtg aacgccgtct gggcgtaggc aggtggtgtc
     2461 tccagcatgg cgtgtctcgc tcccggcatt gatctgtgtt cagtgttttt cgggcctggc
     2521 ccttgtcggt cgggcgcagg cagctatcaa aataatagta agcagggcgc gtcaggcggt
     2581 ggctttgccg ccgaactccg gcctgtcgtt ttgctgcggt ttgagggctt cggctgcgat
     2641 gccggccgcc ttgtccttgc gccactgctg cttgcgtgcc gtccattccg ccaggcgggc
     2701 gtgggcggtc ttgctttcct ccagcatctc gaactcgtcg gccagttgcg ccgtcagctc
     2761 cagacgcacg ccgttcgggt cgaagaaata aatgctcttg aagatgtggt ggtcggtcac
     2821 gcccagcact tcgatgccgt cggcttcgag gcgcgctttc atcgcttcca ggtcggccac
     2881 ggtgtcgacg cgaaacgaga tgtggttgac ccacagcggc gtgttcggcg agggcagggc
     2941 cgcctcgtca ttgccgaggt cgaaaaaagc gatgaaggag ccgtccttca ggcggaagaa
     3001 gaagtgggtg taggggcagt actcgccggt gctcggaacg tagtcgctct ggatgatgtg
     3061 gtacagcggc aggccgagga tgtcctcgta gaaatgccgg gtttcctcgg cgtctttggc
     3121 gcggtaggcg tagtggtgca gttgctgcac cgccgcgggc gccggcaacg gcgaggtggc
     3181 ggggcgcagg gtgggggcgg tcatctgggc agccatggcg atctccggat tgggtgggtt
     3241 cgggtgtttg cgtgccttgg gtgagcaagg cttcaaattc acctattcat aatgcgttta
     3301 ctataacgta actcttcgga cctgccaagt gacagcctgt cggttggcat gaagttgttg
     3361 agaataatta tcaattgcaa ttgatccata gcttttttct tttgttcaaa ttgatcctgg
     3421 caattagacg cagcacctta atgggactat cgtgttctcg gccttgacga tggcgttcag
     3481 ggcaagcaca gtttttacca accacgaagg agatttccat ggctgctctc aaaggctcca
     3541 ggacggaaga aaacctgaag gccgcatttg ccggcgaatc ccaggccaac cgccgttatc
     3601 tgtatttcgc gaacaaggcc gacgtcgaag gccaacccga tgtcgcagcg ctgttccgct
     3661 ccaccgcgga aggcgaaacc ggccacgcgc acggtcacct cgaatggctg gagcagtgcg
     3721 gcgacccggc cactggcatg ccttttggcg gcacgcgcga gaacctcgcc tcggctgtgg
     3781 ccggcgagac ccacgagtac accgacatgt accccggcat ggccaaaacc gcacgtgacg
     3841 aaggtcacga cgaagtggcg gactggttcg agacgctggc caaagccg
//
LOCUS       DAIDFG010000295         3882 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_295_length_3882_cov_19.275412, whole genome shotgun sequence.
ACCESSION   DAIDFG010000295 DAIDFG010000000
VERSION     DAIDFG010000295.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_295_length_3882_cov_19.275412"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          767 a         1176 c         1183 g          756 t
ORIGIN      
        1 atcacgagcg cgaccgcgct ttacggaaag cacccctcca tggcctctgt tgctccagct
       61 cttccatctg ccgcgtcacc ccggcacatc ctgatcgtgg aaaacatcgc ggcccaggcc
      121 cagatgctga gccatatcct cgaagaagag ggttaccagg tcagtgttgt tgacaacggt
      181 cagtccgcgc tggaaatgac gccccggctc cgtccctcgc tgatcatcag cggggtggcc
      241 attccagaga tgagcggcta tgagctgtgc cggcagatca aggccggccc cgggctttcc
      301 ccggtcccgg tgattctcgt caccagtctg tccgacccca acgaggttct gctcggcctg
      361 cagtgtgggg ccgacagctt cgtcatcaag ccctacgaga gaagccacat gctggcgcgg
      421 gtggagcacg ccctgagcaa ccgcgacatc acccacatgc cggaagaagg cccgccggcc
      481 gagatttttt tccacggcga gcggcacgag atcagcgcca gccgcaccca gatactgaac
      541 ctgctgatgt ccacctacga cgctgccatg cagcgcaaca acgaactcca cgaaagccgg
      601 cagcagctgc gcgaacgcac ggccgaggtg ctggaggcca accgttttct cgactcgatg
      661 atcgagcaca ttccgacgcc tgtgtacatc aaggatgccg atcagctgcg ttatgtgcgt
      721 gtcaactctg cgcaggagca actgacgggc atcttgcgcg aggacgcgct gggaaaatcc
      781 gaccacgatc tattcggtca ggaggacgcc gaccggtttt ccgcccagga ccaccgggtg
      841 ctggccagcg gcatcattga ggacattccg gaagaccggt ttttctcaca gagcaaaggc
      901 atgcgcctgc tgcacacccg caaggtgccg gttttcggcg aaggcgccga gcccacgcat
      961 gtgctgggga tttccgagga catcaccctg aagaaggaac tggaaaaggt gatcgccgat
     1021 cttcattccg ccctgaaggt gcgcgccggc gagctggaaa ccacgaacaa aagcctggag
     1081 agtttcacct cggccgcctc gcatgacctg cgttccccgc tgagcgtcat tggcggttac
     1141 gtgagcctgc tggagaaaaa ttacgccgac cgtctggacg aaaaaggctt gcactacctg
     1201 tcggtcatcg gaatccgggt caaaagcatg gccaggctga ttgacgacct gctgtctttt
     1261 tccaggctca gcctgcagga ggtccagaaa accgatgtcg acatgaacgg actggccgcg
     1321 caggtggcag ccgagctgcc agtcagcggc ccgggggcga aaaagcctgt catccagttc
     1381 ggtttgttgc catcagcgcc tgccgacccc gggctgcttc ggcaggtctg ggtgaacctg
     1441 ctgtccaacg cggtgaagta cagcagcctg acggccaatc cgcagatcga ggtcagtgga
     1501 cgcgtcgaag gcgcggaagc cgtctacacc gtgcgtgaca acggcgccgg gttcgacatg
     1561 gcccattacg acaagctctt cgaagtgttc gagcggctgc acaccgacca ggagttcgaa
     1621 ggcaccggcg tgggcctggc caccgtgcgc cgtgtggtca cgcggcatgg cgggcgggtg
     1681 tgggcggaag gcaaggtgga ccagggggcg gtgtttcatt ttgcattgcc ggtgcagagc
     1741 tgctgacgca gacgtgactg gaaagagggg gggctccttc gtttggggga ggcgccccaa
     1801 gcgcggtgcg gcctaaaatc acgaggtctg ctgcgggggt ctgctgcggg ttttacgcct
     1861 tgttttgtgt atctgtatga tttgcccgcc cgttcttcgc aaaaaccggc aggacgccac
     1921 tttcggtggc gtttcgtgcc caggtcaaac cgcagccggc aagttagccg caggttttgc
     1981 ggcatacgtt cgttgcacct caaaccaatc cccagaagga attttttgaa tatgaaatcc
     2041 atgatgaagt tctgcgcctg catcatcgcc tgtacatccg ctttcgccgc gtccgcaagg
     2101 gacagcggct gcctgattca aggaacactt acctcgaaca agatatccca gacctcgaat
     2161 tattgtgcgg ccaacaaggg gatgagcgat caggacttca aggccggctg tcaggacttg
     2221 ttcgagtcac aagggggaaa ttcagacggc aagaaatcgg acaacagtat ttcaatgaaa
     2281 tttgtcagcg attgcccggc caattccaaa ggcgtttgtg caggagcctt tgaccataaa
     2341 tggaacttgc aatacatggc ggacgactat gcgttgaagc acggccaggc caagcaggtt
     2401 tgcgaagtga gcggcggcaa gtggaagcag taatccgatt cgcgcgatga cgctcaggca
     2461 ttgttgccga ggccgccgac aagcctggct cgccgcctga ttccccaacc agggacatga
     2521 ccccgatgtc caccgggtcg cccccccgcg ggtcgcgcgc tgtttgactt ccagagccat
     2581 gacgaggttt ttgcatgcac accttcagca ccagcatcga tattgcgcag cccgtcgaac
     2641 gcgcctggtc cgtgatgatc gatgtggacc actggcatga atggacgcct tcgatcacga
     2701 gcgtccgcag gctgggtggc cagcccttgg ccgtgggcag ccgtgtgctg attcggcagc
     2761 ccatgtttcc gccggccctg tgggccgtgt cggcgctggt gcctggggag cgcttttcct
     2821 gggtcagcgt ggcccccggc ctgcgcgtga ccggacatca cgacgtggtg ccgatttccg
     2881 gcggaagccg ggcgacgctg ggaatcgaga tcgatggcct ttgcggcggg ctctgggccc
     2941 gcttgacgcg ggcaataagc gagcgttacc tcggttatga agcgcaagga ctcaaggccc
     3001 gcgccgggga tggggcgtac cggcacggag gtcttccccg ttcgccgcat cgatgacacc
     3061 tgaagcccct tcatcaccct tgctgcaagc tgcgacttcc gccatcaccc cgggacggcg
     3121 aagtccaaca cggcattgca gtggccccgc cttgctcacc gctgaatttc tacggtttct
     3181 gccattgcgg tagtccgatc atccggcacg ttcttcccac cacacccttc tgggatcgtc
     3241 ctccgcatga gcggcaacac cttcggcatc gtccatcgac aagccgatga gtcagcgata
     3301 caacagcttg tattgcgtct gctccatcaa ctgacgctcc gggcgaatgg agtacagcac
     3361 ctgcagcagc atggcgtgca gcagcttctc aggcgcggtg ctcgggcggc cgcccttgac
     3421 gtcgtcttcg tacattttgc gaacaactca cccattccgg ccgaagcctt gttgactatc
     3481 gtgcggatga cccgcagcag gtgattggct ggcacgaagt catccagctt cttgagggtg
     3541 aacagacctt cggtgaatgt gtcggcgccg cgcagagttg tgtcagttcg gattcaatgt
     3601 ggtgctacgc tgggagcgct tcatgcggtg tggactgggt cggtaggtat ttctgcagcc
     3661 tgtcagacct cagatccatc caactcggaa cgatgcaagg ccagacgtgt ttggttacat
     3721 ctagagtctc tgcaatcgaa tccgcaagca cagtcatctc gacccgctga gctcgctggc
     3781 attcgagcag cttcgcactg gacgcggaaa tgtggcgacg aaagcgggtc aggtccacag
     3841 cgccgtagag ccagctccgt ccgcaggccc gttgtcccaa gc
//
LOCUS       DAIDFG010000296         3823 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_296_length_3823_cov_18.884820, whole genome shotgun sequence.
ACCESSION   DAIDFG010000296 DAIDFG010000000
VERSION     DAIDFG010000296.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_296_length_3823_cov_18.884820"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          745 a         1232 c         1157 g          689 t
ORIGIN      
        1 gccgacaaac atctcgacga agtcagaacc ggaaatcgag aaaaacggaa ccttggcttc
       61 gccggcaatg cctttggcga gcagggtctt gccggtgccc ggtgggccga ccagcaacag
      121 gccgcgcgga atgcgtccgc ccagcttctg gaatttttgc gggtctttca ggaaatcgac
      181 cacttccttg acctcttcct tggcctcgtc gcagccggca acgtcggcaa aggtcacggt
      241 attggtggac tcgtcgagca tgcgggcctt ggatttgccg aagctgaaag ccccgccctt
      301 gccgccgccc tgcatttgcc gcatgaaata gatccagaca ccgatcagca gcagcatcgg
      361 accccaactg accagcaggg tcatcaggag cgagccttct tcacgcggct tgacatcgaa
      421 cttgacatcg ttggcgatca ggtcgccgac cagaccgcgg tccaggtagg tggccgtggt
      481 gcggatgcgg cggtcatcgg tcgtgacggc cgaaatttcc gtgccgccct ggccctccgc
      541 gatggtcgcg gacttgatgc gtttgccctt gacctcgtcg aggaactccg aatagccaag
      601 gtaattcgta ccggcgccac cgcgtgtatc aaattgcttg aaaacggtga acagcaccat
      661 ggcgatcacc agccagattg caatttttga aaaccactga ttgttcaagg atgtctccga
      721 tagaggaaca caaggcacat gcgcctgatt ttaggcgttt caaggccaaa agccgcttaa
      781 caagcccggg acatcccttg gtttgaagcg ggattgcggt atttattgcg tttcagcaag
      841 ctcaggccgt ttgggccggc ctgtttttca gccccatgcc gaccagaaag gtttccgacg
      901 actccgagcg cgaggccttg ggcttgatgg gcttgaccac cttgaaggtc tccttgaaca
      961 gcttgaccag gggactgtag gcgccgccat ggaacagctt gacgaccagc gcgccctcgg
     1021 gtttgaggtg ctgcacggaa aaatccaccg ccagctccac caggtgggcg atgcgggcgg
     1081 catcggccga ttcaatgccc gacagattcg gcgccatgtc ggaaatcacg acgtcaacct
     1141 tggtaaagcc cttgtcggcg gccaaagtct gctccagaat ctgtagcacc tccggttccc
     1201 ggaagtcccc ctggatgaac accaccccct cgaccgggtc catcggcagg atgtccagcg
     1261 cgatgatgcg gccgttgagt tcgccggccg ccgcgcccga tggcgagagc ttgcggcgcg
     1321 catactggct ccaggcgccg ggcacgctgc ccaggtccac cacacagtag ccgggcttga
     1381 tgaggcccag catgtcatcg atttccttga gcttgtaggc cgcccgggcc cggtaaccct
     1441 ctcttttggc caacttgacg taggtatcgt tgacgtggtc attcagccac gctttgttga
     1501 cctttttgct ttgggttttt actttcatgc ctgtattgtc ctctcaccgc cttccggcgc
     1561 cgcggtttcc ccaacgataa atcagcggca gcggcggctt ctaggataat cagggcatgg
     1621 ctcaaatact acttaccccc gcccagcgca aagaccaccg cgccgatgcg caccacctgg
     1681 accccgtcgt gatggtcggc tcggacggcc tgacggccgc tgtcaaaagg gaaatcgacc
     1741 tggcactgaa ggcgcacggc ctgatcaagg tccgtgtgca gtcggacgac cgccctgcgc
     1801 gcgaagccat gttccaggct ctggccgacg aactcggggc cgcgcccatc cagcacatcg
     1861 gcaagctgct ggtgctgtgg cgcccgatgc ctgaaaagga aaaaaaggtc aacgaggacc
     1921 gcatgcccgg cccgcgcgac gtcaaggtgc tgaagaacag cgcgcgttac ggccagcgcc
     1981 ccgaagtgaa aaccctgcgt gtgctgggca accagcgcct gacgccgggc ggcaccgtga
     2041 aacgggcgaa accgaaaaag cagcaaagcg ccaagaaacg cgcccagggc tgagccggcg
     2101 gcggcgtgat ccgaccggtc cttggccggc cagcgaggca ttttcctttt ttgtctggcg
     2161 cttccccctt tagatcgggt gcctgcgccc acaactgatg gacatgacca ccttgaccac
     2221 attgaacgca tccaatccgc ttctcgattt ctccagcctt cccctgtttg accgcatcgc
     2281 gcccgagcat gtgggccctg cggtcgatgt gttgctggca caggccgaaa ctgcgctgga
     2341 acgtgtcacc acgccggagt ttccagcgcg ctgggcggac attgccggcg tgctggacgt
     2401 ggccaccgaa aacctgggcc gggcctgggg tgcggtcagc cacctcaaca gcgtggcgga
     2461 cagccctgag ctgcgcgccg cctacaacgc tgccctgccg cgcgtcaccg agttctggac
     2521 ccgactgggc gcagacgaac ggctttacgc caaatacaag gccatcgatg tggcgtcgct
     2581 gaaccccgaa cagcgccagg cccataaaaa tgccgtgcgc aacttcgtgc tgtcgggcgc
     2641 tgaactcgtg ggcgcagcca aggaacggtt tgccgccatc caggagcgcc aggccgaact
     2701 gagtcagaaa ttcagcgaaa acgcgctcga cgccaccgac gcttacgcct actacgccag
     2761 ggaagacgaa ctcgccggcg tgccggccga cgtggtccag acggcgcgcg cgcaggccga
     2821 agccgagggc aaggagggtt acaagctcac gctcaagatg ccctgctacc tgccggtcat
     2881 gcagtttgcc gacagttcct ccttgcgtga aaccctttac aaggcctaca gcacgcgcgc
     2941 cagcgaccag tcaccgccgg agttcagccg cttcgacaac agcgccgtga tgcaggaaat
     3001 cctcgcgctg cggcgcgagg aggcgcaact gctcggctac cgcaatttcg gcgaggtctc
     3061 ggtggtcccc aagatggccg attcgcccga gcaggtgatc agcttcctgc gcgacctcgg
     3121 ccagcgtgcc aagccctacg gcctcaagga cgtggccgac ctgcgcacct ttgccgcgga
     3181 aaagctgggc ttgaaagacc cgcagccctg ggacttcacc tggattggcg aaaaactcaa
     3241 ggaagcgcgt tatgccttca gcgaacagga agtcaaacag tacttcaccg cccccaaggt
     3301 gctggccggc ctgttcagga ttgtcgagac gctgttcgag gtggcgatcc atcccgacac
     3361 cgcgcctgtg tggaatcccg gggtcgagtt ctaccgcatc gagcgcgacg gtcagctggt
     3421 agggcagttc tatctggacc gtccggcgcg caccggcaaa cgcggcggcg cctggatgga
     3481 cgatgtgcgt gcccgctggc tgcgccccga caccggcacg ctgcagaccc cggtggccca
     3541 cctggtctgc aactttgccg acggcgtggg cggcaagccc gccctgctga cgcacgacga
     3601 cgtgaccacg ctgttccacg agttcggtca cggcctgcac cacatgttga cccaggtgaa
     3661 cgagcgcgat gtctccggca tcagcggcgt cgaatgggac gcggtcgagc tgcccagcca
     3721 gttcatggaa aacttctgct gggagtggga tgtgctcaaa cacatgaccg cccatgtgga
     3781 caccggtgag ccgctgccgc gcgccctgtt cgagcggatg ctg
//
LOCUS       DAIDFG010000297         3815 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_297_length_3815_cov_19.791489, whole genome shotgun sequence.
ACCESSION   DAIDFG010000297 DAIDFG010000000
VERSION     DAIDFG010000297.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_297_length_3815_cov_19.791489"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          693 a         1278 c         1194 g          650 t
ORIGIN      
        1 catcccatcc cgatccggct gccgcgcctc cgcaaggact tattcagcgc ttccttaggc
       61 caggcgcccg cctgatggcc gaccgcggcg attgagcatc aaatcggcct ccggtccata
      121 tcccacctgc gggacaagct atttatttga tagcaaacaa cgtgcaacac ggcagcagcc
      181 agcgcatcgg ctgacacaag aaccggccac ccgcctgcgc tgccgccacg gctgcgggcg
      241 gacaatgacc tctttgaaag gcctgcgcat gaacaccgcc ctgaaatacg ccctcgccgc
      301 cctgctgcct gccctgtccg gcggccaggc gctggcctgc tacaccgtct acaaccgcgc
      361 caaccaggtg atctaccatg ccgcgcaggc cccggttgac atgcgttacc aattgcacca
      421 gaccctgccc gcgatttttc ccggtggcca catggtgttt tccatcaccg acacctcgtg
      481 cccgccggtc aacctgacgc gcagcaatat cagggattcg gtgggcggca gcgtggctta
      541 cgcggcaggg cccggagagg ccatgatgtc acccaggaac cggccgtaca aagaggcccg
      601 gaactaacgt ccctcaagca aggtgaccgg gtgaacggtg cttactgcga gtgcgcatca
      661 aaaaacgcgt gcaccggcga gtcgtccggc agcacataca ccacgccccg atttcggccc
      721 agcgtcgggc ccaccacggc ttcataaaaa gccaccggca ccaccacaca gcccagcgaa
      781 atgcggttgt ccgccggact gccggaggcc agtcgctgcg cacgctgctc ctggctgctg
      841 ccgggacgca agcggtgaat cgccagacct tcccggtagt tgacccacac cacctcctcg
      901 ccctgcaggt tacgcccggg ctgcgacaca aaacgcccgg cgggggtggt acgttccgcc
      961 ggcaggatgc gcgacagctc gcgctcgccg atgccgggca ccgcgtggtc gccgctggcc
     1021 agacccagca acacggccga ggcgccgcgc aggcgaccgc ccggcccgaa cacaaacatc
     1081 ctcgcgtttt tcttgtcgac aatgacaaag ggcagcccct ggtggtcgga cgcggccagc
     1141 acccggcttg cggcgtagcg ggcttcggca gacgctgcct cccccgcaaa gtctgccgcc
     1201 agcacgggct gagctcgggt gcccgctttc gccaaggggg cggcaaacag ggccaggact
     1261 cccagcgcca gcgcggcgcg ccgcatgcgg ctcttgatgc tcatccagtt ccccggtgtg
     1321 tttgtgacga cacatcaccg ggcttcttcc cgaagatgta ggaaacatcc tacacggcaa
     1381 ggccgcaaac tggcaagccc gaatcaggtc cggatctgaa aagattattt tgatagcagt
     1441 aggccctttg ttaaaaaggg ctggggtggg atttaatctg cagtgggcag atcagcggcc
     1501 gggcgggatg taggcgccgt ctgacagcac gaccccggtt caggacggct ggcgcgtggc
     1561 aaccaggtcg ccaaacacgg actgcagcgg ccgcgtttca ggcaggacat aaaccacgcc
     1621 gcgtcgcgtg ccgagcgcgg gcttgaccac cgtgtcataa aacccggccg gcagattgat
     1681 gcagccaaag gaaatgcggt tgtcgtccgg tgttgcggat gcgagccgct ccagccggcg
     1741 ctctttcggg tctttcgccc gcacgcgatg catggacact gccgccgcgt agtccaccca
     1801 gacaatgtct tcgccaaagg agttcacgcc gggcagggaa acgaaccggc ctgcaggcgt
     1861 ggtgcgttcg tgcggcagga tggccgacat ttcacgctca ccaatgccgg acaccgaatc
     1921 gtcgccgacg gcagaaccca tcagggccgg ggtggcgccg ctgagttgtc cgttcggttg
     1981 aaaaacaaac accctgacat gctgcttgtc gacgatgaca aatggcatgc cacggtgatc
     2041 gcgcgatgcc atcacccagt cggccatctg gcgcgcttgc agcgaaggct cgtgggacag
     2101 aaaatctgcc acccggggga ggccgtccac ggcggacacg acggcacttt gccggctctg
     2161 tgtcgacgtg acatgtcccg gcagcaccag agccagcacg gcacaaccca tgcccagccc
     2221 cttgaggatg gcgtcgaagg gtgaagcgtc tgccggggtc atgtcagaca tatcgggatg
     2281 tcaggttgca cgcgcaaacg gaagcaggga cgcgccatga caacatggga cgtccctgcg
     2341 aaagccttgc gcatgaggga gacgcaggct tagttgcgat cagcgcgggg ctcgggcatg
     2401 acggtgccgc tttgcatcgt ggcgctgttg tccaccgaag taccggtcgt gctgctggta
     2461 gcgggctgag cctgcatggt cgtgccggcg gcggggttga cgtcaggtgc cttcatctct
     2521 gcggcacgct ggctgttcag gtcaccgggg gcaccgggtg ttgtgccgga ttgggcatag
     2581 gcaaaaccag cgccggcaac gagtgcggca gcagtgacgg cagcggtcaa ggtttgattg
     2641 aatttcatga gtagctcctg aatgaaaaac cccggtaagg ggcgatgggg tgtatgccga
     2701 ccggagggcc gactggttcc aatttaggac ggccctcatg ccgctggagt gggcctcacc
     2761 cgcgtgcgcc tgtaggactg gtccggtccg cagcccggcg ctgcatattc aatagcgtat
     2821 cgaatggacc tgtcatcgcg ggcgggcctc tctgcctcac ggcgccccgg cgcccatcgc
     2881 catttctatt cagggcttcc ggtgccacgc cggacacggc ctacagcgtt catcagcgcc
     2941 ggcctgcaac acggaccggc cgtgcacaac gacactggca ccgtgcccgc cgggttcgcg
     3001 ctacaaagac aggtatcgga cttccgccct ttggcgaaac gtcggaacat tgcccagcga
     3061 acccggcggc atctttggca tccggctttt gcgtcttacg ccggcagtct gccggccatg
     3121 gccttgccga cctcgttgct gccttgcgca tcgccgcttt gcggcaccat ctcgccggcg
     3181 cgttccgtca cctcggccag ccgcgcggcc agcgcctccg gcacctgctc tcccgtgccc
     3241 aggtacacgc cctgggtcag cgtgatgtgg gccgcctcga aagtgccggc gcccgcgcag
     3301 agaatggtgc gtgtcggcgc atcctgcgcc gccagcacca gcatggccgg caccacggct
     3361 tcgggctgca gcgcctgcag cacctcttcg ggcatcagcc cttccgtcat gcgcgtggca
     3421 gccgtcggcg ccaggcagtt gacgcggatg tcgtttttcg cgccttcgat ggccaaggtc
     3481 tgcatcagcc cgaccagagc catcttggcc gcaccgtagt tggactggcc gaagttgccg
     3541 tacaggcccg atgaggatgt ggtcatcacg atgcggccgt atttctgcgc cgccatgatg
     3601 tcccacacgg ccttgctgca atgcacggcg cccatcaggt gcacgttcat cacaagcgcg
     3661 aagtcggcca ggtccatctt ggcaaaactc ttgtcgcgca ggatgccggc gttgttgacg
     3721 aggatgtcca cgcggcccca ggtatccatg gcctgctgga ccatggcctg caccgccgca
     3781 aagtcggtca ccgaggcgcc gttggcgatc gcttc
//
LOCUS       DAIDFG010000298         3815 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_298_length_3815_cov_17.066755, whole genome shotgun sequence.
ACCESSION   DAIDFG010000298 DAIDFG010000000
VERSION     DAIDFG010000298.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_298_length_3815_cov_17.066755"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          599 a         1169 c         1364 g          683 t
ORIGIN      
        1 gactcggggt agggcgacag gttggccagc tcggccacca ggccagcgcg ctggcgccgg
       61 gtttcgccca tgccgacgat gccgccgcag caaaccttga cgccggcatc gcgcacacgt
      121 gccagcgtgt cgagccggtc ctggtagtcg cgcgtggtga tgatgtcacc gtaaaaatca
      181 ggcgcgctgt cgaggttgtg gttgtagtag tcgaggccgg cgttttgcag tgtttgcgca
      241 tggccgtctt ccagcatgcc cagcgtcgcg caggtttcca gcccgagggc cttgaccgtg
      301 cgcaccagct cggccacttt ttcaatgtcg cggtctttgg gcgcgcgcca ggccgcaccc
      361 atgcaaaagc gcgtggcacc ggcatccttg gcgcgttgcg ccgccagctt tacctcatgc
      421 ggctccatca gtttggtggc ctccacgccg gtgtcgaagc gtgccgcctg cgggcagtag
      481 ttgcagtctt ccgggcagcc acctgtcttg accgacagca gtgtggcaaa ttcaatgcgc
      541 gttggatcaa agttttcacg gtgcacggtt tgtgcctggt gcatcagctc gggaaaaggc
      601 aggtcgaaca gtgcttcgat ggcatccacg ctccagcggg cctcggagct ggccgggacg
      661 ctctggcgcg ggcgatgcag ggtgacgggt tgttcaaaaa cgctggacat gtcgtggctc
      721 ctggaggtga gagtcgtttg accccgggtt tgatcgggag ggtggtgcgg ccggatcaga
      781 tgccttgtac tgcaatggtg gagcgctctt cccggatgcg gttggcgctg tcgacaaaca
      841 ccagtttagg ctggaagctg gccacctggt ctgccggcaa ctggccaaaa gctgcaatga
      901 tgatcaggtc accgacggcg gcctggcgcg ctgccgaacc gttgacggaa atagtgccgc
      961 tgccgcgctg ggcgcgcatc gcgtaggtca caaaacgcgc gccgttgttg atgttccaga
     1021 tgtggatctg ttcgaactcg gcaatgcccg aggcttccag caagtcgtcg tcgatggcac
     1081 aggaaccctc gtaatgcagt tcgcactggg tgacggtggc gcggtggatt tttccggcga
     1141 gaagggtacg ttgcatggag tctcaagccc ttaaaccgtg ggtgaaggcg tgagtgaaag
     1201 tgtgggtttg ggaaacaggg gcgccgtgac ggggggactc gcccggccac gcagcacggc
     1261 ctcctgcaac tgtgcgatgg ccgtttccgg tgaggccgct gcggtgatgg cactgatcac
     1321 cgccacaccg gcggcgccgg cccgcgcggc ctgttcggtc cgcgccacgt ccatgccggc
     1381 aatgccgacg accggcaggg gcagcagggc gcaccagtag gccaggttgt tgttgccctg
     1441 cggtatccag ggcatggcct tggcctgggt ggggtgaatc ggcccgcagg cgatgtagct
     1501 gggccgcagt gcccaggcgc ggcagacttc ccagtaagca tgggtgctga tgcccagccg
     1561 caagccggct tgtttgatcg cagccaggtc ggccacgtgc aggtcttcct gccccaggtg
     1621 cacgccgtac gcgccttcct cgatggcgag ctgccagtgg tcgttgataa aaagctgggc
     1681 gccggctgcg gtggctgcgc gaatgctttg gcgaatttcc ttgcgcacca tcgcgcggtg
     1741 ttgctcacct tccgcgtcct tgatgcgcag ctgaaccgtg cgcacgccgg cagcaacgac
     1801 gcgggttacc caggcggcgc tgtcgaccac ggcatacagt cccaggtccg ggtcgctgag
     1861 tgcggcaaac ccgctgacgg tcgtgggccg gggcaggctg aagtgcggca ggttgctgct
     1921 aagccctgca aaatcaggac gcggctgcaa caggcctgtc agggcctgtt gtcgcagtgc
     1981 atctgctgtc gccatgcgtg ccaggatgat ggcttccatc gcgacgaaac cctgtgccag
     2041 ggccgcggcc gcgctggcgg tgaacacggc gtcggcaccg gggacggtga gcggcggcgc
     2101 ggcaccgtcc ggcagctcaa gccagccgct ggcctgcggc gtgtcggcat agtcggcttc
     2161 gtcgccggtg atcacgaccg cctcgctaac ggcggcgcgc aaacgggctg cggcctgtgt
     2221 cacggcctcg gggttgttca gtgcagccgt gccgagcaag gtggcggctt ccctgcgatc
     2281 caggcagagc agggcgggac gcgggaggtc ccgcgcgctg ccggccagca gcgcttgtat
     2341 gtccttcgcg ttgccggcaa aggtccagac cgacggggtg ttggcggcgc tcacaggatg
     2401 aagggccggc ctgtcaccgg tgtggaagcg accgccatgt cctgctgggc catgatgccg
     2461 gccttgtagg ccaggcgccc ggactcgatg ccgagcttga aggcgcgtgc catctgcacc
     2521 gggtcgcgcg catgggccac ggccgagttg agcagcacgg cgtcgtagcc gagttccatg
     2581 gcctgcaccg cgtcggctgg cgagccgatg ccggcatcca cgatcagcgg cacaccgggc
     2641 aggcgggcgc gcaacgtgcg cagcgcgtag gggttgacca gaccctggcc cgagccaatg
     2701 ggtgcgcccc agggcatcag gatacggcag ccggcgtcga gcaggcgctg gcagctgacg
     2761 aggtcgtcgg tgcagtaggg gaacacttca aaaccatcgc gcaccagttc agttgccgcc
     2821 gtcaccagtt caaacgggtc gggctgcagc gtgtgttcgt cgccgaccac ttcgagcttg
     2881 atccagtggg tgccgaacag ctcgcgtgcc atctgcgaca gggtgatggc ttcgcgtgcg
     2941 ctgcggcagc cggcggtgtt gggcagcagg cgggcaccac tggcccggat catcttataa
     3001 aaatcgtttt gtgtgcccgc gccgccggcc gccatctggc gcttgaggcc gacggtcacg
     3061 acctcggcgc cggaggcggc aatcgcgtcc tgcaggacct gcggtgaggg gtagctggcg
     3121 gtgccgagga aaaagcggct gttcagttct gtatctgcaa tgcgaaatgt catggcgtgg
     3181 ctagcctccc gtgatggctt gaaaggtaaa aacggcgtcg ccgtctgcga gtacggtgcg
     3241 ggcgcgcgcg tcgcgcgcca caaactcgcc gttgacggcg ctggcgaacg atgccgcggg
     3301 ctggccgccg gccgccaggg catcggccag cgtgctgcct gcgggcagct cacgggtttc
     3361 gccgttcagg gtgatggtga tggatgtcat gccaggtcct tgagcgtttg ttcgaccagc
     3421 gccggcgcaa tcagccagcc gtggcggaac aggccgttga tgcggaccag cccggggctt
     3481 tggtcgacgc gtggcagatt gtcgggcagg gcagggcgca aattcgtttc ggtatgaaca
     3541 atacgcgctt cagccagttc cggaatgatg ctgtgcgccg cagccagcag ttcgaccgtg
     3601 ctgcgcagcg agatcggcga gcggtcctcg ctttcgattt cgctggcgcc gacgacgatg
     3661 tggccgcctt cacgcggcac caggtagacc cggtgccgcg ggtgcagcag gcgcaccggc
     3721 cgctgcagtg ccacgccggg cgcatgcagc cagatgattt ctccgcgcac gccgcggacc
     3781 tgcggcgtga agccgtcgtt gggtgcaacg ccggc
//
LOCUS       DAIDFG010000299         3795 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_299_length_3795_cov_17.089840, whole genome shotgun sequence.
ACCESSION   DAIDFG010000299 DAIDFG010000000
VERSION     DAIDFG010000299.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_299_length_3795_cov_17.089840"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          680 a         1216 c         1136 g          763 t
ORIGIN      
        1 accggtttca tggaggccgg cgaaacaccc gaaggcggca tcacccgcga gatcaaggag
       61 gaaaccagcc tcgacgtgac cgacctcaaa ctgatcggcg tctacgactt ccagcgcatg
      121 aaccagatca tcattgccta cagcgccgtc ggccacggtg aagtgaaact ctccccggag
      181 ctggtggact accggctgta tgcccacgaa gacgtcaagt gctggccggc cggcaccggt
      241 tacgccctgg ccgactttct gaagtccagg ggttacgcgc cggagtttgt ggagtgggcg
      301 aagcgggatt gagggcctgg cggcgcggga cttggtgctt gtgagtattt ttgggctgca
      361 gcccttgttg cacgggcgca gacagctatg aaaagcgtag caatggctga cgggcacgtc
      421 gatcctgcca ggcaggcgcg tcgtgtgcag cggtcccccg gcctgaatct ctgtcatttc
      481 agacttttta ttttctggcc ccgcgtcaca aactgaaaag ctcaatcgtc aatacggtgt
      541 cacctccagt ttctaaagga aaacaccatg actgctaccg ccactgccaa taccgccccc
      601 gtccgtttgg acctcgtcgt tcccgacgtc atccccgccc tgatcaaggt gcgtgaagcc
      661 gttggtggcc acggactcga cataaaactt caccacctgg tgcacctgcg cgcctcgcag
      721 atcaacggct gcgccttctg cgtcaagatg cacacccgcg aagcccggca ggacggcgaa
      781 accagcgaca ggctggaccg cctcgttgtc tgggcgcatg tgagcgactt cagcgaagcc
      841 gagaaagccg cactggcctg gacggaagcc ctgaccgtgc tcaaccccca gaccgactac
      901 gccagcctgc gcggccggct acgtgcctct ttcaccgaca agcagatcgg cgcactgacc
      961 gctaccgtcg gcatgatcaa cctctggaat cgcctccagg tctcggcaca ctgagtaaac
     1021 ccgagacgcg cagcatgagc accattgacc ccaccgccgt ctttgaagaa aggcgcaggc
     1081 atttgttcgg cctggcctac cgcatcctcg gttcacgggc cgatgccgaa gacgccgtgc
     1141 aggatgtctt catcaaatgg caggcggccg accgcgccgg cattgccacg ccggcggcct
     1201 ggctcaccac gctctgcacg cggcactgca tcgacatgct gcgcgacgcc cagcgcagcc
     1261 gggtcgacta cgtcggcacc tggctgccag aaccgattca gacccgcacc gaagacacgc
     1321 ccgaaacgca ggccatgctg gcctcgtcgc tgagcatggc tttcttgctg ctgctggagc
     1381 ggctcacgcc cagggagcgc gccgcttacc tgctgcacga gatttttgaa acgccgtacg
     1441 ccgaagtggc ggagatgctg gacatggaag aagctgcctg ccgcaagctg gtctcgcgcg
     1501 ccagcatcag cattgcccag ggcaaggtgc ggcacgtgac gccgccccag gtgcaggacg
     1561 gcctgctcgc cgcattccgc gaagccatta ccgccggcag caccgggtcg ctcgccagcc
     1621 tgctctcgga cgacatcgcc ctgtgcaccg acagcggcgg caaggtcgcc tcggtggaag
     1681 gcatcttcca gggcaagggc agcgtgctca ccctgctgac gacgcaactg gcccgctact
     1741 gggcccacta cgaatggctg ccctgcgaga tcaacggcgg ccgcgccatc cagctgcgca
     1801 agaacggcga aatcgcggcg gccatcacct tcgcctacaa cgacaaaggc caggccacca
     1861 acatctacat catgcgcaac cccgacaagc tcgacgggct ggccttcggg ccggaggttt
     1921 tgtcgtgagg ctggtgcgct gcggtgtgaa atgagcccga aacgtcattg atataagcgt
     1981 tttggctctc cagcccttat ggggtgggcg caagcagcta tcaaaaaaga agcgccttgc
     2041 aagctgtccg atggacagcc ggccggcctg tcgaccgtac cgccccggaa ttaccgcagc
     2101 tgacgaagcc gggggcctgc atcggggggc gccgcgtcgc ccgccttggt aaactatgtg
     2161 tgtgtgcctt tgtagctcag tggatagagc gatcccctcc taagggatag gtcgcaggtc
     2221 cgattcctgc caagggcacc attttctttt tacgctgccg tgccaatcac gcgctgaatg
     2281 gcgatgccgc ttcaggccgt gcgttacttc gacagcaggc gcatcgcctc ttccagcccc
     2341 ttgatggtca aggggtacat gcgctggttt accagctgct ggatcacgct gatgctctgg
     2401 cggtactgcc agacgccttg cggctcgggg ttgatccaga caaatttcgg gaaggcgttg
     2461 gtcagccgct gcatccactc ggcgccggct tcctcgttgt tgtattcgac gctgcccccc
     2521 ggttgcaaaa tttcatacgg gctcatggtc gcgtcgccca caaagatcag cttgtagtcc
     2581 ttgttgtact tgcggatgat gtcccaggtc gcaaacttct cgctgaagcg gcggcggttg
     2641 ttcttccaca tgaagtcata gacgcagttg tggaagtaat agaactcgat gtgcttgaac
     2701 tcggtcttgg tggcggagaa cagttcttcc acgcggctga tgtgttcgtc catggtgccg
     2761 cccacgtcca tcagcagcag caccttcacg ttgttgtggc gctccggcac catcttgatg
     2821 tccagccagc cggcgttggc cgcggtggac ttgatggtgt cgtccaggtc cagttcctcg
     2881 acatggcctt cacgcgcaaa cttgcgcagg cggcgcagcg cgaccttgat gttgcgcgtg
     2941 cccagctcct gggtgtcgtc gtagtccttg taggcgcgct ggtcccagac tttcaccgcg
     3001 cttttgttgc ggcttttttc ctggccgatg cgtatgccct gcgggttgaa gccgttggcg
     3061 ccaaacggcg aggtgccgcc cgtgcctatc cacttgctgc cgccttcgtg gcgttccttc
     3121 tgttcctcga aacgcttttt cagcgtctcc atgagctcgt cccagcccat cttctcgatc
     3181 ttggccttgt cttcggggct gagttcgagc tcgaggttct tgcgcagcca ctccagcgga
     3241 atgtccttgg tgaagtcggc aatcatctcc acacccttga agtaggccgc aaaggcgcgg
     3301 tcgaacttgt cgtagtgttt ttcgtccttc accagcacgg tgcggctgag gtaatagaag
     3361 tcgtcgatct tgtaggcgcc ctcgccgatc tcgccgtcct cccccttgcc gctgttgggc
     3421 ccgaccacgc cggcctgcag cgcttcgagc agcatcaggt attccttgac cgagaccggc
     3481 agcttggccg aacgcagggt gtagaagaag tcgatcaaca tggcaggact cctttcaggc
     3541 ttttggcgcg cgtggtttgt cgagcaggta caggagttgc gctttcactt ccggccactc
     3601 gccggagcgc aggctgtaca tcaccgtgtc gcgaatcgtg ccgtcgcgcc gcagcgcgtg
     3661 gccgcggatc acgccgtctt ttttggcgcc caggcgctcg atggcgcgct gcgaggcgaa
     3721 gttgaagttg tcggtacgcc agcccaccac gtggcagccc agggcctcga acgcgtgggt
     3781 gagcatcagc agctt
//
LOCUS       DAIDFG010000300         3763 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_300_length_3763_cov_17.679612, whole genome shotgun sequence.
ACCESSION   DAIDFG010000300 DAIDFG010000000
VERSION     DAIDFG010000300.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_300_length_3763_cov_17.679612"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          665 a         1207 c         1182 g          709 t
ORIGIN      
        1 gcaaccatga cggccacttg actccgacat gacgcgctac gcgcgtcatg tctccccgca
       61 cccgaacccg tatttgtttc ccccacccgt cgggctgggc cgaggagcgc aggcaaaagc
      121 ggatcagggc tcgcgattgt ttgagacggg agagtgctag cgagaccccg cttttaccga
      181 gcaccgcagg ttgcccgcag cgaagcgaag ggatccagac catcgggtcg cttttttctt
      241 tgcttacttt ctttttggcg acgcaaaaag aaagtgagtt gccgccgggc aacccccggc
      301 ttgctggaag acccccaacc gcgcaagctt gcgcaggctt cgacacgctc agcccgaacg
      361 gagtcagagg aacgcccgca tggatcccgg ctcaagtccg ggatgacaag ccacagaaaa
      421 catttgctat aaattcaata gctgtctacg ccctatcaat aagggctgcg accctgaatc
      481 gcttttaaaa atacgtttgc cgacaccaca ggcggcaagc gcctactgca catgacattc
      541 ccgccgcagc agcgccacag cgtcattgag cgcgtgaacc gcatcgcttt gcagcgtggg
      601 ctgcgcctcc tgcacaaact cttcccacaa gctggtgtag gcctcctggt gttgcgccgg
      661 ggcgtgatcc acgatgtact ggtgggccag tgcgggatgg aaacctgatt tccagatctt
      721 gcgcaccagg gtcgcggcgc ctttttcggt cagcatcgtt ttgggcgtgg cgccggcggc
      781 gacgcacagc aacaaggtca gcagagaacc atcatcgctg ttgccgccgg tgaccttgtt
      841 ccaggccgcc gacgcctggc ggtcgtggtg gtcgacttcg gccaaagcgt cttcgaccca
      901 gaaataccgg cccatgaacg ctgggctgcg gtcaaacagc tggcgcaccg gcagggcgcg
      961 gtcgaggatt ttcggcacgt cggtcatcac ggactggcgc acgagctcca ccacgccatg
     1021 aagatgggca ggcagttttt tgggcaaggc gacaggcaac gccgccgggg cagccccatg
     1081 ttttttgcgc agggcaacga tcatcttctc gaacgcgatc cagtcgggca tctcgatgcg
     1141 gcccactgcc agcgccagca aggcggtgcg gatcacggcc tcggcgtcct tgccggcctc
     1201 gtcaagctcg tcggcgtcca tgcccagttc gccggccagc cacaaggccg cgtccaccac
     1261 ctgcgccacc tggctgcgcc gggcgagttc ggcctggtag tcggccaggc tgcgcagcga
     1321 ccatttggcc agcagcggaa tgtgttcgtc gcggaagccg ccgcgttcgt tcatgccgaa
     1381 gtggctgttc tgcggcatgg cgatcagcgc cttgagcatg tcagagccgc ccttggagcg
     1441 cgacagcagg gaaaaatcac gcagcgactc ggccgcctgc ttcaagtcgc cgccgcagct
     1501 gctttccagg taaatgctga tcaggttgac catgcggtcg cgggcctttt caagctccgg
     1561 gcgcaaaaac tcgttgccga aataacgcgc gatctgcacc atgcccttgg ggccctcgat
     1621 acgcatggcc tcgatttttt ccggaccaag gatgccgtgt tgcacgccat gctgcagcgc
     1681 cttttcgaaa aaaggtcggg catcgtacag cgagacggcg gaaggcgtgg acacggtgtt
     1741 tgcttcggtc aggagatgat ggggttcaga tcgccgattc ctcgtcggac tcgcgcgccg
     1801 tcggtgtggc cttgccacgg tcggtgtcgc cggtctcgac cttgccgtcg tcctcggcgt
     1861 cttcctcgtc ttcatcctcg gaagcggccg cctggtgagc actgaccttg gcgcgcagca
     1921 ccatcagcat ctcgatgaac aggtccggca gttcgtaacg cagaatccag gcgagttcca
     1981 tccagtcatg gtcggcgatt tcatcctgct ggacacgcgg tttctcgtag gccgaggcca
     2041 gcagggccgc ctgggacagc atcttgccgt actcgtccgc cgcctcgaag ctgccgctgc
     2101 gggcaaaacc ctcgacccgg ctccattggt cggaaaaata gtcggcgagg gcctcgctgc
     2161 cgccgtccag gtcgccaatc gcattgagaa agtccagcgg gtcggcgtcg tcccggaaga
     2221 tgactgcgtt gaccatgccg cgcagatggg tgcgggtcag gtccttgtag agcttttcga
     2281 tcaccacggc gcgggcgaac tgcgcgggcg acaccagcag gctgaccacc gactccttcg
     2341 aatggtcgta ttcgcggatg acggccagga tgtccttggg cggcagttgc tccagcacca
     2401 ccatcagcgc ggcatcccct tcggtgtcgg ccagcgtgac cagggccgac tcggcgccca
     2461 cgatgtcgcc ggccgcaatc aggctttggg ttttttctat cagggcgagg tgctgggtca
     2521 tcagggtttc actttccggg tcatgtggct ggcatcagtc tttctggtcg aagccctgct
     2581 ggaccagcca gtcggcaccg gcttcctggc gtgcgcgggc atcactaccc tgcccgccct
     2641 cgccatcgtc ggcaaaaatc accagctcgt catcgccgcc ctggtcgctg tcttcgtcat
     2701 cttcttcgtc gccgcccagg gcgagccccg ccgcggctac cggcaggccg gccatgtatt
     2761 ccagacccgc cgtcagtgtc ataaaccggt cgccgcccgg cgactccaga acgatctgcg
     2821 ccagatcctg tgcccagggc gccagctcac agctgcccgc caggctgctc caggccggaa
     2881 aaacatcggc gtcatgggtg agcaggtgat ccatgacgac ccgcccggtg aagtcaaacc
     2941 cctggtgaaa caccaccgcc accatctcgg ggctgagttc catcatctgg agcagaaagg
     3001 cgagctcctg gcgtttgccg gccagccgct ggtgcagcac gtcgcgcccc tcggagtcga
     3061 agcgcaggtc atccatctcg gactggtagg ccgaacgcag gtcgaggaaa tagggggaga
     3121 tgctcatgcc accgggtttt tcattgttca gttccatggg tcagcggagc tcagaggtga
     3181 gcagcgggtt gaaatagttt tgccagatat cggtcgcggt ggccagcggg ctgtcgagca
     3241 ggttgcggcg ccggttgtcg tcgtaggcct ggcgtttgtc cgcgtccgac agcacttcgt
     3301 acgcttcctg caccgaccga aaacgcgccg gcgcctgcgg atcgggattt ttgtcggggt
     3361 gccagaacga cgccttctgg cgaaaggctt tcttgacgtc ggccagcgtg gcagcgctgt
     3421 ccagcccgag ggcggtgtag tgatcggtca tgcgcaggtg gcgttgtcct gggccgcggg
     3481 caaggcactg ccacggcatg ttgaagggct caccggcagg aaaaagcgca ggcgcgggcg
     3541 acgaggggac ccaagattat gcacgtccgg ccctttccag ggcgcgaggc cggttaatgc
     3601 ccttgcgccg gactggccgc ccatttcgac aggcgccccg acaccagcct gaaacgccgg
     3661 ccacaacgcg cggccagtgt ttcgtcgtgc gtcaccatca caaaggcggt gccgtgctcg
     3721 cgggcgagtt gcagcatcag ctcgaacacg ccgtcggcgg tgg
//
LOCUS       DAIDFG010000301         3758 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_301_length_3758_cov_16.243586, whole genome shotgun sequence.
ACCESSION   DAIDFG010000301 DAIDFG010000000
VERSION     DAIDFG010000301.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_301_length_3758_cov_16.243586"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          621 a         1133 c         1262 g          742 t
ORIGIN      
        1 cggcggtttc gcccaggtag ttggggtcga tggcgtcgag cgcaaactcc tgctgcaggc
       61 gctggatgac ttcggccgag ggcatcacct cgtccatgcg ccagccgttg gtggcgccca
      121 catccatgta ttcgatgaag cgcagcacca ccccggagtt gcggaagtgc cgggccatgg
      181 gcaggatctc ggcgtcattg gtgccgcgct tgaccaccat gttgaccttg atgggcgcca
      241 ggcccacctt gtgcgccacc tcgatgccct tgagcacgtc ggccaccgga aaatccacat
      301 cgttcatgcg ccggaacacc gcgtcgtcca gcccatccag gctcacggtg acgcgctgca
      361 ggccggcgtc tttcagggcc tgggcctttt tgaccagcag cgaaccgttg gtcgtcaggg
      421 tgatatccag cggtttgccc tgcggtgttt cgagccgggc cagcatcccg atcagcactt
      481 ccaggtgttt gcgcagcagc ggctcgccgc cggtcaaccg gattttttcg acaccgtgcg
      541 cgacgaaaat tttcgtcagg cgcgtgatct cttcaaaact gagcagcgag gtctgcggca
      601 aaaaagcgta gtccttgttg aagacttcgc tgggcatgca gtaactgcag cggaagttgc
      661 agcggtcggt cacgctgatg cgcaggtcgc gcagcggccg cgccaacctg tcggccagca
      721 ggccggtggg cgcctgcagc tgggcaggga tgtgcggcac gcgcgcggcg tagcggatgt
      781 ctgcgagggg aatgattttt ttctcggtca tgtgcacctt cttacccgca atttaacgca
      841 ttggggccgt gccggccgtt tggctgccgc ttgcccctgc tgaccgcgct taaacgcagc
      901 gcccgccgtc cacctcgata cacacgccgc tgatgaatgc cgcttcgtcg ctggccaggt
      961 agagcgcggc gttggccacg tcgagcgccg ttgaaaaacg cccgagcgga atgctggaca
     1021 aaaatttggc gcggcgcgct tcgccttcgg ggccggtaac cgtgccgccg gcaaactggg
     1081 tggacagcgc ggtgtcgggg ttgaacaccg ggttgatgca gttgacgcgg atgttgtccg
     1141 ggccgagttc ggccgccagc gacttcgatg tggtgatgac ggcacccttg gagccgttgt
     1201 accaggtcag gccggggcgc ggacgcacgc cggcggtgga tgcgatgttg atgaagctgc
     1261 cgccgccctg ggcgcggaac accggcgtgg cgtggatggt cgagaggtaa atgcttttca
     1321 cgttgatcgc gtagcagcgg tcgaattctt cttcggtggt ttccagcgcg ggctgattgc
     1381 ggtgcgtcca gccggcgttg ttgaccatca cgtcgagctt gccgtaacgc tgcacggccg
     1441 cagccaccag cgccttgacc tcggcggttt tggtcacgtc ggccgcaaag aaggacgcgg
     1501 tgcctccggc cttcacgatg tcggccacga ctttttcacc cagggcggtg ttgatgtcgt
     1561 tgacgatgac tttcgcgcct tcagcggcca ggcgtttggc aatgccttcg ccgatgccgc
     1621 cgcccgaacc ggtgacgatg atggatttgt cctggagtcg catggtgttt ccttgggtgc
     1681 tattaattca gtagctgctg gcgccctatg ggaaagggct gcagcctgtt ttttcttgag
     1741 aaaagggggg cgggttcagc cgtgccggat ggcgacggtt ttcagtgtgg tgaagccgta
     1801 cagcgcctca aagccttttt cgcgcccata acccgatgac ttgacgccgc caaagggcag
     1861 ctccacacca ccgccggcgc cgtagttgtt gatgaagacc tggccgctct gcacacgttt
     1921 ggccatgcgg aactggcggc cgccgtcgcg ggtccagatg ccggctacca gaccgaactg
     1981 ggtggcattg gcgagttcga ccgcgtggtc ctcgtcgctg aaactcatgg cgcacagcac
     2041 cgggccgaag acctcttcct gagccaggcg gtgtttcacc ggcacatcgc gcagcaaggt
     2101 gggggcctgg taaaaaccag tgtccggcgc ttcgtcgacg atctggccct gcgcgaccat
     2161 ggggatgcct gccacctgcg cgtcgctcag aaagtcccag acgcgttgct gctgggtctg
     2221 gcggatcagc ggtcccacgt ccagatccat cgtggcggga ccgacgcgca gcttctcgaa
     2281 agccagaccc aggcgctcca gcagcggttc ataaatgtgc tggtcaatca gcaggcgtga
     2341 gccggccgaa caggtctggc cggcgttttg cacaatcgcg ttgacgagtg ccggaatcgc
     2401 catgtccaga tcggcgtcgg caaaaacgat ttgcgggctt ttgccgccga gttccagcgt
     2461 caccgggcaa tggcgctcgg ccgccacctg ctggatcagg gtgcccaccg tcgggctgcc
     2521 ggtgaagctg atgtggtgga tgcccttgtg gcgggccagg gcgtcaccga cctcatggcc
     2581 gtaacccgtc acgatgttga tcgcaccggc cggaaaaccg acctcggcgg ccagctgggc
     2641 cacgcggatc agcgacaggc aggcgtcttc ggccggcttg accacgcaga cgttgccggc
     2701 ggccagggcg ccgccgacgc tgcggccgaa aatctgcatc gggtagttcc agggaatgat
     2761 gtggccggtc acgccatgcg gctcgcgcca ggtcatcacg ctgtagccgt ccagataggg
     2821 cagggtctcg ccgtgcagct tgtcgcaggc gccggcgtag aactcgaaat aacgcaccag
     2881 cgcggcggca tcggccttgg cctgcttggt gggtttgccg cagtcgcgct gctcgatcag
     2941 ggccagttcg tcggcatgtt cggccacctt gcgtgacagg gccatcagca gccggccacg
     3001 ttcggctgct gtgaccttgt gccagacggt gtgcaggcag tcgtgggcgg cctgaaccgc
     3061 cgtgtcaatg tcctgcgcgt tgctgcgctg gatctcttcg aacacctgtc cgtcggacgg
     3121 atcgatcaca ggaatggttt tgcccgaggc ggagcggacc gacgcgttgg cgatgtaatt
     3181 gagttgcatg catatgattt tccgcgaaaa cgcagggttc gcaaaaaatc aggccggatc
     3241 gggccgcagc ccccttttca ggaggggaat acgctatgaa atgaatagct gttgagctac
     3301 ggctgcgcgg gcttatgccc cgaccggggt gtaggcgctg gtgttgctcg ccagccagcg
     3361 cgccgacagg tcactggcct gctggttggg gtgaaagtcg gtgcgcagat agtcgcgcca
     3421 gggtttgaag gtctggcgca ccagaccgcg ctggccgaac aggaagccgg cggcgctttt
     3481 ccaggtgcgc cactgccaca aggtgccgtc gtgctgcagg ttgttgacgg tctggcgcaa
     3541 ggtgtcggtc aggaacacga aggtgatgcg gcggaaccac ttgatgcgcc actcgtggct
     3601 gccgcccagt gcggtgtaca cgtcgaaggc cgtgttcttg tgctccgact cttcggcgca
     3661 atgccacagc cacatggttt tcaggcgcgg ctcgcagccg tcgagcaggt cgtggttgct
     3721 cagcatccat tcggccagaa tcgcggtgaa gtgttcgt
//
LOCUS       DAIDFG010000302         3757 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_302_length_3757_cov_16.103187, whole genome shotgun sequence.
ACCESSION   DAIDFG010000302 DAIDFG010000000
VERSION     DAIDFG010000302.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_302_length_3757_cov_16.103187"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          608 a         1312 c         1267 g          570 t
ORIGIN      
        1 cggggtcctg ctgcaggccg gcttcggcga gttttttctc cagcgcttcg ctgccgtcaa
       61 accagaaatg ctctttggcc agttgctcga ccagttcggg cgccacgccc agcgccttgc
      121 ccacgtcgcg gatggcgctg cgcggccggt agctgatcac ggtggcggca atcgccgcgc
      181 ggtcgtggcc gtatttggcg tagatgtact ggatgacttc ctcgcgccgc tggtgctcga
      241 agtccacatc gatgtcgggc ggctcgcgcc gttcgcggct gatgaaacgc tcgaacagga
      301 gctggctttc cgccggattc acggccgtca cgccgaggca gaaacagacg gccgagttgg
      361 cggccgaacc gcgcccctgg cacaggatgc cctgctgccg ggcaaaacgc acgatgtcat
      421 gcacggtgat gaagtacatc tcgtagccga gttcctcgat caggaccagc tcgtagtcaa
      481 tctgcgtctg gattttttcc ggcacgccgc ccgggtaacg ctcgcgtgcg ccttcggccg
      541 tgagccggcg caggcactgg gcgggcgttt ccgcttcggg caccatgtcg cccagcgggt
      601 agcgatacag gtcgcggatc tcgtccaggc tgaagttgca acgcgccgcc accgccagcg
      661 tggcgcgcag cagcccggcc gggtagaggt cggccaggcg catgcgcgga cgcaaatgcg
      721 cttcggcatt ggcctgcagg gcaaagccgc actcatgcac ggtttttccc agcttgatgg
      781 ccgtcatcac gtcgtgcagc ggcttgcgcg aacgcacatg catgcgcacc ccgccggccg
      841 ccaccaggcg gatgccggtg cgctgcgcca ccgtttgcag ctgtagcagg cgcagcgcgt
      901 cgtccaggcc ttgcagcagc tcgacggcca gccaggcata catgccaaaa aggcctgcag
      961 cccaggcggc atgggcacag agcgcttctg tatcgatagc atccggcaaa ggcaacagca
     1021 gcacctcgca atcggccagc agcgaaaaat cgctttcgcc cagcgccacg cggtagctgc
     1081 ctttgtcgat cacgtcaccg gcctgtttgg ccgcggtgat gaactcgcac aggttgcccc
     1141 agccagtcgc gttgtgcggc agcaccacca gccggaacgg aggactggcc tgcacgaaaa
     1201 actccgcccc gggcagcagc ttgagcccgt gttttttggc cgccacatgg gcacgcacca
     1261 cgccggccac cgaacattcg tcggtgatgg ccagcgcgcg gtagcctaaa ccggcggccc
     1321 gttcgaccag ctcttcggca tgcgaggcgc cgcgctggaa gctgaaattg ctcagtgcat
     1381 gcagctcggc atagtcggga agcggtccgc tgaacatgac aattccaatc cagcaactca
     1441 ggcaaaaaaa ccgtgcaggt accaggcctg gcgctgcgta tgtgccggct gcggcaccgg
     1501 caaacgctcg ctgaaaatcc aaagcaaacc cgcctgctgg ctgcggtaga tgaagtagtc
     1561 gcggatcgcc gcgggcttgc ttcgcacacc cgtgccatcg ccggccgcgt caccggtcat
     1621 cagccagccg ctggcctcca gccgctgcgg gccggccagc cgaagcagcg ggccctggta
     1681 gaccgggctg tcgcccaccg ccgccagccg gagcggttcg ggcagcagcc aggtcgggta
     1741 gagggcatcg attctggccg cctcttccga tttcttcaag cctttctggc ctctggccct
     1801 tgtctggcgg gcgccaccag ctatggattg catagcgccc tgggcgctga cccagcgctg
     1861 catctgctcg ggcctgtggt cggcgcaggg ctgccaggcc tgcacctgcg cgggccccag
     1921 gcgggcgctc aggcgctcga tcagttccac cgcgctgtcg ccctgctggc gcgcttccat
     1981 cagcaggctg gtggtggcgg cggccgcgtc caccagtggc tcggtgacca gcgaacgcag
     2041 gctcagggca aacaccggcg ccggcagctt ttgctgcgcg agctgctcgg cgatcaggcg
     2101 tgtgacgtgg cgcaggtcct gcgcgggctg ggcggtgcgg acttccatct cgccggtggg
     2161 cgccacgtcg cggcgcttgt ccaggtgcca ggtgagccgg agtgcgcaga tgccgctctg
     2221 gcgccccagc agccaggcgt gcaggctggc cagcaggcgt tcggccccgg ccatcagcgc
     2281 cgggccgtgc gtgaccagcg cgttgagctc gattttttcc tcgaagtgtt cgggcagcac
     2341 cagccagtcg tggttgtcgg gcaggctgcc gcgcgcgcgg tccagcgcct ccagcagttc
     2401 cgcgccgaaa cgccgggcca cgccgtcgcg cggcagtcgc agcaggtcgt cccagctgcg
     2461 gcaaccgatg cgctcgagca cgcccaggtg cgggcgcgcc gcagtcaagg tgtgcatggg
     2521 caggtcggcc acccgcgtgc ggccctgccc cggcttgccg gccgcgtgac gcaagcgccc
     2581 cagtgctatc aaggatgtag cgccttgcga acgccgtata aggggtaccg cccgatttgc
     2641 ttcaaaaaat tcgatcaaac gctgctccag cagctgcatc agccgcgcca gcccgccaaa
     2701 caggcgcagg ctgccggtca cgtccagcag cacggcctcg tcgaccagcg ccacgcgcgg
     2761 cgtgaaaccc agcgccaggc tggccacgct gcgctgcagc gcggcctcgt ccatcaacgc
     2821 cggccccggc tcagtggtga tggctgtttg caatgcgatc cagcaaggca tgcacctctc
     2881 ctgatgcggc ggaaacagcg gccggggccg gctgcagcct gggcagcagc acaccggctt
     2941 cctcgcggcg gcggcgcacg cgttcgcggc tggccgccag caaggccgtc aggcgcgccg
     3001 gccgggtggc cagcagcagc ggcgcggcca gcggcgggcc gcgccgcttg agcacgtgca
     3061 cccgcaggtt gccgtcttcg gccgccgcac cttcgagcag caggcgcagc ggcgcgggtg
     3121 aagactcgag ctgcgcacgc agaggccgga acacaaacaa cagctggctg tgcgcatgcg
     3181 cggccatctg caggcggcgc aaatgggcac tgcgcgcatc gggcagccag gccagcacgg
     3241 cggccacgtc ggcacactgc agcgcttcgc gtgtggccca cagcagcgcc ggcccctggc
     3301 cgcgcacgca gagcacgcgc tgcatgttga gctggcgcgc gcccagcgcc gggccaaacg
     3361 gcaaccaggg tgcgcccacc agcaccacca gcttggcggg cgccgcggcc tgcacggcgg
     3421 ccagtgcagg cgccagcaga ccccactcgt gcaggccggc ctgcggctgc agcatttcca
     3481 ccagcgcgcc ctgcggccag ccgccgccgg gcaacacctg gttcagcgcc gcatacccgg
     3541 tgtccagggc cggcaggctg tggcagccca gctcggcggc gcgccagacg tcggcgtgtt
     3601 gcgccaggcc ggcgggcatg gcgtccagcg cccggcccgc agacccaggc ggcgtggaca
     3661 tggcggaaga aagcgggttc atgaaggaaa aagacataaa atactgttga tatgtacagt
     3721 attacacctt ttccggcctc agaggagaat cctttct
//
LOCUS       DAIDFG010000303         3731 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_303_length_3731_cov_16.979053, whole genome shotgun sequence.
ACCESSION   DAIDFG010000303 DAIDFG010000000
VERSION     DAIDFG010000303.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_303_length_3731_cov_16.979053"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          739 a         1198 c         1171 g          623 t
ORIGIN      
        1 gaccagcgac tgcttgtggt ggtgcacaaa ctcgatcgga tcctcaatgg tcaggatatg
       61 ccccggcacc gtgccgttgc ggtagtcgag catggcggcc agtgtggtgg acttgccaga
      121 acctgcggca cccacgacca ataccaggcc tcgcttgaga atggccaggc ccttgagagc
      181 aggcggcaac ttgagttcat ctatcgatgc gacggtcgag gtgatgtagc gcaccaccat
      241 cgccacctcg ccgcgctgca tgaaaatgtt gaagcgaaag cgccccaagc cgtcgatggt
      301 tgtggccagg tcgcactcca gcgatgattc gaactcacgc atctgggact cgcgcatgac
      361 cgaataggcc agcgacttcg catcgccaga cgtcaaggcg ggcagattca tgggacggat
      421 caccccatgt accttgacag atggtggggc acctacggtg acaaaaaggt ccgaagcatc
      481 ggacttcacc atgaactgca gatagcgccc catgtcgttc aggtcattcg atgagatggc
      541 gtccatgacg ggcgttcctt gttgcagcgg aatttgcagt ctaccctgca tgggcctgcg
      601 tcaaccggca ctccacgcca ttttcatcgc catccgcgcc accgtcgcgc gttgaccatc
      661 gaccggaacc ccaggtcagg caatcgcgat accgcaccac tcggcaaagc ggtggaaggc
      721 atccgcccag agcgcccagg cagccgcgag gaccagcagg ccaccggccg cgcgtgtgcc
      781 ccagtcctgc ttgaacttgt taccgcgccc ctgaagcttg tcgtacagca gcggcacgag
      841 catcagcgac acgctgctgc cggcgacaaa cagcgccatc gacagcgcgc cctccagcgg
      901 cccgccgctg agcgaggcca ccagcagcgc cgaataaagc aggccgcagg gcatgaaggt
      961 ccacagcaca ccagtagcga agatgccgcc gcgcgccgtg gccagtggcc ggatgcgcgc
     1021 ccagacggcg cggccagcgt cgctgaccca tgccggctgg cgggcctgat acagcagcat
     1081 caacccccat agcagcacag ccagatgaaa cagcgtccag accgggcgca acgccgcggt
     1141 gttggtggtc aaccaggcca ggctctgcac ggccagcgcg gcagcggcgc cggccagcga
     1201 gtagcccgcc agacgcccag cctgaaagct ccaaagtgag cgcgacgacg agcccgcacc
     1261 ctgacgcggc cccaggcgcg aaatgcccgc acaggctgca ccgcacatgg cagcgcaatg
     1321 cgggccgccg gccaacccca tcatcagggc tgttacagcc agtgtggtgg acatggcgga
     1381 aacctcttag atgatgcgcg aaaacctggc gcggtttcgg tcggcctgca ggtagcggtc
     1441 gaacaccatg gccaccgcgc gcacgaagaa ccagccctgc gccgtgacct gaatgccgga
     1501 ctcgtccagc agaaccagcc cggcatcggc cagactgcgc agctgttcga gctcggccgc
     1561 gaaataactg cggaagtcca gcagccacgc cagctcgatt gactcgaaga gcacctggcc
     1621 ctggcacatc agtgccatga tcacggcacg gcgcgccaag tcatcgcggt tgagcgccag
     1681 cccacgcacc accggcaggc ggccctggtc cagatggtcg cagtactctt ccatcgtctt
     1741 ggcgttctgg ctgtaggtcg caccgattcg tccgatggcc gagacgccca ggccgatgag
     1801 gtcgcagtcg ggctgggtgc tgtagccctg gaagttgcgg tgcagccggc cctggcgctt
     1861 ggccacggcc agcgcgtcat tgggcagggc aaagtggtcc atgccgatat agacataccc
     1921 tgcacccatg aaggccgcca gcgactgcgt cagcatggcc accttggccg cagcaccagg
     1981 caaatcggcc gtcgtgatgc gtcgctgcgg cttgaagcgc tcgggcaggt gggcataggc
     2041 atacagcgca atgcggtcgg gccgcaactg cgccacctgg gccagtgtgc gtgcaaagga
     2101 ctcgggggtc tggcgcggca ggccgtagat caggtccaca ttgatggaat cgaagcccac
     2161 ctcgcgcgcc tgggccatca gttcgctgac ctgctcaaaa ggctggatgc ggtgcacggc
     2221 cttctggaca tcgggatcaa aatcctgcac gccaaaactc aggcgattga aacccatgtc
     2281 ggcgagggcg cgcagccgtg tgccgtcaac cgtgcgcggg tcgatctcga tggagtattc
     2341 gccgccgggc accatcgtga aactgcgccg cagcatggcc atgagttctc tcaactcttc
     2401 atcggacagg aaggtcggcg accccccgcc cagatgcagt tgcgtgaccg tctggccctc
     2461 gccgagctgg gccgtgtgca ggtcgatctc gcggctcagg tagcgcaggt agctgatggc
     2521 acggttatgg tgcttggtga tgatcttgtt gcaggcgcag taataacaca gcgattcaca
     2581 gaacggaata tggacataca gcgacagcgg catggtcagc ccggccgggc cggtgcggcg
     2641 ctggaccagg gcctgaccat agtcggctgc ggtgaaggct tctacaaaac ggtcggccgt
     2701 tggataggat gtataccgcg gccccggaac gtcgaatcgt tggagcagat cgggcgatac
     2761 attggtcatg acatgattct cgtggaggta agtgactgtg ccgcgtccgc ccccctgacc
     2821 gtttgatatg gatcaaatgc gccggggcgg aggacaggac aatttgatca agatcatata
     2881 aggctctccc atgaaggaaa tccccgtacg tacgcccccc gcggatctgg taatgactgc
     2941 cccggaaacg ccgtttatcg atgaaaaaac tatcaaggtg gcgtgctcga actgcaacct
     3001 tcgcgaactg tgcatgccgg tgggcctgag cccgcaagag ctcgcgcgca tcgacgaggt
     3061 cgtggccatc cgccgcaagg tcaaacgcgg cgccaccctg tttcgcaatg gtgagccgtt
     3121 cgaatcactg tttgccattc gcaccggctt tttcaagacc tgcgtcactt ccggcgacgg
     3181 tcgcgaccag gtcaccggct tccagatggc cggcgaggtg atcggcctgg acggcattgt
     3241 caatgaccgc catacctgcg atgccgtcgc cctggaagat gccgaggtct gcgtcatgcc
     3301 tttcaatcgc atcggtgagc tctcgcgcga gatcaatgcc ctgcaacacc atgtgcaccg
     3361 catcatgagc cgcgagatcg tgcgcgaaca cggcgtgatg ctgctcctgg gcagcatgcg
     3421 ggccgaagaa cgcgtggcgg ccttcctgct gaatctggtg cagcggctgc atgctcgggg
     3481 gttctcgggg tcggagctgg tcctgcgcat gacacgcgag gagattggta gttacctcgg
     3541 gatgaagctg gaaaccgtga gccgcacctt ctcgaagttt gccgatgacg gcgtcatcga
     3601 ggtccaccag cgccatgtgc gcatcctcga ccccgccgcc ctgcagtcca tcgtcaacca
     3661 gcagggctgc aactagggcc cccacgcttg tcgcttcgcg tactgcgctg ccccccgagg
     3721 gggcgctgcg c
//
LOCUS       DAIDFG010000304         3677 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_304_length_3677_cov_17.934843, whole genome shotgun sequence.
ACCESSION   DAIDFG010000304 DAIDFG010000000
VERSION     DAIDFG010000304.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_304_length_3677_cov_17.934843"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          651 a         1218 c         1183 g          625 t
ORIGIN      
        1 atctccttgg ccgctgccgc gcttctctgc gccaggcttc gcacttcagc ggccaccacg
       61 gcaaagccgc gaccctgctc accggcacgc gccgcttcga ccgctgcgtt gagcgcaaga
      121 atattggtct ggaaggcaat gccgtcgatc acgccgatga tgtcgacgat cctgcgagac
      181 gaggtgttga tcgcacccat ggtgcccacc acctcggaaa cgacgctgcc cccgcgcacc
      241 gccacgccgg cggcagacac cgccagctgg ttggcctggc gggcgttgtc ggcgttttgc
      301 ttgacggtgc tggtcagctc ctccatggcg gcagcggtct gctccaggga actggcctgc
      361 tgttcggtgc gtgccgacag gtccatgttg cccgcggcga tctgggcggc ggcggtgacc
      421 acgccgccgg agttggccgc gatgtctgcg acgatcgccg tcagattggc gctcatctgg
      481 ttcagggcgc gcaacagggg tttggcctcg tcagccggct ggatctcgaa cttcatgagt
      541 tcgccgctgg caatgccctg ggccacgtga acggccttgt gcaacggacg aaacaccgaa
      601 cgcaccagaa aatagcccag ccccgcccac gccatggctg aagccaccgc caccgcggca
      661 aatgcgtcgg gcagcctgcc ggcctcgccg gccgccgcaa caagccggct gatttcgcgc
      721 cagcccagga cgcccagcac cagcgtcagc acggttgcca gggcggtgac ccagactatc
      781 ctctgctgga tgggaattct tcgcacgctg gccagggggc tgcgccagac cggcgaaatc
      841 acctcgccat gctggatcgt caacccgccg gcatgcccct ccctgatatt tctgtagatc
      901 tgctcggcct gggccacctg ggcggcttcg ggcttgctgc gcaccgacat gtaacccagg
      961 acctggccgt ggctgcgtat gggcgtgacg ttcgcctgca cccaatagaa gtcaccgttt
     1021 ttgcgccggt tcttgaccag gcccgtccac ggggaaccct gccccagggt ctcccacatg
     1081 tcggagaaag cttcaggcgg catgtcggga tgccgcacga tgttgtgggc ttgacccatg
     1141 agttcgtcgt tcgcataacc gctggcctcg acaaacgcgg gattggcgta agtgatacgt
     1201 cccttgaggt cggtgcggga gaccagtgag acgtcctccg ccagttcata agcaatgttt
     1261 gtagttggca gattgagacg catggcgggt gaaaagcttc ctttgaggcg ttgaaggtgt
     1321 caatacgacc gaatctccca tctaacggca gatccggcga aaaactttaa taaacgcctg
     1381 ccaacgcatc ccgccaaggg cgctggcggc aagccagcag acccacttgc cactcaccgc
     1441 tcaccgctca ccgcagaatt caagggcgca ggccatccgc tacggtcggg taatgcagcc
     1501 gcaggcccag ctcccgcttc agccgcgtgt tggctagccg gcgcgactcg ctcatgaagc
     1561 tcagcagcat caacggcaat tcgtcctgcg cggtgctgcg tgccacccgc ggcgggcgcg
     1621 gcaggctgta caggtcggcg gcgaggtcga agtagtcgcc catcttcagc tcggtgtcgt
     1681 cgctggcatt ggtgatgcgc tggggtttgc cgcgccacag agccgccacg caggcacgcg
     1741 ccaggtcgtc ggcatgaatg tggttggtga acacatcgtc cgctggcgcc agcaccggcg
     1801 tgcccttgag cagacggccg tgcggcgtgc cgccctcgcg gtcgggggcg tagatgcccg
     1861 gaatgcgcaa cgtgtgcacc cgcactccgc tggaccggcc aaaaaagcgc agctgggtct
     1921 cggcatcaac acggcgccac gcgcgcggcg tgtgcgggtt cacggcgcgc gtttcacttg
     1981 cccactcccc ctggcaatcg ccgtacacgc cgctggtcga gccatacacc accgcttgcg
     2041 gcaggccgcg caggcgcatg gcccgcagca gggccagcgt gcgcgggtcg cggcgccagt
     2101 cgggcaggtt ctctccgggc ggcggggcca ggtggaccac gcgggtggcc agcccggcca
     2161 gccgccgcag ggtggcgctc tgatccaggt taccggccag cggcgtgatg tgttgggcac
     2221 gcagttcagg gctgcgttcg ggcgacgagc tcagggccag cagccgcaca cgcggcgcca
     2281 gctggcgtgc cacacgcagg ccgacgtcgc cgcagccgat gatcagaata cgctggcggc
     2341 gaaagcgcga aggcagggcg ccgaggggac tttggtttga gggcaaaatt ggggaatctg
     2401 ctgaaaacag ccagagttat aaaaacagga ttacgtcaga ggataaccag tatgactttc
     2461 aacgtcaccg tgctgcccag cggccgttcg ttcaccgcca accccgacga agcgctgctg
     2521 gccgcggcca tccgccaggg cataggcatg ccttatggct gcaaggacgg cgcctgcggt
     2581 tcctgcaagt gcaaaaagat cgaaggcacg gtcgtgcacg gcccgcacca gctcaaggca
     2641 ctgtcgcacg aggaagaagc cgcggggttc atcctgacct gctgcggcgt ggcgcagacc
     2701 gacgtggtgc tggagtcgcg ccaggtcacc gacgaaagcg ccttcccgat caagaaaatg
     2761 ccggcccgcg tgagcagcct ggcacgcgcc tcgcacgacg tgatcgtgat ccgcctgcag
     2821 ttgccggcca gcgatgtgtt caagtaccac gctggccagt acgtggagtt tttgctgcgc
     2881 gacggcgacc gccgcagcta ctccatggcc aatgcgccgc acacgcagct ggacaacccc
     2941 ggcatcgagc tgcacatccg ccacatgccg ggcggcaagt tcaccgagca ggtgttcggc
     3001 accatgaagg aaaaagacat cctgcgcatc gaagggcctt acggcagctt cttcctgcgc
     3061 gaagacagcg acaagcccat ggtgctgctg gcctcgggca cgggctttgc gcccatcaag
     3121 gcgttgatcg aacacatgca gttcaagggc atcacccggc ccgccgtgct gtactggggc
     3181 ggccgccgcc cggccgacct gtacatgaac gactgggtgc tggccaaggt cgcagagatg
     3241 ccgaacctga gctatgtgcc ggtgatctcc aacgcactgc cggaagacaa ctggaccggc
     3301 cgcaccggct tcgtgcacaa gtcagtgctg gaagacttcc cggacctctc gggccaccag
     3361 gtctatgcct gcggcgcgcc catcgtggtc gactcggccc gcgcagacta cagcgcgatc
     3421 ggcggtttgc ctgctgaaga gttttatgcg gattcgttta ccactgaggc ggacaaggcc
     3481 aaagcgctgg cgtgaatttt tgcgctgctt gcgtcacgct gcgccttcag tctgctctgg
     3541 acagtaggac ggactcccca ctcccacccc tatcccctgc cctcgccccg ctgggcgatg
     3601 gagggggact tcatttggcc gtgtgtgctg caagcgcgtg caaacatgac ggtcccgtgg
     3661 tccgacctga cgcgctg
//
LOCUS       DAIDFG010000305         3669 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_305_length_3669_cov_17.828998, whole genome shotgun sequence.
ACCESSION   DAIDFG010000305 DAIDFG010000000
VERSION     DAIDFG010000305.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_305_length_3669_cov_17.828998"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          586 a         1139 c         1223 g          721 t
ORIGIN      
        1 gtgaggggag ctggtccatt atcgggctcg cggcggcgtg ggcgtcgttt tgtctgtcac
       61 ccgacgtatc atggcctcgg ggcgcagggc gtcccgaagc cttccgtaca ggcagccacc
      121 cctcaagcca actcccatga gacatccgga cagcaacagc acgaaacagg cccgcccggc
      181 cggtgcgggc cttcggtggg gaccattggt gatcctgctt ttctggctga cggtcatggg
      241 ggccctgtac gccgtgatgc gccaggtatt gaagccgcca ccgctgaccg tttcagtgac
      301 cggagaactg gtgattccgc gcgcccggga cggccacttc tacgcgctag gacaggttca
      361 tggccggcct gtcacttttc tggtggatac cggcgcatcg ctggtggtgg tcagccagga
      421 gtttgcgcgg accgccggca tggcgccggg cgaggcgacc accttccgca ccgccagcgg
      481 cgagctgcag ggccggattg tcaaaggtgt cacagtgtcg gtcgggccgg ccagcgtctc
      541 gggcacacgg gtcggcgtgg ggctggtggg gcccgatccc tcgatggccc tgctgggaca
      601 aagcttcctg tcccggttcg aaatcgtgct gtccggggac aacatggtgc tgcgcggcaa
      661 ataatgaacc taaggttttc attttgcggt gaacccgcta caccgataag tctcagattc
      721 tccccctggg ccgccacgct gaaaaccgga cggtaaataa gttaccgcta ccccggtaat
      781 tgatgactga cgccggcgca gcgcaatcct aggatgacat ctgttcagct cttccggctg
      841 atggcaggag attctcatga tgcgagccct catcaccctt ttgatcagcg tgtttgccac
      901 tgcatgcctg cccgccgcgg ctgctgatat ctcggcctac cagcgtggcg tgttgatcca
      961 gggaaccatc gagaaaggtg actacgccag gatcatcggg ttcgtccggc accccgacaa
     1021 ctacgggcgc ttcgcgcgca tggtctacct ggattcatcg ggcggtgatg tggtggaggc
     1081 catcaagatc gcccacctgc ttgacaagag ttacgccgcc acccatgtgg cccgtggcgc
     1141 gcgctgctac agcgcctgtt tcattttatg gatgagtggc gtgacccgcc atgtgtccag
     1201 cggcgcaacg ctgggcgtgc atcgcatcca tgtccagctc aaggagggca gcatcgacca
     1261 gcagtcggcg tatgtcagcc agattgcgcg cacggtggaa aactacatgt cagggctggg
     1321 cacgccgcgc ctcattctcg acaagatgaa cgccactgcg ccgacagacc tgtttgtcat
     1381 cagcccgcgc tggctggccg agcaacagtt gctgaccgcg gtgtcttacc ggctcggctt
     1441 catcgatgca gcgcaggccc gctgcgggcc cgaacctgtg gcgctggcca tgaagggcaa
     1501 ggcgccgccg gccgatggcg aggcggaagg ctgggtctcg cagatcaaga gctggatcac
     1561 ctgcgctgac gagttgcgtg ccagcaacca gagaggtgat ggcgtgacca tcgccgcgct
     1621 gatcggggaa taactaagca cccacgcttg tcactgcgtg tactgcgctg ccccccgagg
     1681 gggcccctgc ttgtttgaac cccggcgaac aggcgtcgac agctcggaac gagcggtata
     1741 agcggtcgta ggtggggctt cgctccagac cagccagggc cgcggaaccg gcttagccgg
     1801 gccgcaggcg cagcggcccc ctcggggggc aggaagctac gcgaagcgag cgaccgtggg
     1861 ggccttactt gggggcttta atttccgtcc cgtatccact gcgcggcttt ttccgcaatc
     1921 atcagggttg gtgaattggt gttgccgctg gtgatcagcg gcatcacgcc ggcatcgacc
     1981 acacgcagcc cggtcacccc gcgcacccgc aggtgcgagt ccaccaccgc catggggtca
     2041 tcggccctgc ccattttggt ggtgcccacc gggtggaaga tggtggtcgc aatgtcgccg
     2101 gccagccgcg ccaggtcgtc atcgctctgg aactgcacgc cggggcggaa ctcttcgggc
     2161 tggtagccgg ccagggcgct ctggctcaca atgcggcgcg tgacacgcag ggagtcggcc
     2221 gccaccttcc ggtcctcggt ggtgctgagg tagttgggcg caatcgccgg cgcgtcttcg
     2281 aagttgccgc tcctgatctg aaccgtgccg cggctggtgg ggttgaggtt gcagacgctg
     2341 gcagtgaagg cgttgaagtt gtgcagcggc tcgccaaacg cgtcgagcga cagcggctgc
     2401 acgtggtact cgatgttggg ccagggctgg tccggcgagc tgcgcgtgaa ggcgccgagc
     2461 tgcgacggcg ccatgctcat cgggccggtc tgtttgagcg cgtattcgag gccgattctg
     2521 gccttgcccc agagcgagct gcccagagtg ttgagcgtga agttttttgc cttgctgccg
     2581 gcgatgttgt tgaccttgta gaccgagcgg atctgcaagt ggtcctgcag gttggcgccg
     2641 acgccgggca ggtccaccac caccgggatg ccgtgctgtt gcagcagcgc cgccgggccg
     2701 atgcccgaga gctgcaggat ttgcggcgag cctatgctgc cggcgctcag gatgacttcg
     2761 ccgagcttgc cctgcgctct tgctgagtcg cgcgtggcaa aggcctgcac gcgttcgtgg
     2821 ccggtccaga cctcgacgcc ggtgcagcgc tggctgccgt cgtcctggcg ctcgacaatg
     2881 agtttgcaga cctgcgcgcc ggtccacatt tcgaagttgg ggcgggcaaa acaggtcgga
     2941 cgcaaaaacg ccttggcggt gttccagcgc cagccgcttt tctggttgac ctcgaaataa
     3001 ccgacgcctt cgttgttgcc gccgttgaag tcgtcggtgg ccggaatgcc cgcctgctgc
     3061 gccgccgcgg caaacgcgtc gaggatgtcc cagcgcaggc gctgcttttc aacgcgccac
     3121 tcgccgccca tcttgcgtgc caggccttcg ctggtggcgt gctggctttt gtggccatgc
     3181 agtcgccgga agttttcatt gaccacgacc tctgccttgc ctttgggcac atcgagtttg
     3241 tagtggtcct cgtgtttgcg aaagcccggc agcaccttgt cccaggccca gtcgtcgtcg
     3301 ccggtgagtt cggcccagcg gtcgtagtcg cgcgcctggc cgcgcatgta gatcatgccg
     3361 ttgatgctgg agcagccgcc cagcgtcttg ccgcgcgggt aacgcagcgt gcggccgttc
     3421 aggccggcgt cgggttcggt gttgtagagc cagtcggtgc gcgggttgcc gatgcagtac
     3481 aggtaaccga ccgggatgtg gatccagtga tagtcgtcct tgcggccggc ctcgatcagc
     3541 aacacacgtt tggacgcgtc ggcgctcagg cgattggcca gcaggcaacc cgcagtaccg
     3601 cccccgacga tgatgtagtc aaagatgttt tcgttcatgc gtcttgtctc gtcgttcttg
     3661 tagccatac
//
LOCUS       DAIDFG010000306         3668 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_306_length_3668_cov_13.303626, whole genome shotgun sequence.
ACCESSION   DAIDFG010000306 DAIDFG010000000
VERSION     DAIDFG010000306.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_306_length_3668_cov_13.303626"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          752 a         1089 c         1222 g          605 t
ORIGIN      
        1 ggttttggcg cactggacga tgtccacacg cgtgcgttca gcttccatgg tgtttttcaa
       61 tcagggtgcc gatggcctgc aggccccaga gcacggggag gatgacgaga agaaagatga
      121 gcgtgtaggc cgagccgatt tcaagccgca tggaggcata gctgtcagcc aggcctacgg
      181 gtagagtgcg cgtcagtggc gtgtgcagca tccaggtgag gttgaactca ccgaccgaca
      241 gcgtgaagac catcaggctg ccggccacaa tcgcaggcag cacggcgggc agaagcacac
      301 ccacaaagcg ctgcatgaag ccggcgccca gcgatctggc agcctcttcc agcgagtgca
      361 gttcgggccg ctggaaagcc gacgataccg tgcgcaccat gaagggcaac gtgaacacca
      421 tatgacccac caggatgaat gcaaagcttt gccggaaccc actgatctgc ccataggcca
      481 aaatgagcgc aagcgcggtg gctaggccgg gcacggctac gggaagcgtc aacaattctt
      541 caaaaatccg ggcggcccgt gagcggctgc gggccagcgc atacgcacac ggaatgccga
      601 tgagcaggtt gcccagcaca catagcaggg ccagcaccag cgaggccgca accgtgccac
      661 cataaagctc ccatacctca cccagccagc gcagcgtgag cccgcttttg aggccggcgc
      721 tgtagttgtt gaccaggccc gcgagcacgg aaagcgcgat gggcgcgatc atgaacacag
      781 ccaccagcgc tgtcagcgcc atcagcaagg gggagacctg gcgaggggtt ctcatggcat
      841 caagctcccg ccccggccgg gccggcaacg cgccgcgaca ggaacaggac cagccaggtg
      901 ataagaccca gcgtgatcga caggctggca gcgagtgcaa agttggcgta gttggtgaat
      961 tcgttgtaaa tggtgatggg cagcacctcg aacttgctcg ccagtgtgaa ggcggtgccg
     1021 aaagcgccca tggcggtggc gaacacaatg gcgccgcagg caaacgtcgt ggggcccagc
     1081 tcgggcagcc agacatcgcg caccacccgc cagcgcgatg ctcccagcga gcgtgcggct
     1141 tcctccaggg gaaggtccat ggcgctggca gcggccgtgt aactcacgat agcccgcggc
     1201 agcgagaaat acaggtagcc cagaaagagc cccgccaggc cataggcaaa ggtgatgcgg
     1261 cccaggccca atgcgtcact gaggtcggcc accaggccct gccggccgcc cagcaggatg
     1321 atgaaaaagc cgatgatcac gcccggaaaa gacaatggca aggtcagcag cgaaagcagc
     1381 agctttcgcc ccgcaaactg cgaccttgcc agcgtgatcc cgaccagtgc gcccagcacc
     1441 agcgtcaccc cggtcaccac cagcgacagg cccagcgttt gcaacaggct ttgcaggtag
     1501 cgggtgttcg tcagcaccac aaagtaggtg gaccacccca gcttgcccgg caatgccagg
     1561 agctgcactg ccggcagcag ccaaaaggcc gcgaagaacg ccaccgccgg cgctgaacag
     1621 gccagcagca ggcggcgcga aggcgcgaag gaggaagaac tcatggcctg ccgatgtaac
     1681 gcccaaggct tactgggctt ctttcaggta acgatcggaa aagccctttt gcacggcggc
     1741 catgcgggcg tagtcaacgc ttttggcacg ggcgtattca ctggccggca ggaagcgcga
     1801 ctgggcttct ttggagatcg cgctggcgcg caccgggcgc aggtaggcat tggcccagat
     1861 ggcctgacct tcgtcggaca gcacgaagtc cagtactttt ttaccgttgt cggcatgggg
     1921 ggccttggcc accaggctca tcacgtaggg gaccacgatg gtgccttctg ccggaatcac
     1981 gaactcgacg ttagctttgt ccttgtattt ggcgcggtaa gcattgaagt cgtagtccag
     2041 caggatggcg atttcaccgg acagcatgcg cgcgtaggag gtctgcttgg gcacgatggg
     2101 ttcgtttttc tgcagggcct tgaaatagtc gatgcccggc gtgaagttgt ccagcgtgcc
     2161 gccgcgcgcc tggttgacgg ccaccgcgcc cacatagccg acaaaagctg atgccggatc
     2221 gaggtagcca atcaggccct tgtagtcagg cttgaggagg tcggcccatg aacggggaac
     2281 aggtttgccc ttgagcgcat ccacattgac catgaagccc agcgtgcccg aatggatggt
     2341 gaaccagtgg ccttgcggat ccttgagtcc ctcgggaatg tcgttccagg ccgctggctt
     2401 gtaggtgctg accacagcgt ctttttgcgc ctggatggcg aaggtgacac ccagataggt
     2461 cacgtcggcc accgggttgg ccttttcggc aataagctgt gccagcgatt ggccggagtt
     2521 cttgttgtcg gggggcacgg tgatgccggt cttggctttg atggctttca gttgggtacc
     2581 ccagtcggcc cactcgggcg ggcagttgta gcagatggcg gtctgggcca tcgcggtggc
     2641 gccggcggcc aggccaaggc ccagcactgt ggcttgggca aggcgttgca gggatgtttt
     2701 catgtgagct ccgttcaata ggttaggaca cgaggacgtt gaggaaatac cagggaaaca
     2761 tgcaaaaaaa gttcagtctg tgttcagata tgccgcgcaa gactcgcctt cacggaagcc
     2821 gtaggccagc gagaggctgc tttcaggcgt cagggccgag ccgccggtca gcgcctggac
     2881 cagcagctcc acactgcagc gcccgatgtc ggcattgggt tgggtgatgg tgctcaggat
     2941 gggggtgagg tcctcgccca aagcgatgcc gtcaaagccg atcacggtga ggtcccgcgg
     3001 caccgctagc ccggccaagt gggcggcgcg caggctgcgg atggccagca ggtcgttgga
     3061 gcagatgagc gcactgggcc gcggctgccg gttcaggagt tcggtgattt cgctgacggc
     3121 ggtttccaca aagggcacct ccagcacctg cgggtctgct aggccggtgg cctccatgcc
     3181 acggcgaaaa ccgcggcaac gttgctgagc gcgatcggaa gcgccgagct ggccgctcac
     3241 cagggcgatg ttccgatgcc ccagaccggt cagccggccg acgagttccg ccacggcctg
     3301 ctcgctgtca accgagatgc aggggtggtc ggcgtgccgg ttgtacgcca gcacataagg
     3361 aaggcgcgtg acgcaaagtc gctgcagggc tttggaggtc tccgggctgg aaaccaccag
     3421 gatcatgccg tcaacattgc cggccagaag caggtttacg gcctgctctt cctgctcgag
     3481 ccggtagtcg gtggtcagcg gcaggatggc atagccggcg cgggtggccg agtgcgcgat
     3541 gccttcaagg cactcggcaa acaccggatt caacagggtg ggcagaacca cgcccagcac
     3601 gcggctgcgt tgcgtgcgca aggtgcgcgc gctcgcattg ggcatataac caagcgcaag
     3661 cgcgactt
//
LOCUS       DAIDFG010000307         3664 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_307_length_3664_cov_18.578831, whole genome shotgun sequence.
ACCESSION   DAIDFG010000307 DAIDFG010000000
VERSION     DAIDFG010000307.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_307_length_3664_cov_18.578831"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          661 a         1151 c         1211 g          641 t
ORIGIN      
        1 cgtgatggtg gtgccgctga ttctgaacgc ggcaggccac gtgaagatgg acattccgca
       61 ggccattgac gcggcgcgcc tgtgttttcc gcaagtgcag ttcctgtacg cgccgcacct
      121 gagcgcctgc gatcacattc tcagcgtggt aaagcgccgc ctgaaaggcg cgatgcaggc
      181 gctcgacatg ccggacccca ccaccaccgg cgtggtgatt ttggggcgcg gctcgtccga
      241 ccggcaggcc aacggcgaga tggccaagat ggcgcgctgg ctgatggagg aaaccgacca
      301 tgaactggtc gacctggcgt tcaccggcat cacctggccg cgccttgaaa aaaccgtgca
      361 gcggcaaagc ctgctgggca tgacgcaagt ggtcgtgctg ccgtactacc tgtttaacgg
      421 cacgctggtc gaacgcatca ccaaacaagt ggctaacctg aaagcccagt accccgccat
      481 ccgctttacc tcgaccgcct atttcggttt tgagcgcgag atttttgaac tgctggaaca
      541 acgggtgaat gacctgaaac gcggcgcgcc cgagtcgcgc atgccctgtg acggctgcaa
      601 gtaccgtgag tttggcgtcg aaaacggcat gggggggcat caccatgatg atgcgctgcc
      661 gcaccctcat gatcacgcgc acgaacatcc tcacccgcat gcaccggtca tcgttcaaat
      721 tcatccgcag cctcatgcgc acgaccacac ccatcctcac gagcacgccc atccatgagc
      781 accgccaacg tcattaccga acagctcacc gcggcgggcc gggccattga acacgattcc
      841 tttgccgtga ttgaccgcga ggttgcatcg catgcctata caccggccca gtggcccatc
      901 gtgcgccgca tgattcacgc caatgccgac ttcgacttca acggcctgac cgactttcat
      961 ccacgggcgg ttgaggccgg cgtggccgcc atcctgtcgc gcagcacccg cgtggtggcc
     1021 gatgtggaaa tgatctgcgt gggcctgtca gcgccgcgcc tgggacattt cggcatggcc
     1081 acgcaccagt tcatctcgga cgctgacgtg attgaagccg ccaaagccga aggtacgacc
     1141 cgcgcggtgc aggccatgcg caaggcgcac cgcctgggcc tgatcaatgg tgccatcgtc
     1201 ggtatcggca atgcgccgac cgccttgatt gagctggtgc ggctggttca tgaagaaggc
     1261 gcgcgtccgg ctttggtcgt gggcatgccg gtcggttttg tgtcggcggc cgagtccaaa
     1321 gacctgatgg cgctggagaa cgaggtgccc tgggtggtga tcagaggccg caagggcggc
     1381 tccacgctgg tggtggcggc cattcacgcc ctgctgggtc tggcagaggc gcaagagctc
     1441 acggagaaaa cgtcagcatg atggagaaaa atgcgccacg tggcacacgc accggtttca
     1501 ccaccggcgc gtgttcggct gcggcggcgc gggcggccac gctggggctg gtcaccgggc
     1561 aggtgccgga cagcattgaa tgcctgctgc ccaacggcga tctggtcacc tttgcggtgc
     1621 tggacggtgc ggtagatggc cacacggcgc atgccatggt catcaaggac gccggcgacg
     1681 accccgactg cactgacaag gcgcacctga ccgccgatgt gcggctgctg cccgaccagc
     1741 cgggccaggt gctgctcaaa ggtggcttcg gtgtaggcac ggtcaccatg caaggcctgg
     1801 ggctgaccgt gggcggcccg gccatcaacc cggtgccgcg tcgcaacatc gaggccaatg
     1861 tgcgcgccgc aggtagcgcg ctgctcgatg aggccggttt tgaagtgacg atttccgtgc
     1921 cgcaaggcga acaaatggca aaaaaaacca cgaatgcccg tttgggcatc ctgggcggca
     1981 tttccattct gggcaccacc ggcatcgtca agccctactc gactgccgcc taccgcgcca
     2041 gcgtggtgca gggcgtgcat gtggcgggca ccttgccgca tggtgtggtg gttcttacca
     2101 ccggcgggcg aactgaaaag ttcgtcatgg aggaaatgcc ccacctgccc gaggctgtat
     2161 ttgtgcagat gggcgacttt ttacgctacg ccatgggggc ggcggtgaaa gcaggcttga
     2221 agaaggtcgt gattggcggc atggtgggca agctcaccaa gatcgcgcag ggcgagacca
     2281 tcactcacgc cggtcgcgcc gatgtgaaca ccggcttgct ggccgatctg gccgcagaac
     2341 ttggcgtcgc gcctgatgtg tgtgaggaca ttcgctatgc caaaaccgcc cgctatgcgg
     2401 gtgagcgcat ggacgcgctg ggcttgggcc ccgctttcca cgcggcgctg gcgcagcgtg
     2461 tggtgaaaac tttacgtgaa cgttatcccg atcaattcga actgaaagtg ctggtctgcg
     2521 acttcgatgg tcgcaagata gcggaggccc cttgaattct gaaacgataa acctgattga
     2581 aaaatgccgg atcatcggcg tgctggacga tggcgtgaac agcctgagcc agagcgcgct
     2641 gacgcacctg aaagcggctc aactggtgat tggcggcgcg cgcacgctgc aattgctggc
     2701 cgcgcacatc gcgcccgacg cccagcagcg cgatttgacc ggggcgctgt cgcaagtgcc
     2761 cgagtggatt cgcgccgcgc aggctgcggg ccagcgtgtg gtggtgctgg ccacaggcga
     2821 cccgctgtgc cacggcatcg ccgcctacct ggcctcgcgc ctgtgcatcg aggccattga
     2881 ggtgctgccc aatgtctcca ccctgcaact ggcctgcgcg cggctgggcc tgccgtggca
     2941 ggagatgaag ttttcatcgg tccacagcaa ggatgcaggt gactgggtgg ccgggtccga
     3001 ccccggtcac ggcctgtacg cgctcttgcg cgacatccgc cagcatgacc gtctggcggt
     3061 gctgaccagc ccggacaaca cgcccgaccg catcgcccgc atgctggtca ccgaaggcct
     3121 ggccgatgat ttcgagatgg ccgtggccga gcgcctgtgc cagcccgagg agcgcgtcgt
     3181 cagcggcctc tccatcgcgg cggcggccag cattccgttt gccgacccca acgtggtgct
     3241 gctgtggcgc accaccttgc gcgcgccgca ggtgctgttc ggcctgcccg atgccagctt
     3301 tgagcagcgt catccggaga aaggcctgat caccaagaac gaggtgcgcg ccgtctcgct
     3361 ggcgcgcatg cagctgcgcg cggacagcgt ggtgtgggac atcggcgcgg gctcgggctc
     3421 ggtcgggctg gaagcggcac ggctgtgccg gcagggccat gtttacgcga tcgagaagaa
     3481 tgcggacgac agtgccatcg tgcagcgcaa ccgcatggcg atgggtatca gcaaccacac
     3541 gctggtgcat ggcaaggctc cggaaggact gcccgcgtgg gccgacccgg acgccgtctt
     3601 catcggtggc tcgggcggtg aactggccga gctgatcgta ctggccctgc aacggctcaa
     3661 gccg
//
LOCUS       DAIDFG010000308         3660 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_308_length_3660_cov_18.531761, whole genome shotgun sequence.
ACCESSION   DAIDFG010000308 DAIDFG010000000
VERSION     DAIDFG010000308.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_308_length_3660_cov_18.531761"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          613 a         1087 c         1236 g          724 t
ORIGIN      
        1 gaaggtgcgg ccgacctggg cgatgcggtc aatccaggcc ttgagctggc tggggatgga
       61 gaagttgtac agcggtgcac cgaccacaat cacgtcggcg gcgaggaact gggacaccag
      121 cgcttcggaa accgcgtttt cgcgcttttg cgcgtcgctc aggtcgctgc cggcgggcat
      181 gcggaaaccc agggactcgg ccgacaggtg gctgggggtt tccacggcca ggtcgaggta
      241 gctgacgtcg gtggccgggt gaccggcgcg ccaggaggcg acgatctggg cggtcagttg
      301 gcgcgacacg gagttgccgc cgaggatgct ggagtcgatg tgcagaagtt tggtcatgat
      361 gggttccttg agggggtgaa tcggggatgg atagcttgtt ggccgtattc accatgaatc
      421 gcagccatgg atagagtgtg cggatgaatc cattcattga taagacggca aaaatgcgat
      481 acatcgtcct gtttatagga caatcgacgt gaagaccccc ggctcaacgc cggggtgaca
      541 catcaaccaa ccgggaaccg gggagccgcc catgcaggac ctgaacgaca tgctgtattt
      601 cgccgaggtg gccgagcgcg gcggctttgc ggcggcaggc cgggcgctgg gcctgcccaa
      661 atcacggctc tcgcgccggg tggccgaact ggaagcccaa cttggggtgc ggctgctgca
      721 gcgcaccacg cgcaagctgt cgctgaccga ggcgggcgag ctctacctgc gccactgcgt
      781 ggccatgcgc gacgaggccc tggccgccgg cgaggcggtg gcgcagatgc agagcgagcc
      841 gcgcgggacg ctgcgcatca gctgcccggt gacgctggcg cagggcacgc tgggctacct
      901 gattccgcaa tacctggccc agcacccgca tgtgaagctc gacatgcggg tgaccaaccg
      961 ggtggtggac ctggtggaag agggcatgga cgtggcgctg cgggtgcgcc ccacgctgga
     1021 cgacagcggc agtctggtcg tcaagcaact cggcgcctcc tgcagctacc tgctggcgag
     1081 ccccgagctg ctcaggcggc agggtgcgcc agccacgccc gatgagctga aaaacctcga
     1141 cacggtggcc atgtcggcca acgaggggcg cagcgcctgg gaactggtgg gcccgcaggg
     1201 gcaggagttt gtgttgcagc accagccgcg ctacgtggcc gatgacctgc agacgctcaa
     1261 gctggcggtg ctggccggca ccggcatgag ctttttgccg gagatgctgt gcacggcaga
     1321 gctgcaggcc cggctgctgg tgccggtgct gcccggctgg gcattgcggc ccggcatggt
     1381 gcatgccgtg tttccctcgc gccgtggcca ggtgccggcg gtgcgcagct ttctggactt
     1441 tctcggcgaa cacctggagg ccgaggagct ggcttcctga atcgctcttt ttttgatagc
     1501 tggtggcgca agtgggacaa gggctaaggg ccgatttgat gcatatattt cctggcaagc
     1561 ggcgtgataa caccgcgcaa cgcgcgggtg tttgtggagg agggggcggg gatttgagcg
     1621 cgctggccca tcattgagcc ggaccgaacc tcatcgctga ccgcgcgcgc ccgttcggcc
     1681 tcaacttcgc ggttgcgcgg ttcagcgttg gtgaccaggg aaccgataag gtgtcgtgcg
     1741 gaagccgcca cctcttctac cgcgcgttcg aacgcggctt cattggcctt ggacggaacg
     1801 ctgaacccgc tgagcttcct gacaaactgc agtgaagcat cgcggatttc cagttccgtg
     1861 gccggtggtt cgaagttgaa cagggtcttg atgttgcggc acatggcggc tccttgagac
     1921 tgggggattt ttcacttgat gacgcagatg ccacatggcg tcaggccgcg ctggcatgac
     1981 gattgaataa aaaatcagat catgcgcggg ccggcgttcg gccggccatg tcccgcaatg
     2041 cagcgacctg ccaggagttc tccggctcga acgcctcgcg ttcagagact gctccgtacc
     2101 cgccatcggc gtagacagtc gcgtaggctg cataactcgc ggcctgtagc gcctttccat
     2161 tgatcgcatt ggccaccgcg tagcttgccg agacagccgc gtgaccacag ccatcgatcg
     2221 acttcgaccc tcgatgttga ttggccaggc ggtctgcttc cctttgcgaa tgttccaggg
     2281 cgtcacgact gacgcgaccc tccaggtagc ggcccagtac gtccaggcac tcgatgactt
     2341 ccggttcttc gagcaggtgt ctgatccgga tgacacaggc gtgagcgaag gccagccgaa
     2401 gccgctcgtt ttccggtgcc aaaaggcctg tttccgatgc aagttgctcc agcgcctggt
     2461 tcatgcacac ctccggtgac ggatggggtt tcgtcaattt ctggtttata tcagtctttt
     2521 tcaggccggc atgggcgctc gtcgggaaca aatccacccg ctgcgcgaag gcatcaacac
     2581 ctgacgcggt tcgccggtaa ccgggagggc tactccggat ttcccagccg gggaacttat
     2641 ctccgcctga acgcccgaac catcggacaa tccgtacatc gatcgaaaaa aaggaccctg
     2701 atgttgttga gctatgtcga aaccgcgccg cgccgtgtgc tggccctggt gtctgccgtc
     2761 tgcgtcgcca tgctggcgtt tggcctgtac ctgcagcatg tcgtcggcct ggagccttgc
     2821 cccatgtgta tcgtgcagcg ttatgcgctg gtgctggtgg cggtggtgac gggggccacg
     2881 gcttttgcgc gcggcaaagg gctgctcatg acggggtcag cggtgacgct gctggtggcc
     2941 ggcttcggcg cttttgtcgc cgcgcgccag agtttcctgc agtggtaccc gcccgaagtt
     3001 gtttcctgtg ggcgcgactt ttacggaatg atcgagacct ttccgctgca gcgtgtgatc
     3061 ccgatgattt tcaagggcag cggcgactgc accaggatcg actggacttt tctgggcctg
     3121 tcgatcgcca actggtcgtt tctctgtttt gccggcatct cgctgctgat gctggccctg
     3181 ctggtggtgc agggccgcag ggcgcaagcc gcacgccgct gatccggtcg ttcagggcgg
     3241 cagcggtgcc acccgcgccg gcaccagcac aatttcgatg ctgccgcccg ggttgtggct
     3301 tttgagccgc agttcttcat ggctcaggct caggatgtcg gttttgtact cctgcgcttc
     3361 ctcgatcatc gtgagcttgc cgttttggta ggtccaggca ccgaaggcgg ccttgctgtt
     3421 gggcgaggtg accaccagcg tgccgtccga caaaaacgcg tacagcgtgc cgggcgcgac
     3481 cccggtggac aggctgacct cccacacctt gttgatgaaa cccggcttca cgttggcggg
     3541 gtaaggcttg gacgtgtcgg ccaggtcgac gggttcggtc tctttcgagc aggccgcgac
     3601 cagcaaaaac acagccaggc aacaggcccg ccgcagtgaa tccttcatcg tgcgctactc
//
LOCUS       DAIDFG010000309         3637 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_309_length_3637_cov_18.357342, whole genome shotgun sequence.
ACCESSION   DAIDFG010000309 DAIDFG010000000
VERSION     DAIDFG010000309.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_309_length_3637_cov_18.357342"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          818 a         1116 c         1087 g          616 t
ORIGIN      
        1 aactcatata gcttggacgg cggcggggcc atgcggccag tcacgctgac cagtcccggc
       61 ggcgttgaca cagcaggcag gcgggtgcgg tcattgaagt cgcgcggcac ccagccacgc
      121 tgcaccagga tccacggccg cccgccttcc agcgccagcg gcgtcaccac ccagaaaccg
      181 gacttgccgc ccatcggccg gttgtccagg tacaccgtgt gcgcctcctg ccagacaccc
      241 tgcagcagcg cctgcctgtg caacaccgtc gccatgtcct gcgtcgccag caatgcgcgg
      301 ttatccaggg ccgggcgcct gttctgggca tcgatcgcag cctgcagcgc gatcttgtcg
      361 gcagcgcggc gccattgcca ctggcccagc gagaaggtca ggcccgccat cagcaaggca
      421 gccagggtca cgacccaaaa gcggacgctg ggcagggatg agctcatgcg cggataatcc
      481 ggttcatgaa ttacctcgtc gccattgcat ttctggccat catcgccagc ctcggatccg
      541 cactgttttt catgttgaag ggcggcagcg gcagcaaggc caaaggcggc aacatggcac
      601 gcgcgctggc ctttcgcgtc ggcttttcaa ttgtgctgtt tgtctgcatc ctgatcgcct
      661 ggaaaatggg ctacatccag cccaccggca tcgcggcggg caaataaaaa agaccgcaac
      721 agcggcctcc actcacaatt cggcgtagcg agagcttgtc agccgaggcg cgggtggccg
      781 gaaaccggag tggacttcag gtccatgagg atttccggac gggccccgca acgacggatc
      841 acgagctcgc agtagccgtc acatcacagc cagtagacga gaatgtacag gcccaaccag
      901 accacatcca caaagtgcca gtaccaggcc gcaccttcaa aaccgaagtg acggtctgcc
      961 gtgaaatgcc ctttttgcag gcgaagcgtg atgaacagca gcatcagcat gccgacaaac
     1021 acgtgaaagc cgtgaaagcc tgtcaacatg aaaaaggttg atccgtacac gccagacgag
     1081 agtttcaggt tcagctcgga gtaggcatga aagtactcgt agccctgcac acacaggaag
     1141 atgatgccga gcagcacggt catccacatg aacgcaatcg tcttgccgcg gtggttttca
     1201 cgcagcgcat ggtgagcgat ggtcagcgtg acgccggaac tcagcagcag ggccgtgttg
     1261 atggtgggca gccagaaggg acccatggtt gtgaagggtt caatgatgtc ggccggtgaa
     1321 ccggtcacgc cagcagccac actgggccag accgccttga aatcgggcca gagcaaggcg
     1381 ttttccaggc tgcccagcgc aggcaccgag tgcacacgtg cccaccacag ggccgtgaaa
     1441 aaagccccga agaacatcac ttccgagaaa atgaaccagc tcatgctcca gcggtaggac
     1501 aggtcgatct tgtggccgta aagcccgcct tcgctttcgc tgaccgcggc acgaaaccag
     1561 aaaaacagca cgcccagcca ccaggccagg ccaaacgcga gcgagtaagc gccccagccc
     1621 gcgccgttga tccactgccc cgcgcctaaa aagaggaaaa acaggccgat ggccgccatc
     1681 accgggtaag ccgaaggcgc cgggacgaag taatacgggg ttcctgcatg tgctgctgaa
     1741 gacatttcca actcctgttt cgctaaattc tcaaattcaa taatcaaagc ctggcggaca
     1801 ccctgcaggc tatttaccca ccacccaggt ggccagaaga atcaggccgc cgacaaaaac
     1861 aagcacccca accaggccaa ccacaacgat gtggaccggg ttgagctgct gcacatcctt
     1921 ctcgtagtca ccccgggtgc gcaggccgac aaacgaccag ctcaccgcct tgatggtgcg
     1981 ccagagggag cctttgcgct gcgtgaccgg cgtggtcata cgccggactc acgcgcgggc
     2041 cgcgccagat ccacggcggc aacaggcccg gctgccggcg cggtcggggt tttcccgccg
     2101 acttcaaaaa atgtgtacga cagcgtgata gtggtcacgt ccttggacaa cttggggtcg
     2161 atcacaaaca ccaccggcca ctgcttcttc tcgcccggcg ccagcgtgta ctggctgaag
     2221 cagaagcatt cgagcttgtt gaagtgcgcg ccggcctgca ctggcgcgta gctcggaatg
     2281 gcctgtgccg acatcgtgcg gttctgcaca ttctggaact cgtacatcac cgtcatcaat
     2341 tcgcccggat gcacctgcag cgaacgctgc gccggcttga acatccaggg accgcgtgcg
     2401 ttcgcgtcaa actcgaccgt gatgctgcgg ctcgtatcga cctgcgtgtt ggctgacagc
     2461 tttggcgtcg cgccaggaat ctgccggtcg cccagcgcca ggatgttgat gccggtcatc
     2521 tcgcagatgg ccttgtaaat cggcaccagc gcataaccga aggcaaacat gccaagcacg
     2581 atcacgccca gcttgcccac catccggaaa ttttcgcgct tcaggcccat gcgtcagcct
     2641 ttgccaaaaa agaccagctt ggccatgaag cccaggaaaa acaccaccgc aatcgaggcg
     2701 aagatcagcc ccagccgcag gttgttcttt ttctgttcag gtgtcatggc gcgcaggttt
     2761 tcttcgaacg gatcaatagc cgatcacttt ggtcgctgtg gcgtccaggc ggggcggtgt
     2821 ctcaaaggtg tggaagggtg cgggcgatgc cacttcccac tcgaggcctt cagcgcctgc
     2881 gccgtccgga tcgttccatg gacgctgggg agctttttct cccttgccgc gcatggtggg
     2941 caggaccacg aagaagaaga aatagacctg ggcaaagccg aagaagaagg cacccacgct
     3001 ggcaatcgcg ttgaagtctg caaactgcat cgggtagtcg gcgtaacggc gcggcatgcc
     3061 ggccagaccc aggaagtgca tcgggaagaa ggtcacgttg aaggaaatca gcgaccacca
     3121 gaagtggatc ttgccgcgct tctcgttgta catcacgccg gtccacttgg gcgaccagta
     3181 gtagtagccg gcaaacagcg catacaaaga gcctgccacg agcacgtagt ggaagtgggc
     3241 caccacgtaa taggtgtcct gcacctggat gtcaatcggc gcgaccgcca ggatcaggcc
     3301 ggtgaagccg cccatggtga acacgaagat gaagccgacg gcaaacagca tcggtgtttc
     3361 gaaggtcatg gagcccttcc acatcgtcgc gatccagttg aagatcttca cgccggtcgg
     3421 cacggcaatc agcatggtcg agtacatgaa gaagagctgg ccggtcaccg gcatgccggt
     3481 ggtgaacatg tggtgcgccc acacgacaaa gctcaggatg gcgatggagc cggtggcgta
     3541 caccatggag gcgtagccga acaggcgctt gcgggcaaag gccgggatga tctggctgat
     3601 gatgccgaag gccggcagga tcatgatgta gacctcg
//
LOCUS       DAIDFG010000310         3585 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_310_length_3626_cov_20.255391, whole genome shotgun sequence.
ACCESSION   DAIDFG010000310 DAIDFG010000000
VERSION     DAIDFG010000310.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_310_length_3626_cov_20.255391"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          576 a         1211 c         1231 g          567 t
ORIGIN      
        1 aaagcccgct gcgccagcag ataaaagcgc aggctcaggc cgtagccggt ggcgccgacc
       61 gccagcagcc cggcggcagc cgccagagcg ggcgccggtt cggcgaaaac cagcgcgagc
      121 agggccgtga cggtggcgcc cagcgccgcc ttgaccaaca ccacctgtcc cgggtcgcgc
      181 tcggcgagcg cacgtgacag ggtgttgtcc atgccccagg cggccgtggc caccagcacg
      241 gccagcaggc cggcgagctg ggccccgccg agccgggcct ggtccagaac cagcacggcc
      301 ccgccggcca gcaacagcgc catggcgacc cagacgcgca ggcccagggt ctcacggtaa
      361 agccgccagg ccagcagcgc cgtgaacagc gcctccaggg tcagcatgag ggatgcactg
      421 gcgccgctgg tgcgctgcag gccccaggcc agcgccaccg gcccgatcac ggcgccgaag
      481 gccaccaccg ccagcaaacg ccgcccgtcg ctgcgctgca gactggcctc ctgctggcgg
      541 cctttcctga gcagcgcgcc ggccagcgcg gcaccggcgt acaacagggc tgccgtggtc
      601 agcggcccca ggccccggcc caggtactgc accagcggcg tgctgacacc gaaaagtgtc
      661 gccgccagca acgccagcag gccgccgcgc aaagcgggtg tgttcatgcc cggatcctca
      721 agagatcaat caaatcagcc tccagcccaa taccaacaag cgtcaacagc tatctttttt
      781 gcagcaagcg gaccagacgg ttgctggtgt tgcgcagccg ctgggccagc agctgggtga
      841 tcttgaccat cagcttggcg ccgacgccgg ggtgctggcg tatcagctgc ccgatggcgg
      901 cgcggctcag caccgcggcc tccaccgcgt cgatggcggt gcaggtggcg taacgcacgt
      961 cgcggtcgaa catcgacatt tcgccgagga ctgcgccctc ggtcgtcacg ccgaggcggg
     1021 acttgttgtc cgcggtgtcc gggtcgtcgt cgttcgccag cgtggttttg gtcacatcga
     1081 ccgtgccgct gagcagcagc agcatccagt cgccggcatc cccctcggcg atcagcacct
     1141 gcccgggctg cgcgcggatc agcagcatgg cgtcaccgag cgtgtcgagc tccttcatgg
     1201 tgaagtcttc cagcagggcc gagtgctgca gcagccccgg gccgcggcct gacatcttct
     1261 ggcagctgcc cagcacttcc agcccggcct gccgcaactg gtcttccagg gaagccggtg
     1321 cccccgtgcc cgccgcttcg ggtgccggcg gcgacgcggc ggcgttcagg ggatctgctg
     1381 cagccataac gagatctcct cgatcgcagc gtcggtcgcc gcggtcagcg cccgcacgcc
     1441 gccggccgcg tcggcgctgg tggccggccg ctggaccgtg aagtagcgct gggccagcag
     1501 tttttcgccg ctcggcgtgt tctccaccaa cgtggcgtgc agccgcacca ggccggtgct
     1561 gctggcgggc gactggaaca ggtggctgaa ctcctcgagc tgcagctgca gcgtgcgcgg
     1621 cggcggcccc agagcggcgc ttcggttcag ggccggacct tccccggcgt tgaagacggc
     1681 ccggtcgcgg cccagcgtct cgcgcaggcg ctggcgcacc agctgcgcag gcggcatgct
     1741 ccagcgtgcc tgcgagtagg gccgcaactg ctgcgcatcg gcataggcca gccggtacag
     1801 caccgcctgg ttgtcgagta cgccgccggg cgttgaaatg tcgctaagtg ccagcgccgg
     1861 cagcggcgcc tgccgggttg cgggcggcgt gttcaggagg ccgggcccga agtcgtagag
     1921 cgcggcgcgc agcggcttgt cgggcagggc cgagcaggcg gcgagtgtca gcgccaccag
     1981 aaaaatcaag ccaaatcggg ctccagcctt tttctggcgg gcgccggcag ctacggtatt
     2041 gatagcattt acgggattca tggttttcct ccgggagcgg taaagcccgc ctcacccgga
     2101 ccgggcgtgg gcgggccgtt gccgaaaatc agcgattgcg ggttgtcgtc caccgcgttg
     2161 acggtgcgcc ggagctgacg catggcgcgg gcggtttcct cggccacttc acccagcctt
     2221 ggcagggtgg ccgcgctgaa ggtctccacc gtggccgtca gggcggcacc gccttcggcc
     2281 agcttgtcga ggggcccgcc cttggcgttg aggcgcccgg ccgtggtgtt cacttcgttg
     2341 gcggtcctgg acacatcatc ggccgcggtc ttgagcgact tcatggtgac gctggcctcc
     2401 tggctcagcc tgggcagtga agacaccacc gggtccagct tcagggccac cttgttgatg
     2461 ctgtcggccg cctggccgaa ctgctggaca gcgctcatca gcgcctgctg gttgctgtcg
     2521 ccgagcagct tgttgagttt ggtgctggcc gcctcgacct ggtccatgat cacttcgctt
     2581 ttgtccagca gcttttgcag caggccgggc ttgagcggga tgcgcggcgg atcgtcgtcg
     2641 ttgggcacca gcagctctgt agaaggaccg tcgtcttcga gctggataaa gcccagcccg
     2701 gtgacgccct gcgagctcac ggtagcaaac gtcgattggg tcaggggcgt gccgacatcg
     2761 accgaaatgc gcaccagcac attgcccttg gtcttgtggt caaagccgat gaactcgacc
     2821 ttgccgaccg gcacgccgcg aaaacgcacc tgcgcctgcg gctgcaggcc cgagagcgtc
     2881 tcacgcgtgg acagctcgta caggtggcgc tcgccgttgt cgcgtgtcag ccagacggcc
     2941 agcagcgcca gcagcgcagc caccaccagc acaaaagcgc cggcggcgat ggcatgggct
     3001 ttgttttcca tttttgaagt ccttactatc tctgtgaggt ggttccagaa attcgttgat
     3061 cgggatgcag tgcaaggcgc ccggagcgca ggacaccgga atgcacttcg gtgcatgagg
     3121 attccgagca ccgcgcaacg cagcaatgcg cccgaactgc ggatttatgg aggcacctca
     3181 tacggtgaag gggtattcgc gcagcagctc catcgcgcgc tggccccgtt cgccgagaaa
     3241 aaatcggcgg acaaacgggt ggggaaaggc aatcacgtcc cggggcgggc cgctgacaat
     3301 gacctgcctg tcggccagca ccgaaatgcg cgtgctgagc tcgaacagcg tgtccaggtc
     3361 gtgcgtgacc atcaccacgg tgagctccat gtcgcggtgc agcgagcgca gcagggcgca
     3421 gaagctgtcg gaggcttccg ggtccagacc ggcggtcggt tcgtcgagca gcagcagcgg
     3481 cgggtccatg atcagggcgc gcgccagcga cacccgcttg accatgccgc cggacaggtc
     3541 ggcgggcatc ttgtcggcat gttcgggcgc caggccaacc atctg
//
LOCUS       DAIDFG010000311         3619 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_311_length_3619_cov_19.715208, whole genome shotgun sequence.
ACCESSION   DAIDFG010000311 DAIDFG010000000
VERSION     DAIDFG010000311.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_311_length_3619_cov_19.715208"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          548 a         1174 c         1200 g          697 t
ORIGIN      
        1 caacccgaaa atatcgaaac atgaaagcgc gccttcgcac ccatggcggc gttgccatcc
       61 ttgcgaaggc gtacagcctt cgctgccgct ggcgccttgc cctgggcacg aagccatcac
      121 tttcattttg tttcgctact tccgtgctgc agtactagcg ccaaaacccg gctgcccatt
      181 tttctagcag gagatttcgc catggcctat caagatttca atatggacca ccagtggttg
      241 ccgttcacgc cgaaccgcag cttcaagaaa gacccgcggg tttttgtgaa agccgacggc
      301 atgtttttca cgacacacga cggcaagcag gtgctcgacg gcatctccag cctgtggtgc
      361 gtgggcgccg gtcataaccg caagccgatc aacgaagcca tcaagaagca gctcgacacg
      421 ctggactacg cgaccgcctt ccaggccagc aacgaccagg ccttcaaggc cgccgacctg
      481 attgccggca tggcgccggg cgatctgaac caggtgctgt tctgcaattc cggttcggaa
      541 gcggccgaca cctcgctcaa ggtggcgctg gcctaccacc gcgcacgcgg cgagggccac
      601 cgcaatgtct tcatcggccg cgaaaagggt tatcacggcg tcggtttcgg cggcatgagc
      661 gtgggcggca ttccggccaa ccgcaaggta tacggctcgg ccttgctgcc ccgggtggac
      721 cacatgcgct tcatccacga cccggtcaaa cacgcctaca tccatggcgc cgagccggtc
      781 tgggaagaag acatcctgct ggagctggag cagcgcattt tgccgctgca cgacgcgagc
      841 aacatcgccg cggtgattgt cgagccggtg gccggcagcg ccggctggta cctgccgccc
      901 aagggctacc tcaagcgcct gcgcgagatc tgtgacaaac acggcatcct gctgattttt
      961 gacgaagtca tcaccggctt cggccgcatg ggcacgaatt tcggcgccga ctattacggc
     1021 gtggtgcccg acatgctgaa cttcgccaag tgcgtgacca acggcgtgat tccattgggc
     1081 ggcgtgatct gcaccgaccg catctacaac accatgatgg aagccaatgc cgacagcccg
     1141 gcgcatgcgg tggagttttt ccacggctac acctactcgg gccacccggt ggcctgcgcc
     1201 gcggccattg ccacgctggg cctgttcaag gaagaaaagc tgtttgaacg cgccggacag
     1261 atgggcaccg tgcttggcga cgccatgcac agcggcttca agggtctgcc caatgtggtc
     1321 ggcatccgca gcctgggcct ggccggtgcg gtggagctgg cgtccatcgc cggtgcgccg
     1381 ggcaaacgcg cctacgacat cttcatggat tgcttcaaac acggcgtgct ggtgcgcccg
     1441 gcggccgaca acatcgtgat ctgcccaccc tacatcgtgg aaaaagagca catcgagcgc
     1501 atcgtcagtg tggtggcgga cgccatcaaa aagaacgcct gagggcgcga taggggccgg
     1561 ggctatttcg ccccgggctt ctccttcaaa cttgcgcagt agtcctgctc cggcacggcc
     1621 ggggtgggcc acacggcgtc aaacgctgcc ctgttttcgg cctcctggcg cgcatccggc
     1681 gcgtttgttg ccggaccggc gcgtagttgc acagccttgg catggaggcc gggcggcaga
     1741 tgctggggca cacccagcgt gcggttgacg tggccgctgc cggccagcaa caccactgtc
     1801 tttccgggca gggccgcctg ctgcagcgtg cgcgccatgc ggatgtcgcg cgcgatctgg
     1861 acgcgtgtca tcggggagat gcggtcctcc ggcaacaagc cgcagtggcc gatgcgtatc
     1921 agctgctgct gggccttgag cgcgggcccg ggcaggggct ggtccagtcg gctgtcggcc
     1981 atggcttctc gtacctgcgc tgccggcagg ttggcgccca gcaccggcac gccggccttg
     2041 acggccgcca tcaccgccgg gccgtaggcc gcccagggcc agctcttgtt gttccagccc
     2101 agggcggcct gcacctgcag ctgggtgctg ttcgatttca gggccgcggt gctggtgccg
     2161 gcttctgcca tctccaatgc cacggcggcc agccggccgc gcaccgccag gtactcgatg
     2221 acctgccgct gaatgtcctg gtggtcggat gcatcgtgct gttcgccgag caggatcacg
     2281 tcggcaggca gcagggcctc ggtgcgtgcc gtgatgtcat tgggcgcatg cggaaacagc
     2341 cggggttctg gcactctggc gcaggcggcc agcagcatga tcagcagcgg gaccaggacg
     2401 gcaaggcccc ggcgcggttt cgggaaggtg gggatggtgg tgttcatggt gttggtcaag
     2461 gcgatactag ccccatctga aaaccggcgg ttgcctgccg gcccatgccc cgcacctctt
     2521 gcctcttgct tttttttttt cgggatgcgt ctcaagccct ttcatgttgt tttcaccctg
     2581 ttgctggcgc tggctggcgc atggctttgt gtctggatcg gcacgcccct gccgtggatg
     2641 attggcccgc tgctggccac ggcctgcgcc tcgatcgccg gcacgccgac tgccagctgg
     2701 cggccgctga acaacaccgg ccaatgggtg atcggtacgg cgctgggcct ctatttcacg
     2761 ccgcaggtga ccgcgctggt gggcagcctg tggtgggcca tcgccctggc gattgcctgg
     2821 gcgctggggc tgggcctggc cttcggcggc tggttgcacc gtgtcaatgc accgcgcatg
     2881 cccggcgtgc cggcgccggc gatgcgtgcc accagttatt tcgcgggtgc cataggcggc
     2941 gcttccgaga tgaccctgct ggctgaacgt gcccatgcgc gcaccgacct ggtggcggcc
     3001 tcgcacagcc tgcggctgct gctggtcacg gtgagcattc cttttgcgat gcagttcagc
     3061 ggcctgcatg gcctggatct gaacccgccg gtgctgcgcg cggtgaacgt gcctggcctg
     3121 gccgcgctgg ccttggccac cgccgcgggt gcctggttga tgctgcggct gggccgggcc
     3181 aacccgtggt tcatggggcc gctgctggtg accatgggcc tgacgatggc cagcctgcat
     3241 ctgtcgggca ttccgccctg gttgaccaac accgcgcaac tgttgattgc ggtggggctg
     3301 ggcgtgcggt tttcaccgac ctttctgcac gcggcgccgc gctggatggc ctcggtggtg
     3361 ctgggaaccc tgggcatgtt ggtgctctgc agcggcttcg ccgcactgct ggcatgggtc
     3421 accggcctgc cgctggccac gctgattctg gggacctccc cgggcggcat cgccgaaatg
     3481 gccatcaccg ccaaggtgct gcagctcggt gtgccggtgg tcacggcgtt tcaggtgtgc
     3541 cggctcgtgg cggtgctcat tctggtggcg ccgctgtacc gctggctgta tccggcggac
     3601 tgagtccgcg tcttccatt
//
LOCUS       DAIDFG010000312         3616 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_312_length_3616_cov_16.878405, whole genome shotgun sequence.
ACCESSION   DAIDFG010000312 DAIDFG010000000
VERSION     DAIDFG010000312.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_312_length_3616_cov_16.878405"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          640 a         1194 c         1151 g          631 t
ORIGIN      
        1 tgttccacag ctcgacctcg cccagtgcgg tctcgtagac cacgcgcagc cgtgtggcca
       61 gggcccgcac cagccgttcg gaaaaatccg gctgcgccag cttgaggatg ttggccacgc
      121 ccaagtactg caggtggctg cgttcgatct gctccaggtc ctccaggaaa cggttcattt
      181 caggctcttt gggcgcctgc agggaaaaac cgaactcggc attgagctgc ttgaacgatc
      241 cgcccagcat gctctggatg tcagcgctgg tggtctgggc tttcttcagg ccttcgcgca
      301 gccgcccaaa ggtctggcca taggctttct tgacaccgag cttgatgccg ggctgcttga
      361 gcgcgtcggt cagttcggcc agctccgtct tgagggtcgg cgtgcccagc aggtggaaca
      421 cttccctgag cagcttcaga tggatggaac gcacggcctg tatcctggcg ccgctggcgt
      481 tgaattcgac ctgctcctgc tcgatgcgcg agcgcatgtg tttgatgacc gagtcgttct
      541 tgccgcgcag gccgcgcagt tcgatcatct gctcggccag gtcgcggcgg cggatgtgga
      601 tgacgcggcc ggcctcagca cgcaattcga caatgccggc ggccaccgca gagcgcagta
      661 ttttctgccg ctgccccatc acgccctgcg ccagagccag ctccagcccg ggcaggcagc
      721 tggcctgcag cagcttgtca tcgcctgtga ctttggcgac caggcctttc tgggccgaga
      781 cgggaatgac ctgcgccacc ggcagcccga gaatttctgc cgagcttgca cgctgacggt
      841 cgatctgggc ctggatctgc gcgggcgagc tcagggcgtc ccacagggtg tctatcttgt
      901 tgagtaccac caggcgggcg tcgttgtcgt caccctcggt gatcaggtgt tcacgccaga
      961 tggccaggtc ggatttggtc acgccggtgt cggccgccag gatgaagacc accgcgtggg
     1021 cctgcggaat caggctcacc gtgagttccg gctcggcgcc aatcgcgttg agccccgggg
     1081 tgtccagaat caccagcccc tgcttgagca gcgggtgggc gatattgatc agcgcatggc
     1141 gccacttggg cacttcgacc agaccgtcgg cattgacccg cggattgtcc tgcggcgcgt
     1201 cgtcatgcca gaaacccagc gcgcgcgcct cgtcctgcgt gacgtggcgg acttcggcca
     1261 ccttttccag cgctttcgag agcttgaccg gatcgttcac atcgaggtcg acacgtgtcc
     1321 atttctcggg aaccatgcgc cactccatca gggcctgcgg cttgagccgg gtctcgatgg
     1381 gcagcaggcg caggcagggc ggtgagtccg cgtcgtagcc cagttcggtc gggcacatgg
     1441 tggtgcggcc ggcactggcc ggcatgatgc gacggccata accggcgaaa aagatcgcat
     1501 tgatcagctc tgatttgccg cgcgaaaatt cggcgacaaa agccaccatg accttgtccg
     1561 aacgcacctg cgactccagc cggagcaggc gttcttccac ggcggcgttg agcaggtcgt
     1621 ggcccttcat ccattcggat aaaagcttga gacgcaaggc aaactcgcgc cgccaggcgc
     1681 cgtgctggtc aaattgttgg ttaaaggaca tggtgcgccg tggacatagg tcaatcaata
     1741 tagcatcgcc caccggaaac ggtcagcccc cgggcagacg gatgttcagg gtttctggca
     1801 gttgacacag taaaaagtgg aacgttgccc ctgccgcagc atgcggatcg gcgccgcaca
     1861 cagcttgcag ggaaggccgg cccggtcgta aaccatggct tcgagctgga agtagccgct
     1921 ctcgcccatg gcgttggaaa aatccttcag ggtgctgccg ccttttgtaa gcgcctgtgt
     1981 caacacgctg cgaattgccg catgcagcct ggcggcgcgg ggtttgctca gccgccccgc
     2041 ccgcacggtc ggacggatgc cggccatgaa cagcgcttcc gaggcataga tgttgccaac
     2101 tccgaccacg atctcgccgg ccagcaacac ctgcttgatc gccgtctgcc tgccttgcag
     2161 cgcacggtaa aaggccagcg cctcaaaggc gtccgtcagc ggctccacac ccagccggcc
     2221 cagaagcttc tgcgcagcag cgtcgtgctc ggcaggcgcg tacaccacgg caccaaagcg
     2281 gcgcggatcg tgcagtcgca aggtgcctgc cgtggtacgc aggtcgaaat ggtcgtgttt
     2341 cccgggtggc ggcaagtctg cagcgaaacc caggctgccg gacatgccca gatggatcag
     2401 cagcaggccg ccggtggtgt cgagcagcag gtatttgccg cgcctgcgga cctcgagcac
     2461 ggtgtgcccg gtcaggtgcg cggcatcgca gcccaggggc cagcgcagcg gcttgcccat
     2521 ctgcaggtcc agtatttttg cgccggcaat gcgactggca aaactcaggc gggtgacttc
     2581 gacttcaggc aattcgggca tgggcaactc ggggcaatcg ggaaacacgc ctacatgggc
     2641 gcagggcttc aattatcatg gtccgatgaa acaacttatc agcgttttca gtgcactttg
     2701 cctggcagtt ccggtgctgt gggcccagac cgcccagcct gccccggcct tgccacccgc
     2761 tgcccagcct gaggcgccgg ccgcgaccca gaccctcacc cccgtacctt ccagcctgga
     2821 cggagagctg ttctaccagg tgctgctcgg tgaactcacg gcccgcgaag gggatgcccc
     2881 gagcggtttt tcactgatgc tggacgctgc ccgcaaaacc aacgacgccc agctgtacca
     2941 gcgcgcagcc gacattgccc tgcaatcgcg ctcgggcgac gcagcgctgc aggcggcgca
     3001 ggcctggcgc caggcccagc cggcctcgcg ggaagccaac cgttatgtgc tgcaaatcct
     3061 gctggcgctg aaccgcatca ccgagaccac ggagccgctg aaaaatgagg tgaagctcgc
     3121 gccccaggtc gaacgcccgc aggccatcac cagcctgcca cgattttttg cccgtgtcgg
     3181 cgacaaaaaa ctcgccgcca gcgtgcttga gcaggcactg gccgacgagc tcaaggcccc
     3241 cgccacgggc gcagccgcct ggaccgccgt cgggcgcatg cggctggccg ccggcgaaca
     3301 gcccggcgcg ctggaggcgg ccgaacgcgg gcaggccatc gaccccgcgg ctgaagggcc
     3361 gaccctgctg gcactggaac tgatggaccc caaactgccg cgcgccgagg ccctggtcca
     3421 gaaatacctc gagggcaacc ccaaggccgt gcctgaactc cgcatggcct atgcccgcgt
     3481 cctgctcgat gtccagcgtt atgccgaagc cagcacgcag ctgcagctgg tcacgcgcga
     3541 caagcccgac taccccgaag gctggctggt gctgggctcc ctgcaattgc aggacaacca
     3601 gctggcggcg gcgcag
//
LOCUS       DAIDFG010000313         3613 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_313_length_3613_cov_19.481169, whole genome shotgun sequence.
ACCESSION   DAIDFG010000313 DAIDFG010000000
VERSION     DAIDFG010000313.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_313_length_3613_cov_19.481169"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          649 a         1111 c         1157 g          696 t
ORIGIN      
        1 ggagaccgag taggagaatg cctcgagttc cgcattcgtc agccgcaagg cggtctcggt
       61 cgccttgatt tccgtgatgt ccacatcaat gccgacccag aacatctcct ctccggcgtc
      121 gttgagcact ggtgacacgc ggtaggacat gaggcggtag ctgccatcgg gcgtgcgcag
      181 gcgccgtgtt ccggcgtggg gtgtgcgtgt ctggcggtgg ccatgccatg cttcaagcat
      241 gccgggcaaa tcgtccggat gcaccagccc catccaggcg gtgccgtgcc agtcgggggg
      301 tgcgccgccg aaaagttcgt accaggcctg gctgacaaag gtggcattgc cctccgggtc
      361 aatcgtccag atcgggtacg gtgcctgctc ggccagcgcc ctgagcaggg cttcctggcg
      421 ggacaactcg cttgtgcgct gggcaatttt ttcctccagt ccgctgttga actcctgaac
      481 ctgtgccagc aggttgacgt tgtcgatcgc gatcagggcc agctcggcga gttcggcaac
      541 aacgtattcg tcctgccggg tgaagtcgcc ctcgtatttg tccgacagct gcaacaggcc
      601 gacgttgctg ccgtcccgtg ccgtgagcgg cacggcgagc cagccgttca tggccgggtg
      661 cttgtctgcg tagcttccca agccgcgcca gcgcggatga gctacaagct cggcctgggt
      721 caggcgcaag ggccggttgg tttcgcaaac gaccgcatag ataccgctgc cgttgggcgg
      781 ttcgatcaaa tgccgctgcc tggcgtattt atcggacagc gacagcgccg tgatgccctg
      841 ggaccagtcg ccgccttggg tcaggctgac aacggcctga tgcgcgccga tgatgctgcg
      901 cgcctgctcg gccacttcct gcatgaccgc ctccagcgtg tggtgctggg tgatggcctg
      961 tgccgcctct gcggcacgct gcaaggtctg cagatgggcc tggacttcat gctcggcctt
     1021 gacgcgttcg gtgacatcct gcagcgcccc ctgcatgcgg atgatgctgc catggccgtc
     1081 ccgcacggcc tggcccagcg agcgtaccca gatgtggcgg ccggtgccgg tcaccatctc
     1141 cacctcgacg tcgaagggcg tgccgctttc cacacattcc cgtgctaccc gggtggccag
     1201 cgcctgcgac tgttctgtgc aaaggcggat cgcggaggcc agggtgggcg cgctgtccgg
     1261 tggctgctcg aggatcaaca aggcctcatc cgaccagaga aagtgcatct cgggcaactc
     1321 cagtgcccag ccgcccaccc gggacaactg ccccgccatg cgcagcagct cggtttgggc
     1381 atgcagcgca gcttcggtct gtttcaggtc gtcaatgtcg gtggaagtgc cgtaccacga
     1441 gacaatgcgc ccggcgtcgt cgcggttggg aatcacccgc gccagctgcc agcgataggt
     1501 gccgtcaccg cagagcaggc gatgttcgtt gtgcaggctt tgcccgcttt cgatggcaag
     1561 gcggaaatcc tcggcggtcc tttccagatc ctccgggtga acacgtgtga accagccgcc
     1621 ctggagtgaa gcctccgccg gcaggcccgt cacgtgggtc cattgttcat tgacgaacgc
     1681 catgtgcccg cttgcatcgg tgatgaacac gatttgcggc atggcctggg ccagctggcg
     1741 aaagcgcaac cgggtgcgtg cacgctgctg ggaataccgg tagagcagcg ctgacagcag
     1801 cgccactgtc gctgcggctg ccagccagat cagccaggtc agcgtggcaa aacgcttcca
     1861 gggcgcgagt acctcgtcgt acggggtgcc aacgtagatc accagctcgg gataggggtc
     1921 gatgacccgg taggcgatca tgcgggcacg gccatcgatc acgctggtgg tctggtaaac
     1981 gccttgtgtg cttttgggga gctgttcgga aaacaggggc aggtctggaa aggccttgcc
     2041 catggcggtg tcgtccatcg ggctgcgcat catcagcatc ccgtcccggc ggaacagcgc
     2101 aatcgcaccg ccgccgccca ggtccactcc gcgccagagc ctgtcgaaat aaggcggttc
     2161 cacggcggcc acgatcacgc ccgcgaagct gccgttctcc gcccgcaggg gcctggacgc
     2221 actgatcagc caactgcccg tggagcggct gcgtaccggc gtgctgatgt ggaaaccggc
     2281 agacgggcgc tgctggtaga tctggaaata ctcgcggtct gtcagcttgg ccccgatgtt
     2341 gccgaggtcc gagtcgaaaa cgatgcggcc gccggcatcc agcacccaga tggcgcgcac
     2401 gaaggggagg ctgctcagct gctcgcgcag catggtccgg gccgtgtgtt cgtcaagccg
     2461 cttttcatct tgcaatacca gcagccggct ggccgcaagt tgaaggcgct ggtccaccgc
     2521 ctgaaaggac cggccggttt gttcctcgat cacctgtacc agcgactggg tcagtttctc
     2581 accgccgtgc aaggcttcgc cgcgaagata aaagagggcg acggccaccg cacaggcgga
     2641 aaccaccatc aggccgccac tcagccaggc gatcgggcga ccgatcgaat cctgcgctga
     2701 gtccgcatgc gtttgtgatg gccggagcgg cgcctcaact tgccagggcc aacgcatgca
     2761 tccagccttt cagggggggg ccggtcatgt tgcccggcgt gaggccatga tgccatgtgg
     2821 gtcgggcttt gtcgattgcg acgggcccct ggtacttcgg tctcgaagta cggctgcatg
     2881 aaagcgaaag cgcgcctgtt gtcgcagggc gtccccgcga gaagcgttgc tggatgtctg
     2941 gtaggccaga gaactgggtt ctgcgccctg atggatcaga gggaagttaa tgcgtagaat
     3001 gcgtgccgga gcacctctgc cggggtggct cacgcatcca aatcaaaact atctgaggga
     3061 gctacacatg aaactacaag tactgcttgc tgtcaccgcc atatctttga tcgcctgtgc
     3121 aaatcgtccc gattcaatca gggcttcgca cgtgtcctat gagaaattca ctcatctcga
     3181 ctgcgccgcg ctgaccatca aaatggcttc aacgcgtgca gaccttgaca agttttccaa
     3241 aatgcaggac accaaggcaa acggtgatgc ttttggtgta ttcctcttgg gcatcccttt
     3301 cagcaaactg tccggcgatc acgaaggtga tgttgcaaga ctcaagggtg aggtcgaagc
     3361 tatagaaact gcccaggtaa aagggcagtg cacggctgag tcgaagacga caaaatcaag
     3421 ctgacggctg tctggaaata gtcagcgcgg gctagacgcc caggtattgc gtcagcagct
     3481 ccggccgtgc atgcaggtcg gccgagctgc cttcgaacac cacctcgccc ttgaccagaa
     3541 tcacgttgcg gtcggtgatc cgggtgacgt gtttccagtt cttgtcgacg atcacgctgc
     3601 tgatgccgct ctc
//
LOCUS       DAIDFG010000314         3604 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_314_length_3604_cov_19.160327, whole genome shotgun sequence.
ACCESSION   DAIDFG010000314 DAIDFG010000000
VERSION     DAIDFG010000314.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_314_length_3604_cov_19.160327"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          556 a         1189 c         1187 g          672 t
ORIGIN      
        1 agtaaaagct gggacgagtt tggccagccg gattcacgcg cgcgccgcgc cgggccgccc
       61 caagcaagcg cagccccctc ggggggcagc gcattacacg aagtgaaaag cgtgggggcc
      121 gtacctttga tggatctggt gatcacggtc tgcgacaacg ctgccggtga ggtctgcccg
      181 tactggcccg gccagccggc caccgcgcac tggggttacg ccgatccctc ggacgtcgag
      241 ggtgatgagg cgaaaaaact cgaagccttc cgtcagaccc tgttcgcgat acgccgccgc
      301 ctggagctcc tgatcaacct gccgcccgaa aaactcgcgc gggccatggt gcagcaagcc
      361 gcgcgggacc tggccaagca ggcctgagca aagccattca caaaaaaaag gaagtgtcat
      421 gggattgttt gaacgttacc tcaccgtctg ggtggcactg ggcatcgccg ccggcgtggg
      481 cctgggcctg ctggcaccaa ccgcctttca gggcattgcc gcgctggaga tcgcccacgt
      541 caacctggtg gttgccgtgt tcatctgggt gatgatttac ccgatgatga tccagatcga
      601 ctggtcggcc gtgaaagacg tgggcaagaa accgcgcggc ctgatcctca cgctggtggt
      661 caactggctg atcaagccct tcaccatggc cgcgctgggt gtgctgttct tccagtacat
      721 ttttgcgccc tgggtcgatc cgcaatcggc cagcgaatac atcgccggca tgattctgct
      781 cggtgtggcg ccctgtacgg ccatggtgtt tgtctggagc cagctcgtca agggcgacgc
      841 caactacacg ctggtgcagg tctcggtcaa cgacctgatc atggtggtgg cttttgcgcc
      901 catcgccgcc ttcctgctcg gcgtgaccaa catcaccgta ccctgggaaa cgctggtgct
      961 ttccaccgtg ctgtacgtgg tgctgccgct ggcggccggc atggcgacgc ggcatctgct
     1021 gcagcgccgc tccagccacg cggtggccga cttcgtggcc aagctcaagc cctggtcggt
     1081 gatcgggctg attgccaccg tggtgctgct gttcggcttc caggccgaaa cgattgtggc
     1141 caaaccgctg gtgatcgcca tgatcgcggt gccgctgatc gtgcagagct acggcatctt
     1201 catcatcagc tacatcgcgg ccaaagcctt gagcctgccg cacaacgtgg ccggcccggc
     1261 ctgcctgatc ggcacctcca acttcttcga gctggcggtc gccgtggcga tttcgctgtt
     1321 cggcctgcaa tcgggcgcgg cactggccac ggtggtcggc gtgctggtcg aagtgccggt
     1381 gatgctgtcg ctggtggcca tcatcaaccg cacgcagcac tggtttgcac cgcagcgcgc
     1441 ttgaggggct ggccggcggc ggcgttgctg ctgcatggcc atttcttcat atcaaatcgg
     1501 cctccagccc tttatccacc tgcggcaaca gctctgtttt tgatagcacg caaaccctcg
     1561 ccacgccatg agccaacccc gcagcgtcac cgtcgccagc ctcggcatcg cccagaccct
     1621 ggcgtgggcg tcgagctact acctgccggc catgctcgcg aactcgatgg cgcgcgacct
     1681 cggtgtcagc acgccgacgg tattcctcgg gttctccgta gcgctggtgg tgtccgccct
     1741 gctcggcccc tttgccggca aggcgattga ccgttatggc ggccggccgg tgctggccgc
     1801 caccagcttc atttttgccg gcgggctggc ggccctggcc ctggcgcagg gaccggtcgg
     1861 cctgttcgtg gcctgggcca ttctgggtgt ggccatggcc gccggcctgt atgaagccgc
     1921 ctttgccacg ctggtgcacc tgtacggcaa ggacgcgcgc ggcgccatca ccggcatcac
     1981 gctactggcc ggttttgcca gcaccgtggg ctggccgctg tcggcctggc tggaactcga
     2041 atggggctgg cgtgccgcct gcggggtgtg ggccggcctg cacctgctgg tggcgctgcc
     2101 gctgaacctg atgctgcctt ccacaccggt agcgccagct gagccgccgg ccgtacaggc
     2161 catgccagca gaaccggcgc cggccggcac cctctccgca tctgaaaccg cgtccgtaca
     2221 aaacccgacg cgcatggtgg cgctgctggc ctttgtgttt gccgcgacct ggttcatcag
     2281 caccgccatg gccgcacacc tgccgcgcct gctgcaggtg cacggcgtgc cgctggccac
     2341 cgcgctggtg tttgccgggc tggtcggccc ggcgcaggtg gccgggcggc tgctcgagta
     2401 cgggctgctg cgcaacatcc atccgctgat ctcggcgcgc gtcgccggcg ccatgcacac
     2461 gctgggcgcc ctggtgttcc tggctgtcgg tgtgccggcg ggggcggtgt tcaccgtgct
     2521 gcacggtgcc ggcaacggcg tgctgacgat tgccaagggc acgctgccgc tggtgctgtt
     2581 cggcgccaag aactatggcg cgcgccaggg cctgctgatg gtgccggcgc gttttgcgca
     2641 agctctctcg ccctggctgt tcggcctggc gattgaccgc ttcggcgcgg gcgccttgtg
     2701 gttttcagcg gcgctgggcg cgctggcttt tggggccttg atgctgctgc agggcccgcg
     2761 cggggcaccg gtccggcccg cctgattcag cgcgccctca ggccagccac ttctgcagaa
     2821 actgcgcctg acccatggcc gggtcgagct ggtaggcggc aaaaccgatg cgttcataca
     2881 gacggatggc ggacgcattg ccctgcagca cttccagcgt gagtttgcag gcgccacgct
     2941 cgcgcgcgat ggcctcgacc tcggccagca tcagctcacc gacacgccgg ccgcggtggc
     3001 tggccagcac caccacgtcg tgcacattga ccagcgggcg gcaggcaaac gttgaaaacc
     3061 cctcgatgca gttgaccagc ccgaccggct gcgtgccgtc gaaggccagc acgctgaagg
     3121 cctggggccg cgcggcaagt tccgccacca ggtgggttct ggcgaagtcg ctcaggggct
     3181 gcccgccgcc ggccttgtca cgtgcgtagg cgtcgagcag ggctaccagc gcgtcggcgt
     3241 gtgcggggtt gcggtagtca gcgcggctga tgcgcaggcc ggaagcggcg atagctgtca
     3301 tgcggggagc cctgtggaaa ggtggatgtg gtgattttaa gcagccaacc cacgagtccg
     3361 gcgcagtacc acttctggaa tcaccaataa aaatggtcgc cagcccagac aggtaaagcg
     3421 ccggcagcta tcaaaaacag agcaatgtac gctggcagac agttgccccc agcactttcc
     3481 gccatggtga atgctgggcc gccgctcttt accggacatt tacgaagccc tttccggcgc
     3541 tacacctgag cgcgaagcct tgcagtgata attcttcagc gagctgaaaa atacacgctg
     3601 gcag
//
LOCUS       DAIDFG010000315         3597 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_315_length_3597_cov_15.937041, whole genome shotgun sequence.
ACCESSION   DAIDFG010000315 DAIDFG010000000
VERSION     DAIDFG010000315.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_315_length_3597_cov_15.937041"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          708 a         1068 c          972 g          849 t
ORIGIN      
        1 cgctttccat ccacacatgc gcccgaacaa ggcggttata gaaggtttta tctggcgaaa
       61 acgggtagcc ctcacccatc agggccatga tggtccacgg catgtagtgc atcttgattg
      121 cggcggctaa ttgcagatcg gcttcgcaca ggctggcaaa ggctgcgtcc gtgtcgctgt
      181 ggtcctctgc ggcgtagccc gtggggtcga ttgagctggc cggcgattga atgtaggcaa
      241 agtccaggct tttgcgcggg tagcccagcc cgccgcttac agacttcatc tggtggatta
      301 cccagcggtc aagcagggcg caaaggtcgg tgtgtgattc aaggattttt agtctgcgtg
      361 ccatgtttcc gtccttttag ttgttaaccg attccgcagc cttgattgcc gcctgcctgc
      421 gggcgaagta ctgaatcaca cccagcgtca ccccgaacct ctgtgctatc tcgcgctggc
      481 tgaccttttg ggccatcagc accagaacgc gcctttcgtc tagctgggtg cggggctttc
      541 ctgcccctgc tcgtttgccg ccgtggttca ttggctgcgc cccgggcggg tgtaggcggg
      601 aatgccggcc ttcctgccaa tcatggtgat ggacggcttg gtcagtccgt attcctctgc
      661 cagcatgtag cgcttttcgc cttgctggat tcgggctact atttcggcgt ttcgggccgg
      721 gatcggggtg tagtctttgt tcatttgtcc cgtggcgctg ccagccactt agtccaagcc
      781 atcgcggttt tctggttgaa aaacctcacg taatcgccgg ccaaactcat gccttttttc
      841 tgcgcatggt ctgtgaactt catcagctcg gctcttattg ctgctttgtc catgtctatt
      901 cctttcggtt gttgcgcccg gtgccggggc cgctcggcat caaagttagg cccagccatc
      961 agcaatcatg tccatcagct tgtcggctgt ctcgcggctc atggtcggca aaatgacttg
     1021 caacacgcca tcaagcgcgg cgctgtaaaa cacgtcgaag tcctcctgcc ccatcgcgtc
     1081 atagctgatc gaatgcggta tctttgtgac ctccccggta gtcgggtcga tgtgcggatc
     1141 gaagtaggcg gcggccagct tgatcgccac cagtgcctta gcctgcgtgt tataaacctc
     1201 gctgttttcc gtcaccaaat gcacaagcgc catgaatttc cggtggtgcg ggccattgcg
     1261 cgggcttgac cactccatgc gcaaccaggc tccgggtttc atggtttcca gcttgcgttt
     1321 gaacttcgcc cacagctcct gatctgccgg ggttgatccg cgcaaaccag cgggggactt
     1381 gattagcatg gctttcatgc agccctcgcc aattcttcgt tgtgccactg gatcaggaag
     1441 gtcttacgca tcagacggcc ctgctgtcca gctacccaaa tcctccagcg ataccatttc
     1501 aggatgtggg cgttatgcat gtgcttgcct cgctttcagt tctttggtct ttgccgtgta
     1561 ggtcgcctca atcgacttga tttcgtccgt ctcgtagtgc tttgcctcat gcgggccttt
     1621 cagccactcg acaagctcag cgccttcctg cgcaatcagg tttgcttcaa acgactggtt
     1681 taccgtgtag cccttgcggg cgtacttgcc agatccaccg ttacaggact tgcattgcag
     1741 ccagatattc agcggctcaa gggccaattc agggcgggcg cctttactca ggaagtgccc
     1801 gccgtcccat aagccgcccg gtttccatga gtcggttccg gtgacttcct cctgtgaccg
     1861 tccgcagctc atgcagcccc ggccctttgc cagttcttcc aggcggcgaa attgttgaat
     1921 ggccttcttt gcgcgggcca gccagtagcc gcgtggcttt aggatggaca gcttttcccg
     1981 tgtctcgcgc ttttccctga ccaggcgggc cttttcagcc ttgatgcgct gcgccctgcc
     2041 aatcttccca gcgcatacag ggccgcacac cttagcgcga agcaatccgg gcggggcaag
     2101 gaagttgcac tcacagacct tgcacacctt gagttttggc ggcttggtct ggatcacagc
     2161 aaccccctct gcacaggcaa gcactcgcgc cgcgccaaaa ccttccgacg aacggtagtt
     2221 gctaagcacg gattccgtgc tataatacag tcatggatta aattaatcca ccgcgcctcg
     2281 ggcacagggg caaaaggaaa atcatgactg ttacttacga atgggatatc gaagaaacga
     2341 ttgactacac cgggaaggac gatggcctta atgacgtgct ggaccatctt ttccagccag
     2401 atttcaaaag cctcaaaagt caattggacg aactcaaggc gcacgatgtt gaagatgggc
     2461 atgttcacta cgatcccgtg ctggtgcgcg acgacgacaa cggacggtcc tgggcgtact
     2521 tgatcgacgg caagctccct actcactttg aggacgccta tcaaaatcca gttgccaagg
     2581 tcccggcacg gtttcacaaa gaagttagca gcgcctgata tggcgaacca tccgaatcgc
     2641 agcaggcccc tggtggcctt ttcgccagag gccctgcaaa ctctccgaaa atctgcgggc
     2701 tggacgcagg aagagtgcgc agcacaggtc agcagcaccc gccggacact gcaggactgg
     2761 gaggctggca tagccgccat gcatcccggc ttatgggagt tactcaacat caaggcgcgg
     2821 agaaatcgaa aggtctgagc agccaccccg cgatgcttcg tcgcccgtga ctgcatcagg
     2881 tacacccttg cggcatggat ggatttttgg cggtcggtgg ggttcatgcg gcaacctttg
     2941 aacaccgctt tgccaactca acaagccaaa cagcaagttc tggcggggtg tgctcccttt
     3001 ccgcatctgg aatttccggg cgcgcccatg tcgctttttt atttctgtgt aagctagacg
     3061 ctacaacata cttagccagc ccaagcacca taggcatagg cggctcatca cccggccggc
     3121 atccaacgat gtaaagcaat gtggattttt cagccttatg cccgaaccag tgttgatgta
     3181 tcggtaaagt ccaccccccg taacaatcaa cctctccggg ctttggaagc cccatagccg
     3241 gccacaattt agatgcagat gggtgctcca aaaccccgcc aaactttcta atttggttga
     3301 cggcaaatac agctaactcc ttttcgtctg ggcggggttt tgccatatgt gacaagcggc
     3361 cccaagccct acatggagga tgggcaacta ttgggccgtc gccactccac gtcctggcat
     3421 cacggtcaat gtcgtaaacg tccacgtctg gcagcgtctt gtaaatcgag tcgctgcggg
     3481 caaaaagaac ggctactgtc atcccgccac cctcacaaaa tcagcacact gccgctcata
     3541 gaggcgcgaa aaatacacgt aggccggctg aagcgcgcag ccgctgaatc cgtgctt
//
LOCUS       DAIDFG010000316         3566 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_316_length_3566_cov_16.482199, whole genome shotgun sequence.
ACCESSION   DAIDFG010000316 DAIDFG010000000
VERSION     DAIDFG010000316.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_316_length_3566_cov_16.482199"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          558 a         1209 c         1198 g          601 t
ORIGIN      
        1 gttgtcgaaa gtcacggcgg ctatcgcctc gcgcagagag agatggatgc tgaccagcac
       61 ggtcttgagc tcgtcgttgg ccagcatcat gcgcaccggc aacgcggaca cgtccttgcc
      121 gaggaaagcc gcggcttcgg actgcaacag ttcggtgtgg ccgggaaaac gcgcaaacgc
      181 cgcgccggcg gcggccagcg cttccttgtg gatgggcgcg gtgaccatcg ccgcacactc
      241 gccggccagg gtcgcctggg ccgcccagac gatgcagtct gccgccgcct tgccagccaa
      301 agcactgatt ttcccaaaag ggacgggcgc aggcagctcg catttttgta gcacagggat
      361 gcagcgcggc ggcacggcct gccagtggtc ggccgcgtca agttgggcca cggatgacgc
      421 cagggccggg ccgccaccga ccagttgcgc ggcgcggcgc aggctcgcga catcacccac
      481 caccacgcag ccggccagtt cggccggcgc gtcgcgaaag gccctggcga tgatttccgg
      541 cccaatcccg gccggatcgc ccatggtgat ggccagtggc cgctttctca ggtcctgcat
      601 ctcttcccct ggcggtctgt acgtcccata atggtttgca tggcgctggt gcgtcacggt
      661 gtcacaagct gcttgccgac ccacctgacg gacaggtagc cggcaaggcc actgaacagg
      721 ggaaagcgaa gacatgcatc ctgccggtat tctcgcgcaa accctgacct gcgcccctgc
      781 cccatggtgg aaggcggcat gaacacggcc gaaaccatca tctggagcgc cgccgcagga
      841 tcgattggcc tggtggtcct gctcggcgcg gcagacgcgc ttgtcagccg gagccgcgcc
      901 agtgtgcaaa gcctggttta cctcacggct tgctggctgt ttttcacgct gctcagcggt
      961 cttgcggcaa ccatgctgcc acgccccgat gtgtcctggc tgcataccgc ccaggtgctg
     1021 atcggccccc tgtgcggggg tctgggcact tacggtgcga gccagtggct ggccgccgcc
     1081 cggcgcgacc ggctcaccga cctcagcttt ctcacggtca gcaccctgtg cctggcgggt
     1141 ggaccgctgt gcctgctgct tgcgccaggc ctgcagctgc cggcctcggc ggggctggcc
     1201 gtgctgagcc tgtcggtgac cttgtggctg gccgtgcgtg cggcgcagca gggcgaccgg
     1261 ctcgcttggg ggctggccgg tggctgcctg ttgacactgc cgctccagct cggcctgtac
     1321 tggctggtgc tcaacaccgg ccggccctcg gtcctgttgc agggcggcgt ggccctgctc
     1381 ggtttgctca gcgtctcggt gatcgccgtg actttgtggc tgcgcaacca ccaagacctg
     1441 gaaatccgcc atgccggcca gtcgcggcgc gatcccacca cccagcttta cagcagtgtc
     1501 gtgctggtgc agaaaatcct gcgtgcgcag cgccggctga cgcgcacccg gcgcgatggc
     1561 gcgatcatgg cggtacgggt gttcgagccc gagcggctgc tcgaccaggt gggccagcac
     1621 ggcctgaacg agatttacgc gcagctggcg cggcgcatgc agcgccacac cggcgtcgtc
     1681 aaccccgcgg ggcggtatta cgaccgctgt tttgtggtgc tgatcgagag cctgcattcg
     1741 cccggctgga tacgcaccat gggactgcgc gtggcctcca gcctgcggcg gccgctggag
     1801 atcagtgcgc tcaacggtga gcgcatcagg atcagcgccg acatcagcgt cggcatggtc
     1861 catttgacgg gtgcaggcag ggacgtggcc cagctgctgc acgaggccga ggagatggcc
     1921 gcggccgcac gcggcatgcg ctcgcgcgcc gcgctgctcg acgagatcag ccgccaggcc
     1981 gtgccggtgg aaaatgccca gctcggcgtg agctggcatg ccatgcgctc ggggcgtcag
     2041 caccacgcac gcaacaacgg ccacggcgcc cggccacgca ggacgccggc ctgacagcac
     2101 ccggtgctca ggccggattg tcgatgtcga tgaactcgtg cgccagcccg aactgcgcgg
     2161 ccacgtgcgc cgccacggcg ggcgcgccgt aacgctcgga cgcgtggtga ccgcaggcga
     2221 tgaaggccac gcccatttcg cgggcgtaat gcgcctgcgg ctcggagatc tcgccggtga
     2281 tgaaggcgtc ggccccggcg gcaatcgcgt cctcgaagta gccctgtgcg ccgccgctgc
     2341 accatgctat gtttttaata gctgctggcg cccgtccaac aagggtgacc ggccgattta
     2401 atgcattttt gacatgggag gccaggctct cggcgctgtc aaaaccttca ctggtgccac
     2461 tgcgcccgcc caggaaaccc agcttgttct cgccgaacca gcggtccgcc ttcagcccaa
     2521 gctgcagacc cagctgcgcg ttgttgccga gttcggggtg ggcatccagc ggcaaatggt
     2581 aggcgtagag gttgatgtcg tgtgccagca gcaggcccaa gcgctgtttc atccagccgg
     2641 tgacgcgtcc gtcctggccg cgccagaaca ggccatggtg cacaaaaatc gcgtcggcct
     2701 gcgcggccac tgcggcttca atcagggcca ggctggcggt gacaccggag acgatcttgc
     2761 gcacctccgc cttgccctca acctgcaggc cgttcggccc gtagtccttg aaagtctcgg
     2821 gctgcagcaa ctggtcgaaa gcggccagca gcaacgcgcg cggcgcaatc gcggagctgt
     2881 cagaactcat gggcacccca cctttctttt ccagccgggg ccgcggaacc ggcttcgccg
     2941 ggccgcaggc gcagcggccc cctcgggggg cagcgtagta cacggagtga caagcgtggg
     3001 ggtcatatct tcaccaggcg tgcagcgcat cgaccggaaa acgctgctgc ttgaccgcct
     3061 gcacataggc ttgcatcgcg ccctgcacgc tgtgggcttc ggccatgaag ttgtgcacga
     3121 acttcggcat tttgccgagg ttcatgccca gcatgtcgtg cagcaccagt acctggccgg
     3181 cggtgcccac accggcgcct atgcctatgg tcgcgcaatg ggtgagttcc tttgtcagcg
     3241 ctgcggagag ggccgccggc accatctcga gcaccagcat ggacgcacca gcgtcctgca
     3301 gcgcgtgcgc ctggctggtc aaggtggcgg cggcctcgtc gccccggccc tgcacccggt
     3361 agccgcccag ggcatgcacg gtttgcggcg tcaggcccag gtgggcgcag accggaatgc
     3421 cgcgctcgac caggaaatgc acggtctcgg tggtccagcc gccgccttcg agcttgacca
     3481 tgtgggcgcc ggcctgcatc aacaccacgg cgctgcgcag tgcctgctct ttcgactcgt
     3541 ggtagctgcc aaaaggcagg tcgcca
//
LOCUS       DAIDFG010000317         3544 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_317_length_3544_cov_15.965033, whole genome shotgun sequence.
ACCESSION   DAIDFG010000317 DAIDFG010000000
VERSION     DAIDFG010000317.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_317_length_3544_cov_15.965033"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          611 a         1114 c         1137 g          682 t
ORIGIN      
        1 aggacatggt cgccgggcgc caggtcgccg gtggagcgca tggtggccgc aatcatgtcg
       61 tcggggatct tgtgggcctg cagcaccgcc aggtcgtttt cgggctgggc gccggtgatg
      121 tgggcgatgg actccagccc gtcggaaaac accaccttga tggtctgcgc gccctgcacc
      181 acatgcaggt tggtcaggat gatgcctttg tccacaatca ccacgccggt gccgacgccg
      241 tattcctcgt cttccttgcc gttcctgctt ttgccgtaac cggtgacgcg caccaccgag
      301 ggacgaatgg tgtcgtaggc tttggcgacg gccgagggca actgggcggt ttccagcgtt
      361 ttcaggacgg ccgcatggat gtcggcctgc gtcaacctgc gcggctgcgg ccgcagcgcc
      421 agcgagaggc tgaggaccag taacagggcc atcacggcca gggcagccca aagaaggcgg
      481 ggactggaca gcgagaaaaa acgtgttgcc ggggcagatt cagcggctgg cccggcttgc
      541 gcctgctgct cgtgcgcctc cgccggaggg gggctgctgg agctgctgta gagggctggc
      601 ctgcgcatcc gtgtcacctg tgaaaagggt tttccgggga tttccggact gacgaatgca
      661 cggtatcacg tctgccccaa ccctgcacat tcttcaggca tccgtggggt catcgctgct
      721 ggccggtgtt caggcatcat cgggtcatgc cccgctggat agatactcat tgccacctcg
      781 atgcccaaga cttcgacagc gaccgtgtcg ccgtgcggga gcgggcggcg gacgccggcg
      841 tggtgcgttg cgttttgccg gcggtcgggg tgtttgactt tgcggccgta cgcacactgg
      901 cgcacgcttt tggggacagt tatgcgctcg gcattcaccc gctgtttgtc ggccaggcaa
      961 cagatggcga cctggccttg ctcgatgccg aactcaccct gcgaaaagcc gacgaccggc
     1021 tggttgctgt gggcgaaatc gggctcgact attttgtccc ggccctgaca caaagcccct
     1081 tgcgcgagcg ccaggagttt ttctaccgcg agcaactgct gctggcacgc aaacacggcc
     1141 tgccggtgat cctgcatgtg cgccgctccg ccgacaaact gctcaagcac ctgcgcgaac
     1201 tcgcgccgca ggacggctgg cgcggaatag cccatgcttt caatggcagc cggcaacagg
     1261 ccgatgaatt cctcaagctg ggcctcaagc tcggttttgg cggggccgtc accttcgagc
     1321 cggcgcggca gctcaggcgc ctggcggccg aattgccgct ggaggcgctg gtgctggaaa
     1381 ccgactcgcc ggacattccg ccgcactggc tgtacgccac ggcgcaacag cgcggcgagg
     1441 gccgggccca ggggcgcaac gagccggccg agttgccgcg cattgccgcc gtggtggctg
     1501 aactgcgtgg catgacggtc gacgccctgg cgctggcgac caccgccaac gccggcgcgg
     1561 cgctgcccgg gctgcaggcc ctgtccgcat gctgaccggt ctggcgccgc tggtcagcag
     1621 ccggacccgc gtgctcatcc tcggcagttt ccccggcgtg gcctcgctgg cggcgcagca
     1681 gtactacggc cacccgcaaa accatttctg gaaaattttg caggccattt ggccatccag
     1741 cccaatatca tcgggcggag acagctatca aaatcgtagc aaatggctat tggacaggca
     1801 actgggcgtc tgggacgtgt atgcctcctg cgaacgcgag ggcagcctgg acagccgcat
     1861 ccgccagccg gtggtcaacg atttcgcagc aatgctggtc cagtgcccgg cgctggaagc
     1921 cattgctcac aatgggggcg agagtttcaa acacgcgcgc cacaccgcgc tgctgggctt
     1981 gcctgtctac aagttaccgt ccaccagccc ggcgaacgcg tcctggtcgt tcgagcgcaa
     2041 gctggcggcc tggcgcgaag tcttcgaaaa gcacggcatg gtgtgatcta gcgcagcgtt
     2101 gcacgctatc tggaacataa aagcgcgtct tttgtgccat ggctgcgttg caaatccgcg
     2161 cgatacctag ggtatcgctg tggtttgcgc cttgccctga cccaacatcc atcgctttta
     2221 tttgttccat caagcatgca acactgcact agcgtccggc atgcgccgcc gtgccaggcg
     2281 gcagtgggtg gatttatcca atcggcccga tggcgttgac cgtctggcga tagtcggatt
     2341 cgttgagaac gaagtcgccg cgatcccagg ccagtccgcg ctgcatgcca tctgtctttg
     2401 caacgttgat gcgcgtgacg tggggctcat gaatgccgac caggttgacg gcagggttga
     2461 acgccagaaa cacgccgcgc agcgtggcct gccggcgaag tcgccgccgc ccgttctcca
     2521 tgtggaactg cccccattcc ttcatgcacc acggactgtc ccggtattcg tccgtgtaca
     2581 caaaaagcat gacttgcgcc tccatcatgg cgatcttgta catcctgttc cagtcggatt
     2641 tcattgcgcc tatgggccgc gcgcctgtgg gggagcgcat gtgcggccgc atgtcgagcg
     2701 acacactcgc cacgccgtca ggcgcgatgt cgttctgcgc aaatgttttg gcatggcgcg
     2761 tcgcaccgga ccgggccacc acggagtcca tgtacaccgc gtccacctgg tacaggttgt
     2821 actgtttcat caagcggtca cgcagccagt tggcgaaatt ggccccgtgg ttggccatgg
     2881 caaaactgag cagaattttt gtggacaccg gttttccttt ccctggatgc gccgcattat
     2941 ggaaaagttg tccggcgcgg tcaaattttt cccggaagat ggcgtgccag cgccatgccc
     3001 ttccctgtgg ttgcgggcac ttgcggccgg tgggtacact ttgagccttt gacgctggcc
     3061 gttgaacagt caccctgttc cgggcgggcc gacaagcccg gccgcgttca gcgccagtat
     3121 ccagattgaa ccgatggccc gcaaagaaac cagcaacccg cttccccagg cagaactgcc
     3181 cgaggtgaat ttctccgatt acggcgacgt ccgctacctg catctgggca cggagtgggt
     3241 gcaggggtcc atgctgctgg acaaaccttt cgacatcgag ctggagtatg tccagcgcat
     3301 gatggtctgg ctgctgttcg ccgagccggg cacggtggcc gcgcgccacg cgatgcagct
     3361 gggcctgggg tcggcagcgc tgaccaagtt ctgctacaaa aagctgaaga tgaagaccac
     3421 ggccatcgag ctcaacccgc aggtgctggc cgcctgccgg ctgtggttca agctgcccgc
     3481 cgatgacctg cgcctgaagg tcatcgtggc cgacgccgcg caggaaatcc gcaaggcgga
     3541 gcac
//
LOCUS       DAIDFG010000318         3540 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_318_length_3540_cov_16.610043, whole genome shotgun sequence.
ACCESSION   DAIDFG010000318 DAIDFG010000000
VERSION     DAIDFG010000318.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_318_length_3540_cov_16.610043"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          597 a         1204 c         1174 g          565 t
ORIGIN      
        1 gcgcgccgca gtgcggccac gaaggccggc tccagcatgt ccagcagttg ctggtcgtgg
       61 tggttgaact cgcggccctt gcgcccgcgc cagatgcgca ggtcgcccag cgcctcgtcg
      121 ccgtcgaagg catgcaggtt gatgccccag tgcaggccgt cgcgcgccag gaagtcgttg
      181 aagaactcgg ttttcaggaa ctcattgcgc ggcatcacct cgctgaccag ggtggcgtgg
      241 cggcgcgact gcagcgcgaa ggtgatgggg tcgcggtact ggtaccaggc ctcgtaacgc
      301 tgcaggttgg ccgggtccat gttgatggcc acggggtggg cgaagcaacc ggagggctcg
      361 ctccagatgt aggaggcgaa gtattccgca cggaacagcg ccagcgtggc ctgacccatg
      421 cgctcgcgga tttcgcgctc attgaggtcc tcggccagca gcgaaaagca ggtggccagt
      481 gcgcgttgct cggtgctgga cagaaacatg gcgggcctct atgaaggtgc agaaggggac
      541 gggatgctgc ggcaagccta gcgccaacgc cggcgccggt caatccgccg tgtcatcgaa
      601 ttcgatgaca gacagccggg gcggcagccg ccatgatcgc gctgcaagca ctgaacccgt
      661 tgaacccact ggagtgacca tggcccgtct ttcccttcca catacctgca gcacgctagt
      721 ggctgccgcc gcgctttcag cctgcagcgg ccctggcggc ctgcgcggcg cgcccgacat
      781 cgacagcctc accgccaccg aagccgcggt ccgcatttgc agcggcacgc tgagcagcga
      841 ggcgctggtg caggcctacc tgctgcgggc caagtcccgg cccgagctga acgccttcgt
      901 cacgctggac gaaaccggcg ccctgcaggc tgcgcgcgcg gccgaccagc gccgcgcagg
      961 cggcggcgcc tgcctgccgc tgcagggcgt gcctatcgtc gtcaaggaca acatccaggc
     1021 gcgcggcctg cccgccacgg ccggcacacc ggcgctgaaa aactttgtgc ccaccgccga
     1081 cgcacccgtg ctgcagaagc tgcgcgacgc gggcgccatc gtgctcggca agaccaacct
     1141 gcatgagctg gcctttggca tctcgggcta caacccggcc ttcaacaccg gcccccagcc
     1201 cggtgtgcgc aacgcttacg acgtgacaaa gatggccggc ggctcgtcgg ccggcagcgc
     1261 ggccgcgctg ggcgcgcgca tggcacccgc cgggctgggc accgacaccg gcggctcgct
     1321 gcgcgtgccc tgcgcactga atggctgtgc ctcgctgcgg cccagcatgg ggcgctaccc
     1381 gcaagccggc atcgcgccga tttcgcatac gcgcgacacg gccggcccga tggccgtggc
     1441 catggccgac gtggagctgc tggaccgcgt ggtcaccggt gcgcccgcgc tgctgcctgc
     1501 agacctcaaa gctgttcgcc tcggcgtggt gcccggcttc ctggcgaatc tggacagcga
     1561 cacacgcggc gcctgggaaa catcgctggc ccggctgaaa gcggctggtg tcaccgtggt
     1621 cgatgtcgcc atgccgcaac tggccgagct caatggcaag gtgggctttc ccgtggcgtt
     1681 gtacgaagcc tacgacgaca tggtggccta cctggccagg tacaacaccg gcgtcagcat
     1741 ccgccagatg gcagcaagca tcgccagccc ggacgtgaaa ggcacttacg acgcgctggt
     1801 gattccgcgc aagctgcccg ggccgaacgg cctggttgat gccgcgccca tttaccaggc
     1861 cgcgatgacg gtgcaccggc ccgcgctggt caagctgtac gccgacacct ttgccggcca
     1921 gcggctcgac gcgctggtgt tccccaccgt gccgcgcacc gcgctgcccg ccacgcccga
     1981 ggccagcagc ctggagaact tcggcctgtt catccagaac accgaccccg gcagcaacgc
     2041 cggcattccc ggcattcagc tgcccatggg cctgggcgcc agcagccgca tgccgacagg
     2101 gctggagctt gacgggccgg ccggcagcga cagacggctg attgcgattg gcctggcgct
     2161 tgaaaaggtg ttggggcgcc tgccggcggc gcgctaagca ttcgccgtga gcctggcctg
     2221 tcgaggggtt gccacaggtc aggctgtcga gattgctact gttttaatag ctgccggcgc
     2281 ccattggtaa aaggctggaa gcctttttga tttgaagttt tcagtcaagg cgcttccagc
     2341 cagcctgcac cctcagccag ggcggcactt gcaagcgcgt gagcgtccgg cccgcggctg
     2401 gtcctcgtta tgatcttgcc cggggcgcct cctgcaggct gcccccgccg actgtcacgc
     2461 accgggccgc accctgctgc tgccgcgtga cttccttttt tttgacgacc cagccctttc
     2521 tgcgtggagg gctggagacg tttttttgcc agccatccct gacgaactca ccatgaacat
     2581 cacggtcaac ccggaactgc ttgcctacat cgacccgctg acagccgacg aacgcgaggc
     2641 ccttgaacgc agcctgctgg ccgaaggttg ccgcgatgcg ctggtgttgt ggggcgacgt
     2701 gctggtggac gggcacaacc gccacagcat ctgtacccaa cacggcatcc cttttcatac
     2761 cgtgcagaac agccatttca aatccatcga cgacgtgcac ctgtggatga ttgaccagca
     2821 cctggggcgg cgcagcgtgt ccgacttcca gcgcggcgtg ctggccctgc gcaaaaaaga
     2881 catcctggcg gcacgcgcgc aaacgccggc ggaggcgcct tccgagtccg gccaggcgac
     2941 agaggccccg accccggcgc ccgtacccct gctgacgcgc gacgcggtgg cgaaagccgc
     3001 gcgcatcagc agcaacacca tcagccagat cgagaaaatc cagaagtcgg ccgcccccga
     3061 actgatggca gcggtgaagt ccggtgccat ttccatcaac gccgcggcca ccgtggcctc
     3121 actgccggtc gaagcgcagg tggctgccgc cgccggcggc cgcaaggagc tgcagcaagc
     3181 ggcccgccag gtgcgcgaaa cccgcgccgt ggcgcgcacc gaacccgagt cgcccgaggg
     3241 ggtgattgcg cggctcaggc agaccatcag cgaactgatg gcggaaaacg ctgcgctcaa
     3301 ggcgcagctg gctgggcaag ggcgctgagt tggcagcacc gttgcgtgcg cctctcaacg
     3361 gttttgatga gtcctacagg cttccggccc ttatataacg ggcgcaagaa gctatcaaat
     3421 agtgagcatt cgggcatcac cagcaactcc gcctgcgtgc cactttgagc agttttgacg
     3481 cgattagtag ttagtggagc agggcgtggg cgcggcggtg gggcctgctg ctgcgttggg
//
LOCUS       DAIDFG010000319         3533 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_319_length_3533_cov_16.617596, whole genome shotgun sequence.
ACCESSION   DAIDFG010000319 DAIDFG010000000
VERSION     DAIDFG010000319.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_319_length_3533_cov_16.617596"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          689 a         1155 c         1063 g          626 t
ORIGIN      
        1 gacaaacgga ttaaaagtcc gctgctctac caactgagct aaggccccaa ggatttctcc
       61 ctggtctgta ctgcgtaatg cagaacagcc tcaaattata gcgtcatttt ttggctcttt
      121 aaaaaactac tcagcaattt tgttgagcac ccaacctgca gcacacaaac cgaaggccga
      181 tgtcacgctg accaccgatc catagccatg acaattgagg ctgccatcgc ccgatgcatc
      241 gtcctgaatc tcgcatgcat ccagcgcagc gtcagccccc gaggccaggg ccgcgggctg
      301 catcaccgcc tcgcggctga acacacaggc cacaccgatc tttcccttgc gcgccgcgcc
      361 ctgctccctg cgcaggcggt aacgcagctg tgcgagcagc gggtcgtgcg tgaccagcga
      421 caggtcttca atatcaacct tgtgcgcgtg gcgcttgcca ccggcagcac cgacgctgat
      481 gaagttgact ctgtggcgca tcgcccatgc ggccatggcg gttttggccc ggacctggtc
      541 gcaggcatcg atgaccgaca ccgcgttgct gccgtgcgca tccagccctg caatcatcgg
      601 ccagttggcg gcatcgacaa actcctcgat gcaatgcacc acacacccgg gatggatgtg
      661 cgttatgcgt tcgcgcatcg cctggacctt ggcctgcccc agtgtggtgt cgagcgcgtg
      721 gatctggcgg ttgatgttgg actcggcgat gtggtccagg tcaatcagcg tcagcctgtc
      781 cacgccgctg cgcgccaggg cttcggcggc ccaggaaccc acgccgccca ggcccaccac
      841 taccacatgg gccgcgcgaa ttttttttgc gcctgacacg ccatacagac gcgccagccc
      901 gccgaagcgc cgctccaggt cggcagggag ttcagaagaa atttcagtca caggcaattt
      961 cccgccggga cgccccaagg gaaatcagcc ccctcggggg gcagcgagga cacgccagtg
     1021 ccgagcgtgg gggccatcaa ctttccagcc gccagatctg gtatttcagc cccgccacag
     1081 caccggcgat gatggccgcg acagccacga aactggtggc gctcagcgtg gagatgccgg
     1141 acaggccctg cccgatggtg caacccatgg cggttacgcc gcccaccccc atcagcacgg
     1201 ccccgaccag gtgattggcg gtgtcttcgg tatttcgaaa gccttcccag cggaagctgc
     1261 gtgacagcag ggaatacagc gccgagccaa cgaccacgcc aaacacggac acaacaccca
     1321 gcgtcaacaa tttggtcttg tcactgaaaa atatcagcca atcgatggtg taggccatcg
     1381 gagccacaaa actcagggct tccatgcggc cggaattggt caccagaaac gtctcctcca
     1441 gcgtttccgg atgctctgcg acataagcca tgtgaccgct gagccaccac atggcggtaa
     1501 tcagcaggcc aatgccgagt ccggcgagca ggttgtcaaa gcggagaaac tccgggccgg
     1561 ccagcgccca gacgatcagg cccccaccca gcaccccggc cagcaccagt ccggccagca
     1621 ccggtgacac accaagcaaa ccggtcgccc agttggacaa ggcggcgttg ccggaaaaat
     1681 cgatggccac acgatctacc gtggccacgc gcagcaccgc cgtgatgccc ttgagtgtgg
     1741 caaatgcggc caccgccatg accacaaaca ccaccagtga tttgaggttg ccgccaccta
     1801 tgcgcaccag cgtcttgctg ccgcagcccg aagccagcac catgccaaag ccgaacagca
     1861 ggccgccgac cagcgcggac agccagatga agcggttcga ggcatatatc gctttggccg
     1921 ggtcgatcag cccggccgcg gccaggccgt aaaagccgat catggccacg ccgatggcca
     1981 tgccccacac acgcatgcgt gtccagtcac ccatgttgac aatgtcgctg accgcaccca
     2041 tggtgcagaa gtgggttcgc tggacaacgg cgccgaacag caccgccaga atgaaggccg
     2101 cccagagaac ctgggcggtg agtgtgttga gctgggagat atccatcgcc cgattttagg
     2161 ggctgggcag gaagtgcgct gccctgccgc ctgcgaagtc tctacttgag cctggccagg
     2221 cgttccttgg cgaccgaagc ggcttccgac tgcgggtagg ccttgagcag gtcttccagc
     2281 gtcttgcgcg ccgccttgac gtccttgagc tcgacctggc agttggccac ggcaagcacg
     2341 gcttcggggg cgcgcatatg ctcggggtcg gcggccacca gcgcgcggaa attgaccagc
     2401 gcgtcgcggt agttgcgcag cgcgtactgt gcgttgccga gccagaacag cgccggcgcc
     2461 ttgtagccgc tttggggata ccgcttgaga aaattggtga aggcggcctg ggccggcgcg
     2521 aactcgccct tgcgcagtac tgccagcgca gcctcgaact cctgcttttc tgccgggtct
     2581 gccacgaact cacggccgtc aacactgacc ttggatggct cgaaccggct gagccggtca
     2641 ttcacgccct gtgacaaatc cttttgcatg cgctggactt cggcgagttc gcgggcaaac
     2701 tgctcgtcct gcccgcgcat tctggccagt tcgctgcgca actggtcgat ctggctggac
     2761 aactcgagca ggctgcggcg cagctgcgcg gtttcatcac cggacttgcg cagctcggtc
     2821 gttgtggcat cgagccgctg gcgcagttca agaatcgcct tgcgggcttc atcatcctcg
     2881 aacaacgccg catgggcgtt gaacgccatg acgcaggcta gcgcacacgc cacggctccc
     2941 agcgaagtcc ggaagttctt caaggtcatg gctgcaactt aacggtagtt gaattcggcg
     3001 cggcgatttt tggagtagga ctcttcatcc gagcccgtca cggcaggttt ttcctcgccg
     3061 aagctgaccg cttcgacctg cgcatcggtg gcgcccagca ggcccaaggc gcggcgcacg
     3121 gcctcggcac gtttctgccc caatgccagg ttgtactcgc ggccgccgcg ctcatcggtg
     3181 ctgccttcaa tcaccagctt gcgggccttg ttggcgctga ggaaacgcga atgggcttcc
     3241 accgtcgcct gaaattccgg cttgatgaca aaactgtcgt agtcgaagta gatcacgcgg
     3301 gctgcaccgg gcgggccagc gagcgaagga tcggtggcac cgatcaggac aggcgccaca
     3361 tcgcggccgg tcacgccggc tgcgtcgccg gttcccggtg cggtggtgac cggggtccgg
     3421 ttttcgacag gcacctcatt gagtttgacg cccgagctgc agccgaccag tgcggcgctc
     3481 agggtgatga tggaggcgag tgagatcagg atgcgcttca tgaatgtgta ctt
//
LOCUS       DAIDFG010000320         3532 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_320_length_3532_cov_21.800978, whole genome shotgun sequence.
ACCESSION   DAIDFG010000320 DAIDFG010000000
VERSION     DAIDFG010000320.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_320_length_3532_cov_21.800978"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          634 a         1221 c         1118 g          559 t
ORIGIN      
        1 caacccggat ccgctcaagg tggacccggt ggccacggtg atcgacggtt gcgtcggttg
       61 cggcctgtgc ggcgccaacg cgcatgcggc gaccctgtgc ccctcgtttt accgcgccga
      121 agtgatccag aaccccaagt ggcatgagcg gctcctgggc agcgtgcgtg gtgcactggt
      181 gaaagcgatg cagacatgac cacgactcaa caaaaacccc tcaccctcct gatctgtgca
      241 ctcggcggcg aaggcggcgg cgtgctgacc gaatggctgg tcgacatcgc gctggccagt
      301 ggttacgccg cgcagagcac gtcgattccc ggcgtggccc agcgcaccgg cgccaccact
      361 tattacctcg aggtgttccc ggtgccgctg gccgagctgc agggcaaacg cccggtgttc
      421 agcctgaacc cggtgcccgg tgcactggac ggcatcgtct cgtccgagct gctggaaacc
      481 gcgcgccaga tcggcaacgg catgagctcg ccgctgcgca ccctggtcat cagttccagc
      541 agccgcacct tcaccacggc cgaacgcatg gagccgggtg acggccgcac cgacagcgcg
      601 ccgctgctgg aagtggtgcg tgcgcaaagc cgcgagcacc atgtgttcga catgggcgaa
      661 ctggccagaa ccagcggcac cgtggtcagc gccgtgatgc tgggcgccat cgccggcagc
      721 ggcctgctgc cttttgcgcg tgaggcctac gagaccgtga tccgccacgg tggcaaaggc
      781 gccgaagcca gcctgcgtgg cttctcccag gcctttgaac aggtgggcac gcaacgggcg
      841 cagacggcgc tggtcaccag cctgctatca gaagaggagc tgtcttcgcc cgtccccaaa
      901 gggctggagg ccaatttggc taaacttttt ccaccaccgg cgcatgacat gctggccctg
      961 ggccacgccc gcatgctgga gtatcaaggc cgccaatatg ctgacctgta tgtgagccgc
     1021 ctgcggcagg tgctggccgc cgaggtgatg gcggacccgg cggcggtgca aggctacgcc
     1081 accacacgcg agatggcacg ctggctggcg ctgtggatgg cgttcgacga catcgtgcgt
     1141 gtcgccgacc tgaaaagcag cgccagccgc tggcagcgcg tgcacggtga ggtcaaggcg
     1201 ggcgccggtg acctgctgca ggtttacgat catttcaagc ccggcgccgc ggaattcgca
     1261 gccctgctgc cggcgggcct ggccgcgcgt gtgacacgct gggaccgcca gcgcgtggcg
     1321 cgcggcaagg cgccgtgggc cctgccgctc aaggtcggca cacactcggt cttcggcatg
     1381 ctctcgctgc gtctgctggc cagcctgaag tggctgcgcg tgcgcggcag ccgttttgcc
     1441 accgagcagg cgctgatcga gcagtggctg gccggcgtgg tgcgcggaac gcaggcgcac
     1501 tggcaactgg gccacgagat cgccctgtgc ggccgcctga tcaagggtta tggcagcacc
     1561 aacgaacgcg gcaaggacaa cctcatgcat gtgctggagc acctggccac gggcgccgct
     1621 tttgcatcga acgaactacg cgcagccgcc atcgccgccg cccgccatgc cgccctgctc
     1681 gatgaagccg gcaaggcgct ggaccagacg ctggtgagcc acggcgcacc ggcgcgcccc
     1741 gtcaaagcac aaccgatcag atgggtggcc aacccacacc gcaagccgcg tgccgccaag
     1801 agcacctgat ttttttaccc acccaagagg agacaaacca tgagcccatt ctttcaaccc
     1861 ggccgccgca ccctgctggc actggcgctg atcgccagca gcctgttttc cgcagcccag
     1921 gcgcagacct ggccggccaa gccggtcaag gtcatcgtca acttcccgcc cggcggcgcg
     1981 gccgaccagc tggcacgcgc catcggcacg ccgctggccg aagccctgag ccagccggtg
     2041 gtggtggaaa accgcggtgg cgccaacggc aacatcggcg gtgaagccgt ggccaaggcg
     2101 cctgccgacg gctacaccct gctcatgagt tcgggcggca tggtgtcggt caacccgcac
     2161 ctgtacccga agatgtcctt caatccggcc aaggacctgg tgccggtggc ggccgcggca
     2221 cgcgtgctgg tgttcctgga agtcaaacca acgcttccgg tcaacaacgt gaaggaattc
     2281 ctggcctacc tgaaggccaa ccccggcaag ctgtccttcg gctcaccggg caacggcagc
     2341 tcgccacacc tggcggccga gatgctgaag gcccagacca acagctttgc ggtgcacgtg
     2401 ccttaccgcg gcgcggcgcc ggccatgcag gacctgctgg gtggccagct cgacttcatg
     2461 ttcgacccgg gcatcggcct gcagcatgtg aaggccggca agctcaagct gctggccgtg
     2521 ggcagcgcca aacgctcgcc cctgtttccg gatgtgccca ccctggacga agccggcctg
     2581 aaaaacttcg acgccgacac ctggttcggc ttttacgcgc cggccggcac cccgccggcc
     2641 gtcgtgagcc gcctgaacca ggagatcaac aagatcctcg cgacgcagcc ggtgaaagac
     2701 cgcatcatgg ccataggggg cattccggcc ccgatgtcgc cgtccgactt caacatgcgc
     2761 gcagccattg acggcacacg tttcggcgcg ctgatccggg cccgcaatat caaggctgaa
     2821 tgagtaaggc ccccacggtc gctcacaccc accgttcggg ctgagcctgt cgaagccccc
     2881 cttcgacaag ctcagggcga acggaccgga agcctgacga cataattcac ccatgagcaa
     2941 acagatcacc cacaccatcc gcgttgaatt cggcgactgc gacccggcgc gcattgtgtg
     3001 gtttcccaat ttcttccgct ggatagatgc cgcctcgcga aacttttttg tccaatgcgg
     3061 tgtgccgtcc tggcaggaga ccgaaaaaac aatcggcgtg atcggcacgc cgctggtgga
     3121 cacgcactcg cgctttctgc gcaccgccac ctacggcgac atgctggaca tccacacgtc
     3181 catccccgaa tggcgcgaga aaagttttgt gcagcgttac cgcatcatgc gcggggacga
     3241 cgccatcatg gaatgtgacg aggtgcgcat ctttgcaggc cggcgtgccg acaacccgga
     3301 agccatacgg gcgttgccga ttccgcccga cattcgcgcg ctgtgcgagt agagccggac
     3361 cagcgaccgg ccagctaccc ctgatttgtc attgcgggca accccctgcc cactgtcgtt
     3421 ggcaaggtgc ggcacccgga cgtgccacag acagcaggac ttgactcccc actccctccc
     3481 ctaacccctc cctcgccccg ctgggcgatg gagggggact tcatttggcc gc
//
LOCUS       DAIDFG010000321         3531 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_321_length_3531_cov_8.277330, whole genome shotgun sequence.
ACCESSION   DAIDFG010000321 DAIDFG010000000
VERSION     DAIDFG010000321.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_321_length_3531_cov_8.277330"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          522 a         1060 c         1202 g          747 t
ORIGIN      
        1 atgttgtcga tgccttcctt gatatcggac tgcgggttgc tgttgacaaa atcggaccac
       61 tgagtgacgc cgatcagcgt ttccttgttg gtcgcgccgt agttggcagc gtcaaacacc
      121 aggccggcag cgcgcacttc acgggccagc acgatcagct cggtcaggcc ctgcgctgcg
      181 cgcatcttgg ggtcaggcat gccgggctgt gcggcggcgt tcatgctgtc gctgttgggc
      241 acgggatcat ccaggccgtg gtcgtcggtg ctggccgtga cttcagtgcc ggtgaactcg
      301 acctggttgg gctggccctt gcggcccact ttggtctcgg gcgggctgaa aaactcgccg
      361 atgggatagc tcagatactt gaagttctgg gcgtccacag gcacacgcgg caggacgttg
      421 tcggcgatca tggcgccgtt tttgtacgcg acggcgaccg ccgacagtac aggggtaact
      481 acaaaaggcg ttttcatgtg tggtcgctcc aggttgtttc agtaaaaaag ttaaatcaga
      541 aaagagagtc gggatcagga agcggctgcg ggtcagccct gcacgtagcc gggagcgatg
      601 tggtaatcga tcacgtcgtc agccacaccg gactgcagcg caaagccgat gattcggttg
      661 ttcgcgccag cggctggtgc ggcggcgacg cctttggagg ccgcgtctgc cgtgatcggc
      721 tggccgcgtg tgacgatgcc gccgagcttg acctggccgg tgcctgcggt ggggtagtcc
      781 acctgctcgc cgatttcctt gggcagggcg tcgttggtgc cgagcagcag atcggtcggc
      841 gccgccgcca gcgcggcgct gttatccacg gcgccgtgct tgatgatcag aaagcccgcg
      901 atgagggcct gggccaaaac gctgcggtgt tgaagaggtg ccattgattg ctcctagaaa
      961 aacaggttga aaggttgggt tgaggttgtc ggggtctggg tagcgcgtcg gggtctgggc
     1021 ttacttttcg cctttggcca ctgcgaccac gtgctgcaca gcctgagcgg cggaaacgtg
     1081 gcggcccttg ccttcctcgg cggccatgaa gtcgctggcg gcctggctga ccgctgccgt
     1141 gtcgctcacg tccagcgcgt caccctcgtc ggcctgcttt ttggcgccga gcttgatgac
     1201 gggatcgcgt gcggccatga actcggagaa aaactccagg gcggtttttt tctgctcgcc
     1261 gccctcggcc ttggcaaaac tgaattcctt gccctcgtct tccagcgcgg ccatgtactc
     1321 ggacaggccg gcctcgtcgc ggggcagaat cacacccttg gccttccact cggcgatctg
     1381 ggtggcgatg cgggttttct gctcggcctt tttgatggtc agcagctcgg cttgggccgc
     1441 gctgaagtca gctgcaggga ccatgccctc tttgcctgcg gcctcgccct cgctgcgggc
     1501 ctgggcaatg gcggcgtcgt gttgctcttg agtgatcatg tggtcttcct cggtcggttg
     1561 gttgaaaagg gatgcaggtt tgtcgtcgtt gaactgggtg cgggctgtgc tggccgcctc
     1621 agtcatcgag tcgatttccc actgcggcag cgtctcgtcg gcggcctcca ggccgtcttt
     1681 ctcgatcatt cggtcgcgca ggcggcgcag cagcttggcg gcgctctcca ggccccagac
     1741 cagggaataa ccgggcgcgg caaattcaaa gcaggtttcg tcgtcggctg cgaactcgac
     1801 cggcgccagg ccgtcgatgg cgggcggcat ggcgccgagc cagcccacat gccgcacgcg
     1861 ccagccgtgg accttgtcct tgatcaccga gaccgaacgg ttgtaatagg cgccggactc
     1921 gacgcccttt tcaaaggccg ggttgatctt gtcgaacttg gcaaacaggc ggccatcctc
     1981 gtccagcttg tactggtcca cctgggcgta ggccggggcg gtgtccttgg ggtggccgat
     2041 cacggcgggc gcggcgccga gcttgtggtt ttccaccatc tgctcgacgt cggccttgct
     2101 gaagctgacc tgccggccct tgctgtcggt gtgcgtgcct accttgaaaa cttcaacaaa
     2161 cccgcgcagg cctttgacgg cggcgtcgat ggctttgctg atcggggctt tcttggatgt
     2221 gggcgatttg ttcatgccgc tactgtgccg agcggccccc tttaaatctt ggtgaagcgt
     2281 ttcaccatgc aggggtttgg agcgggaagc aagagcccgt ggcgggcctg gcctgtcagg
     2341 cggccttggc gacctcggcg gctgcggcgc gggccttggc gcagcgtgcc tccagatcgg
     2401 cgctgaccac ggacaaaagc atggcgaggt cgcggcgctg cagctgcggc aggctttcct
     2461 cgctgccccg gccttcatta ctggtgagct ggtggattgc gcgcagggcg ccaagctggc
     2521 tttgcaactc gtcgatggcg tctacgactc ggaaggcctg acgctggctc tcggttgtga
     2581 cgtggatcgc ctgcacggcg ggctggtcag gggaggaagc tttgttcata aatgccgcgt
     2641 cgtctcaggt ttaacaacgc cgcaatcatc ggggaatgat ggcggcggcc cgaacaggtg
     2701 tgcaagaccg ggagacgctt ggcgttaccg gcaggagctt gcgcccctcc cattcgggcc
     2761 gcctaaactt ggtggcccaa aggaagaagc cgcatacctg cggtggttgc ggcctcttgc
     2821 cgcgtctcat cgggcttgca cacccggtca cccgctattt gcaagtgacg tctgaattat
     2881 cggccattgc ccgctcatgg cgctgcgccg ttacttttcg gagtggtatt ttgggtacgc
     2941 cggggaaagc ccctaggcgg cgctcaaggc gcttttggct ccgctggtgc cggtgcagag
     3001 gctgcggcgc gttttaaagg ggtttctggc cgttttgaaa gcggttccga ccgcccggcc
     3061 tggtcgctag gcccgtttgc cgatcccgaa ggggtcgttg gcctgaccgg cgtagttcgc
     3121 gggctcatcc agctcggcaa acatatcacg cgttttcatg tccgtcatgc cggcgtcgcc
     3181 gcccatgatg ttgatgcact ggcggcggct gtagccggtg acctctgcgg tctcgtcaaa
     3241 ggtcaggtcc tgcgcacggc actggcgcac gtggtcatgg cgcaggcggc gcaggtaggc
     3301 gtcgcccttg ggcacctgca gctcgccccc gccgtactcg gccagcagct tcacaaagcc
     3361 ttcagggctg agcgccatgc gcagcgggtg gtcgtccttg gccctgccgg gcttgggcac
     3421 cgatatgcgg gcgcccggca gcgagccgat caatcgcatg gcgtcagcgt cgcccagcac
     3481 gcgggccagc tccctgatcg ctgcaggcag cgcctcgggg ctggtcgtga g
//
LOCUS       DAIDFG010000322         3529 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_322_length_3529_cov_19.026195, whole genome shotgun sequence.
ACCESSION   DAIDFG010000322 DAIDFG010000000
VERSION     DAIDFG010000322.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_322_length_3529_cov_19.026195"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          595 a         1124 c         1167 g          643 t
ORIGIN      
        1 cactgcgtgt agctccctgc cccccgaggg ggctgctcca ccttggggcg gcccggcggt
       61 ggagcgtatg ccccccacgc tccacactga gttgtcgtcg ctgccctggg gtgtcatccc
      121 ggacctgatt cgggatccat gcttgattga ccactgacat gagtgtgccg gcatggattg
      181 cgggtcaggc ccgcaatgac aggcaaaggg ctggcggccc tgcgcggcgc gcgcccgacc
      241 gcggcacaat caggccatga gaatttcctt ttgttgtacc gataccaagg ccgagccctg
      301 gctggccggc ttgcgcgccg cttttccgga tgcgaatgtg gaacagtggg cgccgggcgc
      361 cgcgccggcg gactatgcgg tggtctgggc gccgccgccg cagtttctgg acgagcagcc
      421 tcagctcaag gccctgttca atatcggggc gggcgtggat gcgctgatga agctgcgtct
      481 gccgccacag gcggccgtgg ttcggctgga cgatgccggc atgtcggtgc agatggccga
      541 gtatgtatgc catgcggtga tcaggcattt tcgcgagttc gatggctacg aggccgatgt
      601 gaagcagggc acatggtcct accgcaaacc tcggctgcgc aaggacttcc cggtcggcgt
      661 gatgggtctg ggcgtgctcg gcgaacgcgt ggcccgggcg ctggcgcagt ttgactaccc
      721 ggtactgggc tggagccgat ccgccaagac gatagcgggt gtgcagtgtt tttcagggca
      781 ggccgggctg gatgatttcc tgtcgggcac acgcgtgctg gtgtgcctgt tgccgctgac
      841 ccctgacacg cagggcatca tgaaccggca aacgctgtcc aggctgcggc ccggcggtta
      901 cgtcatcaat gtggcgcgcg gcacgcacct ggtggacgag gacctgatca cgctgctcga
      961 cagcggccat ctggccggcg cgacgctgga tgtctttcgc accgaaccgc tgccggccgc
     1021 ccatcccttc tggaaacacc cgctcatcac cgtcacgccg cacacctcgg cgcgtaccct
     1081 gcgcgaggaa agcattgcgc agattgcggg caagatcgcg gcgcttcagg cggggcatcc
     1141 gatttccagc cttgccggtg tggttgaccc gaaaaaggga tactgatgtt gcccccacgg
     1201 tcgttcgctt cgcgtaactc cctggccccc gaggggcccc tgcttgattt gagagatcac
     1261 ggtcacccac cgcgcatggc tccctgcccc tatttcgtga aagcatattt atgaacctcc
     1321 cctccaaagt caaaatcgtc gatgtcggtc cgcgcgacgg cctgcagaac gaaaaggcgc
     1381 aggtgcctgc cgccgtcaag atcgagctgg tgcaccgcct gcaggacgcc ggcctcactg
     1441 aaattgaagt caccagtttt gtgtccccca agtgggtgcc gcagatggcg gacgctgccg
     1501 aggtcatggc cggcatcacg cgcaagcctg gcgtgcgtta ttcggtgctg acgcccaaca
     1561 tgaagggttt cgaggccgcc gtgttgtcca aaccggatga gatcgtcgtg tttgccgcag
     1621 ccagcgaagc cttcagccag cgcaacatca attgctcgat tgaagaaagt attgaacgct
     1681 tccgcccggt cacagaagcc gccctgtcgc agggcattcg tgtgcgtggc gccatctctt
     1741 gcgccgtggg ctgcccttac gagggcgaag tggcgccgga gcgtgttgag atggtcgcgc
     1801 gtttgctcaa aggcatcggt gtgcagcaca tgggtgtggc cgacacgatt ggcgtgggca
     1861 cgccgctcaa ggttcagcgc gcgatggaag ccgcgctgaa acactacgcc atcgacgacg
     1921 tgtcgggcca tttccacgac acctatggcc aggctctggg caacacgctg atctgcctcg
     1981 aaatgggcat ctggaactac gacacctcgt cggccggcct gggcggctgc ccgtatgcca
     2041 agggtgcaac cggcaatgtg gccaccgagg acgtggttta catgctgcag ggcatgggca
     2101 tagacaccgg catcgacctc gacaagctga ttgatgccgg caagttcatc agcgactacc
     2161 tggagcgcaa acccaattcg cgtgcggcca ccgcgctgct gaacaagagg gccggctgat
     2221 gtgtggtgcc gagctggtgt ctttgcctga aggcgttcag cgcgttgccg ctgagctgca
     2281 ggccaagggc cacccgcatt cgccggtgat gctggacggc gcggcacgca ccgcgcagca
     2341 ggcggctgat gccctgggca tcgccctggg ccagatcgcc aaaagcatca tctttcgccg
     2401 caagtccgat gacgcggcgg tgctggtcat cacggccggt gaccagcggg tcgacgagcg
     2461 caaggtcgaa gccctggttt gccccgatgg caaacgtctg ggccgtgcgg atgcggagtt
     2521 tgtcaaaacc cgcaccggtt tttccatcgg tggtgtctcg ccgctggcgc atgccacacc
     2581 gcccgttacg ctgatcgacc agagcctgtt ccggtttgaa gaggtctggg ccgcggccgg
     2641 ccacccgcat ggcgtgttca gactgagccc gcaggagctc gtcactctca ccggcgcgcc
     2701 tgtggccgac gtggcggtgg acccggtgca ggagcaggtc gcgcagcaac gcgctatctt
     2761 tctggtagct gcccgcgccc gtgaaataag gggcgagacc gaaaaccttc cttcaccctg
     2821 catctcggta tgccgcatgg acgcggtccg cggctggtgt gaggggtgct ttcgcacccg
     2881 cgacgaaatt gccggctgga gcggggcgac ggatgcgggc aaacgggcgg tctggaccct
     2941 gatcgagcag cgcatggccg cgctgcaggc ctgacgggca ccgtcctccc tacatccatc
     3001 ccggcgagcg tgccatgaaa cacatcacct tctacctcga cttcatctcg ccctacgcct
     3061 ggctggcttt cgagaagctg cccgaggccc tgaagggtct cagctacagc gtgacctaca
     3121 agccgctgct gtttgccgga ctgctcaaac accacggcca ggccggcccc gcagaaattc
     3181 ccggcaagcg cgagtggacc taccgccagg tgttgtggct ggcccaccag cacggcatca
     3241 agctgcaact gcctgccagt catccgttca acccgctggg cctgctgcgc ctggccgtgg
     3301 cctgcgatgc acacggcacg cccaaccgtt atgtctgcga gaccctgttt cgtcatgtgt
     3361 ggcaggccgg gctggaggcg gccgatccgg agcggctcgc gctgttgacg gcgcagctgg
     3421 cgccgcaacg cgacatcgac ggcgcagacg tgaaagcgca gttgaaggcg cacagcgacg
     3481 aggccatcgc gctgggtgtg tttggcgtgc ccgcgctggc ggtggacgg
//
LOCUS       DAIDFG010000323         3489 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_323_length_3489_cov_15.973209, whole genome shotgun sequence.
ACCESSION   DAIDFG010000323 DAIDFG010000000
VERSION     DAIDFG010000323.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_323_length_3489_cov_15.973209"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          684 a         1028 c         1093 g          684 t
ORIGIN      
        1 cgctgcacgg cggcgggtat ggacgactac ctgaccaaac cgctgcatgt tgccgcgttg
       61 gcccaggcac tggagcgctg gctgcccatg atgacggccg ctgcgggagg ctctggtgga
      121 tttgcaacgg ctcccccggt gcggggccag gggatcgccg ccgggccgca ggctgtggcg
      181 ccgacgccaa aaagtggccc tgtgcctgcg gttgaacccg tgctgatgga cctggagcgc
      241 ctggcccagt tcaaggagtt cgacgacgac gcgctgacca tgacgcgcga ggtgatcggc
      301 ctgtttgtcg ccgacgcctc ccagcgcctg agcgccattg aagagagtat ccgtgccggc
      361 gacgctgccg ccctggcttg ggcgacccac gccctgatag gcgctaccag caacgtcggc
      421 gccgtggcca tgcaaaccgt gtgcagtgag ctggaactgg tggcaaaaac cggtgagatc
      481 ccggccaacg ccctccagca actggaaagg cttcagacct actgggccaa gaccagcgtc
      541 atccttgaca cttgggtctg aggctgccgt ctttttgcgg gtttcgggaa gttaggagag
      601 catatttcct ggcgtgagct tcgtccatga aaagtttgca cccacttact gatggtgaat
      661 tcgaccctgt ggtgtgctgg ggaaatcatc aaatccggtc agattcggtc tacggtcacc
      721 tcaagtgtca tctgcaattg tgactttgtt cacaaaatcg agactccata ttcactatcc
      781 agaatcgggc aggcttcata cgcttaacaa cctgtaacag ctaagccctg aagatgaaca
      841 ccctgttgac cgctggattc ctggatgctt ccctgccaca ggacgcgacc cgcccgcacg
      901 caggtccgga cgtgctgtcg ctgctggttg acgtactggg ccacggcgtc attgtggtgg
      961 gtgagcacgg ccagatcatc cacgccaatc gtgcggcccg caccgagctg aaccgtcatc
     1021 gggtgctcaa cagggtgggc ggggaagtcc atgccacgac gccggcagac tgcaaaacgc
     1081 tccagaatgc cttgggcaag gcggtggcag gcaagcgcag cctgatcaat gtgtctggcg
     1141 ccggactgac cctgacgctg gccgttgtgc ccctcaaacc cgacgccgat gcctgggatg
     1201 cccgcatcgc cctgcttttt gcgcgtgccg aagtctgcga atccggcatg ttcggttttt
     1261 ttgcgcgcag ttacgggctg acccagactg aagagcaggt gctcgccatc ttgtgccggg
     1321 gcctgagcac gccggaaatt gccgtccagc tgaaggtggc tgtttccacg gtacgcagcc
     1381 atgtgcgtag cctctgcgca aaaaccgggt ccagcggtgt gcgcgagctg gtgaaccgtg
     1441 tcgccgtttt gccaccggtg gcgcctttgc acctggccca aattcactga aagggcctgt
     1501 tgcccccaag cggcccacac ttgggggccg ccgcgtgcta gattacggct catcatgagc
     1561 acgtcaatac cttcaaacga agaccttttt acgccactgg ttcccgagtt gcgctcgctg
     1621 gctcgccgcg gcgtgttgcg gacctaccga aaaaatgcgg tgttgatcaa tgagggtgaa
     1681 tccggcgaca gcctgtttgt cctgctcaag ggaagtgtca aggtattcgc catggacgaa
     1741 agcggacgcg aaatcactta cgggacggtc aatgcgggcg actatttcgg tgagatgtcg
     1801 ctcgacggcg ggccccgatc tgcctcggtg atgacgctgg agccctgcac ttgcgcggtg
     1861 ctgagccggg cagatgtcag tgaacacctg gtggaagagc ctgaatttgc catcaatctc
     1921 gtggttcagg tgatacgccg ggcccgttcc gcgacagaga cggcgcggaa catggcgctg
     1981 ctcgacgtat acgggcgact ggttgccttg ctggaagagc gtcgggaaga caccggcgaa
     2041 gcacccggcg gcagcgccac gctggagtcc atcacccatc aggacatcgc caatcgcgtg
     2101 ggcgcctcgc gcgagatggt cagccggctg ctcaaagacc tcgaaaaagg gggctacatc
     2161 gaactgggca tccggcgcat cacgttgctc aagaaactgc ccagccgctg gtaggccggt
     2221 tcgggtggcc tatttcccgt atggcgacga gtcgctgcca atagcgcaaa tggctgttga
     2281 ccgcttgtgt gctcaagagg tcacggccgt gggccccgcc tatcgctgcg catgttttgc
     2341 agttccttcc aaattgctca ggaagctcgc cgacaaagcc ggggaggctg atcgccatgt
     2401 catccatggc cacatggaaa gtgcctcgcg ccttcgtacg cagcgcagcg catgggctcc
     2461 ccaggcgccg gcagcgtcac tgccgcaggc gcttcattcc gccccgtcct ggcggcgcag
     2521 tatttttgat gcaggcacgg acaccgtgct tccaggcaag cccgtacggg acgagggcga
     2581 caagccggtc aaggatcacg ccgccaacca ggcctatgac aatgccggca tcacgcttga
     2641 tttttacctg aaggtttttg gccggcattc gatcgacaac cgcagcatgc gggtcgagtc
     2701 tgcggtgcat ttcggaaaaa atttcggcaa tgcgatgtgg accggcgacc ggatggtcta
     2761 cggagatggc gaccagaacg tgagtggatt taccgctgca ctggacatca ttgcccatga
     2821 actcacgcac ggcgtcacgc agcacatgat cgctggcggg ctgggtgttg ttcgggtggc
     2881 tccgaaggat cgcgaattca aggagcagac acatgcgctc caggggcaga gcggcgcgct
     2941 caatgagtct ttttccgatg ttttcggcag catggtcaaa cagtggcacg cggggcagga
     3001 cgtcgcgcaa gccaactggt tgctcggtga gcacatgctt gcgccgcagc acggccgggc
     3061 catccgctct ctcaaggacc caggcaaccg ccagctgacg tggtacgaag acgagcagtt
     3121 caagaacatg gagcagtatg tcgatggcgc tgacgtgcat gacggctccg gcattcccaa
     3181 tcacgccttt tacctggcgg cccgaaaaat aggcgggtac tcctgggaaa aagccgggcc
     3241 gatctggtac gaggccttcg ctaaactcaa gcccaaggcc agttttcagg atgcagcgcg
     3301 agcaaccaca gacgtggcct cccagcgctt tggcgttaag tcaaaagaat gcaaggccgt
     3361 catgtcggcc tggcaggctg tcaaggtgat cacctgaacc cgggtgcaaa tgccttgctt
     3421 tgtgaggggt accgtgtaac gattcgtagt ggtcccgttg tgtgttgttg agagttctta
     3481 aagaaatgt
//
LOCUS       DAIDFG010000324         3464 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_324_length_3464_cov_17.339396, whole genome shotgun sequence.
ACCESSION   DAIDFG010000324 DAIDFG010000000
VERSION     DAIDFG010000324.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_324_length_3464_cov_17.339396"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          752 a         1098 c          961 g          653 t
ORIGIN      
        1 gaagtcccgt gaagatacgg gaaccctaaa tcgtaactag tggacctcga tccaccaaaa
       61 ccacaacccc cagccaaccg cgatccagat ggccaggatc atgacagcgc cccacaggct
      121 gcgcggcctg gcgccggatt gcctcatcca ttcatcacct tgctgccggc accggtccaa
      181 cacgttcgcc ggcgtaagcc ggattgccag ccagatcaaa ccaggcaata gcaacaggtc
      241 gtcaacatag cccagcaccg gaatgaaatc gggaatcagg tcgatggggc tgagcgcata
      301 ggccactaca aatacaccga tggccttggc ataccagggc gtttccgggt gcttgcaggc
      361 aaaccacagg gttatcgcgt cccgctttag ccgtttggcc caatcgagga ggagttgcaa
      421 aactttcatg aagatgtgtc gtgttgtgcc tgtgccgcca ttcaatgcga atggcgatgg
      481 tgggcatcgg gaaagtgcgc atggctgtgc gcaataggac tgtgccggtg tgagtgggtg
      541 tggcgtgttc cctccacaac cacttcgtca tgaccgtgat cgtgatgctc gtcaccggag
      601 ccgtgttcat gctcatggtt gtgttcaagc ggctcatggg tatgcgcatg ctggtggctt
      661 tcggtcaggt gcagccagac accccatgcc atcagcccgc ccgcaataag cagcggcggg
      721 gagattggct cccccaggat gacgatggcc agcgcggcgc ccacaaacgg cgcgatggaa
      781 aaatacgcac ccgtgcgcgc agtccccagg tgacgcaagc caaggacaaa cagcgccagg
      841 ctggccccat aacttgcaaa acccagcacg gcggccgcca gcacaacggg aacacccggc
      901 caggccgcac ccagcgtcaa cgcaagcagc aggttggtcg cgccggcggc caggcccttg
      961 accatggcga tgaacgatgc gtccgacagc gaaaccttgc gcgtcaggtt gttgtccacg
     1021 ccccaggcca ggcatgcgcc gaccacggcc aaggctggcc acagctcgcc aaaccgcgcc
     1081 tcccccggcc aggacaacac cacaccgccc gccacaatgg ccagcatgcc cagggcaatg
     1141 cgcctgtcaa agttttcccg gaacacaaac cacgcaatca atgcagtgag cacgccttcg
     1201 gcattgagca ataacgaagc gccggaagcc ggcatgccgg acaggcccac catcaacaac
     1261 acagggccga ccacgccacc ggccaatacg gcaccggcca gccatttcca ctcgccagga
     1321 ggtaattgaa cggatggcgc tttgcgcagc agacgcaaaa cgaacaggcc tatgcccgag
     1381 ccgaggtaca acaaggcggc caacagccag ggactggttt gcgccaataa caacttggcc
     1441 agcggggtac cggcaccgaa caaagtagcg gccaccaaag tggccagcac accgggctgt
     1501 gtgagcgatt tcaagagcag gcctcccggt tgtccaggtt gaggtcctct aatgcagcac
     1561 gcaatggcaa tacacaaagt gctggacaag ccctgaaggc gtgtaatggt cctccgtcac
     1621 atgctccagc agttcaaacg cgccgccaaa ctctgcatgc agcgcgtcag gtgcataacg
     1681 cacaacgggc aagccgctgc attgaagcgg gccattcggc ccaaaggcag ccacgatgac
     1741 ctggccaccg gggcgtactg cgtggcgcac ctgggccaca taggccgcgc ggtcgaccgc
     1801 actggtgagg aagtgaaaaa cggcccggtc atgccagacg tcaatactgg atttttccag
     1861 gccgacggtg gtgatgtcac cttcgatcca gcgcacgcgc ctcgcctggt cacccaacct
     1921 gtggcgcgcc acattgagcg ctgcgccgga gatatccagc acactcacat tcgtcatgcc
     1981 gtcagccagc aggttgtcta ccaggctggc ggctccgccg ccgacatcca caatacgcgc
     2041 cgatgtctcc ggcgccacgc ggcgaatcag gtccagcgac aaagtggcat gaggctggta
     2101 ccagctcatg gtttcggacg atttggtctt gtagatcttt tcccaatgcg atttagcttc
     2161 catgaagcgc ctcccggtcg cggtgtaaat atttcatgat cgtcagatat cgtggcagca
     2221 cgatgccgta ctgcgccgct cagtgagcat catggagtgc cgaaatcaag gggcaggaaa
     2281 tgcttcccct tgccgcgcgg cagttctcca ccagagtgct cagaaccgac tctatgcggc
     2341 gcagatcccc cagcttttcg cgaacgtcca ccagtttgtg ttcggccaag cctctggctt
     2401 cttgacaatg cgtgccatcc tccagcctca gcagctccgc aatttcgtcc agactaaagc
     2461 caaggcgctg ggcagatttc acaaacctga cacggcttac ctccgcgtcc ccataacgcc
     2521 gaatactgcc gtaggggcgc tccggttcag tcagcaatcc tttgcgctgg tagaaccgga
     2581 tggtctcgac attgacgccc gcggaagcgg caaactcgcc aattgtcaga tagctcacga
     2641 cgtctttcat gtccttgact ccgtacttga gtacggaagt aatcttacgc catgaaatcc
     2701 agccttgcta taaaccagca cccccccgca caacagagcg gcaccagcac gctggttgtc
     2761 ggcggtcttg ccgcctttct cgcatccgcc tgctgcctcg gcccattggt gctgattctg
     2821 ctgggtttta gtggcgcctg gatcagcaat ttggctctcc tggagcctta tcgccccttc
     2881 tttatcggaa ttgctttact ggctttgtgt ctggccggac ggcgcctgta tcgagccacg
     2941 aaggcctgca cctcgggtga agtctgtgct ttaccgcgag ttcgcattct ctacaaagtc
     3001 actttttgga cagctgcggc tttggcgctg gtcgcgcttg cgttccccta cgtcttgccc
     3061 ttgttctact gaaaaggaac tacccatgaa gcgccgtatc ccgccgctat tcgcattggc
     3121 cgctgccttt gtcctccctg catgggctgc gcccaagatg gtgactttgt cggtccccgg
     3181 catgacttgt gccgcctgcc cgatcacagt gaaggtggcg ttgactaaaa ttcctggcgt
     3241 ctcgaaagct gatgtcagtt acgaaaaacg gcaggcaacc gtctttttcg atgatgcaaa
     3301 aacaaactct gcggcgctca cccgggtcac cgcagacgcc ggctatccct cgcatgtggt
     3361 cggggtggca ccatgaaaca ggtcatcctg gattcgaccc tgacatgccc gagttgtggc
     3421 cacaagaaaa cagaaagaat gcctgaagac gcgtgccaat ggtt
//
LOCUS       DAIDFG010000325         3446 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_325_length_3446_cov_19.161899, whole genome shotgun sequence.
ACCESSION   DAIDFG010000325 DAIDFG010000000
VERSION     DAIDFG010000325.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_325_length_3446_cov_19.161899"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          584 a         1085 c         1069 g          708 t
ORIGIN      
        1 gcccagcttg tggttcaggc cggccagcgc cacgtcattg cgttcgccgg tcagctggcc
       61 atcggtgccc accattttct tgggcacgat gaacagcgag atgccgcgtg tgccgggaat
      121 cagcttgccg tccggcccgg ggatcttggc cagcaccagg tggatgatgt tttcggtgag
      181 ctcgtgttca ccgctcgaga tccacatctt gttgccggta agccggtagc gcgggcccag
      241 cgcctcggct tcgaagttgt caccgtccgg cacggcgcgt gtcgtgatgt cgctcagcga
      301 ggaaccggcc tgcggctcgg acaggcacat ggtgccggaa aaacgcccgg aaaactcgtt
      361 gcgtgcaaac acctctttct gcgcttcggt gccgtgcacc agcagcaggt tggcgttgcc
      421 cttggtcagc aggctggagc ctatgctgac cgaggccatg gcgaaaaagc agttggccgc
      481 cgcctcgatg gtgtaaggca gctgcatgcc gccttcgtcg taatcctgcg cggcgctgag
      541 catgccggag ccggcgtagg ctttgtgcgc gtcatgtgtg gcctgcggca gaatgacttt
      601 ttcgccgtcg aaatgcggct cctgggtatc gaccagccgg ttgaaaggcg caaatttttc
      661 ccgcgcgata cgttcgcagg tgtcgaacac cgcgtcgaag gtttcgcgcg aatggtcgga
      721 aaaacgctcg cgctggttca gcgactcggc gcccagccac tggtagagca ggaaatcaag
      781 ggtgggtctc aggctcatgg gtatccgatc tggaggaagt tcaggtgacg ctgcttttgc
      841 ctggcagccg gacgatcgtg ccttgggcca cggcgcagag tttttcctgg ccgcccacca
      901 ccacaaacac gtcgcagcgg cacactgcct gcgatttgcc gacatgcacc gcctcggcgc
      961 gcgcaatcag gcgctcgccg accgcgggcc gcacatagtt gatcttgaat tcggaggtga
     1021 cgacggccca gtccagagtc gtgccgccgg cataggtcag cgcgttgtcg gccgcgtaac
     1081 tgaccacgcc gccgtgcaca aaaccgtttt gctgaaggat gttcggcgct atcggcacct
     1141 gaagttcgca gcgcccgacg gacaaggcat gcagctcggc gcctattaac acactgaaag
     1201 gctgcagggc cagaaacgcc tgccctttct gaaggagcag ggtttcatcc atgcgttgcc
     1261 cgcctcttac aggacttcga aaatgccggc cgcacccatg ccgccgccaa tacacatggt
     1321 cacgcagaca cgtttggcac cgcggcgctt gccttcgatc agcgcgtggc cggtcaggcg
     1381 ctggccgctg acgccgtagg ggtggccgac cgcaatcgcg ccgccgttga cgttgagctt
     1441 gtccatcgga atgcccagct tgtcgcggca ataaatcacc tgcacggcaa acgcttcgtt
     1501 gagctcccac aggtcgatgt cgctgacctt caggcccagg cgcgccagca ccttggggat
     1561 ggcgtagacc gggccgatgc ccatttcgtc aggctcgcag ccggcaaccg caaaaccgag
     1621 gaagcggccc agcggtgcca ggcctttttt ctcggccact ttttcatcca tcaccaccac
     1681 agcgccgccg ccgtcggaga actggcttgc attgccggcc gagatcacgc cgccgggaac
     1741 ggcgggcttg atgccgctga tgccttcctt ggtcgtgcct tcgcgaatgc cttcatcctt
     1801 gctgaccgtc acctgcttgg tggtcaggcc catcacggcg tcggccacac ccatggtgac
     1861 ggtgatcggt gcaatttccg cgtcgaacag gccggcagcc agggctgccg aggctttttg
     1921 ctggctggcg gcgccgtatt cgtccatctg ctcgcgcgag atgccgtaac gcttggcgac
     1981 ctgctcggcc gtctgcagca tgttccagta aatctcgggc ttgttcttga ccagccacgg
     2041 gtcggccagc atgtgggtgt tcatttcctg ctgcacgcag gaaatgctct caacgccacc
     2101 ggccacataa atgtcggctt caccggcaat gatgcgctgc gcggccaggg caatggtctg
     2161 caggccggac gagcagaaac ggttgacggt catgcccgac acggtgatgg ggcagccggc
     2221 gcgcagcgcg atctggcgcg caatgttggc gcccgtcgcg ccttcggggt tggcgcagcc
     2281 catgatcacg tcctcgacct cgccggcctc gatgccggcg cgctggatcg cgtgttcaac
     2341 cgcgtggccg cccagggtgg ccccatgggt catgttgaag gagcccttcc agcttttggc
     2401 caggggggtg cgggcggtgg aaacgattac agctgaagtc atgaaaatct ccgtgaatta
     2461 attgaacgtc ttgccttcgg cggccagctt ggccagcagc ggtgccggtt gccagaactt
     2521 ggcgtcgtcc agtggattct gggcaaaacg cttcatgctc tgcaccacgt tgaacaggcc
     2581 cacctggtcg gcgtaattca tggggccgcc gcggtagatc gggaagccgt aacccatgag
     2641 gtagaccatg tcgatgtcgc tggccctgga cgcaatgccc tcttccagaa tgtgcgccgc
     2701 ctcgttgacc agcgcgtaaa ccaggcgctg cacgatttcc tcgtcggaaa tcttgcgtgg
     2761 cgtgatgccc agcgatgcgc ggtggtcttc aatcatcttg accacttcgg cattcggaat
     2821 cgcatcgcgc ttgccgggca cgtagtcgta ccaacccgca ccggttttct ggccgaagcg
     2881 gcccttctcg cacagcagat cggcggtctt gctgtatttc atgtccggct tttcctggta
     2941 gcggcgtttg cggatggccc agccgatgtc gttgccggcc agatcgccca ttcggaacgg
     3001 gcccatggcc atgccgaatt tctccatggc tttgtcgacc tgcgcgggcg tgcagccttc
     3061 gtcgagcagg aagccgccct ggcggccgta ctgctcgatc atgcggttgc cgatgaagcc
     3121 gtcgcagacg ccggacacca cggcggtttt cctgatcttc ttgctgacag ccatcaccgt
     3181 agccagtacg tccttggcgg tcttctcacc gcgaatgact tcaagcagct tcatcacatt
     3241 ggccgggctg aagaaatgca tgccgaccac gtcctgggga cgcttggtga aggccgcgat
     3301 cttgttgacg tccagcgtcg aagtgttgga ggccaggatg gcgccgggct tcatcacgcg
     3361 gtcgagttcc ttgaatactt tttccttgac gcccatttcc tcgaacacgg cctcgatcac
     3421 catgtccgcg tctttcagct cgtcgt
//
LOCUS       DAIDFG010000326         3436 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_326_length_3436_cov_16.713990, whole genome shotgun sequence.
ACCESSION   DAIDFG010000326 DAIDFG010000000
VERSION     DAIDFG010000326.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_326_length_3436_cov_16.713990"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          605 a         1137 c         1146 g          548 t
ORIGIN      
        1 gcgcagcgcc ccctcggggg gcagcgcagt acgcgaagcg acaagcgtgg gggcctgagg
       61 cgtgtgcctg atcgcctttg ccattggtgc ttcaccgcgc tggccgctgg tcatcgccgc
      121 gaatcgcgat gaattcctga accggcccac ggcgccgctg gcacgctgga ccaccgatgc
      181 gggcaaggaa gtgatctccg ggcgcgacct gcgcgccggc ggaacctggc tgggcctgag
      241 ccccggcggg cgggtggcga tgctgaccaa tgtgcgcgag ccgcacccgg tcgccggtga
      301 gcggtcccgc ggtgagctgg tcatgcgctg gctcgaaggc gaaatggatg ccggccagtt
      361 catggcgcaa accgacagcg cggtctatgg cgccttcaac ctggtgctcg gtgacttcca
      421 gtcctccgcc tggcactggc tcagcaacca gtccgacccc gacggtggcc cggtcaaggg
      481 ctggcaggcg tgcgcactca agcccggtgt ttacggcctg tccaacggcg cgctggacac
      541 gccctggccc aaaacccgcg cgctgaaaaa agcgttgcac caggcgctgg attccgacag
      601 gcaagaggac ctgcaggcag gactctggtc cgccctgcaa gacaggaagc gccccagcga
      661 cgggcacctg ccgcgcacgg gtgtgccgct ggccctggag cgcgcgctgt cgagtgtgtt
      721 tgtcgatttc cccgagcacg gttatggcac gcgtagcagc acgctgctgg tggcgcgtgc
      781 tgaggaaagt gcttctcctg cagttacatg ggccgtcgag gtcgatgaaa tcacccacac
      841 acacggccct cggcatggcg cgcagcgggg ggcgggcagc cgctgccacc tgatctggca
      901 gcagggcggg acctcgccgt aaattcaggc ggcggtattt ctaaaggggc ttgaccatct
      961 cgatgtgggc aatgtccacc tcatcgaagg gctcgccgcg cgcggtgaaa cccagccggc
     1021 tgtaaaaccc ttcggcgctg cgctgggcgt gcagcagcac ctcggtgtcg ccgcgttgcc
     1081 gggcctgggc catcagggcg tgcagcaggt cgcgtcccag gcgggtgccg cgcagcaccc
     1141 ggctcaccgc catgcgcccg atttttccgg tgccggggac gtgttgcagc agccggcctg
     1201 tggccaccgg ctggcccagg ccgttgtagg ccacggcatg cacggcgctg tggtcggctt
     1261 cgtcccattc catctcgagc ggaatgccct gctcttctat gaacacttcg gagcggatgg
     1321 cacccgcatg ggtgccgagt tcggcccagg agcccaactt gagcgtgacc atgggctcgc
     1381 cggcctcgta ggcggtcaaa atgcggcgca agccgtcggg caccggccgg gaagtttgcg
     1441 tgaccgggtc ggcaaacacg tagatcaact cgccggtgat cagcaactgc tcgccacgga
     1501 aaatggcacc ggtgaacacc atggacgagg tgccgatgcg tgagcacctc atggcaacgt
     1561 cgagctggtc gtcgcaatgc gccgaagcat ggaattcgac ggtcgctttc ttgacataca
     1621 gctcgcctcc cagctggtgc atggcctgtt catacggcag ggccagcgcg cgccagtagt
     1681 cggtgacggc cgtgtcgaag tacatcaggt agtgcgcgtt gaagacaatt ttctgcatgt
     1741 ccacttcggc ccagcgcacg cgcaggcggt gaaaaaagcg gaagtcctgt cgtttcatga
     1801 gtcagtctct ttcaaaaaag cacggcgcaa ggcagcggcc gcgtcggcat gggcttgccg
     1861 cgcctcggga ataatgcggc ccatcttgat gaattcgtgg gtgacgccgc ggtagatttc
     1921 cagatcgacc gccacgccgg ccgctctcag cttgtcggcg tacatcacgc cttcgtcgac
     1981 cagcgggtcg cattctgcca gccccaccca ggccggcgcc acgccgtcca catcgggggt
     2041 gttcaggggc gcgaaacgcc agtcgtcgcg gtcggccggg ctgcgcacat aacggctgaa
     2101 aaaccagttg atctcgcgct cctcgagcac aaagccgcgt gcaaactggc ggtgtgaggg
     2161 ggtgtcctgg tgtgctgaac agccggggta aaagagcagt tgcagggcca gcggcaggcc
     2221 ggcatcacgg gccagcaccg cacacaccgc agccagggtg ccgcctgcgc tgtccccgcc
     2281 cacagccaac cgctcactgt ccagacccag cccgtttgca tgggttgcca accactgcag
     2341 cgcatcctca gcgtcatggg cggccgtcgg gaacttgtgc tcgggggcga ggcgatagtc
     2401 caccgacacc accgcgcaac ccgcgaggtg gctgagctgg cggcacaaaa tgtcatgcgt
     2461 cgcgatgctg cctatggtga acccgccgcc gtggaaatac agcagcacag gcagcttgtc
     2521 ggtcgagggc gcgtacagcc gggccggcaa ggcatggccg tcgcgcgctg gaatgccgaa
     2581 gtcctcgacg cgcgccagtg ccggtttggg aatttccagc acgccggcgc cggcttcgta
     2641 ggccgcgcgg gcctgggtgg ccgtcagtgt gtgaaacgcc gggcgcccgg cccgccccat
     2701 gcggctgatc acgtcgcgca tggcgggcgt cagcagggcg tgtgcggcct gtccccgcat
     2761 ttaccgggaa accagaaaca tcggccgggc gcccgctatc gccaaagtga ccatgatccg
     2821 ggtatgcatg caagtcaaga gagtctccag ccggtaaagt agcgtgttca ggttggggca
     2881 ggaatacgcc aaccgccccg catcgtacaa cgccgaaaga cagcaaacca tggccttcat
     2941 ttactacgtc acccagatcc agttcgacta cggcgcggtc aagctcctca agcaggaatg
     3001 tgaacgtgtg ggcatcagca agccgctggt catcaccgat gccggcgtca aggcggccgg
     3061 cgtcctgcaa aaagcgctcg acgccctgcc cggcatgacg gtggcggtgt tcgaccagac
     3121 gccctcgaac ccgaccgagg ccgccgtgcg cgccgcagtc gccgtgtaca aggcccaggg
     3181 ctgcgacggc ctgatcgccg tcggcggcgg ctcggcgatt gactgcgcca agggggtggc
     3241 gattgccgcc acccacgaag gcccgctcaa gacctacgcc accatcgagg gcggctcgct
     3301 gaaaatcacc gaccgcgcgg tgccgctgat tgccgtgccc accaccagcg gcaccggcag
     3361 cgaagtggcg cgcggtgcca tcatcatcct ggacgaccac cgcaaggtcg gcttccactc
     3421 ctggcacatc gttccc
//
LOCUS       DAIDFG010000327         3435 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_327_length_3435_cov_15.081657, whole genome shotgun sequence.
ACCESSION   DAIDFG010000327 DAIDFG010000000
VERSION     DAIDFG010000327.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_327_length_3435_cov_15.081657"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          578 a         1064 c         1116 g          677 t
ORIGIN      
        1 aagcacccag agccgccagg ccctggtgcg cctcgaccgg aaagtggtgt gccttgtccc
       61 aggccgcggc gtgcggccag agttcggtct gggtgaagtc gctcaccgcg tcgcggatca
      121 tttcctggtc ttgcgtgagc agcatgagag gtcctaccag gccaacgcct ggccgtcgtg
      181 gttgatgaaa gtgccattgt gttccgcgcg caggctggct acggtggtgc gcatatcgga
      241 aacgctttgc gtgacatcca gatcggcgcc agcgccaccc atgtcggtct tcacccagcc
      301 ggggtgcatc gcaacgcaga tggctttgcc ctgcaagcca aacgacatgt ccttgacgac
      361 ggagttgacg gcggacttgg aggcgcggta cagccagctg ttgccattgg tgcgcagccc
      421 tatcgagccc atccgggatg acaggaccac cagccgggcg tcaggcgcca acacttccgt
      481 cagttgcggc agcacgcgca taggcgccag cacattggtg cgcatcacct gatcgaacac
      541 atcggcagcc ggtgggtcgc ccgcattgcc gcccgccagc acaccggcga catacagcgc
      601 gacgtcgatt ttttcgccat cgagtaacca ggccagaccg ctgacgctgg atacgtcgtc
      661 cacctccagc ttgaccgcct gcgcgcccag cgccttaagg cgctccagcc ccgctgcgtc
      721 gcgcgcggtg gcgatcacgc gcgcgccggc gctccggtac tggcgcacaa actccagccc
      781 gatgccgcgc gaggcgccga tgacgagtac cgtgctcatg tcagattttc atgcatcaaa
      841 tcggcctcca gaccttatac agcgtgcgtc aacagctatg taatttatag caattcaatg
      901 gcaattgccg tgccttcacc gccaccgata cacagcgtgg caacaccttt tttcaggccg
      961 cgggccttga gcgcgtacat cagcgtgacg atgatgcggg cgccgctgca accgatcggg
     1021 tggcccagtg cgcaggcgcc gccgttgacg ttgacgatgt cgtggctgat gccgagctct
     1081 ttcatggcgg ccatgggcac cacggcaaaa gcctcgttga tttcccacag gtccacgtct
     1141 ttcacgctcc agccgatttt cttgagcagc ttctgcacgg cgccgaccgg tgcggtggtg
     1201 aaccactcgg gcgcctgggc aaaggtggcg tggccgacga tgcgggccag cggcttggcg
     1261 cccagctcct tggccgtcga tgcgcgggtg agcaccaggg cggccgcgcc gtcgttgatc
     1321 gacgagctgg acgcggcggt gatggtgccg tctttcttga aggcggggcg cagtgtggag
     1381 atcttgtcga gtttgacctt gcccggacct tcgtcgatgg aaatcacggt gtcgccgctg
     1441 cggcccttga cggtgacggg ggtgatttcg gccttgaagg cgccggactc ggtggcggcc
     1501 ttggcgcggt tcacgctggt ggtggcgaac gcgtcctgcg cctcacgcgt gaactggtac
     1561 ttctcggcgc actgctcacc gaaggtgccc atggcgcggc cgggttcgta ggcgtcttcc
     1621 aggccgtcga gcatcatgtg gtcgtaaatg cggtcatggc cgatgcggat accgctgcgg
     1681 cccttgagca gcaggtgcgg tgcgttggtc atgctttcca tgccgccgga gaccatcacg
     1741 tcgcggctgc cgaccagcag ctggtcgtgc gcaagcatgg tggcttccat gccggagccg
     1801 cacatcttgg acaaggtgac cgcgcccgcg ctggcgggca ggccaccctt gaagccggcc
     1861 tggcgcgccg gcgcctggcc ctggccggcc atcaggcagt tgccaaacag cacttcgtcg
     1921 actttttcgg gcgcgacgcc cgagcgttcg atggcggcct tgatggcggc gccgccgagg
     1981 tcgtgcgcgg cgaggctgga aaagtcgccc tgaaagccgc ccatgggggt gcgggcggcg
     2041 ccgagaatga cgatggaatc agtggtggaa ctggacatgg tggttttctc ctgagttgaa
     2101 caagttaaac aaattaggca agctggatga ctgcgccgcg ctcttcgcgg gcgatgcgca
     2161 ggatgtattg ctgaaagccc gggtcttcac cgcgcagcaa cagcggcagg gcattgaaaa
     2221 aattctcgcg ctggcaccac tcgcgcatgt aatcgtccca gctgttccag cgacgcgcct
     2281 cgacctcgct catgcgttcg ctccaggcga cgatgtaggc gcgctcgaac agcgaaatca
     2341 gcatgtcgta gatgatcatg gtgcgttcat tctgctcgcg cgtgggactc tccagcggct
     2401 cgttggtgcg caggtgcagg tcggggttgg ccagcacgac tttcagaaag tcgttgtagg
     2461 cgtcggacag cagctggtag gccgcctctt cctcgttgtt gcgctccttg cgctgctcga
     2521 gggcaaagaa aaatatcgca aacggcaggg ccagcgcggt gaccacgaag ctggcgtatt
     2581 cccaggcgtc ccgcatggcc gtcctcaggc tttctgcgcc gcgtgcctgc gcacaaaacg
     2641 ccgctcgcgc tcgtagggga acacatcgtg cacatggccg gcggcaatac gctccttgtg
     2701 gccctgccag aaggcggcat cgagcaggtc ggcgtggtgc ttcatgaaga cctcgcgcac
     2761 cgcggcgttg ccgagcagga agggctcgaa ggtctcgggg aacacgtccc ggggcccgac
     2821 cgagtaccag acctcgccgc tcatttcttc ttcctcgttg cgcggcgtgg gcaccttgcg
     2881 gaagttgcag tcggtgaggt attcgatctc gtcgtagtcg taaaatacga ccttgccgtg
     2941 gcgcgtgatg ccgaagttct tgaacagcat gtcgccggga aagatgttgg ccgccaccat
     3001 gtccttgatg gcgttgccgt attcgaccac ggcgcgctcg atctgcgcct tcgccgccgg
     3061 ctcgtccacg ccggtgtcga aggcttcctg caggtagatg ttgagcggga tcatgcggcg
     3121 ctcgatgtac acatgcttga tgatgacctc gatgttgccg tcgccgtcgc ggtcgctgat
     3181 ctcgagctgg ctgggtgcaa atttctcgat ctcggcgatc agttcgtcgt caaaacgctc
     3241 acgcgggaag gccacctcgc tgtactcctg cgtgtcggcc atgcggccga cacggtcgtg
     3301 ctgcttgacc agcaggtact tggccttgat ctgctcgcgc gtggtttcct tctgcggcgg
     3361 gtagtagtcc ttgatgacct tgaacacata gggaaaggac ggcagatcga ataccagcat
     3421 gaccatgccc ttgat
//
LOCUS       DAIDFG010000328         3418 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_328_length_3418_cov_17.174547, whole genome shotgun sequence.
ACCESSION   DAIDFG010000328 DAIDFG010000000
VERSION     DAIDFG010000328.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_328_length_3418_cov_17.174547"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          750 a         1035 c          974 g          659 t
ORIGIN      
        1 cttcacggga cttcaaataa tcgatagaat cgaactccga tgtcgcgttt tcgccacttt
       61 ttgctttgct tgatgatgct ggctttgccc ctgcagggct ttgctgcggc ctccatgctg
      121 tattgcggca tggggccaga ccacggtgag caggtatctg tagcggcgag ctaccatcac
      181 atggatggct taggcgaggt gcagcacgag cattcatcca tggggaaaac ggaaccgctt
      241 gcaaagcagt ccccggatgg tcaaaagcag cttcccaata ccttccataa gtgcggcgtt
      301 tgtgcttctt gctgcagtgt cctggcgatt tctgattttc cccggaccgt caaggttcaa
      361 tcttctcccc atgctgatct ggccgagctt agcgtcctga ttcacgccgt accgccgggt
      421 ctccccgaaa aacctcctca cgtctgacgt gcgcccgatt gctgcggccg ttcgtgccgc
      481 agcgactggc gttcttttca ttactcgcca tgaaagtatc cctccaggga tgacttttct
      541 gtcgccgtct catgaaaacg cgaggaatcc atgtttaaaa actcacccgc catttggctg
      601 gccccgatat ttttcctgtc ggggatagct gtcgcacaaa cgtcagcgcc agcatcccca
      661 atccaggagg cgccccaagc cacgcccccc gtctaccgtt cggcctttga gggttacaag
      721 ccctataccg acgacaagct cctcaactgg aaggaggcca acgacaacac cggccgcatt
      781 ggtggctggc gtacgtatgc aaaagagtcc agacaacccg acgacaaggc gcccgctcac
      841 gacatgagca agatgcccga accgaaaacc ggaggcggca agccatgaaa gcgccatccg
      901 tgaaaaccat tttcaagtct ggtgcagtgg ctgctgcggt tctggtgctg gcgggatgtg
      961 cctctgtaaa cattgatcaa acggtcaaag acaccaatga cacaaccggg gcctttaccc
     1021 agggcaagct ggaactcagc cgcactgacc agcaactgca agcccgcaag aaactaagcg
     1081 aggagctgct ggccaagccc ttaggcatgg acgaggcggt ccagcttgct ttggcgaata
     1141 gcccggcagt ccagactctt ttggcccaaa gctggtccgt gatggcgcag gccaaccagg
     1201 cgggacgcat tgccaatcca atcttttcgt tcgaacggat tcgtttcggc agtgagttgg
     1261 agatcggtcg cctgctgtcc tttggtttgc ttgatgtctt gaccctgccg caacgccagg
     1321 ggatttcccg cggactgatc gcacaggcca gggttcaact cagcgccagc gtgatcgatc
     1381 aagtcaccca agtgcggcaa gactgggtgc gagccgtcgc tgcgcagcag acgctgcaat
     1441 atgcagaaca ggtcaacagc agcgccaagg ccagcgcaga actggctcaa cggatgcagc
     1501 aagttggcaa cttcaccaaa ctgcagcgcg cacgccagca ggtgttttac gcggatagcg
     1561 tggcacagct ggcctctgcc cgccatgccg caaccgctgc gcgcgaggag ctgactcggc
     1621 agcttggcct gacagacgcc caggcggggc aattgcagct cccggagcgt cttccggatt
     1681 tgccgaaaga gccacgcgca gctgacgccg tctcggcgaa ggccacggag caacgcctgg
     1741 atgtacaaat ggcgcgcagc cagctggaca ccgccggcag agcccagggc ttgaatttgc
     1801 tgaccagtct ggtcgatgtg gagttcggga tacggcgcga taccgtgttt gacaaccacg
     1861 acggaactaa aacgccacgc aaaggctatg agcttgacat ccgattgcca attttcgact
     1921 ggggaaccgc tcaacgggat gccatgaacg cgcaatccct ggctgcagcc aatcgctacg
     1981 agggcacgat ccgaaacgca gcatcacaac tgcgcgagag ctattcagcg tacaggacgg
     2041 cttacgacgt ggccaagcac tatcgggacg agatcgttcc gctgcgcagg gccatggctg
     2101 aagaaaacgg gctgcgctac aacggcatgc tgattggtgt gtttgaactg ctggccgaaa
     2161 gccgcgacca gatcgccagc gtcaccgcgg ccatcaattc gtaccagcag ttctggctgt
     2221 cagacgccgc gctcgcagcg tccgtgactg gcaggccgac ctccatgatg atggccgcgc
     2281 ccgcggccgg tggtgccagc gccgccgctg gtcattgacc gtagcccaat ttacccaacg
     2341 gattacatat gaataacaga agaaattttc tgactggtgc tggtgcgttg accactgcgg
     2401 ttgccgccgc tgccgtcagc aaagtcgcca tggccgcgct gcccgagccg gtcattcaga
     2461 ccaaacccga caccatggcc ccgctggtgc cgaataccgg ccggccgtac aaccccgtcg
     2521 tcaccctcaa cggctggacg ctgccttggc gcatgaacaa tggcgtcaag gagtttcacc
     2581 tggtggccga acccgtggtg cgtgaaatgt cgcccggctt caaggcccac ctgtggggtt
     2641 ataacggcca gtcgccaggg ccaaccatcg aagtggtcga aggcgacaga gtccgcatct
     2701 tcgtgaccaa caaactgccc gagcacacca gtgtccactg gcatggtcag cgtttgccca
     2761 acgggatgga cggcgtcgcg ggcttgaacc aacctgcgat tccggcaggc aagacctttg
     2821 tctatgaatt tgtggcaagg cgtcccggca cgttcatgta tcacccgcat gccgacgaga
     2881 tgacacagat ggccatgggc atgatgggtt tctggatcac gcatccgaaa gccgcgcacc
     2941 ccctgatcga cgaggtgaac cgcgactttg tatttctgct caatgcctat gacatcgacc
     3001 cgggcctgta cacgccaaaa atcatgacca tgctcgactt caacctctgg agctggaaca
     3061 gtcgggtctt cccgggcatt gatccgctgg tggttcgcaa aaacgacaag gtgcgcatcc
     3121 gtatcggcaa cctgaccatg accaaccatc cgatgcattt gcatgggcat gagtttttgg
     3181 tgaccgggac ggacggcggg ccaacaccca aatcgacgcg ctggcctgaa gtgacaaccg
     3241 acgtggcagt cgggcagatg cggcaaattg agttcctggc cgacgaagaa ggtgattggg
     3301 ctttccactg ccacaagagc catcacacca tgaacgccat gggccacgat gtaccgacca
     3361 tgattggcgt cgatcatcgg aaagttgcca gtgagatcac caaattggtg ccggacta
//
LOCUS       DAIDFG010000329         3402 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_329_length_3402_cov_16.792949, whole genome shotgun sequence.
ACCESSION   DAIDFG010000329 DAIDFG010000000
VERSION     DAIDFG010000329.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_329_length_3402_cov_16.792949"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          663 a         1078 c         1085 g          576 t
ORIGIN      
        1 actcgacgct gtcggtgcgc accatgctcg agggcgcggt gacggccggg gtggtgctcc
       61 tgatcacgct gtggatttcc gcggcgattg aaacgcgcct gttgcgctcg gctaccggca
      121 acgagctgtc gctgcgcaag gccatcagca acgccacccg cgcgatgctg atgtttgtcg
      181 gtctgctgat ggcgctgtcg gcggtgggca tcgacctgac ggccctgtcg gtgctgggcg
      241 gagcgatcgg cgtgggtata ggcttcggcc tgcagaaact ggccgccaat tacgtcagcg
      301 gttttgtgat gctggccgaa cgcagcgtgc gcatcggcga caacgtgcgg gtcgacaact
      361 tcgagggccg cgtcacggac atcaacgcgc gttacacagt ggtgcggtcg ctgggcggcc
      421 gcgagtccat cgtgcccaac gagatgttca tcagcaaccg gattgaaaac ctctccctgg
      481 ccgatccgcg cgtgctgcag tccaccgtgg tgtcggtggg ctacgacagc gacgtcgaac
      541 tggtgatgcg tttgctgacc gaggcggcgc tcgcccagga ccgggtgctg cgcgacccgg
      601 gcccgggtgt caacctgacc aacttcggtg ccgacggcct ggagttcacg ctgaactact
      661 ggatgacgga tcccgagaac ggccagcaga acctgcgttc ccgcatcaat ctcgccatcc
      721 tgcagtccct gcgcgacaac ggcatcgaga tcccttaccc gcagcgggtg attcacacgc
      781 gggccctgcc ggtgcaagac gtcaagactc cctgaggtct cccgtgccgc gccggtcccg
      841 gcgcggcgct tgcctttatt tcatgaaaga gggcagccag aggctgagcc cggggaaggc
      901 cagcaggatg cccagcgcca ccacctgcat gaccacaaag ggccagacgc cgcggaagat
      961 gtggctcagt ccgaccttgg gcagcagggt gttgagcaca aacaggttca tgcccacggg
     1021 cggcgagatc aggccgatct ggatcaccat cacgatcagc acgccgaacc agaccggatc
     1081 gaaccccagc gacaccacaa tcgggaaaaa cagcggaatg gtcagcagca ccatggtgag
     1141 ttcttccatc acggtgccca gcagcacgta aatcatcatc atcgcggcga cgatcatcac
     1201 cggcgacagg cccaggtggg tgatccactc cttgaggtca ttgggcatgg aggtgaagtt
     1261 catgaagttg gcaaaaatgg tcgcagcgat cagcaatgtg aacaacatgg ccgtggtgcg
     1321 cgccgattcc accagcactt cgaacgtggc tttgaccgtc aaccggcgcc gcgccagtgc
     1381 aaacaggaag gcgcccgccg caccgatgcc cgcgccctcg gtggcggtga acacaccgcc
     1441 ataaatgccg cccagcacca gcaccaccag caccagcacc ccccagatgc cgcgcaaggc
     1501 cttccagcgc tcggaccagc tcgacttttc ggcggccggt gcatgctccg ggtccatgaa
     1561 ggtcatgaaa accacggcca ccatcagcag cagggcgatc aggatgcccg gaatcacgcc
     1621 ggccgcaaac agctttccga tgttggtttc ggtcacgatg ccatagatca ccaggatggt
     1681 cgaaggcgga atcatgatgc ccagcgtgcc gccggcggca atcacgccgg tcgagagcga
     1741 gtcgctgtag cccagccgtt tcattgacgg gtaggcgacc ttgctcatgg tggcggcggt
     1801 tgcaatcgac gagccgcaga tcgcaccaaa accggcgcag gccgcgatgg tggcgtgtgc
     1861 cagcccgccg cgcaggtggc cgatgaaggc gtacgcggca tgaaacagct catgggccaa
     1921 cccggcgcgc gcgacaaaat tgcccatcag gatgaacagc gggatcaccg acagcgtgta
     1981 ggcaaaaccg gtttcatgga cgacctgggc cgtgctggcg agagccgggt accagccgcg
     2041 cgtcaggccc atgccggcaa tgccgaccag ccccatcgca aaagccagcg gcaggcgcag
     2101 caacgccagc acaaaaatgg cggcaaaacc aagcagggct tcggtcacag cgtggctcct
     2161 tcacccatgt cggcatcgga ggccgggcga aacgccatgc ccaggtggat cacgccggcc
     2221 acggcgcaca gcaaggccat gccgtagatg aacggtgcct tgaggatttt cagctgggcg
     2281 gtggtttcgc cggtttccag gtactggccg ccggtttgcc acatcagcca gcccagggcc
     2341 agcaggacag ccccgcagat cagctgcatc agggcgcgct ggaccttgcg gacggtctcg
     2401 ggcaggtaat ggtccagcga gtcgaacacc acatgctcgc cgcgcagcga gaccagcggc
     2461 agggccgcaa aaatcaccac caccatcagc agctcggtga gctccaggga gccggtgatg
     2521 gagttggaga gcagcttgcg gcccccgacg tcaaaaaaag tcagcgccat gatggcgaac
     2581 agggcaaggg cggccagcag gccgcagacc ctctcgatga gcttgttcaa tctatgtgcc
     2641 tcaatccatg aaaaaaagcg cggccctcat tgtccatgag gccgcgccgg ggctggcgtt
     2701 cggacgtcag ccggttattt ttcctggcgg gctatttccg cgcggaactc ggccagcacc
     2761 ttggcggcgt ccttcaggcc cttggcttcg gcgtccttga tccacttctg ctccagcggg
     2821 gcggtcttgg ccttgacgtc ggcgacaaac ttcgcatcgg ccttgacgac ctgtacgttg
     2881 ttggcctgca tcaggcccat ggcgcgacga tccacgcggt cccagccgcg accgtaaata
     2941 cgcgcagcca cttcgccgga gatggcgtca acggctttct tctcgtcggc cgggagcttg
     3001 tcgtatttgg cctggttcat catgaagaca aagctggtgt tgtacaggcc accggggaag
     3061 gtggtggcgt gtttgatcag tttgtcgatc ttgaacgact cggtggactc ggccgggaac
     3121 agcgtgccgt ccatgacgcc gccggacagc agctcgtacg agtcgggtgc cggcttgagc
     3181 gtcacgttca tgcccagcgt tttcgcgatc tcgttgacca tgccgccgcc gacgcgccac
     3241 ttcaggccgc ccagctcggc cacgttggtc acgggccgtt tggtgttgaa aacaatcccg
     3301 gggccgtggg tgaagaagga caggaccttc acgccctggt gttccgcctg gaactgcggg
     3361 tttttcatgg ccaccttgtt gaaggccacg gaaatcggct cg
//
LOCUS       DAIDFG010000330         3374 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_330_length_3374_cov_16.176559, whole genome shotgun sequence.
ACCESSION   DAIDFG010000330 DAIDFG010000000
VERSION     DAIDFG010000330.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_330_length_3374_cov_16.176559"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          581 a         1049 c         1069 g          675 t
ORIGIN      
        1 accttgcgct cggtaccctg catgttcagt acgcccttgt agcgcaacat gcgcgggccg
       61 tagatattga cgacggcgcc caggaagtcc tcgagtttcg ccggatcaaa agcacgctcc
      121 gaacggaaca caaaactctt gacgtcgtca tcgtgatggt ggtgatggcc gtgtttgtcg
      181 tccttcgcat gggacgggtg gtcgcaatgc tcgccgtgtg cgtggtcatg gccggcatgg
      241 tcgtgctcgt cggccttgag gaagtccgga tcgatgtcga gtttggcatt gaggttgaag
      301 ccgcgcaggt caaacacgtc ggcaatcgcc acctcgccaa aatgcacggc tttctgcggc
      361 gcgcgcgggt tcatgtgttt gaggcggtgc atcagcgcct ggacttcgtc ctcggccacc
      421 agatcggcct tgctgatgaa gatctggtcg gcaaaaccga cctggcggcg cgcctcctgg
      481 cgctcgttga gctgggtggg cgcatgtttg gcatcgacca gggtcagaat ggagtcgagc
      541 aggtactgct cggcgatttc ctcgtccatg aaaaaggtct gcgccaccgg gccgggatcg
      601 gccaggccgg tggtctcgat caccacccgc tcgaaatcca gcaggccctt gcgcttcttg
      661 gcagccagca gctgcagggt ctcgcgcaga tcctcgcgga tcgagcagca gatgcagccg
      721 ttgttcatct ggatgatgtt ttcctgggtg tcgttgacca ggatctcgtt gtcgatgttt
      781 tcctcgccga actcgttttc gatcacggcg attttctggc cgtgggcttc ggtcaggatg
      841 cgcttgagca gcgtggtttt gccggagccc agaaagccgg tcaggatggt cacgggaatc
      901 aaactcatgg tgtctgccta aaaaaatacg gggaaaaata caggagaaag agaaacggtg
      961 ggcccgtgaa ggtggggagt ttagtcgtca acacaaggcc ctgaagcgca cattgactcc
     1021 acacggggcc tgccggacca ctttacaggc cgtgtcaagc attccagcgc ctgcgcctct
     1081 tttttgctat gttatcaata gctgactgcg cccattcaat aagggcagag gccatttttg
     1141 atgatgaatt ttattttttg agcaagacca gccccttgag gtactcgccc tcggggaaac
     1201 tgacggtcat cgggtggtcg ggcgcaccgc ccaggcgttc ggtgatgaag gcatcgacgc
     1261 ccgcatcggt gccggcggac gcaacgatct tgtgaaacag gtcggcgctg atgccgcctg
     1321 agcaggaata cgtgaaaagc acgccgccgg gcgccaggat ggacagcgca atgcggttga
     1381 tgtccttgta ggcacgtgct gcacgttcgg catgggccgc ggtgggcgca aacttgggcg
     1441 ggtccagcac gatggcgtcg aaggtccggc cttccttgtg gaactgccgc atcgaggcat
     1501 tgacatcggc gtccagcatg gtggtgcggg cggcgtcaaa accgttcagc gccacattgg
     1561 ccgcagcttt ttcaatcgcc ggtccggacg aatcgatcga cgtgacatgc gctgcgccgc
     1621 cggccagcgc cgccaccgtg aagcccccgg tgtagcagta acagttgagc acgttctgga
     1681 actgcaggcg gcgcgcatgt tcggcaaact tgcggcggct gtcgcgctgg tccaggtaaa
     1741 agccggtctt gtggccggtg gcgatgtcca ggcccagctg ccagccgtgt tcggtgatga
     1801 cgaggtcggt ggcgccactg ccggccagcc agcccgtcgc ctccggcaag ccttcgagcg
     1861 aacgtacgct ggcgtccgag cgctcgtaaa gccgcgtgag tccggtttcg gccagcaggg
     1921 cttcgacgat cacgcttttc cagcgctcga cgccgcagct cgaaaactgt gccaccagcg
     1981 tgtcggcata caggtccacc accaggccgg gcaagccatc ggactcgccg tgcaccaggc
     2041 gcattccgtc actttgtatg tcaaaacggc tcctggccct tatagcgcga gcgatacgcg
     2101 ctacaaaaaa cgaagcgtcg atacactcgg tctcgacaaa actccagacg cgggcgcgaa
     2161 tcttcgaact cgggctgaag gccgcccagc cgaggaactg cccgtcgtgg ccctcgaccc
     2221 gcaccgtttc accagcatcc gccccgccgc gggcaatcgc accgtcaaac acccagggat
     2281 ggcgccgcag cagcgaacgc tccttgcctt ctctcagccg tattgatttc atgcgtctat
     2341 tgtcgcggca gcggctcccc cgaaagtggc ctccgggaaa tttgcgacaa tcacttgatg
     2401 ttttttgagg tcttttcagc gcattttgca ggcagcaagg catggattcg tccgtgcgcc
     2461 ggcctgtggg cggccttcgg actggccctg gtgctggccc tcctgagcct gcccgcgctg
     2521 tcccgcacgg tgctggacct cgacgcagag aagcagccgg tcgcgctggc cgactggggc
     2581 gactactgga tagacccgca atcgcaactg gcggtcgaac aggtcgtcaa tcaacgtgac
     2641 ctgggctggc agcagacccc ggcgcgcggc atctatccct tgctgcccgg acagtcgctt
     2701 tggatccgtt tcatcgtgcc gccagcgccc gacatggagc gctggctgct tgaaattccc
     2761 taccctgcgc tggaccgggc atcgctgtac acgctggaca gcgccggcca gtggatagag
     2821 cagcgcgccg gcgacctgac gccggtcaac ctgtggccca cgccgcatcg ccaccccttg
     2881 ctggtggtca acttcaatgc cgaggacccg acgcattaca tcctgcggat tgacaacgcc
     2941 caggggttca gtgcgcccat ccgctttgtc agctccgggc atgtgctgcg cagcgaacag
     3001 caggtgtcgc tgttcctggg gatttatttc ggcctggcgc tgctgggctg caccgccggc
     3061 ctgatcggcg ctttctggct gcgggaccgg gcctacctct actatgcggc gtgttccgcg
     3121 ctgatcggcc tgacgcaggt ggccatcacc ggcgtgggcg gcctgcacct gtggcctgat
     3181 tcaccggcct ggacagatcg gtcgctgacc gtgctggcca cctggatgct ggtgtcgctg
     3241 ctgctgctca atgccaccgt ggtgtcgctg gcgcagcgct cgcgcatgct caacctcctg
     3301 gtgtgggccg ccgcgctggc tggcgccggc ctctcggtcg cgctggcgct gaccgacagc
     3361 gcgctgcgca tcac
//
LOCUS       DAIDFG010000331         3356 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_331_length_3356_cov_15.698879, whole genome shotgun sequence.
ACCESSION   DAIDFG010000331 DAIDFG010000000
VERSION     DAIDFG010000331.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_331_length_3356_cov_15.698879"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          599 a         1060 c         1068 g          629 t
ORIGIN      
        1 gtcggacgcg ccgatcacat cgaggttatg ttcgatgacg atcagcgaat ggccggcgtc
       61 gatcagcttg cggaacgagc gcatcagctt ggcgatgtcg tcgaagtgca gcccggtggt
      121 cggctcgtcg aacaggaaca gcgtgccgcg gttgatgttt ttcagcgcca gcggctggcg
      181 gctgttgctc gccgttttcg atgcgccggc caggaagccg gccagcttga gccgctgcgc
      241 ctcgccgccc gacagcgtcg gcaccggctg gcccagcttc acgtattcga ggccgacatc
      301 gacgatcggt tgcagcgcgc gaatcacctc ccggtcctga gcaaacagcg cacaggcctc
      361 gctcacggtc aggtccagca catcggacac gttcatcagt cgtgaacgca catcaccgat
      421 cgccttgcgt tcaatggtga cttccaggat ctcgggccgg taacgcttgc cgtcgcagtc
      481 ggggcaacgc aggtagacgt cgctgaggaa ctgcatctcc acatgttcga agcccgaacc
      541 gccacaggtc gggcagcgcc cgtcgccatt gttgaaactg aatttggacg cggtgtaact
      601 gcgctggcgg gccatgggcg cttgcgcaaa cagctcgcgc acggcgtccc aggcaccgac
      661 gtaactggcg ggattcgagc gcgccgtctt gccgatcggc gactggtcga caaacaccac
      721 gtcggtcagg tagtcggcgc ccatcagccg gtcatgcagg cctggtgttt ccgtggcctt
      781 gccgaactgg cgcaacagtg cgggcgccag gatgtcctgc atcagcgtgg acttgccgga
      841 gccgctgacg ccggtgacac acaccagacg ctgcagcgga aactccaccg tcagattttt
      901 caggttgtgg tcgcgcgcac cttccaggat caggcgcggc gtgttgtccg tcaccgggcg
      961 cttgaagccc atgcccacct gtttgcgcga gccgagatag gcgccggtca acgtatccgc
     1021 gtgtttgagg tcttccggtg tgccgtcgaa aacaatctgg ccgcccttct cgcccgggcc
     1081 aggacccata tctatcatgc ggtcggccgc cagcatcacg gccgggtcgt gctcgaccac
     1141 caccagcgtg ttgccggcat cgcgcaggcg gtgcatggcc tgggtgatgc ggttcatgtc
     1201 gcgcgggtgc aggccgatgg acggttcatc gagcacgaac agggtgttga caagtgaagt
     1261 gcccagcgcc gtcgtcaggt tgatgcgctg cacctcgccg cccgacagcg tgcggctttg
     1321 ccggtccagc gtcaggtaac cgatgccgac atcactgagg tatttgagcc gggtgtggat
     1381 ttcctcgaac agcgacttga gcgccttgaa ctcggcatcg ggcgcgccgc cgtcctgcag
     1441 cgcggccggc gccagcccgt tgaaaaattc acgcagccgg tcgatcggca gctgcatcag
     1501 gtcatgcagg ctcaagccgg gcaatgcctc gagctgcgcg cgggtccagt ccacacccac
     1561 aggcatgaaa cgtttgccag gcggcagcgc cgcatcggcg gcttctttcg agcccagacg
     1621 ccagagcagg gcttcgagct tgaggcgcgc gccgccgcag gtctcgcaag gcgtgtagct
     1681 gcggtacttg gacagcagca cacgaatgtg catcttgtag gccttgcttt ccaggtaggc
     1741 gaaaaagcgc ttgatgccat accactgctt tttccactga ccttccttgt aggccggcgt
     1801 gccgtggatc acccagtgct gctgcgccgg cgtgagtttg taccagggcg tatcgcgcgg
     1861 gatgcctgcg gcttccgcat ggcgcatcag gtcgtcctgg ttttcggccc aggccggcgt
     1921 ttgcagggtc ttgatcgcgc cggagcgcaa cgtcagcttg tcgttgggaa tcaccagttc
     1981 gtaatccaca ccgatgacgc ggccgaaacc gcggcaggtg tcacaggcgc ccatggccga
     2041 gttgaaggaa aacagcgagg gggtcggatc ggcgtagcgc aggtcgcttt cggggcaatg
     2101 caggccggtc gaaaagcgcc acatctcctc cacggcgccc tcctgcgccg gctgcacata
     2161 gacattgagg cgaccaccgc gtttgagcgc cagctcgatg gcttcgatca cccgcgcctt
     2221 ctcggccgtc tgcacacgga agcggtcggc caccacatcg agcaatttat gcggcccggc
     2281 cggagtcgcc acgtcgcgct ccgcatgcac acgcgtgaag ccgctggccg acagccactg
     2341 cgtgacctcg tcggcgctgg tgttgcccgg cagttccacc gggaaggtca tgaccactct
     2401 tgaatcgctg cccgcggtgc gcgccatcaa ctcggcataa atcgactccg gcgtgtcatg
     2461 gcgtacggcc tgcgcggtct tcttgtcgaa tagctgcgcg gcgcgtgcat acaaaagttt
     2521 taaatgatcg ttgagctccg tcatggtgcc cacggtggag cgcgagctgc ggaccgggtt
     2581 ggtctggtcg atcgcaatcg ccggtggcac cccttccacc ttgtctaccg cgggtttgtc
     2641 catgcggtcc aggaactgcc gcgcataggc cgaaaaggtt tccacataac ggcgctggcc
     2701 ttcggcaaaa agcgtgtcaa acaccagcga ggacttgccg gacccgctgg gcccggtgac
     2761 caccgtcatc tccccggtgc ggatatcaag atccaggttt ttgaggttgt gctgtcgcgc
     2821 gccgcgaatg cggatggttc cctgggtcat gggtgggggg ctttccttgt aggtcaaaca
     2881 ttctagacag cgcccgggtt gacagtcggc gtagggttaa accgcccgcc gggcctgtgc
     2941 gctggcctgt attctggtta tcccgcgcgt agtttccaca ccgaacggtc acggtggcag
     3001 cgtaaactcg atcgccgaag gagataagac atgaaaaagc tgaatttctg gtgcgctgca
     3061 cttttgctcg cgaccatgag ccacgccagc caggcacaaa tcctgatcgg gcagaccgcg
     3121 ggctttaccg gccctgtggg tgccggcgta aaggaaacca cggcaggtgc gaagctctac
     3181 atcgacgccg tcaatgccaa aggcggcgtc aacgggcaga agatcgagct ggtctcgctc
     3241 gacgacaagt tcgaccccaa gctggccggt gaaaatgccc gcaagctgat cgaggaacag
     3301 aacgtggccg ccatgttcct gacccgcggc acgccccaca cggaagccat tcttcc
//
LOCUS       DAIDFG010000332         3353 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_332_length_3353_cov_15.738629, whole genome shotgun sequence.
ACCESSION   DAIDFG010000332 DAIDFG010000000
VERSION     DAIDFG010000332.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_332_length_3353_cov_15.738629"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          622 a         1029 c         1075 g          627 t
ORIGIN      
        1 cacggcaaag attggcagcg caaacagcat gcggtacatg atgagcgtga ccgcatcgac
       61 gccgtagcgg taggcgagct tgacgatgat cgccttgccg ctgaaggcaa tcgcgccaaa
      121 cgtcgccagc agcaacccgg tcgctatgct tttaggagct gtctgcgccc gtggatcctg
      181 ggctgcaagt gttttttgct ctgaacttct ggacaagtgg atttatcaga aaccgacggc
      241 ctggccgtcg cgccggctgt cgctggccgc cacatacccc tcgaccttgg gatcgccggc
      301 acggcagatg aactggcccg cgccgaagtc ctggtaggag tcgttgatca cttccatgcg
      361 gtggccgcgg tccgacaggc cctgcacggt ctgctggttc attgccgctt cgacattgat
      421 ttcaaaaccg gcgttgtagc gccagcgcgg cgcatcacag gcggcctgcg ggctctgccc
      481 gtagtcgagc atgcgcacca gcgtctgcat gtggccctgg ggctgcatgt tggcgcccat
      541 cacgccataa ctcatcaccg gttgaccgtc tttcatgagg aaggccggga tgatggtgtg
      601 gaaaggccgc ttgcccggcg ccacctggtt cacgccggcc tgcacgctga acgcgtggcc
      661 gcggttttgc aaactgatgc cgaagtcggg ctcgacacaa cccgagccga accccatgta
      721 gttgctctgg atgaagctga ccatcatgcc gtcttcgtcg gccgcggtga ggtaaatggt
      781 gccgcccttg accgggttgc cggcgccgaa atcctgcgct tttttcatgt cgatgagttt
      841 ggcacgggag gcgaggtagc tgtcatcgag catttgatcg acgctcacct ccatagccga
      901 tggctcggcc acatatttgt aaacatcggc aaaggccagc ttcatggcct cgatctgcag
      961 gtgctgcgaa tccaccccgt ccactgccag gccggccaca tcgaacttgt ccaggatgcc
     1021 cagcgcaatc aaggccgaga tgccctggcc gttcggcgga atctcgtgca gcgtgtagcc
     1081 gcggtagtcc ttgccgatgg gcttgaccca ctccggctgg taggacgcaa agtctttggc
     1141 cgtcaggctg ccgccgtttt gggcagagaa tttttcgatc gcctgcgcga tctcaccgcc
     1201 gtaatacgcc gcgcccttgc tctgggcgat cgcccgcagc gccctggccg cggccttgaa
     1261 ctggaacagc tcgcccacat tcggtgcgcg gccccagggc agaaaggact gggcaaaacc
     1321 gggcatcgct ttcagttcat tggtagcggc cgcccacttc tgctgaacca ccggcggcag
     1381 caggtagccc cgctcggcaa tttcgatcgc gggctccatc aggtcggcaa acggcagccg
     1441 gccaaaacgc tcgctcatgg ccacccagcc ggccacggca ccgggcacgg tcaccgaatc
     1501 caggccgcgt ttgggcgggg tggcggcgcc gtcgccgtat ttgcgcttga agtagtccgg
     1561 cgtccaggcc tgcggtgcgc ggcccgaggc attgaggcca tgcagttcct tgccgtccca
     1621 caggatgcag aaggcgtcac tgcccaggcc gttgctgacc ggctcgacga tggtcatggc
     1681 agcggccgcg gcaatggcgg catccaccgc gttgccaccc ttgagcagca tgcgcagccc
     1741 ggcctgcgcg cccagcgggt gcgaggtgga caccacattg cgggcaaaca gggggatgcg
     1801 ggtggaggtg tagggattgc tgtagtcgaa gttcatgaat ggctctgaaa aggaaagggc
     1861 tggcgccagg ccatggcgcc agttaaacgc tgtcagtcgg tggtgatctt gcgttcggaa
     1921 atgatttttt tccacttggc gttgtcggtc ctgaccatcg ccgcaaaagc ctgcggcgtg
     1981 ccgccggtgg gctcggcgcc cagccgcgca aaccgctctt tgacatcggg gtcggccagt
     2041 gccgcattga gcgcctggtt cacccgggcc gtcagctcgg cagtcaggcc tttggggccg
     2101 tacacgccaa accaggtcac cgactcgaag cccggcagga tctcggacac cgtcggcaga
     2161 tcaggcgcga gcgcggtgcg cttgagagtg gtcaccgcca gggcgcgcag cttgccatcc
     2221 ttgacatgcg gcatgccggt cacaaaagag tcgaacagta cgtcgagctt gccgctgatc
     2281 aggtccggca tggccagcgc cgtgccccgg tagggaatat gaagaatgaa ggtgccggac
     2341 tgcgccttga agtactcggt ggtcaggtgc acgatggtgc cgttgccgct ggatgcgtag
     2401 ttcagccgcc ccggattttt tcgcgcatgg gcgatgaact cctgcatggt cctggccggc
     2461 gaggagttgg gcaccagcac gacgttgggc gaactggcga cgtaggcgat cggtgtgaaa
     2521 tccgtctcgg cgttgtaggg gattttcggg ttgatggccg gcccgatgct gtgggtgctc
     2581 gaggtcgcca gcagcagcgt gtagccatcc ggcgtggcct tggacgcggc gtcggcgcct
     2641 atggtcccgc cggcacccgg ccggttgtcg accaccacgg tctgcccgat tttttcaccg
     2701 agcttctgcg acagcgcacg cgccaggatg tcagtggctc cgcccgccgg gaaaggcacg
     2761 atcatgcgga tcggccgtgc gggataagct gctgcagaag tctgcgccgc ggcgggcaaa
     2821 gcggcagcaa ccgcaacgct ggccgccgtc ataaaaatga tatgtctgcg tttcatttcg
     2881 ccattgtgca acggttttca ggctggctgg gtcagtacag ttttacaaaa aaaacggcac
     2941 ccgagggtgc cgttgggtca tgtcatcgag ggatcagtcc tcgacaaaag cctcttcacg
     3001 cttgttcttg accgcaggca gaagcacgat cagcagcagc aacgctccgg cggcaagcag
     3061 gccggcagac agcggacggg tcacgaacac gctccagtca ccgcgcgaga gcaacagcgc
     3121 gcgacgcagg ttctcctcca tcatggggcc caggatgaaa cccagcagca gcggcgcagg
     3181 ttcagctccg agcttgatga aaatataacc aatcacaccg aaaatcgcca ccatccagat
     3241 gtcccaggtg ttgttgttgg tcgaataaac accaatcgca cagaacaaca caatcgccgg
     3301 gaacagcagg cggtagggga tggtcagcag cttgatccag atgccgatca gcg
//
LOCUS       DAIDFG010000333         3330 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_333_length_3330_cov_19.178626, whole genome shotgun sequence.
ACCESSION   DAIDFG010000333 DAIDFG010000000
VERSION     DAIDFG010000333.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_333_length_3330_cov_19.178626"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          702 a         1032 c         1023 g          573 t
ORIGIN      
        1 atttacgagg cggtgtccgg tgcgcgcatg cacgcggcat atttccgtcc cggcggcgtt
       61 taccgcgacc tgccggacac catgccgcag tacaaggtca gcaagatccg gaatgccaag
      121 gccatagagg cgatgaacga gaaccgccag ggttcactgc tcgatttcat cggggacttc
      181 acccgccgtt tcccggctta tgtggacgag tatgaaacgt tgctgacgga caaccgcatc
      241 tggaagcagc gcacggtggg tgtcggtgtg gtttcgccgg aacgcgcact cgccatgggc
      301 atgaccggtc cgatgttgcg cggctccggc atggcgtggg acctgcgcaa gcagcagccc
      361 tacgaggttt acgaccgcat ggatttcgac attcccgtgg gcaagaccgg cgactgctac
      421 gaccgctatc tggtgcgggt cgaggagatg cgccagtcca accgcatcat cgagcagtgc
      481 gtggcctggc tgcgcgccaa tccggggccc gtgatcacca gcaaccacaa ggtggctgct
      541 cccgggcgcg agtccatgaa gtccaacatg gaggagctga tccatcattt caagctcttc
      601 acggaaggcc tccatgtacc cgaaggtgaa gcctatgccg cggtcgaaca cccgaagggc
      661 gagttcggca tttatatcgt gagcgacggc gccaacaagc cttatcgcct gaagatccgc
      721 ccgccgggtt ttgcgcacct tgctgcgctg gacgaaatgg cgcgtggcca catgctggcc
      781 gatgccgtgg cgatcatcgg aaccatggac attgtttttg gagagattga tcgatgatct
      841 ctgaagttac caaagcgcgc ttcgcgaaag aagtcgcaaa atatcccgcg gaccagaagc
      901 agtcggccgt catggcctgc ctggcgatcg tccaggaaga gcagggctac gtcagcgccg
      961 agagcgaaaa actcgtggca gagtacctcg gcatggcacc gattgccgtg catgaggtca
     1021 cgaccttcta caacatgtac aaccagcagc cggtgggcaa gtacaagatc aatgtctgca
     1081 ccaacctgcc gtgccagctg cgcgatggca ccagggcgct ggcctacctc gagcacaagc
     1141 tgggcgtgac catgggcggc acgacggaag acggcctgtt caccctgcag cagtccgagt
     1201 gcctgggcgc ctgcgccgac tcgccggtca tgctggtcaa cgacctgcac atgtgcagct
     1261 tcatgagcaa cgacaaactc gaccagctga tcgatggcct gaaatcgggc gaggctaaat
     1321 aaccatgtct gcttccagca tcctctccca gtttgaagcc accggcgtcc agacctcctt
     1381 ccacggccgc catatcgacc cgcagatcat ggcgggcctc gatggcgcca actggcgcct
     1441 gaaagactac gaagcgcgcg gcggttacca ggccctgcgc aagatcctgg gcaaggacgg
     1501 cggcgaaggc ctgacacaag accaggtgat cgccgaggtc aagggcagcg gcttgcgcgg
     1561 acgtggcggc gcgggtttcc cgaccggcct gaagtggagt ttcatgccgc gccagtttcc
     1621 gggccagaaa tacctggtct gcaactcgga tgaaggcgag ccgggcacgg ccaaagaccg
     1681 cgacatcatg cagtacaacc cgcacatcgt catcgaaggc atggcgattg ccgcctacgc
     1741 gatgggcatc agcgtcggtt acaactacat ccacggcgaa attttcacca cttacaagcg
     1801 gtttgaagag gcgctggaag aagcgcgcgc cgccggcttg ctgggcgaca acatcctggg
     1861 cagcagctac agcttccagc tgcatgcggc ccacggtttc ggcgcctaca tctgcggcga
     1921 ggaaaccgct ttgctcgaat cgctggaagg caaaaaaggc cagccgcgtt tcaagccgcc
     1981 gtttcccgcg agttttggcc tgtacggcaa gccgacaacg atcaacaaca cggaaacctt
     2041 cgcggccgtg ccgtggatca tccgcaatgg cggccaggcc tacctggact gcggcaagcc
     2101 caacaacggc ggcaccaaga tctttaccat cagcggcgac gtcgagcgtc cgggcaacta
     2161 tgaaatcccg atgggcacgc cttttgccaa actgctcgag ctcgcgggtg gcatgcgcgg
     2221 cgggcgcaag ctcaaggccg tgattcccgg cggttcgtcc atgccggtgc tgccggccga
     2281 catcatgatg gccaccgaca tggactacga ctcgatcgcc aaggccggct ccatgctggg
     2341 ctccggcgcg gtgatcgtga tggacgagac ccgttgcatg gtcaagagtc tgctgcgcct
     2401 gtcctacttc tacatgcacg agagctgcgg ccagtgcaca ccctgccgtg agggcacggg
     2461 ctggctctgg cgcatggtgg accgcattga acacggccat ggccgtgaga gcgacctgga
     2521 cttgctcaac tcgatctccg acaacatcca gggccgcacc atctgcgcgc ttggcgacgc
     2581 agcggccatg ccggtgcgcg ccatgatcaa gcacttccgc gccgaattcg agcaccacat
     2641 cacccacaag acctgtctgg tcccggttta cgcataacag cgctccaata aagcagattc
     2701 acatggttga aatccaactc gacggtcaga aagtggaagt gcccgaaggc agcacggtga
     2761 tgcatgccgc cgaaaaagcc ggcacctaca ttccgcactt ctgctatcac aagaagctca
     2821 gcatcgcggc caactgccgc atgtgcctgg tggacgtgga gaaggcgccc aagccgatgc
     2881 cggcctgcgc gacgcccgtg acgcagggca tgatcgtgca caccaagagt gacaaagcca
     2941 tcaaggccca gaagggcgtg atggagttcc tgctgatcaa ccacccgctg gactgcccga
     3001 tctgcgacca gggcggcgag tgccagctgc aggacctggc ggttggctac ggcggcagcg
     3061 gttcgcgcta cgaagaagaa aagcgcgtgg tgttccacaa gaacgtgggc ccgctgatct
     3121 ccatggagga gatgagccgc tgcattcact gtacgcgctg cgtgcgtttc ggccaggaag
     3181 ttgccggtgt gatggagctc ggcatgtcca accgcggcga acatgccgag atcgagactt
     3241 ttgtcggcat gtcggtggac tccgagctgt caggcaacat gatcgacatc tgcccggtcg
     3301 gcgcgctgac cagcaagcct ttccgctaca
//
LOCUS       DAIDFG010000334         3330 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_334_length_3330_cov_15.031450, whole genome shotgun sequence.
ACCESSION   DAIDFG010000334 DAIDFG010000000
VERSION     DAIDFG010000334.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_334_length_3330_cov_15.031450"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          617 a         1002 c         1116 g          595 t
ORIGIN      
        1 tgaccttcat gggggatgcg ttgcgggatg cgctggaccc ccggaaagcg gacaaatgac
       61 ggcccccacg ctccgcactg acgtgtcgtc gctgcccccc gagggggctg aacttgctcg
      121 gggcggccct tcgctgcgtt cgacggcctc cacgacctcc acgcccggca cggacgtgtc
      181 ctgccttctg gaggtcaaca atctgcgcgt gtccttcgcc ggcaaggaag tggtgcgcgg
      241 catcgatttt tccatccagc ccggggaaaa actcgcgctg gtcggcgagt cgggctccgg
      301 aaaaaccgtc accgcactga gtctgctgcg cctcgtgcag aacgctgcca tcagtggcaa
      361 ggcgaccttc gccggacatg acggggctgt cgacctgctg tccctgcccg agcgggcctt
      421 gcgcggtatc cgcggccagg acatcgcgat gattttccag gagccgatga cggccctgaa
      481 cccgctgttc accgtgggtg agcagatcgc cgaagtcttg cagctgaaaa aggctctgac
      541 caccgccgaa tctgcgcagg cagctatcaa attgctagcg gataccggca tccccgaggc
      601 cgcccggcgt gccggctcct acccgcacca gttgtcgggg ggccaacgcc agcgcgccat
      661 gattgccatg gcgctggcct gccagcccaa actgctgctg gccgacgaac ccacgacggc
      721 gctggatgtc acgctgcgca cgcagatcct ggacctgctg accgggctgc agcagcgcaa
      781 cggcatggcg gtgctcctga tcacccacga cctgaacctg gtgcggcgct ttgccgaccg
      841 cattgccgtg atggaggacg gacgcattgt ggagaccggg ccggtggcgc aggtgttcga
      901 ccagccgcag catgcctaca cccgcaaact gatagacagc cggccggcac gcgacgtggt
      961 ggagggccgg gccgatcctg ctgctgccgc cgtcctgcag gcccgggatc tgcgggtgac
     1021 gtatccgctg ccgattcccg gggtgcgcgg ctggtttcgc aagggcgagt ttgtggccct
     1081 ccatggcgcc agtttccagg ttcccgccgg ccagaccctg ggggtgatcg gggagtccgg
     1141 gtcgggcaaa tccacgctgg ccctggccgc gctgggtctg catccgcacc aggggcagct
     1201 gctggcggcg ggccaggcct ggagcggcga tgctgccagc aacaaacgta tccgccgagc
     1261 ggtgcaggtg gtgtttcagg acccgttttc ctcgctgtcg ccgcgcatga cggtcgagga
     1321 aatcgtcggt gaaggcctgc gcgttcacga gccggggctg tctgccggcc agcggcgtga
     1381 gcgggtgctg caggcgctgg cggacgtggg catgggcgag gcgcagagtt tcgtcgccgg
     1441 gccgccccaa gacgaaaagc acccccttgg tgggcagcaa gctacacgca gtgagggcgc
     1501 gcgggggcag tatttccccg gcctgctgca gcgttacccg catgagtttt cgggcggaca
     1561 acgccagcgg ctggcgattg cccgggccct gatcatccag ccccaattgc tggtactgga
     1621 cgaaccgacc agcgccctgg acgtgaccat ccagaaacag gtgctccagc tgctgcaaaa
     1681 actccagcgc gagcgcggcc tgagctatct gctgatcacc cacgacgtgg acgtgatccg
     1741 ggccatggcg catcaggtca tggtgctcaa ggacggcgaa gtgctggaag ccggcagcgt
     1801 ggaggaggtg ctggagcggc cgcggcatcc ttacacgcag accctggtgg cggcacaagg
     1861 ctgacgcgga ttttgtcaat tgagtctttt tgctgatttc gctgcttttc cggaatttgc
     1921 ggatgcttgc ccgatggggc ctgcgcaagg gcggcggcgg gtagggcgcc ccggcatgta
     1981 aactgcttgc acaggcgtca gcagcaggtt acaatatgcg cttcacgacc aaatgggcgg
     2041 gttttcaccg cccatttttt ttgggcgttt gtttttgcaa ccctgacttt tacgaagctg
     2101 cttttttaga actgaatggc attacacgag acgattgagc aaacagtcac gggactgggt
     2161 tacgagctgg tggacatcga gcgtaccgcc ggtggtttgc tgcgcgtgac gattgaccag
     2221 ccgtatgttc agggtgccga gcagttcatc aatgcggaag actgcgaaaa agtcacgcgc
     2281 cagctgcagt ttgtgctgga agtggaaggt acagcgtatt cccggctcga ggtgagctcg
     2341 ccgggcatag accggccctt gcgcagcgaa aaggatttcg agcgctttgc cggcgagttg
     2401 atcgacctca cgctcaaggc gcccatcggt gcggtggctg cggccgggtc cggggtgtcg
     2461 gccagccgga agaaatttcg cggcacgctc gagcgtgcgg catccgggtg gcagattgtg
     2521 tggagcgaag agcccgccgt caaacccggg caaaaggtaa gcaagaagag gattcccgca
     2581 ccgctgcagg cgctgggttt cacgctggat gaaatagtgc aggcgcgtct ggcgcccgtt
     2641 gttgacttca aggggcgcag gccgaaaagc gcacccgcca cagagatata aaagaaaggc
     2701 aggcttgtga aatgaatcgc gaactgttga tgttggttga tgccatatcg cgtgagaaaa
     2761 atgttgaacg tgacgtcgtc tttggcgcgg tcgagcttgc gctcgcctcg gccacgaaaa
     2821 aggtgtacgc agacggtgtg gacattcgcg tggtggtgga ccgcgatagc ggcaactatg
     2881 aaaccttccg ccgctggctg gtggtgcctg acgaagccgg cctgcaaaac ccggaagccg
     2941 aagagctggt cagcgatgcg cgcgacgaga ttgcggacat cgaagaaggc gactacatcg
     3001 aaaagccggt tgaaagcctg ccgatcggcc gcattggcgc gcaagcggcc aaacaggtca
     3061 tcctgcagaa aatccgcgac gccgagcgcg agatgctgct caacgacttc atgtcgcgtg
     3121 gcgagaagat ttttgtcggc accgtcaagc gcatggacaa gggcgacatc attgtcgaat
     3181 ccggccgcgt tgaaggccgc ctgcgccgca gcgacatgat tccgaaggaa aacctgcgct
     3241 ccggtgaccg cgttcgcgcc atgatcatgg aagtcgacaa caccctgcgc ggcgcgccca
     3301 tcatcctgtc gcgtaccgcg cccgagttca
//
LOCUS       DAIDFG010000335         3329 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_335_length_3329_cov_17.956323, whole genome shotgun sequence.
ACCESSION   DAIDFG010000335 DAIDFG010000000
VERSION     DAIDFG010000335.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_335_length_3329_cov_17.956323"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          566 a         1082 c         1079 g          602 t
ORIGIN      
        1 cagcgccagc tgttcggcca gcacgcgcgc gtcgatcagg gcctgggccg atccgttgga
       61 gccgcgcggg tacatggggt gggccgcgtc gcccagcagg gtcacgcggc cgaaggtcca
      121 ctgcggcacc gggtccttgt cgaccatggg gtactcgagg atctgctcgg cgttggcaat
      181 cagcgccgga acgtccagcc acgggaagcg ccagtccttg aagatgccct ggaaatccct
      241 gggatcgcct ttctggttcc agtcgttcat ggcggcgttg tcccgctgga tctcggcgac
      301 ccagttgatc agctggtggc cctcgtcgtc cagcttgtcg acgatgggat aaatcaccat
      361 cttgccggtg tcgatggagc cgatgcgcat gtagctgcgg ccggtcagga tgggtttgtg
      421 ccgcgtcacg ccgcgccagg tgttgatgcc ggcaaacgcg actttttcgt cggggtagaa
      481 ctggcgccgc agcgtggagt tgacgccgtc gcaggcaatc accacgtcgg ctttcaccgg
      541 cggcaggctc atgccgctgg aggtctgcat gaaatgcagg gtggcgccgc tctcgttctg
      601 ctccacacgc acgcagcggc ggttggtatg gaccgcgtcg gggctcaggc gctcgagcgc
      661 cgcttcgtaa agcaggcggt gcagcttgcc gcgcggaata ccgatctcgg gcaaggcgta
      721 gccggcatgg cggccgcgcg gctccttgta gatgaactgg ccgaagcggt tgaaaaacac
      781 gctttcctcg ttttcgatgc cgacggcttc gagctgctcc tgcaggccca gcgccgccag
      841 ctcgcgcatg gcgtgcggca gcaaggtgat gccgacgccg agttccttga cttcaggcac
      901 ggtttcaaaa acctcgctct ggatgccgcg ctgatgcagc gcaagcgcca ggctcagccc
      961 gcctatgccg ccgccgacga tggcaatttt catggtgttc tcttcgatag tttcaactgg
     1021 actgtcattg cggacttgat ccgcaatcca tgcaggcacg ccgcagcccc ggttcatgca
     1081 gcatggatcc cggatcatgt ccgggatgac ggcggatata aataatgaaa atcaaattgg
     1141 ccgccagccc ttgatagacg ggcgccacca gctatctatt cagtagcaaa tcagttcgcg
     1201 gtgatgccct gggcctggat caaaccggcc cagcgcttcg catccagctc gaccaggctc
     1261 ttgaactcgc ccggcgtgct gccgctcggg tccatgccct gggtctggaa ggccttcttg
     1321 acctcgtcgc tcagcaaaat ctcccggatc tcgcggttgt acagggcgac caggtcgggc
     1381 gaggtgcccg gcggcgcaaa aatgccgtac cacatgtcca catggacctt gccggctctg
     1441 gcctcggcca gcgtcggcac ctcgggcaac agcgcgtggc gcttgtcgct gccgatgccc
     1501 agcgccacca gccggccgga cttcacatgc ggcagggcca cgtggatggg caggaacatg
     1561 gtttcgatct ggccgccgag caggtcggtc agcgccggac ccgtgccgcg gtaagggatg
     1621 tgcgtgacaa acacacccgc cgtgcttttg aacagctcca tcgccaggtg gtgcggtgtg
     1681 cccaccccgg gcgagccgta gttgatgcgg ccgggcttct gccgcgccgc cgccatcagg
     1741 tccgaggcgc ttttgaaacc ggtcttgggg ttggccacca gcagcaactg gccccagctg
     1801 gtcagcgtca ccggggtcag gtccttcacc gggtcgaagg tcagctgcgg gtacaggctg
     1861 gcattcatca ccaacgtgtt gaccgtcacc agcaaggtgc tgccattggg cgcggcacgc
     1921 accacggcct cggtgccgat gttgccggac gcgccggcac ggttgtccac caccaccggt
     1981 cgccccaggc gctcggacag ccgggggccc agcgtgcggg caatcaggtc aatgccggtg
     2041 cccggcgtga aaggcacgat caggcgaatc ggcgcctgcg gcagggccgg cacctgggcg
     2101 gtctgcgcgc tctgggccca agcgcccgcg ctggcgccag ctgccaccag cagaagcccc
     2161 agcttgaccg ccagccgacg gcgtggagaa atactgatct gcatcaaaca tccttttagg
     2221 tttgcatgct aacaattatt aacccgaagc gacaaggcac aatccatgaa ttcccttaga
     2281 gcttgagtca acgagaactt tttactgccg tgcccgcctt ccacaaactg acccaacttt
     2341 atgcccgccc cggctttctg ctgcgccggg cgcatcagat ctcgaccgcg gttttcgagg
     2401 acgaatgccg cagcgtgggg ctgacgccgg cacagttcgg cgtgctcagc gtgctgcgcg
     2461 ccacgccggg gctggaccag tccacgctgg cacgtgcgct gggtttcgac aaggtgacgg
     2521 tgctgcgggt gctgcgcggc ctggagacgc ggggcctgat agaacgcgtg ccggcgccgg
     2581 ccagccggcg caatctggcg gtgtcgctgt cagccgcggg gctggacctg ctcaagcagg
     2641 cgcagcaacc ggccgatcgg gcctatgacc gcctgatggc accgctgaac aaggcgcagc
     2701 aaggccagct gctggagttg ctgcaactgc tgacgacggg gctggaaaac gacgcccgcg
     2761 ccgcctttgt gccgcaggac aaggaagccg acgcgcaagc ctgagccgag ggtctaggcg
     2821 ttggaggcct ggatgcccca cttggccagc gccgcgtcgt cgctgacccg tgcatcgacc
     2881 cagcgcgcgc cctccggcgt ctgctctttt ttccagaacg gtgcctgggt cttgaggtag
     2941 tccatcagga attcgcaggc ctggaaactc tcgccgcgat gcgccgaggt caccgccacc
     3001 atcaccacct ggtccagcgg ctgcagcagc ccgacacggt ggatcacgcg cgcaccgaaa
     3061 atatcgaagc gcagcatggc ttcgtcaatc atggcttcga tggctttttc agtcatgccg
     3121 gggtagtgct cgagctccat gctgctgacg ttcaggccat cgttgcggtc tctgaccgtg
     3181 ccgatgaaac tgcagaccgc accgacacgt ttgtcgttcg cgcgcagcgc ggcgatctcc
     3241 tcggcgagat tgaagtcgcc ggtctggatg ctcacgcgat cggccatctc agccccccgt
     3301 caccggcgga aagaaggcga cttcgcagc
//
LOCUS       DAIDFG010000336         3322 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_336_length_3322_cov_18.482400, whole genome shotgun sequence.
ACCESSION   DAIDFG010000336 DAIDFG010000000
VERSION     DAIDFG010000336.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_336_length_3322_cov_18.482400"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          507 a         1012 c         1140 g          663 t
ORIGIN      
        1 caggcacgac cagcgcgagc ttgacgttgc cggcgacctt cttgccgatt ttctggagga
       61 tggcggccgc ttcgcgcatg tcctcgatgc ggctgttggt gcacgagccg atgaagacct
      121 tgtcgaccag gatgtcgttg atagccttgc cgggctgcag gcccatgtag gtcagggcgc
      181 gttcgatggc gtcgcgcttg ttcgcgtctt tttccttgtc cgggtcgggc acgcggtcgt
      241 cgatggacag caccatctcg ggcgaggtgc cccaggtgac ctgcggccgg atctggctcg
      301 cgtcgagctg caccacggtg tcgaacacgg catcggcatc ggactgcagc gtgctccagt
      361 actgcgccgc ctgctcccac tcgacgccgg tgggtgccaa cggctttccc ttgacgtagg
      421 cgatggtctt ggcatccacc gccaccaagc cggctctggc accggcttcg atggccatgt
      481 tgcagaccgt catgcggcct tccatgctca gcgcgcgtat cgccgcgccg ccgaattcaa
      541 tcgtgtagcc ggtgcctccc gcggtgccga tttttccaat gatcgccagc acgatgtcct
      601 tgccggtcag gcccggggcc agcatgccgt ccacgcggac cagcatgttt tttgcttttt
      661 tggcgagcag ggtctgcgtg gccatcacgt gttcgacttc gctcgtgcca atgccgtgcg
      721 ccagtgcgcc aaacgcgccg tgggtcgagg tgtgcgagtc gccgcagacc acggtcatgc
      781 cgggcagggt ggcgccggtc tcgggcccca tcacatgcac gatgccctgg cgtttggaca
      841 ggaaggggaa gtaggcggcc gcaccgaact cggcgatgtt gctgtccagc gtggtgatct
      901 gttccttgct gaccaggtcg gtgatgccgt catagccgag ctcccagccg gtggtcggcg
      961 tgttgtggtc ggcggtggcg acgatggagc tgatgcgcca gaccttgcgg ccggcttcgc
     1021 gcaggccctc gaaggcctgc gggctggtca cctcgtgcac caggtgccgg tcgatgtaga
     1081 ggatggctgt gccgtcttcc tcggtgtgga cgacgtgttc gtcccagatc ttgtcgtaaa
     1141 gggtgcgtcc catgtgtttt tccttcgtgg tctgtcgatt ttaagggggg aggggtgcgc
     1201 cgtccggggt caccgcacgc gcagtgaggt ggtcgaccaa caggcgagcg gtgacgggca
     1261 ggctggaaaa atcgcgtgcc accagggcta tctggcgcga cgcccaggca tcggtcagcg
     1321 cgaccgattc gagttcgccg acgccgtgca tcagttcgaa cgcccgctgc ggcatcacgc
     1381 cgacgcccag gccgttgtgg atcatgcggc acattgcgtc cagcccggtc acgtggatgc
     1441 gcagcttgat cgtgcggccg gcctgcgccg cggcatcgcg catggccagg tagatcgagc
     1501 tgttcgaatg caggccgaca tggtcgaagt cgaggctgtc cgcaaaggcg atgctgtccc
     1561 gcgtgcccag cacatggccg cgcggcacga tcatcacgag gcggtcctgg cggtagggca
     1621 gggtctgcaa cttgccgatg ccgtcggccg tgttgcagat gccgagatcg gccgcgcctt
     1681 cctgcaccgc gcgcacgact tccgtgctca ggtgctcctc gaggtcgatc ttgacctccg
     1741 gatgctggct gatgaaggtg cccaggtctt ccggcaggaa ctgtacgatg gccgagatgc
     1801 tggcatgcac acgcacatgg ccgcgcacgc cggccgcgta ttcgctgagt tcgccctgca
     1861 ttttctcgag gctgaacagc accgaacggg catggtgcag caggctctgg ccggccggcg
     1921 tcaggtccac gccgcgcgtg tggcggtaca gcagcggggt gtcgagcgcg gcttcgaggt
     1981 cggacaggcg tttgctgacg gccgacgcgg caatgaattc ccgctccgcc gccttgccga
     2041 tgctgccaag ctcacacacg gccacaaaca attgcagcga cgtgaggtcc atgcggcggg
     2101 cgaaattgcg ttctgagctg ctcatcaaag gggtcaaagt catcgtggtt cacgatgttt
     2161 gcctattttg accgctttgt tcgggctctg tcatcgcgga atgcgatgac tggcttgccc
     2221 gctggctggg gcaggcgcgg ccgtccgcgg ggaggctttc aggtgttttt ggcctccagc
     2281 ccagcgggga cgggcgcaag cagctattta attgatagca atcagtcgat gatcccgccg
     2341 ggatcgagcg cccgcacccg gtggtccacg ttgtagccgc cgaactcggt gtaacgcagc
     2401 aggtgccgct cgcagcggcc gcagtgccag acctcgcagc ggttgtaggg gaaaaagccg
     2461 acggccaccg gggcatcggg cgagtcgtaa cgggtgcctc gggggtgttt ttcttccagg
     2521 gtgggttcgt ccacggtggg gtcgcgcaag gtggcgatca ggctcatctg cttggcttcc
     2581 cagcggtact cggccaggct ctcccagccg gcgcaggcgc ccagtgcgca gtcgcaggcg
     2641 cgaggcgcgg tggggcgggc gatggccgca cgcagttcag cggcagtctg gatgaatgtc
     2701 aagggtgaac tcctcaagtg gtcttcatgt ggccagggcc gcaaaaaagc ccgcccatgt
     2761 tgccacgggc gggctggatg tcaggcgtac aggcggatca gcgtgcgttg atcggcttga
     2821 cgtcgcgctg gacggaaccg ttgaacagct ggcgcggacg gccgatcttg tactcggggt
     2881 cgccgatcat ctcgttgagc tgggcgatcc agccgaccgt gcgggccagc gcgaagatcg
     2941 cggtgaacag cggcacgggg atgccgatgg cgcgctgcac gatgccggaa tagaagtcga
     3001 cgttggggta gagcttgcgc gaaacgaagt attcgtcttc cagggcaatc ttttccagcg
     3061 ccatggccag cttgaacagc gggtcgtttt ccagacccat ttcctgcagc acttccttgc
     3121 aggtttcctg catcagcttg gcgcgcgggt cgtagttctt gtacacgcgg tggccaaaac
     3181 ccatcagctt gacgccggag ttcttgtcct tgacctgctt gatgaactcg ccgatgttct
     3241 cgatgccgcc gttcttctgg atgtcgccca gcatgttgag ggccgcttcg ttggcgccgc
     3301 cgtgggccgg gccccagagg ca
//
LOCUS       DAIDFG010000337         3320 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_337_length_3320_cov_17.522818, whole genome shotgun sequence.
ACCESSION   DAIDFG010000337 DAIDFG010000000
VERSION     DAIDFG010000337.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_337_length_3320_cov_17.522818"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          565 a          981 c         1135 g          639 t
ORIGIN      
        1 gaagtcgtga atcgccgtct tgatctggtc gcccgccttc ttgtcggggc agaccgcgtg
       61 caccgtgtgc cccaccgcca gcagtgcctg aaacggcacc atggtttcat agtcttcgca
      121 atagtcaccg caaatcatca ggattttttt ggccgccatg tctgtctcct tgggttgctg
      181 agcaagttct cagccccgaa ttattcagcg catggcagtg gcctgggtgt tagcgggcta
      241 ctacgtgggg caggggcatg gcatgttggt gccgcctgcg atgagctact gcgtcaacag
      301 tgaattgatc agcagcaagt cttccggcgt cagcgtgccg ttggcctgac gcaggcgcag
      361 gccgccaatc agcacgttgt aacgcgcctg tgcgaggtcg cgtttggtct ggaacagctg
      421 gctttgtgca ttgagcacgt cgatgttgat gcgcacgccg acctggtagc ccagcttgtt
      481 ggcgtcgagc gcgctctggc tggacgcttc ggcggcctcc agcgccttga cctggccctg
      541 gcccgacaac acgccgaagt aggcggtgcg cgtgctcagg gcaacgccgc gtttggcgcc
      601 ttcgaggtca gtgcgggctt tttcttcgag tgacagcgtt tcgcggatgc ggttttgcgt
      661 ggcgaagccg gcgaacagcg gcaggttcag cgccaggccg accgtggtgc tgttggtgcg
      721 gtagtccagc gggctggtgg cactgccctg ggggttgcgc tgcacgccgt agctggcggt
      781 caggtccagc gtgggtttgt ggccggcttc ggccttgtcg atttcgagct tggcgacttc
      841 caggttggcc tgggcctggc gcaccgtcgg gtgcaggtct tcggattgcc ggacccaggt
      901 gttcacgtcg gcgggcacca gcggcggcag ctcgacgggc accagcaggc ctttgggctg
      961 ggcgtcggtt ttgccgacca gctggtcgag cgcgattttc ttgacgcgca agtcgttttc
     1021 agcggcaatt tcctgcgcgg ttaccaggtc gaaacgcgcc tgcgcctcgc gcgagtcggt
     1081 gacggtggag gtgccgacct cgaagttgcg tttggcagaa gccaactgct cgcccaccgc
     1141 ggctttttgc gccctgacaa aagccagcgt gtcctgcgcg gccagcacat cgaaatacgc
     1201 ctggctggtg cgcacgatca ggtcctgctc ggcggcttcg agctgggccc tggacagcac
     1261 cacttgctgc ttgccctgct cgtaggtggc ccagttgccg ggccggtaca gtggctgcga
     1321 ggcgttcagc gaggccgttt gcgtcgggaa gctgcggtcg gcgaccgggt tgttgttttc
     1381 aaaattggtg cgcgaggcac ccatcgacag cccggcggtt ggcaaaatgc cggccttggc
     1441 ctgctcggcc ttggccaggt tggcctcgta cagcgaacgt gccgactggt aggctgcatc
     1501 gtactcgcgc gccgaacggt acagctccac caaattctgc gcctggacgg ccggcgcaaa
     1561 agccgccagc acggtaccta acgcaagaga caggggcagc agggccggtt ttttgaagag
     1621 cgttttcatg gtgtgtcctg atgaaatgtg aagtcggtga agagggcggg ccaggggtca
     1681 gtagcgtggg acggaggcat cgcgtagcac ggaccacgcg ttgatgccgc cctgcagatt
     1741 gaccacttgc gtgaaaccgt tttgcgccag aaagttggcc acctgctggc tgcgcgcccc
     1801 gtggtgacac aggcaggcca ccggctggtc ggccggcagt tcttccttga gatcaaaaat
     1861 ccgcgcgggg acggcgcgca tcggaatgtg cagcagcctg aaaccgtctt ccttgaccga
     1921 cgctgtctgc acttcccacg gctcccgcac atcgagcacg atgggcgcgg ggccgccggc
     1981 cggcaggctg gcaagccagg cggtgaagtc ggcgggggac agttggtcaa tcatcgggac
     2041 ggctcgggtt aaaagttgaa tttcgagggt tcagggaagt tgcgcaggcg tggagcaacc
     2101 gtgtcccagc cctgcgtggt gcggaaggcg gcgtcgccga cgcgggtgac cagtgtggcg
     2161 cgcatgacag gctcaaaacc cacgatggcg ctgagccggc cgccgatttt cagcatcgcc
     2221 agcagcgcgg gcggcacttc ggcgaccgag ccgctgagca caatcgcgtc gaaggggccg
     2281 ctcagcgggt cgttgccgga gacggcctgg ccgagtttgg cggcgccgtc gccggtgcgc
     2341 acttcagcgt tgtagacgcc ggctttcttc aggttgtcgc gggccagctg ggccaggtcg
     2401 gggtcgatct cgagcgtgat gacttgctgg gcgcggtggg ccagcagggc cgccatgtag
     2461 ccggagcccg taccgatctc gagcactttt tcatgttttt gcaccgccag gtcctgcagg
     2521 acgcgcgcct cgaccttggg cgccagcatg cactggccgg gtttggagcc cagcgggatc
     2581 tccatgtcgg caaaagccag ggcccgcatc gcggtcggca caaagtcttc gcgcttgacc
     2641 acggccagca gggaaagcac ctggctgtcc agcacttccc aggggcggat ttgctgctcg
     2701 atcatgttga aacgggcttg ttcgtagttc atggtgtggt ctgtaataaa cggtgggact
     2761 gtcgggggaa acccatcatt ttagttggga ggcaccccca ggggcgggca gggcacgccg
     2821 gccggtgaag attcgtttga accattgcgc cagatcatcc aggtagcaat aggtcaccgg
     2881 caccaccacc agcgtcaaca gcgaggaggt gatgacgccg ccaatcacgg cctggcccat
     2941 gggggcgcgc tgctccgagc cttcggtcag tgcaaaggcc agtggcacca tgccgaaaat
     3001 catggccagc gtggtcatca gaatcgggcg caggcgtacg cgggcggccg tcaagagggc
     3061 ttcgctgcgt tccatgcctt cgtcccgcat gcggatagca aaatcgacca gcaaaatcgc
     3121 gtttttcgtg accaggccca tcagcatcac cacgccgatg atggaaaaca tcgacagcgt
     3181 cgagcgaaac agcagtagcg ccagcaccac gccgatcagc gtgagcggca gcgaggtcat
     3241 cagggccatg ggctgcagaa agctcttgaa ctggctggcc aggatcatgt agatgaacag
     3301 gatcgccatg accagcgcag
//
LOCUS       DAIDFG010000338         3314 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_338_length_3314_cov_19.626879, whole genome shotgun sequence.
ACCESSION   DAIDFG010000338 DAIDFG010000000
VERSION     DAIDFG010000338.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_338_length_3314_cov_19.626879"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          599 a          981 c         1008 g          726 t
ORIGIN      
        1 gattgggccg cctccaggcg ccacatcatc caggcctcaa agctctgctc ctcgggatga
       61 atgagcagat gggccagtcc ttcgggcttt tcctgcccaa gccaggttct cagcaaggac
      121 agcgcgccga cgttctttgg cgtccagccc ttatccagca gcagccgcac ccgggcgccc
      181 atctcaccat tttccatggc atctagcagc tcctggcgcg ccggctcggg caaggaaaac
      241 agcctggtgg caaaggtttc ggcctggaac cgggcaaatt cggtcttcat gcgatcgctg
      301 cgttccaagg ccaggcgctg tgcctcggct gcgcgtgcct cttcctcaag cttgtcgtcc
      361 agcaaatcat ccgcgccggc gccgcccgcc atgttggaga ggatcccccc aaagtaggcc
      421 ttgtggctgg tgatcggctt gccggacgcc ttgagccggt cctcggccga tttcagccgg
      481 ttcagcgact cggtcaccac ttcatatgag tgggcctggc tcatgttctc gatctcctgg
      541 tcggtaaagc ccagcgattt gagtgtcttg agcaggtctt ctgaccaggt gagcggcagc
      601 cccagcgagg cctgggactt ggggatgaac ttgaactgca gcctggtgac gcgtttcccg
      661 ttgaaaagct cgagcagttt gagggtgtgg ttcagcgccg gcgactcgtt cacccgccgg
      721 atggcatcca ggagaattcg ccgcttgaag tcgccatagc ccgtggctcg cttgctgccg
      781 tccttttcct ccttgacgaa ccgggacggg ccgaccagca gctctatcca ggtgtgggtc
      841 ggcagcggtg ccgtcacctt ggcctgggtg ccgatgtacc gccagcagtt ctggtacagc
      901 gcgtagctgg cggccgtgct caggctttcc attgcctgca ggctcaacct ggcccagatg
      961 gtcggttcca gcacgatctc caggatggcc gggtcgatgg aaaagcggat ctggccacgc
     1021 cggtggttgc gcccataacc cagagacgac aggaaatggc tcttgaactc ccaggtcgtg
     1081 gtgttgtcct cccccaccac gttccacttg agaaccatct cgaacagctt gtccagatcc
     1141 ttgtagattc gttcgtagtt cttgcctggg atgttggcca ggcgggccag gtcagtgatg
     1201 ctgacctcga acaacggtga gctccgctcc tgctgcatct gggtaagctg caagcgatcg
     1261 cgcttgcgat aatcgagttg ggccaccaga atgcaggcat cgaacaggcg ccggctgttc
     1321 aggttctggg catgctcggc gcgcgaaggc accgcatgca agatggctac cggcttgcgg
     1381 aaggtgttga gcgtctcggg gatgtgcagg tcgccaggcg ggaaaagcga gggctgatca
     1441 gcctcgtggt gccgcatgtc gaaaagccgg ccgtcctctg ccggtgggtc cagttcgggt
     1501 atggtggcag gatcggggtc atctttcaca tcgcaataat aaccgatggc aatagccggc
     1561 agtgtgttgc catcatcact tccggcctgg tgaaagtatg cagaactgct gccttcagcc
     1621 accagggatg ccttcgtgag tatgcatttt ggttagcttt tgcatgccga cgccacacaa
     1681 agtatgcaat tcggtcggat ttgttcgata agtccttgat tttattgagc aaaaagcccg
     1741 tgaaactctt gaatgtttac ggaatgactt gaaaccaacg gaacagggct gctcgaaaat
     1801 gcccggaact gcagtaattg gctgcaatgt gggccgcaaa ccgtcggttt tgtcttcaat
     1861 atggagatcg ggagccaata ccgagagccg gcccccacct tcaaggctga aaaccgtcag
     1921 ttctgcatac tttcacgcca gcatgcggtg tgcagtccag gggaaaacag tgaatcaggc
     1981 cccaaaacga gctgctgcat atctggaaag agtatgtgcg tatgcttttc acacgcgttg
     2041 ctcagcctgt acactgtctg cgtgaacgac gactctccac agtttaagct caatgcgctg
     2101 gtcggctctg ccctgcgcgg gctggtccag tccggcgctg atgaccaggc gatcggcgct
     2161 ttcgcggccg agcaccgtga gcgggttgcg caaatcctcg gcatggtagc ggcaccggtc
     2221 gctccggtgg atctgcaggc cgtgatcgag tccgcggtcg cccaggggat tgaaaaggcg
     2281 caaagccagg gccgtctggg caagccgcgg cgccaggccg gcgctgcccg gcgggtctat
     2341 gtcgtggtcg ccggcgccag aacctcggtc accatcccaa gtgcactgtt tgaggaagtt
     2401 gcggctgccg gcggtggtgc caaagaagca cggcgcctgg ctgagcagta tgcaaatcag
     2461 gcgccggatg agggaaatcg gtcaggttgg acggttgagc gtctcagggg gcacctcgca
     2521 ttgcacgatg ccccaggggc tgcacagccc cgccactgaa tttaggttta ctcgttcagg
     2581 ccgaagcggg tacgcagctc ggtcaccagg gcggcgcgct ccttcggatc cgtgaagctg
     2641 acctcgaatg cgacacgacc gttgtcccac tccttgagcg agccgatctg cacatctccg
     2701 gagcggatct tgtactgacg cgagttttct ttcttgcggc ggggcgtgcc cgccccttcc
     2761 gtgcgctcgc gcagcgcagc cacttgccgt gaagtcaggt cttcgttgat caccttgtcg
     2821 accaaggcca gcaactcggg ttcctgcagt ttcttgttca gcatggcaat ctcatacccg
     2881 gtcgacaccc cgaacttgtc cggcgccgtg ctcatgcgtt caacgacgga tttgggcaga
     2941 tccatcaggg cgagggtctt gttgatcgtt gccttggaca tgcccagcat ggtcgcgatc
     3001 tcgacctcgc tgtcgaccac cttgtcgtca atcagcttct tccaggcgaa ggcgttgtcg
     3061 agggtggtct ggcctgcgcg ctcctcgttg atgcgccagc tcatcatgta catctcgacg
     3121 ttggccatcg gcgggataac gtccagttcc atctcggtga tgttcgcctc gaccgcgccc
     3181 ttcttccgat aaagtccgtc gatgacgatg tagtcgccgg gaatcgttgg gtgcggaacg
     3241 gccttggcgg ccaccatctg gccatttttc tttaaagatt cggcacgttc gcggatcttt
     3301 tcctggctat aaat
//
LOCUS       DAIDFG010000339         3258 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_339_length_3258_cov_19.332813, whole genome shotgun sequence.
ACCESSION   DAIDFG010000339 DAIDFG010000000
VERSION     DAIDFG010000339.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_339_length_3258_cov_19.332813"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          634 a         1044 c         1017 g          563 t
ORIGIN      
        1 catggtcaac tgcaaccccg agaccgtctc gaccgactac gacaccagcg accgcctgta
       61 tttcgagccg ctgacgctgg aagacgtgct ggaaattgtc gacaaggaaa aaccgctggg
      121 cgtgatcgtc cagtatggtg gccagacgcc cttgaagctg gcgctggacc tggagaaaaa
      181 cggcgtcccc atcatcggca cctcgcccga catgatcgac gcggccgaag accgcgagcg
      241 cttccagaag ctgctgcatg aactgaaatt gcgccagccg cccaatgcca cggcccgcac
      301 cgagcccgaa gcgctggaaa aagccgccgc cctcggctac ccgctggtgg tgcgccccag
      361 ctacgtgctc ggcggccgcg cgatggaaat tgtgcatgag caacgtgacc tcgagcgcta
      421 catgcgcgaa gccgtcaagg tcagccatga ctcgccggtg ctgctggacc gcttcctcaa
      481 cgacgcgatc gaatgtgacg tcgactgcat ccgcgattcc gaaggtgtga ccttcattgg
      541 cggcgtgatg gaacacatcg agcaggccgg cgtgcacagc ggcgactccg cctgttcgct
      601 gccgccgtat tcgctgagcg cggagacagt gaccgagatc aagcgccaga ccgcggccat
      661 ggcccaggcc ctgaacgtgg tgggtctcat gaacgtgcag ttcgcgatcc agcacgtgga
      721 cggcaaggac gtgatctacg tgctcgaggt caacccgcgt gcctcgcgca ccgtgccttt
      781 tgtcagcaag gcaaccggca tccagctggc caaggtcgcg gcacgctgca tggtcggcca
      841 gacgctggcc tcgcaaggca tcagcaagga agtcacgcca ccgtatttca gtgtcaagga
      901 agccgtcttc cccttcgtca agttccccgg cgtggacacc attctcggcc ccgagatgaa
      961 gtccaccggt gaggtgatgg gcgtcggcaa aaccttcggc gaggcctttg tgaagtcgca
     1021 gctcggtgcc ggcaccaagc tgccgacctc cggcaaggtc ttcatgacgg tcaagaacaa
     1081 cgacaagccg cgcgccgtgg aagtggcgcg tgcgctggcg gcgctgaagt tcgagattgt
     1141 cgccaccaag ggcacggctg cggcgatcaa cgctgccggc attccctgcg gcgtggtcaa
     1201 caaggtcact gaaggccggc cgcacgtggt ggacatgatc aagaacaacg agattgcgct
     1261 ggtcatcaac accgtggaag aacgccgcaa tgcgatcgcc gactcgcggc agatccgcac
     1321 ctcggcgctg ctggcccggg tcaccaccta cacgaccatc gccggcgccg aagcggcggt
     1381 ggagggcatg aaatacctgg acaacctgac ggtttattcg gttcaggaac tccattccca
     1441 actggcctga gctcgggaca actaccgagc ccgcgccggg gcatttgcat taaactcgca
     1501 ttaggacaaa catgttaaga cgccgccgcc aggtgactgg tggcggtttc gcttttggag
     1561 gactcaaacc atggcaacca ttccgattac aaaaaagggc gcggaaaagc tcaaggccga
     1621 actgcaccag ctcaagaccg tggatcgccc ctgggtgatc aactcgattt ccgaagcgcg
     1681 ggcccagggt gacctcagcg agaacgccga gtacgacgcc gccaaggacc gccagggctt
     1741 catcgaaggc cgcatccagg aaatcgaagg caagctgtcg gccgcgcaga tcatcgatcc
     1801 gtccgaggtc gatgccggcg gcaaggtggt gttcggctcc acgatcgagt tgcaggacga
     1861 ggactccggc gacaaggtga cctaccagat cgtcggcgaa gacgaggccg acatcaagct
     1921 gggcctggtc aacatcagct cgccgattgc acgcgcgctg atcggcaagg acgagggcga
     1981 caccgccgaa gtgcaggcac ccggtggcat caaacgctac gagatcatcg cggtgatgta
     2041 catctaggct gccgcatgaa accacgactg agcatcctgc ttgccgccct ctggtggggc
     2101 agcctgacgg gtctgggttt tgtcgtggtg ccgatgttgt tcatccacct gcccagcccc
     2161 gccgcggccg gcgccatggc cgcgaaactg ttcacggccc agacgtggct cagcgtggcc
     2221 tgcaccatgc tcctgctgct ggccttgaat aaaaaagagg ctccagccct ttccccctct
     2281 gcgcaagcag ctatgaaatt catagtggcc ggcctgctgc tggcggtgct ggtggagttc
     2341 ggcgtggcgc cgcgcattgt caatgcacgc gttgacggcg gtaacctcag gctctggcat
     2401 ggcctgggca gcgcgatgta cctggcccaa tggctggctg ccggctggac gctgtggtgc
     2461 ctgagccgga cgacaaaagc tgccgacgcc gagacctgaa gctccagccc gcggtttcta
     2521 gctgcgctcc gccgcttcca gggtattgac cagcaacatc gtgatggtca tggggcccac
     2581 accgccgggc accggtgtga tccagccggc gacttccttc acgccgtcga aatccacatc
     2641 gccgcagagt ttgccttcgt cattgcggtt catgccgacg tcaatcacga ccgcgccggg
     2701 tttgaccatg tcggccgtca gcacattgcg cttgcccacg gcggcgacca ccacgtcggc
     2761 ctgaagcgtc agggccttga ggttttgcgt gccgctgtgg cagatcgtga ccgtggcatt
     2821 tttctgcagc agcatcagcg ccatcggttt gccgacgatg ttgctgcggc ctatcaccac
     2881 cgcatgtttg cccttcaggt cgtagttgat gctctcgagc atcttcatgc agccataggg
     2941 cgtgcagggc cagaagccgg gctggccgac catcagcgca ccggcgctgg cgacgtggaa
     3001 accatcgacg tccttggccg gcgaaatcgc ctcgatcacc ttgtgcgcat cgatgtgggc
     3061 gggcagggga agctggacca ggatgccgtg gatggccggg tcgcggttca gggcatccac
     3121 gcgggcaagc agctcggctt cgcccagcgt cgccgggtaa cgctccagca ccgaatgcag
     3181 gccgctgtcc tcacaggcct tgaccttgtt gcgcacatac acctggctgg ccgggttctc
     3241 gccgaccagc accacggc
//
LOCUS       DAIDFG010000340         3228 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_340_length_3228_cov_15.020485, whole genome shotgun sequence.
ACCESSION   DAIDFG010000340 DAIDFG010000000
VERSION     DAIDFG010000340.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_340_length_3228_cov_15.020485"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          562 a         1038 c         1071 g          557 t
ORIGIN      
        1 caggccgccc gagcccggct gatcgcgcgc cgcaggggac ttgttcagcg cttcctttat
       61 gccctgccgg cgttgtgtac gctgccatgg cctaccatgg caacacctcc tgaaccttac
      121 cggattttcc ccatgatcac tgtgcatcac ctcaaccact cccgctcgca acgcatcctc
      181 tggctgctgg aagaactggg cgttgactac gagatccagc gctacgagcg tgaccccaag
      241 accatgctgg cacccgcgac cttgcgtgct gtgcatccgc tgggcaagtc gccggtcatc
      301 accgacggtg aactgacctt ggccgagtcc ggcgccatca ttgattacct gatcgagcgt
      361 tatggcgacg gaaaattctc tccgccagcg ggaacaccgg cgcgcctgcg ctacacctac
      421 tggctgcatt acgccgaagg ttcggccatg tcgccgttgc tgctcaagct ggtgttcgac
      481 aagatagaaa ccacgcccat gccgtttttt gccaaaccga tcgccaaggc gatcagcgcc
      541 aaaaccaagg cggctttcat cgagccgcag atcacacagc acctggacta cctcgagggg
      601 gagctgggaa agagcacctg gtttgcaggt gaagcgttca gcggtgcgga tatccagctg
      661 agttttgtgc tggaggcggc ggcggcgcgc ggcgggctgg atgccagccg tccgaagctg
      721 atggctttcc tgaagcgcat tcacgcgcgg ccggcttaca agcgggccat cgcacgcggc
      781 ggggaataca ccctgctgca ctgaattttc aggtctttta tgccctcagc cattgaggga
      841 cgggcgcaag cagctatcga ttcgatagcg aattcaatag cgttttctgc gccggtgcgg
      901 tttcagcgaa agccgaagta agacaggatg aagccgatga tgacgatcag gccgacgatg
      961 taaatgatgg tgttcatgtg gttcctctgg gagttttccg ggtgcaccgg gatgtacacc
     1021 ttctgtccca gatcatgaac gcggcggcgg cctccggctg taggctgccc ctgcctgcgg
     1081 ccgtcagaca agggctacag cgccccggcc gcggccccag ttcaacttcg gcgcgtcagg
     1141 ccttgttttt catggaacag gtgttccagc cgaacagggc gtaaggcggg caccagccga
     1201 tggcaccggt ggccagcgga acgacgccta tccagcccca caggccgacc gtgccggtgg
     1261 cggccagcgc tatcaggacc aggccgataa cgatgcgaag gatgcggtcg atgccgccga
     1321 cgttggattt catggtgagc tcctttgaaa aaggggaagg actgatgacc gcattaggcg
     1381 ccctggcacg gcccgggtca gtgacctggg tcacgcagca gccttgcggg ccgccccggg
     1441 gctcaggcgg gcaggtccgc ggccatggcg cgtaatgcgg cgctgtcgcg gatgtgaatg
     1501 tgctcgcgtg cgagttcgac ccagccttca cgctcaaagc ggcgcaacag gcgcgagacg
     1561 atttcacgca cggtgccgag ttcgtcggcg agcgtctggt gcgttagcgc caggtcctgg
     1621 ccgcggccca gcagcgcggc cgccagccgc tggtccagcc gctggaaggc gatggcatcg
     1681 atcagcccgg tgaggtcggc catgcgttcg gcaaacaggc ccagcacctc gttgcggaaa
     1741 accggggttt ccagccagcg ggcgaacacc ggcggggcaa tcagcagcag ggtggtgggc
     1801 cgggtggtca cgccctgggc cgacagcggc tggctgcgaa acaggcaggc gctggagacc
     1861 aggcacaact cgccggggac cacgcggtag agctccagcg agcgcccgtc gccggaatgg
     1921 cgcgagacct tgacctcgcc gctgagcacc agcggaaagc cctggcaggg cgcgtgttcg
     1981 tcaaacagca cggtgcctgc cggcacgctg agcgggccgg cgtcgggccc cagctcggcc
     2041 agcgtgggca cgacttcggc cagggcgggg tacaaggcga gggggtcgtt catggggctg
     2101 atgctcgttt ccggggtggc cggggtggtg gcaggctttg ccggcgtcac tatgctacca
     2161 aatcaatagc tgcttgctcc cgtccctgtt gggctggagg ccaaaatcat tcaaatctcc
     2221 gccgcctcca ccagaaaacg cacacgtttg atgccctgcc attcgtcggc atcgagccgg
     2281 aaggcgagtt tgacgcgcgc cggcagcgac tcggtgtggc cgaaccagat gccgtccacc
     2341 ggctggccct ggtgcttgag cttgagcgac aggtgttttt cgccgaccag ccgctgcgag
     2401 atgacctcga cttcctcgct gaaagtcggt gccgcaaaac cctggcccca gacctcttta
     2461 tgtagcgtgt ccaccaggtc ggcgcgccgg tactcgggcg ccagcggacc gtcggtgtcg
     2521 atgcggcgca gcaaggtggc ggcgtccagc cactcgtgcg ccaccagttg cagcgcctgc
     2581 tcgaacacct cgaagtgttc ctcggcaatc gtgcagccgg ccgccatcgc atggccgccg
     2641 aaacgcagca acacgccggg gtggcgcttg gccaccaggt ccagcgcatc gcgcagatga
     2701 aagcccgcaa tcgagcggcc cgaacccttg agctcgtgtt ccttgcccgg cgcctggctg
     2761 gcggcaaaca caaaggtcgg gcggtgcagc ttgtccttga tgcgcgaggc gacgatgccg
     2821 accacacctt cgtggaagtc cgggtcgaag atggcgatgg ccggcggcgg cccatattcc
     2881 ccgtgagcat cttcgtcgat cattgcatcg gcggccaact gggcctgctc gcgcatgccg
     2941 gcctcgatgt cgcgccgctc gcggttgatg ccgtccagcg tttttgccag ttcgtcggcg
     3001 cggcccgcat cgtcggtcag caggcactcg atgcccagcg tcatgtcgct gagccggccg
     3061 gcggcgttga tgcgcgggcc gaccgcaaaa ccaaagtcga aactggtcgc caccgccgcg
     3121 acgcgaccgg cggcggtgaa cagcgccgcc aggccggccg gcagggcgcc gctgcgtatg
     3181 cgcttgaggc cttgcgccac caggcggcgg ttgttggcgt cgagccgg
//
LOCUS       DAIDFG010000341         3223 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_341_length_3223_cov_15.798611, whole genome shotgun sequence.
ACCESSION   DAIDFG010000341 DAIDFG010000000
VERSION     DAIDFG010000341.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_341_length_3223_cov_15.798611"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          542 a         1011 c         1075 g          595 t
ORIGIN      
        1 agcggatccg ggttgtcctt gagtgtcagc gtcgggcagc ccgacaggcg gatgcacgcg
       61 tggtcgccgt tgcagatgtc ttcatcgaca ccatatttca cgcgcaccac gcgtttgcct
      121 tttttgagga gaccggccag ccagggcttg atgcggcgtt ggcgttcgag ctggcattcg
      181 ccctcggcaa tgatgacctt caggccgttg aagtcggtcg tgaaggcctc gttgagcgtc
      241 tggcgcatct cctcgacatg gtaggtgtgc accgtgcgca gccactggat gcccaggccc
      301 tgcagggtgt tttcaatcgt cttgttggtg tcggtcaggc tttgcagctt gtcgctggcg
      361 ttgaatttcg cgtcgtcgct cggggtggag atgatgtcct gcgtgccggt ggccgaggtg
      421 tagccgttct tgaagatcag cagcaccgcg tcgtcgccgt tgaacagcgc cgactgcaca
      481 ccggtcagca ggccgttgtg ccagaaaccg ccgtccccca tgatggacag cgtgcgccgc
      541 gccatcatcg gcgagacgcc ggcgcggctg gccagactca tgccgtagcc gaggatggag
      601 tggccgaaag agaagggcgc gaaggtgccg aaggcgtggc agccgatgtc ggccgcgatg
      661 tgcaccggcc cgacgtcctg ttgcgccagc ttgagcgccg agaacaccgg acgctccggg
      721 cagccgatgc aaaagcccgg tggccggacc ggcaaagcct gcttcagttg cgcctcgatc
      781 tcgcggccga ccgcgctgcg gcgctcggca ttgcccctga gccaggcggt ggccgacgcc
      841 gtgtccacgt cggtggcgta acgcgcgaca aaggcggcca ggccgctggc gaccacctcc
      901 accgaatatt cgccgcccga gggcaacatg tccttgccgt gcagcggcgt gttgatgtcg
      961 cgccggcgta aaaaggtggc gatctcctgc tcgatgtact cgggctggcc ttcctcgacc
     1021 accagcaccg cgcgcttgcc gagtgcaaag ccggccacct gctcgggcac cagcgggtag
     1081 gtgacattga gcacgaggat gggaatgtcg gtctggccaa acgcgtcgca caggccgaac
     1141 tgctgtagcg cgcggacgag tgagttgtag atgccgccct gcacgatgat gccgaggtcc
     1201 tggcgcggtc cgtccatcag ctcgttgagc tgctgctcga tgatgtagcg gcgcgccgcc
     1261 gggatgcgct cctcgaactt gagtttttcg tgccggaagg tcaccggcgg atgcgcgagt
     1321 ttggcgtagt caaaggcggc gggttcttcc atcagctggc gcgtcgagat cgccggcggg
     1381 cgattgtcct tgcattcgaa gctgccgcgc acatggcagg cgcgtatgcg cagttccagg
     1441 atggccggca tgttggaggc ctccgagatg cgaaagccgt gttcgaccat gtccaccatc
     1501 ttgccgaggt cgggccgcgg gtccagcagg cacatggacg atttgagcgc gtaggcatgg
     1561 gtgcgctcct ggatcacgct ggcgccctcg ccatagtcct cgccgaccac gatcagcgcg
     1621 ccgccggtga cgccgggcga cgagagattg gacagggcgt cggccgcgac gttggtgccc
     1681 actatcgatt tccaggtcac ggcgccgcgc agcgggtaat ggatggatgc gcccagcatg
     1741 gccgcggccg atgcttcgtt ggagcaggct tcgacatgca cgccgagttc gtccatgtag
     1801 ggcttggcct gcaccatgac gtcgagcagg tgggacaccg gtgcgccctg gtagccgccg
     1861 acgtaggcga caccggactg gagcagggcc ttggtgatcg cgaggatgcc ctcgccgtgg
     1921 aaagtctcgc ccgcgctcat gcgcagtgac tcgatttcct tgctgaatga aacttccaag
     1981 gctgtctccg ggttttattt tcgtgggaag cagcgagttt gggccccatt cgactatcca
     2041 tcaataaaat aacaagacta tctggtattc attcaatgca tataaaagac atcgacctga
     2101 acctgctccg gctgttcgac gcggtctacc gcacgcgcaa cgtcagccgc gcggccgagt
     2161 tgatggacct gacccagccc gccgccagcc agggcctgac gcggctgcgg ctgctgatca
     2221 aggacccgct gtttgtgcgc gccggcggcg gcgtgcagcc cacgcccaag gccgacaggc
     2281 tggccgacgc ggtgcgcagc gcgctggcca cgctggagca ggcgctcaat gaatcggccc
     2341 tgttcgagcc gctgcgctcg cgcaaggtgt ttcgcatcca catgagcgac atcggcgagg
     2401 gccgcttcct gcccgagctg atggcggcgc tgcgcgaact cgcacccggg gtgcgcattg
     2461 aaacctcccc gcgggccagc aacgatgtcg ccccggcgct ggacagcggg cgcatcgact
     2521 ttgcgttcgg ctttttgccg gcggtgaaag acacgcagcg cgtgcagctg ctgaaagacc
     2581 gctacatcgt gctgctgcgc gaaggccacc cttttgcgcg gcgccggcgc agcggccagg
     2641 cgctgctcga cgccctgcag caactggagt ttgtggcggt gcgcacgcac tccgacacct
     2701 tgcgcattct gcagatgctg cagctggaag agcgcctgcg gctgacgacc gaacacttca
     2761 tggtgctgcc gtccatcgtc aaggccaccg acctcgcagt tgtgatgccg cgcaacatcg
     2821 ccgccatttt tgcggccggc ggcggttaca ccgtcatcga gccgccgttt ccgctgcgcg
     2881 acttcacggt gtgcctgcac tggagcaagc gctttgaagc ggatcccggc aaccgctggc
     2941 tgcgcggggt gattgaggac ctgttcaggg agtagtgcgg ggccgcagcc gtcaaagaag
     3001 ttccagggta cccgggtccg gtacaccgga gagatatttt gccaatgccc ggccgaacag
     3061 ggattcctgc ggcgcctcct gcgcaaacag cgtcaggcag gcgtggaact tgaggccggc
     3121 cgtcgcacca aaaattttca acgcggattt ttcctcgatt accagtacgc gttcaataca
     3181 ttgcctgagc cgcggcccag gaacgggata ttgccaacag gcc
//
LOCUS       DAIDFG010000342         3214 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_342_length_3214_cov_18.151630, whole genome shotgun sequence.
ACCESSION   DAIDFG010000342 DAIDFG010000000
VERSION     DAIDFG010000342.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_342_length_3214_cov_18.151630"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          537 a         1149 c         1014 g          514 t
ORIGIN      
        1 ccgcggaacc ggctttgcca ggccgcaggc gcagcgcccc ctcggggggc agcgaagtac
       61 acgaagtgac gagcgtgggg gctccatgac agacccgttg cacggcgtca cgctggaagc
      121 catggtcacc gccctgtcgg accactatgg gtgggaggaa ctcgggcagc gcatcgcgat
      181 ccgctgtttc accttcgaac ccagcgtgcc gtccagcctg aagttcctgc gcaagacccc
      241 gtgggcccgg gacaaggtcg aaagccttta cctcttcatg ctgcgcgagc agcggcgtgc
      301 cgccccgacc gtgcacaact tcccgccacc caaaaactaa gctgccatcg ggttggcgaa
      361 cagcagcatc atggccacgc cgacaccgat caggaagttg gcatcgatca ggcccgccag
      421 caaaaacatc ttgacctgca gcggctccat cagttcgggc tggcgcaccg acccttcgat
      481 gaaacggccg cccatcacgc ctatgcccag gcaggcgcca atcgcgccca ggccgatcat
      541 caaaccacat gcaatcgaaa tggcacctgc atcactcatg atttttctcc ttcaaggtac
      601 ttgctgatgg gtccatcatg aatgcaggtc ggccggagat ccatgacctt gcaggtaatg
      661 acggtgcgag tggcttcgac ctcgtccatg ccgatgccgc cgtgctggtg ctggtcaaac
      721 ccgccggcct gctggcggtg ccgggcaagg gcgaggacaa gcaggactgc ctgagcgcac
      781 gcgtccaggc aagctacccc gacgcgctgg tggtgcaccg gctggacatg gcgacctcgg
      841 gcctgatgtt gatggcgcgc agcatcagca tccagcgtgc cctgggcgcg ctgttcgaac
      901 gccgcgagat ccacaaacgt tatatcgccg tggtcgatgg ccggcccacg gccacacccg
      961 gtgccggtcc cgggggctgg agcctgatcg acctgcccat cgtggtggac tggccgcgcc
     1021 ggccgctgcg catcatcgac gccgaacgcg gaaaggccag ccagacgcgc tggcgggccg
     1081 tggcgtttga cgcgcccagc caaagcaccc gcgtggaact cgagcccgtc accggccgct
     1141 cgcatcagtt gcgtgtgcac ctgcaggcac tgggccaccc gattctgggc gacaggctgt
     1201 atgcgcccga ggacgtgcag gcccgcgcgc cccggctgct gctgcacgcc tgcgcgctgg
     1261 gttttgtgca cccggtcagc ggcatggccc tgcagttcga cagcccggcg ccgttctgac
     1321 cagggcgccg ggccggaact tcagcgcctg cttcgcatcc catgctgatc accggagacc
     1381 gtcgatgcgc cctgcctcac cacccctttc ccccgccgcc gtgctgcgcc gccgcaccgt
     1441 ggctgccgcc ctggtggcag cgcctgtgct gtggctgggc ccgcgcgccc agccggccgc
     1501 cagccgccgc gccacaccgg cgcaaaccga aggaccgtat tacccggtgg tcctgccccg
     1561 ggacagcgac catgacctgc tgcgcaacgg ggcgctcaat taccccgaag ggcaaagcgc
     1621 ctggatcgag ggcagcgtca gcgacctggc cggcaaaccc gtggccggcg cgcaggtcga
     1681 gatctggcaa tgcgaccacg ctggccacta ccatcacccc ggtgacggcg accgggccga
     1741 ccgccgcttc cagggctttg gccgggtgac agtggatgcc gaaggccgct accgctttcg
     1801 caccctgcgc ccggtggcgt atgccgggcg cacaccgcac atccacgtca aggtcaaact
     1861 cggacagcgg gaactgctga ccacccagct gtatgtggcc ggcgaccccg gcaatccgcg
     1921 cgacttcctg tggcgcagcc tgggcgaggc cgaccgggct gccctgaccg tgccctttgc
     1981 gccgggcagc gacggcctgc aggcccgctt tcccatcact ctggccgcct gaatcgccgg
     2041 gacacggaac ggcgtcaccg tttgtcggta acattgccag ccatgaccca acacctattt
     2101 atcctgaccg gcgcctcccg cggcatgggc ctggccatgg cgcgcaagct gctcatgccg
     2161 ggcaacacac ttctgtgcat ttcacgcaag gccaatgaga cgctggccgc gcaggccaaa
     2221 atcgccggca cgccgctcct gcagtggacg ctggatctgg cgcgaagcgc ggacgccagt
     2281 gcgcggctcg aacaatggct gcgcgagcag gcccacgccg gtttttccag cgccgtgctg
     2341 atcaacaatg ccggcatgat tccgcgcatc ggcccgctca gcgacgccga cgccgacgac
     2401 ctgcgcctgg ggctgcgtgt cggcctggaa gcacccatgc agctgaccgc ggcttttctg
     2461 cgcgccaccg aaagctggcc ggcgcggcgc aaggtgctca atatttcgtc gggactggga
     2521 cgcaaggcca tggcctcgca gtccatctat tgcgcggcca aggccggcat ggaccacttc
     2581 acgcgctgtg tggcgctcga agaagcgctc aagcccaacg gcgcagcggt ctgctcgctc
     2641 gcgcctggcg tgatcgacac cgacatgcag gtccagctac gcagcgccga cgcgtcggcc
     2701 tttcccgacc agcacaactt catcagcctc aaggacaagg gccagctcac gtcgcccgac
     2761 gaagccgccg cgcgcatcct ggcctacctg gcccggccgg actttggcag ccagccggtg
     2821 gcggatgtgc gcgactgatt ccagaacgct acggctttga tagctgctta cgctttcaag
     2881 ccattggctg agggcctatt tcgttcgcaa gctctcggcg ggaatttttt cggcgtcacg
     2941 ctgcgtctgt ggtccgcttt ggacagtagg cgcaactccc cacgccctcc cctcgccccg
     3001 ctgggcgatg gcggggagct tcatttggcc gcgtgtgcta ccgccgcgtg caaacatcac
     3061 ggccgcgtaa gaccgacatg acgcgcttcg cgcgtcatgt ctccccgcat ccgttgccgc
     3121 attcttgccc taccacccgt cgggctgggc cgagcagcgc agatggaggc gggatcaggg
     3181 ccgcagttgt ccgagcgcag cgagttctgc ggac
//
LOCUS       DAIDFG010000343         3205 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_343_length_3205_cov_18.069841, whole genome shotgun sequence.
ACCESSION   DAIDFG010000343 DAIDFG010000000
VERSION     DAIDFG010000343.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_343_length_3205_cov_18.069841"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          563 a          988 c          968 g          686 t
ORIGIN      
        1 aaagtcatct tcgcctcgtc attaggcact gttttcgaat ggtatgactt ttacctctac
       61 ggttcgctgg cagccatcat tgccaagcag ttcttcagcg gactcgatga aggctcggcc
      121 ttcatttttg ccctgctcgc gtttgcggcc ggtttcatcg tgcgtccgtt cggcgcactg
      181 gtgttcggtc gtctcggcga catgatcggc cgcaaataca ccttcctgat caccatcctg
      241 atcatgggcc tgtcgacctt catcgtcggc ctgctgccca actacgcaac catcggcgtc
      301 gcggcgcccg tgatcctgat tgcgctgcgc atgctgcagg gcctggctct cggtggcgaa
      361 tacggtggtg ccgccgtgta tgtggcagag cactcgcccc acggcaagcg cggtgcgtat
      421 acctcgtgga tccagaccac cgccacgctg ggcctgttcc tgtcgctgat ggtgattctc
      481 ggcacgcgta ccgtgctcgg cgaagcgacc tttgccgact ggggctggcg cgttcctttc
      541 ctggtgtcca tcctgctgct gggcgtgtcg gtgtacatcc gtctgagcat gaatgagtcg
      601 cccgccttca ccaaaatgaa ggccgaaggc aagacctcca aggcaccttt gtccgaatcc
      661 ttcggtcaat ggaaaaacct gaagatcgtc atcctggcgc tgatcggcct gaccgctggt
      721 caagcggtgg tctggtattc gggccagttc tatgcgctgt tcttcctgac acaatccctc
      781 aaggtggacg gcgccacagc caatattttt gtcgctgttt cgctgctgat cggcacgccc
      841 ttcttcgtcg tgttcggctc gctgtcggac aagatcggcc gcaagcccat catcatggcc
      901 ggctgcctgc tggccgcgct gacctacttc ccggtgttca ccgcgctgac caaagccgcc
      961 aaccctgatc ttgctgccgc acaggcaaag aacaaggtcg tcgtgacggc tgacgcgagc
     1021 gagtgttcgt tccagttcaa cccgaccggc acggcgaaat tcaccagctc ctgcgacatc
     1081 gccaagcagg tgctcgccgg cgcatcggtg agttatgaga acgcacccgg cgccgctggc
     1141 gaacctgcca ccatcaagat tggcgagacg gtcatcccca gctattcctc caagggcctt
     1201 cctgcggatg aagccaggaa aaaggacgcc gcgttcaaga agctggtggc tgacgacctg
     1261 aaggctgccg gttacccgac caaggccgat ccggccaagg tggacaagtt catggtgatt
     1321 gccatcctga cttacctggt gctgctcgtg accatggtct acggcccgat tgcggccatg
     1381 ctggtggagc tgttccccac gcgcatccgc tacacctcca tgagcctgcc gtaccacatt
     1441 ggcaatggct ggtttggcgg cttgctgccc accacggcct ttgccatcgt ggcgcagacc
     1501 ggcaacatgt acaacggctt gtggtatccg atcatcattg ccggcatgac cgtcgtgatt
     1561 ggcaccttgt tcatcaagga aaccaaggac gtcgacatct acgctgacga ctgatccccg
     1621 ggaatgtcag ccctgtggtt ctgcctgatt cccgggcaga accaccgaac aagtttttta
     1681 caaacccccg cgtcgccggg ggtttttttc tggagaaata acatgtggaa aattgcactc
     1741 gcattcgtgg tctttgccgg tctggccatc ttcatcctga gcaagggcgg agacattgac
     1801 atggggggcg aaaagcatgg catcgaggtc acgcatgaag ccccgccacc ggcatcggcc
     1861 gcttcagccg ccgcgtctgc cgcgtccgcg cctgcgcgtt agctcgccct ccgccccgga
     1921 cccgcaccat gatttatgcg aagaccgaga ccggacagca gtccttgaaa gacaggtccc
     1981 tgctctcgcc ccgacagcgc tcggccttca tcctgtttga cggcaagcgt tccgtttctc
     2041 aagtgctggg ggcgacagcc ggcctcggtg tcacgtcgga ggagctggtg caactggtgg
     2101 agctgggttt tctcacgccc agccagcagt ccgtcgacaa ggctgctgcc gcgaacgcgg
     2161 ccgccgtggt ggcccggcag gaggacagcg atgccagcaa tgaccgctac aagaaggcct
     2221 accccatcgc tgcgcagttg acgggggcgt tggggctcag gggtttccgg ttgaacctag
     2281 ccgtcgaagg tgcggtagat gtcgatgccc tgcttgcgtt gcttccgaaa atcagggaag
     2341 ccgtcggggc cgacaagtgc agcgagctgg aacaggccct caagggctga gggcccctgg
     2401 gcgttctagc gcctgaattt cccgtctgtg ccgatgatgg acaggtcggc gaaatcgagt
     2461 ccggtgtggt cgtcgaggga gtagttcact tccagcccgc caatgtcgaa cttgcgtatg
     2521 ctttccaggg cggcctggat tttttcacgc gtcggtttgg cgccggcccg tttcagccct
     2581 tcgaccagaa ccttggcagc ggcaaaccct tctagcatgg ccgggctgat gtcgccagcg
     2641 cctttggcct tggacaactc ctgggcttcc ttgaccatgc cgtaggcgat ggatcgttca
     2701 taaggaaaga cctgggtcac gatcacgccg cgcgcattct cgcccaggct tttgatgaag
     2761 ccggttgatg cattgttgga cagcgttacg atctgcgccg cggagccggc ggcgcggaag
     2821 gccttcatgc cgtcgaccac ggcctgaccg gaggccacca tgatgatggc ctgcgcgttg
     2881 gactggacaa ccttgggcgc gatcggcccg aagtcgggct tgctgcggtt gaatttttcc
     2941 agcaccgcag gctgcagctt ggcggcagtg aagcctttct gcgcgccggc cacgccgtcg
     3001 gcgccaaaac tgtcgtcggc ataaaccagg ccgatgcggg tgatgcccat tgagtgcagg
     3061 tggctgacgg ctttttcggc ctcacgctgg taggtggcgc gcacgttgaa cacatgtttc
     3121 ttgaccggct ggtgcaggac catggcgcca gtggaggggc cgatcagcgg cacaccgtat
     3181 ttttcgagga gaggaagaat ggctt
//
LOCUS       DAIDFG010000344         3199 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_344_length_3199_cov_19.349555, whole genome shotgun sequence.
ACCESSION   DAIDFG010000344 DAIDFG010000000
VERSION     DAIDFG010000344.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_344_length_3199_cov_19.349555"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          452 a         1069 c         1120 g          558 t
ORIGIN      
        1 cggtggtgac gactgttttc ccgaactggc ccgcatggcc atgggcaagg accgcatcat
       61 tgccgacggc gcgcagattg tcgcgcgcaa ccccgacatc atcatcggct cctggtgcgg
      121 caagaagttc cggcccgaaa aagtcgcggc ccggcctggt tgggaaaacg tcaacgccgt
      181 caaaaacggc cagctgttcg agatcaagtc ggccgacatc ctgcagcccg ggccggccgc
      241 gctgaccgac ggggtggcgc agttgcaccg catcgttctg gactggagcc tggcccatga
      301 ataagctgtc cctggctcgc agtgaactga tcctcggcgg ccagcgcagc ggcaagtcgc
      361 gccgcgccga aaggctggcg cagggctggc tcgcgcagtc acccgcgcat cgtgcggtgc
      421 tgatcgccac cggccagccc tgggacgacg agatgcgtca acgcatcgcg cgccaccagc
      481 aggacagggc tgagcgggtg cccggcatgt ccacggtcga ggaaccgctg cagcttgcgc
      541 aggcgatcac agtgcacggc aacgcccaga cgctggtggt ggtggactgc ctgacgctgt
      601 ggctcaccaa ctggctcatg cctgtcgaag tcttagataa aaatacgact ccagctcaat
      661 acccacgggc gcaggcagct atgtttttgg aagcaatcga aacttgcccc gggccgctgg
      721 tgctggtcgg caacgagatc ggattgggcg tgattccgct cgggcgcgag actcgcgcct
      781 ttgtcgatgc gctgggccgg ctcaaccagg aggtggcgca ggcctgtgag cgagtcaccc
      841 tgatggccgc cggtttgccg ctggttttga aaggcccgcc atgaacattt cttcttgctc
      901 ccccgccctc tgggagaggg ttggggtgag ggcggcgctg ctgcttttgc tgacttttct
      961 ggccgtggcg ccagcccacg ccctgcaact gatcgacgat cgcggtgtca gcgtgagttt
     1021 tgcgcaaagc ccgcagcgca ttgtcagcct gctgccttcg ctgactgaaa gtgtctgcgc
     1081 gatggagcaa tgccagcgcc tggtcggcgt ggaccggtat tcgaattacc ccgccagcgt
     1141 gcgcagcctg ccggtgctgg gcggcgggct ggaccccaac atcgaaggca tcgtggcgct
     1201 caagcccgac gtggtgctgc tggccacgtc gtcacgcgcc agccagcgcc tggagtcgct
     1261 gggcatcaag gtggttgcgc tggagcccaa gagccatgcc gacgtccgcc gcgtgctcgg
     1321 gaaaattggt gtcctgctcg acattcccga ggccgccggt gctggacggt tgtggcgcac
     1381 catcgacgcc agcgtcgagg cggcggcgca gtcgctgtcg ccccgggcca aacgcgcgcg
     1441 ggtgtatttt gaagtcaacc gcgggccgta tgccgccggc gagtcctcct tcatcggcga
     1501 aacgctcacc cgcctcggtg tcaaaaacgt ggtgccggcg tcgctgggcc ccttcccgcg
     1561 gctcaacccc gagttcgtcg tgcgtgccaa ccccgacgtc atcatggccg gcaaccgcag
     1621 catgcagggc atggcgtctt atcccggctg gggcagcatc aaggccgtga aggagcggcg
     1681 catctgctcg tttggtgtgg acgagtccga cgtggtcgtg cgtccgggcc cgcgcatggc
     1741 cgaggcggcc cgcctgatgg cccgctgcct cgaggacaag gcgccggacg caccatgagc
     1801 ccgggttttc ccaaccagca ccgccaggcc ctgtggctgg ccatcggcct gctgctggcc
     1861 agcgcggccc tgctgctgtt gggtgtcagt gtgggcagca ccggcgtcga gagtgtgcgc
     1921 agcatgtggc atgacgcaca ggccctgcag atcgtgcgcg agatccggct gccgcgaacg
     1981 gtgggggcct ggctggccgg ggccttgctg ggcctggccg gcgcggtggc gcaaggcctg
     2041 tttcgcaacc cgctggccga cccctacctg ctgggcagcg cctcgggtgc gtcgctgggc
     2101 gtggccagtg cgatggcgct gttcggcgtg tcgcccctgg ccatgcactg gctcgcgcgc
     2161 atcgggctca ccggcgcggc ctttgtcggc gccgtgctgg ccgtgctgct gaccctgctg
     2221 ctggccaagg gtgtgcagca cacgctgcgc ctgctgctgg ccggcgtgat tgtcggcgtg
     2281 gtgctgggcg ccttcacctc gctgattttg ctgctcacgc ccgagatcat gcaggccatg
     2341 cagtccttca tgctgggcag caccggtttt gtcggctgga ccgcctgcgc cgtgatggcg
     2401 gcggtgctcg ccttcagcgt gctggccgcc tggatgatga gccatgtgct cgatggcctg
     2461 gcgctgggcg aagccaccgc gcaaagcctg ggcctgcgcc tgccgcagat gcgggtggcg
     2521 ctggtggcgg tgctggcgct cgccaccggc accgccgtgg cacagaccgg gctgatcgcc
     2581 tttgtcggcc tggccgcacc gcacctggtg cgctccgtcg taaagaccac ccacggccgg
     2641 ctgattgtgc tggctgcctt gatgggcgcg gtgctgctga cggccgccga catcctggcg
     2701 cgctggctgg tggcgccgca ggaactgccg gtgggcgtgc tgacggcggt gctgggcggc
     2761 ggctacctgc tctggctgat gcaccggcac accggacggg gggcggggct gtgatgccaa
     2821 acagctcttc agttgctatg gattcagtag ctataaacgc ccgcgccatc agggccagcc
     2881 tcggacatgt cgaggtcctg cacgacatct cgttggcgct gccggccgcg cgctggacca
     2941 gcatcgtcgg ccccaacggc gccggcaagt ccaccctgct caaggtgctg gccggcctgc
     3001 tgccgcacca tggcgaagtc gccttgctgg gccagccgct ggcccgcctg cggggtcgcc
     3061 agcgtgcgca gcagctgtcc tggctgggcc agaacgaaag ttcggccgac gacctgacgg
     3121 cttacgacgt ggccatgctg ggccgcctgc cgcaccaggc ctggctggcg ccgcccagcg
     3181 ccgccgacca tgccgccgt
//
LOCUS       DAIDFG010000345         3179 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_345_length_3179_cov_18.364277, whole genome shotgun sequence.
ACCESSION   DAIDFG010000345 DAIDFG010000000
VERSION     DAIDFG010000345.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_345_length_3179_cov_18.364277"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          544 a         1053 c         1030 g          552 t
ORIGIN      
        1 caatcgcctg gtcggtcagc gacagcgcgt cgcgcatgga gccgcgtgcg gcgcgcgcca
       61 gcaggcgcag ggcctgcggc tccgactcga tgttttcggc ccgcagcacc tgcgtcaggt
      121 gctcgaggat ggtttccggc gccatcggcc gcaggttgaa ctgcaggcag cgcgacagca
      181 cggtggccgg cactttctgc gggtcggtcg tggccagcac aaacttgagg tactcgggcg
      241 gttcttccag cgtcttgagc atggcgttga acgccgtgtt cgtgagcatg tgaacctcgt
      301 cgatcatgaa gaccttgaag cgtcccacca ccggcttgta gaccgcctgc tcgagcaact
      361 gcgcaatctc gtcgacgccg cggttggatg cggcgtcgag ttcggtgtaa tcgacaaacc
      421 ggccgctgtc gatgtcggtg caggcctggc agacgccaca cggcgtggcg gtgatgccgc
      481 cctgcccgtc cacacccagg caattgagcg atttggcgag gatgcgcgag atcgtggtct
      541 tgcccacgcc gcgcgtgccg gtgaacaggt aggcatggtg cagccgctgc gtgcccagtg
      601 cgttgctgag cgcctgcacc acatgcgact gcccgaccat ttcagaaaaa ttccgggggc
      661 ggtatttgcg ggcaaggacg aggtaggaca ttccctgatt ctacggttgc gccggagccc
      721 ctcagtgcgg gtaatcggcc gaacccggcg caaaactgat gcgccgcgtc tccaccaggc
      781 gcccggagcc gccgggggct gtcaatatcc ggccttccac cagttcatac cccagcaggg
      841 caaaaacccg gttgcgcaga aaaatcgggc gggcattgcc ataccagtcg gtgcaggagg
      901 cgcggcagcc gtcgtccctg ccgacacccg gccgcgacgc gaggctgccg ataccggaca
      961 acttcaggcg gttgttgcgc aggtacagca tgccggcggg ttcgtcccac agctggcggt
     1021 agccgccctg ccccgcaccg cgcaccggca ggcccaccac cccggaggtg gcgtcatcgg
     1081 gcttgtagaa aaaaccgtgg ctgcgcgtct cgccctgcgc ggcctgcgcc tgcacgtaac
     1141 gcccggccac gcgcgcccgg ggtgccagtt gcacactgga aaaatgcagg tcctgcccgt
     1201 ccgtgcccac ggcaatcgca tcggcgccca gcgcctcgat gcggtccacg ccgtgcggca
     1261 gcgccagggt ttcgcctctg gcggggtcag ccagatgcac cacctgcaac tggcccagcg
     1321 tgcccggcgc gggccggcgc cagccgctgc ccgcaccgta gagcaggcgc tggccgacaa
     1381 aacggttctg caggctgccg tccccggcgg ccggcaaacg tgtataggcc tgctcaccgg
     1441 cacgggtctg gccatcaccg aaatcacgca ccggcacacg cagcagggac aggttgccgc
     1501 cgctgccttc gccgcgccac atgccgtccc cgttgccgtt ggcgcgcagc agcacattca
     1561 ggtagccgtc gtcgccctcc agaaacgaca tctggtcgat cgggctgccc tgcgttttga
     1621 gcatgctggg cgccgcgccg tcgagcggca ggcgaaacac cgccgaatgc gatgcgacac
     1681 gcccgctggc ggcatcgcgc tggttgcgcg tggtccacac atacaccgcc cggctcgaga
     1741 cataaaacac ccggccggcc ggccccagca cggcgctgga gcggcagtcc agttcaggca
     1801 gggccaggtc gcagcgcacg atggtgtgca gggtgatgcc ttcctgcggg ttcagcggtt
     1861 cgtccgtgcg gtaaatacgt gttgcgtcgg cgatgcgttt gaagtccgcg ggtgtcgcgc
     1921 ccggctgcca gcgccgcatc gcgggcaggc gtgccgccgg atcggcatgc cagaggttca
     1981 gcggaatggg ggtgtacagg atgagctcgc tgccaatcag gcggctggcg tagttgcgcg
     2041 aggagtagta gtcgctggag cgcacatggt atgtggcgcg gtagctgagc cggcccgcgt
     2101 catcgatgtt gaacaggcca agctccgtcc cgccgcgcga atagctgtag ccgatcacgg
     2161 ccaccgtgtt gccggacacc atcatctcgt cataccaggc gcccgatggg ttgacatccg
     2221 gcgcgtaggc gttgatggac gacaccggca gcagttggtg gcggtccaca tcgaccgtga
     2281 acaagcgtcc ccggcgcagg atgaccaggt gcttgccatg caccttgaca atgccgccct
     2341 catccacgcc ggccgtctgg ttgttggtga tggactcggc ggcgccggcc gcggcaggcg
     2401 ccggagcagc catggccatg gcggtcgggg cgggagggtt cggccgggag gcccaggtct
     2461 tgcccgggtt ctcggcctcc cattttttcc ggacttcttc ctgccgcagc cggcgttcct
     2521 cttcctgcct gcggcgttct tcctcgcggc gctggcgctc caccacaaag ggctcgaacc
     2581 aggcctgcaa ggcggcctcg tcgacaaagg ctgtcaggtt tctggccggt gtgaaggcct
     2641 caccctgtgc ctgtgcctgc gcctgcgcga caagccctgc accacacatc gccacccccg
     2701 cgagggcctt caacaaccag gacatgtttt tttccttgaa aggccagact atttttaagc
     2761 tattcaatga tattcgggcc gggggcgccg cgcctcgcat aaaatagtga gtgacgggcc
     2821 ttcccgcatg gtgaagcggc caaccgggtc agatggggaa ccaagcagcc ctaactgtcg
     2881 agccagtgcc ggggtcgagg tccgtcacct tatttcaaag aacagggcat ccatggatgc
     2941 cctgtttttt ttcacctgca cgctgatgca gtgaaaaaat tcacatccag cccccgccac
     3001 gccaaattcc tacaaacgga tgcggcgcct gcctgccgtt tcgggctttc tccatcgcaa
     3061 acttttccct agaatttttt gcatggatac atttgaaaaa ccaacccacg aggaactggc
     3121 ccgctacctg aaggcactgg gccggctgat caaggcggag tcacaacggc tggaggcaa
//
LOCUS       DAIDFG010000346         3178 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_346_length_3178_cov_17.270573, whole genome shotgun sequence.
ACCESSION   DAIDFG010000346 DAIDFG010000000
VERSION     DAIDFG010000346.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_346_length_3178_cov_17.270573"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          549 a         1009 c          991 g          629 t
ORIGIN      
        1 acgttggggt tgcgcgccat cagcgccttg agcggcacgg cggccaggtc gtcggtgcgc
       61 acgctgctga gcgcgccgcc atatcggccg aaaggggtac ggatggcgtc gcagataaaa
      121 gcctgattca tgtgtgtcct ttagtgaaag tttccctctc cctttgggag agggtgtggg
      181 tgagggctct tcgtgtgccg gcttgccctc acccctgccc tctgccggag ggagagggag
      241 caataccaat cggcaggccg accagcttct ccagctcgtc gtgcgccagc ccctcgactt
      301 tatccaccag ccggaggcct tgcggcgtgc attccagcgt ggccaggtcg gagtagatgc
      361 gtttgacgca ggctatgccg gtcagcgggt agctgcaggt cttgaccact ttgctctcgc
      421 cttttttggt cagcaggtcc atcatgaccc aggtctgcct ggcaccgatg gcgaggtcca
      481 tggcaccgcc cacggccgga atggcgccgg gctcgccggt gctccagttg gccaggtcgc
      541 cggtgcttga gacctgaaag gcgccgagca cacagatgtc caggtggccg ccgcgcatca
      601 tcgcgaagct gtcggcatgg tggaaatagg cgccgccctt gagcagggtc accggctgct
      661 tgccggcatt gatcaggtca aagtcttcgt cgccctcggc tggcgccggg cccatgccca
      721 ggatgccgtt ttcgctctgc aggatgacct cgattccttc gggcaggtgg ttcgcaacct
      781 gcgtcggcat gcctatgccc aggttcacgt aggcgccgtc atggatgtca cgcgccacac
      841 gggcggcgag ctggtctttg ctgcgtttct ggtaagtcat gtcaggctgc ctgtttgaag
      901 ccgccggcct gcgtggcgac acgctcaatt ttcacgatcc ggtgcacaaa gatgccgggg
      961 gtcacgatgt gttccgggtc cagcgcgccg agctccacga tctcgtgcac ggtcgccacc
     1021 gtttgtctgg ccgctgtcgc catgaccggg ccaaagttgc gcgccgcctt gcggtacacc
     1081 aggttgcccc agcggtcgcc gcactcggcc ttgatcagcg ccacgtcgcc gtagatcggc
     1141 atttccagca cgtagtgccg gccattgatt tcgcgggttt ccttgggcga accatcggcc
     1201 tgtttggcca gctcggtgcc gaagccggtg ggggaaaaga acgcgccgat gccggccccc
     1261 gccgcgcgca ggcgctcggc caggttgccc tgcggcacca gttccagctc gagcttgccg
     1321 gtgcggtaca gctcgtcaaa aacataactg tcggcctggc gcggaaaact gcagatgatc
     1381 ttgcgcacgc ggccggtctt gagcagcgcc gcgagaccaa tgtcggcatt gccggcgttg
     1441 ttgttgacca cggtcaggtc cttcgcaccc tgcgcaatga gcccgtctat cagttcgttc
     1501 gggatgccgg ccgtgccgaa gccgccgatc agcacggttg agccgtccct gacaccggcc
     1561 agcgcctcgg ccaccgaccg ggctgtcttg ttgatcatga aactgtctcc gtcggaaaaa
     1621 tcattcgcct gaaatttgtt cttatgtaga acaaatgttc gtataatgaa ttttaggaga
     1681 tttccgcacc catggcaatt gctgcatccc cttcgggccc tgatggccgt cccggccccg
     1741 cgccgggcga cagttatgtc cagtcctttg cacgcgggct ggaggtcatc cgctccttca
     1801 gcgcgcaatc gccccgccag acgctgagcg aggtggcggc gcgcagcggc ttgacccgcg
     1861 ccggtgcgcg gcgcattttg ctgaccctgc agacgctcgg ttatgtcgtc agcgacggca
     1921 agttgttttc cctgaccccg cgcatcctgg acctgggctt tgcctacctg tcgtccatgc
     1981 cgatctggaa cctggccgaa ccggtgatgg agggtcttgc cggggaagtc ggggagtcgt
     2041 gttctgccgc ggtgctggaa ggcacggaca ttgtgtatgt gatgcgggtg gccacccaca
     2101 agatcatgag catcacgctg ggggtcggtt cgcgcctgcc ggcctactgc acgtccatgg
     2161 gccgcgtgtt gctggccggc ttgccagagg gcgaagcgct ggcttgcctg aaagcctcgg
     2221 aactggcgcc gctcacccgc cacacgctga cggacctgga cgccttgcgc cagaaaattg
     2281 cgcagacgcg caagcagggc tggtgcctgg tgaaccagga actcgaagaa ggcctggtgt
     2341 ccatggccgc gccgattcac gaccggctag gcaagacggt ggcggccctg aacatcagtg
     2401 gccaggccaa ccggaccagt gccagggcca tgcaggacag catgctgccg gccctgctgg
     2461 ccgccgcgca aacgatttca cgcctgctca gcgttcaacg cacctgatcg ggccatgacc
     2521 cgacgggccg ttcaggactt gcgggaaccc atgaccagca ccacggcgcc caggacaatc
     2581 gcgccaatgc tgagccactg gggcacattg accgactctt tttccttgac gaccagttcg
     2641 agcgggccga tgttggcttt ggtggtgtcc ttggtgaagc tgaaaccgcc ggtgaaaaaa
     2701 ccggccaggc cgagcacgat caggacaatg cctgtgattc tgactgcatt catgaaattc
     2761 tcctcgccgg atgatgggta tgccggcgcg cgcttgccgg gatcacgctg ccgtgcaggt
     2821 aatctagccg ataagaccct gcagggcgcg taggacattg ccgacggacc ttttttggcg
     2881 tcccggaaac accacagcag caacaagcac agaccccatg ctacgcttga cccatgttca
     2941 cgccttcaca agccgacgtc cgtcgttttt tttgttccgt ttacgccaaa gcccgctccg
     3001 ggcaagcact ggaagctatt gaaatcatag caagccagtg gatcgacgag caccccgagt
     3061 accacgccga ctttgccgat gccgatgcag cgctgcagaa gatgtacgac gctgaagacg
     3121 gcaagaccaa ccccttcctt cacctgtcca tgcacctgtc gatcagcgag cagtgttc
//
LOCUS       DAIDFG010000347         3168 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_347_length_3168_cov_14.220366, whole genome shotgun sequence.
ACCESSION   DAIDFG010000347 DAIDFG010000000
VERSION     DAIDFG010000347.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_347_length_3168_cov_14.220366"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          541 a         1107 c          962 g          558 t
ORIGIN      
        1 ggcgggtctg atgcggcagg tggggctgga ccccgactac ggccagcgct acccccacca
       61 gttctcgggc ggccagcgcc agcgcatcgg cattgcgcgc gcgctggcgc tcaagccgca
      121 gctcatcgtg tgcgacgaag ccgtggccgc gctggacgtt tccatccagg cgcaggtgct
      181 caatctgttt ctggagttgc gccgcagttt tgatctgacc tatctgttca tcagccacaa
      241 cctgggcgtg gtgggccatg tgtcggaccg cgtggtcatc atgtaccttg gccgcgtggt
      301 cgagatcgcg cccaccgagg tgattttcag ccgccccaac cacccctata cgcaagccct
      361 gctgtcggag ctgcctacgc tggacaccca gcggcgcgac taccagccca tcaagggcga
      421 gctgccttca ccgttgcggc cgcccacggg ctgcgccttt cacccgcgct gtccgcacgc
      481 catgccgcgc tgccagatcg aactgccccc gctgcgcacc ctggccaccg gccacctcag
      541 cgcctgccat ctcaacgacg cggcgcctgc cgtgtccagc ctgacctcat gacccatcct
      601 gttctgcccc gcgtcctcac cccgtttgac ccctacactc tgcgcctgcc cgaagccggc
      661 gtcgtgccac tgatctgcga ttcaccgcac agcggcacgc tgtaccccga cgacttcggc
      721 tacgccttgc cgtttgaaat actgcgcaag ggtgaagaca ccgatgtgca tgtgttgtgg
      781 gaggcgctgc cgtcggtcgg tgctacgctg ctggccgcga actttccgcg tgcctatgtg
      841 gaccccaacc gcgacgtcga agacctggac gccaccctgc tcgacgcgcc ctggcccacc
      901 cccctggccc ccagcgaaaa aacccgtctg ggcattggcc tgatctggcg cgacgcgagc
      961 aagggcgggc aagaccccat ctacgcgcgc aagctcagcg tcgccgaggt gcaaaaccgc
     1021 atcagccgct accacgcgcc ctaccacgcg gccatgcagg cgcagattga agcggcctat
     1081 gcgcgctttg gcgctgtctg gcacctcaac ctgcattcaa tgcctgccaa ctcgtacgag
     1141 agcctgcaga tccagagcga ccacccgctg gccgacgtgg tgctgggcga ccgtgacggc
     1201 accaccgccg cccccgaatt caccgccatg gtggccagcg cattccgcca gcgcggtttc
     1261 agcgtcgcca tcaacgaccc gttcaagggc gtggcgctga tcgcccgact gggccggccg
     1321 gccgagcgcc gccacagcct gcaggtcgaa ctgcaccgcg gcctctacat ggacgaaaag
     1381 acccgagccc gcagcgtgga ctttgtcacc ctgcaaagcg cactggcggg catttctgct
     1441 gaaattgccg cttacgtcca agcccagatc ccttcctgac cagagaaccc cgccatgagc
     1501 ctgatcgccg aaatcgaagc cacccacgac gaattcaccg cgctgcgccg cgacatccac
     1561 gcgcatcccg agctggcctt ccaggaaaac cgcacgtccg acgtcgtggc tgagcggctc
     1621 gcgtcctggg gcatcgaggt gcatcgcggg ctgggccaga ccggcgtggt gggggtgctg
     1681 aaaggctccc aaaccaaagc cggcagcaag ccgcgatcca ttggcctgcg cgccgacatg
     1741 gacgcgctgc ccatgcccga gcacaaccgt tttgcccatg cttccaaaaa ccccggccgc
     1801 atgcacggct gcggtcacga cggccacacc gccatgctgc tgggcgccgc caagtacctg
     1861 gccgcgcacc gcgactttga cggcatcgtc aacttcatct ttcagccggc agaggagggt
     1921 ggcaatgccg gcgccaaggc catgatggcc gatggcctgt ttgacaagtt tccttgcgac
     1981 gagatttacg gtatccacaa catgccgggc tttgacgtcg gccatttcgg tttccgcaag
     2041 ggaccgacca tggcgtcgag caaccgcttt gacatttttg tgcgcggcac cggcggccac
     2101 gctgcgcagc cgcacaagtc ggtggacacc atcgtcatcg cggctgaaat ggtgggcgtg
     2161 ctgcagacgc tcatctcgcg ccacaagaac ccgattgaca ttgcggtgct cagcatcacg
     2221 cagattcacg cgggcgatgc gtacaacgtg ctgcccggcg agaccgtgat ccgcggcacc
     2281 gtgcggacct tcagtaccca ggtgctggac gacatcgagt ccaacatgcg ccgtgtggcc
     2341 gagatgctgc cccaggtgca tggcggcagc ggcgagctca agttccaccg cgcttacccg
     2401 ccgctcgtca actgggacga gcagaccgac tttgccatga aggtggcgca agacgcgttc
     2461 ggcgctgacc atgttgacgg ccacacgccc cagcacggcg gcgccgagga cttctctttc
     2521 tatctggaga aagtgccggg ctgctacctg ttcctgggca atggcgaagg cgcgcaccgc
     2581 gacgaaaagt acatcggcat gggcccctgc gaactgcaca accccaacta tgacttcaac
     2641 gacgcgttgc tgccgatcgg gtcgagttat tgggtgaagc tggtgggcgc gtttttcgcc
     2701 cgataggacg tttccgggct aggcgctggt cttcatcagg gccggcagga cacgcggcat
     2761 gacgataggc cactagggcc tggccagctt ctcgccatgg tagctggtga cgcgctcgac
     2821 ttcgttcttc gaacccagga tcacgctgac acgttcgtgc agcttgccgg gctggatgtc
     2881 caggatgcgc tggtggccgt tggtggcggc gccgccggcc tgctcgatca gccagctcat
     2941 ggggttggct tcgtacatca ggcgcagctt gccggccttt tcgggctcgc gtttgtccca
     3001 ggggtacatg aagatgccgc cgcggcacag gatgcggtgc acgtcggcca ccatggcggc
     3061 cacccagcgc atgttgaagt ccttgccgcg cggaccgtcc ttgccggcca gcagttcgtc
     3121 cacgtagcgt ttgaccggct cgtcccagtg ccgcatgttg ctcatgtt
//
LOCUS       DAIDFG010000348         3130 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_348_length_3130_cov_17.733984, whole genome shotgun sequence.
ACCESSION   DAIDFG010000348 DAIDFG010000000
VERSION     DAIDFG010000348.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_348_length_3130_cov_17.733984"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          593 a         1141 c          992 g          404 t
ORIGIN      
        1 actcctgcac cagcgccgca aacgaactca gttcttccgg ccgtttgtcc acacacatca
       61 gcggcaccag cgcgccgccc tggccggcct caaaacgctc gcgctgcacc ggcgtgctgt
      121 cctcgggcag ctccacgccg gtgaacacaa acagcaggcg ctgcggctcg ggctgggccc
      181 gggctgcctg cagcaggtca tcaaaactca gaatgtccat gaatccatcc agtcacaggg
      241 aataaaaaga atccgcgggt tcgcgccccg gcgcgtgccc gtcggccgaa aggctgcttg
      301 taaaatccgg cggcgcaggg cgcaggcgcg cgggcttgcg cgactgtgcc gtgccgatgc
      361 tgacaaaaca cagcgcctgt tcgccggccg ccaggccaaa cagggtgcgc aggccggcag
      421 acttcagcgc cttgccgctg gtcaatgccg aaccataccc caatgcagta gccatgagca
      481 gcatgttctg gatcgcgcag ccggcggaca cggtgcgttc ggccagatca atctccgcat
      541 cgccgcgctg gccatcgacg atcaccagca tcagcagggg ggcgcggtgc gctttttcac
      601 gcgcctgcgc cagctgttcc gcagtagcct gggcgtcgcg cgccatcagc gccgcgccga
      661 acacctcggc cagccgcgcg cgcgccgcct gcggcacgag caccaggcgc cagggttgca
      721 gctgcccgtg gtcgggcgcc gtggccgccg cgttcaggat catgccgagc tgcttcgcgt
      781 cgggcccggg cgccagcagg cgcttgggca aaatggtctg gcgtgactgc atcagcgtcg
      841 ccgccaggat ggcgggatcg tggtcaggcg cggtagatag tggcaacggg gccgggacgg
      901 caagcaggtt catcatgaat actcccctca gccaggccgc gcgtcagcac gcaggcggcc
      961 ataccagttg cccagccgca caccccacac ccccatgacg ccggcccaca acagcgcggc
     1021 cgccagcagc agccagcccg cgagggccgt ttgcgcggca gccgcaatgc gcagcagcgt
     1081 cgcggcctgc agcagccaga acagtgtcca gatcaggttg tcggccacca gcgcacggcc
     1141 gctgtggcca caggacaccc gcgtgaccat ggccagcatc agcgacgcca ggcaacccat
     1201 ggtcagcgca tgcagcgacc cgagggccaa caccggcgcg tcctgcgcca ggcccagcca
     1261 ctgcgagagc cctccgagca ccagcgccag gccgagccag aaaaaaccga tgtgcagcat
     1321 ggcgagcaaa cggttcttga ggctttgcac cagcccccag gcaacagcga gccagagcag
     1381 cacgccgccc gcggccagct cgaaggcccc gcgcgcccca ctccagaccg tgcccgccat
     1441 gccttgcaac gggccgtcca gctcgaccca cacggcgagg acctcggccg ccgcgacggc
     1501 cagcatcagc cacaacaccc agaacggacg ccaggcgtcg atgcgcggca gggcactgga
     1561 agtgaaaaac ggaatcatgc gatgcgccac cgaaacgtag accaccacca caaaccccca
     1621 caaaccggtg agcatgcagg cgcgcgccac atcgggcccg cccagcagca ggctgagcgc
     1681 caaaccggcc agactcaggc agcccacgat acaggccgcg cccaccgttc gcgcatgcac
     1741 ctggtcgtcg gcccggctgc gacgaaccag cccccagaac agcgccgtca cccaggacag
     1801 tcccgcacag gccagcacca gcccggccaa tgccgtcaaa ccatgcagat gcgcgccaac
     1861 cagacacagc agccagccgg tcacctgcag cagcagcggc acccgcaact ggcgggacac
     1921 caagggctgc acgcccagcc atttgggccc ggccgtgaac aggaaccccg cgaagaacag
     1981 cggaataaaa ccaaaggtca tgaccgcgcc atgggccagc gagggcgaca gcgcataggg
     2041 caggctcagc acgggactga ggcggtccag ctgcaccagc gcccaccagc tgcctgcggc
     2101 caccagcacc accatggcca ggaaaaaccc cagccggtgc ggcgccagca gcagatggct
     2161 ggcgcgccag cgggggtcca ccctggcccg ctcgctggcc gtgggtttgc ccggcctcac
     2221 ccgcagggcg atgaccgggc gctcactcat ccgcaaacgc ccaagtggcc gcactgggtg
     2281 cagtagtcgc aaccatcctt gcggatcacc gcgtgggcgc cacattcgct gcacttcttg
     2341 cccgccatca ccggcggcgc gagcgccatg tcgacctggg gctccaccgc gggtgcgacg
     2401 ctggcggcgc cctgctgggc gcggcgcgca atgatgttct gcaccgcata ggcaatggcc
     2461 gcaacttccg agtcgtgcca cagcggcacg cgtgtgccgt cggttttctc ataggtgccg
     2521 agccgcaccg ggccacggtc ccaggcgacc ttgcgcatgt cgctgagcgc gcgctcgagg
     2581 aagccgccgc gcgcagccag cgacagcatg cgcatgctgg aggtgatcca ctgctgcgac
     2641 tcgccgcttt ggcccaccgg catgaaaaat tcaatggcac gttcgaccgt gccctcgccg
     2701 ctggctgagg gcaccggcag gaaagacacc acgatgtaca gcgtcttgtg cccttcctgc
     2761 gtccagtagt cgaccttttc ggccacggcc gacaaggcgc ccttgggacg gctctcgatc
     2821 accgtgcgca tcggatccat gggcaccggc gcggccggcg cggcggcggg agcatgggtt
     2881 tccagcaccg agcccagaat cgcattgggc cggtaggtgg ccaggccctt gagtttggcg
     2941 cgccaggcct gctggtacag gctcttgaaa tcctcgtagg cgtagtcggc cggcacgttg
     3001 acggtcttgg agatcgccgt gtccacaaag ggctgcaccg cttccatcat gccaatgtgg
     3061 ctgtctgccg acatctcgag cgccgaaaca aagtaggcgg gcagtttgtc cacgttgccg
     3121 ccgagttcgc
//
LOCUS       DAIDFG010000349         3105 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_349_length_3105_cov_17.201050, whole genome shotgun sequence.
ACCESSION   DAIDFG010000349 DAIDFG010000000
VERSION     DAIDFG010000349.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_349_length_3105_cov_17.201050"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          588 a         1069 c          934 g          514 t
ORIGIN      
        1 cacgcttgtc gctgcgcgta ctgcgctgcc ccccgagggg ggcgctgcgc ctgcgggccg
       61 gcgaagccgg acccgcggcc ccggctggta aagacagggc tgcggcttgt gtcttgtgtc
      121 tcctctgaat gattgattga tcggaaccga cgatgactgc gcagaccatg agcacgatgg
      181 aacgtatcct gcgtttgatg gccgataaaa aggcctccga cgtctatctg tcggcgcatt
      241 cgccggccct catcaagatc aacggcgagg ccattccgat caacagccag atcctgccgc
      301 cggacgcgcc gcgcaacctg ctggcggagg tgttgcctcc cgagcgcatc gaggagctcg
      361 aagaactcgg tgagctcaac atgggctttc cgctggccgg cgtcggcaat ttccgcatca
      421 gcggtttcag gcagcgcagc acctatgcgg ccgtgatccg ctacgtgccc ggtgagattc
      481 cgccgctcga gagcctgaac gtgccgccca tcctggccga cctgatcatg gaaaaacgcg
      541 gcctgctgct gatggtgggc tccaccggcg ccggaaaaag caccaccctg gcgtccatga
      601 tggaccaccg caacgcaacc atgccgggcc acatcctgac gatcgaagac ccggtcgagt
      661 ttttgttcac caacaagaag tcggtggtca accagcgcga ggtcggcagc gacacgcagt
      721 cgctgcaggt ggcgctgaaa aacgcgttgc gccaggcgcc cgacgtgatc ctgatcggcg
      781 agatccgcga ccgcgaaacc atgtcagccg cgattgccta tgcgcagtcg ggtcacctgt
      841 gcctggccac catgcatgcc aacaacagct accaggcgct caaccgcatc ctgagttttt
      901 acccggtcga ggtgcgcaac accatgctcg gcgacctcgg cgcggccatg aaagccatcg
      961 tctcgcagcg cctgctgcgc acgcaggcgg gcggccgcgc gccggcagtg gaggtcatgc
     1021 tcaacaccaa gctgatctcc gagctgatcg aaaaaggcga cttctccggc gtcaaggaag
     1081 cgatggaaaa gtccatggcc gagggctcgc agaccttcga gcaggacatc gccaagctga
     1141 tcgttgacgg caccgtcacg cgcaaggaag gcctgctgca tgccgactcg ccgaccaacc
     1201 tgatgtggcg cctggaaaac gacttcgcca tggccgccaa ggcggcacaa gagcccgaag
     1261 aagatgagga cgacgaagcc tccttcacag agatcacgct cgacgttcgt cacgacacca
     1321 agaccggcaa caccggttat ggcaacctgt aagcgcagcc ccggggtcct cctttttcct
     1381 tcgtttcctt tttcatgtct tccacactcc gacttgctga acaactgatt tcccgaccct
     1441 cggtgacacc cgacgacgcg ggttgccagg ccctcctggc cgcgcgtctg caggcgctgg
     1501 gttttgaatg tgaaaccatc gtcagcgggc cagagcactt ccgcgtcacc aacctgtggg
     1561 caacattcaa gggttttggc cctgtagccc agacgggacg ggcgcaggca gctcctgatt
     1621 cgatagcacc cagaaccctg gtctttgccg gccacaccga tgtggtgccg accggcccgc
     1681 tggagcagtg gaccagcccg cccttcaccc ccagccaccg cgacggtgtg ctctacgggc
     1741 gcggcgccgc ggacatgaaa acctcgatcg cggccatggt cgtggccgtc gaggaatttc
     1801 tcgcggccac cccgcagccg aatctctcca tcgccttcct gatcaccagc gacgaggaag
     1861 gcccgtcggt ggacggcacg gccgtggtgg tgcagcagct caaggcgcgc ggcgaggtgc
     1921 tggactactg catcgtcggc gagccgacct cggtggacca gctcggcgac atgatcaaga
     1981 acggccgccg cggcacgctg agcggcaagc tcacggtcaa gggcgtgcag ggccacatcg
     2041 cctacccgca cctggccaaa aacccgattc acgactttgc ccccgcgctg gccgaactgg
     2101 tcgccaccga atgggatgcc ggcaacacct ttttcccggc caccagctgg caggtgtcga
     2161 atgtgcacgg cggcaccggt gcgtccaaca tcatcccggg cgagctggtg gtggacttca
     2221 acttccgctt ttcgaccgaa tcgaccgccg acagcctgaa agcgcggctc gaggccatcc
     2281 tgcaaaaaca ccagctcgac tacacgctga cctggaccct gggcggccag ccctttctga
     2341 ccacgcccgg cgagctggtg caggccgtca gcgagtccat acgcgccgag accggtctgc
     2401 agaccgaact ctcgaccacc ggcggcacca gcgacgggcg ttttatcgcc agcatctgcc
     2461 cgcaactgat cgagttcggc ccgatcaacg cgtccatcca caagatcgac gaacacgtgc
     2521 tggtcagcac gctggacccg ctcaaaaaca tctacagggg cgtgctggag cgcctcgccg
     2581 gccagtgagc gcagcgcctg ccccgacgac cgtcatcgcc ttggtcgagt actgggccga
     2641 ccgcctcgag gccgccggcc tcaccttggc cgacggtttc ggccagggca cgctgaccgc
     2701 tttcgacgaa gccgcctggc tggtgctgtg gtcgctgggc ctgccgctgg acgagctcga
     2761 cgaagaggaa aatacgcctg tatcccccgc cgatgctgca aaggttgcta tccttctgga
     2821 agcacgcatc aacacccgca aacccgccgc ctacctgacg cgggaggcct ggctgcaggg
     2881 cgtgcccttt tatgtcgatg aacgcgtgat cattccgcgc tcgctgattg ccgaactgct
     2941 ggtcgatgaa agcatcgacc cctggctcag cgcgtccacc caccgtgtgc tggacctgtg
     3001 caccggcaac ggcagcctgg ccatcctcgc cgccatggcg taccccgacg tggccgtcga
     3061 tgccgccgac atcagtaccg acgcgctggc cgtggcccgc atcaa
//
LOCUS       DAIDFG010000350         3099 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_350_length_3099_cov_17.964849, whole genome shotgun sequence.
ACCESSION   DAIDFG010000350 DAIDFG010000000
VERSION     DAIDFG010000350.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_350_length_3099_cov_17.964849"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          599 a         1094 c          943 g          463 t
ORIGIN      
        1 gctcagccgg tcctggaaac gcaccttgta gccgaccaca tcgcccagcg gtgtcttgag
       61 ttcttcggca atccgcttgg ccacgctgga ggcggcaatg cggcgcggct gggtgtggcc
      121 gatcagccgg ccctggcccg ccgggtagtt gagcttgccg cggcccatgg ccagcgcaat
      181 cttgggcaac tgcgtggttt tgcccgagcc ggtttcgccg caaacgatga cgacctggtg
      241 ggcctggatg gcggcggtga tttcctcgcg tttgcccgag acgggcaagg actcggggaa
      301 atcgatctgc aagggcacgg acaaggggga cgaggacaag ggatgggggt tcaaaaccac
      361 tgattatcgg tgctggcggg cgcccgcccg gcgacagggc ttcaggcggc ttgcagcacc
      421 ggcagctcga ccgcttcgcc gctggcctgg tccagccagg cgacccggct ggcatacgac
      481 atggcggtgc gcgacatcgc cttgcaggcg gccagcacgc tcgaagccct ggccgccggg
      541 tcggacacca gcaacacgcc gacaccgatt tcagcctgcc agcgcgtctg ctcgctttcg
      601 cgccgcgccg catcgaggct ggtgttgaga accaccgatt tggacaggcg tttttccacc
      661 aggtgcgcca gccggatcag ctgaccactg tcgttgcagt cacgcagaag cagcacaaag
      721 ccgtcacccg aaaaccgtcc cgtctcgacg taagccggca ccgagcgccg caagcgcccg
      781 gcacagacaa acagcgcgtg gttgaccgcg gccatgccat gcaggcgctc cagcgcgtag
      841 aggttgccta tgctcaccac catcacgccc acttgcggcc cggaccggtg gggcgccggc
      901 ggcgcgccgt ccgctgcggc atctgaccca gccggatcgt cccggcgacg ccggaagatg
      961 tccccaacca tctgcccggt ttcggtgtgt gaacgcatgc gggtcaccgg gtcgtaactg
     1021 gggcccatcg ccatgacctg gtgcagctcg atcaggtagg aataacgcgt ccagagcgcc
     1081 acggccatgg tcacgaggta ggccgttccg gcaagcgcgc tgaccgcatg caccggccag
     1141 ggctcctgcc cgcgcaccag cgcgatccag cccaggccca gcatggccac cagcatgaaa
     1201 aacaccccga ataccgctgt ccaggccagc cggtccccgc ggtacacgct gcgcatcgac
     1261 acggcgagtg ccacggcacc cagggtccag gcgaacccga gacccagcac gagcgagggc
     1321 gccggcggca gcagccagcc cgcggccatc acgcccagca ccagcaccgt cagcgccagc
     1381 cgggtgtgca gccgcgcctt cctgctggcc agcaggccca ggatggaaaa cagcaggtaa
     1441 gccagaaagc ccgagaccag catcgcaatg tgcgccagaa cccgcgccag cgtgtcttca
     1501 tcgtcaatcg gcaggccacc cagaaaggcg accacaaaaa aacccgaggc cgtggccgac
     1561 aggccggcgt tgagcgagat gcggtgcagc gaacggacaa acgcgagtgc cgcgcccgca
     1621 agcatcacgg ccgccgtgcc gaaatagaca ccccagaaac caaacgcaag tttgtccatg
     1681 aatggccgca ttatccggcg aaacggccgc cgccaacagg caccaacggc cgcccggccg
     1741 cgtctcccgc cgtcccttac actcactgcc attgcccgga aggctgtggc ttttgcccgg
     1801 ttttcccgtc cctccagcct gttgttcact tttcccgacc ccgaaagcgc gcccatgtcc
     1861 gccgtcttca atttcacctt tgttccctgg ttccgttcgg tggcgcccta catccacctg
     1921 caccgcggca agacctttgt ggtcggcatt gccggcgaag ccattgccgc cggcaaactg
     1981 cagcacctgg cccaggatct ggcgctgatc cagagcatgg gcgtgaaaat cgtgctggtg
     2041 cacggcttcc gtccgcaggt caacgaacaa ctggccgcca agggccacga ggcgaagtat
     2101 tcgcacggca tgcgcatcac cgattcggta gcgctcgact gcgcccagga agccgccggc
     2161 cagctgcgtt acgagatcga ggccgccttc agccagggcc tgcccaacac accgatggcc
     2221 ggctccacgg tgcgcgtcat ctccggcaac ttcatcaccg cccggccggt cggcattgtc
     2281 gacggcgtgg acttcaaaca ctcgggcctg gtgcgcaagg tggacaccaa aggcatcctg
     2341 cgcacgctgg agttcggcgc catggtgctg ctctcgccct tcggtttttc acccaccggc
     2401 gaagccttca acctgaccat ggaggaagtg gcgaccagcg tggcgattga gctgcaggcc
     2461 gacaagctga tcttcgtgac ggaaattcca ggcatccgca tccaccctgg tgaacccgaa
     2521 agcgaagaca acccgatcga caccgagctg ccgctggcca ccgccgagaa gctgctcgcg
     2581 caattgccca acgccaccca gcccacggac accgcgtttt acctgcagca ctgcgccaag
     2641 gcctgcaagg ccggcgtcga gcgcagccac atcctgccgt tcgcggtgga cggcgcgctg
     2701 ctgctcgaaa tctacgtgca cgacggcatt ggcaccatgg tcatcgacga aaaactcgag
     2761 agcctgcgcg aagccaccgc cgacgacgtg ggcggcattc tgcagctgat cgagcccttt
     2821 gaaaaagacg gcaccctcgt caagcgcgat cgcaccgaga tcgaacgcga cgccgacctc
     2881 tacaccgtga tcgaacacga cggcgtcatc ttcggctgcg ccgcgctgta tccctacccc
     2941 gaagccagaa ccgccgaaat ggcggcgctc accgtgtcgc cgcaaagcca gagccagggc
     3001 gacggcgaac gcatcctcaa acgcatcgag cagcgcgcca agctgatggg cctggacagc
     3061 atttttgtgc tgaccacccg caccatgcac tggttcatc
//
LOCUS       DAIDFG010000351         3092 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_351_length_3092_cov_17.389529, whole genome shotgun sequence.
ACCESSION   DAIDFG010000351 DAIDFG010000000
VERSION     DAIDFG010000351.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_351_length_3092_cov_17.389529"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          634 a         1031 c          919 g          508 t
ORIGIN      
        1 ctgaacgacc tgtttgccgc cgccaaccag aatgccaaac acagcggccc gatggagcgg
       61 attttcgcca gcggcatgcg tgaattccag aagctgcgcg agcgccgcat ggaagacccc
      121 ggcaccctgc tcgacggcgc ccgccgcgcc atgcgtgcca gctaccagcg tgaaatggat
      181 gcggtggagt ccaacctgtc ctttctggcc acggtcggct cggtctcgcc ttacgtcggc
      241 ctgttcggca cggtctgggg catcatgcat gccttcaccg gcctggccgc gctggagcag
      301 gtcacgctgt cttccgtggc gcccggcatt gccgaagcgc tggtggccac ggccatcggc
      361 ctgtttgccg ccatccccgc ggtggtggcc tacaaccgct ttgcccacga tatcgaccgc
      421 gtggccaaca agctggagac cttcatcgaa gagttctcca acatcctgca gcgcaacctg
      481 ggtgcccatt cgccctcagg tcactaagcc agggagccgc cctcatgcca gcagtcaatt
      541 cccgcggccg cggccgccgc accatcagcg aaatcaacat ggtgcccttc atcgacgtga
      601 tgctggtgct gctcatcatc ttcatggtga cggcgccgct gatcacgccc agcatgattg
      661 cgctacccaa ggtcggcaag gcgccaggcc agccgcccaa gccgatccag attgaaatca
      721 gcaaggacga agtcatccag atcaagtcgg cgggcaacac cacaccggtc aagctcggcg
      781 aggtcggcgc caccgtcaag cgcctgcagc ctgcggtgcc ggccggtgaa caggtcacgc
      841 cggtggtaat cagcgccgac aaatccatca agtacgaaac cgtggtcaag gtcatggacg
      901 cgctgcaaaa ggccggcgtg gcccgcgttg gcctctcggt gcagaccggc aactgacccc
      961 cagcccgcgc tgaatttttt catgcccatc gccgccgatc gccccgagtt tgccccgcac
     1021 cgcgagcccg gtgcgttgcg atccttggtc ctggcggtgc tggttcacct gctgttgatc
     1081 gcagccctga cctggggcgt gaactggaag cacagcaacc cggatatctc gttcgaagcc
     1141 gaactctggt ccagcgtgcc tcaggaagcc gcgcccaaac tggtggaagc tccgccaccg
     1201 ccgccacccc ctcctccgcc cccgccggag cccgcggtca aggcaccacc acctcccccc
     1261 gcaccggacg tggacattgc gctggaacgt gagaaaaaac gcaagctcga actcaagaag
     1321 aaggaagctg aactcgcaaa agccaaggcc gagctggacc ggctgaaaga actccaggtc
     1381 aaaaaggaca aggagctcaa ggcgaaggaa gaactggcca aacgcaaggc ggaagaagcg
     1441 aagaaactcg aagcgaaaaa agccgacgac aagaagcagg acgcgaaaga cgcggaaaag
     1501 aaaaaacagg ctgacgccgc agcggaaaag cagcgccagg aaaacatcaa gcgggtcatg
     1561 ggcctggccg gtgccaccgg cggcgccgac gccaagggca cggcacaaaa agccagcggc
     1621 ccgtcagcca gctacggcgg caaggtgcgc gccaaggtca aacccaacat cgtcttcacc
     1681 gaggacatct ccggcaaccc gaccgccgag gtcgaggtgc gcaccgcact ggacggcagc
     1741 atcatcagcc agcgcctgat caagtccagc ggcaacaagg cctgggacaa cgcggtgatc
     1801 aaggccatca tccgcaccga aaccatgccg cgcgatgtgg atggccgggt gccgacaccg
     1861 atgattctgg aattccggcc caaggattga gcgccgatca cttcgaatgc tatcaataag
     1921 atagctatcc gcgcaatcaa caaaagggct ggagccttta tttactcata aacaatttgt
     1981 gcccgcccct ggtcggcctg ggccatccag ctggccagtg ccgcatcggt cgggaagaat
     2041 ttcgcggctt caccaagctg cagttcggcc atcacgctgt cgcgcttgag gctcaggcgc
     2101 accggcaggc cgcggctgac ctcgccctgc tccgacattt ccttgcgcgg cggaaagtcc
     2161 cgcacgatgc gctgcacgtc cggcgccgtg ccgttgaccg ccacgcgcag gtacttgccg
     2221 aagcggcagc gtgccgactc caggctccag acctgcgtca ccttgaagcg gcgaccgaag
     2281 cgctccgacg ccggttgcag cgtgccctgc acgatcacca gttcgtcgtc tttcagcagg
     2341 ttgcggttgg cattgaaggt ggcctcgtcc accgaggcct ccagcgtgtc ggtcttgtcg
     2401 tcgagcttga agatgatcag cttgccgcgg ttgccgttga tcacacgcag gtcgctgacg
     2461 atggccgcca gcaggtgctg gtcgcgcgaa tcgatcaggt cgtcgatcct gcgtttgacg
     2521 aactgccgca cctcggtctc gacttcgtcg aacaaatgac cggacaggta aaagccgatg
     2581 gcggtttttt cctgcaccag ccgcgccttc acaccgaagg gcacggcctc caccagcggc
     2641 ggctcctgcg tgctcgccgc atgcgagtcc atgtcgaaca ggccgccctg gttcacattg
     2701 gcttcggcct gggcggcaaa ttcaaaagcc cggtccaccg aggccagcag cgaggcacgg
     2761 ttcagttgca ggttgtcaaa ggcgccggcc ttgatcagcg cttcaaccgt gcgtttgttc
     2821 atcttgctct tgtccacacg caagcagaag tcgtacagcg aggtgaaagg cccgccggcc
     2881 tcgcgcgccg cgacgatggc cgcaatcgcc tgctggccgg tgcccttcac agcgcccagc
     2941 ccgtaacgga tgctcttgtc ggtgatgggc tcgaagcggt agtggccgcg gttgacatca
     3001 ggagattcga agctgatgcc catcttctgc gcgtcgccga aaagaatcct caatttgtcg
     3061 gtatcgccca tctccaccgt catgttggcg ca
//
LOCUS       DAIDFG010000352         3091 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_352_length_3091_cov_15.975626, whole genome shotgun sequence.
ACCESSION   DAIDFG010000352 DAIDFG010000000
VERSION     DAIDFG010000352.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_352_length_3091_cov_15.975626"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          588 a          974 c          950 g          579 t
ORIGIN      
        1 tgtcggactg gtactcgatg ctgccaaaca gcctgcgcag cagggggttg tcctcgacca
       61 tcaccggcgc gccggtcagg ccgtggttgt caaccaccag gttgacccgg taacggccca
      121 gcacggaagc caatgcctca agccggacct cctcgtcggt gtcgcccgcc tggaacagct
      181 ccaggttttc gagcacatcg tgcatgacca gttccaggta gttgcccaac ttgaccgagt
      241 ccttgatctg tttgcgcagc tcgttgcgga tctcctgcag ttcgtgctcc agcatcggtt
      301 tgaccacctg gcggcgcaag gccgccaggc cttcatgcag gacccgttcc atggagcggg
      361 ttttatcgag aaaccgggcg atttcggcac gcagctcctg ctcggcgcgg tcaatctcca
      421 cacgccgctc tttcggcagg gccagcgcct cgctttcagt caaggcccgg cccttgtcgt
      481 ccctcagggt gaagaccagg tggcccgcct cgcgaaacaa tgaaaagctc cgggcctcgg
      541 caaacgcatc cagctccgca taggccgggt cctcttcagc cttgtaactt ttttcgatgc
      601 gttcgctttc cgtgcggaaa tcctgatcca ccaggcgttt tggaatttcc gcctgaagcg
      661 ttttggtcaa ctgcagcatg agctggcgca gcagccggcc ctgcccggcc ggcatgcgca
      721 aggcccgggg ccgctccggg gcctcgaaat tgttgagata acacaggtcc ggcggcaccg
      781 gccggctggc cgccgccgtc tgcatcatct gcagcagcag ggtcgatcgt cccgtgccga
      841 cctcgcccag cacgaaaagg ttgtagtcgg gttgcgccat cgccaggcca aagcgggccg
      901 cggtttcggc acgctcctgg ccgatccagt gcatcggctg gtccagcagt tcggacgtgt
      961 ccgcaaaccc caaaagcccg gggtcgatgg cgagacgcag atcagcggga aggactttgg
     1021 caacaggcat ggcgggttcc ggcaaggggg aagaatggcc ggattttgcg cttagctgac
     1081 cagctggcgc atccagtcgg gcaaggcggc ggccttctgg ctcggaaagt cgacccagat
     1141 cgtcgtggcg ccgccggcgg catagatcac cccgggctgg tcggcgcgct ccatcgtgcc
     1201 ccagctctcg aaggtggtgc gtgccgggtc gctgacgtac atcttgacca gtacgtcgcc
     1261 cgggtattcg agctgtttgt agaaattgca gaacgcattg acgatgaccg ggccttcgcc
     1321 ctgggggtcg ggcacacagc cgatggaatg catccagtcg atgcgaaccg tttccaggta
     1381 acggaaatag ctggtgttgt tgacgtggcc catggcgtcc atatcgcccc accggatggg
     1441 gacacgcatt tcgaagacca gtttcttgat gtcgggaatt tcaattttca tggcgctgtg
     1501 aagacgagga gagtcgaaga aaaaggacaa atgaaagatg gccgatggac aagcgctcag
     1561 cggcgcaggc ctcaaagccc gaagccgtca tccgccgcaa tgacagcgcc gttgatgaag
     1621 tggctctggc cgctggccag catcaccagc aacgcgtcca ggtcctccgg cttgccgatg
     1681 cgcttgcgcg gcagcatctg caccagccgt ttgccctgct cggtttccca gtggtggtgg
     1741 ttgatctcgg tgtcgatgta gcccgggcag atggcgttga cgttgatgtc gaacttgccc
     1801 cattccagcg ccatggcttt tgtcatctgc accaccgccg ccttgctcat gcagtacacg
     1861 ccgatctgcg gcaacacgcg caggccggcc atggaggcaa tgttgatgat gtgcgcgccg
     1921 gaaaaggtgc cgggcgccgc gccgcgcgac cgcgccagca tgcgtttgcc gacttcctgc
     1981 gccacaaaaa aggcgccctt gacgttggtg ccgaagatga agtcgtagtc ttcctcgccc
     2041 acgtcctgga tgcgctgcgt ggtgctcacg ccggagttgt tgacgaggat gtcaatggga
     2101 ccgacctcgg tctcggcgtg cgccaccgcc gacttgatgc tggcatggtc ggtcacatcg
     2161 agcgacacca catgcgcatc accgccttcg gcctcgatct gcgcgcgcaa gtccttgagc
     2221 ttgtccatcc tgcggctggc cagcaccacc gctgcgccgg cacggctcaa ggtcttggca
     2281 aactgcgcac ccaggccgcc ggatgcgccg gtcacgagcg cgacacgccc tgaaagatcc
     2341 aggctgtaag ccatgcgagg ctcctcttga taaaagtatg ccgatgcgaa cgatcgttcg
     2401 aatcagcgga ttcacgacat aaaatcaggc gcaagacctg cggccgtcaa ccgacgggca
     2461 gccattcctg aaattcatta tcagcgagtc cacgatgaac aaccaagaaa tactggccca
     2521 gttcggcccc cgcgaagcga tggaatacga cgtggtcgtg gttgggggcg gccccggcgg
     2581 tctggccacg gccattcgcc tgaaacagct ggccgcagaa aaaggcgcgg agctttccgt
     2641 ggtggtgctt gaaaaaggct ctgaacccgg cgcgcacatt ctctccggcg cggtcatgga
     2701 ccccaaggcc ctcaccgaac tgatcccgaa ctggaaagaa cttggagccc cgctcaacca
     2761 gcccgtgacc agcgacgagg tgctgttcct cagcgaaaca ggtgcgacca aaacaccggc
     2821 gttttttgtg cccgaatgtt ttcacaacga aggcaactac gtggtcagcc tcggcaacgt
     2881 ggtgcgctgg ctggcgcagc aggccgagaa cctcggcgtc gagattttcc ccggctttgc
     2941 agcggccgaa gtgctttaca acgacaacgg ttcggtcaag ggcgtggcca ccggcaacct
     3001 cggtgtcggc aaggacggcc agcccagcga ccatttccag ctcggcatgg aactgcatgg
     3061 caagtacacc atctttgccg aaggcgcgcg c
//
LOCUS       DAIDFG010000353         3076 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_353_length_3076_cov_22.560741, whole genome shotgun sequence.
ACCESSION   DAIDFG010000353 DAIDFG010000000
VERSION     DAIDFG010000353.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_353_length_3076_cov_22.560741"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          531 a          951 c          961 g          633 t
ORIGIN      
        1 ggcgccgcgg ttctggttga aggcattgac gcggaaacga gccaggccgt caatctcgaa
       61 ggagaagtcg atctccagga attcttcgta gttcttgcgc tgggtgtcgt tcatgatgtc
      121 gtacaccatg gcatgaacct gcttgtggtc cagcgggtcg acattgatgc gcctgacatc
      181 cccgtgcacc cggatcatgg gcggcaggcc cgctgacaga tgcagatcgg acgccttgtt
      241 cttgacgcta aaagccagca gttgggtaat gtccatcccg gggatccctg aaaagtttta
      301 cagttggatt atgaccatga ttgtgcgcaa gctccaactt gtccgtgacc gtataaccac
      361 ggcctgtgtc gctgccggac gcgaccccgc cagcgtgcgc ctgctggccg tgtccaagac
      421 cttcggtgcc gatgcggtga tcgatgccat gtccgatggg caatacgcgt tcggcgagaa
      481 ctacatccag gagggtgtgg acaagattct cgcggttcgc ggatgggaag ccgatcaccc
      541 cggcctgcca ccgtcccgcc catccgagtg gcattgcatc ggccccctcc agagcaacaa
      601 gacgcggctg gtcgccgaac atttcgactg ggtgcagtcg gtggaccgcc tgaagattgc
      661 gcagcgcctg agcgagcagc gtccgccgca tttgccgccg ctgcaggtgt gcctgcaggt
      721 caacatcgac ggcggcgcca ccaagtccgg cgtgacgccg caggacgccc aggcgctggc
      781 ggccgaggtc gcgcggctgc cgcggctgca gctgcgcggc ctcatgtcga ttcccgagcc
      841 tgctcctgat tttgtagctg cttgcgctgt ccataaaagg gttgcagacc tttttgacca
      901 gatcaatcgg gttggtgtgt tgccgcaggc gatggacacg ctgtcgatgg gcatgacggc
      961 cgatctggaa gcggccattc atgccggcag caccatggtt cgggtgggga cggcgatttt
     1021 cggcgacaga tagagccccc acgttcgttc actgcgtgta actctctgcc ccccgagggg
     1081 gcgctgcgcc tgcggcccgg caaagccggt tccgcggccc ctgctggaat gaagaagggc
     1141 cttgcagctc ccgcgcgccg tgggggctat ttcttcggtg cggcgagggg cttgatgttt
     1201 ccgcagtccg ctgacagcca cttgccgccg ccgtccatgg tcatggtttc gggtttgccc
     1261 tgcatcatgc ttttcatggt catcttcatc gtgtaagcct cggggctgac aaaggtgacc
     1321 gtgccttcgc cgctggacgg gggctgggtg caggcaaacg acatcttcat ggtgttgccc
     1381 gaacgcggcg agttcgtggt tttgcagtct ccctgctggg cgggcagctc attgcgttgc
     1441 gccatctctt tggtcatgca gattttcacg gccatgttgc ctccggcacc ggggcccatg
     1501 gatacaccct gtttggccat catgtcctgc atcatcttgc gctgctcggg cggcatggcg
     1561 gccatctgct tttgcgcctc ggccatggct ttttccatct gccccgagct ggactgcatt
     1621 ttgttgttga tctcccacaa accgggcttg agagtctggg ccgctactgg ctgggcggcg
     1681 agggcaagca gtgccagcgc ggcaccactc caaaggcgat gaggtgtcag gtctttcatg
     1741 gcgggtcctc ccaaaaaaac atcactatag ggggaacagg ccctatcgca caaaacgtcc
     1801 gccacgccca atgatggtca ggtcggtgta atgggatgct tcatggttct tcgggctgta
     1861 actgaccagg aacccgccca tgttgacatc acgcatggac tccagcccgc tgaccaggct
     1921 cgcgcgtgtc agggccttgc cggcccggcg caccccttcg acaaacacct tggcggccag
     1981 aaagccttcc atgctggaga agtcgaagtc cttgttgccc gcttcggtca tgcgctgctg
     2041 gtactcgcgc acgatctccg acgaaggcgt gtagggaaag ggcaccacct gcgaaatcgt
     2101 cacgccggcg ccggcatcgc ccagttccgc cgacagggcc gtggagccga caaaactcac
     2161 attgaaaaat tgtccaccgt aacccttgct gcgcgccagc ttgatcagcg ccgcacatga
     2221 tttgtaagcg ctgatctgca ccaccgcttc cggggcggct ttcacgagtt cggcccatgc
     2281 ggcgctgaca tcgaccgaat tgcgttccac cgtcacggct gcggcgggct tgaggctgcg
     2341 tttggtcagc gcccgcgtta cgccttccag gccggctttg ccgtaggagt cgttctggta
     2401 gaaaaccgca atcttcttga tgcccagcgt gctcaggtgc tgcacgatgc gttcggtctc
     2461 gtcaaagtag ctggcgcgca catgaaacag gttgcggttg agtggttcgc gcagggcctg
     2521 cgcgccggtg aagggaccga tcagcggaat gccggccgca ttgatggcgg gcaccgccgc
     2581 cagcgtggtc ggcgtgccga ccgagcccac cagcgcaaac accttgtctt cctcgatcag
     2641 ggccttgacg gcggccgccg ccttctccgg ttcatacccg tcgtcgcgcg ataccagctt
     2701 gatggtgcgg ccgttgatgc cgccggccgc attgaccgca ttgaaatacg ccagcatgcc
     2761 cagctgcatg cgtttgccca gttcggcggc ggggccggtc tgggccgcga actgccccac
     2821 caggatttcc ttgtccgtga cgccgatgtc cgccgcatgg ccgggcgccg agagcaggag
     2881 tgcaagcagg ctgcaggcac cccatcgttg gaaaaagttc atgctggact ccgtggcgtt
     2941 gttggacgat gcgggcgggc tgcctatgca aagcagaacc tacccgaagc aaacttattc
     3001 caaatagccg gcggtggcaa tgcggtttgc ccacaaggca tgaactcttg ggtcaaacag
     3061 cgcaaatagt cccgct
//
LOCUS       DAIDFG010000354         3069 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_354_length_3069_cov_18.159257, whole genome shotgun sequence.
ACCESSION   DAIDFG010000354 DAIDFG010000000
VERSION     DAIDFG010000354.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_354_length_3069_cov_18.159257"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          551 a          954 c          971 g          593 t
ORIGIN      
        1 cagcgtgccg tcccgcatgt agtgctggat gtccttgagt cccgccccgg cttcctccgc
       61 aggcgacgca ctgacaacag gaatggggaa ggtcatgaga agaaagcgga aacgctcaca
      121 accagcgctc gcgcatcagg tccatcatct ggcgcgccgc catgccgcgg tggctgttgg
      181 cgtttttaac ctcgaccggc aattgcgcaa aggtcttgcc gaaggccggg atgtacatca
      241 ccgggtcaaa accaaaaccg ttgctgccca ccggctcgcg cgcgatctcg ccgctgacac
      301 ggcccaccgc aatcaggggc tcggggtcgt cggctgaacg cacggccacc agggtgctga
      361 caagcgcggc gcggcgctgg tccaggccga ccatctgttc gagcagggcg cgcacattgt
      421 tgtcgtcgcc tttttcgtag ccaaactgcg tggcgtaaaa agccgtgtcc acaccgggca
      481 ggccgccaaa ggcatcgaca cacaggccgg cgtcgtcggc cagcgccggc aggccgctga
      541 gccgtgatgc atggcgcgcc ttggccaggg cgttttcgac aaaggttcga aaaggttcct
      601 cagcctccgg gatgcccagg tcggcctgtt tcaccagtgt cacgcccagc ggcgccagca
      661 tggcctgcag ttcggccagt ttgccggggt tgttcgatgc aagtacgatt ttcataagca
      721 aaatatggct tcagcccaat caggacgggc gcaagcagct attaattcag gagcgactgc
      781 tgctgcagcg tgaccagctc gctgatgcct ttttcagcca gccgcagcag cgcgtccatt
      841 tcctggcgcg aaaaggcggc tccttcggcg gtgccctgca cttcgacgta gtggccggcg
      901 ccggtcatca ccacgttcat gtcggtatcg cagctcacgt cttcaatgta ttcaaggtcg
      961 agcaaaggcg tgccctgcac gatgccgaca gaaatggcgg ccacgtggcc gatgatcggc
     1021 gtctccttga gcttgccttc ggccagcagc ttgttgacgg cgtcctgcgc cgcgacaaag
     1081 gcgccggtga tgctggccgt gcgtgttccg ccgtcggcct ggatcacgtc gcagtcgagg
     1141 tgaatggtgc gttcgccgag ttttttcaga tcgaacaccg cgcgcatgga gcggccgatc
     1201 aggcgctgga tttcctgcgt gcgcccgctc tgcttgccgc gcgcggcctc gcggtcgctg
     1261 cgggtgtggg tggcgcgcgg cagcatgccg tactcggcag tgacccagcc ctcgccgctg
     1321 cccttcttgt ggcccggcac tttctcctcg accgaggccg tgcacaacac ccgggtcgcg
     1381 ccaaactcga tcaggaccga gccttccgca taggcggtgt aggcacgggt gatgcgcacc
     1441 gggcgcaact ggtcggcggc acgggcgctg cttcgcgaaa atgaactcat ggtcagggct
     1501 ttctggcgtc actgcgcccg gggaaacaga aaaaaaagaa aagggaagtc atgccaaagg
     1561 cagcacctgc gcgaaggcgg ccaagctgcc aggcggggaa ctcaggcctt ttttgccgca
     1621 gagcggcgta tcgcctcgtt gatttcggca atggagcgtt caatcgcctc atcgtcaagg
     1681 tcttcgacgc tggagtcgag cacgcctgcc tggatggtcg acgcaaacac gccgtccatg
     1741 atgctgtcgg tcgaaacgcc gcgcgtcgat tcgaaagcgt cgggcgtttc ccactccatg
     1801 gccaacacgg tgacgttgtc gctgtgctcg ccaccattgc gcagcgcatg ttcgaccatg
     1861 tcgggcacgg cgtcagacac gctgttgcca gccatgtggc gcacaatttc agcgtcggtc
     1921 agcgtgcccc acaggccgtc ggagcagagc atcagcttgt cgccctgctg caggaccacc
     1981 gggccggcga tatcgaacac cggcttggac ggggaaccca ggcaggtgaa caggatgttg
     2041 cggttcaggc gctcgagttt gatgatgccg gcgttttgct gctccaggta ggagtggtcg
     2101 cgcgtgcggg tcaacagctc gccgtcgcgc accacgtaca gccgcgagtc gccgcaatgg
     2161 acccaggtcg cgcccgagcc ctgcaggatg gccgccacca ccgtggtccg gggtgtatcg
     2221 agcatgcctt tttcgctggc ataacggatg atctggtggt gcgccgccat cagggccgac
     2281 gagaggaatt cggtggtgtc cctgaccagt ggccgggctt ccttttgata cagcgcagaa
     2341 atggtctgca gcgccagctg ggccgccacc tcgccttcgg ggtggccacc catgccgtcg
     2401 gccagcacaa acaggcccga ctcgcgcgtg tagcaatacc ccatgcggtc ttcgtttttc
     2461 tcgcgaccgc ccttgcgact gacctgaaag accgagaatt tcatttgaat ttcgtcgcaa
     2521 agccggtggc cttgcgcgcg gttttcttgg tgtctgacac catgttgtca aactgcagcc
     2581 gcattttttc gccgacggtc agcttggtgt agcgccgctc gccttcgcgg ctgagttctt
     2641 tctgcagggc aaaaaccgac tgcggccgcg acaggggatc gagtgacatg caccattcaa
     2701 ccacttcaat gaggttgtcc gaatacacac cgcgcaaacg ggagagtgca agcgacagac
     2761 ggtctttttc gagccgctgc ggcgcgtcat tgggcggata accctgcatg gtcgcgtaaa
     2821 tacaggcgcc gatcgcgtag atgtcggtcc acggtcccat ggaggaatca cgccggtaca
     2881 tctcgggcgc ggcaaaaccg ggggtgtaca tgggccggat gaagttgcct tccttgctca
     2941 gcacctcacg ggcggcgcca aagtcgatca tcacggcctt gttgtcgtcg gtcacgaaga
     3001 tgttggccgg cttgatgtcc aggtgcagca tcttgtgctg gtgcacgatg cgcaacccgc
     3061 gcaggattt
//
LOCUS       DAIDFG010000355         3048 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_355_length_3048_cov_17.579018, whole genome shotgun sequence.
ACCESSION   DAIDFG010000355 DAIDFG010000000
VERSION     DAIDFG010000355.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_355_length_3048_cov_17.579018"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          512 a          915 c         1016 g          605 t
ORIGIN      
        1 atgcggatgg cgttgcggct gtagtcgctg aaggtcagga aggtgccgcc gtaagggatg
       61 tagccgccgt gcagggcgat gccgttcatg atggcggcca tgccgaattc gcgcacgccg
      121 tagctcatgt ggttgcccca gtggtcgcgt ccggccttga cgcagccctt gaagttggtc
      181 aggttggagc cggtcagatc ggcgctgccg ccaaagaatt cgggcaccag cggcgccagc
      241 gcgtcgagcg cgttctggct cgccttgcgg gtggccacgg cctcgggttt ggcggcgatg
      301 gccgccagca gcccgggcag tttgtcggca aaagcgggca gcagatcgcc ggccacacgg
      361 cgctcgaact cggccgcctc cagcgggtac tgcgtgcggt aggcctcaaa acgcatgcgc
      421 caggcacgct cggccacctg gccgcgttcc acggcattcc aggcgctggc gatgtccgcc
      481 ggcacttcga agggcgcagc agtccagccc agtgcttcgc gcgtggcagc aacttccgcg
      541 gcgccgagtg cggcgccgtg cacgtcgtgc gtgccggcct tgttgggcga acccatgccg
      601 atcacggttt tgcagcagat caatgttggc ttgccatgcg gcaacacggc ctgggccttg
      661 gcggcacgca cggccgcatc cagcgccacc gggtcatgcc cgttgacagc ggggatgacg
      721 ttccagccat aggcctcaaa gcgcttgggc gtgtcgtcgg tgaaccagcc ttccacatgg
      781 ccgtcgatcg agatgccgtt gtcgtcgtag aaacccacca gcttggacag gcgcagcgtg
      841 ccggccagcg agcaggcctc gtggctgatg ccctccatca ggcagccgtc acccatgaac
      901 acataggtga aatggtccac gatggtgtgg ctggtttcct cgtcttcgcg gttgaactcg
      961 tcggcgagca gcttctcggc cagtgccatg cccaccgcat tggtgaggcc ctggcccagc
     1021 gggccggtgg tggtctctat cccgggggtg acgcccactt cggggtgacc ggcggtcttg
     1081 ctgtgcagct ggcgaaattt cttcagttcg tccatcgaca ggtcgtaacc ggtgaggtgc
     1141 agcagcgcat agatcagcat cgagccatgg ccgttgctca gtacaaaacg gtcgcggtcc
     1201 ggccagtgcg ggttggccgg gttgtgcttg aggtgccggt tccacagcac ctcggcgatg
     1261 tcggccatgc ccatgggcgc gccgggatgg ccggatttgg ctttttcgac cgcatcgacg
     1321 gcgagcatgc ggacagcgtt ggccatttgt ctggccatgg cgggtacagg tgcgttcatg
     1381 tcaggaagcc ctcttttttc gttccatcat ccgccacaca aagggcgtga agatcaattg
     1441 catggccagc tccatcttgc cgccgggcac cacgatggtg ttggcacgcg acatgaacga
     1501 gtcgttgatc atgctgcgca ggtactggaa atcgatgccc ttgggattgg cgaaacggat
     1561 caccaccatg ctctcatcgg gcgacggaat gtcgcgcgcg atgaaggggt tggaggtgtc
     1621 caccatgggc acgcgctgga agttcacgtg cgtgtgcatg aactgcgggc agatgtagtt
     1681 cacgtaatcc ggcatgcggc gcaggatggt gtcggtcacg gcctcggtgc tgtagccgcg
     1741 cttggacttg tcgcggtaca gcttctggat ccactccagg ttgatcaccg gcaccacgcc
     1801 gatcagcagg tccggatgct gggcaatgtt gacctcgggc gtgacgacgg cgccatgcag
     1861 gccttcgtag aacagcaggt cggtgccggc aggaacgtct tcccaggccg tgaaggtacc
     1921 gggctgctgc ttgtagggcg ctgcctcttc cgtgtcgtgc aggtacttgc gccgcttgcc
     1981 ggtgccggtt tcgccatagg cgcgaaacag ctcggccagc tcgggaaaca ggttgttctc
     2041 cggcccgaaa tggctgaagt tgttgttgcc cgcggcctcg gcctcggccg ctttggtttt
     2101 catgtccttg cggtcgtagc ggtggaagct gtcgccttcg atgatggcgg cattcacatt
     2161 ctcgcggcga aagatgttct cgaacgtgtg cgtgaccgag gtggtgccgg cgccggacga
     2221 gccggtgatg gcgatgatgg gatggcgttc agacatgggg gtgtctccgt aagtcaaaaa
     2281 aatttggcaa aaagtgcctg taacccttat ggttgctgcg ttatcagcta tttattcaat
     2341 agcacttcgg cagccgccac cgaccatcag acgcggaaca ggctgcgttc ggcaaacagc
     2401 ggattggtct gctcggtctt ggcgggctcg gcgtggtaac gctcaatgcg ctccacctcg
     2461 ctcctggagc cgaagaccag gccgatgcgc tggtgcagcg agctcggctt cacgcccagc
     2521 atgggctggt gcccggtcga agcccggccc ccggcctgct ccatgagcat gcctatcggg
     2581 ttggcctcgt acagcagccg caggcggccg ggtttggagg cgtctttggt atcgcgtgga
     2641 tagaggaaga caccgccgcg catcagaatg cggtgcgcct cggccaccat gctggcaatc
     2701 cagcgcatgt tgaaatcctt ggcgcggggc ccggtcttgc cggccaggca ctcatccaca
     2761 taccgcttga ccggcgcctc ccagaagcgg ctgttggagg cattgatcgc gaactcctgg
     2821 gtatcggctg gaatctgcat cttcggatgt gtgagcatga attcgcccag gttcggatcc
     2881 agcgtaaacc cattgacgcc atcgccgacc gtgagcacca gcatggtggt agggccatac
     2941 aaggcgtaac ctgcggccac ctggctggcg ccgggctgaa aaaaatcagc ctcggtcaca
     3001 tcgcgaccgg tgtcttccac gtcttgcggt gcgcgcagca ccgaaaaa
//
LOCUS       DAIDFG010000356         3026 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_356_length_3026_cov_19.518681, whole genome shotgun sequence.
ACCESSION   DAIDFG010000356 DAIDFG010000000
VERSION     DAIDFG010000356.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_356_length_3026_cov_19.518681"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          598 a          941 c          982 g          505 t
ORIGIN      
        1 tcggcggcag cctggccaaa atccccgcga ccgaactcgg tgcggccgtc atcaaggcgg
       61 tcgccgagcg cgccggcctg tcggcggacc tcatcggcga agtcatcatg ggccaggtgc
      121 tggcggccgg cgtgggccag aacccggcgc ggcaaacggt ggtgaaagcg ggctttccgc
      181 acggcattcc cggcctgacc atcaacgccg tctgcggatc cggcctgaag gccgtgatgc
      241 tggccgcgca ggcggtggcc accggcgaca gcgagatcgt gattgccggc ggccaggaga
      301 acatgagcgc cgcgccgcac gtgctcaacg gctcgcgcga aggccagcgc atgggcgact
      361 ggaaaatgat cgactccatg atcgtcgacg gcctgtggga cgtctacaac cagtaccaca
      421 tgggcatcac cgccgaaaac gttgcgaaga aatacagcat cagccgcgag atgcaggacg
      481 agctcgcgct ggccagccag accaaggcca tggcggcgca ggacgcaggc aagttcaagg
      541 atgaaatcgt gccgttttcc atcctgcaga aaaaaggcga tgccgtcgtg tttgcggccg
      601 atgagttcat caaccgcaag agcagcgccg aaggcctggc cggcctgcgc cctgcgttcg
      661 acaaggccgg cggcgtgacg gcgggcaatg cctcgggcat caacgacggc gcggccgctg
      721 tcatggtgat gacggccaaa aaggcggcgg cgctgggcct caagccgctg gcccgcattg
      781 ccagctacgc gaccagcggg ctggacccgg ctttcatggg catgggcccg gtgccggcgt
      841 ccaccaaggc actggcgcgt gccggctgga aggcgcagga cctggacctg ctggagatca
      901 acgaagcctt cgcggcgcag gcctgcgccg tcaacaagga gatgggctgg gacaccagca
      961 aggtcaatgt caacggcggt gcgatcgcca tcggtcaccc gattggtgca tccggttgcc
     1021 gcattctggt gacgctgatc cacgagatgg tccggcgtga tgccaaaaaa ggcattgcca
     1081 gcctgtgtat cggtggcggc atgggggttg ccctcacact ggaacggtaa atatgaggta
     1141 aaagagcttg tgacccttgt agggtgcgcg cgaccagcta tttatttgat agcaagtttc
     1201 aaacaccggc aagctcacca aagcttcttt tcaacggatt tcagaaccaa taaatcaagg
     1261 agcaagacat ggcaaaacga attgcatacg tgactggcgg catgggtggc atcggaaccg
     1321 ccatctgcca gcgcctgcac aaggacggct tcaccgtggt ggccggttgc ggcccgacgc
     1381 gtgacttcac caagtggatt gacgagcaga aggcgctggg ctacaccttc tatgcctccg
     1441 tcggcaatgt cggtgactgg gactccaccg tcgcggcctt cgacaaggtc aaggcagacc
     1501 atggcccggt cagcgtgctg gtcaacaacg ccggcatcac gcgcgacggc cagttccgca
     1561 agatgagcaa ggccgactgg gacgcggtga tctccaccaa cctcgacagc atgttcaacg
     1621 tcaccaagca ggtgatcgaa ggcatgatcg aaggcggcta cggacgcatc gtcaatatct
     1681 cgtcggtcaa cggccagaag ggacagttcg gccaggtcaa ctactccacc gccaaggccg
     1741 gcctgcacgg cttcaccatg gctctggctc aggaggtggc cgccaagggc gtgaccgtca
     1801 acaccgtgag ccccggctac atcggcaccg acatggtcaa ggccatccga cccgatgtgc
     1861 tggagaaaat cgtggccggc gtgcccgcca aacgcctggg cacgcccgaa gaaatcgcct
     1921 ccatcatttc gtggatcgcc tcggacgaag gtggttacgc caccggcgcc gacttctcgc
     1981 tcaatggtgg tttgcacatg ggttgaggct tccggcctgt caccccccaa aggaaaaccc
     2041 gcttcggcgg gttttttatt ttctgttttt ataatgaaaa cgagctggaa agccttcaac
     2101 ccccaattcg ttctgcggca agcgcctgtc cctggcaagt gctcacgcag ggtcccttcg
     2161 ccgtctctct gaaggagccc ctcccgcatg ccgcacagcg tctccctcat cagcaccatt
     2221 gccgcgcgca tgaagctgcc ctcgctggtg gacgaggccg aggcgaaacg gctgcagagc
     2281 gaaaacgccg gcaagatttt cctgggcgag gaagagctgg cgctgtcgat gatgcggtat
     2341 gtgctggagc ggtcagctct cgcgcaacgt cacacatgac cccgccctgg accatctact
     2401 gcgacggcag cgccgtcccc aaccctggcc gcatgggcct gggcgcggtg ttggtcgcgc
     2461 ctgacggcac gcggcacgtg ctttcgatgg ggaccgatgc gcggggctgc aacaacgagg
     2521 cggaggcgag ggcgctgatg gcggcgctgc gtgacgcgca ggcgcgtgga gcaacggcgc
     2581 tgctgattta cagcgacagc agtttgctgg tggagcagtt gggttctgca ggcgctgccc
     2641 ccgtggtgcg gctggctggc ctctatgaag aggctggcgc gctgctcaat actttcactc
     2701 acacctcgct gcactggata ccgcgccacc gcaatggcga ggccgatgcg ctggctcgcg
     2761 cagccctggg cctcctgccc aagaggcctg cgaagtcacc gaagaagggg cgaagatagc
     2821 tcccggcatc catgccgggc tgccacatgc gctggtgtca gagcacctcg ctggcgaaat
     2881 cagccaggcg tgaacgttca ccgcgtgcca gcgtgatgtg cccgccatgc ggccagcctt
     2941 tgaagcggtc cacagcgtag gtcatgccgg acgaaccttc ggtcaggtaa ggcgtgtcga
     3001 tctgcgcgag gttgcccatg cagatg
//
LOCUS       DAIDFG010000357         2958 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_357_length_2994_cov_14.246002, whole genome shotgun sequence.
ACCESSION   DAIDFG010000357 DAIDFG010000000
VERSION     DAIDFG010000357.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_357_length_2994_cov_14.246002"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          546 a          982 c          958 g          472 t
ORIGIN      
        1 tgtcgaactc atgatgttcg cccaggccaa cagcgaacat tgccgccaca agattttcaa
       61 cgccgatttc acgatcgacg gcgtggccca ggacaaaagc ctgttcggca tgatccgcaa
      121 caccgaaaaa ctcaacccgc aacacacggt ggtggcctat tcggacaacg cctcggtcat
      181 ggaaggcctg ccggtccagc gcttctatgc caaatcggcc tccagcccag aaggggtggg
      241 cgctaccagc tatcaaagtt atagcgctac gaacgatgtc ttgacccatg tgctgatgaa
      301 ggtggaaacg cacaaccacc cgacggcgat ttcacctttc cctggcgcat cgacgggtgc
      361 cggtggcgaa atccgcgacg aaggcgcgac cgggcgcggc tcccgcccca aggccggcct
      421 gaccggtttt accgtgtccc gcctgtttga cggcaagggt gaggggtatg gcaagccgga
      481 gcacatcgcc agcccgctgc agatcatgac cgagggcccg ctgggcggcg cggccttcaa
      541 caacgagttc ggcaggccga atctggccgg ctacttccgc gagtacgagc agaccgtggg
      601 cggccagcgc tggggttacc acaagcccat catgatcgcc ggcggcgtcg gcacgattga
      661 cgccggcctc acgaagaaaa tcctgttccc ggctggcacc ttgctgatcc agctcggcgg
      721 gcccggcatg cgtatcggca tgggcggcag cgcggccagt tccatggcct cgggcgccaa
      781 tgcggcgcaa ctcgacttcg actcggtgca acgcggcaac ccggagatcg agcgacgtgc
      841 ccaggaagtc atcaaccagt gtgcccagct cggcagcgcc aaccccatcc tggccatcca
      901 cgacgtgggc gccggcggct tgtccaacgc cttccccgaa cttgtcaacg acgccgggcg
      961 cggcgcacgt tttgatctgc gtgccgtgcc actggaagaa agcggcctgt cgcccaagga
     1021 aatctggagc aacgaaagcc aggagcgtta tgtgatggcg attgcgccgg aatcgctgcc
     1081 gcagttccag gctttttgcg agcgcgagcg ttgcccgttt gcggtgattg gtgtcgcgac
     1141 ggaagagaaa caactggtgc tctccgacaa ggaggccgca gccccagtgg acatgccaat
     1201 gaacgtgctg ctgggcaaac cgcccaggat gctgcgcgac gtcaagtccg tgcgcaatga
     1261 gatcaagccc ctggccctga caggcgtcga cctgcagaaa agcgtgatcg ccgtcctgag
     1321 ccacccgacc gtggcctcca aacgtttcct gatcaccatc ggcgaccgca gcgtgggcgg
     1381 cctgacgcac cgcgaccaga tggtcgggcc ctggcaggtg ccggtggccg actgtgcggt
     1441 gacgctggcc gattaccagg gttttgccgg cgaagccatg agcatgggag aacgcacgcc
     1501 gctggccgtc gttaatgccg ccgcctccgg ccgcatggcg gtggccgaag ccatcaccaa
     1561 cctgctggcc gcgccgatcg agctgccgcg tgtgaagctt tctgcgaact ggatggccgc
     1621 ttgcggcgag ccgggccagg acgccgcact gtatgaaacc gtcaaggcgg tcggcatgga
     1681 actgtgcccg gccctgggcg tgtcgattcc ggtcggcaag gattcgctgt ccatgcgcac
     1741 ggtctggagc gacgatggcg ccagcaaaaa ggtcacctcg ccggtatctc tgatcgtcag
     1801 cgcgtttgcg acgctggccg acgtgcgcgg cacgctgacg ccgcagctcg acgcatcgga
     1861 ggccgacagc acactgatcc tgatcgacct cggcaagggc caggcccgca tgggcggctc
     1921 catcctggcg cagacactcg agctcgaaga cggcgccgtg ccggacctcg aggacccgca
     1981 agacctggtg aacctggtca aggccatcaa tgccttgcgc gcccaggggc aggtgctggc
     2041 ctaccacgac cgcagcgacg gcggcctgct ggccacggtc tgcgaaatgg cttttgccgg
     2101 ccatgtgggc gtctcgctca acgtcgacat gctgctgctc gaaggcgacg gcatttccga
     2161 cagccgcatg gacaccggcg acagcaagaa ctgggcgtcg caggtcggcg cgcgccggga
     2221 agagctcacg ctcaaggctc tgttcaacga agaactgggt gtcgttatcc aggtgcggac
     2281 cgaggcacgc aatgccgtga tgcagaccct gcgcgaacac ggcctgtcca gattcagcca
     2341 cttcatcggc aagacccggc cacaacatgc ctcgcttgag gtgggcaagg gcgagatcca
     2401 ggtctggcgc gataccaagg cggtcttcag cgcgaagctg caggacctgc accaggtctg
     2461 ggacgccgtg agctggaaaa tcaaccagca gcgcgacaac ccggcctgcg cagatgccga
     2521 acatgcggcg gccggcgatc cgctggaccc cggcctgcac gtccatctga cgttcgaccc
     2581 ggccaagcgc ccgcaggcgc cggcgctgaa cctgtcgcgg ccccgggtcg cggtgctgcg
     2641 cgagcagggc gtcaattcgc atgtggaaat ggcctacgcc ttcacccagg ccggcttcga
     2701 ggcctgcgac gtgcacatga ccgacctgca gaccggccgc gcgcagctgg ccgacttcac
     2761 cggcgtggtg gcgtgcggcg gtttcagcta cggcgacacc ctgggcgctg gcatcggctg
     2821 ggcccgttcc atcaccttca accccgcgct gtcggaccag ttccaggcgt ttttcgggcg
     2881 ccccgacacc tttggcctgg gcgtgtgcaa cggctgccag atgtttgccg agctggccga
     2941 catcattccc ggcgccga
//
LOCUS       DAIDFG010000358         2992 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_358_length_2992_cov_18.871297, whole genome shotgun sequence.
ACCESSION   DAIDFG010000358 DAIDFG010000000
VERSION     DAIDFG010000358.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_358_length_2992_cov_18.871297"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          474 a         1014 c         1043 g          461 t
ORIGIN      
        1 tcacggtgtt caggagaaaa cagcccgcca atggccacgc tgcaggtcgg ccagcccggc
       61 gccgggcagc ggtgccgtct ccccggctgg caatcgcagg aagcttgcaa tcttgagctg
      121 tcccgggtcg gcccggtctg gcttgccgct gctgccagcc gtcgcgcgtg aggcgctata
      181 aaatcagtag ctggtggcgc ttgtccagaa agggctgagg gccgatttga cctgaaaaac
      241 tcagatgccg gaaggttttt tcagggtctg caggcggtcc tgcgctttca gcacaatggt
      301 cttcacgtcg gcgccctgca gcacggtcag ggacacctcc gcatcgggtg cggcccggtc
      361 ccagagtttt ttgtagaaat cggccagcgt cgcgaccttg ctgccgtcca ccgccagcac
      421 cacgtcaccc gcctccaggc cggccagctc ggccgggctg tccctgctga cacgcacgat
      481 ctggatgcgt ccgccctggt cgctcgaatt caggcccagc cagggccggc ggctttgggc
      541 gctggtgccc gaacgctgca tttcggccag gatggggtgc agcaggtcca ccggcacaaa
      601 catgttgccg ggcagacgct ggttctgtcc gctggcgtcc gacaccagca gcgagccaat
      661 gcccaccagt tcacctttct ggttgaacag cggcgcgccg ctgtggttgg cgttgcccag
      721 caggggcggg ctggtgaaca gggccgtgtc gatgtggtat tcccagctgc ccgaaaacgc
      781 gcgcttgctg accagccgtg tcatgctgac ctcgccgtcg tcgctggcct gcgggccggt
      841 cacggccatc agcgcctcgc cggcttgcag gggctggctg ctgcccagca ctgcggcacc
      901 gacgccgctg ccggcgcgca gcggcagcaa gggccgcagc aggccgaaac cggtcgccag
      961 gtcataggcc acggcgcgtg ccggcaggat cttgttgtcc tgcgtgacga tttcaatctg
     1021 ctcggcctcc agcatcaggt agccgatggt caggatcagg ccgtccggcg cgatcatcac
     1081 cccggagccg cggcggtgct gacccagcgt ctcggccgac cgcgcctcct cgtccaccat
     1141 gacacgcacg ccgaccactg cggcgttggc ccgggccatc gccgcaatca tttcctgggc
     1201 gttcgggggc tgggccgagg ccgcttgggc gggcagggcc gccagcagga cagccgccac
     1261 gaagggatgg gtccaacgac gaagtgagtg catgacggac tcccgaagcc gcagaaaaaa
     1321 ctgcgcctcg agaatgcgcc gatttcaatg ccgtggcatt gcgcggggtc aagtcgcggt
     1381 tacccgcgcc tggcggcagg gcttaccggc tcaggcgcgc atgtacccag tgcgaggccg
     1441 ccagcaggcg gtgatcgtcg ccatgggcgc ccaccagttg caggcccacc ggcagcccgt
     1501 ggcggccccg ggcaaagggc aggtgcacgc agggcaggcc cagcagtgtc caggtgcggc
     1561 aaaacagcgg gtcgccggtg ccgtccagcc cggccggggc ctcgccgatg ctgctgggcg
     1621 ccagcaacac gtcgtgcggc ccgaagaggg cctgcgcctg gcgccgcgcc tgcgcggtca
     1681 cctgcaggtt gtgcgcatgg gcctcaccac cgatggccag gccggtggcg atcagtgcct
     1741 gcagcggcgc actgagctgg tcgcggtgcc gcaggcgctc atggctcagc gaacgcgcgg
     1801 tctcgaaagc ctgcacctct ttctgcacgg cgaccaggcc cgacagctcg ggcggcagcg
     1861 ccacaggcgc gccatggccg gccagcgcgg cttcggcccg ggcccaggcc tgctgcgtgt
     1921 cctcatcggc ctgcgcccat tcgggcgtgc ggcaaaagcc gatgcgcggc gcggctcctt
     1981 gcgcaaacgc ggcggggtcg gccagccgcg catcgccggt cagcaccgcg ccgagcagcg
     2041 ccacgtcgcg cacgctgcgc gcaaagccgc ccaccgtgtc cagcgtttcc gacaggcttt
     2101 tgacaccgcc gcgcggcacc cggttgtggc tgggcttgta gctgaccacg ccgcaaaaag
     2161 ccgccggtcg tatcagcgag ccagccgttt gggtgcccag tgccagcggc agcatggcgt
     2221 cggccacggc ggcggccgaa ccgctcgacg agccgcccgg cgtgtgatcc gggtggtgcg
     2281 ggttgcgcgt ggcgccgggg aacatgttgg ccaactcggt ggtcacggtt ttgccgagca
     2341 acaccgcgcc ggcttcacgg cacagcgcca cggccgccgc atcggccagc ggctgctggc
     2401 cggcataaat gggtgatccg taagcggtgg gcaggtcgaa ggtgtcgaac aagtccttca
     2461 cgccgaaggg caggccgtgc aacaggccac gcaccggccc ggcatcgagt tcacgggcca
     2521 ttgccagcgc ggcgtcggcg cccagctgca caaacgcctg cagctgcggc tcccgttcgg
     2581 caatccggtc caggcaggcg cggaccaggt ccacggcctg gagttcgcgg cgcgcgagca
     2641 gggcggcggc ttgccaggcg tcgagttgat gcaggggagt gctggaggaa gtcatggccg
     2701 ccagtatctg tgtttctgcg tttttgcgcg caagcgccca caaaaaaggc ccctccagaa
     2761 ctggaagggc ctgtatctgg tgctcggggc cggagtcgaa cccgcgtacg aagtgcgctt
     2821 cgtcaatctc gcccaaaggc gggccggttc gggagaacca ggcaacgaag ttcacaggag
     2881 gtggtgcccg gggccgtaat cgaaccggca cgccttgcgg cgggggattt tgagtcccct
     2941 gcgtctacca atttcaccac ccgggctgga agaagcaaag aggcaaatta tg
//
LOCUS       DAIDFG010000359         2986 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_359_length_2986_cov_15.462982, whole genome shotgun sequence.
ACCESSION   DAIDFG010000359 DAIDFG010000000
VERSION     DAIDFG010000359.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_359_length_2986_cov_15.462982"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          610 a          931 c          923 g          522 t
ORIGIN      
        1 caccctggtt ggccgtgtgg tcagcgacaa gcgcgcgaaa acggtgacgg tgctggtcga
       61 gcgtcgtgtc aagcacgagc tgtacggcaa gatcgtgtcc ctgtcgagca agtaccacgc
      121 ccatgacgaa aagggcgagt acaagacggg tgacctgatc gagatcaccg aaagccgccc
      181 gatttccaag accaagaact gggttgtgac caagttgctg gaaaaagctg ctgtggtctg
      241 aacctccccg gtttgaccgc aagtcatcaa aaaaggccca cgatgtgggc cttttttttt
      301 tccgctgtca gccgccctca ggtcgcccgc gatgcgcggc ggggctttcg ccggcccctg
      361 aacgcataag tcggaatcgt tgaaaatggt ggctgaccgt ttttactccc cctcattaac
      421 aaggagacac catgatcaaa gtaggcgaca cccttcccgc agccacgctg atggaatttt
      481 cggaagtcga aggcaatggc tgcagcatcg gccccaatcc cgtcgacgtg agcaaggcgg
      541 cggccgggaa aaccattgcg ctgtttggcc tgccgggcgc ttacacgccg acctgctctg
      601 ccaaacatgt gccgggttat gtcgagacat tcgacgagct caaggccgcc ggtgtggacg
      661 agatctggtg cgtgagtgtc aatgatgctt ttgtgatggg tgcctgggcg cgcgaccaga
      721 agaccggcgc caaggtccgc atgctggccg acggcagcgc cgacttcgcc aaggccaccg
      781 gcctgacact ggacctgact gcacgcggca tgggcctgcg cagcaaccgt tattcgatgc
      841 tggtcaagga cggcaaggtc gccgcgctga acgtagaagg ccccggcaag tttgaagtca
      901 gcgacgctgg caccctgctg gcacaagcca agggctgagc ccccgtccgg gctcgccgga
      961 ctgtttccgc agacaagccg cagatcgcag gacctgcggc ttttttgtgg gctgccgacg
     1021 gcgagaactt tcgctcagac gctatgaaaa aagtagctgt tagcgcccgt tgaataaggg
     1081 ctacagcctg atttgactac aaatccacct tcaggtagtg agcgcccgct atcgggttgt
     1141 ggtagtaggg cgggatgtcc ttgaagccca gatcttcgta cagcgccctt gccgcctcca
     1201 tgtcgttgag ggtgtcgagc aggaggctgt ggtaacccgc gatgcgcgcg gcatccagca
     1261 ccgcttcgac gagttgccgt cccagcccga agcggcgaaa gcccgggcgc acatacaggc
     1321 gtttcatctc gcaggcattg tggtagtcca ccgtctccag cgggcgcatc gcgcagcagc
     1381 cggcgatctc gccgtccacc agggccagca gcaggcagcc ctgcggcggc gcatagtcac
     1441 ccggcagacc ggccaactcg gcctcgaagt tctggaagca gaggtcgacg cccagctggt
     1501 cggcgtactc ctggaaaatc aggcgcgttg ccgccagatg ttccggcgtg gccggggtga
     1561 tgaactcgat ggagggagaa gaagcagaca tggcacggtg aagtccaacc cagtgtatcg
     1621 ggatttttcg gagcccccac cgggacttgc ccgggtctgc gctacaggcc gtgtcagaac
     1681 gcgggcgcgc ccaggctgct gatccagtag accccggccg cacaaaccag cagcaccccc
     1741 agggccagca gccgcgaggt cgtgtcgtca cgcgaggcgg gcgcgggtgt caccagtacc
     1801 ttgtcgccat gcagcatggc gcccaccaga ttgtgtttac gccacaggta gaacacgatg
     1861 gccccgatgt gcagcaccac cagagccagc aggatccacc tgccgatgtt cttgtgatag
     1921 tcggtcgcca ggccgacggt ggcgttggag acaaaacgcg tcagcggacc cgcgaaggcg
     1981 atttcgtcgt cgctgagcaa gccggtgccg acctgggcca gcaggaagcc cagcatggca
     2041 aaaaccgacc cggcgccgac ggggctgtgc ccgatgccgt gttcgggcct gccttttccc
     2101 ttgaggtagt tgatcacgct ggccggcgaa taaatgaagg caccaaaacg ggaccagcgt
     2161 ccgcccacca gcccccagac gatgcgaaac agcagcagcg tcagcaccga ataaccgaag
     2221 cggaagtgcc agaccatggc gttgccgcct atcgtgccgg tgaccgccag cccgatcacc
     2281 gcggcgacca gcgcccagtg aaaaagccgt gtgggcaggt cccagacgcg tattttgtgg
     2341 atgtgctggc tcatgcgtga ctcctcttgt tgtgtctgat ggtcgggccg tcatgccgcc
     2401 atggtatgtt tcatgcgtcg ggttcagtgc ccggccgctt ttccttggtg cttaccctag
     2461 tcctttagtt ccagcggaac tttgcaaccg ggataaggct cctttgactg gcagtctatc
     2521 gggaagcact gccccatcgc aagaacatct caaaaaccac gaggaaaaat aaatgaaagc
     2581 tttcgcctgt attgcagccg ctgccaccct gctcgccctg gccgctccgg cctctgccca
     2641 gtttgccaaa cccgaagacg ccatcaagta ccgccagagc gcgctgtccg tgatgggcac
     2701 gcatttcggc cgcctgggcg ccatggccaa tggccgtgtc ccgttcgacg ccaaagtcgc
     2761 cgcggacaat gccgagatcg tggctgccat ggccaaactc ccgtgggccg gcttcggtgc
     2821 gggcacggac aagggcggca acaccaaggc caagcccgaa atctggactg agcaggccaa
     2881 gttcaaggaa cacagcgaca agctgcaggc ggagtccacc aagcttgctg ccgccagcaa
     2941 gaccggcagc ctcgacagcc tgaaaacggc gtttgccgca accgcc
//
LOCUS       DAIDFG010000360         2972 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_360_length_2972_cov_17.660610, whole genome shotgun sequence.
ACCESSION   DAIDFG010000360 DAIDFG010000000
VERSION     DAIDFG010000360.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_360_length_2972_cov_17.660610"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          522 a          934 c         1001 g          515 t
ORIGIN      
        1 cctcggtgag tcgtttattg gttgcggctc atcgatgtat gaactttact gttgctgaca
       61 acaaagaaaa atagctatta aatcaattgg taattgctgt ttatggaata ataagggctt
      121 gatttactaa gtacttaccc catggaccga cttttatcca tgcgtgcctt ccagcaagtc
      181 gtggacgacg gcgggtttgc cgccgccgcc agaacgctgg acatgtcgcc ggccgtgatg
      241 acccgcctga tcacggacct ggagcagcac ctgggggccc gtctgctgca gcgcaccacc
      301 cgcaagctgt cgttgaccga ggcaaggcag tcctacctgc agcgggtgcg gcacatcctc
      361 aacgacgtgg accaggccga ggcttccacg cgccagcaaa ccgacgaact ggccggcctg
      421 ctgcgtattc acacgcagcc cgtgatggcg gtgcacatca ttgcgcctct ggtggccgag
      481 tttcgccgcc gttatcccaa ggtggtgctg gacatcacgg tggactcgcc gctggtgccg
      541 ccgatcgagg attacgacct gacaattctg ggcgccggcc ggctgtatga cgccaacgtg
      601 gtggcgcgtc cggtgattgt ttcagagggc ctcctgtgtg cttcaccgcg ctacctgcgc
      661 agtgcaggca cgccgcggca gccggacgac ctgaagaacc acgccctgct caggctcagc
      721 caggcgggta tccgccagcg cgagtggcaa ttgatcaacc ccgaggaaga tgaccgggag
      781 gtggccgtgg aggtcaaacc cgtgatgatg gccaaccaca gcgacaccct gctgcgcgcc
      841 acgctggacg gcactggcat cagcgcgcag ccgctggacc tggtggccaa ttacctgcgc
      901 gacgggcagc tgcggcgcgt gctggcgccc tggatcaccg gccggttcac gctgtacgcg
      961 gccctgccca gccgcaaata cgtgccggcg cgggcccggg tgttcatgga gttcctgatt
     1021 gagcagaccc ggctgaaagc cgccgaggcc gagcgcacct ggccctccag gggcgaagac
     1081 aaggcgggtg atcccgccgc ctaaccaggc gcgccgggcg tggcaaagtg gttttgccgc
     1141 caggcttcaa acaccgtgac ggccacggcg ttggacaggt tcaggctgcg ctgaccgagc
     1201 tgcatgggca gtttcagacg ttgcgccggc gcaaacagct cacgcacctc gggggccaga
     1261 ccccgggtct ccgcgccaaa caccagccaa tcgcccggtg cgaaaacggt gtcgtgcacc
     1321 agtcttgtac cgcgagtcgt gagggcaaac attctttgcg ggtcgggctg ctcggtgacc
     1381 agaaaagcgg gccagtcggc atggcgtttg agctcggcgt actcgtggta gtccagcccg
     1441 gcacggcgca tgtgtttgtc gtccatcgaa aaccccagcg gctccaccag atgcaggcgg
     1501 cagccggtgt tggccgccag acggatcacg ttgccggtgt tgggggggat ttcgggctcg
     1561 accagaacga tattgaacat cgcctgattg tcgcgtgtcg ctgccctagc tggcttcggt
     1621 acgagcgact accaagccgc tgacgccgac ggcgccagcc tcgcgcaggg cccgtgcggc
     1681 cgcaaacagg gatgcgccgc tggtcatcac gtcatcgacc agcagtacgt tgcggcctgc
     1741 cagttcgcct gcgcgcaggg gatcgacgac aaatgcaccc ctgacgttct gcagccgcgc
     1801 ttcgcgcttg agttgggact ggggtggcgt gttcctgata cgcagcagca gccgggcgtc
     1861 ggggcgcgcg gacgtgccgc tttggccggc cagtgccttg gccagttccc aggactggtt
     1921 gaaaccgcgt gtctgcaggc gttcggccga caggggcatg ggaagcagcc agtcaccggg
     1981 ctgcagggct gcgagccgcc cggggacgcc gggtgtgcgc agaagcaaac cggcgaacac
     2041 cgcggcccac gccgggtcct ggccgaattt gtagacggcc atcagtttcg accagggata
     2101 ggcataggtc acggcggcaa aacacgcgtc cagcggctgc ggctgcctta cgcaggtggc
     2161 gcattgggcc tggccgctga ccaggctgat cgcgcaggtg ctgcaacgcg ccgccggctg
     2221 gccgaaacgc gcgacgcagg ccgggcagac acggcgcgct ggccagctgt ggcacaccgc
     2281 gcactggctg gccagatttt gcagtgtcaa gaaagccggt gtggtcaggc gcaacgacat
     2341 cgtgtcaata tactcgtcgc tctgcctgcc tgattttctc catgtccctc ttctgatgcc
     2401 caacgaccgc ccacccactc tggatccgat agctgttgcc cgctggcaac gcgtggcgcc
     2461 ggcggcatcg ccctggctgc atgaggaagt ggcgcagcgc atgctggacc ggttgcagtg
     2521 gatcaggctc aagtcgcagg cctgggccca ctggggggcg gtgcgcgggg gcctgcaggc
     2581 gcatcggcac ctcgccgacc gttatccgga tgccgaatgt tttgtggtcg aggcccagca
     2641 ggagtttgtg caggaagcta tcaaaaaaat agcaaaaccg tggtggagtc cgaagcgctg
     2701 gacggcgcca gccatgcatg tcgaggcccc gccgccagcc ggggtggaca tgctctgggc
     2761 caacatggcg ctgcatgagg ccgccgatcc gcaggacttg ctggcgcagt ggcatcgggc
     2821 actcaaggtc gggggctttc tgatgttttc ctgcctgggt ccggacagcg cacgcgagtt
     2881 gcgcgaggtc taccgggcgc tgggctggcc gccggccggc cacgaactga cggacatgca
     2941 cgactggggc gacatgctgg tgcagaccgg tt
//
LOCUS       DAIDFG010000361         2940 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_361_length_2940_cov_21.170191, whole genome shotgun sequence.
ACCESSION   DAIDFG010000361 DAIDFG010000000
VERSION     DAIDFG010000361.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_361_length_2940_cov_21.170191"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          559 a          901 c          871 g          609 t
ORIGIN      
        1 aaggtgcagc tgcgcgagct gatcgatcag atccgcaagg acaaccgcac catcttgctg
       61 atcgagcacg acgtcaagct cgtcatgggc ctctgcgacc gcgtcaccgt cctcgattac
      121 ggcaagcaga ttgccgaagg cccgccttcc gaggtgcaga aaaatgaaaa agtgatcacc
      181 gcctatctgg gcacaggtca ttgaaggaaa tcaagaacat ggccaccaca agtcatacca
      241 ccctgctcaa agtcaccggc ctcaaagtgg cctatggcgg catccaggcg gtcaagggcg
      301 tcgatttcga agtcaaggag ggtgaactgg tcacgctgat cggctccaac ggcgctggca
      361 agaccaccac catgaaagcc atcaccggca cgctgccgct gctgggcggc gacatcgaat
      421 acctgggcaa aagcatcaag ggccagggcg cctgggacct ggtcaaggaa ggcctggcca
      481 tggtgcccga aggccgcggc gtgttcaccc gcatgaccat caccgaaaac ctgcagatgg
      541 gcgcctacat ccgcaacgac aagcccgaga tcgccgccga catggagaag atgtttgcgc
      601 tgtttccacg cctgcgcgaa cgcaaggacc agctcgccgg caccatgtcc ggcggcgagc
      661 agcagatgct ggccatgggg cgtgcgctca tgagccgtcc ccgcgtcttg ctgctcgacg
      721 agccgtccat gggcctgtcc cccatcatgg tcgacaagat tttcgaagtc gtcagggacg
      781 tgtcggcgca gggcgtcacc gtgctgctgg tcgaacagaa cgccagccgg gccctgggca
      841 ttgccgaccg cggttatgtc atggagtcgg gcctggtcac gatgaacggc gaggcgaagg
      901 aactgctcaa cgatccgcgg gtgagagcgg cttatctggg ggagtgattc ttcaaggagt
      961 tttggcttca agaccttgca cgacgggcgc cagcagctat tgaatcagta gcaaaatcag
     1021 agccttcccg ggcctgattt ttgtatgcga tacagcttta ttttcccgcc gcgttcgact
     1081 tttcaatccc ggtcgccacc gcctgggcca gactgttcga caagccactg tcacccgtca
     1141 ccttgccgag cgccgcaccg gtcaccagtt gaatcaggcg ggtcgatggg tcggtattgg
     1201 ccgatgtgcc ggccgaacct tccggggcgg tggcgctttt catcaggccg ctgaccaggt
     1261 ccatggcaaa acggttgcct gaacttccgc caccaccgcc tgaagagccc gccatcgtca
     1321 aggctacctg gcccagaatc gcaaaagccg tgcgccaggc tttttcactt tcctgctgct
     1381 tgaaatcacc cacggcaatc tgcaggtcgc ggtggtactg ctgctgcagc agggccatgt
     1441 agtcgggccg cgccggcatg aacatcacca cgccaccgcc gactactgcc gaattgctga
     1501 caaacaggcc ggtttccttg ctgttgtctt ccagatccac cttgtcaccg taaaagatga
     1561 tgtggcccat ggtgatgcgt cctgcctcca tccccttggc cggcatgtag tcgaagcgac
     1621 cgcggaattc gatgccgttg cccagggcat acacaccctc gtagggcacc gccagccgcg
     1681 cccgcatgaa ggcgtggtcc aggcgtccga agctgccggt gaacaccggc acgatgttca
     1741 tggcgccaaa accgcgtatc tggctgccgc ggtccatcag gccgtccgcg ccgtagaact
     1801 gctcgtactt cttgcccttg gtcagcggat ggctgaccag gtaacgctgg ccgggaatgg
     1861 tcttgctgtt ttgccagacg ccttcgagca tctggttctg gtacatgtcg tgcaccgttc
     1921 cctggccgtt gcggcagtcg ccctgcacac agcggtaaaa gtcggcattg gagatgtcca
     1981 gcgcatggac ggccggcgcc aatgcagcca ccagcgtggc cagcgccatg gcgcggcgtg
     2041 aagaggaagc ccgcatcccc tcagtccttg ggacaggtct gggctttggt caacacgcct
     2101 gcaataaagc agttgcgggt ttccagcatt tccacgccat tgacagtcac cagccgggct
     2161 ttctccatgc cgggcatctg gtccagccgc atgcccgatg ccttcaggta gttctcctgc
     2221 acctgcactt gctcgccgtg gtacgtgccg tttttcatgg gcgtctcgct ggtcatgaaa
     2281 aacttgtcgt tcttgtacat gaccattttg ttgacaggcc cgctcatgcc ggacgtgaaa
     2341 tggctgtcca gctgctgctg gaagctggtg ttgacaccaa acagcccgcc ggtgattttg
     2401 ggcgccatgc tcacggtgaa agtagccatt tgcccggagc tgaaattcat gtaatacagc
     2461 gaaacctgtg gctcgggcgc atccggaatt tttgttttgg ccatggtctg ggccatttgc
     2521 gcatcgtcgt acagggtctc gttacgcatg gacaactggt tggcgtggta gcggtctccg
     2581 tcctggacct tgccgtcctt gaaagtcagg aaatagcggc tggcctgctg cgtccccatc
     2641 ttcatggtca tcttcacctt cctggtgccg aggtccatgt tgttgacgtt ttccatcagg
     2701 gtgtcgaact gcaccaggaa atcggtgtcg ccctcgagct tgccgtcctt gcaggccgcc
     2761 ttgagcggcg tgatgcggat gttgctgtac tgcatgggct tggcggcggt gtccgggtcc
     2821 atgcccatgt cgcgcagctg ttttcgcgac atcagccgga tcttctcggc ctcttccgga
     2881 atcgccatga aaagtccgag cggctggtac aggtcacgct ggggaacttc acaaggcatg
//
LOCUS       DAIDFG010000362         2939 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_362_length_2939_cov_12.598474, whole genome shotgun sequence.
ACCESSION   DAIDFG010000362 DAIDFG010000000
VERSION     DAIDFG010000362.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_362_length_2939_cov_12.598474"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          464 a          867 c         1025 g          583 t
ORIGIN      
        1 gcgagcacct tcatcattgg cggcttcgtc attttgtggg ccgagcggcg gcaaaaaatg
       61 aatccggcca cggcacgcat ccacgaggtc gaagccatga ccatgatgga tgcgctcaag
      121 gtgggcctgg tgcagtgcct ggccatggtg cccggcacca gccgcagcgg cgccaccatc
      181 atcggcggca tgctgctggg cctgtcacgc aaggcggcga cggatttttc gttttacctg
      241 gcgattccga cgctgatcgg cgccggtgcc tacagcctgt acaaggaccg cgccctgctg
      301 tcagcggcgg acctgccgct gtttggcgtg gggctgctgt ttgcttttgt cagcgcctgg
      361 ctgtgcattc gctggctgct gcgttacatc gccacccaca gttttgtgcc ctttgcctgg
      421 taccgcatcg tcttcggtgt ggtggtgctg gtgacggcgc agatgggctg ggtcacctgg
      481 gcggcatgaa gggcgtgcgc ggctgagcgt tcacgccgcg ccgctcaatc catcaggttg
      541 cgcagggccg cttcgatggc cgccgccgtg gccacgggtt tttccatcgg gaacaggtgg
      601 ctcccgtcga gcatcatgat gcggcctttg gtgacctttt cggtcatggc catgcccacc
      661 tgtttcatct cggccgactg gcgcccgccg atgaaggcca ccgggcactt gagcggatgg
      721 cgcttgagca ggccctcgag gttgtcgggc agggtgttgt agatggcggt ttccacatcg
      781 cggtcaaaac tcagcaggcg ctggccgccg gcatcgtgtg tgccgtgttc gatgtagtcc
      841 agcagcacct gttcatccca gcgtgcaaag gcttttttgg tgcggaaatg ggccagcacc
      901 tcggcctggc ccagccactc gtgccggcgc ttgcggctga tggcgccggg tgaaatcgag
      961 ccaaccagct gcgcacgttt ggccacgctc agcgcggcgg cacgccagcc gcccaggatg
     1021 ggcgagtcga tcagcagcac accacgcacc ggcaggccgc cgagcacggg attgcgcgcg
     1081 gcgcacatca ggctcaggaa gccgcccagc gagtggccga ccagaaaggc cgcctggccg
     1141 gttttttcga cctgctcgct ggtgaagtcg gcgagctgct gcaccaggtt gggccagttg
     1201 ctggtgaccg ggtaacgcgg gtcgtggccg aatttttcaa tcgccttgac ctggaagccg
     1261 cgcgcgcgca gcccctggaa caacacgccg taggtgcttg cgggaaagct gttgccgtgg
     1321 gagaaaatga tgaggggttt ggtggaggcg ggcacgtcag atcagctttt cttcgggcga
     1381 ggtgattttt ttcagcggct ggttgcgtgt cagcggcgtc aacagcggca cctgggtttc
     1441 gccgccggtc cacatcgggt cctcgatgct gtcgaaaact tcgcgcagct ttttgcccca
     1501 gttgtcgtgc agcatgcgga agtaggggtt ctcgtggtcg atgcagacca ccttgtcgct
     1561 ttgcagcgag ttgacttcat aaaccaccat gtccagcggc agaccgacgg acaggttgga
     1621 cttgagggtg gagtccatcg agaccagcgc gcatttggcg gcttcgtcca gcggtgtttc
     1681 gggggtgatc acgcggtcga gcaccggttt gccgtatttg gattcgccga cctggaagta
     1741 gggcgtctcc ggcgtggctt cgatgaagtt gccggccgaa tagagctgga acaggcgcat
     1801 gccttcaccg cggatctggc cgccgaagat cagcgagaca ttgaactcga caccggcatg
     1861 tttgaggtcg tcggcatcgc gctcgtgcac gcggcggatg gccgcaccca gcacacgcgc
     1921 cgcgtcgaac atgcttttgg cgttccagat ggtgatgggc gggccgcctt ccgggtcctt
     1981 gagctgctcg atctgcagga tctcgcgcac tgactgcgag atcgacaggt tgccggccga
     2041 cagcagcacc atgaaacggt cttccggctt ttcgtaaatg atcatcttgc ggaaggtgct
     2101 gatctggtcc aacccggcgt tggtgcggga gtcggaaaga aacaccaggc cggcgttgag
     2161 tttgatggcg acgcagtagg tcatgggggg caatcgttgg gggtgggacg gctcaggcag
     2221 ccgctatttt tttcagttgg tacagcgcgt ccagcgcctc gcgcgggctc agcgcatcag
     2281 ggtcgatgcc gtgcagggct ttttccacgg cactgggttc ggcggcgggc tgctcgggcg
     2341 gcgcggcaaa caggtcgacc tgggcgcggc tggcggtttg ctgggtttcc agcgcatcga
     2401 gcgcatggcg cgcgtggttg accacggccg ccggcacacc ggcgagtttg gccaccgcga
     2461 taccgtaact cttgctggcc ggaccgggct cgatgtgatg cagaaaaacg atgctggcac
     2521 cggactcgac cgcgctgacg tgcacattga ccgccgcatg gtggcgcgcc gggaattcgg
     2581 tcagttcgaa ataatgggtt gcaaacagcg tgaaggcctg ggtcttgttg tgcaggtggg
     2641 cggcaatgcc gccggccagc gccaggccgt cgaaggtgga tgtgccgcgg ccgatctcgt
     2701 ccatcagcac cagcgagtgc ggcgtggcgg cgtgcaggat ctgcgccgcc tcggtcatct
     2761 ccagcatgaa ggtggactgc gcgttggcca cgtcgtcggc cgcgccgatg cgggtgtgga
     2821 tggcgtcgat cggccccagg cggcaggcgc tggccggcac gaaggagccc acgctggcca
     2881 gcagcacgat gatggccacc tggcgcatgt aggtggactt gccgcccatg ttggggccg
//
LOCUS       DAIDFG010000363         2922 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_363_length_2922_cov_18.947332, whole genome shotgun sequence.
ACCESSION   DAIDFG010000363 DAIDFG010000000
VERSION     DAIDFG010000363.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_363_length_2922_cov_18.947332"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          518 a          912 c          873 g          619 t
ORIGIN      
        1 gcatgggcat catgacggtg cgcgacgtga aagacctcga cgagctcagc cgcaagacca
       61 aaaacaagat gggtaccacc aaggacggcc agccgctgtc cgaggtcatc atccaggagg
      121 gggtgcagac ccacgagcgc atcaacgacg cggtggcaga gcccgtcgtc tacatgatgg
      181 accgctacgt ggtgggcggc ttttaccgcg tgcacgccga ccgcggcatc gacgagaacc
      241 tcaatgcccc cggcgccagc ttcgtgccgc tggcttttgc cgacagcggc cggttgccgc
      301 aaccgggcgt caaacccggc tccagcgggc ccaaccgctt ctacatgtac ggcgtgattg
      361 gccggctggc catgctggcc gccagttacg aactcgaagc caccgatccg gacgccgaag
      421 tctacgacta gaggcttgtt cacccctgtc gttctcactt cgctgcgctt cgtgtcgtgc
      481 cttttcccct tccaggggac gccgcgaggc atccggcgaa gccggcctgc ccgcggctgc
      541 tgatgacgcc ggggaaggcg ttgcgtacct tggacgctca aaactgttca gttgcggatc
      601 ttttggcctc cagcccttga tgggcgggcg ccggaagcta tcaaaaacag agcgaatttt
      661 gcctccttcc aggggaacaa aaaaaacggc cgcttcgcgc cgctgttaca agttgttgca
      721 gtgcaacaaa acgccggatt ctcgcgatta ggggtctgcg ctgacttgcc aagtgcgggg
      781 cgaggcgcaa aatagcgttc ctcatggcaa cccttcccgc cgctggcacc tgactgatcc
      841 agcccaagcg ggaatttttt tgtgtccagt aaaaaatcat cacttgcggc gctgaccctg
      901 ggcgccgtcg gcgtcgtcta cggcgacatc ggaaccagcg tcctgtatgc catcaaggaa
      961 gtcttcggct ccgggcacgt cgcattcacc cctgaaaacg tttacggcat cctgtccatc
     1021 ttcttctgga cgctgaccgt cattgtctcg atcaagtacg tgctgctggt gctgcgtgcc
     1081 gacaacaacg gcgagggggg tcttgtcgcc atgctggcgc tggcctcgca ggcggtcaag
     1141 gacaagccgg aactgcgccg tattctcctg atcatcggca ttttcggcac ctcgctgttt
     1201 tatggtgacg gcgtgatcac gcccgccatc tcggtcctgt ccgcggtcga gggtctggag
     1261 gtcatttcgc cggccttcga aaagtacgtg atcccgacca ccctgttgat cctgtttgcg
     1321 ctctttgccg tgcaaaagcg cggtaccgcc ggcatcggca agtttttcgg ccccatcacc
     1381 ctggtctggt ttgccgtgct ggcgctgctg ggtgcggcac agatcatcac caaccccatc
     1441 attctcaagg cactgagccc gcattacgcc gtgatgttca tgtggttcaa cccggggacg
     1501 accttcatca ttctcggcgc ggtggtgctg tgcgtgacgg gggccgaggc cctgtatgcc
     1561 gacctcggcc acttcggcaa gaagccgatt cgcctggcct ggtttgccgt ggtcatgccc
     1621 gccctgaccc tgaattattt cggccagggc gcgctgctgc tgagcaaccc ggaagccgtg
     1681 aagaacccct tttacctgat ggccccggat tgggcgctgt tgccgctggt gctgctggcc
     1741 accatggcga cggtgatcgc ctcgcaggcg ctgatcacgg gggccttcag cgtcaccaaa
     1801 caggtgatcc agctgggtta cctgccgcgc ctgaacatcg agcacaccag cgtgcgcgac
     1861 accgggcaga tctacatacc cctggtgaac tggggtctgt ttgtcaccat cgtgttggcc
     1921 gtggtgatgt tccgctcgtc gagcaatctg gcggccgcct acggcatcgc cgtctgcacc
     1981 gatatgctga tcacgaccat cctgacgttc tatgtgatcc gttatggctg gaaatacccc
     2041 ttgtggctct gcctgtccgc caccggtgtc ttttttgtcg tggatttcgc cttcttcgcc
     2101 tccaacctga tgaagctgtt tgccggtggc tggtttccgc tggtcatcgg cgggatcatc
     2161 ttcacgctga tgatgacctg gaagcagggg cgggcattgc tcaacgagaa actgcgggcc
     2221 gatgcgatcg acctgcccgg cttcctggag gccgtgttca tcagcccgcc ggcccgcgtg
     2281 gaaggcacgg cggtgttcct gaccgccgaa ccgggtgccg tgcccaatgc gctgctgcac
     2341 aacctcaagc acaacaaggt gttgcacgag cagaacctgt ttgtcacggt gcgcaaccat
     2401 gaggtgccgt ggattggcat ggacaagcgg ctggcgatcg agtcgctggg ccacaactgc
     2461 tggcaggtgg tgatccacta cggtttcaag aatgacccgg acctgcccaa ggccctcgaa
     2521 cagatgcgca cgcgcggctg cgaactggaa gtcatgacca ccagctactt cctctcgcgt
     2581 gacgtggtgg tgcccaccat aggcagcggc atggcgccgt ggcgcgagaa gctgtttgcg
     2641 cagatgcacc acaacgccag cggcgcggcg gacttcctca acctgccgaa taactcggtg
     2701 gtggaactgg gtagcaagat cgagatttga tcttggtgtt ttgtggctgc agccctttcc
     2761 ccgtgagcgc aggcagctat tgaatttata gctaaacctt cctcgtactt gtcatcccgg
     2821 acttgatccg ggaccatgtt tgctaactcg cgctttgaga tgtgccaaca agccgggtct
     2881 cgccccggcg ggcgactcac tttctcttgc gtcgccaaga ga
//
LOCUS       DAIDFG010000364         2915 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_364_length_2915_cov_15.886014, whole genome shotgun sequence.
ACCESSION   DAIDFG010000364 DAIDFG010000000
VERSION     DAIDFG010000364.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_364_length_2915_cov_15.886014"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          525 a          930 c          932 g          528 t
ORIGIN      
        1 gggcgctgcg cctgcggccc ggcgaagccg gttccgcggc ccctgcttga aaagagatgc
       61 cttccgcagt cgcttgttgt taccccgttc gtcctgagcc tgtcgaagga tgccccgcaa
      121 gccgttaaaa tagcgattca cccgcgtgct gtcccggcgc gctggtgttt ttattttccg
      181 gggccatgta gaggaccacc atgtacaaaa acctcgctgc cgcgctcgcg gccgcctgtt
      241 ttatttcacc cgttttttcc caacccgctg cgcccgccaa agagcctttg aaaatcggct
      301 tcgtttatgt ggcgccgctg accgatgccg gctgggtgcg ccagcatgat gacgcccgca
      361 aggccgttga gacggcgctg ggcaaccggg tcaaaaccac ctatgttgaa aacgttcccg
      421 agggggccga tgccgaacgc gtgatccgcg acctggccag ccagggccac cggctcatct
      481 tcacgcccag ctttggctac atggagccga cgctgaaggt ggcgaaggac tttcctgacg
      541 tgaaattcga gtccatcacc ggctacaaga ccgcccccaa cgttgcggtg gccaatgcac
      601 gttattacga aggccgctac ctggccggca ttgctgccgg ccgtctcgca tccagcgccg
      661 gctatgtcgc gggttttgcc attcccgagg tgatccaggg catcaacgcc ttcacgctgg
      721 gcatgcgttc ggtcaatccg aaagccgaag tgaaggtggt ctggctgggc gcctggtttg
      781 acccgccgcg cgaacgcgaa gcggcgatga cactgttcaa ccagggtgtg gaagtgatcg
      841 cttttcacac cggctccact gcggtgatga gcgcggccca ggagcgcggc aagctggcca
      901 ttgcctacca ttcggacatg cgcaaggtgg cgcccgacgc acaggtcctg gcagtcacgc
      961 accagtgggg cgactactac acccgccgtg cgcgggcggt gctcgacggc agctggaaaa
     1021 gcggcgcggt gtggggcggc gtgaaggagg gcatgatccg cgtcgacagc ttcggcccca
     1081 aggtgccgaa aaaagtggtt gaggaggtgc tcgcgcggca gaaggacatc gctgccggca
     1141 agctgcaccc tttccgggcg cgtacgccgc tgctggacaa cgagggccgc gaggtgctgg
     1201 cggccggcaa gacgctgtcg gacgagcaga tcctgggcat gaacttcctg gtgcagggcg
     1261 tccagggcaa gctggccaaa tagaccttat gcgggccggc atatcggcgg accccgccgc
     1321 tgcggtaagc tttcctggat gaaagcctgg cgcgcctccc ttctttattt tcccgcaccg
     1381 gcgcaggccc agctggaaac cgacggcctg ctggtggtcg gtccggacgc cgttggccgt
     1441 caggtcgtgt tggccgtggg cagctacagc gaactggcgt cgcgttttcc cggacttgac
     1501 gtcacccacc tgccgggccg catcattgcg cccggttttg tggacatgca catccactac
     1561 ccgcagctcg acgtgatcgg ctcacccgct gaaggcctgt tgccgtggct tgagaactac
     1621 acctttccgc atgaaagccg gttttcggat gccgcgcaca acagcgaagc ggccaatttt
     1681 tttgtggatg aactgctgcg ccacggcgtg acgacggcgc tggcctttgc cacctcgcat
     1741 cccgagtcgg tggatgccct gcttggcgaa gcgcagcgcc gcagcttgcg aatgatcgcc
     1801 ggcaaggtgt tgcaggacca gaactcgccg gatggcgtgc gcgacgctac ccaacaaagc
     1861 ctgcttgaca ccgaggcgct gatcacgcgc tggcacggcg tggaccggct cggttacgcg
     1921 atcacgccgc gttttgtgcc cagttgcagc gaagcgcagc tgcgcggcgc cggcgagctg
     1981 gccgcccgct acgccgatgt ctggatccag tcgcatgtgg ccgagaacaa ggatgaaatt
     2041 gcctgggtgc gcgaactgta cccgcaggcg cgcagctacc tcagcgtgta cgaacagttt
     2101 ggcctgttgc gtccgcgcgc tgtgtacgcc cactgcatcc acatcgacga cgaggaccgc
     2161 gcgctgctgc gagccaccgg cacggccgcg gccgtcagcc cgaccagcaa cctgtttctc
     2221 ggcagcggct tttttgacta cgccgccgcc gaccgcgctg gttttcccta tggcctggcg
     2281 agcgacgtgg gcggcggcac cagtttcagc ccctttcaca ccatgctcgc tgcgtattac
     2341 gtgggccggg aagggcagac caagcccggc gtgtcactca agccgcagca gttgtggtgg
     2401 caacacaccg gcggcgcggc gcgggcgctg ggcctggagg gtgtggtggg caacctgctg
     2461 cccggctgcg aggccgactt tctggtgctc aacccgcaag ctacgccgct gctggcgcgc
     2521 aagacggcgc aggccgccag tctggacgaa ctgctgtttg cgctgatcgt gctgggggac
     2581 gaccgcgtgg tcgagcaaac cgtgatttcg caggcgcaag cggcctgagc ctcagttttt
     2641 tcgacgtttt acaagaaaaa tcagcctcca gcccatacgg gacgggcgca agaagctatc
     2701 agatttatag taaacctgat gccgacctgt ccctgccgca gcttgcctac aattggcggc
     2761 aacgcaagga accttccgaa tgagcatcaa aagcgacaaa tggatacgcc gcatggccga
     2821 gaccaccggc atgatcgaac ctttcgagcc gggccagatc cgcgaaagcg aaggcaagaa
     2881 gatcatcagc tacggcacca gcagctacgg ctacg
//
LOCUS       DAIDFG010000365         2885 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_365_length_2885_cov_18.168198, whole genome shotgun sequence.
ACCESSION   DAIDFG010000365 DAIDFG010000000
VERSION     DAIDFG010000365.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_365_length_2885_cov_18.168198"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          548 a          891 c          916 g          530 t
ORIGIN      
        1 gcactcgagg cgcttggcaa tcgcccgcgc ccgcaccacg cccccgacgt cgggcgacac
       61 caccaccagg ttcgggtggt cacgcttcca gatgtcgccc agcaggatcg ggctggaata
      121 gatgttgtcg accggaatgt cgaaaaagcc ctgaatctgg tcggagtgca ggtccatcgt
      181 caacacccgg tcgaccccca cgccctgcag catgttggcc accaccttgg cgctgatggg
      241 cacgcgtgcc gaacgcgggc gccggtcttg ccgggcgtag ccgaagtagg ggatcaccgc
      301 ggaaatgcgc tcggccgagg cgcgcttgag cgcatcgacc atgatcagca gttccatcag
      361 gttttcattg gtcggcgcgc aggtggactg caccacaaac acgtcacgcg cgcgcacatt
      421 ctgctggatc tcgaccgtca cttctccgtc ggagaaacgt cccacatggg cggcccccag
      481 cgaaatgccc agatgctggg cgatctcgga tgccatgctc ggattggcat tgcccgtgaa
      541 aagcatgaag tcggagtggt gcggttgcat gtgtggccca gcgggtgtcc aggaagaaaa
      601 cgttgctgaa tggataaaag caataagcgc actgaaaaca cggacgcccc gtgtccgttc
      661 cgtgtcaatg cggcttctgg ccatttcctg cccggccaat tcagcggctg aattgcgcag
      721 gcagagcctt caccggagac actcaacagt cagcgagcac ggcccccgtt gtcgggtggc
      781 ctaaaaacac gggccccaca tgggacccga aaattttggc aggggtggaa ggattcgaac
      841 ctgcgaatgc cggaatcaaa atccggtgcc ttaaccaact tggcgacacc cctacacagg
      901 tagattgtcg gacctgaaaa ctgtcaaaga cagatgtcag gggaccggca accaaccctt
      961 taatcgttgt ttctggaaac acattccatg gcccttatct taccaaccgg ccagaggatg
     1021 tgcatccagg ttgctgcact tgcggacctg ccagtcgccc ggggcgaccg cgagttccat
     1081 ctcgtgcggc atatgcgcaa atacagcact tcccgagcca gtcatgcggc cttgcaaatg
     1141 ctgcatggac agccaatcga gggattggcc aacctgcgga cacaaggtct gcgcgactgg
     1201 ctgcaagtcg ttatggccga attcgtaaac agagccattt gcgtttgcag caaagcctcg
     1261 gattgtagca gtttctgtat cgcgtttgag cccaggcgag cggaaaatgg cgtcggtcgg
     1321 cagcccggca gcgggcttga ccacgacaaa gcgggcagca ggcagggcca gtggcgtgat
     1381 tttttcaccg acaccttcca cccaggcgtg gccgccgctg aggaaaaacg gcacgtcggc
     1441 accgaggccg agggcgatgg cctgcagttg ctccgttggc agcctgacgc cccacaggcg
     1501 ttgcaggctc aagaggcaac tggccgcatc ggacgagccg ccgcccatgc cggcctgcga
     1561 cggaatgcgc ttttccagcc cgatgtgcac acccagtggg gtgccggtcg cgctttgcag
     1621 cgcccgcgcg gcacgcacca ccaggtcttc ggccggcagc gcctcgctgg cctcgctgcc
     1681 aaggtcggtg cgcgtgatct ggccgtcctg gcgcagttcg aaatgcaggg tgtcgcacca
     1741 gtcgatcagc atgaacaccg actgcagcag gtggtagccg tcgggacggc gcccggtgat
     1801 gtgcaggaac aggttgagtt tggccggcgc cgcgacatcg tagatcgctt tcagcggcgg
     1861 ggccgaagca gggcgtgaca gtccgacttg catcagggtg ccgccagcac gatgcgcagg
     1921 tcggagcgcg gttgcggcat cgtccggctg gccgaaatcc ggccctcggc ctgttgcgaa
     1981 agatcggcat tccagccggc cgcggcggtg tttttgccgt ccagccagtc aaacagcgcc
     2041 ggcagcggca cggctgcgcc ggtggctttt tcaatcagtt cgtcgacggt ggcaaagcgc
     2101 tggatctggt tgccggaatc gagcacggcc tcctgcggcg accagcgcag cacggccagg
     2161 ctgttgccca acgggctgtt cagcgtcagc tcgccctgcg tggggttgcc cttgagttcg
     2221 aagccggcaa aaaaggcctg gggcggctcg ctttgcacct gcaggctgat gcggcctgcc
     2281 cagaatgatt ttttttcatc attttggcct gtagcccttg gtggacttgc gcaaccagct
     2341 atgaaaaatg tagcaaataa caggctcgcc agcagggcgg ccgaccggcg cagcggggcg
     2401 tcaaagacgg tcatggcggt ttacagcctg acccgcaggc gcttgagggt acccagcagc
     2461 gtctcgttct cggcgttgag cagcatgccc tccttccaga tggccagggc cctgtcgcgc
     2521 tggcccagac tccacaggac ctccccgaaa tgcgcggcga tctcggcatc gggcttggcc
     2581 ttgtaggcgg cttcaaaaat gctcaaggct tcggccttgt tgcccaggcg gaactccacc
     2641 cagcccaggc tgtccttgat gaaggggtcc tcgggtgcgt actccagtgc cttgagaatc
     2701 agctgtttgg cctcgggcag gcgcacgttg cgatcggcca gcgagtaacc cagcgcgttg
     2761 taggcgtggt ggtagtcggg cttgcgctcc accacctggc gcaacaggcg ttccatttcc
     2821 ggcagcgcat cgagcttctc ggccatcatg gcctggtcgt agagaagctc gggttcgtcc
     2881 gggaa
//
LOCUS       DAIDFG010000366         2885 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_366_length_2885_cov_14.689753, whole genome shotgun sequence.
ACCESSION   DAIDFG010000366 DAIDFG010000000
VERSION     DAIDFG010000366.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_366_length_2885_cov_14.689753"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          553 a         1002 c          822 g          508 t
ORIGIN      
        1 accctggctg gcggccgctg cgcccggcct gctctacacc gccatttcgc tgggtgcctt
       61 cggctggctg gtcctgagac gctgatggcc cggcaaggca ttgtcctgtt tgcgcacggc
      121 tcgcgcgacc ccctctggcg cctgcccatc gaggcggtgg ccgaggcgat ccgtgcgcgc
      181 gaccccgacg ccctggtctg ctgcgcctac ctcgagttgt gcacgccgga cctggcccag
      241 gccgctaccg atttgatagc tgccggcgca agcacaataa aggttttccc gctttttctc
      301 ggtgtaggaa aacacgcgcg ggaagacctg ccgctgctgc tggcacaagt tcgcgcccag
      361 cacccggacc tgccggtgac cctgatgcag acggctggcg agcaccccca gctgacggca
      421 ctgatggccg atatagccct gtcctgaacc gggggcgtga gctttttttt taagcgctgc
      481 ccgagagttc ttaggttgaa acggcataat gaagcgaata attagcaaga atcagatatg
      541 aatcttcacc aatttcgctt tgtccaggaa gccgtccggc gcaacctgaa cctcaccgaa
      601 accgcacgca tcctgcacac ctcgcagccc ggggtgtcca aggccatcat cgagctcgaa
      661 gaagaactgg gcgtggaaat ttttgcccgc cacggcaaac gcctcaagcg tgtgaccgaa
      721 ccgggcgaac atgtgctcaa aagcattgac atcatcatgc gcgaggttgg caacctgcgg
      781 cgtatcggcg agcagttttc agcccaggac agcggcaccc tgtccatcgc caccacccac
      841 acccaggcgc gctacgtgct gccgcagccg gtggccaaac tccgtgaagc gtttcccaag
      901 gtcaatgtca gcctgcacca gggctcgccc gaccaggtgg cgcgcatgct gatcgacgag
      961 gtggccgaga tcggcattgc caccgagtcg ctggcgcatt atgaagaact ggtcacgctg
     1021 ccctgctacg aatggcagca catgctggtg ttgccgctgg accacccgct ggccaaacag
     1081 gacaacatca cgctggaaga cctggccgag cagccgctga tcacctacca cccctcgttc
     1141 accggccgca ccaagatcga ccaggccttt gccgccaaac acctgcatcc gcgcattgcg
     1201 ctcgaagcga tcgactccga cgtgatcaag acctatgtgc gcctgggcct gggggtcggc
     1261 attgtggccg agatggcgct caaggacgac ggcaccaaca ccgacctggt cgccctgccg
     1321 gccggccacc tgttcggcgt gaacgtggcg cgtgtcgcct tcaagcgcag tgcctacctg
     1381 cgcaactttg tctacaagtt tgccgaactg ctcaatgaca agctcgaccg cgtccagatc
     1441 acccgggcca tgagcagccg ccacggcgac ttcgaactct aaattccatc actccaccct
     1501 caccatgacg accatgcctt ctgccagcga gcgcacgcca cagatacaga ccaaactgcc
     1561 cgccgtgggc accaccatct tcacggtgat gtcggcgctg gcccttgaaa aaaacgcggt
     1621 caacctcggc cagggcttcc cggacttcga ctgcgacccg aaactggtcg gcgccgtcaa
     1681 cgatgccatg acacgcggcc tgaaccagta cccgcccatg cccggcgtac cggtgctgcg
     1741 cgaggcgatt gccgccaagc tgcagcaact gcacggccgc agctacaacc ccaacaccga
     1801 aatcacggtg acggccggcg ccacgcaggc catcatcacc gcggtgctgg ccgtggtgca
     1861 ccccggtgat gaagtcatcg tgctcgagcc ctgttacgac agctacgtgc ccaacatcga
     1921 gctggccggc ggcgttcccg tgcgcgtgcc cctgacgccc ggcaccttcc gccccgactt
     1981 cgacaggatc tccgccgccc tcaccgccaa aacgcgcgcg atcatcatca actcgccgca
     2041 caaccccagc gccaccgtct ggacccgcga ggacatgttg cgcctgcagg agatcctggc
     2101 gcccaccgac gtgctgctga tcagcgacga ggtgtatgaa cacatggtgt tcgacgccgc
     2161 gcagggccgc gtgcacgaaa gcgcagcgcg cttccccggc ctggccgcgc gtgccttcat
     2221 cgtcagcagc ttcggcaaga cctaccatgt gaccggctgg aaaatcggtt atgtggcggc
     2281 gcccgcgtcg ctgacgaccg agttccgcaa ggtacaccag ttcaatgtgt tcaccgtcaa
     2341 cacgcccatg caatacggcc tggcgagcta catggctgat gcacgtccct acctcgacct
     2401 gccggcgttc taccagcgca agcgcgacct gttccgcgcc ggcctggcga aaacccgctt
     2461 caagctgctg cccagcgaag gcagctactt ccagtgcgtg gacatctcgc aagtcagcga
     2521 cctcggtgaa gccgacttct gcaaatggct gaccaccgag atcggcgtcg ccgcgattcc
     2581 cttgtcggcg ttttacggca acggcttcga ccagcgcgtc gtgcgcttct gttttgccaa
     2641 gaaggacgag acgctcctca gtgcgctgga gcggctggcc aggctgtaat ccgctatcac
     2701 attgatagct ggttgcgcac agcctataag ggctggaggc cgatttcatc agtgaagtcg
     2761 gcttgagaca cattcgcggc atcacgctgc gcctatggtc ctttctggac agtaggacgg
     2821 actccccact cccaccccta gcccctgccc tcgccccgct gggcgatgga gggggacttc
     2881 atttg
//
LOCUS       DAIDFG010000367         2885 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_367_length_2885_cov_13.654770, whole genome shotgun sequence.
ACCESSION   DAIDFG010000367 DAIDFG010000000
VERSION     DAIDFG010000367.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_367_length_2885_cov_13.654770"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          498 a          889 c          933 g          565 t
ORIGIN      
        1 ttgtaccagt cccagctgtt gagaacgatc tcgtcaaggg tctgttcgcc ggccagcacg
       61 ggggacagca ggatgcggtt gtagttgggg tgcggctcgg cgccgaacac cgtgatgtca
      121 tacagctcgg gcgcaacctt gagcagttcc tcgatggtac ggacgcccgc catgccgttg
      181 ccgaccatga ccagttttga ttttttcatc gtgccactcc agtgggggcc ggtgccgtcg
      241 gcgcaagcgc caaagccgtg ccggcttgcg aaaaatgggg ggtaaagacc atgatgttct
      301 ctcctgcaaa acagggaccc ggctgtggcc ggtatgaggt acaccaatac gcacgtcgtt
      361 atgcgcagaa cccgcagtgt gctggttgcg ggtgggcgcg tcgttgcgcc cgtgaaggct
      421 tacgcaaaag ttgtgccatg aggcggcgtg agtggcgagc ctcatggcgc gcccgtggcg
      481 tgcccgggag ggctggcatg ttctgcccat gcgcggcatg cctgggctgg cgcagtgctt
      541 gttgcaacat tgctccggaa ggctcgttat ctgcaccaaa ggcgggcatg catagggctt
      601 gtcatggtgc aggacgtgcc ccccgtcgcg ggtagcagac cggaaagagg ccttgacgcc
      661 taaagcgcga tgcccagcac ctcggtgccg tccacggcgc cgctgcggcc tggcagggaa
      721 aagcgcttgc gccgccccgt cttcacggcg ccggcgacct cgcgcagcaa ggtggcgctc
      781 gccgcaatct tccagccggc cgaggtggcc tgcttctgca gttgcagggt ggtgctgacg
      841 ctgtcgccga ccggcagaac ctgggcgttg ccccacaacg ggtcctccag tttggccagc
      901 gtcacggggc cgctgttcag gcccacactg atttcaaaac gcggcagcga gcgcccggaa
      961 aaatgactga gcaaaaagcc ctgaacccgc tgggcggcgt cgatcaggcc cagtgccgcc
     1021 cgggtcgcac gcaggccgtg gttgacggtg tcggtgtccg tgctgtccac gaacacggcc
     1081 agcaggcctt cgcccacaaa ctgcatgtgg aagacgccga aaagatagag ggtatcgccc
     1141 gcattcttgt aaaactcgcg cacgacctcg ctgagctccg tgctcgagag tttttcagcc
     1201 agcgccggga agttgaccac gtcgacaaag agaacgctgg cgctggaaaa cctgtggtcc
     1261 gcgaccaacg tttccgccga gggccatttg tcggcgagtt cagccagcat tcttttttca
     1321 tagaggctgc ccagcttttt ttcctgttcc ctcagggcct gcccgatggc tgcgccgacc
     1381 gcctgctcct ggacggactg gcgcatcagc tgcttgttga gctgggtggc ggttgctgcc
     1441 agcagttcct cgaaaaccag gggtttggtc aggtagtcgt ccgcgccgct ggtcatgccc
     1501 gtgcggacgt gggcgcgttc ctgcagcgag gtcagcagga tgacgggtgt ggcctggatg
     1561 gcggggtcgt tgcgcagcgc ctgcagcatg gcaaaaccgt ccatcacggg catgttgaca
     1621 tcgctgacca ccaggtcggg tgcgttgccc cgcaccagcg ccagcccggc ggcgccgttg
     1681 tccgcctcgg caacctcata accttcaaag cgcaaggcag cagcgatcag ggttcggatc
     1741 gcctcatcgt catcgatgac cacaatcagg gccatctctt tattccatga aaacaagcgt
     1801 catggctgca agcttaccag cgctgccggc atactggcga gatgccccac gttcgccagg
     1861 ccggcctgcc cccgttcgat ctgccgcggt gtcagtcaca cagggttgtg cgccctgcgc
     1921 ttgccggtca cgcggctttt tcgacgtggc cctggcgggt gtagaggaaa tcgatcaccg
     1981 ccttgcggta gtgcagatac tcggagcttt cggccagcgc cacccggtcg cgcggccgcg
     2041 gcagcgccac gctcagcact tcgccgatgg tggccgaggg gccgttggtc atcatcacga
     2101 tgcggtcgga cagcagcacc gcctcgtcca cgtcatgcgt caccatcacc accgtgctct
     2161 gggtgttggc aacgatctgc agcagctcat cctgcagctt ggcgcgggtc agtgcgtcga
     2221 gggcgccaaa cggttcgtcc agcagcaaca ccttgggctc catgcacagg gcgcgggcaa
     2281 tgccgacccg ctgcttcatg ccacccgaga tttcgccagg gcgcttttgc gctgcagcgg
     2341 tgaggcccac catggccagc gccgcgtcgg tgcgggcctt gagttgcgcc ttgttctctt
     2401 tggctgcaaa cacgcgctcc accgcaaggt agacattttc aaagcaggtg agccagggca
     2461 gcagcgagtg gttctggaac accacggcgc gctcggggcc cgggccggag atttcgcggt
     2521 tggcgcacag cagcgtacct tgcgttggcg tggtcaggcc ggcgatcagg ttgagcagcg
     2581 tggacttgcc acagcccgag tggccgatca gtgccacaaa ctcgcccttg gcgaccgtga
     2641 ggttgatgtt ctgcagtgcg cagaacgggc cttttttggt cttgaaggtc tgctcgacgc
     2701 cctggatctc gatgtatttg gtggtcaatg aactcatggt gcgttcctgg ttggcaggcc
     2761 ggaggcctaa cttttcactt cttcaaacgt gaaggccgtg gcgatcttga tcagcacaaa
     2821 ttccagcacc agcccgacga tgccgatcac gaaaatcgcg atgatgatgt tcttgacgtt
     2881 gaggt
//
LOCUS       DAIDFG010000368         2876 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_368_length_2876_cov_17.702233, whole genome shotgun sequence.
ACCESSION   DAIDFG010000368 DAIDFG010000000
VERSION     DAIDFG010000368.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_368_length_2876_cov_17.702233"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          511 a          889 c          934 g          542 t
ORIGIN      
        1 gcggcgtgcc ggcgcggttc aggtcaatgc cgctcatgcc gatgaagtct tcatgcagcg
       61 acttgcctgc gtcttcctcc aggtgggcgc gcaccagacg cacggttttt ttctcgtcgc
      121 cgaggaaaaa ttccaccgtg ccgccttgca ccaccgggat ctcgaactgg ctgatctggt
      181 aacccttggg caggtcgggg tagaaatagt tcttgcgcgc gaacacgctg ctttcggcaa
      241 tatgcgcgtt gacggccagg ccgaattcga tggcgcgttg caccgcgccc ttgttcatca
      301 ccggcagcgc gccgggcagt gcaaagtcca ccgccgaggc ctgcgtgttc ggttcggcgc
      361 caaacgctgt cgaggcccgg ctgaaaattt tcgattgggt ggtcagctgg gtgtgggtct
      421 cgaagccgat gatgacttca taaccctgca ccaggggccc ggtgggccgg ccggcctgca
      481 tctgggcgaa ggagttcacg gggggtgggg tctggctcat ggtcgcaatc actcaaatgc
      541 ctgtgggctg gcgcaggtgc cagtcggtcg cgagctggaa ctggtgcgcc gtgcccagca
      601 gctgcgcttc cttgaaatag ttgccgatca gctgcaggcc gacaggcatg ccgccttcgc
      661 cgaaaccggc cggcacactc atgccgggca ggcccgccag gctggacgac agggtgtaga
      721 tgtcggccag gtagttggcg accggatcgt cggatttttc gccgatcttc caggccaccg
      781 tgggcgacac cggcccggca atgatgtcgc actgcgcaaa ggccttctgg aaatcctcgg
      841 cgatcaggcg gcggattttc tgcgccttca ggtagtaggc gtcgtaataa ccgtgcgaca
      901 gcacataggt gccgatcatg atgcggcgtg tgacctcgtc gccaaaacct tcggaacgcg
      961 acttcttgta catgtcggtc aggtcggtgt agtgcgcggc acggtggcca taacgcacac
     1021 cgtcaaaacg gctcaggttg ctgctggcct cggccggggc gatcacgtaa tacaccggga
     1081 tcgacagctc ggtcagcggc aggctcacgt ccaccagcgt tgcgcccagc ttctcgaact
     1141 cggccagcgc gctgcgcacg gccttgagca cgtcggccga gcagccggcg ccgaaaaact
     1201 gggccggcag gccgatcctc aagcctttca ggggtgtagc gtccgtcgtt gctgcgctga
     1261 cggctatcaa atcggtagca tagtcgagcg ccggcaggtc cagcgaggtg gagtcgcggt
     1321 ccgggtcggg cccggccatg ctggacagca gcagggcgca gtcgagcgcg ctgcgtgcca
     1381 tcggtccggc ctggtccagg ctggaggcaa aggccaccat gccgtaacgc gaacagcggc
     1441 cataggtcgg cttgatgccg gtgataccgg tcagcgcggc cggctggcgg atggagccgc
     1501 cggtgtcggt gccggtggcc gccggcacca gccgcgccgc cacggccgcg gccgagccgc
     1561 ccgaggaacc gcccggcacg cggctggtgt cccaggggtt ctgcacgggt ttccaggcgc
     1621 tgttgtcgtt gcccgacccc atggcgaact catcgcagtt gagcttgccc aggctcacca
     1681 tgccggcgcc gctgctcacg ccgagccggg ccaccacggt ggcgtcgaac gggctgcggt
     1741 agttggcgag catcttcgag ccggccgtgg tcgggaagtc gcgcgtgaca aaaatatcct
     1801 tgtgcgccag cggcacaccc agcaggggcg tgcgttcgcc ggcggccagg cgcgcgtcgg
     1861 ccgcccgggc ctgggtcagc gacacctcgc cgtcggtggc caggaaagcg cccaggccgg
     1921 catggttgtc gatacgggcc aggaagtgtt gcgtcagctc gacgctggac acctcgcgcg
     1981 ctgcgagctt ggccgcgagt tgggccacgc tcaaatcatg caaatcggta tggctcattc
     2041 gatcaccttt ggcaccaaaa acaggccccg ctcgacagcg ggggcgcttt tctggctttc
     2101 ctcgcgctgg ttggtttcgg tcacgacgtc atcgcgcagc ctgagggcca cctggcccag
     2161 gaccgcggca ggatgggcca gcggttcaac accttccgtg ttgacggcgt ccatctgttc
     2221 gacgagggca aaaaaaccgt tgagctgcct gagcgtgtgc tcggtctcgt cggggcgcaa
     2281 ctccagcctc gccaggttgg cgacgcgtga tatgtcttgg gatgtcaatg ccatgggaaa
     2341 aacaggggct gaaaccaggt gtaaagatga aataatgggc agggtgccgc ccggtgattt
     2401 acaggggatg cgttattatc ccgcctttga tgtgaaatca gggtcggatg cacgctttag
     2461 gcgcggttta cgctctggaa tcaaggtgtt gcctcatttt cctgtccatc gacttttttt
     2521 gacccctcac aacaatcaca aaaggcgata gagcaacgaa gtttgttcca tgcccaaaac
     2581 cggactccct caccatgttt gaaactttcc gtcggtattt ttccaccgac ctggcgattg
     2641 acctcggtac cgccaacacc ctgatttttg tacgcgaccg cggcattgtg ctggatgaac
     2701 cttccgtcgt tgccattcgc cacgaaggtg gcccccaagg caaaaaaacc atccaggccg
     2761 ttggccatga agccaaggcc atgctcggca aggttcccgg caacatcgaa gccatccggc
     2821 ccatgaaaga cggcgtgatc gccgacttca ccgtcaccga gcagatgctc aagcag
//
LOCUS       DAIDFG010000369         2793 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_369_length_2845_cov_18.763441, whole genome shotgun sequence.
ACCESSION   DAIDFG010000369 DAIDFG010000000
VERSION     DAIDFG010000369.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_369_length_2845_cov_18.763441"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          522 a          924 c          880 g          467 t
ORIGIN      
        1 caagtcgggc aaggaagagc agtacctcaa agacggcccg gccctcgaca gcttcctgct
       61 gaagatagcg ctgaaagacg ccgctgtgtt gaccggtgcc gaccccgcca atggccaggg
      121 cacgctgctg agcggcgaca cgctggctga gctggcgcgc aagcaccagc tggccgagtc
      181 cgtcattgcc cgcctgcgcg gctacatgga cgccgaagcg ctcaaagcca ttgccgacgg
      241 cgtggccctg aacctcgaca cggtggaaga cgccgagcgg tccgctgccg cgctgcaggc
      301 ccagttgccc acggccgaag tggccggcga gtttgacagc cgcaacgaca aaccgattct
      361 gcgcatcagc cgccgtcacc acggcaacgt gaaaagcagc gtgctcacgc aggactttgt
      421 gcacggcgcc gactacctgg cactggtcga agctgcggtg accttcaagg gcctgatgag
      481 tgaaggcgcc aaggtgacgc gcggcgaagg cgagcgccag aaggaagaaa aagtcgagac
      541 cttccgcgac gccatggcct ggctgatcac gcaggccgaa gccgccaccg cacgccagcg
      601 ctacaagggc ctgggcgaga tgaatccggc ccagctgtgg gaaaccacca tggacccgac
      661 cgtgcgccgc ctgctgcgtg tgcagatcga cgacgcgatc gaagccgatc acgtgttcac
      721 catgctgatg ggcgacgacg tcgagccgcg ccgcgagttc atcgagacga atgcgctgcg
      781 cgccgcgaac attgacgtct gagttgctca gcgcataatt tgcagcatga acattgcacc
      841 ggcccaacac tcacagatcg ccgagatctg caggcgttat cacgtccgca agatgagcat
      901 gtttgggtct gcagcaatag gccaggagcg tcctgagagc gatgtcgatt tgctggtgga
      961 gtttattccc ggagaggctc cgagcggctt tgccctggtg gacatgcaag acgagctgtc
     1021 tgccgcgttc gatggacgga aaattgactt ggcttttcct tccattctcc gcaatgccta
     1081 ccggcgcatg gccattgagc cgcaattgcg acccgttttt gaatgacgat tgatcgcacc
     1141 ccggcacatc tggccgatat gctgcagttt gtgcaggagc tccgcggatt gctcgcagaa
     1201 cagcctcttg aaaagtttct ggcgaaccgt gttctttgtc tcgcgactga aaaactgttt
     1261 atcaatctcg gcgaagcggc cagccgcata gacgtggcgc gttgtgggca gatgcccgat
     1321 gtgccgtggc ggcaggtgat cggcttgcgc aacatactgg cgcatgggta tgagcaggtt
     1381 gaacatgaaa ccctgttcaa caccgtctcc cgggatctgc ctcaactcga agaggcgcta
     1441 agccaggagc tgctgaagta tcagctgcgt tgaggcgccg gccgcacatg acccgcatcg
     1501 ccgtcatcga ctttgaaacc accggcctgt cgcccgccat gggcgaccgc gccaccgagg
     1561 tggccatcgt gctgcttgaa aacggttgcg tggtggaccg cttccagagc ctgatgaatg
     1621 cgggcgtgca cattcccgcc ttcatcacca gcctcaccgg catcagcaac gccatggtgg
     1681 ctgcggcgcc cgaggctgcc ggcgtcatgc gcgaggccag ccgcttcgtc ggcaacgcac
     1741 ccatggtggc gcacaacgcg tcgtttgacc gcaagttctg gcaggccgag ctggcgcgtg
     1801 ccggcgcggc ggcgttgcag ccctttgcct gcaccatgct ggtcgcgcgc cggctctacc
     1861 cgcaggcccc cagccacaag ctcggcgtgc tggtggacta ccaccacctg ccgcgcgccg
     1921 gccgtgcgca ccgggcgctg gccgatgcgg aaatggcggc atcgctgctg gggcagatcc
     1981 agcacgacct gcgcacccgc caccgcgtgg gcaagcccga ccacgccttg ctcatgaccc
     2041 tgcagcgctg cgcgaaacct gccgttcctg cgctgatggc gaagtacgcc agtgtgtgag
     2101 cggcgctgaa tcgctattaa aagcgtagct ggttgcgccc gcccccaaag ggctggagtc
     2161 ataaaacgct cataatctgc cccatgccct cccataccca ccccgccacg ccgctgtcca
     2221 cccacgacac cacccgcatc gacgacaccc gcataggcgc ggtgcgcccg ctgatcacgc
     2281 ccgccttgct gcaggagtgg ctgccggtgc ccgaagccag cctgagcctg gtggagcgca
     2341 gccgcgcggc catctcgcgc gtgctgcatg gcgccgatga ccggctgatc gtcgtggtgg
     2401 ggccctgctc catccacgac catgaccagg ccatggacta tgcccgccag ctcaaggtgc
     2461 aggccgacgc actggccgac gacctgctgg tggtgatgcg tgtgtacttc gagaagccgc
     2521 gcaccaccgt gggctggaag ggctacatca acgacccgca catggacggc agcttcgcca
     2581 tcaacgaagg gctggaaatg gcgcggcgcc tgctgctcga cgtgctggct ctgggcctgc
     2641 cggtgggcac cgagtttctg gacctgctga gcccgcagtt catcagcgac ctggtgagct
     2701 ggggcgccat tggcgcgcgc accaccgaaa gccccagcca ccggcagctg gcctcaggcc
     2761 tgagctgccc ggttggtttc aagaacggca ccg
//
LOCUS       DAIDFG010000370         2790 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_370_length_2790_cov_16.725411, whole genome shotgun sequence.
ACCESSION   DAIDFG010000370 DAIDFG010000000
VERSION     DAIDFG010000370.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_370_length_2790_cov_16.725411"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          519 a          950 c          926 g          395 t
ORIGIN      
        1 ggccaccggc acgcaggacg cggtcgcaca gcgccgtcat ttccagatac ggcgagacgg
       61 gctcggccac gcggcgcagg tcgccggtca gctcgagctg ggccatgaaa tcgcgcaggt
      121 cgcggtaacg catggctcag gacccgtcgc tggcgggtgg tgtctgaccg tcccagcgca
      181 ccgccaactg gtggggtagg tccaacaagt ccagcacacg cgccacgctg ccgtccacca
      241 actcggccac cgactgcggg cgctggtaga aagccggcat gtgcgggcag atgatggcgc
      301 ccatttcggt cacgctgacc atgttgcgca ggtggcccag gtgcagcggc gactcgcgca
      361 ccatcagcac cagtcgccgg cgctccttga gcaccacgtc ggcggcgcgc gtgatgaggt
      421 tgtccgccag cccgtgcgcc acggcagcca gcgtgcgcat cgaacagggc gccaccacca
      481 tggcactgca taaaaaggag ccgctcgcaa tgcaggcgcc cacatcgcgc gcgtcgtgca
      541 cctgatcggc cagggcttcg acggcgacgc ggctcatgcc ctgctcctgg tcgaggttgc
      601 gccagcccgc gtcggacacc accaagtggg tttcgatccc ggccagggca tgaagcacct
      661 gcaacaggcg tgtgccgtac accgcgccgc tggccccgga gatcgcaaca atgatgcgcc
      721 ggggtgggcg cgcgctgtca ccggtgagca tgttcgtccg ctcctctggc ggcaaccccg
      781 ccaccggaag tggacgcacg cagcggcacc gccagcaggt cggccgccac acgctcgagc
      841 accagcgccg cctgcgcggg atcgaaatcc tcatgcgtcc gctttttgac gccgtattgc
      901 tggagtgggt aatgccggtc ggccgtgatg ccatggcgcg ccagcacgct tttgacgcac
      961 gccagcgggc agccgtcgag cgcgatgacg ggccgcccgg atcgcacgat tttcatcaga
     1021 tggggcacgt cgccacccac cccggcaatg caggacatct cggccacgcc gcgccggtcc
     1081 agctgcagcg ccacctggtt ggccagctgc gcggcgctgg agcatcccga acaggaatac
     1141 accagcggca aaccgtgcct atcctcagtc atggcggtta cctgcatgct gacgcgggcc
     1201 gacaccggcc gaccgctgcc gtggacccag gcgcgccagc aactggagcg gcgtccactg
     1261 ctgcaggtcg agcaccggca actccagggc atcaagcgcg ttcttgacga ccaggcccag
     1321 ttcggtgtcg ccctccatgg cgaggcgacg gctgaaaaaa agcgtgtccg gatcttcctg
     1381 gcgctgcgcc aggcgcatga aatcgtgggc gctggcactg atggccaggt cggtggcctg
     1441 ctggcggggg caggcgacga agcgctgccc ggtccaggaa aagtcaaagg tcaggcgcgc
     1501 gtcacgcaca tgaatgcgca gctttctgtg cagtaacagg cggcccacgt cggccggcag
     1561 ctggtgcgcc aggcccaggt tgagcgcgct caccagtagc accgagccgg gataagccgg
     1621 caggcgctcc agcagcgacc cgagggccgg cggcaatgcc aggggaggag atgaggaaag
     1681 gggtttcatg ggggtccgca aagtcaggcc acatacagca gcacggtgat gaagtggcag
     1741 atgctgccgc cgagcacaaa caggtgccac agaccgtggc catggcgcag cttgtggtcg
     1801 gtggcgtaaa aaccaatgcc cagggtgtag caggcaccgc ccgcggccag ccaggcaaag
     1861 cccgggggcg tcagggcggt ccacaagggc gacgccgcca ccagcgccaa ccagcccatc
     1921 agcacataaa tgaccagtga cagcacacgc gcgcccttgg ccagccagat ttcctgcacg
     1981 atgcccagca gcgccaggct ccacaccacg cccagcaagg accagcccca ggcgccgcgc
     2041 agagacacca gcgcgaatgg cgtgtagctg cctgcgatca gcaggtagat cgaacaatgg
     2101 tcgaacttgc gcagcacgtt tttggcgcgg ccgcgtatgc tgtgatacag cgtggaaaag
     2161 acgtacagcg ccaccagcat ggcgccgtag atgctgaagc tcacaatttt ccagggatca
     2221 ccaacgaagc tggcgggcac gatcagtgcg accgaacccg ccaccgccag cccggcgccg
     2281 acgaggtggc tgatgctgtt gaaacgttca ccgtggtaca tcgttgtcag ccctcagcca
     2341 gctaccgcgg aggccacctg ctccagcccg gggcggccgt gccagtagcc attgcagccc
     2401 ttctccggca tgaggggctg catgcgcgcc agcgcctcct gaggcgtcag tacctgcttg
     2461 cgtgctgcat cgaacagggc gatcacctcg gccatgtgct gcgcctgcgg actcaggcgc
     2521 accacatcca cgcccgcgaa acgcatgtcc tccagcgcat cgaccaggtt gtagacccgg
     2581 gccgactggg tctgcgtgcc gttgagcacc aggaactcct cgctctcgcg ggtcctgagc
     2641 atcaggccgt ccgggtgatc gaggcaactg aagcggcagt cgtccttggg caggttgcgc
     2701 tgccgcgcgg taaagcagcg cgccgagtag gccagcggca tgcggccgta ggcaaacacc
     2761 tcggtttcca gaccgggcgg gcggtcctgc
//
LOCUS       DAIDFG010000371         2747 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_371_length_2747_cov_18.939079, whole genome shotgun sequence.
ACCESSION   DAIDFG010000371 DAIDFG010000000
VERSION     DAIDFG010000371.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_371_length_2747_cov_18.939079"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          500 a          908 c          847 g          492 t
ORIGIN      
        1 gttgatcttg tcctcgacac tcgggcagta gcgcgggccc acgccgtcga tcttgccggt
       61 gaacatgggg ctgcggtcaa accccgagcg gatgatctcg tgggtgcgct cgttggtatg
      121 ggtgatccag caaggcatct gcctcgggtg ctggatgcgc ccgcccatga agctgaacac
      181 cggcaccggc ccgggcgtgc cacccggcat gccgtcgccc ggctgcaccg tgcacttgct
      241 gaagtcgatg ctgcgcccgt cgatgcgcgg cggtgtgccg gttttcaggc ggccttgcgg
      301 cagcttgagc tctttcagac gcgcgctcag cgacaccgcc ggcggatccc ccgccctgcc
      361 cgccgcatag ttgttgagcc cgacatggat cttgccgtcc agaaatgtcc ctgccgtcag
      421 caccacggtg cgcgaacgga agcggatacc gacctgcgtg actgccccga cgacccggtc
      481 gccctcgacc atcaggtcgt cgacggcctg ctggaacagc cagagattgg gctggttttc
      541 gatcatgcgg cggatcgccg ccttgtacag aatgcggtcg gcctgcgccc gggtggcacg
      601 cacagccgga cccttggagc tgttgaggat gcggaactgg atgccgcctt cgtcggtggc
      661 cagcgccatc gcgccaccca tggcatcgac ttccttgacc aggtggccct tgccgatgcc
      721 gccaatcgag gggttgcagc tcatctggcc cagggtctcg atgttgtgcg tcagcagcag
      781 ggtcttgcag cccatgcggg cagcggccag cgcggcctcg gtgccggcat ggccgccacc
      841 gaccacgatc acgtcaaatt cttgcggata tacataaggc gagtgcatca cgcctccttc
      901 aataaagcag tcgtcaggcg aggtgcctgc acgccctgga aaagaccggc cgggccggag
      961 aagtcagcaa acagcttgcc gctttcacag gacgaaaagc atgattttat cagggtgcct
     1021 cttttttgtg cggccggccc ccaacaaggc caatgccatt ccttctaccg gcaaggcaaa
     1081 ctgatcgcta tgattttaat agctattcgc gcttacctca taagggctaa acgcctaaat
     1141 ccttgatggc gcaggcggac agcagcgttc cggcccgcca ggcgcggcac gcaagcacaa
     1201 tagagggatg aaacttctga tcttcgccgg cagcacccgg ctcgcctcct tcaaccgcaa
     1261 actcgcccat gcagccgccg atctggcgcg cgccagcggc gccgacgtga cccacctcga
     1321 actggcggac tttgacatcc cgatgtacaa cgccgacctg gaagccaagg gaacaccagc
     1381 cgacgtcatg aagctcaagc agctgatgtt cgagcacccg gcctggatca tctgctcacc
     1441 cgaatacaac ggcagttaca ccgccctgct caagaacacc atcgactggg tttccagtcc
     1501 ggtcaaggcc gacccggcct ggcaggaagg tttcaagtcc ttcagcggca aggtcgtggg
     1561 catgctcagc gcctcgccgg gcgcgctggg cggactgcgt tcgcaaagcc acctggtgcc
     1621 gctgctgctc aacgcgcaat gctgggtcgc gccccaagcc tttgcgctgg gccatgcggg
     1681 tgatgcgttt gacgccgatg gcaagctggt caacgaaacc catcgcaaaa acgtacaggc
     1741 ggtgatcgac caggttctct ttgcgagcag ccgactccat gcttgaattg gcgttcagcc
     1801 cttattccac gggcgccaac agcttcctta ttaatagcaa aaggcatttg tgaactgccg
     1861 tcctggctgc ggtgcctgct gcatcgcccc ctccatcagc tccccgattc ccggcatgcc
     1921 gcagggaaag ccggcggggg tacgctgcgt gcagctggac gaggccaatg cctgccgcat
     1981 cttcggccag cccgaccggc cggccgtgtg tgggcagctg atgccatctg aggagatgtg
     2041 cggggacagt gccattgttg cgatggcgtg gctgacgtca ctggagcggg cgacggcacc
     2101 gagcctggca tcggtggcaa gccccggcga aatggcttgc tagcgaacta caaaccaacg
     2161 aagcacgaag cagtaagcaa acatggatcc cggatcaatt ccgggatgac ggatcagaac
     2221 ggatagtgcc gcggcgtcgt ctgtatcgtg atccagcgca gctcggtgaa ctcggccaca
     2281 cccgccttgc cgccgaagcg cccgatgccg ctgcccttga cgccgccaaa cggcatctgc
     2341 gcctcgtcat gcaccgtcgg gccgttgacg tggcagatgc cggagtcgat gcgcctggcc
     2401 acgttgaagg cgcgcgcgat gtcgccgccg aacaccgcgg ctgacaggcc gtaggcgttg
     2461 tcattggcac aggccacggc ctcctcgacg ccgctgacac gcaccacaca cttgaccggg
     2521 ccaaaggtct cttcgtggta gatgcgcatc gcaggtgtca catggtccag cacggtcgcg
     2581 ggcatcagcg tgttttcggc cttgccgccg caggccagct tcgcgccctt ggccagtgcg
     2641 tcgtcgatca gcgcattgca gtgctcgacc gtgcccatgc cgatcaccga gcccagcacc
     2701 accggctcgg gcttgcgcgg gtcaccgagc ggcaaggcgc tggcctt
//
LOCUS       DAIDFG010000372         2720 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_372_length_2720_cov_20.269418, whole genome shotgun sequence.
ACCESSION   DAIDFG010000372 DAIDFG010000000
VERSION     DAIDFG010000372.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_372_length_2720_cov_20.269418"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          443 a          874 c          923 g          480 t
ORIGIN      
        1 agggagtatg gggtgtagag cctcatcgag ggttcgaatc cctccgtttc cgccagacaa
       61 aaagccaccg caaggtggct tttttttgtg ctgctccaga tgccgccgcg cgtcgtgtcg
      121 caaccagtcg taaaaaagcg ggcgccgccg gggcgcagac gttatggtgg cgctttacct
      181 cccggagtcc ccatggtgtt aaagaccccc ctgatcagcg cgacgctggc atgtctggct
      241 gttctggccg gttgtgccgc gcctggcgca cctaccacgg gcaccagccc catgccgccc
      301 ccgcccccgg ggactgcctg caatgcggac ggagcccggt tcgtgatcgg caaggcaccg
      361 ggggcgagtg tggtcgagga agcgcgccag cgctcgggct cctacatggc gcgggtgctg
      421 cgtcccaacc aggccgccac catggaattc aacgcccagc gactcaacct cgagctggat
      481 gctgccggcg tgattcgacg ggtgcgctgc ggctgaaggc cgccgggcgc agtctgcgcc
      541 tgttttgttg aatgaaacac tcgtgctata tttcatgaaa tattgataca aatgtttcat
      601 gccgacctcc tcactgtttg ccaaccttcc gcttgatggt ctgagtccgg agttgctccg
      661 tgcggcgcgc tggatcgagg cgcatccgcg cgaggtggcg ctgcattcca tgcgtgaatg
      721 cgcgcgccgg gccgctctgg caccggccac cttgagccgc ctgtcgcagg ccctggggtt
      781 ccagggcttc gaggcgatca agcatctctg ccaggagtct ttttcagcgc aggccggtta
      841 tgccgggcgg gccgaggtgc tgcaggccag cgcgcggcat gacaaggact ggctggcgct
      901 gctcaatgaa acgcaacatg cgaacaccgc gtccgtcagc gggctgaatc aggccgcgca
      961 gctggaggcc gcggccgatg ccatgcttaa ggcgcggcgc gtctattttc tgggcctgcg
     1021 tgccagccac ggcctggcgc tgcacctgca ctacacctat ggcctgctgg cgtccaacgg
     1081 cgtgctggtg cagggcctgg gcggcacgct gaccgaccag atcggcgaga tgggcacggg
     1141 ggacctgctg gtggcgactt ctgccgccgc gtacacgcgc cagacggtgg acgccgtggt
     1201 gacggcgcgg gcgcagggcg ccgatgtgct gaccctgacg gacagtgccc tctcgccgat
     1261 tgcccgttcg gccacccaca cgctgctgtt ccgtaccgac agcccctcgt atttccattc
     1321 catggtcggc gccctggctt tgaccgaagc gctggccgcc gccgtggcgg tacgcggcgg
     1381 ccgcaaggtg ctggtctacc tgcaaacccg gcaggaccgc ctggacagcg agggcgccta
     1441 ctgggacgaa cgcgccgaca aggtcgccac tggccggcca ctgacttctt caccaaggaa
     1501 ataagcccat gcctgcagcc tccacccatg tctttcaccg ccagttgcag cacacgccgc
     1561 cggtggccgt cagcggccgg ggggtgtacc tgaccgatgc cgatggcaag agttatatcg
     1621 acgcctcggg cggcgctgcg gtgtcctgcc tgggccacgg ccatcccgac gtgctggccg
     1681 ccatgcatgc gcagatcgac aagctggctt atgcccacac cagttttttc accaccgagg
     1741 ttgccgagac actggctgcc caactgatca ggacggcgcc gcaaggcatg agccatgtgt
     1801 atttcgtcag cggcggctcg gaggcgatgg aggccacact gaagatggcg cgccagtatt
     1861 ttgtcgagat cggccagccg cagcgcacgc atttcatcgc gcgcaggcag agttaccacg
     1921 gcaacaccct gggcgcgctg gccataggcg gcaaccagtg gcggcgcgaa ccgttcgcgc
     1981 ccctgctgat gccggccacc catgtgtcgc cctgttatcc ctaccgcgag cagcaggccg
     2041 gcgaaacgcc ggagcactat ggccagcgcc tggcgcggga actcgaggac gccatccttg
     2101 aacaaggcgc agaccgcgtg atcgccttca ttgccgaaac cgtgggtggc gcgaccgccg
     2161 gtgtgctggc gccggtaccg ggttatttca aggcggtgcg cgaagtctgc gaccggtatg
     2221 gtgtgctgct gatcctggac gaagtgatgt gcggcatggg ccgtaccggc accctgcatg
     2281 cgtgcgagca ggagggtgtg gtgcccgacc tgttggccgt ggccaagggc ctgggcggcg
     2341 gctaccagcc gattggcgcg gtgctcgcgc agaaaaaaat tgtcgatgca atgcggggcg
     2401 gcaccggctt tttccagcac ggccacacct acctgggcca tgcggtggcc tgtgcggcgg
     2461 cgctggcggt gcagcaggtg ttcgagcggg accagctcat cgttcaggtg cgcgcgcggg
     2521 gcgcgttttt gcagacgctg atgcagggcc gctggggcga acatccccat gtcggggaca
     2581 tccgcggccg tggtttgttc tggggcatcg agctggtgca ggaccgcgcg aagaagacac
     2641 ccttcgatcc ggcacgcaaa gtgcatgcac gcgtcaagaa ggaggcgatg gcgcgcggct
     2701 tgatggttta cccgatgggc
//
LOCUS       DAIDFG010000373         2720 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_373_length_2720_cov_16.294184, whole genome shotgun sequence.
ACCESSION   DAIDFG010000373 DAIDFG010000000
VERSION     DAIDFG010000373.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_373_length_2720_cov_16.294184"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          405 a          939 c          902 g          474 t
ORIGIN      
        1 atcaccggcg gcggcctgct ggaaaacatt ccgcgcgtgt tgcccgaagg cacggccgcc
       61 catctgaaaa agggcagctg gccgcagacc gagctgtttg cctggctgca gaaaaccgcc
      121 gccatctccg acttcgagat gaaccgcacc ttcaacaacg gcatcggcat ggtggtggtg
      181 gtggatgccg ccagcgccct ggccaccgca aaaaccctgc gcgacgccgg cgagcaggtg
      241 tatgaaatcg gtgtgattgc acctcagggc gacggcgccg cggtcgcggt cagctagaac
      301 caggccccag gacgcaaggc acggggcagg cgctacagac cgcacggaca accccgccat
      361 gcccggcaca ccccccatcc cgccacggcg gcttccggcc gccatctggg cactgggttt
      421 tgtcagcctg ctgatggaca tctcctccga gatgatccac agcctgctgc ctgtcttcat
      481 ggtgacggtg ctgggcacca gcatgtgggc cgtcggcctg attgaaggtg tagccgaggc
      541 cacggccctg atcgtcaagg tcttctcggg cgtgctcagc gactactggg gccggcgcaa
      601 gccgctggcg gtgctgggtt acggcctggg cgccgcctcc aaacccctgt ttgcgctggc
      661 caccaccacc ggcatggtgc tggccgcccg cctgatcgac cgtaccggaa aaggcatacg
      721 cggcgcgccg cgtgacgcgc tggtagccga tctggccccg cccggcatgc gcggtgcggc
      781 cttcgggctg cgccagtcgc tcgacaccgt gggcgctttt gtcgggccgc tgctcgccat
      841 gggcctgatg ctgctctggg ccaacgactt ccgggcgatt ttctgggtcg ccatggttcc
      901 gggtttcatg gcggtggcct tgctgctgtt cggcgtacag gaggctgcgc cggcacagga
      961 caaaaccgcg gcgccacgcc gcaacccgat cagccgggac gagttgcggc ggctgggcgc
     1021 cccgtactgg cgcgtggtcg tgattggcgc cgtcttcacc ctcgcgcgtt tcagcgaagc
     1081 ctttctggtg ctgcgcgcgc tgcagggcgg cctgcccatc gcctggacgc cgctggtgct
     1141 gatcggcatg aacgtcgttt acgccctggg cgcctacccc ttcggcaagc tgtcggacca
     1201 gatgagccac accaggctgc tggcagcggg gctggtggtg ctgatcgccg ccgatgtggt
     1261 gctggcctcg ggcaaccact ggacctttgt ctgggccggc attgcgctgt ggggcctgca
     1321 catggcgatg acccagggcc tgctggccac catggtggcc gacgccgcac cggccgacct
     1381 gcgcggtacc gccttcgggt ttttcaacct gatgagcggc ctggccatgc tgctggccag
     1441 cacggtggcc ggggtcttgt gggaccagct gggcgccccg gtgacctttg tggcgggtgc
     1501 ggtcttcagc ctggcggcgt tggcgctgct ggggtgctgg cgcggggcgc gggtccgcga
     1561 tgcggccgcg gcctccccct gaataagact tttttgctac aaaaatacta gctgcttgcg
     1621 cccgcattca ttgggcagac tggacttttg aggcaatttg tatcaaactg ttccttctac
     1681 ccgattctgg agcgttcatg ccggtcatcg cagtcgtcaa ccgcaagggg ggcagtggca
     1741 aaagcacact ggccgcccac atcgcggcat ggtgcgcgcg caatggcagc tccgtcatgc
     1801 ttggcgacgt ggaccgacag cagtcggcgc gtgcctggtt gaaacggcgc gacggctcgc
     1861 tgccggccat cgcgccctgg gcggtggacc agaaaaacat gttgcgtgtg ccgaccggca
     1921 tcacccacgt cgtgctcgac acccccggcg gcatgcacgg ctttgaactc gctcgcgtcg
     1981 tgatgtttgc cgacgccatc gtgatgccgg tgtgcaactc gatgttcgac cgcgaatcgg
     2041 ccgaagcctg ccacgccgaa ctgatgacca tgccgcgcgt ggccagcggc cgctgccgcc
     2101 tggcggtggt cggcatgcgt atcgacgggc gcaccaatgc cgcgcaaacc ctcgaagaat
     2161 ggtcggaatc gctgggcgtg ccttttctgg gcgccctgcg cgaaacgcag ctttacgtgc
     2221 gcagcctgga gcgcggcctg acgatttttg acctgcagcc ccagttggcc gctgcagatc
     2281 tggcccagtg ggcgcccatc cttcagtggc tcaagcccca gctctacccg ccccaagccg
     2341 ccaacgacgg ggcaggcgtg gtgcgccccc cggcacgcac cctggcgccc aaaggtgcgg
     2401 catcaccctt gaaagcgccc ggcgaagtgg tgtccaaacg gctgggttcg ctgccgggca
     2461 gcctgatgcc ggcccaggag tccatggtcc agggcggccg catgtccacc ctgagcgccg
     2521 gtcgcaccac ccccgccccg gcgagccccc cggtcgcccc caccctggtc cgccccgcac
     2581 gcgcagccga cagcccgcag attccacagt ttttgaaacg cagcggttag cagagccccc
     2641 acggttgctc actgcgtgta gcgcccgagg ccttgcaggc cgcggctcgc gagacgcttc
     2701 gcctctgtcg cctgcccaaa
//
LOCUS       DAIDFG010000374         2714 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_374_length_2714_cov_17.217751, whole genome shotgun sequence.
ACCESSION   DAIDFG010000374 DAIDFG010000000
VERSION     DAIDFG010000374.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_374_length_2714_cov_17.217751"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          630 a          794 c          772 g          518 t
ORIGIN      
        1 ctacatcccc ctgccagagc gtgccgttga cggcgccttc ctgatgcccg tcgaagacgt
       61 gttctccatc tccggccgcg gcaccgtcgt gaccggccgt gtcgagcgcg gtgtggtcaa
      121 ggtcggcgaa gaaatcgaga tcgtcggtat tgccgacacc cagaaaacca tctgcaccgg
      181 cgtggaaatg ttccgcaagc tgctcgacca gggccaggcc ggcgacaacg tcggtatcct
      241 gctgcgcggc accaagcgtg aagacgtcca gcgcggccag gtgctgtgca agcccggctc
      301 catcaagccc cacacccact tcaccggcga gatctatgtc ttgtccaaag atgagggcgg
      361 ccgccacaca cctttcttta acaactaccg tccccagttc tacttccgca cgacggacgt
      421 gaccggtgcg atcgagttgc cagaaggcaa ggaaatggtc atgccaggcg acaacgtgtc
      481 gatcaccgtc aagctgatca acccgatcgc catggaagaa ggtctgcgtt tcgccatccg
      541 cgagggcggc aaaaccgtcg gcgccggtgt cgtggccaag atcattgcgt aattcgctcc
      601 acatttaagg aattaccatg gctaccaagc aaaaaatccg catccgcctg aaggcgtttg
      661 actacaaact gatcgaccag tcggccgccg agattgtgga tacagccaag cgcaccggcg
      721 cgatcgtcaa gggccccgtg cccctgccga cccgcatgaa gcgttttgac atcctgcgtt
      781 cgccccacgt caacaagacg agtcgcgacc agctggaaat ccgcacgcac cagcgcctga
      841 tggacatcgt tgacccgacg gacaaaaccg ttgacgcgtt gatgaagctc gatctgcccg
      901 ccggtgtgga cgtcgaaatc aagctgcagt aagcaagaag cggggcggcg gcgaaagccg
      961 gcgccccaaa aaacgaggtt tcagcgcaga tttgccatca ctggcagtct gcgctatact
     1021 cttgggctgt cctgaacatg ctgtgcatgg gtagggcagt tttattaaca gtctttcaca
     1081 tcaaaacgtc tgcggccaat tgaagtcgca gcggtggaag ttacggagaa aaaagcatga
     1141 gtctgagcaa ctccctcggg ttgctgggcc gcaaggtggg catgatgcgt ctgttcactg
     1201 atgatgggga taccgttcct gtcacagtgg tagatgtgtc taacaaccgt gtgacccagg
     1261 tgaaaaccca agcaaacgac ggctacgatg ccctgcaagt ggcatttggt gcgcgcaaag
     1321 cgtcgcgcat caccaagccg gccgctggcc accttgcgaa agcaggcgtt gaagccggtg
     1381 aaatcctgaa agaattccgc gtgacggctg acgtcgcagg caaatatgcc gctggtactg
     1441 ttgtgcccgc cgctgacgtg tttgcggtcg gccaaaaagt ggatgtccag ggtacttcca
     1501 ttggtaaagg ctttgccggc accatcaagc gtcacaagat gagctcgcag cgcgcgtcgc
     1561 acggtaacag ccgttcgcac aacgtgcctg gctccatcgg tatggcgcag gatcccggtc
     1621 gcgtgtttcc cggtaaacgc atgacgggcc acctcggtga cgtcaccaag accacgcaaa
     1681 acctcgacat cgtgcgtatt gacgaagccc gtcaactgct gatgatccgt ggcgctgtgc
     1741 ctggttccaa aggcggtttt gtcacggttc gtgccgcgat caaagccaaa ccagtagcag
     1801 ctgccaaagg agcgaactga tgcaggtcga actcctgaat gaccagggtc tggcttcgtc
     1861 caaagtggac gtgcccgata ccgtttttgg tcgcgaatac aacgaaagcc tgattcacca
     1921 ggtggtcgtg gccttccagg ccaacgcccg ccagggaacg cgtgcccaga aagaccgtga
     1981 gcaggtcaag cactcgacga aaaagccgtt caagcaaaag ggtactggcc gcgctcgcgc
     2041 cggtatgact tcttccccgc tgtggcgcgg aggcggtcgc attttcccga acatgcctga
     2101 cgaaaacttc acgcagaaaa tcaacaagaa gatgtatcgc gccggcatgg cttcgatctt
     2161 ctcccagttg gcacgtgaag gccgcctggc tgtggtggac tcattgaccg tggattcgcc
     2221 caagacgaaa gtcctggccg acaaatttaa ggccatgaac ctcaaatcgg tgctcgtcat
     2281 cgctgacgag gttgatgaaa acctgtacct ggcttcgcgc aatctggtga acattcttgt
     2341 ggttgaaccc cgctacgcgg atccgctgtc gctggttcac tacaaaaagg ttctggtcac
     2401 caaggccgcc atggacaaac tcaaggagat gtttgcatga gcgccgtgaa caccaaacca
     2461 aaatttgacg aaggccgcct gatgcaggtg ctggtcgccc ccatcgtgtc tgaaaaggca
     2521 accatggtcg cggacaaatc caacgccgtg ctgttcaagg tcctgcagga cgccaccaaa
     2581 cccgaaatca aggccgctgt ggaattgatg ttcaaggtcg aggtcaaggg cgtctctgtg
     2641 gtaaacacca aaggcaagac caagcgtttt ggcaaatcca ctggccgtcg ggacaacatc
     2701 cgtaaagcct atgt
//
LOCUS       DAIDFG010000375         2710 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_375_length_2710_cov_19.855367, whole genome shotgun sequence.
ACCESSION   DAIDFG010000375 DAIDFG010000000
VERSION     DAIDFG010000375.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_375_length_2710_cov_19.855367"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          503 a          977 c          869 g          361 t
ORIGIN      
        1 tgtcacgggc caaggccgtt atgcaccgca agaccgaccg cgcgctggcc atggacctgg
       61 agtcctgcgt caactgcggt tactgctccg aggcctgcca cttctaccag agcacccagg
      121 acccgaagta cacgccgacc cgcaagctcg acctgctgcg gcgcgtgcac atgcgcgaga
      181 cctcggcgtt tgcgcccctg caccgcctgt tcaagcccga catcacggtc gccgacctgc
      241 aggagtggca ggagctggtg tacgactcgt gcaccgaatg cggccgctgc agcatggtct
      301 gccccatggg catcaacatc gcgcgcggcg tcaacgtcat gcgcgaagcg ctgtccgaag
      361 ccggcctggt gccgctggaa ctgatggcgg tggcgcagga gcaggccggg cgcggcacgg
      421 tgttcggcgt cggccccgcc gagttgctca agacagtaga ggccctgcgt gcgcaaggca
      481 tcgccattcc cctggacgaa cccgccgccg atgtgatgct ggtcaccacg gtcatcgacg
      541 tgctgctgta ccaggacgcg ctggcggcca cggcgcgcat tctcaaccac ctgggcctgc
      601 gctggacgct gcgcagcgcc ggattcgaag cggccaactt cggcctgctg tcgggcaacg
      661 aagccctgca gaaggccgcc agccagcgcc tgatcgacga ggcactggcc ataggcgcgc
      721 acaccatcat cctgcccgag tgcggccacg cctacccggc cctgcgctgg gaagcccggc
      781 tgcccggcgg cgagccgctg cccttcgagg tgctggccgc ctcggaattc gtcggccgtg
      841 aaatcgcgag cggacgactg gaggtgcgct caggcgacac ccggcaggtc gtcacctacc
      901 acgacgcctg caaactggca cggcacggcg gcgtgatcga cgagccgcgc gcggcgatag
      961 cggccctggg ggtcgaattc cgggagacct cccccacggc agaactcaac tggtgctgcg
     1021 gcggcggtgc cggcaccttc ctgatcaacc gtgccgagcc gctgcggcac aaggcctggg
     1081 agatcaagcg ccagcagatc gacgccaccg gcgccgacgc agtggtggtg tcctgcgcga
     1141 gctgccgcct caacttcatg gccggtgcgg aaaccgacca gtggtccaca cgcatcgtca
     1201 gcctggtcga actggtggcc gaccacctgc ccggggctca cgcgcgctga gcggccagcg
     1261 ggccattggg gaacaaagca ggctttcgcc caacaccggc gggcgttggc tgctatcaaa
     1321 acaggatcgc accagcgctg aagcccccct gcagcgcacc gggcaacggt aaaccgctac
     1381 catgggcgaa tgaactctgc ccgcaccctg gtgaaaacac gtgaaacatg gcgtaaggcc
     1441 gcaaggggtc tggcgctagt cctgctggcc gccaccgggc aggcgcatgc agacagcgcc
     1501 agtcaaccgg gcctgctgcg cgacatcaac gcggtgcgac agcaaggctg cgaagggcag
     1561 cccggcaagg cagccccctt gcgcgagaac gcagccctgt cggccgccgc cacccgcctg
     1621 tccagcggtg acaggctgga cgaagcactg aaaaccgccg gttaccgcgc ggtgagcggg
     1681 gcgcagatca tcctgcgcgg cgcgggcgcc accctgctga cgcgggccag cctgggcacg
     1741 tcatgcagcg tgctcaccca ggccgatttg accgaggccg gctttcacca gtatgccgcc
     1801 cagacctgga tcgttgtggc cgcgcccttc tcaccccccg acgctgcgca ggccgctgac
     1861 gtggagtcgc gtgtactggc cctggtcaac gaagcccgcg ccaagccacg ccgctgcggc
     1921 accgagtctt tcgccgcggc gcgcccggtg catctcagcc ccagactgca ggccgtcgcc
     1981 gccgcccacg ccgccgacat ggcccaccac aactacttcg accacgccgg ccgcgatggc
     2041 aaccatgtgg aagaacgcgc cagcagcggc ggctaccgct ggcgccacat cggcgaaaac
     2101 atcgccgccg gccacaccca ggccgaagcc gccatgcagg actggctggg cagccccgag
     2161 cactgcgcca acgtgatgtc gccggcctac accgagatgg gcagcgcctt tgcggtgaac
     2221 aacagcagca gcgcgggcat ctactgggtg aaggtgttcg ggaccacgaa gtaaggcgcg
     2281 gcttgccacg ggcggcggcc cttcggtgat atgcaaaaat cgtcctgagc ccttatccgg
     2341 caagcgccgg cagcgatcaa aagaagagct gatactactt gtgtcggcgg ctttccggtg
     2401 gaaagcgaac ccacgccgcc accaccagca gaccgaacag cgagtagccg acggcaaaca
     2461 cccaccacgg tgcttcgtaa tacaggacgc gctgcaacca gtgttcgata aaaccgccgc
     2521 catagccggt cacaccggcc ataccggtcc gctcacgcag ccacatctcc agcgtggtca
     2581 gcgggcacaa catgcccagc caggtttcgg ccaccacgac cacgatcgcc atcaggtgcg
     2641 ccagccggaa ccagagggta ttgacccagc gccagccccg caggttgccg gccagcacca
     2701 gcaccaggcc
//
LOCUS       DAIDFG010000376         2705 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_376_length_2705_cov_15.170566, whole genome shotgun sequence.
ACCESSION   DAIDFG010000376 DAIDFG010000000
VERSION     DAIDFG010000376.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_376_length_2705_cov_15.170566"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          539 a          763 c          799 g          604 t
ORIGIN      
        1 cttgtgggtc tcaaagcctc gttcgccgcc gacccaggtg ttgccgctgt tggcaaaagc
       61 tgcgggaatg gctgcaccgg caagggcaag ggcaaatacg gttttacgaa acaacatgat
      121 caaactcctt taatatcgat tgcaaggtgt ggttccaaca cttggagcca ctgcgatgca
      181 aactctgcat cacagttgtt aatgtagaaa cccagctgcg acaccaccct gacccggaga
      241 ttacgaatcc gttatgtacg atcgaggctt gttttgaaaa ttctggtgat tgaagacgaa
      301 cccaaggccg ccgcctacct acgccaaggg ctgaccgaaa gcggttatgc cgtcgaggtg
      361 gcgtacaacg ggacggacgg gttgcacgcg gcggccaatg gtgaccatgg tttggtgatc
      421 ctggacgtca tgctgccggg catcgacgga tttgccgttc tgtcggcgct acgtacctcc
      481 aagcaagtgc cagttttgat gctcaccgcc cgcggcaaga ccgacgacaa ggtgcgtggc
      541 ttcgatctgg gagccgatga ctacctggtc aaaccgtttc agtttccgga gttactggcc
      601 cgagttcggg ccttgctcaa gcgcggccag cccgtgaccg gggagcccgt gctgcgtgcg
      661 gcggacctgg agatcgattc cgtacgtcac cgcgccactc gcgctggcca gcgcatcgac
      721 ctgtcggcca aggagttcgc cttgctgact cttctggccc agaaagccgg cgaagtcctc
      781 tccagaactc aaatcgcctc gctggtctgg gatattcact ttgattcgga cacgaatgtg
      841 gtcgaagtgg cgatacgacg gttgcgcgcc aagatcgacg accctttcga agagaaattg
      901 attcataccg tgcgcggcgt gggctatgtc ctggagcgcc gggccgtgcc gtgaagcaac
      961 gcttgctccc cagcttctgg ctcagaaatt ccctggctgc gcgcattgcg ctcgccagct
     1021 ctttttttgg tcttctgatc actggcggag ccatcgtggt cgggttctat gcactgacac
     1081 atcagcttga cgcgcggtca gtaacagagc tgctgggcaa gcgtgacttg gtgctgcatg
     1141 ttttatccga aatttcgtca ccggaagcgg tcaaccagaa caaacaccgc tttggcgatc
     1201 tgctgattgg gcacgatgac ctccacttgg cgctggtaga tcccgctagc ggtcaaagcg
     1261 tggcgagctt ttctgcgatt gcgcagcaat ccgtgtctgc gctggacgcc ggcccgaaca
     1321 gttccacgtc cgttcacgcg tggacggcgc cgactggtga acggctaaat gccattcacg
     1381 gcatcggtat ggccgccaat ggaaagtctg tgcgttacta cctgtcgctg gatcgccggc
     1441 acgacagccg attactggct gacttcatca aggcaactct ggtgggcttg ccgatactgc
     1501 tactcatcgt ggcgctgggc gcttggctca tcgcgagcac cagtctggca cctttgcggc
     1561 gcttccatcg gctggcggca tccatcggcg cacagtcgct cagtcagcga atatcttcct
     1621 caggtctgcc cacggaactc tacgaacttg cagaagagtt caacggcatg atggatcgca
     1681 tcgatgcggg ttaccggcgg ctgcaggatt tctccggtga gctcgctcat gaaatgcgta
     1741 cgccggttgc aacgctcatg gggcgaacac aggtggcact gtcccacaag cgcacatccg
     1801 ctgaattcaa agaagtcctg gaaggcaacg ttgaagaact tgagcgcttg tcccgcctga
     1861 tcgccgacat gctctttatt gcccgcgcag accacagcga aacattgctt cagggagagc
     1921 ttctggagct tgccaaggag gctcaacgag tggccgatta cctcttgctg atcgctgagg
     1981 agcgagggat tgcggtggag gtgacgggca ccgcgattgt tatggccgat cgcctgttgg
     2041 ttgagcgcgc gatcaccaac ctgttgtcca atgcaatacg ccatgccttt gcgaattcga
     2101 aggtaagcat agtgatcgcc acagacggcg actgcaccac cttggcggtg accaaccagg
     2161 gcgaggggat cgctcgagcc catctggatc gcatctttga tcgtttctac cgcgtcgatg
     2221 ccgcgcgcgc ccgcctcgat ggcggaacag gactgggtct ggcaattgtg cgatccatca
     2281 tgttggctca tggcgggcag gtgatcgcag acagccggcc gggcggcgag acgactttca
     2341 cgctggtatt tccagtagcc ggttttcctg cgccgaaact ggcctgaagt cctgcatgac
     2401 gagggtgagt gcgaggtgca tctcatcatt cccgatgagc accgcgcctg cagcgcagtc
     2461 gtattcgctg agaccctgtc gcaggtattc ggtcggcctg agttcgtctt tgaccgcaag
     2521 aagacacacg ggatttttta cttgttttac agctgcttgt gttttcggag agattacaaa
     2581 tttgtaatct cccggtaggt ctggcgcagg attcggatat ttaaactcgg ggtctctcaa
     2641 cctgtgatcg acacggatga cgcactttcg tcccgatggg cgaaaaattc atatctagga
     2701 agtgc
//
LOCUS       DAIDFG010000377         2698 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_377_length_2698_cov_16.593644, whole genome shotgun sequence.
ACCESSION   DAIDFG010000377 DAIDFG010000000
VERSION     DAIDFG010000377.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_377_length_2698_cov_16.593644"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          512 a          909 c          849 g          428 t
ORIGIN      
        1 ctctctgccc cccgaggggg ccgctgcgcc tgcggcccga caaagccggc tccgcggccc
       61 cggccggctt gaagacaacg ccaccgtact tttcgagctg tggcactgag actctttcag
      121 cgcgaacggc gcagcctgtt cggggagatc ctcgactgga tgctcacgcc gctgctgttg
      181 ctgtggccga tcagcctggc gctgacctgg ctggtggcgc agaacattgc cggcaaaccc
      241 tttgaccgcg cgctcgagta caacgtgcag gcgctgagca aactgatcgt cgtcaaaaac
      301 aagcaggtgc agttcaacct gacggcgccg gcacgcgaga tcctgcgtgc cgacgacacc
      361 gacctggtgt tctaccaggt gctgggcgcg cgcggcgagt tcatcagcgg cgaacacgac
      421 ctgcccctgc cgacagacga ggagatgacc tttgacggcg aagtacggct gcgcgaggac
      481 atcatgcagg ggcaggacgt gcgtgtggcc tacacctgga tcaaggtcga tgtggcgggc
      541 ggccacccga tcctgatcca ggtggccgag acgctggaaa agcgcaagac gctggccacc
      601 gagatcgtca agggcgtgat ggtgccgcag tttgtcacgc tgccgctggc cgtgctgctg
      661 gtctggctgg cgctggtgcg cggcatcaag ccgctggacc agctggaaaa acgcatacgc
      721 gcacgcaagt ccgacgacat gagcccgctc gacgaaagca tcgtgcccga agaagtcgcg
      781 ccactggtgt cgtccatcaa cgacctgctg ggccggctca aggtgctgct gaccacgcag
      841 aagcgctttc tggccgacgc cgcccatcag ctcaagaccc cgctggccgg gctgcgcatg
      901 caggccgacc tggcacagcg cgaaaccgac gccgacgaac tgaaaaagtc cctcaagcag
      961 atcggccgcg ccagcatacg cgccacccac accgtgaatc agttgctggc cctggcccgc
     1021 gctgaaacca ccggccgcag cctggccaag cagcggctgg acctggtgca catcctggcc
     1081 gaggccatcc aggactcggt gccgcgggcg ctggaaaaac gcatcgacct cggctacgaa
     1141 ggcccgccgg ccggcgaagc cgccaacatg ctggaagcca accccacact gctcaaggag
     1201 ctgacgcgca acctcctgga caacgcgctc aactacacac ccgagggcgg gcaggtcacg
     1261 gtgcgcctgc tgaccgaccg cttcagcggc gtggtggtgc tgcaggtcga ggactcgggc
     1321 ccgggcatcc cggccgccga gcgcgagctg gtgctgcagc cgttttaccg cgcgctcggc
     1381 accaatgtcg acggttcggg actggggctc gccatcgcgg aggaaatcgc caaacagcat
     1441 ggcgccaccg tcgcggttga tgacgccgtg cccggccaca cgccgccggg gacacgcatc
     1501 accgttcggt tcatggggtt tgccgacctg ccgcaggaat aaaggccggc acctcactac
     1561 actgggcact gcccccgaac cgacgacctg cgactggatc cgcgatgcgc aaaacgacag
     1621 ccatgaacag ccagcgccac atgcgcaccc ggctcaaaat cggcctctcg gcctgctttt
     1681 cccacgccga cccgacgcgt tcgctgttca ccggcaaaac cctgcaatac gtcgagcaga
     1741 cggtcgccca ctggatcatg tcctcgggcg cgatggtggt catggtgccc tgtccgaccg
     1801 gcgccaccca gcgcggcgat gcgggtttcg acgactatgc ccagtggctc gacggcctgg
     1861 tgctgcacgg cggcgccgat gtctggcccg gcagctacgg cgaagagccg ctcgaagaac
     1921 gctggtccgg cgaccgcgtg cgcgacgact acgacaaggc catggtggcg tctttcgaaa
     1981 aagccggcaa gccggtgttc ggcatctgcc gcggcctgca gctgctcaat gtggccttcg
     2041 gcggcacgct gtaccaggac atcgccaccc aggtgcccga gtccttcctg catcgcgacg
     2101 cggtggtcta cgaccagaac ttccacagcg tggagatcgt gcagggcacg cagttgtccc
     2161 gcctttaccc tggcgtggaa cgcgcacgtg tcaacagcat tcaccatcaa ggcatcaaga
     2221 agctggcgcc cgagttcgtg gccgaagcct tcagcgtgcc cgacggcatg gtggaagcca
     2281 tacgccgcaa ggacccggcc aaaagctaca tcgcggcgct gcagtggcac cccgagtttc
     2341 accagccggg ttcggacacg attgacgatg cggcggtact ggaggatttt ctttccgcgg
     2401 tggcggcggc gaagaagcac gctacggcct gacatgccgc cggctgcgtg ccgaacaagc
     2461 ctccagccct tactggacaa gcgcaaacag ctacctaatt tatagcaaaa tttcctgaca
     2521 cgggtccatt ccggacttga tccgagatcc atgtttgccg gctaccgact gtgtttttga
     2581 ccgtgtgtgg ttcgcctgca ggccgggtct cgccccggcg ggcgaggcac tttacttttg
     2641 cgtcgccaaa agaaagtacc caaagaaaag gcgacccgat ggtctgggtc cttccgct
//
LOCUS       DAIDFG010000378         2690 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_378_length_2690_cov_21.445920, whole genome shotgun sequence.
ACCESSION   DAIDFG010000378 DAIDFG010000000
VERSION     DAIDFG010000378.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_378_length_2690_cov_21.445920"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          478 a          928 c          828 g          456 t
ORIGIN      
        1 accgccactt cggcttcgaa gtcgatgccg tagtcctcgc tggggcagac cacgtcgtcg
       61 caggggccga tgaagtcgtc gctgccgccc tggtacatca gcgggtcctt gtagaaactg
      121 tcgggcacgt cggagtcgcg tgccttgcgc accagctcca catggttgat gtaggccgag
      181 ccgtcggccc actggtaggc gcgcggcagc ggcgccatgc agcgcgccgg ctcgaacgga
      241 aacgcgtggc gtgccttgcc gttgttcagc gtctcgtaca ggtcctgcaa ttgcggggcg
      301 aggaagttcc agtcgtcgag cacctgctgc aatttgctgg cgatgcccgt cgcataatgg
      361 gccgtcgaca ggtcgcgcga gacaaccacg agctggccgt cacgtgagcc gtctttgtag
      421 gtagcgagtt tcatcagttt cctctgttct gggggccgga tgggtgaagc tgattgcacc
      481 ctgcgtacac tctgcgctag agttacgata agtgaaatca atttacctaa atgtaatttt
      541 taattttagt cgaattccat gaaagagcgc aaaacgccga ccgaaaactc gccaggcagc
      601 ctgcccgccc agcgtgcagg catccagtct gtagaggtcg gcttcagcct gctgcaggtg
      661 ctgggcgcgt caccgggcgc gctgatgctg cgcgacctgg ccgctgcagc cggcatgagt
      721 gccgccaagg cgcaccgcta cctcgtgagt ttccagcggc tagagctggt gacccaggat
      781 ccgggcagca cacggtatga ccttgggcca gcggccctca agctcggcct ggcttcactt
      841 tcacggttgg acagcgtcaa gctcgcacgc cagcgcctcg cgccgctgat ggagcagatt
      901 ggccaaaccc tggcgctggc ggtgtggggc aaccaggggc ccaccatcgt gcactgggaa
      961 gaatcgccac aggccgtcac cgtcaatttg cgcctcggcg acgtgatgcc tttgctgtcc
     1021 tcggccaccg gcctgtgttt ctccgccttc atggcccagc cgtctgccgg gcggcggcac
     1081 ccccacgacc gtcttgcccc cttgctcaag aacgaactgg cacgcatgca caagctcgcg
     1141 cgcaaggatg ttccccaaac catggccgag gtcacggcgg tactggaaga ggtgcgccgg
     1201 cgtggcctgg cccgcgtggt ggacaccttg ctgcccggag tggccggatt ctgcgcgccg
     1261 gtgttcgacg ccgacgggca cctggtgctg ggcgtggtgg cgatgggctc gctggccagc
     1321 ttcgacccgg cctgggacgg cgccgtagcc cggccgctgg cagctgccgc acggcaactt
     1381 tccgctgatc tgggattcac aggggcatga acaccccgac cgcccggcag gccccagcgc
     1441 tgccggcgcg acccctgatc gccctgaccg cgctggtgat cctggcccat gtcctgctgc
     1501 tgcaggcggc gccgctggca tgggggccgg tgcccgaccc gcaggcgacc gcgccacgcc
     1561 ccttcatcac gcgcacgctg gagatcaagc cggaaccggt cgtgaccagt gcgcccgcgg
     1621 ccacgccggt gcgtccgcgc ccggtccggc gtcgcgcccc tgtttcgcaa gcaaatcggg
     1681 caccagcccc cgtttcgaca gcgccagcag ctatcgaatc gatagcgcca tcagccgatg
     1741 acagcacaag tccggtcgac ccccagaccc cgccaccaga ggtcaccgca accaccaccg
     1801 aaccggtcgc gcccacgccc gtggaagcag cgccgcctcc agacgccagc acggccctga
     1861 ccatccccgg ctcggtgcgc ctgaaatacg tgatgacggg gcgatcaaaa aacatgaact
     1921 acagcgcctt tgcccagctc gactggctgc aggacggcca gacctatgac gccaagatgg
     1981 tggtcagcgc cctgttcctg ggatcgcgca gcatgaccag cagcggccgc atcaccgccg
     2041 acggcctggc gccaacgcgt tttctggaca agtcgcgcag cgaacgcgcg gcgcacttcg
     2101 aggaagacaa gggcaagatc agcttcagtg ccaacacgcc tgacgcccct tggctgcgcg
     2161 gcgcgcagga ccgcctcagt gttttcctgc aactgggcag cctgctcgcc ggcgccccgg
     2221 ccagctaccc ggtcggcagc agcgtgtcgc tttacaccgc cggcccgcgc gacgccgaca
     2281 cctggacgtt cacggtcgag tccgaggaaa ccctccagtt gcccgccggc gaggctgcca
     2341 cgctcaagct cacgcgcaag ccgcagcgcg actacgacca gaccgtcgaa atctggttcg
     2401 cgccctccat ggactacctg ccggtgcgca gccgcatcac ccagcagaac ggcgacttcg
     2461 tcgaccagca actgcgcgtg gtcgagaaac cctgacatcc gcaggcgcca gcgcgtctct
     2521 ttccttcacg atgtgatttt tttggcattt cagggattaa ccctgtggct cttgaactcc
     2581 gcccagccgt tgatatatag atacaagaaa agaaggaaac cacaccatgc aaatgctcta
     2641 cgactccgaa tcctttgcgg tggtgcagat tcatgccaac gccccagagg
//
LOCUS       DAIDFG010000379         2669 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_379_length_2669_cov_15.424637, whole genome shotgun sequence.
ACCESSION   DAIDFG010000379 DAIDFG010000000
VERSION     DAIDFG010000379.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_379_length_2669_cov_15.424637"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          420 a          863 c          941 g          445 t
ORIGIN      
        1 cgggacgcca ggggcgcgct caggggccgc gctacccgcg cctcttgtcc ctgcgtggta
       61 cccggtctgt aacccgcgcc tttccaaagg tggggtaaac aagtacgatg agcagcgtaa
      121 taaagttacc gactcatctt cagccacctg cttggcccac accccccatg acccttcatt
      181 ccaccgtcgc cgacgtcacc gcgcgcattc gcgagcgcag ccgggcgacg cgcagcgcct
      241 acctccagcg cctggacggc gcggccaccc gggccatcag tgttgaccgc atgggttgcg
      301 gcaacatcgc gcacgcggtg gccggcatgc cgctggacga ccgcttcaag gtggtcacgc
      361 agcgcgcgcc caacatcggc atcgtgaccg cctacaacga catgttgtcg gcgcacacgc
      421 cgctgcagcc gtatccggac ctgatcaagt gggaagcgcg cctgcatggc gccaccgccc
      481 aggtcgccgg cggtgtgccg gccatgtgtg acggtgtcac gcagggcacg ccgggcatgg
      541 agctcagcct gttcagccgc gatgtgattg ccatggcaac cgccgtctcc ctgagccatg
      601 acatgttcga tgcggcgctg atgctgggcg tgtgcgacaa gatcgtgccg ggcctgctga
      661 taggcgcgct gcatttcggc cacctgccca tggtgtttgt gccggccggc cccatgccgt
      721 ccggcctgcc caacaaggaa aaagcccgcg tgcgcgagca ggccgcgcag ggtctggtcg
      781 ggcgcgcgga attgctcgag gcggagctca agagttacca cagccccggc acctgcacct
      841 tttacggcac ggccaacagc aatcagatgc tgcttgaagc catgggcctg cacgtgcccg
      901 gcactgcgtt tgtgaacccc ggcggcgacc tgcgcgacga actcacgcgc gaggcggcgc
      961 gtaccgtgtt gggcatcgtg aaatcctcga aacgtttttc gcccatcggg aaaatcgtcg
     1021 atgaaaaggc catcgtcaac gccatggtcg cgctgctggc caccggcggc tccaccaatc
     1081 acctgatcca ctgggtggca gtggcgcgtt cggccggcat cctgatcgac tgggacgatt
     1141 tttccgcctt gtccgcggtg gtgccgctgt tgacccatgt ctatcccaac ggcagcgccg
     1201 acgtgaacca gttccaggcg gccggcggca cgggtttcgt gatccgtgaa ctgctcgatg
     1261 ccggcttcat gcacgaggac gtgcttaccg tgcgcgcagg tggcatccgc gaatacaccc
     1321 gtgagccggc catggacggc gacaagctgg cctggcacga gatgggcgcg acgaaagacg
     1381 aggccatcgt gcggccggcc agcgcgcctt tcagcgccac cggcggcctc aagctgctgc
     1441 agggcaacct gggccgcagc gtgatcaagg tctcggccgt gcccgacgac cgccatgtca
     1501 tcgaggcgcc ggcacgcgtg tttgattcgc aggaggacct gcatgtggct ttccgtgccg
     1561 gtgaactgga ccgcgacgtg gtctgtgtgg tgcgctggca gggcccgcag gccaacggca
     1621 tgcccgagct gcacaaactc acgccaccgc tggcggtgtt gcagggcaag gggttcaagg
     1681 tggcgctggt gaccgatggg cgcatgagcg gcgcctcggg caaggtgccg gcggccatcc
     1741 atgtgtcacc ggaagcggcg gccggcggcc cgctggcgaa agtgcgtgat ggcgatgtgg
     1801 tgcgagtcga cgcgttcacc ggcacgctgg aggtcaggat cacagccgac gaatgggcgg
     1861 cgcggccgac cgcggtcatg ccgcaggcgc tgcgtgatgc caatggtcag ggtctggggc
     1921 gcgaactgtt tgccggcatg cgccggcacg cgctgacggc cgaagaagga gcgttatcgt
     1981 ggatgtgagt atcgatatcc agggcccgct gacggccctg caggtgatgc aggatgcggc
     2041 ggtcattccg gtgattgtgc tgaccgatgt ggcgcacgcc gtgccgctgg cgcgagcgct
     2101 ggtggctggc gggatacgca tgctggaagt gaccctgcgc acgccgcagg cgctggcctg
     2161 catcgaagcg attgcgcgtg atgtgcccga agccgtggtc ggcgccggca cggcgcgcag
     2221 tgcggccgac gtgcaggcct gcgcgatggc cggcgcgcgt tttatcgtca gcccgggcta
     2281 cacgcatgcg gtgggcgcgg cctgccgcga tgcgcacctg ccgctgttgc ccggcgtggc
     2341 cacgggcagc gaaatcatgg cggcgcagga agacgggttc accgaactca agtttttccc
     2401 ggccctgcag gccggcgggc tggccatgct caaggcctgg agcggcccgt ttggtgacgt
     2461 gacgttctgt cccaccggcg gcatcacggc agcgaacgcg gccgagtttc tggcactgcc
     2521 caacgtggtg tgtgtcggcg gctcctggct ggtgccggcc gatgcgctgg cgcgcggcga
     2581 ttggggccgc gtgacggagc tggcccggca cgccagtgag ttgccgcggc aagcgggttg
     2641 atttcgactc gagaagaatc gtgtgttgg
//
LOCUS       DAIDFG010000380         2637 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_380_length_2637_cov_17.480248, whole genome shotgun sequence.
ACCESSION   DAIDFG010000380 DAIDFG010000000
VERSION     DAIDFG010000380.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_380_length_2637_cov_17.480248"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          499 a          861 c          818 g          459 t
ORIGIN      
        1 cggcgcgcac catgtcctga acttgctgat tccctacctc gagcagggcc agcgcatgca
       61 cctgcaggcc ctggtgggtg cctacaccaa ccccgcctga tcgccgattt tccagccagc
      121 caactataaa tttcaggaga cgaccaatgg ccaaatggac tgccttcccc cacgccggcg
      181 actacgcatt cgatgccgcc agcctgaaga aaaactgggc ccgcctgcac cagggggact
      241 gcgagcccct gcccaaggac gccgccgcgc tgcaggcctg ggtgctgttc cacaacggcg
      301 acttccagaa ggccgccgaa gcgggcctga aggcaggcgg cgccggcatc accgtggcca
      361 acaaggccac gtccatttac gccaactacc tcgagaacag ggaaaagacc aagctcgagc
      421 tgttcatgga agtcgcccag cgcgccgagg cccagcaggc cgccgagccc cagaacgcca
      481 acgcctggta ctggcaggcg tacgccctcg ggcgctacag ccagggcatc agcgtggcca
      541 aggcgctcgc ccagggcctg ggcagcaagg tcaagaacgc gctggagcag gccatcaggc
      601 tctgccccaa gcatgccgac gcgcacattg cgctggccgc ctttcacgcc gaggtgattg
      661 acaaggtcgg ctccctgatc ggcggcatga cctacggcgc caagaaggac gtgggcctgt
      721 ccctgttcaa ggagtccctc aagctcaacc caggctcggc catcgccatg atcgaatacg
      781 ccaacggcat ggtgatgctc gaaggcgaca agcggatgaa agaggccacc aagctctacg
      841 aacaggccgc tgccagcaca ccgatggacg cgatggagcg gctcgatgtg gaaatggcca
      901 aggccgaact ggaagactga gctacaaaaa aaagcgcatc cgctatattt tttatagctg
      961 aatgcgcccg tccatcaagg gttcgcgccc aaaaagacta tgaattcagc ccatccaccg
     1021 cctggaacag cgactcccgt gcctggctga tgatctgttc tttccaggcg tcggtgtcca
     1081 cgtaaaacgc atccatcatc tgctgcttga tctgcgcggc cagtgccggt ggcgcatcgc
     1141 ggtggacatt gagccagtgt tcggcacgca gcgcctgcat catctcgaag ggcggcaaag
     1201 tgccgtactc catcgcgatg ccggtgtatt ccgcctcggg gcactcgtcg tagatcgagg
     1261 tccacatcag gcccgtcagg aaagccgaac tcgacgagcc gtcgtaaatc gaggtcaccg
     1321 gtgtcgcacc accgccgccc caccaggcgc gtgcccgggc cagcgcttcc ttgtcatcgc
     1381 gacaggcgca gatgcgttcg ccgacaccgc tgggacccag tccggtgtga acgtcaatcc
     1441 atgccacccg cctggcctgg cgcgcatggg tctgcagcac cttgcgcagg gtctggttgc
     1501 tccaggtcgg cgcggtgccg ccgaaaaaca ggccctcggg gaattcatgc tggccccggg
     1561 tgatggctgc ctggtaggca tccatgccgc ggctggcgat gtaatggtcg gtggccacct
     1621 tgttgtcgtc gttgggcggc cactgctcgg gcagcaacag cggctgcagc tcgcggtaag
     1681 cctcgttgac aggcaaggtc tgcgagaagt catggaagtt gcggttcaga tccacgtttt
     1741 catgggtggt gcgccggatg tgcgaaaagc cataggggtt gagcgcatgc acgtacagca
     1801 ccgcaacgcc ctgcgccctg gccttggcgc gccactcggc gtcccgcagg gcaaacacct
     1861 gcacgccgga gccgcaataa ccttcgaccc catggcaggc gctggtgagg atcagcagcg
     1921 aacgggcgtc ggccgggccg tccagcacca cgtccatggc cagctcttcg ccctcgcgcc
     1981 cggcttcagg gtgaatgtgc gaatccgcct gcaggccggc cgcagcggcg gcttcgagga
     2041 atttgccgcg cgcttcggca taggtgccgg aaaaagcttc ggtcactccg ctcatgctgc
     2101 gtccttggcc agccagcttt cgctcaggcg cacccagaag gtggccccca gcgggatcag
     2161 gtcgtcgttg aagtcataac tcgggttgtg cagcatgcac ggccccccgc cgtggcccat
     2221 ctcgcggtgt gcgccgtcgc cgttggcaat aaagacgtag cagcccggtt tggcctgcag
     2281 catgtaggaa aagtcttccg cgcccatggt cggctcctgg gcaatcacgt tggcctcgcc
     2341 gacgatctcg gccatcacct tgcgcgcaaa ctcggtttcc ttggcatggt tgatggtggg
     2401 cgggtagttg cgcacaaacg aaaattcgca ctgggcgtca tgcgccgcgc agaggtgctc
     2461 ggcgatctgc tgcatgcgtt tttctatcag gtccagcact tccagcgtga aggtgcgcac
     2521 cgtgccctgc agctcgcagc tgtcggggat cacgttggtg gcctcgccgg catggatcat
     2581 ggtgaccgag atcacaccgg catcgaccgg cttcttgttg cggctgatga tggtctg
//
LOCUS       DAIDFG010000381         2619 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_381_length_2619_cov_14.607644, whole genome shotgun sequence.
ACCESSION   DAIDFG010000381 DAIDFG010000000
VERSION     DAIDFG010000381.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_381_length_2619_cov_14.607644"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          485 a          804 c          798 g          532 t
ORIGIN      
        1 tgcagcgccc gctcgatgga cgacacgccg atgtcgtaga agtcgaggcg gtcccggttg
       61 cggaccacca gggtatccac aaaaaggtgc cgctttgcga tggcttccag gaccgctgcc
      121 ggcgctggtg atgggagggt tttggctggt ttgttcatgg acccagtaac gctctcttcg
      181 ggccggccag caagcggttt tgccggcagt tcggcactag tgcgcacctt gcttgaaatt
      241 gctgatgatc agctcgcctt tgggttcccg gccgcgccct gaagcgccga ccgagtagct
      301 gatgctgacc cgcttcatgc tgtgcccctt gaacgccttg cgcatctcgg ggatgtcgtt
      361 cacgctgacc accgccttgc ccttcatggt gcccatcagc tcggccatca ggtcgtactg
      421 ttcaagccca aacgccacgc cgtagccctc ggtgccccag tagggtggat ccaaataaaa
      481 aaggctgtgg gggcggtcgt agcgggccac gcaggccgac cactccaggc gctcgataaa
      541 cacctggtgc agccggaggt ggacggcgct gagctgctct tccagccgca gcagattcag
      601 cctgccgggg ctggtggtcg ccgtgccgaa ggtctggcct tgcaccttgc cgccgaagcc
      661 gtttttctga aggtaaaaaa accgtgcagc gcgctggatg tcggtcagcg tatcgggcgg
      721 ggtgatctgc agccacttgt aaatctcgcg gctggtcaag gcccatttaa agtgccttac
      781 aaactcctcc aggtgatgct ggaccacccg gtaaagggtg accagatcac cgttgatatc
      841 gttgagcacc tctaccttga ccggcgcctt caaaaagaag agcgctgcgg caccgcagaa
      901 gggctcaacg tagcaggaat gatccggaaa cagcggaagg atgtgcgggg ccaggcggcg
      961 cttgccgccc acccactgca ccatcggtgc cgcctggacc gtgtcctgct cgcctgaatt
     1021 gttaaagagc tttggccgct cccgttttgt cgtcatcttt ggttccgttt tcagatgacg
     1081 ctcggcggcg tgctggtgtg gtgctcgggc tgctgtgatc agctgcgctg gcactcaagt
     1141 cattcagcgt tttgcaacgc tggcatttaa ttctcagcct ggtgaacaag ccttcgccga
     1201 gctttcgctc gcattggccg cacctgatgt ctttcagttc ttgtcgcatg gcatcgccgt
     1261 tatgatggcc cggcctgtac aggtggcagg gtcttcggtc aataccgtgc tcgctcacgg
     1321 tggaggcggg ggcttgaggt gttagtgcac cgcaggcctt cgccctgtct ttttttaagc
     1381 cggggccagc acctcctggt gtgcataggt caacgcggca tggaaggaat tggggccaaa
     1441 gcgccagggc tctggcaagc ctagcgaagc gccgactgat tcattgcaaa accagtggtc
     1501 tggtcgctcg gcccaaggtc cccaaacaaa acccaccaag ccggccacgt catatttctc
     1561 gcctaggtgt tcaacgaacc aacgcttggc tgcgcaggcc tgctgcaacg tgacctcgat
     1621 ggtgaccaga tcccaatggg ctgggtccag cacgatcagc ttgcggcgca cgccgccatc
     1681 gatgaacgat gacgaatagc agatcgcctt gtcgccctcg tagcccgtga tcagctcgca
     1741 gtgcgaatac ggaccgcgct cccaccagga cactgcgcgg ttgaatagcc ggccggtgcc
     1801 tttgtagaac gcgacctgta gctgcatgga ttcagagagt tgcggcgatc accatcgctt
     1861 gagcgacgat gtcgtcatgc agtgcctggg ctgcagccgc gtcagcagcg gcggcgatgc
     1921 cgtatttacg catgcgctgg gcggcgagca gctccagagc gcctcgcagt tggtcggcct
     1981 gctggatgat cagatcggca gcgacctggc tggtcagacc ggcatttgtc gcaaacgcag
     2041 tcacccacga acccgcatcc ccggcatagc cgcccgcctt gaaggcgcgc gcagcatcct
     2101 cgcgcttcgt gtactccgac tcgaagcgcg tgaagcggct ataaataccg ctgatcgcgc
     2161 cgtctacaag agccaccagt tcggccttga catccgccag ggcgggcggc ggcttggctg
     2221 gcgtcgagcc ttcaggcggc gccacgccaa gcgcttcgac ccgaccttcg acaccgttca
     2281 gccagtaaga cacgccccga tagttcgggg tgatggccca ctcgccattg acaaaaaccg
     2341 ccgcttcatg cgcgccagta gctggaggcg gtagcgaggc ggaacacacc ggtgtgataa
     2401 acgtgcctgg ctccagcggg ctttcctggg cgtcataccg gccagcgaac tcgcctgtgt
     2461 ttttgtcgta caaataaact gtttttgaag tcatgacata gccttaaaac tgaacgcaca
     2521 tcagcacgcg agcacctgcg gcatagttgg cggcaccacc ggtacttgtc acggtcccgg
     2581 gcgatggaat ttcagaggtg cccggaccgg tgccgccgt
//
LOCUS       DAIDFG010000382         2611 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_382_length_2611_cov_16.049296, whole genome shotgun sequence.
ACCESSION   DAIDFG010000382 DAIDFG010000000
VERSION     DAIDFG010000382.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_382_length_2611_cov_16.049296"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          403 a          922 c          843 g          443 t
ORIGIN      
        1 ctccgagcgt tttgtgcgcc acctgaacac gctgcgcaaa tcatgaatca ggcccccacg
       61 ctcgttgctt cgcgtacttc gctgcccccc gggggggcgc attttttctt ggggcggccc
      121 ggcgaaaaaa ccccgaccac caagcccctc aggagcgcgg catgaatttc cgccgttcat
      181 cgcgcgacga gcctgagatc aacctgattc ccttcatcga cgtgctgctg gtcgtgctga
      241 tcttcctgat gctgtcgacc acctacagca agttcaccga gctacagatc aagctgccgg
      301 tggccgacgc cgaccagcag cgcgactacc ccaaggaagt gatcgtcgcc gtcagcagcg
      361 acggccgcta catggtcagc aaggtgccgg ttcccggccg cagcatcgag gcgctgagtg
      421 ccgccatcag cgcggcggcc aaggccggca aggacagcgt gatcatcatc agcgccgatg
      481 ccagcgccac gcaccagtcg gtcatcaccg tgatggaagc ggcccggcgc agcggcctgg
      541 cccagatcac ctttgccacc cagagcgcgg cgcaggccgg cgcccggtaa acgtgacgac
      601 ccggctgcag caggcctggc tgcggcgcgg catgctcgcc tgcctgctct ggccgctgtc
      661 ccggctgtac ggcctgctgg ccgagttgcg caggctggcc ttccgggccg gctacttcag
      721 atctgaacgc ctgcccttgc cggtggtggt ggtgggcaac gtcgtggcgg gcggtgccgg
      781 caaaaccccg gcggtgattg ccctggtgca gcacctgcag gcccgcggtt tgcaggtcgg
      841 ggtggtctcg cgcggctacg ggcgcaccag tgcgcattgc ctggaggtct tgccgggcac
      901 ccccatcgac gagtcaggcg acgagccggc cctgatccgg cgggcgacag gcgctcctgt
      961 ttttgtagca tcccggcgca ccgaggccgc acgcgcgctg ctcgctgcac accgcgcgac
     1021 acaggtgatc gtctgcgacg acggcctgca gcattacgcg ctgcagcgtg atgtcgagat
     1081 tgcggtgttc gacgaacgcg gcgtcggcaa tggctggctg ctgccggccg gccccctgcg
     1141 cgaaccctgg ccggagcgcc ggcaacgcgg cgtacgcgcc ggcgggcgcg gtatcgacct
     1201 ggtgctgcac accggcggcc acaccgcctt gacgggtttt tcaggggttc cgggttttgg
     1261 ttcgacgcgg cgcctggctg gccacgcgct gcgcgccgac ggcagccgcg tggctctgga
     1321 gagcctggcc ggccggccgc tgctggcgct ggccggcatc gccaaacccg aggctttttt
     1381 tgccatgctg cgggcccgcg gcctggtact ggccgaaacc cgggcattgc cagatcacta
     1441 cagttttgat agctatccgc gcccgcagga caagggctac accgttattt gcactgaaaa
     1501 agacgcggtc aagcttttcc cccggcaagc ggacgtgctg gccgtgccgc tggagttttc
     1561 acccgaacct gccttcttca cggcgctcga cgccttgctg gcccccctgc tttctccatt
     1621 accatcctcc catggacacc aaactgcttg aactgctggt ctgccccgtg accaagggcc
     1681 cgctcgacta cgaccgtgaa aaacaggagc tgatctcgcg cagcgcacgt ctggcctacc
     1741 ccatccgcga cggcctgccg gtgatgctcg agagcgaggc acgcaccctc acagacgagg
     1801 aactcggcct gtgacctgcc gcgcatgagt tttaccgtcc tcattccggc ccgcctcgcg
     1861 tccacccgcc tgcccgacaa accgctggcc gacatcgcgg gcgtgcccat ggtggtccgc
     1921 gtggcgcagc gcgccttgct cagcggcgca tcgcgcgtcg tggtggccac cgacagccca
     1981 cgcattgccg aaagctgcgc ggccttccag gtcacgacgg ttttaaccca cgccgatcac
     2041 cccagcggca gcgaccgcct ggccgaagcc tgcaatctgc tgcggctcgg caacgacgaa
     2101 gtggtggtca atgtgcaggg cgacgagccg ctgattgatc ccgcgctgat tgacgcggtg
     2161 gcgggtttac tcgccgatcg gcccgactgc gccatgagta ccgccgcgca cgccatcaac
     2221 tcgatcacag acctgaacaa cccgaacgtg gtcaaggtgg tgctggatgc gcgcaatact
     2281 gcgctgtact tcagccgtgc gcccataccc gcagcgcgtg atgccggcgg tcaggcatgg
     2341 tggggtgccg gcagtcagtt gccgccgccc ctgcggcacg tgggcatcta tggctaccgc
     2401 gtcggtttcc tgcggcagtt tccggccttg ccgcaagcac cgctggagcg gctcgagcaa
     2461 ctcgagcaac tgcgcgcgct ctggcacggc caccgcattg ccgtccacgt caccgcgcag
     2521 gcgcccgggc ccggcgtcga tacccccgag gacctggagc gcgtacgacg gctgtttgtg
     2581 taagctgcgc gcggggggac gcatgctatc c
//
LOCUS       DAIDFG010000383         2611 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_383_length_2611_cov_10.368153, whole genome shotgun sequence.
ACCESSION   DAIDFG010000383 DAIDFG010000000
VERSION     DAIDFG010000383.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_383_length_2611_cov_10.368153"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          461 a          848 c          809 g          493 t
ORIGIN      
        1 gggcgaggtg ctggccctcc taggcccctc cggctgcggc aagaccacgc tgctccggct
       61 ggttgccggg ctggaagttc cggatgccgg cagccgcatc agctttggcg acaccgaggt
      121 cacgcatttg ccggtggagc agcgcggcat cggcatggtg ttccagcact acgcgctgtt
      181 tccgcagatg acggtcgaag ccaacatcgg ttatggcctg cgcgtacggg gtaccgcgga
      241 ggccgagaag cgccgtgtgg tcggtgagct gatcgacctg gttcgtctca acggccttga
      301 aaagaagcgc ccggccgaac tctcgggcgg gcagcgccaa cgcgtggccc tggctcgcgc
      361 ggtagcagtg cgcccgcgtg tactgctact cgacgaaccc ctgaccgcgc ttgacgccaa
      421 gctcaaggaa tcggtgcggg atgaactggc cgagctgctg cgccgcctgc acattaccgc
      481 catccacgtc acgcatgatc agcaggaggc gctggccatt gccgacaggc tggccgtgat
      541 gcaagccggc cgcatcgtgc aggttggcga tggcgaggcg ctgtaccgta cgccccagca
      601 tccttttgtt gcgagctttc tgggccgcgt caaccgcctg gcgcgcgacg aagttgcgcg
      661 cgcagccaac gtcgtaaagg tcggcagcat ggccgtcgcc tgcccgccgg cctggagcgc
      721 acaagacaca gtactcgtcc ggccggagga cattgaagtg gccgacatca catccggctg
      781 gtgcgacgcc atggtggcgc agcgcgtttt cctcggcgac cgcatccagc tcaagctgat
      841 gctgcctgac cagaccagcg tcaacgcgga agtcgggcgc gatcactgcg cccgcatggg
      901 cgatgccgtc ggcatccgca ttcaatccca ccacctcatg cccggcagcg aactgactgc
      961 caccccctca tgactacctc attgctgcaa ctctccgacc cgcatatccg cgaaccgggg
     1021 cgcctggcct acgcccgcat caacaccgcg ccctacctgg cgcaagcggt cgcgtccatc
     1081 ctcaggctgc cgcagcagcc ggatgcggtg gtcattaccg gcgatttgac cgactttggc
     1141 cgcgccgctg aatacgagca tttgcgtgag ctgctcgcgc cgctggccat gccggtgtac
     1201 ctgatgcctg gcaaccacga tgaccgtgcc cagctacgtc gaagctttcc tggccacacc
     1261 tatctgggtc acgatgaatt tgtgcagtac agcgttgccc tgggcgcgct gcagatgatc
     1321 gccctggaca ccgtggtgca cggcgcgagt gaaggcagcc tgtgcgagga aagactggcc
     1381 tggctggcca gcgaacttga agcgcaccgc agccggcccg tggtaatcgc catgcaccat
     1441 ccaccattca agaccttgat cggccatatg gacgacattg gcctgctcag cggagcggcc
     1501 gaactggaag cgctggtgag aaaatactcc aacgtcgagc gcatcatctg cggccacctg
     1561 caccgggcca ttcaggtgcg ttttggtggc accatcgccg cgacggtgcc ctcaccggcc
     1621 caccaggtct gccttgacct ggcgcccgac gccgcctcag catgggtgct ggagccaccc
     1681 ggatttgggc tgcacgtcct gcccgatggt ggccgcctgg tcagccacat agtggcaagt
     1741 ggcccgtatg aaggcccgta tccttttcac gacggggcgg tgctgattga ctagtaaaga
     1801 cacctgaccg tattgccgct aatctgcaaa aacatgcgcc ggctatttcc gaggatcaat
     1861 atgtatttcc tcgcacagga caccagcacc aagtcgatgc gttgagtgca gttcgctctc
     1921 gctgtccaaa gcctgaagta aagtccgccg tggtacttcg gtgttggatt tcttgttccc
     1981 attgttgaat tctgtcgaaa tctgatccac ttatcgcagc catgcatgca tacggtgcag
     2041 tttgccctcg cgcatattgg gcaatcctcg cacttgtccc gtgaagacgc cgtttaccag
     2101 ctccaactcc gtcgacaacc agttcgtgaa gcaagttcgg cagcgctgcg ctcggcgatc
     2161 acgctgttgc tggcggtcgt gagtaccagc acatggcctg ccgcacggtg cgacgtcacc
     2221 agggcgcgcg cagcctctgg aagtcgatgt cggatctctt cggccacaaa cctggcctgc
     2281 caaggcggcc attcggctgg tgtgcggccg gccagcaggc tggcgtagaa gtcgctgaac
     2341 gcttcgacgc tgacccggcc cgcgcggtag tctgcgccca tttgttcgtt ctggttctgc
     2401 atccctgcat ctacgaggcc gtggcgcaag agaaagcggc accacaggtc atcgccgtcg
     2461 ccatcgagca aggtgtggtc gaggtcaaac aaggccagtg tggtcatggg ctggggtggc
     2521 atggcttgcg ctgttcaatc tggatatcgg ttgtcatgcg tccgcaggcc tgcgcgacgc
     2581 gtgccggcag gcggcaccag cgccggtatc a
//
LOCUS       DAIDFG010000384         2582 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_384_length_2582_cov_19.068461, whole genome shotgun sequence.
ACCESSION   DAIDFG010000384 DAIDFG010000000
VERSION     DAIDFG010000384.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_384_length_2582_cov_19.068461"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          469 a          856 c          801 g          456 t
ORIGIN      
        1 agggggccgc tgcgcctgcg ggccggcgaa gccggacccg cggcccctgc ttgaaaagac
       61 atcgccgcgc gatcgctcac tgaataccgt tcgtcctgag cttgtcgaag gatgccctcc
      121 cgacctgaga caatacaagc atgtttgctc ccctccaaaa cgacaccttt ttacgcgcct
      181 gcctgcgtca ggccaccgat tacacccccg tctggctgat gcgccaggcc ggacgctacc
      241 tgccggagta ccgcgccagc cgcgccaagg ccggcagttt catgggcctg gcgacctcgc
      301 ccgactacgc caccgaagtc acgctgcagc cgctggagcg ttacccgctg gacgcggcca
      361 tcctgttttc cgacatcctg accgtgcccg acgccatggg cctgggcctg tcgtttgcgc
      421 tcggcgaagg tccgcgtttt gccaccccgg tgcgtgacga agcggccgtg gcaaaactcg
      481 aagtgccgga catggaaaaa ctgcgttatg tctttgacgc cgtcacctcc atccgcaagg
      541 cgctgaacgg ccgggtgccg ctgatcggtt tttccggcag cccctggacg ctggcctgct
      601 acatggtcga aggcgccggc tcggacgact accggctggt caagaccatg ctgtaccagc
      661 gccccgacct gatgcacagg atgctcgcca tcaatgccga tgcggtggcg gcctacctga
      721 acgcccagat cgaggccggc gcgcaggcag tgatgatttt cgacagctgg ggcggcgtac
      781 tggccgacgc ggccttccag acctttagcc tggcctacac ccagcgcgtg ctgagccagc
      841 tcaagcgtag ccatgacggc gtggtcattc cgcgcctggt gttcaccaag ggcggcggcc
      901 tgtggctgga acagatggcc ggactcgact gcgaggtgct cggcctggac tggaccgtga
      961 acctggccca ggcccgggca cgcgtgggcg atcgcaaggc gctgcagggc aacctggacc
     1021 cgaacgtgct gtttgcccag ccggcgcaga tcgagcagga agtggccgcg gtcctggaga
     1081 gtttcggtac cccgcatgcg gacacgcgca gcagcggccc gacccacatc ttcaacctgg
     1141 gccacggcat cagccagcac accccgcccg aaaacgtgga ggtgctggtc cgcgccgtgc
     1201 atgcctattc gcgccgcatg cgcacgggta ccccgcattg atgcgggttt tgccgtctga
     1261 tagcacaaat tccagctccg cagacccgac ttattcacaa aatttgtgct gcggtgccgc
     1321 atgaagctgt aacaagtcgg tctcccgcta tcaaagccat agcgcttcta agtgcttgat
     1381 tcatatagga tctttaaact gctttttttg cgggcaattt aagaaaagcc ttgatttata
     1441 aggcccggac gcgtgccggg gcaatctatc aacaaagtta tccacagaaa ccttggaaga
     1501 ccgtcaaagc ttttttaaat caagcactta cagcgatgtt cgcaaatgta tctgaggact
     1561 atctgcttaa ctatcccctc tgttcttcca atcgatccac ccgaaaatga ggtattggct
     1621 cagcatcgtt gtcgccacac ccgcccacag cagcgtggcc ggaccgctga cctatcggag
     1681 cgagtggccg cttccgccgg gaagcctggt gcgcgttccg ctgggcaaac gcgaggtcct
     1741 gggcgtggtg tgggaggtct tgccggacag cggcgacctg ccggaaagcc agtccaaagc
     1801 ggtgctgggc gcgctggacg gcctggcgcc cctgtcggcc acctggcggc ggctggtcgg
     1861 tttcaccgcc agctactacc agcgttccat tggcgaagtg gccctggccg ccctgccgcc
     1921 gcaattgcgc gaactgagca ccgtgcagct ggcacgccgg ctcaagcggc cggtggcccc
     1981 cgcgccggac accccggaca ctacagattt aatagcactc agcgcagagc aggaaagggc
     2041 tatcgccgaa tttgatgcag atacccggcc ggccctgctg tttggcgcga ccggcagcgg
     2101 caagaccgag gtgtatctgc gtgccgccgc caaggtgctg gccggcgacg ccagcgccca
     2161 ggtgctggtg atggtgcccg aaatcaacct gacgccgcag ctgcaggcgc gtttcgagga
     2221 acgttttgcc cacctcggca aggcccaggt ggtggcgctg cacagcggcc tgacacccgc
     2281 acagcggctc aacagctggc tcgtcgccca cgccggccaa gcgcggctgg tgctgggcac
     2341 ccgcatggca atttttgcct cgctgccgaa tctgcgcctg atcgtcgtcg acgaggaaca
     2401 cgaccccagc tacaagcagc aggaaggcgc gcgttattcg gcgcgcgatc tggcggttta
     2461 ccgcgcgatg ctggaaagcg cacgatcaca ggcaggcgac agtgagagcg cggaggcttt
     2521 tcccttgcca gccggggccg cggaaccggc tttgccgggc cgcaggcgca gcgccccccc
     2581 gg
//
LOCUS       DAIDFG010000385         2572 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_385_length_2572_cov_19.289631, whole genome shotgun sequence.
ACCESSION   DAIDFG010000385 DAIDFG010000000
VERSION     DAIDFG010000385.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_385_length_2572_cov_19.289631"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          450 a          870 c          859 g          393 t
ORIGIN      
        1 agttcgcgtt ccgccaggct tttaccgatg ggcaggccgt tggccagcat gaaggtgttg
       61 gcaatgccgc cgcccacaat cagctggtcc accttcccgg ccagtgcctg gagaatggtc
      121 agcttggtcg ataccttgct gccggccacg atggcgacca gcgggcgctt cggattcgcc
      181 agggccttgg aaatcgcatc catttcggcc gccagcagcg ggccggcgca ggccgttttg
      241 gcaaactgcg cgatgccgta ggtcgaggct tcggcgcggt gcgccgtgcc gaaagcatcg
      301 tgcacaaaaa tatcgcagag cgccgccatc ttgcgcgcca gcgcctcgtc gttctttttt
      361 tcgccggggt tgacgcggca gttctccagc agcacgagtt caccaggcgc gacgtccacg
      421 ccgtcgaccc agccggactt cagcggcacc tcacgctgca tcagttcgcc caggcgctgc
      481 gccaccggcg cgagggagtc ttcgggtttg aactgccctt cggtgggccg gcccaggtgc
      541 gaggtcacca tcaccgcggc gccggcgtcg agggccatct ggatgcaggg aatcgaggcg
      601 cggatgcggg tgtcctcggt gatgtggccg ctatcgtcct gcggcacatt gaggtcggca
      661 cgaatgaaga cacgccggcc cttgacaagg ccttgggcgc acagatcgga gaaacggatg
      721 aatttcatgg gggctcgcaa ggaaacagaa ggtaaaaatc ggtgtgccag tgattgtagg
      781 agcgggtccg gtgaagccta ccagccgagg tagacatgca gcggcaggta caccgccatg
      841 ccggccacga tggtgccgag cacggtgcgg cgccagaaaa accaggcagc gcccgccagc
      901 gcggcaaaca cgcgcgcgtc ccgccaggtg ctgatcaaag atccctgcgt catcacgatc
      961 tcgggcacga tcaccgccgc cagcgcagca atcggcgcat agtgcagccc gcgctgggcc
     1021 caatccggca atgtccaggg tttgctcgac aggaaaaaga aagagcgcgt gatcacggtg
     1081 accaccgcca ggcccaggat ggtcaccaca gtccagccat cggtctgcat catgcggcct
     1141 ccccggcttt gggacggcct gcgtctggcc tggcgggctt ttcgagcatc aggcagaccg
     1201 ccacggccgc tgcgatgccg accaggatgt tgagcttgag cggcagggca aaggccgcca
     1261 ccgctgcggc gccggccaca ccggccgaga tcaggcgcag ccggctgctt gccagtgaca
     1321 gcgtgatgcc cagcaaggcc aggatgcccg caaaccccaa gccccagtgc agcggcacgg
     1381 cgttggccat ggcgatgccg agcaggctgg gcaccaccca ggcgacccag gtgacggcgc
     1441 agttgcccgc caggctggcc tcctgcgcgc gcagggtcgg cagatcatcg ggcggcaccg
     1501 gcttcggatg tcgggcggtg aacatcacat aactgaggtc ggtcgtgaag tacccgccgg
     1561 ccatgcggcg ccacagcggc tggtgcatca tgtacgggcg caggtgggcg ctgaacacga
     1621 caaagcgcag gttcacgcag gcagcggtcg ccagaatcac ccacatgggc gcgccggcca
     1681 caatcagcgg caccgccgcc agctgcgaac tgccggcaaa cacgactacc gtcatcagca
     1741 cgcactcgaa cacactgagc cccgacttga ccatggccac gccggtcatc aggccccagg
     1801 cggcggtgcc ggcagcaatg ggcgccatgt cgcgcacgcc ctggcggaac tcggggtggc
     1861 gccacagcgg ctctttgggg gtattcatgg tgcggcctcc gggtctgcac caaacaccat
     1921 gccaggcgcc agcgcgaagc cacgcaggaa ctgcgcggct tccagcgcct tgccgcccgc
     1981 cttctgcagg cgcgtcaggc gcagtacgcc gtctgccgcg gcgacatcca ccccctgctc
     2041 gctgacggcc agcacttcgc ccggccgggc gccggccggc acgggccccg gccacacggc
     2101 cgcctcccag atcttgagga tttcaccggc caccggactg gctgcggccg ggaagggatt
     2161 gaaggcgcgg atacgccgtg cgatcaccgc cgcactttgc gtccagtcga tggtggcctc
     2221 gtgtttttca atcttgtggg cgtagctgat tccctgctca ggctggcgca cgggggtcag
     2281 cccgccgcag gccgccagct ccagcgcctc gaccatcagc cggccaccca gggccgccag
     2341 ccggtcgtgc aggctgccgg tggtgtcggt cggcagaatc gcgattttct ccaccagcag
     2401 catgtttccg gtgtccagcc cgacgtccat ttgcatgatg gtgatgccgg tttcgctgtc
     2461 gccggcctcg atggcgcggt gaatgggcgc cgcaccgcgc cagcgcggca gcaaggatgc
     2521 atggatattg aagcagccca gccggggcgt gtcgagcacc cactgcggca gg
//
LOCUS       DAIDFG010000386         2548 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_386_length_2548_cov_16.662254, whole genome shotgun sequence.
ACCESSION   DAIDFG010000386 DAIDFG010000000
VERSION     DAIDFG010000386.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_386_length_2548_cov_16.662254"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          491 a          719 c          847 g          491 t
ORIGIN      
        1 cggcggcatc acggatcagg tccatggcca gccattcaac gaagaagctg gttttttcct
       61 tgacgttctg ctggtacagc gtgtgcagca tggcatgacc ggtgcggtcg gccgcggcgc
      121 aggcgcgctg caccggcttt tcgccgtagt tggcggtgtg gccgccgaaa gggcgctggt
      181 aaatcgtgcc gtcggggttg cggtcgaaag gcatgcccat gtgctcgagg tcgtacacga
      241 ccttgggcgc ttcgcggcac atgtactcga tcgcatcctg gtcgccgagc cagtcggagc
      301 ccttgacggt gtcgtagaag tggtagtgcc agttgtcttc ggacatgttg ccgagcgagg
      361 caccgatgcc gccctgggcc gccaccgtgt gcgagcgcgt cgggaacacc ttggagagaa
      421 cggccacgtt caggccggca cgcgccagct gcagcgaggc gcgcatgccg gagccgccgg
      481 ccccgacgat caccacgtca aatttgcgtt tgggaagctt ggaggatgca gtcatgattg
      541 ctttttcaaa agtcggtcgt caaaagtgga ggctcggttc cggggctcaa aggcgccaca
      601 gcacctggat gccccagccg gcgcaggcca ccagccacac gatcgtgaac acgtccaggg
      661 acagcttgag ccacaccggc ttgacgtagt ccatccagat gtcgcgcatg ccgacccata
      721 cgtggtaaag cagcgcaagg atcacgacaa aggtcagcac cttcatccac tgcgacgaaa
      781 aaataccggc ccagcggtca taaccgaggg gccccttggt gaagatcact tgagccagca
      841 gcaggatagt aaaaagggcc atcagggccg cggtgacgcg ctgggccagc cagtcacggg
      901 tgccgtaatg cgcgccgaca acgaggcgtt tggagccgta gttcacagac atgggttgct
      961 ccttgaatca gtagaggccg aacagcttgg cgcccagcac cagcgtcagc gcgatgctca
     1021 gtgccagcgt gaccagtgcg gtgcttttgc cgaattcgtt gctgagcctg tgggtggcgt
     1081 ccatgtagag atggcgcagg ccggcgatga aatggtgcag gtaggcccag atcagggcga
     1141 gggccaccag cttccagatg aagccgggca aacccagcat gccgatgttg aaggccgcct
     1201 tgaatttctc gaatgaaatt tcggaagaaa tcgaggtgtc gaacatccag atgatgaagg
     1261 gcatcaggat gaacatgatg gcgccgctgg cgcggtggag gatggacacc caggccgccg
     1321 gtgtcatgcg gtaggtggtc aggtctttaa aagcgttgat gttgcggaac tcgggccgct
     1381 ttgcacgctg tttggatgcc agctctgtca ttgttgggct ttcgtgggga gaggtctggt
     1441 aactgcctgg taactgcccg tgaagcacga cgcaattaca actgaaaacc ttcgaattct
     1501 attgcaacgc agcatccatc cggaccaaca ggacgatggc agcaggttat tgtcagaaaa
     1561 acggggcaag aacagagcca ttcgggcgcc gatggtcccg ggcctggggt aagctgactc
     1621 aagcattaca gctctccgct gcggccggca tgaactttcc tgttgctggt ttgttcgcgc
     1681 ggccgggcct tgcacggcag cgatgacttg ttgcccttga aacccatcgc tgctttcagt
     1741 tcagggtatt gcggtagtag tgggtgtcgg tgcggtaata accgcgccgc aactcaatcg
     1801 gcacatcgtt gtaggtgtag gcgatgcgct ccacgctcag caggggcgtg ccatgcgcta
     1861 ctttcaggag cagctcgcgc ccgtcgatgg ctgcttcggc gcgtattttc tcttcagcgc
     1921 gaaccatgcg cacattgaac tcggtctcga acagcgcgta catcgggccg tggtagtagg
     1981 ccagccgctc ggcggtcagg cccttgaacg gggcggcagg caaccagagg tcttcgagaa
     2041 tcgtcggtgt tccggcaaaa ctcagtacgc ggcgtgcctg cagcacggca tcgccggtgc
     2101 gcagcgccag cgcgcgggca atgtcggcgc tggcgcgcac gcgccggcag tcgatgatgt
     2161 cgcgctgggc cgggccttcg ctgcccggtg tgccgctgtc gggcacgagt ttgaggaaac
     2221 ggaactgcac atgctgttcg gcatgggtcg cgacaaacgt gcccttgccc tggcggcgca
     2281 ccaccaggtt gtcggccgcg agttcatcaa tggccttgcg caccgtgcct tggctcacgc
     2341 ggtagcgggc ggccaggtcc atctcggagg ggatggcttc gcccggcttc cactcgcccg
     2401 attgcaggct ttgcaggatc aggcccttga tctgctggta cagcgggctg aaagccggcg
     2461 tggcacctgg aaaatcgtca ggggaggggg tggggagggc ggccatggct tggggtgtga
     2521 atacctgcaa tgatatctta tataagac
//
LOCUS       DAIDFG010000387         2528 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_387_length_2528_cov_15.873837, whole genome shotgun sequence.
ACCESSION   DAIDFG010000387 DAIDFG010000000
VERSION     DAIDFG010000387.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_387_length_2528_cov_15.873837"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          465 a          785 c          804 g          474 t
ORIGIN      
        1 cacatcagcc gcgacttcct ggttccggcg acggcgtgaa gcaccaccgc gcgcctggtc
       61 acgccaggca agccacccct tcctggagcg cgcagtgaac gaacgtggga ctttccaatc
      121 aagcaggggc cgcggaaccg gctttgccgg gccgcaggcg cagaggcccc tttttcagtg
      181 cgaggggcag ggagctgccg caggctcgaa gcgagcgaac gtgggggccc tatgaagtta
      241 cacgtccggc acaccacgcg ttaccactac accgagcccc tgcgttatgc gctgcagacg
      301 ctgtggctga cgccgaagtc cggcccctcg caaaccgtcc ttttctggac gctgggcgcg
      361 ccgggtgtgc tgcatgagca gcatgacggc ttcgccaaca gcattcactc ctacaccttt
      421 gtcgccgaag cggcggctca cgtgaccggc agcctggtca acgcggcggg gcaggtcgac
      481 acgctgggca tcgccgaatt caccgacgac gcggcgatgc cgcacccggc ctttttcctg
      541 aggccgacgg cgctcgcggc gccgcatcct tcgctggccg agttcggccg ccgctttgtg
      601 ccgccgtcca cgcaggctgg tgttacggac ttgagcatgc ttttggccct gagccaaggc
      661 gtagcggacg ctgtcagcta tcaaaaaaat agcaccagcg tgaccaccac ggcgctgcag
      721 gcctttgaag ccggcgccgg tgtctgccag gaccaggccc atgtgatggt ggccgtctgc
      781 cgcagcctgg gcattccggc gcggtatgtc agcggttatt tttacgccgc caacgaaccc
      841 gacctcgcca gccacgcctg ggccgacatc tgcctcgacg tggcggcccg gcgctgggtc
      901 agcatcgacg tgacgcacag ctgccccatc gaccagcgcc acgtgcgtct ggccgtcggc
      961 accgactaca acgcctgccc gcccatcaag ggcatacgcc atggcggcgg ggaggaggag
     1021 atgttggtgg acatcacgat tgagccggtt tagggcatag ccgcgtgaca ctcgacaagt
     1081 tccgcatcat tgcgcagcag aagtgctggg gttcatatgc cacgtcactc ctacacgacg
     1141 caggcgcagt ctcagaggta cgtgacagct ttgggacgta ttggattgaa gcgggccatg
     1201 tcatgcgaga gcagattgca gatgatcagt tacttgtacg gctgctacgt cgcattcttt
     1261 gcccttacac gggaacgtcg atggagttgt accgcgggga aaaccggagc cgttggcaga
     1321 atcactccat tggtttggcg tggacgtcga gcatcgagac tgcgaggatg tttggtggcg
     1381 gactgaatgc ggttcaatcg ggtggcgtgt tgttgaaggc aagcttcaaa ccagaaacga
     1441 tcatcagcgg accaaacgac cacagcaagt atctggggga agatcaatac accatcgacc
     1501 cgtttttctt gtcggacctg tccgttgttg agttctttcc tccaaccaga taggttccaa
     1561 agcacagccg gtgcaacagc aagagtctgc gtggcagctc ttacttgaca atctcatttc
     1621 acccgcgccg ccacttcccg ccccagctca atcaccgcca tcgcgtaata actgctccag
     1681 ttgtagcgcg tgatggcata gaaattttca gtccccgcga cgtaactcgg cgcgctgccg
     1741 ccgttgttgg ggtcgccatt gagcagctcg atcagcgcca gcgggccggt gtggttcagc
     1801 gcctggcctt caagtactgc gcccctggcg gtgaagctgg ccatgctgaa ggtcggcaaa
     1861 atgtcggggg ccatcagcgt gtccatgtcc agcgtggccg catcaaaacg gaccgggtaa
     1921 tgcgtgggca tgccgggttg ccagttgaag gccttgaaat aactggcgac cgagccgatc
     1981 acgtcggcgg ggctgttcca caagtccacc ttgccgtcgc cgtcgaagtc caccgcgtac
     2041 ttgacccagc tcgaaggcat gaactgcggc atgcccatgg cgccggcata actgccgcgg
     2101 gggcccaagg ggtcgctgcc caggcggctc tggtggctca ggaactgttc gagttcgcct
     2161 ttgaaaaatg cgctacgttc gctggcgcgc ggatggctgg acggaaagtc gaaggccagc
     2221 gtggtcagcg cgtccagcac gcggaacttg ccggtgtcgc gcccgtaaat ggtttccacg
     2281 ccgatgatgc cgacgatgat gtcggcgggc acaccatatt cctgttcagc gcgcgccagg
     2341 ctggcctggt tgtcttgcca gaaagctgcg ccggctgcga tgcgcaccgg gtcgatgaaa
     2401 cggctgcggt agacgcgcca gttcttggtg aacgttttgg ccggcggctg catcagccgc
     2461 gcgacggtgg ggctgaagcg cgcctggccg atggcgttgc gcacccatga cacgtcgagg
     2521 tcgcgccg
//
LOCUS       DAIDFG010000388         2490 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_388_length_2490_cov_13.751951, whole genome shotgun sequence.
ACCESSION   DAIDFG010000388 DAIDFG010000000
VERSION     DAIDFG010000388.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_388_length_2490_cov_13.751951"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          395 a          808 c          837 g          450 t
ORIGIN      
        1 agcccgcgca gcagcagcgc cgattgcagc gcctcctcgg ggtcgccgag aaagcagacg
       61 gcgacgccat cgccggtgtc catcacgatg cgcgaggatt cggccacgcc cttgagcgcc
      121 ttgaggatca gttcgttgaa gcgctccctg agcgccattt gctgatccac cggatgtttc
      181 gaggaggcca ccagattcag aaacatcacg gtgcccagca cattcctgcg cagcgcggcg
      241 gctgccaggg ccggagtctg gctctccggg cccgcgctcg tggccgcagg cgtgtggcca
      301 gccgcgggta cgagaccggc ggggcggggt gcggcttcct ccagcgcagc ccgcaattgc
      361 gcgacgttct gcggccggcg gccctcgctc gggctcagcg cccagtcgat ggcgcgcagc
      421 agcgcctcgc cgaataccgc cgtattgccg atgtccaccg ccctcggcat ggtgtcgtcc
      481 tgcacgcgtg cggcggactc caccggtttt tgcccggtgg tcatccagta catcacggcg
      541 cccagcgaat agaggtcgct ccacggcccc tggttgccct tgctgtggta ctgctcgaag
      601 ggggcaaaac ccgggctcac gatattcgtc agatcgcgcc cgccggtggt gcggcgcgcg
      661 gcaccgaagt ccagcagcac cggcgtgccg tcggcgcgga tgtagatgtt gtcaggcttg
      721 atgtcgcggt gcaggaagtc cagcctgtgc accgcctcca gaccgtccag cagcggatgg
      781 atcaccctga gcagcccggc ctggtccagc atcggctggt cagccagcca gcgtttgagc
      841 gggtcgcccg actcgtactc catgacgatg taggccgtgc cgttttccct gaaataacgc
      901 agcacacggg tgatgttggg gtggcgaaag gacgccagcg cgcgcgcttc gtcgagaaaa
      961 cgctccagcc cccagtggaa ctgctgctcg gcctcgggca cacgcacacg cacggagagg
     1021 tcggccgcgc gggaggtcag gtcgcccgga aaatactcct tgagggccac cggcagctcg
     1081 aggttgatgt cgcgtgccag gtaggtgatg ccgaagccgc cgccgcccag cattttttcg
     1141 atgcggtact cgaagacggt gtgcccctcg ggcagggcgg ccgtgtccac gcccgccgcg
     1201 cagggcgggt tggcggaggc cggcgccagg tccatgggtt cagtttcccc gctctatcat
     1261 ctgcatggct ttttccaggc tgcggcgcat gcggttgaac gagctgccga gaaccgcgac
     1321 ttcgtcgcgc cccgcgtcgg agaactccgg cacgtcgaag tcgccggtgc tgaccttgtc
     1381 ggccgccagc gacatgcgcc ggatggggcg cacgatcagc cagctgagca tcaggttgag
     1441 cgcgacaaac accacggcaa acacggcggc cagggcggcc atgaaggtct tgaaggcgcg
     1501 gtcggccttg aggacgggaa tgtccatggg cacggtcacg acctgggcgc ccacgatctc
     1561 attgtgcttc cagccaaagc cgttggcctc gccgtagatt ttcagcatgc tggcgggcgc
     1621 cgcggccggc acgctgtggc actgcaggca ggccgcgttg ctgatctgga tgggtttggc
     1681 gatgtacagg atgcggccgg tgccgcccgt gcgctccgac accagctctt tcgactcgga
     1741 attctggcgg aactgctgga cgatgtcggc ttcccagtcg gtggcgcggt cgcgcgggtt
     1801 ggtcgggttc agggtggctt ccttgtagcc gtaatcgcgg tattttttct ggatgtggcc
     1861 cagggtttcg gtggcggcgt aggccggtac gctttgcggc aggaacacct cctgaagctg
     1921 cctgtccagg tgcggcttga cctgctcgac ggtgtaggcg cgcacggaca gcgccgtgtc
     1981 catcatcagg cgcgcattgc gcaacacctc ttcacgggcg ttgtcctgca gcagctggcg
     2041 cgagaccagg ccggtggccg caaagccggc caggaacacg atcaccagca ccagattgaa
     2101 cttaaggcgt aaacccatga cgtccccatt tcaaaaaaca tctcgttacc attttaggct
     2161 ttcccccttg ccgcccatcc ctccagtgac tcctgccgtc aggagacagg tggtgcagcg
     2221 ataatcgagg gatgtcttct tcccccaaag tcctgttcgg cttccatgcc gtcggcgtgc
     2281 gcatcaaaac cgccccgcaa tccgttttcg aggtgttttt cgacgtgtcg cggcgcgacg
     2341 cccgcatgcg ccagtttgtc gaccgtgccc gggaggccgg catccggctg atcgagtcgg
     2401 acgggctgcg gctggccaag atgtgcggca gccacggtca ccagggcgtg gtggcgcggg
     2461 tcgatgccgt gccgcaggtc aagtcgctgg
//
LOCUS       DAIDFG010000389         2477 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_389_length_2477_cov_16.917424, whole genome shotgun sequence.
ACCESSION   DAIDFG010000389 DAIDFG010000000
VERSION     DAIDFG010000389.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_389_length_2477_cov_16.917424"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          406 a          839 c          853 g          379 t
ORIGIN      
        1 ggcggtgccg gactcgagcg cacgcagcat gatgccgtgg cccggcagga tgatgccgcc
       61 caggaacctg ccggaggcgt cgatcgcctc gaccgtgacc gccgtgccca ccatcacgac
      121 cacgcagggt ttgcggatgc cgcggcccag aagccggtgc cgcgcgccta tcatcgcaac
      181 ccagcgatcg gcaccgaggc gggtcgggtg gtcgtagccg ttggtcacgc cggcctcctc
      241 ggcgctggaa acaacccagc gcggaaggac atcccagagc tccatctgct cctcgacacg
      301 gcgcttgacc gcgtcgccgg ccacgacgca gcccaggatg tgcgacggcg tgggcaggcc
      361 gcgccactcc tcgtccgcca gcttgtcgat gttttccaga aacaccgcgc catgggccag
      421 caggggcgac ccggccaccg gggccgcgta atgggcccac ttcagccggg tgttgcccac
      481 atcgagtgct aaaaaagtca tgccacccga ttatgaacag cttctggggc ttggggcgcg
      541 gacgcgccta gaggttttcc agtaaatcag ggtgcgacgg tcaccggtcg cctgaaatca
      601 acatcggcag gccgcgccgc ttcgtaggcg cgcgaggcgc gcagcaccag cgcgtcaccg
      661 aacatcggcc cgacaaactg caggccaatg ggcaggcccg cggcggtcag gccacagggc
      721 accgtgctgg ccggctgctg ggtcaggttg aaggggtagg aaaacggcgt ccagcccagc
      781 atggcggccg agtccatggg cgtatgcccg gcgggccgcg cctcaaacgc cggcacggcc
      841 acgctgggcg tgaccagcag gtcataacgc tgcatgaact ggcgcatgtg cgagcccagc
      901 gcgccgcgct gcagatgcag gcgctggatg tcgagcaggc tgaacttttc accgatcagc
      961 gcctgggcgc gaaagtcggg gtcggtcagc gcctgctgcg cgggggtgag cgtgttccac
     1021 accgtccagg caccggcaaa ccacaggccg gtggtgatct ccagcgggtc ttcaaagccc
     1081 ggatcgatct gctcgaccaa cgcgccgagg tcggccagca gcgccacggc gcgggccacg
     1141 gactgggcca cttcaggatc cacgttgcgc gcataaccga gcgccggcga ccaggcgatg
     1201 cgcaggccgc ggatgccgtc ttccagcccc tgcaggtagt tgctgctgtc gggcggcagc
     1261 gaggtccagt cccgggcatc cggctttttg agcacgttca tcatcagcgc cgcatcacgc
     1321 acgctgcggc tgtgcggccc caggtgcgcg accgaaccga acggcgacag cgggtaggcc
     1381 ggcacgcggc caaagctggg cttgaggccg aagttgccgc aaaacgccgc cggaatgcgc
     1441 accgagcccg cgccgtcggt gcctatggcc agcggcccca tgccggacgc caccgccgcc
     1501 gccgcgccac cggaagagcc gcccggggtc ttgctgacat cccaggggtt gcgcgtgatg
     1561 ccggtgcgcg gtgaattggt tacacccttg cagccgaatt ccggcgtgtt ggttttgccg
     1621 agaatcaccg ccccggcttc gcgcagccgc gcggtggccg gtgcatccac ctcccaggac
     1681 tggctggcgt ccaccgtgtg gctgccccgc agggtcgcca tgccgcgggt caggatcagg
     1741 tccttgatgg acaccggcac accgtcgagt tcggcgcagg gtgcaccacc ctggcggaat
     1801 tgctgccagc gcgcctcgct gtcgcgggcg ctttgcagcg cggactcctg cgccaccagg
     1861 cagaaggcgt tgagggcggg gttgagcgcg tcaatacgcg ccagcacggc gcgggtggcc
     1921 tcgaccggcg aggcctggcc gctttggtac agctgcagca gttcggtcgc cgtgcagtcg
     1981 gccagaccgg gcaaggtggg caaggttgag gaaggggtca tcagctactc cagtcgttgc
     2041 ttggtccgga cgacccgtcc ttcaaggacg gcgccggatc gaatcgggca ggtttttcca
     2101 gggcagatcg gccgggtccg ccgccatcgg ccccgcggct tttgcaatca ggatgtcggt
     2161 cgccgcattt tccaccagcg gcgtgaaatc agcgcggaag tggttggagc ttttgacgac
     2221 gatgattttc atgacctcgg gcgtgatgcc gaccatgcgg aacagctcgc ggtccagcat
     2281 ctgcatctgc ccgctggcca ccgccaccag gatgccgtca agctccagac aggcacaggg
     2341 accgaggttc gccgtgtgcc cggtcatcat caggcccttg agcgtgaccg tgccatcgct
     2401 cacagcgcgc accttgaact ggctctgcac cggggggtcg ctgtgttgcc cggtgaaggt
     2461 gggcaccgcc gtcccca
//
LOCUS       DAIDFG010000390         2432 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_390_length_2464_cov_18.045247, whole genome shotgun sequence.
ACCESSION   DAIDFG010000390 DAIDFG010000000
VERSION     DAIDFG010000390.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_390_length_2464_cov_18.045247"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          401 a          832 c          792 g          407 t
ORIGIN      
        1 ccccagggtg atcgaaatgg tcgcccccac cccggcggcg gcggcggcct gtgcagcctg
       61 cgggtcgaac atcagcccca gcgcaatctg gccgggaaat tttttcccgg cgccctgttg
      121 cagcagcgca tgcaacaggc cggtggtgtt gctgtcgccg ccggcgccgg ggttgtcctg
      181 cgtatcggca atgaccagcg gtttgcccgc ccgctccgcc cgcgccagcg attgcgccac
      241 ggcatcacgg gcagaaagaa attcaagtgt ccactgtgtc ggttcggcca ccagggcaaa
      301 caggcgatcc accgcttggg ccgcctgttc gccatgcgcc cagaccatgg gtccacactc
      361 agcaaagtcg gacgccggaa acgccatgca aaaactcagg caggtgccga aatcgcggtc
      421 cagctccgcc aaggctttgt agaggtctgc cgcgggctgc atccaggtgc tctgtgtgtt
      481 cagcggaatc aggaaaggca gacgccggta gtgcagcggc tccttgcggc ccagctggac
      541 gcggcgggcc agcagttgcg ccgccagctc accggtcgcg gccatgtcga tgtgggggta
      601 ggtccggtag gagaccagcg catcggcctg ctgcaacatg cgctgcgtca cattggcatg
      661 caggtccagg ctggcgacga tgggaatgtc ctggcccacc acccggcgga tgcgcgtgat
      721 cagctcgcct tcggtgtcgt ccacatgttc tgccacgccg gcgccgtgca ggtccaggta
      781 aaccgcgtcg aagccgcccc ggcccacgtc ggacagcacg gccgcgctga tgcgctcgaa
      841 cgcatcctgc gtcacatagg aggagggcga tgcgccgcac cagctgctgg gcaccagttg
      901 ccagccccgg ctttttgcgg catcaatgaa gccccccgcc ggcaggttga cgccggtgag
      961 cctgtccagc atcgcctggc catggataaa ggcagggaaa tcatcgcccc ggttaaacgc
     1021 cgcccagtcg gccttggtgg gcgcaaaggt gttggtttca tgctggtaac cagcaacgag
     1081 gactttcaga cttttcaaaa tatcgctccg ggcgccgccg cgaacagccg gcgtgtgtag
     1141 tcgtgttgcg gttgcgcata cagctcggtg gtcaggccct cttccacaat ttcgccctgg
     1201 tgcatgacga tcacacggtc gcacagctgc gccgccacgc gcaggtcgtg tgtgatgaac
     1261 aacatgccca ggcccagctc ggtctggatc tcgcgcagca gcttcaaaat ttgtgcctgc
     1321 acactcacgt cgagggcact gaccgcctcg tcggccacca gcacacgcgg ccggcaggcc
     1381 agcgcgcgcg caatcgcgat gcgctggcgc tgcccgcccg agaactggct ggggtaacgc
     1441 tgcaggcctt cggcgggcaa ccggaccttg tccatcaaac tggccgcgag cgccatcgct
     1501 tctgcgcggc tagctccgaa attcatggca ccttcgatga tggaatcacc gaccttgcgg
     1561 cgcgggttga gtgaacggtt ggggtcctgg aacaccacct gcacctggct gcgcaagggc
     1621 cggagccggc cttcactgaa ccgggccacg tcgagcaggg catcgtcccc gccccctgcg
     1681 ttctggcgcc acaggacctg gccaccggta gggtcgatca gccggatcag gcagcgcgcc
     1741 agcgtggact tgcccgaccc ggactcgccg acaatgccga cggtttcccc ggccgacacg
     1801 gccaccgacg cggccttcag cgcatgcgtc agccggctac ggcccagcca gtcgcgggcg
     1861 ctgtaggttt tgctgacgtc ctggccgcgc agcagcggcg cgccctgcgg cgccggccgg
     1921 gccggcggcg gcgtcatgct gggcacggcc gccagcagca tcttggtgta gtcggcctgc
     1981 ggcgccagca gcactgcatc ccgcgtgccg tactcgaccg cccggccctg gtgcatcacc
     2041 agcacctcgt cggcaatctc ggccaccacg cccatgtcgt gcgtgatgaa cagcacggcg
     2101 cagcgatggc cacccgacag gtcggcctgc agctcctcaa tcagccggag gatttccttt
     2161 tgcgtggtga cgtcgagtgc cgtggtcggt tcatcgcaga tcagcagggc cggctgcaaa
     2221 atgacggcca tggcaatcac gatgcgctgg cgctgcccgc cagagagctg gtgcgggtag
     2281 ctgcgcatca tgcgttcagg ctcgggcagt ttgacacgcc gcaagatgcc cagcaccgct
     2341 tcgctgcgcc gcttcgcgcc ccagtcggtg tgctgcctga gcagctcgtc gatctggtcg
     2401 ccgcaggtca tgaccgggtt cagcgcggtc at
//
LOCUS       DAIDFG010000391         2458 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_391_length_2458_cov_13.029546, whole genome shotgun sequence.
ACCESSION   DAIDFG010000391 DAIDFG010000000
VERSION     DAIDFG010000391.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_391_length_2458_cov_13.029546"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          401 a          795 c          824 g          438 t
ORIGIN      
        1 taggcgatct ggcccatgac gagacgatcc gccccggcac cacgctggaa ggccttgccg
       61 ccttgcgcac cgtgcaggaa ggcggggtca ttaccgcagg cgtcgccagc cagaactgcg
      121 acagtgccgc ggcgctgctc gtggcgagcg aacgcgcggt gaaggaacat gggctgaaac
      181 cgcgtgcgcg cattcaccac atttcggtgc gcgccgatga tccggtttgg atgctgaccg
      241 cgccgatccc ggccacgcaa tatgcgctgg ccaaggccgg gatgaccgtt gacgacatcg
      301 acttgttcga atgcaacgag gcgtttgcat cggtgccgat ggcgtggatg caggagctgg
      361 gcattccgca tgacaaggtg aacatccacg gcggcgcgat tgcgctgggc catccgctgg
      421 gcgccaccgg cgcgattcgt gccgccacgg tgatccatgc gctgcaccgg caccagctga
      481 agtacgggat ggtgaccatg tgtgtgggga tggggcaagg cgccgccggc atcttcgaac
      541 gggtgtagta gcggctactg cgcgggcgca ttgcgtcgtt gcagccccaa gaaaatcctc
      601 acgacccttt aggtcgctcc ggttttcttc gggcacccgc gcctcgcact gcatcccgct
      661 cgctacgccg taaaggcatt gtggtttgtt ggaggaaaaa tcatgcaaca gcaggttctg
      721 gaatcgggca cttcccaact cgccctgcgg gtttacgagg ccggcacgct tgcgcgggcc
      781 agcgtcgtca ttggcggcgc gatgggcgtg cggcaggact attacgcgcc gtttgccgag
      841 tggttgtcgg gccagggctt ccgggtcacg acctttgact accgcggttc cggggagtcg
      901 ctgccgggta cgccggatgg ctcgctgcgc gggttccagg ccgatttgtt cgactgggcc
      961 agcgactacg acgcggtggt ggcctcggcc aaggccgcct tgccggatgc gccgctttac
     1021 ctgctgggcc acagcctggg cgcgcaactg ccgggttttc tgaaaaacca gcaccaggtc
     1081 gacggcctgg tcagcattgc cgccggcagc ggttactggc gcgacaacgc accgcaactc
     1141 aagcgcatgg tgctgtactt ctggtacgtg ctggtgccgc tggccacacg cctgtgcggc
     1201 tatttcccgg gacgcaaact gaagaaagtc ggcgacctgc cggccggcgt gatgctgcag
     1261 tggcgaaaat ggtgcctgaa cccgctttac agcgtgggcg ccgaaggcga aggcgcgcgt
     1321 ctggcctatg cgcaggccag gtttccggtg caggcgctgt ccatcaccga cgatgaactg
     1381 atgacctggc gcggcacgca gaacctgatc aacctgtaca gcggcgcctc gcgcagtttt
     1441 gagcgcatca cgccgggcga gctgggcgtc aagcgcatcg gccatttcgg ttttttcagg
     1501 caacagttcc gtcagaacct gtggccgcac acggtgcaga ttctggagca catgcgccag
     1561 gcccgtccgg tggctggcgt ccccggcacg actgcctgag accgacggcg gcggcatact
     1621 ccgaccatga gttttcatcc ttttgatacc gcggtggccc tgcaggcgca ggccgacggg
     1681 acatttctcg gtcacaccag cgccgcctat gccaacatgg tcgggccctt cggcggcgtc
     1741 acggcggcgc aggccttgaa tgccgtgctg cagcatccgc agcgcctggg ggatccgatt
     1801 tcgctgactg tcaacttcgc cgccgcgctg gcggacgggc cgtttaccgt gagcgctcga
     1861 cccgcgcgca ccaaccgctc gacccagcac tgggtgatcg agatgcaaca ggacggcgag
     1921 acggtgctga ccggcaccgc ctttttcggc ctgcgacgcg acacctgggg caccgacgaa
     1981 cacgccatgc cggccgtcgc ggcccccgcc gatgtcgccc gggccgatgg cccgacccgg
     2041 gttgagtggg tccaacgtta tgagatgcgt tttgccgagg gcgcgattcc caccgaatgg
     2101 cacggggccg accagggcgc cagccgcacc ctgctgtggc tgcgcgacga cccgccccgg
     2161 ccgctggatt tcgcctcgct cacggcgctg tcggatgtgt tttatccgcg cgtctggcgc
     2221 cgccgaccgc tgctggtgcc ggtaggcacg gtgtcgatga cggtgtatta ccatgccgat
     2281 acagccctgc tgcaggccac cggtagcggc tacctgctgg ggcagacgca ggcccaggcc
     2341 tttcgcagcg gctatttcga ccagacggcg cagttgtgga acgaggccgg tcaattgctg
     2401 gtgaccacac accaggtcgt ctactacaag gaatgagggt ggcgcccgcc atcatccc
//
LOCUS       DAIDFG010000392         2457 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_392_length_2457_cov_22.767694, whole genome shotgun sequence.
ACCESSION   DAIDFG010000392 DAIDFG010000000
VERSION     DAIDFG010000392.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_392_length_2457_cov_22.767694"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          431 a          786 c          803 g          437 t
ORIGIN      
        1 atcgccggcg tgatggaact cggcatggcc gggcgcggcg agcatgtcga agtgatgccg
       61 ttcctggaaa accaggtggc gtccgaactg tccggcaacg tcatcgacct gtgcccggtc
      121 ggcgcgctga ccagcaagcc cttccgctac tccgctcgca cctgggagct gtcgcgccgc
      181 ccctcgatca gcccgcacga cagcctcggt tccaaccttg aagtccacgt caaggacaac
      241 aaggtcatgc gtgtgctgcc gcgcgagaac gaggacgtca acgaatgctg gctggccgac
      301 cgcgaccgct tttcctatga gggcctcaac acggccgagc gcctgaccca gccgatggtc
      361 aagcacgagg gccagtgggt ggaaaccacc tggcaggcgg cgctcgagct ggtcgctaac
      421 aaattgaaag ccattccggc cgagcagatt ggtgcactgt cgacgcccta ccgtccggcg
      481 gaagagctct tcctgctgca gaagctgatg cgtggcatgg gcagcggcaa cgtcgaccac
      541 cgtctgcggc agtcggactt ttcactcgac gacaaggcgc atggcggctt ctggctgggg
      601 atgcccgtga cctacatgtc gcgcctgaac cgcatgctgg tggtgggttc caatctgcgc
      661 aaggaccatc cgttgatggc ccatcgcatc cgcgagtcgg ttcgctggtc cggcgaactc
      721 aacctcatca acgcagcgga agacgaattc ctcggcaagg tgcatgccaa gcgcatcgtc
      781 gcgccgtcac agcttgccgc tgcgctggcc ggcgtgtgcc gtgcgctggc cgaactgaag
      841 aatctgccgc tgcccgtcct tgccgcacac ggtgtcgtcg atgacaccgc gaggaagatg
      901 gccgcgagcc tcgccggcgg tgagacccgc gcggtcttcc tcggcaacat ggcgcagcat
      961 cattccagct acggacagat acacgcgctg gcgcaggaag tatcgaagct tgccggcgcg
     1021 agttttggtg tgctgggcga ggccgccaac agcgtcgggg cggtggcggt tggcgcgatc
     1081 ccgggctgtg gcccgctcgg gcagccagcc gtcaaggggc tgaacgcgca gcagatgctg
     1141 gccaatccca tgcgtgccta cctgctgctg ggagtcgaag ccgaactcga tacccacgac
     1201 ccggtgtcgt cgatggccag catcaatgcc gccgaattcg tcgtggtgat gtcgccctat
     1261 aaaggcaagt cgctcgacta cgccgacgtg ctgttgccga ttgcgccgtg gaccgaaacc
     1321 tcgggcacct tcatcaatac cgaaggccgc gtgcagagct tcagcgccgt ggtcaagccg
     1381 atcggcgaaa cccgcccggc gtggaaggtg ctgcgcgtgc tgggcaacct gctgggactg
     1441 gcaggtttcg atcacaacga ctcgaaggac gtgctgcgcg acgcgatcgg cgataccccg
     1501 acaggcaacg tgcaggcctt cctaaataat gaagtcagcg gcgtgacggt ggcgccgccc
     1561 cagacgcatg aaggcctgga gcgtgtcgcc gaggtgccga tttaccagac cgacgccgtg
     1621 gtgcgccgct ccccctcgct gcagatgacc caggatgccg cattgcctgt ggcgcgcatg
     1681 cacagccgcc tgatcgcgaa gctgggcctc acggaaaacg gccgcgtctc ggtgcgccag
     1741 acggcttgcg cgctgaccct gaaggtgcaa cgtgacgacc tgctgcccga tacctgcgtg
     1801 cgcatcccca gcggtcatcc gctgaccgcg ggtctgggcc cgatgttcgg cccgatcacg
     1861 gcggagtcgg tctgatggag agcgtgggta tggactggag cgccattcaa accgtcgcct
     1921 ggacgctgat caagatcatg gcgctggtga tgccgttgat gctgggcgtc gcctacctga
     1981 cctatgcgga acgcaaagtc atcggctgga tgcaggtgcg catcggcccc aatcgcgtgg
     2041 gctacctggg tctgctgcag ccaattgccg acggcttgaa gctgctgatg aaggaaatca
     2101 tcattcctgc cggcgccaac aagagcctgt tcattctcgg tcccatcctc gccatcgcgc
     2161 cggcgctggc ggcatgggcg gtgattccgt tctccgacac gctggtgctg gccaacatca
     2221 atgccggcct gctgtatgtg atggcgatta cctcgatggg cgtctacggc gtgatcatcg
     2281 cgggctgggc gtccaactcg aagtacgctt tccttggcgc gatgcgctcg gcggcgcaga
     2341 tcgtgtcgta tgaaatcgcc atgggcttcg cgctggtcgg cgtgctgatg gccgcgcagt
     2401 cgctgaacct caccgacatc gtgcaggggc agtcgggcgg cctccaccag tggtatc
//
LOCUS       DAIDFG010000393         2415 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_393_length_2415_cov_14.584746, whole genome shotgun sequence.
ACCESSION   DAIDFG010000393 DAIDFG010000000
VERSION     DAIDFG010000393.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_393_length_2415_cov_14.584746"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          528 a          807 c          730 g          350 t
ORIGIN      
        1 ttccccgact acaacgaccc gctgctggtg gaaagcggcc tggtcatcac cggtgagcct
       61 ggcgaggaaa atgcccaagg cgaagccaag cgttacgacc gcttccgcga ccgcatcatg
      121 tttccgatcc gcaacgtcaa gggcgaatgc ataggtttcg gcgggcgtgt gctgggcgac
      181 gaaaaaccca agtacctgaa ctcgcccgaa accccggtgt tcagcaaggg ccgcgagctc
      241 tacggcctgt tcgaggcgcg caccgcgctg cgcgaacacg gctacgcgct ggtcaccgaa
      301 ggctacatgg acgtggtggc gctggcccag ctcggttttg ccaacgccgt ggccaccctg
      361 ggcacggcct gcaccgccga ccatgtgcaa aagctgttcc gcttcaccga gtcggtggtc
      421 ttcagctttg acggcgacag cgccggccgc cgggccgcgc gcaaggcgct ggacgccgcc
      481 ctgccctttg ccaccgacgt gcgcaccgtc aagttcctgt tcctgccggc cgaacacgac
      541 cccgacagct acatccgcga acacggccag gaaggctttg cggcctatgt cgccaaggcc
      601 gtgccgctga gcaaattcat gctcgacgcc gccggcgaag gctgcgacct ggataccgcc
      661 gaaggccgcg cccacatggc cagcaatgcg cgtccgctgt ggagcctgct gcccgacggc
      721 acactcaagc accagttgct cgccgagatc gccgacaccg tgctgatcga cagccgcgaa
      781 ctgctgcaga tctggcaacc ggccgcgccg cactataaaa acagtagcaa ctcgcgaaca
      841 cagggaaagg gccagcggcc aaaagcgcct gaatatgccg acgccgttcc gcatttcgag
      901 gagttttccg gcaactttgc cgacttgcac gatgaaggcg cttatgccgc gtcgctgccc
      961 gccccgtcgt cggcgggcgg gtctgccggc cgcagcttcg gcgccaagcc aggtgccagc
     1021 cgcccgccgc ggcgcatcgc ggggcgggtg ttgccggccg gccgcgaaga ccgggtgctg
     1081 cggctgatgc tgaccgaccc gcaaagctgg gacaagctca gcggggaaga acacgtgctg
     1141 ctgtcggaac tgccggcgcc gcacgggccg ctgtttgtct ggctggagag ccagctgcac
     1201 gaacacgggc cgcagccctg ggcagccctg cgcgaagggc tgcgcgaaca cccccaggaa
     1261 aaatacgcgg tggaccagat cgcccagctt cacgagggca tcgagtgcga ctggaacgag
     1321 gtccgcgcca tcctggacca gctcctcaag ctcaaacgcc agcaggaaat ggtcgatttg
     1381 ctgccccggg tgctggccga cccggcggcc aggcagcgtt acgaagagtt gtccgccctg
     1441 ctgaaaaata gctgatttgg cccttttttg ttgcccaaaa atgaggcaac agggtataat
     1501 ccgattttcc gctacggcaa gcgcgacagc agcacctccg gtaccagccc cccgcaagaa
     1561 cgggacctca caggttgaag ggccacctcc ccgcaaggac tgcacaccaa atgttcgccc
     1621 aaacaacttg ctgtggagcc tgacgggctt gagacacctc tcaagtccga cagactcctg
     1681 aatgcccagc catccggcgc gggcattttt tgtgcctgta gcccgaccgc tgtcgggtgt
     1741 caggaaccca agtctgttga ggaaagtttc acaccggggg agttcgccat gcctgctcaa
     1801 aagtccagca agactgccaa aaccgtgacc aagcccaaag cggcagccaa ggctgccgcc
     1861 cgaccgccgg tcaggaaagc cgccagcaac aagcttgaaa aacccggaaa aaccgccata
     1921 tccagaccag ttcctggcaa aaaagatgca gccagcaaag cgcccctgaa atctgaaaag
     1981 cctgtcaaga aagtgccccc tgtgccgacc aagtctcctg cctccaccgc caaacctgct
     2041 gccaaaaaag aagccgagct gaaggtcacc ccgaccgcag ccggcaccga cgtgcctgtg
     2101 aaaaagaaac ccggtcgccc gccgaaaaac cccgctgccg cggtggaagc cggcggcgcc
     2161 aagcgcggcc gcaagcccaa ggtggccatc aagggtgacg gcgacgagga catgtccgac
     2221 atcgaggacg atctggtcgg cgaaccggtg gtggaaacga ccgaaaaggt caaaccgctg
     2281 cgcatgaaaa tcagcaaggc gaaggaacgc gccttgatga aagagttcgg cttggacgag
     2341 accgtcctgt ccgaagaaga catggccaag cgccgtgcac gcctgaaggc actgatcaag
     2401 ctcggcaaga cccgc
//
LOCUS       DAIDFG010000394         2404 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_394_length_2404_cov_16.189017, whole genome shotgun sequence.
ACCESSION   DAIDFG010000394 DAIDFG010000000
VERSION     DAIDFG010000394.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_394_length_2404_cov_16.189017"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          417 a          767 c          825 g          395 t
ORIGIN      
        1 tgaccacgtc ggcgccggtc tccttgtcgt gctggctgat gcgccacagg gtggccaggc
       61 gttcactgat gaggtcctgg tgcggcgaga aggtgtcgta gggcagggtt tcccagtcgg
      121 gaaacagggc gcagcgcagg gccggtgcaa aaaacgccag ttcgtcgagc aggcgttgcg
      181 cggtggcggc gtccgccgtg acgatggccg tgagccggcc ggccgctttt tccctcaggc
      241 ccagctgcgc cagcaacagg gcatcggcgg aaccagcggg ttgcggcagg gtgtagcgtt
      301 tgccagggag cagtttgggt agatccatga aacagtcgga ataaggtaac aggaaagcgg
      361 cggaaaaaag gaaaacaccc caaacggtgc agagtggggt gtgaacggcc ctggctggcc
      421 tttgacgccc cgattttaga atctatgatc aagccatgac gccgatcccg ccgaaatccc
      481 tggttccccg cttttttgcg ctgattccat gcgcgggtca gggcatgcgc gccatgggtg
      541 acaacgcggc acagggcgca gccgcggcct tgcccaagca gtaccagccg atagccggcc
      601 tgcccatggt gcggcacaca ctgcaggctt ttgccgcggt ggcgcgcatt gattcctgcc
      661 tcgtggtggt ggcgcctggc gacgattttt tcagcgggca gaccgccacc ggctatgagg
      721 tggcgccctg cggcggcgcc acacgcgccg ccagtgtgct caatgggctg gagcaactgg
      781 ccgccagcgg cgccgaactc gatgactggg tgctggtcca cgatgccgcg cgttgcctgg
      841 ttacgcccgc gctgatcgac cgcctgatcg acgcctgcca ctgcgacccg gtgggcggcc
      901 tgctggccca gccggtaccc gacacgctca agcagggccg ggacgaacgc gtggtcgcca
      961 ccgtggaccg ctcggacaaa tggctggccc aaacgccgca gatgttccgt ctgggcgccc
     1021 tgacgcaggc cctgaagatg gcgcaaaccc tgggcgagcc ggtgactgac gaagccagtg
     1081 ccatggaagc ggccggcgtg gcgccgcagc tggtgcgcgg cagtgcccac aacttcaagg
     1141 tcacctggcc ggaagatttc ttgctggccg aagccctgat caaaagccgg acgccggcca
     1201 agccgcgggt gcgcaaaccc gccgcaccga aagctgcaca gaaagtttta tctgatgaca
     1261 ccagcaccga acccagctga ctccaaacgt ggggagagcg ttggggctcc attccgcatc
     1321 ggtgaaggct gggatacgca cgcgctggtg ccgggccgca agctgatcat tggcggcgtg
     1381 accatgccgc acagcaccgg tctgctgggc cactcggatg cggacgtgct gatccacgcg
     1441 atcatcgacg cgctgctggg cgccgccggg ctcggcgaca tcggcagcca tttccccgac
     1501 accgatgttc gcttcaaggg cgcggattcg gtgctgctgc tgcgcgagac cggtcgcctg
     1561 ctggctgaac gcggacttcg catcggcaac atcgacagta ccgtgatcgc gcaggcgccg
     1621 aagctggcca gccacattcc ggccatgcgc gagcgcatcg cacaaaccct gggcctggag
     1681 gccggccggg tcaacgtgaa agccaagacg gccgagaagc tggggccggt ggggcagggg
     1741 ttgagcatcg aagcgcgtgc tgtggtgcta ttgttttagt agctgcttgc gaccgcccga
     1801 aaagggctgg aaccctgttt cattcacagg tggccgactc cctctcccct tgggagaggg
     1861 caggggtgag ggctacagcg tgtgccaccc ctggccgagc gggtcttgcg aggcgcgggc
     1921 cctcacccca accctctccc gggggggaga gggggcagac aggcgtgggg gtcaacccct
     1981 ccttaacttg atgtgcgcag ccagtccgcc cgaactggtg ttggccagcg cgaacacgcc
     2041 gcccatgcgg tgcagggttc gctcgacaat cgccaggccc agcccggccc cggtggccgc
     2101 cgtgcgggcc gcgtcgccgc ggaaaaaggg tttggtcagg ttgttcaggg cctgcggcgc
     2161 gacgccggtg ccgtggtcgc gcaccttgat gagcacccac tggtcgcgtt ccttggctgc
     2221 aatgtcgacc atggcgatgc cggtctccgg cgtcttgcca taacgccgcg cgttttccag
     2281 caggttggaa atcacgcggg acagttcgac ctcgtcggcc agcaccacaa tctggcccgg
     2341 cagcgacaca ttgacacgca tgtcggcgta ttcgcccacc gaatagacgg ccgcctcgat
     2401 gatg
//
LOCUS       DAIDFG010000395         2402 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_395_length_2402_cov_18.249680, whole genome shotgun sequence.
ACCESSION   DAIDFG010000395 DAIDFG010000000
VERSION     DAIDFG010000395.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_395_length_2402_cov_18.249680"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          360 a          777 c          807 g          458 t
ORIGIN      
        1 cggcctggtg gcggtggcgg ccggcgccgg cggccatgcg ggcgtcaaaa gcccgtttgc
       61 actgatccag gaaatccgcc agtggttcga cggcccggtc gcgctgtcgg gtgccatctc
      121 caccggcggc gccgtgctgg ctgcccaggc catgggtgcc gacttcgcct acatcggctc
      181 ggccttcatt gcgactgaag aagcgcgtgc ctcggacgct tacaagcagg ccatcgtcga
      241 cagcaattcc gacgacatcg tctacagcaa cctgttcacg ggcgtgcacg gcaactacct
      301 ggccccgtcg atacgcgcag ccggcatgga ccccgacaac ctgcccgaga gcgacccgag
      361 caagatgaac ttcggcggcg acaagtccaa ggcctggaaa gacatctggg gctgcggcca
      421 gggcattggc gcgatcagca aggtgcagag cacggccgac tttgtcgcgc aactcaagcg
      481 cgagtaccag gaagcgcgcc agagggtctg cgcttaagct ttctgccgca tgaagccgcc
      541 ggttgctgcc ctgacaccgt ccacgcagcg cctgcccggc attgatgccc tgaaggcgct
      601 gggctgcgtg ctgatcgtct ggcaccacct ggcgttttac ggccccatga gcgacgtggt
      661 ccacaccgcg gcgccggggc tgatgaactg gctttacgac tacgcccgca tggcggtgca
      721 ggtgtttctg gtggtgggcg gttttctggc cgccagcgtg ctggcgcccc agggcgttgc
      781 ggtgttccgg caacccggcc gcctgatcct gcgtcgctac cagcggctgg cgccgcctct
      841 cttcgtggcc ctgttggtca ccacgctggt tgcggcgctg gtgcggccat ggctgccgca
      901 tccttcggtg cccgacattc ccagcctctg gcaggtgctg gcccatgtgt ttttgctcca
      961 ggacatcgtg gggcaggagg ccctgtcggc cggcatctgg tacgtggcga ttgattttca
     1021 gctgtttgtg atgacggtgc tgctgttcac gctggccagc cacacccaca ggcgctggcc
     1081 cggcctgccg gcgcaggccg gggtcatggg ggtggcgctg ctcgcagccg cgtcgctggc
     1141 ggtgttcaac cggcacacgg cgctggacat gacggggctg tatttctttg gcgcttacgc
     1201 cctgggcatg ctggcctggt gggcatcgcg cagcccccgg ccggcgcgct ggctgctggt
     1261 cattgtggtg ctgggcgtgc tggcgctggt gctcgacttc cggggccggc tgctggtggc
     1321 gctggtggtg gctttggggc tggtggtgct gcagcgccgc cgaccgctgg cgcgctggct
     1381 ggaggcgccg tggctgctgc aactcgggca gatgtcctac tcggtgttcc tgattcactt
     1441 tgccgtgctg ttgggggtca atgccgtggt cagtcagctc tggcccacgc agctcgtggc
     1501 caatgcgctg ggcatgctgg cggccttcgg cctgtcgctg ctggcaggca aggcgctgta
     1561 cctgggcgtg gagtcgcggc gccggccggc gcgtgtgccg ccccgggtgg gcgtcagcgt
     1621 ctcctgaatc ccctgatttt taatgaaaag tgcttccagc ccttgaagga tgggcgcaag
     1681 ccgctattga ataagtagcg cttcagcctg caattggtga cgggcggcag gacggcggcg
     1741 gagtgactat actgtttgtt tgtacagtat tttctgtgcg tactcatgac cgaagtcacc
     1801 attcccctgc acgccatcaa gggccgcggt acggccacgc gtctggccca ccggttcgag
     1861 aaagacacgc gggccgctta tgacgacggc tggggcacgc tcgaagagtc agccgcgaac
     1921 caggcgccga tagcgaccga ggtggtgttc gaggacgcac gcagtgccat ctgccgcaat
     1981 gattcgcccg acatctactt cgactattcg gtcaacccct accgcggctg cgaacacggc
     2041 tgcgtctact gttacgcgcg gccgacgcac agctacctca acctctcgcc gggcctggac
     2101 tttgaaacaa aaatcatcgc caagcgcaac atcgccgcca tgctgcgcgc cgacctcggc
     2161 aaacgcagct acgtgcccaa gctgatcaac atcggttcgg ccaccgattg ttaccagccg
     2221 gtcgaacgtg agctgcgcct gacgcgcggc ctgatcgaac tgatgcagga agtgcggcat
     2281 ccgctggcac tggtcaccaa gtccagcggc gtcgagcgcg acctggacct gctggcgccc
     2341 atggcggccg accgtctggc cgcggtctat gtgaccatca ccacgctgga cggcgaactc
     2401 gc
//
LOCUS       DAIDFG010000396         2402 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_396_length_2402_cov_16.134640, whole genome shotgun sequence.
ACCESSION   DAIDFG010000396 DAIDFG010000000
VERSION     DAIDFG010000396.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_396_length_2402_cov_16.134640"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          465 a          780 c          725 g          432 t
ORIGIN      
        1 tctgcggcca gtttttttcg tcctgggccc ggatgcgttc catcagcaca ctgcgctcgc
       61 tgtcgctgca gccggccagg taggcccgcc ccatcgccgt gctggccagc ggaatgcgtg
      121 atccaacgtc caggctcagc gtgagggccg attcgctgcg gcagttctcg acataaatca
      181 tgctcaggcg gtcgcgtatg cccagcgaaa ccatggcctg cgaactgtcg gccagctcct
      241 gcatcatcgg ccgcgccagc tggcgcatgt ccatgcgggc cagcatggca ctgcccagcg
      301 ccagcaccgc actgccgagc cggtagcccg gattgccgct gccgtcctgg gcatgttcga
      361 ggtaaccctg tttggtcaac gtgtaggtca gtcgtgaaat ggttgacttg ggcaggccgc
      421 aacggcgcgc cagttcctgg ttgcccaggg tgcggtcgcg cgaacggaag gcactgagca
      481 cgtcgagtcc gcgcgccagc gcggtcacaa aatgccggtc ttccttcgca ttcccggcgc
      541 cgggtctggc cgaagcgacg tgttctgacg tgagtacttt tttcatattg ttctgcaatg
      601 cggaatatgg gaattttatc aggccatgtc gcttgagaga ctaaatcccc aatgtaatac
      661 gggaattcca tagcgtcgtc cggagctcgc agtcacacaa ttcatcagca ttgcaacttc
      721 aatattctgc agtgcagaac catgaaaaca caacagcaac gaggagaact tcatatgtcg
      781 agcaaaaaaa ttcatgtcct ggcgctggcg ctgagcctgg ccgccaccgt gttttcggcc
      841 catgccgcac ccggccggga ggatctggtg atcgggcagg tggcgccgct gaccggcacc
      901 atcgccggaa ccggcaacga atacgtggcc ggcggagcgg cctatttcgc ctgggtcaac
      961 gacaacggcg gcatctacgg ccgcaagatc cgcgtcacgc tcaaggacga cagctacaag
     1021 ccggaccaga ccctggccct gacgcggcaa ctgctggccg aggacaagcc gctggccctg
     1081 ttcggttttg tcggcaccgg caacgtgctg gcgctgaaca aaaacagggt gctcgaagat
     1141 gccggcatcg cgctgctggc gccctacacc ggcgcgcaga acctgcgcga gccgatgaac
     1201 ccgaacatct tccacatccg cgccagctac accgacgaaa ccgcgcgtat ggtggagcac
     1261 ctgcacacga tcggcctgcg ccgctttgcc gtgatgtacc aggacgaccc gttcggcaaa
     1321 agcggactgg ccggtgcgga aacagcgctg cagaaactgg gcatgtcggc ggtggccaag
     1381 ggcggttatg accgcaccaa acccgaagac gtggatgccg ccgtggccgc catcgcgccg
     1441 gccaaccccg acgccatcat catggtctcg gtcaaccggg cttccgccgc cttcatcaag
     1501 aaaatgcgcg cccagggcaa caaggcgcgc ctcttcagca tctcggtggt gaacttcaag
     1561 gaactgctga aaaacgccgg cgaggacaac gcgcgcggta tcggcatatc ccaggtcatg
     1621 ccctacccct actccacgct ggcaccggtg gcacgcgagt tccagaccct gatggccaag
     1681 taccagcccg gcaaggtggt gtcttacgcc agcatggagt ccttcatcgc ggccaagatc
     1741 ctggtcgaag ccatccgccg cagtggcgcc gacccgacac gcgcaaaaat catggcccag
     1801 ctcgaaaaaa tgaacagcta cgacgccggt ggtttcaagg tgagcttctc gccggaaaac
     1861 cgcgtcggct cgaagtttgt cgaagtgacc gtgatcggcc gcgacggcaa gctgctgcgc
     1921 tgaccgggtc agaccactgg ggtcagcttg gcgacgctca gcgccagcca cttcacgcca
     1981 tgccgcgtga agttgatctg cgcccgcgcg tcgtcgccgc tgccttcgag catcatgacc
     2041 ttgccttcac cgaacttgtt gtgaaacacg ttcatgccgt ttttcagccc gtgcgccggc
     2101 gcatcgcgct gcaccggcac cgccgggttg ggaaaccggt cgccgcccag gttttttgaa
     2161 gcaaaagcgg ccttggccca atcgccacgg gcgccaccag ctcctgaatt cgtagcaccc
     2221 caggcgccgc tgccgccgcc gtaggctgac gaaaagccct ggtttttcgg tgtgatccat
     2281 ttgagcgccg cctccggcaa ctcgtcgaag aagcggcttt tcaggttgta acgcgtctgg
     2341 ccgtgcagca tgcgcgtctg cgagtggctc aggtacaggc gctggcgcgc gcgtgtgatg
     2401 gc
//
LOCUS       DAIDFG010000397         2364 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_397_length_2364_cov_15.083153, whole genome shotgun sequence.
ACCESSION   DAIDFG010000397 DAIDFG010000000
VERSION     DAIDFG010000397.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_397_length_2364_cov_15.083153"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          341 a          758 c          828 g          437 t
ORIGIN      
        1 cccgaggggg ccgctgcgcc tgcggcccgg cgaagccggt tccgcggccc ctgcttgaaa
       61 aagtcaattg cccccgtgca cgtgcactgt gcgtagtaac tgactgccga catggctcaa
      121 acaagctcca ccgccgttcc gcaccatgtc gccatcgtga tggacggcaa cggccgctgg
      181 gccacgcggc gtttcctgcc gcgcatcgcc ggccacaagc agggggttgg cgcgctgaac
      241 cgctgcgtgc aggcctgcat ggcccgcggc atcggtgtgc tgaccgtgtt tgccttttct
      301 tccgagaact ggaaccggcc gccggacgaa gtctccggcc tcatggagct gctggcgctg
      361 tcgctggcca gggaagtgcc cgcgctcaag gccaacggcg tgcaactgca ttttgtcggc
      421 gaccggcagc gcctgtccga caagttcaag gccgggctgg ctcaggccga gcaggccacc
      481 gcagacaacc agcggctgat cctcaatgtg tgtttcaact acggtggccg ctgggacatc
      541 gcccaggcgg cacgcaggct ggcagccgag ggccgggaga tcaccgaaat ggcgctggac
      601 cgtgcgctgg cgctggccca tgtgccggac ccggacctcc tgatccgcac cggcggcgag
      661 ttgcgcatca gcaacttcct gctctggcag gcggcgtatt ccgaactgca tttttccgac
      721 aagctctggc ccgacttcga cgaggcggcg ctcgacgaag cgatcgcggt ctatcgcagc
      781 cgcgagcggc gttttggcca gacctccgcg caagtcgttt cgccaaaccc caaccccgac
      841 atgaaggctg cctgatgctc aagcaacgcg tgatcacggc gctggtgatg ctggccattc
      901 tgctacctgc gcttttttac cgcaccccgg tgccgtttgc cgtggtcgcc ctggtgctga
      961 tcgccgcggg tgcctgggag tgggcccgcc tgaacggata tggccaactc agcgcgctgg
     1021 tggccggttt tgcctgcgtg gtgctgtgtg gcatgtcctg ggacctgggc tggctcgatg
     1081 aaccgcagcc cttgctgtgg cggctggccg gcgcgggttg ggtgctggcg ggtgcctggc
     1141 tgctgcgcgg tggcgtggcc ggctggtcgc gcattccgcg caccgtgcgc ctggtggtcg
     1201 gggtgctggc gctctggctg gcctggctgg ccgtggcgca ggcgcgtttc atcggcatcg
     1261 agttcctgct gtcggtactt ctgctggtct gggttgccga catcggcgcc tatttcgccg
     1321 gcaaggcgct gggcgggcgc ttcagcaagg gcaagctggc cccgtcgatc agccccggca
     1381 aaagctggga gggtgtctgg ggcggcatgg cgggggtgct ggtgctgtgt atcacctggc
     1441 tgctgctcga cgccaccggt gagagtctgt atgcgcgcct ggctgcacag cacggcctcg
     1501 ccgtgacgct gctggccatg gtgttcctgg cggccctcag cgtggtgggt gatctggtcg
     1561 agtccctgat caagcgtagc gccggcgcca aggattcgag tggtttgttg ccgggccacg
     1621 gcggtgtgct ggatcgggtc gatgcactgt tgccggtgct gccgctggcc atgatgctgg
     1681 tgacagtctg aacgcacgcc gatgacaatg cagaaacaac gtgtcacggt gctgggttca
     1741 accggctcgg tcggggtcaa cacactggac gtgattgccg gccatcccga ccgcttcgag
     1801 gtgtttgcac tcagtgcggc cacgcagacc gagctgatgc tggcgcagtg cctgaggttc
     1861 aaaccgtcct acgccgtcat ggcaagcgcc ccgcacgccc gcatcctggc tcaaagtttg
     1921 aaggaaaacg ggctctcaac cgaggtcctg cttgcgccgg atgctcttga aatgatagcg
     1981 tcggatgagc gggtggacac cgtgatggcg gccatcgtgg gcgcagccgg gctggcgccc
     2041 tgcatggcgg ccgcacgcgc cggcaagcgc ctgctgctgg ccaacaagga ggcgctggtg
     2101 gtcggcggcg aggtgttcat gcaggccgtg cgcgagggtg gggcgcagtt gctgcccatc
     2161 gacagcgaac actcggcgat ttttcagtcc ctgccggaag acccggcggc ctggatggag
     2221 cgcatcgaca agatcatcct cacggcctcc ggcgggccgt ttcgcacccg ggcgccagcc
     2281 acgctgcgcg atgtcacccc cgacgaggcc tgcgcccacc cgaactgggt gatgggccgc
     2341 aagatttcgg tggactcggc cacc
//
LOCUS       DAIDFG010000398         2355 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_398_length_2355_cov_16.624348, whole genome shotgun sequence.
ACCESSION   DAIDFG010000398 DAIDFG010000000
VERSION     DAIDFG010000398.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_398_length_2355_cov_16.624348"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          433 a          780 c          740 g          402 t
ORIGIN      
        1 gcttgagggc atcgagccgg tggcccgagg tgtcaaaggc atagagccgg ccggtgttgc
       61 gcatgctggc gcccagggca agcgtcttgc cgcccgcgcc cgcgcaaaag tcgaccacca
      121 tttccccgcg tttggcgtca accagcaagg ccagcaactg cgagccctcg tcctgcacct
      181 cgatcgcgcc gcgggtgaag gcctccagct tgttgagcgc cggtttgccg gccagccgca
      241 ggccccaagg ggaatagggc gttgcgacag ccttgatgcc ggccttggcc agctccttct
      301 ggatgtctgc ccgcttgtct ttcagggtgt tgacccgcag gtccagccct gccggtgcat
      361 tgaggctttc caccagcggc cagaagtcgg ccccgagctg ggccttgagc ggctcgacca
      421 gccacgccgg caggttgtgg cggtgcggct ccatcaggtc gtccggtttg acctggtcgc
      481 agcgcgccag ccagtccttt tcctggtccg tcagggcgcc cagcagaaaa tcgcgctccg
      541 ccgcaaaacc cagaatggcc aggcgccgct cggtcgggcc gctgccgtgc tgggccaggt
      601 aggtgtagag attctttttg cgcaggacgc cgtaaatcgt ctcggccacg gtcgcgcgtt
      661 cgcgcgggcc gagacgctgc tcgcgaaaat ggcgcgacac caccatgtcg gcggggtgtt
      721 caaatctgag gacctgcttg agaagcgtgg cacaactgtc gagtaaggcg ttaggatgca
      781 tgcccctatt gtcccaccgc aggcccgcgg cgtgcctttt tgcatctgga tgaccatgtc
      841 tgacgacctt cccccgctga tcaccacccc gcccggcctg taccgccatt acaagggcat
      901 gctgtatgaa gtggtgggca ccgtgcgcca cagcgaaacc ctcgaacccc tgaccctgta
      961 ccgcgcgctt tatggcgagc acggcctgtg ggtgcgaccg gctgcaatgt tcaatgaaga
     1021 ggtggtgatc gacggcgtcc gccagccgcg tttcgccagg caagccagct agcccacccc
     1081 cgtcaacgat aatcgtgggt tatgtccgaa tccactgaaa tcgaaaaaca ggtcaagcgc
     1141 cgccgcacgt ttgccatcat ctcccaccct gacgccggca agaccacgct gacggaaaag
     1201 ctgctgctgt tctcgggcgc gatccagatc gccggcagcg tcaaggcgcg caaggccgca
     1261 cgccacgcga cctccgactg gatggagatc gaaaagcagc gcggcatctc ggtggccagc
     1321 tcggtcatgc agatggaata ccgcgactgc gtgatcaacc tgctggacac ccccggtcac
     1381 caggacttct ccgaagacac ctaccgcgtg ctgacggcgg tcgatgcggc gctgatggtg
     1441 attgacgcgg ccaacggcgt cgagccgcag acccggcgcc tgctgcaggt ctgccgcgcc
     1501 cgcaacacgc ccattctgac ctttgtcaac aagatggacc gcgaggtgca ggacccgatg
     1561 agcgtgatgg acgagatcga gcaggagctc ggcatgaccg tagtaccctt cacctggccg
     1621 gtcggcatgg gcaagctgtt ccacggtgtc tacgaccggc gtgaaaaacg catgcgtgtg
     1681 ttcagccccg gcgaagacaa ggccggcggc gacgatgaaa tcctggccgg cctggacaac
     1741 gccgaagcgg aacggcgttt tggcgccgag ttcacccagg cgcagggcga actggagttg
     1801 cttgaaggcg cctcacccga gtttgaccgt gccgcgtttc tctccggcaa gcagacaccg
     1861 atgtttttcg gctcggccat caacaacttc ggcgtgcagg aagtgctgga cgcgctggtg
     1921 gacctggcgc cggcgccggc cgatcgcacc gcgctgcagc gcgtggtgca tccggaagaa
     1981 aagaaattct cgggcgtggt tttcaagatc caggccaaca tggacccggc gcaccgcgac
     2041 cgcatcgcct ttttacgcgt ggccagcggc gagttcaccc gcggcatgcg cctgaaggtg
     2101 gtgcgcagcg gcaaggagat ccggcccaac accgtggtga gtttcatgag ccagcgccgc
     2161 gaactgctgg acaccgcctt tgccggcgac atcataggca tcccgaacca cggcatcctg
     2221 cagctcggcg acaccctcac cgaaggcgag gcgctgcaat tcaagggcat cccctacttc
     2281 gcccccgagc tattctctaa accgcgactg cgcgacccgt tgcgcgccaa gcagctcaac
     2341 aaaggcctgc tggag
//
LOCUS       DAIDFG010000399         2325 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_399_length_2325_cov_17.332599, whole genome shotgun sequence.
ACCESSION   DAIDFG010000399 DAIDFG010000000
VERSION     DAIDFG010000399.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_399_length_2325_cov_17.332599"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          529 a          738 c          622 g          436 t
ORIGIN      
        1 cgcagcggcc ccctcggggg gcagggagct acacgcagtg agcgaccgtg ggggcaatat
       61 tccggtggta tataatcatg ggcttcgcct tgctgcaccc ttaacgacgc tggggcagct
      121 tcaaccaaac cacaaggaga gtttatgcgt cattacgaga tcgtcttgct gatccacccg
      181 gatcaaagcg agcaagtccc agccatgctg gaacgttaca agggcatgat taccgccggc
      241 ggtggaaaag tgcatcgtgt tgaagattgg ggccgccgcc agctggtgta cctgatccaa
      301 aaactcgcca aagcccacta cctgtgcatc aacatcgaag ccagccaggc cgtgatggaa
      361 gaaattgaac acgcgttcaa gttcaatgac gccgtgttgc gccacctgac cgtggtcaag
      421 aaaaaggctg aaaccggccc gtccctcatg atgcgcaacg tcgagaggga agaggcccgc
      481 aaggcccagc agcaggagta tgcagcgtcg taagacgcca ccatgctgtt gaacgactca
      541 ctgctgcgca aaacaccctg cagtgaaccg cgttgaattg accgcttgta tcgccgaggc
      601 cagtcctctt cgctacaccc ccgctggaat ccccgctgcc aatttcgtcc tcgaacacga
      661 gtcggaaatg acagaagcag gttccacccg gcaggtcaaa ttgacaatga aggcagttgc
      721 ttttggtgcc ctggcagaac aaaccggcca gctccccttg ggcaaacctt tcaggttcac
      781 cggttttctg gtcaatgcgc gtggcagcaa aagtgtcgtg tttcatatcc aagcttttca
      841 accagtttct gaacaagtac tgaaccccat ttaaggagtc catcatgccc ggaccaaaac
      901 gtttcaacaa agacaagcgc cccaagcgca atacccaatc cctgctgttc aaacgcaagc
      961 gtttttgccg tttcacagct gctggtgttg aagaaatcga ctacaaggac atcgataccc
     1021 tgcgcgattt cgttgccgaa aacggcaaga tcatccccgc tcgcctgacc ggcacgcgcg
     1081 cgattttcca gcgccagatc aacaccgccg tcaagcgcgc tcgtttcctc gcgatgctgc
     1141 cttacagcga ccagcaccgc agcctgtaag gaacacgacc atgcaaatca ttctgatgga
     1201 caaagtcgtc aacctgggca atctgggtga agtcgtcaag gtcaaagacg gttacgcacg
     1261 caatttcctg atcccttccg gccgcgcccg tcgcgccacc gccatggcga tcaaggaatt
     1321 cgaagtcaaa cgcgccgaac ttgaaaaagc cgccgctgcc aagctggccg aaatgcaagc
     1381 ccagggcgaa aagctgggcg gtaccaccgt caagctcacg caaaaggcgg gcgtggacgg
     1441 ccgcctgttc ggctccgtca ccaaccatga catttcggcc gagctgacca agcagggctt
     1501 ccccgtgctc aagtcgcaga tccgcatgcc caacggtccc ctgaagaccg tcggcgacca
     1561 cccggtcacc gtggcgctgc acaccgatgt ggctgtggac atcaccgtga cggtgtacgg
     1621 cgaatccgct taaacagctt cactgctccg aaaaaagccg ctcgctgagc ggcttttttc
     1681 ttggcgttgt ttctcgatgg gcttgttcac acctacagcc ctgatttaca ccgcatttcc
     1741 cacgcttatt cacaacttta tccaccgcgt tttcgctgtg ccggcgtagt atttagcacc
     1801 ctctcaggaa tcccatgtcc gccgtacttt ccaccgctcc cgatccccgt tacagcgccg
     1861 accggcagat tgcccagctg cgcattccgc cgcactccat cgagggcgag tcgagtgttc
     1921 tgggcggact gctgctggac aacggcgcct gggaccgggt cggcgacctg ctggtcgacg
     1981 aggacttcta ccgctacgaa cacaaactga tctacgcggc cgtcggcgcc ctgatcaacg
     2041 ccaccaagcc ggccgacgtg atcacggtct acgaacacct gcagaaccaa ggcaaggccg
     2101 aggacatcgg cggcctgggt tacctgaact cgctggccca gtacgtgccc agcgcgggca
     2161 acatccgcag gtatgccgag atcgtgcgcg agcgctccat cctgcgcaag ctggtggccg
     2221 ccagcgacga aattgccacc catgccttca acccgcaggg caaggccgtg gccatgatcc
     2281 tggacgatgc cgagcagaag atcttcaaga tcggcgagca gggct
//
LOCUS       DAIDFG010000400         2310 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_400_length_2310_cov_24.674945, whole genome shotgun sequence.
ACCESSION   DAIDFG010000400 DAIDFG010000000
VERSION     DAIDFG010000400.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_400_length_2310_cov_24.674945"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          373 a          716 c          755 g          466 t
ORIGIN      
        1 gccgtcggcg ccggcctgca gcgcggccat caggtcttcc gcatgatccg acacggtcag
       61 catgatgatg cgcgaatcga ggccgtcgtc cttcagcgtt ttcagcacct cgacgccgtt
      121 catgtccttc atgttcaggt cgagcaggat catgtgcggc tgcaggcggt gggcgagtgc
      181 gatgccctcc ttgccgctgg cggcctcgcc gacgaaacgg aattcggggg cggcctggat
      241 cagctggatc acccccttgc ggaacagggg gtggtcgtcg acgatgagga tgctctgggg
      301 cgctgccggg ttcatggatg tggggtcagg tgacgggatg aatggggatg gtaacgtcgc
      361 gctcggcgct cggcatgaaa tgtagggtaa cgcgggtgcc tgacgtgggc aggttctcga
      421 ccgtcagccg gccgcccaga ttttttgccc gttcgtccat gatggccatg ccgtagtggt
      481 gcgcgccggc ggcctggcgg atgccgaggc cgtcgtcggt gacggtgacc gacaccgagc
      541 cgtcgctttt acaggtcacc ctgacctcgg cctttgtcgc cttggcatgg ttgagcacat
      601 tggacaaggc ttcgcggatg atctgcaggg catggatttc ctcgttggca ctgagcgtgc
      661 agccggccag atggacatcc agcgtgatcg ggatgccgcc gcggccggaa aactcggtca
      721 ccgtgttttg caaggcggca gccagccctt cgccctcgat gcgcaggcgg aaggtggtga
      781 gcagttcgcg cagctggcgg taggcgctgt tcaagccttc acgcaattcc gccagcactt
      841 ccctggcctc gtttgcccgc tgcggatcgt ccagcagcgg cttcatgcgg ctgacctgga
      901 ttttcatgta ggcaagcgac tgcgccagcg aatcgtgcag gtcgcgcgcc agcgcggcgc
      961 gttcttccag cagcgacacc cggcggcttt gttcgctgcg ccgcgcagtg ccgatggcga
     1021 tgccgatgtg gcgcgacagc gcttccagca actgggtctg ccacggtgcc agttccttgc
     1081 ccggcggcat ttcaagctgc agcacgccgt actggtgctc ggtgtccttt agcggcaaag
     1141 acagcacgcg ccggccatcg tgcagcggat gcacggccag cgtgtggtgc gccaaacact
     1201 cagcgcaact catcaggccg cacaggtcgg ggtcgccgct gtccgggtgc agcgtgctgg
     1261 cgatgacccg cgcgcgcgcg tcaccagatt ccaccaggca ggctcgcccg tgccccacgc
     1321 ccagcacgtt ttccagatcc ttcagcagga tggcgtaggt gtccggcgcc accggcccgt
     1381 tgtagagccg ggcgatcgag tggtagagca gttccagcga acggttggcg atggtgagtt
     1441 cggcggtctt ttccgtcacc cgcgcttcca ggttctggta cagcctggaa aggtcttccg
     1501 ccatgtggtt gaaagcctgg cccagccgtc cgatttcgtc ttccccgctc agatcggtgc
     1561 gcacggccag attgccctgc ccgatctggt cggcctgttc cagcagcctg cgcagcgggt
     1621 gaaccagaat attgttgacc aaatagatgg agaggatcac caccagcagg gtggcaatca
     1681 ccgatgcgcc gaggatgact cgcatgacca ggattttcgc ttcggcagcc tcttcgatct
     1741 gtttcaccag atcgttgatg tggtcgacga attcggggat cttgtccaac acagccgcgt
     1801 cagctacggt cgaggcggat gccgccggca ggctgaccag gcccggcttg atttgcgtgc
     1861 gccagctggt ttcgacctgc cggtggacct gccgcagcgg agtggttgca tcggccggca
     1921 gcacagccag gatggggccg gccttcagat cgccttcaaa ccgggtgatg gcctccgcga
     1981 gtgtctgcag gtgttcgggc gccctgttct gttgatagcg ctgataaatc gatgccatgc
     2041 gccagctctg cattcgcagg ctgcccgcca ggttgatcgc ctggccgttg ccctgggtgg
     2101 tttccgccac catccacgag gtggacatgc tggccaccgc cagcacggtg atggcgacaa
     2161 acatgccgcc cagccgcaac agcagggagt tctgcagctt attacccacc agacgaatca
     2221 cgtgtttgct ccatgcaatc ggcagtcgtt gccatgccgc tagcgtaata caccttctgt
     2281 cctgcattgt ggatcaaagg gcatacctct
//
LOCUS       DAIDFG010000401         2292 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_401_length_2292_cov_20.121591, whole genome shotgun sequence.
ACCESSION   DAIDFG010000401 DAIDFG010000000
VERSION     DAIDFG010000401.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_401_length_2292_cov_20.121591"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          434 a          742 c          769 g          347 t
ORIGIN      
        1 tgggcgacgc ggcccacccc atgtacccgc gcggctccaa cggctcggcc caggccctga
       61 tcgacgcgcg cgtgctggcc gaacagctgg cgctgcatgc cggtggcgat gcgcgggccg
      121 cgctgcaggc ttacgagcag ctgcggctga ccccgaccgc gaacatcgtg ctgacgaatc
      181 gctcggtgcc gcccgatttc atcaacatca aggtggacga gctgagcggc ggcaagcctt
      241 ttgcggacat cgatgcgctg atcagccagc aggaactgcg tgcgatttcg gaccactaca
      301 agcaggtggc cggcttctcg ctggaggcgg ccaggaatta ggctgcaatg gcgggcggcc
      361 ggagcgggcg ccgtccggaa accccggcac gtatcaattc aggtatttgg gccggggcag
      421 gtcaacggac agctcggcag gctcggtgtc gggcgtggcg tcctgcacca cgacgtcatt
      481 gggtgcggcc gccacggctg ccggcaccgt gagccactgc gagagcttca cgctctggtg
      541 cagcagatgc agcaggccgt tgctcagctg cgggtccatc atcacctgga aatcgcgcac
      601 cgtgggttgc ccgggcagct tttcaaacag ctgcaggcgc agctggccgt tgcccatcag
      661 ggtgaccttc acctcggtca gcagcaaggg ttcggggcct accgccacgt cggcgggcgg
      721 gccgggctgg tcgcggtaag gggtttcaaa atccccgctg cgcagcgtcg cttctttctg
      781 gaaggttgtc agcaactggc cgcgctgctc gtccaggctg gtggccgcct cctgcggggc
      841 cgccaccttg cgggtctggt cggccgcggt tttatcaagc acgggcagca gcgccagcgc
      901 caggcgccgg gtgagccagg cgcgcaactc gccgccgtcc cggccgttga tgcggatcat
      961 caggcggtcc tgctcctgga cgtaactgac cgacacctgg tgaatgttca tggtttcatt
     1021 ttagtccgcc gcctgttccc cgggggcttg atctccgtca agccgcaccc gggccaggct
     1081 gtgcataaag ggaagcgctt ccaaaggagc catcaacccg gttgtgagca ggggctattg
     1141 aaaagccccg ggttcagccc cagatcttca gcaatcaaat tcaccaaggg acgccatgcg
     1201 ccaggacaaa ctcaccacca aattccagga agcgctcagc gacgcacagt cgctcgcact
     1261 gggcaacgac aacgcctaca ttgagccggt gcacctgctg gccgccatgc tgggccagga
     1321 agacggcccc aaagcgctgc tgcagcgcgc gggtgtcaac gtggcggcct tgcaggccgc
     1381 tgctgacgcc gccgtcaaga agctgccgac ggtgtcgggc caggagcaga tccaggtcgg
     1441 ccccgagctc ggcaagctgc tgcaggccac cgaaaaggaa tccatcaagc gcggcgacca
     1501 gttcatcgcc ggcgagctgt atttgctggc agtggccgac agcaaggccg aggtcggcaa
     1561 gctcgccaaa gaggccggcc tgacacgcaa ggcgctggag gcggccatcg atgccgtgcg
     1621 cggcggccag ggcgtggaca gcccggaagc cgaaggccag cgcgaagcgc tcaagaaata
     1681 caccctggac ctgaccgagc gcgcccgcat gggcaagctg gacccggtca tcggccgcga
     1741 cgacgagatc cgccgcgcca tccaggtgct gcagcgccgc accaagaaca acccggtgct
     1801 gatcggcgag ccgggcgtcg gcaagaccgc catcgttgaa ggcctggccc agcgcatcgt
     1861 cgccggcgag gtgcccgatt cgctcaaggg caaacgtgtg ctgagcctgg acatggcggc
     1921 actgctggcc ggtgccaagt tccgcggcga gttcgaggaa cgcctgaaaa ccgtgctgaa
     1981 agaactggcc aaggacgaag gccagaccat cgtcttcatc gacgagctgc acaccatggt
     2041 gggcgccggc aaggccgaag gcgcgatgga cgcgggcaac atgctcaaac ctgcactggc
     2101 ccggggtgaa ttgcattgtg tgggtgcgac cacgctcgac gaataccgca agtacatcga
     2161 gaaagacgcc gcgctcgagc gccgcttcca gaaaatcctg gtgggcgagc cgacggtgga
     2221 agcgacgatt gccatcctgc gcggtctgca ggaaaaatac gaagttcacc atggcgtgca
     2281 gatcaccgac cc
//
LOCUS       DAIDFG010000402         2270 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_402_length_2270_cov_18.051467, whole genome shotgun sequence.
ACCESSION   DAIDFG010000402 DAIDFG010000000
VERSION     DAIDFG010000402.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_402_length_2270_cov_18.051467"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          435 a          741 c          684 g          410 t
ORIGIN      
        1 atcgccgccg cgcccggcct ggtggtgatc gacgaggcct accagccctt ttcgagcaaa
       61 agttacatcg accgcatcgg ccaacacagc cacgtgctgc tgatgcgcac gctgagcaag
      121 ttcggcctgg ccggcgtgcg gattggctac atgatgggcc cgaaagccct gattgccgaa
      181 atcgacaagg tgcgcccacc ctacaacatc agtgtgctca attacgagtg cgcgctgttt
      241 gcgctcgaac atgccgaggt atttggggcg caagcccagg aaatacgggc gcagcgtgct
      301 attcttttcg aagcgctgca ggctatggcg ggtgtccggg cctggaaaag cgatgccaac
      361 atgatcctgg tgcgtgtgcc cgatgcccag aaaaccttcg agggcatgcg ggcacgcaag
      421 gtgctggtca aaaacgtttc taaaatgcat ccattgctgg ccaactgcct gcgcctgacc
      481 gtcggcaccg ccgacgacaa tgtgcagatg ctggcagccc tcaaagaatc cctatgacca
      541 cccccaccgc catggcgccc cttgccccga gaacggccga agtttcgcgc aataccgccg
      601 aaacccagat caccgtcaag ctcaacctcg acggcaccgg ccaggccagg ctgtccaccg
      661 gcatcggttt cttcgaccac atgctggacc agatcgccag gcacggcctg atcgatctgg
      721 acatccaggc caagggcgac ctgcacatcg acgggcacca cacggtggaa gacgtgggca
      781 tcaccctggg gcaggccgtg gctcaggctg ttggcgacaa gaaaggcctg cgccgctacg
      841 gccatgccta tgtgccgctg gacgaggcgc tgtcgcgcgt ggtcatcgac ttttcaggac
      901 ggcccgggct ggacatgcat gtgcccttca agagcggcat gataggcgcg ttcgacagcc
      961 agctggccta cgagttcttc cagggctttg tgaaccacgc ctttgtgacc ctgcacatcg
     1021 acaacctcaa gggtgacaac gcgcaccacc aggccgaaac cgtcttcaag gcctttgcgc
     1081 gcgccctgcg catggcgctc gagatcgacc cccggtcggt cggcgtgatt ccctcgacca
     1141 agggctcgct gtaggcaaat ccttgtatgt ttttgccttc cagtccatgc tgtacttgct
     1201 cagacagcta taaaaacagt agcaaacttc cgtcctgatg gccaaattca agaaaatcgc
     1261 ggtggtggac tacggcatgg gcaacctgcg ctcggtgtcg caggcggtcc agcatgtcac
     1321 ggccgacagc gggttcgagg tgctcgtgac ctccagcgcg caggacgtgc tggacgccga
     1381 ccgcatcgtt ctgcccggcc agggcgcgat tgccgactgc atgcacgagc tgcaggcttc
     1441 gggcatgctc gaggcggtgt tgcaggcggc cgccagcaag ccgctgttcg gcgtctgtgt
     1501 cggcatgcag atgctgctgg accacagcgc ggaagggcgg ggcggtgagg gcacgcgtgg
     1561 cctgggcctg attccgggcg aggtgctcaa gtttgacctg gccgggcggc tgcagcccga
     1621 cggcagccgc tacaaggtgc cgcaaatggg ctggaaccag gtctaccagt cggtgccggc
     1681 gcatcccctg tgggcgggca tccccgatgg ctcctggttt tatttcgtgc acagctttta
     1741 cgcgcgcccg tcggatgcgc gccacaccgc gggcgaggcc gactacggcg cacgctttac
     1801 ggcggtggtt gcgcgcgata atattttcgc cacccagttt caccccgaaa aaagcgcaga
     1861 ccagggactg gccctgtacc gcaacttcct gcactggaat ccctgattca ctaccggcat
     1921 gcctgccccc gtcccagcca tctctgccgc accgctttcc tcacgtctca ctttcatcca
     1981 ccgttgcctc attgcccatg ctactcatcc ccgcgattga cttgaaagac ggccattgtg
     2041 tgcgcctcaa acagggcgac atggaccagt ccaccatctt cagcgaagac ccggccgcca
     2101 tggcgcgcag ctgggtcgac aagggcgcgc gccgcctgca cctggtggac ctgaacggcg
     2161 cctttgcagg caaaccaaaa aatgaagcgg ccattcgcaa gatcctgctg gaggtcggca
     2221 gcgagatcga cgtgcagctg ggcggcggca tccgcgacct cgacaccatc
//
LOCUS       DAIDFG010000403         2240 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_403_length_2240_cov_20.044851, whole genome shotgun sequence.
ACCESSION   DAIDFG010000403 DAIDFG010000000
VERSION     DAIDFG010000403.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_403_length_2240_cov_20.044851"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          395 a          686 c          730 g          429 t
ORIGIN      
        1 aacgacgtgg cgaccgtgca gcgtaccttg cagcgtacgg tggaggccac cgaggacgat
       61 ctgattgccc aggcgcgcca gacgcgcgaa ttgcagcgcg acctgttgcg cacccgcatg
      121 gtggagtttg aaggtatttc cgagcgcctg taccgcgtgg tgcgccaggc ctcgaaggaa
      181 accggcaagc aggtccggct ggatctgcag ggcggcacga tcgagatgga ccgcggcatg
      241 ctcgatcgca tgacgccggc gttcgaacac ttgctgcgta actgcgtggc ccacggcatt
      301 gaagatatgc aggcccgcac caacgcgggc aaggacccca gtggcctcat caccgtcagc
      361 ctgcagcagt cgggcaatga cgtgtcggtc gaattccgcg atgacggcgc cggtctcaat
      421 ctggagcgta tccgggagaa ggcgattgcc tccggcctgc tggctccggg ccagcagatc
      481 ggtgacgacg aagcggccaa catgattttc atgcccggct tcagtacggc gtcgcacgtg
      541 accgaacttt ccggccgggg tatcggtatg gacgtggtgc gctccgaggt caacgcactg
      601 ggcggccgca ttgaaaccag cactgttgcc ggcaagggca ccaacttcaa gctggtgctg
      661 cccctgacca cggcggtgac gcaggtggtg atgttgcgcg ctggcggctc ttccgtcggt
      721 gtgcccgcca acgtggttga aaccgtgcgc cggatttcgg cgaaggattt gcagcaggcc
      781 tacaacagcg gcgtgctcga tgtcgccggc gaagcggttc cgtttttctg gtcaggcgcc
      841 ttgctgcagg tctcccgcat gagcagcgag ccccagggca aaaccgcacc ggtggtcatt
      901 ttccgaagcg ccgcgcagcg gctggccctg cacgttgacg aggtgctggg caaccaggag
      961 gtggtggtta aaaacctcgg cccgcagctt tcccgcctgc cgggtctggc gggcatgtcg
     1021 gtgctggcct cgggcgccgt tgtcctgatt tacaaccccg ttgcactcgc atctgtttac
     1081 gggcagcagg cccgcgcctg gagttccgac agcgccgagc cgcacatgct cgagcaatcc
     1141 ggcgctggcg acgagcgcgg tttgtccgtc tcctcgcctg tcccagtgac accgcagatt
     1201 ccgctgattc tggtggtcga tgattcgatc acggtgcggc gcgtgaccca gcgtctgctg
     1261 cagcgtgagg gttatcgcgt ctccatggcc gcggacggct tgcaggcgct cgaacgcctg
     1321 caggaggagc gtcccacggt ggttctgtcc gacattgaaa tgccacgcat ggacggattt
     1381 gacctggcgc gcaacatccg cggcgatgcc cgtctgagcg atttgccgat catcatgatc
     1441 acctcgcgta ttgccgagaa gcaccgcgaa cacgccaagg aactcggggt tgaccactac
     1501 ctgggcaaac cttatgccga agacgagttg ctcagcctga ttcgccacta ttgcagcgca
     1561 gaagccgcag tttcataaag aaattggcgt ccagcccttt ctggacaagc gcttcaagct
     1621 atgaaaatag gagcgtttat tccgtggctg tttttttgac aggcgcgtag cgttgcaggg
     1681 tcagcgcccg cgcctcgtca tgctgcacga cgggaagcgg gtagttctct cccagccgga
     1741 cacccgcggc ggccagcgcc acgggcttgg ccagccaggg gctgtgcagg tcggtgccgg
     1801 tcagaccggc caactgcggc aggtacttgc ggatgaactt cccgtcggcg tcgaactttt
     1861 cgctctggct ggtcgggttg aagatgcgga agtagggttg cgcgtcgcag ccgctgctgg
     1921 cggcccattg ccagccgccg ttgttggctg acaagtcgaa gtcgttgagt ttttcggcga
     1981 aatatcgttc gccccagcgc cagtccagcc ccaagtcttt caccagaaaa ctggcggtga
     2041 ccatgcgcag gcgattgtgc atgtacccgg tctggttgat ctgggccatg gcggcatcga
     2101 ccagcgggta gccggtccgg ccttcacacc aggcccgaaa caatgcctcg gcagcaggcc
     2161 cctgttccca ggcaatggca tcgtacgcgg gtttgaagga acgctccgcc acatgcggga
     2221 agttgctcag gatctgcgca
//
LOCUS       DAIDFG010000404         2235 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_404_length_2235_cov_15.075688, whole genome shotgun sequence.
ACCESSION   DAIDFG010000404 DAIDFG010000000
VERSION     DAIDFG010000404.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_404_length_2235_cov_15.075688"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          347 a          732 c          745 g          411 t
ORIGIN      
        1 gatgccacgt cgccgccgcg cgcacgcgcc tgccagtcct gcacggtgtc cttgccgcgg
       61 gcctcggtca gcgcgcccag gcgttcgcag aaaaatgcga tgtccctgac aaaaaaatca
      121 tagtcctcga gcagcagcgc gacacgttcg tcttcggcga ggtccagctg caggcagggg
      181 gcggcgcgca tggcggcgat caggccttcg ggcacggcca cgttgccgac cttcttgctt
      241 tcgctctcga tgtagaccgg acgggccgga tcgaagctgc gcagcgcggc ccagatcagg
      301 ctgtcaaacg ccttctggct gggttgcggc acgccgggga tcaggcccag caccgagctg
      361 cggtggttcg ccagcgcctc caggtccagc acctgcgcgc cctgggcctg cagggcctgc
      421 agcagccggg tcttgccgct gccggtggtg ccgcacacca cctggtagct gtagcgggcg
      481 gcgagttgcg gcaggtcggc gaccagggct gcccggaagg ccttgtagcc gccgtccacc
      541 agggtgacct tgaagccgat ctggtccagg atcagtgcca gcgagccgct gcgtttgccg
      601 ccgcgccagc agtacaccag cggttgccat tcgcgcggtt tgtccagcac ctcgcgctgg
      661 atgtgggccg cgatgttttt ggcaaccagc gcggcgccca gctttttcgc ctcgaactgg
      721 ctgatctgct tgtaactcgt gccgacaatt ttgcgttcat cgtcgttcag gctgggccag
      781 ttgacggcgc cgggcaggtg atcctcggcg tattcgcctt cgctgcgggc gtcgatgatg
      841 gtcgaaaagt cggcgaggcg gaccagcgcc tcttgggccg tgatgcgatc gacgctcact
      901 tgagaagttt tcccagttcc ggccagacat tggccagcat cagcggatgc gcttgctcgt
      961 tggggtggat gcggtcggcc tggaacagcc gggtagcgtc ctcggtgtcg cccacaccct
     1021 tgagcaggaa aggcaccagc gccgccttgc ggtctttcgc gaccttggca aacagtccgg
     1081 aaaacttcgc tgcatagtcg ctgccgtagt tgggcggcac ctgcatgccc accagcagca
     1141 cccgggcgcc ggccttttgc gccgcctgcg tcatggcgcg caggttgcct tcggtcatgt
     1201 ccagcggcag gccgcgcagg gcgtcgttgc cgcccagctc gatgatgacc acgccgggct
     1261 tgtgctggct cagcagggcc ggcaggcgcg agcgcccgcc cgaggtggtg tcgccgctga
     1321 tgctggcgtt gaccacccgg gccggaattt tttcggcggc caggcgctgc tccatcagcc
     1381 tgacccagcc gctgccgcgt ttgaggccgt attcggcgct cagcgaatcg ccgaccacca
     1441 gaatcgtcgg gccggcgccg gctgctgcct gggccacggc ctgggcctgc ccgttctgcg
     1501 gcaataccag catgaaagtg cagagcatca ggcccagcag acccgcgcgc cgcccgggct
     1561 tggtaaagtc agggcgagtc attccaaaaa taattcccca aatatccgac aaggctggca
     1621 tgtttgaacc cgttgttcct gtttctccgg cgccgtccac cttcgcggca ggggcgggag
     1681 accgttcatc ttcaattatt tccgtcgagc atgttttcaa gagcgtgagc gactcgacgg
     1741 gcacgctgac cattttgcgc gatattgact tcgcactcgc ggccagggag actgccgcca
     1801 ttgtcggtgc ttccggttcg ggaaaaagca cgctgctgtc catcatcgcc gggctggaca
     1861 cgcccaccca gggcacggtg cggctggccg gccaggacct gtttgcgctc agcgaagacg
     1921 accgcgcggc gctgcgcgct cagaaggtcg gttttgtgtt ccagagtttt cagctcatgg
     1981 gcaacctcaa tgcgctggaa aacgtcatgc tgccgctgga actgtcgggc gcgaagaacg
     2041 cgcggtcgca ggccaccgag atgctcaagc gcgtcggcct cggtgaacgc ctgggccact
     2101 accccaaggt gctgtcgggc ggcgagcagc agcgggtggc gctggcccgg gcctttgtgg
     2161 tgcaaccggc ggtgctgctg gccgacgagc ccaccggcag cctggacttt gccaccggcg
     2221 aaaaagtcat ggcgc
//
LOCUS       DAIDFG010000405         2228 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_405_length_2228_cov_17.338242, whole genome shotgun sequence.
ACCESSION   DAIDFG010000405 DAIDFG010000000
VERSION     DAIDFG010000405.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_405_length_2228_cov_17.338242"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          444 a          765 c          700 g          319 t
ORIGIN      
        1 ccctggacac cggcatgggc ggcacgcccg ggaccacagg cgcggcaccg tcggccgact
       61 ggctgcactt gtacaccgaa gcctcgcgcc tggcctttgc cgaccgcgcg cagtacctgg
      121 gcgaccccga cttcgtgcaa gcgccgggcg gcaactggat gagcctgctg gacccggcct
      181 acctggccga acgcgcgaag ctgatcgcca cgcagcccgg cggacaaagc atgaaggtcg
      241 ccaaaccggg cacaccaggc ggcacgcgcc tggcttatgc ccccatgccc gagcagcccg
      301 aatacggcac ctcgcacatc agcatcgtcg atgcgtatgg caacgcgatt gcgatgacca
      361 ccacgatcga ggacgctttc ggcgcgcgcc agatggtcag gggtttcctg ctgaacaacg
      421 agctgaccga tttcagcttt gcgcccaccg atgccgatgg caagcccgtg gccaaccgcg
      481 tgcagccggg caaacggccg cgctcgtcga tggcgcccac cctggtgttc gacaaggcct
      541 ccggccagct cgtgatgagc ggcggcagcc cgggcggcgc gctgatcatc cactacaccg
      601 ccaagacgat ctatggcgtg ctgaactggg gaatgatgcc gcagcaggcc atcaacctgc
      661 ccaacttcgg ctcgctcaac ggcccgaccc tgctggaaga aaaacgcttt gcaccgccca
      721 ccgtcgaggc gctgaaagcc cgcggccatg aagtgcgcga gatgaccatg accagcggcc
      781 tgcaggcgat cacccgcggc caagcgcacg gcaagccgct gtggctggcc ggcgccgacc
      841 cgcgccgcga aggcgtggtc atgggagatt gagtataaaa agtggctgca gcccttctct
      901 agcgggcaca agcagctatt tttttggtag cgcgctggtt cgcatcggct cggccggcgc
      961 gtcctgcttg ccggtgccta aacgttcgta ttccgaaatc acctgcgcgc ccagcagcag
     1021 cagcgtcgcg gccagctcca ggctcagcag gacgatgatg gccgtggtca gcgagccgta
     1081 caccaggccg acctgcgaca gcgtgtcgaa gtaccagacc agcagatggc gcgtcacttc
     1141 ccacagcagc gccgccgtga tgccgcctat cagcgcgtgg cgcagcgaga gcttgccgac
     1201 cggcatcacc atgtagaccg aggtcagcac aaagatctcg ccggccaggc ccagcaggta
     1261 gagcaacacg cccgagaaac cgctgagcga ccagctgtag cccagaaact ccaggctctg
     1321 gtcgcccagg gcctggaagc tgccggagac cagcgtcacc agcagcaggc ccacgcccag
     1381 gcacaggatg tagacgtaag gcagcagcgc ggacaccagg aagcggcggc gccggatcgc
     1441 cacgcggtgc aggaaaatca ccgacatcgc gttttccagc acggtgaagg ccagggagct
     1501 gaaaaacagc atggtggcca gcagcaccca gcccatgagc tcgcgatggg cgagaaagtt
     1561 ttccagctcg cccacgatga agtcggcctg gcccggcgcc agccagccga tgtagcggcc
     1621 cagcgcctcc agcagcgccg actggtcgat cacatgcgac agcgcaatcg ccatcaggat
     1681 cagcagcgga acaatcgaca gcaaggcgta ataagccacc gcccccgcca gcagcaggcc
     1741 ctggttggcc cgaaagccca gcaacacgcg cagcgcaaac gcgccagggt gccggatcac
     1801 ataagcggcc tggggtccgg gggtcatggc tgcggcctgt tcaagatcaa ataagcctca
     1861 atcccttgcc agacatgcgc aagcagctat cttcttgata gcaaacccgc ctggcgtcat
     1921 acgtcgatcg cggcggccga gccggccagc ttgcgcagct cgaacttctg gatcttgccg
     1981 gtgctggtct tgggcagctc gccgaacacc accgcgcgcg gcaccttgaa gccggccagg
     2041 tgtttcttgc agtgcgccac gatgtcctcc ttggtcgccg tcgccccgac cttgagctcg
     2101 acaaaggcgc agggggtttc accccagcgt tcgtcgggct tggccaccac ggcggcggcc
     2161 agcacgtcgg ggtggcggta cagcacgtct tcgacctcga tcgaggagat gttttcgccg
     2221 ccggagat
//
LOCUS       DAIDFG010000406         2192 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_406_length_2192_cov_16.465138, whole genome shotgun sequence.
ACCESSION   DAIDFG010000406 DAIDFG010000000
VERSION     DAIDFG010000406.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_406_length_2192_cov_16.465138"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          396 a          692 c          667 g          437 t
ORIGIN      
        1 aaaccgcgca tgagcgacaa acgcgaactc aagctgatgc ggaccacggt gctgattttc
       61 agtgccctgg tactgggtta cgccatcttc atgcagggca cctcgattta cgacatggtg
      121 tcgggcgcct accaggtgcc cctggtcggc gcttttgtgc cgctggtctt tgggctgtac
      181 tggaaaaagg ccagcaccca gggcgccatt ttctcggtgg tttgtggcat cgcgacctgg
      241 ctgctgttta ccttcagtac cttgggggaa caattccctg cccagctggc gggtgtattg
      301 gccgcgacgc tgggcatgct ggtggggtcg ctgacgccgc agacgataga gaacagccgg
      361 ggcagccacc accggctgtc caccgaaggc tgaccggcgc cgccagcggc gccaggcgcc
      421 catcccgggc gggttgcggg tgccggcttc agaaagccgt agcgcctata attgaaggtt
      481 ttgcatcctg agggtcactt cgggacagca cccctctgac aaaacctctg aaaccatgcc
      541 aatctacgct tacaaatgcg agtcctgcgg gcataccaag gacgttttgc aaaaaatgtc
      601 cgatgccccg ctgaccgact gcccccagtg cggcgcgccc gctttcaaga agcagctcac
      661 cgcggccggc ttccagctca agggttcggg ctggtacgtg actgattttc gcggcggcgg
      721 cggtcccacc gcggttccgc ccgtcagcgg cggcgaagcc cctgctgctg ctgctgctgc
      781 tgcagacagc aagcccgccg agccgacagg gaaaacagcc gctgcccccg cggctgctcc
      841 cgcgccggcg ccggcggctt ccggtccaac caaggcgagt tgatgggcgc gatacgccga
      901 tggttgctgg ccgggttgct ggtcctggtg ccgctggcgg tcaccgtctg gctgctcaac
      961 tggatcattg gcacgctgga ccagacgctg caaattcttc ccggtgactg gcatcccgac
     1021 aaactgctgg gttttcacat tccgggtttt ggcgttctgc tggcgctggc catcgtgctg
     1081 tcgataggtg cgttggcgac caattttctc ggcaagaagc tggtcagctg gtgggatgcc
     1141 ctgcttagcc gcatccccat cgtccggtcc atttattcca gcgtcaagca ggtctccgac
     1201 accctgttct cggaaaacgg caatgcgttt cgcaaggcct tgctggtgca gtggccgcgc
     1261 gaaggtgtct ggaccatcgc gttccagacc ggcatgccgg gcggcgatgt ggccaaccat
     1321 ttgcaggggg actacctgag cgtgtatgtc ccgacgacgc ccaaccctac tggcggctat
     1381 ttcatcatgc tccccagggt cgactgcatt gaactcaaaa tgagtgttga cgaagccttg
     1441 acctatgtga tttccatggg tgtggtggtg cccggcagcc ccgccagcta caaaccgaaa
     1501 taatcttttc ttccagggcg tgcaagcgtc caccagtaca agcgatatat ctatgacctc
     1561 ccaaatgcgt tcccactatt gcggtctggt gaccgaagcc ctgatgggtc agactgtgag
     1621 cctgtgcggc tgggtcaatc gccggcgcga ccacggcggc gtgattttca tcgacctgcg
     1681 cgaccgcgag gggtatgtgc aggtggtgtg tgatcccgac cgcgccgaca tgttcaccac
     1741 ggccgaaggc gtgcgcaacg agttctgcgt gcagatcaag ggcctggtgc gcgcccgccc
     1801 ggacggcacg gtcaacgacg cgctgaaaag cggcaagatt gaagtgttgt gccacgaact
     1861 gatcgtgctc aaccccagcg tgacaccgcc cttccagctc gacgacgaca acctgtcgga
     1921 gaccacccgc ctcacgcacc gtgtgctgga cctgcgccgc ccctacatgc agaacaacct
     1981 gatgctgcgt taccgcgtgg ccatggaaac gcgcaagttc cttgatgcca acggcttcat
     2041 cgacatcgaa acgcccatgc tcaccaagag cacgccggaa ggcgcgcgcg actacctggt
     2101 gcccagccgc gtcaacgaag gcatgttttt tgcgctgccg cagagtccgc agctcttcaa
     2161 gcagctgctg atggtggccg gtttcgaccg tt
//
LOCUS       DAIDFG010000407         2188 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_407_length_2188_cov_16.945148, whole genome shotgun sequence.
ACCESSION   DAIDFG010000407 DAIDFG010000000
VERSION     DAIDFG010000407.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_407_length_2188_cov_16.945148"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          445 a          649 c          677 g          417 t
ORIGIN      
        1 ccagcgaatt ggccaccgcc atgccgaggt cgttgtggca atgcaccgac cagatggcct
       61 tgtcactgtt ggggacgcgc tcgcgcagcg tcttgatgaa attgccgtag agctcgggga
      121 cggcgtaacc gaccgtgtcg ggcacgttga tcgtggtcgc accttcggcg atcacggcct
      181 cgatgacgcg gcacaggaag tcgacatcgc tgcgatagcc gtcttccggg ctgaactcga
      241 catcggccac caggttgcgt gcaaaacgca ccgagagttt ggcctgttcc agcacctgct
      301 ccgggctcat gcgcagcttc ttttccatgt gcagggcact ggtggcaatg aaggtgtgga
      361 tgcgggcgct gttggcaccc ttgagcgctt cggccgcgcg ggaaatgtcc cggtcgttgg
      421 cacgcgaaag cgaacagatg gtcgagtcct tgatggcatt ggcaatcgcc tgcaccgcct
      481 cgaagtcgcc gttggaactg gcggcaaagc cggcctcgat cacatcgacc cgcaggcgct
      541 cgagctggcg ggcaatgcgc agtttttcat cgcgcgtcat cgaggcaccg ggcgactgct
      601 caccatcccg caaggtggtg tcgaaaatga tcaatttatc tgccatgctt gttctcctga
      661 gaggtcgatc tgatagtcaa aaaacgcaac ccaaaacaaa acggcccgct gcgtttgcat
      721 acgggccgtt tggaatcgcg cgcgtgcgct atcgcctgtg cccgtgtcgg tccagtagcg
      781 atagtgcaag cgaaattcgt ttcatggaaa tcaatgtacc acaaattgtg gccaggccca
      841 ggccggaggg aatggggcga cgcctacttg tgcagcccgc gctcatccgg ctcgtcggtt
      901 gaggtcgaga tcacgctggt ctgctgcccc ttggccttgc gccagccata cagcacgtag
      961 ccggaaaagc catagatgac gaacagaccg aacatgacgg tgggcggatc gatgttgatc
     1021 acggcgatgc ccagggcaat cagcacgatg acgacaaacg gaacgctgcg cttgagactc
     1081 acatccttga aactgtaaaa cggcacgttg gtgaccatgg tcaggcccga gtacagggtc
     1141 agcgcaaaca tgagccagcg aacttcctcg ccgcgcacgc cggcctcggt catcagccag
     1201 atgaagcccg cgaccagcgc ggccgccgcc ggtgaaggca ggccctggaa gaaacgcttg
     1261 tcgaccaccg ccgtgttgac gttgaaacgg gccagccgca gtgcggcgca ggcgcaatag
     1321 acaaaagccg caatccagcc ccagcggccc aggctcgtca gcgcccagac gtaggcaatc
     1381 aatgcgggcg ctgcgccaaa ggacaccatg tcggacagcg agtccatctg ctcgccgaac
     1441 gcgctctggg tgttggtcat gcgcgccacg cggccgtcca ggctgtcgag caccatggca
     1501 cagaacacac cgatggcggc ctggtcgaag cggccattca tggccatcac gatggcgtaa
     1561 aaaccgccaa acagcgctgc cagcgtgaac aggttgggca ggatgtaaat acccttgcgc
     1621 cgcttgcgcg gaacgagttc gtcggactcc tgcgcggcgt cgtgaccgtc atgcatgctg
     1681 ggtgctcccg gtgtcaggac cgttttcgcg ccaaccgcgc tgcgccgata gtgaatgatt
     1741 catgctgtga ggataccaaa cggaggccat cagaaataaa aaagccgcgc aaatgtcttg
     1801 cgcggcttga ggactggacc aaacggggcc ggtaagcccc gccgggtatc agttgcgggt
     1861 ctggtcaacc agcttgttct tggcaatcca gggcatcatg gcgcgcagtt tttcgcccac
     1921 gatctcgatc tggtgctccg aattcaggcg gcggcggctc aacagggttg gcgcaccggc
     1981 ctggttttcc agcaagaagc tcttggcata ttcgccggtc tggatgtcct tcaggcactt
     2041 gcgcatggct tctttcgtgg ccgaggtcac gatgttcggg ccggtcacgt attcgccata
     2101 ttcggcgttg ttggagatcg agtagttcat gttggcgatg ccgccttcat aaatcaggtc
     2161 aacgatcagc ttgagctcgt gcaggcac
//
LOCUS       DAIDFG010000408         2177 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_408_length_2177_cov_17.069746, whole genome shotgun sequence.
ACCESSION   DAIDFG010000408 DAIDFG010000000
VERSION     DAIDFG010000408.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_408_length_2177_cov_17.069746"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          431 a          741 c          623 g          382 t
ORIGIN      
        1 caggtctgca cactggcgcg aaacaggtcc atggggttgc gcttgtgcga ctccgtgtgt
       61 ttttccagcc gcacgttgat gtaggcggtc agccccagcc cgaccgactc gggcctgacc
      121 agcgccacat agcggtcgat cacgccgctt tcctccagcc gcttgacccg ccgcaacacg
      181 gcgctgggcg acaggctgac ctgctcggcg atctgttcgt aagtcgcccg gccgtcagct
      241 tgaaggctgc gcagtatgag cttgtccagc ttgtcaagtg cttccatagg tcatattttg
      301 caggtttact gcgcattcat caagtctggc gccgtgtttt gcataaaaat tgatgtgcta
      361 cacgtctaaa gtggatgtgc cgctattttt tctgaaccgc ctggagacaa cacgcatgaa
      421 cgcagccatc accaaacccg ccctgccttc cccgccagcc gccgcggctg cctgggacaa
      481 ccccatgggc acggacggct tcgagttcat tgaatacgcc gcgcccgacc ccgccgccat
      541 gggcgccctg ttcgaacgca tgggcttcaa gccgattgcc aaacaccgcc acaagaacgt
      601 gacgctgtat cgccagggcg gcatcaactt catcatcaac gccgagcccg actcctttgc
      661 ccagcgtttt gcgcgccagc acggccccag cgtctgtgcc atcgcctttc gcgtgcagga
      721 cgccaaagcc gcctatgaac gcgccatttc cctgggcgcc tggggttacg cccacaccgc
      781 cggccccggc gagctgaaca ttccggcgat caaaggcatt ggcgactcca tcatctattt
      841 catcgaccgc tggcgcggaa aaaacggcgc cagggaaggc gatatcggca acatcggttt
      901 tttcgatgtg gacttcgaac ccctgccggg cgccgaattg aaccccgtgg gccacggcct
      961 gacctatatc gaccacctga cccacaacgt gcaccgcggc cgcatggccg agtggtcggg
     1021 tttttacgag cgcctgttca acttccgcga ggtgcgttac ttcgacatcg agggtcaggc
     1081 caccggcgtc aagagcaagg ccatgaccag cccctgcggc aagatacgta tcccgatcaa
     1141 cgaggagggc aatgaaaaag ccggccagat ccaggaatac ctcgaccgtt accagggcga
     1201 aggcatccag cacattgcga tgggatccga cgacctgtac gccacggtgg acgcgctgca
     1261 gctgagcggc atcaagctgc tcaacaccag tgacacctac tacgagctgc tcgacaaacg
     1321 catacccggc cacggcgaaa acgtggccga gctgcgctcg cgcaacatcc tggtggacgg
     1381 cacgccgggc gagctgctgc tgcagatctt cagcgaaaac cagctcggtc cgatcttctt
     1441 cgagttcatc cagcgcaagg gcaaccaggg ttttggcgaa ggcaacttca aggcgctgtt
     1501 tgaaaccatg gagctcgacc agatgcgccg cggcgtgctg cagacagcat gacaaccacc
     1561 ctgtcacacg cagcaatcag cgatgacgca gcccgcgccg cgaagggccg ccccgagcaa
     1621 gcgacggccc cctcgggggg cggcgaacca cacgcagtgg ggagcgtggg ggctgctcca
     1681 cccgtcgttt acggcgccag cgaccgccca ccacgcggcg actacgcgcg cgccggcgca
     1741 gactacacct gcccgcagga ctacgacagc tataccgccg ctgatcacga cacctaccgt
     1801 cgcctgtacg agcgccaggc tgcgctgctg cccggcctgg cctgcgacga attcattgcc
     1861 gccctgccct cgctgggcgc cagcgaccac attccgcgtt ttgaagacat caaccagcgt
     1921 ctgcgcaaag ccaccggctg ggaaattgtc accgtgcccg ggctgattcc cgaggtgccc
     1981 tttttcacgc tgctggctaa ccacaagttt ccggtgaccg actggatacg caagccggaa
     2041 gagttcgact acatcgtcga gcctgacgtc ttccacgacc tgttcggcca tgtgccgctg
     2101 ctgttcaacc ccgtctttgc cgaccacatg caggaatacg gcaagggcgg gctcaaggcg
     2161 cacggcctgg gcgcctg
//
LOCUS       DAIDFG010000409         2134 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_409_length_2134_cov_16.190476, whole genome shotgun sequence.
ACCESSION   DAIDFG010000409 DAIDFG010000000
VERSION     DAIDFG010000409.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_409_length_2134_cov_16.190476"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          410 a          608 c          681 g          435 t
ORIGIN      
        1 gccgatgtag cgttgatctc ccggggcggc tacgggctga cacgcctgct gccgcatatc
       61 aactacaagg cgctggacaa ggcggtgtcc cggggcacac gttttgtcgg cctgagcgat
      121 ttcacggcgc tgcaaaacgc gctgctggcc aaaaccgggg cggtgacctg ggccggcccg
      181 gcactcggcg aggacttcgg gaccgaaggg cagcctgacg acatcatgga agcctgcttt
      241 gacgacctga tcacggggca gggcgagggc accggctggc gcctgccgaa aatcgttacg
      301 aaatcaatag ctgatggcgc ctggcagccg ggggccgagg gcaaatttgg cgtcaaaaat
      361 gcggttttgt ggggcggaaa cctcgcggtg ctgtgttcct tggtgggcac cccttacctg
      421 ccccaggtga agggcggcat cctgtttctg gaagaggtgc atgaacaccc gtaccggctg
      481 gagcgcatgt tgacccagct gttgcacgcc ggtgtgctgg cgcagcagaa ggcggtggtg
      541 ctggggcagt tcaccaacta caagctcgtg ccgcacgaca agggattcaa actggccaca
      601 gtggtggcgt ggttacagcg ccagatcaag gcgccggtgt tgagcgggtt gccgtttggc
      661 catgtgccga ccaaggtggt gttgccggtg ggggggaagg tgacgctggc cgtcgaggac
      721 agggacgctt ttctgctctg gggtcatagc cattaagcgg gggaaacaca ccaagctggc
      781 cggcggtgtt gtcggcctcc tggcaccttg cccggtggtg ggcagcctag ttggtcaggg
      841 aactgcgccg cgagccgagg ctgccgggca gcaggccttc aaacatcttg gtgatgccgc
      901 tgaccttctg gcgggcgatg gtttcgccct gcatggccga cagcacggcc atgacgtcac
      961 cgcgggcata ccagagaccc tgcgcatcct gggcgtagcg cacccgcagc gcaattttcg
     1021 ggtaatgttt ttcgccgaac tcgccgagtt cgttgagcat caactgccga atgccttcgg
     1081 tacgcttttc caggctggaa ggcgtcactt cgccaccatt gagcagaccc agcaaactgc
     1141 ttttcaaatc aggtttaatc cagcgcatag cgtttgtgct ttcgagagta gggcggtgcg
     1201 gaaccgtgcg gtctggtaga ttaagcctcc acggttacag atgcaagatg tatcacgtga
     1261 catggttaac gtaaaacaac aaataaatca aagtgttagt aaccaaatgt catgttgatt
     1321 ctcgggttgt agacaaaggt attacttttt gaatgttccg gatttggtca cgccccgaaa
     1381 atcattgtga ttttctttgt gatttattta cctctcgctt ccccgttttt tcacaaattt
     1441 tttctattta gagcttgata tgactaccta cccctcgcac tacatcaatg gccaatgggt
     1501 caaggcgcaa tcggcagaga ctctgccggt gtacgactcg agcaccgagg aagtcatggc
     1561 aaccgtgccg gccggcacag cgctggaagc cgaacgcgcc gtgctggccg cccgcgctgc
     1621 ttttgacgcc tgggctgcct tgcccgtcga aacacgcgcc gcctacctgg acaaggttgc
     1681 ggccggcctg aaggcccgca cggacgagat ggcgctgacc atcgctcgtg aagtcggcat
     1741 gccgctgaaa atggctcgcg cggtgcaggt gggcgggccc acctggcact ggggcaactt
     1801 tgccaggatc gcgcgcaatt tcgaatggga aaagaaggtc ggcaactcgc tggtggtgcg
     1861 cgagccgatc ggtgtggtgg gctgcatcac gccgtggaat ttcccgctca gccagatcac
     1921 cctgaaaatc gcgccggcgc tggccgccgg ctgcaccgtg gtgctcaagc ccagcgagat
     1981 cgcgcccgtc aatgccatga tcctggccga aatcattcac gaagccggcc tgccgcctgg
     2041 cgtgttcaac ctcgtcaacg gcatgggtcc tgtggtcggc gaagtgctgg cgacccaccc
     2101 cgaggtggac atggtcagct tcaccgggtc cacg
//
LOCUS       DAIDFG010000410         2103 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_410_length_2103_cov_17.893555, whole genome shotgun sequence.
ACCESSION   DAIDFG010000410 DAIDFG010000000
VERSION     DAIDFG010000410.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_410_length_2103_cov_17.893555"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          340 a          691 c          722 g          350 t
ORIGIN      
        1 gagtttgacc tttcgcgcgg cgcggcgtgc ctgcatcacc gagctggcaa ccacgaggcc
       61 gatgacctgg ccgatgtgct cgacggtatc ggtggcaaac accggttcgt catgcacaaa
      121 ggtggccagc agcgggtcgc ccggaatgtc gtctgccaga acgacaccgc gcacgccggg
      181 catggccagc gcggccgtgg catccacgcc ccgcagccgg ccgtgggcga cggtcgagag
      241 gatgggcgcc gcgtacaggg tgccgcgcgc ctccaggatg tcgtccacat aggttgcggc
      301 acctgccacc tgcgcgcggg cgctttcgtg tttgtgcgac ttgccggagg cgccacgggc
      361 gggcctgttc gctgcggcgg gggcggccac ggtcggcaca gggaccacgg gcacagccgg
      421 gttgtcggcg cggctgaccg ggccttcgtt gtggttcttt tcccgcgcgt tcatgcggat
      481 tcctccacga gcacaaagga ttccagattg atctgctgca aaccctggct ctccagccag
      541 aagcgctgca gcaggttgcc cagcacctcg ctgcggtagg cgccggaagc gcgcatgtcg
      601 gagatcggtg aaaactcggc gcgcagggtg gtgatggcct gctgcacggt ggcgctggtc
      661 cagggccggc tggtgaggtc ggcttcagtg agcaccgcgc gcaccggcgt ggccgccacg
      721 ccgccggcgc ctatagaggc tttcagcacc ttgccgtcct cgatctgcag gttcagtgcc
      781 aggcagacgg ccgagatgtc gtcgtcatac cgtttggaaa tcttgtagac ccggagcaac
      841 tcctgcccct caccccagcc ctctcccctg aggggcgagg gagcaggagc gcctggtttt
      901 cctccctctc ccgccgggag agggctgggg tgagggcgca ccggccgcgg caccttgatc
      961 caggccagca cctcgtcttt ggccatcaca ttcttgcggt agccggtgta gagctgctcc
     1021 agcggcagct cgcggtggcc gcgcaggctc atcagcacga cattcgcgcc gagcgcgatc
     1081 agcagcggca tcgagtcgcc gatcggcgaa ccgttggcca cgttgccgcc cagggtgccg
     1141 gcgttgcgca ccggcaggcc ggcaaaacga tgggcaaagg cctgcagctg cggccggtcg
     1201 gccaccagcg cggcaaaggc gtcggccagc gtgacggccg cgccgatggc gacgtggtgg
     1261 ccgtaatgct cgacccgccg gagttcctcc acccgtgtca cgtccagcac ctggccgaac
     1321 tgcatgtgca tcttggtcac ccagagtccg acgtcggtgc agccggcgac gagctgggcg
     1381 tcggggtggg cggcgcgggc ggccagcagc tccgccaggg tccgcggcgc gttataagat
     1441 aaatcggcct ccagcccttt ccgggtctgc gcaagcagct ccagttttga tagcaattcg
     1501 gcctcgttga cctgcacggc gggcagtgtt gccatctgct gcgcagcgtc aaaaataggc
     1561 cggtagccgg tacagcggca caggttgcct gacagatcct gctgcgccag ctcgcgggtg
     1621 atgggtgtac cgcaagcaac gtggttttgg tacatgccaa acaggctcat gacaaaaccg
     1681 ggggtgcaaa agccgcattg ggagccgtgg cactgcacca tcgcttcctg cgccgggtgc
     1741 agcgtgccgt cggcggcggc caggtcttcc accgtccaca gtgcctggcc atcaatcgaa
     1801 tgcgccagct tgatgcagct gttgaccgcc cggtaacgga gacgtccgcc ttcggcctcg
     1861 ccgaccacca ccgtgcaggc accgcagtcg ccttcgccgc agccttcctt ggtgccggtg
     1921 caggcgaggt cgtcgcgcag cagatcgagc agcgtgcggc tgggtggtac attggaaagc
     1981 gagacgattt ccccccgccg cacgaagcgg atcgggctga cggcaagggc cgccacgggc
     2041 tcgggagcag tttcaacaga catgggtttc agggtaaaac ctcaggccct aggcaacata
     2101 ctg
//
LOCUS       DAIDFG010000411         2066 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_411_length_2066_cov_16.068623, whole genome shotgun sequence.
ACCESSION   DAIDFG010000411 DAIDFG010000000
VERSION     DAIDFG010000411.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_411_length_2066_cov_16.068623"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          335 a          644 c          666 g          421 t
ORIGIN      
        1 gttcgcccag caccttgagc agccaggccg ccgtgacctc caggctgccg cccttttttt
       61 cgcgctgggc cgccacgttg agcgactcca gcgtgttgag caggcggcgg gcatcgccgt
      121 cggcataggc cacaagctgg tcggcggcgt caggcgctat gtttttgata gctggcagcg
      181 ctttctcggc aaggaccctg gcgatgattt gcttgagatc ttcttccccc aggggctgga
      241 gcacatacac cgccgcgcgt gacaacaagg ccgagttgac ttcgaacgag gggttctcgg
      301 tggtcgcacc gatgaaggtg aacaggccac tttccacatg cggcaggaac gcatcctgct
      361 ggctcttgtt gaagcggtgc acctcgtcga caaacacgat ggtgcgtttt ccgccctgcc
      421 cctgccagat gctggcctgc tcgaccgctt cacggatttc cttgacaccg cccagcacgg
      481 ccgagatggt gatgaagtgg gcgtcgaaac tgctggccat caggcgcgcg atggtggtct
      541 tgccgacgcc gggcgggccc cagaggatgc agctgtgggg ctggcccgat tcaaaggcaa
      601 tgcgcagcgg catgtcttcg ccgagcaggt gctgctggcc tatcacctca ccgaggtttt
      661 tcggacgcag gcgctcggcc agcggggcca tggcccgggc atccgcgtcc tgcccgaaag
      721 gggtcccgtc gagagcggcc atgtatcttc ttctgttttt ttattggcga atcacgtccg
      781 cgccggcagg aggcttgaac tggaatccgc tggcattcag ggccgggttg acctcgaact
      841 ggctgaaggt catcaccgaa cgctggccga agctgtcgag gatttccagc accgccagct
      901 cactgccctt gccggtgttc cggaaaccaa cggtgatgtt ttgcacctgg ccgtccttgg
      961 tcctgggtgt ggcctgcacc cactccagac cgtttttgtc gggttgcggc accagggtga
     1021 aatcggcctg cagcgccttg atgtctgccg cagccgcgat cacggccgcg ggcgtgccgc
     1081 tgagcacctg cgtgagcttg cgggcggtca cctggttgag gtccacgtcg tgcagccaca
     1141 gggtctgccc gtcggacacg atgctttgct caaacggctt tttgtagaca aacttgaacc
     1201 ggttgggccg caagaattca aagcccccgc tggaggtttt ggtcttggcg gtctgccctt
     1261 cgcgtgccgg gctggtcacc acctgggtga aaacggcgcg tccgcttctg gtgttcttga
     1321 caaaagcctc caggctttcc aggccgctgg cggtgctcac ttgcgcgctg gcagcgatgg
     1381 caaaaacggc caaagacgtc gccagtgcgg cccggaatgc gccgaccaga ggacggcggg
     1441 cggcaacggc agcgggaaat gagagcaaat gggtcatggc aaatggaggg gtaagccccg
     1501 ttgggtgaac agtgcttgtg ggactcaagc ggcgggcatt ggttgcctga acaatgtatc
     1561 gccccggtaa atgccccgct gtcgtcattc ggcgcgtgcg ggcaccagaa tttcgcgctg
     1621 tccgctgccc gacatggcgc tgatcatgcc cgctttctcc atttcttcga gcatgcgggc
     1681 agcccggttg taaccgatct tgaggtggcg ctgcaccagc gagatcgaag ccttgcggtt
     1741 tttcagcacg atttcgaccg cctggtcgta catcgggtcc ttgtcgccct cgctgccgcc
     1801 gttgccgtcg cccagcatgt cgccctcgcc gtcaaccgtg ccgccttcga gcacgccgtc
     1861 gatgtagttg ggctcgccct gctgcttcag gtaggcgacg acgcggtgca cctcgtcgtc
     1921 gctgacgaac gcgccatgca cgcgaaccgg aaaaccggtg ccgctgggca tgtacagcat
     1981 gtcgcccatg cccagcaggg cctccgcgcc catctggtcg aggatggtgc ggctgtcgat
     2041 cttgctgctg acctggaagg acagcc
//
LOCUS       DAIDFG010000412         2060 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_412_length_2060_cov_17.038404, whole genome shotgun sequence.
ACCESSION   DAIDFG010000412 DAIDFG010000000
VERSION     DAIDFG010000412.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_412_length_2060_cov_17.038404"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          399 a          688 c          664 g          309 t
ORIGIN      
        1 aagatgccca tgccgcccat gccgtcgagc ggcttgagga tgatgtcctg gtgttcggca
       61 tggaagcgct tgatgtcctc ggggtcgcgc gtcaccagcg tgggaccgat gaactgcggg
      121 aactccatga tcgcgagttt ttcaggatgg tcgcgcagcg cgcgcggctt gttgaagacc
      181 cgggcgccgt cgcgctcggc ctgctcgagc aggtgcgtgg cgtagaaata ctcgctgtca
      241 aacggcgggt ccttgcgcat caggatggcg tcgacgtctt tcagcgcgac ctggcgcgtg
      301 ccgcgctcgg tgaaccagtc cttggcgtcg ccggtcagcg tgatgtcgcg cacatgggcc
      361 atcacgggtg caccgcgctg ccacatcagg tcctgctgct cgcaggcgct gatctggtgg
      421 ccgcgccgct gcgcctcgcg catcatcgcg aaggtggtgt ccttgtagat cttgaaagag
      481 gccagcgggt cggcaatgaa gagaatgttc atgaacgggt caatctgtaa gggggtggtg
      541 actgctcagg cagcgcgctg ccggtattgt tgtacaaata gcgcgatcgc gccggccacc
      601 acgccccaga aggccgagcc gatgccggca atggccacgc ccgacagcgt gaccagaaaa
      661 gtgatcagcg cggcctcacg gtgcgattcg tcacgcacgg cctgcgccag gccgctgcct
      721 atcgtgccca gcaaggccag cccggcaatc gcgaccacca gctccttggg gaaagccgtc
      781 agcaaaccgg tcacggcagc gccgaaaatg ccgatcacga cataaatggc gccgcaggcc
      841 acggccgcgg tgtagcgctt gcgcgggtcc tcgtgcgctt cggggcccat gcagatggcg
      901 gccgtgatgg cgctgaggtt cagcgcaaag gcgccaaaag gcgcgagcag cagcgccgcc
      961 aggcccgtga gcgtgatgac cttggacacc gggatgccgc cgtccggcgc gtcaggcccg
     1021 taccccgcgg cgcgaatggc cgccacgccc ggcaggttct gcgaggccat ggtcaccacg
     1081 aacagcggca gagccaggct gacaaccgcc gccagcgtga actgcggcgc gacaaacacg
     1141 ggcatggcaa aagagaagtt catggctgtc caggccatct cgccgcgcgc ggcaacaaac
     1201 acaatcgcgc cgaacaaggt cagcaccacc gcataccgcg gcatcaggcg tttgcccagc
     1261 agatagatcg ccagcatcaa cagcaccagc ggcaaggcgg tttgcgccgc agcaaaagcc
     1321 tgcaggccaa aacgcgccag cacgccggcc agcagggccg acgcaatcgc catcgggatc
     1381 cggttcatga ccttttcgaa ccagcctgtg acgccgaaca gcgtgatcag ccccgcactg
     1441 accatgaagg ccgccaccgc ctcgcccatc gagaagccgc ccgccaaccc cgcagtggcc
     1501 agcaccgccg cgcccggcgt ggaccaggcc accatgaccg gctttttcag ccacagcgaa
     1561 ggaatcgccg aacacagccc catgccccag cccagcgccc acatccacga gctgatctgc
     1621 tcaggcgtgg cgttgaaggc ctgcgccgcc tggaaaacaa tcgccaccga actggtgaag
     1681 ccgacgagca cggccacgaa gcctgcggtg aaggcggaga ggttgaggtc tctgaagaac
     1741 tgcatgtgga gattttggca gggttgcgca aaacccatga ggcgtcacgc tgcgcctgcg
     1801 gtttgccttg gacagtagga cggactcccc actccctccc cccgctgggc gatggcggag
     1861 agcttcattt ggccgcgttt gctgctctca cgtgcaaaca tgacggccgc gtaccccgac
     1921 ctgacgcgct gcgcgcgtca ggtctccccg catccgaacc cgcatttgtt cccacccgtc
     1981 gggctgggcc gaggagcact ggcaaaagcg gatcagggct cgcgattgtc tgaggcgaag
     2041 ccgagttcga gcgagacccc
//
LOCUS       DAIDFG010000413         2052 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_413_length_2052_cov_17.362043, whole genome shotgun sequence.
ACCESSION   DAIDFG010000413 DAIDFG010000000
VERSION     DAIDFG010000413.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_413_length_2052_cov_17.362043"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          367 a          667 c          637 g          381 t
ORIGIN      
        1 gccgacaggt cgggcgtgta gaccacgccg cccttgacca caaaaatgtt ctcgcccgcg
       61 ccttcgctga cgaagcccga ggcgtccagc aacagcgctt cgtcgtaacc gtcgtccagc
      121 gcttccatgt tggcaagaat ggagttggtg tagttgctga ccgccttggc ctgcgtcatg
      181 gtgatgttga cgtggtggcg cgtgtagctg gaggttttca cgcggatgcc gcgtttcatg
      241 ccctcttcgc cgaggtaggc gccccaggcc caggccgcca ccatcaggtg aatggtattg
      301 cctttggggc tgacgcccag cctctgcgag ccgatccagg tcagcggccg caggtagcag
      361 ctttccagct tgttgtcgcg caccacggcc ttttgcgctt ccatgacctc ttccttgctg
      421 aaaggaattt tcatgcgcag gatcttggcg ctgttgaaca ggcgctcggt gtgttcttcc
      481 agccggaaga tggccgtgct gccgtcggcc gtcttgtagg cgcgcacgcc ttcgaaggcg
      541 ccgcagccgt aatgcagggt atggctcagg acgtggattt tggcgtcacg ccattcgacc
      601 atctggccat ccatccagat tttgccgtcg cggtcttcca tggagggtgc tactggggac
      661 atggtactga tcctgtgatg aatgtcgtga aagggttccc ggagtctggc ctgctgcagg
      721 cggggccgaa gaacctgccg aaaaacccga aacgctgatt ttacgggccg ggcgacgggc
      781 cggcggcggc cggcggcgaa tccgtcaccg gcggcctgaa aagttcatgg tgcgccagct
      841 tgccgccgac aaaggtgcag gtgacatgcg actggctacc atcggtccag cgaaacacct
      901 cgggctgggc gtccttgtcg gattgcagcg cgcccaggct tcgggtcatg gccaccacat
      961 gcagcagcgt catgcccggc ttgagcctgg cattgagcat gacggcgctg tcgacataac
     1021 cgatgggccg gttggccgcg cgcttgagca cctgcatcat gcgggtgaag tggagcagca
     1081 gccacatcat caggccgccg accgcagccg ctacaccggc ccagccgaaa ccgcggtaag
     1141 ccagcaccac cacggcaacg ccggcgatcg gaacaaaaat tttctggaag ttcatggcgc
     1201 tattttcgca gccaccggcc acctcccggc tgcggggagt gcgtacgagc ttccacacag
     1261 ccatggccac ttgttatttt caagaaaaat cggccttcag cccagatgga gagggcgcca
     1321 acagctactg atttaatagc gctccatccg ccgcaccgag gtgaatcccg ctacgcgagg
     1381 cttcgcacca cctcgatcgc ctgctccacg cgttcgaccg catggatggt caaaccttcg
     1441 atcggcttct tgggcgcatt ggccttgggc accacggcca cactgaagcc cagtttggcc
     1501 gcttccttga gccgctcctg gccgcgcggc gcgggccgca cctcgccggc caggccgacc
     1561 tcgccgaaag cgatgaagcc tttgggcaac ggcttgccgc gcaggctgga agtgatcgcc
     1621 agcatcaccg acagatcggc cgccggctcg ctgatgcgca caccgccgac cgcgttgaca
     1681 aatacgtcct ggtccatgca ggcaatgccg gcgtggcggt gcagcaccgc cagcagcatc
     1741 gccagccgat cccggtccag gcccaccgac aggcgacgcg ggctggggcc gccgctgtcg
     1801 accagcgcct ggatttccac cagcagcggg cgcgtgcctt cgagcgtgac cagcacacag
     1861 ctgcccggca ccggctcggc gtgctggctc agaaaaatcg cgctggggtt gctcacgccc
     1921 ttgaggccct tttcggtcat ggcgaacacg ccgatctcgt tgaccgcgcc gaagcggttc
     1981 ttgaacgcgc gcaccagacg aaaactcgaa tgcgtgtcgc cctcgaaata gagcacggta
     2041 tcgaccatgt gt
//
LOCUS       DAIDFG010000414         2043 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_414_length_2043_cov_17.016097, whole genome shotgun sequence.
ACCESSION   DAIDFG010000414 DAIDFG010000000
VERSION     DAIDFG010000414.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_414_length_2043_cov_17.016097"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          347 a          641 c          682 g          373 t
ORIGIN      
        1 ggctcagttc ttcgagcagc aggaaagtgc tgggccggtt gccggggaaa tgcttgtgcc
       61 cacctgtatc gtccttgccc agcatcagcg cctgggcctg ggccagcaca ttggcgagca
      121 gcttgtcatt gtggccaggc aagccatgga aggatttttt gaccgcgaca aactcgaccg
      181 gcaccacgtc cgtgccctga tgcagcatct ggaagtaggc gtgctgacca ttggtgccgg
      241 gctcgcccca gagcacgggc gcggtggcaa atggcagcgg ctggccccgc gcgtccacgc
      301 gttttccgtt gctctccatc tccagctgct ggagatacgc cggccagcgg cgcaaggcgc
      361 tgctgtaggg cgccagactg cggctggtga aacggtgaaa gttgcggtac cagacatcga
      421 gcaggcccag gcgcaccggc aggttctgcg ccagcggtgc tgtgcggaaa tggccgtcca
      481 tcgcgtgcgc accagccagc agcgcgcgaa agccctgcgc gccgatgctg atggccagcg
      541 gcaggccaat ggccgaccac agcgaataac gcccgcccac ccagtcccag aaaccaaacg
      601 tcgtgctgat accaaaggcc tgggccgctg cgacgttggt cgtcagtgcc gcgaaatgac
      661 gcgcgatgtc ctgtccgccc tgtgcctcga accattgcct ggccgacagg gcattggtca
      721 tggtttcggt ggtcgtgaag gtttttgacg cgatcaggaa cagggtgttc ttcggcctga
      781 gctgcgccaa cacgccggcg agctcctgcc cgtcgacgtt ggagacgaag tgaaagcgct
      841 tgccggggat gcgcaaggcg tcgagcgcca gcaccgccat ctgcggaccg aggtcggagc
      901 cacctatgcc gatgttgacg atgtcggtga tggccccgtc caggcgcacc tgctctgcgt
      961 aggccagcat ggcggccagg gtctcatgca cctggacgag cccgtttgct atattttcag
     1021 gagcggctgt cgcccgtccc tgctgggctg gagcctgatt tctcaataga aaatgcatgg
     1081 ccgcacggtc ttcgctgcgg ttgatcttct cgccggcaaa catcgcatcg cggtatggtt
     1141 ccacgccacg ctggcgcgcc aggtccagca gcaaggcctg ggtgggcgcg tcgatcaggt
     1201 ttttggagag gtcggcaaag acgtgaggcg cctccaggct gaatgcagcg aaccggccgg
     1261 gatcggccgc aaaggcctgg cgcagatcga accttttccc tgtcgcgtca aactgcgcct
     1321 gcagcgcagc ccaggccggg gtctggtggc aaggtggtcg gttcatcggc cggacctagg
     1381 cggccagcat cagctgttca agcttgaccg catcggccgc aaaggcgcgt atgccttcgg
     1441 ccagcttttc ggtggccatc gcatcctcgt tgagcgcgta gcggaaggtc acctcgtcaa
     1501 agccgacggc cggcaggtcc agcgcctgtg cggcctgggc gtccagcgcg cgcgccagtg
     1561 gtgcctcgct ggcggccagc tggcccaaca gctcggggct gatggtcagc aggtcgcagc
     1621 cggccagggc ggtgatctgc ccgatgttgc gaaaactcgc gcccatgatc tcggtcttga
     1681 tgccgagttt cttgtagtgg ttgtagatct ggcgcaccga ctgcacgccc gggtcgttgg
     1741 cgccggcatg ggcggcctcg tcccacgcgg cgcctgccga ttttttgtac cagtcgtaaa
     1801 tacgaccgac aaagggtgaa atcagctgca ccccggcctg gccgcaggcc accgcctgac
     1861 agaaggaaaa aagcagggtc aggttggtgt ggatgccccg gtcttcgagc cggcgcgcgg
     1921 cctggatgcc ctcccaggtg gccgccacct tgatcagcac gcggtcgatg tggatgcctt
     1981 cggcctcgta cagctcgatg atgcgttcgc cacgcgtcaa ggtggcgctg ctgtcgaagc
     2041 taa
//
LOCUS       DAIDFG010000415         2029 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_415_length_2029_cov_11.587639, whole genome shotgun sequence.
ACCESSION   DAIDFG010000415 DAIDFG010000000
VERSION     DAIDFG010000415.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_415_length_2029_cov_11.587639"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          385 a          595 c          556 g          493 t
ORIGIN      
        1 ggaaagaacc ttgcggtcga tctcgatgcc ggcttttttc agaccgttgg caaactggct
       61 gtaggtcatg ccgaatgagc ggctggcggc attgatacgc gcaatccaca actggcggaa
      121 cacacgtttt ttgttacggc ggtcacggta ggcatattgc ccggccttca tcaccgcttc
      181 tttggcgatg cgatagacat tgccgcggcg gccgcggaaa ccttttgcaa gggctaaaac
      241 ttttttgtgg cgggcgcgag ccgttacacc acgtttgacg cgaggcatgt gagtactcct
      301 tgttcgtcgt taattaaatg cccatgccgg gcagcatctg tgccatgtga cccatattgg
      361 tctcatggac agcaaccgca ccacgcaggt ggcgtttgtt cttggtggtc ttcttggtca
      421 ggatgtgacg tttgaaggct tggccgcgct tgacggtacc acctggacga acgcgaaaac
      481 gtttcttcgc gctgctcttg gtcttcattt tgggcatgta aatgctcctt ttcatttgtg
      541 ctcgtgaggc gctgcgaacc ttccgcagac ttgatggccc cgagccactt gtagtggcga
      601 gtgtttaatt cgccgcttgt tggcggcggg ctttgacacc tgccaccttt gcaaaggcag
      661 gatttccctg ccttcactgg acatgctttc gcatccccga aaaacctgcc ggtgaaacca
      721 cccgcaggtt gaactccttg tcgccgacag ccgccctcag gcgcctgtcg gtgcagccgg
      781 tgccgcttcc gcgggcgccg ctgcgggctt ggcagccacc ttcttcttgc ccggcgcgat
      841 catcatgacc atctgccggc cttcgagctt gggaaactgc tccaccagaa tcaaatcagc
      901 caactcgtcg cgaatacgcg ccagcagggc cagacccagt tcctggtgcg tgatttcgcg
      961 accgcgaaag cgcagggtga tcttgcactt gtcgccctct tccaaaaagc gcttgatatt
     1021 gcgcagtttg atgttgtagt caccatcgtc ggtgccgggc cggaacttga tttccttgac
     1081 ctcgatcacc ttctgcttgg atttcgcgtc cgcggccttc ttctgctcgg cgtacttgaa
     1141 tttgccgtag tccatcaatc tgcacaccgg cggtaccgcc gtgggagaga tctcaaccag
     1201 atccacgtca tattcaccgg cgaggcgcaa ggcttccatg atgctgacaa cacccagagg
     1261 ttcgttgtcc ggcccggtca gacgtacttc gggggccatg atttcacggt tcaagcgatg
     1321 cttgcgttct tcgcggtggc ggcggtcacg aaattcagta gcgatggttc taatccttta
     1381 acgtttgcta taaaagccat agcagcttgc gcccgcccag caagggcgac aggccaaaat
     1441 catcaacaaa tgtgtcagca atcaggcttt ttgggtaatg tcagaagcaa ttgtttgcgc
     1501 aaaagcttcg agtgacatga cgccaaggtc tttgttaccc cgggcgcgca cggcaactgc
     1561 gccggcttcc ttctccttgt cacctacgac aaggatgtaa ggcagctttt gcatggagtg
     1621 ctcccgtatt ttatacgtaa ttttctcgtt gcgcaggtca agattgaccc taagcccttg
     1681 attttgaagc cttttagcta tttcacgaca gtaatcggcc tgggcatcgg tgatattgag
     1741 caccgaaacc tgcaccggcg ccagccaggt aggcagtgcg ccggcatgtt cctcgatcag
     1801 aatcccgatg aatcgctcca ggctgccgac gatggcccgg tgcagcatca ccgggcggtg
     1861 tcgggcaccg tcctcgccca cgtattcggc atccagccgc tccggcatcg agaaatcaac
     1921 ctgcatggtg ccgcactgcc attgccggcc aatcgcgtct ttcagcgtgt actcgatctt
     1981 ggggccgtaa aacgcgccat cgccgggcgc gatttcgaac tcgcagccg
//
LOCUS       DAIDFG010000416         2024 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_416_length_2024_cov_18.267141, whole genome shotgun sequence.
ACCESSION   DAIDFG010000416 DAIDFG010000000
VERSION     DAIDFG010000416.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_416_length_2024_cov_18.267141"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          387 a          668 c          642 g          327 t
ORIGIN      
        1 cggactatgt gctgcacacg atgatccggg tcatgcgcgg cggcgaggag gtgaagatct
       61 ccaagcgcgc cggcagctac gtcaccctac gcgacctgat cgagtggacc agcaaggacg
      121 cggtgcgctt tttcctgctc agccgcaagc ccgacaccga gtatgtgttc gacatcgatc
      181 tggccctggc caagaacaac gaaaacccgg tgtattacgt gcaatatgcg catgcccgca
      241 tctgctccgt gctggcggcc tggggcggtg atgtcgccac gctcaagggc atggacctgt
      301 ccgcgctcga cagcccgcag gcgcaggccc tgatgctgct gatggccaag taccccgaca
      361 tgctggccaa cgccgcaacc gggtttgcgc cgcacgacgt ggcgttttac ctgcgcgagc
      421 tcgcggccag ctaccacagc tactacgatg ccgagcgcat tctggtcgac gacgaagtgg
      481 tcaagctggc gcgcctggcc cttgtcgccg ccaccgcgca ggtgttgcac aatggcctga
      541 aggtgctggg cgttgacgcc ccgcagaaga tgtaaaccag agcctgacac catgacgcgt
      601 cactctttca ccaagcaaac cgggcaaggc atgaacacac aacgaggcgg gaccctgctg
      661 gggctcatta ttggtgcgct gatcggcctg gccgctgccc tggcggtggc cgtctacatc
      721 accaaggtgc cggtgccttt catgaacaag agccagtcgc gcagccccga ccatgacgcg
      781 gccgaggcca agaaaaaccg ggactgggac cccaatgccc ccctgtatgg caaaaacccg
      841 gccaagccgt ctgcggcgtc gggcggaccg gtgatcgatg tcacgcccca gaagccggca
      901 gcggcgcctg tggccccggc tgagccggct gccaggaccg ccaaacccgc ggtcgacccg
      961 atcggggacc tggccaaggc caggctggcc gcaccggccg ccgcccccgg ggccgacccg
     1021 ttcacctatt ttgtgcaggt gggcgccttc cgcacccccg aggacgccga gcagcagcgc
     1081 gccaagatct cgctgatggg attggaggcc agggtgaccg aacgcgagca gtccggccgc
     1141 acggtgttcc gggtgcggct ggggcctttc gacaagaagg acgacgccga caaggccaag
     1201 gaacggcttg acggcagcgg catagaaacc gccctggttc gcgtccagcg ctgaactttc
     1261 tgccggcagg gtatccaacc ttctcgcacc ccgccagcac taacggagaa atgactgatg
     1321 caacgacgtg aattttcgat ggccaccgct tcggtggctg ccgccgcctc tctgggcgcc
     1381 ctgagtatcc cggcccaggc ccaggggccg gcgccgcgcg cgggaaccga cttcctggtc
     1441 ctggacaaac cggccccggt cgaggccccg gccggtaagg ttgaggtggt ggagttcttc
     1501 tggtacagct gcccgcactg caacactttt gaaccggcac tggacgagtg gatcaaaaag
     1561 gcgccgaaag acgtggtggt ccgccgcgtg ccgatcgcct tccgtgccga tttcgagccg
     1621 cagcagcgcc tttactacgt gctcgaggcc atgaacaagg tcgacgaact gcacaaaaag
     1681 gtgttttacg ccatccatgt ggaaaagcag acgctcaaaa cgccggaact gattgcggac
     1741 tgggctgaaa agcagggcct caacaaggcg aagtttctcg agacctacaa ctcgttttcg
     1801 gtggcgacca aggcccgcaa ggccacccag ctgcaggatg cctacaagat cgacggcgtt
     1861 ccggcgcttg gcgtcgccgg gcgttatttc acctctgggt cgatggcaca gaccatgggg
     1921 cgcgcgctgc tggtcactga ctacctgatt gcccaggtca ggaagggtta gcccccacgg
     1981 tcgctcactg cgtgtagctc cctgcccccc gagggggccg ctgc
//
LOCUS       DAIDFG010000417         1973 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_417_length_1973_cov_16.364964, whole genome shotgun sequence.
ACCESSION   DAIDFG010000417 DAIDFG010000000
VERSION     DAIDFG010000417.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_417_length_1973_cov_16.364964"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          423 a          598 c          586 g          366 t
ORIGIN      
        1 aagttttcct gctgacagct ttgttgccgc gagtggtcgc atcgatcatt tgcagcaatt
       61 cgaactggcc acgggtcagg ctttcgccca ggaggtattg caagggctgg aaaaacatgc
      121 gccgcaggcc aatgccgcca acagtttcgc attgctgctg atcgacggtt tgtccgtgcg
      181 cgaagagccg gtcacgcagg ccttgcaaaa tgccctggaa agccgcccgt tggtgggcgg
      241 ctcggcgggt gacggcatga atttcggcaa aacgcaggtg tatttcgagg ggcattttca
      301 cgcagacagc gccgtcctgg tgctggtcac cacgcctttg cccttccaga cattcaagat
      361 ccagcatgtc gttcccacgg accagcgggt cgtggtcacg caggccgatg ccgcacaaag
      421 gatagtcagg gaaatcgacg gctggccagc cgcgcaggct tatgcccggc tggtcggagt
      481 ggacgcgcag aatctggacc cgatgcgctt tgcagcgtcg ccggtggtgg tcgtgattga
      541 cggcacgaat tacgtacgct cgatccagaa ggccaacccc gacggcagcc tgactttttt
      601 ctgcgccatc gaggaaggtg tggtcctgcg tgtggcgcgc ggcgtggacc tggtcgagaa
      661 tctggagcac gccttcgccc ggatccaggc cgtcatcggc ccgccgcaat taattctcgg
      721 cttcgactgc atcctgcgca agctggaaat cgcccaaagc ggactggttg atcgtgtcga
      781 agcggtattc ctctccaaca aggtcatcgg ctttaacagc tacggggaac agtaccaggg
      841 cttccatgtc aaccagacgc tgaccggtat tgccatcggc gaggctgtca atggctgagc
      901 ccggcccacc tcccgccccg aatcagccgg acggaccgac cgaggtcgcc cggctcaaca
      961 agatcatcca ggcgttgatg gatcgcgccg aacgcaacac cagtgcccat gtgtccgatt
     1021 tcaccgcgtt ccagacaacg atcatgctga cggagcgggt acgccgccgc accgagcagc
     1081 tggaagcggc actgcgcgac aacgagaaag ccaaccgcat cctgcgcgca tccgaggaaa
     1141 aattccacgg actggcgaac caatctttga ccggcattgt cattgtcgaa gacgggaagt
     1201 tcagctactc caatggcaaa ttcaatgaaa tcttcggcta cagcgccgaa gaagtgcggc
     1261 aactgggacc gctcgatctc accatcgagt ctgatcgccc gctcgtggca gaacgttttc
     1321 gcaagctgct cggtggtgaa atgaaccaac ttaccctcac gatcgggggg ctgcgcaaga
     1381 atggcaccga agtggacatc gaagttcacg gcagtctcat ggcatccggc ggaacgctcc
     1441 aaaccattgc cgtgattctc gacatcagcg gtcgtcggca ggccgaacag gccctggcat
     1501 tcaagaacac catcctggcc acgcagcagg aggtttcgca ggacgcgatc ctggtggtcg
     1561 atgcgcaggg gaaaatcatt tcctacaacc gccgcttcat caatttatgg gcgcttgcgc
     1621 cagatgtggt ggcagctggc gtggacgagc cggtgctgca gcaggtcgtc gcccaggtgc
     1681 aggacccgga ggccttcctt gcccgcgtcg tctatctgta cgaacacgtc acagaggaaa
     1741 gccgcgagga actcgcactc aaggacggca gaatcctgga ccgctactcc gcgccaatga
     1801 caggcgcgga cggcacctac tatgggcggg tctggcattt ccgcgacatc accgaacgca
     1861 agcaggccga gaagcagatc cgcgatgaac gcgccttctc caacaagctg atcgccagcc
     1921 tgccggacat attctgcgtt ttcgacagca caggacgatt tatccactgg aac
//
LOCUS       DAIDFG010000418         1969 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_418_length_1969_cov_20.667712, whole genome shotgun sequence.
ACCESSION   DAIDFG010000418 DAIDFG010000000
VERSION     DAIDFG010000418.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_418_length_1969_cov_20.667712"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          380 a          622 c          663 g          304 t
ORIGIN      
        1 gcaggggcag ccacagcgtc atcagcagca gccagcacag cgtggcgccg ccagcgggca
       61 agaccatgga cttccacacc gccgcgcgct ggcggccggc ccgccacttg accagccagg
      121 cccaggccag ggtggccagc gcagcggcca aaaatgccag ccacgaaaag ctgggctcaa
      181 agccaggcgc cagcttggcg acgttcgcgg caggctgcct gggcatgccg gtctgcatgg
      241 caatccagat cacccagatg atgatggcgc agccggtgaa aaacagcagc gtgaaccagt
      301 caatcagcga ggccacgctg cgcttgaggg tgggcaaggc aaaggcggcc agtgccgcca
      361 gcggcggcag gctcagcagc aggctgcggt ccgacgcggt ggtggtcacc gtggcggcca
      421 gcgccaccag tgcaaaccac agcggcaggg ccacatgccg gctggtcagc tgccggcgcc
      481 agcgccacag ggtccacgcg gccagtggcc aggtgggcca ggcgaaccag atcagcagac
      541 gcgccacgct gcgccattcc ttgcgatctg ccgggctttc cagaccggga atttcgatgc
      601 gccagcgcca cagatccagc ccccaggaca gagcggccac cgccagggtc agacccccgg
      661 tcagcagcac ccagcgccag gcatgggccg ggttgttgtc gggccgggcg ctttgcgcgt
      721 tgccccatgc caccagcgtg ctgccggcac ccaggagcgc ggccaccgtg ggggcgccgc
      781 tcagggccag gccggccaag ccgaccacca gcccgagggc aggacccatc actttccagg
      841 cgttgcggta ggcgctggcc gccacggcat aaaaagccaa tgccgtgaaa ccgagctgcg
      901 ccagcgcggg tgtggtttcg tgggatagct gggccaggcc gaggctggcg atcagggcca
      961 gcagggcggc atcggcaatg gcccgggcgt aatcgaccgg gctggcttcc ccgccaaagg
     1021 caaacgccac gggctgggcc tgcgggctgc gcgccaggta atagacggcg taccaggtac
     1081 acagcagcgt cagcgccagc agcaggccga acggtatccg caccgcaaaa gcagggtcga
     1141 gaaagggcag ggccttgatc gccaatgcgc ccatccagta gggcgcgagc gcttcaaact
     1201 cgccgacctg ccccagcagc agcggatgca gccagctggt gttgccggca gccagttcgc
     1261 gcatggtgcc gaaggccgca atgtcggcgt ttttccaggg gccgcgaccg aggaagccgg
     1321 gcaggatata ggccacgcag aacagcagca gcgccagccg gggcaggcgg cgaactgcag
     1381 actgggcgat gatggctggc gagggtttgt tcaagggagg tcaggctgga cggaaggccc
     1441 gattttgggg caaaaaaaaa gcagcctgac attcaggctg ctttttcgtg tggccggagc
     1501 agttttgcag cttactcggc agcaggtgct gcagcgtctt tggcagcggt cttgccaaaa
     1561 cggttgcgga atttctcgac gcggccaccc atgttgtcca cggatttctg tgtgccggtg
     1621 taaaacggat gcgattcgct cgacgtatcg agtttgtaca gcggcagttc gcggccgtct
     1681 tccatcttca ccatttcttt ggtgttggcg caggaacggg tcacaaactt gaagttgttg
     1741 gacatgtcca ggaaacaaac ttcgcggtag ttggggtgaa tgccttcttt catgatcttt
     1801 ccttgttcgt ttcgggagcc actcaaacca gttctgagca ctttccgtga agcctcacat
     1861 tatgccatgg aatcaagcac ttacggggcc cccacgcttt ttcactgcgt gtaaacgctg
     1921 ccccccgagg gggcgctgcg cctgcggccc ggcaaagccg gttccgcgg
//
LOCUS       DAIDFG010000419         1961 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_419_length_1961_cov_16.223505, whole genome shotgun sequence.
ACCESSION   DAIDFG010000419 DAIDFG010000000
VERSION     DAIDFG010000419.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_419_length_1961_cov_16.223505"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          338 a          649 c          649 g          325 t
ORIGIN      
        1 cctattccca tgctgcctgg gccatcagct acaaattcga tagcgttgat ctgacgcttc
       61 ctgaaaaacc ccgtcatcgc ggccacgtca tacggcggcc ggtaacccag ccggacggtg
      121 atgccggtgt ccggcgagct ggccccctgg cgccgcatct gcgtcgggtt caggccgtag
      181 tggtcaacaa aggccgcatt gaagcgccgc acgctggcga agccgctgat cagcgcaatc
      241 tgcgtgaccg gcagggcggt gtcggccagc agctgcttgg cggtcagcag ccggcgcgtc
      301 tgcaggtact gcatgggcga cacaccgaat tgcgtctcga agatccgccg cacgtggcgg
      361 tcgctgatac ccagtttcgc ggccagcagt tcgacggaag gtgcagcttc cgtccaggcc
      421 tcgggctcat ctaataggcg cgccgcctgg tgcgccagga tgtaggaggc gtcctgaatc
      481 gaccagacca ccgagtgtgg cgccagttcg ggccggcagc gcaggcaggg ccggaagccc
      541 gcgttctctg cctgggcggc atggccgaag aagcggcagt tctcgcgttt gggcgtcttc
      601 acgctgcaga ccggccggca gtaaatgccc gtggaggtaa cacccgtaaa aaagcggccg
      661 tcaaagcggg cgtcccgcgc tttgagcgcg aggtagcagg cgtcgtcggc gagaggggtg
      721 gcttccgggt tcatgggctt gatgatacgt gccgggcggg ttttgactgg ccgtttccgg
      781 acatctcccg cgcgcccccg gcgcctacaa tcgacgctgt tttgcaaccc caggagtgcc
      841 taccccatgc agcttgacgc atccattttc aaggcttacg acatccgcgg cgtggtgccc
      901 tccaccgtca acgaaccggt tgccgaggcc ctgggccggg ctttcggcag catcgccctg
      961 gcccaggggg aacagacggt ggcggtgggg cgcgacgggc gcctgagcgg gccgtccctg
     1021 agcgcggcgc tgatgcgcgg cctggcggcc agcggcgtgg atgtggtcga tattggcatg
     1081 gccaccacgc ccatgcttta tttcgcggcc agcacgctgg ccagctccgg catccagatc
     1141 accggcagcc acaaccccaa agactacaac ggtttcaaga tggtgctggc cggccgcgcg
     1201 atttatggcg aggacatcca ggccctgcgc cgcagcatgg aagccgaagg ctgggccgca
     1261 ccggcccgcg tggggcaaat cacccaggtc aatgtgctcg cggcctaccg cgaccgcatc
     1321 gtcgccgaca tcaagctcac gcggccgatg aaaattgtca tcgactccgg caacggcatt
     1381 gccggggcgt cggcacccga catcttccgc gcgctgggct gcgaggtgac cgagctgttc
     1441 agcgaggtcg acggcacgtt tccgaatcac caccccgacc cgagcaagcc ggaaaacctc
     1501 aaggacctga tcaccgtgct gcaggccggc gacgccgagc tgggcctggc ctttgacggc
     1561 gacggcgacc ggctcggcat cgtcacgcgc gacggcaaca acatctaccc cgaccgccag
     1621 atgatgctgt ttgcgcgcga tgtgctatcg cgtgcgccgg gcggcgcgat cgtgttcgac
     1681 gtgaaatgtt cgcagcgcct ggcccccgag atcgaggccg ccggcggcgt gcccatgatg
     1741 taccgcaccg gtcactcgct gatcaaggcg aagatgaagg aagtcaattc gccgctgggc
     1801 ggcgagatga gtggccacat ctttttcaag gagcgctggt tcggttttga cgacggcacc
     1861 tacgccggcg cccgcctgct ggaaatcgtc agccgggcgc ccgacgccgg ggcgctgctc
     1921 aatgccctgc ccaccagctt cagcacgccc gaactcaacg t
//
LOCUS       DAIDFG010000420         1892 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_420_length_1892_cov_14.344039, whole genome shotgun sequence.
ACCESSION   DAIDFG010000420 DAIDFG010000000
VERSION     DAIDFG010000420.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_420_length_1892_cov_14.344039"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          316 a          613 c          604 g          359 t
ORIGIN      
        1 aaccggcttc gccgggccgc aggcgcagcg gccccctcgg ggggcagcgt agtacgcgca
       61 gcgacaagcg tgggggtcat tggggcagga agccttcaat cgacaggtaa cgctcaccgg
      121 tgtcgtagtt gaaacccagc accgtgctgc cggcaggaat ctccggcagt ttctgcgcga
      181 tggccgccag cgtggcccct gacgagatgc cgaccagcag gccctcttcg gtcgcgctgc
      241 ggcgggcaaa gtccttggcg gcatcgcctt cgacctggat cacgccgtcc agcaggctgg
      301 ttttcaggtt ggccggaatg aagcccgcgc ctatgccctg gatcgggtgc ggtgagggtt
      361 tgccgccgct gatcaccggc gaggccgagg gctccaccgc ataaaccttg gtgtttggaa
      421 atttggcctt caacacctgc gccacgccac tcaggtggcc gccggtgccg accccggtaa
      481 tcaggtagtc cacgccgtca ggaaagtccg ccaggatttc ctcggccgtg gtccttacat
      541 gcacttcgat gttggccggg ttttcaaact gctgtggcat ccaggcgcca agggtctgcg
      601 ataccagctc tttggcgcgg gcaatcgcgc cgttcatgcc tttttcgcgc ggcgtcagct
      661 cgaaactggc gccgtaagcc agcatcagcc ggcgccgctc gatgctcatg ctgtcgggca
      721 tcaccagcac cagtgtgtag cccttgaccg ccgcgaccat ggccagcccg acaccggtgt
      781 tgcccgaggt cggctcgatg atggtgccgc cgggcttcag cgcgcccgac tgctcggccg
      841 cttccaccat ggacagcgcg atgcggtcct tgatggagcc gcccgggttg ctgcgctcgg
      901 acttgatgta gatctggtgg gtctggcagg ccgcaccaaa aagccggttg atccggatgt
      961 ggggcgtatg accgatggtg gcgaggacgc tggcagcttt catggcgggt tccttgtgtt
     1021 tttgcccgga accggagcgg ttgcgcggac gcagggctgc tgcaaattat gtctcctccc
     1081 gacggggcgc gctgaacacg gaccgcccgc cggccgcacc cctgcgaagt gggggaaaat
     1141 cgcaactctt ggtaatttgc tgccggcccc caactttgct gttcagccgc atttttttgt
     1201 ttttctgctc tgtttttctg tcttcacggc cgtttccttc tggacccacc atgcgcaaat
     1261 tctggcttct gttttcccaa accgtgacgg ttttcctggc cgcctacttt gtcgtggcca
     1321 cgctcaagcc ccagtggctg aaccggccgg gcatgggcgc ggtggcggtg atcgaggcgc
     1381 cggccggcct gactggcgcc ccggcaccgg ccggcagctt tgccgcggcg gccaaggcgg
     1441 cttcggcggc ggtggtcagc atcaacacca gcaaggcggc agcgcgcaac ccgaacatgg
     1501 acgacccctg gttcaagttc ttctttggcg accagggggg gcaaaacgag ccgcaggtcg
     1561 ggctgggcag cggcgtgatc gtcagcccca acggttacat cctgaccaac aaccacgtgg
     1621 tcgaaggcgc ggacgacatc gaagtcatcc tcaacgacac gcgcaaggcc aaggccaaaa
     1681 tcataggcac cgaccctgaa accgacctgg cgatcctgaa aatcgaactc gacaagttgc
     1741 cggtcatcgt gctgggcagc tcggacaccc tgcaggtcgg cgacccggtg ctggccatcg
     1801 gcaacccctt cggcgtcggc cagacggtca ccagcggcat tgtcagcgcg ctgggccgca
     1861 accagctggg catcaacacc ttcgagaatt tc
//
LOCUS       DAIDFG010000421         1873 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_421_length_1873_cov_16.706821, whole genome shotgun sequence.
ACCESSION   DAIDFG010000421 DAIDFG010000000
VERSION     DAIDFG010000421.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_421_length_1873_cov_16.706821"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          296 a          620 c          604 g          353 t
ORIGIN      
        1 aatcaccgtc gggtcggtct gcagcatcat gccgatgcgc aggcggttgc tgaaaacccc
       61 accgatttcc gggcggtccg atggtttgcc ggtttccttt tccacaatgc tggccaggat
      121 cagggcttca tcgggcgttt tcaggggtgt gtccgggctg cgcagggccc aggccgcttc
      181 caggcgtttg tccatggcgc gggccgcgcg tttgaggacc gccaggtcgc tggagccctt
      241 ggcgtaggtg taggtgtcgg ggaaaaaccg gccttcggga tgcacaccgg gcttgcccag
      301 ctgcgccatg atggcctccg cgcccagccc ctgggtgtcg ggtttgaggg cttcggcttt
      361 ttgcagggcg gcgcgcacct ggcggaaatt ccagccctcg accagggtca cgcttttgag
      421 gctttcctcg ccgcgtacca gcatgctcag cagcatgcgc ggcgtggtgc ccggcgtgat
      481 ctcgtaactg ccggccttga tctgccgcgc ctggcccgag aggcgaaacc aggcatacag
      541 caacaccggg ggcacatcgg cgccgctggc ggccacggct tcggccacgc gctgggcggg
      601 ggtgccgggc tcgatctcca ggtccagcac ctggctgccg ggctgcggtt tcagggtcag
      661 gggttcgtgc agccaccagc cgacggcacc aaacaccacc agaccggcga caaacgccag
      721 cacgagcaaa ctcatcaaga aacgacgcac aaccctgtta ccttccggaa aacgatgggt
      781 ctatgataat tcagccatgt tgaccttccc ttccgattcc cttgctactt ccctgccatt
      841 gaccaccgtg ccgtccggcg ttgccgaact gacgcacctg ggtgtgatcc gtgtcgccgg
      901 tgaagaggcc gccaaattcc ttcagggcca gctcacgcag gacgtggcgc tgctgggcct
      961 gtccgaagcg cgcctggctg ctttctgcaa tgccaagggg cgcatgcagg ccagcttcat
     1021 cgttttcaaa cgcagcgagg gcgagatcct gctggtctgc agccgcgaca tcctgcccgc
     1081 caccctcaag cgcctgtcca tgtttgtgct gcgtgccaaa gccaggttga gcgatgccac
     1141 ggcggagtac accctctgcg gactggcggg tgatgctata gaaaccatag ctggcggcgc
     1201 acatcccgtt tgggccaagt ttgattttga tgctgcaaac ctggtctttc tttatcctgg
     1261 cgccggccag ccgcgtgcct tgtggtgcgc tccggccggc accccggcgc ctgcgggcgc
     1321 ggcgttgccg ctggcgcact ggcactggct cgaggtgcag tcgggcatcg ccatgatcac
     1381 acaaccgatt ttcgaagcct ttgtgccgca gatgctcaac tacgaatcgg tcggcggcgt
     1441 gagtttcaag aaaggctgtt accccggcca ggaaatcgtg gcgcgcagcc agttccgcgg
     1501 cacgctcaag cgccgcgcct acgtagcgca caccgacggc acggcacagg tgggccagga
     1561 ggtgtttcat gccgccgacg cagaacaacc ctgcggcctg gtcgcggcct gcgcagcgca
     1621 cccggcggcg ggttttgatg tgatcgtctc cctgcagacc gccgctgcgg ccgatgccgg
     1681 aggcgggcgg ctgactttag gcagtgcggc cgggccagcg ctggttttgc tgcctttgcc
     1741 ttacccgctg ctggaagata tctagtgcgc aacgcgcggc cgctccaagc aaaaccagcc
     1801 gctccccttc ctcttcatca gccggggccg cggaaccggc tttgccgggc cgcaggcgca
     1861 gcgccccctc ggg
//
LOCUS       DAIDFG010000422         1828 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_422_length_1828_cov_18.661027, whole genome shotgun sequence.
ACCESSION   DAIDFG010000422 DAIDFG010000000
VERSION     DAIDFG010000422.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_422_length_1828_cov_18.661027"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          318 a          556 c          574 g          380 t
ORIGIN      
        1 gcgcttgacc accagcatgc cgccggaacc gttgggcacg tccttgccga gttcatcgac
       61 gatggccgcc atgatgccgg gcagcggtag cgtgcagctg ccagggacca gcggcgtcgc
      121 gcccggcagc ggcgtgatca tgtggccgcc ggtttcggtc tgccagaagg tgtcaaccac
      181 cgggcagcgc tcgccaccga cgttcttgta gtaccacatc caggcttcgg ggttgatggg
      241 ctcgcccacc gtgccgagaa tgcgcaggct gctcaggtca gagcgcgcag gatggatttt
      301 ttcgtcgccc tcggccgcct tgatcaacga gcggatcgcg gtcggtgccg tgtagaaaat
      361 gctgaccttg tgtttttcga tcatctgcca gaaccggccg gcattcgggt aagtggggat
      421 gccctcaaaa atcacctgcg tcgcgccagc ggccagcggg ccgtaagcca cataggtgtg
      481 gccggtgatc cagccgatgt cggcggtgca ccagaacaca tcggacggct ggatgtcgaa
      541 ggtccagtcc atggtcagct tggcccacag cagataaccg ccggtgctgt gctgcacacc
      601 cttgggtttg ccggtggagc cggaggtgta gagaatgaac agcggatgtt cggcaccgac
      661 gaattccggc tcgcaggtgg ccgattgctt tgccgtgacc tcgtgcatca ggctgtcgcg
      721 cccggccacc atggcgcagg ccgtggccgt gcgctggtag acgatgacgt tcttgatcga
      781 ctcgcagccg cccatggcga tgccctcgtc cacgatggcc ttgagcggca gctccttgcc
      841 gccgcgcagc tggtagttgg cggtgatgac cgcgaccgcg cctgcgtcct ggatgcgttc
      901 ctgcaggctc ttggcggaaa agccgccgaa caccaccgag tgggtcgcgc ctatgcgtgc
      961 gcaggcctgc atggcgacca cgccttcgac cgtcatgggc atgtagatga tgacgcggtc
     1021 gcccttcttg atgcccatgg ccttgagcgc gttggcaaac tggcagacct tgccgtgcaa
     1081 ttccttgtag gtgacgttgg tcaccttgcc gtcgtcgctc tcgaagatga tggccttctt
     1141 gttctcggtg gcggtgccga ggtggcggtc aaggcagttg taggaggcgt tgagctcgcc
     1201 gtcggcaaac cattggtaga acggcgcatt cgactcgtcc agcgtctggg tgaagggttt
     1261 cttccagctg aggttctcgc gcgccagccg ggcccagaaa ccctcgaagt ctttttcggc
     1321 ttcggcgcac agcgcctggt aggcgtccat gccggaaatg cgggcggatc tggccagctc
     1381 ggggttgggg aaaaagaccc ggttttcaac cagtgtggat tcgatggcgg atgaaggcgc
     1441 gttcatgatg gcttgtctcc tcaggttgtc gttgatacgt gctaatgtca tatttggcac
     1501 ttacagtcca ctgactggct ggaagcttac agatttaacc cgaattcagg ccgggcgggc
     1561 agcacgccta caatccgcac agtccgtaca aacccccata aaaatcatgt cagacaccaa
     1621 gcccgtcatc accaaagttt ccccgctgcg cccgattccc aacttcattt ttgccagccg
     1681 ctggctgcag ctacccctgt atgtcggcct gatcgccgcg cagggtgtct atgtcttcca
     1741 cttcctggtc gagctgctgc acctgatcga ggccgtcttc ggcagccaaa ccgcgctaca
     1801 ggccctggtg accagcatag gctacaaa
//
LOCUS       DAIDFG010000423         1828 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_423_length_1828_cov_16.507614, whole genome shotgun sequence.
ACCESSION   DAIDFG010000423 DAIDFG010000000
VERSION     DAIDFG010000423.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_423_length_1828_cov_16.507614"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          275 a          584 c          629 g          340 t
ORIGIN      
        1 caagtccgac ctgttcgagg tcatgatggc ggccacctcg ggcaggctcg acgaggtgga
       61 gctggaatgg gaccgccgcg tggcgctggg cgtggtcatg gccgcgcacg gctacccgct
      121 acagccccgc aagggcgacc tgatcaccgg tctgccggtg gtgcatccgt ccgaggcgcc
      181 ggaagatgtg gtggtgttcc atgccggcac cgcgacggct gcgggcggta tcaaggccgg
      241 tgtggtgacc tccggcggtc gtgtgttgtg tgtcacggcg ctggccagct ccgtgcgcga
      301 ggcccagcac ctggcctacg aggcaacgca ggcgattcat ttcgacggcg cccagtaccg
      361 cagggacatc ggattccggg ccatcaagtc ctgatgggca tggatttcca gccgccgcat
      421 cccgtgcagt tccaggggag cgggctggcc cgggcggtgg tcagggccct cggctggcgc
      481 gtggattttg ccggcctgcc gacgctgcag ggcgtgattg tggtgtaccc gcacacctcg
      541 aactgggatt tccccatcgc cgtgctgctc aagtgggcgg tcggcattcc ggtgcagttc
      601 tggggcaagg acacgctgtt tctcattccg ctgttcggat cgtggctgcg ctggctgggc
      661 ggcgtgcctg tcaggcgctc ggcaccccag ggcgtggtcg ggcagatgac ggccatgatg
      721 aaacagcaaa aggccgacgg gcagtacttc tggctggccc tgtcgccgga aggaacacgc
      781 cggcttaccg caggctggcg cagcgggttt taccggctgg cgctggaagc cgatgtgccg
      841 gtgggcctgg ccggactcga ctattcacgc aaacgcctgg tctttcgcga ttttgtgcgg
      901 ctgaccggcg atgaagaccg cgacctggag cgcatggcca agtccctgca ggacttccgc
      961 ggctttcggg ccggcgccgc ggcccccgtg cgtttgatca attcaggcct gccccaggcc
     1021 gataccatcg tgaaatgacc accatgaccg attcttccca tgtccgcagc tacttgctgg
     1081 agttgcagga gcgcatcatc agcaccgtcg ccgcactcga cggccagccc tttgtggccg
     1141 acccctggca caaggacccc ggcgaaacgc tgcagggcga cggcatcacc cggatcctgg
     1201 agcagggcag ggtgtttgaa cgcgccggct gcggcttctc gcatgtgcgc ggccccaggc
     1261 tgccaccttc ggccacgcag caccggcccg agctgtccgg cgcgcccttc gaggccatgg
     1321 gtgtgtcgct ggtgtttcac ccgcgcaacc cctatgtgcc taccgtgcac atgaatgtgc
     1381 gcatgatcgc ggcgactccg gcctcgggcc cgacggccta ctggttcggc ggcggcatgg
     1441 atttgacgcc gtattacggg tttgaggagg atgcggtgca tttccaccag acctgcaagg
     1501 acgccctggg cccgtttggc gaggacaagt acccgcgctt caagacctgg tgcgacgagt
     1561 atttctacct gaagcaccgc gacgagcagc gcggcatagg cggcgtgttt ttcgacgatt
     1621 tccaggaact cggcttcgag ggcagcctgg cgatgatgcg ttcggtcggc gacgccttcc
     1681 tggccgccta cctgcccatt gtcgagcgcc gcaaggacat ggcttacggc gagcgcgagc
     1741 gcgatttcca gctgtatcgc cgcggccgtt atgtggagtt caacctggtc tgggaccgcg
     1801 gcacccactt tggcctgcag tcgggcgg
//
LOCUS       DAIDFG010000424         1822 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_424_length_1822_cov_22.204867, whole genome shotgun sequence.
ACCESSION   DAIDFG010000424 DAIDFG010000000
VERSION     DAIDFG010000424.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_424_length_1822_cov_22.204867"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          378 a          572 c          533 g          339 t
ORIGIN      
        1 gtcaggccac gcacccgatt gagttcgaca tgtactattc actgtaattt tttacagtct
       61 gtggtcaaaa aaggacggcg aaagccgtcc ttttttattg ggctgcatgg ttatctggta
      121 gcccattttg cttttcgaca cctttgcgag atactggtag accgtccttg ccctaacagg
      181 cgcttggggt tacgcatgaa acctacagta cttctttcaa tctcgaccct tcttctcgct
      241 ggcagcgtct ccgccgcatt cgcgcaggag cgcatctacc gctgcggtgg caaggaaggc
      301 gctgcgcctg aatacatcaa caacgccagg gatgcgcagt cgcggggctg caagcaaatt
      361 gaaggcggca atgtgacggt ggtccagagc ctgccccaga gccggcctgc ggtacgtgtt
      421 gctgccgcag cgcaggcatc gcctgccgcc agcaacgaag cgcaacgcgc ccgggacagc
      481 gacacacgcg ccatcctgga gtccgaactc aaaaaggccg agctccggct cgccgagcag
      541 caaaaagagt acaacaacgg tcagcccgag aagcagggca tcgaagggcg caattaccag
      601 cgctacctgg accgtgtggc tgaaatgaag gaaagcattg cccgccacga aagcgacatc
      661 gccggtctga agcgcgaaat ttcccgtttg cccgcaaaca ccgtccgtca gtaagacgtt
      721 ctgcgtcaga atagaaggct tgaaaaagcc cactctttcc gcgcccgcgg ccgctccggc
      781 cattccccgt ttccagtctt ttgacctgct ggccacactg gtcgccgttg tgcgcaccaa
      841 cggtttggtg gtgtttgcca acgcggcgct cgaagacgcg ctgggcacct cgcgccgtac
      901 catcgaaggc tcccaactcc ccgattgctt caccgagccg gccatcctgc acaacgcact
      961 cgaaggcgcc ggcagcaatg aatttgccgc gctgcgttac gacgcctggc tcaagccgct
     1021 gaaccgcgaa cccatgcccg tgcatgtggt ggtggcgcag accgacacac cgggcgaagc
     1081 catcgtggag ttgctgccgt tggagcagca ggccaagcag gaccgcgaag agcgcctgat
     1141 cgaccaggcg caggccaaca aggagctgat ccgcaacctg gcgcatgaga tcaagaaccc
     1201 gctgggcggg attcgcggcg cggcgcaact gctgcagatg gagatcgagt ccagggaact
     1261 caccgaatac acccaggtca tcatccacga ggccgaccgc ctgcagtccc tggtggaccg
     1321 gttgctggca ccgcaccgcc ggccgcacct ggtgggcgac gtcaatatcc acgaggtgtg
     1381 cgaacgggtc cgctcgctga tcctggcgga gtttccgcgg ggtttgcgcg tcatgcgcga
     1441 ctacgacatc tcgatccccg actttcgcgg tgaccgcgag caactgatcc aggcggtgct
     1501 caacattgcg cacaacgccg cacaggcgct cgccgagcgt gttgccgcag gcgatgccca
     1561 gatcactttc aggaccagga ttgcccgaca agtaactttt ggcaagcagc gctatcgctt
     1621 ggcactggaa ttgcatgtta tcgacaatgg accgggtgtt ccggactcca tcaaggaccg
     1681 tatcttctat cccctgatct cggggcgcga aggcggttcc ggactggggc tgacattggc
     1741 gcaaaccttt gttcagcaac accacggctt gattgagtgc gagagcgtcc ccggccgcac
     1801 ggatttcaag ttgctgatac ct
//
LOCUS       DAIDFG010000425         1800 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_425_length_1800_cov_17.406304, whole genome shotgun sequence.
ACCESSION   DAIDFG010000425 DAIDFG010000000
VERSION     DAIDFG010000425.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_425_length_1800_cov_17.406304"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          316 a          599 c          555 g          330 t
ORIGIN      
        1 ctcactgcgt gtagctccct gccccccgag ggggccgctg cgcctgcggg ccggcgaagc
       61 cggacccgcg gctcttgctg gtctgaagag tccacacggc tgcccgggca atccgttcat
      121 tccgggtttc tcggtcaaat cggccgtcag cccttgattg acggtcgtca gtagctactt
      181 tattgatagc aaaagtaaaa accgctcgac aatggcctgc gcgtaatggc tggccacctg
      241 ctcgacaaag ctcgagaaat caacatgcgc gctgtcatcg gcccggtctg aaatcgtgcg
      301 catggcggca aagggcacgc cgtaatccag gcagacttgg gccacggcgg cgccctccat
      361 ctcgacggcc agcaccgcgt gtccggcgcc ggccagggcg gcctgcaggg cctgcgactc
      421 ggcagaaccc gagacaaacc ggtcgccgct ggccaacagg ccctgatgca cgcgggcctg
      481 cgggaagcgc tgcacagacc tgcccgcttt caccggggcc agcgcggcgc aggccgccgc
      541 gtagagcagc gcggtcaggg ccgtgtcacc gtcgaaccgg gctttcccgt aaaacgggac
      601 ctcgtagcgc ggaaaaagcg gcgagacatc gagatcatgc tgcaggaaat cggtcgccac
      661 caccacatct ccgacctgca caccctcgcc cagcccgcca gccacgccgg tgaacaccac
      721 acggccgaca ccgaaacgct cgatcagcgc cgtcgccgtg gtggcagcgg ccaccttgcc
      781 aatttttgac agcgccagca ccacgggctg accatgcagc tcaccctgcc agaagtcgcg
      841 gccggcatga cgggttttct gcggtcgctg caacagttcc agcaggccgt gctgttcttc
      901 ggcgagggcg ctcaggatgg cggtagggtt catggccgca agtttgcccg aactggtgcc
      961 cactcctggc cgcaccagga aaaaaaggca gccggagccg ccttttttct ttcacctgcc
     1021 gggcctgagc cgcctcctac ttcttgtcgc gcaactcccg gcgcaagatc ttgccgaccg
     1081 gggttttcgg cagctcggta cggaactcga ccaccttggg ctgcttgtag ccggtcaggt
     1141 tggtcttgca gaactcgcgc acctgggctt cggtcaggtc cggatttttc ttgacgatga
     1201 ccagcttgac ggcttcaccc gatttgtcgt cgacaacgcc gacgcaggcg cattccatca
     1261 cgccttccat gccggaaacc acgtcctcga cctcgttcgg gtagacattg aagccgctga
     1321 cgagaatcat gtctttcttg cggtccacga tcctgaagaa accgcgctcg tcgacgacgc
     1381 cgatgtcgcc ggacttgaag tagccgtcgg gcgtcatgac cttggcggtt tcgtcgggcc
     1441 gctgccagta gccggccatc acctgcgggc ccttgatcgc gatttcaccg ggctggcccg
     1501 ccggcacgtc gttgccgtcg tcgtcgagca gcttgaagta cgtgccggga atcggcacgc
     1561 cgatggtgcc ggtgaaggcc ttgctgttgg tcgggttgca actggccgac ggcgaggttt
     1621 ccgacaggcc gtaaccttcg cagatcgggc agccggtttt ttccagccag agcttggcaa
     1681 ctgcactttg caccgccatg ccgccgccca gcgaaacctt caggtgcgac cagtccacgg
     1741 tgttgaagtc ggggtggttg gccaagccgt tgaacagggt attgaccgcc gggaagctgt
//
LOCUS       DAIDFG010000426         1800 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_426_length_1800_cov_13.868195, whole genome shotgun sequence.
ACCESSION   DAIDFG010000426 DAIDFG010000000
VERSION     DAIDFG010000426.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_426_length_1800_cov_13.868195"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          409 a          556 c          504 g          331 t
ORIGIN      
        1 ccgtacgcca tcgacgcgct ggctccccac tacagccagg agacgtttga gtaccaccat
       61 ggcaagcacc acaacgccta tgtggtcaac ctcaacaacc tgcaaaaagg caccgagttc
      121 gaggccatga cgctcgaaga gatcatcaag aaatccagcg gtggcgtgta caacaacgcg
      181 gcccagatct ggaaccacac ctttttctgg gactgcatga aaccggccgg cggcggcgag
      241 ccaagcggtg cgctcgccgc ggccatcaac gccaagttcg gctcctacgc ggctttcaag
      301 gaagcctttg tgaagtcggc tgtcggcaac ttcggttcgg gctggacctg gctggtgaaa
      361 aaagccgatg gctcggtgga catcgtcaac accggcccgg ccggcacacc gctgaccacc
      421 gccgacaagg cgctgctgac ggtggacgtg tgggaacacg cctattacat cgactaccgc
      481 aaccagcgtc ccaagtttgt cgagactttc ctcgacaagc tggtgaactg ggacttcgcc
      541 gccaagaact tcgcctgaca tccgttcggc gggttgccgt gttcctcgga gagcgggctt
      601 tgcgtaaaac aacctggtgt taaatctcgc caagctgttt aaaccatcct tctcgaacaa
      661 aagggctcga agatcaaatc ttcgagccct tttgttttat ggccgtcaca gggcttgcgc
      721 tcccggggct ggcggcaccc ccttgaccaa gcctccgcgt tgggctgcgg acggtctttt
      781 catctgccgg caccggtatt ttttgtctgc gtggaaaaaa agtgggttca aatgcctgca
      841 atttcgtatc atgatattgc attgcataga atgcagcttg ggtcagattc ccctaaggaa
      901 agtcagacag catccgatct tgacctccgg tctgtgacgc agccggtggc gttccccata
      961 acaatttctg gagataagcg atgagcaccg agcaacccac catcatctac accctgaccg
     1021 acgaggctcc gctactggcg accagcgcct tcctgcccat cgttcgcacc ttcaccgcgc
     1081 ctgcaggcat caaggtcgag accgccgaca tttcggtggc cggccggatc ctggggcagt
     1141 ttccggaatg cctgactgcg gaacaacggg tggccgatac cctgacagag ctgggcaagc
     1201 tgacgctcaa gccgcaggcc aacatcatca agctgcctaa catcagcgca tcggtggccc
     1261 agctcaagga cgcgatcaag gaactgcagg gcaagggcta caagctcccg gactacccgg
     1321 aaagtcccaa gaccgacgag gacagggaca tccgcgcgcg ctacaacaag tgcaccggca
     1381 gcgcggtgaa cccggtgctg cgcgaaggca attcggaccg ccgcgcaccc agggccgtga
     1441 aggaatacgc gcgtaggaac ccgcacagca tggccgactg gagccaggcc tcgcgttcgc
     1501 acgtctcgca catgcacgcc ggtgacttct accatggcga aaagtccatc acgctggacc
     1561 gtgcgcgcga cgtcaagatg gagctgatca ccaaaagcgg caagaccatc gtactcaagc
     1621 ccaaggtcgc attgctggat cgcgagatca tcgactccat gttcatgagc aagaaggaac
     1681 tggtggcttt ctatgaaaag gaaatcgacg acgccttcaa gaccggcgtg atgttctcgc
     1741 tgcacgtcaa ggcgaccatg atgaaggtgt cgcaccccat cgttttcggc cactgcgtga
//
LOCUS       DAIDFG010000427         1709 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_427_length_1709_cov_19.915357, whole genome shotgun sequence.
ACCESSION   DAIDFG010000427 DAIDFG010000000
VERSION     DAIDFG010000427.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_427_length_1709_cov_19.915357"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          307 a          459 c          560 g          383 t
ORIGIN      
        1 tgctcggcgg caccggtaat cttgttctgg cggcccagtg ccaggatctg cgtgaagccg
       61 acatagaaga acaggccttc catcaggcag gcaaaaacga tcagcgactt cagcagggtc
      121 tggtccgctt cctgggtgcc ggtcttgaag ttggggtccg agatggcgtc gatgaaagga
      181 atcaggaact ggtccttgtc ccggatcgac tgcacttcgt tgtaggcgtt gaagatctcg
      241 ctttcgtcca ggcccagcga ttcggtgatg tactggtagg cgtgggtgtg aatggcttct
      301 tcaaacgcct ggcgcagcag aaactggcgg cactcggggg ccgtgatgtg ccggtaagtg
      361 cccagcacga tgttgttggc ggccagcgag tcggcggtga cgaagaaacc gaggttgcgc
      421 ttgaccaggc ggcgctcgtc ttccgtcagg ccgttcgggt ctttccagag cgcgatgtcg
      481 cgcgtcatgt tcacttcctg cggcatccag tggttggcgc agctggccag gtatttttcc
      541 caggcccatt tgtacttgaa cggaaccagc tggttcacgt cggtctggcc gttgatgatg
      601 cgtttgtcgg cggctttgac gcgatgggcg gtgggtgcca cggtggccgg tgctgcacgc
      661 ccgcccgagg aagtcagggc gctgtcgtcg agcatgcggg ctgcagcatg gggctgcaaa
      721 gaaagaggcg gaagttccat cgagcggcct tcaggaaggg cgctggcaag aggtttttgc
      781 aacgatggtt tagcttcttc gtcccaagtc aacatagatg tttccaataa gttggattat
      841 gaaagcagcg atacaaaatg aaaagtgttc attcacatcg ctctgaatta gtgttgctca
      901 attacatcga atcaggcacc ggatttttcc agcgccgtga tccgtttttt attggcaggc
      961 ctcacatcct ggatcgtcga tcgcgcaaaa cttgatgtcc gtcgcaggtg acgcattcat
     1021 ctgcgcctgc gctgtggctg ctgccgcatc gagtgcgttc atgctaccgg tgtcatcggt
     1081 gttgccggaa gcgaccgcat tcatcttgcc ggctttcacg gtggactttt ctgcatgcgt
     1141 cgcgctcatg gtgcgcaggt agtaggtggt cttcaggccg cgcagccatg cgagcttgta
     1201 ggtgtcgtcg agcttcttgc ccgaggcgcc ggccatgtag atgttgagcg actgcgcctg
     1261 gtcaatccat ttctggcggc gtgacgcggc ctcgaccagc caggtggcgt cgacctcgaa
     1321 cgccgtggcg tacagggcct tgatgtcctg cggcacgcgg tcgatggggc gcagcgagcc
     1381 atcaaagtgt ttgaggtcca ccaccatcac gtcgtcccac aaacccaggc gcttgaggtc
     1441 gcgcaccagg tagtggttga tgaccgtgaa ttcgccggac aggttggact tgaccgagag
     1501 gttgccgaag cagggctcga tgcaggcgtc cacgccgatg atgttggaga tggtggcggt
     1561 cggtgcgatg gccacgcagt tggagttgcg catgccgtgc tcggcgatac gcgcacgcag
     1621 gacgctccag tcgatggcgc tgctgcgatc gacttcgacg taaccgcctc gcgcttcggc
     1681 cagcagatca agcgagtcga tcggcagga
//
LOCUS       DAIDFG010000428         1697 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_428_length_1697_cov_13.348356, whole genome shotgun sequence.
ACCESSION   DAIDFG010000428 DAIDFG010000000
VERSION     DAIDFG010000428.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_428_length_1697_cov_13.348356"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          356 a          486 c          473 g          382 t
ORIGIN      
        1 actggaccgc atcgtcaagg tgcctggcct gtccaatatc tgggtgccac cgattcgcaa
       61 ccgcatcgac atgctggcga ccggcatcaa gagcccggta ggtgtcaagg tggcggggac
      121 cgatctgatc acaatagacc gcctgactgg cgagatcgag cgggcgctca aagacgttcc
      181 cggcgtcacg agcgccttgg ccgagcggct gaccggcggt cgctacatcg atgtcaatat
      241 caagcgcgat gcggcatcac gctttggcct taacattgcc gacgtgcaat cggtcatcag
      301 cacagccgta ggcggcgaga acattggtga gaccgtcgag ggtctgcagc gcttcccgat
      361 caatgtgcgc tatccccgtg aaatccggga ttcactggag aagctgcaca gcctgccctt
      421 tgtaaccgag cgcgggcagc gcctggtcct gtccgacgtg gcggagatcc gtatcaccga
      481 cggtccaccc atgctgcgca gcgaaaacgc ccgcttgtca ggttgggtct atgtggacat
      541 ccggggtcgc gacctgcgct cggccgtgca agacatgcaa aaagccgtca ccgacaaggt
      601 caagctaccg ccaggctatt ccgtctcctg gtcggggcag tttgaattcc ttgagcgcgc
      661 gacggccaag ctcaaagtgg tggtgccgtt caccctgctc atcatcttcg tgctgctgta
      721 tctcactttc aaacggttcg atgaagcgtt cctgatcatg gccaccctac cgttcgctct
      781 cgtagggggc atttggctgc tgtacatcct gagctacaac ctgtcggtgg ccggtgcggt
      841 agggtttatc gccttggccg gtgtatctgc ggagttcggg gtgatcatgc tgctttacct
      901 caaatcggcc tgggatgacc gacttgcgag aggcaagacc actacggccg atctgctgga
      961 cgcgattcag gaaggtgctg tattgcgtgt gcggcccaag gcgatgacgg ttgctgtgat
     1021 cttggctggc ttgtttccca tcatgtgggg tacaggcaca ggttccgagg tcatgcagcg
     1081 cattgcggca cccatggtcg gtgggatgat caccgccccc ttactgtcca tgttcgttat
     1141 tccagccgca tatctgctga tgcggcgccc ttcgtccggc gaaatgaagt tgacaacatt
     1201 ttggaaatga aagtgtcaac actcaatcac tttccttaat ccatgcgggt tccgggtcac
     1261 tattgcaatc cgtcgatttc aaaaaatccg cagctcggtg gcttttttcg gccttctcta
     1321 tgaaatgaag tgccaacagg ctgttgaatt gcgttgaagt ctgacccgag gggatgcgga
     1381 caaaaacttg gactaccagg aggtagttca tgtattcata cgaagacagg attcgagcgg
     1441 tcgagctctg caccccgcct tcgcttacgt gctgatcaat caagtaccaa ctgtactaac
     1501 gctgaatttt ctctcctcaa taagaccgtc acccagcgta aagtagaaac acgcgccttc
     1561 acccaatgcc gcctccgccc agattcggcc accatgccgt tcaactatcc gtttcacaat
     1621 cgacagaccg ataccaagtc caacaaattc atcggcacga tggaggcgct gaaagggctc
     1681 gaacaaatga tttgcgt
//
LOCUS       DAIDFG010000429         1672 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_429_length_1672_cov_15.286951, whole genome shotgun sequence.
ACCESSION   DAIDFG010000429 DAIDFG010000000
VERSION     DAIDFG010000429.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_429_length_1672_cov_15.286951"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          263 a          515 c          598 g          296 t
ORIGIN      
        1 cttcggccgt ctccgtgaag cagtggaaca ccccgccggc ccgccctgct ccgtctgctg
       61 tctcgccctc ttccttcagg atggccagcg tgtcgtttga tgcgctgcgg gtgtggatca
      121 ccagcggttt ttgtgtctgg cgcgcggcgc ggatgtgggt gcgaaagcgt tcgcgctgcc
      181 agtgcatgtc ggcgatgctg cgctcgccca ggcggtaata gtccaggccg gtttcaccga
      241 tggccaccac cctgggcaag gcggcgcgtt gcaccaggtc ggccaggctg ggttcggtca
      301 caccttcgtt gtcggggtga acgccgacgc tgcaccagaa gttgtcgtag ctggtggcca
      361 gcgtgtgaac ggtctcgaac tcctcaagcg tggtacagat gcacagcgcg cgatccacca
      421 gggcttgcgc catggcttcg cgtatttgcg gcaactgggc ctgaagctca ggaaaactca
      481 ggtggcaatg ggaatcggtg aacatcggca aggctttatt gggtatcaaa aatggctgta
      541 gcccatacca gacgggcgca ggcagctatc aaaaaggaag cagcccgagg ccgggcctgc
      601 aacggcctag atcgtctgcg tcggacggtc ggaggccagg tgggcgccga ggatttcctc
      661 gatcttgatc ttcagcaggc gggacttttc gtcgtcggga aaacgcacgc ccacaccctg
      721 ggtccggttg ccggaggccc gcgccggggt aacccaggcc accttgcccg ccaccgggta
      781 gcgttgcgtg tcttcgggca aggtcatcag cacataaacg tcgtcgccca gcttgtagtc
      841 gcgcgccgag gggatgaaaa cgccgccttc ggtgaacagc gggatgtagg cggcgtagag
      901 cgcggctttt tcacggatcg ccagctggat gacgctgggc cggacggcgc tggaactggc
      961 ggcggtggca ccggcagcac cgggagaacc agcaggaggc gttgtactca taagcctagt
     1021 ttatcgggtt gaccgtcggg ctgtcagcaa gaaaccgcgc ttattttgaa ttgagtgcca
     1081 gctgcgcgcg gctgactagc gcttcgagca tcaggccggg gttgtagggg tgttccacgg
     1141 tgcgcgccgt ggcggccagc tccttggccc agacgcccag cgcggcaaag ccggggccgg
     1201 cggccgcgcc agttgcaccg ggtttgccgg cagcctgcac cggcaagtcg gcgtggcgaa
     1261 aaaaccgcgg ttcggcaccc gcccgggtgg ccagcatgtc gtggcagagc ttttgcagcg
     1321 catcgacggc ctgggccggc ccgaagtcgc tgagcgcccc gacctcgccc cgggccatgg
     1381 ctttgggcag ggcctgccag tgctgggcta tggtgctgct cggtccggcc tgggcgcgtt
     1441 ccagcgcgtc ggcgggacgg ccaccggcgg ccgccagcag ggtatgcagg tcggcagcgg
     1501 caggtgcttt gtcggccttg ccttccggcc cggcgcgctc ggccagccag gccagcgcct
     1561 ggtcaaaatc cggccagtgc atggtgtgac cctggcagcg gctgcggatg gtggggagca
     1621 actggtgcgc ggcctcggtg gcgaggatga actgcaccgc accgggcggc tc
//
LOCUS       DAIDFG010000430         1657 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_430_length_1657_cov_15.759051, whole genome shotgun sequence.
ACCESSION   DAIDFG010000430 DAIDFG010000000
VERSION     DAIDFG010000430.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_430_length_1657_cov_15.759051"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          314 a          507 c          499 g          337 t
ORIGIN      
        1 gatgccgccc ttgactggct ggccaatggt ttgctcgccg ccagctggtg gcaaattgtg
       61 ctgtacacac tggtgacgac ccacatcacc attgccagcg tgacgattta tctgcaccgc
      121 caccaggcgc accgtgcgat ggacctgcac cccatcccct cgcatttttt ccgtttctgg
      181 ctctggctgg gtaccggcca ggtcaccaag gagtgggtgt ccattcaccg caagcaccac
      241 gccaaatgcg aaacgtcgga agatccgcac agcccgcagg cctacggcat caagaaagtg
      301 ttctgggaag gcgccgagtt gtaccgcgct gaaagcaaga acatggaaac catggtcaag
      361 tacggccacg gcacgccgaa cgactggatc gagcgcaatc tctacacccg ctacagctgg
      421 cagggcgttg gcctgatgct ggtcatcaac ctggccttgt tcggcgcagc cggcctggcg
      481 gtctgggccg ttcagatgat ctggatcccc gtgacggctg ccggcatcat caacggcatt
      541 ggccactact ggggttaccg caacttcgag gcaccggatg ccagcaccaa tgtgtcgcct
      601 tggggcgtca tcattggcgg cgaagaactg cacaacaacc accacaccta tcccacctcg
      661 gccaagttct ctgtcaagcc ctacgaattc gacattggct gggtgtacat ccgcgccatg
      721 gaaaaagtcg gtctggcgac ggtcaagaag gttccgcccc ggcttcagct cggggacatc
      781 cagcctgtgg ccgacgagaa aaccctcgaa gccctgattg cccaccgcta cgaggtgatg
      841 gccggttttg cgcgtgaact gcgccgcgcc ggcaaggctg aaatcgaggt gctcaaggcc
      901 cgcaaggctg atatctcggt actgcaggcg gccaaccgct ggttgcatcg cgacgatgac
      961 aaggttcctg ctgcggccaa gcctcaggtg gcgcaggccc gtgccgagca cccggtgctc
     1021 gacaagatgg tcaccatgcg cgaagagttg cgccagatgt ggctgtcgag gtcggcgtcg
     1081 cgcgagcagc tggccacgga tctacaggcc tggtgccgcc gcgctgaaga aagcggcatc
     1141 gcggccctgc aggagttttc gatgaagttg cgcgccgcac gcgcctgatt ctgccgtcag
     1201 gggcttgccc cctgtgctgc aaaccgcctc ggtgcctcgc gcccaggcgg tttttctttg
     1261 gggtctcggt ttcgcccagc cgacaaggga cggggcggcg acagggatta cttcggaagt
     1321 accagccgct ggccgctgga aagccccgtg acgccacaca cagactttgc gaatgctgct
     1381 gtctgctgct gtctgctgct gtcttccccg ggccatggtt acaccaggga aaaccggccg
     1441 gccatgaaaa acccgccggg acactgggtc cgggcgggtt ttttcatggt acagcacaga
     1501 gtgctgcagg ttcaggctga attacttcag cttgatttcc ttgtattcca catgcttgcg
     1561 tgctttggga tcaaatttca tgatcagcat tttgtcaggc gtggttttct tgtttttggt
     1621 ggtcgtgtag aaatgacccg tgccggcagt cgattcc
//
LOCUS       DAIDFG010000431         1627 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_431_length_1627_cov_16.737913, whole genome shotgun sequence.
ACCESSION   DAIDFG010000431 DAIDFG010000000
VERSION     DAIDFG010000431.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_431_length_1627_cov_16.737913"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          345 a          539 c          475 g          268 t
ORIGIN      
        1 catgctgtat gacctcaagc agatcctcga tggcggtgag accaactaca tcagcgctac
       61 cttggccctg tacctggacc tgttcaacgt gttccaggcc ctgctggccc tgctgggcat
      121 cgcgggtggc gagcgcgact gagcttgctc gcattcaaaa aaagggcctt cgggcccttt
      181 tttcatgggc tgtcgcgccg gggcagctcc gtgcacgcct gcggcctccc ggcaaaaagc
      241 tatctattca atagcaaact tcccttatat ttaaaggatt agattcatat ttgactgaaa
      301 actcgcggcc agacgaactt gcgctggatc aacagagttc gcgttaaccc ttataaaggt
      361 gcacaacaaa tacatcttct aattacactg tctgcaggca cccggacaac agagatccgg
      421 atgccttcaa agaagaaaga aggttgtttg tatgcgtacc cctcattcaa ggaatttttc
      481 actgctggcc ctcgctgccg ccatggcgct tgccagtctg ggcgcggctg cggcgccgcc
      541 cgcagccacg cagagcggcg catcgcgcgc cccggccact gaggaggcca agggcgccgt
      601 caagaagtac acgctcgaaa ccggcatcgt caacggcaag atggtgttca tcgacgaccg
      661 cggccaggcc aacccggtgc tgcacgccaa tgtgggcgac acggtggaga tccgcctgaa
      721 aagcggcgaa ggtgcacagc acgacatcgc catccccgag ctgaaagtgc agtcgcagaa
      781 attcgacgcc gccagcggcc ccacgaccgt gcgtttcacc gtcacgcagg cgggcaagtt
      841 cacctactac tgcaccattg caggccatcg ccagatcggc atggaaggcg tgtttgaagt
      901 ggccgggacc agcattgcga gcgcagcgcc caaagcagcg gcgaagacaa ccggcgttcc
      961 cggtgccgag ctgtacaccg cgcccgccat gcggcccgcc gacgcggccg ccgtgagtgt
     1021 ggccatggac ccggctgccg tgcctgcccc gatcggcgcc cgcgcgcccc agcacctgaa
     1081 gtaccgcatg gaaaccgtcg agctgaccgg caaactcgac gacggcacca gcttcactta
     1141 ctggaccttc gaccgcaagg tgcccggccc catgctgcgc gcccgcatcg gcgacaccat
     1201 cgagctgacc ctggtcaatg cacgcgacag caagatgatc cattccatcg acctgcacgc
     1261 agtgaccggc ggtcacggtg gcggcgaaca cacccaggtc ggccccggcc aggagaaaac
     1321 catcaccttc aaggcgctga acgccgggct gtatgtgtac cactgcgcca caccgctggt
     1381 gccgcagcac atctcggccg gcatgtacgg catgatcctg gtcgagcctg aaggcggcct
     1441 ggccaaggtg gaccgcgagt actacgtgat gcagggcgac atgtacacca cccgcccgca
     1501 cggcgccaag acgcaccagg agttcgacct ggacaagatg gcggccgagt tgcctgacta
     1561 ctacgtcttc aacggcgcgg tgggctcgct gaccagggag cacaaactca ccgcccatgt
     1621 cggcgag
//
LOCUS       DAIDFG010000432         1585 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_432_length_1585_cov_15.311765, whole genome shotgun sequence.
ACCESSION   DAIDFG010000432 DAIDFG010000000
VERSION     DAIDFG010000432.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_432_length_1585_cov_15.311765"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          323 a          550 c          522 g          190 t
ORIGIN      
        1 ggcggcccgg cgcggcaccg gcagcagcag cacggccagc acgccgccca tgatcaggcc
       61 gacacgcacc ggcaggtcca gccaggccca ggcgtggtcc gggccgtaac tgagcgcggc
      121 ggacagggcg gtcagcgcga tgccggccgc cagcgtgagc atcaggaaaa cagcgcgttg
      181 cagcaaggtg cggatcacgc agtagccgag caggcaggga atcagcgcgc ccagcgccac
      241 acagatcact tcgacgccgg ggaccagcgg ctgcagctct atgtcgcgca ccggcagcca
      301 ctcgagaaac ggcgtatcgg ccagcacgtc ggcaagcgcg tactccagcc gttccagcac
      361 ctgccccaga cccagcggca ccgaggccgg gaacagcagc gcgaccggcc acagggccag
      421 cagcaccagc gcaccccggg cgtcgcgcac aaaccagcgc gcccgaaagc ggctccagcg
      481 gtccatcgca ccggcccgct ccagcagcca ggcgctgcag gcgcccagcc aggccccggc
      541 cgtgttcagg acaaagtcga cattggaggg aatgcgcgca ggcaggtaac tttgcagcga
      601 ctccatcacc agcgacaggg tgcccgcgcc gagcacggcc acagtgaccg cccagcgcgt
      661 gcggccgctg cgcagcgccg ccagggccag cagaaaaccc agcggcgcgt aaccggccag
      721 gttgatgccc acatcaaaac cggtccagta gcgcggcagg ggcgccgtca gaaaggtcag
      781 cggcgagatg ccctggttgc gccactcggc aaacgggtag aggctggcat aaacaatcag
      841 gcagaccgtg gccagtgcca gcggccagga ggcagttggc tcacccctat cgtccgcact
      901 ccgcttcgct gcgtgtcgtt ccttatcccc ttccagggaa agaggtggca cacccctgac
      961 atcctcacga tgctgcgcat cgtgtagttc tgccccccct tgcagggggc gacgcatggc
     1021 gcccggcaaa gccggttcgc tcgcgtctgc tggtgaaggc ggggatggca cgctggcggc
     1081 cttgtgatgc tagaacggct tgaccacaac caggatcacc gcagcgagca gcagcagcac
     1141 cggcacttcg ttgaaccagc gccaccacac gtggctgcgg gtgttgcgcc gggccacaaa
     1201 ggtcgacaac atgcggctgc aggcatggtg gtagccgagg gccagggcca cgatgacgag
     1261 cttggcgtgc atccagccat tgccggggcc gcgccctatg ccgtaaccca gccacagcca
     1321 caggccgaaa gccagcgcgg gcacggccag gatcgtggtg aagcgcagaa gcttgcgtgc
     1381 catcaggatc aggcgctcat gctcggccag actttccggc ggcaccatcg ccaggttgac
     1441 aaaaatacgc ggcaggtaaa acaggcccgc aaaccagctg gcgacaaaaa cgatgtggag
     1501 tgatttgacc cagagcatgc acaaagtgta gcccccacgg ttgctcactg cgtgtagcgc
     1561 cctgcccccc gagggggccg ctgcg
//
LOCUS       DAIDFG010000433         1560 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_433_length_1560_cov_15.099003, whole genome shotgun sequence.
ACCESSION   DAIDFG010000433 DAIDFG010000000
VERSION     DAIDFG010000433.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_433_length_1560_cov_15.099003"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          264 a          537 c          500 g          259 t
ORIGIN      
        1 agccttcttc accggcaccg ccgcctaagt cacgcccatc cgcgaactcg accgcatcga
       61 aatcggcgcg ggctcgcgtg gccccatcac cgaaaaaatc cagagcgcgt tttttgacat
      121 cgtcaacggc cgcaacccca aatacgccgg ctggctggcc aaggtctgat catgagtacc
      181 tctttcaccg tcgaactgct cgccaaggac ctgaacccgc aaggcggcgt gttctgtccc
      241 agccccaagg ccgacatggc cgtgtggaac agccacccca aggtctacct cgatgtggcg
      301 cgcaccggcg acgcgaagtg cccttattgc ggcacgactt acgcgctgaa agcaggcgag
      361 cacgtcggcg ctggccactg agatggccgc gcctggccgt tcgggctgag cctgtcgaag
      421 ccttcttcag acattgcggt ttgcaaacac ccttcgacaa gctcagggcg aacggaagcc
      481 atgtcagata actcgcaccg ttccctcgtc attgctcccc agtggatagg cgacgcagtc
      541 atgaccgagc cgctgctgcg gcgtctgcat gcacgcggcg agcaactcac ggtcggcgcg
      601 ctgccctggg tcgcaccggt gtaccgtgcg atgccgcagg tgcacgaggt catcgagttt
      661 ccgtttgcgc atggcggcct gcaactgaag gcgcggcgcg cgctggccaa gcggatcgcc
      721 ggccagttcg acaccgccta cgtgctcccc aactcgctga aaagcgcctt gctgcccctg
      781 ctggccggca ttcccaaacg tgtcggctac ctgggtgaag cgcgcatcgg cctgctcacc
      841 catcgcctga aaaatccggc caaaggccag cgaccgccca tggtggcgtt ttattcggcg
      901 ctcagcggcg acacggatct ggacacggac cggccgcagt tgcagatgcc ggcggccgag
      961 gtgacgcagg cgctggccga gctcggcctg gagcgcggcg cttatgacgt ctttgcaccg
     1021 ggtgcggaat acggtccggc caagcgctgg cctgcggcgc atttttccga actggcgcaa
     1081 cgcctggccc tgccggtgct gttgctgggc tcgggcaagg aagcggcgct gtgcgaagaa
     1141 atcgccgggc cggccaacgc ggtgcgaccc ggtgcctgta tcaatctggc cggcaagacc
     1201 acgctggtgc aggcgctgtg cgccatcgca gcggcccgcc aggtggtcag caacgactcg
     1261 ggcctgatgc atgtggcggc ggcctttggt gtgccgcagg tcgccatctt cggctcgtcg
     1321 agcccgctgc acacgccgcc gctgagcgcg aaagccacgg tgctctggct gaaaaacgat
     1381 gcggcctacc tgccaccgct cgattgcgcg ccatgcttcg agcgcgaatg cccgctgggc
     1441 cacacccgct gcctgaacga catcagcgcg gcgcgggtgc tcgcggcttt gtcgccctga
     1501 cgctgaggga agcgctgaac aagtcccctg cggcgcgcga tcagccgggc tcgggcggcc
//
LOCUS       DAIDFG010000434         1519 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_434_length_1519_cov_14.668033, whole genome shotgun sequence.
ACCESSION   DAIDFG010000434 DAIDFG010000000
VERSION     DAIDFG010000434.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_434_length_1519_cov_14.668033"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          276 a          503 c          477 g          263 t
ORIGIN      
        1 gccggggcga gacccggctt gcaggcaagc ttcagccctt cgaaaaaatg attggtgagc
       61 aaacatggat cccggatcaa gtccgggatg acagatgtgg ggagaggttg ctatgaattc
      121 aatagctgct tgcgcccgcc acataatggc tggaaaccaa aaaactatga agaacgcact
      181 gccaccaccg ccgcaggctg ctctgcctgc aagcgctggt acttctgcca gagcgtttcc
      241 cggtcttcga tgaaagccgg gttgaccgga atacacgcca ccggacagac ctgcacacat
      301 tgcggctcat cgaaatggcc gacacactcg gtgcagcgat tcgggtcgat ctcgtagatt
      361 tccttgccca ggtaaatcgc ctggttcggg cattcgggtt cgcacacgtc gcagttgatg
      421 cactcgtcgg tgatcagcaa ggccatggtc agccctcctt ctgcgcctgc tgcatggcga
      481 tggcgacatt ggcgctgacc atctggccca catcgccgcc gagcttgctg atctcgcgca
      541 ccagggtgct gctgatgcat tgcagcggcg cgtccggcag caaaaacacg gtttccacct
      601 ggggccgcag cttgcggttc atcgcggcca tttgcgcctc gtagtcaaag tcggtgaggt
      661 tgcgcacgcc gcgcaccacc gcgacggcct gctcagccgc gcaaaaatcc atgatcaggc
      721 cgtcgaaagg cagcacgctg acgttggcca tgccttgggt ttcctcgcgc acctgctgca
      781 ggcgcgcgtc caggctgaac agcgttttct tgtggtgcgc cacggccacc ccgatgatga
      841 gccggtcaaa cagcccggcc gcgcggcgca cgatgtcctg gtgcccgagc gtgaagggat
      901 cgaaggtgcc ggaataaacc gccacacggg aactcggggt gcttgtcgcc atgcggcaat
      961 tatgcccgcc tgaccgtggt gccgcgagtc cctgccgctt gcagggctac gcgccttcca
     1021 gcgaaacgct cttgcgcacc agcaagtgaa agtgcaccgc gcccgctttg ccgctgcggt
     1081 gcagctgcag gccgtagtcg gccagtgcct cgtcggtcca ggcctgcggc gcctcgaggt
     1141 agacataacc gggctcggcc accacctgcg ccgccgcctt gagcgcgggc tcgaacagcg
     1201 gcgaatcgaa cggcgggtcc aggaacacca gttgcatgct ggccggtgtt gccagccgga
     1261 ggacggcgac gccatcgccg cgctccaccc gcaccatgtc ggcgtgcagc ctggcctgca
     1321 gtgccttgag ctggtccacc aggccggcat cctgctcgca cagcagcacc tcggcagcgc
     1381 cgcgcgaggc ggcctcaaag cccagcacgc cggtgccggc aaacacgtcg atgcagcgcc
     1441 atccgctcag gtcctggccc agccagttga acagggtttc gcgcacgcgg tcaggcgtcg
     1501 ggcgcaggcc gggcttgtt
//
LOCUS       DAIDFG010000435         1476 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_435_length_1476_cov_16.251935, whole genome shotgun sequence.
ACCESSION   DAIDFG010000435 DAIDFG010000000
VERSION     DAIDFG010000435.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_435_length_1476_cov_16.251935"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          232 a          450 c          485 g          309 t
ORIGIN      
        1 gcggccaaat gaagtccccc tccatcgccc agcggggcga gggaggggtt aggggcggga
       61 gtggggagtt gacactactg cccgaagtga accggggacg gagcgtgatg cctcaatgga
      121 cttatcccac cttgcccagc agcaaaaact ccatcaaggc cttctgcacg tgcagccggt
      181 tttcggcttc atcccagaca acggactgcg gcccgtcgat cacctcggct tcgacctctt
      241 ccccgcggtg cgccggcagg caatgcatga agagggcgtc gggtttggcg gccttcatca
      301 tgtcgctgtc cacgcaccag tcggcaaacg cctttttgcg ctcttcgttc tcggcttcgt
      361 aacccatgct ggtccagacg tcggtggtga ccaggtcggc gccggcgcag gcctgcatcg
      421 ggtccttgaa gaccttgtag ctgtcggccg agcggatgcc ggccaccgac tggtccacct
      481 cgtaaccggt cggtgtgctc aggtgcacct tgaagccgag gatctcgctg gcctgcagcc
      541 aggtgttggc catgttgttg ccgtcaccga cccaggcgac tgtcttgccc ttgatgctgc
      601 cgcggtgctc gatgtaagtg aagatgtctg ccaggatctg gcaggggtgg aactcgttgg
      661 tcaggccgtt gatcaccggt acgcgcgagt gttcggcaaa cagctcgatc ttggtctgtt
      721 cgtaggtgcg gatcatcacc aggtccacca tgcggctgat gaccttggcg ctatcctcga
      781 tcggctcggc gcggcccagc tggctgtcgc cggtggtcag gtgcaccacg ctgccgccca
      841 gctggtacat gccggcttca aaactcacgc gggtgcgggt cgaggctttt tcgaaaatca
      901 tggccagcgt gcggtccacc agcggctggt gtttgtcgta ggccttgaat tttttcttga
      961 tcagggcggc gcgctcgaag acgtaggcat attcggcggc attgaagtcg ctgaactgca
     1021 ggtagtggcg gattgcggtg ttgaccgggg tgctcatggt ttttccgcca gcaactgctt
     1081 gatcagcggg cacagaatgg ccacgacttc gtcagcctcg gcttgcgtga tgatcagcgg
     1141 cggcaccagc cggatcacac tgtcggccgt cacactgatc aggaggccct tgtcggcagc
     1201 gcgtttgacc aggtcgccgc agggtttggc caggtcgatg cccagcatca ggcccatgcc
     1261 gcggacttcc ttgaccgtgc ccgaagccag ctcggcggcc agttcgcggc caagggccaa
     1321 cttcaggtgt gcgcccacct tggcggcgtt ggccagcagg ccttcttctt ccatgatccg
     1381 gatggtctcc accccggcgc gcatggccag cgggttgccg ccgaaggtgg tgccgtggtt
     1441 gccgggggca aagatgtggg ccgctttcgg gcctgc
//
LOCUS       DAIDFG010000436         1470 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_436_length_1470_cov_16.124382, whole genome shotgun sequence.
ACCESSION   DAIDFG010000436 DAIDFG010000000
VERSION     DAIDFG010000436.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_436_length_1470_cov_16.124382"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          233 a          478 c          444 g          315 t
ORIGIN      
        1 acgccttcgt aggtaaccat ggagccgtcc ttcctgagct tggcgccgat gaccttgcca
       61 tgctcgtcgt gcacgtcgtc cacatcggcc ggccagaagt attcgacgcc gcccttgtcg
      121 cccttgcggg agtagatatg gttgagcacc atgccctgcg tcagcagctt ggtgaaaggt
      181 tcgtccacct tgaccaggcc catgtcgcgc atgaccttgg tccagaaccg cgcatacagc
      241 aggtgcagga tggcgtgttc gatgccgcct atgtactgat ccatcggcat ccagtaggcc
      301 gcgccctccg cgaccatttt gtcctcgttc ttcgggtcgc agtaacgcat gaagtaccac
      361 gagctgtcca cgaaggtgtc catggtgtcg gtctcgcgcc gcgccggctt gccgcagacc
      421 gggcaggtca cgccggcgtg gaaaccaaca tgcttgtgca gcggattgcc cgagccgtcg
      481 ggcacgcagt cctgcggcag caccaccggc aggtcttttt ccggcaccgg caccgcgccg
      541 tgttcctcgc agtgaatgat gggaatcggc gtgccccagt aacgctgacg gctcacgccc
      601 cagtcgcgca ggcgccaagt ggtttttttc tcgcccaggc ctttggctgc caggtcggcc
      661 gccacggcat cgacagcggc cttgaaaacc aggccgttgt acttgccgga attgatcgcc
      721 acgccctgct gcttgtcgcc gtaccagtcg gcccaggcct caaggctgta ggttttgccg
      781 ggcacatcgg tgacctgctg gatcggcagc ttgtactttt tcgcgaactc gaagtcgcgc
      841 tcgtcgtgcg caggcacacc catcaccgcg ccgtcaccgt aactcatgag cacatagttg
      901 ccgacccaga cctcgacttt ttcgccggtc agcgggtgcg tgacaaacag gccggtagcc
      961 atgccctttt tttcctgcgt ggcgagttcg gcctcggtgg tgccgccgga cttgcagtcc
     1021 tcgatgaagg ccgcgagttt cgggttggcc gccgccgcgt gaatcgccag cgggtgttcg
     1081 ggtgccacgg cgcagaaggt cacacccatg atggtgtcgg cgcgcgtggt gaagacgtac
     1141 atcttcccgt cgccgatcaa ggcgccagaa gcgtccttga tgtcgtgcgt gaacgcaaaa
     1201 cgcacacctt cgcttttgcc tatccagttt tcctgcatca gcttgacgcg ctcgggccag
     1261 cccggcagct tgttgctgac gctgtcgagc agttcttcgg cgtagtcggt gatcttcagg
     1321 tagtagccgg ggatctcgcg tttttcaacc acggcgccgg tgcgccagcc cttgccgtca
     1381 atgacctgct cgttggccag cacggtctgg tccaccgggt cccagttgac aacctgggtc
     1441 ttgcggtagg cgacgccctt gtccagcatc
//
LOCUS       DAIDFG010000437         1439 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_437_length_1439_cov_19.176301, whole genome shotgun sequence.
ACCESSION   DAIDFG010000437 DAIDFG010000000
VERSION     DAIDFG010000437.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_437_length_1439_cov_19.176301"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          261 a          464 c          446 g          268 t
ORIGIN      
        1 gaggaactgc tccagccagt ccaccgactc ggcgtccagg tggttggtcg gctcgtcgag
       61 cagcagcatg tcgggcttgg acagcagcag gctgcacagc gccacgcggc gtttctcgcc
      121 acccgagagg acgccgacct tggcatccca gggcggcagg cgcaacgcgt cggcggcgat
      181 ctcgagctgg tgttcggagt cggtgcccga ggtagcgatg atggcttcca ggcgcgcctg
      241 ctcggcggcc agcgcgtcga agtcggcatc gggttcgccg taagcggtgt agacggcttc
      301 gagctgggcc ttggcggcaa acaccgcacc catgccggcc tccacgctct cgcgcaccgt
      361 gtgctcgggg tccagcttgg gctcctgcgg caggtaaccg atgttcaggc ccggcatggg
      421 catggcctcg ccttcgtact ctttgtccag accggccatg atcttgagca gcgtggactt
      481 gccggaaccg ttggtgccga gcacgccgat cttggcgccc gggaaaaagc tgagggaaac
      541 gtctttcaga atctgacgct tgggcggcac gatcttgccg accttgttca tggtaaatac
      601 gtactgggcc atcgggatta acttctctta aggggaaaac aacgcaggga acctgaccgg
      661 accggcctgc aaggcaggct ggccggcggt gatgctgcaa acaaaaacca cacgccttag
      721 ctggggggca ggcaccctga atcaaggcgc gtttctggcg tgaacccccc gattatcgtt
      781 ccatgcgaca atacacgggt tgctggctcc agttgcctgc agcaattagg cacaccttcg
      841 gtgacagttt gggtgcccag cctgactttt ttacccgtct gcctttcact ggcggaggct
      901 tcaacagccc ccgactggcc gatctttggc acctccgcgt gcaccatcaa tgacttttga
      961 aaatctgaac ttggccccgg ccattcttaa agccgtgctt gagcagggct ataccgttcc
     1021 caccccgatc caggcccagg ccattcctgc cgtgctggca ggcggcgacc tgctaggcgg
     1081 cgcccagacc ggcaccggca aaaccgcagc cttcgtgctg cccatgctgc agcgcctgtc
     1141 gtccgagccg tccctgaaaa acaagcgcgg cgtcaatgcg gtgcgctgcc tgatcatgac
     1201 gccgacgcgc gaactcgccg cccaggtgga agaaagcgtc cgcgtctacg gcaaatacct
     1261 tgaactctcc tccatggtga tgttcggcgg tgtcggcatg ggcgcgcaaa ttgaaaaact
     1321 gcgccgcggc gtcgacattc tggtggccac ccccggccgc ctgctggacc acgccagcca
     1381 gggcacgctg gatctgagcc aggtgcagat cctggtactc gacgaagccg atcgcatgc
//
LOCUS       DAIDFG010000438         1426 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_438_length_1426_cov_12.256747, whole genome shotgun sequence.
ACCESSION   DAIDFG010000438 DAIDFG010000000
VERSION     DAIDFG010000438.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_438_length_1426_cov_12.256747"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          214 a          475 c          488 g          249 t
ORIGIN      
        1 ccgagggggc gctgcgcctg cggcccggca aagccggttc cgcggcccct gctggtcaag
       61 agggagtcgc caatctcggg gctggtcttg cttggggcgg ccggcgcgtg gtgaaaatac
      121 ttcttgtcaa actctcgtcg ctcggcgacg tggtgcacac cttgccggtg gtgcaggaca
      181 tcctggcggc gcgccccggc gcgcagatcg actgggtggt ggaacaggcg tttgcgcctt
      241 tgctggcccc gctgctggtg agcggtcatg tgcagcacgt gatcccctgc gagttgcgcc
      301 gctggcgcaa cgcgccgctg gcggccgcca cgagggccga gtggcatatt ttcaaaaaac
      361 aactgcaggc ccagccgtac gacgcggtga tcgacctgca gggcctgacc aaatcggccg
      421 tggtggcgcg cctggcgcgg ctggcgccgg gcggcaagcg ttatgcgctg gccaaccaga
      481 ccgaaggctc ggcctacgag gcgccgacgc gctgggtcgc cgacgtggcg attgccatgg
      541 cgccgcacat tcacgccatg cagcgcggac gcgaactgtg cgcgcgcgcc ctgggctacg
      601 ccttgccgcc ggttccggat tttgggttaa aagtgccttc agcccttatg gaacgtgcgc
      661 aagatgctcc tgaaacagta gcatttgtac acggcacctc gcgcgccgac aagcagtggc
      721 cgctggaaaa ctggatcacg ctgggccgtc agttgatggc gcagggttac cgcattgcct
      781 tgccgcacgg cagcgccgac gaactggcga cgagccaggc cattgctgcg ggcttgaacg
      841 ccggaagcgc ggaagatcag gtcatcgtgt ggccccgcct tccgctcgat gcgctgacgc
      901 agcacctcgc gcagtgcgcc ggcgtcgtcg gcgtggacag cggcctgagc cacattgccg
      961 tcgcgctcga cctgccgcat gtgcagatct acaacttcga caccgcctgg cgtaccgggc
     1021 cactgcctga ttcgacaggc gcaggtgctc cgcgccagct cagcgttttt gcccagccca
     1081 caccgtcggt gcaggcggtc tgggacgcct ggctcagttg ccagccgctc ccggcgcagg
     1141 tgtccgaatg ggcgccgctt ccctgatgat ccgccagctt tattcactgt tgatgtgggc
     1201 ggcccagccc tttttgcggc gcaagctgcg caggcgcggc ctggccgagc cgggttacct
     1261 cgacgccatc gaggaacgct tcggccatta cacgcaagct gctgaaagca tctccgagct
     1321 ggtgtgggtg catgccgtgt cgctgggcga aacccgcacc gcgggcattt tgctggcggc
     1381 cctgcgcgcg cagtggcccg ggctgcgcat cctgctgacc cacggc
//
LOCUS       DAIDFG010000439         1369 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_439_length_1369_cov_16.356925, whole genome shotgun sequence.
ACCESSION   DAIDFG010000439 DAIDFG010000000
VERSION     DAIDFG010000439.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_439_length_1369_cov_16.356925"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          275 a          466 c          436 g          192 t
ORIGIN      
        1 ggcgcgctgg aaccatttca aattccgcct tggccgactg gagggtgtgg accgtgccgc
       61 caagaccgtg cagctggccg ccagccacga cgaggccggg cgcgagatca cgccggcgca
      121 gcagctcggc tacgacacgc tggtgattgc ggtgggcagc cagaccaacg acttcggtac
      181 cccgggcgcg gccgaacaca ccatcaaact ggacagcccg caggcggcca aacgtttcaa
      241 cgaccgcctg atcaatgcct gcatacgcgc acaggccgtg ccgcgcagcg ccggcagcgg
      301 ccgcctgacg gtggccattg tcggcggcgg tgccaccggg gtggaactgg ccgccgagct
      361 ccatgcctcg gccaaggtgc tggccaacta cggctttgac cacatccacc cggaaaagga
      421 cttgcagatc gtgctggtcg aagcggcgcc gcgcctgctt gcgcaactgc ccgagcggct
      481 gagcgacgcg gccctgcgcg aactgcgcaa gctggccatc gaggtgcaca ccaatgaaaa
      541 agtggtggaa gtcaccgcgg acagcctgaa gatggcgagc ggcaaggtga tttcctccag
      601 cctctcggtc tgggcggccg gcgtgaaggc agcggacttc ctcaaaaccc tgggcggcga
      661 tgcccccctg gagaccaacc ggctcaacca gctcgtcgtc agcggcaacc tgcagaccac
      721 ggatgccagc atctacgcct tcggcgactg cgccgcctgc caacagcccg acggcacctg
      781 ggtgccgccg cgcgcgcagg ccgcctacca gcaggccatg tacctggtca gggcgctgcc
      841 gcggctgcag cgcggcgagc cggtttcccc ctttgtgttc cgggaccagg gttcgctggt
      901 gtcgctgtcg gagtattcct cggtcggcag cctgatgggc agcctgacca aaggcagctt
      961 tttcatcgag gggcaactcg ccaagatcat gtactgggcg ctgcacaagc agcaccagct
     1021 ggcgctgggc ggctggaaaa aaaccgccct gatcacgctg tcggaaatga ttgaccgcac
     1081 ctatcgtccg cgcatcaagt tgcactgata atcagaccac atccccatca acccaccgga
     1141 gaacgccatg gcaaaaggac agcaaaacaa ggacaagatg gtcaagaagc ccaagaagga
     1201 cacggccccc ccgaaagcgg tgtcgcccga tgcggtgcgc ccgaccgtgg tcacgcaagt
     1261 ccccctgcgc ggcaagctga aaaacaaata aggcagtgcc ctctttacca gccagggccg
     1321 cggaaccggc tttgccgggc cgcaggcgca gcgccccctc ggggggcag
//
LOCUS       DAIDFG010000440         1351 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_440_length_1351_cov_17.359568, whole genome shotgun sequence.
ACCESSION   DAIDFG010000440 DAIDFG010000000
VERSION     DAIDFG010000440.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_440_length_1351_cov_17.359568"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          260 a          472 c          418 g          201 t
ORIGIN      
        1 gtgcccatgc agtgcgagta ctttgcgctc gaaggcctga cggatctggt caacaccatc
       61 aagcaggtgc acgccaacct caacaaggac ctggccatca tcggcctgct gcgtgtcatg
      121 ttcgacccgc gcatcaccct gcagcagcag gtcagcgacc agctgaaaga gcatttcggc
      181 gacaaggtgt tcaacaccgt gatcccgcgc aatgtgcgcc tggccgaagc gccgagttac
      241 ggcctccccg gcgtggtgtt tgacccgtcg tccaaaggcg cgcaggcttt cgtcaccttc
      301 gccgaagaga tggtgcagcg catcaaggcc atgtgatggc ccgggtttca agccaaatcg
      361 gcctccagcc caggtgcgac gtgcgctgac agctatcaaa accatagtaa acctgctttt
      421 cccggcatga agtcctcgaa cgtcctgatc ctccccggct ggcaaaacag cggccccgac
      481 cactggcaaa gccgctggga gcgggcgcac ggctaccagc gtgtggagca acacgactgg
      541 atgcacccgc tgcgcggcga ctggatcgca cggctcgaag aggtgctgct gtcctgcgac
      601 gagcctgccg tgctggtggc gcacagcctg ggctgcatac acacggcggc ctgggccacc
      661 cattcgaaaa acacgcagcg cgtcaagggc gcgctgctgg ttgcaccgcc cgacgtggag
      721 cgcgaggaca tccgccccat gctcagcagc tgggcgccgg tgccgctgca gccgctgcct
      781 tttccggccg tggtgtttgc cagcagtgac gacccgtttt gcgcgcccgc gcgggcggcg
      841 cagttcaccg ccgcctgggg cgccaggccc atcgacgccg gtgcacgcgg ccacctcaat
      901 gccgccagcg gcctggccga ctggcccgag gcccacgaac aactcatgcg gctgatacgt
      961 ctctgacgct tcgccaccct atctgaacca gagaaagaca agacaccatg gtcaccaaaa
     1021 aacccaaagg cctgggccgc ggactcgaag ccctgctggg ccccaaggtc agcgaagccg
     1081 gggacgccga caagggcaac agcagcgcca ccagccccgg ccagccggcc tcgctgctgc
     1141 tgaccgacct ggtcgccggc cagtaccagc cgcgcacccg catggacgag ggcgcgctct
     1201 acgaactggc tgagtccatc aaggtgcagg gcatcatgca gccgattctg gtgcggcgcc
     1261 tgaccagcgg cgccaacgac ggcaagtacg aaatcattgc cggcgagcgc cgtttccgtg
     1321 cggccaaaat cgccggcctg gacagcgtgc c
//
LOCUS       DAIDFG010000441         1344 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_441_length_1344_cov_16.442203, whole genome shotgun sequence.
ACCESSION   DAIDFG010000441 DAIDFG010000000
VERSION     DAIDFG010000441.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_441_length_1344_cov_16.442203"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          233 a          439 c          437 g          235 t
ORIGIN      
        1 acgcgcacat ccaccagtgc gagtgcctgc tttaatgcat ccgtcagaag acggtcggac
       61 tggctgggct cggctacccc cgagggatgg ttatgcgcga ggataaccgc cgctgcgttg
      121 tgaaacagcg cgcgtttgac cacttcgcgc gggtagacgc tggtttgtgt cagcgtgccg
      181 cgaaacagct cttccagcgc gatcagccgg tgctggctgt cgagaaacag caccgcaaaa
      241 atttcatgcg gccggctgcc cagctgcaac tgcaggtagt cgcgcaccgc ctgcggcgtc
      301 gtgaacagcg gtttgtcctg cagctcctgc gccagcgcgc ggcgtgccag ctccagcacg
      361 gccaccagct cggcgcgttt ggccggtccc aggcccttga tgcggttgag ggcctcatgc
      421 ccggtgtgca gcaggccggc caccccgccg aaagtgtcca ccaactcctg tcccatctgc
      481 agtgcgtttt tgccgggaat gccgctgcgc agcagcaggg ccagcagctc ggcgtcgctg
      541 agcgcgccgg ggccgcgggc cagcagtttt tcgcgggggc gggcgtcctc gggcaggtct
      601 ttcaggagca tgggcattcc ggcaggtggg caagcattga aaaaaacgcc ttcacagggc
      661 cagtatcagg gctaaacggg ctgttgtttc ccggacggtc ggcactttct acaatagggg
      721 cagtttacaa acagttggcg gcctcccggg ccggcacccg aaagtcctat gtccaccgtg
      781 gaaacactcg ctcccgccct tgtccagcct ggttccttcc tgaccctgca ctaccgcatg
      841 gccgggccgt ctgtggacgg aaaaccgggc gccgacatca tcaacacctt tgccggcaag
      901 cccgccacgc tgagcctggg caccggcgag ctgtcgcccg cgatcgagca gcgcctgctg
      961 ggcctgtgcg aaggcacgcg caccagcttc gagctggccg ccggcgaggc ttttggcgag
     1021 cgcaatcccg agctggtgca gtgggtggcg cgcaagctgc tcaatgaact gggcgacccc
     1081 cacgagcagt acaaggtcgg cgatgtcgtg cagttcccca cgcccgacgg catgggccag
     1141 tacgccggca gcgtgcagca ggtcaaggca gacggcgacg ccgtgcagtt tgacttcaac
     1201 cacccgctgg ccggccagcc ggtggtgttt gaagtccagg tcataggcgt tttatgaacg
     1261 acaaggcatt gccccaggaa atcatcctgg ccgaaccgcg cggtttctgc gccggtgtcg
     1321 accgcgcgat cgaaatcgtc gagc
//
LOCUS       DAIDFG010000442         1334 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_442_length_1334_cov_14.381548, whole genome shotgun sequence.
ACCESSION   DAIDFG010000442 DAIDFG010000000
VERSION     DAIDFG010000442.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_442_length_1334_cov_14.381548"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          252 a          447 c          413 g          222 t
ORIGIN      
        1 gataacgcgc gccgcccagg tgccggaact cgcggcatat gccaaggcgc tgggccaggc
       61 gctgcagcag gtcggcacga ccacccaggc cgcctgggcc accggcaacc cgcaggatgc
      121 gcaggccaac gccgtccctt atatgcaagg cttcggccac accgtgctgg cctggatctg
      181 gctcgatgtg gcgcttgccg cgctgcaggc cgatgctaca aaatccatag ctgttaccgc
      241 cggtaaattg ggcgcggtgc gctactttta tcactacgaa ttgcctaaaa tcgacgcttg
      301 gctccaggtg gttgcagacc gcgacctgac ctgtgccgac ttgccggagg aggcgttctg
      361 atgggatttt tcaaggaatc gggtagcacc acgtcgagca agtcgattgc ccggctgcag
      421 cagctgatct ggatcctgat ttacggcggg ctgctcacgc tggtgctggg cctgtcggtg
      481 cagcgcatgg atgccgccct cggctggtcc atggtggtcg ctggcggcat cgcggcggcc
      541 atcggttttg tgctgatcta cgtgcgttcc cgcctgacca gcagcgactg acccgtccgg
      601 cctgcgacag ccggcaggcg cgctttgcgc gacactcgtt cccatcttcc ccaacaacaa
      661 ggagcccctc atgacccgta ccatccagca gctgttcgac ctcaccggca agaccgccct
      721 cgtcaccggc ggttcacgcg gcctgggcct gcagctggcg caggcgctgg gcgaagccgg
      781 cgccaaaatc atgctgagtt cgcgcaaggc agaagacctc gaagagtcgg ccgccacgct
      841 gaaggcggcc ggcatagccg tggactggat cgctgccgat tgctcgaaag aagcggacat
      901 ccgcgcgctg gccgatgaaa ccatcaagcg cttcggccat gtagacatcc tcgtcaacaa
      961 tgcgggcgcc gcctggggcg cgccggccga agaccacccg gtcgaagcct gggacaaggt
     1021 catgaacctg aacatccgcg gctacttcat cctgagccag cacatcgcca aacacagcat
     1081 gatcgcgcgg cgctccggcc gcatcatcaa cgtggcctcc atcgccggcc tgggcggcaa
     1141 cccgcccgag atgaccaccg tggcctacaa cacgtccaaa ggggcggtga tcaacttcac
     1201 gcgcgcactg ggttgcgaat ggggcaagta caacatcacc gtcaacgcga tttgcccggg
     1261 ctttttcccg agcaaaatga cggtgggcac gctcaaggcc ctgggcgagg aaaaactggc
     1321 cgcccacgcg ccgc
//
LOCUS       DAIDFG010000443         1169 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_443_length_1169_cov_16.974865, whole genome shotgun sequence.
ACCESSION   DAIDFG010000443 DAIDFG010000000
VERSION     DAIDFG010000443.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_443_length_1169_cov_16.974865"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          245 a          350 c          318 g          256 t
ORIGIN      
        1 ccttcaccag taccttcccc accaagcggc cgtaacggtc gttcttctca ccctccacct
       61 ctacccacct tccgtaaacc aggtcggaca ggttttgctt ggagcgctgg ccgaaaggca
      121 tcctcttttc gggcgcatca ataccggaga ggcgtaccac atgctcgaac ttgttggcat
      181 ccagcacctt gacggtgtcc ccgtcggtca ctgctacgac cctgccttcc agaacggctg
      241 cagcggccac tatggaaagg cccagcgcca acatcgtgaa cagcaggcca cgccacgccc
      301 tgcggctaaa taaccgcgcg ggaaactgct gcatggctat tttcataccg agctatgaac
      361 caggaatgcg cgcgccgtgg tggcaatcac cttcctcgca ttggcggcga tatcgttggg
      421 aagaggcttg gcttgcgcgg cgggaaacgg ccacggcgga tgtcgccgga tgccgggcgg
      481 cagggacagc ggcacgaccg gggcacgggc cggctgggta tacaaagctt gcatggggtg
      541 gtttcggtcg gctctcatgg ggccaatcat cctactggac aactgtattt atgtacagta
      601 tcataaacac catggaagac atctctccca cccaatgggt tactcaatgc gctgagcgat
      661 tgcatcagcg ctggcacact gttgagtctg cacaattgga ggaggtagcg attgagcttt
      721 ggcaagactc gcatcttcgc gcattgccgc cagcagatgc agcagagctc tggctgagtc
      781 ctgtggccgg tcagcccgac tgacgcgtca accacttgac ctggatgtga ggtcagggtt
      841 tatgcgacaa aaaataattc atctgaacta tgcgctcaag cggccgccca gagttggcta
      901 tgcgcgttta gctctttgcc atgatcgcaa atagcagccc aagacctata aggacggctg
      961 ccttgttgtc ctcttttttg tccggtacgt ctacaccgct cttttcaagc cgaacagcca
     1021 gctcatctgc tcgctgcaga atttgatgag gcgcgcattt aagtttcgcg gcgatcaccc
     1081 gaagttgagc caacgtcatt gctgcttgtc ccgactccag tttggaatag cccgactgcc
     1141 ctagctctaa agccgcggcc atatctgac
//
LOCUS       DAIDFG010000444         1107 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_444_length_1107_cov_17.538973, whole genome shotgun sequence.
ACCESSION   DAIDFG010000444 DAIDFG010000000
VERSION     DAIDFG010000444.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_444_length_1107_cov_17.538973"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          214 a          336 c          355 g          202 t
ORIGIN      
        1 tgcgcaaggc gttcaagggg cacagcatga agcgggtcag catcagctac tcggtgggcg
       61 cttcagggcg cggccgggag cccaggggcg agctgatcat ctgcaacttc aagccaggtg
      121 cttgagaatg ccagaccatc gttgtctggc atgcaggaac cgcttgctgg ctgggccgaa
      181 cagagcgtta ctgggcgcat gaacaaaccg cccctcaccg ccctggccac cgcgcccgct
      241 gaccttcttg agcgcatcgc ccgcgaacaa ctcggcctcg aaaccctgat tgcccgcagg
      301 agcgacagcc tggattttca tgacctcgcg gtctggtcga ttgaaaaggc cctgcaggcg
      361 gcttacgctg ccggcaggca cgccgctgcg ggcgatgcgg tatgaagacg gcggaggcgg
      421 catacgctga ccaccaggcc gcagcaaagg cgcttttggc caggctggcc agagcggttg
      481 atgagcatgc cggcaaagcc aaggctcacc aaaccaactg gggctatgtc ggcgatctgg
      541 acggcctatg cgggcagctg atccaaggcc tcggcatgct ggacgcgctc accgaggccg
      601 agcggcagat ccaccgcttc tagccttaga attccccgct ggtcctacgg cgatctgccc
      661 gggctggctg aggccaaccc cttgccactc cgctgatcgc ggctcctagg ccgatctgag
      721 aggtcgtttt ccggcttggc ggggcgataa acacaggttt tagtgtgctt ttaaggcccc
      781 atagaggggc tttttatttc aagatttcgg acgcagtgtt acaaattgga cgcgaaagcc
      841 aaaattcagg atcatgcgtc cgcaagttca gcgccacgcg gccgcttaca ccttctggct
      901 gcgccgagga gcggagggcc agacggataa ggacgggcga ttgtctgagc gtagcgagtt
      961 cgagcccggc cccggctgga ccgagcaccg caggttgccc cggagcgtag cgcaggggtc
     1021 gcagacagca gggtcgcctt ttcttttgct tacttttctt ttggcgacgc aaaagaaaag
     1081 tgagttgccg ccgggcaacc cccggct
//
LOCUS       DAIDFG010000445          962 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_445_length_962_cov_14.855568, whole genome shotgun sequence.
ACCESSION   DAIDFG010000445 DAIDFG010000000
VERSION     DAIDFG010000445.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_445_length_962_cov_14.855568"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          222 a          286 c          305 g          149 t
ORIGIN      
        1 ccctggagga aaagcaccag catgcgcgcc aggcgctgga gcatttccga caatcgagca
       61 aggaacagcg cgatcaggac cagcgcaagc acgagcagca ggtgcagtac ctgcaggccg
      121 agttacgcac cgtgaacgaa accttggcca ccaagcagca ggaagccgtc cacaccctgc
      181 aggaaaacgc gcgcctgctg ggcgatttgt cgcgcgccca aggcgatctg caccaggccc
      241 aggaagaggt gcggggcctg cggccgctca aggatgaact gggatttgcg caaaggcgcg
      301 ccgaggaatt gggccggcgc ctggtcgagc aggacgcggc cgtccagcgg ttcagcgcct
      361 cgaacgagca gttgcacgct aaggtcgatg aactgctcac tgcgaagcag cagctggaac
      421 tggcgctggc gactgccagg tcatccgtca cggcgcaaga gcaggtcgtg gccagcatgc
      481 tcgagcgctt cagtgcgccg gcgcctggtc ctaatgtggt tacgaacctg gctgatgggt
      541 ccgttcgttc agcgaaggga gagtcagaaa aatcaacggg gacagggact taagcttatt
      601 ttaccttttc gagggaatgg gctcatttag ccacattgca ccgaatcacc tgtagcggga
      661 cttccaggct gacggcccga accagaagtg ggtggccgac tacacctgca tctggacggc
      721 cgagggctgg ctgtatgcgg cagccgtgca aagtagcgaa gatttcaatg accgcaccac
      781 ccgcacccga ccaagaagag atgatcggca atggctcctc cgctcagcca gaaaaagagc
      841 acgcaaccgg cgaggtcggc gtccgagcca aagagggatg gtcggctcgc atgcaacatg
      901 ggcgctattc acgccgctga ccacttaagt tcacgccgct gaccacttaa gttcacgccg
      961 ct
//
LOCUS       DAIDFG010000446          706 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_446_length_706_cov_21.529954, whole genome shotgun sequence.
ACCESSION   DAIDFG010000446 DAIDFG010000000
VERSION     DAIDFG010000446.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_446_length_706_cov_21.529954"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          124 a          197 c          253 g          132 t
ORIGIN      
        1 agttccggcg ggatggtgag cttgcgcttg ccgccgatct gcatgccggc cacgccctgg
       61 tcccagcccg aaatgacctg gcctgcgccg aggcggaatt cgaacgggtc gttgcggtcg
      121 acgctggagt cgaatttctt gccgtcggtg agccagccgg tgtaatgcac ggtcacggtt
      181 tgccctgcgg tggcggtggc gccgctgccg gcgacgaggt cttcaatgat gagttcgctc
      241 atggattcgg tccctggaga gtaaggtttg caattgacgt gcatctgaca ggcgatgcgc
      301 gaagtcgttc attttctcac gtttttcacc tggggcgaac cggggttcag cgccactcgg
      361 ccggcaggta gtaggcgtcg ggatcgacgt cgatggggat ttcggtgacg ctgctggcgt
      421 ccggcggcgg atcgcgcagc accacctgcg ggaaggccag caccaccgcg accatgaaca
      481 gctggatcgc caggaaggga atcgcgcccc agtagatgtc ggcggtgcgg atggctttcg
      541 gcgcgacgct cttcaggtag aacagggcga agccgaaggg cgggtgcatg aaggcggtct
      601 gcatgttgac cgcgagcatg acgccgaacc agatcgggtc gatgccgagc ttgtaggcga
      661 tcggcgccag cagcggcacc acgatgaagg cgatctcgaa gaagtc
//
LOCUS       DAIDFG010000447          570 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_447_length_570_cov_31.153398, whole genome shotgun sequence.
ACCESSION   DAIDFG010000447 DAIDFG010000000
VERSION     DAIDFG010000447.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_447_length_570_cov_31.153398"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          119 a          199 c          154 g           98 t
ORIGIN      
        1 gaaatacgac gagattgaca gttcgcctga agagaaggcg cgcggcatca ccatcaacac
       61 cgcgcacgtc gagtacgaga ccgccaagcg tcactacgct cacgtcgact gcccggggca
      121 cgccgactac atcaagaaca tgattaccgg tgctgcccag atggacggcg ccatcctggt
      181 cgtgtcctct gctgacggcc ccatgccgca gacccgcgag cacatcctgc ttgcgcgtca
      241 ggttggcgtg ccgtacatca tcgtctatat gaacaaggcc gacatggtcg acgacgccga
      301 actgctcgag ctcgtcgaaa tggaaattcg cgaactgctc agcaagtacg actttcccgg
      361 cgacgacacc cccatcatca tcggttccgc cctcaaggcc ctcgaaggtg accagagcga
      421 catcggcgag cccagcatcc tcaagctggc ggaagccctc gactcctaca tccccgagcc
      481 tgagcgcgcg gttgaccgtc ccttcctgat gccggtcgaa gacgtcttct ccatctccgg
      541 ccgcggcacc gtcgtcaccg gtcgtgtcga
//
LOCUS       DAIDFG010000448          540 bp    DNA     linear   ENV 27-DEC-2022
DEFINITION  MAG TPA_asm: Polaromonas sp. isolate clean6305
            NODE_448_length_540_cov_20.115464, whole genome shotgun sequence.
ACCESSION   DAIDFG010000448 DAIDFG010000000
VERSION     DAIDFG010000448.1
DBLINK      BioProject: PRJNA834572
            BioSample: SAMN28081315
            Sequence Read Archive: SRR5352529
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polaromonas sp. (mine drainage metagenome)
  ORGANISM  Polaromonas sp.
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Polaromonas.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Wang,L., Liu,W., Liang,J., Zhao,L., Li,Q., Zhou,C., Cen,H., Weng,Q.
            and Zhang,G.
  TITLE     Mining of novel secondary metabolite biosynthetic gene clusters
            from acid mine drainage
  JOURNAL   Sci Data 9 (1), 760 (2022)
   PUBMED   36494363
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  AUTHORS   Zhang,G., Liu,W. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2022) an eLibrary of Microbial Systematics and
            Genomics, 320 Yueyang Road, Shanghai 201203, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: MEGAHIT v. V1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 12x
            Sequencing Technology  :: Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Polaromonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_448_length_540_cov_20.115464"
                     /isolate="clean6305"
                     /isolation_source="water"
                     /db_xref="taxon:1869339"
                     /environmental_sample
                     /country="Canada: Ontario"
                     /lat_lon="46.65 N 81.32 W"
                     /collection_date="2014-09"
                     /metagenome_source="mine drainage metagenome"
                     /note="metagenomic"
BASE COUNT          103 a          167 c          171 g           99 t
ORIGIN      
        1 gaaccacgga actcgccttc gagctcctgg atgatcttgc cgttgcctcg caccgggccg
       61 tccaggcgct gcaggttgca gttgatgacg aagatgaggt tgtcgaggtt ctcgcgcgcc
      121 gccagactga tcgcacccag cgattccacc tcgtccatct cgccgtcgcc gcagaacacc
      181 cagaccttgc ggttctcggt gttggcaatg ccgcgcgcat gcaggtactt gaggaaacgc
      241 gcctggtaaa tcgccatcag cgggcccagg cccatggaga cggtggggaa ctgccagaac
      301 tcgggcatca gcttgggatg cgggtaactc gacaggccct tgccgtcgac ttcctggcgg
      361 aagttcagca gttgctcttc gctgagccgc ccttcgaggt aggcgcgggc atacacaccg
      421 ggcgatacat gaccctggat atagaggcag tcgccgccat gatcgtcgct ttccgcgtgc
      481 cagaaatggt tgaagccggc gccgaacagg ctggccagcg aagcgaacga gccaatgtgg
//
